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BOC Gases, has a unique opportunity for an Electron Microscopist/Materials Engineer located at it's state of the art Technical Center in Murray Hill, NJ.
BOC Gases, a division of The BOC Group Inc., was founded in 1888 and is a global leader in the manufacturing and distribution of industrial, medical, and specialty gases and related equipment. Poised for new growth opportunities, BOC currently operates in more than 50 countries, providing customers with gases and technology which are used to support life, freeze food, forge steel, shape glass, refine petroleum, create computer chips, and clean wastewater and smokestack emissions, in addition to hundreds of other applications.
Electron Microscopist/Materials Engineer
Responsibilities
The successful candidate will support the materials characterization needs of the Gases Technology R&D organization using and maintaining analytical equipment, such as, Scanning Electron Microscopy, X-ray Diffraction. Metallography. Characterization includes metallic surfaces, adsorption/catalysis materials as well as other materials related to gases delivery equipment and vessels. This position is within the analytical services part of the Materials Technology Group. More specifically, the person will have the responsibility to:
Provide scanning electron microscope (SEM), environmental scanning electron microscope (ESEM), and metallography services.
Maintain the SEM and ESEM equipment in good operating condition.
Develop new techniques to support researchers needs.
Perform failure analysis and provide technical assistance for material selection projects.
Experience/Education
A B.S. or M.S. in the field of materials science and engineering with practical working experience with scanning electronic microscope techniques. Experience in x-ray diffraction a plus.
A knowledge of a wide variety of materials. Experience with mechanical testing, failure mechanisms, corrosion, and materials, especially metals and polymers. 5-10 years work experience in a materials laboratory.
Work experience in the chemical processing industry a plus.
Ability to perform failure analysis.
Ability to multitask is essential.
Send application to: BOC Gases, Attn: Recruiting - SEM-NM, 575 Mountain Avenue, Murray Hill, NJ 07974. E-mail: jobs-at-us.gases.boc.com
fax: 908-771-1702. Along with opportunities for professional growth we offer competitive compensation and comprehensive benefits.
BOC Gases is an equal opportunity employer.
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When I had resolution problems recently (which turned out to be due to a "leaky" detector) one of the first things my service engineer asked me to check was to make sure that the detector wasn't touching the pole piece (which can cause a ground loop?) Try backing the detector out a little to see if the peak width changes. I doubt it will help, but it is easy to try. Matt
"E. J. McKenzie" {elizm-at-pdx.edu} on 07/28/2000 12:30:59 PM
To: microscopy-at-sparc5.microscopy.com cc:
Hi everyone,
we are having serious problems with the resolution of our EDS detector - the peak height-width ratio is terrible, around 450 counts. Consequently, our peaks look more like rolling hills and one peak blurs into another. I have callibrated the machine using an Al-Cu standard, checked that the detector is kept cool, sample is tilted at 30 degrees, etc etc. I also got another microscopist to come and have a look to check my methods and he also concluded there was a serious problem.
Is there anything else I could try before I phone the Noran technicians and pay them a lot of $$$ to fix/replace the detector?
regards Liz McKenzie -------------------------------------------------- Geomicrobiology and Electron Microscopy Laboratory Room S9 Cramer Hall 1721 SW Broadway Portland State University Portland OR97201
Folks does anyone know where I could obtain a service manual (not a user manual) for a Microm 505 cryostat Tx Simon
---------------------------------------------- Simon C. Watkins Ph.D. MRC Path Associate Professor Cell Biology and Physiology Director Center for Biologic Imaging University of Pittsburgh BSTS 225 3500 Terrace ST Pittsburgh PA 15261 Tel:412-648-3051 Fax:412-648-8330 URL: http://sbic6.sbic.pitt.edu --------------------------------------------
We manufacture semiconductors. I work in the Analytical Services cross-sectioning these daily. These images you sent are of a bulk silicon process. To best describe the layers, I will start at the silicon and work my way up. On the second picture the field oxide comes to a point in two locations allowing the metal above to make contact to the silicon. The first picture shows this layer as the full thickness along the silicon. The next layer is the 1st metal(not poly silicon) that is making a contact in the second image and just a first metal line on the first image. The metal has a undoped oxide over it that gives the different z contrast. Then there is an oxide over that, could be PSG. Then there is a second metal layer(labeled Poly Si on the first picture). On top of that is a layer of oxide(which is labeled Al on the first picture). Then on top of everything is either a nitride or polyimide capping the chip. The metals are easy to identify because of the grain structure. There are not any gates shown on these two photos. Gary if you would like to give me a call I can explain the layers in more detail. I can also give you information on how they are processed and what to look for.
Mark Windland Honeywell Solid State Electronics Center Plymouth, Minnesota 612-954-2845 ------------------- } Thanks very much to all who have responded to this initial } posting. It seems to me that the data I have received confuses } me for than before. But the ideas presented are great to } consider. Proving them out is quite another matter. } } At 09:14 AM 7/30/00, you wrote: } } Gary, } } } } It is common for IC manufacturers to use metals in direct contact with } poly } } gates thus reducing the sheet resistance of the poly especially for } } interconnects such as in your image. It could be that the similar } contrast } } means a titanium-silicide or something like that. Therefore, to excite } the } } higher x-ray lines will require higher beam voltage and you are correct } in } } wondering about the volume which is generating the detected x-rays. } } } } I have had much better success analyzing semiconductor cross sections } with } } Auger than EDS. Auger's built-in ion gun sputtering provides a good } means } } of cleaning the face of the cross section, the resolution (for imaging } and } } analyzing) is certainly adequate for this size structure and once you } have } } identified the elements present over an area, a line scan nails the } elements } } present in the gate. } } } } I have some question about the labels on the photo. Are you really sure } } about those? } } } I offer a second image at } } http://photoweb.net/icxsect2.jpg } } which is a different and augmenting image of the IC structure. } } I am not sure of the identification of each layer but I am } pretty sure of them. However, I can be dissuaded by alternate } opinions. The main point here is I think to keep in mind } that this is a fifteen year plus construction...it is not modern. } Therefore, what would be vogue or state-of-the-art back then? } } The ability of x-ray analysis to quantify the various layers } seems to be easy or impossible, based on the feedback I } have received. On first blush, it does seem difficult based } on the small geometric area available for probing. I do not } yet have an x-ray capability but will be receiving one soon. } It is a 133eV light element dewar system. It will be interesting } to see how it performs in this application. } } gary } }
Does anyone out there have a Kevex-ray EDX detector, 2003 model? We don't have a manual (or any literature, for that matter) and I just wondered if I could obtain a photocopy or acrobat file from someone. I've also contacted Noran.
Thanks for all the hints on fixing the peak problems - it seems to be working fine now (210eV FWHM for Mn peak), I just have to figure out what went right!
regards Elizabeth McKenzie -------------------------------------------------- Geomicrobiology and Electron Microscopy Laboratory Room S9 Cramer Hall 1721 SW Broadway Portland State University Portland OR97201
I did not comment on your last image, as I needed more info. before making a statement. The second image is easy to comment on. The gate structure is the line which is the same contrast as the substrate. It starts towards the left of the image, over "field oxide", drops down over the "birds beak" and continues across the gate oxide. Then it rises over the other "birds beak", and terminates over the "field oxide". The darker thin line at the surface of the substrate (connecting the two "bird beaks") is the gate oxide. The gate appears to be polysilicon, but I would need more information to say for sure.
I am having problems removing a white, spotty, crystalline- like residue from my XTEM sample. Has anyone out there experienced similar problems? Any suggestions would be appreciated. I summarize my prep procedure below, as well as the methods I used to try to remove the residue. (It should be noted that my sample is a sandwich sample prepared using Gatan G-1 epoxy.)
1. Mount sample on pyrex polishing stub with superglue. 2. Polish with alumina lapping films. 3. Final polish with polycrystalline diamond suspension on a final polishing cloth. 4. Soak in acetone to dissolve superglue and release sample from stub.
After the above procedure there was the aforementioned residue. I attempted removing it by the following methods: 1. Rinsed/soaked in ethanol. 2. Rinsed/soaked in water. 3. Rinsed/soaked in acetone overnight. 4. Rinsed/soaked in hexane. 5. Sonicate in acetone. 6. Sonicate in water.
} From time to time we have recommended cyanoacrylate super glues for use with tripod polishing and related methods. The trouble with using consumer products is that they sometime change without warning. We have experienced poor results with glues we have recommended in the past. No point in naming names. After performing an evaluation on replacement glues, we now recommend:
1). RP30 CYANOACRYLATE ADHESIVE - $6.75/1 oz. bottle
by AMERICAN CHEMICAL CO. 2630 South Irving Avenue Minneapolis, MN 55408-1047 (612)-374-1767 Contact: Bill Reker FAX: (612)-377-3590 Web: www.glueit.com E-mail: info-at-glueit.com
2). 310035 INSTANT SUPERGLUE ETHYL CYANOACRYLATE - $5.98/1 oz. bottle
by ND INDUSTRIES 750 Wylly Avenue, Suite 3 Sanford, Fl. 32773 (800)-521-2663 Contact: Laurie Russell
Sold to us via: Moreau Marketing & Sales 501 Shepherd Street, Suite #4 Winston Salem, NC 27103 Contact: Robert Moreau (336)-659-6674 FAX: (336)-659-6683
For further details, please contact Mark Hudson at (845)-892-2441 (currently using RP30) or Stan Klepeis at (845)-892-2192 (currently using 310035).
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
IBM Analytical Services; http://www.chips.ibm.com/services/asg
It seems as if you may be contaminating your sample with the superglue after using the acetone to clean the sample. However, what you describe sounds much like colloidal silica particles.
Try using IPA or methanol after acetone. Acetone leaves residue on samples and does not come clean easily.
If for some reason you neglected to mention the use of colloidal silica, I am 98 percent sure that is what you see. If that is the case, MicroOrganic Soap works well to remove colloidal silica from samples. You may also wish to switch the type of colloidal silica you are using.
I hope this works for you.
Sincerely,
Gary Liechty Manager, Technical Products
Allied High Tech Products, Inc. 2376 E. Pacifica Place Rancho Dominguez, CA 90220
310-635-2466 310-762-6808 Fax 800-675-1118 Toll Free, US and Canada
www.alliedhightech.com
Equipment and Consumables for Metallurgical Sample Preparation ----- Original Message ----- } From: "john david whitaker" {jwhitake-at-u.washington.edu} To: "MSA listserver" {Microscopy-at-sparc5.microscopy.com} Sent: Tuesday, August 01, 2000 10:39 AM
Dear Microscopy Listserv,
I have a colleague that is doing a light microscopic comet assay (single cell gel electrophoresis) on glass slides using respiratory cells from sputum. She has been using silver staining (w/ a kit from Trevigen) and is having difficulty getting clean preps (ie without precipitates or extraneous staining). Does anyone have any tips or suggestions? She's tried doing the assay with fluorescent preps on our confocal microscope, but the specimen fades too quickly to acquire useful data.
Yours, Doug .................................................................... : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy : : Research Specialist, Principal University of Arizona : : (office: AHSC 4212A) P.O. Box 245044 : : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu): :...................................................................: http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW"
Arizona State University Center for High Resolution Electron Microscopy Research Specialist
The Center for High Resolution Electron Microscopy at Arizona State University has an open position for a Research Specialist. The Center provides electron microscopy resources for the university and external community, including a range of transmission and scanning electron microscopes, as well as specimen preparation facilities. The successful applicant will be expected to assist with the day-to-day operation and maintenance of microscopes within the Center. Primary instrument responsibility will include but not be limited to the JEOL 2000FX transmission electron microscope (TEM), the JEOL 840 Scanning EM, and the soon-to-be acquired JEOL 6300 SEM with liquid-helium CL system. Other tasks will include training and supervision of researchers in proper use of the instruments, including observance of safety procedures, and authorization of users. Some limited opportunity to assist and advise researchers in designing and carrying out experiments, including active participation and collaboration in experiments with data collection and analysis as required.
Minimum qualifications: Bachelors or Masters degree or equivalent training in Physical or Materials' Sciences or closely related discipline, with at least three but preferably five years' additional experience with operation and maintenance of scanning and/or transmission electron microscopes. Experience with design, construction and testing of electronic circuitry, and knowledge of vacuum systems would be desirable.
Application deadline: August 15, 2000, or until position filled.
Arizona State University is an equal opportunity employer. Women and minorities are encouraged to apply.
Send resume and the names and addresses of three references to John Wheatley. Please contact John via one of the following ways--preferably e-mail:
John C. Wheatley, Lab Manager Arizona State University Center for Solid State Science Tempe, AZ 85287-1704
Thanks. Gen ****************************************************************** Gen Pei Department of Materials Science and Engineering Cornell University 328 Thurston Hall tele: (607)255-5177 fax:(607)255-2365 gp35-at-cornell.edu
| ------------------------------------------------------------------------ | The Microscopy ListServer -- Sponsor: The Microscopy Society of America | To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com | On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html | -----------------------------------------------------------------------. | | | Thanks. Gen | ****************************************************************** | Gen Pei | Department of Materials Science and Engineering | Cornell University | 328 Thurston Hall | tele: (607)255-5177 | fax:(607)255-2365 | gp35-at-cornell.edu | | ****************************************************************** |
Nelson Conti 164 Ferne Court Palo Alto, CA 94306
Gen Pei- I've unsubscribed myself one time. I believe that you need to send an e-mail to the MSA Listserver (address = ? ), and just indicate "unsubscribe" in the subject heading. To find the address, go to the MSA Listserver Web address, and it should be possible to find the e-mail address for the MSA Listserver. Sorry, I don't recall it off-hand. Good luck with unsubscribing. Nelson Conti P.S. When in doubt - you can also re-send your message, but direct it to Nestor Zaluzec. I'm sure that he can tell you precisely how to unsubscribe.
_______________________________________________________ Say Bye to Slow Internet! http://www.home.com/xinbox/signup.html
Have any of you had any experience with the installation of a wireless lan affecting the operation of your microscopes in any way? I would very much appreciate your advice in this regard.
Sincerely,
Mick Thomas ------------------------------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
I have not yet seen any effects, with the Apple Airport system installed for my computers as well as a GigaHertz wireless broadcasting systems which are carrying video signals from one room to another.
The Computer monitors however are a different story.
Nestor
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Sorry for the previous blank message. Nestor thinks it was just a fluke.
We have an Astromed cooled CCD camera, which we would like to use. However, we have no manuals for it. If you have manuals or other information that would allow us to put this camera into operation please let me know "off line".
Thank you, Alwyn Eades .
The camera has two labels: Model number TE2W (serial 117) Model number P86121 (serial 7451/8/14)
There are two boxes of electronics labelled: Box 1 Model TE2/PSU (serial 102) Box 2 Model DDE/2200 (serial 116) Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvannia 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
Hello everyone; I have just started looking at a GFP-transformed fungus growing on plant surfaces, using regular fluorescence microscopy. I can clearly see the fungus using two filter sets: a UV set (ex/em 365/} 420) and a blue filter set (ex/em 470/} 515). While the fluorescence in blue light is much brighter, stuff that is blue/blue-white with UV illumination is green in the blue light. is the "extra" green in the blue light likely to be where the GFP is not as concentrated, and hence doesn't show up in the UV, or is it more likely to be some other compound that has similar excitation/emission characteristics? I have done a fair bit of reading on the subject, but am still feeling a bit vague, and would really appreciate hearing from those of you who have some first hand experience with GFP.
thanks in advance shea
Dr. S.Shea Miller Agriculture & AgriFood Canada Eastern Cereal and Oilseed Research Centre Rm. 2068 Neatby Building Central Experimental Farm Ottawa, Ontario Canada K1A 0C6 Phone: (613) 759-1760 Fax: (613) 759-1701 E-mail: millers-at-em.agr.ca
With regard to the posting I made on this subject, Chuck Garber sent me the following note wherein he raises a number of important points. Chuck is correct in all regards. The product we were using was not DURO. We have no experience with Duro. We have made specific, brand name, recommendations in the past and my note was to share our recent experiences regarding these products. The products we were using were not "garage level" products.
So, IF, and only IF, you are using a product solely because of a recommendation from our lab, we now make new recommendations.
Ron
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
Hi Ron,
I guess I should give some kind of disclaimer statement first, that in no way am I suggesting that what you are saying it not correct. If this was your perceived experience, then I am sure it is correct and I am not questioning it.
And it is a fact that there are some garage level operations where people take from 5 gallon pales and "fill" the 1 oz plastic bottles and put on a label and put it into distribution, without any real testing of the integrity of their packaging or any of the other things you and I might think one should be doing to insure the integrity of their product.
But I can assure you, without hesitation, the cyanoacrylate that is made by Duro, which is indeed a consumer products company, but it is also "top in class" when it comes to this type of product, has been offered by us (e.g. SPI) for years and we have never ever had anyone every voice even the slight complaint about it. We also decided a long time ago that once opened, the shelf life of these products can be very short, and consequently, we decided to offer the product in the smaller 3g package.
I go through the trouble to point this out to you because I see things from a slightly different perspective possibly than you do:
1] For one thing, someone even at IBM, having to place an order with either of the two mentioned companies, given the cost of placing an order period of any size, what is the overhead going to be? But if someone was ordering the Duro product from someone like SPI, then it just gets added in with a bunch of other things and the real cost is almost nothing.
2] For someone in Mexico, the situation is far worse. You have a lot of influence on people because of your reputation. Your posting could make someone in Mexico City think that in order to get acceptable results, they have to place a direct order in the USA for one of these "special" cyanoacrylates and in situations with limited research budgets, it just seems to me like they have better ways to be spending their money.
So while the Duro product might not be any better than the two mentioned, the point is that because of the distribution and transportation costs, if people are led to believe that they really do need something that can not be procured from their traditional sources of purchasing, they end up wasting a lot of money unnecessarily.
Of course if you had any hard evidence that the Duro product was in some way inferior, that would surely be another story. But chemically, these are all the same identical materials, they all have the same identical MSDS information and the all perform identically. When ever I have heard of someone having problems it has been because they were using product from a previously opened package.
Feel free to use this information however you feel might be approriate, either in whole or in part. But I did want to share my views with you on your posting, which I hope you won't mind my having done.......
Chuck
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
IBM Analytical Services; http://www.chips.ibm.com/services/asg
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Hi all, We would like to add an individualized computer password system to our FEI/Philips CM-10 TEM. We would like a user to be able to type in their password which would then trigger a switch tied into the high tension and turn the HT on. The system would ideally then have a timer to record "on" time and a function to record this time with the user ID. Connecting a timer to the high tension is not a problem...the computer password part is where we need help. Please contact me if you have a system such as this on a FEI/Philips CM-10 or even on another make TEM which might work on a CM-10. Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
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Date sent: Wed, 02 Aug 2000 11:20:54 -0400 } From: "Shea Miller" {millers-at-em.agr.ca} To: confocal-at-listserv.acsu.buffalo.edu, microscopy-at-sparc5.microscopy.com
Well, I guess Mark and I would have to see a higher magnification image, detailing the polysilicon, the "birds beak" area, and the substrate. This way we could tell whether this is a gate, with gate oxide, or a diffusion contact. I am assuming that your cross section is well into the middle area of the polysilicon line, and not just at the edge.
OK...I think that I have this figured out. I got some good input from several folks who were right about some portions and some who were perhaps wrong. So was I. But I think that I have it sorted out now.
I really can't say that this is a double poly structure quite yet until my x-ray system is available (Rontec 133eV SHV). Even then, it may not be able to discern the constituents in the small gate area. We'll see.
In the meantime, I will put an annotated pix on my site which shows what I think is the real identification of each layer. It still may not be 100% right....but I think that it will be quite close.
I am looking for a programme (or a collaboration) to compute powder XRD or SAED taking in account the size effect due to the platelet shape of small crystals in a polycristalline (non textured?) powder (hexagonal Bravais lattice).
In a first, simple, approach we can consider only the beam broadening of the bulk reflections (‰+/size in that particular direction). A more accurate computation would take in account that the smallest crystals are no longer a sum of complete unit cells but a supercell.
Simulation of powder patterns with CrystalMaker or Carine show that it exist a strong overlapping between reflections for isotropic crystal some nanometers in size.
Do somebody know if such a programme is available or where somebody could perform a few simulations for me?
Best regards
Philippe Buffat
__________________________________________________________________ Philippe Buffat Ecole Polytechnique Federale de Lausanne (EPFL) Centre Interdepartemental de Microscopie Electronique Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time) E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/ ______________________________ Eudora F2.1 ___________________________
As we approach the upcoming meeting I would like to take this opportunity as the LAC Chair to let you know that this meeting promises to be one of the best yet. With a sell out of Exhibit space and pre registration growing Philadelphia is the place to be this August. To all of our surprise, we have sold out on most social events. We do have left just 75 Spirit of 76 Wednesday Evening Dinner Cruise tickets left which we would like to open up to those of you whom have not purchased any. Joining this trip will afford you the relaxing experience of floating down the Delaware River on an elegant ship filled with wonderful food, and lively music. Sail by Penn's Landing, Old Christ's Church, the Ben Franklin Bridge, the Flagship Olympia, and much much more. The cruise includes a five entry buffet with all of the condiments, salad, desserts and coffee and tea. There are 2 dance bands as well as entertainment on board. Transportation will be from all of the local hotels and convention center. All of this on a usually boring Wednesday night for only $55 for everything. Please E-mail me directly or call if you are interested so we may make all of the arrangements. I look forward to hearing from you.
Our university is looking to do some imaging of DNA and DNA/protein interactions. We do not have a AFM and are looking to contact anyone in the NJ, NY, CT, or PA area so that we could visit a lab and possibly contract out some work or work in collaboration. If you are aware of any company/university that has AFM, could you please contact myself or Jack Gaynor at these email addresses: mganger-at-aol.com or jack.gaynor-at-montclair.edu.
Thanks in advance,
Mike Ganger Montclair State University mganger-at-aol.com
Dear Sir/Madam: Do you know of any vendors who sell older or discontinued Nikon parts? I'm looking for a dic nosepiece for a diaphot 200? Any suggestions. TIA
This message is in reply to Philip Oshel at U. of Wisconsin:
I've always been told that the brown goop that is easy to get on your hands while pulling apart the photo & negative of the Polaroid T55 film is a known carcinogen and to wash your hands immediately upon contact.
Jane L. LaGoy Development Engineer Bodycote IMT, Inc. 155 River Street Andover, MA 01810 978-470-1620 jlagoy-at-bodycote-imt.com
I would like to ask you how to stain non-collagenous fibres in paraffin-embedded human tissues (i. e. liver), by using Fast Green stain. Have someone the method?
I read some paper about this stain, but these reported a pre-incubation with 0.01% Fast green in saturated picric acid solution for 15 min, and then after some washes a new incubation with Fast green at the concentration of 0.04% in saturated picric acid solution for 30 min.
Why is important the pre-incubation?
Many thanks, Fabio Grizzi
Dr. Fabio Grizzi Direzione Scientifica, Istituto Clinico Humanitas Via Manzoni 56 20089 Rozzano, Milan, Italy
Curious. I've never heard this, but I won't disagree. It's photodeveloper chemicals, same basic sort of stuff used in photography darkrooms. I haven't seen any warnings about these chemicals in the packaging materials/information sheets. It is common sense to keep it off your hands, and to wash your hands if you get it on them, but I haven't had any problems not getting the goop on my hands. And I'd have several trophies for clumsiness if I hadn't dropped and broken them all ...
Phil
} This message is in reply to Philip Oshel at U. of Wisconsin: } } I've always been told that the brown goop that is easy to get on your hands } while pulling apart the photo & negative of the Polaroid T55 film is a known } carcinogen and to wash your hands immediately upon contact. } } Jane L. LaGoy } Development Engineer } Bodycote IMT, Inc. } 155 River Street } Andover, MA 01810 } 978-470-1620 } jlagoy-at-bodycote-imt.com
-- }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{ Philip Oshel Supervisor, AMFSC and BBPIC Dept. of Animal Health and Biomedical Sciences University of Wisconsin 1656 Linden Drive Madison, WI 53706-1581 voice: (608) 263-4162 fax: (608) 262-7420 (dept. fax)
The MSDS on the 55 developer states that the developer does not contain any listed carcinogens by OSHA, IARC or NTP. The major danger seems to be the corrosiveness of the alkali from Potassium and Lithium hydroxide. Ingestion obviously is a danger due to these and other components. Polaroid has it's MSDS's up on it's website at:
http://www.Polaroid.com/service/msds/index.html
Gloves and/or handwashing are very much in order.
} I've always been told that the brown goop that is easy to get on your hands } while pulling apart the photo & negative of the Polaroid T55 film is a known } carcinogen and to wash your hands immediately upon contact.
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Date sent: Wed, 02 Aug 2000 11:20:54 -0400 } From: "Shea Miller" {millers-at-em.agr.ca} To: confocal-at-listserv.acsu.buffalo.edu, microscopy-at-sparc5.microscopy.com
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Fellow microscopists,
Have any of you had any experience with the installation of a wireless lan affecting the operation of your microscopes in any way? I would very much appreciate your advice in this regard.
Sincerely,
Mick Thomas ------------------------------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
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I have not yet seen any effects, with the Apple Airport system installed for my computers as well as a GigaHertz wireless broadcasting systems which are carrying video signals from one room to another.
The Computer monitors however are a different story.
Nestor
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Fellow microscopists,
Have any of you had any experience with the installation of a wireless lan affecting the operation of your microscopes in any way? I would very much appreciate your advice in this regard.
Sincerely,
Mick Thomas ------------------------------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
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Well, I guess Mark and I would have to see a higher magnification image, detailing the polysilicon, the "birds beak" area, and the substrate. This way we could tell whether this is a gate, with gate oxide, or a diffusion contact. I am assuming that your cross section is well into the middle area of the polysilicon line, and not just at the edge.
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On Tue, 1 Aug 2000 19:09:28 -0400, Gen Pei wrote:
| ------------------------------------------------------------------------ | The Microscopy ListServer -- Sponsor: The Microscopy Society of America | To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com | On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html | -----------------------------------------------------------------------. | | | Thanks. Gen | ****************************************************************** | Gen Pei | Department of Materials Science and Engineering | Cornell University | 328 Thurston Hall | tele: (607)255-5177 | fax:(607)255-2365 | gp35-at-cornell.edu | | ****************************************************************** |
Nelson Conti 164 Ferne Court Palo Alto, CA 94306
Gen Pei- I've unsubscribed myself one time. I believe that you need to send an e-mail to the MSA Listserver (address = ? ), and just indicate "unsubscribe" in the subject heading. To find the address, go to the MSA Listserver Web address, and it should be possible to find the e-mail address for the MSA Listserver. Sorry, I don't recall it off-hand. Good luck with unsubscribing. Nelson Conti P.S. When in doubt - you can also re-send your message, but direct it to Nestor Zaluzec. I'm sure that he can tell you precisely how to unsubscribe.
_______________________________________________________ Say Bye to Slow Internet! http://www.home.com/xinbox/signup.html
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Hello everyone; I have just started looking at a GFP-transformed fungus growing on plant surfaces, using regular fluorescence microscopy. I can clearly see the fungus using two filter sets: a UV set (ex/em 365/} 420) and a blue filter set (ex/em 470/} 515). While the fluorescence in blue light is much brighter, stuff that is blue/blue-white with UV illumination is green in the blue light. is the "extra" green in the blue light likely to be where the GFP is not as concentrated, and hence doesn't show up in the UV, or is it more likely to be some other compound that has similar excitation/emission characteristics? I have done a fair bit of reading on the subject, but am still feeling a bit vague, and would really appreciate hearing from those of you who have some first hand experience with GFP.
thanks in advance shea
Dr. S.Shea Miller Agriculture & AgriFood Canada Eastern Cereal and Oilseed Research Centre Rm. 2068 Neatby Building Central Experimental Farm Ottawa, Ontario Canada K1A 0C6 Phone: (613) 759-1760 Fax: (613) 759-1701 E-mail: millers-at-em.agr.ca
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Hello everyone; I have just started looking at a GFP-transformed fungus growing on plant surfaces, using regular fluorescence microscopy. I can clearly see the fungus using two filter sets: a UV set (ex/em 365/} 420) and a blue filter set (ex/em 470/} 515). While the fluorescence in blue light is much brighter, stuff that is blue/blue-white with UV illumination is green in the blue light. is the "extra" green in the blue light likely to be where the GFP is not as concentrated, and hence doesn't show up in the UV, or is it more likely to be some other compound that has similar excitation/emission characteristics? I have done a fair bit of reading on the subject, but am still feeling a bit vague, and would really appreciate hearing from those of you who have some first hand experience with GFP.
thanks in advance shea
Dr. S.Shea Miller Agriculture & AgriFood Canada Eastern Cereal and Oilseed Research Centre Rm. 2068 Neatby Building Central Experimental Farm Ottawa, Ontario Canada K1A 0C6 Phone: (613) 759-1760 Fax: (613) 759-1701 E-mail: millers-at-em.agr.ca
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Hi all, We would like to add an individualized computer password system to our FEI/Philips CM-10 TEM. We would like a user to be able to type in their password which would then trigger a switch tied into the high tension and turn the HT on. The system would ideally then have a timer to record "on" time and a function to record this time with the user ID. Connecting a timer to the high tension is not a problem...the computer password part is where we need help. Please contact me if you have a system such as this on a FEI/Philips CM-10 or even on another make TEM which might work on a CM-10. Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
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On Tue, 1 Aug 2000 19:09:28 -0400, Gen Pei wrote:
| ------------------------------------------------------------------------ | The Microscopy ListServer -- Sponsor: The Microscopy Society of America | To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com | On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html | -----------------------------------------------------------------------. | | | Thanks. Gen | ****************************************************************** | Gen Pei | Department of Materials Science and Engineering | Cornell University | 328 Thurston Hall | tele: (607)255-5177 | fax:(607)255-2365 | gp35-at-cornell.edu | | ****************************************************************** |
Nelson Conti 164 Ferne Court Palo Alto, CA 94306
Gen Pei- I've unsubscribed myself one time. I believe that you need to send an e-mail to the MSA Listserver (address = ? ), and just indicate "unsubscribe" in the subject heading. To find the address, go to the MSA Listserver Web address, and it should be possible to find the e-mail address for the MSA Listserver. Sorry, I don't recall it off-hand. Good luck with unsubscribing. Nelson Conti P.S. When in doubt - you can also re-send your message, but direct it to Nestor Zaluzec. I'm sure that he can tell you precisely how to unsubscribe.
_______________________________________________________ Say Bye to Slow Internet! http://www.home.com/xinbox/signup.html
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I have not yet seen any effects, with the Apple Airport system installed for my computers as well as a GigaHertz wireless broadcasting systems which are carrying video signals from one room to another.
The Computer monitors however are a different story.
Nestor
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BOC Gases, has a unique opportunity for an Electron Microscopist/Materials Engineer located at it's state of the art Technical Center in Murray Hill, NJ.
BOC Gases, a division of The BOC Group Inc., was founded in 1888 and is a global leader in the manufacturing and distribution of industrial, medical, and specialty gases and related equipment. Poised for new growth opportunities, BOC currently operates in more than 50 countries, providing customers with gases and technology which are used to support life, freeze food, forge steel, shape glass, refine petroleum, create computer chips, and clean wastewater and smokestack emissions, in addition to hundreds of other applications.
Electron Microscopist/Materials Engineer
Responsibilities
The successful candidate will support the materials characterization needs of the Gases Technology R&D organization using and maintaining analytical equipment, such as, Scanning Electron Microscopy, X-ray Diffraction. Metallography. Characterization includes metallic surfaces, adsorption/catalysis materials as well as other materials related to gases delivery equipment and vessels. This position is within the analytical services part of the Materials Technology Group. More specifically, the person will have the responsibility to:
Provide scanning electron microscope (SEM), environmental scanning electron microscope (ESEM), and metallography services.
Maintain the SEM and ESEM equipment in good operating condition.
Develop new techniques to support researchers needs.
Perform failure analysis and provide technical assistance for material selection projects.
Experience/Education
A B.S. or M.S. in the field of materials science and engineering with practical working experience with scanning electronic microscope techniques. Experience in x-ray diffraction a plus.
A knowledge of a wide variety of materials. Experience with mechanical testing, failure mechanisms, corrosion, and materials, especially metals and polymers. 5-10 years work experience in a materials laboratory.
Work experience in the chemical processing industry a plus.
Ability to perform failure analysis.
Ability to multitask is essential.
Send application to: BOC Gases, Attn: Recruiting - SEM-NM, 575 Mountain Avenue, Murray Hill, NJ 07974. E-mail: jobs-at-us.gases.boc.com
fax: 908-771-1702. Along with opportunities for professional growth we offer competitive compensation and comprehensive benefits.
BOC Gases is an equal opportunity employer.
********************************************************************* This footnote confirms that this e-mail message has been scanned for the presence of known computer viruses by the MessageLabs Virus Control Centre. However, it is still recommended that you use local virus scanning software to monitor for the presence of viruses. *********************************************************************
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Sorry for the previous blank message. Nestor thinks it was just a fluke.
We have an Astromed cooled CCD camera, which we would like to use. However, we have no manuals for it. If you have manuals or other information that would allow us to put this camera into operation please let me know "off line".
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Hi all, We would like to add an individualized computer password system to our FEI/Philips CM-10 TEM. We would like a user to be able to type in their password which would then trigger a switch tied into the high tension and turn the HT on. The system would ideally then have a timer to record "on" time and a function to record this time with the user ID. Connecting a timer to the high tension is not a problem...the computer password part is where we need help. Please contact me if you have a system such as this on a FEI/Philips CM-10 or even on another make TEM which might work on a CM-10. Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
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Sorry for the previous blank message. Nestor thinks it was just a fluke.
We have an Astromed cooled CCD camera, which we would like to use. However, we have no manuals for it. If you have manuals or other information that would allow us to put this camera into operation please let me know "off line".
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Well, I guess Mark and I would have to see a higher magnification image, detailing the polysilicon, the "birds beak" area, and the substrate. This way we could tell whether this is a gate, with gate oxide, or a diffusion contact. I am assuming that your cross section is well into the middle area of the polysilicon line, and not just at the edge.
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When I had resolution problems recently (which turned out to be due to a "leaky" detector) one of the first things my service engineer asked me to check was to make sure that the detector wasn't touching the pole piece (which can cause a ground loop?) Try backing the detector out a little to see if the peak width changes. I doubt it will help, but it is easy to try. Matt
"E. J. McKenzie" {elizm-at-pdx.edu} on 07/28/2000 12:30:59 PM
To: microscopy-at-sparc5.microscopy.com cc:
Hi everyone,
we are having serious problems with the resolution of our EDS detector - the peak height-width ratio is terrible, around 450 counts. Consequently, our peaks look more like rolling hills and one peak blurs into another. I have callibrated the machine using an Al-Cu standard, checked that the detector is kept cool, sample is tilted at 30 degrees, etc etc. I also got another microscopist to come and have a look to check my methods and he also concluded there was a serious problem.
Is there anything else I could try before I phone the Noran technicians and pay them a lot of $$$ to fix/replace the detector?
regards Liz McKenzie -------------------------------------------------- Geomicrobiology and Electron Microscopy Laboratory Room S9 Cramer Hall 1721 SW Broadway Portland State University Portland OR97201
ph:503 725 3362 fax:503 725 3025
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I'm trying to find references for vital staining with fluorobora stains (or fluorabora). There don't seem to be any, so I don't know if this is because they don't work, or because they just didn't catch on. Has anyone any experience of using them, and could anyone give me any references? Thanks.
Lesley Weston.
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Hi , i wanted to know if anyone knew of any SEM/EDS courses in Mexico or Texas. Thanks.
Rafael Peña.
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Well, I guess Mark and I would have to see a higher magnification image, detailing the polysilicon, the "birds beak" area, and the substrate. This way we could tell whether this is a gate, with gate oxide, or a diffusion contact. I am assuming that your cross section is well into the middle area of the polysilicon line, and not just at the edge.
Darrell
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Fellow microscopists,
Have any of you had any experience with the installation of a wireless lan affecting the operation of your microscopes in any way? I would very much appreciate your advice in this regard.
Sincerely,
Mick Thomas ------------------------------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
I am currently being tasked to bring my Amray 3200C SEM into compliance with ISO9000. A major issue with my supervision is the accuracy of the micron bar given with every picture - and what proceedure do I use for callibration. If you have any insight or suggestions, I would appreciate it. Thanks Gary Weaver Gary.Weaver-at-MW.Boeing.com
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Hi all, We would like to add an individualized computer password system to our FEI/Philips CM-10 TEM. We would like a user to be able to type in their password which would then trigger a switch tied into the high tension and turn the HT on. The system would ideally then have a timer to record "on" time and a function to record this time with the user ID. Connecting a timer to the high tension is not a problem...the computer password part is where we need help. Please contact me if you have a system such as this on a FEI/Philips CM-10 or even on another make TEM which might work on a CM-10. Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
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Hello everyone; I have just started looking at a GFP-transformed fungus growing on plant surfaces, using regular fluorescence microscopy. I can clearly see the fungus using two filter sets: a UV set (ex/em 365/} 420) and a blue filter set (ex/em 470/} 515). While the fluorescence in blue light is much brighter, stuff that is blue/blue-white with UV illumination is green in the blue light. is the "extra" green in the blue light likely to be where the GFP is not as concentrated, and hence doesn't show up in the UV, or is it more likely to be some other compound that has similar excitation/emission characteristics? I have done a fair bit of reading on the subject, but am still feeling a bit vague, and would really appreciate hearing from those of you who have some first hand experience with GFP.
thanks in advance shea
Dr. S.Shea Miller Agriculture & AgriFood Canada Eastern Cereal and Oilseed Research Centre Rm. 2068 Neatby Building Central Experimental Farm Ottawa, Ontario Canada K1A 0C6 Phone: (613) 759-1760 Fax: (613) 759-1701 E-mail: millers-at-em.agr.ca
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Date sent: Wed, 02 Aug 2000 11:20:54 -0400 } From: "Shea Miller" {millers-at-em.agr.ca} To: confocal-at-listserv.acsu.buffalo.edu, microscopy-at-sparc5.microscopy.com
I'm trying to find references for vital staining with fluorobora stains (or fluorabora). There don't seem to be any, so I don't know if this is because they don't work, or because they just didn't catch on. Has anyone any experience of using them, and could anyone give me any references? Thanks.
You will still need Nikon Part# 82014 (Optical Coupler 28mm to C-Mount) available only from your local Nikon Microscope Dealer.
Regards,
Lawrence Kordon Nikon, Inc. nikon-at-jagunet.com
"Purdy, Sam" wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Jonathon: } } Contact Diagnostic Instruments, www.diaginc.com or info-at-diaginc.com. } They manufacture couplings to connect cameras and microscopes. } } Sam Purdy } Staff Specialist } National Steel Corp.
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With regard to the posting I made on this subject, Chuck Garber sent me the following note wherein he raises a number of important points. Chuck is correct in all regards. The product we were using was not DURO. We have no experience with Duro. We have made specific, brand name, recommendations in the past and my note was to share our recent experiences regarding these products. The products we were using were not "garage level" products.
So, IF, and only IF, you are using a product solely because of a recommendation from our lab, we now make new recommendations.
Ron
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
Hi Ron,
I guess I should give some kind of disclaimer statement first, that in no way am I suggesting that what you are saying it not correct. If this was your perceived experience, then I am sure it is correct and I am not questioning it.
And it is a fact that there are some garage level operations where people take from 5 gallon pales and "fill" the 1 oz plastic bottles and put on a label and put it into distribution, without any real testing of the integrity of their packaging or any of the other things you and I might think one should be doing to insure the integrity of their product.
But I can assure you, without hesitation, the cyanoacrylate that is made by Duro, which is indeed a consumer products company, but it is also "top in class" when it comes to this type of product, has been offered by us (e.g. SPI) for years and we have never ever had anyone every voice even the slight complaint about it. We also decided a long time ago that once opened, the shelf life of these products can be very short, and consequently, we decided to offer the product in the smaller 3g package.
I go through the trouble to point this out to you because I see things from a slightly different perspective possibly than you do:
1] For one thing, someone even at IBM, having to place an order with either of the two mentioned companies, given the cost of placing an order period of any size, what is the overhead going to be? But if someone was ordering the Duro product from someone like SPI, then it just gets added in with a bunch of other things and the real cost is almost nothing.
2] For someone in Mexico, the situation is far worse. You have a lot of influence on people because of your reputation. Your posting could make someone in Mexico City think that in order to get acceptable results, they have to place a direct order in the USA for one of these "special" cyanoacrylates and in situations with limited research budgets, it just seems to me like they have better ways to be spending their money.
So while the Duro product might not be any better than the two mentioned, the point is that because of the distribution and transportation costs, if people are led to believe that they really do need something that can not be procured from their traditional sources of purchasing, they end up wasting a lot of money unnecessarily.
Of course if you had any hard evidence that the Duro product was in some way inferior, that would surely be another story. But chemically, these are all the same identical materials, they all have the same identical MSDS information and the all perform identically. When ever I have heard of someone having problems it has been because they were using product from a previously opened package.
Feel free to use this information however you feel might be approriate, either in whole or in part. But I did want to share my views with you on your posting, which I hope you won't mind my having done.......
Chuck
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
IBM Analytical Services; http://www.chips.ibm.co
From root Thu Aug 3 23:41:22 2000 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id QAA17937 for dist-Microscopy; Thu, 3 Aug 2000 16:37:42 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id QAA17907 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Thu, 3 Aug 2000 16:37:09 -0500 (CDT)
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With regard to the posting I made on this subject, Chuck Garber sent me the following note wherein he raises a number of important points. Chuck is correct in all regards. The product we were using was not DURO. We have no experience with Duro. We have made specific, brand name, recommendations in the past and my note was to share our recent experiences regarding these products. The products we were using were not "garage level" products.
So, IF, and only IF, you are using a product solely because of a recommendation from our lab, we now make new recommendations.
Ron
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
Hi Ron,
I guess I should give some kind of disclaimer statement first, that in no way am I suggesting that what you are saying it not correct. If this was your perceived experience, then I am sure it is correct and I am not questioning it.
And it is a fact that there are some garage level operations where people take from 5 gallon pales and "fill" the 1 oz plastic bottles and put on a label and put it into distribution, without any real testing of the integrity of their packaging or any of the other things you and I might think one should be doing to insure the integrity of their product.
But I can assure you, without hesitation, the cyanoacrylate that is made by Duro, which is indeed a consumer products company, but it is also "top in class" when it comes to this type of product, has been offered by us (e.g. SPI) for years and we have never ever had anyone every voice even the slight complaint about it. We also decided a long time ago that once opened, the shelf life of these products can be very short, and consequently, we decided to offer the product in the smaller 3g package.
I go through the trouble to point this out to you because I see things from a slightly different perspective possibly than you do:
1] For one thing, someone even at IBM, having to place an order with either of the two mentioned companies, given the cost of placing an order period of any size, what is the overhead going to be? But if someone was ordering the Duro product from someone like SPI, then it just gets added in with a bunch of other things and the real cost is almost nothing.
2] For someone in Mexico, the situation is far worse. You have a lot of influence on people because of your reputation. Your posting could make someone in Mexico City think that in order to get acceptable results, they have to place a direct order in the USA for one of these "special" cyanoacrylates and in situations with limited research budgets, it just seems to me like they have better ways to be spending their money.
So while the Duro product might not be any better than the two mentioned, the point is that because of the distribution and transportation costs, if people are led to believe that they really do need something that can not be procured from their traditional sources of purchasing, they end up wasting a lot of money unnecessarily.
Of course if you had any hard evidence that the Duro product was in some way inferior, that would surely be another story. But chemically, these are all the same identical materials, they all have the same identical MSDS information and the all perform identically. When ever I have heard of someone having problems it has been because they were using product from a previously opened package.
Feel free to use this information however you feel might be approriate, either in whole or in part. But I did want to share my views with you on your posting, which I hope you won't mind my having done.......
Chuck
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
I have been trying to study the ultra-structure (TEM) of strach granules (in wheat and maize seeds) that contains amorphus and crystaline layers. Unfortunately, I am unable to look at these layers under TEM even after keeping the tissues longer time in fixative (over night in 3%gluteraldehyde) and embedding medium (LR white/Spurs for 2 days).
I also tried the the above procedure with isolated starch granules in low melted agar but the result is the same - poor penetration of embedding resin as a result I am getting broken starch granules without any ultrastructural details.
I would appreciate it if anybody could suggest me how to obtaine a reasonably good ultra-structural (TEM) details of starch granules.
With thanks,
Siddique
Get Your Private, Free E-mail from MSN Hotmail at {http://www.hotmail.com/} http://www.hotmail.com
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Below is the program for the Facility Management session at M&M 2000. I am trying to record the sessions so we can summarize as much of the discussion as possible and make it available through the list for those of you who cannot attend. For those of you that can attend, please bring any informationin the form of handouts that you feel helpful in discussion of the topics. I would also appreciate this information sent to me as an attachment so that it can be added to the final summation of the session. Hope to see many of you in Philadelphia. Debby Sherman
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
--------
Experts Session on Facility Management
Wednesday, Aug. 16 9:00AM to Noon Room 106
The following topics will each be briefly introduced by a presenter followed by an open discussion period.
*9:00am: Multi-user Facilities…Managing Users Keith Darling, Ph.D. Manager, Analytical Research Dept, Atofina Chemicals, Inc.
*9:45am: Justification of costs/cost recovery….Electronic bookkeeping and billing. Gregory W. Erdos, Ph.D. Ass’t Director, U. of Florida Biotechnology Program Scientific Director, Electron Microscopy Core Lab
Break
*10:40am: Equipment Maintenance: Vender service contracts vs. Independent providers vs. Insurance company/self-insure options Paul J. Wirtz President, Expense Reduction Analysts International
*Times approximate…Additional discussion starting at 11:30am.
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id KAA03087 for dist-Microscopy; Fri, 4 Aug 2000 10:58:32 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id KAA03084 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 4 Aug 2000 10:58:02 -0500 (CDT) Received: from mail.psych.uic.edu (mail.psych.uic.edu [128.248.41.50]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id KAA03076 for {microscopy-at-msa.microscopy.com} ; Fri, 4 Aug 2000 10:57:20 -0500 (CDT) Received: by mail.psych.uic.edu with Internet Mail Service (5.5.2650.21) id {QGD8M3N5} ; Fri, 4 Aug 2000 10:53:54 -0500 Message-ID: {B98B03BD7E44D411ACE300D0B773E8CA442456-at-mail.psych.uic.edu} "'Dick Burry'" {burry.1-at-osu.edu} , "'Don Theyken'" {dtheyken-at-mindspring.com} , "'Dr. A. J. Tousimis'" {trc-at-tousimis.com} , "'Dr. Ed Daniels'" {ewdaniels-at-earthlink.net} , "'Dr. Hector Caruncho'" {cmhector-at-usc.es} , "'Dr. Hsi-Yuan Yang'" {hyhy-at-ccms.ntu.edu.tw} , "'Dr. Jaci Sagen'" {jsagen-at-miamiproject.med.miami.edu} , "'Dr. Marietta Issidorides'" {mrissid-at-compulink.gr} , "'Dr. P. Chien'" {p-chien-at-nwu.edu} , "'Dr. Sookja Chung'" {skchung-at-hkucc.hku.hk} , "'John Kriho'" {jk-at-meadowsinfo.com} , "'John Robinson'" {robinson.21-at-osu.edu} , "'Kathy Wolken'" {wolken.1-at-osu.edu} , "'Laura Kriho'" {lkriho-at-ucar.edu} , "'Lily Yang'" {lsyang77-at-cm1.hinet.net} , "'Lisa Adler'" {horizonco-at-mindspring.com} , "'List Server'" {microscopy-at-sparc5.microscopy.com} , "'Murat Elcin'" {elcin-at-science.ankara.edu.tr} , "'Nicholas Kriho'" {idklaus1-at-uic.edu} , "'Ralph J. Kriho'" {rjkriho-at-hotmail.com}
} ---------- } From: Hasemeier, Cynthia } Sent: Friday, August 04, 2000 10:39 AM } To: beth bourland; Fran Layne; Ginny; Harry; JDB; Judy Sobiesk; Kathleen } McMullan; marksr; mikedeb; Pauline; Ron; Ted; Fitzgibbon, Genevieve; } Madison, Sybil; Manning, George; Montgomery, John; Kriho, Virginia; Mason, } Sally; Peterson, Jim; Rodriguez, Walter } Subject: Birdcage } } Subject: THE BIRDCAGE } } There once was a man named George Thomas, a pastor in a small New England } town. One Easter Sunday morning he came to the Church carrying a rusty, } bent, old birdcage, and set it by the pulpit. Several eyebrows were raised } and, as if in response, Pastor Thomas began to speak. "I was walking } through town yesterday when I saw a young boy coming toward me swinging } this bird cage. On the bottom of the cage were three little wild birds, } shivering with cold and fright. } } I stopped the lad and asked, 'What you got there son?' } } "Just some old birds," came the reply. } } "What are you gonna do with them?" I asked. } } "Take 'em home and have fun with 'em," he answered. I'm gonna tease 'em } and pull out their feathers to make 'em fight. I'm gonna have a real good } time." } } "But you'll get tired of those birds sooner or later. What will you do?" } } "Oh,! I got some cats," said the little boy. "They like birds. I'll take } 'em to them." } } The pastor was silent for a moment. "How much do you want for those birds, } son?" } } "Huh?!!! Why, you don't want them birds, mister. They're just plain old } field birds. They don't sing - they ain't even pretty!" } } "How much?" the pastor asked again. } } The boy sized up the pastor as if he were crazy and said, "$10?" The } pastor reached in his pocket and took out a ten-dollar bill. He placed it } in the boy's hand. In a flash, the boy was gone. } } The pastor picked up the cage and gently carried it to the end of the } alley where there was a tree and a grassy spot. Setting the cage down, he } opened the door, and by softly tapping the bars persuaded the birds out, } setting them free. Well, that explained the empty birdcage on the pulpit, } and then the pastor began to tell this story* } } One day Satan and Jesus were having a conversation. Satan had just come } from the Garden of Eden, and he was gloating and boasting. "Yes, sir, I } just caught the world full of people down there. Set me a trap, used bait } I knew they couldn't resist. Got 'em all!" } } "What are you going to do with them?" Jesus asked. } } Satan replied, "Oh, I'm gonna have fun! I'm gonna teach them how to marry } and divorce each other, how to hate and abuse each other, how to drink and } smoke and curse. I'm gonna teach them how to invent guns and bombs and } kill each other. I'm really gonna have fun!" } } "And what will you do when you get done with them?" Jesus asked. } } "Oh, I'll kill 'em," Satan glared proudly. } } "How much do you want for them?" Jesus asked. } } "Oh, you don't want those people. They ain't no good. Why, you'll take } them and they'll just hate you. They'll spit on you, curse you and kill } you! You don't want those people!!" } } "How much?" He asked again. } } Satan looked at Jesus and sneered, "All your tears, and all your blood." } } Jesus said, "DONE!" Then He paid the price. } } The pastor picked up the cage he opened the door and he walked from the } pulpit. Isn't it funny how simple it is for people to trash God and then } wonder why the world's going to hell. Isn't it funny how someone can say } "I believe in God" but still follow Satan (who, by the way, also } "believes" in God). } } Isn't it funny how you can send a thousand jokes through e-mail and they } spread like wildfire, but when you start sending messages regarding the } Lord, people think twice about sharing. Isn't it funny how when you go to } forward this message, you will not send it to many on your } address list because you're not sure what they believe, or what they will } think of you for sending it to them. Isn't it funny how I can be more } worried about what other people think of me than what God thinks of me? } } I pray, for everyone who sends this to their entire address book, they } will be blessed by God in a way special for them. } } } } } } } ------------------------------------------------------------ } Cindy Hasemeier, M.A. } IRB Coordinator, Department of Psychiatry } University of Illinois at Chicago } PI 328W, M/C 912 } 312.413.4563 } 312.413.4544 (fax) } }
I know Nestor wants to keep the gratuitous solicitations to a minimum, but VLSI Standards might have a valid, NIST Traceable, ISO compliant solution for you. If need be, I also have some contacts at Amray (KLA-Tencor) that might be able to assist you. Please feel free to contact me directly offline.
Regards -
Marc Helvey VLSI Standards, Inc. 3087 North First Street San Jose, CA 95134-2006 Phone: (408) 428-1800, ext. 108 FAX: (408) 428-9555 Mobile: (408) 307-3833 E-Mail: marc.helvey-at-vlsistd.com {mailto:marc.helvey-at-vlsistd.com} Internet: http://www.vlsistd.com
-----Original Message----- } From: Weaver, Gary E [mailto:Gary.Weaver-at-MW.Boeing.com] Sent: Thursday, August 03, 2000 4:29 PM To: Microscopy-at-sparc5.microscopy.com
I am currently being tasked to bring my Amray 3200C SEM into compliance with ISO9000. A major issue with my supervision is the accuracy of the micron bar given with every picture - and what proceedure do I use for callibration. If you have any insight or suggestions, I would appreciate it. Thanks Gary Weaver Gary.Weaver-at-MW.Boeing.com
Again, thanks to all who have contributed ideas about this IC cross section. I think that I have it sorted out. Maybe 95% right (??). The following URL shows an annotated cross section image. The poly gate is in the central portion of the image above the substrate. I was not sure if lettering would fit or show up.
http://photoweb.net/icxsect3.htm
I have not been able to confirm or deny that the gate truly is poly. My new x-ray system won't be available for several months. It should be a Rontec UHV 133eV system. Some responses indicated that x-ray would work in analyzing the cross sectioned gate. Other responses said it would not. I guess that the proof is in the trying. I suppose that there is some limit in how small the gate is where x-ray will not work. This would be due to either confusion of the results due to x-ray dispersion in a small volume and/or limitations of spot size.
Again, thanks for the great responses. Stay tuned as more info is gained. Further work is getting under way for 0.6u EEPROM analysis. This construction uses TiW plugs.
Dear Listserver members, Sending the Bird Cage to the listserver was a mistake. I thought I was sending to a different listserver. I apologize to all. I will never do this again, but I still cannot believe the furor. Once again I apologize to all. VirginiaKriho
I picked up a big old Zeiss microscope. Care to help me understand what I have? Please send hints in private e-mail and I'll summarize later. My description is at:
I picked up a big old Zeiss microscope. Care to help me understand what I have? Please send hints in private e-mail and I'll summarize later. My description is at:
Your suggested process flow is a little different from all I am used to. First, what etches has this cross section seen? I would venture a guess that the gate is polysilicon, the layer that forms around the poly gate is a CVD (chemical vapor [or is that vapour] deposition) oxide. The next layer is a softer glass deposited and then re-flowed (or spun on). The next layer (marked poly Si), I would guess to be aluminum. The next layer (marked Al), I would guess to be CVD oxide, and the layer on top of that, (marked PSG) might be oxide or silicon nitride.
Mind you, these are all just guesses. In my small world, I have never seen aluminum deposited directly on polysilicon. (That does not mean that it is never done) Aluminum usually shows some texture in a decorated cross section. It is not usually a smooth surface. That level has the appearance I am accustomed to seeing on oxide layers (both gray tones and surface). Most processes define well regions, grow the field oxide, grow the gate oxide, put on the gates, implant source/drains (self aligned to the gates), and then build up from there. The interlevel dielectrics must be deposited, they cannot be grown, like the gate oxide and the field oxide.
I looked through my books, and found a couple with basic process descriptions:
Physical Design of CMOS Integrated Circuits using L-EDIT by John Uymura ISBN 0-534-94326-8 PWS Publishing Co. 20 Park Plaza Boston, MA 02116-4324
Microelectronic Processing An Introduction to the Manufacture of Integrated Circuits by W. Scot Ruska ISBN 0-07-054280-5 McGraw-Hill Book Company
[No financial connections with these books. I have taken several continuing education classes with John, and we have become friends from this long association]
This was a very good parable. Bill Armor, Laverne, OK ----- Original Message ----- } From: Kriho, Virginia {Vkriho-at-psych.uic.edu} To: 'Charles Meshul' {meshulc-at-ohsu.edu} ; 'Dave Yeomans' {yeomans-at-uic.edu} ; 'Dick Burry' {burry.1-at-osu.edu} ; 'Don Theyken' {dtheyken-at-mindspring.com} ; 'Dr. A. J. Tousimis' {trc-at-tousimis.com} ; 'Dr. Ed Daniels' {ewdaniels-at-earthlink.net} ; 'Dr. Hector Caruncho' {cmhector-at-usc.es} ; 'Dr. Hsi-Yuan Yang' {hyhy-at-ccms.ntu.edu.tw} ; 'Dr. Jaci Sagen' {jsagen-at-miamiproject.med.miami.edu} ; 'Dr. Marietta Issidorides' {mrissid-at-compulink.gr} ; 'Dr. P. Chien' {p-chien-at-nwu.edu} ; 'Dr. Sookja Chung' {skchung-at-hkucc.hku.hk} ; 'John Kriho' {jk-at-meadowsinfo.com} ; 'John Robinson' {robinson.21-at-osu.edu} ; 'Kathy Wolken' {wolken.1-at-osu.edu} ; 'Laura Kriho' {lkriho-at-ucar.edu} ; 'Lily Yang' {lsyang77-at-cm1.hinet.net} ; 'Lisa Adler' {horizonco-at-mindspring.com} ; 'List Server' {microscopy-at-sparc5.microscopy.com} ; 'Murat Elcin' {elcin-at-science.ankara.edu.tr} ; 'Nicholas Kriho' {idklaus1-at-uic.edu} ; 'Ralph J. Kriho' {rjkriho-at-hotmail. Sent: Friday, August 04, 2000 10:53 AM
Suprised some one from Polaroid or one of their distributors has not responded to this.
} I've always been told that the brown goop that is easy to get on your hands } while pulling apart the photo & negative of the Polaroid T55 film is a known } carcinogen and to wash your hands immediately upon contact.
According to Polaroid's MSDS (http://www.polaroid.com/service/msds/index.html) the developer in the pods of T55 is an "alkali".
If you look on page 4 of the MSDS you will see the following:
"Does not contain any chemicals listed as carcinogens by OSHA, IARC or NTP"
Guess you'll have to believe them or not.
I've gone to T53, will see how it compares.
The opinions expressed above are my own, blah blah blah yackity smackity.
michael shaffer wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Our roughing pump which service our JSM6300 has began making a } rumbling noise which I might believe is bearings ... altho it could be } the pump's bearings or the motor's. Has anyone serviced this problem? } } tia and cheerios, =shAf= :o) } } {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} } Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu } Geological Science's Electron Probe Facility - University of Oregon } http://epmalab.uoregon.edu/ shAf, I once got a Welsh 1402 for free that was making a terrible knocking noise (also belt drive). When I rebuilt it , I found a alightly swollen phenolic vane that was sticking. A little sanding and a gasket kit and it was as good as new. An off-the-wall problem, but a possibility if the JEOL pump has other than metal vanes.
Ken Converse Quality Images third party SEM service Delta, PA
ELECTRON MICROSCOPIST Laboratory of Structural Biology Institute of Molecular Agrobiology, Singapore
A position is available for an experienced EM technician at the Institute of Molecular Agrobiology (IMA), Singapore, in the group of Dr. Terje Dokland, Laboratory of Structural Biology. The successful candidate will be involved in several research projects, in two broad areas: (1) Structural characterization of viruses by cryo-EM and 3D reconstruction; (2) Study of virus assembly processes in vivo through freeze-substitution, thin sectioning and immuno-cytochemistry. Experience with one or more of these techniques would be advantageous. IMA is a well funded and rapidly expanding research institute which was established in 1995 to conduct basic and applied research in agrobiology. It is located in a modern, spacious and well-equipped building at the National University of Singapore campus. The EM lab presently has a 100kV TEM equipped with a Gatan cryo-stage and a cryo-SEM and a new 200kV LaB6 TEM dedicated to structural microscopy will be purchased shortly. Further information about the institute can be found at http://www.ima.org.sg. Singapore is a cosmopolitan and multicultural society offering all the conveniences of a modern city, and is centrally located in Southeast Asia with easy access to other countries in the region. The position will be filled at the Research Officer or Assistant Research Officer level, depending on experience, and the contract will be initially offered for two years. IMA offers attractive salaries with bonus packages and benefits. Informal enquiries are welcome and can be directed to Terje Dokland at dokland-at-ima.org.sg. Applications, including a CV and names of two referees, should be sent to Terje Dokland Institute of Molecular Agrobiology 1 Research Link The National University of Singapore Singapore 117604
------------------------------------ Terje Dokland Senior Scientist Institute of Molecular Agrobiology 1 Research Link The National University of Singapore Singapore 117604 Phone: 65-872 7405 Fax: 65-872 7007 E-mail: dokland-at-ima.org.sg
"Convictions are greater enemies of truth than lies" F. Nietzsche
I will be attending M&M2000 and I am looking to visit labs and research centers that are utilizing SEM, ESEM and AFM in their studies in the fields of oil industry such as oil well cement, catalyst, corrosion and concrete. Any names, addresses will be highly appreciated. Thanks Mansour Al-Shafei Senior Lab Sci. Saudi Aramco Oil Company Lab Research & Development Center Analytical Sciences Division Advanced Instruments Unit Voice: 966-3-876-4360 E-mail: shafeima-at-aramco.com.sa
I have been asked to help a colleague with a problem he is having cutting good sections for light microscopy. He has a Reichert-Jung 2030 microtome and a new tungsten carbide microtome knife (I do not know the exact type of knife). He is trying to cut ABS with a R scale value of 105 and polycarbonate with a M scale value of 70 (R-value of ~120 according to him).
Every section he has made exhibits numerous scratch marks that are also evident in the block face. I briefly tried to face the block and also got scratch marks. I cleaned the knife edge, tried different cutting angles and speeds (manual only). I have cut this material to make good sections and block face but I used an ultramicrotome and diamond knife. But to do this for all his work is not an option.
Any suggestions as to how the sections and the block face can be improved?
Thanks so much in advance,
Damian Neuberger, Ph.D. Baxter Healthcare, Inc. WG3-2S P.O. Box 490 Round Lake, IL 60073 e-mail: damian_neuberger-at-baxter.co, Tel: 847-270-5888 Fax: 847-270-5897
Does anyone know why freshly made lead citrate sometimes comes out yellow, and what adverse effects, if any, result? In going over past threads on the subject, it seems most people have a relatively colorless product as the end result, but a few report the yellow color as normal. After years of making up "colorless" lead citrate, mine is now yellow. I've toyed with NaOH sources and pH; I use ultra pure water, I've cleaned glasswear 'till it squeaks...no change....it's still yellow.
Sections seem to stain okay, although the stain goes bad very quickly, sometimes even on the grid itself. Since this can be a problem with colorless lead citrate, I'm not sure the yellowing is the cause. I'd sure appreciate any feedback!
Thanks, Margaret -- Margaret Dienelt Plant Pathologist
Electron Microscopy Lab FNPRU, National Arboretum ARS, USDA
B. 010A R. 238 BARC-W 10300 Baltimore Avenue Beltsville, MD. 20705
I agree with Darrell 100%. He has identified all the layers as I see them. The aluminum always show some grain boundaries after staining the section with a BOE as mentioned that you have used on these sections. Also, the aluminum would not be deposited on top of the polysilicon.
Mark
} ---------- } From: "milesd-at-us.ibm.com"-at-sparc5.microscopy.com } Sent: Friday, August 4, 2000 3:50 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: Re: IC Structure } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Gary, } } Your suggested process flow is a little different from all I am used to. } First, what etches has this cross section seen? I would venture a } guess that the gate is polysilicon, the layer that forms around the poly } gate is a CVD (chemical vapor [or is that vapour] deposition) oxide. } The next layer is a softer glass deposited and then re-flowed (or } spun on). The next layer (marked poly Si), I would guess to be } aluminum. The next layer (marked Al), I would guess to be CVD } oxide, and the layer on top of that, (marked PSG) might be oxide or } silicon nitride. } } Mind you, these are all just guesses. In my small world, I have never } seen aluminum deposited directly on polysilicon. (That does not } mean that it is never done) Aluminum usually shows some texture in } a decorated cross section. It is not usually a smooth surface. That } level has the appearance I am accustomed to seeing on oxide } layers (both gray tones and surface). Most processes define well } regions, grow the field oxide, grow the gate oxide, put on the gates, } implant source/drains (self aligned to the gates), and then build up } from there. The interlevel dielectrics must be deposited, they cannot } be grown, like the gate oxide and the field oxide. } } I looked through my books, and found a couple with basic process } descriptions: } } Physical Design of CMOS Integrated Circuits } using L-EDIT } by John Uymura } ISBN 0-534-94326-8 } PWS Publishing Co. } 20 Park Plaza } Boston, MA 02116-4324 } } Microelectronic Processing } An Introduction to the Manufacture of Integrated } Circuits } by W. Scot Ruska } ISBN 0-07-054280-5 } McGraw-Hill Book Company } } [No financial connections with these books. I have taken } several continuing education classes with John, and we } have become friends from this long association] } } Darrell }
Specimen Preparation Engineer Northwestern University
The Electron Probe Instrumentation Center (EPIC) at Northwestern University has an immediate opening for a specimen preparation expert. EPIC is a part of the world renowned Materials Research Center (MRC) and the Department of Materials Science & Engineering at Northwestern. The EPIC facility serves over 120 users in all aspects of Scanning and Transmission Electron Microscopy. The role of the specimen preparation engineer is to assist users with their specimen preparation needs, including instruction in TEM and SEM sample preparation using IBT, FIB, PIPS, electropolishing, ultramicrotomy, cutting/grinding/polishing, vacuum evaporation etc. All microscopes in EPIC are under full service contract. Thus, the duties include training students/users, development of specialized techniques and applications, minor maintenance, record keeping and billing. A BS or technical degree in physical/biological sciences is required. The candidate must have hands-on experience in all aspects of specimen preparation as well as considerable familiarity with digital acquisition, processing and computer assisted techniques. All levels of experience will be considered. Compensation will be commensurate with experience and qualifications. Send cover letter, resume and three references to: Prof. Vinayak P. Dravid, Director EPIC Materials Science & Engineering Northwestern University, 3013A MLSB Evanston, IL 60208 E-mail: v-dravid-at-northwestern.edu Fax: (847) 491-7820 http://epic.ms.nwu.edu/epic/index.htm
Northwestern University is an Affirmative Action/Equal Opportunity Employer. Hiring is contingent upon eligibility to work in the United States.
I thank Jan for supplying the following information.
-----Original Message----- } From: Ringnalda, Jan [mailto:JRingnalda-at-FEICO.com] Sent: Monday, August 07, 2000 9:12 AM To: 'Ingber, Bruce F.'; Long, Jo; Piscopo, Irene Cc: Schaub, Daniel; Ringnalda, Jan; Rice, Trisha (CS); Booth, Steve; 'sherman-at-btny.purdue.edu'
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Folks, I requested information on a computer log-in system a week ago and received a number of replys. I am pursuing some of them including the program written by Tom Ickes which is not available at the moment but may become available soon. Also, I did install the freeware: CM Lock from FEI and it does work great as a means to control access to the TEM. However, it does not record users and use time for later billing. I am still investigating how we can record the log-ins for this purpose. What I did receive is below:
------------------------ FEI Co. (www.feico.com) has on their web site number of useful freeware software for Philips CM microscopes. One of them is "CM lock". The program runs on Win box and does what you asked for. Give them a try. I use is on my CM300 to define different levels of privileges for my users and disable microscope controls for off time.
Jerzy Gazda, Ph.D.
---------------
We have a rather good system here that does what you want and quite a lot more. It will handle several machines. It keeps track of account numbers and summaries billing information. The system was developed by Tom Ickes who was an employee here at the time. He has since left Lehigh but may be interested in setting up a system for you (for a fee, I imagine). The system is well designed in that it uses off the shelf parts, is controlled by a PC and has software that has been very well constructed.
His e-mail is: tomickes-at-cswebmail.com
Good luck, Alwyn Eades
---------------------
Debby,
I might suggest that you add a digital I/O card that will supply analog outputs from the computer used to run the TEM. These outputs could then be used for a type of interlock for turning on the HT. I would not suggest switching the HT itself. Once this was in place, with software like Visual Basic or National Instruments LabWindows or LabView you can write a routine to control the HT, mark time, and write user info to a spread sheet. I might suggest you contact National Instruments Http://www.natinst.com as they can supply all you need for this project. On another thought, if your controlling computer is a PC and can run Windows NT the password issue is much easier since it has controlled log on features built in. Not a specific answer but hope this is useful to you.
Roy Beavers
--------------
Dear Debby,
I have recently contacted Richard Benassi at BEI in Maryland. He makes the exact control mechanisms/software you are talking about. I think he's a bit pricey ($1,000 for 2 control boxes w/software), but that's just my opinion...
I too am looking to control & monitor microscopes, so if you come across something else would you be so kind as to pass the information on to me?
Thanks and good luck,
Jim
--------
If I come up with additional information, I'll send it on. Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id LAA09944 for dist-Microscopy; Mon, 7 Aug 2000 11:44:50 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id LAA09940 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Mon, 7 Aug 2000 11:44:20 -0500 (CDT) Received: from lulu.it.northwestern.edu (lulu.acns.nwu.edu [129.105.16.54]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id LAA09928 for {Microscopy-at-sparc5.microscopy.com} ; Mon, 7 Aug 2000 11:44:09 -0500 (CDT) Received: (from mailnull-at-localhost) by lulu.it.northwestern.edu (8.8.7/8.8.7) id LAA01115 for {Microscopy-at-sparc5.microscopy.com} ; Mon, 7 Aug 2000 11:38:30 -0500 (CDT) Received: from paiute (paiute.ms.nwu.edu [129.105.37.230]) by lulu.acns.nwu.edu via smap (V2.0) id xma000203; Mon, 7 Aug 00 11:36:49 -0500 Message-ID: {007701c0008d$95991440$e6256981-at-ms.northwestern.edu}
Specimen Preparation Engineer Northwestern University
The Electron Probe Instrumentation Center (EPIC) at Northwestern University has an immediate opening for a specimen preparation expert. EPIC is a part of the world renowned Materials Research Center (MRC) and the Department of Materials Science & Engineering at Northwestern. The EPIC facility serves over 120 users in all aspects of Scanning and Transmission Electron Microscopy. The role of the specimen preparation engineer is to assist users with their specimen preparation needs, including instruction in TEM and SEM sample preparation using IBT, FIB, PIPS, electropolishing, ultramicrotomy, cutting/grinding/polishing, vacuum evaporation etc. All microscopes in EPIC are under full service contract. Thus, the duties include training students/users, development of specialized techniques and applications, minor maintenance, record keeping and billing. A BS or technical degree in physical/biological sciences is required. The candidate must have hands-on experience in all aspects of specimen preparation as well as considerable familiarity with digital acquisition, processing and computer assisted techniques. All levels of experience will be considered. Compensation will be commensurate with experience and qualifications. Send cover letter, resume and three references to: Prof. Vinayak P. Dravid, Director EPIC Materials Science & Engineering Northwestern University, 3013A MLSB Evanston, IL 60208 E-mail: v-dravid-at-northwestern.edu Fax: (847) 491-7820 http://epic.ms.nwu.edu/epic/index.htm
Northwestern University is an Affirmative Action/Equal Opportunity Employer. Hiring is contingent upon eligibility to work in the United States.
Hi All, I was wondering if anyone had experience or comments on how much oxygen can safely be pumped before one has to opt for fomblin type oil in rotary pumps. Does the same limit apply to turbo pump oil, and do the pumps need to be completely cleaned for the changeover? I believe I've heard that it is unwise to try to clean a pump and reuse it, with the change of oil? Also, are there any less expensive sources for oils that can be safely used when pumping oxygen. Any comments, suggestions, or hints would be welcome.
Many Thanks,
Steve Buckingham Excellatron Solid State (770) 438 2201 All the Best,
Steve. Kratos Analytical Inc. (770) 251 6490 "Fill your bowl to the brim, and it will spill. Keep sharpening your knife, and it will blunt. Chase after money and security, and your heart will never unclench. Care about people's approval, and you will be their prisoner. Do your work, then step back... The only path to serenity." Lao-tze. Tao te Ching
Does anyone use TEM electron diffraction patterns for identifying silica in soil samples? I know that X-ray diffraction is a suitable technique, but I have been asked to find an alternative method such as TEM. If this is possible what would the sample prep entail?
Thanks in advance
Jean Howard
________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
RMC was purchased by Ventana Medical Systems, 1-800-356-3452 or 1-800-277-4613ext3023. Greg Carter is the ultramicrotome service manager. I had him visit my lab to service our MT2B in December, 1999. Let me know if this does not work.
Sincerely, Ken
On Mon, 7 Aug 2000, Geoff Williams wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I was just informed by our Purchasing department that their RMC contact } #s no longer work. } } Is there anyone out there that can point me to the direction of the } company that might or might not still be RMC that can provide parts for } our MT2B? } } Please excuse me if this answer is common knowledge, I just can't seem } to find the information. } } TIA, } Geoff Williams } } Electron Microscope Facility Supervisor } Biology Department } Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } } Geoffrey.Lloyd.Williams-at-cmich.edu } 517 774-3576 } 517 774-3462 (fax) } } } }
You might also try getting your MT2B (I thought I was the only one still using one!) from Bill McGee at Microtome Services Company - 315-451-1404. He is a great guy to deal with, very reasonably priced, and has provided me with excellent service for a long time.
} } } Margaret Brannigan {brannign-at-asrr.arsusda.gov} 08/07/00 14:59 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi,
Does anyone know why freshly made lead citrate sometimes comes out yellow, and what adverse effects, if any, result? In going over past threads on the subject, it seems most people have a relatively colorless product as the end result, but a few report the yellow color as normal. After years of making up "colorless" lead citrate, mine is now yellow. I've toyed with NaOH sources and pH; I use ultra pure water, I've cleaned glasswear 'till it squeaks...no change....it's still yellow.
Sections seem to stain okay, although the stain goes bad very quickly, sometimes even on the grid itself. Since this can be a problem with colorless lead citrate, I'm not sure the yellowing is the cause. I'd sure appreciate any feedback!
Thanks, Margaret -- Margaret Dienelt Plant Pathologist
Electron Microscopy Lab FNPRU, National Arboretum ARS, USDA
B. 010A R. 238 BARC-W 10300 Baltimore Avenue Beltsville, MD. 20705
Telephone: (301) 504-6097 Fax: (301) 504-5096
Margaret,
Lead citrate turning yellow is a new one on me. I've never heard of that peoblem. When I make lead citrate, I use a 100ml bottle that previously was used for HBSS ( I find they work better than a volumetric flask, easier to use), put the bottle on a magnetic sirrer, add lead citrate, close the bottle, let it stir for a while then had concentrated NaOH ( Fisher catalog # ss277) let it stir until solution clears, put it in refrigerator overnight (keeping it stored in refrig. at all times until use). Never turned yellow on me. I've made up my stain this way for almost 30 years and it never turned yellow and I've been able to use the stain for several months. If you have any questions, call me.
Phil Rutledge USDA/ARS voice: 410 788-4136 or 2120 e-mail:prutledge-at-ars.usda.gov fax: 410 788-4399
Yes, Although we do no do with soil sample we do identify particles from different kinds of oxides on the metal surface. With support of holly carbon film electron diffraction can be obtained from silica if the size of silica particles is around, say 100 nm or less. We have obtained electron pattern from extracted silica.
yours Sincerelly
Ping Liu
Listers:
Does anyone use TEM electron diffraction patterns for identifying silica in soil samples? I know that X-ray diffraction is a suitable technique, but I have been asked to find an alternative method such as TEM. If this is possible what would the sample prep entail?
I have a constant problem of regularly spaced wrinkles on my Spurr sections on the TEM. I stain with uranyl acetate in 50% ethanol for about 30 minutes followed by stable lead for 15 minutes.(Wrinkling is even worse with methanolic UA). I am certain the problem has something to do with staining since few wrinkles appear when I observe unstained sections. I also rarely encounter wrinkled sections with Embed 812 sections when using aqueous UA stains. Any suggestions?
} Does anyone use TEM electron diffraction patterns for identifying silica in } soil samples? I know that X-ray diffraction is a suitable technique, but I } have been asked to find an alternative method such as TEM. If this is } possible what would the sample prep entail? }
Dear Jean, I have taken selected-area ED (SAED) patterns of minerals in soil samples, but I have not yet attempted to get a structure. Of course, identi- fying a silicate of known structure from one or more zone-axis patterns is a simpler problem. The sample preparation is very easy--I just suspend the soil in water, dilute to the appropriate degree so that the soil particles
are well dispersed, put a few microliters on a carbon-formvar grid, and air-dry. When you find a particle of interest, you may have to orient the grid to get a zone axis, so you need a double-tilt or tilt-rotation stage. Once you have the proper orientation, you can ascertain the crystal form and measure the unit cell parameters and intensities. If you have an image simulation program, you can calculate the predicted intensities from a trial structure (which matches the unit cell parameters) and see what kind of agreement you get. This should work even if there is some dynamical scattering. You could try an ab initio structure determination if the cry- stal is thin enough and the voltage high enough so that dynamical scat- tering is not significant, but this is much more difficult and requires much more data. See Doug Dorset's book, Structural Electron Crystal- lography for a good overview. Good luck. Yours, Bill Tivol
Thanks for the positive feed-back and the chance for discussion.
1. If you notice the figure is labeled seed. This seed is a co-polymer of BA-B. The reason for the difference in shadow length even after cross linking with OsO4 is the heterogeneous composition. I think this is due to the polymerization rates of the two monomers. Butadiene polymerizes faster than BA so it will form particles first and the BA follows. This was then to be the seed in a core-shell latex. If you look at the third figure on the web page you will see the final latex.
2. You are right, at just the right orientation the core should look centered, but the chance of that happening frequently is small. However, if you look at the group of three particle around 11:00 you will see the middle particle having a fairly centered core.
If you have any other questions please e-mail me. Discussion is good.
Olga Shaffer
"Gerroir, Paul J" wrote:
} Hi Olga, } An impressive start to your web page! The polymer chemists/engineers here } will find these images very exciting. } I have two comments/questions. } 1. In your first example of TEM - Staining you have illustrated that a "very } short shadow indicating very little PB" It is not clear in this example of } shadowing and staining whether the system, PB/PBA is a copolymer or as I } suspect the seed referred to is PBA and the shadowing gives an indication of } the amount of shell, i.e. PB associated with the seed or core. } 2. In your second example of TEM - Staining you have pointed out the } off-center nature of the PS core. If we think of these images in two } dimensions and the latex spheres being randomly oriented why don't we see } any particles where the core appears to be more centered? If you were to } view these two-dimensional spheres from the left or right in your images } wouldn't a picture of a more centralized core result? Make a simple } three-dimensional model and try this experiment, then perhaps you can offer } me an explanation. Do you think it a little puzzling that these particles } all orient similarly? } } Regards, } Paul } } Paul J. Gerroir } Microscopy } Materials Characterization } Xerox Research Centre of Canada } 2660 Speakman Drive } Mississauga, Ontario L5K 2L1 } } Phone: (905) 823-7091, ext. 216 } FAX: (905) 822-7022 } e-mail: paul.gerroir-at-crt.xerox.com
Yes TEM electron diffraction patterns for identifying silica in soil samples can be done. One quick way is to sonicate some of the sample in water maybe with some detergent added (do different dilutions because what looks like a little bit can be a lot in the TEM.) Then take an aliquot .5-1ul and drop it onto a carbon film/holey grid. (we used to collapse our own MCE filters after lightly coating them with carbon) Allow the grid to dry, then place in scope. We used to start with the most dilute prep first and would get results off it.
good luck
} Listers:
} Does anyone use TEM electron diffraction patterns for identifying } silica in soil samples? I know that X-ray diffraction is a suitable } technique, but I have been asked to find an alternative method } such as TEM. If this is possible what would the sample prep } entail?
} Thanks in advance
} Jean Howard Jon Ekman Associate Research Specialist University of Wisconsin Milwaukee 414-229-6471
Dear Jean, I've done ED studies of soils from time to time. I have not done the sample preparation myself, but it's not too difficult. You need to disperse the soil in a liquid such as water or 2-propanol and let a drop of the suspension dry on a normal TEM grid. An ultrasonic bath is ideal for this. If the silica grains are substantially bigger than the clays, there may be fractionation, resulting in a reduced yield of silica as it can fall to the bottom of the suspension. Care is therefore needed in sampling. If the silica grains are big enough (one micron or more) you can use a double-tilt or rotation-tilt holder to align the crystallites to get a sensible zone pattern which will make interpretation easier. If the grains are v. small and you need to use a micro-diffraction technique you may find the tilting process drives you crazy as it's v. difficult to keep the grain in the beam during tilting.
Thr camera length of the microscope varies with specimen height, and specimen height varies with tilting in duoble-tilt holders. Generally, external calibration (e.g. camera length vs objective current) is not wholly satisfactory but in your case may be adequate. The projector astigmatism needs to be very well corrected if accurate d-spacings are to be obtained. A sure-fire way round this is to use an internal standard such as a poly- crystalline Al or Au film evaporated onto the carbon support film. This produces a ring pattern superimposed on the spot pattern, allowing the actual camera length in any direction to be measured directly. (Modern sputter coaters produce Au films where the grain size is too small to give sharp rings, so it has to be evaporation from a hot W wire.)
Not all silica in soils is crystalline, though, and my preferred method is to do TEM-EDX, which can resolve very small areas as the limiting factor is the minimum probe size that still gives a reasonable number of counts. It's also a lot quicker and easier.
Regards, Eric
---------------------- Dr Eric Lachowski Department of Chemistry University of Aberdeen Meston Walk Old Aberdeen AB24 3UE Scotland +44 (0)1224 272934 fax 272921 e.lachowski-at-abdn.ac.uk
A sophisticated method of soil sample analysis by high resolution TEM is thin sectioning. That requires complete sample dehydration in 100 % EtOH, gradual infiltration with LR White, (representative) suspension sampling followed by embedding in gel capsules, curing, and thin sectioning on a microtome. I recommend to collect sections on Cu / carbon lacey grids. No need to osmicate or post-stain unless you're interested in soil organisms.
Alice Dohnalkova Environmental Microbiology Battelle, PNNL MS P7-50 Richland, WA 99352 tel. (509) 372-0692 fax (509) 376-1321
-----Original Message----- } From: Jean Howard [SMTP:jmhowrd-at-hotmail.com] Sent: Monday, August 07, 2000 8:46 PM To: Microscopy-at-sparc5.microscopy.com
Listers:
Does anyone use TEM electron diffraction patterns for identifying silica in soil samples? I know that X-ray diffraction is a suitable technique, but I have been asked to find an alternative method such as TEM. If this is possible what would the sample prep entail?
Thanks in advance
Jean Howard
________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
Excuse me folks, but isnt everyone putting the carriage before the horse? Dont you think that as scientists you need to be a bit less eager and a bit more conscientious to tell Jean Howard yes when there are more important questions to be answered?
Jean; why do you have to find an alternative method? Do you not have a diffractometer and a competent XRD person on staff? If not, why bother getting into a can of worms? Are you to analyze the soils for the regulatory crystalline silica content of 0.1% by weight for hazmat, product labelling? Why analyze it by a method that is not accepted? XRD is the accepted method for analyzing air samples for the respirable fraction by NIOSH. Almost all competent scientists that I know of analyze bulk materials with several modifications depending on the sample.
If you go and do it by TEM, you could be putting yourself in a dangerous liability situation causing question of your capabilities as a scientist. Doing it by TEM is frought with problems, and how do you propose to accurately and precisely quantify it at the 0.1% by weight level?.......you cant do it, at least not reproducibly.
The XRD method is the only way to do it if you want as close to real numbers as you can get. There are plenty of labs that will analyze it wrong for $50 a pop by XRD. I know.....I have been doing good analyses for 10 yrs, and it takes a hell of a lot more than $50 worth of work. You need to find out what the sample is composed of first, then remove components gravimetrically, with acid dissolutions or thermal treatments to render phyllosilicates amorphous. Most micas and clays have interfering peaks at the primary, secondary and tertiary quartz peaks. How do you know you arent analyzing beta or quasi metastable polymorphs of tridymite and cristobalite? You dont.
I can recommend some top notch people to verify what I have said, and who can do it by XRD better than most. Let me know. I am disappointed at how quickly so many people are responding to say it can be done by TEM without really thinking about the problems that need to be addressed first. Good way to chop your reputation up.
Lou Solebello
----- Original Message ----- } From: Ping Liu {ping.liu-at-sandvik.com} To: {Microscopy-at-sparc5.microscopy.com} Sent: Tuesday, August 08, 2000 5:50 AM
Fellow Microscopists,
I am looking for 2 reliable, "clean" polyclonal (preferred) or monoclonal antibodies for use with aldehyde/detergent-treated or ethanol-treated cultured cells (HeLa). One antibody would be against vimentin, and the other against a protein that allows clear visualization of mitochondria. Any suggestions? Thank you in advance. Regards,
Andrew Ochalski, Microscopy Technician, Dept. of Biology, University of Ottawa Room 108, Gendron Bldg. 130 Louis Pasteur, Ottawa, Ontario CANADA K1N 6N5 613-562-5800 x6343 FAX: 613-562 5486
Have you tried aqueous stains for the Spurr's? I've used aq. UA/Pb and had nice staining. If you have some stain-resistant plant material, you can use KMnO4 instead of UA - it stains well.
Tamara Howard CSHL
On Tue, 8 Aug 2000, Anthony Greco wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have a constant problem of regularly spaced wrinkles on my Spurr } sections on the TEM. I stain with uranyl acetate in 50% ethanol for } about 30 minutes followed by stable lead for 15 minutes.(Wrinkling is } even worse with methanolic UA). I am certain the problem has something } to do with staining since few wrinkles appear when I observe unstained } sections. I also rarely encounter wrinkled sections with Embed 812 } sections when using aqueous UA stains. Any suggestions? } } Tony Greco } } }
Hello all, Does anybody have any experience in getting 50 kV Power Supplies for Amray 1800 series electron microscopes fixed. The power supply was manufactured by CPS, a company now out of Oregon. Approximately two weeks ago we fedex'd them our supply for repair (which cost an arm and a leg), and after all this time, they're still can't even give me an approximate date when it will be completed. They haven't even diagnosed it yet. Obviously we're dead in the water with out it, and work is piling up. Does anybody know of any other options. Possibly other shops that can repair these supplies, or possibly another source for a different supply. I'm so frustrated at the fact that they didn't look at it for a week, and the fact that they have no clue when it will be done. I of all people can understand being busy, but I can't live another two weeks with the e-scope down for the count. If anybody has any ideas that would help to restore my sanity, or get my supply fixed, I would be forever indebted.
Thanks in advance, ~Jonathan Dunlap
Jonathan Dunlap Analytical Laboratory Manager Osram Sylvania Inc. 816 Lexington Avenue Warren, PA 16365 Ph: 814-726-6991 Fax: 814-726-6956
I have an aging (~15years old) Balzers Critical Point Dryer that has a burnt out heater. The dryer is type 11120 B no. 337. The heater is a plastic strip 20 cm long and 3.5cm wide which seems to have carbon or graphite laminated into the strip and connecting wires are soldered onto each end. This wraps around the chamber of the unit and was held there by heat shrink wrap. Does anyone know if you can get replacements for this heater ( in Australia would help!)? I am waiting to hear from Balzers but need to get it fixed quickly.
Regards Dave
Dave Phelan EM/X-Ray Unit University of Newcastle NSW 2308 AUSTRALIA Ph 02 4921 5667 Fax 02 4921 7019 emudp-at-mail.newcastle.edu.au
I have worked on their 30KV units in my Amray scopes. The problem is either the driver transistor or the HV generator block.
I have the schematics for the 30KV CPS unit. The HV block was about $250. The transistor was $7 from Radio Shack. I zapped the unit when the stub made hard contact with the pole piece in my 1600T. The CPS units in my 1830 and 1910 have never failed.
I found CPS to be quite helpful. I guess that this may have changed.
gary g.
At 03:25 PM 8/8/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
First of all, let me clarify what is being requested. The lab is interested in the regulatory crystalline silica content in soils. Currently the samples are being contracted out for XRD because there is no diffractomer or XRD scientist in house. Their results are reliable and NIOSH acceptable.
The question was whether the TEM (which is in lab) could be used for this analysis instead of purchasing a diffractometer. Let me say that I have no experience with minerals by TEM. My work has been primarily with polished metal samples. I had some doubts about using TEM for this type of work since I was not successful in finding literature on such.
I appreciate all the responses and apologize for not being more specific. No one's competency is in question here. I have received numerous suggestions but it is clear to me that the most reliable method is XRD. Should I need to speak to someone further I will not hesitate to contact you Lou.
Thank you all
Jean Howard ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
Provided you can obtain a binary or thresholded grain boundary image, there are a number of general image analysis programs available - free of charge - that will automatically extract the grain size. Try: * "ImageJ" http://rsb.info.nih.gov/ij/index.html * "Scion Image" http://www.scioncorp.com/frames/fr_scion_products.htm * "Image Tool" http://macorb.uthscsa.edu/dig/itdesc.html
Cheers, Paul Baggethun
} ---------- } From: Maria Luiza[SMTP:luiza-at-ppgem.faenquil.br] } Sent: Monday, July 31, 2000 7:31 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: Grain size....... } } Hi. I am a studant from Brazil and I´m trying to measure the grain size of } my samples (They are Niobium 9%Tantalun). } I know there are a lot of softwares available on the internet, but I don´t } know where and if there is a specific program to analise the grain size of } metals. } Please, remember that I work at Brazil, so I don´t have any money buy a } software. We allways try to do the best we can. } } Thank you very much..... João Paulo Barros Machado..... } } Please answer this email for: jpbm-at-easygold.com.br } } }
If you are making a last minute decision as to which conferences to attend this summer/fall then take a look at ICHC 2000 "Cell Biology and Imaging Tools for the New Century". 3-8th Sept. 2000 York, UK
Take look at the fantastic Final Programme at http://www.med.ic.ac.uk/external/ichc_2000
Over 100 leading speakers in 35 symposia
York is a most beautiful medieval walled city, a great place for a vacation. You can register for the week or if close to York then come for the day!
Hello everyone; now that the corn has finally started to do something in this miserable excuse for a summer we are having, we have a grad student who would like to do some peroxidase histochemistry on a rather daunting number of samples and varieties. is there any fixation he can use to preserve both his samples and his enzyme activity? he will not be able to look at everything in a reasonable length of time. thanks, as always, in advance shea
Dr. S.Shea Miller Agriculture & AgriFood Canada Eastern Cereal and Oilseed Research Centre Rm. 2068 Neatby Building Central Experimental Farm Ottawa, Ontario Canada K1A 0C6 Phone: (613) 759-1760 Fax: (613) 759-1701 E-mail: millers-at-em.agr.ca
I have a student that would like to do TEM of diamond films that are prepared by microwave plasma CVD. The thickness of the films can be adjusted to make it electron beam transparent. The student believes that he can flake off pieces of the film.
I'm thinking maybe we could just lay the diamond film on a grid and pop it into the TEM. Is this a crazy idea? Does anyone have any suggestions?
} Excuse me folks, but isnt everyone putting the carriage before the horse? } Dont you think that as scientists you need to be a bit less eager and a bit } more conscientious to tell Jean Howard yes when there are more important } questions to be answered? }
I guess that those of us who answered thought of the question from an academic/technical viewpoint--true, at least, in my case. Yes, if there are legal questions about the methods, that is a consideration I did not think about.
} } Jean; why do you have to find an alternative method? Do you not have a } diffractometer and a competent XRD person on staff? If not, why bother } getting into a can of worms?
To characterize tens to hundreds of individual soil particles that are in the micrometer size range can be done in a few (long) sessions on the TEM. This includes EDS, ED, and imaging. I don't think that the same info can be obtained in as short a time by XRD.
} Are you to analyze the soils for the regulatory } crystalline silica content of 0.1% by weight for hazmat, product labelling?
I hadn't considered this aspect.
} } Why analyze it by a method that is not accepted? XRD is the accepted } method for analyzing air samples for the respirable fraction by NIOSH. } Almost all competent scientists that I know of analyze bulk materials with } several modifications depending on the sample.
However, to analyse many individual particles and distinguish between, e.g., sodium aluminum silicate and potassium aluminum silicate is not best done by XRD.
} } } If you go and do it by TEM, you could be putting yourself in a dangerous } liability situation causing question of your capabilities as a scientist.
As a scientist, or as a lawyer?
} } Doing it by TEM is frought with problems, and how do you propose to } accurately and precisely quantify it at the 0.1% by weight level?.......you } cant do it, at least not reproducibly.
To get the distribution of various silicates to a precision of 0.1%
would, indeed, require analysis of many thousand (million?) particles. Of course, one could, nonetheless, get such precision given enough time.
} } } The XRD method is the only way to do it if you want as close to real numbers } as you can get. There are plenty of labs that will analyze it wrong for $50 } a pop by XRD. I know.....I have been doing good analyses for 10 yrs, and it } takes a hell of a lot more than $50 worth of work. You need to find out what } the sample is composed of first, then remove components gravimetrically, } with acid dissolutions or thermal treatments to render phyllosilicates } amorphous. Most micas and clays have interfering peaks at the primary, } secondary and tertiary quartz peaks. How do you know you arent analyzing } beta or quasi metastable polymorphs of tridymite and cristobalite? You dont.
Here is where the simplicity of the TEM preparation, ability to take images, and elemental analyses of individual particles can answer these questions more quickly than just using XRD. If there is a mixture of two isomorphous silicates--say one with sodium and one with potassium but otherwise the same--I would not be optimistic about quantitating them to the 0.1% level from the ring pattern intensities, but EDS should do the job easily in a few minutes.
} } I can recommend some top notch people to verify what I have said, and who } can do it by XRD better than most. Let me know. I am disappointed at how } quickly so many people are responding to say it can be done by TEM without } really thinking about the problems that need to be addressed first. Good way } to chop your reputation up.
I look to this list as a forum for the discussion of technical points. As you said, there are often other considerations. I am happy to give technical advice where I am knowledgable and leave the decision of whether the other criteria will be met to someone else. Yours, Bill Tivol
Peroxidase is fairly resistant to glutaraldehyde or Karnovsky's fixatives, so good morphology should be possible. see JL Hall and R Sexton (1972) Cytochemical localization of peroxidase activity in root cells. Planta 108, 103-120.
Date sent: Wed, 09 Aug 2000 10:25:49 -0400 } From: "Shea Miller" {millers-at-em.agr.ca} To: microscopy-at-sparc5.microscopy.com
Off-and-on since the 1970s I've been preparing uranyl acetate for en bloc staining using maleate buffer at pH 5.2 adjusted with NaOH. However, the pKa I for maleic acid is 2.0 and the general rule for buffer selection is that the the desired pH should be within +/- one pH unit of the pKa value (p. 46 in Buffer Solutions,The Basics by Beynon and Easterby; IRL Press, 1996). Therefore, maleate buffer appears not to have a good buffering capacity at pH 5.2. Does anyone understand why maleate was chosen as bufffer for this procedure, and what I may be missing in my thinking on this subject? A current reference for this en bloc staining technique is found in Biomedical Electron Microscopy by Maunsbach and Afzelius, Academic Press, 1999.
/John/
John D. Wright, Ph.D. West Desert Test Center Dugway, UT
RMC is still part of Ventana Medical systems Inc. Feel free to contact me directly for Sales, Applications support & Service issues.
Drop by & see us at M & M
Best,
Al Coritz North American Sales Manager Ventana-RMC Microscopy Products Group 1-800-227-2155 x 2773 Direct: 520-690-2773 Fax: 520-690-2759 Cell: 520-906-3268
-----Original Message----- } From: Ken Tiekotter [mailto:tiekotte-at-up.edu] Sent: Tuesday, August 08, 2000 2:23 AM To: Geoff Williams Cc: Microscopy Listserver
RMC was purchased by Ventana Medical Systems, 1-800-356-3452 or 1-800-277-4613ext3023. Greg Carter is the ultramicrotome service manager. I had him visit my lab to service our MT2B in December, 1999. Let me know if this does not work.
Sincerely, Ken
On Mon, 7 Aug 2000, Geoff Williams wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I was just informed by our Purchasing department that their RMC contact } #s no longer work. } } Is there anyone out there that can point me to the direction of the } company that might or might not still be RMC that can provide parts for } our MT2B? } } Please excuse me if this answer is common knowledge, I just can't seem } to find the information. } } TIA, } Geoff Williams } } Electron Microscope Facility Supervisor } Biology Department } Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } } Geoffrey.Lloyd.Williams-at-cmich.edu } 517 774-3576 } 517 774-3462 (fax) } } } }
The MSA Education Committee is once again organizing the FREE Exhibitor Demonstrations on Tuesday, Aug. 15, 2000 at 5:00 pm. Below is a list of participants and the titles of their tutorial workshops. If you wish to attend one of these, please come to the MSA Education booth (now part of the MSA Mega-Booth) on the Exhibit Hall floor to sign up and receive a ticket allowing you to attend.
This list may not be exhaustive, so come by the booth to see what has been added!
Advanced Microscopy Techniques Corp. - Advantage HR Digital Camera System.
Allied High Tech Products, Inc. - Precision Sample Preparation for TEM, SEM and PreFIB (Multiple Sample) Thinning Using the Multiprep and MicroVision Measurement and Archiving Software.
Digital Instruments, Veeco Metrology Group - Surface Metrology Instrumentation: An Overview of Atomic Force Microscopes, Optical Interference Microscopes and Stylus Profilers.
E.A. Fischione Instruments, Inc. - Contamination-Free Specimen Preparation.
EDAX, Inc. - Quantitative Analysis of EDS Data from the Low-Vacuum SEM.
Electron Microscopy Sciences - Developments in Ultra Small Immunogold Silver Detection Systems.
Evex Analytical - Basic and Intermediate Features of the Evex Microanalysis System.
FEI Company - Precautions for Collecting EDS Data in a Low-Vac SEM.
KS Electron Technologies - Up Close with the Worlds Most Affordable TEM.
LEO Electron Microscopy, Inc. - EFTEM in Practice.
Micro Photonics, Inc. - Non-Destructive 3D Microscopy Using X-Ray Microtomography.
Microbiology/Syncroscopy - Bring Your World Into Focus with Syncroscopy.
Motic Incorporation, Ltd. - All-In-One Digital Microscope with Powerful Software.
NORAN Instruments, Inc. - Phase Identification and Orientation Mapping by EBSD.
Photon Technology International, Inc. - NovaLight: the NEW Power in Microscope Illumination.
SouthBay Technology, Inc. - Electropolishing, Tripod Polishing and Dimpling; Benefits and Limitations.
Ted Pella, Inc. - New Microwave Techniques, Accessories and Equipment.
TSL - #1 Advances in Phase Identification in the SEM. #2 Orientation Mapping in the TEM.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
I have a student who would like to do TEM on samples of clay in polystyrene. The particles are very small (around 1 nm in thickness) and are in suspension are in suspension. He believes that we could scoop up some sample onto a carbon film and examine them in an SEM. He has a chemistry paper where someone did just that. I have a TEM that has a voltage range of 100-200kV. I have never worked on polymers or a clay.
Questions: Will clay or polystyrene contaminate the TEM? What voltage range is safe? Any tricks?
By the way I do have a stage that can be liquid nitrogen cooled to minimize radiation damage.
The plant samples can be fixed in 4% paraformaldehyde and 0.5% GTA in phosphate buffer pH 7.2. I embed in paraffin and make 5-6 micron sections. I normally use the Vector Lab kit (no financial interest), peroxidase or alkaline phosphatase to do my enzyme histochemistry and it works beautifully. You can also use any fluorescent probe to label your protein of interest.
Good luck,
Soumitra ************************************************************* Soumitra Ghoshroy Ph.D. Electron Microscopy Lab Box 3EML New Mexico State University Las Cruces, NM 88003 Tel: 505-646-3600 Fax: 505-646-5665 e-mail:sghoshro-at-nmsu.edu http://confocal.nmsu.edu/eml
On Wed, 9 Aug 2000, Shea Miller wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello everyone; } now that the corn has finally started to do something in this miserable excuse for a summer we are having, we have a grad student who would like to do some peroxidase histochemistry on a rather daunting number of samples and varieties. } is there any fixation he can use to preserve both his samples and his enzyme activity? he will not be able to look at everything in a reasonable length of time. } thanks, as always, in advance } shea } } } } Dr. S.Shea Miller } Agriculture & AgriFood Canada } Eastern Cereal and Oilseed Research Centre } Rm. 2068 Neatby Building } Central Experimental Farm } Ottawa, Ontario } Canada K1A 0C6 } Phone: (613) 759-1760 } Fax: (613) 759-1701 } E-mail: millers-at-em.agr.ca } } }
At 11:18 AM -0600 8/9/00, Wright, John D. wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
****************** Simply put, UA does not ppt in maleate buffer. Phosphates cause hideous problems, and I've been told that cacodylate is also problematic. I side-step the whole issue and do my en bloc UA during dehydration (3%UA in 50% EtOh for 1 hr following an initial short step in 50% EtOh to remove the buffer. It works for me. Does it raise anyone's hackles? If yes, why?
Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Robin, If you put it on NaCl, you can float them off. You might be able to deposit them right onto freshly cleaved mica and float them off. You might also be able to put them onto either SiO or carbon support films directly.
If the films are too thin, they may not pop off your substrate. I had DLC films that were about 0.8 um pop off after a few days because of the stress in them. You might have to cut them down a little to help.
Carbon resists ion milling without some help in the gas. If you are interested in cross section TEM, then you will need to do low angle ion milling. I have a paper in MRS TEM book and the effects of gas compositions on ion milling. You might want to use a combination of Ne and O2.
The small angle cleavage technique worked beautifully on my DLC samples on Si. The DLC was too thin at the edge for EELS work. I would definitely try it with this if you put it on Si, GaAs, sapphire, SiC, or glass.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
} -----Original Message----- } From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com } [mailto:"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com] } Sent: Wednesday, August 09, 2000 11:09 AM } To: microscopy-at-sparc5.microscopy.com } Subject: TEM of diamond films } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html } } } -------------------------------------------------------------- } ---------. } } } I have a student that would like to do TEM of diamond films that are } prepared by microwave plasma CVD. The thickness of the films can be } adjusted to make it electron beam transparent. The student } believes that he } can flake off pieces of the film. } } I'm thinking maybe we could just lay the diamond film on a } grid and pop it } into the TEM. Is this a crazy idea? Does anyone have any } suggestions? } } Thanks, } } Robin Griffin }
I would like to have copies of literature on the Wild M8 stereomicroscope and its accessories. Sales literature, price list, users manual, parts diagrams, service manuals,etc. are all welcome. Please contact me first to avoid duplication of your generous efforts. I will glady reimburse copying and mailing costs.
Mike Dalbey Biology Dept. University of California Santa Cruz, CA 95064
I suspect it would pop straight off if it became charged, i.e almost immediately you put the beam onto it!
One approach is to make a sticky grid, and put the film onto that. Three possible methods - 1) dip the grid into a thin solution of epoxy resin in PPO, allow all solvent to evaporate, place diamond film and cure epoxy at 60oC 2) Dissolve a pressure-sensitive adhesive from your favourite brand of sticky tape or carbon tabs. Dip grid, allow to dry, place film. 3) Coat grid with a thermoplastic solution (e.g. polystyrene) and dry. place diamond film. Bond by melting plastic on a hot-plate.
For a non-adhesive-based method you could try sandwiching the diamond film between two grids stuck together at one edge with epoxy, or use a proprietary sandwich grid (obtainable from SPI, Ted Pella, Polysciences, Agar Scientific etc.) Chris
} From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com To: microscopy-at-sparc5.microscopy.com
somehow you need to glue the diamond film onto the grid, otherwise you might lose the diamond film inside the TEM.
On Wed, 9 Aug 2000 rgriffin-at-eng.uab.edu-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have a student that would like to do TEM of diamond films that are } prepared by microwave plasma CVD. The thickness of the films can be } adjusted to make it electron beam transparent. The student believes that he } can flake off pieces of the film. } } I'm thinking maybe we could just lay the diamond film on a grid and pop it } into the TEM. Is this a crazy idea? Does anyone have any suggestions? } } Thanks, } } Robin Griffin }
I'm interested in calculating pore density using SEM image. I used several free softwares but couldn't get satisfactory results. If someone knows software or methods about calculating pore density using SEM image, please let me know.
It is not clear from your posting if your sample is a powder or a bulk specimen. The 1 nm you mention, I assume is this the clay thickness ?
We have looked at different clay materials in a polymer matrix . We normally tended to section these in a microtome (actually a cryo microtome to minimize deformation of the matrix.). We tend to use 200KeV for most of our work, but 100KeV should be OK also. The key here is to get nice thin sections because the contrast of the very thin clay particles will be better if the sections are thin. Also , could you share with me the paper (reference )you mentioned ? . We don't use a cooling holder for our samples, and contamination has not been a problem for us but then we were not doing diffraction on individual particles, we were only looking at the dispersion of the clay.
There is another possibility also which I would like to try when I get a chance. This would involve etching (plasma?) the surface of the sample to expose some of the clay and then looking at the sample either by AFM or by TEM (replicas of the etched surface). This should work on film or bulk specimens (with the clay in suspension), but I am not sure it would work OK on powder samples.
I hope this helps,
Jordi Marti Honeywell
-----Original Message----- } From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com [mailto:"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com] Sent: Wednesday, August 09, 2000 3:25 PM To: microscopy-at-sparc5.microscopy.com
I have a student who would like to do TEM on samples of clay in polystyrene. The particles are very small (around 1 nm in thickness) and are in suspension are in suspension. He believes that we could scoop up some sample onto a carbon film and examine them in an SEM. He has a chemistry paper where someone did just that. I have a TEM that has a voltage range of 100-200kV. I have never worked on polymers or a clay.
Questions: Will clay or polystyrene contaminate the TEM? What voltage range is safe? Any tricks?
By the way I do have a stage that can be liquid nitrogen cooled to minimize radiation damage.
There will be a special symposium which is dedicated to Dr. Lee Peachey, a well respected scientist and microscopist. The Symposium is number 16A on Tuesday, August 15th in Room 108. The Other Motor city: Muscle and Non-muscle Motility A Dedication to Dr. Lee Peachey
Dr. Lee Peachey uses confocal microscopy and High Voltage EM to study 3-D structural information on cell structures. The line up for the session is terrific. Dr. Saul Winegrad will chair the morning session and Dr. Clara Frazini-Armstrong will chair the afternoon session. All of the presenters are the tops in there fields. One highlight is Sir Andrew Huxley from Trinity College, Cambridge will give a talk on Microscopy of Muscle in the 19th and 20th Centuries. Dr. Huxley received the Nobel Prize in Medicine in 1963 for discoveries concerning the ionic mechanisms involved in excitation and inhibition in the peripheral and contrail portions of the nerve cell membrane.
Don't miss this session, Regards, Andy
Andrea S. Weisberg NIH/NIAID/LVD Bldg. 4/Room 210 4 Center Drive Bethesda, MD 20892-0445 phone: (301) 435-1977 fax: (301) 480-1147 e-mail: aweisberg-at-nih.gov
I wondered if you tried baking the grids and sections in a drying oven at 70 degrees C for 10 minutes or so? This will ensure that the sections will not come off the grids in any way, if that is what is happening here. (since you report that you don't seem to have this problem on unstained sections, it seems to me that your sections might be lifting a bit from the grid, causing wrinkling) If you leave the sections in the oven longer, or even overnight, I've noticed that it doesn't seem to harm them in any way. Once the sections are baked onto the grids in this manner, even a firehose directed at full spray cannot remove the sections. I might also add that heating the sections in this manner does not affect them adversely in staining. It might be the quick fix that you are looking for.
Garry
} ---------- } From: Tamara Howard[SMTP:howard-at-cshl.org] } Sent: Tuesday, August 08, 2000 2:29 PM } To: Anthony Greco } Cc: Microscopy-at-sparc5.microscopy.com } Subject: Re: TEM/Wrinkled Spurr Sections } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Have you tried aqueous stains for the Spurr's? I've used aq. UA/Pb and had } nice staining. If you have some stain-resistant plant material, you can } use KMnO4 instead of UA - it stains well. } } Tamara Howard } CSHL } } } On Tue, 8 Aug 2000, Anthony Greco wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I have a constant problem of regularly spaced wrinkles on my Spurr } } sections on the TEM. I stain with uranyl acetate in 50% ethanol for } } about 30 minutes followed by stable lead for 15 minutes.(Wrinkling is } } even worse with methanolic UA). I am certain the problem has something } } to do with staining since few wrinkles appear when I observe unstained } } sections. I also rarely encounter wrinkled sections with Embed 812 } } sections when using aqueous UA stains. Any suggestions? } } } } Tony Greco } } } } } } } }
New York Microscopical Society 1244 McBride Avenue West Paterson, NJ 07424
Bernard Friedman Memorial Workshop
Use of the Microscope September 16, 23, 30, October 7, 2000
A basic course on light microscopy which will cover the following topics: Theory of microscopy Kohler Illumination Diffraction Theory Contrast Methods Polarized light Phase Contrast Interference . . . . Hoffman contrast, Rheinberg, Dark-field & oblique Illumination, etc.
The workshop will consist of four consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of microscopy. The course instructors include Jan Hinsch of Leica, Inc., Dennis O’Leary of Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of SensIR Technologies, Inc. and N.Y.M.S. Instructor Don O'Leary.
WHEN: September 16, 23,30, October 7, 2000 from 10 A.M. to 4 P.M.
WHERE: 1244 McBride Avenue, West Paterson, NJ. Phone (973) 812-8377 (Free parking, accessible by public transportation, Information on car pools and transportation will be provided.)
COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership) Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: Beginners and experienced users who wish to learn more about the proper use of a microscope.
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PLEASE POST ---------------------------------------------------------------------------- ------------------- Registration Form Use of the Microscope
} At 11:18 AM -0600 8/9/00, Wright, John D. wrote: } } } } } Off-and-on since the 1970s I've been preparing uranyl acetate for en bloc } } staining using maleate buffer at pH 5.2 adjusted with NaOH. However, the } } pKa I for maleic acid is 2.0 and the general rule for buffer selection is } } that the the desired pH should be within +/- one pH unit of the pKa value } } (p. 46 in Buffer Solutions,The Basics by Beynon and Easterby; IRL Press, } } 1996). Therefore, maleate buffer appears not to have a good buffering } } capacity at pH 5.2. Does anyone understand why maleate was chosen as } } bufffer for this procedure, and what I may be missing in my thinking on this } } subject? A current reference for this en bloc staining technique is found } } in Biomedical Electron Microscopy by Maunsbach and Afzelius, Academic Press, } } 1999. } } } } /John/ } } } } John D. Wright, Ph.D. } } West Desert Test Center } } Dugway, UT } } ****************** } Simply put, UA does not ppt in maleate buffer. Phosphates cause hideous } problems, and I've been told that cacodylate is also problematic. } I side-step the whole issue and do my en bloc UA during dehydration (3%UA } in 50% EtOh for 1 hr following an initial short step in 50% EtOh to remove } the buffer. It works for me. Does it raise anyone's hackles? If yes, why? } } Lee } } Leona Cohen-Gould, M.S. } Sr. Staff Associate } Director, Electron Microscopy Core Facility } Manager, Optical Microscopy Core Facility } Joan & Sanford I. Weill Medical College } of Cornell University } voice (212)746-6146 } fax (212)746-8175
Well, my hackels are not up but I have read that long (more than a few hours) exposure to ethanol will extract cytoplasmic material even after glut. and osmium. I do agree that UA will give terrible ppt. with phosphate and I think cacodylate as well. Many rinses are needed to get the salts out. I have read that UA in distilled water works well for en bloc staining. Maleate buffer always seemed like a pain to me.
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
} Does anyone know why freshly made lead citrate sometimes comes out } yellow, and what adverse effects, if any, result? In going over } past threads on the subject, it seems most people have a relatively } colorless product as the end result, but a few report the yellow } color as normal. After years of making up "colorless" lead citrate, } mine is now yellow. I've toyed with NaOH sources and pH; I use } ultra pure water, I've cleaned glasswear 'till it squeaks...no } change....it's still yellow. } } Sections seem to stain okay, although the stain goes bad very } quickly, sometimes even on the grid itself. Since this can be a } problem with colorless lead citrate, I'm not sure the yellowing is } the cause. I'd sure appreciate any feedback! } This happened to me a year ago, and I was equally puzzled. First I thought it was a reaction with the rubber on the plunger of the 10 ml syringe in which I store my stain (with a filter on the end it makes a great drop dispenser). I had opened a new box of *old* syringes. So I changed back to my old *new* syringes, but the newly made stain was still yellow and short lived. Same water, temperture, glassware, balance, etc. as my usual stuff. I suspected the distllled water source.
The next day a woman who has been doing TEM for ca. 35 years came in to ask if I'd ever seen yellow lead citrate. She had just goten this for the first time. She works in a different lab in a diffrent building with different water and, in fact, there should be absolutely no correlation at all. Except for phase of the moon, which theory we briefly entertained.
A few days later I successfully made up clear lead citrate. The main change I had made was to make up fresh 10N NaOH. However, the phase of the moon had changed as well.
Good luck!
Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
vendor-supplied slurry is used in lens polishing. recent polishings have resulted in scratches on lens. i have been asked to provide data with my xl40feg regarding particle sizes. am i correct in assuming that particle size distribution (in the slurry environment) is as important a criteria as simple particle diameters? can i evaluate simple particle size by "boiling off" the suspension, and sprinkling conductive tape with the remaining powder? can i "fire" the slurry into a ceramic brick, and polish the result for slurry particle distribution analysis? what would the procedure entail? thanks.
You could try the website for the American Association of Blood Banks, www.aabb.org. It is a trade organization. They may have technical leads and links.
I'm researching about the microstructure of steel, and have been usually using zet polishing and ion milling. Now I'm considering wedge polisher (Tripod) as a TEM sample preparation method, but rarely know about this.
I'd like to know whether this method can be used generally ,compared with zet polishing and ion milling. And I'd like to know advantages and disadvantages. Also, would you let me know the companies that produce wedge polisher (Tripod)?
Thanks in advace.
JungSoo.
Jung-Soo Byun School of Materials Science & Engineering College of Enineering, Seoul National University Seoul 151-742, Korea Tel : +82-2-880-7100, Fax : +82-2-885-9671 H.P. : 016-226-4358
You might consider filtering the slurry, rinsing the filtrate well. Choice of filter media is important. For you application, I would suggest a membrane filter rather than a fiberous one. ..Then dry, coat, and examine on filter -or apply filtrate to carbon tape.
Many slurrys contain more than just water and abrasive. Lubricants, suspention agents , etc. can be present which may coat the particulate if evaporative techniques are used alone.
As for firing - no. Under the correct (wrong) conditions, you may not just sinter the powder, but grain size and aglomerations may well be affected.
Woody White McDermott Technology Inc.
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vendor-supplied slurry is used in lens polishing. recent polishings have resulted in scratches on lens. i have been asked to provide data with my xl40feg regarding particle sizes. am i correct in assuming that particle size distribution (in the slurry environment) is as important a criteria as simple particle diameters? can i evaluate simple particle size by "boiling off" the suspension, and sprinkling conductive tape with the remaining powder? can i "fire" the slurry into a ceramic brick, and polish the result for slurry particle distribution analysis? what would the procedure entail? thanks.
I dont understand what is meant by x140feg. Is that an SEM? We do slurry analysis on daily basis using a TEM. Sample preparation includes to dilute slurry to about 1%vol and to follow a standard "drop grid" procedure. Please let me know if you have any further questions.
Have a nice day!
Chao Ni, PhD Rodel Inc. 451 Bellevue Road Newark, DE 19713 (302) 366-0500 ext 2812
-----Original Message----- } From: Mark Riggs [mailto:riggsm-at-svg.com] Sent: Thursday, August 10, 2000 6:55 PM To: Microscopy-at-sparc5.microscopy.com
vendor-supplied slurry is used in lens polishing. recent polishings have resulted in scratches on lens. i have been asked to provide data with my xl40feg regarding particle sizes. am i correct in assuming that particle size distribution (in the slurry environment) is as important a criteria as simple particle diameters? can i evaluate simple particle size by "boiling off" the suspension, and sprinkling conductive tape with the remaining powder? can i "fire" the slurry into a ceramic brick, and polish the result for slurry particle distribution analysis? what would the procedure entail? thanks.
Our old coater is on it's last legs so I'm looking to purchase a new carbon coater for our FESEM for EDS work. Since I inherited the old coater 'm looking for any advice (and good or bad experiences) with carbon coaters from the various manufacturers. The unit should be a bench top unit, user friendly, and as automatic as possible since it will be operated by several users.
Thanks in advance, Ron Kneisler Albemarle Corporation ron_kneisler-at-albemarle.com ****************************************************************************************** IMPORTANT NOTICE: This email message and any files transmitted with it may be confidential, may be legally privileged, and are for the intended recipient only. If obtained in error, please delete this message and confirm the deletion in an email to the sender. ******************************************************************************************
Hello everyone; after lurking for months, I have my second question in a week. Thanks to everyone who replied to my question re: fixation for enzyme histochemistry... we have decided to go with a short fixation in 1.75% glutaraldedhyde in buffer, and are keeping our fingers crossed (Kristin... I will reply in more detail to you).
Another grad student is wanting to do some in situ hybridizations on soybean and soybean seed coat. She would like to know if you can use lyophilized material.... I have only used stuff that was harvested fresh, fixed and processed through paraplast using a normal solvent dehydration, so I cannot advise her here. Any advice would be most helpful.
thanks in advance shea
Dr. S.Shea Miller Agriculture & AgriFood Canada Eastern Cereal and Oilseed Research Centre Rm. 2068 Neatby Building Central Experimental Farm Ottawa, Ontario Canada K1A 0C6 Phone: (613) 759-1760 Fax: (613) 759-1701 E-mail: millers-at-em.agr.ca
Would anyone have the web site of the "Sociedade Brasileira de Microscopia e Microanalise - SBMM"? Thanks in advance,
Adriana
Adriana Pinheiro Martinelli Rodriguez, PhD Laboratorio de Biotecnologia Vegetal CENA, Universidade de Sao Paulo Av. Centenário 303, Cx. Postal 96 13400-970, Piracicaba, SP, Brasil phone: +55-19- 429-4694 fax: +55-19- 429-4610 adriana-at-cena.usp.br http://www.cena.usp.br/labs/labbiotecveg.htm
I am trying to help a Hartford, CT high school biology teacher find microscopes for her classes. Any leads, ideas, advice for locating microscopes? I'm in Connecticut but welcome input from anywhere.
Julie Gross Dept. of Neuroscience University of CT Health Center Farmington Ave. Farmington, CT 06030 jgross-at-neuron.uchc.edu
You should look at the ProjectMicro Pages on the MSA WWW Site. There is a lot of information contained therein.
http://www.msa.microscopy.com/ProjectMicro
Caroline Schooley is the best contact for additional details/help. Her contact info is on the above page.
Nestor Your Friendly Neighborhood SysOp
} X-Sender: jgross-at-neuron.uchc.edu } Date: Fri, 11 Aug 2000 10:55:00 -0400 } To: MSA Listserver {Microscopy-at-sparc5.microscopy.com} } From: Julie Gross {jgross-at-neuron.uchc.edu} } Subject: Microscopes for High School } Mime-Version: 1.0 } Status: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
================================================================== Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov ================================================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ==================================================================
The box said "This program requires Win 95/98/NT or better..." so I bought a G3 Mac
Bruce F. Ingber, Biologist USDA-ARS, SRRC 1100 Robert E. Lee Blvd. New Orleans, LA 70124 (504) 286-4270 phone (504) 286-4419 fax bingber-at-commserver.srrc.usda.gov
-----Original Message----- } From: Ringnalda, Jan [mailto:JRingnalda-at-FEICO.com] Sent: Friday, August 11, 2000 9:47 AM To: 'Ingber, Bruce F.'; Long, Jo; Piscopo, Irene Cc: Schaub, Daniel; Rice, Trisha (CS); Booth, Steve; 'sherman-at-btny.purdue.edu'
Ron,
We have a Bal-Tec SCD 050 Coater (6+ yrs. old) with two separate heads for carbon sputtering and gold/palladium sputtering respectively. We use carbon threads for sputtering. It has been very satisfactory for our FESEM work. The Bal-Tec unit is quite user-friendly.
------------------------------------------------------------ } Our old coater is on it's last legs so I'm looking to purchase a new carbon } coater for our FESEM for EDS work. Since I inherited the old coater 'm looking } for any advice (and good or bad experiences) with carbon coaters from the } various manufacturers. The unit should be a bench top unit, user friendly, and } as automatic as possible since it will be operated by several users. } } Thanks in advance, } Ron Kneisler } Albemarle Corporation } ron_kneisler-at-albemarle.com
As you are doing FESEM work, have you considered a high resolution deposition system? Our IBS/e Ion Beam Sputter Deposition and Etching System can act as a carbon "coater", but it can also deposit chromium, iridium etc. Using ion beam deposition allows you to controllably deposit very thin, extremely uniform films without heating the sample. Just a thought. Let me know if you would like additional information.
DISCLAIMER: South Bay Technology produces equipment and supplies as described above and, therefore, has a vested interest in promoting their use.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by INTERNET:"Ron_Kneisler-at-albemarle.com"-at-sparc5.microscopy.com } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Our old coater is on it's last legs so I'm looking to purchase a new carbon coater for our FESEM for EDS work. Since I inherited the old coater 'm looking for any advice (and good or bad experiences) with carbon coaters from the various manufacturers. The unit should be a bench top unit, user friendly, and as automatic as possible since it will be operated by several users.
Thanks in advance, Ron Kneisler Albemarle Corporation ron_kneisler-at-albemarle.com {
South Bay Technology, Inc. is the manufacturer of the Tripod Polisher which is based on original work performed at the IBM East Fishkill facility (Anderson, Klepeis, Benedict et al). The Tripod Polisher, using the wedge polishing technique, can be used for a variety of materials. It has been used successfully for thinning semiconductors from silicon to indium phosphide, metals, ceramics, geological samples, hard disk media, composites etc. When used properly, some materials can be mechanically polished to electron transparency without the need for ion milling. Typically, a short ion milling step is required.
Jet polishing, in general, is a great method for preparing homogeneous materials if you do not have a specific area of interest. Jet polishing is a quick, effective and damage free method of preparing specimens. Of course, with a single jet polisher, you can control the area within the sample that you want to reach, but even at that, cannot very easily pick a specific spot. The Tripod Polisher provides a means to thin to a very specific area - in the case of semiconductors even to a specific sub-micron device. The main advantage of the Tripod Polisher is control. Tripod Polishing is an iterative process of polishing and visually inspecting your sample. Certain parts of the process can be automated - such as the first side polish (essentially an SEM cross section) by simply placing the Tripod on the yoke of a polishing machine. This is a nice way to do it as you can easily lift the polisher off for visual inspection and replace it within seconds. If alignment is done properly prior to 1st side polishing, first side visual inspection is minimal. The second side polish is more critical as that is where you are trying to locate your specific area of interest and progress the wedge in such a way as to maximize the thin area. Ongoing visual inspection is critical on the 2nd side or "wedge" polishing step. 2nd side polishing is generally done by hand ( although Allied High Tech sells a system that they say automates the whole process) as this allows you the most control and is the quickest way to change between the polishing and visual inspection.
The polishing is done using diamond abrasive films in a series of steps. The amount of time that you spend on any one polishing step is very short so it is important that you can quickly remove the Tripod Polisher and the diamond film to move on to the next step. This is important for a few reasons, but one of the most important is that the material you are removing at these final steps is so small, that you can over polish very quickly if you are not careful.
We have been doing Tripod Polishing here for over 10 years and have a huge base of customers as well as an extensive library of technical papers and application notes. Also, we do conduct ongoing courses in TEM specimen preparation including a specialized course in Tripod Polishing. I would be pleased to send you more detailed information if you have an interest. I hope this helps.
DISCLAIMER: South Bay Technology produces equipment and supplies as described above and, therefore, has a vested interest in promoting their use.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by "=?euc-kr?B?uq/BpLz2?=" } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi,
I'm researching about the microstructure of steel, and have been usually using zet polishing and ion milling. Now I'm considering wedge polisher (Tripod) as a TEM sample preparation method, but rarely know about this.
I'd like to know whether this method can be used generally ,compared with zet polishing and ion milling. And I'd like to know advantages and disadvantages. Also, would you let me know the companies that produce wedge polisher (Tripod)?
Thanks in advace.
JungSoo.
Jung-Soo Byun School of Materials Science & Engineering College of Enineering, Seoul National University Seoul 151-742, Korea Tel : +82-2-880-7100, Fax : +82-2-885-9671 H.P. : 016-226-4358
We need a nuclear LM stain for tissue sections (3-4 microns thickness) which have been processed with DAB label and embedded in epoxy. Does anyone have such a method? We would much appreciate any ideas.
We have received many questions concerning the use of Mercox for corrosion casting recently, but to provide first hand experience to those people and any others who may have an interest, we are once again sponsering the Vascular Corrosion Casting symposium at the M&M meeting next week in Philadelhia. This symposium will once again be chaired by Dr. Hossler, Dr. Aharinejad, and Dr. Lametschwandtner. It will be held Wed., Aug 16 at 8:30 am in room C122. I would urge all those who are interested to attend.
John Arnott Chairman --
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net
uranyl acetate results in giving precipitates in our hands with either PBS or sodium cacodylate. For cell cultures we do the following: After osmium we do several washes with water and do the en bloc staining with 1% UA in water for 1h in the dark. We usally make a 4% UA solution and check the pH with paper before use. It should be between 4 and 5. If not we discard the solution.
Christoph Bauer University of Chicago
"Wright, John D." wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Off-and-on since the 1970s I've been preparing uranyl acetate for en bloc } staining using maleate buffer at pH 5.2 adjusted with NaOH. However, the } pKa I for maleic acid is 2.0 and the general rule for buffer selection is } that the the desired pH should be within +/- one pH unit of the pKa value } (p. 46 in Buffer Solutions,The Basics by Beynon and Easterby; IRL Press, } 1996). Therefore, maleate buffer appears not to have a good buffering } capacity at pH 5.2. Does anyone understand why maleate was chosen as } bufffer for this procedure, and what I may be missing in my thinking on this } subject? A current reference for this en bloc staining technique is found } in Biomedical Electron Microscopy by Maunsbach and Afzelius, Academic Press, } 1999. } } /John/ } } John D. Wright, Ph.D. } West Desert Test Center } Dugway, UT
If your substrate is brittle, you can also cleave it to make a small slab that you glue on a TEM grid. If the substrate is too thick, you may need to pre-thin it by mechanical polishing.
Then look with the TEM along the cleaved edge at about 45° tilt.
It's fast, it's clean, it does not require any complex tools, but of course the the transparent area is quite small!
Best regards
Philippe Buffat
} } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I have a student that would like to do TEM of diamond films that are } } prepared by microwave plasma CVD. The thickness of the films can be } } adjusted to make it electron beam transparent. The student } believes that he } } can flake off pieces of the film. } } } } I'm thinking maybe we could just lay the diamond film on a grid and pop it } } into the TEM. Is this a crazy idea? Does anyone have any suggestions? } } } } Thanks, } } } } Robin Griffin } } } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Dr Chris Jeffree } University of Edinburgh } Biological Sciences EM Facility } Daniel Rutherford Building } King's Buildings EDINBURGH EH9 3JH } Tel: +44 (0) 131 650 5345 } FAX: +44 (0) 131 650 6563 } } Inveresk Cottage, 26 Carberry Road, } Inveresk, Musselburgh, Midlothian EH21 8PR, UK } Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401 } FAX: +44 (0) 131 653 6248 } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
__________________________________________________________________ Philippe Buffat Ecole Polytechnique Federale de Lausanne (EPFL) Centre Interdepartemental de Microscopie Electronique Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time) E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/ ______________________________ Eudora F2.1 ___________________________
} } On Mon, 7 Aug 2000, Margaret Brannigan wrote: } } } Does anyone know why freshly made lead citrate sometimes comes out } } yellow, and what adverse effects, if any, result? } } } } Sections seem to stain okay, although the stain goes bad very } } quickly, sometimes even on the grid itself. Since this can be a } } problem with colorless lead citrate, I'm not sure the yellowing is } } the cause. I'd sure appreciate any feedback! } }
[skip]
} } A few days later I successfully made up clear lead citrate. The main } change I had made was to make up fresh 10N NaOH. However, the phase of } the moon had changed as well.
Dear Margaret and Tina, Is it possible that CO2 reacted with the old NaOH? Is PbCO3 yellow? That would explain why fresh NaOH would work. Yours, Bill Tivol
Julie, You may want to look into the possibility that a local university or business has some older scopes that they would like to donate for your efforts.
Our local microscopy society OMS (Oklahoma Microscopy Society) was graciously granted 128 microscopes that were surplus for the University of Oklahoma. We sent out notices to the public schools in the state including an application form for the microscopes. We have since placed all 128 microscopes in a variety of elementary and secondary schools all over Oklahoma. The microscopes were old, although they were in good shape and many were fine binocular scopes with 4 objectives and good sub stage condensers. The lower elementary school grades (k-4) received scopes with 2 objectives and sub stage apertures. These scopes are perfect for the lower grades because of their simplicity, although they produce nice images and are very sturdy.
We are always on the lookout for more scopes as the demand from the schools exceed the supply. Greg
Julie Gross wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I am trying to help a Hartford, CT high school biology teacher find } microscopes for her classes. Any leads, ideas, advice for locating } microscopes? I'm in Connecticut but welcome input from anywhere. } } Julie Gross } Dept. of Neuroscience } University of CT Health Center } Farmington Ave. } Farmington, CT 06030 } jgross-at-neuron.uchc.edu
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
We have a student working with a protein which is inside microsomes. He would like us to immunolabel the protein with a polyclonal antibody to prove that it is inside the microsome and not a contaminant from the cytoplasm. I would appreciate any suggestions from all who have attempted this or something similar.
Thanks,
Michelle Peiffer ************************************************************* Electron Microscope Facility for the Life Sciences Penn State University Biotechnology Institute 001 South Frear Lab University Park PA 16802
In the Microscopy & Microanalysis journal of Microscopy Society of America, affiliated with Brazilian Society, there are e-mail contacts for SBMM. I suggest you start with these: sbmm.ceme-at-epm.br OR: microcel-at-biomed.icb2.usp.br
Good luck,
Gib Ahlstrand
P.S. I was just in your area a few weeks ago and enjoyed the nice cool weather! Its hotter than blazes in Minnesota right now. -------------------------------------------- Responding to the message of {4.1.20000811114014.009a4820-at-mail.cena.usp.br} from "adriana-at-cena.usp.br"-at-sparc5.microscopy.com:
} Would anyone have the web site of the "Sociedade Brasileira de Microscopia } e Microanalise - SBMM"? } Thanks in advance, } } Adriana } } Adriana Pinheiro Martinelli Rodriguez, PhD } Laboratorio de Biotecnologia Vegetal } CENA, Universidade de Sao Paulo } Av. Centenário 303, Cx. Postal 96 } 13400-970, Piracicaba, SP, Brasil } phone: +55-19- 429-4694 } fax: +55-19- 429-4610 } adriana-at-cena.usp.br } http://www.cena.usp.br/labs/labbiotecveg.htm } } } } } } } .
Gib Ahlstrand Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu http://biosci.umn.edu/MIC/consortium.html
Allied High Tech Products, Inc. has two different devices, the MultiPrep Precision Polishing System (Part No. 15-2000) and the TEM Wedge Polisher (Part No. 69-41000). The MultiPrep is an automated tool that is capable of thinning to samples 1 micron, as well as creating angles to 13 degrees. The TEM Wedge Polisher is a manual fixturing device that can be ste to remove material in mmicron incriments.
If you would like more information on this machine you can visit our website at www.alliedhightech.com or send me an e-mail directly.
I hope this answers your question.
Best Regards,
Armando Verdugo Laboratory Supervisor Allied High Tech Products, Inc. 2376 E. Pacifica Place Rancho Dominguez, CA 90220 (800)675-1118 US and Canada (310)635-2466 worldwide (310)762-6808 fax www.alliedhightech.com
The website address for the Sociedade Brasileira de Microscopia e Microan lise is at URL http://venus.rdc.puc-rio.br/sbme2/sbmeport.htm
It still seems to be mostly "under construction" but there is some information there.
Chuck
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It seems the northern hemisphere has collectively gone on leave - or to Philadelphia. Lets see if we can help, though its decades since I worked on starch, but those grains in seeds have not changed - I think.
Trying to fix dry seeds is near impossible and you should look at alternative methods, such as freeze etching or high resolution scanning, perhaps freeze substitution. If you can imbibe the tissue for a day or two, its still difficult. It was my notion that the GA just does not penetrate, regardless of fixation time. You may have some success by using GA at room temperature or warmer, but I doubt that. Using OsO4 you can after part processing actually see under a dissecting scope how far it has penetrated. Of course OsO4 is little use if you wish to do cytochemistry. Suggest that you use the OsO4 also at room and higher temperatures. Plant enzymes causing autolysis are fairly active in the cold and since in ice fixation times are perhaps 4x longer than at 20 degrees and maybe 8 times longer than at 37 degrees (impossible to truly quantify, but this gives the idea), shorter fixation times at higher temperatures are preferable. Do a fixation trial, at room temperature in OsO4 for say half, one and two hours and see what looks best. If the cytoplasm is dark (worse still membranes turn white) the tissue is overfixed. The granules will always tend to fall out during sectioning, but if the sections just simply fall apart, its most likely that the tissue was not fully fixed, infiltration is the less likely cause. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Friday, August 04, 2000 11:22 PM, ABM Siddique [SMTP:siddiqueabm-at-hotmail.com] wrote: } } Dear All: } } I have been trying to study the ultra-structure (TEM) of strach granules } (in wheat and maize seeds) that contains amorphus and crystaline layers. } Unfortunately, I am unable to look at these layers under TEM even after } keeping the tissues longer time in fixative (over night in } 3%gluteraldehyde) and embedding medium (LR white/Spurs for 2 days). } } I also tried the the above procedure with isolated starch granules in low } melted agar but the result is the same - poor penetration of embedding } resin as a result I am getting broken starch granules without any } ultrastructural details. } } I would appreciate it if anybody could suggest me how to obtaine a } reasonably good ultra-structural (TEM) details of starch granules. } } With thanks, } } Siddique } } } Get Your Private, Free E-mail from MSN Hotmail at } {http://www.hotmail.com/} http://www.hotmail.com } } }
I am a postgraduate at the department of Engineering at the University of Sheffield. My samples are transparent glass melts 4cm3 with inclusions in them. I am using both transmitting light and reflecting light microscopy.
I would like to obtain a x1 mag or a 1.25 mag, objective for the Olympus CH-2 microscope, with the following features: 1. The objective lens should have a short barrel i.e. the length of the objective, from the top [ where it screws into the objective holder] to the bottom of the objective [ DT] = {30mm, -the features of my sample are fairly large so I need an objective with a short barrel, giving me a maximum depth of field. This factor is particularly important. 2. At the top where it screws into the objective holder, diameter is 20.11mm 3. with an acromat plan lens corrected for flatness of field 4. the objective will be used without a cover glass and without an immersion fluid 5. preferably the objective will be infinity corrected. I am not sure whether a standardized adjustment length would be necessary in order to allow max depth of focus, whilst using transmitting microscopy
At present the objective used on the above microscope is a x 2.5 mag, x5 mag and a x10 mag. They have the following notations MSPlan x 2.5 mag / 0.07, infinity/ - f = 180, Olympus 100621 MSPlan x 5mag / 0.13, infinity/ - f = 180 , [ this has a short barrel = 30mm ]
I would welcome any help or advice regarding obtaining the above objective lens. .
Biological electron microscopists often fall into the trap of automatically fixing everything, even when it is inappropriate to do so. I was once asked what the best fix would be for diatom frustules, to which the answer is probably to see a doctor!. But seriously, I am not quite sure why it should be thought necessary to fix starch grains either with glutaraldehyde or with osmium, or what bearing such "fixation" would have on starch grains falling out of epoxy resin sections. Let's look at some fundamentals. Starch grains are mainly crystalline or paracrystalline assemblages of glucan. The molecules are therefore in a minimum-energy configuration, which will be extremely difficult for most other molecules to enter. Unless this crystalline order is severly disrupted no significant quantity of epoxy resin will enter the grains - infiltration is essentially impossible. Glutaraldehyde and osmium will not react with starch, so it is pointless attempting to fix them unless there are traces of proteins or lipids within the grain structure that must be stabilised. I really don't know how much protein or lipid is present within the crystalline starch - I am sure you'll tell me - but the main issue here is that the embedding resin is externalized by the crystalline structure of the grains, and does not bond too well with the surface either, so that the grain readily separates from the section. I think the possibility of promoting covalent bonding of the starch grain surface to the epoxy could be a more promising avenue to pursue than experimenting with different permutations of fixatives. There was some discussion in this forum some time ago about silanes manufactured by Dow Corning that can be used to promote adhesion of various materials to epoxies and other resins.
Chris Jeffree ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
Dear all, In my laboratory, we study vascular endothelium with electron microscopy (Guinea pig). We tried perfusion to fix tissue but endothelium was removed. We fix them with 4% paraformaldehyde + 0,1 % Gluta + 0,1 % picric ac in phosphate buffer pH 7,4 overnight. Artery are then embedded on unicryl for immunocytochemistry. Tissue was stain with uranyl acetate and Reynolds. With this procedure cell structure isnot very evident, membrane is well defined but not very contrasted. Ac locate on all the section, not very locate in a particular place of cells. We tried other first and second antibodies but it was the same thing. What can we do? Do you have any suggestion? Thanks
SEM MASTERCLASS THE SEM APPLICATIONS COURSE 3rd to 5th October 2000 THREE DAYS $600 AUS (Not including meals and accommodation)
An opportunity for a hands-on introduction or a more advanced session, depending on your requirements, on just about every one of the major applications of modern scanning electron microscopy:
Conventional SEM - FESEM - cold stage - EDXA- cathodoluminescence - variable pressure SEM- low kV - coating techniques - film and digital recording - tricks for difficult specimens.
When? Tues-Thurs 3rd, 4th, 5th October 2000
Where? The Australian National University Electron Microscopy Unit, Canberra, Australia
Who by? Steve Chapman (Protrain, UK) and ANUEMU staff
Who for? Anyone who feels they could get more out of their time on the SEM if they had a bit more background and knew more of the possibilities. Can your machine deliver what you need with a little coaxing? Or would different equipment make a big difference to your work? For biologists or materials scientists. Participants are encouraged to bring their own samples.
What with? FOUR SEMs will be used for the course: a Hitachi 4500 FESEM, JEOL 6400 SEM, Cambridge 360, Hitachi 2250-N SEM. Accessories include three EDXA detectors, several cold stages, and a number of different SE, BSE and CL signal detectors. For equipment details, see our website, http:/www.anu.edu.au/EMU
Queries: email stowe-at-rsbs.anu.edu.au . Course Registration form also on website http://online.anu.edu.au/EMU/00chapman.htm
Dr Sally Stowe Facility Coordinator, ANU EMU Box 475 Canberra ACT 2601 AUSTRALIA stowe-at-rsbs.anu.edu.au fax 61 (0)2 6249 3218 or 6279 8525
Does anyone have any experience of either/both of the Epson Expression 1600 Pro or the Minolta Dimage Multi scanners? One thing we would like to do is to scan negatives at oblique angles, rather than parallel to the film strip. My understanding is the even medium format scanners constrain the negative in a holder, whereas using a flat bed with a transmission attachment (such as the Epson) would allow rotation.
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
I. Why don´t you try it with 1% aqueous UAc. Either for twenty minutes a room temperature or at 40°C for 7-10 minutes (results in more intense contrast...)
II. For staining with alcoholic UAc we apply the following procedure: 1. 20 min. 1% UAc in 70% alc. 2. wash: 1-2 drops 70% alc. 3. 1-2 drops 50% alc. 4. 3-4 drops aqua dest. 5. dry on filter paper.
You wrinkling problem might be due to a harsh change from alcohol to water, which causes damage to the sections. And, are your sections supported on the grids by a formvar or carbon film?
For Spurr´s resin I would prefer the warm, aquaeous method. Hope this works.
Greetings, Michael
} } I have a constant problem of regularly spaced wrinkles on my Spurr } sections on the TEM. I stain with uranyl acetate in 50% ethanol for } about 30 minutes followed by stable lead for 15 minutes.(Wrinkling is } even worse with methanolic UA). I am certain the problem has something } to do with staining since few wrinkles appear when I observe unstained } sections. I also rarely encounter wrinkled sections with Embed 812 } sections when using aqueous UA stains. Any suggestions? } } Tony Greco } }
I apologise if this is a little out-of-gamut for this list, but I have been trying to make digital copies of archival material which only exists as small prints on lustre paper. On a flat bed scanner, the textured surface causes objectionable specular reflections which degrade image quality. Is there a good way to avoid this?
Chris ========================================== Dr. Chris Jeffree University of Edinburgh BIOSEM - Biological Sciences Electron Microscope Facility Institute of Cell and Molecular Biology Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 (0) 131 650 5345 FAX. #44 (0) 131 650 6563 Mobile 07710 585 401 email c.jeffree-at-ed.ac.uk =========================================
Are there problems with the system or did I somehow get deleted from the system? I haven't received any mail since last Wednesday (8/9/00). If I got deleted please re-subscribe me. Thank you.
prutledge-at-ars.usda.gov
Phil Rutledge USDA/ARS 151 Dixon Drive Chestertown, MD 21620 voice: 410.778.4136 or 2120 fax: 410.778.4399
} I apologise if this is a little out-of-gamut for this list, but I have been } trying to make digital copies of archival material which only exists } as small prints on lustre paper. On a flat bed scanner, the textured } surface causes objectionable specular reflections which degrade } image quality. Is there a good way to avoid this?
Dear Chris, I have had excellent luck with old photos by re-photographing them. The lighting has to be arranged so that there are no spurious reflections, but this is not too difficult. Using a digital camera will make scanning the photos unnecessary, and using film will give you a high-resolution negative. You will probably want to add a scale bar to the original to account for changes in magnification. You also need to take care that both the center and the edges of the print are in focus, since they can be at significantly different distances from the lens. Good luck. Yours, Bill Tivol
is there a detailed and comprehensive reference (with em pictures) to coiled bodies, pol II transcriptosomes (and pol I and pol III (?)), interchromatin granules, perichromatin fibrils, peripheral condensed chromatin, and of perinucleolar chromatin, fibillar centers, dense fibrillar component, granular component .....and other that someone knows of?????i would be eternally grateful for a lead to such a paper(s) Thanks you, marian miller
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The only EM-type ref I know of is from 1990; Raska et al. in Electron Microsc. Rec., 3:301-353. I can make a photocopy of my photocopy if you are interested, but the images are mush. it is called : "Immunocytochemistry of the Cell Nucleus".
The other refs I know of are mostly text and LM/FM, not TEM. You may have to just lookup the individual authors who work on these stuctures...I don't know if there are any sectioned images of transcriptosomes, but the others are all out there. See papers from the labs of David Spector, Robert Ochs, Marc Thiry, Daneholt, Aebi....There is a review from 1993 by Spector that talks about the regions and has refs to EM papers: "Macromolecular Domains within the Cell Nucleus" Annu. Rev. Cell Biol., 1993 9:265-315.
Hope these help! If anyone sends you the ref for a good EM review, could you please post it to the server?
Tamara Howard CSHL (IGC-land)
On Mon, 14 Aug 2000, marian miller wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } is there a detailed and comprehensive reference (with em pictures) to } coiled bodies, pol II transcriptosomes (and pol I and pol III (?)), } interchromatin granules, perichromatin fibrils, peripheral condensed } chromatin, and of perinucleolar chromatin, fibillar centers, dense } fibrillar component, granular component .....and other that someone } knows of?????i would be eternally grateful for a lead to such a } paper(s) Thanks you, marian miller } } }
With just one picture, you can usually retouch in Photoshop to restore the image. If you have many, then it is probably worth re-photographing them in flat light and through a polarizing filter. This should remove the reflections, but it can also will increase contrast, causing loss of detail in the very light and very dark areas of the image
I am posting this question for a colleague who is having trouble with the large size of his samples.
"I am trying to find a way to fix and embed calcified carotid endarterectomy specimens such that I will be able to obtain serial cross sections. The problem that I am encountering thus far is that I am unable to embed the 5cm specimens in a large paraffin block to allow sectioning because such large blocks either
a) do not fit on standard microtomes and therefore must be cut with sliding microtomes resulting in more distortion of the specimen
b) fall off of the blocks after 1 or 2 sections are obtained because the force of the blade against the mounted specimen shears the embedded specimen from the cassette or resin embedding block.
How can I fix and embed large specimens (~5cm long) such that I can obtain serial cross sections of the entire specimen for reconstructions without having to chop the specimen into small labeled pieces first and embed each of these pieces individually?"
Thank you ******** Cynthia J. Zeissler Physical Scientist National Institute of Standards and Technology cynthia.zeissler-at-nist.gov Tel. 301-975-3910 FAX. 301-417-1321
Cynthia: We routinely cut 3.5 to 4.0 cm paraffin blocks and cut some that exceed 5 cm in length and width on a rotary microtome. The limitation is the length of the up and down travel on your microtome. You can use a vise -type holder to hold these blocks. You can always have an adapter made if you can't purchase one that will hold large paraffin blocks. You might not want to use cassettes for this purpose but simply make a large stiff paper mold in the shape of a cube to hold the specimen like all the old original paraffin specimens were done. As far as cutting calcified blocks this may be impossible in paraffin under some circumstances if the blocks have not been decalcified first...depends on the amount of calcium. Serial sections will be a real challenge on specimens of this size and character and do not permit ready starting and stopping once commenced.
I know this may be a bit delayed (holidays you know) but maybe I can add a few points.
The micron marker is powered by the same electronics that produce magnification i.e. an error in magnification equals an error in micron marker too!
I do not know how old your instrument is or what make but this does make a considerable difference in the accuracy of magnification. It is "normal" to hope to get within 10% of the readout with an X to Y difference of less than 5%. If the following rules are not followed errors of up to 25% are quite easily obtained.
1. The sample must be a fairly flat surface
2. The sample must not be tilted
3. Turn OFF all electronic magnification and focus devices - dynamic focus, scan rotation, tilt correction
Use a 2160 lines/mm metal or TEM standard (a carbon grating replica, much cheaper and they work very well [see Spi]) to calibrate the machine but be aware that the spot size setting will have an influence upon the magnification accuracy!
The most accurate method for sample to sample consistency is to use the focus control as little as possible and use the Z control to obtain focus. In older instruments there was no zoom magnification linked to focus, so change the focus and you change the magnification. On modern instruments zoom magnification linked to focus is used in an attempt to hold the magnification correct.
After many years of testing instruments of all types, with clients on our courses there is no doubt that modern instruments are very good in relation to magnification accuracy, but some of the older machines were real dogs!
Good luck
Steve Chapman
Senior Consultant Protrain Tel & Fax - 44 (0)1280 814774 E-mail- protrain-at-emcourses.com Web Site - www.emcourses.com Protrain for SEM, TEM & EDX Training World Wide with our own full time Professional staff Courses in Australia, Canada, Europe, New Zealand, South Africa, Taiwan, UK and USA
You did not state the thickness of sections you are try to cut, this information could be important.
} From what you state, the only thing you have not tried is using a cryostat, this would give you a stable specimen without being as hard as using resin, our cryostats are routinely sectioning undecalcified bone, the 5cms. size would not pose a problem either.
Best Regards
Alan Bright
Bright Instrument Co.Ltd. St Margaret's Way Huntingdon PE18 6EB England
Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: AlanBright-at-brightinstruments.com Web Site: www.brightinstruments.com
-----Original Message----- } From: Cynthia J. Zeissler [mailto:cynthia.zeissler-at-nist.gov] Sent: 14 August 2000 21:12 To: Microscopy-at-sparc5.microscopy.com
I am posting this question for a colleague who is having trouble with the large size of his samples.
"I am trying to find a way to fix and embed calcified carotid endarterectomy specimens such that I will be able to obtain serial cross sections. The problem that I am encountering thus far is that I am unable to embed the 5cm specimens in a large paraffin block to allow sectioning because such large blocks either
a) do not fit on standard microtomes and therefore must be cut with sliding microtomes resulting in more distortion of the specimen
b) fall off of the blocks after 1 or 2 sections are obtained because the force of the blade against the mounted specimen shears the embedded specimen from the cassette or resin embedding block.
How can I fix and embed large specimens (~5cm long) such that I can obtain serial cross sections of the entire specimen for reconstructions without having to chop the specimen into small labeled pieces first and embed each of these pieces individually?"
Thank you ******** Cynthia J. Zeissler Physical Scientist National Institute of Standards and Technology cynthia.zeissler-at-nist.gov Tel. 301-975-3910 FAX. 301-417-1321
Has anyone used the program called "Zip Shot"? I have a 1830 Amray SEM with a Noran Sigma EDS System. I would like to be able to print out my results using this program. I am not familiar with Zip Shot, it has been recommended to me. Thanks!
} } We have a AISI4340 steel sample. This sample contains iron, carbon and } nickel. We ion milled the sample. When we put the sample into our TEM, } we cannot align or focus the microscope. we are operating a JEOL100CX } microscope. } Any suggestions for imaging this sample? } Thank you Won-Sik Kim
thanks to everyone who sent me information about SBMM (Brazilian Society of Microscopy and Microanalysis)
Their current website and e-mail are:
http://www.dema.ufscar.br/sbmm/
sbmm-at-power.ufscar.br.
The website of the SBMM - Regional Chapter Rio de Janeiro is:
http://venus.rdc.puc-rio.br/sbme2/sbmeport.htm (currently under construction)
Adriana
Adriana Pinheiro Martinelli Rodriguez, PhD Laboratorio de Biotecnologia Vegetal CENA, Universidade de Sao Paulo Av. Centenário 303, Cx. Postal 96 13400-970, Piracicaba, SP, Brasil phone: +55-19- 429-4694 fax: +55-19- 429-4610 adriana-at-cena.usp.br http://www.cena.usp.br/labs/labbiotecveg.htm
Have not heard of ZipShot. Have used HyperSnap for windows as a screen capture. HS seems to be flexible and works nicely. OTOH... I have forgotten if the Sigma is running W95/8... If so have you tried ALT-PrintScreen? This will capture the displayed image to the clipboard (at the current monitor resolution). You can then paste into an image editor like Photoshop...
Woody White Mcdermott Technology Inc.
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi everyone,
Has anyone used the program called "Zip Shot"? I have a 1830 Amray SEM with a Noran Sigma EDS System. I would like to be able to print out my results using this program. I am not familiar with Zip Shot, it has been recommended to me. Thanks!
Since this material is magnetic you will need to reduce the amount of material that you put in the microscope. The first cut is to just make the 3 mm disk thinner at the start, perhaps 100 µm or less. At our lab we also use undersized (1mm or 2.3 mm) specimens and occasionally embed them in another material to make up the 3mm before we thin them. Probably the best way is to use the "window" polishing technique (see Hirsch et al.) which minimizes the amount of material while maximizing thin area. However I find it difficult to do correctly.
If you contact me offline I can put you in touch with the person at our lab who does the most of this type of work. By the way, we also order our microscopes with "extra strength" objective stigmators for working with magnetic materials.
Cheers, John Vetrano john.vetrano-at-pnl.gov
---------- } From: Won Sik Kim Sent: Tuesday, August 15, 2000 6:50 AM To: microscopy-at-sparc5.microscopy.com
} } We have a AISI4340 steel sample. This sample contains iron, carbon and } nickel. We ion milled the sample. When we put the sample into our TEM, } we cannot align or focus the microscope. we are operating a JEOL100CX } microscope. } Any suggestions for imaging this sample? } Thank you Won-Sik Kim
Does anyone have a light microscope for sale appropriate for a first year medical student? Student lives in Jacksonville FL Will attend med school in Los Angeles. Please respond off line:
Becky Garrison Pathology Supervisor ph 904-549-6237 fx 904-549-4290
I am trying to find a web or e-mail address for "Nordic" an antibody supplier in Tilburg Netherlands. My web searches haven't worked. Anyone out there deal with this supplier of immunocytochemical antibodies, etc. Thanks, tom
-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
It sounds as if your sample may have become magnetized slightly. That is exactly what happened with my JEOL 1200EX-2 when I tried to examine iron particles which were slightly magnetic. I took the specimen grid and placed it into the coil I use to demagnetize my forceps with, and that solved the problem.
Franklin Bailey University of Idaho Electron Microscopy Center Moscow, ID 83844-2204 jfb-at-uidaho.edu
Reducing the size of your magnetic sample as John Vetrano has suggested is the best start. Here are some other things to consider and to try with the JEOL 100CX. (This question was last discussed on the listserver a year or so ago as I recall).
1. Be sure the sample is firmly held in the side-entry sample holder so it doesn't get pulled out by the magnetic field of the objective lens in the microscope. Sample holders with a spring-clip clamping mechanism are especially prone to this unpleasantness.
2. If you are using a double-tilt holder, check the mechanism for looseness to avoid having the sample spontaneously tilt to a position where the holder cannot be withdrawn from the microscope without scraping the polepiece or aperture holder.
3. Insert and withdraw the sample holder with the microscope in the low-magnification mode (objective lens off) to help avoid problems #1 and 2.
4. Pre-align the microscope in darkfield mode with a normal (nonmagnetic) sample before inserting the magnetic one. The available range of beam tilt correction in the 100CX microscope is much larger in the darkfield image control mode than it is in brightfield. A strongly magnetic sample will affect the beam tilts and image stigmation, but not the image focus.
5. Correct the alignments as follows: (a) Focus the image with the mechanical Z-axis translation rather than with the objective lens focus. (b) Align the beam tilts by voltage centering the image (darkfield mode to get enough tilting range). (c) Stigmate the image near its center --preferably with an unused stigmator channel so the normal one is not messed up for other users.
6. Repeat the alignment (#5) each time you tilt the sample or translate it a long way. The alignment corrections will increase a lot when the sample is tilted beyond ~5 degrees off horizontal, but should be manageable at most tilts unless the sample is too thick.
Larry Thomas larry.thomas-at-pnl.gov
From: Vetrano, John S Sent: Tuesday, August 15, 2000 10:31 AM To: 'Microscopy Listserver' Cc: 'kimwonsi-at-msu.edu' Subject: FW: AISI 4340 steel in TEM (fwd)
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
Since this material is magnetic you will need to reduce the amount of material that you put in the microscope. The first cut is to just make the 3 mm disk thinner at the start, perhaps 100 µm or less. At our lab we also use undersized (1mm or 2.3 mm) specimens and occasionally embed them in another material to make up the 3mm before we thin them. Probably the best way is to use the "window" polishing technique (see Hirsch et al.) which minimizes the amount of material while maximizing thin area. However I find it difficult to do correctly.
If you contact me offline I can put you in touch with the person at our lab who does the most of this type of work. By the way, we also order our microscopes with "extra strength" objective stigmators for working with magnetic materials.
Cheers, John Vetrano john.vetrano-at-pnl.gov
---------- } From: Won Sik Kim Sent: Tuesday, August 15, 2000 6:50 AM To: microscopy-at-sparc5.microscopy.com Subject: AISI 4340 steel in TEM (fwd)
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
} } We have a AISI4340 steel sample. This sample contains iron, carbon and } nickel. We ion milled the sample. When we put the sample into our TEM, } we cannot align or focus the microscope. we are operating a JEOL100CX } microscope. } Any suggestions for imaging this sample? } Thank you Won-Sik Kim
Can someone advise me the appropriate temperature for regeneration of molecular seive material used in a foreline trap in a diff pump/rotary pump system on an SEM?
tia
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
} The following announcement is posted for: } } Roger Reyes, Group Leader } Failure Analysis Lab } International SEMATECH } 2706 Montopolis Drive } Austin, TX 78741 } (512) 356-3842 } roger.reyes-at-sematech.org } } To apply, please contact Roger directly. } ********************************************** } JOB TITLE: FA Engineer } } JOB SUMMARY: To provide critical support to the Advanced Tool Development } Facility (ATDF) and International SEMATECH's projects. } } JOB RESPONSIBILITIES: } * Continuously improve quality and accuracy of lab analysis and general } lab cycle time. } * Engineer would work on jobs that are beyond the technicians level, } providing in-depth analysis and advice on complex problems. } * Engineer would be working closely with technicians so that learning and } development occurs. } * Engineer would attend technical staff meetings. } * Some report writing and technical presentations. } * Engineer will keep pace with the leading edge technology. } } QUALIFICATIONS: } * Engineering degree. } * Experience in the semiconductor industry is preferred. } * Knowledge with databases. } * Knowledge on wet chemistries to delineate CMOS layers. } * Knowledge of Failure Analysis tool set. ( such as SEM, FIB,CVD, RIE } etc...) } * Good people skills. } } } }
Ramin Rahbari Pfizer Global Research & Development Drug Safety Evaluation 2800 Plymouth Road Ann Arbor, MI 48105 Voice (734) 622-3383 Fax (734) 622-5001 Ramin.Rahbari-at-Pfizer.com
-----Original Message----- } From: Tom Phillips [mailto:PhillipsT-at-missouri.edu] Sent: Tuesday, August 15, 2000 4:28 PM To: Microscopy-at-sparc5.microscopy.com
I am trying to find a web or e-mail address for "Nordic" an antibody supplier in Tilburg Netherlands. My web searches haven't worked. Anyone out there deal with this supplier of immunocytochemical antibodies, etc. Thanks, tom
-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
I would be grateful for feedback offline from any users of the new(ish) JEOL 6700 FESEM, which appears still to be rather rare in UK.
Many thanks Chris ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
Hello I am looking for a good source for gelatin for cryo-immunogold experiments. In the many papers on the subject I cannot find where the authors ordered the gelatin or any purity information. Any information would be appreciated. Thank you Theresa Theresa A. Fassel Core EM Unit, Mail Box MB-32 The Scripps Research Institute 10,550 N. Torrey Pines Rd. LaJolla, CA 92037-1027 tel: (858)-784-8182 fax: (858)-784-8193 tfassel-at-scripps.edu
I am looking for one each Zeiss KPl-W 10X eyepiece, catalog no. 46 40 42. Have just checked eBay with no success. does anyone have one for sale or can anyone suggest a source? Thanks, Mary Pfauth
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am looking for one each Zeiss KPl-W 10X eyepiece, catalog no. 46 40 42. } Have just checked eBay with no success. does anyone have one for sale or } can anyone suggest a source? Thanks, Mary Pfauth
I may have a spare in the lab. I'll check tomorrow.
Please look at the Project Micro Pages on How to buy microscopes. There is alot of valuable advice there.
http://www.msa.microscopy.com/ProjectMicro
I will also be forwarding your Email to Caroline Schooley (schooley-at-mcn.org) as well as the Microscopy Listserver. Caroline will have the absolute latest information from the National Microscopy meeting which a large fraction of us from the US Microscopy Community are at this week. The Listserver members will be able to advise you on the rest of your questions, I have never used this microscope so cannot give you good advise on it's purchase.
Nestor
} } Email: jfallos-at-hotmail.com } Name: Janet Jacobson-Fallos } School: Bliss Discovery School } } Question: Hello! } } First, I need to tell you that I'm the TEACHER! :) } I haven't done anything with a microscope since college Biology 101. I am } hoping you might be able -- and willing -- to help me... } } I have been looking for quite some time to purchase a simple, yet high } quality microscope for use by highly-motivated middle school-aged } students. To find something of investment quality -- yet on a teacher's } out-of-pocket budget, I decided to look for a used Zeiss, Leica, Nikon or } Olympus. I had hoped to obtain a microscope with a 10x eyepiece and 4 } objectives -- incl. a 100x oil immersion. I've had a difficult time } finding anything under $250.00 } } I now have the opportunity to purchase an Olympus Model K ; circa 1970's?; } w/ a 10x eyepiece and 10x, 40x and 100x objectives; X,Y slide mechanism, } course and fine adjustments with locks. It has no illuminator, so I would } need to purchase one separately. The price is $140. I have seen used } illumination kits for anywhere from $40. -$200. I don't know if the 100x } objective on the microscope is designed for oil-immersion or not. (I am } currently awaiting the seller's answer to that question...) The microscope } doesn't come with any accesories -- no dust cover, case, slides, manuals, } books, etc. :( } } If you are familiar with Olympus microscopes, and hopefully the K model in } particular, I would appreciate any information/comments you might provide - } } ---------------------------------------------------------------------------
I was wondering if anyone could provide some information on tensile test fixtures for SEM's. Particularly if you have one and if you like or dislike it. I also need some manufacturers. The holder will be for a Hitachi 3200N microscope. Thanks. ______________________________________________ Roberto Garcia Senior Analyst, Metallography NC State University / Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh, NC 27695-7531 (919) 515-8628 (919) 515-6965 Fax rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif http://spm.aif.ncsu.edu/asm/asm_home.htm ____________________________________________
I am looking for a copy of: Lewis J, Nelson J, Elder H: Radioimmunoassay of an antibiotic: gentamicin. Nature [New Biol] 239:1128, 1969 If anyone has this old paper sitting around please respond by email.
Thank you Karen Kelley Senior Electron Microscopist UF Biotechnology Electron Microscopy Core Lab Box 118525 Gainesville Florida 32611 lab:352-392-1184 fax: 352-846-0251 email: klv-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/staff/karenpage.html
Dear Valerie, per se your protocol looks pretty good. I have three suggestions. Fix it like that, try again perfusion fixation because it would preserve endothelium at best. Maybe you can even omit the Glut. Second, try tannic acid, this enhances contrast while preserving antigenicity. Third, try either LR-White resin or LR-Gold. Don´t cure it in the oven, use the accelerator in the cold (-20°C). I am dealing with immunoelectronmicroscopy on endothelia for over two and a half years now and we got now the breakthrough in finding a balance between structure preservation and antigenicity.
If you would like to discuss this in more detail, don´t hesitate to contact me.
michael.reiner-at-uni-koeln.de Michael Reiner University of Cologne, Germany Dept. of Anatomy I
} Valerie wrote: } Dear all, } In my laboratory, we study vascular endothelium with electron microscopy } (Guinea pig). We tried perfusion to fix tissue but endothelium was removed. } We fix them with 4% paraformaldehyde + 0,1 % Gluta + 0,1 % picric ac in } phosphate buffer pH 7,4 overnight. Artery are then embedded on unicryl for } immunocytochemistry. Tissue was stain with uranyl acetate and Reynolds. } With this procedure cell structure isnot very evident, membrane is well } defined but not very contrasted. Ac locate on all the section, not very } locate in a particular place of cells. } We tried other first and second antibodies but it was the same thing. } What can we do? } Do you have any suggestion? } Thanks } }
Michael Reiner University of Cologne, Germany Dept. of Anatomy I _______________________________________________________________________ 1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de
A well-meaning, non-microscopist friend to whom I have rented out a room in my lab decided to spiffy up the place while I was out of town. He tipped the roughing pump to a JEOL 35 up on end and stood it in the corner. When I got back from my trip, there was, of course, oil all over the floor.
The microscope was not in service at the time, but I want to place it in service as a back-up SEM some time in the future. My question:
What do I need to do (other than replace the lost oil) before starting up the pump?
In our lab we use a 275 bloom gelatin from fisher. Catalog number G8-500. We use a 2% solution. We went through many gelatins and finally was recommended to use the 275 bloom. Good luck:)
Roberto, We have a tensile stage with 500 degC heating capability which was built for us by Oxford Instruments (that division may now be part of Gatan?). The stage is for a LEICA S440 though I believe they could adapt to it for other microscopes. The system is good and has worked well. Good luck, Simon
} } } "Roberto Garcia" {rgarcia-at-unity.ncsu.edu} 17 August 2000 13:47:19 } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I was wondering if anyone could provide some information on tensile test fixtures for SEM's. Particularly if you have one and if you like or dislike it. I also need some manufacturers. The holder will be for a Hitachi 3200N microscope. Thanks. ______________________________________________ Roberto Garcia Senior Analyst, Metallography NC State University / Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh, NC 27695-7531 (919) 515-8628 (919) 515-6965 Fax rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif http://spm.aif.ncsu.edu/asm/asm_home.htm ____________________________________________
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POST DOCTORAL FELLOW - ROOT/RHIZOSPHERE BIOLOGIST $46K - $52K PLUS SUPERANNUATION
CSIRO PLANT INDUSTRY
Canberra Australia
We are seeking a motivated Postdoctoral Fellow to join a team of researchers in a project with the overall aim of developing a conceptual framework for understanding the role of root/rhizosphere biology in crop productivity. Specifically you would be investigating the impacts of different crop management practices (e.g. conservation cropping) on root architecture and structure, and on the in situ localisation and composition of rhizosphere microbes. The work will involve the use of modern methods of microscopy and bacterial identification and will also involve collaboration with other soil biologists, agronomists and farmers.
The successful applicant will have a PhD and experience with root and soil biology, preferably with field-grown plants, and technical expertise and/or interest in the practical application of modern microscopy and microbiological methods to the research project.
This position is for a term of 3 years, with possible extension to 5 years.
This post-doctoral fellowship is sponsored by the Grains Research Development Corporation as a component of a major new initiative in Soil Biology Research. The position is within the CSIRO Division of Plant Industry. The Division has a staff of 700 with 60% located at its headquarters in Canberra and other groups located at seven sites across Australia.
For further information contact Professor Margaret McCully (email mccully -at-pi.csiro.au ; phone 61 2 6246 5343) or Dr John Kirkegaard (email j.kirkegaard-at-pi.csiro.au ; phone 61 2 6246 5080) An Information Package and Selection Documentation can be obtained by contacting the answering service on 61 2 6246 5434 or fax 61 2 6246 5000 or email recruit-at-pi.csiro.au This information is also available via our web site http://www.pi.csiro.au/
Applicants must obtain and respond to the Selection Criteria and Duty Statement. Address your application for the above position quoting Reference No. PG:00082 and include details of your experience, skills and qualifications together with the names of 3 references. Please mark 'Confidential' and forward to: Trish Borger, Human Resources, CSIRO Plant Industry, GPO Box 1600, Canberra ACT 2601 by Sepy. 15/ 2000 (notional).
Deben Research manufacture an excellent range of micro-testing equipment for SEM chamber operation. Their details are :
Deben Research 11-15 High St Stowmarket Suffolk IP14 6QL UK Tel: +44 1728 861320 Fax: +44 1728 861350 Email: info-at-deben.co.uk WWW: http://www.deben.co.uk
Cheers,
David Vowles Electron Microscopy Unit Dept of Materials Science and Metallurgy University of Cambridge Pembroke St Cambridge UK CB2 3QZ Tel: +44 1223 334325 Fax: +44 1223 334567 Email: djv23-at-msm.cam.ac.uk
On Thu, 17 Aug 2000, Roberto Garcia wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I was wondering if anyone could provide some information on tensile test } fixtures for SEM's. Particularly if you have one and if you like or dislike } it. I also need some manufacturers. The holder will be for a Hitachi 3200N } microscope. Thanks. } ______________________________________________ } Roberto Garcia } Senior Analyst, Metallography } NC State University / Analytical Instrumentation Facility } Campus Box 7531 Room 318 EGRC } 1010 Main Campus Dr. } Raleigh, NC 27695-7531 } (919) 515-8628 } (919) 515-6965 Fax } rgarcia-at-unity.ncsu.edu } http://spm.aif.ncsu.edu/aif } http://spm.aif.ncsu.edu/asm/asm_home.htm } ____________________________________________ } } }
My research department is putting in a grant to ask money to buy a new set of microscopes which should work well with both bright and dark field, and coupled with color digital camera, and image analyzer. So far, we have got a quote from ZEISS for around $77K. My director wants to have the best, easy to use, easy to upgrade with great service for years to come.
Some has told us that ZEISS has the best lens quality in the world, and others said NIKON lenses are compatible to ZEISS, and its cameras are superior. Again others would recommend LEICA because it also makes excellent instruments for less money, with great service in Tampa Bay area.
Please help with your experience and knowledge in this field. Any satisfied customers? We don't have to buy the whole set from one company. We can get microscopes from one, digital camera from others.
We work most with Bone and Cartilage, paraffin, frozen, tissue culture, and plastic, if this information will have any weight on choosing which company we should go for.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } } POST DOCTORAL FELLOW - ROOT/RHIZOSPHERE BIOLOGIST } $46K - $52K PLUS SUPERANNUATION } } CSIRO PLANT INDUSTRY } } Canberra Australia } } } } We are seeking a motivated Postdoctoral Fellow to join a team of } researchers in a project with the overall aim of developing a conceptual } framework for understanding the role of root/rhizosphere biology in crop } productivity. Specifically you would be investigating the impacts of } different crop management practices (e.g. conservation cropping) on root } architecture and structure, and on the in situ localisation and composition } of rhizosphere microbes. The work will involve the use of modern methods } of microscopy and bacterial identification and will also involve } collaboration with other soil biologists, agronomists and farmers. } } } The successful applicant will have a PhD and experience with root and soil } biology, preferably with field-grown plants, and technical expertise and/or } interest in the practical application of modern microscopy and } microbiological methods to the research project. } } This position is for a term of 3 years, with possible extension to 5 years. } } This post-doctoral fellowship is sponsored by the Grains Research } Development Corporation as a component of a major new initiative in Soil } Biology Research. The position is within the CSIRO Division of Plant } Industry. The Division has a staff of 700 with 60% located at its } headquarters in Canberra and other groups located at seven sites across } Australia. } } For further information contact Professor Margaret McCully (email mccully } -at-pi.csiro.au ; phone 61 2 6246 5343) or Dr John Kirkegaard (email } j.kirkegaard-at-pi.csiro.au ; phone 61 2 6246 5080) An Information Package } and Selection Documentation can be obtained by contacting the answering } service on 61 2 6246 5434 or fax 61 2 6246 5000 or email } recruit-at-pi.csiro.au This information is also available via our web site } http://www.pi.csiro.au/ } } Applicants must obtain and respond to the Selection Criteria and Duty } Statement. Address your application for the above position quoting } Reference No. PG:00082 and include details of your experience, skills and } qualifications together with the names of 3 references. Please mark } 'Confidential' and forward to: Trish Borger, Human Resources, CSIRO Plant } Industry, GPO Box 1600, Canberra ACT 2601 by Sepy. 15/ 2000 (notional). } } } } }
Several people requested copies of the full paper I presented at the Philadelphia meeting on the characterization of surface topography using AFM and SEM. I have uploaded a 720 kB "pdf" (acrobat) file containing a summary of my verbal presentation with images of the slides, for anyone interested. You can download it at {http://personal.rdu.bellsouth.net/~jcr5/MSA_paper.pdf}
I own a Zeiss, a Nikon and 2 Olympus scopes. Which is best? Depends on the application. Brand X may have the greatest optics but if the price prevents you from buying all the types of objectives needed for different approaches, then you will end up making do with the "wrong" objective and lose any real gain. The 3 manufacturers are all very similar. There are subtle advantages for each type (e.g., Nikon has the best working distance objectives). First, decide what features on objectives you need (e.g., water immersion, high NA, working distance, high UV thruput) and then buy the one you can afford.
Service is a function of your local rep and the company has very little to do with it in my experience. Zeiss owns their reps these days but it is still the local guys who you deal with.
The digital camera can come from any vendor - the best ones are probably not made my the microscope companies.
That's my prejudiced two cents worth!
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
Pbgrover-at-aol.com-at-sparc5.microscopy.com wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } A well-meaning, non-microscopist friend to whom I have rented out a room in } my lab decided to spiffy up the place while I was out of town. He tipped the } roughing pump to a JEOL 35 up on end and stood it in the corner. When I got } back from my trip, there was, of course, oil all over the floor. } } The microscope was not in service at the time, but I want to place it in } service as a back-up SEM some time in the future. My question: } } What do I need to do (other than replace the lost oil) before starting up the } pump? } } Paul Grover Paul, After you finish cleaning up the oil, turn the pump by hand a couple of turns just to be sure all is OK. It should be fine.
Ken Converse owner Quality Images third party SEM service Delta, PA
Good points. I am using Zeiss Axioskops despite near zero help from Zeiss. I used Olympus for years. But the newer BX series did not produce the images I need. Olympus was/is user friendly. Zeiss is user hostile as I see it. But their equipment is very good.
It takes a while to get the systems to work when there is little or no help from the maker. This is a result of their cancellation of dealers. Some Zeiss items can be found at great prices from abandoned Zeiss dealers.
gary g.
At 01:30 PM 8/18/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
You might also want to check your exhaust manifold and change the filter - just in case it was contaminated by some of the oil.
-- Lola Norton Research Engineer ESEM Facility EDU-114 (915) 747-8049
University of Texas at El Paso Chemistry Department Mail Code:00513 El Paso, TX 79968
} From: Ken Converse {qualityimages-at-netrax.net} } Organization: Quality Images } Reply-To: qualityimages-at-netrax.net } Date: Fri, 18 Aug 2000 20:20:34 -0700 } To: "Pbgrover-at-aol.com"-at-sparc5.microscopy.com } Cc: Microscopy-at-sparc5.microscopy.com } Subject: Re: roughing pump tipped over } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Pbgrover-at-aol.com-at-sparc5.microscopy.com wrote: } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } A well-meaning, non-microscopist friend to whom I have rented out a room in } } my lab decided to spiffy up the place while I was out of town. He tipped the } } roughing pump to a JEOL 35 up on end and stood it in the corner. When I got } } back from my trip, there was, of course, oil all over the floor. } } } } The microscope was not in service at the time, but I want to place it in } } service as a back-up SEM some time in the future. My question: } } } } What do I need to do (other than replace the lost oil) before starting up the } } pump? } } } } Paul Grover } Paul, } After you finish cleaning up the oil, turn the pump by hand a couple of } turns just to be sure all is OK. It should be fine. } } Ken Converse } owner } Quality Images } third party SEM service } Delta, PA } } }
Several folks have made good points on this, so I am throwing in my two cents as well. There are many considerations for defining a "good" microscope. Personal preference (ergonmetrics, habits), price, and most importantly the task/procedures it is used for. You dont use a student microscope for R&D or vice versa.
When I consider a microscope purchase, I look only at the universals....we are scientists and use them accordingly, even in production settings. If I was a teacher, I would chose differently. My personal choice over the last 7 years is to go with the Oly BMAX line. I never cared for Oly until they got their act back together with the introduction of that line. It is a modification of Zeiss lineage. Zeiss still has the best objectives, but it is moot. Most of that difference you cannot resolve with your eyes.....just arent capable of doing so. So, I choose Oly objectives or used Zeiss objectives.
One last note. The russian scopes are incredibly enticing at their price, and they are pretty good in quality. My only problem with them is they are still built like their tanks.
Lou Solebello ----- Original Message ----- } From: Gary Gaugler {gary-at-gaugler.com} To: Tom Phillips {PhillipsT-at-missouri.edu} Cc: MSA listserver {Microscopy-at-sparc5.microscopy.com} Sent: Friday, August 18, 2000 6:13 PM
A one day TEM short course will be offered through the American Vacuum Society in Boston on Oct. 2, 2000.
For details please see: www.vacuum.org
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Mechanical, Materials, and Aerospace Engineering Director, UCF/Cirent Materials Characterization Facility University of Central Florida 12443 Research Parkway, Suite 305 Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
I have inherited a Montage FR2 film recorder which looks to be in great shape except that I have been unable to figure out how to drive it and technical support for this product appears to be non-existent. Does anyone know how to interface the FR2 with a Mac, to generate slides from PowerPoint or Canvas?
Thanks
Gary Ward
Department of Microbiology and Molecular Genetics 214 Stafford Hall University of Vermont Burlington VT 05405 USA
I have looked at microscopes from most of the main suppliers and I have yet to find one that compares to the Leica range for quality (both optical and build) and, as far as I am concerned, ease of use and ergonomics. I use at the moment an Olympus AX which is excellent optically but is a nightamare to use. Nothing is easy to get to and every time I move my hand from the focussing or lens changer, I catch the stage even after two years of using the thing. The Zeiss microscope I had on demo was not much better. The Nikons seem quite good ( I have three of these in use)but I have not had a research one with really good optics but might go with one of them under certain circumstances i.e. financial constraints. The Leica DMR which I use for image analysis is a pleasure to use in every way and the optics are unbeatable. Richard Black
Presentation Technologies Martin Wieczorkowski 779 Palomar Avenue Sunnyvale, Ca 94086,USA
It is 6 years ago that I tried to contact the company, so I don't know if it exists any more. I purchased the Montage in 1993 from a German Company
Raebel EDV Kolbermoorer Str. 8a D-83043 Bad Aibling Tel.: ++49-806-4015 or 4016 Fax: ++49-8061-35714 email:info-at-raebel.de WWW: http://www.raebel.de
According to their homepage they sell the Montage FR2 up to now.
Cheers, Markus
At 17:40 19/08/00 -0600, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
******************************************* Dr. Markus Drechsler Friedrich-Schiller-Universitaet Jena Institut fuer Pharmazie Lehrstuhl fuer Pharmazeutische Technologie Lessingstr.8 D-07743 Jena
Two thoughts: 1) Photograph the images with very fine grain film like Tech Pan or better. You'll lose a little from the copying, but probably less than with digitizing. The negatives can then be digitized and also archived. 2) See if someone at UE has a drum scanner -- a university print shop, or some local pre-press business. Perhaps even the Art department, if they do computer art (or not).
Phil
} I apologise if this is a little out-of-gamut for this list, but I have been } trying to make digital copies of archival material which only exists } as small prints on lustre paper. On a flat bed scanner, the textured } surface causes objectionable specular reflections which degrade } image quality. Is there a good way to avoid this? } } Chris } ========================================== } Dr. Chris Jeffree } University of Edinburgh } BIOSEM - Biological Sciences Electron Microscope Facility } Institute of Cell and Molecular Biology } Daniel Rutherford Building } King's Buildings, Mayfield Road } EDINBURGH, EH9 3JH, Scotland, UK } Tel. #44 (0) 131 650 5345 } FAX. #44 (0) 131 650 6563 } Mobile 07710 585 401 } email c.jeffree-at-ed.ac.uk } =========================================
-- }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{ Philip Oshel Supervisor, AMFSC and BBPIC Dept. of Animal Health and Biomedical Sciences University of Wisconsin 1656 Linden Drive Madison, WI 53706-1581 voice: (608) 263-4162 fax: (608) 262-7420 (dept. fax)
Hi All, Forgive me for sounding like a cheerleader, but I want to thank the LAC gang in Philadelphia for a job very well done! The meeting was a pleasure to attend. I hope everyone is able to take a well desreved breather this week.
Next year in Long Beach.
Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Responding to the message of {v03100003b5c6b7d13bc9-at-[140.251.145.24]} from Leona Cohen-Gould {lcgould-at-mail.med.cornell.edu} : } Hi All, } Forgive me for sounding like a cheerleader, but I want to thank the LAC } gang in Philadelphia for a job very well done! The meeting was a pleasure } to attend. I hope everyone is able to take a well desreved breather this } week. } } Next year in Long Beach. }
A sentiment shared by the whole program committee for the Microscopy and Microanalysis 2000 meeting!
Plan on Long Beach for MandM 2001.
__________________ Stuart McKernan stuartm-at-tc.umn.edu Office:(612) 626-7594 IT Characterization Facility, University of Minnesota Desk:(612) 624-6009 100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368
} Although I do not do confocal microscopy, I have been asked to, describe } it to new graduate students as part of a general lecture on microscopy. I } would like to present a Z-series in color along with a reconstructed } image. Can anyone point me to a source (preferably electronic) where I } might find such a thing. } } TIA } } Greg Erdos
Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program PO Box 110580 University of Florida Gainesville, FL 32611
Hello All, we have a client with an old Philips EM200. The client had a large number of Mercury Batteries stored in his Refrigerator and now the supply is used-up. Does anyone have an alternate or a supplier ? The last 2 Batteries exploded. Thank you. Peter A. Stolzenberg, PESTO Inc., Philadelphia , PA 215-699-6160 FAX 215-699-5275
Microscopy listserv readers: Hildy has asked that I relay this message to the microscopy listserv, because she no longer has access to the account through which she subscribed. --John =================
} From: Hildegard H Crowley {hcrowley-at-du.edu}
} } Dear EM Netters: } } I'm interested in purchasing sterile syringe filters to be used } to rinse my grids. I have found some that has a pore size } of 0.1 microns. They have a typical fluid retention of {= 50 } lambda. The membrane is made of hydrophilic polyethersulfone. } Has anyone used these or the filter membrane? If so, do } they require priming with preboiled deionized water? } }
Donald G. Awbrey, HT(ASCP) QIHC Electron Microscopy / Image Analysis Donald Awbrey-at-hmhs.com donaldawbrey-at-hotmail.com (817)-878-5647
Honeywell in Phoenix, Arizona is looking for an experienced SEM/EDS/WDS operator to assist in the analysis of aerospace materials. Experience also desired in metallography of aerospace materials. For more information contact Juan Trillo at 602-231-2321 or reply to juan.trillo-at-honeywell.com
Harry Ekstrom Materials Laboratory (602) 231-2744 e-mail: harry.ekstrom-at-honeywell.com
Has anybody tried to download the summary of the verbal presentation at the Philadelphia meeting on the characterization of surface topography using AFM
and SEM By Dr John Russ ? For some reason all I get is a blank page. I'm not sure if it has something to do with my internet connection.
Regards W. Erasmus
Willem Erasmus Snr. Scientist, Basic Catalysis Research Sasol Technology Tel : +27 +16 9603954 Fax : +27 +16 9602826 E-mail : willem.erasmus-at-sasol.com
Dear All, We're in the process of setting up for U-Th-Pb chemical dating of monazite, and are in need of standards for Th and Pb. A particularly useful reference in this regard is that of Scherrer et al 2000 (Schweiz. Mineral. Petrol. Mitt), which summarises all kinds of stuff. And I've a couple of questions. 1) Does anyone know how to synthesise ThP2O7. Can it be done in the same way as REE orthophosphates? 2) Does anyone have any ideas on the stability under the beam of crocoite and it's suitability as a reference standard e.g., homogeneity, zoning etc. etc. Handy hints always helpful, Malc.
-- Dr MP Roberts Phone: [+27](0)46 603 8313 Dept of Geology Fax: [+27](0)46 622 9715 Rhodes University Cell: 083 4060 262 (usually off) 6140 Grahamstown e-mail: m.roberts-at-ru.ac.za SOUTH AFRICA
In a message dated 8/22/00 2:52:57 AM, willem.erasmus-at-sasol.com writes:
} Has anybody tried to download the summary of the verbal presentation at } the Philadelphia meeting on the characterization of surface topography using } AFM and SEM By Dr John Russ ? For some reason all I get is a blank page. I'm } not sure if it has something to do with my internet connection.
The paper does seem to download OK. If you are having difficulty because your browser doesn't like the ftp:// designation, try going to {http://personal.rdu.bellsouth.net/~jcr5/index.html/} and selecting the paper from there.
I was able to successfully access the website referenced by Dr. Russ. I did notice that immediately upon arriving at the site, it opened up Adobe Acrobat. Perhaps your browser does not have that plug-in.
Hope this helps,
David A. Bell Scientist Electron Microscopy Lab Millipore Corporation 80 Ashby Road Bedford, MA 01730 (781) 533-2108
We are discarding a EM-301 within the next few days. There are many new spare parts (electronic components, mechanical parts, etc.). Please contact me directly using the email address below if you need anything.
Owen
============================= Owen P. Mills Electron Optics Facility Engineer Michigan Technological University Metallurgical & Materials Engineering Rm 512 M&M Building Houghton, MI 49931 906-487-2002 906-487-2934 (FAX) opmills-at-mtu.edu
Malcolm Roberts wrote: } 2) Does anyone have any ideas on the stability under the beam of } crocoite and it's suitability as a reference standard e.g., homogeneity, } zoning etc. etc.
Dear Malcolm,
SPI uses crocoite and galena as Pb-standards in their minerals standards for X-ray microanalysis. I take this to indicate that crocoite is a suitable choice.
Best regards, Jorgen Bilde-Sorensen
***************************** J. B. Bilde-Soerensen Senior Research Scientist, Ph. D. Materials Research Department Risoe National Laboratory DK-4000 Roskilde Denmark
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Dear Microscopists
Has anybody tried to download the summary of the verbal presentation at the Philadelphia meeting on the characterization of surface topography using AFM
and SEM By Dr John Russ ? For some reason all I get is a blank page. I'm not sure if it has something to do with my internet connection.
Regards W. Erasmus
Willem Erasmus Snr. Scientist, Basic Catalysis Research Sasol Technology Tel : +27 +16 9603954 Fax : +27 +16 9602826 E-mail : willem.erasmus-at-sasol.com
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I am looking for a new or used microtome which will work well in sectioning 0.5-5 mil polymer films and also polymer pellets. A used manual microtome would be fine. Also looking for a good bargain. Thanks!
Greetings all. I am a grad student in the psych dept at UC Berkeley. I am attempting to do HRP histochemistry on rat brain tissue. I have encountered several problems along the way. I hope someone on this list will be gracious enough to either make a suggestion or point me towards a resource which is helpful.
Briefly, my problems are these:
When I perfuse the animal via the left ventricle, frequently the lungs fill up and the brain is poorly fixed, regardless of which fixative I use. What's perplexing about this is that it seems to occur only when the fixative is added; typically the blood flush goes perfectly well. I usually don't move the 26 ga. needle when the solutions are changed. Should I be putting the needle in the aorta, as opposed to the L. ventricle? How can I do that without damaging the vessel?
I perfused a couple animals with 1% paraform/1.25% gluteraldehyde and found the brains to be mushy-- virtually unsectionable. Despite the problems with the lungs filling up & my worries about fixation, the liver appeared to be hardened, so I assumed something had happened. The glut was opened } 1 year ago, but I made the solution 48 hours before the procedure. What might have caused the brain to be so poorly fixed? Should I assume that the perfusion itself was poor, or, does the age & contact with air matter when using gluteraldehyde?
Thanks. I hope this is the appropriate list for these kinds of questions.
} Greetings all. I am a grad student in the psych dept at UC Berkeley. I am } attempting to do HRP histochemistry on rat brain tissue. I have } encountered several problems along the way. I hope someone on this list } will be gracious enough to either make a suggestion or point me towards a } resource which is helpful. } } Briefly, my problems are these: } } When I perfuse the animal via the left ventricle, frequently the lungs } fill up and the brain is poorly fixed, regardless of which } fixative I use. What's perplexing about this is that it seems to occur } only when the fixative is added; typically the blood flush goes perfectly } well. I usually don't move the 26 ga. needle when the solutions are } changed. Should I be putting the needle in the aorta, as opposed to the } L. ventricle? How can I do that without damaging the vessel? } } I perfused a couple animals with 1% paraform/1.25% gluteraldehyde and } found the brains to be mushy-- virtually unsectionable. Despite the } problems with the lungs filling up & my worries about fixation, the } liver appeared to be hardened, so I assumed something had happened. The } glut was opened } 1 year ago, but I made the solution 48 hours } before the procedure. What might have caused the brain to be so } poorly fixed? Should I assume that the perfusion itself was poor, or, does } the age & contact with air matter when using gluteraldehyde? } } Thanks. I hope this is the appropriate list for these kinds of } questions. } } Sincerely, } } bradley cooke } UC Berkeley
Bradley:
First, a 26 gauge needle is too small to deliver an adequate volume of fixative unless you are perfusing an embryo or a very young animal. However, the real problem is that you are going through the interventricular septum with the tip of the needle and perfusing the pulmonary circulation, not the systemic circulation. Thus firm lungs and mushy brain. Get a small piece of cork sheet or thin tubing. Poke the perfusion needle (20 or 18 ga.) through it so that only 2-3 mm protrudes. When entering the left vent. use the cork as a guide to prevent penetrating too far AND stay a bit to the right (your right, not the animal's) of the apex of the heart. The angle of "attack" should be about like this backslash mark. \ Also, make sure the cork does not slide on the shaft of the needle, otherwise your guide will become useless. Also, see if you can find an experienced perfuser to help you. Practice.
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
Hi Bradley, I haven't done whole animal perfusions in years, so I'll leave comments about that to others, but will say something about your fix solution. Your glutaraldehyde is OLD. It oxidizes over time and becomes pretty worthless as a fixative. My advice is to buy a fresh supply, preferably in small volumes. Many of the EM suppliers sell vials of 10 ml each of a variety of concentrations, sealed under nitrogen or other inert gas, so that you can open a fresh vial and have an easy dilution each time you need to make fix.
You probably need to leave the brain immersed in fix for a period of time after you've perfused it, but that will depend on how robust your antigen is....how much fixation will it tolerate?
I'm sure if you talk to the people in UC Berkely's animal facility, someone there should be able to help you get the perfusion set up properly. Its their job to make sure that animals are handled within guidelines, and they should be familiar with certain procedures.
Good luck, get fesh chemicals! Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Hi Bradley, I haven't done whole animal perfusions in years, so I'll leave comments about that to others, but will say something about your fix solution. Your glutaraldehyde is OLD. It oxidizes over time and becomes pretty worthless as a fixative. My advice is to buy a fresh supply, preferably in small volumes. Many of the EM suppliers sell vials of 10 ml each of a variety of concentrations, sealed under nitrogen or other inert gas, so that you can open a fresh vial and have an easy dilution each time you need to make fix.
You probably need to leave the brain immersed in fix for a period of time after you've perfused it, but that will depend on how robust your antigen is....how much fixation will it tolerate?
I'm sure if you talk to the people in UC Berkely's animal facility, someone there should be able to help you get the perfusion set up properly. Its their job to make sure that animals are handled within guidelines, and they should be familiar with certain procedures.
I agree with Geoff's suggestions. We actually use a 16 gauge gavage needle and clamp it with a hemostat. This gives us good perfusion of the nervous system. I'd like to suggest a great paper that may help you:
Andrew Fix and Robert Garman, Toxicologic Pathology, vol.28, no.1, pp. 122-131, 2000. "Practical Aspects of Neuropathology: A Technical Guide for Working with the Nervous System."
Good luck, Sandy Perkins
Laboratory for Neurotoxicity Studies VA-MD Regional College of Vet Med Virginia Tech
Once you find a useful gauge of needle, use an emery stone to reduce the bevel of the tip, or get a short stub adapter of that guage and give it a slight bevel. This will prevent accidentally slicing the heart, or your finger.
A permanent method to create an insertion stop is to mix dental acrylic and build up a bead on the needle while rotating it. Continue rotating until it hardens.
Glen --
Glen MacDonald Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 glenmac-at-u.washington.edu (206) 616-4156 (206) 616-1828 fax ************************************************************************ C:} The box said "Requires Windows95 or better". So I bought a Macintosh. ************************************************************************
Experienced Confocal Microscopy/Electron Microscopy Technologist to work in Research Laboratory of Department of Pathology
The person we seek will be responsible for organization of a new facility that includes a confocal microscope and an electron microscope. The position includes overall management of the microscopy facility, user training, and user supervision. Requirements for the position include experience with light and transmission electron microscopy. This individual will oversee all aspects of specimen accession and processing, operation of the microscopes, photography, record keeping, and supervision of a technician. Knowledge of EM, biology, and pathology, as well as photographic procedures is required. The individual will work with only minimum supervision.
Position Requirements
Experience with confocal and digital imaging techniques, microinjection, visualization of living cells containing fluorescent probes, photobleaching, and fluorescence in situ hybridization.
Minimum of five years experience working in an electron microscopy laboratory with hands-on experience in tissue processing, dark room photography, and operation and maintenance of electron microscope.
Two years experience working on electron microscopy of human tissue specimens with knowledge of histology and pathology.
Excellent interpersonal and organizational skills are essential.
BachelorUs degree in science desirable.
Desirable Experience
Expertise in training in the operation of confocal microscope systems is a distinct advantage.
Familiarity with light microscopy methods, immunofluorescent staining, use of fluorescent probes for physiologic measurements and the general principles of cell biological research are critical. Significant facility with computers is desired.
Responsibilities
Serve as the technical manager of the facility and be responsible for the operation and maintenance of the confocal and EM microscope facility.
Perform routine transmission EM, including tissue processing, ultramicrotomy, and examination; do preventative maintenance on the equipment; maintain the lab, order supplies, schedule instruments, and oversee billing.
Image analysis at the light, confocal and electron microscopic levels and preparation of micrographs for publication.
Send resume to: Suzanne Mirra, M.D., Chair Department of Pathology Box 25 SUNY-Brooklyn 450 Clarkson Av. Brooklyn, NY 11203-2098
----------------- Submitted by Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Experienced Histotechnologist to work in Neuropathology Research Laboratory of Department of Pathology
The person we seek will be responsible for maintaining a neuropathology research laboratory in the Department of Pathology at SUNY Downstate Medical Center. Knowledge of immunohistochemistry, special stains, and histopathology is required. Previous experience in handling central nervous system tissue preferred. The individual will work with only minimum supervision.
Position Requirements
Minimum of five years experience working in a laboratory with hands-on experience in tissue processing, histochemistry, immunohistochemistry, and operation of a light microscope. Experience with technical aspects of neuropathology and performing special stains including silver (Bielschowsky) and myelin stains.
Two years experience working on immunohistochemistry of CNS tissue specimens with knowledge of immunoreagents and experimental protocols.
BachelorUs degree in science desirable.
Laboratory skills including communications, ability to comply with safety and laboratory regulations, maintenance of laboratory equipment and resources, operation of computers and office equipment.
Advanced computer skills (word processing and database management) essential.
Desirable Experience
Confocal microscopy desirable.
Salary commensurate with experience
Responsibilities
Purchasing supplies and equipment, budget reports, laboratory maintenance and brain banking.
Will operate all microscopic, photographic and computer equipment, and keep accurate records of all laboratory experiments and procedures. Light and fluorescent microscopy; tissue processing for paraffin embedding, sectioning and slide stainer for immunohistochemical procedures; computer imaging (PhotoShop); general photography; and library and web searches
Familiarity with computer software for keeping records, report preparation and table/figure construction using Microsoft Office software (Word, Excel, PowerPoint) as well as Endnote and Adobe PhotoShop.
Send resume to:
Suzanne Mirra, M.D., Chair Department of Pathology Box 25 SUNY-Brooklyn 450 Clarkson Av. Brooklyn, NY 11203-2098
------------------- Submitted by Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Experienced Electron Microscopy Technologist to work in Research Laboratory of Department of Pathology
The person we seek will be responsible for the overall operation of the EM laboratory in the Department of Pathology at SUNY Downstate Medical Center. This individual will oversee all aspects of specimen accession and processing, operation of the microscope, photography, record keeping, and supervision of a technician. Knowledge of EM, biology, and pathology, as well as photographic procedures is required. The individual will work with only minimum supervision.
Position Requirements
Minimum of five years experience working in an electron microscopy laboratory with hands-on experience in tissue processing, dark room photography, and operation and maintenance of electron microscope.
Two years experience working on electron microscopy of human tissue specimens with knowledge of histology and pathology.
BachelorUs degree in science desirable.
Laboratory management skills including effective written/verbal communication skills to interact with a diverse group, ability to comply with safety and laboratory regulations, maintenance of laboratory equipment and resources, and operation of computers and office equipment.
Desirable Experience
Previous experience in confocal microscopy highly desirable.
Previous experience in performing immunocytochemical staining and advanced computer skills usage (e.g. image analysis) is also desirable.
Salary commensurate with experience
Responsibilities
Maintain electron microscope in operating condition. Process clinical and research tissues for "thick" and "thin" sectioning. Darkroom management of photographic printing.
Send resume to: Suzanne Mirra, M.D., Chair Department of Pathology Box 25 SUNY-Brooklyn 450 Clarkson Av. Brooklyn, NY 11203-2098
----------------- Submitted by: Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Does anyone have experience with processing lens from a dog eye in plastic? The lens was fixed in 10% NBF. I'm trying glycol methacrylate, using the JB-4 Plus kit. I trimmed the lens to 2-3 mm thick and infiltrated overnight. I'm just sectioning the first block. Areas of the lens are fractured. I'm thinking I need to try longer infiltration. But is there a better way? Thanks for any advice.
Marcia Pitzenberger Research Associate Merck & Co. WP45-251 West Point, PA 19438 (215) 652-9767 voice marcia_pitzenberger-at-merck.com
Just to make the "GA is old" point a little clearer: GA polymerises with time and higher temperatures; a nitrogen head does not change much. Frozen, GA will last an awfully long time and if stored refrigerated most people would still be happy with the product after six months and more. While in the lab storage should not be a problem, we can be sure that inappropriate storage is common. The problem is in transit, when during the summer months the GA could see some rather high temperatures during a truck or even an air journey. We have always taken some care with GA, but being a long way from the manufacturer we have adopted special precautions such as shipping with ice and mostly by air and incoming shipments are also packed in ice and refrigerated whenever not in the air. Its costly, but this assures a better product. The high purity Formaldehyde 16% does not require refrigeration, keeps for years and is an excellent fixative. I don't know how well this works in perfusion fixation, but it may well be a good alternative. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Wednesday, August 23, 2000 4:23 AM, "lcgould-at-mail.med.cornell.edu"-at-sparc5.microscopy.com [SMTP:"lcgould-at-mail.med.cornell.edu"-at-sparc5.microscopy.com] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Hi Bradley, } I haven't done whole animal perfusions in years, so I'll leave comments } about that to others, but will say something about your fix solution. } Your glutaraldehyde is OLD. It oxidizes over time and becomes pretty } worthless as a fixative. My advice is to buy a fresh supply, preferably in } small volumes. Many of the EM suppliers sell vials of 10 ml each of a } variety of concentrations, sealed under nitrogen or other inert gas, so } that you can open a fresh vial and have an easy dilution each time you need } to make fix. } } You probably need to leave the brain immersed in fix for a period of time } after you've perfused it, but that will depend on how robust your antigen } is....how much fixation will it tolerate? } } I'm sure if you talk to the people in UC Berkely's animal facility, someone } there should be able to help you get the perfusion set up properly. Its } their job to make sure that animals are handled within guidelines, and they } should be familiar with certain procedures. } } Good luck, get fesh chemicals! } Lee } } }
Dear Bradley, Perhaps an easier solution to the danger of penetrating the wrong heart chamber is to use an over-the-needle Teflon I.V. catheter. Your animal care personnel can procure them for you, or if you have a hospital handy, their supply warehouse will have them. You can use a 20-23 gauge catheter in a rat. The sharp needle protrudes through the lumen of the catheter; you introduce the needle, bearing the catheter, into the left ventricle, just enough to penetrate the muscle wall, then slide the Teflon catheter part over the needle, deeper into the heart. With practice, I have been able to guide the catheter into the ascending aorta. The needle is then slid backwards out of the catheter and set aside. The catheter has a female hub end that will accept IV tubing, syringe hubs or injection caps, whatever your preference. Since this is a terminal procedure, you can clean the catheter and needle and reuse them. Good luck! Missy
Eleanor M. Josephson, D.V.M., Ph.D. Assistant Professor Department of Anatomy, Physiology and Pharmacology College of Veterinary Medicine 111 Greene Hall Auburn University, AL 36849-5518 Ph. (334) 844-5423 FAX (334) 844-4542 josepem-at-vetmed.auburn.edu
During the Facilities Management session at MM2000, we discussed online sign-up for instruments. At that time I could not tell anyone the specifics of the one we use. `It is free-ware and can be found at this site:
http://www.webprog.com/appoint/freetest.html
you can see it running at this site:
http://scope.biotech.ufl.edu/index.html Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program PO Box 110580 University of Florida Gainesville, FL 32611
DEPARTMENT OF PHYSICS, UNIVERSITY OF ILLINOIS AT CHICAGO
{smaller} A postdoctoral fellow position is available at the University of Illinois at Chicago for the development and application of novel STEM/TEM techniques to the analysis of metal-oxide interfaces and grain boundaries in metals. Research in the Interface Physics Group focuses on the use of atomic resolution imaging and analytical techniques in electron microscopy, coupled with theoretical simulations, to determine the structure-property relationships at internal interfaces on the fundamental atomic scale. Other related research programs within the Interface Physics Group include ceramics, ionic conductors, high-Tc superconductors and semiconductor materials/devices, and it is expected that the successful applicant will work closely with the existing group members in these areas. =20
The experimental facilities to perform this research are comprehensive: a JEOL 2010 Field-Emission STEM/TEM featuring a 1.4=C5 probe size, "drift free" stage, high-angle annular dark-field detector (Z-contrast), Gatan Imaging Filter, Noran EDS, Gatan liquid nitrogen cooling stage, and Gatan heating stage; a VG HB601 Field-Emission dedicated STEM featuring a 2.2=C5 probe size and EDS and EELS capabilities; a JEOL 3010 conventional TEM with digital imaging capabilities and EDS; a JEOL 6320 =46ield-Emission SEM with EDS; and a JEOL JXA733 microprobe. In addition to the electron microscopes, specimen preparation facilities include a Gatan Duo-mill, Fischione precision ion-mill, SouthBay plasma cleaner and Leica Ultramicrotome. The Interface Physics Group has a Silicon Graphics R10000 Power Indigo workstation with the Molecular Simulations' Cerius 2 package incorporating the CASTEP pseudopotential code. The physics department has additional workstations and access to the UIC Convex Exemplar Supercomputer and the National Center for Supercomputing Applications at UIUC. =20
Successful candidates will be recent Ph.D. graduates in physics, metallurgy, or materials science with a sound background in the relevant materials issues and an ambition to be part of a developing program pushing at the frontiers of interface physics. Please send a resume and publication list to Professor Nigel D. Browning at the address below. Prior experience in STEM or TEM is essential. =20 However, consideration will be based on the candidates overall potential for success in the field and applicants with prior experience in related fields are encouraged to apply. Positions are for one year initially, normally renewed for a second year with possibilities existing for further years. Salary is commensurate with experience.=20 UIC is an equal opportunity employer.
Cold Spring Harbor Laboratory is seeking an experienced and responsible microscopy technician for the laboratories core microscopy facility. The individual should have practical expertise in transmission electron microscopy, confocal and widefield fluorescence microscopy, digital imaging, and microinjection. The successful candidate will be involved in designing and carrying out experimental protocols for users, training individuals in the use of various microscopes, and aligning microscopes and keeping the facility operating at an efficient and high level of productivity. Interested individuals should send their resume, including a description of their expertise and the names and addresses of 3 references to: Dr. David L. Spector, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724, email: spector-at-cshl.org
At 09:45 AM 8/23/00 -0400, Greg Erdos wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I would like to second the opinion of Greg Erdos. We have been using the same calendar program and have been quite happy with it. You can see it running at our site:
http://katie.ucdavis.edu/
then click on Schedule Time.
Rick A. Harris, Director Microscopy and Imaging Facility Section of Molecular and Cellular Biology 1241 Life Sciences Addition University of California Davis, CA 530 752 2914 530 754 7536 fax http://katie.ucdavis.edu raharris-at-ucdavis.edu
I would like to try to visualize ice in frozen plant leaves in a Hitachi 2460 variable pressure SEM and do not have access to a cold stage. I have heard that a block of aluminum or copper at LN temperature may make a usable substitute. Has anyone had any experience with this technique? Any and all tips would be appreciated.
TIA
Bob Dr. Robert R. Wise Associate Professor of Plant Physiology Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (920) 424-3404 wise-at-uwosh.edu http://www.uwosh.edu/departments/biology/wise/wise.html
We're considering putting a digital camera on our JEOL 1200EX STEM. Three possibles are from Gatan, AMT, and SIS. Does anyone care to comment (positively or negatively) about retrofitting an older instrument with a camera system, and recommend one system over the other?
I have seen the results of a Gatan Megaview on an older Philips 420 which was rather impressive. I have an older AMT on a 2000FX that works quite well. I saw the SIS system at M&M. I think that you will be pleased with whatever system that you buy. You may still need to use film occasionly, but the bulk of your work will use the camera. -Scott
-----Original Message----- } From: "Beverly_E_Maleeff-at-sbphrd.com"-at-sparc5.microscopy.com [mailto:"Beverly_E_Maleeff-at-sbphrd.com"-at-sparc5.microscopy.com] Sent: Wednesday, August 23, 2000 4:15 PM To: Microscopy-at-sparc5.microscopy.com
Colleagues:
We're considering putting a digital camera on our JEOL 1200EX STEM. Three possibles are from Gatan, AMT, and SIS. Does anyone care to comment (positively or negatively) about retrofitting an older instrument with a camera system, and recommend one system over the other?
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I did see this done by an application engineer when he was demonstrating a VP SEM. We really wanted to look at hydrated tissue and the only way was to freeze a metal specimen mount in liquid nitrogen and then put the sample on it as quiackly as possible. Then put it in the scope and, with the pressure as high as possible, let the vacuum "freeze-dry" the sample. If you were just wanting a quick look at hydrated material, this works fairly well. If you really want to do reproducible work with the frozen plant material, you really need a true cold stage. We do have a cryo stage and have used it for similar projects as well as for a whole host of other samples. It really is a very valuable accessory to our SEM.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
On Wednesday, August 23, 2000, Bob Wise {wise-at-vaxa.cis.uwosh.edu} wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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-----Original Message----- } From: tgc [mailto:tgc-at-gatan.com] Sent: Wednesday, August 23, 2000 3:43 PM To: microscopy-at-msa.microscopy.com
Hi Bob, We use the frozen block technique with a Hitachi 2250N (earlier model than yours, also fitted with Peltier and ln2-cooled stages) quite often for quick observations. It works well, but you only have a limited time (few minutes) before the ice sublimes, followed by 15-30 minutes of good stable viewing of the freeze-drying sample - if you want to examine ice distribution in situ with any accuracy at all, I dont think there is any alternative to a controllable ln2-cooled stage. Working nearer room temp with a Peltier stage at higher chamber water pressures probably wouldnt help very much either, since you would be balancing condensation/sublimation...
good luck!
Sally Stowe
Dr Sally Stowe Facility Coordinator, ANU EMU Box 475 Canberra ACT 2601 AUSTRALIA stowe-at-rsbs.anu.edu.au fax 61 (0)2 6249 3218 or 6279 8525
} } } Bob Wise {wise-at-vaxa.cis.uwosh.edu} 08/24/00 03:09am } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I would like to try to visualize ice in frozen plant leaves in a Hitachi 2460 variable pressure SEM and do not have access to a cold stage. I have heard that a block of aluminum or copper at LN temperature may make a usable substitute. Has anyone had any experience with this technique? Any and all tips would be appreciated.
TIA
Bob Dr. Robert R. Wise Associate Professor of Plant Physiology Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (920) 424-3404 wise-at-uwosh.edu http://www.uwosh.edu/departments/biology/wise/wise.html
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Microscopy Core Technician.
Cold Spring Harbor Laboratory is seeking an experienced and responsible microscopy technician for the laboratories core microscopy facility. The individual should have practical expertise in transmission electron microscopy, confocal and widefield fluorescence microscopy, digital imaging, and microinjection. The successful candidate will be involved in designing and carrying out experimental protocols for users, training individuals in the use of various microscopes, and aligning microscopes and keeping the facility operating at an efficient and high level of productivity. Interested individuals should send their resume, including a description of their expertise and the names and addresses of 3 references to: Dr. David L. Spector, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724, email: spector-at-cshl.org
I to was very impressed with the AMT, SIS, and Gatan cameras at the M&M 2000. I have a 1986 Hitachi 7000 STEM and would like to know if any one out there has any digital experience with this make and model. Thanks, Tim
Timothy G. Schneider Director of Electron Microscopy Department of Pathology Room 229 Jefferson Hall Thomas Jefferson University 1020 Locust St. Philadelphia Pa. 19107 215-503-4798 work 610-613-8170 cellular timothy.schneider-at-mail.tju.edu
I am looking for a introductory technical reference for the Focused Ion Beam (FIB) technique. A journal article or textbook section would be useful. Can you suggest a comprehensive source?
After consulting with Dr. Garber, we are in general agreement on his position concerning shipping and storing of GA. We believe adding cold shipping requirements are overkill, but at the same token if any of our customers feel it is necessary we are glad to oblige. We have a long history of producing highly purified GA and in forty some years have never had a problem with our guarantee of one year at RT. There are conditions that could merit special packaging and shipping and we would be glad to conform to any requests, but webelieve it to be an unnecessary expense for most people.
John Arnott Chairman
--
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net
ALL NEW WEB SITE UP NOW AT OUR NEW URL:
http://www.laddresearch.com
Microscope Supplies Since 1955
} } Jim Darley wrote: } } ============================================================= } } Just to make the "GA is old" point a little clearer: } } GA polymerises with time and higher temperatures; a nitrogen head does not } } change much. Frozen, GA will last an awfully long time and if stored } } refrigerated most people would still be happy with the product after six } } months } } and more. While in the lab storage should not be a problem, we can be sure } } that } } inappropriate storage is common. } } The problem is in transit, when during the summer months the GA could see } } some } } rather high temperatures during a truck or even an air journey. We have } } always } } taken some care with GA, but being a long way from the manufacturer we have } } adopted special precautions such as shipping with ice and mostly by air and } } incoming shipments are also packed in ice and refrigerated whenever not in } } the } } air. Its costly, but this assures a better product. } } (snip) } } ==============================================================
Jim Darley wrote: ============================================================= Just to make the "GA is old" point a little clearer: GA polymerises with time and higher temperatures; a nitrogen head does not change much. Frozen, GA will last an awfully long time and if stored refrigerated most people would still be happy with the product after six months and more. While in the lab storage should not be a problem, we can be sure that inappropriate storage is common. The problem is in transit, when during the summer months the GA could see some rather high temperatures during a truck or even an air journey. We have always taken some care with GA, but being a long way from the manufacturer we have adopted special precautions such as shipping with ice and mostly by air and incoming shipments are also packed in ice and refrigerated whenever not in the air. Its costly, but this assures a better product. (snip) ============================================================== Sometimes good technical people have honest differences of opinion. I am sure this is one of those instances.
I have always been led to believe that the mechanism of ageing of GA is related to the formation first of dimers and trimers of the monomer (it is an autocatalytic reaction), and that the way to ensure longest shelf life is to bring the starting purity down to levels (actually lower than I believe most EM users would need) such that one can really delay (but not indefinitely) the onset of the dimer/trimer reaction from starting. By removing the existing dimers and trimers, you remove that which starts the aging process.
When GA is purified to these levels, one can delay the onset of the aging process to such a degree, that some producers of EM glutaraldehyde guarantee their product to be good for one year at room temperature storage. SPI is one of those and as of today, I reconfirmed that the Ladd glutaraldehyde is in this category as well. Quite possibly, if not probably, there are other vendors making EM glut at that same level of purity.
But with glutaraldehyde being purified to these levels, and with these guarantees from the manufacturer for one year at room temperature, to require cold shipment in ice, or even the use of frozen gel packs (which usually are cheaper) in our opinion, is normally not required. Naturally, upon arrival, we do recommend refrigerated storage, just out of general principles, which might be more the result of "dogma" than real reasons. But we ship regularly the ampouled GA to tropical environments, unrefrigerated, and don't have any particular problems. We would discourage shipment to tropical environments via air freight, since it could end up sitting in customs for days on end but use of the courier services seems to result in no difficulties.
Now for GA being sold in screw top bottles, this would be another story. If the starting purity is good as indicated above, then refrigerated shipment should not, in general be necessary. However, we would strongly recommend the refrigerated storage upon arrival, indeed we think this should be a requirement. The guarantees for room temperature storage for one year do not extend to the product sold in the screw top bottles, only in the sealed glass ampoules. We don't know about frozen storage, but usually most users want their product in ready-to-use form and don't want to wait for something to thaw out before using.
Again, different people have different opinions about these matters, and the purpose of this posting is only to point out that there are these differences, and that we do not believe one needs to regularly incur the extra costs that would otherwise be necessary for the refrigerated shipment of GA.
Chuck
Disclaimer: SPI Supplies offers EM grade glutaraldehyde to EM laboratories worldwide and we do not refrigerate our shipments of GA and to my knowledge, we have never had a customer receive GA that has gone bad in shipment.
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
Someone has asked what resin to use that would be clear and suitable for embedding mouse embryos. This would be for display, not for sectioning.
Mahalo! Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
I have never heard of those dimmers and trimmers, just seems a change from various polymers and co-polymers. More importantly, I do not believe that Chuck's supplier has something better than vacuum distillation available to achieve highest purity. Not that I would want to get into the game of "mine is purer than yours", since I have no doubt that at production either of the three (to my knowledge) North American "refiners" produce the very best GA possible and they are comparable. The main points of this message are that: 1 For infusion and I understand cyto or immunocytochemistry the purest possible GA should be used. I have read the suggestion that a small amount of polymer may be good during conventional fixation, as this would impede osmotic shock. 2 I certainly do not agree with Chucks suggestions that GA may be stored at room temperature for a year and then used for EM fixation - regardless of initial purity. GA is not in this regard to be confused with the high purity Formaldehyde (16%), which does not require refrigeration, keeps for years and is an excellent fixative. That item come after Chuck's "snip". Hmmm. 3 The point of cold shipping that I had made was particularly pertinent to our situation were the material needs to travel a long distance. But especially during our summer months (and the USA can be just as hot) I believe that shipping GA with some ice is worth the extra cost. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Friday, August 25, 2000 9:08 AM, Garber, Charles A. [SMTP:cgarber-at-2spi.com] wrote: } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } Jim Darley wrote: } ============================================================= } Just to make the "GA is old" point a little clearer: } GA polymerises with time and higher temperatures; a nitrogen head does not } change much. Frozen, GA will last an awfully long time and if stored } refrigerated most people would still be happy with the product after six } months } and more. While in the lab storage should not be a problem, we can be sure } that } inappropriate storage is common. } The problem is in transit, when during the summer months the GA could see } some } rather high temperatures during a truck or even an air journey. We have } always } taken some care with GA, but being a long way from the manufacturer we have } adopted special precautions such as shipping with ice and mostly by air and } incoming shipments are also packed in ice and refrigerated whenever not in } the } air. Its costly, but this assures a better product. } (snip) } ============================================================== } Sometimes good technical people have honest differences of opinion. I am } sure this is one of those instances. } } I have always been led to believe that the mechanism of ageing of GA is } related to the formation first of dimers and trimers of the monomer (it is } an autocatalytic reaction), and that the way to ensure longest shelf life is } to bring the starting purity down to levels (actually lower than I believe } most EM users would need) such that one can really delay (but not } indefinitely) the onset of the dimer/trimer reaction from starting. By } removing the existing dimers and trimers, you remove that which starts the } aging process. } } When GA is purified to these levels, one can delay the onset of the aging } process to such a degree, that some producers of EM glutaraldehyde guarantee } their product to be good for one year at room temperature storage. SPI is } one of those and as of today, I reconfirmed that the Ladd glutaraldehyde is } in this category as well. Quite possibly, if not probably, there are other } vendors making EM glut at that same level of purity. } } But with glutaraldehyde being purified to these levels, and with these } guarantees from the manufacturer for one year at room temperature, to } require cold shipment in ice, or even the use of frozen gel packs (which } usually are cheaper) in our opinion, is normally not required. Naturally, } upon arrival, we do recommend refrigerated storage, just out of general } principles, which might be more the result of "dogma" than real reasons. } But we ship regularly the ampouled GA to tropical environments, } unrefrigerated, and don't have any particular problems. We would } discourage shipment to tropical environments via air freight, since it could } end up sitting in customs for days on end but use of the courier services } seems to result in no difficulties. } } Now for GA being sold in screw top bottles, this would be another story. If } the starting purity is good as indicated above, then refrigerated shipment } should not, in general be necessary. However, we would strongly recommend } the refrigerated storage upon arrival, indeed we think this should be a } requirement. The guarantees for room temperature storage for one year do } not extend to the product sold in the screw top bottles, only in the sealed } glass ampoules. We don't know about frozen storage, but usually most users } want their product in ready-to-use form and don't want to wait for something } to thaw out before using. } } Again, different people have different opinions about these matters, and the } purpose of this posting is only to point out that there are these } differences, and that we do not believe one needs to regularly incur the } extra costs that would otherwise be necessary for the refrigerated shipment } of GA. } } Chuck } } Disclaimer: SPI Supplies offers EM grade glutaraldehyde to EM laboratories } worldwide and we do not refrigerate our shipments of GA and to my knowledge, } we have never had a customer receive GA that has gone bad in shipment. } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President 1-800-2424-SPI } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.spi.cc } ######################## } ============================================ } } } } } } }
Thanks to everyone who answered my query about the Topographic Measurement paper by Dr John Russ and for all the helpful suggestions. The problem seems to have fixed itself. This morning, after about 10 more attempts, I was suddenly able to download the file. I have no idea what the problem was, and neither does our IT department. It certainly wasn't the Adobe Acrobat Reader or Internet Explorer. I have learned long ago that computers are strange things, and this just proves it again !!!
Thanks Willem Erasmus
Willem Erasmus Snr. Scientist, Basic Catalysis Research Sasol Technology Tel : +27 +16 9603954 Fax : +27 +16 9602826 E-mail : willem.erasmus-at-sasol.com
We store 25% glutaraldehyde in ampoules in the freezer compartment of a domestic fridge . It does not freeze at this temperature and is therefore ready for use very quickly.
Dave
On Thu, 24 Aug 2000 18:07:53 -0500 "Garber, Charles A." {cgarber-at-2spi.com} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } Jim Darley wrote: } ============================================================= } Just to make the "GA is old" point a little clearer: } GA polymerises with time and higher temperatures; a nitrogen head does not } change much. Frozen, GA will last an awfully long time and if stored } refrigerated most people would still be happy with the product after six } months } and more. While in the lab storage should not be a problem, we can be sure } that } inappropriate storage is common. } The problem is in transit, when during the summer months the GA could see } some } rather high temperatures during a truck or even an air journey. We have } always } taken some care with GA, but being a long way from the manufacturer we have } adopted special precautions such as shipping with ice and mostly by air and } incoming shipments are also packed in ice and refrigerated whenever not in } the } air. Its costly, but this assures a better product. } (snip) } ============================================================== } Sometimes good technical people have honest differences of opinion. I am } sure this is one of those instances. } } I have always been led to believe that the mechanism of ageing of GA is } related to the formation first of dimers and trimers of the monomer (it is } an autocatalytic reaction), and that the way to ensure longest shelf life is } to bring the starting purity down to levels (actually lower than I believe } most EM users would need) such that one can really delay (but not } indefinitely) the onset of the dimer/trimer reaction from starting. By } removing the existing dimers and trimers, you remove that which starts the } aging process. } } When GA is purified to these levels, one can delay the onset of the aging } process to such a degree, that some producers of EM glutaraldehyde guarantee } their product to be good for one year at room temperature storage. SPI is } one of those and as of today, I reconfirmed that the Ladd glutaraldehyde is } in this category as well. Quite possibly, if not probably, there are other } vendors making EM glut at that same level of purity. } } But with glutaraldehyde being purified to these levels, and with these } guarantees from the manufacturer for one year at room temperature, to } require cold shipment in ice, or even the use of frozen gel packs (which } usually are cheaper) in our opinion, is normally not required. Naturally, } upon arrival, we do recommend refrigerated storage, just out of general } principles, which might be more the result of "dogma" than real reasons. } But we ship regularly the ampouled GA to tropical environments, } unrefrigerated, and don't have any particular problems. We would } discourage shipment to tropical environments via air freight, since it could } end up sitting in customs for days on end but use of the courier services } seems to result in no difficulties. } } Now for GA being sold in screw top bottles, this would be another story. If } the starting purity is good as indicated above, then refrigerated shipment } should not, in general be necessary. However, we would strongly recommend } the refrigerated storage upon arrival, indeed we think this should be a } requirement. The guarantees for room temperature storage for one year do } not extend to the product sold in the screw top bottles, only in the sealed } glass ampoules. We don't know about frozen storage, but usually most users } want their product in ready-to-use form and don't want to wait for something } to thaw out before using. } } Again, different people have different opinions about these matters, and the } purpose of this posting is only to point out that there are these } differences, and that we do not believe one needs to regularly incur the } extra costs that would otherwise be necessary for the refrigerated shipment } of GA. } } Chuck } } Disclaimer: SPI Supplies offers EM grade glutaraldehyde to EM laboratories } worldwide and we do not refrigerate our shipments of GA and to my knowledge, } we have never had a customer receive GA that has gone bad in shipment. } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President 1-800-2424-SPI } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.spi.cc } ######################## } ============================================ } } } } } } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Is anyone familiar with the staining of silver-deposits in rat-tissues. Organs which may be involved are liver and kidneys. Is a silver-deposit within tissue possible?
Joost Bruijntjes TNO Nutrition and Food Research Zeist Holland
Exciting Opportunity for an Experienced Trasmission Electron Microscopist in Advanced Imaging and Measurement, Unilever Research, Edgewater, NJ
Position Requirements:
A full time position is immediately available for a person experienced in transmission electron microscopy of biological samples. This person will be responsible for all aspects of tissue processing, sectioning, TEM image acquisition as well as general laboratory maintenance. At least a bachelors degree in biology is required with five years of experience in bio-TEM. Previous experience in immuno-labeling, digital data acquisition and computer image processing is desirable.
Opportunity exists for the person to expand skills and capabilities in cryo-aplications (high pressure freezeing, cryo-ultramicrotomy, freze substitution/fracture etc) and environmental scanning electron microscopy.
Unilever is a leading consumer products company with world wide sales exceeding $40 billion. To support our global business, Unilever has six international research center employing over 4000 scientists. Unilever's U.S. laboratory employs over 300 scientists and is the designated headquarters for leading personal wash and skin care research. We are located on the Hudson River in Edgewater, New Jersey, within 20 minutes of midtown Manhattan with its diverse entertainment, arts and cultural resources. We offer a competitive salary, flexible benefits and excellent opportunities for both personal and professional growth. For more information about Unilever Research and Unilever visit our Web Site at http://www.unilever.com
If interested, please send resume to:
Manoj Misra Ph.D. Group Head Advanced Imaging and Measurement Unilever Research Edgewater, NJ 07020 E Mail: manoj.misra-at-unilever.com
What influence does the concentration of glutaraldehyde have on its shelf life? In the 'frig or at room temperature.
I've always thought that the higher % glut -- especially 70% -- was more likely to polymerize, and that 25% or lower had the longest shelf life. True or false?
I am assuming glutaraldehyde stored under nitrogen in sealed ampoules.
Phil
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } Jim Darley wrote: } ============================================================= } Just to make the "GA is old" point a little clearer: } GA polymerises with time and higher temperatures; a nitrogen head does not } change much. Frozen, GA will last an awfully long time and if stored } refrigerated most people would still be happy with the product after six } months } and more. While in the lab storage should not be a problem, we can be sure } that } inappropriate storage is common. } The problem is in transit, when during the summer months the GA could see } some } rather high temperatures during a truck or even an air journey. We have } always } taken some care with GA, but being a long way from the manufacturer we have } adopted special precautions such as shipping with ice and mostly by air and } incoming shipments are also packed in ice and refrigerated whenever not in } the } air. Its costly, but this assures a better product. } (snip) } ============================================================== } Sometimes good technical people have honest differences of opinion. I am } sure this is one of those instances. } } I have always been led to believe that the mechanism of ageing of GA is } related to the formation first of dimers and trimers of the monomer (it is } an autocatalytic reaction), and that the way to ensure longest shelf life is } to bring the starting purity down to levels (actually lower than I believe } most EM users would need) such that one can really delay (but not } indefinitely) the onset of the dimer/trimer reaction from starting. By } removing the existing dimers and trimers, you remove that which starts the } aging process. } } When GA is purified to these levels, one can delay the onset of the aging } process to such a degree, that some producers of EM glutaraldehyde guarantee } their product to be good for one year at room temperature storage. SPI is } one of those and as of today, I reconfirmed that the Ladd glutaraldehyde is } in this category as well. Quite possibly, if not probably, there are other } vendors making EM glut at that same level of purity. } } But with glutaraldehyde being purified to these levels, and with these } guarantees from the manufacturer for one year at room temperature, to } require cold shipment in ice, or even the use of frozen gel packs (which } usually are cheaper) in our opinion, is normally not required. Naturally, } upon arrival, we do recommend refrigerated storage, just out of general } principles, which might be more the result of "dogma" than real reasons. } But we ship regularly the ampouled GA to tropical environments, } unrefrigerated, and don't have any particular problems. We would } discourage shipment to tropical environments via air freight, since it could } end up sitting in customs for days on end but use of the courier services } seems to result in no difficulties. } } Now for GA being sold in screw top bottles, this would be another story. If } the starting purity is good as indicated above, then refrigerated shipment } should not, in general be necessary. However, we would strongly recommend } the refrigerated storage upon arrival, indeed we think this should be a } requirement. The guarantees for room temperature storage for one year do } not extend to the product sold in the screw top bottles, only in the sealed } glass ampoules. We don't know about frozen storage, but usually most users } want their product in ready-to-use form and don't want to wait for something } to thaw out before using. } } Again, different people have different opinions about these matters, and the } purpose of this posting is only to point out that there are these } differences, and that we do not believe one needs to regularly incur the } extra costs that would otherwise be necessary for the refrigerated shipment } of GA. } } Chuck } } Disclaimer: SPI Supplies offers EM grade glutaraldehyde to EM laboratories } worldwide and we do not refrigerate our shipments of GA and to my knowledge, } we have never had a customer receive GA that has gone bad in shipment. } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President 1-800-2424-SPI } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.spi.cc } ######################## } ============================================
-- }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{ Philip Oshel Supervisor, AMFSC and BBPIC Dept. of Animal Health and Biomedical Sciences University of Wisconsin 1656 Linden Drive Madison, WI 53706-1581 voice: (608) 263-4162 fax: (608) 262-7420 (dept. fax)
Another 2 cents worth. I have sat back and read with enjoyment all of the bantering regarding the facts and fiction on Glut and cannot believe that some people are actually arguing about issues which are so well cited in the literature and this argument is going to lead to the confusion of many researchers with no basis at all. The truth on Glut is short and simple and I do not care who is making it the facts remain the same no matter. Please see the facts below. Anyone whom would like to question these results I remain at your disposal to open my lab notebooks and results of all of the Glutaraldehyde in the industry today and its corresponding results. There are only 3 manufacturers of the final distilled product in the US and each and every one of them shows the same tendency towards temp vs time.
GLUTARALDEHYDE STORAGE ISSUES
A key detail in storage analysis for this product is that EM grade GA is usually supplied as "aqueous solution" of various concentrations and not as "pure", and the "pure iron won't rust" principle simply does not apply. This aqueous chemical system is well known to be extremely active, especially at temperatures exceeding +40 C (because of common chemical knowledge reasons, pertaining to water molecules intrinsic association tendency, on one hand, and to the particular GA_dimer polarisation of hydrating aggregates, on the other hand). As many EM users already know, GA polimerizes under the influence of traces of impurities, the process belonging to singlet-triplet activation mechanism (i.e., temperature, pH, concentration highly sensitive), and by no means is it an "autocatalytic" one. Traces of water (along with acrolein, ethanol, methanol, pyranic compounds), and a slight azeotropic tendency, lead ultimately to the formation of glutaric acid and consequently to the drastic decrease of pH, making GA improper for EM usage, as in: Hayat, M.A., "Principles And Techniques of Electron Microscopy", 2000. In those new created conditions, GA quickly reversibly turns to several polymeric species, some of them obviously detectable, some not. Alkaline artificially buffered solutions cannot prevent polymerisation either, and the process starts with a GA_trimer, as in: Fahimi, HD., et.al., "Essais de standardisation de la fixation en glutaralgehyde", J. Microsc. (Paris, France), 4: 725-736, 1965 (!!!) In time, several important kinetic stability studies were performed upon EM grade GA. For general information, and, for most of readers, as a kind reminder, we had selected conclusions made public in Histochemie, 30, 1972 (!!!), by Gillet, R., et.al., from Queen Elizabeth College, London, England: "if the absorbance"..."is plotted against storage temperature, a direct relationship is obtained between storage temperature (above 00 C) and the appearance of"... spectral ... "absorbing material".
(Please see graph attached.)
The authors wrote: "the most important storage criterion was definitely temperature. Those samples stored at -200 C showed virtually no change in"..."absorbance characteristics, even after eight months storage" ..."all samples stored at +200 C, however, showed considerable absorbance peaks"..."the effect of storage at +370 C"..."was even more pronounced." It appears very speculative and pragmatic to neglect such important facts, or categorize them as "dogmas". Certain manufacturers, who did not receive any complaints (yet) when delivering room temperature stored GA, should consider themselves extremely lucky, and should scrupulously revise their knowledge about EMS grade GA, regardless if sold in ampoules or screw cap bottles.
I look forward to hearing from anyone whom does not agree.
All of the very best Stacie Kirsch Electron Microscopy Sciences 215-646-1566
Please advice - I desperately need a source of info for comparative analysis of main existing microscope types - standard transmittance microscope,phase-contrast, interference, fluorescent, confocal, laser scanning ...in terms of their baic parameters: spatial resolution, sensitivity to light absorption, temporal resolution. Please advice a web-source or some other on-line esource if possible - in case of book/article I will not have time for ordering.
Thanks
Dmitri Lapotko,
Luikov Heat and Mass Transfer Institute Minsk Ld-at-hmti.ac.by
Not sure if this is the right forum to help me with this issue but thought I would give it a try. I have a student who wants to cut bone (non fossil) samples. She is not necessarily making thin sections but wants a cut better than can be done with a coping saw. I offered my Buehler Isomet low speed saw with a diamond cut off blade but that seem to be a bit too slow and awkward with the larger bones. Looking for ideas.
Thanks
Roy Beavers Southern Methodist University Dept. of Geological Sciences Electron Microprobe Lab P.O. Box 750395 Dallas, Tx 75275 voice: 214-768-2756 fax: 214-768-2701 E-mail: rbeavers-at-mail.smu.edu
Being an old timer, I have been around since before the advent of vials of GA. In the 70's the Merck index described a method for purifying GA. using activated charcoal.
Basically GA begins to degrade to "multimers" when stored at room temperature. The best fixation is achieved with the monomeric form, therefore the purification protocol was designed to remove the more complex forms.
Storage under nitrogen in a sealed vial slows the degradation. Lower concentration degrade more slowly. Storing at 4C is advised.
Method: Mix the concentrated liquid glutaraldehyde (25%) with 1g of activated charcoal per 100 ml of liquid. Allow to stand for 30 minutes, then vacuum filter through a Whatman #1 filter. Repeat three times. Store at 4C Repeat once a year.
I have used this method for more than 30 years and consistently obtained very satisfactory fixation. It is messy, but worth the effort.
I am helping, or trying to help, a colleague with sectioning of Drosophila legs. They are embedded in "Immunobed" (a methycrylate) or Epon (ok, Epon substitute). I don't seem to be getting a good bond between the chitin and the plastic, the tissue falls out. Any suggestions are welcome.
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
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Hello,
We inherited an old LKB grid stainer that has worked well for nearly two years. Now it has developed a leak.
Is there anyone who has one of these machines that they keep for spares (or to hold the door open)? I need the spindle unit for one of the pump/valve assemblies. The housing looks fine so it should be easy enough to plug in a spare unit.
For anyone who has not seen inside this machine, the above description must seem very strange. My apologies for this. My hope is that someone who has seen inside can understand my description and has the part I need.
I have already contacted Leica to see if they are still supporting this machine, so there is no need to recommend I contact them.
Thanks in advance for any help, suggestions, comments or any other e-mail you may send my way.
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id PAA23100 for dist-Microscopy; Fri, 25 Aug 2000 15:55:24 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id PAA23097 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 25 Aug 2000 15:54:53 -0500 (CDT) Received: from mail1.doit.wisc.edu (mail1.doit.wisc.edu [144.92.9.40]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id PAA23090 for {Microscopy-at-sparc5.microscopy.com} ; Fri, 25 Aug 2000 15:54:42 -0500 (CDT) Received: from [144.92.10.114] by mail1.doit.wisc.edu id PAA71884 (8.9.1/50); Fri, 25 Aug 2000 15:48:56 -0500 Mime-Version: 1.0 Message-Id: {p04320421b5cc8bee034b-at-[144.92.10.114]} In-Reply-To: {E769E1FA6D4AD311B0190008C7E65014012E44C4-at-s31xe1.systems.smu.edu} References: {E769E1FA6D4AD311B0190008C7E65014012E44C4-at-s31xe1.systems.smu.edu}
Roy,
If you don't get the answer from the microscopy group, you might try Histonet: Histonet-at-Pathology.swmed.edu to subscribe, put "subscribe" no quotes in the subject line, leave the message body blank unsubscribe the same way. Spell these correctly! They use one address for everything, so "suscribe" or "subscribe" with quotes gets sent to the list as a message, not read as a command by the serverbot. They have a bunch of folks who specialize in cutting bone, calcified and decalcified. Their archives can be searched at: http://www.histosearch.com/histonet.html
Otherwise, does someone at SMU have a diamond wire saw? Those work well for slicing intact bones. There's also a double-bladed hand bone saw that makes nice slices. Someone at the SMU med school or hospital ought to have one.
Phil
} Not sure if this is the right forum to help me with this issue but thought } I would give it a try. I have a student who wants to cut bone (non fossil) } samples. She is not necessarily making thin sections but wants a cut better } than can be done with a coping saw. I offered my Buehler Isomet low speed } saw with a diamond cut off blade but that seem to be a bit too slow and } awkward with the larger bones. Looking for ideas. } } Thanks } } Roy Beavers } Southern Methodist University } Dept. of Geological Sciences } Electron Microprobe Lab } P.O. Box 750395 } Dallas, Tx 75275 } voice: 214-768-2756 } fax: 214-768-2701 } E-mail: rbeavers-at-mail.smu.edu
-- }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{ Philip Oshel Supervisor, AMFSC and BBPIC Dept. of Animal Health and Biomedical Sciences University of Wisconsin 1656 Linden Drive Madison, WI 53706-1581 voice: (608) 263-4162 fax: (608) 262-7420 (dept. fax)
If you have a machine shop cut the bone in to thin sections on a band saw sand one side smooth and super glue the bone to a disk you can mount in a lathe or a plate you can mount on a mill or grinder. Then use thin the material as thin as you want with a grinder.
Acetone will turn them loose from the plate. You can do a bunch of sections at a time on a big plate.
Embeding them before you grind them will help preserve fine structures.
Exactly how the machine shop does the work will depend on the tools they have available.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 - } Not sure if this is the right forum to help me with this issue but thought } I would give it a try. I have a student who wants to cut bone (non fossil) } samples. She is not necessarily making thin sections but wants a cut better } than can be done with a coping saw. I offered my Buehler Isomet low speed } saw with a diamond cut off blade but that seem to be a bit too slow and } awkward with the larger bones. Looking for ideas.
I too purified GA by charcoal filtering and even vacuum distillation; its over 25 years ago. As things were published I also played around with making thin film apertures and I know that other people have made their own filaments and even the making of a LaB6 is published in the literature, for the intrepid. Don't think that I worry about loosing some business either, that too is not one of my traits. I like to make the following observations on purifying GA, we keep cathodes for another day: Charcoal filtered GA is not as good the commercial vacuum distilled product. You should store it in a freezer (even a domestic fridge's') if its for more then a few weeks. The triple charcoal filtered product is good enough for EM fixation, if stored well. It's dubious that its good enough for cytochemistry. Charcoal is a recognized carcinogen (funny that, it used to be prescribed against diarrhea) its also expensive and a DG to ship. Several ml of GA are lost in every filtration. Any employer would cost an employee at at least 2x the hourly cost; in research its easy to forget the real world, were time is money. "Home filtered" GA is very expensive.
Different parameters may apply elsewhere, so in countries were the hourly wage is 50 cents or less, and a drum of GA fell of a truck at a leather tanning or paper towel factory (its used to soften leather and paper) and somebody is making coconut charcoal down the road . . . I would be into that and make my own purified GA too. But not in America! Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Saturday, August 26, 2000 5:35 AM, L R MELSEN [SMTP:lmelsen-at-emory.edu] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Being an old timer, I have been around since before the advent of vials } of GA. In the 70's the Merck index described a method for purifying GA. } using activated charcoal. } } Basically GA begins to degrade to "multimers" when stored at room } temperature. The best fixation is achieved with the monomeric form, } therefore the purification protocol was designed to remove the more } complex forms. } } Storage under nitrogen in a sealed vial slows the degradation. Lower } concentration degrade more slowly. Storing at 4C is advised. } } Method: } Mix the concentrated liquid glutaraldehyde (25%) with 1g of activated } charcoal per 100 ml of liquid. } Allow to stand for 30 minutes, then vacuum filter through a Whatman #1 } filter. } Repeat three times. } Store at 4C } Repeat once a year. } } I have used this method for more than 30 years and consistently obtained } very satisfactory fixation. It is messy, but worth the effort. }
..We have decided as a department to close down our transmission EM } } operation. We have a Philips EM 300 ... which we are willing to donate to someone for parts, provided they pay for disassembly and shipping to their site...
For details please contact: } } } David Kerk, Ph.D. } } } Professor and Chair } } } Department of Biology } } } Point Loma Nazarene University } } } 3900 Lomaland Dr } } } San Diego, CA 92106 } } } Ph: 619-849-2398 } } } FAX: 619-849-2598 } } } email: dkerk-at-ptloma.edu
M. Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories University of California at San Diego address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368 phone: 8588223373 fax: 8588223715 pager: 8586161420 e.mail: mmm-at-ucsd.edu e.pager: 1620024619-at-alphapage.airtouch.com www site: http://mil.ucsd.edu/ ftp site: mil1.ucsd.edu
As an alternative to charcoal purification of GA described by Dr. MELSEN, I run distillation, collect the fractions, and then select the monomeric form on the spec.
} Date: Fri, 25 Aug 2000 15:35:12 -0400 } From: L R MELSEN {lmelsen-at-emory.edu} } Reply-To: lmelsen-at-emory.edu } Organization: Emory University } X-Mailer: Mozilla 4.73 (Macintosh; I; PPC) } X-Accept-Language: en } To: MICROSCOPY {Microscopy-at-sparc5.microscopy.com} } Subject: glutaraldehyde } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
M. Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories University of California at San Diego address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368 phone: 8588223373 fax: 8588223715 pager: 8586161420 e.mail: mmm-at-ucsd.edu e.pager: 1620024619-at-alphapage.airtouch.com www site: http://mil.ucsd.edu/ ftp site: mil1.ucsd.edu
} Someone has asked what resin to use that would be clear and suitable for } embedding mouse embryos. This would be for display, not for sectioning. }
Dear Tina, How about lucite? I have no idea where to get it, but many years ago I bought a lucite cube in which a dandilion had been embedded. You could see it very clearly, and there is obviously a technique for preserving such a fragile specimen. Note that this is more along the lines of an existance theorem than a procedure. Good luck. Yours, Bill Tivol
From root Mon Aug 28 13:15:45 2000 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id MAA27167 for dist-Microscopy; Mon, 28 Aug 2000 12:53:55 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id MAA27163 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Mon, 28 Aug 2000 12:53:25 -0500 (CDT) Received: from sphmgaab.compuserve.com (hs-img-2.compuserve.com [149.174.177.151]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id MAA27136 for {microscopy-at-sparc5.microscopy.com} ; Mon, 28 Aug 2000 12:34:26 -0500 (CDT) Received: (from mailgate-at-localhost) by sphmgaab.compuserve.com (8.9.3/8.9.3/SUN-1.9) id NAA20369; Mon, 28 Aug 2000 13:28:12 -0400 (EDT)
Tina:
The best material to use in display type applications is a polyester material. We have a material called PolyMet which is ideal. It takes a while to cure - typically overnight - but it provides a crystal clear mount. I hope this helps.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by Tina Carvalho } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues-
Someone has asked what resin to use that would be clear and suitable for embedding mouse embryos. This would be for display, not for sectioning.
Mahalo! Tina
*************************************************************************** * * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* *************************************************************************** *
Based on recent communications it appears there is a difference of opinion on the shipping of refined Glut among the three major U.S. refiners.
Ladd and a second refiner who has not been heard from do not add ice. The third refiner does. When adding extras there is a higher cost and, though we don't always acknowledge it, these costs do get passed on to the consumer.
All three refiners know their specific product and their techniques and make their decisions on that basis. If the refiner who adds ice feels their product requires it I have great respect for that decision. They know their product and know the best way to get it to their customer.
All data and history indicates Ladd glut does not require ice. If special circumstances arise we would be glad to add ice.
We all act in good faith. If the refiner who adds ice is right and the others are wrong I will acknowledge and applaud that decision. We do add cold packaging for other chemicals like Mercox and LR White when required.
John Arnott --
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net
Hello, One day someone said that microwave oven is very dangerous to be operated in a level with the operator. Any advise, article in this matter? Thanks Mansour Al-Shafei Senior Lab Sci. Saudi Aramco Oil Company Lab Research & Development Center Analytical Sciences Division Advanced Instruments Unit Voice: 966-3-876-4360 E-mail: shafeima-at-aramco.com.sa
} Someone has asked what resin to use that would be clear and suitable for } embedding mouse embryos. This would be for display, not for sectioning. }
Dear Tina,
There is also a product called Cast N' Craft casting resin available at craft stores (Pearl, etc.) which I have had some success. You can not cast under vacuum however you get a very clear, bubble free final product. For spiders and smaller insects its been O.K but you may have difficulty with any large objects. It's only a few bucks and may be worth a shot [provided you have a few embryos to spare :)]. Good Luck.
Regards,
George R. Munzing Jr.
William Tivol {tivol-at-wadsworth.org} -at-wadsworth.org on 08/28/2000 11:42:03 AM
Sent by: tivol-at-wadsworth.org
To: microscopy-at-sparc5.microscopy.com cc:
Tina Carvalho wrote:
} Someone has asked what resin to use that would be clear and suitable for } embedding mouse embryos. This would be for display, not for sectioning. }
Dear Tina, How about lucite? I have no idea where to get it, but many years ago I bought a lucite cube in which a dandilion had been embedded. You could see it very clearly, and there is obviously a technique for preserving such a fragile specimen. Note that this is more along the lines of an existance theorem than a procedure. Good luck. Yours, Bill Tivol
{html} {font face="Times New Roman, Times"} A postdoctoral scientist is sought for a joint research program between the University of Virginia and the IBM Thomas J. Watson Research Center, centered upon real-time electron microscope studies of the evolution of epitaxial semiconductor {br} nanostructures. This position will be funded by an anticipated National Science Foundation award to establish a Materials Research Science and Engineering Center (MRSEC) at the University of Virginia. The successful applicant will be an employee of the Department of Materials Science and Engineering at the University of Virginia, but will spend the bulk of his / her time based at IBM, using the unique in-situ imaging and {br} deposition tools in the Nanoscale Materials Analysis Department in the Physical Sciences Division at the Thomas J. Watson Research Center. {br} {br} Applicants will require a PhD (or equivalent) in Materials Science, Physics or a related discipline. The successful candidate will be able to demonstrate strong background in electron microscopy, and will preferably have expertise in ultra-high vacuum techniques. A research background in electronic materials will also be a strong asset. {br} {br} Review of applications will begin on Sep 15, and will continue until the position is filled. The starting date will be in the October November 2000 timeframe. The initial appointment will be for one year, and will be extendible annually for an additional two years, depending upon continuing funding. Applications should be addressed to: Professor Robert Hull, Department of Materials Science and Engineering, University of Virginia, 116 Engineers Way, Charlottesville, Virginia 22904. Please provide a curriculum vitae, copies of up to five recent relevant publications, and contact information for three referees familiar with your work and accomplishments. {br} {br} Additional information on the Department of Materials Science and Engineering may be found at {a href="http://www.mse.virginia.edu/" eudora="autourl"} http://www.mse.virginia.edu {/a} , and on the IBM Thomas J. Watson Research Center at {a href="http://www.research.ibm.com/" eudora="autourl"} www.research.ibm.com {/a} . The University of Virginia and IBM are Equal Opportunity / Affirmative Action employers. Applicants must be able to lawfully accept employment in the United States. {br} {br} {/font} {BR} {br} {div} Robert Hull {/div} {div} Department of Materials Science and Engineering {/div} {div} University of Virginia {/div} {div} 116 Engineers Way {/div} {div} Charlottesville, VA 22904-4745 {/div} {br} {div} Tel: 804-982-5658 {/div} {div} FAX: 804-982-5660 {/div} email:hull-at-virginia.edu {/html}
Modern microwave ovens are so well shielded that you cannot get a reading with the sensitive meters that we supply. The only way you can show that the meter is working is to place it within the microwave - for a few seconds. I'd rather worry about gamma rays from outer space. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Tuesday, August 29, 2000 9:53 PM, Shafei, Mansour A [SMTP:shafeima-at-aramco.com.sa] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, } One day someone said that microwave oven is very dangerous to be operated in } a level with the operator. Any advise, article in this matter? } Thanks } Mansour Al-Shafei } Senior Lab Sci. } Saudi Aramco Oil Company } Lab Research & Development Center } Analytical Sciences Division } Advanced Instruments Unit } Voice: 966-3-876-4360 } E-mail: shafeima-at-aramco.com.sa } }
I am seeking advice on freeze-fracture EM. I am particularly interested in bacterial membranes and cell walls, and those who do immunolocalization on these kinds of preparations.
Please contact me OFF LIST at: rjpalmer-at-dir.nidcr.nih.gov Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 308 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax 301-402-0396
We have been looking at the use of various catalysts for production carbon nanotubes. Recently we had a sample that behaved in a very unusual way. Upon focusing the electron beam on a cluster of nanotubes, the catalyst particles became fluid and ran through the tubes at about 50-100 nm/sec. I was a little surprised by the movement. The catalyst particles would stop moving within 2-5 seconds.
I have found one reference, Buffat and Borel, Phys. Rev A13, 2287(1976), that showed the melting temperature of gold nanoparticles could be reduced by 600C! for nanoparticles less than 5 nm.
In our case we have cobalt nanoparticles less than 10 nm.
My question is how high a temperature might we expect to heat the sample in the electron beam with the following parameters;
Have others seen this phenomenon? David R. Hull NASA Glenn Research Center at Lewis Field Advanced Metallics Branch Mail Stop 49-1 21000 Brookpark Road Cleveland, OH 44135
A full time position for an experienced electron microscopist is available in the laboratory of Yishi Jin at the University of California, Santa Cruz. Dr. Jin studies the cytoskeletal architecture at synapses in the nervous system of the nematode C. elegans. For more detailed information about the work being done in the lab see http://www.biology.ucsc.edu/faculty/jin.html, or consult these recent publications; Zhen et al.2000.Neuron 26:331-343 or Zhen and Jin.1999. Nature.41:371-375.
The person we seek must have prior experience in sample preparation for transmission electron microscopy of biological materials such as fixation, embedding, sectioning, staining, and photography. Proficiency in serial sectioning would be an advantage, but is not essential at the time of employment, nor is experience with C. elegans. The person selected must be highly motivated, able to work independently, and be willing to acquire new skills and techniques. A Bachelors's degree or equivalent in a biological science is desirable.
This position is funded by the Howard Hughes Medical Institute. Salary will be commensurate with experience within the range and criteria determined by HHMI.
Interested individuals should send a current CV, names, addresses, and email contact information of 3 references, and examples of previous EM work to: Dr. Yishi Jin, Department of Biology, 328 Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064 by November 1, 2000. Dr. Jin's email address is jin-at-biology.ucsc.edu.
We are still using a 15 year old Link AN10000 x-ray detector. The widths of the peaks have suddenly become much wider. The zero beam or strobe used to be around 80 eV FWHM, and now is around 360 eV, with no beam in the column. To the best of my knowledge there is no light in the column, and the dewar has plenty of LN2 in it, so I am assuming the crystal is cold. Also, if I disconnect the Lemo connector (carrying the signal from the detector) the FWHM drops to about 20 eV FWHM. If anyone has any suggestions I would be very appreciative.
Thanks,
Mick Thomas ------------------------------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
A colleague is trying to use an Olympus Fluoview CLSM System on a BX50WI upright (not inverted) microscope. She is unable to obtain a decent brightfield image. Question: Has anyone developed their own set of operating directions that they would be willing to share? Unfortunately, I have no experience with CLSM and can offer no assistance to her.
Thank you.
JB -- #################################################################### John J. Bozzola, Ph.D., Director Micro-Imaging and Analysis Center 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
I have had great successs with Castoliteto make very clear, water white mounts of various natural materials. I have seen some with dandelion heads clearly defined.
Castolite can be obtained from Buehler, Ltd. 41 Waukeegan Rd. PO box 1, Lake Bluff, IL, 60044-7979, 800-283-4537
} Tina Carvalho wrote: } } } Someone has asked what resin to use that would be clear and suitable for } } embedding mouse embryos. This would be for display, not for sectioning. } } } } Dear Tina, } How about lucite? I have no idea where to get it, but many years ago } I } bought a lucite cube in which a dandilion had been embedded. You could } see it very clearly, and there is obviously a technique for preserving } such } a fragile specimen. Note that this is more along the lines of an } existance } theorem than a procedure. Good luck. } Yours, } Bill Tivol } } } } } }
If you, or people you know, have a double tilt holder that can be used for Hitachi H7000 to sale, please contact me or Dr. Soumitra Ghoshroy (505-646-3600, sghoshro-at-nmsu.edu). TIA. ---------------------------------------------------------------------------- ------------------- Prof. Jane G. Zhu Department of Physics Phone: 505-646-1933 New Mexico State University Fax: 505-646-1934 MSC 3D, P.O. Box 30001 E-mail: jzhu-at-nmsu.edu Las Cruces, NM 88003 ---------------------------------------------------------------------------- -------------------
At the recent Microscopy and Microanalysis meeting in Philadelphia, I gave a talk entitled "The Hitachi HD-2000 In Semiconductor Manufacturing Support And Research". Based on the feed back and comments I have received, I would like to restate the goals of my talk and my opinions for all that are interested.
The HD-2000 was designed as a tool for failure mode analysis and manufacturing support. It does a wonderful job in this regard. It combines traditional SEM imaging with basic STEM operation giving SEM and TEM type capabilities in the same package. Holders are compatible with the Hitachi FIB and the rapid exchange between these tools increases the ease and success rate for manufacturing support. Use is very straightforward and requires very little training for successful and productive use. The Cirent Semiconductor/Lucent technologies primary users are extremely happy with their microscope and it has increased their capabilities in support of the semiconductor fabrication lines.
Because of its dedicated STEM design and cold field emission gun, it has potential to be a successful research level microscope for imaging and spectroscopy. In my opinion, the microscope as shipped and installed at the Lucent manufacturing site in Orlando has some key shortcomings that will inhibit its implementation at this level. However, Hitachi is currently developing improved diffraction capabilities and EELS options that should address my concerns. I look forward to seeing and evaluating their improvements.
I would be glad to discuss my opinions and impressions with anyone interested.
Richard Vanfleet vanfleet-at-physics.ucf.edu 407-823-2600
Disclaimer: I am not employed by or affiliated in any way with Hitachi.
Have you changed anything around the eds setup? Plugged a new desk lamp into a mains outlet nearby? Changed the layout of the cabling? What you describe is a pretty big jump, but it may pay to check the simple things before calling in the man.
I really messed up my PC-based system a while back by connecting up an external CD writer whose mains lead went to a socket that was on a different supply circuit, thereby producing a nice big noise-capturing earth loop.
cheers
rtch
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Fellow microscopists, } } We are still using a 15 year old Link AN10000 x-ray detector. The widths } of the peaks have suddenly become much wider. The zero beam or strobe used } to be around 80 eV FWHM, and now is around 360 eV, with no beam in the } column. To the best of my knowledge there is no light in the column, and } the dewar has plenty of LN2 in it, so I am assuming the crystal is } cold. Also, if I disconnect the Lemo connector (carrying the signal from } the detector) the FWHM drops to about 20 eV FWHM. If anyone has any } suggestions I would be very appreciative. } } Thanks, } } Mick Thomas } ------------------------------------------------- } Mick Thomas } UHV-STEM Laboratory } E-1 Clark Hall } Cornell University } Ithaca, NY 14853 } } Phone: 607-255-0650 } Fax: 607-255-7658 } e-mail: mgt3-at-ccmr.cornell.edu } } Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
How can we remove mucus from fresh / fixed tissue specimens - specifically fish / squid? A student has been recommended to try s-carboxy-methyl-L-cysteine but can find no information as to how to do this, e.g. concentration, time, temperature. Any information would be welcome about this or any other methods.
Thanks - Keith Ryan
_______________________ Keith Ryan (Dr) Marine Biological Association Citadel Hill Plymouth Devon PL1 2PB England
I wish to obtain the accesories for a TEM Philips EM201 listed bellow
Plate numbering device PW 6543/00 Automated exposure system PW 6542..
the parts can be used...
any help is welcome...
thanks in advance
Fernando =================================================== Fernando D. Balducci Laboratorio de Microscopia Electrónica Facultad de Ingeniería - Bioingenieria Universidad Nacional de Entre Rios Argentina e-mail: microsc-at-fi.uner.edu.ar tel: 54 43 975100 fax: 54 43 975077
} Date: Wed, 30 Aug 2000 11:50:24 +0100 } From: Keith Ryan {kpr-at-ccms.ac.uk} } To: Microscopy-at-sparc5.microscopy.com } Subject: SEM - mucus removal from specimens } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Greetings from Plymouth UK } } How can we remove mucus from fresh / fixed tissue specimens - } specifically fish / squid? A student has been recommended to try } s-carboxy-methyl-L-cysteine but can find no information as to how to } do this, e.g. concentration, time, temperature. Any information would } be welcome about this or any other methods. } } Thanks - Keith Ryan } } } _______________________ } Keith Ryan (Dr) } Marine Biological Association } Citadel Hill } Plymouth } Devon PL1 2PB } England } } Tel. 0044 (0)1752 633279 (with answer machine) } also 0044 (0)1752 633249 } Fax. 0044 (0)1752 633102 } } e-mail: kpr-at-wpo.nerc.ac.uk
We use Sputolysin to dissolve mucus in human lung washes. You can make it yourself with 0.1 g dithiothreitol in 100 ml water. Keep this as a stock. When ready for use, dilute 1:10 with water, and use this dilution 1:1 with sample. Let sit at room temp with periodic swirling for 30-60 min. Add more and repeat if necessary. For our procedure, it thins the mucus so that we can prepare the sample for negative staining to look for viruses.
NOW, I have no idea what is in fish slime or whether this concoction will work, but it's easy enough to try. I don't know about the cysteine and will be interested to hear what you find works.
Good luck, Sara
} }
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Keith Ryan - I can relay a little tip about this but I have not studied the chemistry in detail. A group here was doing SEM on lung and airway tissues. They were prepping the tissue for SEM by fixation, dehydration in ethanol, then using hexamethyldisilazane to dry the samples. They discovered that mucus was covering the surface of the airways. They simply added a ten minute step in xylene between the last alcohol and the HMDS. The mucus disappeared. It might be worth a try. Good luck. Sincerely,
Robert J. Munn Electron Microscopy Laboratory Department of Medical Pathology University of California, Davis School of Medicine Davis, CA 95616
Dear Mick, When I had an increase in peak width in my Kevex last year, I warmed up the detector to remove any water ice that had accumulated in the bottom of the dewar or on the crystal. Turn off the bias, short out the bias plug, pour out the liquid nitrogen, then blow hot air into the dewar until everything is warm. Unfortunately, although the resolution improved 10 eV per channel, the dewar now had poor holding time, so I had to have it re-pumped. Now, everything is great. The other problem can be related to poor grounding in the pre-amp, but that caused excessive dead-time. At 04:01 PM 8/29/00 -0700, you wrote: } } Fellow microscopists, } } We are still using a 15 year old Link AN10000 x-ray detector. The widths } of the peaks have suddenly become much wider. The zero beam or strobe used } to be around 80 eV FWHM, and now is around 360 eV, with no beam in the } column. To the best of my knowledge there is no light in the column, and } the dewar has plenty of LN2 in it, so I am assuming the crystal is } cold. Also, if I disconnect the Lemo connector (carrying the signal from } the detector) the FWHM drops to about 20 eV FWHM. If anyone has any } suggestions I would be very appreciative. } } Thanks, } } Mick Thomas Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
We remove mucus using a product used to clear mucus from airway and bowel in the clinical environment, it is Acetylcysteine, used at 1% concentration I believe. The only problem is that if there are other goblet cells in the tissue they will release immediately leading to yet more mucus..... It works though Simon
--------------------------- Simon C. Watkins Ph.D. MRC Path Associate Professor, Cell Biology and Physiology Director: Center for Biologic Imaging BSTS 225 University of Pittsburgh Pittsburgh PA 15261 Tel:412-648-3051 Fax:412-648-8330 http://sbic6.sbic.pitt.edu -----------------------------------
Currently we are looking at stands of DNA that have been stained with Uranyl Acetate and gold labeled, and then deposited on to Collodion film, which the strands stick to, nicely.
However, at high KeV or focused spot the film breaks easily, what I would like to do is use a carbon film, which is more robust but my problem is the DNA will not stick to this.
Perhaps someone who is more expert at DNA imaging could suggest a way to produce a more robust sample?
Many Thanks in advance
David
-- Dr. David C. Bell (612)-624-1677 AEM Manager, IT Chafac dcb-at-umn.edu 16 Shepherd Labs, University of Minnesota http://resolution.umn.edu 100 Union St S.E. Minneapolis, MN USA 55455
Have you tried collodion on carbon? Then you'd have the best of both (put the carbon on the collodion, then use the "back" of the grid). Or try some substrate other than collodion that is more stable in the beam - Butvar, for example.
However, doesn't the DNA "community" usually use carbon films? I've done DNA on carbon - just glow-disharge the carbon film within a few hours before you put the DNA on. It is more annoying than using straight plastic films, but more stable once you get to the 'scope.
Tamara Howard CSHL
On Wed, 30 Aug 2000, David Bell wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Currently we are looking at stands of DNA that have been stained } with Uranyl Acetate and gold labeled, and then deposited on to } Collodion film, which the strands stick to, nicely. } } However, at high KeV or focused spot the film breaks easily, } what I would like to do is use a carbon film, which is more robust } but my problem is the DNA will not stick to this. } } Perhaps someone who is more expert at DNA imaging could suggest } a way to produce a more robust sample? } } Many Thanks in advance } } David } } } -- } Dr. David C. Bell (612)-624-1677 } AEM Manager, IT Chafac dcb-at-umn.edu } 16 Shepherd Labs, University of Minnesota } http://resolution.umn.edu } 100 Union St S.E. Minneapolis, MN USA 55455 } }
I have been asked to identify a figurine said to be "chalkware" has anyone seen or know a definition of that term? Is there a legal definition of chalkware? Would there be a problem calling a plastic filled with 20-30% calcium carbonate - chalkware? The sample was made of gypsum (calcium sulfate) core with a calcium carbonate (whitewash) then painted which makes it what is called carnival-ware. Thanks for any help. Terry Ellis
You could try to evaporate a light coat of carbon over the whole grid after the DNA is stuck and stained. You could also try Formvar which is a little sturdier than collodion.
On Wed, 30 Aug 2000, David Bell wrote:
} Date: Wed, 30 Aug 2000 13:07:30 -0500 } From: David Bell {dcb-at-x32-135.cie.umn.edu} } To: Microscopy-at-sparc5.microscopy.com } Subject: Help Needed: Looking at DNA strands with TEM } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Currently we are looking at stands of DNA that have been stained } with Uranyl Acetate and gold labeled, and then deposited on to } Collodion film, which the strands stick to, nicely. } } However, at high KeV or focused spot the film breaks easily, } what I would like to do is use a carbon film, which is more robust } but my problem is the DNA will not stick to this. } } Perhaps someone who is more expert at DNA imaging could suggest } a way to produce a more robust sample? } } Many Thanks in advance } } David } } } -- } Dr. David C. Bell (612)-624-1677 } AEM Manager, IT Chafac dcb-at-umn.edu } 16 Shepherd Labs, University of Minnesota } http://resolution.umn.edu } 100 Union St S.E. Minneapolis, MN USA 55455 } }
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
I am having serious problems preparing thin foils of a Mg-2.5Ag-2Nd alloy using a Fischione twin jet. I have tried two electrolytes: 5% perchloric in ethanol; 5.3g lithium chloride, 11g magnesium perchlorate, 500ml methanol and 100ml 2-butoxy-ethanol. The main problem I have seems to occur on removal from the Fischione fixture, the specimen continues to react the even the slightest amount of electrolyte-no matter what efforts I take to dilute it. Then we are left with no thin area and lots of corrosion product on the remaining thick foil.
While I haven't used the Fischione polisher, we do manufacture our own electropolisher so I have some experience to share. As our Model 550 is also capable of being used for straight chemical etching (as with HF and Bromine methanol solutions for semiconductor applications) we developed a rinser that will rinse the sample automatically immediately after the perforation is detected. The problem you have is that once a perforation is detected, even the slightest additional etching will quickly etch away the thin area surrounding the hole. If you are doing a purely electrolytic etch, then etching should stop as soon as the power is shut off. Perhaps you are also achieving some chemical etch which will continue until all of the etchant is washed away. A few things you may want to try are:
1) See if there is anyway you can rig up an automatic rinse system to the polisher. This would be ideal, but I do not know how practical it would be.
2) Try to simplify the sample holder so that electrolyte does not get trapped within the holder. If you are experiencing etching after the power is shut off, then any chemicals trapped within the holder will leak out and cause the sample to etch further. Our single jet system places the specimen on a pedestal where the electrolyte cannot become trapped. The sample is not submerged in the electrolyte so the electrolyte quickly washes away. This is also what makes our rinsing attachment easy to implement.
3) If etching still continues after removing the fixture, then you may want to try to adjust the distance between the jets and the sample. With our Model 550 you can change the shape of the dimple in the sample by moving the jet nozzle up or down. Placing the nozzle closer to the sample will provide a larger thin are and will give you additional time to stop the chemical etching before all of the thin area is etched away.
4) With our system you can easily change the LED used in the detection circuit to select a wavelength that is compatible with your material. We have been able to automatically shut off the system in some cases just prior to perforation. If you are able to do this with your system, perhaps the additional etching that occurs as you remove the sample holder will produce a sample with the appropriate thin area.
Of course, my first suggestion would be to buy one of my jet polishers! 8-)
I hope this helps to some degree. If I can be of any additional assistance, please feel free to contact me.
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Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy. Message text written by Milo Kral } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I am having serious problems preparing thin foils of a Mg-2.5Ag-2Nd alloy using a Fischione twin jet. I have tried two electrolytes: 5% perchloric in ethanol; 5.3g lithium chloride, 11g magnesium perchlorate, 500ml methanol and 100ml 2-butoxy-ethanol. The main problem I have seems to occur on removal from the Fischione fixture, the specimen continues to react the even the slightest amount of electrolyte-no matter what efforts I take to dilute it. Then we are left with no thin area and lots of corrosion product on the remaining thick foil.
David, You can stabilize your collodion films with DNA on them through thermal or eb evaporation of carbon layer prior to TEM. Alternatively, you can modify carbon films through alkylamine treatment prior to spreading. Marek.
} } } } } Currently we are looking at stands of DNA that have been stained } } with Uranyl Acetate and gold labeled, and then deposited on to } } Collodion film, which the strands stick to, nicely. } } } } However, at high KeV or focused spot the film breaks easily, } } what I would like to do is use a carbon film, which is more robust } } but my problem is the DNA will not stick to this. } } } } Perhaps someone who is more expert at DNA imaging could suggest } } a way to produce a more robust sample? } } } } Many Thanks in advance } } } } David } } } } } } -- } } Dr. David C. Bell (612)-624-1677 } } AEM Manager, IT Chafac dcb-at-umn.edu } } 16 Shepherd Labs, University of Minnesota } } http://resolution.umn.edu } } 100 Union St S.E. Minneapolis, MN USA 55455 } } } } }
Marek Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories University of California at San Diego address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368 phone: 8588223373 fax: 8588223715 pager: 8586161420 e.mail: mmm-at-ucsd.edu e.pager: 1620024619-at-alphapage.airtouch.com www site: http://mil.ucsd.edu/ ftp site: mil1.ucsd.edu
I am not sure if warming will cure the problem, but is certainly worth trying. As I recall a lot of the AN10,000's had heater circuits built into the detector, with a button on the pulse processor. This may have only been the turret detectors. If present it will be less destructive than hot water.
Best wishes,
Colin
Colin Reid, Electron Microscope Unit, Trinity College Dublin, Dublin 2.
Tel: 353-1-6081820 Fax: 353-1-6770438 Email: creid-at-tcd.ie Home Page: http://www.tcd.ie/Electron_Microscope/emu/home.htm
-----Original Message----- } From: Mary Mager [mailto:mager-at-interchange.ubc.ca] Sent: Wednesday, August 30, 2000 4:37 PM To: Mick Thomas Cc: Microscopy-at-sparc5.microscopy.com
Dear Mick, When I had an increase in peak width in my Kevex last year, I warmed up the detector to remove any water ice that had accumulated in the bottom of the dewar or on the crystal. Turn off the bias, short out the bias plug, pour out the liquid nitrogen, then blow hot air into the dewar until everything is warm. Unfortunately, although the resolution improved 10 eV per channel, the dewar now had poor holding time, so I had to have it re-pumped. Now, everything is great. The other problem can be related to poor grounding in the pre-amp, but that caused excessive dead-time. At 04:01 PM 8/29/00 -0700, you wrote: } } Fellow microscopists, } } We are still using a 15 year old Link AN10000 x-ray detector. The widths } of the peaks have suddenly become much wider. The zero beam or strobe used } to be around 80 eV FWHM, and now is around 360 eV, with no beam in the } column. To the best of my knowledge there is no light in the column, and } the dewar has plenty of LN2 in it, so I am assuming the crystal is } cold. Also, if I disconnect the Lemo connector (carrying the signal from } the detector) the FWHM drops to about 20 eV FWHM. If anyone has any } suggestions I would be very appreciative. } } Thanks, } } Mick Thomas Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
probably the method with the most consistent results (and easy to perform; students do it in our EM-course) is the method by Lang and Mitani (1969). in short, put your cytochrome C stabilized DNA on the collodion film (without carbon) fix with uranylacetate/METOH and perform low angle (approx. 5 °) platinum rotation shadowing (2- 3 nanometer) followed by stationary, vertical (90°) carbon shadowing. specimens are virtually undestroyable and last "forever".
good luck! peter
************************************************** please reply to:
I knew acetylcysteine has been used for airway and stomach mucus but we never had much luck with it with intestinal mucus (rat intestines or human goblet cells cultures). Simon says "bowel" so maybe he means stomach or perhaps he has better luck with intestinal mucus also. I am not an expert with acetylcysteine so I will defer to him on that point. But I can easily believe him when he says that it causes increased mucus secretion on fresh tissue. We have found that most, if not all, of the treatments to remove or "thin" mucus are good only for biochemical preps or fixed tissues since it is especially easy to trigger a goblet cell. Even vigorous washing is enough to set them off.
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-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
The temperature increase in your sample is not only related to the keV and e-dose you use, but also related to the thermal conductivity of your sample, it thickness, surface condition, and its surrounding materials. So I would think it is almost impossible in your case to measure experimentally or to estimate theoretically the temperature increase.
The reason for melting point decrease of any kind of nona-particles is obvious because of the tremendous surface to volume ratio. The surface Gibbs free energy should be much higher in nano-systems, and hence such systems are relatively unstable. That's why most nano-particles have to be kept in aqueous suspension and with additives.
To avoid the problem, try the following:
1. Spot size: 2 or 3 (When I drive TEM, I always first try relatively smaller spot size, usually at 2. Actually I hardly have the need to use spot size 1 on a 200kV scope. The materials I checked range from metals, intermetallic compounds, ceramics, semiconductors and polymers, either thin film or bulk or nano particles.) 2. Different keV, either higher or lower keV could be beneficial to decreasing the damage to a sample depending on which specific heating mechanism dominates. 3. Use a smaller condenser aperture. 4. Coat a very thin C film on your grids before observation.
Have a nice day!
Chao-Ying Ni Rodel Inc. 451 Bellevue Road Newark, DE 19713 (302) 366-0500 ext 2812
-----Original Message----- } From: David R Hull [mailto:David.R.Hull-at-grc.nasa.gov] Sent: Tuesday, August 29, 2000 1:22 PM To: 'Microscopy Listserver'
We have been looking at the use of various catalysts for production carbon nanotubes. Recently we had a sample that behaved in a very unusual way. Upon focusing the electron beam on a cluster of nanotubes, the catalyst particles became fluid and ran through the tubes at about 50-100 nm/sec. I was a little surprised by the movement. The catalyst particles would stop moving within 2-5 seconds.
I have found one reference, Buffat and Borel, Phys. Rev A13, 2287(1976), that showed the melting temperature of gold nanoparticles could be reduced by 600C! for nanoparticles less than 5 nm.
In our case we have cobalt nanoparticles less than 10 nm.
My question is how high a temperature might we expect to heat the sample in the electron beam with the following parameters;
Have others seen this phenomenon? David R. Hull NASA Glenn Research Center at Lewis Field Advanced Metallics Branch Mail Stop 49-1 21000 Brookpark Road Cleveland, OH 44135
The short answer is, that if conditions are right you can get significant local heating under the beam. I (and others before me) have used this technique to "melt" metal chloride crystallites and form pure metal nanoparticles inside the microscope. The key issues are how much energy you are depositing in the specimen (thickness and composition of specimen are important here) and how fast the specimen can carry the heat away. An area of the specimen that does not have good thermal contact to the grid can become very hot. I would suggest looking at Reimer "Transmission Electron Microscopy" starting about page 431 with a section called Specimen Heating.
I have tried some of these calculations before so if you run into problems I might be able to help.
Richard
At 01:21 PM 8/29/00 -0400, David R Hull wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Richard Vanfleet Assistant Professor Advanced Materials Processing and Analysis Center (AMPAC) and Department of Physics
Mail: Department of Physics University of Central Florida 4000 Central Florida Blvd. Orlando, FL 32816-2385
David R. Hull reported that catalyst particles became fluid in nanotubes under TEM examinationŠ and asked for temperature rise.
I guess that there is some lowering of the melting temperature for 10nm cobalt particles, although I do not have right now the data to compute it (in particular the interfacial energy Co-nanotube).
But about temperature rise, you have a very nice situation where inelastic interaction in the nanotubes cluster may lead to a relatively strong energy absorption, while the thermal dissipation by conductivity is low. There is only thermal radiation left for energy dissipation at the surface of the cluster and this may lead to temperature rise of several hundreds of C degrees.
We did a nice experiment (unpublished) 20 years ago with clusters a few microns in diameter containing Ag nanoparticles lying on a carbon film/copper grid. When the electron beam was focused on a cluster (about same diameter, current for "normal observation"), the cluster was instantaneously vaporized and we can follow nucleation, growth and sintering of Ag particles from nm to 50nm running on the film in the same or the next grid opening.
Considering the silver vapour pressure with temperature, this proves that the temperature was by far above 1000 K, more probably toward 1500 K. But again this strange reaction is due to
i) the size of the cluster (significant energy/electron absorption) ii) the low thermal dissipation by conductivity in the film compared to energy absorption iii) the low surface to volume ratio (= low energy dissipation by radiation / energy absorption) in these "large" clusters
It should be pointed out that for isolated nanocrystals, the situation is opposite, the surface to volume is much higher, as the radiation dissipation to energy absorption. In addition the thermal conductivity of the film becomes relatively more important. The (phonon) temperature rise is now only of a few C or tens of C even under strong irradiation.
Yours
Philippe Buffat
} Delivered-To: philippe.buffat-at-epfl.ch } X-Sender: mshull-at-popserve.grc.nasa.gov } Date: Tue, 29 Aug 2000 13:21:37 -0400 } To: "'Microscopy Listserver'" {Microscopy-at-sparc5.microscopy.com} } From: David R Hull {David.R.Hull-at-grc.nasa.gov} } Subject: TEM: Melting and Flow of Nanoparticles During TEM Observation } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We have been looking at the use of various catalysts for production } carbon nanotubes. Recently we had a sample that behaved in a very } unusual way. Upon focusing the electron beam on a cluster of } nanotubes, the catalyst particles became fluid and ran through the } tubes at about 50-100 nm/sec. I was a little surprised by the } movement. The catalyst particles would stop moving within 2-5 } seconds. } } I have found one reference, Buffat and Borel, Phys. Rev A13, } 2287(1976), that showed the melting temperature of gold } nanoparticles could be reduced by 600C! for nanoparticles less than } 5 nm. } } In our case we have cobalt nanoparticles less than 10 nm. } } My question is how high a temperature might we expect to heat the } sample in the electron beam with the following parameters; } } 200 kV, LaB6, 20-30 microamp emmission current, spot size 1, 150 } micron condenser aperture. } } Have others seen this phenomenon? } David R. Hull } NASA Glenn Research Center at Lewis Field } Advanced Metallics Branch } Mail Stop 49-1 } 21000 Brookpark Road } Cleveland, OH 44135 } } (216) 433-3281 } fax (216)977- 7132 } david.r.hull-at-grc.nasa.gov
__________________________________________________________________ Philippe Buffat Ecole Polytechnique Federale de Lausanne (EPFL) Centre Interdepartemental de Microscopie Electronique Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time) E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/ ______________________________ Eudora F2.1 ___________________________
I have a sample of albumin microspheres that need to be embedded and sectioned for TEM. They range from 2-30µm in size. Does anyone have experience embedding such a sample? If I embed them straight into a resin then they may pull out of the resin during sectioning. Any ideas? Karen Kelley Senior Electron Microscopist UF Biotechnology Electron Microscopy Core Lab Box 118525 Gainesville Florida 32611 lab:352-392-1184 fax: 352-846-0251 email: klv-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/staff/karenpage.html
We're going to be fixing and staining Xenopus cells to examine their yolk platelets, and I was hoping someone has had good experience with lipid fixation and suitable fluorescent lipophilic probes for this system. Specifically, we don't want any of our yolk platelets to float away during fixation and membrane permeabilization. I'm also curious as to which Di"X" analogs and/or Bodipy's have worked best for vitellogenin and phosvitin containing yolk. Thanks in advance.
Mike
Michael B. Ferrari
Department of Biological Sciences University of Arkansas Fayetteville, Arkansas 72701 Ph: 501-575-6372 Fax: 501-575-5349 email: ferrari-at-uark.edu http://biology.uark.edu/ferrari
A student working in my lab made ultrathin sections of polymer coated albumin microspheres either lyophilized and embedded in acrylic resin, or dehydrated from an aqueous solution and embedded in epoxy resin. I don't remember any problem with pullout, and she got reasonable looking micrographs in both preps.
Marie
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Dr. Marie E. Cantino Dept. of Physiology and Neurobiology, U-2131 University of Connecticut Storrs, CT 06269-2131 Phone: 860-486-3588 Fax: 860-486-1936
A Leitz Metallux II microscope, which had been stored without air conditioning for some time, recently came into my care. The objectives and other internal lenses are actually obscured by corrosion product. Before anyone gives me a hard time for letting the instrument get into this condition, let me make it quite clear that I am not the one who put it in storage, nor did I know about it until recently. I am looking for advice on how to salvage it.
I removed the objectives from the turret and disassembled one of them as far as I could - just removed the ring holding the lens in the housing. The lens is still in place but the dirt / dried corrosion is plainly exposed. I am reluctant to physically touch the glass for fear of scratching it. Flushing it with a solvent - water? - seems an appropriate first step, but I do not know what liquid to try.
Please excuse me if this is the wrong forum in which to post this query. Any suggestions are welcome. If this instrument appeared on the capital equipment manifest of any department, I could get a P.O. to get it repaired - but it does not. I'm the one who has to fix it if it is going to get fixed. Thanks in advance.
Regards, Andrew T. Werner Shaped Charge Research - Metallurgy Laboratory Schlumberger Reservoir Completions Technology Center 14910 Airline Road, Rosharon, TX 77583-1590 Voice (281) 285-5272 Fax (281) 285-5273
I think one important point needs to be made - are you sure the particles are molten? When you do a lot of TEM, you tend to forget the size and relevant time scales. A typical phonon period is about 10-10 seconds, which is similar in magnitude to the typical attempt time for single-atom diffusion. Hence it is sometimes surprising that samples sit still for long enough for one to take an image! In many cases, particularly at a free surface, what one is observing is a time average of thousands to millions of single-atom diffusion events.
Consequently what appears to be liquid-like behavior may very well be reasonably rapid solid-state diffusion. You do not need to invoke high temperatures in order to explain apparently rapid (in the microscope) motion of nanoparticles. There may be some heating (although direct displacement via radiolytic or knockon processes will be more efficient than heat), but one does not need more than a modest rise to explain the observation in most cases.
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University Tel: (847) 491-3996 Fax: (847) 491-7820 mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
Hi all, I'm trying to put together a proposal to hire someone to run our JSM 840, but I'm not really sure of the salary scale for this kind of position. I would appreciate it if anyone could suggest a range or offer advice on the subject preferably to my personal e-mail at sbucks-at-charter.net Any suggestions would be appreciated. Many Thanks,
You should have no problems finding someone for a starting salary of approximately $60,000.
David Chow E-mail: david.chow-at-nrc.ca {mailto:david.chow-at-nrc.ca}
-----Original Message----- From: Buckingham, Steve [mailto:sbuckingham-at-excellatron.com] Sent: Thursday, August 31, 2000 1:47 PM To: 'Microscopy-at-MSA.Microscopy.Com' Subject: Microscopist position
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Hi all, I'm trying to put together a proposal to hire someone to run our JSM 840, but I'm not really sure of the salary scale for this kind of position. I would appreciate it if anyone could suggest a range or offer advice on the subject preferably to my personal e-mail at sbucks-at-charter.net Any suggestions would be appreciated. Many Thanks,
Is anyone writing imaging programs for the Linux OS? Or a port of NIH-Image for Linux?
Phil -- }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{ Philip Oshel Supervisor, AMFSC and BBPIC Dept. of Animal Health and Biomedical Sciences University of Wisconsin 1656 Linden Drive Madison, WI 53706-1581 voice: (608) 263-4162 fax: (608) 262-7420 (dept. fax)
The tissue we were using were duodenal segments mounted in an ussing chamber, which were washed and then treated with various strains of bugs to look at mechanisms of translocation. It did get rid of the mucus, but I agree with Tom its tough to stop it coming back! Simon
-----Original Message----- } From: Tom Phillips [mailto:PhillipsT-at-missouri.edu] Sent: Thursday, August 31, 2000 8:44 AM To: Microscopy-at-sparc5.microscopy.com
I knew acetylcysteine has been used for airway and stomach mucus but we never had much luck with it with intestinal mucus (rat intestines or human goblet cells cultures). Simon says "bowel" so maybe he means stomach or perhaps he has better luck with intestinal mucus also. I am not an expert with acetylcysteine so I will defer to him on that point. But I can easily believe him when he says that it causes increased mucus secretion on fresh tissue. We have found that most, if not all, of the treatments to remove or "thin" mucus are good only for biochemical preps or fixed tissues since it is especially easy to trigger a goblet cell. Even vigorous washing is enough to set them off.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
The Department of Material Science and Engineering at the University of Delaware has an immediate opening for a lab coordinator/instrument scientist to administer and help further develop the expanding electron microscopy lab in the college of engineering. The successful candidate should have an MS/PhD in MSE or related field and extensive EM laboratory experience. The primary responsibilities of the position include general maintenance of transmission and scanning microscopes (JEOL 2010FEG and 2000 FX TEM, JEOL 840 SEM) and their ancillary sample preparation equipment, user training, facility scheduling, and input on future facility development and instrument acquisition. In addition, it is anticipated that the candidate may pursue collaborative research with faculty in the college and university. The salary for the position will be commensurate with successful candidate qualifications. Applicants should send a cover letter, resume/CV, and list of 3 references to Professor Darrin J. Pochan, 301 Spencer Lab, Materials Science and Engineering, University of Delaware, Newark, DE 19716. Applications will be accepted till September 30, 2000 or until the position is filled.
I often use water first as a cleaner because often it is stuff soluble in aqueous media that is dripped on the lens and often this stuff is not soluble in organic solvents. Then I do an ethanol wash to dry and remove organic soluble stuff.
If I can add to the original question, I have had a great deal of trouble cleaning lenses and especially CCD imagers due to residual dust. I find that cotton swabs (at least those I have) leave an enormous amount of residue behind that cannot be blown off easily. Does anyone have a source of a more dust free equivalent to the cotton swab? Dave --
************************************************************ "Home of the 2000 NCAA Women's Basketball Champions"
Dr. David Knecht Department of Molecular and Cell Biology University of Connecticut 75 N. Eagleville Rd. U-125 Storrs, CT 06269-3125 Knecht-at-uconnvm.uconn.edu 860-486-2200 860-486-4331 (fax) home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html ************************************************************
I don't know about NIH-Image, but there are a large number of imaging programs for Linux. These include:
AVS (see www.avs.com)
Gimp (A Photoshop workalike)
VTK (The Visualization Toolkit, see www.kitware.com)
Image/J is Jave-based and runs on Linux (see http://rsb.info.nih.gov/ij/)
ImageMagick
ImgStar
There is a Mac Emulator called Executor that will allow PC users with Linux to run NIH Image. See www.cs.ubc.ca/spider/ladic/softunix.html
The site above lists a number of IP tools for UNIX, many of which will run on Linux boxes.
billo
On Thu, 31 Aug 2000, Philip Oshel wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Is anyone writing imaging programs for the Linux OS? Or a port of } NIH-Image for Linux? } } Phil } -- } }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{ } Philip Oshel } Supervisor, AMFSC and BBPIC } Dept. of Animal Health and Biomedical Sciences } University of Wisconsin } 1656 Linden Drive } Madison, WI 53706-1581 } voice: (608) 263-4162 } fax: (608) 262-7420 (dept. fax) }
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