Happy New Year to all and especially all who responded to my call for help. Thanks again. Best wishes for a great year. Sincerely, Peter A. Stolzenberg,Pesto Inc.
sounds like a research grant to me. I don't believe the salary of faculty in Chemistry departments of most major colleges would be "soft money" requiring grant support. I commend the Society of Analytical Chemists for trying to encourage this bridge. 20K of seed money for a new line of research can make an important difference to a junior faculty member.
} ---------------------------------------------------------------. } } } Hum....so for a 2000 hour year, this works out to be $10 per hour. } What a gift. And they probably want $30/hour of work in return. } } No matter how you package it, all of this babble does not measure } up to today's standards. Unless SEM, etc. is a obscure and } diminutive endeavor, I simply do not understand the cost-benefit } ratio. Maybe this is not an annual salary. OK. Is this in addition } to an existing income stream? Geeze, I hope it is the latter. But it } sounds like the position is on-site. So, the candidate gets a full time } job at McDonald's as well? } } All I can say is that I am glad and relieved that I do not have to } work and try to survive in this type of environment. Welcome to H-2 visas. } } gary g. } } } At 08:14 AM 12/31/99 , you wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I just received this notification from the Society for Analytical Chemists } } of Pittsburgh. Perhaps it will provide an important start for a new chem } } prof who has an interest in expanding the use of microscopy and/or in } } walking across the new bridge between microscopy and spectroscopy: } } } } "Twenty-first annual Analytical Chemistry Starter Grant Award" } } The society for Analytical Chemists of Pittsburgh will award one grant of } } $20,000 to an assistant professor in the field of analytical chemistry. } } The purpose of this grant is to encourage high-quality, innovative research } } by a new analytical chemistry professor and to promote the training and } } development of graduate students in this field. Assistant professors who } } have accepted a US college or university appoint since December 31, 1996 } } are eligible. Application forms available from: } } James Chadwick, Chairman } } Starter Grant Committee } } Society for Analytical Chemists of Pittsburgh } } 200 Penn Center Blvd., Suite 332 } } Pittsburgh, PA 15235 } } Ph: 1-800-825-3221, Xt. 208 } } Fx: 412-825-3224 } } } } Deadline for application receipt: February 29, 2000 } } Award winner announced: May 1, 2000 } } } } } } Best regards and welcome to the new millennium! } } Barbara Foster } } Consortium President } } Microscopy/Microscopy Education ...Educating microscopists for greater } } productivity. } } } } 125 Paridon Street Suite 102 Springfield, MA 01118 } } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } } Visit our web site {http://www.MME-Microscopy.com/education} } } ****************************************************** } } MME is America's first national consortium providing } } customized on-site workshops in all areas of } } microscopy, sample preparation, and image analysis.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
Thanks for the info. I understand what is going on now. sounds like a great opportunity.
I discussed compensation before and don't want to clutter up the list with a repeat. But I would appreciate an off-list communication which helps me better understand how the mechanics of SEM and its personnel work in the big picture. I get asked often how to get into the field. Since I use my SEM for personal research and photography only, I am not in academia or companys' formal research departments. If I sound ignorant about this, it is because I am. I work with some high school and upper level pre-high school students at times using my SEM and LMs. What can be done with the SEM and LMs turns them on as much as it still does me today. I'm hopelessly hooked.
But I lack facts and figures about this type of career. Rather than speculating about it, what do you careerists think? Is this a good career? What is involved in getting into the career at various levels? And what compensation is commensurate at these levels?
All responses will be kept confidential. Use PGP if you need to.
gary g.
At 12:39 PM 1/1/00 , you wrote: } sounds like a research grant to me. I don't believe the salary of faculty in Chemistry departments of most major colleges would be "soft money" requiring grant support. I commend the Society of Analytical Chemists for trying to encourage this bridge. 20K of seed money for a new line of research can make an important difference to a junior faculty member. } } } } ---------------------------------------------------------------. } } } } } } Hum....so for a 2000 hour year, this works out to be $10 per hour. } } What a gift. And they probably want $30/hour of work in return. } } } } No matter how you package it, all of this babble does not measure } } up to today's standards. Unless SEM, etc. is a obscure and } } diminutive endeavor, I simply do not understand the cost-benefit } } ratio. Maybe this is not an annual salary. OK. Is this in addition } } to an existing income stream? Geeze, I hope it is the latter. But it } } sounds like the position is on-site. So, the candidate gets a full time } } job at McDonald's as well? } } } } All I can say is that I am glad and relieved that I do not have to } } work and try to survive in this type of environment. Welcome to H-2 visas. } } } } gary g. } } } } } } At 08:14 AM 12/31/99 , you wrote: } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } I just received this notification from the Society for Analytical Chemists } } } of Pittsburgh. Perhaps it will provide an important start for a new chem } } } prof who has an interest in exd, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
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Thanks for the info. I understand what is going on now. sounds like a great opportunity.
I discussed compensation before and don't want to clutter up the list with a repeat. But I would appreciate an off-list communication which helps me better understand how the mechanics of SEM and its personnel work in the big picture. I get asked often how to get into the field. Since I use my SEM for personal research and photography only, I am not in academia or companys' formal research departments. If I sound ignorant about this, it is because I am. I work with some high school and upper level pre-high school students at times using my SEM and LMs. What can be done with the SEM and LMs turns them on as much as it still does me today. I'm hopelessly hooked.
But I lack facts and figures about this type of career. Rather than speculating about it, what do you careerists think? Is this a good career? What is involved in getting into the career at various levels? And what compensation is commensurate at these levels?
All responses will be kept confidential. Use PGP if you need to.
gary g.
At 12:39 PM 1/1/00 , you wrote: } sounds like a research grant to me. I don't believe the salary of faculty in Chemistry departments of most major colleges would be "soft money" requiring grant support. I commend the Society of Analytical Chemists for trying to encourage this bridge. 20K of seed money for a new line of research can make an important difference to a junior faculty member. } } } } ---------------------------------------------------------------. } } } } } } Hum....so for a 2000 hour year, this works out to be $10 per hour. } } What a gift. And they probably want $30/hour of work in return. } } } } No matter how you package it, all of this babble does not measure } } up to today's standards. Unless SEM, etc. is a obscure and } } diminutive endeavor, I simply do not understand the cost-benefit } } ratio. Maybe this is not an annual salary. OK. Is this in addition } } to an existing income stream? Geeze, I hope it is the latter. But it } } sounds like the position is on-site. So, the candidate gets a full time } } job at McDonald's as well? } } } } All I can say is that I am glad and relieved that I do not have to } } work and try to survive in this type of environment. Welcome to H-2 visas. } } } } gary g. } } } } } } At 08:14 AM 12/31/99 , you wrote: } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } I just received this notification from the Society for Analytical Chemists } } } of Pittsburgh. Perhaps it will provide an important start for a new chem } } } prof who has an interest in expanding the use of microscopy and/or in } } } walking across the new bridge between microscopy and spectroscopy: } } } } } } "Twenty-first annual Analytical Chemistry Starter Grant Award" } } } The society for Analytical Chemists of Pittsburgh will award one grant of } } } $20,000 to an assistant professor in the field of analytical chemistry. } } } The purpose of this grant is to encourage high-quality, innovative research } } } by a new analytical chemistry professor and to promote the training and } } } development of graduate students in this field. Assistant professors who } } } have accepted a US college or university appoint since December 31, 1996 } } } are eligible. Application forms available from: } } } James Chadwick, Chairman } } } Starter Grant Committee } } } Society for Analytical Chemists of Pittsburgh } } } 200 Penn Center Blvd., Suite 332 } } } Pittsburgh, PA 15235 } } } Ph: 1-800-825-3221, Xt. 208 } } } Fx: 412-825-3224 } } } } } } Deadline for application receipt: February 29, 2000 } } } Award winner announced: May 1, 2000 } } } } } } } } } Best regards and welcome to the new millennium! } } } Barbara Foster } } } Consortium President } } } Microscopy/Microscopy Education ...Educating microscopists for greater } } } productivity. } } } } } } 125 Paridon Street Suite 102 Springfield, MA 01118 } } } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } } } Visit our web site {http://www.MME-Microscopy.com/education} } } } ****************************************************** } } } MME is America's first national consortium providing } } } customized on-site workshops in all areas of } } } microscopy, sample preparation, and image analysis. } } Thomas E. Phillips, Ph.D. } Associate Professor of Biological Sciences } Director, Molecular Cytology Core Facility } } 3 Tucker Hall } Division of Biological Sciences } University of Missouri } Columbia, MO 65211-7400 } (573)-882-4712 (voice) } (573)-882-0123 (fax)
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I am posting this for the NY Microscopical Society.
This is a Very Good Course, at a Great Price.
Best Regards
Joseph Passero mailto:jp-at-spacelab.net
New York Microscopical Society -- Course Announcement ====================================================
Bernard Friedman Memorial Workshop
Polarized Light Microscopy
April 8, 9, 15 & 16, 2000
An advanced course on polarized light microscopy which will cover the following topics:
The nature of polarized light
The origin and interpretation of interference colors
Birefringence and crystal orientation
The Indicatrix
Compensation and variable compensators
Interference figures and their interpretation
The workshop will consist of two consecutive weekends of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy.
The course instructors include;
Jan Hinsch of Leica, Inc.
John Reffner of Trace Consulting
N.Y.M.S. Instructor Donald O'Leary.
WHEN: April 8, 9, 15 & 16, 2000 from 10 A.M. to 4 P.M.
WHERE:
New York Microscopical Society Facility 1244 McBride Avenue West Paterson, NJ.
Phone (973) 812-8377
Web Site URL: http://www.nyms.org
(The facility has free parking and is accessible by public transportation, Information on car pools and transportation will be provided.)
COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership) Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use.
HOW: Register using the form below. Limited to the first 12 registrants.
Thanks for your on-target observations ...and especially for getting Gary into a more positive perspective!
Best regards,
Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} At 02:39 PM 1/1/00 -0600, Tom Phillips wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I am looking for any information (year of manufacture, company history, etc.) about a Vickers Instruments, M1500974C microscope. I would especially like to acquire a copy of the owners manual if possible. Please look at the following pictures for further identification.
American Chemical Society, "Applied Optical Microscopy"
3 days of exciting interactive lectures, lab, and demos on all aspects of Light Microscopy, with a touch of video imaging Learn how to -match optics to your application -interpret images from a variety of contrast techniques -troubleshoot for artifacts and misinformation -do simple measurement -put a camera system on your microscope
New Orleans Hyatt Regency, Mar 10-12,2000 Tuition, books, coffee breaks: $895 for ACS members, $995 for non-members
While many of the examples used will be from materials science, this course is NOT LIMITED TO CHEMISTS! Biologists are also welcome.
For a syllabus and enrollment information, visit MME's website: www.MME-Microscopy.com/education (B. Foster is course coordinator)
Please reserve early. This course is given in conjunction with Pittcon, the biggest analytical meeting in the country. Rooms fill up early.
Best regards .... and welcome to the positive side of Y2K!
Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education}
} I am forwarding this from someone who sent this to me. If anyone has any } ideas for this person, please contact him through his email address which } is listed. } } ML } } } From: "rwinn" {rwinn-at-mweb.co.za} } } To: {wong-at-msg.ucsf.edu} } } Subject: CHROMOSOME 5 (5P-) } } Date: Fri, 31 Dec 1999 07:21:48 +0200 } } MIME-Version: 1.0 } } X-Priority: 3 } } } } DEAR, MEI LEI WONG } } } } MY NAME IS RENEY WINN AND I AM MAILING YOU FROM SOUTH AFRICA. } } I AM 25 YRS OLD AND HAS A BABY BOY OF 14 MTHS. } } } } I FOUND OUT THAT HE HAS CRI-DU-CHAT SYNDROME WHICH IS ALSO CALLED CAT-CRY } } SYNDROME. HE HAS A DELETION ON THE SHORT ARM OF CHROMOSOME 5. HIS } } DELETION ON THE SHORT ARM IS FROM THE 14.2 MARK WHICH EXTENDS TO THE 15. } } MARK. } } } } I KNOW THAT THIS IS A VERY RARE SYNDROME AND HAVE BEEN IN TOUCH WITH THE } } SUPPORT GROUPS IN AUSTRALIA AND U.K. HOWEVER.. } } I STILL FEEL THAT I NEED MORE HELP FROM LABRORATRIES OR SCIENTISTS. I AM } } TRYING TO FIND OUT MORE ABOUT THE SEVERITY OF MY SON'S CONDITION. I AM } } TRYING TO FIND OUT HOW BADLY HE WILL BE AFFECTED BY HIS DELETION. I WOULD } } JUST LIKE TO KNOW HOW HIS DELETION SIVERITY WOULD BE CLASIFIED AS. MAYBE } } ON A SCALE OF 1-10 ONE AS BEING NONE SIVERE AND 10 AS MOST SEVERE. THIS } } WAY I WOULD BE ABLE TO UNDERSTAND +- HOW SEVERE MY CHILD'S CONDITION IS. I } } WOULD ALSO APPRECIATE IT IF YOU CAN TELL ME MORE OR LESS WHAT TO EXPECT } } WITH HIS DELETION. } } } } MEI, IF THIS IS NOT SOMETHING YOU CAN HELP ME WITH, COULD YOU PLSE } } FORWARD THIS MAIL TO SOMEONE WHO COULD PLSE. I AM VERY DESPERATE TO FIND } } OUT MORE ABOUT THE SEVERITY OF MY CHILDS DELETION. THIS WOULD BE MUCH } } APPRECIATED. } } } } BRGDS } } RENEY WINN. } } rwinn-at-mweb.co.z Mei Lie Wong Electron Microscope Laboratory Department of Biochemistry HHMI-UCSF Ph. 415-476-4441 Fax 415-476-1902 http://util.ucsf.edu/agard/wong/index.html email wong-at-msg.ucsf.edu
I know this is a little off the microscopy subject, but in the interest of encouraging scientific exploration in a young person. I hope you will indulge me.
I'm looking for materials for my daughter's science project. She is testing the effectiveness of different mouthwashes in killing bacteria found in the mouth. Her experiment matrix requires 12 petri dishes with a bacterial growth medium. I can probably scrounge something to use as petri dishes, but the growth medium is a problem. I'm not even sure what it is properly called or what its makeup is. Is it something we can easily purchase locally, or even better is there a recipe for making a suitable substitute from ingredients found in the home?
Any and all suggestions will be greatly appreciated.
The New York State Center for Advanced Thin Film Technology The University at Albany - State University at New York
The Center for Advanced Thin Film Technology at the University at Albany - SUNY seeks applicants for a postdoctoral position with established expertise in the area of analytical electron microscopy. Demonstrated experience in the development and application of SEM and/or TEM based analyses, including chemical analysis, is required to achieve targeted technique development goals and to support an expanding microscopy base at the Center. Experience with, or interest in, scanning probe microscopy techniques is also desirable. Candidates with a Ph.D. in applied physics, materials science and engineering, chemistry or related fields are encouraged to apply. This appointment is part of an ongoing multi-year investment in personnel and new laboratory facilities for thin film technology research areas at the Center.
The Center is a New York State sponsored high technology research and development center with a $60M state-of-the-art infrastructure, including class 1 clean rooms for back end IC manufacturing on 200 mm wafers, and advanced processing, patterning and characterization capabilities for single and multi-layered thin films. The Center is currently assembling a 200 mm wafer facility for MEMS integration. In addition, a new facility that will house a 25,000 ft2 class 1cleanroom for 300 mm wafer pilot manufacturing and workforce training is presently under construction.
Please send a letter of application including a summary of research interests, curriculum vitae, and three letters of recommendation to:
William Harris, Director of Analytical Sciences NYS Center for Advanced Thin Film Technology CESTM B110 251 Fuller Road Albany, NY 12203
The SUNY/Research Foundation is an Equal Opportunity/Affirmative Action Employer. All positions contingent upon availability of funding.
The New York State Center for Advanced Thin Film Technology The University at Albany - State University at New York
The New York State Center for Advanced Thin Film Technology at the University at Albany - SUNY is a fast growing, high technology research and development program with a mission of supporting industry and creating new jobs. This position will serve as the primary infrastructure support person for the Center's scientific and technical staff.
Job responsibilities include: performing routine maintenance and calibration of a variety of surface spectroscopic and electron microscopy instruments; performing analytical work with these instruments; assisting students with the operation of this equipment; diagnosing and repairing non-routine problems with the instrumentation; and maintaining an adequate store of spare parts and supplies.
The position requires: an associates degree in electronics, computer science, or related technology and two to four years of directly relevant experience; a working knowledge of high vacuum systems; proficiency with related computer hardware/software; demonstrated ability to work in a high energy, team oriented environment; and excellent communication and analytical skills. Experience with surface analysis and/or electron microscopy instrumentation is highly desirable.
Please send a letter of application including a summary of research interests, curriculum vitae, and three letters of recommendation to:
William Harris, Director of Analytical Sciences NYS Center for Advanced Thin Film Technology CESTM B110 251 Fuller Road Albany, NY 12203
The SUNY/Research Foundation is an Equal Opportunity/Affirmative Action Employer. All positions contingent upon availability of funding.
I am working on a spectra plotting program in Visual Basic 6 that is just about ready for general release. I was hoping to put it out there in time for the M&M MM meeting.
It started out just as a program that would take EMSA formatted EELS data from Gatan's ELP and convert them into an Excel compatible file so that I bring the file to a PC. Then I got carried away and used it to try to learn Visual Basic. Currently, the program will open and overlay up to five spectra, plot them in linear, log or rescale them and print them. They can also be copied to the clipboard and pasted in other applications. They can be re-colored and a couple other things as well. There is even a help file!
I am trying to make the program more general and make it completely compatible with the EMMFF standard for all spectra, EDS and EELS alike.I have a couple of requests for information.
1) I would like to have some EELS spectra of different elements in EMMFF format to test it out and include in a distribution packet that I can put on the MAS-LIB. I am particularly interested in transition metal oxides. Please send them to me if you have them and I could put them in the deployment file. Who knows, this could be a poor man's EELS atlas.
2) I would like to know how much interest there is in this program.
3) What features would you like in such a program. It doesn't do any analysis yet, but it may in the future.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
I have a multi-element standards mount which over time has deteriorated and become contaminated with salts. Three of the standards have fallen out of the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in 1982, and it appears that this company no longer exists. I am interested in finding someone to re-polish the mount and replace the lost standards. Does anyone know of such a company/person?
Thanks in advance,
Jean M. Howard Reynolds Metals Company Materials Characterization-Electron Microscopy E-mail: jmhoward-at-rmc.com Office: 804.751.2554
Tina, I have not been reading all posts so you may have been provided with all the help you needed, but I thought I would respond just in case... We do a lot of work with similarly embedded specimens. The problem you have is very familiar to me, and I believe you are right in your approach to "Hold the samples in a vacuum for some period of time before putting them into the scope" This is the best method that I have been able to come up with. Assuming: 1) the resin is fully cured, 2) you have vacuum infiltrated the resin into the specimen as thoroughly as practical, and 3) you have minimized the size of high surface area specimens prior to embedding, then there is not much more to try. In my experience the outgassing is most often the result of contaminants within the porosity of the specimen, and is not the result of the resin. If you still feel the need to check this out further, you might try an embedding procedure using a thermosetting resin (such as Bakelite) rather than a thermoplastic (epoxy).
If the sample prep involves polishing, then the source of the contamination is probably the water or oil lubricant in that procedure. Minimize these contaminants by preparing the smallest, and best vacuum infiltrated specimen with as little porosity as possible. I do this by repetitive evacuation/N2 back filling (with heat if possible) prior to embedding. This "clears the way" for better vacuum infiltration.
I have had polymerization problems with some resin/material combinations, and I have usually overcome these by choosing a different resin polymer. Epoxies and acrylics, for example, behave quite differently on various substrates, so I will try LR White if I am having trouble with epoxy, and visa versa. If you use LR White for the initial embedding, keep the reaction volume small, and re-embed in a larger mount if necessary. I do not know if there are any good references on the subject of vacuum infiltration for porous materials, but these are the approaches I have learned to take. Good Luck! Brad Huggins
} ---------- } From: Tina Carvalho[SMTP:tina-at-pbrc.hawaii.edu] } Sent: Tuesday, December 28, 1999 2:23 PM } To: Microscopy Listserver } Subject: SEM - epoxy mountants } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, all- } } We have a Hitachi S-800 FESEM that is mostly used for biological } specimens. However, recently we have had a number of planetary geologists } looking at sections of meteorites mounted on epoxy resin. About the time } they started using the scope, we started having a number of problems that } suggest that we are getting some outgassing and contamination. I'm not } surprised; this happened before when looking at fish ear bones in similar } resin, despite the resin manufacturer's clain their product was completely } stable in the high vacuum and under the beam. } } My question is for those of you who routinely look at such samples: What } do you do to minimize the potential problems? Hold the samples in a } vacuum for some period of time before putting them into the scope? Paint } the exposed epoxy areas with carbon paint or similar? For the biological } samples we only require that the specimens be held over dessicant } overnight, but I suspect that more heroic measures must be taken for these } samples. } } Happy New Year to all! } } Tina } } ************************************************************************** } ** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } ************************************************************************** } ** } }
Can someone suggest a reliable manufacturer for LaB6 filaments? I need the sturdiest, most poor-vacuum-tolerant variety for a Philips EM400T in a student lab! and I figure that users know best which filaments perform well in the real world.
Also, I am looking for a source for Philips bulk-sample mounts, the type used to carry SEM samples for the 6485/STEM system. (I need the mounts, not the specimen rod.) I need both low-background carbon (preferred) or beryllium for EDS, as well as standard copper carriers. My usual sources tell me they have not stocked these items for years. Maybe someone has some sitting unused in a cabinet somewhere?? or knows of a current supplier.
Offlist replies preferred, so as not to clog up the works... will summarize and send info to others who are interested.
Thanks for your help.
Ann Hein-Lehman Trinity College Hartford, CT 860-297-4289 ann.lehman-at-trincoll.edu
I have a side question spurred by Scott Walck's post. Is there a simple low cost program already available that would just allow me to view and print EMSA format spectra under a Windows PC environment? I found some info. in the archives regarding the EMMPDL site and the EMMFF software. I even got so far as to download the sourcecode. But, I don't have a compiler to turn it into an executable. Even so, I'm not sure from the documentation whether it will do what I want. Also I came across NIST's DTSA but that's only for Mac.
Any suggestions out there? And if not, then to Scott yes there is some interest from myself! Thanks, Karen Zaruba
"Walck. Scott D." wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear all, } } I am working on a spectra plotting program in Visual Basic 6 that is just } about ready for general release. I was hoping to put it out there in time } for the M&M MM meeting. } } It started out just as a program that would take EMSA formatted EELS data } from Gatan's ELP and convert them into an Excel compatible file so that I } bring the file to a PC. Then I got carried away and used it to try to learn } Visual Basic. Currently, the program will open and overlay up to five } spectra, plot them in linear, log or rescale them and print them. They can } also be copied to the clipboard and pasted in other applications. They can } be re-colored and a couple other things as well. There is even a help } file! } } I am trying to make the program more general and make it completely } compatible with the EMMFF standard for all spectra, EDS and EELS alike.I } have a couple of requests for information. } } 1) I would like to have some EELS spectra of different elements in EMMFF } format to test it out and include in a distribution packet that I can put on } the MAS-LIB. I am particularly interested in transition metal oxides. } Please send them to me if you have them and I could put them in the } deployment file. Who knows, this could be a poor man's EELS atlas. } } 2) I would like to know how much interest there is in this program. } } 3) What features would you like in such a program. It doesn't do any } analysis yet, but it may in the future. } } -Scott } } Scott D. Walck, Ph.D. } PPG Industries, Inc. } Glass Technology Center } Guys Run Rd. (packages) } P. O. Box 11472 (letters) } Pittsburgh, PA 15238-0472 } } Walck-at-PPG.com } } (412) 820-8651 (office) } (412) 820-8161 (fax)
-- Karen S. Zaruba kszaruba-at-mmm.com 3M Company, St. Paul, MN
As one of the co-authors of the MSA/MAS File format we had a goal to make the format simple enough to be useable in even a simple spreadsheet program.
A number of the major manufacturer's allow you to translate their data files into this format directly from their application programs. To use the data in a spreadsheet, simply specify dual column (x,y) format for the output file and then you can import the data files directly into a MS Excel spreadsheet, or even better a data graphing program like KaleidaGraph (which runs on both Mac's & PC's). Then plot to your hearts content.
Hi, I've been working on thin foils of CZT and I'm looking for some suggestions to help eliminate artifacts created by ion milling. So far I have polished side one and etched (to remove polishing damage) with bromine-methanol and then dimpled side two, ending with 0.1um alumina. I have been successful obtaining a final sample thickness of about 8 to 10 microns. However, when I put the samples into the PIPs (Gatan) to complete the thinning process I'm seeing beam damage. I was hoping that someone might have a different approach or suggestion that would eliminate the beam damage. Thanks for your help. Dorrance
I have an investigator who would like to stain just the cell wall of the yeast S.cerevisae to distinguish it from the cell membrane at the EM level. Any tips or references willbe appreciated. Many thanks
Manuela Palatsides Electron Microscopy Peter MacCallum Cancer Institute Locked Bag#1 A'Beckett Street Melbourne 3000
On Mon, 03 Jan 2000 18:44:05 -0800, Mike Southwell wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello all, } } I know this is a little off the microscopy subject, but in the interest } of encouraging scientific exploration in a young person. I hope you will } indulge me. } } I'm looking for materials for my daughter's science project. She is } testing the effectiveness of different mouthwashes in killing bacteria } found in the mouth. Her experiment matrix requires 12 petri dishes with } a bacterial growth medium. I can probably scrounge something to use as } petri dishes, but the growth medium is a problem. I'm not even sure } what it is properly called or what its makeup is. Is it something we } can easily purchase locally, or even better is there a recipe for making } a suitable substitute from ingredients found in the home? } } Any and all suggestions will be greatly appreciated. } } Michael Southwell } JEOL USA INC. } Austin, TX } } Michael - I'm sure that any bacterial growth medium requries agar or, at home, gelatin. If you were considering making it at home, then buy some clear gelatin. You'll need to boil water first, though, and then add the gelatin to make it liquid. Before it cools, pour the gelatin along with the apprpriate growth medium into the petri plates (typically, a plate holds about 10 ml of medium). This substance (err, the gelatin) acts primarily as a hardener for the actual medium. It is generally considered to be non-nutritive for most bacteria, although I believe that some may consider it to be nutritious (spelling ?). As for the main nutrients ... I've been dealing primarily with protozoa, but I'm pretty sure that bacteria can grow on an extract of boiled lettuce or even wheat grains, etc. I cannot tell you from my own experience, however, whether a boiled extract in combination with gelatin does indeed work as a suitable growth medium, only that I've heard that gelatin works and that, in my experience, a boiled extract works well for supporting bacterial growth for protozoa. If, instead, you have access to scientific catalogs, then it's fairly easy simply to order proteose peptone, glucose, and agar. The peptone is a standard component of typical "nutrient agar" plates that, I believe, can be bought commercially, and the glucose may be needed as a sugar source and carbon source (?). The agar serves to harden the medium much like gelatin is supposed to. I hope this helps. Nelson Conti [a graduate student formerly from San Francisco State University with a M.A. degree in microbiology & a B. A. degree from UC Davis in Bacteriology ]
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Dear tina and others, My experience with samples you have described makes me head for the nearest exit! However, I have looked at samples such as expanded teflon, and vascular castings mounted on nothing more than carbon sticky tabs. Frequently I have clientes who do not want samples to be gold coated and consequently, charging is an issue.
I frequently examine this specimens at an extremely reduced kV and adjust the spot size and working distance to help in obtaining the best resolution. The parameters will be different for a FESEM: I am using a tungsten system. I would think a coating of colloidal carbon or silver to ground would help under any circumstances. Any thoughts? Good luck!
Ken Tiekotter
On Tue, 28 Dec 1999, Tina Carvalho wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, all- } } We have a Hitachi S-800 FESEM that is mostly used for biological } specimens. However, recently we have had a number of planetary geologists } looking at sections of meteorites mounted on epoxy resin. About the time } they started using the scope, we started having a number of problems that } suggest that we are getting some outgassing and contamination. I'm not } surprised; this happened before when looking at fish ear bones in similar } resin, despite the resin manufacturer's clain their product was completely } stable in the high vacuum and under the beam. } } My question is for those of you who routinely look at such samples: What } do you do to minimize the potential problems? Hold the samples in a } vacuum for some period of time before putting them into the scope? Paint } the exposed epoxy areas with carbon paint or similar? For the biological } samples we only require that the specimens be held over dessicant } overnight, but I suspect that more heroic measures must be taken for these } samples. } } Happy New Year to all! } } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } }
I have a multi-element standards mount which over time has deteriorated and become contaminated with salts. Three of the standards have fallen out of the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in 1982, and it appears that this company no longer exists. I am interested in finding someone to re-polish the mount and replace the lost standards. Does anyone know of such a company/person?
Thanks in advance,
Jean M. Howard Reynolds Metals Company Materials Characterization-Electron Microscopy E-mail: jmhoward-at-rmc.com Office: 804.751.2554
Dear Jean,
Plano W. Plannet GmbH is a supplier for electron microscopy. This company offers refurbishment of SEM standards. The company is situated in Germany.
Address: Ernst-Befort-Str.12 D-35578 Wetzlar
e-mail: plano-at-t-online.de, or plano-at-plano-em.com http://www.plano-em.com
You might contact them to see if they can help you with your problem.
The New York State Center for Advanced Thin Film Technology The University at Albany - State University at New York
The Center for Advanced Thin Film Technology at the University at Albany - SUNY seeks applicants for a postdoctoral position with established expertise in the area of analytical electron microscopy. Demonstrated experience in the development and application of SEM and/or TEM based analyses, including chemical analysis, is required to achieve targeted technique development goals and to support an expanding microscopy base at the Center. Experience with, or interest in, scanning probe microscopy techniques is also desirable. Candidates with a Ph.D. in applied physics, materials science and engineering, chemistry or related fields are encouraged to apply. This appointment is part of an ongoing multi-year investment in personnel and new laboratory facilities for thin film technology research areas at the Center.
The Center is a New York State sponsored high technology research and development center with a $60M state-of-the-art infrastructure, including class 1 clean rooms for back end IC manufacturing on 200 mm wafers, and advanced processing, patterning and characterization capabilities for single and multi-layered thin films. The Center is currently assembling a 200 mm wafer facility for MEMS integration. In addition, a new facility that will house a 25,000 ft2 class 1cleanroom for 300 mm wafer pilot manufacturing and workforce training is presently under construction.
Please send a letter of application including a summary of research interests, curriculum vitae, and three letters of recommendation to:
William Harris, Director of Analytical Sciences NYS Center for Advanced Thin Film Technology CESTM B110 251 Fuller Road Albany, NY 12203
The SUNY/Research Foundation is an Equal Opportunity/Affirmative Action Employer. All positions contingent upon availability of funding.
The New York State Center for Advanced Thin Film Technology The University at Albany - State University at New York
The New York State Center for Advanced Thin Film Technology at the University at Albany - SUNY is a fast growing, high technology research and development program with a mission of supporting industry and creating new jobs. This position will serve as a primary infrastructure support person for the Center's scientific and technical staff.
Job responsibilities include: performing routine maintenance and calibration of a variety of surface spectroscopic and electron microscopy instruments; performing analytical work with these instruments; assisting students with the operation of this equipment; diagnosing and repairing non-routine problems with the instrumentation; and maintaining an adequate store of spare parts and supplies.
The position requires: an associates degree in electronics, computer science or related technology and two to four years of directly relevant experience; a working knowledge of high vacuum systems; proficiency with related computer hardware/software; demonstrated ability to work in a high energy, team oriented environment; and excellent communication and analytical skills. Experience with surface analysis and/or electron microscopy instrumentation is highly desirable.
Please send a letter of application including a summary of research interests, curriculum vitae, and three letters of recommendation to:
William Harris, Director of Analytical Sciences NYS Center for Advanced Thin Film Technology CESTM B110 251 Fuller Road Albany, NY 12203
The SUNY/Research Foundation is an Equal Opportunity/Affirmative Action Employer. All positions contingent upon availability of funding.
I have a question for all of the SEM microscope labs with EDS.
What is the percent of Biological use that the EDS gets in your facility?
This is going to be my first semester teaching a Biological EDS class. The facility here sees very little, if any, biological EDS projects. It is used here as in other places I am familiar with nearly 100% non biological applications.
I would like as many responses as possible so I can give a representative summary to the students in the class. A short reply directly to me ( Geoffrey.Lloyd.Williams-at-cmich.edu or willi1gl-at-cmich.edu ) would be much appreciated. A rough % bio use, most popular bio samples (plants or animals, type of organisms . . .) and most common analysis (Quantitative, Qualitative, Dot mapping. . .).
Thank you in advance. If there is sufficient interest I would be willing to put a summary up on the list for all.
-Geoff W.
-- Geoff Williams
Electron Microscope Facility Supervisor Biology Department Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
Ken, If you have a vacuum evaporator Ken, I would try indirect carbon. Most metals will evaporate directionally only. Carbon will also evaporate indirectly (around corners) although at a reduced thickness. The advantage of this type of evaporation is the carbon will coat very convoluted surfaces which are not line of site. Another advantage is the carbon will add very little structure to your sample at high magnifications. Additionally heating of the sample can be reduced due to shading of the source from the sample. One caveat is, as you know, carbon is a very inefficient secondary producer and the carbon will not have the efficiency of gold. Indirect carbon, with a proper thickness, will prevent charging though. Just put a line of site shield between your source and sample while keeping it as small as possible. Good luck. Russ Gillmeister, Xerox
-----Original Message----- } From: Ken Tiekotter [mailto:tiekotte-at-up.edu] Sent: Wednesday, January 05, 2000 3:07 AM To: Tina Carvalho Cc: Microscopy Listserver
Dear tina and others, My experience with samples you have described makes me head for the nearest exit! However, I have looked at samples such as expanded teflon, and vascular castings mounted on nothing more than carbon sticky tabs. Frequently I have clientes who do not want samples to be gold coated and consequently, charging is an issue.
I frequently examine this specimens at an extremely reduced kV and adjust the spot size and working distance to help in obtaining the best resolution. The parameters will be different for a FESEM: I am using a tungsten system. I would think a coating of colloidal carbon or silver to ground would help under any circumstances. Any thoughts? Good luck!
Ken Tiekotter
On Tue, 28 Dec 1999, Tina Carvalho wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, all- } } We have a Hitachi S-800 FESEM that is mostly used for biological } specimens. However, recently we have had a number of planetary geologists } looking at sections of meteorites mounted on epoxy resin. About the time } they started using the scope, we started having a number of problems that } suggest that we are getting some outgassing and contamination. I'm not } surprised; this happened before when looking at fish ear bones in similar } resin, despite the resin manufacturer's clain their product was completely } stable in the high vacuum and under the beam. } } My question is for those of you who routinely look at such samples: What } do you do to minimize the potential problems? Hold the samples in a } vacuum for some period of time before putting them into the scope? Paint } the exposed epoxy areas with carbon paint or similar? For the biological } samples we only require that the specimens be held over dessicant } overnight, but I suspect that more heroic measures must be taken for these } samples. } } Happy New Year to all! } } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } }
Due to some recent high-resolution requirements in our lab, I find myself having to go back to Sputter Coating 101 (after years of just putting specimens in the coater and turning it on without a second thought!). We find ourselves in need of very thin coatings with as little structure as possible, in order to image samples down in the nanometer range.
We have a chromium coater, but often need to revisit samples days or weeks after the initial coating. The oxidation problem rears its ugly head. We intend to purchase a platinum target, but don't yet have one, so we're experimenting with our venerable Au/Pd coater.
My questions are:
1) I seem to remember a string on this listserver suggesting that lower deposition currents yield finer coating structure. Is this right? Does a low deposition current for a longer time yield a finer coating than a higher current for a shorter time? (I'm running some tests to check this, but would be very interested in others' experiences, too.)
2) Deposition current can be controlled by the initial current setting (i.e., the knob on the machine) and by the argon flow through the chamber. Is there any difference in the coating when adjusting the deposition current by either of these two ways?
3) Charts I have seen indicate that deposition current is directly proportional to coating rate. Is the same true for coating time? I.e., is a one minute coating twice as thick as a 30 sec. coating? It would seem so intuitively, but you know what they say about the word "assume".
My apologies if these are very basic questions, but, like I said, back to SC 101!
I'll be happy to summarize the responses for anyone who is interested.
Thanks!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine Bldg. University of Missouri Columbia, MO 65211 (573)882-8304 tindallr-at-missouri.edu http://www.biotech.missouri.edu/emc/
You can probably use Agar, this is commonly used to grow yeast cultures for brewing. This is a vegetable based gelatin like substance. You can buy it at some organic type stores in the bulk section (you don't need much probably 10 tbsp) it costs about $100/ounce.
I don't remember the exact proportions but I think you use something like 1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will probably be fine). You just get it al boiling for 5-10 min to sterilize it and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put it in the fridge and it should harden in 10-15 min. If not, then my proportions are off.
After it is hard, just wipe some saliva on the media and watch the green and red goo grow.
Good luck -andrew
----- Original Message ----- } From: Mike Southwell {mjsouth-at-flash.net} To: {microscopy-at-sparc5.microscopy.com} Sent: Monday, January 03, 2000 7:44 PM
Dear Dorrance, It seems to me that the etching step is redundant. The ion-milling should remove any polishing deformation and the etching will introduce surface profiling. I recall that the CZT is very soft and may require some modification to the ion beam voltage or current to reduce damage. Sounds like you are close to getting good results. At 06:34 PM 1/4/00 -0700, you wrote: } } Hi, } I've been working on thin foils of CZT and I'm looking for some suggestions } to help eliminate artifacts created by ion milling. So far I have polished } side one and etched (to remove polishing damage) with bromine-methanol and } then dimpled side two, ending with 0.1um alumina. I have been successful } obtaining a final sample thickness of about 8 to 10 microns. However, when } I put the samples into the PIPs (Gatan) to complete the thinning process I'm } seeing beam damage. I was hoping that someone might have a different } approach or suggestion that would eliminate the beam damage. } Thanks for your help. } Dorrance } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
We have always kept our osmium solutions in glass containers. However, we are in the process of evaluating our safety procedures and discussed the idea of increasing the safety of the transport of osmium from the refrigerator to the fume hood by putting the osmium solution in plastic containers. (If they are dropped, they would not break and cause the danger of a spill outside the hood.) Does anyone have experience with osmium stored in plastic? Any comments about this particular subject or any of your safety with osmium procedures are welcomed. Thanks.
Donna Wagahoff SIU School of Medicine PO Box 19627 Springfield, IL 62794-9627 217-782-0898 fax217-524-3227
This experiment is very popular in the science fair circuit; it is only slightly less popular than "What kind of music do plants like best?". Moreover (as a topic RELEVANT TO THE LIST) van Leeuwenhoek showed more than 300 years ago, BY MICROSCOPY, that the experiment as usaully performed is invalid as a test of mouthwash efficacy in situ. You might want to consult his observations on the topic.
Prepared agar media can be purchased from educational scientific supply houses such as Carolina Biologicals or Ward's, both of which maintain web sites. They both offer "instant" formulations designed for preparation of Petri dishes without autoclaving. You would want a medium capable of supporting growth of Streptococcus species, which are an important component of the oral microflora. The streptococci are somewhat fastidious in their nutritional requirements. Therefore, select something rich in organic nutrients such as amino acids. Tryptic Soy Medium would probably work best. The proteose peptone/glucose composition suggested by a previous respondent would also probably work. Lettuce or wheat grain extract would probably not give satisfactory results.If you wish to make your own medium at home from local sources, I suggest table sugar, beef bullion (more concentrated than in culinary use) and agar (often available at health food stores). A pressure cooker would help to ensure sterility during preparation. Be advised that many of the bacteria in the oral cavity are obligate anaerobes; they will not grow on any medium if it is in contact with the atmosphere.
Best wishes for the success of your daughter's work! Mike Dalbey Biology Dept. University of California Santa Cruz, CA 95064
Randy Tindall wrote: ================================================= {snip} We find ourselves in need of very thin coatings with as little structure as possible, in order to image samples down in the nanometer range.
We have a chromium coater, but often need to revisit samples days or weeks after the initial coating. The oxidation problem rears its ugly head. We intend to purchase a platinum target, but don't yet have one, so we're experimenting with our venerable Au/Pd coater.
{snip} =================================================== One (maybe the only) solution to the problem is to coat with osmium metal in the osmium plasma coater. Pt grain size can be on the same size magnitude as your nanostructures. You can get information about this coater on our website at URL http://www.2spi.com/catalog/osmi-coat.html
One does not need to worry about grain size because it is an (apparently completely) amorphous layer and since it is a precious group metal, it has the intertness of Au and Pt and will essentially last forever.
The physics of the process is explained in the US Patent #5855682 which can be clicked to from the above URL.
We would be happy to coat some demo samples for you in our demo unit at any time, just let us know.
Disclaimer: SPI Supplies distributes the line of Osmium Plasma Coaters, manufactured by Nippon Laser and Electronics Ltd.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
Randy Tindall wrote: ================================================= {snip} We find ourselves in need of very thin coatings with as little structure as possible, in order to image samples down in the nanometer range.
We have a chromium coater, but often need to revisit samples days or weeks after the initial coating. The oxidation problem rears its ugly head. We intend to purchase a platinum target, but don't yet have one, so we're experimenting with our venerable Au/Pd coater.
{snip} =================================================== One (maybe the only) solution to the problem is to coat with osmium metal in the osmium plasma coater. Pt grain size can be on the same size magnitude as your nanostructures. You can get information about this coater on our website at URL http://www.2spi.com/catalog/osmi-coat.html
One does not need to worry about grain size because it is an (apparently completely) amorphous layer and since it is a precious group metal, it has the intertness of Au and Pt and will essentially last forever.
The physics of the process is explained in the US Patent #5855682 which can be clicked to from the above URL.
We would be happy to coat some demo samples for you in our demo unit at any time, just let us know.
Disclaimer: SPI Supplies distributes the line of Osmium Plasma Coaters, manufactured by Nippon Laser and Electronics Ltd.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
Agar can be purchased from some Asian stores at much cheaper rate if you can find them. It is made into strands, so, don't expect powder form.
Ann Fook Yang EM Unit, Eastern Cereal and Oilseed Research Centre, Rm 2091, K.W. Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, Canada K1A 0C6
Phone: 613-759-1638 Fax; 613-759-1701
} } } "Andrew Cahill" {cahill-at-colorado.edu} 01/05 11:15 AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Mike,
You can probably use Agar, this is commonly used to grow yeast cultures for brewing. This is a vegetable based gelatin like substance. You can buy it at some organic type stores in the bulk section (you don't need much probably 10 tbsp) it costs about $100/ounce.
I don't remember the exact proportions but I think you use something like 1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will probably be fine). You just get it al boiling for 5-10 min to sterilize it and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put it in the fridge and it should harden in 10-15 min. If not, then my proportions are off.
After it is hard, just wipe some saliva on the media and watch the green and red goo grow.
Good luck -andrew
----- Original Message ----- } From: Mike Southwell {mjsouth-at-flash.net} To: {microscopy-at-sparc5.microscopy.com} Sent: Monday, January 03, 2000 7:44 PM
Agar can be purchased from some Asian stores at much cheaper rate if you can find them. It is made into strands, so, don't expect powder form.
Ann Fook Yang EM Unit, Eastern Cereal and Oilseed Research Centre, Rm 2091, K.W. Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, Canada K1A 0C6
Phone: 613-759-1638 Fax; 613-759-1701
} } } "Andrew Cahill" {cahill-at-colorado.edu} 01/05 11:15 AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Mike,
You can probably use Agar, this is commonly used to grow yeast cultures for brewing. This is a vegetable based gelatin like substance. You can buy it at some organic type stores in the bulk section (you don't need much probably 10 tbsp) it costs about $100/ounce.
I don't remember the exact proportions but I think you use something like 1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will probably be fine). You just get it al boiling for 5-10 min to sterilize it and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put it in the fridge and it should harden in 10-15 min. If not, then my proportions are off.
After it is hard, just wipe some saliva on the media and watch the green and red goo grow.
Good luck -andrew
----- Original Message ----- } From: Mike Southwell {mjsouth-at-flash.net} To: {microscopy-at-sparc5.microscopy.com} Sent: Monday, January 03, 2000 7:44 PM
I store my osmium in a glass bottle with plastic cap (Schott bottle - orange cap - commonly used for tissue culture work). The inside portion of the cap turns black quite rapidly but the solution is stable for months at room temperature. I store this glass bottle inside an aluminum-foiled plastic container in my fume hood at room temp. The clear plastic of this outer container gets translucent black within weeks no matter how carefully I seal the glass bottle. I think the storage of osmium in any single container (except a sealed ampule) in the refrigerator is foolish and unnecessary. Due to University regulations, we are required to store our aldehyde fixatives and other toxic chemicals inside the refrigerator in "secondary containment" containers. We keep the glass bottles of aldehydes in small plastic containers and transport them this way to the fume hood for use.
} } } } We have always kept our osmium solutions in glass containers. However, we } are in the process of evaluating our safety procedures and discussed the } idea of increasing the safety of the transport of osmium from the } refrigerator to the fume hood by putting the osmium solution in plastic } containers. (If they are dropped, they would not break and cause the danger } of a spill outside the hood.) } Does anyone have experience with osmium stored in plastic? Any comments } about this particular subject or any of your safety with osmium procedures } are welcomed. } Thanks. } } Donna Wagahoff } SIU School of Medicine } PO Box 19627 } Springfield, IL 62794-9627 } 217-782-0898 } fax217-524-3227
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
} We have always kept our osmium solutions in glass containers. However, we } are in the process of evaluating our safety procedures and discussed the } idea of increasing the safety of the transport of osmium from the } refrigerator to the fume hood by putting the osmium solution in plastic } containers. (If they are dropped, they would not break and cause the danger } of a spill outside the hood.) } Does anyone have experience with osmium stored in plastic? Any comments } about this particular subject or any of your safety with osmium procedures } are welcomed.
Dear Donna, How about putting the glass containers inside plastic containers? There are also padded and styrofoam containers which you could use for the transportation step. These can usually be made out of waste packaging material--better than sending it to a landfill. Yours, Bill Tivol
} I know this is a little off the microscopy subject, but in the interest } of encouraging scientific exploration in a young person. I hope you will } indulge me. } } I'm looking for materials for my daughter's science project. She is } testing the effectiveness of different mouthwashes in killing bacteria } found in the mouth. Her experiment matrix requires 12 petri dishes with } a bacterial growth medium. I can probably scrounge something to use as } petri dishes, but the growth medium is a problem. I'm not even sure } what it is properly called or what its makeup is. Is it something we } can easily purchase locally, or even better is there a recipe for making } a suitable substitute from ingredients found in the home? } } Any and all suggestions will be greatly appreciated. } Mike -
You're a supportive parent! I agree with you that purchasing prepared microbiological groth media doesn't have as much learning potential as starting from scratch, but it's undeniably easier. And probably cheaper. How old is she? Since you obviously don't know any microtechnique, I worry about the adequacy of her "experiment matrix". Some degree of sterile technique is required, implying the use of an alcohol lamp-sterilized wire loop.
If you want to cook your own, you'll need a pressure cooker and the instructions available in Zook et al., "The Microcosmos guide to exploring microbial space" (see the MICRO bibliography! URL below). I can send you photocopied pages, but if this is a major project you may want the book. Or you can buy prepared sterile plates (and the agarose that you'd need for home cooking) from any large biological supply house, such as Carolina Biological (800-334-5551). You may even have a medical supply source in town.
Caroline
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
Rick Felten-at-MACDERMID 01/05/2000 04:16 PM I have a couple of Ni/Au Samples that I need analyzed using AFM. The conductive samples would need to be scanned over a 10 X 10 micron region. Were are located in Waterbury Ct and the closer to us the better.
Is there a way to remove a moderate barrel distortion from an image? I have images taken with a 17 mm wide angle lens that are slightly distorted and wish to make some area measurements from them.
I have a picture of a grid of known size so it seems like there should be a way to 'stretch' the picture into shape in something like Photoshop, I just don't know where to look. Any help or ideas?
Thanks
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
The drive belt on our Reichert (Leica) Ultracut E broke last night, leaving a number of people in a state of panic. Leica does not have any of these in the US, and the Vienna group is still on holiday.
I would appreciate and forever be indebted to anyone who could quickly send me the appropriate belt, which I could either pay for (list $38.34, plus shipping) or replace when ours come in.
Mahalo! Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Nestor, The Gatan ELP option for MSA formatted files outputs the files to 5 columns serial. There are other ASCII options, but they are not MSA format. I have found that Noran does the same thing in our new system. It is not easy to parse the data for a spreadsheet when it is in that format. I would have been happy if they had done it with the two column option that is available in the standard or if they simply used a single column. Once it is in that format, it's easy to do in a spreadsheet. With multiple columns, I had to go into the file with a word processor, take out the hard returns at the end of each row, and then replace all of the commas with hard returns. Then I could open them easily in the spreadsheet. That's how I got started with the Basic program -simply to read them in and output them to a two column text with the MSA format. Then I got carried away with VB.
-Scott
} -----Original Message----- } From: Nestor J. Zaluzec [mailto:zaluzec-at-Sparc5.Microscopy.Com] } Sent: Tuesday, January 04, 2000 8:14 PM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Spectra in MSA/MSA Format How to Plot... } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } -------------------------------------------------------------- } ---------. } } } } As one of the co-authors of the MSA/MAS File format we } had a goal to make the format simple enough to be } useable in even a simple spreadsheet program. } } A number of the major manufacturer's allow you to } translate their data files into this format directly } from their application programs. To use the data } in a spreadsheet, simply specify dual column (x,y) } format for the output file and } then you can import the data files directly into } a MS Excel spreadsheet, or even better a data } graphing program like KaleidaGraph (which runs } on both Mac's & PC's). Then plot to your hearts content. } } } } Nestor } Your Friendly Neighborhood SysOp. } } }
There is a Bacterial Growth Medium Kit for grades 3-6 in the Carolina K-6 Science Catalog ( I have the Fall 1998 Catalog). It contains 2 cans of Beef Browth, 2 Boxes of Unflavored Gelatin, Multivitamins, 20 sterile Petri Dishes and 10 sterile Cotton-Tipped Applicators. It recommends the use of a pressure cooker or autoclave for "complete" sterility. It costs $26.43 per kit (1998 price) Phone: 1-800-334-5551 Most of the items are available at the supermarket. The petri dishes and sterile applicators are more difficult to get. A local college microbiology department might give a hand or even some sterile Nutrient Agar Plates.
Whenever I tried storing osmium solutions in plastics they always reacted with the plastic causing it to blacken. This took place even in Teflon altho at a much slower rate.
Why not use a glass that has been coated with plastic and rendered breakproof. I see that many chemicals (like acids) come in such reagent bottles.
Hint: maybe one of the EM vendors may know about this.
John
} We have always kept our osmium solutions in glass containers. However, we } are in the process of evaluating our safety procedures and discussed the } idea of increasing the safety of the transport of osmium from the } refrigerator to the fume hood by putting the osmium solution in plastic } containers. (If they are dropped, they would not break and cause the danger } of a spill outside the hood.) } Does anyone have experience with osmium stored in plastic? Any comments } about this particular subject or any of your safety with osmium procedures } are welcomed.
#################################################################### John J. Bozzola, Ph.D., Director Micro-Imaging and Analysis Center 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
The newer SEMs require software access to do some fundamental adjustments: magnification, high voltage, crt brightness, etc.
I am attempting to calibrate the magnification on a JEOL 5800 SEM. I am guessing this requires a sequence of keystrokes to access the computer in "service" mode. Does anyone have the keystroke sequence or know of the calibration procedure for the JEOL 5800?
We could quote on that and certainly, if it involved repolishing and re-coating only this would be worthwhile. In this case the separated standards would need to be identified, remounted and thoroughly polished. If much thickness is lost during the polishing there may be a problem with other standards. Certainly its possible but I expect that the cost will come close to our new standard blocks, complete with micro-engraving. I suggest that you check it out in our online. Disclaimer: ProSciTech supplies WDS/EDS standards.
Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Wednesday, January 05, 2000 1:48 AM, Howard, Jean M. [SMTP:JMHoward-at-rmc.com] wrote: } } Dear listers, } } I have a multi-element standards mount which over time has deteriorated and } become contaminated with salts. Three of the standards have fallen out of } the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in } 1982, and it appears that this company no longer exists. } I am interested in finding someone to re-polish the mount and replace the } lost standards. Does anyone know of such a company/person? } } Thanks in advance, } } Jean M. Howard } Reynolds Metals Company } Materials Characterization-Electron Microscopy } E-mail: jmhoward-at-rmc.com } Office: 804.751.2554 } } }
Some time ago I sent out a Kinney High Vacuum manual. There were 4 interested parties; one got the original; others, copies. I have now found a 2nd one of these antiques, and would be happy to send it to the highest bidder (i.e., free to the first to reply). The date in the front is listed as June, 1961.
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
Perhaps it is time to point out to some of the Manufacturers that they should implement the MSA/MAS standard with an option to specify the number of columns in the output file ( like the demo/test copy does). That would be in the spirit of how the format was originally designed so that "additional" programs code would not have to be written. They generally listen to customers so speak your mind!
In the mean time I'll look into also putting a version of the demo translator on-line.
(Grinning Devilishly)
Nestor Your Friendly Neighborhood SysOp
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Good Lord.......So this is what NAFTA brings us to.......Third world companies undermining real science with a New Brand of Burger King products and services? Care for an Enchilada while you wait for your SEM images?.....$5.95 please.......
Just a cynical comment that is much closer to the truth than I care to think about. -----Original Message----- } From: Paul Rennie (KIDDE) {Paul.Rennie-at-kidde-hq.com} To: microscopy-at-Sparc5.Microscopy.Com {microscopy-at-Sparc5.Microscopy.Com}
Dear All,
Having just purchased a new SEM, our old instrument, a Cambridge S200 is destined to be sent to one of our companies in Cuidad Victoria, Mexico (Although I'm trying to persuade management that it's not worth it). Although we will probably be installing the instrument ourselves, I would welcome details of any service companies in Mexico. Additionally, who sells consumables within Mexico, or is it best to purchase over the border in the USA.
Thanking you in advance.
Paul Rennie
Kidde International Research Mathisen Way Colnbrook Slough Berks SL3 0HB UK
Having just purchased a new SEM, our old instrument, a Cambridge S200 is destined to be sent to one of our companies in Cuidad Victoria, Mexico (Although I'm trying to persuade management that it's not worth it). Although we will probably be installing the instrument ourselves, I would welcome details of any service companies in Mexico. Additionally, who sells consumables within Mexico, or is it best to purchase over the border in the USA.
Thanking you in advance.
Paul Rennie
Kidde International Research Mathisen Way Colnbrook Slough Berks SL3 0HB UK
JEOL 100B - Disassembled, great source for Parts . $2,000 Cash and Carry.
PHILLIPS 201C - Will be taken out of service this month. Excellent working condition. Maintained on OEM Service Contracts since day one. Make an offer.
-- Ford M. Royer, MT(ASCP) Analytical Instruments, Ltd (Refurbished Histology, Cytology, & General Lab Equipment) 9921 13th Ave. N. Minneapolis, MN 55441-5004 800-565-1895 phone 612-929-1895 fax web site: http://www.aibltd.com
Received: from UICVM.UIC.EDU (UIC-VMNET.CC.UIC.EDU [128.248.2.49]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id KAA00322 for {zaluzec-at-sparc5.MICROSCOPY.COM} ; Thu, 6 Jan 2000 10:37:07 -0600 Received: by UICVM.UIC.EDU (IBM VM SMTP V2R4a) via spool with SMTP id 6780 ; Thu, 06 Jan 2000 10:15:16 CST Received: from UICVM (NJE origin SPAMPNS-at-UICVM) by UICVM.UIC.EDU (LMail V1.2a/1.8a) with BSMTP id 1309; Thu, 6 Jan 2000 10:15:16 -0600 Message-Id: {200001061637.KAA00322-at-Sparc5.Microscopy.Com}
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id {CF52CAJP} ; Thu, 6 Jan 2000 08:48:07 -0800 Message-ID: {EAF569A980E4D21185380090271F40EB125F35-at-EXCHANGE} {Microscopy-at-Sparc5.Microscopy.Com} , "',rfelten-at-Macdermid.com'" {,rfelten-at-Macdermid.com}
Rick,
While we are not too close to Connecticut (California to be exact), we have a great deal of experience in analyzing all types of samples with the AFM and overnight delivery services can make turnaround times very short. What information are you looking for from the samples? Please give me a call at 650-962-8767 or respond to this email so we can help you out.
-Rob
Robert J. Plano Staff Analyst, SPM Services Surface Science Labs (650)962-8767, ext. 742 http://www.cea.com http://www.surface-science.com
} -----Original Message----- } From: "rfelten-at-Macdermid.com"-at-Sparc5.Microscopy.Com } [mailto:"rfelten-at-Macdermid.com"-at-Sparc5.Microscopy.Com] } Sent: Wednesday, January 05, 2000 1:17 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: Looking for a Contract laboratory that does AFM } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html } } } -------------------------------------------------------------- } ---------. } } } } } Rick Felten-at-MACDERMID } 01/05/2000 04:16 PM } I have a couple of Ni/Au Samples that I need analyzed using AFM. The } conductive samples would need to be scanned over a 10 X 10 } micron region. } Were are located in Waterbury Ct and the closer to us the better. } } Any Help would be appreciated. } } Ric } } }
I have about 45 USED 8 inch 10 megabyte bernoulli flexible disk cartridges that I will ship to the first person that emails me at rgriffin-at-eng.uab.edu. Due to shipping charges, I will only ship to a continental U.S. address.
We have gotten rid of our computer that uses these disks and I thought someone out there that still needed them might appreciate them (since you can't buy them anymore!).
Robin Griffin Materials and Mechanical Engineering The University of Alabama at Birmingham
******************************************************* Corazon D. Bucana, Ph.D. Department of Cancer Biology U.T. M.D. Anderson Cancer Center 1515 Holcombe Blvd. Box 173 Houston, Texas 77030 Phone: (713) 792-8106 FAX: (713) 792-8747 Email:bucana-at-audumla.mdacc.tmc.edu FAX: (713) 792-8747
While they are in Florida, they deal extensively with Latin America. As for either Dr. Neddy Fookes or Dr. Barry Fookes... tell them I sent you.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 03:54 PM 1/6/00 +0000, Paul Rennie (KIDDE) wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Osmium vapors can penetrate and blacken plastics. The long term effects are uncertain. If you can find a Teflon bottle this might work.
Probably best to stay with glass bottles with Teflon colsures and do not hand carry the solution (i.e. use a lab cart for transport). Hope this is some help.
Charles Duvic, Ph.D. Chief Chemist
--
LADD RESEARCH 131 Dorset Lane Williston, VT 05495
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
This was sent to the MSA Education Committee. It seems to be a worthwhile endeavor, so I am passing it along to those on the listserver. Jay Jerome
Adam Fagen wrote:
} Dear Microscopy Community: } } The National Association of Graduate-Professional Students (NAGPS) has } recently received a grant from the Alfred P. Sloan Foundation to conduct a } survey of doctoral students on their graduate school experiences. The } survey will be completed on the Web {http://survey.nagps.org/} by current } and recent doctoral students from January - May 2000, and the results made } publicly available on the Web on a department specific basis in September. } } This effort is a follow-up to a more limited survey which occurred this } past spring, which was aimed at science and engineering doctoral students. } The aggregate results from that survey are available at } {http://www.phds.org/survey/results/} . } } The survey we are conducting is unique in at least two important ways: it } collects information on a department-specific basis, not only averaged } over entire institutions or disciplines (though discipline-level results } will also be available). So it will be possible to look at, for instance, } responses from individual biology programs, or to rank history departments } based on faculty mentoring. And it makes this data publicly available on } the Internet in Fall 2000. So we'll be opening the door about the } situation in individual departments for wider viewing by graduate } students, prospective students, faculty, administrators, etc. } } The survey is based upon best practices and covers issues in a number of } areas, including information for prospective students, curriculum breadth } and flexibility, career guidance and placement services, faculty } mentoring, time to degree, department climate, teaching, professionalism, } and overall satisfaction. In other words, the sort of best practices and } concerns outside of the reputation. The NAGPS survey itself will run from } January 18-May 1, 2000, and will be available on the Web at } {http://survey.nagps.org/} (which already has a number of resources). } } For this survey to be useful, it is vital that we reach as many current } and recent doctoral students (anyone who has been enrolled for at least } one semester in the past five years) as possible. We are hoping that we } can encourage a significant percentage of students to respond so that the } results will represent a broad range of experiences and a realistic } picture of department and institutional practices. } } In order to realize this level of participation, getting the word out is } obviously very important. One of the publicity strategies we're employing } is trying to reach current and recent doctoral students through the major } professional societies and organizations, such as the MSA. (Other } strategies include messages to relevant e-mail listservs, coverage in } campus and national media, and working through graduate student } organizations, department chairs and graduate deans, other organizations, } etc.) } } Since the MSA reaches so many current and recent graduate students, it is } certainly one of the organizations of prime importance for getting the } word out. We have thought of a few strategies for spreading the word } among your membership (and would welcome additional ideas and } suggestions): (1) distribution on e-mail listservs that reach a high } number of graduate students and recent graduates, those who have left } their programs, etc.; (2) notice in newsletters and other publications } that current and former students might see; and (3) publicity at meetings } and conferences. We are happy to provide whatever resources and materials } that would facilitate distribution (e.g., flyers, letters, posters, etc.). } We would certainly appreciate any insight you have on publicity within } (and outside of) the MSA. } } As a bit of background on our organization, NAGPS represents nearly } 900,000 graduate and professional students on 150 member campuses and is } dedicated to improving the quality of graduate and professional student } life and education by actively promoting the interests and welfare of } graduate- and professional-degree-seeking students. } } Of course, I'd be happy to answer any questions or provide any more info. } Thanks for your assistance in this important effort! } } --Adam Fagen } } Adam Fagen, Chair } Ad Hoc Committee on Faculty-Student Relations } National Association of Graduate-Professional Students (NAGPS) } } NAGPS Web: http://www.nagps.org/ } The National Doctoral Program Survey: http://survey.nagps.org/ } } Adam Fagen \ afagen-at-fas.harvard.edu } Molecular Biology/Education / http://www.fas.harvard.edu/~afagen/ } Harvard University GSAS \ http://mazur-www.harvard.edu/
-- Jay ---------------------------------------------- - AKA: W. Gray Jerome, Ph.D. - - Department of Pathology - - Wake Forest University School of Medicine - - Winston-Salem, NC 27157-1092 - - Ph: 336-716-4972, 336-716-2675 - - Fax: 336-716-6174 - - E-mail: jjerome-at-wfubmc.edu - ----------------------------------------------
Can anyone recommend image montaging software? That is, software that assists you in compositing several images into one larger image. I am familiar with the offerings from GATAN for their Digital Micrograph and QuickStitch from Enroute Software. Also, can anyone offer an evaluation of the QuickStitch software?
TIA
Rick A. Harris, Director Microscopy and Imaging Facility Section of Molecular and Cellular Biology 1241 Life Sciences Addition University of California Davis, CA 530 752 2914 530 754 7536 fax http://katie.ucdavis.edu raharris-at-ucdavis.edu
Plastic is oxidized and so part of the osmium is exhausted in the vials. Even when frozen osmium diffuses through plastic containers. So you may save a rare breakage, but you are certain to have osmium in your refrigerator. It's just a bad idea to pack osmium in plastic. You could use a secondary container, be it plastic or glass, to increase overall safety. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Thursday, January 06, 2000 3:08 AM, Donna Wagahoff [SMTP:DWAGAHOFF-at-siumed.edu] wrote: } } We have always kept our osmium solutions in glass containers. However, we } are in the process of evaluating our safety procedures and discussed the } idea of increasing the safety of the transport of osmium from the } refrigerator to the fume hood by putting the osmium solution in plastic } containers. (If they are dropped, they would not break and cause the danger } of a spill outside the hood.) } Does anyone have experience with osmium stored in plastic? Any comments } about this particular subject or any of your safety with osmium procedures } are welcomed. } Thanks. } } Donna Wagahoff } SIU School of Medicine } PO Box 19627 } Springfield, IL 62794-9627 } 217-782-0898 } fax217-524-3227
I would not recommend storing osmium in ordinary laboratory plastics containers used on their own. Osmium penetrates polyolefin plastics (PE, PP) to some depth, and reacts with them, so containers made from these plastics or of polystyrene or polycarbonate with polyolefin closures cannot be recommended. Mostly, osmium is supplied in some protective packaging, but if you want to improve security still further I would consider placing the glass ampoules inside a polyethylene or polypropylene tube. That would give considerable shock-protection, and if the ampoule did break would protect against leakage, but only for the short period required to get it to a fume cupboard. The same principle can be applied to osmium solutions prepared in glass bottles - enclosure in an outer polyethylene bottle will give shock-protection and temporary containment. It will also indicate by its blackening how much leakage is occurring from your supposedly closed glass container. Speaking of which, we have a rule that osmium solutions are NEVER stored in the laboratory refrigerator. Why? because even when greatest care is taken, osmium blackening of the fridge walls and other objects in the fridge takes place, indicating that vapour is escaping into the fridge atmosphere. If you must store osmium at low temperatures, I recommend you consider installing a compact refrigerator in your fume hood or in a fan-ventilated cupboard.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We have always kept our osmium solutions in glass containers. However, we } are in the process of evaluating our safety procedures and discussed the } idea of increasing the safety of the transport of osmium from the } refrigerator to the fume hood by putting the osmium solution in plastic } containers. (If they are dropped, they would not break and cause the danger } of a spill outside the hood.) } Does anyone have experience with osmium stored in plastic? Any comments } about this particular subject or any of your safety with osmium procedures } are welcomed. } Thanks. } } Donna Wagahoff } SIU School of Medicine } PO Box 19627 } Springfield, IL 62794-9627 } 217-782-0898 } fax217-524-3227 }
===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk =====================================================================
I just solved (with the help of some of you) my last problem with the carbon extraction replicas but the next specimen causing difficulties is on my desk...
I got a small semiconducor specimen with a layered structure that should be investigated. If I say small I mean it: it has a shape like a cube with a side length of about 250um. And now comes the real difficulty: We need to cut this one precious specimen into several slices in order to allow other investigations with other analytical methods. In fact we have five specimens to try with, but they are not completely identical to the one we have finally to investigate.
We are equipped with everything we need to do a TEM preparation of bulk materials and also of interfaces as long as the specimens are large enough (saw, disk cutter, dimpler, ion etcher, mounting tools, ...) but I don't see how I could be successful with such a small specimen.
Any ideas?
Petra
-------------------------------------------------------------- Dr. Petra Wahlbring Centre de Recherche Public - Gabriel Lippmann Laboratoire d'Analyse des Materiaux (LAM) 162a, av. de la Faiencerie L-1511 Luxembourg tel. +352-466644-402 fax +352-466644-400 e-mail: petra.wahlbring-at-crpgl.lu Visit our WWW site! http://www.crpgl.lu/~wahlbrin
I just solved (with the help of some of you) my last problem with the carbon extraction replicas but the next specimen causing difficulties is on my desk...
I got a small semiconducor specimen with a layered structure that should be investigated. If I say small I mean it: it has a shape like a cube with a side length of about 250um. And now comes the real difficulty: We need to cut this one precious specimen into several slices in order to allow other investigations with other analytical methods. In fact we have five specimens to try with, but they are not completely identical to the one we have finally to investigate.
We are equipped with everything we need to do a TEM preparation of bulk materials and also of interfaces as long as the specimens are large enough (saw, disk cutter, dimpler, ion etcher, mounting tools, ...) but I don't see how I could be successful with such a small specimen.
Any ideas?
Petra
-------------------------------------------------------------- Dr. Petra Wahlbring Centre de Recherche Public - Gabriel Lippmann Laboratoire d'Analyse des Materiaux (LAM) 162a, av. de la Faiencerie L-1511 Luxembourg tel. +352-466644-402 fax +352-466644-400 e-mail: petra.wahlbring-at-crpgl.lu Visit our WWW site! http://www.crpgl.lu/~wahlbrin
I think I can make several suggestions and point you toward several references that may help.
First, can you or have you tried dipping your ion milled (to perforation) samples into your bromine/methanol etch as the final step in your procedure? Yu et al (J.of Elec. Micro.Tech./18/315-324/1991) dipped their perforated samples in 0.5% Br2/Ch3OH for 1or 2 seconds followed by 20 seconds in HF/HCL of volume ratio 1:1. This technique worked well for them.
As you may know, reducing the energy during the final step(s) of ion milling can reduce the severity and/or the number of artifacts produced. If you have access to a mill that allows milling in the 100eV to 1keV range you may want to try this as the final step in your milling procedure.
The following techniques have also been used to prepare type II-VI compound semiconductors:
(1) Reactive ion milling. This can be done 2 different ways. In one method Iodine gas is used instead of Argon. The iodine gas is ionized to form I+, which is then used for milling (Cullis and Chew/ MRS symposium proceedings/ 115/ 3-14/1988). In the second method, Iodine gas is introduced into the Atmosphere of the milling chamber during Ar+ milling (Pecz and Barna /Vacuum/45/1-3/1994). The authors have applied these techniques to type II-VI compound semiconductors of CdTe, ZnS, and ZnSe in addition to other compound semiconductors. CAUTION must be used when introducing iodine gas into your ion mill. The gas is very corrosive and may damage mill parts and vacuum systems. I recommend that you check with the manufacturer of your ion mill before proceeding. (2) Chemomechanical polishing. Sabinina and Gutakovsky (Ultramicroscopy/45/411-415/1992) eliminated the need for ion milling completely by using this technique. They prepare samples of HgCdTe using a bromine-methanol etchant in a technique that is very similar to dimpling using padded tools.
Are you familiar with the Small Angle Cleavage Technique (SACT)? This technique may or may not work for your materials. I am not aware of any references for preparing II-VI materials using SACT but that certainly does not imply that it cant be done. I have used this technique to prepare samples of thin films on silicon substrates. It is quick and relatively easy to learn. The technique was developed by John McCaffrey and a good step by step procedure is given by Walck and McCaffrey (MRS symposium proceedings/480/149-171/1997).
The references listed above are meant to be a starting point and are by no means a complete listing.
Hope this helps.
E. Windsor
Eric Windsor Physical Scientist NIST 100 Bureau Dr. Stop 8371 Gaithersburg, MD 20899-8371 (301) 975-3930 fax: (301) 417-1321 eric.windsor-at-nist.gov
Dorrance McClean originally wrote:
Hi, I've been working on thin foils of CZT and I'm looking for some suggestions to help eliminate artifacts created by ion milling. So far I have polished side one and etched (to remove polishing damage) with bromine-methanol and then dimpled side two, ending with 0.1um alumina. I have been successful obtaining a final sample thickness of about 8 to 10 microns. However, when I put the samples into the PIPs (Gatan) to complete the thinning process I'm seeing beam damage. I was hoping that someone might have a different approach or suggestion that would eliminate the beam damage. Thanks for your help. Dorrance
Soft Imaging Systems has a program called analySIS that has a montage module called MIA. Have a look at their web site at: www.soft-imaging.de
David Rohde NORAN Instruments
-----Original Message----- } From: Rick Harris [mailto:raharris-at-ucdavis.edu] Sent: Thursday, January 06, 2000 6:47 PM To: microscopy-at-sparc5.microscopy.com
Can anyone recommend image montaging software? That is, software that assists you in compositing several images into one larger image. I am familiar with the offerings from GATAN for their Digital Micrograph and QuickStitch from Enroute Software. Also, can anyone offer an evaluation of the QuickStitch software?
TIA
Rick A. Harris, Director Microscopy and Imaging Facility Section of Molecular and Cellular Biology 1241 Life Sciences Addition University of California Davis, CA 530 752 2914 530 754 7536 fax http://katie.ucdavis.edu raharris-at-ucdavis.edu
Dear Subscribers, I would be interested in hearing from anyone who has experience in in situ hybridization to mRNA. I am working with immature embryos of Arabidopsis and would like to recieve replies and comments to the following questions:
1. For embedding, Paraffin is mostly used. Why not use LR-White (London Resin)? or BMM (Butylmethylmethacrylate)? or another acrylic material? These hydrophilic materials should be easier to remove, or is it necessary to remove them for mRNA exposure? If it is necessary, what is best for removing them? Perhaps acetone?
2. About the probe itself, obviously RNA is best. Has anyone tried DNA oligos (~20 nucleotides) for less abundant mRNAs?
3. Has anyone used tailed oligos?
4. Is DIG significantly better to use than biotin labelled probes?
Many thanks to anyone who is prepared to take the time to help reveal the unknowns of plant embryogenesis. Maura ____________________ Dr. Maura C. Cannon Dept. Biochemistry & Molecular Biology Lederle Graduate Research Center University of Massachusetts Amherst, MA 01003
Ron Anderson has a technique that might do the trick for you. He took an ultrasonic drill and made a small cavity in a piece of Si at the surface. The drill was solid spherical end. He then epoxied his small sample into the hole. You might want to mount it on another piece of Si first and then put it in the hole. He then used the Tripod Polishing technique to examine it. The benefit is that he has the Si to gauge the thickness of the sample. I though that it was pretty slick.
Your other option and the one most easy to do if you have access to an instrument is to have the sample FIB'd.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Petra Wahlbring [mailto:wahlbrin-at-crpgl.lu] } Sent: Friday, January 07, 2000 8:17 AM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Mat: Cutting of small semiconductor specimen? } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } -------------------------------------------------------------- } ---------. } } } } A happy New Year to everybody! } } I just solved (with the help of some of you) my last problem with the } carbon extraction replicas but the next specimen causing } difficulties is on } my desk... } } I got a small semiconducor specimen with a layered structure } that should be } investigated. If I say small I mean it: it has a shape like a } cube with a } side length of about 250um. And now comes the real } difficulty: We need to } cut this one precious specimen into several slices in order } to allow other } investigations with other analytical methods. In fact we have five } specimens to try with, but they are not completely identical } to the one we } have finally to investigate. } } We are equipped with everything we need to do a TEM } preparation of bulk } materials and also of interfaces as long as the specimens are } large enough } (saw, disk cutter, dimpler, ion etcher, mounting tools, ...) } but I don't } see how I could be successful with such a small specimen. } } Any ideas? } } Petra } } } -------------------------------------------------------------- } Dr. Petra Wahlbring } Centre de Recherche Public - Gabriel Lippmann } Laboratoire d'Analyse des Materiaux (LAM) } 162a, av. de la Faiencerie L-1511 Luxembourg } tel. +352-466644-402 fax +352-466644-400 } e-mail: petra.wahlbring-at-crpgl.lu } Visit our WWW site! http://www.crpgl.lu/~wahlbrin }
Every plastic container we have seen turns black and some seals break down. Our current method is similar to Bill Trivol's. We use a 25ml Pyrex media bottle with the orange plastic lid (it does not seem affected). This is placed in a spare metal can (that the ampules of osmium crystals were shipped in) with paper padding. This new bottle (with parafilm around cap) also solved our osmium fumes in the refrig. problem. Everything is small enoug to transport from refrig. to hood.
Speaking of which, we have a rule that osmium solutions are NEVER stored in the laboratory refrigerator. Why? because even when greatest care is taken, osmium blackening of the fridge walls and other objects in the fridge takes place, indicating that vapour is escaping into the fridge atmosphere. If you must store osmium at low temperatures, I recommend you consider installing a compact refrigerator in your fume hood or in a fan-ventilated cupboard.
With all due respect Chris for over 20 years we have used our lab refrigerator to store Osmium with no blackening of the plastic walls. A 25ml 4% solution of Osmium is put into a 60 ml glass stopper bottle with the stopper tightly wrapped in parafilm. Next, that bottle is placed in a waxed 6" long x 3" diameter screw capped cardboard mailing tube. We prepare the waxed tubes by pouring hot paraffin inside and rotated quickly and evenly until the entire surface area is well coated. We also coat the inside of the metal screw cap but not the outside of the tube. Mailing tubes are available from the local shipping supply house and our waxed containers have lasted decades. Regards Jim
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility U-2131 Storrs, CT 06269-2131 bsgphy3-at-uconnvm.uconn.edu 860 486-2914 voice 860 486-1936 fax
We have been using teflon bottles for many yeas with success. We got them from Fisher Sci
At 09:30 AM 1/7/00 -0600, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Gregory W. Erdos, Ph.D. Ph. 352-392-1295 Assistant Director, Biotechnology Program PO Box 110580 Fax: 352-846-0251 University of Florida Gainesville, FL 32611
} We have always kept our osmium solutions in glass containers. However, we } are in the process of evaluating our safety procedures and discussed the } idea of increasing the safety of the transport of osmium from the } refrigerator to the fume hood by putting the osmium solution in plastic } containers. (If they are dropped, they would not break and cause the danger } of a spill outside the hood.) } Does anyone have experience with osmium stored in plastic? Any comments } about this particular subject or any of your safety with osmium procedures } are welcomed.
Dear Donna,
In my point of view polypropylene and polyethylene are compatible with osmium tetroxide solutions. Only problem - to hold that nasty stuff inside the vial. Some plastics may partially be penetrable by osmium tetroxide.
I had some limited experience to store aqueous osmium tetroxide solutions in the polypropylene (?) NUNC 2 or 5 ml cryo-vials. I aliquot solution into that vials and store it at -40oC. For extra protection I stored cryo-vials in the 15 ml cell-culture tubes. Cryo-vial's seal may penetrate by osmium tetroxide vapors a little bit but second tube provides complete protection. 2-4% aqueous osmium tetroxide solutions (no buffer, please) are stable for at least 1-2 month in the dark at -20 - 40oC in that plastic containers.
Good luck.
Sergey.
_________________________________
Sergey Ryazantsev Ph. D. UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
There is a researcher at our Med. School who wants to do SEM on biofilms
on ear biopsies. He says the biopsies will be approximately 1mmX1mm. I proposed a standard glut.-osmium fix, Et-oh dehydration, CPD, mount, sput coat protocol. Is this sufficient? Do we need to affix the biopsies
to a substrate first? I would appreciate any suggestions. If anybody has reprints that contain a good protocol, I would certainly appreciate getting a copy. My address is in the footer and my FAX is 405-325-7619. Thanks for your help.
Bill Chissoe
-- ============================================================= Bill Chissoe III Electron Microscopist University of Oklahoma 770 Van Vleet Oval Norman, Ok. 73019 E-mail: wchiss-at-ou.edu Ph. (405)325-4391 =============================================================
A user of our facility wishes to do some special vacuum evaporation processes. He is looking for ceramic coated tungsten boats for the purpose, but has not been able to locate a source. Any help would be appreciated.
Replies offline would be welcome. Please reply to the list only if you have generally useful information.
Thanks,
John Chandler Colorado State University chandler-at-lamar.colostate.edu
For years, I have been using my diode GW BSE system with high beam currents and high gain (contrast) to yield electron channeling contrast on polished specimens.
FWIW: Contrary to some reports I have read, lower kV works better than higher. Since it is imperative to use high beam currents at the required resolution, I wonder if difficulty in achieving sufficient current at lower potentials may have been influencing opinions.
I have also been a very good customer for GW Electronics. When the detector is new, I generally have no problem, but as it ages, the same operating conditions will produce artifacts. A new detector every year or so is a bit expensive, but fixes the problem.
I now have a new detector (and SEM :) which produces this artifact and am now trying to find the reason.
The artifact can be described as video brightness overshoot from black to white when the black is strongly saturated. It occurs when the (very high gain) BSE signal goes from saturated black to some median gray. A low-Z inclusion or deep pore in the field of view will produce this artifact. When the beam leaves the inclusion/pore, it overshoots to white then settles back to the appropriate level. The effect is a black pore with a white "comet tail" pointing in the sweep direction. This problem does not manifest itself when using less extreme operating conditions.
I certainly cannot exclude the possibility that the detector is not the direct cause. The amount of signal gain (contrast) is not calibrated (nor have I paid much attention to the position of the adjustment) . If the detector simply loses some sensitivity, I would make up the difference in amplifier gain and not realize I was not at exactly the same operating conditions. ...Thus, the artifact could be amplifier gain related rather than simply a detector problem.
Paul Rennie wrote: =============================================== Having just purchased a new SEM, our old instrument, a Cambridge S200 is destined to be sent to one of our companies in Cuidad Victoria, Mexico (Although I'm trying to persuade management that it's not worth it). Although we will probably be installing the instrument ourselves, I would welcome details of any service companies in Mexico. Additionally, who sells consumables within Mexico, or is it best to purchase over the border in the USA. ================================================ With regard to the method of purchasing consumables from within Mexico, the situation is entirely analogous to that that exists in the UK vis a vis purchasing items from the USA directly or via a distributor in the UK. It is a matter of institutional policy and also, personal preference.
The main manufacturers of consumables all have local distributors in Mexico and those firms can be found on the websites of those respective firms. On the other hand, with the implimentation of NAFTA, making a shipment to a point in Mexico from the USA is not really all that different from making a shipment to some other point in the USA. So again it is a matter of institutional policy and personal preference.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
I have been trying for some time to find any information about a Vickers Instrument Company microscope. I had never heard of the Company but the scope looked interesting. I have searched hundreds of websites, posted messages on newsgroups and also on Microscopy, UK. So far I've had only two responses. The sum total of what I have learned is that the company was in York and that they closed in 1989. Cooke, Troughton and Simms were taken over by Vickers
The rather lengthy description of the scope follows:
Head: Binocular configuration, adjustable for interpupillary distance and dioptric differences. Interpupillary adjustment range, 50 to 72mm. Eyepieces(2): 10X, DIN standard. Objectives(4): Vickers microplan, Planachromatic objectives. Include 5X (0.15 N.A.), 10X (0.25 N.A.), 20X (0.50 N.A. spring loaded), 40X (0.65 N.A. spring-loaded), DIN standard objectives. All are parfocal, parcentered, and coated to resist reflection. Stage: Precision-machined mechanical stage with oversized, low-position, coaxial control knobs. Chemical-resistant finish with glass insert. This stage is exceptionally smooth in movement with a 3.25 x 2.75-inch range of motion. Focus Control: Low-position, coaxial, coarse/fine controls. . Condenser: Abbe condenser, fitted with an iris diaphragm. Illumination: Diascopic lower illumination features variable-intensity quartz halogen lamp ( 12V, 100 watt ) with metered solid-state control as well as field iris, condenser and centering adjustments. Episcopic illumination (30-watt lamp) is also of variable intensity via an independent control on front of the microscope base. Upper illuminator housing fitted with iris and condenser controls. As shown in the photos, the microscope can be separated from the 100-watt illuminator base Finish chemical-resistant paint with "hammertone" finish.
I have included some pictures that I have posted to help with the identification:
I am trying to find any information about the company and about the scope itself. I understand that this is rather difficult (impossible?) because of the practice of the company not to use serial numbers. I would especially like to be able to aquire a copy of the owners manual and a copy of the Vickers catalog.
When I began to attempt to collect information about the Vickers I never guessed that I would run into a blank wall. If you could assist me in any way at all it would be greatly appreciated.
I am looking for a simple method to indicate the presence of lipids /oils in plant tissue. Specifically palm fruit tissues. Ideally, if there is a procedure that I can used on fruits fixed in FAA that would be the best. I have tried Sudan Black B without success. Thank you in advance for your ideas or suggestions. Please reply via email to : mchapin-at-ntbg.org
The item you are looking for should be available from R.D. Matis. The following contact information is from www.vacuum.org. There are several vendor listed in the subsection for filaments if you care to browse. I have no financial interest in R.D. Matis.
R.D. Mathis Company 2840 Gundry Avenue Long Beach, CA 90806 Phone: 562-426-7049 FAX: 562-595-0907
Description
Specializes in the manufacture of hi-vacuum evaporation sources. We offer a comprehensive selection of tungsten, molybdenum and tantalum sources as well as custom fabrication to meet your specific needs. Display will be a variety of evaporation sources along with one of our "LV Series" low voltage high current power supplies and our "GP 100" inert gas purifier to compliment your evaporation process.
Product Categories Filaments
Good luck
Jim Campbell
James Campbell 36 Van Drive Bordentown, NJ 08505 Tel: 609-298-9206 Fax: 609-278-6969 e-mail campbell36-at-aol.com
In a message dated 1/7/00 9:56:09 PM Eastern Standard Time, chandler-at-lamar.ColoState.EDU writes:
} Subj: Vac Evap: Special needs request } Date: 1/7/00 9:56:09 PM Eastern Standard Time } From: chandler-at-lamar.ColoState.EDU (John Chandler) } To: Microscopy-at-Sparc5.Microscopy.Com } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } A user of our facility wishes to do some special vacuum evaporation } processes. He is looking for ceramic coated tungsten boats for the } purpose, but has not been able to locate a source. Any help would be } appreciated. } } Replies offline would be welcome. Please reply to the list only if you } have generally useful information. } } Thanks, } } John Chandler } Colorado State University } chandler-at-lamar.colostate.edu
James Campbell 36 Van Drive Bordentown, NJ 08505 Tel: 609-298-9206 Fax: 609-278-6969 e-mail campbell36-at-aol.com
Dana: This may not help, but I have a book I purchased about 1970 that was published by Vickers entitled: The Polarizing Microscope by A.F. Hallimond. It is an excellent work and has many pictures of the Vickers line of polarizing scopes and accessories from the late 60s. They did buy out Cooke, Troughton and Simms and marketed a number of innovative and competitively priced (cheaper than Leitz and Zeiss) scopes. Dr Hallimond was one of their consultants on design. I am sure you can find the book in a large University Geology department library ( I hope). Or try interlibrary loan. It is still an excellent and definitive reference for polarizing light microscopes and their use.
Michael L. Boucher Sr. mboucher-at-isd.net http://www.isd.net/mboucher
I am trying to quantify the alloy amount of Al/Si. The standard alloy ratio is 1-3 wt % Si/Al. However, with Z of each only 1 unit apart, and low Z at that, EDAX and AES cannot detect the Si. My next attempt is to try dynamic SIMS and then time of flight SIMS.
The specimen is a microcircuit die. I am analyzing the bonding pad metalization.
Has anyone done this sort of thing before and had success? If so, how did you do it?
Vickers was a well known British manufacturer of microscopes. I have had the privilege of using some of their more interesting measuring equipment. Several contacts come to mind, most of them from the UK: Clive Cowan, Micro Instruments in Oxford (UK) 199-388-9616 and Gerard Turner, who is a curator in microscopy at the Museum of Science at Oxford University, Dr. Savile Bradbury, retired from Oxford (but could probably be reached by letter there; also, if you are interested, I can probably get a more recent address), and Dr. Cecile Fox at Molecular Histology in Gaithersburg (301-216-1564). Cecile put together major microscopy exhibits for both the Smithsonian and the Am. Mus. of Natural History in the mid- 1980's. Please give my regards to them (Gerard may only remember me as an RMS student from the distant past) and best of luck on your search.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 12:41 PM 1/8/00 EST, Lugosi1936-at-aol.com"-at-Sparc5.Microscopy.Com wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Do you have an evaporator in the lab? Simultaneous evaporation of Pt (wire) and carbon gives a very fine and permanent coating. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Thursday, January 06, 2000 2:15 AM, Tindall, Randy D. [SMTP:TindallR-at-missouri.edu] wrote: } } } Hi, } } Due to some recent high-resolution requirements in our lab, I find myself } having to go back to Sputter Coating 101 (after years of just putting } specimens in the coater and turning it on without a second thought!). We } find ourselves in need of very thin coatings with as little structure as } possible, in order to image samples down in the nanometer range. } } We have a chromium coater, but often need to revisit samples days or weeks } after the initial coating. The oxidation problem rears its ugly head. We } intend to purchase a platinum target, but don't yet have one, so we're } experimenting with our venerable Au/Pd coater. } } My questions are: } } 1) I seem to remember a string on this listserver suggesting that lower } deposition currents yield finer coating structure. Is this right? Does a } low deposition current for a longer time yield a finer coating than a higher } current for a shorter time? (I'm running some tests to check this, but } would be very interested in others' experiences, too.) } } 2) Deposition current can be controlled by the initial current setting } (i.e., the knob on the machine) and by the argon flow through the chamber. } Is there any difference in the coating when adjusting the deposition current } by either of these two ways? } } 3) Charts I have seen indicate that deposition current is directly } proportional to coating rate. Is the same true for coating time? I.e., is a } one minute coating twice as thick as a 30 sec. coating? It would seem so } intuitively, but you know what they say about the word "assume". } } My apologies if these are very basic questions, but, like I said, back to SC } 101! } } I'll be happy to summarize the responses for anyone who is interested. } } Thanks! } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility } W122 Veterinary Medicine Bldg. } University of Missouri } Columbia, MO 65211 } (573)882-8304 } tindallr-at-missouri.edu } http://www.biotech.missouri.edu/emc/ }
I'm not 100% sure but I thought that the Microscience Division of Bio-Rad bought Vickers some years ago. You might want to give them a shot.
best regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
Don't go around saying the world owes you a living; the world owes you nothing; it was here first. Mark Twain [Samuel Langhornne Clemens] (1835-1910)
-- Begin original message -- } -----------------------------------------------------------------------. } } } } I have been trying for some time to find any information about a Vickers } Instrument Company microscope. I had never heard of the Company but the scope } looked interesting. I have searched hundreds of websites, posted messages on } newsgroups and also on Microscopy, UK. So far I've had only two responses. } The sum total of what I have learned is that the company was in York and that } they closed in 1989. Cooke, Troughton and Simms were taken over by Vickers } } The rather lengthy description of the scope follows: } } Head: Binocular configuration, adjustable for interpupillary distance and } dioptric differences. Interpupillary adjustment range, 50 to 72mm. } Eyepieces(2): 10X, DIN standard. Objectives(4): Vickers microplan, } Planachromatic objectives. Include 5X (0.15 N.A.), 10X (0.25 N.A.), 20X (0.50 } N.A. spring loaded), 40X (0.65 N.A. spring-loaded), DIN standard objectives. } All are parfocal, parcentered, and coated to resist reflection. Stage: } Precision-machined mechanical stage with oversized, low-position, coaxial } control knobs. Chemical-resistant finish with glass insert. This stage is } exceptionally smooth in movement with a 3.25 x 2.75-inch range of motion. } Focus Control: Low-position, coaxial, coarse/fine controls. . Condenser: Abbe } condenser, fitted with an iris diaphragm. Illumination: Diascopic lower } illumination features variable-intensity quartz halogen lamp ( 12V, 100 watt } ) with metered solid-state control as well as field iris, condenser and } centering adjustments. Episcopic illumination (30-watt lamp) is also of } variable intensity via an independent control on front of the microscope } base. Upper illuminator housing fitted with iris and condenser controls. As } shown in the photos, the microscope can be separated from the 100-watt } illuminator base Finish chemical-resistant paint with "hammertone" finish. } } I have included some pictures that I have posted to help with the } identification: } } {A HREF="http://members.aol.com/lugosi1936/vic1a.jpg"} http://members.aol.com/l } ugosi1936/vic1a.jpg {/A} } {A HREF="http://members.aol.com/lugosi1936/vic2.jpg"} http://members.aol.com/lu } gosi1936/vic2.jpg {/A} } {A HREF="http://members.aol.com/lugosi1936/vic3.jpg"} http://members.aol.com/lu } gosi1936/vic3.jpg {/A} } } I am trying to find any information about the company and about the scope } itself. I understand that this is rather difficult (impossible?) because of } the practice of the company not to use serial numbers. I would especially } like to be able to aquire a copy of the owners manual and a copy of the } Vickers catalog. } } When I began to attempt to collect information about the Vickers I never } guessed that I would run into a blank wall. If you could assist me in any way } at all it would be greatly appreciated. } } Best regards, } Dana } }
first, I would like to wish you all the best for the new year. Now my question: Does anyone know the shelf-life/usability of LR-Gold stored in a fridge? Is it that delicate as LR-White (Meanwhile I donŦt use LR-W older than half a year). My bottle which was not opened many times, could be roundabout three years old.
Thanks a lot, Michael
Michael Reiner Department of Anatomy I University of Cologne Germany michael.reiner-at-smail.uni-koeln.de
Please send me (if possible) a brief explanation of gamma correction in image analysis and a good reference source (book, papers, etc) to get the details... Thanks
Dear Randy, There was a good series of articles in the Scanning Electron Microscopy, 1980, volume I (pp. 143--218), that examined a number of thin-film deposition methods and measured the films for feature size, mostly using TEM. They found that lower kV made for finer films, using Mo, W or Ta made a more featureless coating, Pt was a little finer than Au/Pd and that lowering the temperature of the substrate also made the films finer. In my own experience I found that, on smoother specimens, even a two second coating would sometimes be enough to stop charging. Try very short times and turn the specimen and coat again for a very short time, if the first one isn't enough. I used 700V, if your coater can change voltage. If you can find the Scanning Electron Microscopy articles, they have other good suggestions. At 10:15 AM 1/5/00 -0600, you wrote:
} Hi, } } Due to some recent high-resolution requirements in our lab, I find myself } having to go back to Sputter Coating 101 (after years of just putting } specimens in the coater and turning it on without a second thought!). We } find ourselves in need of very thin coatings with as little structure as } possible, in order to image samples down in the nanometer range. } } We have a chromium coater, but often need to revisit samples days or weeks } after the initial coating. The oxidation problem rears its ugly head. We } intend to purchase a platinum target, but don't yet have one, so we're } experimenting with our venerable Au/Pd coater. } } My questions are: } } 1) I seem to remember a string on this listserver suggesting that lower } deposition currents yield finer coating structure. Is this right? Does a } low deposition current for a longer time yield a finer coating than a higher } current for a shorter time? (I'm running some tests to check this, but } would be very interested in others' experiences, too.) } } 2) Deposition current can be controlled by the initial current setting } (i.e., the knob on the machine) and by the argon flow through the chamber. } Is there any difference in the coating when adjusting the deposition current } by either of these two ways? } } 3) Charts I have seen indicate that deposition current is directly } proportional to coating rate. Is the same true for coating time? I.e., is a } one minute coating twice as thick as a 30 sec. coating? It would seem so } intuitively, but you know what they say about the word "assume". } } My apologies if these are very basic questions, but, like I said, back to SC } 101! } } I'll be happy to summarize the responses for anyone who is interested. } } Thanks! } } Randy } Best of luck, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Is anyone willing to do some contract cryoSEM or TEM in the Chicagoland area? I have a primary emulsion with particle size less than 1.0 micrometers. 2 samples. Please contact me via the server. Joanne Crudele-Unilever-Rolling Meadows Il. Give a price estimate per sample.
You are corrrect in that the time constant of diode BSE detectors is relatively slow. Unfortunatly, I am already sweeping about as slowly as possible to minimize the effect and help the signal to noise ratio.
Woody, Is the problem independent of scan speed? Slower scan speeds often overcome distortion using solid state BEI detectors.
At 05:41 PM 1/7/00 -0600, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
id AA30574; Mon, 10 Jan 2000 19:01:49 +0800 Message-Id: {200001101101.AA30574-at-aphy.iphy.ac.cn}
Hi, Microscopist,
International Kunming Symposium on Microscopy (IKSM) will be held on July 2-5, 2000, in Kunming, one of the most attractive tourist destinations in China.
Call-for-paper circular and pre-registration form for International Kunming Symposium on Microscopy (IKSM) are available on request by email. For more information, pls visit http://www.iphy.ac.cn/microsc/IKSM.html .
Rick Felten-at-MACDERMID 01/10/2000 04:20 PM Whenever I wanted to merge images I used corel draw 8. I inserted the images where I wanted them and exported the entire image as a tiff. Seem to work ok. Not sure if it is the most efficient way. In fac, it is the only thing that I liked about corel draw over MS publisher.
To the Board, We have a problem with spotty,black,amorphous artifacts on our SEM samples visible at 200X and up. The problem seems to arise only with one sample type, epoxy resin casts made with amine-blush resistant hardener. We use professional dental impression molds,a two part process, base and catalyst to make the flexible mold (President, polyvinylsiloxane). Then Araldite 506 epoxy with HY 356 hardener is poured and cured at room temp overnight. Sputter coating glass slides with gold palladium does not seem to cause the artifact, but another question, is gold palladium more susceptible to oxide formation than pure gold, and do oxides look like the above described artifact. Also, etching the sample does not help. Any suggesstions will be greatly appreciated. Thanks.
We store Os in a glass bottle with Parafilm around the top, inside a larger plastic jar with a corn oil-soaked paper towel taped to the inside of the lid, and then the outer container is Parafilmed around the top. No black frig. (Change the oil-soaked towel periodically.)
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
I use Denton spatter coater with Au/Pd target for magnifications up to 100,000 without any problems. I can observe collagen striations and other fine details and do not see coating structure. Parameters for coating: current 15 ma, time 5-10 sec. Some charging can occur for samples with "multilayer" surface, but it is tough case for almost any coating anyway. I have also magnetron Cr coater, but do not use it often because it's much more time consuming.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 3127 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu] } Sent: Wednesday, January 05, 2000 10:15 AM } To: 'microscopy-at-sparc5.microscopy.com' } Subject: SEM: Sputter coating } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Hi, } } Due to some recent high-resolution requirements in our lab, I } find myself } having to go back to Sputter Coating 101 (after years of just putting } specimens in the coater and turning it on without a second } thought!). We } find ourselves in need of very thin coatings with as little } structure as } possible, in order to image samples down in the nanometer range. } } We have a chromium coater, but often need to revisit samples } days or weeks } after the initial coating. The oxidation problem rears its } ugly head. We } intend to purchase a platinum target, but don't yet have one, so we're } experimenting with our venerable Au/Pd coater. } } My questions are: } } 1) I seem to remember a string on this listserver suggesting } that lower } deposition currents yield finer coating structure. Is this } right? Does a } low deposition current for a longer time yield a finer } coating than a higher } current for a shorter time? (I'm running some tests to check } this, but } would be very interested in others' experiences, too.) } } 2) Deposition current can be controlled by the initial current setting } (i.e., the knob on the machine) and by the argon flow through } the chamber. } Is there any difference in the coating when adjusting the } deposition current } by either of these two ways? } } 3) Charts I have seen indicate that deposition current is directly } proportional to coating rate. Is the same true for coating } time? I.e., is a } one minute coating twice as thick as a 30 sec. coating? It } would seem so } intuitively, but you know what they say about the word "assume". } } My apologies if these are very basic questions, but, like I } said, back to SC } 101! } } I'll be happy to summarize the responses for anyone who is interested. } } Thanks! } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility } W122 Veterinary Medicine Bldg. } University of Missouri } Columbia, MO 65211 } (573)882-8304 } tindallr-at-missouri.edu } http://www.biotech.missouri.edu/emc/ } }
Posted at author's request to remain anonymous....
} To: zaluzec-at-Sparc5.Microscopy.Com } Date: Mon, 10 Jan 2000 01:20:59 +0530 } Subject: Nestor, would you post this for me? } } Osmium Vapor- A Safety Question } } The last time I mentioned a safety concern about the rumor of an possible } "explosive" warning about some Ruthenium compound , I got "Battered" by } certain vendors(for quite a while too I might add..certainly didn't help } my previously supportive, pro-vendor position-since I used to be one!) . } I was warned and pestered frequently and asked to retract any suggestion } of such a possibility, but I refused. So here we go again.... } } On approximately January 6, 2000 I noticed a request under the } title: } } : Donna Wagahoff {DWAGAHOFF-at-siumed.edu} } } "....Does anyone have experience with osmium stored in } plastic?..." } } and the only responses on the list server were: } } " How about putting the glass containers inside plastic containers? } There are also padded and styrofoam containers which you could use for } the transportation step." } } and } } ......" I store this glass bottle inside an aluminum-foiled plastic } container in my fume hood at room } temp. The clear plastic of this outer container gets translucent black } within weeks no matter how carefully I seal the glass bottle"....... } } ( I'm sure these were very fine suggestions..but..) } } This concerns me for several reasons: } } 1. I believe there is a considerably more dangerous situation here than } many of us may realize, or..will discuss. We must check, and we may find } that Osmium and Ruthenium compounds(and others?) may penetrate all } plastics and we may only see this at a macroscopic level long after the } heavy metal compounds or there derivatives have penetrated many layers } and possible contaminated at some lower and less visible level( but } undetermined danger level that may be a health risk) a much larger area. } Many of us may have seen the effect in EM refrigerators as the interior } blackens over a long period of storage of Osmium. } } 2. There doesn't seem to be an acceptable safe level of these compounds } or known affects, but probably fixation or contamination and loss of } function at some level occurs(microscopic?). } } 3.Also venting these vapors through hoods should be scrubbed or } absorbed(?), reduced,etc.( "I'm no chemist") and the level of the output } probably should be carefully and technically monitored. } } 4. The safety level must be obvious to those who receive bottles of } solutions in sealed glass(or Pirex?) and the factories that manufactor } them.(What glass type materials are safe?) } } Does anyone know of the level of danger(or safety) or know of some good } research sites or periodicals that have dealt with these issues? Any } personal experience, or are we all afraid of retribution and retaliation? } The MSDS's seem a little less than thorough to me. I'm sure I'm again not } alone here in this concern and only wish to continue this difficult and } frequently dangerous craft(EM) with more care for myself , my associates } and my community. } } Anonymous (chicken....) } (...and Vendors and manufacturers are our backbone and our support! } Thank you all!) } } } }
Does anyone have an idea of a good negative film scanner to use for TEM negatives? I am trying to eliminate the darkroom printing process. These are the stipulations: 1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489 film) 2. Must be compatible to a PC. 3. Need to know what software is needed. 4. Must be priced under $3,000.00 5. Must provided the best quality resolution for diagnostic results.
It is our experience, at South Bay Technology, that Cr films deposited by ion beam sputtering remain conductive for a short time. The amount of time depends on the film thickness and sample surface topography. Because Cr deposition requires a water vapor free environment, usually not possible in a sputter coater, you may have more success with Ir target. Under 200Kx, ion beam deposited Ir (better than Pt since Ir films minimize beam damage) does not display any artifacts (grains cannot be seen) or specimen damage. We recommend using Cr (or Ti, W or Ta) only when looking at mag } 200Kx.
An Ir target in your sputter coater may work well and an Ir target in your "Cr coater" may solve the oxidation problem more effectively.
Some advantages of Ion Beam Sputtering: - Controlled thickness on angstrom level since the average deposition rates are 10A/min - Precise thickness measurements reported by quartz thickness monitor as result of low energy sputterant energy striking crystal - No damage or artifacts as a result of 30ev sputterant energy - Any material can be deposited although Cr is suggested for } 200Kx mags, Ir for {200Kx - C like metal films are amorphous. C ddoes not display grains or create damage - X-ray production from 10A films is lost in the noise - Image improvement results from increased signal to noise as well as conductivity
Disclosure: South Bay Technology is the manufacturer of the IBS/E Ion Beam Sputtering and Etching System and therefore has a vested interested interest in promoting it use.
Regards, Mike Mizell
************************************************************************* Michael K. Mizell Tele: 949-492-2600 VP Sales & Marketing Fax: 949-492-1499 South Bay Technology 1120 Via Callejon mizell-at-southbaytech.com San Clemente, Ca 92673 USA ************************************************************************** South Bay Technology is an American manufacturer of precision sample preparation equipment and supplies for metallography crystallography and electron microscopy.
} } } } } } } Please visit us at http://www.southbaytech.com { { { { { { { {
I would like to know what is a nomarski disc. What it is used for? Is there any difference between this and the routine phase contrast condenser we use in light microscopy. Regards, M. Angou SUMKA SONS INSTRUMENTATION1137-B, THADAGAM ROAD,R S PURAM, COIMBATOREINDIA - 641002 FAX: 91-422-473227TEL:91-422-474378 URL:www.sumka.com
first, I would like to wish you all the best for the new year. Now my question: Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?
Is it that delicate as LR-White (Meanwhile I donŦt use LR-W older than half a year). My bottle which was not opened many times, could be roundabout three years old.
Thanks a lot, Michael
Michael Reiner Department of Anatomy I University of Cologne Germany michael.reiner-at-smail.uni-koeln.de
We scan our 4489 negs with a UMAX PowerLook III regularly on a Mac G3. The UMAX is compatable with PCs too, cost about 1100 dollars last summer with the transparancy adaptor and has 1200x2400 dpi actual resolution. We had to modify the 4x5" transparancy holder (easy) to fit the smaller EM negs. UMAX tech help could improve but the scanner works well. Stand alone or PhotoShop plug-in software. Connect via SCSI.
Other units to check out are the Agfa DuoScan T2500 and Microtek ScanMaker 5.
The major electronic trade show meetings are soon so all of the new 'improved' equipment will be ordered and available for summer/fall 2000. The remaining stock of last year's discontinued models will be discount priced by Spring. Good Luck
} Date: Mon, 10 Jan 2000 18:44:54 -0600 } To: Microscopy-at-Sparc5.Microscopy.Com } From: "ELMA Cortinas" {ECORTI-at-childmed.dallas.tx.us} } Subject: Negative film scanner }
} } Does anyone have an idea of a good negative film scanner to use for } TEM negatives? I am trying to eliminate the darkroom printing } process. These are the stipulations: } 1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489 } film) } 2. Must be compatible to a PC. } 3. Need to know what software is needed. } 4. Must be priced under $3,000.00 } 5. Must provided the best quality resolution for diagnostic } results. } } Thanks in advance! }
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility U-2131 Storrs, CT 06269-2131 bsgphy3-at-uconnvm.uconn.edu 860 486-2914 voice 860 486-1936 fax
We are using an Epson Expression 800 and have been very happy with it. We routinely scan in at 600 dpi, which has been more than enough for publication quality, in our experience. The unit is capable of higher resolutions than that. I don't remember what we paid, but it was considerably less than $3000. It came with a transparency adapter and all necessary software, including SilverFast, text recognition software, calibration software, etc. Ours works through Adobe Photoshop's Import functions, but may be usable in other programs, too.
Best wishes, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine Bldg. University of Missouri Columbia, MO 65211 (573)882-8304 tindallr-at-missouri.edu http://www.biotech.missouri.edu/emc/
-----Original Message----- } From: ELMA Cortinas [mailto:ECORTI-at-childmed.dallas.tx.us] Sent: Monday, January 10, 2000 6:45 PM To: Microscopy-at-Sparc5.Microscopy.Com
Does anyone have an idea of a good negative film scanner to use for TEM negatives? I am trying to eliminate the darkroom printing process. These are the stipulations: 1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489 film) 2. Must be compatible to a PC. 3. Need to know what software is needed. 4. Must be priced under $3,000.00 5. Must provided the best quality resolution for diagnostic results.
{fontfamily} {param} Times_New_Roman {/param} {smaller} Postdoctoral position available immediately to study the 3-D structure of insect flight muscle. The research project, recently renewed for a 4 year period, involves several experimental and theoretical approaches to studying crossbridge structure in different states. Approaches include electron microscope tomography, alignment and classification of 3-D crossbridge structures (see recent publication (Winkler & Taylor, Ultramicroscopy 77:141-152 (1999) for details) and fitting of atomic coordinates of actin and myosin S1 into the envelope of the 3-D images. Emphasis is on the study of quick-frozen, contracting muscle, freeze-substituted for thin section electron microscopy and 3-D image reconstruction. We use stretch activated muscle as well as an isometrically contracting state dubbed stretch activation. Specimens are mechanically monitored right up the point of freezing to facilitate the interpretation of the structures in terms of muscle force and stiffness. Parallel X-ray diffraction experiments make for thoroughly characterized specimens. Please see recent publication (Taylor et al., Cell 99:421-431 (1999)) for current status of this project. This project is a collaboration with Michael and Mary Reedy (Duke Univ. Med. Center), Yale Goldman and Clara Franzini-Armstrong (U. Penn. Med. School) and Richard Tregear (MRC, Cambridge, UK). Successful applicants can work on any of several aspects of the problem of identifying structural features and relating them to the generation of tension in muscle. The project has evolved to the point that most of the effort needs to be put on classification and averaging, model building, model refinement and interpretation of X-ray data. An individual with experience in either image reconstruction or protein structure and function who is interested in gaining further experience in the interpretation and correlation of diverse experimental data on a topical biophysical problem would be ideal for this position. The experimental emphasis of correlating high resolution structures with lower resolution EM data is expected to become a growth area for structural biology. Our laboratory is equipped with Silicon Graphics workstations, one of which is dedicated to this position, a cluster of 3 DEC Alpha compute servers, a Perkin-Elmer PDS 1010M microdensitometer and a Philips CM300-FEG electron microscope. Salary is commensurate with relevant experience. Successful candidates will join a strong Program in Structural Biology with 4 groups using primarily X-ray diffraction, 3 using NMR, 2 using EPR and one using EM. The Program enjoys close ties with the National High Field Magnetic Laboratory and the Supercomputer Computation Research Institute on campus. Additional information about the Structural Biology Program can be found at http://www.sb.fsu.edu/. Interested applicants should send their CV and names, addresses and phone numbers of 3 references to Dr. Kenneth A. Taylor, Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA. E-mail address is taylor-at-bio.fsu.edu. Phone number 1-850-644-3357, FAX 1-850-561-1406.
The Institute of Molecular Biophysics and Florida State University are located in Tallahassee, the capitol of Florida. The city has a population of ~200,000. The city is surrounded by rolling hills and pine forests and is 35 miles from pristine beaches on the coast of Gulf of Mexico. Tallahassee residents enjoy many cultural and sporting events.
Posted at author's request to remain anonymous....
Dear Anon,
} Osmium Vapor- A Safety Question } } The last time I mentioned a safety concern about the rumor of an possible } "explosive" warning about some Ruthenium compound , I got "Battered"... } The EM Safety Handbook also warns of the risk of explosion from RuO4.
} On approximately January 6, 2000 I noticed a request under the } title: } } : Donna Wagahoff {DWAGAHOFF-at-siumed.edu} } } "....Does anyone have experience with osmium stored in } plastic?..." } } and the only responses on the list server were: } } " How about putting the glass containers inside plastic containers? } There are also padded and styrofoam containers which you could use for } the transportation step." } } and } } ......" I store this glass bottle inside an aluminum-foiled plastic } container in my fume hood at room } temp. The clear plastic of this outer container gets translucent black } within weeks no matter how carefully I seal the glass bottle"....... } } ( I'm sure these were very fine suggestions..but..) } } This concerns me for several reasons: } } 1. I believe there is a considerably more dangerous situation here than } many of us may realize, or..will discuss. We must check, and we may find } that Osmium and Ruthenium compounds(and others?) may penetrate all } plastics... } This is very likely.
} 2. There doesn't seem to be an acceptable safe level of these compounds } or known affects, but probably fixation or contamination and loss of } function at some level occurs(microscopic?). } The EM Safety Handbook gives a TLV--threshold limit value, defined as a concentration "to which *it is believed* (emphasis is mine) *nearly* all workers may be repeatedly exposed day after day without adverse effects."--of 2parts in 10^10. Note the very small value and the cautionary wording.
} 3.Also venting these vapors through hoods should be scrubbed or } absorbed(?), reduced,etc.( "I'm no chemist") and the level of the output } probably should be carefully and technically monitored. } Since OsO4 is a powerful oxidant, and since it reacts with unsaturated lipids, I had heard the recommendation that corn oil be used to clean up spills; however, the EM Safety Handbook recommends ascorbate powder "as it reacts quickly", and, come to think of it, is more suited for treating an aqueous solution than is an immiscible oil.
} 4. The safety level must be obvious to those who receive bottles of } solutions in sealed glass(or Pirex?) and the factories that manufactor } them.(What glass type materials are safe?) } I doubt that any particular glass is less safe (but would not be surprised to be corrected). Any glass which doesn't oxidize shouldn't react with OsO4.
} Does anyone know of the level of danger(or safety) or know of some good } research sites or periodicals that have dealt with these issues? Any } personal experience, or are we all afraid of retribution and retaliation?
The extensively-quoted EM Safety Handbook is a good source.
} The MSDS's seem a little less than thorough to me.
Although, the existance of an MSDS for di-hydrogen oxide (flamed here some time ago) would seem to argue otherwise. There is further info available from company web sites and phone lines (listed in some instances on the MSDS), and your safety office should have other relevant info.
} I'm sure I'm again not } alone here in this concern and only wish to continue this difficult and } frequently dangerous craft(EM) with more care for myself , my associates } and my community. } } Anonymous (chicken....) } (...and Vendors and manufacturers are our backbone and our support! } Thank you all!) } I'd say the first order of safety is to be concerned, next is to get all the info available. Yours in chickenhood, Bill Tivol
This technology needs more thorough discussion, I believe. There are several rather serious safety issues that, I believe, we might find some better solutions to here, if we open this area for discussion . For example vapor level and detection, penetration, permeability, scrubbing, reactive absorption, isolation, etc.. Some plastics stain easily and some do not, but all are apparently permeable. On the productive side the commercially astute might find several new product ideas valuable to this community. 'JD'
previously:
Plastic is oxidized and so part of the osmium is exhausted in the vials. Even when frozen osmium diffuses through plastic containers. So you may save a rare breakage, but you are certain to have osmium in your refrigerator. It's just a bad idea to pack osmium in plastic. You could use a secondary container, be it plastic or glass, to increase overall safety. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Thursday, January 06, 2000 3:08 AM, Donna Wagahoff [SMTP:DWAGAHOFF-at-siumed.edu] wrote: } } We have always kept our osmium solutions in glass containers. However, we } are in the process of evaluating our safety procedures and discussed the } idea of increasing the safety of the transport of osmium from the } refrigerator to the fume hood by putting the osmium solution in plastic } containers. (If they are dropped, they would not break and cause the danger } of a spill outside the hood.) } Does anyone have experience with osmium stored in plastic? Any comments } about this particular subject or any of your safety with osmium procedures } are welcomed. } Thanks. } } Donna Wagahoff } SIU School of Medicine } PO Box 19627 } Springfield, IL 62794-9627 } 217-782-0898 } fax217-524-3227
Recently an E3 Electroscan has become available and I'm becoming aware of a much different SEM technology than I'm used to seeing over the last 30 years. It obviously operates only at vacuum levels in the torr range, right? I am still a little confused how resolution can be maintained in these vacuum levels. And dispersive X-ray spectroscopy, does this broaden the peaks and what happens to spectral resolution? It also appears that this particular design cannot pump down below 10-4(?). There are some complex gas background issues that are different. Are there ways of using these design parameters to the benefit of the materials imaging analysis in samples that are not hydrated or partially volatile? 'JD'/Texas
Continuing in the line of ICPIC '95 held at IIT, Kharagpur and ICVGIP '98 held at IIT, Delhi, ICVGIP 2000 will be organized by the Centre for Artificial Intelligence & Robotics at Bangalore during December 20-22, 2000. The conference is intended to bring together the Vision, Graphics, and Image Processing communities together with a special emphasis on India. A high quality technical track will be augmented by presentations from various R&D institutions in the country and the industry.
Important Dates: ----------------
Submissions due: May 15, 2000 Notifiation of acceptance: Sep 01, 2000 Final papers due: Oct 15, 2000 Conference dates: Dec 20-22, 2000
Topics: -------
We strive to host a high quality conference in India. An additional goal his to bring the community of Indian practitioners of these areas together at a single forum. We encourage papers related to system development, innovative applications etc., in addition to research papers. We especially encourage papers by student. The topics of interest include, but are not limited to, the following:
Electronic submissions are highly encouraged. Acceptable formats are: Acrobat PDF, standard PostScript, self-contained LaTeX with psfig, and Word 7.0. Check the official web page for details on electronic submission. Manuscripts should not exceed 20 double-spaced pages including figures and tables. The submission should include a cover page with the title, the authors' names, abstract and keywords. Those submitting hard-copy manuscripts should send four copies to the following address:
ICVGIP 2000 Secretariat Centre for Artificial Intelligence & Robotics (CAIR) Raj Bhavan Circle, High Grounds Bangalore, 560 001. INDIA
-------------------------------------------------------------------- Patrons: -------- Prof. M. Vidyasagar, CAIR Prof. R. Narasimha, NIAS
General Chair: -------------- Dr. P. J. Narayanan, CAIR pjn-at-cair.res.in
Program Co-Chairs: ------------------ Prof. Ramakant Nevatia, USC nevatia-at-usc.edu Prof. Jayanta Mukherjee, IIT, KGP jay-at-cse.iitkgp.ernet.in
Organizing Chair: ----------------- Prof. Swamy Manohar, IISc manohar-at-csa.iisc.ernet.in
Plenary Chair: -------------- Dr. P. Anandan, Microsoft anandan-at-microsoft.com
Publications Chair: ------------------- Dr. C. V. Jawahar, CAIR jawahar-at-cair.res.in
Treasurer: ---------- Dr. Subrata Rakshit, CAIR subrata-at-cair.res.in
-------------------------------------------------------------------- Organized by Centre for Artificial Intelligence and Robotics (CAIR) --------------------------------------------------------------------
Hi all- I am looking for someone to provide in house service for a Denton Sputter Coater in the Rhode Island area. Please feel free to contact me offline.
---------- } From: c j day {wa5ekh-at-juno.com} } To: Microscopy-at-sparc5.microscopy.com } Subject: ESEM } Date: January 11, 2000 10:24 PM } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Recently an E3 Electroscan has become available and I'm becoming aware } of a much different SEM technology than I'm used to seeing over the last } 30 years. It obviously operates only at vacuum levels in the torr range, } right? I am still a little confused how resolution can be maintained in } these vacuum levels. And dispersive X-ray spectroscopy, does this } broaden the peaks and what happens to spectral resolution? It also } appears that this particular design cannot pump down below 10-4(?). } There are some complex gas background issues that are different. Are } there ways of using these design parameters to the benefit of the } materials imaging analysis in samples that are not hydrated or partially } volatile? } 'JD'/Texas } } This is exactly the model we've been using here for the past 7 or so years. In fact, the instrument can be operated in "normal" high vacuum mode, too (if your samples don't mind it). The resolution is not bad, even in "wet" mode - say, 2 - 10 torr. There is a certain amount of beam diffusion, of course, in a wet atmosphere, but we've been doing EDS for the past 5 or 6 years with pretty good, reproducible, results. (We have a NORAN ultra-thin window detector and Voyager 3 software.) Since we have a LaB6 gun in ours, there is also an ion pump, and the gun vacuum is maintained at a little better than 10 -6 torr. As you know, the instrument can be used with, instead of water vapour, inert gases as an atmosphere in the chamber. As it happens, we don't do that with our instrument, so I couldn't really comment. I suspect that there probably aren't any major advantages in using an instrument like this for materials studies, except that it has a very large specimen chamber that can accomodate several types of stage. And, of course, the fact that samples generally require no coating before examination. Much of our usage is earth sciences, and it's nice not to have to coat type fossils with carbon or metals. There are two major disadvantages with the E3's. One is that the field of view is very small - you can't really image anything larger than about 1 mm in length or diameter, because of the configuration of the ESD detector/final aperture assembly. This can be a major pain. Another is that, with the standard stage, samples can not be thicker than about 25 mm or so, especially if you want to do EDS. FWIW, our instrument has been dead reliable since it's installation. Other than biannual column cleanings, the odd hose leak, and an occasional glitch with some miscellaneous part, the machine is hardly ever down. (Kind of like my Harley - there may even be a few shared parts :-). If you do wind up acquiring the E3, the Philips service rep for the American southeast is (or at least was a couple years ago) Steve Booth. (You probably know that Philips bought out ElectroScan a few years ago, so they handle the service contracts now). Steve was here twice to do service calls on ours, because both times, the regular US Northeast guy was unavailable. Steve runs a horse ranch somewhere in Texas, I believe, but knows his E3's pretty well, too. This might be a whole lot more than you wanted to know, but I'll admit to being a bit of a fan of our instrument - like my bike, it's ruggedly built, but is no more complex than it has to be. Just last week, myself and a local SEM service tech who'd never seen an ESEM before completed a biannual column cleaning, and it all went very well - takes a day or two to get the gun vacuum down to operating levels again, though.
No connection with Philips Electron Optics, etc......
F.C. Thomas MicroAnalysis Facility Geological Survey of Canada Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada B3L 4C8
Dear List, can you help me once again. Can anyone recommend a sub $1500 video camera for video microscopy. It will be generally used for relatively high intensity fluorescence microscopy (imaging GFP bacteria) and basic microscopy. At present we have a Sony XC-999P (752x582 pixels) and are looking to upgrade - preferably in resolution and sensitivity - but resolution is the most important factor. If people wish they can respond off=list and I will produce a prcis of the information I receive. Thanks. -------------------------------------------------------------------------------- -----------------------Dr. Giles SandersZeneca / SmithKline Beecham Centre for Analytical SciencesChemistry DepartmentImperial College of Science, Technology and MedicineLondonSW7 2AY (44) - 0171-594-5749 Never express yourself more clearly than you think. -- Niels Bohr (1885-1962) Danish physicist -------------------------------------------------------------------------------- --------------------
Hello All! I guess National Geographic Explorer Magazine will be showing some of David Scharf's work in the next "Explorer" show on TBS (Turner Broadcasting System-yes it's not the Braves or Clint Eastwood!) on January 16th. They will show some of his work imaging parasites with the modified SEM. Should be cool! I love it when they show electron microscopy on TV. Just thought you might want to know!
Tracey Pepper Supervisor Bessey Microscopy Facility Iowa State University ph: 515-294-3872 fax: 515.294.1337
A few of the simpler advantages of an ESEM (we have a model E3): No need to coat a sample saves a few minutes (at least) allows for back-and-forth work with a light microscope No need to pre-pump to remove volatiles - the differential pumping system handles this (although your rough pump oil becomes your trap) Gas evolution (degradation on heating, for example)is not a problem (see above) Aging/dynamic studies don't get compromised due to sample coating. near-atmospheric pressure minimizes sublimation without cryogenics You can study influence of water (swelling, for example)or other sample/gas interactions ESD detector is light-insensitive, so you can watch the sample as you position it. Also as you poke/prod it with the micromanipulator option.
As for EDX, yes, there is a significant beam spread from the imaging gas. I typically line up the region-of-interest, then dial the chamber pressure to zero before collecting spectra. The spread is significantly reduced.
Without trying to touch off arguments, my practical experience is that above about 20,000X I don't collect images worth writing home about. They are useful, but not beautiful.
It's an instrument that fills an interesting niche, even for a materials scientist. (The real forte' is biological/wet stuff. That's where the fun really begins!)
Bill Heeschen The Dow Chemical Company
-----Original Message----- } From: c j day [mailto:wa5ekh-at-juno.com] Sent: Tuesday, January 11, 2000 9:24 PM To: Microscopy-at-Sparc5.Microscopy.Com
Recently an E3 Electroscan has become available and I'm becoming aware of a much different SEM technology than I'm used to seeing over the last 30 years. It obviously operates only at vacuum levels in the torr range, right? I am still a little confused how resolution can be maintained in these vacuum levels. And dispersive X-ray spectroscopy, does this broaden the peaks and what happens to spectral resolution? It also appears that this particular design cannot pump down below 10-4(?). There are some complex gas background issues that are different. Are there ways of using these design parameters to the benefit of the materials imaging analysis in samples that are not hydrated or partially volatile? 'JD'/Texas
Does anyone have a method for flattening 0.5 um thick epoxy (Spurr) sections for LM? We are using water pickup and mild heat drying onto glass slides and that doesn't seem to be doing the trick.
TIA
Bob Dr. Robert R. Wise Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (920) 424-3404 wise-at-uwosh.edu http://www.uwosh.edu/departments/biology/wise/wise.html
I want to look at what I hypothesize is a densely packed array of membranes in TEM. In routine osmium fixed, LR White and Epon embedded specimens, the image is not overwhelming. I plan to try ruthenium tetroxide ala the skin people looking at lamellar bodies. I am wondering whether I am missing another obvious approach. Any ideas gratefully accepted. Tom
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
I am currently working on preparing TEM foils of single crystalline gold. The electropolishing was completely fruitless, until I tried Bernie Kestel's solution "BK-2". This has worked wonders, giving a very smooth and shiny surface. FYI, I use a Struers Tenupol twin-jet electropolisher, so my conditions are slightly different than with the South Bay polisher. One problem remains: the electron-transparent edges of the foil are very prone to bending, and thus I have regions that are full of bend contours. This isn't terribly surprising, since the gold is from a grown single crystal and has been subjected only to about 1% plastic strain. Any ideas on reducing the amount of bending, so that I can see the dislocations more easily? Are there any specific profiles for foil perforations that will help keep the edges rigid (other than a smooth, small hole)? Any ideas, either for improving specimen prep, or for "fixing" foils I already have, would be greatly appreciated.
Regards,
John -- ____________________________________ John Balk 200 Latrobe Hall Johns Hopkins University 3400 N. Charles St. Baltimore, MD 21218 ph: (410) 516-8284 fax: (410) 516-4316 e-mail: balk-at-kjhsgi.me.jhu.edu
We are in the market for a CCD camera for a JEOL 1200CX TEM. I would appreciate any information regarding to this type of products available on the market. Thank you.
Those of you who know me can understand that I agree completely with JDs comment below. The whole issue of safety in handling laboratory chemicals is one that, I believe, has still not been completely addressed and accepted by everyone. We all know the short term effect that exposure to toxic levels of OsO4 can have on a person, eyes is one that comes to mind. Formaldehyde and glutaraldehyde are others that I and a pathologist, who I just met yesterday, are now well aware of its possible long term effects. But what about long term, synergistic effects of some chemicals that are by themselves relatively innocuous? Combinations of two, three, four? The only answer is to develop and follow safely procedures for handling each chemical as if it were very toxic. There is no reason not to, you already do it for some chemical, and there are many reasons to do so.
For those who don't know me and need a little motivation, think "bilateral, single sequential lung transplant" .
Damian
At 07:55 PM 1/11/00 -0600, c j day wrote:
} This technology needs more thorough discussion, I believe. There are } several rather serious safety issues that, I believe, we might find some } better solutions to here, if we open this area for discussion . For } example vapor level and detection, penetration, permeability, scrubbing, } reactive absorption, isolation, etc.. Some plastics stain easily and some } do not, but all are apparently permeable. } On the productive side the commercially astute might find several } new product ideas valuable to this community. } 'JD'
What is the longterm effect of exposure to osmium on the brittleness and permeability of common plastics? We leave exposed polymer block faces in osmium vapour overnight before sectioning.
Hello all! We are looking for an ESEM in the greater Washington DC area for a limited amount of work (maybe a dozen samples over a two-month period). This could be a contractual situation, although we are rather hoping for the owner's generosity.... Please reply off-list to: rjpalmer-at-dir.nidcr.nih.gov Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 310 30 Convent Drive Bethesda MD 20892 ph 301-496-2088 fax 301-402-0396
I have a Reichert compound microscope that needs servicing. The number I have for the company who used to service my microscope is no longer valid. Is there anyone in the RTP, NC area who could recommend a service vendor? Please respond to gail.harrison-at-reichhold.com
Does anyone have a solution for this problem? I managed to get a spot ( {3mm around) of Toluidine Blue on the cuff of my new shirt (the only part extending beyond my lab coat). Is there any way to get rid of all (or most) of the stain without it spreading? The shirt is 100% cotton. (I don't care if it gets on my lab coat, but this is the first time in over fourteen years that I've gotten it on my clothes.)
If I don't get any response, my inclination is to try a sodium tetraborate paste applied with a cotton swab.
Jaclynn Lett, Research Assistant jmlett-at-cid.wustl.edu
Fay and Carl Simons Center for the Biology of Hearing and Deafness Central Institute for the Deaf 818 S. Euclid Ave. St. Louis, MO 63110
I am having great difficulty extracting large carbides from a steel specimen using the standard single stage carbon extraction technique. The film is either not releasing or it is breaking up into unusable pieces. My standard practice is as follows:
1) Etch polished surface in 2% Nital for 15 to 45 seconds 2) Release sections in 10% Nital 3) Float sections in Dist. water and retrieve
I have used an electrolytic process to aid in releasing the sections but often this process tends to create somewhat dirty replicas.
I also am going to try a two-stage replication technique, but would prefer to have sucessful single stage replicas.
Does anyone have any suggestions that would be of assistance in my single stage replication technique? Thank you for your consideration.
Kevin Downey Research Analyst Bethlehem Steel Corp. e-mail: rkedo-at-bsco.com
} Does anyone have a solution for this problem? I managed to get a spot ( {3mm } around) of Toluidine Blue on the cuff of my new shirt (the only part } extending beyond my lab coat). Is there any way to get rid of all (or most) } of the stain without it spreading? The shirt is 100% cotton. (I don't care } if it gets on my lab coat, but this is the first time in over fourteen years } that I've gotten it on my clothes.)
We have some stuff called Erada-Stain. Its made for histological stain removal from hands, glassware, etc. We've had our tube of it for forever(15 years +) but it seems like it would be one of those things you should still be able to find. Its always worked for me when I had a clothes splash. Good Luck
Paula Moore Wake Forest Univ. Baptist Medical Center EM Lab pmoore-at-wfubmc.edu
Embeded in TIFF images is data describing the "print" size, X inches by Y inches at N dpi. Of course, this is directly related to the pixel matrix size.
My problem is that my digital imaging equipment (Hitachi S-3500 and IXRF EDS) saves the image data at 72 (or less) dpi. The pixel array size is correct, but at {= 72 dpi, many programs (like PhotoShop) want to print a huge image.
I can overcome this by (in P.S. for example) resetting the image to 300 dpi and adjusting the print size so that the pixel array is not altered. At the very least, this is cumbersome and time consuming when a large number of images must be printed.
I would like to find a way to change the file saving default value for the dpi to avoid image resizing for most print applications.
I've received a lot of inquiries about the education potential of the $100 (or less, in some stores) toy digital microscope introduced by Mattel just before Christmas; it's a plastic-bodied scope with simple image processing software which requires a wired connection to a Windows 98 computer (I'm a Mac user and I don't often feel envious, but...). It's been hard to get good information, but Jim Harper has just posted an excellent article on the web. He describes its capabilities well - far better than any of the other reviews that I've read. And he gives detailed instructions on how to mount it on ANY light microscope! Don't miss the hotlinks at the end of his piece.
Educationally, it's no substitute for the one student - one microscope approach of Project MICRO and "Microscopic Explorations", its manual. But it has exciting potential for classroom demonstrations and science fair projects. And listserver readers who are looking for low cost digital recording of LM may find that it's adequate for a lot of applications. Please let us know if it works for you.
The site is http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
I am soliciting contributors (or names of potential contributors) for a symposium for the natiional Microscopy & Microanalysis Annual Meeting to be held on August 13-17, 2000 in Philadelphia, Pa.
Talks may range in length from 25 to 45 minutes. Deadline for receipt of 2 page absracts is Feb 15, 2000.
A description of the symposium follows.
SYMPOSIUM: MICROORGANISMS: THE GOOD, THE BAD, THE UNUSUAL
This symposium will deal with microorganisms (viruses, bacteria, parasites, prions) found in the environment as well as in higher life forms (animals and plants). Newly discovered pathogens or organisms with unique capabilities (detoxification, invasiveness, resistance to antibiotics) are of interest in this symposium. Of particular interest are those orgamisms that represent extremes, as for example: the ability to grow in extreme environments, having extreme virulence or invasiveness, or being difficult to visualize using conventional prepartory procedures. Hopefully, the participants shall describe some of the features of extreme organisms that give rise to these capabilities. Finally, many of these organisms are often difficult to visualize using standard preparatory procedures. Papers describing procedures to prepare the specimens for visualization would be germane to this symposium.
==============================================
#################################################################### John J. Bozzola, Ph.D., Director Micro-Imaging and Analysis Center 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
} Hello All, } } Embeded in TIFF images is data describing the "print" size, X inches by Y } inches at N dpi. Of course, this is directly related to the pixel matrix } size. } } My problem is that my digital imaging equipment (Hitachi S-3500 and IXRF } EDS) } saves the image data at 72 (or less) dpi. The pixel array size is correct, } but } at {= 72 dpi, many programs (like PhotoShop) want to print a huge image. } } I can overcome this by (in P.S. for example) resetting the image to 300 dpi } and } adjusting the print size so that the pixel array is not altered. At the } very } least, this is cumbersome and time consuming when a large number of images } must } be printed. } } I would like to find a way to change the file saving default value for the } dpi } to avoid image resizing for most print applications. }
I also have a Hitachi S-3500N. Use the Action Palette in PhotoShop to change the print size and adjust the pixel array with a single key stroke.
Rick A. Harris, Director Microscopy and Imaging Facility Section of Molecular and Cellular Biology 1241 Life Sciences Addition University of California Davis, CA 530 752 2914 530 754 7536 fax http://katie.ucdavis.edu raharris-at-ucdavis.edu
I suggest getting a hold of ThumbsPlus. I print everything from it. It can stretch an image to full scale and can print annotation text with the image. It is a graphics database program that works very well. In addition, it can convert from almost any format to any other format.
What it can do for you is to convert your image from 72 dpi -big to 300 -small and keep it in the same format, e.g. Tiff. You can do a batch convert easily.
Find out more at www.cerious.com
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
} -----Original Message----- } From: White, Woody N [mailto:Woody.N.White-at-mcdermott.com] } Sent: Thursday, January 13, 2000 1:56 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: TIFF image dpi format ? } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } -------------------------------------------------------------- } ---------. } } } Hello All, } } Embeded in TIFF images is data describing the "print" size, } X inches by Y } inches at N dpi. Of course, this is directly related to the } pixel matrix } size. } } My problem is that my digital imaging equipment (Hitachi } S-3500 and IXRF } EDS) } saves the image data at 72 (or less) dpi. The pixel array } size is correct, } but } at {= 72 dpi, many programs (like PhotoShop) want to print a } huge image. } } I can overcome this by (in P.S. for example) resetting the } image to 300 dpi } and } adjusting the print size so that the pixel array is not } altered. At the } very } least, this is cumbersome and time consuming when a large } number of images } must } be printed. } } I would like to find a way to change the file saving default } value for the } dpi } to avoid image resizing for most print applications. } } Any suggestions??? } } Thanks, } Woody White } McDermott Technology }
I have a problem doing x-ray mapping on my Philips XL30 (W) with an attached Oxford ISIS 200 EDS system. I'm running the ISIS software on the same pc that controls the XL30, a 133 Pentium with 32Mb memory, running NT4 with service pack 3. I have ISIS v3.32 and XL v5.5 software. When I try to collect say 6 elemental maps using the SPEEDMAP software the system locks after 2 or 3 scans and the computer has to be re-booted. I have tried increasing the memory up to 80Mb but this had no effect. I did not have this problem when running Windows 3.1, although the system would give 'out of memory' errors which is why I upgraded to NT. The problem also occurs when collecting an image using the ISIS AUTOBEAM software and integrating several frames. Single frame acquisitions are ok. If you have a similar system configuration would you please let me know it you experience this problem? I know of stand alone NT systems that are ok, so can only assume it is some conflict with my particular software/hardware combination.
Thanks
Dave
Dave Phelan EM/X-Ray Unit University of Newcastle NSW 2308 AUSTRALIA Ph 02 4921 5667 Fax 02 4921 7019 emudp-at-mail.newcastle.edu.au
We are having antifield systems from Lindgren RF Enclosures , fitted on our two Hitachi FESEMs, an S-4500II and an S-900.
It would be very advisable for the installing engineer to inspect columns of these models before they set out for Sydney.
Could any operator of these models around New York who would allow inspection of their microscopes please mail me?
thanks,
Dr. Mel Dickson, Deputy Director, The Electron Microscope Unit, Adjunct Associate Professor, School of Microbiology & Immunology The University of New South Wales Sydney, Australia. Phone +612 9385 6383 Fax +612 9385 6400
Revisiting an old chestnut - safety and aldehyde fixatives.
In the UK, the Health & Safety Commission have just lowered exposure limits for glutaraldehyde.
Formalin/formaldehyde has a MEL (maximum exposure limit) of 2 ppm or 2.5 mg/cu m - this is measurable and legally enforcible. Glutaraldehyde used to have an OES (occupational exposure standard) of 0.2 ppm or 0.83 mg/cu m. over a 15 minute period. OES is a standard to aim for, but not prosecutable if you weren't achieving it.
Now, glutaraldehyde suddenly has a MEL of 0.05 ppm, both for 15 minute short term reference limit AND THE 8 HOUR TIME WEIGHTED AVERAGE EXPOSURE!! This is a quarter of what it previously was and 40 times lower than that for formaldehyde! Does anyone know why or have evidence or anecdotes of glutaradehyde-related health problems? Is it so nasty??
I appreciate that it is used in bulk as a bacteriocide in hospitals and possibly in horticulture/agriculture.
I am trying to contact HSC specialist committees for a response and will post anything that I receive. I suspect that may be very little.
Regards - Keith (reincarnated after "early retirement")
PS - Hello, Daniele! And those who know her! _______________________________ Dr. Keith Ryan Marine Biological Association of the UK The Laboratory Citadel Hill Plymouth Devon PL1 2PB England
Tel. ++44 (0)1752 633249 Tel. ++44 (0)1752 633279 The 279 number has an answering machine
There may be some confusion about my question. Perhaps I can clarify....
I can resize/fix the image size parameters ok. Either totally manually or with a macro from PhotoShop, etc.
My goal is to NOT have to do that. If the original images are SAVED at the appropriate print dpi this would not be required.... That is my goal. That is... Is there any way to modify the SAVING software (Hitachi PC-SEM/IXRF Iridium) so that the images are, for example, 300 dpi rather than 72 or 26 which is what the software(s) does now.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Does anyone have a solution for this problem? I managed to get a spot ( {3mm } } around) of Toluidine Blue on the cuff of my new shirt (the only part } } extending beyond my lab coat). Is there any way to get rid of all (or most) } } of the stain without it spreading? The shirt is 100% cotton. (I don't care } } if it gets on my lab coat, but this is the first time in over fourteen years } } that I've gotten it on my clothes.) } } We have some stuff called Erada-Stain. Its made for histological stain removal } from hands, glassware, etc. We've had our tube of it for forever(15 years +) } but it seems like it would be one of those things you should still be able to } find. } Its always worked for me when I had a clothes splash. } Good Luck } } Paula Moore } Wake Forest Univ. Baptist Medical Center } EM Lab } pmoore-at-wfubmc.edu } } } Hi,
To destain a slide which has been contrasted with toluidine blue (or any of the other blues), soak the slide in acid alcohol. To one liter of 70% ethanol, add 10ml of conc hydrochloric acid. Soak the cuff in that. If that does not do it, paint your cuff. I have done this frequently. Use laundry marking stick or magic marker or whatever. If I have a lot of staining to do, I simply wear a multicolor blue blouse. No problem.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Does anyone have a solution for this problem? I managed to get a spot ( {3mm } } around) of Toluidine Blue on the cuff of my new shirt (the only part } } extending beyond my lab coat). Is there any way to get rid of all (or most) } } of the stain without it spreading? The shirt is 100% cotton. (I don't care } } if it gets on my lab coat, but this is the first time in over fourteen years } } that I've gotten it on my clothes.) } } We have some stuff called Erada-Stain. Its made for histological stain removal } from hands, glassware, etc. We've had our tube of it for forever(15 years +) } but it seems like it would be one of those things you should still be able to } find. } Its always worked for me when I had a clothes splash. } Good Luck } } Paula Moore } Wake Forest Univ. Baptist Medical Center } EM Lab } pmoore-at-wfubmc.edu } } } Hi,
To destain a slide which has been contrasted with toluidine blue (or any of the other blues), soak the slide in acid alcohol. To one liter of 70% ethanol, add 10ml of conc hydrochloric acid. Soak the cuff in that. If that does not do it, paint your cuff. I have done this frequently. Use laundry marking stick or magic marker or whatever. If I have a lot of staining to do, I simply wear a multicolor blue blouse. No problem.
Greetings friends, A researcher wants to know how to stain for, or find by TEM, Barr bodies that are already prepped and embedded in resin for TEM. Any advise that you can supply will be most appreciated. Thanks, Linda M. Fox Loyola University Stritch School of Medicine Core Imaging Facility 2160 S. First Ave. Maywood, Il 60153 Bld. 102 Room 0617 1-708-216-3395 lfox1-at-wpo.it.luc.edu
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I spent a lot of time many years ago working on 316 stainless. Many of the specimens I made were single stage carbon extraction replicas. The technique I found most effective was to use dilute hydrochloric acid and electrolytic activation. I used this both to etch the surface prior to carbon coating and also to release the carbon replica. I also used the same process on ferritic steels. It was very effective it even released large sheets of M23C6 carbides from grain boundaries. The only 'precipitate' it would not work on was ferrite in austenite.
regards, -- Larry Stoter 17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, UK email: LPS-at-teknesis.demon.co.uk, Home Phone/Fax: +44 (0)1825 767967
Position open at the Australian National University , Canberra www.anu.edu.au/hr/jobs
RESEARCH SCHOOL OF BIOLOGICAL SCIENCES ELECTRON MICROSCOPY UNIT ELECTRON MICROSCOPIST ANU OFFICER GRADE 7 (TECHNICAL) $43,506 - $47,010 per annum (Plus generous superannuation provisions)
Reference No: G000011. The ANU Electron Microscopy Unit, a multidisciplinary research and teaching support facility with four SEMs, three TEMS and ancillary equipment, requires a skilled and experienced person to join its team of 5-6 staff. The successful applicant will have a history of work in electron microscopy in a diversified research-oriented environment, preferably with some administrative experience, and areas of expertise that support and complement those of existing staff. They will have up-to-date expertise in a number of areas of electron microscopy and image analysis. Among these, experience with quantitative energy-dispersive X-ray analysis, research projects in plant or animal cell biology, and cryopreparation techniques is a necessity.
The ANU EMU website is http://online.anu.edu.au/EMU
Contact for selection documentation: Ms Susan Toscan , ph (02) 6249 4752, email: susan.toscan-at-rsbs.anu.edu.au For further information contact: Dr Sally Stowe, email: stowe-at-rsbs.anu.edu.au Closing date: 31st January 2000.
Tenure-Track Faculty Position in Condensed Matter: Electron Microscopist. The candidate should have a strong background in transmission electron microscopy and diffraction, and an interest in the applications of advanced TEM techniques to materials physics. The candidate would be expected to have a broad knowledge of electron diffraction theory, of high resolution microscopy and electron spectroscopy and of materials physics. Possible areas of interest include (but are not limited to), microscopy of magnetic and/or superconducting materials, including holography; defects and interfaces in materials; ferroelectrics; diamond films and film growth; nanoscale materials; amorphous materials; quantitative microscopy; and 3-D tomography. Although not essential, an interest in electron optics would be valuable. The candidate will have the opportunity to establish a joint program with the Electron Microscopy Center in The Materials Science Division at Argonne National Laboratory. Send curriculum vitae and references by March 17, 2000 to: Physics Dept., NIU, DeKalb, IL 60115, Attn: J. C. Shaffer, Chairman. NIU is an AA/EEO Institution.
Scripps Institution of Oceanography Analytical Facility
http://sioaf.ucsd.edu/flyer/
----- Original Message ----- } From: {"rfelten-at-Macdermid.com"-at-sparc5.microscopy.com} To: {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, January 05, 2000 2:16 PM
Dear colleagues,
Would you please give us some information about a beam blanking device and a cryostat which can be set inside the microscope chamber. In fact, we would like to modify our old Phillips microscope (SEM 505) with these two devices. In particularly, could you give us the quotation for these two devices,
Thanking you in advance, Cordially,
email adresse for the answer : abdelillah.elhdiy-at-univ-reims.fr
I've received a lot of inquiries about the education potential of the $100 (or less, in some stores) toy digital microscope introduced by Mattel just before Christmas; it's a plastic-bodied scope with simple image processing software which requires a wired connection to a Windows 98 computer (I'm a Mac user and I don't often feel envious, but...). It's been hard to get good information, but Jim Harper has just posted an excellent article on the web. He describes its capabilities well - far better than any of the other reviews that I've read. And he gives detailed instructions on how to mount it on ANY light microscope! Don't miss the hotlinks at the end of his piece.
Educationally, it's no substitute for the one student - one microscope approach of Project MICRO and "Microscopic Explorations", its manual. But it has exciting potential for classroom demonstrations and science fair projects. And listserver readers who are looking for low cost digital recording of LM may find that it's adequate for a lot of applications. Please let us know if it works for you.
The site is http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
FIXED TERM - Total Remuneration: Level 3 (38 hours): A$35,824 - A$41,251 per year. (Salary Level 3: A$30,272 - A$34,858 per year plus up to 17% employer superannuation plus leave loading.)
The Electron Microscope Unit is a central infrastructural research facility, containing nine principal instruments, which support a wide range of projects. The Unit seeks a self-motivated, enthusiastic person to offer technical support to assist in the smooth running of the Unit. The successful applicant will be required to perform a range of routine laboratory tasks such as film processing, specimen preparation and assist users of the Unit with operation of microscopes.
Essential criteria: familiarity with the operation of both scanning and transmission electron microscopes and with microscope specimen preparation techniques; previous experience in research laboratory environment; familiarity with common windows-based software packages; good interpersonal skills and a knowledge of EEO/AA principles.
Desirable criteria: experience with microscopy of biomedical specimens, experience with cryomicroscopy techniques; ability to use image processing and analysis software. This is a fixed term position to 31 December 2000
Information about the Unit can be found on its website: http://srv.emunit.unsw.edu.au
Enquiries may be directed to Associate Professor Paul Munroe on telephone (02) 9385 4435, facsimile (02) 9385 6400 or email: p.munroe-at-unsw.edu.au.
Applications close 28 January 2000.
Dr. Mel Dickson, Deputy Director, The Electron Microscope Unit, Adjunct Associate Professor, School of Microbiology & Immunology The University of New South Wales Sydney, Australia. Phone +612 9385 6383 Fax +612 9385 6400
I have been looking for some relevant references to put in my PhD thesis which are applicable to the work undertaken.
I was trying to immunogold label (using 5nm gold conjugated to Fab) an epitope on the giant muscle protein titin within muscle fibre bundles (all Ab labelling was done prior to sample fixation). However, the labelling seen was low and inconsistent.
Labelling with FITC conjugated Ab or unconjugated Ab labelled samples which were then stained was fine though.
Does anyone one know of similar work where immunogold labelling has failed and possible reasons for this has been explicitly mentioned within the paper.
I have inherited a Leitz Aristoplan microscope for integration into a digital imaging system for histopathology. Unfortunately, the mid-range objectives (10, 25, 40x) are all fluors, although the scope is not equipped for fluorescence. I would like to acquire planapochromats for each of these magnifications. The Aristoplan is a fixed tube length (160mm) instrument that is excellent optically, but the fluotars cause significant vignetting at all magnifications, even with the correct C mount. If you can provide any or all of these lenses, please contact me off-list with pricing information and purchasing details.
Roger Moretz Dept of Toxicology
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Not having a lot of microscopy experience, I was wondering if anyone could recommend some solutions for effectively clearing Arabidopsis seed coats. I would like to use as non-toxic a solution as possible (no Xylene!) and also one which works quickly. Can anyone give me some handy hints?
Thanks for you help!
Stephen Evans Wheat Improvement Centre Norwich Research Park Colney Norwich NR4 7UH stephen.evans-at-aguk.zeneca.com
Hi Roy: Sorry I haven't gotten back with you sooner, but I was off for the holidays. Do you still need equipment? Give me a call so we can discuss what you need. Regrards, Mike Coviello 817 272-5496
I received this question from a co-worker. Please respond directly to me.
thanks in advance, Marisa Ahmad
--------------------
Small question. We have a Leybold mechanical pump hooked to a Reactive Ion Etcher. It is running 24 hrs/ day, and pumps actively on the chamber about 35 times per day. How often should a pump under these conditions be rebuilt? The reason I am asking is the pump seems to be blowing seals and requires rebuilding about once a year, the manufacturer says this is normal...is it? If not, what should we do to improve the time between rebuilds?
Michael Davidson at Florida State University has an excellent demonstration of the QX3 microscope at: http://microscopy.fsu.edu
Don O'Leary ----- Original Message ----- } From: "Caroline Schooley" {schooley-at-mcn.org} To: {Microscopy-at-Sparc5.Microscopy.Com} Sent: Sunday, January 16, 2000 12:44 PM
11th International Congress of Histochemistry and Cytochemistry (ICHC 2000)
ON-LINE REGISTRATION NOW AVAILABLE
Dear Collegues,
I have mentioned before the upcoming meeting ICHC 2000 3-8 Sept. 2000 York, UK
There will a range of sessions that will I am sure be of great interest to this group. Please take a look at our web-site at http://www.med.ic.ac.uk/external/ichc_2000
Hope to see you their
Best wishes
Gary Coulton Organiser ICHC 2000
Dr. Gary Coulton Molecular Pathology Division of Biomedical Sciences Imperial College School of Medicine The Sir Alexander Fleming Building South Kensington London SW7 2AZ
tel 0044 (0)171 594 3190 fax 0044 (0)171 594 3022
e-mail g.coulton-at-ic.ac.uk
------------------------------------- Announcing the 11th International Congress of Histochemistry and Cytochemistry (ICHC 2000)
"Understanding Biocomplexity: The Post-Genome Challenge"
September 3-8, 2000, York, United Kingdom
ICHC 2000 will comprise 27 symposia addressing the latest developments and applications of histochemistry and cytochemistry in the life sciences including medicine.
Many leading experts to speak
On-line registration now open!!!!!!!!!!
For further details of the meeting and how to pre-register please visit our web-site at http://www.med.ic.ac.uk/external/ichc_2000
} Fellow Microscopists, } } I "found" a Sony Mavigraph UP-D7000 Digital Color Printer with 2 cases } of consumables. What I could not find is any of the software to run the } little beast; and besides it was suppose to go on a Unix system, so } even if I found the software it would be useless {I think}. } } I went to the Sony Web site and found no downloads, any other } suggestions or even some discs etc... would be a great help. BTW, the } Sony will be on a PC. } } Thanks, } } John Grazul } Lucent
Dear Listers, I thought I'd beat Tina to the weather report. Here in Albany, NY (where cryo-microscopy means working with the windows open) we are having Martian summer--yesterday's high was -15 C. Yours, Bill Tivol
Sorry to bug everyone, but I'm trying to reach Robert Derby. If you are out there, please e-mail me! Or if anyone has a contact address for him, could you send it to me?
{/bigger} {/fontfamily} {bigger} {bold} {fontfamily} {param} Times {/param} {bigger} RESEARCH ASSOCIATE
Electron Microscopy Facility
University of Kentucky
{/bigger} {/fontfamily} {/bold} {fontfamily} {param} Times {/param} {bigger} The University of Kentucky invites applications for a Research Associate in the area of electron microscopy. The Electron Microscopy Facility houses two SEMs, two TEMs one AFM and extensive sample preparation equipment that are used by undergraduate and graduate students in support of research and education. The primary responsibility of the Research Associate will be to oversee the JEOL 2010F Field Emission TEM and auxiliary equipment, including an Oxford energy dispersive x-ray spectrometer (EDS), Gatan electron energy loss imaging filter and acquisition hardware and software. S/he will be responsible for coordinating and administering maintenance, access, training and use of the equipment by internal and external users. S/he will also be encouraged to develop sponsored research programs and collaborate with university faculty members. The qualified candidate will have a Ph.D. and practical experience in analytical electron microscopy and demonstrate good communication skills. The salary will be commensurate with qualifications and experience. The University of Kentucky offers comprehensive insurance and benefits packages and is an equal opportunity employer. Please send applications to:
Hi, I am trying to get frozen sections of rat skin about 10 um thick. The tissue was perfusion fixed w/ 4% para in PBS then cryoprotected in sucrose/ PBS and mounted with OTC compound. More often then not my sections are sticking to the knife edge and are hard to remove even with a brush. I'm a bit knew to cryo-sectioning so any tips would be greatly appreciated. Thanks, Andy
} Dear Listers, } I thought I'd beat Tina to the weather report. Here in } Albany, NY (where cryo-microscopy means working with the } windows open) we are having Martian summer--yesterday's } high was -15 C. } Yours, } Bill Tivol
OK, OK, it's in the 70sF, raining with enough sun for spectacular rainbows, and there's a break in the winter North Shore surf season. But I'm stuck in a windowless lab just like the rest of you guys!
http://wavetrak.surfline.com/pipecam.asp
My condolences on your winter blues.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Hello, I have version 1.1 of a plugin for Adobe Photoshop (Macintosh). I can send you a copy to try. You'll be printing "pink" images in no time.
John Bonevich
} john grazul wrote: } } } Fellow Microscopists, } } } } I "found" a Sony Mavigraph UP-D7000 Digital Color Printer with 2 cases } } of consumables. What I could not find is any of the software to run the } } little beast; and besides it was suppose to go on a Unix system, so } } even if I found the software it would be useless {I think}. } } } } I went to the Sony Web site and found no downloads, any other } } suggestions or even some discs etc... would be a great help. BTW, the } } Sony will be on a PC. } } } } Thanks, } } } } John Grazul } } Lucent
-------------------------- John Bonevich, Ph.D. NIST, Metallurgy, Stop 8554 100 Bureau Drive Gaithersburg, MD 20899 USA TEL: (301) 975-5428 FAX: (301) 975-4553
Please don't consider this a "commercial" posting; my goal is to encourage lots of experimentation. The Mattel microscope (http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html )lists for $100, but Toys-R-Us is now selling it for $69.95, in both its retail stores and website. And if you go to www.etoys.com, you can download a Mattel $30 rebate certificate, good till 4/29. So it looks like you can get one for ~$40. Have fun, folks!
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
We have a Micro-g anti-vibration platform that our JEOL 1200EX TEM was sitting on until recently. This allowed us to do decent microscopy on a vibration prone second floor with very good results. We no longer have a need for this unit. It could be used with a TEM or SEM.
What we would like to do is trade it for a new Mac. We can not buy Macs. If you need a platform, let's talk trade. I think that this would be a good deal.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Hi All, We have a Balzars 301 Freeze Fracture with quartz thin film monitor, double replica accessory, and electron guns for both platinum and carbon up for grabs. New seals on the mechanical pump. Original equipment bought circa 1977. Last used 1998, and was perfectly fine. Free, as is. Recipient will pay for moving. Kristen
Kristen A. Lennon Cell, Molecular & Developmental Biology Group Department of Botany & Plant Sciences University of California Riverside, CA 92521 kalen-at-citrus.ucr.edu
Dear Listers, We're investigating both confocal microscopy and epi-fluorescence combined with de-convolution software for a multi-user facility. I'm requesting input from directors and managers of other shared technology laboratories regarding systems chosen and whether the system has met their expectations. Please include websites which include FAQs and clarification of terms. Thanks in advance for your input. Rosemary Walsh EM Facility for the Life Sciences Life Science Consortium & Biotechnology Institute Penn State University University Park, PA. 16802 (814) 865-0212
Dear ALL, there is a lot of good news about the QX3 microscope (toy-)attachment, and I would like to try it under Windows95 or Windows NT4. Is there any driver for this systems? any thanks Lajos Pogany
I have the task to analyse (by EELS technique) precipitates in steel. I would greatly appreciate some hints coming from people who are already familiar with the difficulties connected with: 1. carbon analysis. It is obvius that a carbon film support for the precipitates extracted from the steel is to be avoided. Has anyone experince on the use of other kind of supporting film (silicium monoxide ?, beryllium ?). I have the same problem with using copper grids, because the precipitates are supposed to contain copper too. What kind of grids is most appropriate?
2. would it be a better solution to perform precipitate analysis on thinned steel specimens ? Can it occurr an annoying interference originating in the material surrounding the precipitate ?
3. last but not least, I would greatly appreciate some bibliographical hints on that very specific topics.
Recently, an interesting medical school project has appeared at my lab door. I've been asked to quantify Ca and P in scar tissue found in sections of hamster hearts. Obtaining appropriate standards is critical. Are there commercially available biological standards or procedures to create such standards out there? Any suggestions would be appreciated and thanks for your time.
Dan
===================================================== Dan Kremser Washington University Department of Earth and Planetary Sciences/Campus Box 1169 (Wilson Hall, Room 108-----for packages) One Brookings Dr. St. Louis, MOĘ 63130-4899
Dear Colleagues What is the best glue for making cross-section samples to study ~ 30 micron reaction layer formed by a coating on a Ni-base superalloy? The M-bond 610 which used to work great for Si and ceramic samples does not seem to work here. Though the glue I have is pretty old. TIA Anita
Anyone have any recommendations for organizing digital images and associated data in electronic form in a GLP environment? I'm going crazy trying to find something that will be easy to use, will link the image with associated data information, and will have restriction or audit capabilities to track any changes to the data that may be made.
Any input is greatly appreciated.
Gregg Sobocinski Parke-Davis Pharmaceuticals Ann Arbor, Michigan USA Gregg.Sobocinski-at-wl.com
} What about the Mac? :-) } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
John Mansfield PhD CPhys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555 (Leaving a phone message at 936-3352 is preferable to 358-7555) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42Ą 16' 48" Long. 83Ą 43' 48"
I need to make some ~100nm thick oxide layers on silicon to align an ion gun in an auger system. I assume that most people use thermal oxidation to grow the films.
Does anyone have a recipe for making these films? (time, temperature, atmosphere) It would be really great if the recipe allowed me to get an exact thickness too!
Thanks, Henk
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://web.ceof.ohio-state.edu An optimist believes that we live in the best of all possible worlds. A pessimist fears that this is true.
According to the data sheet packed with M-Bond 610, the room temp pot life, after mixing, is six weeks.
We note that it fails quickly, i.e. it works fine one day and doesn't the next. As we can't abide samples coming unglued, we toss mixed M-Bond 610 after 30 days and mix fresh.
Also, M-Bond 610 works best bonding smooth surfaces. If we have rough surfaces we use GATAN G-1 (Epo-Tec 353ND).
Ron
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
IBM Analytical Services; http://www.chips.ibm.com/services/asg
Anita Garg {Anita.Garg-at-lerc.nasa.gov} on 01/20/2000 10:51:15 AM
To: Microscopy-at-sparc5.microscopy.com cc:
Dear Colleagues What is the best glue for making cross-section samples to study ~ 30 micron reaction layer formed by a coating on a Ni-base superalloy? The M-bond 610 which used to work great for Si and ceramic samples does not seem to work here. Though the glue I have is pretty old. TIA Anita
It's raining cats and dogs, and it's cold and miserable. Feel better, now?
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Does anyone have technical manuals or documentation for a B&L Research II Metallograph which I could copy. MCCC just received one as a donation. Thanks!
Michael Mohn Assistant Professor of Materials Technology Monroe County Community College Phone: 734-384-4122 Fax: 734-242-9711 http://www.monroe.cc.mi.us/mmohn
} } Aloha, } Tina } } It's raining cats and dogs, and it's cold and miserable. Feel better, } now? } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } ****************************************************************************
But what you call cold and what we call cold are two very different animals! Its been snowing all day in the Northeast, and the temps haven't nosed above (or even near) freezing) in days!
Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Confocal Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
I attempted to do this many years ago. I made replicas using aluminium. This was written up in the proceedings of a conference on microanalysis ("Measurement of carbon in V(C,N) precipitates extracted from HSLA steels in aluminum replicas," by A.J. Garratt-Reed, in "Quantitative Microanalysis with High Spatial Resolution," The Metals Society, London, Book No. 277, 1981, p. 165.) Then someone else tried to replicate my work (for the moment I can't remember his name, but he was at Strathclyde University in Glasgow) and could not get consistent results. He concluded that the aluminum was somehow catalysing the oxidation of the carbon in the carbides. I expect he published the results, but I can't now remember where. I have some memory that he used silicon monoxide to make replicas, but when I tried that, the replicas would break up because of beam-induced charging. Of course, it would have defeated the point to have put a carbon coat on the films!
When copper has been an issue, I have often used nickel, which are very satisfactory. You can also get grids of many other materials, including Ti, Al, Au, Mo, etc., etc. - check your EM suppies catalogues.
Tony G-R.
At 10:33 AM 01/20/2000 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
** Anthony J. Garratt-Reed ** MIT Room # 13-1027 ** 77 Massachusetts Avenue ** Cambridge, MA 02139-4307 ** USA ** ** Phone: (+) 1-617-253-4622 ** Fax: (+) 1-617-258-6479 **
Henk, Ta is usually used for measuring sputter rates and you can see where the beam was put. You electrochemically grow a specific thickness and measure the sputter rate. If you want to pursue this, I can give you a referral to a couple of surface scientists who have done this. They are in your neck of the woods.
Another thing that you might want to try is something that I wrote up a number of years ago in JVST as a shop note for aligning an ion gun system where I could not see the beam. Take Double sticky tape and put it on your sample holder. It works in a UHV system well enough, trust me. Then push the sample holder into yellow WO3 powder to cover the sticky tape. Where the beam hits the sample, the WO3 will turn blue. A couple of sample transfers and you have it.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Hendrik O. Colijn [mailto:colijn.1-at-osu.edu] } Sent: Thursday, January 20, 2000 12:54 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: MAT: making silicon oxide layers } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Hi all, } } I need to make some ~100nm thick oxide layers on silicon to } align an ion } gun in an auger system. I assume that most people use } thermal oxidation to } grow the films. } } Does anyone have a recipe for making these films? (time, } temperature, } atmosphere) It would be really great if the recipe allowed } me to get an } exact thickness too! } } Thanks, } Henk } } Hendrik O. Colijn colijn.1-at-osu.edu } Campus Electron Optics Facility Ohio State University } (614) 292-0674 http://web.ceof.ohio-state.edu } An optimist believes that we live in the best of all possible worlds. } A pessimist fears that this is true. } }
I read Tony's answer to your question and I can't add anything to that. However, I just have a minor point. If you are using EELS, why are you worried about the grids? The grids would not show up in the EELS spectrum. Of course it would interfere if you are using EDS.
You can get your grids in almost any material you want nowadays. Ni, Mo, Be, Al, Cu, C, etc. Just pick one that doesn't interfere with your EDS spectrum.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be] } Sent: Thursday, January 20, 2000 4:33 AM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: steel precipitates analysis by EELS } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } -------------------------------------------------------------- } ---------. } } } Dear fellow microscopists, } } I have the task to analyse (by EELS technique) precipitates in steel. } I would greatly appreciate some hints coming from people who are } already familiar with the difficulties connected with: } 1. carbon analysis. It is obvius that a carbon film support for the } precipitates extracted from the steel is to be avoided. Has anyone } experince on the use of other kind of supporting film } (silicium monoxide ?, beryllium ?). I have the same problem with } using copper grids, because the precipitates are supposed to } contain copper too. What kind of grids is most appropriate? } } 2. would it be a better solution to perform precipitate analysis on } thinned steel specimens ? Can it occurr an annoying interference } originating in the material surrounding the precipitate ? } } 3. last but not least, I would greatly appreciate some bibliographical } hints on that very specific topics. } } Thank you in advance. } } Corneliu Sarbu } MTM Dept. of KULeuven, Belgium }
I am celating information about current TEM and Confocal technologies with the intention of setting up both a confocal suite and an EM suite at a new facility. However, I am new to this field and would like some advice on the pros. and cons. of different systems based on other user experiences, to give me an idea of what I should be looking out for. Specifically, the confocal suite would allow the study of both live (EGFP) and fixed samples. Thus, the type of confocal needed should have good resolution for both live and fixed samples as well as good phase contrast. Importantly, this will probably be a multi-user facility, which needs reliable lasers that do not need to be realigned often. It would also be important to have good quality microscope, filters and objectives as well as extras such as a heated stage, video and CCD cameras, appropriate computer workstations and software. Any advice that you could offer on such equipment would be very much appreciated.
The results of the recent election of officers to the Society are now official The current list of elected officers is below.
The new officers elected this year are:
President-Elect RON ANDERSON
Secretary JANET H. WOODWARD (2000-2002)
Director, Physical Sciences THOMAS F. KELLY (2000-2002)
Director, Biological Sciences SARA E. MILLER (2000-2002)
------------------------------------------- The complete list of officers is given below and on the MSA WWW site http://www.msa.microscopy.com/MSADocs/MSAOfficers.html --------------------------------------------
MSA COUNCIL 2000
President KEN DOWNING 326 Donner Lab Lawrence Berkeley Lab Berkeley, CA 94720 (510) 486-5941; Fax (510) 486-6488 Email: khdowning-at-lbl.gov
President-Elect RON ANDERSON IBM Analytical Services IBM Zip-41E Hopewell Junction, NY 12533 (914) 892-2225; Fax (914) 892-2555 E-mail: ron-anderson-at-vnet.ibm.com
Past-President DAVID JOY Rm.232 Science and Engineering Research Facility Univ. of Tennessee Knoxville, TN 37996-0810 (865) 974-3642, Fax (865) 974-9449 and Rm. S189, High Temperature Materials Laboratory Oak Ridge National Laboratory Oak Ridge, TN 37831-6064 (865) 574-6799 E-mail: djoy-at- utk.edu
Secretary JANET H. WOODWARD (2000-2002) Buckman Laboratories, Inc. 1256 N. McLean Blvd. Memphis, TN 38108-1241 (901) 272-6408; Fax (901) 272-6451 E-mail: jhwoodward-at-buckman.com
Treasurer KATHI ALEXANDER (1999-2001) Los Alamos National Lab MST-8 G755 P. O. Box 1663 Los Alamos, NM 87545 (505) 665-4750, Fax (505) 667-8021 Email: kbalexander-at-lanl.gov
Directors, Physical Sciences
J. MURRAY GIBSON (1998-2000) Argonne National Laboratory Materials Science Division Argonne, Ill 60439 {p} Email:gibson-at-anl.gov
THOMAS F. KELLY (2000-2002) 2021 Chamberlain Ave. Madison, WI 53705-4076 (608) 263-1073; Fax (608) 262-8353 E-mail: tfkelly-at-engr.wisc.edu
MIKE KERSKER (1999-2001) JEOL USA 11 Dearborn Rd Peabody MA 01960 (508) 535-5900 Fax (508) 536-2205 E-mail: kersker-at-jeol.com
Directors, Biological Sciences SARA E. MILLER (2000-2002) Duke Medical Center, Pathology Box 3712 Durham, NC 27710 (919) 684-3452; Fax (919) 684-8735 E-mail: saram-at-acpub.duke.edu
AVRIL SOMLYO (1998-2000) Dept. of Molecular Physiol. & Bio. Phys. Box 10011, Health Sciences Center Univ. of Virginia Charlottesville, VA 22906-0011 (804) 982-0825; Fax (804) 982-1616 E-mail: avs5u-at-virginia.edu
JOHN BOZZOLA (1999-2001) EM Ctr - Mailcode 4402 Southern Illinois Univ Carbondale IL 62901 (618) 453-3730, Fax (618) 453-2665 E-mail: bozzola-at-siu.edu
Director, Local Affiliated Societies EV OSTEN (2000-2002) 3M Company 3M Center, Bldg. 201-BE-16 St. Paul, MN 55144-1000 (651) 736-0104; Fax (651) 733-0648 E-mail: efosten-at-mmm.com
============================================== Nestor Your Friendly Neighborhood SysOp
I was wondering if anyone could tell me how to properly install a reticle in an Olympus microscope eyepiece. The eyepiece housing does not seem to come apart, although there are two tiny round holes on either site of the lens. Is there a special tool needed?
I have to admit that until I read Scott's posting, I had missed the point about the elemental interference from the grid not being a problem with EELS analysis.
However, in the case of copper, there is another effect to worry about when making replicas, if the process involves any sort of flotation on, or picking the sample up from, water. Because of its electronegativity, copper can dissolve in the water and then ion-exchange with other elements from the sample, replacing them. Then you really do have copper in the sample. The problem isn't particularly severe with extraction replicas from steels (but then, I have never used extraction replicas from steels to try to analyze for small amounts of copper in the precipitates), but has been terrible, for example, when looking at iron sulphides, which actually had a visible shell of copper sulphide (but I was also fishing those samples out of brine, not distilled water!). The problem doesn't seem to occur with nickel grids, which I use in preference to copper for this type of application, just to be on the safe side.
BTW, I'm glad you got my posting, Scott - I haven't seen it come back to myself yet. I guess the poor mail redirector gets indigestion with the volume it has to deal with!
Tony.
** Anthony J. Garratt-Reed ** MIT Room # 13-1027 ** 77 Massachusetts Avenue ** Cambridge, MA 02139-4307 ** USA ** ** Phone: (+) 1-617-253-4622 ** Fax: (+) 1-617-258-6479 **
************************************************ Robert J. Derby New Mexico Institute of Technology Socorro, N.M. Phone - 505-835-5866 E-mail - rjderby-at-excite.com ************************************************ I have heard through the grapevine that Tamara Howard from Cold Spring Harbor is looking for me. I was told it was posted here but I never got it. Tamara call me at 505-835-5866(Iam 2 hours behind you) Robert
_______________________________________________________ Get 100% FREE Internet Access powered by Excite Visit http://freeworld.excite.com
Just a quick question, I was wondering if anyone had ever tried using a microwave oven to harden their resin??? And if so what sort of results were achieved. Another question I have is how to remove / dissolve hardened resin from an aluminium surface???
I would appreciate any suggestions or advice. Thank you
Many thanks to all who responded on and off-line to my request for information. I hope I acknowledged each one individually.
My quest was to find the real reason for lowering the exposure limit. I won't summarise the replies but: (1) pulmonologists at one hospital are thinking that long term, low level exposure to formaldehyde and glutaraldehyde may cause idiopathic pulmonary fibrosis, (2) I was told of a serious skin burn caused by a splash.
I have now heard from the UK Health & Safety Commission's Advisory Committee on the Toxicity of Substances (ACTS). I have copy from: HSE Review 1997, published 1999, section C58 (consisting of 4 pages). Quotes from this are below.
The official reason for lowering the exposure limit is that it has not been possible to set a no-observed adverse effect level for glutaraldehyde with regard to the induction of asthma.
Carcinogenicity is not supported by the available good-quality evidence to date. ________________________________________
Glutaraldehyde must be labelled under the Chemicals (Hazard Information and Packaging for supply) Regulations 1994 (CHIP) as Toxic, Corrosive, Sensitising and Dangerous for the Environment.
Several thousand tonnes are imported into the UK each year. It is primarily used as a biocide and disinfectant in the health care, off-shore, paper-making and agricultural sectors.
.. it is estimated that a considerable number of (health care) workers are intermittently exposed, given the widespread use in that sector. Similarly, ....... for several hundred workers in the manufacture of glutaraldehyde solutions.
..... available exposure data relates mainly to use in the health care sector....... suggests that under normal operational conditions short term exposures are generally less than 0.2 ppm. This can be exceeded during the cleaning of endoscopes ...... or the wiping of surfaces.
HEALTH EFFECTS - ANIMAL STUDIES. Glutaraldehyde is acutely toxic to rats by inhalation, oral and dermal routes. The principal effects are due to its irritant properties.
Glutaraldehyde is clearly a skin sensitiser in rabbits, guinea pigs, rats and mice.
Glutaraldehyde is clearly mutagenic in vitro, in bacterial and mammalian cells. ............. No firm conclusions can be drawn from the available evidence on chromosomal aberrations, but glutaraldehyde clearly causes sister chromatid exchange (SCE) and unscheduled DNA synthesis (UDS) in mammalian cells.
......... However, the clearly negative results of recent, good-quality bone marrow cytogenetics and peripheral blood micronucleus tests, together with those of the liver UDS assay, provide reassurance that the genotoxic effects shown in vitro are unlikely to be expressed in vivo.
No reports of carcinogenicity studies of glutaraldehyde by the inhalation or dermal routes of administration are currently available. A recent oral study provided no convincing evidence ... in rats ..... drinking water for up to two years.
No significant effects on reproduction were reported in a modern two generation study ........... There were no indications of significant gonadal effects in 13 weeks inhalation studies carried out in rats or mice, or in a lethal assay in mice.
HUMAN DATA Glutaraldehyde is irritant to to human skin at concentrations of 2-10%, but not 0.5%. Higher concentrations have not been investigated.
There is substantial evidence that glutaraldehyde is a skin sensitiser in humans. Concentrations as low as 0.13% have induced allergic contact dermatitis. The majority of cases have been reported in health or funeral workers.
A fair body of evidence ............. indicates that glutaraldehyde has the potential to cause occupational asthma.
.......... several workplace studies with exposure data in which no cases of asthma have been found among contemporary workers. ..... However, superimposed on these data are other reports of sensory irritation and / or asthma in endoscopy nurses where the reported levels of exposure overlap with those in the above studies. .................. From the data available, it has not been possible to determine a NOAEL (no-observed adverse effect level) for the induction of asthma.
No information is available in genotoxicity in humans.
The only available mortality study is of limited value, but does not provide any evidence that glutaraldehyde caused cancer or increased mortality in glutaraldehyde production workers.
On the basis of the very limited information available, it does not appear that glutaraldehyde causes reproductive toxicity in humans.
REFERENCE: Glutaraldehyde: Criteria document for an occupational exposure limit EH/65/32. HSE Books ISBN 0 7176 1443 3. _________________________________________________
Dr. Keith Ryan Marine Biological Association of the UK The Laboratory Citadel Hill Plymouth Devon PL1 2PB England
Tel. ++44 (0)1752 633249 Tel. ++44 (0)1752 633279 The 279 number has an answering machine
Fax ++44 (0)1752 633102
e-mail: kpr-at-wpo.nerc.ac.uk
PS - Daniele, you're still here! Keep smiling!! That was a nice evening in Strasbourg. Do you know which Gewurztraminer we all had as an aperatif? Now the world will wonder?!
************************************************ Robert J. Derby New Mexico Institute of Technology Socorro, N.M. Phone - 505-835-5866 E-mail - rjderby-at-excite.com ************************************************ Does anyone or any company know of a ccd that will capture single photon at the 852-872 wavelength? This is needed for a special project. Any help would be of great help Thanks,
Robert
_______________________________________________________ Get 100% FREE Internet Access powered by Excite Visit http://freeworld.excite.com
Treasurer KATHI ALEXANDER (1999-2001) Los Alamos National Lab MST-8 G755 P. O. Box 1663 Los Alamos, NM 87545 (505) 665-4750, Fax (505) 667-8021 Email: kbalexander-at-lanl.gov
Directors, Physical Sciences
J. MURRAY GIBSON (1998-2000) Argonne National Laboratory Materials Science Division Argonne, Ill 60439 {p} Email:gibson-at-anl.gov
THOMAS F. KELLY (2000-2002) 2021 Chamberlain Ave. Madison, WI 53705-4076 (608) 263-1073; Fax (608) 262-8353 E-mail: tfkelly-at-engr.wisc.edu
MIKE KERSKER (1999-2001) JEOL USA 11 Dearborn Rd Peabody MA 01960 (508) 535-5900 Fax (508) 536-2205 E-mail: kersker-at-jeol.com
Directors, Biological Sciences SARA E. MILLER (2000-2002) Duke Medical Center, Pathology Box 3712 Durham, NC 27710 (919) 684-3452; Fax (919) 684-8735 E-mail: saram-at-acpub.duke.edu
AVRIL SOMLYO (1998-2000) Dept. of Molecular Physiol. & Bio. Phys. Box 10011, Health Sciences Center Univ. of Virginia Charlottesville, VA 22906-0011 (804) 982-0825; Fax (804) 982-1616 E-mail: avs5u-at-virginia.edu
JOHN BOZZOLA (1999-2001) EM Ctr - Mailcode 4402 Southern Illinois Univ Carbondale IL 62901 (618) 453-3730, Fax (618) 453-2665 E-mail: bozzola-at-siu.edu
Director, Local Affiliated Societies EV OSTEN (2000-2002) 3M Company 3M Center, Bldg. 201-BE-16 St. Paul, MN 55144-1000 (651) 736-0104; Fax (651) 733-0648 E-mail: efosten-at-mmm.com
============================================== Nestor Your Friendly Neighborhood SysOp
--CAB19553.948442177/styx.services.ou.edu--
} From MAILER-DAEMON Fri Jan 21 02:09 CST 2000 Received: from localhost (localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with internal id CAA00349; Fri, 21 Jan 2000 02:09:34 -0600
Hello, all-
I'm hoping to hear from those of you who have worked out the optimum size and resolution to make images in e.g., Photoshop that are destined for PowerPoint to be made into transparencies. An image that is 4 x 5 inches and 400-600 dpi seems to be overkill for a 35mm slide.
Your opinions?
Mahalo, Tina **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
I found the following recipe from Bernie Kestel. He states that this is used for surface polishing using the beaker method. Although he did not use it for "jet polishing", it may be a good starting point.
Inconel 718 (annealed) 10% HClO4 90% Ethanol Temperature = -60C Current: 275mA Volts: as required
There is some more information about stir rate, sample orientation etc. that is only relevant for the beaker method. If you'd like to try this approach, I would be happy to send you the entire reference.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by Schryvers Dominique } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I'm looking for a good electropolishing solution + conditions for as-received and annealed Inconel 718 for use with a Tenupol 3 system to produce well thinned matrix + precipitates for TEM work. Any suggestions?
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
A minor observation ...........
While the issue of overlaps is generally not a problem with EELS (as opposed to EDS), if can arise with steel precipates. The vanadium L and Oxygen K edges are really quite close, so if you have vanadium precipatates, a silicon oxide support film will cause problems for quantitation.
regards, -- Larry Stoter 17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, UK email: LPS-at-teknesis.demon.co.uk, Home Phone/Fax: +44 (0)1825 767967
Yikes! Great! set the microscope near the window and get those snow flake pictures! It has been close to 80 degrees a few days this week here in Arizona! I have a great batch of infusion brewing on the back patio.
Got the happage win TV card to add to the computer....Have the c-mount ccd camera I found for $100.... now... I should be able to share pictures soon!
Ed Sharpe archivist for SMECC
{ { Subj: Martian summer Date: 1/22/00 7:50:37 AM Pacific Standard Time From: tivol-at-wadsworth.org (William Tivol) Sender: tivol-at-wadsworth.org To: microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
Dear Listers, I thought I'd beat Tina to the weather report. Here in Albany, NY (where cryo-microscopy means working with the windows open) we are having Martian summer--yesterday's high was -15 C. Yours, Bill Tivol } }
The main Microscopy Server was replaced this weekend (read that as many blurry eyed hours in front of a monitor, and lots of cursing at new formats of configuration files for DNS and SENDMAIL).
The old beast was growing increasing unreliable with hardware crashes nearly daily. I believe that most of the services have been restored however until all databases are reconfigured and tested there will be some glitches. Please be patient.
I think I have been able to capture the few messages that were sent over the weekend. If those of you that posted items over the weekend don't see things in the next day please repost them.
Dear Tina, Our medical photography dept. requests that files submitted for slides are at least 1 Mb and no more than 3 Mb in size. Less than this doesn't have enough pixels to adequately fill the image (similar to a thin negative), and more is unneeded. I create the slide figure, set to 300 dpi and adjust dimensions to put the file size into that range.
Regards, Glen
Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
On Fri, 21 Jan 2000, Tina Carvalho wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, all- } } I'm hoping to hear from those of you who have worked out the optimum size } and resolution to make images in e.g., Photoshop that are destined for } PowerPoint to be made into transparencies. An image that is 4 x 5 inches } and 400-600 dpi seems to be overkill for a 35mm slide. } } Your opinions? } } Mahalo, } Tina } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } }
Position: Full/Associate/Assistant Professor of Materials Science and Engineering. This is a full time, tenure track teaching and research position beginning Fall 2000.
Rank and Salary: Rank will depend upon background and experience; salary competitive.
Responsibilities: Teach undergraduate and graduate courses in materials science and engineering, advise undergraduate and graduate students, seek and conduct funded research, supervise theses and projects, and serve on university, college and department committees.
Academic Qualifications: Earned Ph.D. in materials science and/or engineering or a closely related field is required. Demonstrated experience in research and grant seeking is highly desired. Prior teaching experience a plus.
Experience Qualifications: Demonstrated experience in research and grant seeking is highly desired. Prior teaching experience a plus.
Department: The Department of Construction Engineering, Materials Engineering and Industrial Design at Western Michigan University currently offers two undergraduate BSE -- Materials Engineering and Construction Engineering and Management, and BS in Industrial Design. The Department also offers two Master of Science programs - Materials Science and Engineering and Construction Management.
University: Western Michigan University, with a student body of approximately 28,000 is located in southwest Michigan. Kalamazoo is halfway between Chicago and Detroit and 45 miles south of Grand Rapids. The population of the greater Kalamazoo area is approximately 200,000. Its industry is highly diversified and it is the center of many cultural and sporting events.
Applications: Review of applications will begin on January 10, 2000, and will continue until the position is filled. Please send the following credentials: Letter of Application addressing qualifications, Vitae, Transcripts from all institutions, and the names/addresses, telephone/fax numbers of three references
Contact: Please send credentials to:
Dr. Roman Rabiej, Chair Western Michigan University Department of Construction, Materials, & Industrial Design 2007 Kohrman Hall Kalamazoo, MI 49008
AN EQUAL OPPORTUNITY/AFFIRMATIVE ACTION EMPLOYER Western Michigan University is an Equal Opportunity Employer. In addition, it has embarked upon a vigorous affirmative action program and encourages the applications of women and members of minority groups. ============================== Pnina Ari-Gur, D.Sc., Professor of Materials Science and Engineering Western Michigan University Kalamazoo, MI 49008 (616) 387-3372 FAX: (616) 387-6517 email: pnina.ari-gur-at-wmich.edu http://www.wmich.edu/cmd/arigur.htm ==============================
Does anyone have a Probe Current Detector accessory for a JEOL 840 surplus to requirements and available for purchase? I think its designation is PCD40, it's the pneumatically-operated thing which mounts on the column opposite to the objective aperture holder, and shoots a Faraday cup across into the beam.
thanks
Ritchie Sims
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Dear Dr. Fashing, it is a pleasure receiving news from you. I have to apologize for the trouble you have reported in your letter and I hope to help you.
First of all, the website is going to be updated because of the second circular is ready to be sent.
The second point is that you have enough time to submit your paper or poster. The deadline for submission has been established on 30 April 2000. I hope you answered to the first circular (my database has not been recently updated - Migliorini is working on this and he has the complete and updated archive), so in this case you will receive the second circular directly on your computer. Otherwise I suggest you to fill the application on the web site.
Let me know if I could help you more. I will be glad to do that.
My best wishes and see you in Siena
Enrico
} Dear Dr. De Lillo, } } I am interested in attending the EURACC symposium in Siena this July, } and would greatly appreciate receiving information concerning that } meeting. I keep checking the meeting homepage on the web site } (http://www.unisi.it/ricerca/dip/bio_evol/sitoeuraac/siena2000.html), } but very little information is given. I also contacted the e-mail } address (euraac2000-at-unisi.it) a few months back and have not yet } received a reply. I wrote to Dr. Leo P. S. van der Geest, the EURAAC } Secretary, yesterday and he gave me your e-mail address and thought } perhaps you could help. } } I would like to present a paper at the meeting (probably a poster) } and need to know when titles and abstracts are due and to whom they } should be sent. Also the format that should be used for submitting } the paper. } } Many thanks for your help; it is greatly appreciated. I look forward } to hearing from you. } } Sincerely, } } Norm } } Norm Fashing } Professor of Biology } Department of Biology } College of William and Mary } P.O. Box 8795 } Williamsburg, VA 23187-8795 } 757 221-2221 (Office) } 757 221-6483 (FAX) } njfash-at-facstaff.wm.edu } http://www.wm.edu/biology/Fashing.html
dr Enrico de Lillo Istituto di Entomologia agraria - Universit Bari - Italy via Amendola, 165/A - 70126 Bari - Italy tel. +39 080 5443105 fax +39 080 5442876 email: delillo-at-agr.uniba.it http://193.204.185.103/de_lillo.htm
The power supply in our cryo microtome is having problems which might be related to the transformer. I was told by the service engineer that the transformer is no longer supported by Reichert. Does any one know where I can get a replacement ?
Dear Nestor, Thanks for taking such good care of us. Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Confocal Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Anyone have any recommendations for organizing digital images and associated data in electronic form in a GLP environment? I'm going crazy trying to find something that will be easy to use, will link the image with associated data information, and will have restriction or audit capabilities to track any changes to the data that may be made.
Any input is greatly appreciated.
Gregg Sobocinski Parke-Davis Pharmaceuticals Ann Arbor, Michigan USA Gregg.Sobocinski-at-wl.com
Contact Robin Griffin at UAB. I bought that unit there and we worked on 718 and developed the polishing conditions for it. I believe that we used the butyl cellusolve(SP?)/perchloric acid solution at low temp to do it. She should have the recipe or know who to contact. Her Email address is rgriffin-at-eng.uab.edu or you might get a hold of Ray Thompson whose student did the work. As I recall, the as-cast material was very difficult to do and had very narrow conditions. The annealed samples were a little easier. To save time, we had the samples initially cut out of the bulk samples using an EDM machine.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Schryvers Dominique [mailto:schryver-at-ruca.ua.ac.be] } Sent: Friday, January 21, 2000 2:53 AM } To: Microscopy MAIL } Subject: Inconel 718 polish } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } -------------------------------------------------------------- } ---------. } } } I'm looking for a good electropolishing solution + conditions for } as-received and annealed Inconel 718 for use with a Tenupol 3 } system to } produce well thinned matrix + precipitates for TEM work. Any } suggestions? } } Nick Schryvers } } } *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=* } *=* *=* } *=* Dr. D. Schryvers *=* } *=* Electron Microscopy for Materials Research (EMAT) *=* } *=* University of Antwerp, RUCA *=* } *=* Groenenborgerlaan 171 *=* } *=* B-2020 ANTWERP *=* } *=* Belgium *=* } *=* tel: 32-3-2180247 *=* } *=* fax: 32-3-2180257 *=* } *=* e-mail: schryver-at-ruca.ua.ac.be *=* } *=* homepage: http://www.ruca.ua.ac.be/EMAT *=* } *=* *=* } *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=* } }
The short answer to you question would be an image of about 1024 pixels across should be adequate for a slide used for a presentation. The slide will appear to most people as a 8x10"-print held at arm's length, or as a computer screen at 4 to 6 feet. If you cannot make out the pixels in those images, then you probably have enough pixels in your image for PowerPoint.
I think I start seeing the pixels when the resolution drops to 800 or 640 pixels across. I might see a benefit in raising the pixels to 1280 across, but I am not able to see improvement beyond that point. This would give you an image of about 1 million pixels.
Now if you are shooting your slide with a 35-mm camera, it might be good to use 24-bit color on the image if your output device (e.g., printer) can well render it. However, since the slide is only for a presentation, you can probably get by with much less color depth if file size per slide is an issue. I venture to say that 8-bit color at 1024 pixels across is plenty adequate for most presentations.
Remember, the above considerations are only for images for slide presentations. If the slides are meant to archive the images for other purposes, then you probably want every bit of resolution and color depth that your technology allows and justifies.
Warren
At 02:18 PM 1/21/2000 -1000, you wrote: } Hello, all- } } I'm hoping to hear from those of you who have worked out the optimum size } and resolution to make images in e.g., Photoshop that are destined for } PowerPoint to be made into transparencies. An image that is 4 x 5 inches } and 400-600 dpi seems to be overkill for a 35mm slide. } } Your opinions? } } Mahalo, } Tina
Periodically questions appear on this list concerning the use of cryostats for cutting histological sections. I usually reply to the sender off line and offer a copy of a handout that I got from a workshop at a Histochem Meeting some years ago. Even though it is old, cryostat sectioning has not changed a lot. I think there is some valuable info there, especially for beginners. I thought it might be helpful to make this available on the net for whomsoever might want to take a look. It can be found at : http://www.biotech.ufl.edu/sems/
Look for the snowflake
It was written by Bruce Quinn, then of MIT. I hope he has no objections to my posting it.
Greg Erdos Gregory W. Erdos, Ph.D. Ph. 352-392-1295 Assistant Director, Biotechnology Program PO Box 110580 Fax: 352-846-0251 University of Florida Gainesville, FL 32611
A full time position is available immediately for a highly motivated individual to work in the Center for C. elegans Anatomy at the Albert Einstein College of Medicine, located in the Bronx, New York. The candidate should have a Bachelor's degree in Biology or some related science, and some previous laboratory experience. We are particularly looking for an individual with training in transmission electron microscopy and thin section microtomy. Experience with immunocytochemistry and/or computerized image analysis is helpful but not required.
The College offers a generous compensation package including 4 weeks vacation and tuition reimbursement. Qualified candidates should submit a resume and a list of references to:
Dr. David Hall Department of Neuroscience Albert Eistein College of Medicine 1410 Pelham Parkway Bronx, NY 10461
Qualifications (education, certification, language, etc.) and Experience required: A candidate with a BS or MS or PHD degree in physical science is preferred. Prior experience in electron probe microanalysis is essential.
Job Overview: The Surface/Microscopy/X-ray (SMX) Group in Dow's Corporate R&D Analytical Science Laboratory has one professional level full time opening for an electron microprobe analyst. The candidate should have theoretical and practical experience in electron beam techniques, including quantitative x-ray microanalysis, digital imaging, digital x-ray imaging, electron beam/solid interactions, scanning electron microscopy and material science. Good computer skills are very desirable. Good written and oral communication skills and the ability to work both independently and in a team environment are extremely important.
Key responsibilities will include: 1. Extensive problem solving on a wide variety of Dow materials and processes 2. Operation and routine maintenance of a CAMECA SX-50 electron microprobe. 3. Some sample preparation including microtomy and metallography 4. Operation of light microscopes. 5. Operation of scanning and transmission microscopes as needed. 6. Interpretation of images. 7. Documentation of work. 8. Compliance with safety and quality systems
Interested: Please e-mail or send your resume and cover letter, with reference to this ad to: Email: R&D-at-Dow.com or The Dow Chemical Company, Workforce Planning, P. O. Box 150, Plaquemine, Louisiana 70765-0150. E-mail respondents must list Job 006145 and their last name as the first and second items on the Subject line. Only those selected for an interview will be contacted. Only U.S. citizens or aliens who are authorized to work in the United States will be considered for employment.
We are an equal opportunity employer and offer a competitive compensation and benefits package including 401k, stock purchase, tuition reimbursement and performance incentives. The Dow Chemical Company is the fifth largest chemical company in the world with annual sales of US$20billion. Dow manufactures and supplies chemicals, plastics and agricultural products for customers in 164 countries and employs approx. 43,000 people worldwide. For more news and information about Dow, please visit our web site at www.dow.com.
Robert C. Cieslinski The Dow Chemical Company Microscopy & Microanalysis (517) 636-6875 email: rccieslinski-at-dow.com
We successfully import our digital images into a PDF file using Adobe Acrobat.
Harry Ekstrom
-----Original Message----- } From: Sobocinski, Gregg [mailto:Gregg.Sobocinski-at-WL.com] Sent: Monday, January 24, 2000 8:35 AM To: 'Microscopy-at-MSA.Microscopy.Com'
Anyone have any recommendations for organizing digital images and associated data in electronic form in a GLP environment? I'm going crazy trying to find something that will be easy to use, will link the image with associated data information, and will have restriction or audit capabilities to track any changes to the data that may be made.
Any input is greatly appreciated.
Gregg Sobocinski Parke-Davis Pharmaceuticals Ann Arbor, Michigan USA Gregg.Sobocinski-at-wl.com
Does anyone or any Company know of a CCD that will do single photon detection at 852 wavelength? This is for a very specialized app. Thanks in advance
************************************************ Robert J. Derby New Mexico Institute of Technology Socorro, N.M. Phone - 505-835-5866 E-mail - rjderby-at-excite.com ************************************************
_______________________________________________________ Get 100% FREE Internet Access powered by Excite Visit http://freeworld.excite.com
first, I would like to wish you all the best for the new year. Now my question: Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?
Is it that delicate as LR-White (Meanwhile I donŦt use LR-W older than half a year). My bottle which was not opened many times, could be roundabout three years old.
Thanks a lot, Michael
Michael Reiner Department of Anatomy I University of Cologne Germany michael.reiner-at-smail.uni-koeln.de
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Dear all,
we're starting a project of HRTEM on small (nanoscale) colouring metal particles in glass. Does anyone have experience with sample preparation for this type of material. We prefer ion-milling as this retains the relative positions of the particles with respect to the surface. Any suggestions, also on literature, are welcome.
Many thanks in advance,
Nick Schryvers
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=* *=* *=* *=* Dr. D. Schryvers *=* *=* Electron Microscopy for Materials Research (EMAT) *=* *=* University of Antwerp, RUCA *=* *=* Groenenborgerlaan 171 *=* *=* B-2020 ANTWERP *=* *=* Belgium *=* *=* tel: 32-3-2180247 *=* *=* fax: 32-3-2180257 *=* *=* e-mail: schryver-at-ruca.ua.ac.be *=* *=* homepage: http://www.ruca.ua.ac.be/EMAT *=* *=* *=* *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id IAA06203 for dist-Microscopy; Tue, 25 Jan 2000 08:53:13 -0600 (CST) Received: from no_more_spam.com (sparc5.microscopy.com [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id IAA06200 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Tue, 25 Jan 2000 08:52:42 -0600 (CST) Received: from snarl.biotech.ufl.edu (snarl.biotech.ufl.edu [128.227.60.109]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id IAA06193 for {Microscopy-at-sparc5.microscopy.com} ; Tue, 25 Jan 2000 08:52:31 -0600 (CST) Received: from empc1 by snarl.biotech.ufl.edu (8.8.5/4.09) id JAA02445; Tue, 25 Jan 2000 09:47:10 -0500 (EST) Message-Id: {4.2.0.58.20000125094650.00a545f0-at-biotech} X-Sender: gwe-at-biotech X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58
Some people have trouble and some do not. It is an Acrobat . After you get the blank screen, try hitting the reload button on your browser menu. This has worked for some people. I need to consult a web expert to see why my PDF files cause trouble.
Nestor, are you out there?????
If you still have trouble, let me know and I will put it into an HTML file. My apologies to anyone having trouble.
Greg Erdos
At 09:39 AM 01/25/2000 -0500, you wrote: } Dear Greg; } I was most interested to look at your tips etc. for cryo sectioning, } but when I clicked on the snowflake, all I ended up with was a blank } screen. Any idea what I did wrong (or is my computer system to blame?) } } thanks in advance } shea } } } } Dr. S. Shea Miller } Agriculture and Agri-Food Canada } Eastern Cereal and Oilseed Research Centre } 2068 K.W. Neatby Bldg } Central Experimental Farm } Ottawa, Ontario } Canada K1A 0C6 } email: millers-at-em.agr.ca } phone: 613-759-1760 } fax: 613-759-1701
Gregory W. Erdos, Ph.D. Ph. 352-392-1295 Assistant Director, Biotechnology Program PO Box 110580 Fax: 352-846-0251 University of Florida Gainesville, FL 32611
Your best source of advice would be Scott Walck, at these contacts:
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
Scott has been in the 'TEM of glass' business for years and has developed a series of techniques relevant to the preparation of cross-sections of this material, which I assume you require when you say, "we prefer ion-milling as this retains the relative positions of the particles with respect to the surface." As Scott will probably tell you, there is a small-angle cleaving technique that you may find preferable to ion milling.
Cheers John
John P. McCaffrey National Research Council of Canada M-50, Montreal Rd. Ottawa, Ontario K1A 0R6 CANADA
-----Original Message----- } From: Schryvers Dominique [mailto:schryver-at-ruca.ua.ac.be] Sent: Tuesday, January 25, 2000 9:51 AM To: Microscopy MAIL
Dear all,
we're starting a project of HRTEM on small (nanoscale) colouring metal particles in glass. Does anyone have experience with sample preparation for this type of material. We prefer ion-milling as this retains the relative positions of the particles with respect to the surface. Any suggestions, also on literature, are welcome.
Many thanks in advance,
Nick Schryvers
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=* *=* *=* *=* Dr. D. Schryvers *=* *=* Electron Microscopy for Materials Research (EMAT) *=* *=* University of Antwerp, RUCA *=* *=* Groenenborgerlaan 171 *=* *=* B-2020 ANTWERP *=* *=* Belgium *=* *=* tel: 32-3-2180247 *=* *=* fax: 32-3-2180257 *=* *=* e-mail: schryver-at-ruca.ua.ac.be *=* *=* homepage: http://www.ruca.ua.ac.be/EMAT *=* *=* *=* *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
we're starting a project of patterning on BG of YBCO films. I got difficulties to find the BG by using opital microscope, because we can not etch the sample before patterning. Does anyone have experience with checking the GB by OM? We appraciate any suggestions or references.
University of Connecticut Institute for Materials Science
Postdoctoral Research Position in Electron Microscopy
The Institute for Materials Science (IMS) at UConn is an interdisciplinary center with the threefold mission of fostering education, research and outreach in all areas of the materials sciences. Due to a re-organization of the IMS Microscopy Unit, a Postdoctoral Position has become available in the area of transmission electron microscopy. The appointee will be involved in a range of academic and industrial projects, and will assist in developing the TEM facilities. Candidates should hold a PhD in Materials Science, Physics or a related discipline and must have extensive hands-on experience in a broad range of electron microscopy techniques. Experience in instrument development and/or computer image processing/simulation would be beneficial. The appointment is for one year in the first instance and is available immediately. Screening of the applications will begin immediately and will continue until the post is filled. Applications from under-represented groups, including minorities, women and people with disabilities are encouraged.
Interested candidates should send a curriculum vitae, including publication list, and the names of at least three referees with postal addresses, telephone numbers and Email addresses to: Prof. M. Aindow, Institute for Materials Science, University of Connecticut, 97 North Eagleville Road, U-3136, Storrs, CT 06269-3136 USA. Email: maindow-at-ims.uconn.edu
I recently asked Michael Davidson of FSU (http://microscopy.fsu.edu/) if he had any success running the QX3 on a Mac w/ USB and he said "It works with a windows 98 emulator, but very very slowly."
Several people have had success running the QX3 with twain drivers from other programs such as Paint Shop Pro and Photoshop (http://clubs.yahoo.com/clubs/qx3). Does anybody know of a twain driver that would run the QX3 from a Mac?
Position Title: (Technical-Level) Scientist-Electron Microscopy
Company: Alcon Laboratories, Inc., a wholly owned subsidiary of Nestle, S.A., has 10,000 employees and worldwide sales of $2.4 billion. It is the global leader in the discovery, development, manufacture and marketing of ophthalmic pharmaceuticals and medical devices. Alcon expects to double its sales over the next five years and has achieved a profit growth rate of approximately 11% over the last several years. Historically, the company commits 10% of sales to research and development. Products developed in the last ten years generate 50% of current sales. The current product pipeline is strong. Alcon was just renamed to the Fortune List of the 100 Best Companies to Work for in America.
Location: Fort Worth was recognized by USA Today as one of the 20 best cities in which to live and work.
Position Responsibilities: The EM Unit is a core resource for R&D, witnessed by the fact that the staff of three generated 9,350 electron micrographs from 1,160 processed specimens in 1998 alone. The successful candidate will be a key member of the EM Unit who processes, examines and provides preliminary interpretation of ophthalmic devices as well as human and animal tissue specimens from a wide variety of R&D groups. S/he will provide electron microscopic research and method development directed towards the discovery of new drug candidates and unique ophthalmic devices, the understanding of pathogenic mechanisms and the identification of new therapeutic agents. S/he will handle the EM Unit commitment to several groups: Surgical-Intraocular Lens, Toxicology, and Degenerative Diseases, as well as contributing to Glaucoma Therapeutic Research, Surgical, Formulations, Consumer Technical Support, Physical Characterization and Pharmacokinetics.
Responsibilities include preparing ophthalmic devices for SEM and x-ray analysis and human and animal tissue for TEM and SEM; use and daily maintenance of Zeiss CEM-902, Cambridge Stereoscan-120 and Zeiss DSM-940, and PGT System 4+ x-ray system; developing and implementing EM techniques for various research projects; assisting with human and animal tissue procedures; and providing preliminary interpretation of EM data.
Preferred Qualifications: Candidates should possess a Bachelor of Science degree in a related discipline plus at least seven years of significant EM experience related exclusively to human and animal tissue. Collaborative and problem solving skills are essential for this position. The successful candidate will also demonstrate highly refined interpersonal and technical writing skills. Certification by or eligibility to be certified by the MSA is a plus.
Alcon professionals enjoy state-of-the-art facilities in a year-round business casual environment. Our company offers competitive salaries and a wide array of excellent benefits: a very generous retirement plan and dollar-for-dollar matching (up to 5%) 401K, medical, dental, vision, life, and accident insurance, death, dismemberment, illness and disability benefits, tuition reimbursement, employee credit union, adoption assistance, dependent care, and wellness programs, on-site fitness center, running track, cafeteria, and company store, innovative paid time off and holidays, and retiree medical coverage.
An Equal Opportunity and Affirmative Action Employer. Pre-employment drug testing.
Please email your resume and salary requirements to: Job28_1261-at-careers.alconlabs.com Reference Code: EM
Apparently several were unable to read the cryostat technique pdf file that Dr. Greg Erdos had generously posted at his website. The answer to the problem could be the version of Acrobat used. I had the "blank page" problem with version 3.0 but no problem at all with version 4.0 (Mac).
} } Aloha, } Tina } } It's raining cats and dogs, and it's cold and miserable. Feel better, } now? }
But what you call cold and what we call cold are two very different animals! Its been snowing all day in the Northeast, and the temps haven't nosed above (or even near) freezing) in days!
Dear Lee, Fair is fair. Often wind and rain at slightly above freezing is more miserable than snow at a few degrees below--having been both in San Francisco for the first condition and Albany NY for the second, I can say this with first-hand authority. Yours, Bill Tivol
Appropriate vendors, I have a Kevex detector which has been giving peak widths larger than specs. It probably just needs an overhaul, but there may be some repair necessary for the pre-amp and FET. Could anyone who can undertake this please respond to me off-list with estimates for various contingencies? TIA. Yours, Bill Tivol
Our Ohio company is seeking an engineer / scientist to research the relationships between the chemistry and microstructure of solid lubricant and hard coatings and their performance in the lubrication of aerospace systems. Research will involve a variety of surface analytical tools (XPS, Raman, etc.) so that fundamental mechanisms of lubrication can be elucidated. Emphasis on microstructure will require expertise with TEM and SEM including preparation of SEM & TEM specimens of thin films and wear scars on steel and ceramic substrates. Research will also involve correlating thin film properties with deposition plasma characteristics and making recommendations for improving lifetime and performance of such materials in different environments: e.g., vacuum, moist air, high temperature, etc.
It is important that candidates have capabilities in cross-section TEM, analytical TEM, analysis of unique microstructures; and understand TEM of thin films on a fundamental level. It is desirable that the candidate have knowledge of tribological materials and experience with TEM/XTEM of wear tracks.
Contact Ronald Decker - mailto:decker-at-utcdayton.com
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} Ronald C. Decker Program Manager Universal Technology Corporation 1270 N FAIRFIELD RD DAYTON OH 45432-2600
Voice (937) 426-8530, Fax (937) 426-7753 (Voice mail is available at my extension, 270)
While browsing news from the latest MacWorld Expo I came across a brief comment about a USB video microscope for the Mac. Further searches at the MacWorld Expo website or at Apple's web site have not turned up anything more about it, although there were announcements that Data Translation and National instruments have released additional PCI and USB I/O boards for the Mac. Has anyone heard more of this?
Regards, Glen
Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
High Resolution Scanning Electron Microscopist/Engineer
United Technologies Research Center is seeking an engineer to fill the HR-SEM operator/engineer position at the United Technologies Research Center in East Hartford, CT. This position will provide support to the United Technologies Corporation Business Unites including Pratt & Whitney, Carrier, Sikorsky, Hamilton Sundstrand and Otis. The SEM operator/engineer will be responsible for the full utilization of both high-resolution secondary and back-scattered imaging to characterize a wide range of metallic and non-metallic materials, including surface coatings and advanced structural materials including metals and ceramics. In addition, the ability to recognize fracture modes and origins of fractures is strongly desired. The candidate should be experienced in the use of EDS for both qualitative and quantitative analyses, including compositional mapping and line profiles. The qualified candidate must be capable of judging the optimal combinations of imaging and EDS to yield t! he most informative characterization of a particular specimen. Good communication and interpersonal skills are essential. Experience with electron backscatter diffraction (EBSD) is a plus.
Qualified candidates will have BS in Materials Science or an equivalent discipline, with a minimum of 2 years SEM experience. U.S. citizenship or permanent resident status is required.
Please visit our web site at http://www.utrc.utc.com for additional general information. Interested parties should send a letter of application and a resume to Employment Opportunities, Code MATS-2050-9049, United Technologies Research Center, 411 Silver Lane, East Hartford, CT 06108 or e-mail employment-at-utrc.utc.com. United Technologies Research Center is an equal opportunity employer.
I tried to open the file with Acrobat 4.0 in Windows 98. No luck.
Damian Neuberger etc., etc.
} Apparently several were unable to read the cryostat technique pdf file that } Dr. Greg Erdos had generously posted at his website. The answer to the } problem could be the version of Acrobat used. I had the "blank page" } problem with version 3.0 but no problem at all with version 4.0 (Mac).
We have an ISI SS40 SEM that is in need of a discontinued part. It is a NEC transistor, number D588. It is used in the filament current control circuit. I'm having trouble locating the part because it has not been manufactured by NEC since 1984. Does anyone know of a source for this part or a substitute transistor?
Thanks in advance,
Bill Carmichael
______________________________________ Bill Carmichael Electron Microscopy Faculty Madison Area Technical College 3550 Anderson St. Madison, WI 53704 608-243-4309 wcarmichael-at-madison.tec.wi.us
Dominique, Glass is readily microtomed with a diamond knife and may be a suitable inexpensive technique for you to consider. Particularly if the materials of your sample are sufficiently dissimilar in reaction to the chemical and ion etching of some techniques. I've been embedding and sectioning coated glass, optics, and other hard materials for 18 years (even diamond coated silicon in Microscopy Research and Technique, vol. 31, p. 308, 1995). The imaging and analysis of nano-structures in micron sized areas near the surface of glass is routine, fast, and inexpensive for physical microstructure and chemistry. Mechanical artifacts generated in ultramicrotomy tend to be quite large, readily visible, and easily ignored but may interfere with the analysis of naturally occurring deformation features (i.e. twinning, slip, etc.). Any good diamond knife will work with meticulous and careful technique, but experience has shown that 35 degree knives yield the best results with hard and ultra-hard materials.
The critical elements for microtomy of hard, non-porous materials include: 1. Minimize the cross-sectional area to be sectioned. An easy way is to do this is to pop concoidal micro-chips from the surface. These tend to be very thin at the edges and may be further broken to form very pointed thin samples. [Time = ~20 minutes] 2. Optimize sample orientation for sectioning and preferred orientation. Some physical microstructures are anisotropic and are difficult to interpret when viewed in the wrong orientation. [Time = ~10 minutes] 3. Maximize adhesion to the resin through the selection of an appropriate resin (low viscosity and non-reactive with your sample), meticulous and contamination-free sample prep, and the addition of adhesion promoters (such as Dow Corning Z-6040). [Time = ~1 hour, with an over night epoxy cure] 4. Section using standard procedures, but minimize the sectioning speed (optimize cutting speed). [Time = ~1 hour]
These times are approximate for 1 sample, and there could be economy in numbers. As always, each case will require individual attention. Cheers,
Phil Swab Engineering Development Deposition Sciences Inc. Santa Rosa, CA 707-566-3718 phil.swab-at-depsci.com
-----Original Message----- } From: Schryvers Dominique [SMTP:schryver-at-ruca.ua.ac.be] Sent: Tuesday, January 25, 2000 6:51 AM To: Microscopy MAIL
Dear all,
we're starting a project of HRTEM on small (nanoscale) colouring metal particles in glass. Does anyone have experience with sample preparation for this type of material. We prefer ion-milling as this retains the relative positions of the particles with respect to the surface. Any suggestions, also on literature, are welcome.
Many thanks in advance,
Nick Schryvers
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=* *=* *=* *=* Dr. D. Schryvers *=* *=* Electron Microscopy for Materials Research (EMAT) *=* *=* University of Antwerp, RUCA *=* *=* Groenenborgerlaan 171 *=* *=* B-2020 ANTWERP *=* *=* Belgium *=* *=* tel: 32-3-2180247 *=* *=* fax: 32-3-2180257 *=* *=* e-mail: schryver-at-ruca.ua.ac.be *=* *=* homepage: http://www.ruca.ua.ac.be/EMAT *=* *=* *=* *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
we are analyzing frequently this kind of specimens. In our case the particles are silver. Depending on density and size distribution they are changing the color of the glass. Since we are interested in the depth distribution starting at the interface with a silver containging layer on the glass, we need to do a cross-sectional preparation. Therefore, we use several steps of preparation as described below:
á gluing of two coated glass surfaces face to face
á cutting and polishing of the obtained block to a size of 2 x 1 x 10 mmŌ with the interface plane running parallel to the 2 mm x 10 mm face and in the middle of the block
á gluing of this block in a steel-cylinder of 3 mm diameter and 10 mm length with a 1 mm x 2 mm rectangular hole along the cylinder axis
á cutting of 0.5 - 1 mm thick slices perpendicular to the cylinder axis
á fine polishing of one face of the obtained disk (lowest grain size: 1ĩm)
á grinding of the disk from the opposite face down to 100 ĩm thickness with subsequent fine polishing
á dimpling of a crater into one face of the disk with a residual thickness in the middle of the disk of about 10 ĩm
á ion etching of the flat side of the sample with argon ions of 4 kV under an angle of 2 - 4 degrees with a current of about 12 ĩA until electron transparency is reached in the region of the interface.
Some of the results were presented on the FEMMS-Meeting in Irsee, Germany, 1998. For more details, you might contact me directly.
Hope this helps,
Petra
At 15:50 25.01.00 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-------------------------------------------------------------- Dr. Petra Wahlbring Centre de Recherche Public - Gabriel Lippmann Laboratoire d'Analyse des Materiaux (LAM) 162a, av. de la Faiencerie L-1511 Luxembourg tel. +352-466644-402 fax +352-466644-400 e-mail: petra.wahlbring-at-crpgl.lu Visit our WWW site! http://www.crpgl.lu/~wahlbrin
Can anybody offer a method for the preparation of Halophilic bacteria for 'standard' SEM observation? post fixation washing appears to produce cell lysis!.
For those who are unable to view the PDF file of Cryostat information that I posted, I have also posted it in ugly HTML. I am still trying to find out why some can read the PDF and others cannot. The Version of Acrobat does not seem to be the answer.
My apologies to anyone who got frustrated. Once again the site is: www.biotech.ufl.edu/sems/
Greg Erdos Gregory W. Erdos, Ph.D. Ph. 352-392-1295 Assistant Director, Biotechnology Program PO Box 110580 Fax: 352-846-0251 University of Florida Gainesville, FL 32611
} } } {"wft03-at-health.state.ny.us"-at-sparc5.Microscopy.Com} 01/25 3:14 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } Aloha, } Tina } } It's raining cats and dogs, and it's cold and miserable. Feel better, } now? }
But what you call cold and what we call cold are two very different animals! Its been snowing all day in the Northeast, and the temps haven't nosed above (or even near) freezing) in days!
Dear Lee, Fair is fair. Often wind and rain at slightly above freezing is more miserable than snow at a few degrees below--having been both in San Francisco for the first condition and Albany NY for the second, I can say this with first-hand authority. Yours, Bill Tivol
} } } {"wft03-at-health.state.ny.us"-at-sparc5.Microscopy.Com} 01/25 3:14 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } Aloha, } Tina } } It's raining cats and dogs, and it's cold and miserable. Feel better, } now? }
But what you call cold and what we call cold are two very different animals! Its been snowing all day in the Northeast, and the temps haven't nosed above (or even near) freezing) in days!
Dear Lee, Fair is fair. Often wind and rain at slightly above freezing is more miserable than snow at a few degrees below--having been both in San Francisco for the first condition and Albany NY for the second, I can say this with first-hand authority. Yours, Bill Tivol
It would be convenient to be able to bring a video camera into an elementary school classroom, hook it up to a microscope (with an eyepiece adaptor) and display the microscope image for an entire classroom to see. If there is a video monitor (or a TV with a video input), this is fairly easy. If there is not an available monitor, I should be able to bring in a laptop and display the image on the laptop screen.
I am looking for an inexpensive lightweight video camera (with a C-mount) with either a firewire or USB linkage.
Does anyone have any suggestions?
Thanks
Peter Guthrie Department of Neurobiology & Anatomy University of Utah School of Medicine 50 N Medical Drive Salt Lake City, UT 84132 (801) 581-8336 (801) 581-4233 fax Peter.Guthrie-at-hsc.utah.edu
DearBill, When my Kevex detector showed degraded resolution, I turned off the bias and grounded the BNC plug with a paper clip, then warmed it up completely to get rid of ice and frost in the detector. This brought back my resolution, but degraded my LN2 holding time. Then I had a friend in Physics pump out the dewar and now I'm back to peak performance. 04:03 PM 1/25/00 -0500, you wrote: } Appropriate vendors, } I have a Kevex detector which has been giving peak widths } larger than specs. It probably just needs an overhaul, but there may } be some repair necessary for the pre-amp and FET. Could anyone } who can undertake this please respond to me off-list with estimates } for various contingencies? TIA. } Yours, } Bill Tivol Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Recently, an interesting medical school project has appeared at my lab door. I've been asked to quantify Ca and P in scar tissue found in sections of hamster hearts. Obtaining appropriate standards is critical. Are there commercially available biological standards or procedures to create such standards out there? Any suggestions would be appreciated and thanks for your time.
Dan
===================================================== Dan Kremser Washington University Department of Earth and Planetary Sciences/Campus Box 1169 (Wilson Hall, Room 108-----for packages) One Brookings Dr. St. Louis, MOĘ 63130-4899
How about using a device like Dazzle or Snappy to display the video stream from your video camera on a laptop? It would require a little software setup but should work. The models I am familiar with used parallel port connections, but there ought to be models around that would use USB.
At 09:28 AM 1/26/2000 -0700, you wrote: } It would be convenient to be able to bring a video camera into an } elementary school classroom, hook it up to a microscope (with an eyepiece } adaptor) and display the microscope image for an entire classroom to see. } If there is a video monitor (or a TV with a video input), this is fairly } easy. If there is not an available monitor, I should be able to bring in a } laptop and display the image on the laptop screen. } } I am looking for an inexpensive lightweight video camera (with a C-mount) } with either a firewire or USB linkage. } } Does anyone have any suggestions? } } Thanks
Listservers, Can anyone lead me to a good reference for processing human sperm for TEM? Or if you have a procedure can you please forward the details. TIA Hank Adams Integrated Microscopy Core Molecular and Cell Biology Baylor College of Medicine Houston, TX
Stefanini M, De Martino C, Zamboni L. 1967. Fixation of ejaculated spermatozoa for electron microscopy. Nature 216:173-174.
Meschede D, Keck C, Zander M, Cooper TG, Yeung CH, Nieschlag E. 1993. Influence of three different preparation techniques on the results of human sperm morphology analysis. Int J Androl 16:362-369.
Phillips DM. 1995. Fixation of mammalian spermatozoa for electron microscopy. In: Dentler W, Witman G (Eds.), Methods in Cell Biology, Vol 47, vol 47. Academic Press Inc (San Diego), pp 199-204.
Kent
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A. Kent Christensen, Professor Emeritus Department of Cell and Developmental Biology, Medical Science II Building University of Michigan Medical School, Ann Arbor, MI 48109-0616 Office: 2703A Medical Science II Building Tel (work) (734) 763-1287, Fax (work) (734) 763-1166 E-mail: akc-at-umich.edu http://www.umich.edu/~akc/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Listservers, } Can anyone lead me to a good reference for processing human sperm for } TEM? Or if you have a procedure can you please forward the details. TIA } Hank Adams } Integrated Microscopy Core } Molecular and Cell Biology } Baylor College of Medicine } Houston, TX
If you intend to use a "video" camera, you may need a capture card rather than, or in addition to, a USB or Firewire interface. Most video cams are analog (NTSC in the US, SECAM or PAL elsewhere in the world). Others can certainly have digital circuits internal (like the new digital camcorders or the PCcams used for video conferencing on the net). ...You first need to determine the source format.
Warren's suggestion implements an inexpensive external NTSC* video frame grabber (Snappy). Which will "grab" an analog video frame and digitize it. *May do PAL too??
Parallel port I/O is a bit slow (or is that a byte slow :) for pictures containing lots of data, but is cheap and works since the inherent resolution of typical NTSC video is { 640x480. USB is very much faster and Firewire faster yet (and usually a lot more $$ for the interface card). For applications other than full frame rate/resolution streaming video, USB is fine.
They have a USB version and the software interface is great. It is easy to use and has a street price of about $180!
Good Luck,
Lawrence Kordon Nikon, Inc. Columbia, Maryland nikon-at-jagunet.com
***I have no affiliation with Dazzle, Inc.***
"White, Woody N" wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Peter, } } If you intend to use a "video" camera, you may need a capture card rather } than, } or in addition to, a USB or Firewire interface. Most video cams are analog } (NTSC in the US, SECAM or PAL elsewhere in the world). Others can certainly } have digital circuits internal (like the new digital camcorders or the } PCcams } used for video conferencing on the net). ...You first need to determine } the } source format. } } Warren's suggestion implements an inexpensive external NTSC* video frame } grabber } (Snappy). Which will "grab" an analog video frame and digitize it. *May do } PAL } too?? } } Parallel port I/O is a bit slow (or is that a byte slow :) for pictures } containing lots of data, but is cheap and works since the inherent } resolution of } typical NTSC video is { 640x480. USB is very much faster and Firewire } faster } yet (and usually a lot more $$ for the interface card). For applications } other } than full frame rate/resolution streaming video, USB is fine. } } Woody White
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} Please keep weather reports private. } } Ann Fook
Why? A little light heartedness never hurt anyone. For the record, LA was sunny as usual today. Happy I don't live in CT anymore.
Regards,
Paul Webster, Ph.D. Associate Scientist & Director Ahmanson Advanced Electron Microscopy & Imaging Center House Ear Institute 2100 West Third St. Los Angeles, CA 90057
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id UAA11239 for dist-Microscopy; Wed, 26 Jan 2000 20:43:46 -0600 (CST) Received: from no_more_spam.com (sparc5.microscopy.com [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id UAA11236 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Wed, 26 Jan 2000 20:43:16 -0600 (CST) Received: from staff2.cso.uiuc.edu (staff2.cso.uiuc.edu [128.174.5.53]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id UAA11229 for {Microscopy-at-sparc5.Microscopy.Com} ; Wed, 26 Jan 2000 20:43:05 -0600 (CST) Received: from [130.126.25.46] (rochester-46.slip.uiuc.edu [130.126.25.46]) by staff2.cso.uiuc.edu (8.9.3/8.9.3) with ESMTP id UAA27358 for {Microscopy-at-sparc5.Microscopy.Com} ; Wed, 26 Jan 2000 20:38:21 -0600 (CST) Mime-Version: 1.0 X-Sender: lamiller-at-ux1.cso.uiuc.edu Message-Id: {v04210100b4b55eb12196-at-[130.126.26.199]}
I currently video with a Sony DV Video camera, fire wire connect to my computer with no capture board.
It does require special software however, We bought Final Cut Pro, But from trying out the demo and reading, It appears Adobe Priemier also will allow firewire capture without a board. Though Adobe's is one I have not tried, I'd call first.
This works fine for video, and I can pull off individual frames for low res images for the web, and ok small images to print if very small.
But, in emailing to and from Sony, It was my understanding that if I were to firewire images, I WOULD need a board.
My video camera will do both images and video. FinalCut Pro pulls off the video, but I can't seem to get it to recognize the individual image shots. So I believe Sony, though I may just not have selected the right buttons etc.
If pulling in video by fire wire, especially pulling it onto a firewire hard drive ( ie VST) It is pretty close to real time video.
Lou Ann *************************** Lou Ann Miller Service Supervisor Microscopic Imaging Lab College of Vet. Medicine University of Illinois 2001 S Lincoln Ave Urbana,Illinois 61801 217-244-1566 lamiller-at-ux1.cso.uiuc.edu
Microscopy Home Page: http://treefrog.cvm.uiuc.edu
Central States Microscopy Society http://treefrog.cvm.uiuc.edu/csmms
Personal Home Page: http://treefrog.cvm.uiuc.edu/lam
I did some TEM work on the extreme holophile bacterias (they grow in saturated NaCl solutions) some 30 years ago. Very difficult specimens, it seems no fixation is complete and can prevent osmotic shock.
I have not tried SEM on halophiles, but I suggest this: You could try excessive fixation, using 2 hours at room 20 degrees. I would use a several molar solution of ammonium acetate to rinse the specimen after fixation. Ammonium acetate is a volatile salt solution and leaves no crystals after evaporation. The still wet sample (mounted on a 10mm coverslip) could then be placed in a glass Petrie dish which has a double layer of filter paper, saturated with chloroform. Place the closed Petrie dish in the fridge for a day or two. Warm the dish before opening (to avoid condensation) and metal coat prior to SEM.
Ah, your first problem could be the fixation. The osmium (I'd forget GA), would need to go into the bacteria's growth medium, or use vapour fixation only. Even then, I expect much damage before any further processing. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Thursday, January 27, 2000 12:01 AM, Hyman, S.C. [SMTP:sch10-at-leicester.ac.uk] wrote: } } } Can anybody offer a method for the preparation of Halophilic bacteria for } 'standard' SEM observation? post fixation washing appears to produce cell } lysis!.
Moreover, it is well known that weather may affect the quality of EM samples. For instance, humidity is very critical for many EM techniques. I utilized very unusual technique for holey-film preparation with calcium-rhodanide. This technique is extremely sensitive for humidity/temperature combination. When I was working in Russia (without conditioner in the room), I was able predict the weather changes using that technique. Again, it was tricky to work when temperature in the room was around 7oC (at winter). The guys from East Coast may have something like that. Why not to share experience how to work at different weather conditions?
Have a good weather!
Sergey
} Date: Wed, 26 Jan 2000 17:52:26 -0800 } From: Paul Webster {pwebster-at-hei.org} } Subject: Re:No weather report please } To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com} } Reply-to: Paul Webster {pwebster-at-hei.org} } X-Mailer: QuickMail Pro 1.5.4 (Mac) } X-MIME-Autoconverted: from quoted-printable to 8bit by ultra5.microscopy.com id } TAA11085 } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Dear Hank and others, I have had good success this Stefanini's buffered picric acid paraformaldehyde (PAF) for spermatozoan. I do not have the fixative formula at my fingertips, but the reference is: Nature Vol. 216, OCT 14. 1967.
I will look it up if you are interested and get back to you or you can email me-at- tiekotte-at-up.edu. -Ken
Ken Tiekotter Dept. of Biology The University of Portland 5000 N Willamette Blvd. Portland, OR 97303
On Wed, 26 Jan 2000, hank adams wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Listservers, } Can anyone lead me to a good reference for processing human sperm for TEM? Or if you have a procedure can you please forward the details. } TIA } Hank Adams } Integrated Microscopy Core } Molecular and Cell Biology } Baylor College of Medicine } Houston, TX } }
} From: Greg Erdos {gwe-at-biotech.ufl.edu} } To: Microscopy-at-sparc5.microscopy.com } Subject: Cryostat info. } Date: Wed, 26 Jan 2000 09:19:58 -0500 } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } For those who are unable to view the PDF file of Cryostat information that } I posted, I have also posted it in ugly HTML. I am still trying to find } out why some can read the PDF and others cannot. The Version of Acrobat } does not seem to be the answer. } } My apologies to anyone who got frustrated. } Once again the site is: } www.biotech.ufl.edu/sems/ } } Greg Erdos } Gregory W. Erdos, Ph.D. Ph. } 352-392-1295 } Assistant Director, Biotechnology Program } PO Box 110580 Fax: } 352-846-0251 } University of Florida } Gainesville, FL 32611 }
I think that we are proceeding slightly tongue-in-cheek here - but to continue the 'serious' note;
The most weather sensitive task I have ever tackled is the production of formvar films. With the extremely high humidities we experience here there are times when the formvar will simply NOT separate from the substrate, despite 'air-conditioning'.
Tony
Tony Bruton Head, Centre for Electron Microscopy University of Natal, Pietermaritzburg Tel +27 (0) 33 260 5155 Fax +27 (0) 33 260 5776 website via:http:www.nu.ac.za Email:bruton-at-emu.unp.ac.za postal address; Private Bag X01, Scottsville, 3209 KwaZulu-Natal South Africa
} } } Sergey Ryazantsev {sryazant-at-ucla.edu} 01/27/00 10:31AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Moreover, it is well known that weather may affect the quality of EM samples. For instance, humidity is very critical for many EM techniques. I utilized very unusual technique for holey-film preparation with calcium-rhodanide. This technique is extremely sensitive for humidity/temperature combination. When I was working in Russia (without conditioner in the room), I was able predict the weather changes using that technique. Again, it was tricky to work when temperature in the room was around 7oC (at winter). The guys from East Coast may have something like that. Why not to share experience how to work at different weather conditions?
Have a good weather!
Sergey
} Date: Wed, 26 Jan 2000 17:52:26 -0800 } From: Paul Webster {pwebster-at-hei.org} } Subject: Re:No weather report please } To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com} } Reply-to: Paul Webster {pwebster-at-hei.org} } X-Mailer: QuickMail Pro 1.5.4 (Mac) } X-MIME-Autoconverted: from quoted-printable to 8bit by ultra5.microscopy.com id } TAA11085 } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Can anyone tell me who currently makes and sells the retrograde neuronal tracer, Fluorogold? We have a customer who is confusing it with our FluoroNanogold products (not the first time this has happenned) and I would lkke to point them to the right source!
Thanks,
Rick Powell
********************************************************************** * NANOPROBES, Incorporated | US Toll-free: (877) 808-2101 * * 25 East Loop Road, Suite 113 | Tel: (919) 510-0590 * * Stony Brook, NY 11790-3350, | Fax: (919) 510-0590 * * USA | rpowell-at-lihti.org * * * * NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com * **********************************************************************
The main reference is: Stefanini et al., 1967, Nature 216:173.
Ramin Rahbari PARKE-DAVIS Pharmaceutical Research Worldwide Preclinical Safety 2800 Plymouth Road Ann Arbor, MI 48105 Voice (734) 622-3383 Fax (734) 622-5001 Ramin.Rahbari-at-WL.COM
-----Original Message----- } From: hank adams [mailto:hpadams-at-bcm.tmc.edu] Sent: Wednesday, January 26, 2000 2:39 PM To: 'microscopy-at-msa.microscopy.com'
Listservers, Can anyone lead me to a good reference for processing human sperm for TEM? Or if you have a procedure can you please forward the details. TIA Hank Adams Integrated Microscopy Core Molecular and Cell Biology Baylor College of Medicine Houston, TX
If anyone remembers I use to end all my e-mails to the listees with a quite sarcastic weather and/or olfactory report from the garden state. Either no one read my posts or they just didn't get the East Coast thing.
Cold, windy and something smells real odd under the Pulaski Skyway...Aloha!
John Grazul Lucent
Sergey Ryazantsev wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Moreover, it is well known that weather may affect the quality of EM } samples. For instance, humidity is very critical for many EM techniques. I } utilized very unusual technique for holey-film preparation with } calcium-rhodanide. This technique is extremely sensitive for } humidity/temperature combination. When I was working in Russia (without } conditioner in the room), I was able predict the weather changes using that } technique. Again, it was tricky to work when temperature in the room was } around 7oC (at winter). The guys from East Coast may have something like } that. Why not to share experience how to work at different weather } conditions? } } Have a good weather! } } Sergey } } } Date: Wed, 26 Jan 2000 17:52:26 -0800 } } From: Paul Webster {pwebster-at-hei.org} } } Subject: Re:No weather report please } } To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com} } } Reply-to: Paul Webster {pwebster-at-hei.org} } } X-Mailer: QuickMail Pro 1.5.4 (Mac) } } X-MIME-Autoconverted: from quoted-printable to 8bit by } ultra5.microscopy.com id } } TAA11085 } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } } Please keep weather reports private. } } } } } } Ann Fook } } } } Why? A little light heartedness never hurt anyone. } } For the record, LA was sunny as usual today. Happy I don't live in CT } anymore. } } } } Regards, } } } } Paul Webster, Ph.D. } } Associate Scientist & Director } } Ahmanson Advanced Electron Microscopy & Imaging Center } } House Ear Institute } } 2100 West Third St. } } Los Angeles, CA 90057 } } } } Phone: (213) 273-8026 } } Fax: (213) 413-6739 } } e-mail: pwebster-at-hei.org } } http://www.hei.org/htm/aemi.htm } } } } } } } _____________________________________ } } Sergey Ryazantsev Ph. D. } Electron Microscopy } UCLA School of Medicine } Department of Biological Chemistry } Box 951737 } Los Angeles, CA 90095-1737 } } Phone: (310) 825-1144 } Pager: (310) 845-0248 } FAX (departmental): (310) 206-5272 } mailto:sryazant-at-ucla.edu } http://www.bol.ucla.edu/~sryazant
Molecular Probes sells hydroxystilbamidine, methanesulfonate (cat. # H-7599) which I beleive is the active chemical in Fluorogold. (see paper by Martin W. Wessendorf in Brain Res 553(1): 135-48. Jul 1991).
Karen Zaruba
P.S. I have no interest in Molecular Probes other than a satisfied customer.
Rick Powell at Nanoprobes wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hello Light Microscopists: } } Can anyone tell me who currently makes and sells the retrograde neuronal } tracer, Fluorogold? We have a customer who is confusing it with our } FluoroNanogold products (not the first time this has happenned) and I would } lkke to point them to the right source! } } Thanks, } } Rick Powell } } ********************************************************************** } * NANOPROBES, Incorporated | US Toll-free: (877) 808-2101 * } * 25 East Loop Road, Suite 113 | Tel: (919) 510-0590 * } * Stony Brook, NY 11790-3350, | Fax: (919) 510-0590 * } * USA | rpowell-at-lihti.org * } * * } * NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com * } **********************************************************************
-- Karen S. Zaruba kszaruba-at-mmm.com 3M Company, St. Paul, MN
Tried to respond to a message posted here from Cynthia Shannon re: a used TEM, it keeps bouncing. Cynthia, if you are out there, how can I contact you?
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
ELECTRON MICROSCOPY TECHNICIAN The Integrated Microscopy Core, Department of Molecular and Cell Biology, Baylor College of Medicine is expanding and has an immediate full-time opening for an electron microscopy technician. The Integrated Microscopy Core is a growing, state-of-the-art facility with 2 TEMs, deconvolution, laser scanning confocal, 2 CCD-based fluorescent microscopes and multiple PC and Silicon Graphics workstations for imaging software. The applicant should have at least one year of experience in various aspects of sample preparation for biological TEM including fixation, embedding, ultrathin sectioning and staining. The applicant should have darkroom experience and experience in the operation of TEMs. Other duties include preparation of solutions, embedding media and the maintaining of records. The position offers excellent opportunities for training in advanced light and electron microscopy techniques, including immunofluorescence and immunogold labeling, laser scanning confocal and deconvolution microscopy, as well as live imaging of GFP-tagged proteins. Training in several image computer-based imaging programs will also be available (PhotoShop, MetaMorph, SoftWorks). The position requires a minimum of a Bachelors degree and will start as a Lab Technician II; salary will be commensurate with experience, and includes the standard Baylor benefits package.
Send CV and letter of research/technical interests to:
Hank Adams Laboratory Manager Integrated Microscopy Core Department of Molecular and Cell Biology Baylor College of Medicine One Baylor Plaza Houston, TX 77030 Email submissions to: hpadams-at-bcm.tmc.edu Fax submissions to: 713 790 0545
Baylor College of Medicine is an Equal Opportunity, Affirmative Action and Equal Access Employer.
I can't see how it can be netscape vs IE, since I got the blank page using IE4. I haven't tried netscape or IE5 (have both at home--but not here at work). Acrobat seems to work on every other PDF file I have opened (the intranet and internet standard here for public documents in a read-only setting), so I don't think the version of Acrobat is the problem either. I tried Dr. Erdos' original work-around, but still got the blank page. ????
On Thu, 27 Jan 2000 11:36:12 +0100, Shu-You Li wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Dr. Erdos, } } Perheps it is due to the difference between Netscape and IE. I got a blank } page with netscape but read it correctly with IE4.0. } } Shu-You Li } ************************************************** } Shu-You Li, Dr. } Institut fuer Physikalische Chemie } Johannes Guttenberg Universitaet } Jakob-Welder-Weg 11 } D-55099 Mainz, Germany } } E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com } Fax: +49-6131-3923768 } Tel: +49-6131-3923148(O) } ************************************************** } } } } } From: Greg Erdos {gwe-at-biotech.ufl.edu} } } To: Microscopy-at-sparc5.microscopy.com } } Subject: Cryostat info. } } Date: Wed, 26 Jan 2000 09:19:58 -0500 } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } For those who are unable to view the PDF file of Cryostat information that } } I posted, I have also posted it in ugly HTML. I am still trying to find } } out why some can read the PDF and others cannot. The Version of Acrobat } } does not seem to be the answer. } } } } My apologies to anyone who got frustrated. } } Once again the site is: } } www.biotech.ufl.edu/sems/ } } } } Greg Erdos } } Gregory W. Erdos, Ph.D. Ph. } } 352-392-1295 } } Assistant Director, Biotechnology Program } } PO Box 110580 Fax: } } 352-846-0251 } } University of Florida } } Gainesville, FL 32611 } } }
Roger C Moretz, Ph.D.
_______________________________________________________ Get 100% FREE Internet Access powered by Excite Visit http://freeworld.excite.com
I think, there is some confusion out there about digital and analog cameras and different protocols and interfaces. Woody's posting is correct, but perhaps a look at some of the current implementations might help:
1) Cameras come either with a digital output or analog output. As Woody mentions, there are several different analog standards (PAL, NTSC, RS-170,...) and formats for transmitting the data (RGB, S-VHS, composite, ...)
2) Regardless of what the signal is, there must be some "device" that translates the incoming signals into "numbers" that the computer can understand. Sometimes this is implemented on the motherboards (USB), or needs an additional card (frame grabber). It depends on the age of the computer and its make and Operating system which of the different options are supported.
3) Currently there are 3 ways of getting the signals into a PC (other than serial RS-232 and parallel ports which are way to slow):
a) USB b) 1394 or firewire c) PCI boards
USB
USB is a serial interface that is now supported on most computers out of the box. The bandwidth of a USB connection is a maximum of 12 MegaBIT/second, which translates of course into 1.5 Mega-BYTE per second. This is too slow for video (about 4-5 MegaByte per second), but enough for still images, unless the video is compressed. Compression is OK for "consumer" video, but not acceptable for "scientific" video unless it is lossless (JPEG and MPEG is usually not). Several devices on the USB bus can compete for bandwidth.
1394
1394 (or firewire) is also a serial interface with a maximum bandwidth of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast enough for video. However, I believe that again more than one device can be attached to a 1394 port which will again compete for bandwidth. Firewire is not generally available on PCs (I believe the newer Macs have it), so that for a firewire implementation on a PC normally a card is necessary that fits into a PCI slot.
PCI
PCI boards, while a bit more cumbersome to install, have the highest throughput. I think, they are now implementing a new PCI-X specification that allows up to 1 GigaBYTE per second, i.e., about 20 times faster than firewire. The reason is of course that PCI is a parallel standard and not a serial like USB or firewire.
So, there are various ways to attach a camera:
Analog camera: There is no other way than to use a board to transform the analog signals into digital signals and then send them to the computer. This can be through a PCI or other card or other electronics for example on the video card, but the translation is necessary.
Digital cameras: Digital cameras essentially put the digitizer into the camera and then transmit digital signals. They can then be transferred through USB (slow but available everywhere), firewire (faster, currently on Macs (I believe) and PCs with additional card), or through boards for the PCI bus (fastest, widely available, require board installation).
So, for most users (of PCs at least) there is currently no real difference between using a firewire or other camera, as they either have to install a PCI-} firewire card, or another PCI card for image acquisition. That may change if the motherboard manufacturers start building firewire circuitry into the motherboards and the operating systems start supporting this option. For highest performance, however, I believe that we will see PCI boards for some time to come. Firewire may run into a performance problem in the future for image streams with large images. A 1600x1200 image stream with 24 bit color and 30 frames per second requires a bandwidth of about 170 MBytes/second uncompressed.
I hope I have not confused anybody with this.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
} ---------- } From: White, Woody N[SMTP:WOODY.N.WHITE-at-MCDERMOTT.COM] } Sent: Wednesday, January 26, 2000 4:13:00 PM } To: "Peter Guthrie" ; Microscopy-at-sparc5.Microscopy.Com } Subject: Re:USB or firewire cameras } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Peter,
If you intend to use a "video" camera, you may need a capture card rather than, or in addition to, a USB or Firewire interface. Most video cams are analog (NTSC in the US, SECAM or PAL elsewhere in the world). Others can certainly have digital circuits internal (like the new digital camcorders or the PCcams used for video conferencing on the net). ...You first need to determine the source format.
Warren's suggestion implements an inexpensive external NTSC* video frame grabber (Snappy). Which will "grab" an analog video frame and digitize it. *May do PAL too??
Parallel port I/O is a bit slow (or is that a byte slow :) for pictures containing lots of data, but is cheap and works since the inherent resolution of typical NTSC video is { 640x480. USB is very much faster and Firewire faster yet (and usually a lot more $$ for the interface card). For applications other than full frame rate/resolution streaming video, USB is fine.
Woody White
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
The IMAC DV comes with a firewire port standard and a firewire cable. Connection to a Sony DV camcorder is easy and it gives you complete control from the computer. See http://www.apple.com/firewire/ for more information.
To get firewire into a PCI (mac or windows) machine Sony sells this card http://www.sel.sony.com/SEL/consumer/ss5/office/digitalvideo/minidvcamcorderspro ducts/dvbk-2000_specs.shtml for around $350 which gives similar controls for live video and digital stills.
No interest in either company except as a satisfied customer. Scott
} } 1394 (or firewire) is also a serial interface with a maximum bandwidth } of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast } enough for video. However, I believe that again more than one device can } be attached to a 1394 port which will again compete for bandwidth. } Firewire is not generally available on PCs (I believe the newer Macs } have it), so that for a firewire implementation on a PC normally a card } is necessary that fits into a PCI slot. } ..snip... } So, for most users (of PCs at least) there is currently no real } difference between using a firewire or other camera, as they either have } to install a PCI-} firewire card, or another PCI card for image } acquisition. That may change if the motherboard manufacturers start } building firewire circuitry into the motherboards and the operating } systems start supporting this option. For highest performance, however, } I believe that we will see PCI boards for some time to come. Firewire } may run into a performance problem in the future for image streams with } large images. A 1600x1200 image stream with 24 bit color and 30 frames } per second requires a bandwidth of about 170 MBytes/second uncompressed. } } Michael Bode, Ph.D.
-------------------note: new mailing address---------------------- Scott Wight e-mail: scott.wight-at-nist.gov NIST 222/A113 W voice: 301-975-3949 100 Bureau Dr STOP 8371 | fax: 301-417-1321 Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed is my own and does not represent those of my employer.
-----Original Message----- } From: Paul Webster [mailto:pwebster-at-hei.org] Sent: Wednesday, January 26, 2000 6:52 PM To: MSA listserver submission
} Please keep weather reports private. } } Ann Fook
Why? A little light heartedness never hurt anyone. For the record, LA was sunny as usual today. Happy I don't live in CT anymore.
Regards,
Paul Webster, Ph.D. Associate Scientist & Director Ahmanson Advanced Electron Microscopy & Imaging Center House Ear Institute 2100 West Third St. Los Angeles, CA 90057
We would apreciate to inform interested young researcher of the following available positions in our research project. Thank you very much for getting this circulated. Pierre
Pierre Ruterana Laboratoire d'Etude et de Recherche sur les Materiaux (LERMAT) Unite associee CNRS No 6004 Institut Superieur de la Matiere et du Rayonnement(ISMRA) 6, Bd Marechal Juin 14050 Caen Cedex France Tel: (33 2) 31 45 26 53 Fax:(33 2) 31 45 26 60 e-mail: ruterana-at-lermat8.ismra.fr
Research Training Network EC Contract NĄ: HPRN-CT-1999-00040 Interface analysis at atomic level and Properties of Advanced Materials (IPAM)
Eight positions are immediately available, eligible young ( { 35 years) researchers must be citizens of EC or associated countries (Norway, Island, Israel, Lichtenstein, Bulgaria, the Czech Republic, Estonia, Hungary, Lithuania, Poland, Romania, Slovakia, Slovenia and Letonia), however any foreigner who has spent five years in an EC country may apply. Women candidates are particularly encouraged to apply and equal opportunity between women and men will govern our choice. Following the mobility criteria, the young researchers will not apply for a position in their native country.
1. POSTDOCTORAL POSITION at Fritz Haber Institute, Max Planck Society, Berlin, Germany A postdoctoral position at the Fritz-Haber-Institut in Berlin (Germany) is available in the group "Surface morphology and growth of semiconductors" under the supervision of Dr. Joerg Neugebauer. The research will be mainly focused on the theoretical modeling of electronic properties and atomic structure of interfaces and interfacial defects employing first principles total energy calculations. The basic materials for this project will be gallium nitride based semiconducting layers where extended defects are well known to occur in large concentrations. The research will be performed in close collaboration with experimental, industrial, and theoretical partners within the EC. Strong interaction with the other groups is therefore expected. The successful candidate should have a PhD in Physics, Chemistry or Materials Science, and have a strong interest on microscopic simulations. Preference will be given to candidates with strong background in any (or several) of these fields: electronic structure calculations, molecular modeling, density functional theory, empirical potentials, and analysis of transmission electron microscopy measurements. Interested candidates should send immediately a CV, list of publications, name and address (including email) of three references, preferably by email or fax, to: Dr Joerg Neugebauer E-mail: neugebauer-at-fhi-berlin.mpg.de, Phone: ++49 30 8413 4826, Fax: ++49 30 8413 4701, www: http://www.fhi-berlin.mpg.de/th/JG
2. POSTDOCTORAL POSITION at Universitat Politcnica de Catalunya, Barcelona, Spain A postdoctoral position is available at the department of Applied Mathematics in the UPC. We are seeking a computational materials scientist interested in modeling the atomic structure of interfaces and defects in crystals, mainly gallium nitride based materials. A PhD in Physics, Materials Science or related discipline and having experience with atomic simulations is required. It is highly desirable an ability to develop empirical interatomic potentials. It is intended that he/she visits the other laboratories working in the project to learn how to interpret the experimental observations and how to use the theoretical concepts. Interested candidates should send immediately a CV, list of publications, name and address (including email) of three references, preferably by email or fax, to: Prof. Anna Serra E-Mail: a.serra-at-upc.es, Phone: ++34 93 401 68 86, Fax: ++ 34 93 401 18 25
3. A FULL TIME PhD STUDENT at CRHEA, Valbonne, France These last years, CRHEA-CNRS has implemented an expertise in the growth of heteroepitaxial GaN layers on different substrates: sapphire, SiC and Si by different techniques, MBE, MOVPE and HVPE. The group has developed a proprietary Epitaxial Lateral Overgrowth (ELO) technology which allows to decrease by orders of magnitude the density of dislocations in GaN heteroepitaxial layers on sapphire, SiC or Si. Therefore, a great interest in the procurement of high quality GaN substrates currently exists. The successful candidate will strongly support our present effort to produce self-supported GaN of ELO quality by combining ELO-MOVPE and HVPE. Parallel to the growth, he will contribute to the development of in depth analysis of basis mechanisms linked to the generation and propagation of threading dislocations(TDs). More precisely, it is planned to determine the core structure of defects in ELO GaN, their electronic structures (by EELS), the mechanism of bending of these TDs, to implement new ways of further decreasing the density of dislocations. He will be able to use two MOVPE, one HVPE reactor and all basic characterisation tools (double X-ray diffraction, magnetotransport, low temperature photoluminescence, HRTEM,.). Candidates should send immediately a CV, name and address (including email) of two references, preferably by email or fax, to: Dr Pierre Gibart, email: Pierre.Gibart-at-crhea.cnrs.fr, Tel: ++33 4 93 95 42 27, Fax: ++33 4 93 95 83 61 CHREA-CNRS is located at the French Riviera near Nice ( see: http://www.crhea.cnrs.fr/)
4. POSTDOCTORAL POSITION at ISMRA Caen, France Candidates should preferably have a Phd with experience in electron microscopy and/or atomic structure modeling. The project will involve experimental high resolution electron microscopy and image analysis. In parallel, atomic structure modeling of defects and interfaces will use empirical and tight binding methods. A connection will be established with ab initio techniques developed in partner groups and the successful candidate will undertake quantitative comparison of experimental and simulated images. The overall aim is the understanding of the role of defects and interfaces on the optoelectronic properties in the Ga based nitride semiconductors. Candidates should send immediately a CV, list of publications, name and address (including email) of three references, preferably by email or fax, to: Dr Gerard Nouet on ++33 2 31 45 26 47, email:gerard.nouet-at-labolermat.ismra.fr or Dr Pierre Ruterana on ++33 2 31 45 26 53 email: ruterana-at-lermat8.ismra.fr
5. POSTDOCTORAL POSITION at University of Liverpool, Great Britain A three year full-time appointment funded by the European Commission is available to study defect mechanisms in gallium nitride based electronic device structures within the III-V semiconductor materials group. Candidates should preferably have postgraduate experience in the growth or processing of semiconductor device materials. The project will involve the chemical beam epitaxy of GaN based materials and the fabrication of model device structures. The influence of processing parameters on defect propagation will be investigated using analytical methods such as electron microscopy, Raman spectroscopy and surface analytical techniques. This appointment is part of a Research Training Network and eligible candidates must be citizens of EC member countries other than the United Kingdom. Candidates should send immediately a CV, list of publications, name and address (including email) of three references, preferably by email or fax, to: Dr Paul Chalker: ++44 151 794 4313, email pchalker-at-liv.ac.uk or Prof Robert Pond: ++44 151 794 43 13 / 46 60, email R.C.Pond-at-liverpool.ac.uk
6. POSTDOCTORAL POSITION at Universitt Erlangen-Nrnberg, Germany Focus of the work in Erlangen university will be on direct correlation of structural, optical and electrical properties of extended defects in (i) group-III nitrides (ii) group III-nitride based heterostructures. Experimental work is based on (scanning) transmission electron microscopy ((S)TEM) at all levels of resolution. These comprise high resolution imaging with atomic resolution, optical characterisation by cathodoluminescence in the STEM and analysis of electrical properties (electrical activity of extended defects, diffusion length of minority carriers) by the electron beam induced current (EBIC) both in the SEM and the STEM. Theoretical analyses are based on TEM contrast simulation for defect analysis, analysis of the tetragonal distortion from high resolution TEM images and finite element simulations of the strained state of heterostructures. Candidates should send immediately a CV, list of publications, name and address (including email) of three references, preferably by email or fax, to: Prof Horst Strunk, Tel: ++49 9131 85 2 8601, Fax: ++49 9131 85 2 8602, email: strunk-at-cmp03ww7.ww.uni-erlangen.de, Dr Martin Albrecht, Tel.: ++49 9131 85 2 8613, Fax: ++49 9131 85 2 8602, e-mail: albrecht-at-cmp04ww7.ww.uni-erlangen.de,
7. POSTDOCTORAL POSITION at the Aristotle University of Thessaloniki, Greece A research opportunity is available for postdoctoral candidates with a background in Electron Microscopy, Crystal Growth, Materials Science. The primary responsibility of the candidate will be the study of the structure and properties of the heterophase interfaces between thin films on gallium nitride based materials. The project will offer the necessary training for the specific skills to meet the requirements of the job. Candidates should send immediately a CV, list of publications, and name and address (including email) of three references, preferably by email or fax, to: Prof Philomela Komninou, Tel: ++30 31 99 81 95 / Fax: ++30 31 99 80 61 e-mail: komnhnoy-at-auth.gr
8. A FULL TIME PhD STUDENT at The University of Cambridge, Great Britain A three year research studentship position leading to a PhD degree is available to study the microscopy and analysis of defects in gallium nitride layers and device structures. This exciting project will use a wide range of state-of-the-art electron microscopy and analysis techniques to study the atomic structure of defects (using high resolution electron microscopy), their chemical composition and electronic properties (using x-ray spectroscopy and electron energy loss spectroscopy), including which defects give rise to states in the band gap. This project is part of a European Research Training Network aimed at optimising devices in GaN-based materials. The research student will form strong links with the other European partners in this project. Eligible candidates should have a top quality degree in physics, chemistry, materials science or electrical engineering. Candidates should send immediately a CV, name and address (including email) of two references, preferably by email or fax, to: Prof Colin Humphreys on +44 1223 334457, email {colin.humphreys-at-msm.cam.ac.uk} , or Dr Dave Tricker on +44 1223 334469, email {dmt1000-at-cus.cam.ac.uk}
The Candidates will gain time by sending a copy of their CV also to Prof. Philomela Komninou, leader of the Training Programme. Tel: ++30 31 99 81 95 / Fax: ++30 31 99 80 61 e-mail: komnhnoy-at-auth.gr; Please indicate the position of interest.
Caen, January 27 2000 Dr. Pierre Ruterana Coordinator
It seems nobody replied to Michaels question. Perhaps because there is no single answer. LR White and LR Gold I would expect to have a very similar shelf-life - under similar conditions.
The trouble is to know the starting point. LR White slowly "goes off" from when the catalyst is added. At room temperature or higher this happens at a much faster rate than when it's kept refrigerated. Catalyzed LR White could be kept at the room temperatures for some months. Because the shipping time (even by air) to our home-market (Australia) from the UK is too long and often at high temperatures, much and an indeterminable part of the shelf-life would be expired prior to sale.
Consequently we only procure uncatalysed LR White and the end-user must add and thoroughly mix the catalyst prior to first use. Our users expect a full year of refrigerated shelf-life. Shelf-life is really a "when, how long and at what temperature" question. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Tuesday, January 25, 2000 4:52 PM, Michael Reiner [SMTP:michael.reiner-at-Smail.Uni-Koeln.de] wrote: } } } Dear members of the list, } } first, I would like to wish you all the best for the new year. } Now my question: } Does anyone know the shelf-life/usability of LR-Gold stored in a fridge? } } Is it that delicate as LR-White (Meanwhile I don?t use LR-W older than } half a year). My bottle which was not opened many times, could be } roundabout three years old. } } Thanks a lot, } Michael } } Michael Reiner } Department of Anatomy I } University of Cologne } Germany } michael.reiner-at-smail.uni-koeln.de
Dear list members, The intent of this note is to formally announce a call for papers for the upcoming Spring 2000 AReMS (Appalachian Regional Microscopy Society) meeting to be held in Raleigh, NC.The meeting dates are March 30 and 31. The theme for this meeting is "Recent Advances in Microscopy for 2000",specifically we would like to focus on recent (but not limited to) advances in microscopical instrumentation and applications therefrom.
Please forward any abstracts, papers or related items to me at mailto:\\rlmcgill-at-eastman.com or you may contact me at the number below.
The most recent meeting info is -at- http://www.wise.virginia.edu/cvc/arems/raleigh.html
Thanks in advance for your interest!
Rick McGill Microscopy Research Eastman Chemical Company - - - Phone: (423) 229-5473 - - - Fax: (423) 229-4558 - - - e-mail: rlmcgill-at-eastman.com
You should logon to Histonet - they're much friendlier!
Had a beautiful drive from old Plymouth to Reading, Berkshire, with my retired boss yesterday to collect x-ray microanalysis equipment. Clear blue sky, -2 to +5 degrees. Saw the most gorgeous scenes of trees covered by thick hoar frost - photographers' dream. Met two nice ladies - Hello, Jill and Hilary! Thanks for the help!
Keith Ryan Marine Biological Association of the UK PS - Don't read this if you don't like the weather ! PPS - See, Paul, I did get there! PPS - Hello, Daniele - time to write!
You ought to logon to "Histonet" - they're much friendlier!
I agree about the weather being important to EM. 20-30 years ago we had some seaweed hanging in the microtome room (about 100 m from the sea). If it was damp we didn't even try cutting some resins!
Had a car trip yesterday from old Plymouth to Reading, Berkshire, collect EM equipment., -2 to +5 degrees, clear blue sky, gorgeous scenes of thick hoar frost on the winter trees - a photographers' dream. Bonus - met two nice girls - Hello, Jill and Hilary, thanks for the help!
Keith Ryan Marine Biological Association of the UK PS - Don't read this if you don't like weather! PPS - Paul, I made it! PPPS - Hello, Daniele, its time you wrote!
Neither rain nor sleet, snow nor heat, humidity, hell nor highwater can keep me from getting Formvar films off glass slides. Only time can...see below.
My secret? Just rinse glass slides - both sides & all around edges, except for the end you are holding onto - with 95% ethanol and air dry. Then use right away - dunk into Formvar solution (.25 to .5% w/v in ethylene dichloride), drain and air dry. Score around edges to break film and float off onto clean water surface. I like to score the edge by using the corner of a razor blade to score on the top surface of the slide, near the edge, in addition to scoring the actual corner edge of the slide with the blade held perpendicular to the edge - know what I mean? Also, score across the slide near the "top" edge of the Formvar film, near the end you are holding on to, for clean release of the end of the film.
Second point, Formvar film solutions older that 3 months tend to stick to glass. I've been tracking that for years. Put mix date on bottle of fresh solution, after 3 months expect poor release effects to appear.
Gib
P.S. I tend to agree with Fook that this forum should not be used to discuss the weather ONLY, but if someome wants to put a current local weather "tag" at the end, AFTER the Microscopy stuff, with their signature, thats OK with me. Everyone is entitled to their (short) bit of poetry, sage sayings, or weather commentary there.
For example: "The weather here is unremarkable at this time."
Gib Ahlstrand Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu http://biosci.umn.edu/MIC/consortium.html
Dear All, I have a user who is trying to make TEM samples from nanophase metals. They come out of the process as a fine (10 to 100um) powder. The study is to compare the microstructure at different processing temperatures (77K to 400K). The current sample preparation technique is to embed the powder in epoxy, slice and polish, and finish with l-N2 ion milling (BTW, direct dispersion of the powder does not work as the edges are too thick). There are several problems with this technique, the worst being that we have found these materials age rapidly even at room temperature. The epoxy cure and ion milling thermal budgets may be a problem. I am considering ultramicrotomy for these samples, but I have zero experience in this field. McMahon and Malis (1995 Micro Res & Tech v31 267) worked on a similar system and outline the use of thermally cured LR-White as the embedding material, so I think it is an appropriate option, but I am worried about the thermal budget. My question is: Does any one have experience with low-temperature, UV cured resins for materials of these type? If so I would greatly appreciate any advice.
Thanks in advance, Ray
************************* Ray D. Twesten, PhD Center for Microanalysis of Materials University of Illinois (217) 244-6177 fax:(217) 244-2278
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Please see request below. If you have any suggestions, either send to the list and I will forward them to the investigator, or send them directly to Dale. Thanks for the input. Debby Sherman
--------------------------------------
Greetings, I can make two suggestions.
1) Ignore the staining in the cell wall. Since you know that your protein of interest is not there and you know also that secondary cell wall must be outside of the cell, this should not interfere with staning inside the cell.
2) Try to pre-absorb with "wood". The idea here would be to incubate your serum with some sort of wood pulp, and then spin out the wood pulp presumably taking with it all the ab's that bind wood, but leaving behind the ones of interst. I am not sure how best to make a suitable pulp of wood. Maybe take a pencil sharpener and grind a dowell, and then grind the shavings further in a mortar and pestle or maybe use a homogenizer of some sort. I am guessing wildly here. Certainly the wood bits should be easy to spin after absorption.
Hope this helps, Tobias.
} } Date: Thursday, January 27, 2000 } } From: Dale Karlson {dtk-at-omni.cc.purdue.edu} } } } } QUESTION: How to minimize artifact labelling with secondary cell walls } } } To whom it may concern: } } I am attempting to localize protein in a woody plant and consistently } observe an interaction with secondary cell walls. This is an artifact, we } know that the protein does not exist in the cell wall. We are using a } polyclonal antibody that was raised against a protein that was excised } from an SDS gel, suspended in Freund's complete adjuvant and used for the } immunization (in chicken). Chicken antibodies were purified by an } ammonium sulfate precipiation method described by Song et al. (Song, C.S., } J.H. Yu, D.H. Bai, P.Y. Hester, and K.H. Kim. 1985. Antibodies to the } 5-subunit of insulin receptor from eggs of immunized hens. Journal of } Immunology 135: 3354-3359). I have tried Western blot affinity } purification of the antigen and antibody and it has not solved this } problem. We do not have access to a "purified" form of the protein, so } affinity purification with a purified protein is not an option. } } It is obvious that the chicken had an "allergy" that was not visible } during our screening process (with western blots) to select the host } animal. The chicken obviously has specific antibodies to some secondary } wall component, and I would like to know what it might be and how I could } remove this artifact. } } Any input would be greatly appreciated. } } } ------------------------------------------- } } Debbie, } } let me know if this is suitable..or if it is way too long etc. } } Thanks, } } -Dale } } } } } } _______________________________________________________________________ } } Dale Karlson .***. .***. .***. } 1165 Horticulture Building * | | | * | | | * * | | | * } Purdue University * | | | * * | | | * * | | | * } W.Lafayette. IN 47907-1165 * | | | * * | | | * | | | * } '***' '***' '***' } } Home Phone: (765) 742-8379 } Lab Phone: (765) 494-1345 } _______________________________________________________________________
The decision has been made to get rid of the following piece of equipment. We obtained the unit approximately five years ago when an outside Contractor brought the system very near operational state. Numerous distractions and circumstances have prevented the TEM from becoming the valuable research investigative tool we had planned.
HITACHI H-600 TEM
1) Model H-600-1 Analytical Electron Microscope 2) Model H-6015 EDX Interface Kit 3) Model H-6012 Micro-Diffraction Unit 4) Spot Scan 5) Model H-6006 Auto Data Display Unit 6) Reduced Area Scan Unit 7) Polaroid Camera w/ Adaptor 8) Model H-6007 High Resolution CRT 9) Model H-5001-C Cobling Holder 10) 2 ea. Overhauled Mechanical Vacuum Pumps & 1 ea. Diffusion Pump
Anyone interested in requesting a Bid Form should contact me at following address:
Jim Goodman University of Tennessee Space Institute (UTSI) 411 B. H. Goethert Pkwy. Tullahoma, TN 37388 TEL: (931) 393-7494 FAX: (931) 393-7543 e-mail: jgoodman-at-utsi.edu
Yes, I also agree. Today in San Diego it is about 68 degrees with 76% humidity. Great for cryoultramicrotomy and regular cutting! Take care all, Jo Dee
Witold Zielinski wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } *Date sent: 26 Jan 00 17:52:26 -0800 } *From: Paul Webster {pwebster-at-hei.org} } *Subject: Re:No weather report please } *To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com} } *Send reply to: Paul Webster {pwebster-at-hei.org} } } *------------------------------------------------------------------------ } *The Microscopy ListServer -- Sponsor: The Microscopy Society of America } *To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } *On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } *-----------------------------------------------------------------------. } * } * } *} Please keep weather reports private. } *} } *} Ann Fook } * } *Why? A little light heartedness never hurt anyone. } *For the record, LA was sunny as usual today. Happy I don't live in CT anymore. } * } *Regards, } * } *Paul Webster, Ph.D. } *Associate Scientist & Director } *Ahmanson Advanced Electron Microscopy & Imaging Center } *House Ear Institute } *2100 West Third St. } *Los Angeles, CA 90057 } * } *Phone: (213) 273-8026 } *Fax: (213) 413-6739 } *e-mail: pwebster-at-hei.org } *http://www.hei.org/htm/aemi.htm } * } * } I agree with you Paul. } In Warsaw, Poland yesterday was snow on the ground today is } rain and no chance for sunshine. } Stay cool, } Witold
-- Jo Dee Fish Electron Microscopy Assistant The Burnham Institute 10901 N. Torrey Pines Rd. La Jolla, CA 92037 858-646-3100 ext.3620
This request is posted on behalf of an associate who does not subscribe to this list. He has assumed responsibility for the following equipment and is looking for service providers. Equipment is located in south central Massachusetts.
Jeol 840A sem Kevex delta 5 EDS & XRF with Syquest drive upgrade
He should be contacted off line by e-mail at LapradeB-at-burle-eo.com
FYI, I just tested an Optronics digital camera that had a on board firewire connection to a Gateway 366MHz notebook computer. One can also get PCMCIA card to connect to most notebook computers. Nice camera but should be for $13k, without notebook computer!
Damian
} 1394 (or firewire) is also a serial interface with a maximum bandwidth } of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast } enough for video. However, I believe that again more than one device can } be attached to a 1394 port which will again compete for bandwidth. } Firewire is not generally available on PCs (I believe the newer Macs } have it), so that for a firewire implementation on a PC normally a card } is necessary that fits into a PCI slot.
I'm trying to find a used ion beam sputter coater or something similar... The system I am used to is the old VCR group ion beam sputter coater. Any leads that you might have would be greatly appreciated...
Thanks,
Raj
********************************************* Raj Lartius, Ph.D. NovaScan Technologies Iowa State University Research Park 2501 North Loop Drive Ames, Iowa USA 50010
We are interested in acquiring an EDS detector for our JEOL JEM-1200EXII. Will trade a Kevex Be-windowed 30mm2 that was formerly attached to an EM400 for a Kevex/TN/Noran 10mm2/30mm2 Be or UTW. Need detector only (no MCA, P. Processor, etc). Will purchase or trade.
If you have a suitable detector and are in a position to trade/sell immediately, please reply off line to sender.
Bob Roberts EM Lab Services, Inc 2409 S. Rural Rd Tempe, Arizona 85282 (480) 967-3946
I have been reading about FRET and have a question about dual label imaging with probes like FITC/RHo or Cy3/Cy5. We have to worry about excitation of the long wavelength probe at the shorter probe wavelength, and FRET as two ways in which we can be mislead about the co-localization of two probes. I am wondering to what extent one also has to worry about non-FRET energy transfer. It seems that there is some possibility that, for example, Cy5 could become excited by absorbing photons from Cy3 emission. My presumption is that the density of photons is low, and this would limit the effect, but it seems that as proximity gets closer, the chances of this radiative exchange (rather than resonance exchange) would become greater. Are there any experimental guidelines as to when to worry about this? Thanks- Dave --
Dr. David Knecht Department of Molecular and Cell Biology University of Connecticut 75 N. Eagleville Rd. U-125 Storrs, CT 06269 Knecht-at-uconnvm.uconn.edu 860-486-2200 860-486-4331 (fax) home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html ************************************************************
On Mon, 24 Jan 2000 13:33:28 -0500, Greg Erdos wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Periodically questions appear on this list concerning the use of cryostats } for cutting histological sections. I usually reply to the sender off line } and offer a copy of a handout that I got from a workshop at a Histochem } Meeting some years ago. Even though it is old, cryostat sectioning has not } changed a lot. I think there is some valuable info there, especially for
} beginners. I thought it might be helpful to make this available on the net } for whomsoever might want to take a look. } It can be found at : } http://www.biotech.ufl.edu/sems/ } } Look for the snowflake } } It was written by Bruce Quinn, then of MIT. I hope he has no objections } to my posting it. } } Greg Erdos } Gregory W. Erdos, Ph.D. Ph. 352-392-1295 } Assistant Director, Biotechnology Program } PO Box 110580 Fax: } 352-846-0251 } University of Florida } Gainesville, FL 32611 } } } Finally got onto things here at home, and using Netscape 4.5 and Acrobat 3.0, the document opens up the way it should. Thanks for the info and link, Greg.
Roger C Moretz, Ph.D.
_______________________________________________________ Get 100% FREE Internet Access powered by Excite Visit http://freeworld.excite.com
A method I've used for controlling the position of the cleave in silicon wafers is to use focused ion beams (FIBs) to mill micro-cleaving grooves into the silicon. I found that the grooves can determine the position of a cleave to within 200 nm.
If you want any more details I can forward you a pre-print on the technique.
Richard
-------------------------------------------------------------- Richard M Langford
Department of Materials, University of Oxford Parks Road, Oxford, OX1 3PH, UK
----- Original Message ----- } From: Timothy Dimitri {tdimitri-at-us.ibm.com} To: {Microscopy-at-sparc5.microscopy.com} Sent: Friday, January 28, 2000 12:56 PM
Folks: I thought I should let you all know about the Second annual course in Quantitative Fluorescence Microscopy to be taught between june 19 and 24th 2000 at the Mount Desert Island Marine Biology Laboratories in Arcadia National Park in Maine. This team taught course led by Dave Piston (Vanderbilt), Mike Nathanson (Yale), Guillermo Romero (Pittsburgh) and myself focusses specifically on the development and application of modern fluorescence microscopic methods. This intensive course covers all aspects of the technology from microscope and dye design, cameras, confocal microscopy, live cell microscopy, multiphoton microscopy and GFP. Considerable attention is also given to quantitative analysis in 2 and 3 dimensions and time. The specific focus of the course allows an in depth treatment of these methods. The goal of the course it to teach students how to best implement these methods within their labs, using either their own cells and tissues or using material supplied by the course. An extensive array instrumentation, provided by all the major microscope and associated software, hardware and camera manufacturers will be available for students to use. Last year it was a very successful event and we were encouraged to give the course again. A full description of the course lectures together with lecture outlines, registration etc. is available at http://sbic6.sbic.pitt.edu/microscopy. I would encourage you to spread the word, or sign up if you are interested. The total number of students is limited to 20, enrollment is decided by the course faculty. If you have any further questions please feel free to contact me Thanks Simon
----------------------------------------- Simon C. Watkins Ph.D. MRCPath Associate Professor Director: Center for Biologic Imaging University of Pittsburgh Pittsburgh PA 15261 tel:412-648-3051 fax:412-648-8330 URL: http://sbic6.sbic.pitt.edu ----------------------------------------- Simon C. Watkins Ph.D. MRCPath Associate Professor Director: Center for Biologic Imaging University of Pittsburgh Pittsburgh PA 15261 tel:412-648-3051 fax:412-648-8330 URL: http://sbic6.sbic.pitt.edu
At 1:09 PM -0500 1/29/0, "IMZartTchr-at-aol.com"-at-sparc5.Microscopy.Com wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
*********** If you don't get an answer from MSA people, try the listserver for the histologists out there: "HistoNet Server" {HistoNet-at-Pathology.swmed.edu}
Lee
Lee Cohen-Gould EM & Confocal Facilities Weill Medical College of Cornell U. (212)746-6146 Rms A-105, LC-207
The Cape Town weather is great today, beautiful clear blue skies and a nice warm 25 C.
We have a Philips TEM 420 which has an EDAX system attached (which is non-functional at the moment). We are looking for video / digital image grabbing system that we can use to grab images for prints and possibly Image analysis. Is this possible on the 420 ? Can anyone suggest a system?
A graduate student here (Rita Ware) had some success fixing halophilic bacteria for TEM several years ago. She used the growth medium as a buffer. She made a poster presentation at an MSA meeting.
} Date: Mon, 24 Jan 2000 06:46:26 -0700 } From: Marti, Jordi {jordi.marti-at-honeywell.com} } To: 'Microscopy' {Microscopy-at-sparc5.Microscopy.Com} } Subject: Reichert Ultracut E with cryo FC 4D } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } The power supply in our cryo microtome is having problems which might be } related to the transformer. I was told by the service engineer that the } transformer is no longer supported by Reichert. Does any one know where I } can get a replacement ? } } Thanks } } Jordi Marti } }
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
South Bay Technology, Inc. manufactures an SEM Cleaving System which provides a means to precisely and quickly cleave a wafer while in the inspection mode. A wafer is mounted to a vacuum chuck which is positioned under an optical microscope. The exact area of interest is located visually and the sample is cleaved at that point. SEM compatible versions of the cleaving system are under development which will allow the user to image and cleave while mounted in the SEM. The Cleaving System is quick, easy to operate and precise. It allows anyone to quickly and repeatably prepare SEM cross sections.
If you have an interest, please let me know and we can discuss your requirements in detail.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by Timothy Dimitri } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hello,
I am looking for the names of any manufactures (other than SELA) that have a product that can cleave sub micron features on silicon wafers...
Thank you
Timothy Dimitri ASTC Failure Analysis Laboratory IBM Microelectronics Division
I forget to mention in my original posting that South Bay Technology also produces the MicroCleave kit which is designed for TEM cross sectioning. Again, if you have an interest, please let me know and I'll get you additional information.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy. Message text written by Timothy Dimitri } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hello,
I am looking for the names of any manufactures (other than SELA) that have a product that can cleave sub micron features on silicon wafers...
Thank you
Timothy Dimitri ASTC Failure Analysis Laboratory IBM Microelectronics Division
While I don't have any leads on a used IBS system, I wanted to let you know that we at South Bay Technology, Inc. are continuing the manufacture of the IBS system formerly produced by VCR Group. Actually, we have updated the system and are now offering the IBS/E. The IBS/E now adds the capability of etching samples as well as coating and will accommodate samples up to 2" in diameter.
If you would like additional information, please feel free to contact me.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by "Dr. Raj Lartius" } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I'm trying to find a used ion beam sputter coater or something similar... The system I am used to is the old VCR group ion beam sputter coater. Any leads that you might have would be greatly appreciated...
Thanks,
Raj
********************************************* Raj Lartius, Ph.D. NovaScan Technologies Iowa State University Research Park 2501 North Loop Drive Ames, Iowa USA 50010
Are we like kids sitting in a sandbox and squabbling? Yes weather, no weather! He said it first! She started it! I don't want weather on the listserver. I want to read about weather in the boondocks. Who cares about you, anyway? Sort of funny when you think of it that way, isn't it?
A friend has the chance to buy a used JEOL JSM840 - AM1000 with EDX and all the bells and whistles. He has asked if this is a good instrument, and worth the price (whatever that is - he won't tell me). Since I haven't seen the instrument and I don't know JEOLs at all, I told him I would ask the experts.
If anyone can tell me a little about the instrument and what it might be worth (the second part of the question being more difficult), I would appreciate it.
In Honolulu it is gloriously clear and sunny, with temperatures near 80F during the day and about 60F at night, which is unusually cold but really nice.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
You are invited to participate in a symposium of remote access microscopy, and /or teaching microscopy to take place at the Microscopy & Microanalysis Annual Meeting August 13-17, 2000 in Philadelphia, Pa.
Platform and poster contributions are welcome. Please contact me directly for more information about the symposium.
Deadline for receipt of a 2page abstract is Feb 15, 2000. For registrationand abstract forms, see http://www.microscopy.com/MSAMeetings/MMMeeting.html
ADVANCES IN INSTRUMENTATION AND TECHNIQUES SYMPOSIUM 19: TEACHING MICROSCOPY IN THE NEW MILLENNIUM
Organizer: Steve Barlow
The use of computers to control microscope operations, the ability to control microscopes remotely over the Internet, and the creation of microscope computer simulations allow researchers and students to access microscopes in new ways. These developments mean changes in the way microscopy can be taught to students and researchers. This symposium will examine different ways to teach microscopy and microscope theory and operation to reseachers and students of all levels, in the context of new laboratory configurations, computer simulations, remote access usage, and classroom exercises.
Dr. Steven Barlow, Associate Director EM Facility/Biology Department San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619)594-4523 fax: (619) 594-5676 email: sbarlow-at-sunstroke.sdsu.edu website: http://www.sci.sdsu.edu/emfacility/
Chairman, Educational Outreach subcommittee promoting access to microscopes Microscopy Society of America http://www.msa.microscopy.com/
We have a new (to us) Philips CM-12 TEM in our lab and are wondering how to get the intermediate lens focused on the diffraction aperture (for making the first image plane and the diffraction aperture coincident prior to obtaining a SAED pattern). It doesn't seem to be covered in the manual.
Any help would be appreciated as this is a completely new microscope to us.
The JEOL 840 is an excellent instrument: very reliable & very easy to use. Enjoy with confidence.
Earl Weltmer
P.S.: Does your friend need someone to install the SEM? I would love to install it assuming it is in Hawaii.
Tina Carvalho wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi, microscopists- } } A friend has the chance to buy a used JEOL JSM840 - AM1000 with EDX and } all the bells and whistles. He has asked if this is a good instrument, } and worth the price (whatever that is - he won't tell me). Since I } haven't seen the instrument and I don't know JEOLs at all, I told him I } would ask the experts. } } If anyone can tell me a little about the instrument and what it might be } worth (the second part of the question being more difficult), I would } appreciate it. } } In Honolulu it is gloriously clear and sunny, with temperatures near 80F } during the day and about 60F at night, which is unusually cold but really } nice. } } Aloha, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } ****************************************************************************
Hello All, Does anyone know of a supplier for Historesin, formerly Cambridge, Leica, LKB? And, the weather in SoCal is quite lovely today - it finally rained. Thanks, Kristen Kristen A. Lennon Cell, Molecular & Developmental Biology Group Department of Botany & Plant Sciences University of California Riverside, CA 92521 kalen-at-citrus.ucr.edu
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Hi,
Are we like kids sitting in a sandbox and squabbling? Yes weather, no weather! He said it first! She started it! I don't want weather on the listserver. I want to read about weather in the boondocks. Who cares about you, anyway? Sort of funny when you think of it that way, isn't it?
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Would you be interested in a Zeiss 9s2 TEM? 60k is the top magnification. It has been a nifty scope as we have upgraded to a Zeiss 109. If you are interested let me know. Cheers! -Ken ------------ Ken Tiekotter Dept. of Biol. The University of Portland 5000 Willamette Blvd. Portland, OR 97303
On Wed, 26 Jan 2000, Cynthia Shannon wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Subject: Wanted: Used TEM for virus work } Date: Tues, 26 Jan 2000 } } From: cshannon-at-nctimes.net } To: Microscopy-at-sparc5.microscopy.com } } Does anyone have an old TEM for virus work? } I am the electron microscopist for the county veterinarian. We are short } of funds. Please contact me by email. } Thanks. } Cindy Shannon } } }
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