Can anybody suggest a supplier from whom I can buy some 0.001" diameter Pt wire? Please include e-mail address if possible.
thanks
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
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Sorry all but it looks like I screwed up again. Over the weekend I was editing the filtering software trying to increase the SPAM/Junk Mail rejection capabilities..... Well, I manaaged to stop all mail to the List which means I was 100% effective in filtering the Junk Mail!! ;-)
In any event, I think things are back on track, and working again. If you tried to post anything between Oct 1 and today you should consider it as being permanently lost in the Ether..
Just resubmit them...
BTW, thanks to all those people who started sending me Email saying " I haven't gotten any mail in the last few days" it started me looking for problems. I guess it's good to know you all miss your Daily Microscopy Email-Fix. Almost sounds addictive. (Hmmm... too bad I can't bottle it an make a few $$)
I have an application for separating various size particles using Thin Layer Chromatography. Question, has anyone prepped the polyester backing, with silica gel coating for SEM?
I have a couple hypothetical protocols I could try but I thought I would send this question out to the masses, to see if anyone has had experience examining TLC plates in the SEM (regluar SEM not an ESEM or VPSEM).
Thanks in Advance, Geoff Williams
Electron Microscope Facility Supervisor Biology Department Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
A colleague on campus is having a problem with their ISI Alpha 9 SEM.
They have diagnosed a failure of the high voltage power supply. Specifically, the gun side of the multi-tap power supply has been fried.
So, they are looking for the power supply for the Alpha 9 or an ISI Mini SEM. If anyone has such a component (or even the whole SEM) for sale or donation, please contact me.
Thank you.
John B.
#################################################################### John J. Bozzola, Ph.D., Director IMAGE (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
} The Chemical Technology Division of Argonne National Laboratory has an } opening for a staff microscopist to perform transmission electron } microscopy in support of the nuclear fuel cycle, waste management, and } other programs as required. This person will also develop new processes } and/or equipment and other technology to support the programs, and } interface with sponsors to maintain existing support and develop new } initiatives. } } This position requires a Ph.D. in chemistry, materials science, or } related discipline and 2-5 years of experience (or equivalent). } } For further information or to send resume, contact David Chamberlain by } electronic mail : chamberlain-at-cmt.anl.gov. } }
Any comments on which would make a better detector, Ge or Si ? only PGT sells Ge detectors and I'm aware of the decrease in resolution with Ge detector. Could someone lists or details the benefits of having a Ge detector as oppose to a Si one.
Sincerely
N.V.Vo
N.V.Vo, Ph.D. Intermagnetics General Corporation 450 Duane Avenue Schenectady, New York 12304 Phone: 518 346 1414 x 3107 Fax: 518 346 6080 E-mail: nvo-at-igc.com www.igc.com
Simon I am not a diatom expert, but I know someone who is. Put your question to Professor David Mann at the Royal Botanic Garden Edinburgh. He eats, sleeps and breathes diatoms (maybe I exaggerate a little!). His email address is d.mann-at-rbge.org.uk
good hunting Chris Jeffree
} From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com Date sent: Wed, 4 Oct 2000 10:04:39 -0500 To: Microscopy-at-sparc5.microscopy.com
Faculty Position Department of Materials Science and Engineering LEHIGH UNIVERSITY
Lehigh seeks to fill a tenure-track position, at the level of associate or assistant professor, in Materials Science and Engineering. Research interests in any area of materials will be considered but the person selected will have a major commitment to the use of electron microscopy. The Materials Department at Lehigh has research strengths in areas of ceramics, metals, polymers and electronic materials. Details can be found from the faculty pages at the departmental web site: http://www.lehigh.edu/~inmatsci/inmatsci.html. It will be considered an advantage if the research interests of the applicant are related to existing research interests in such a way that collaborative work is promoted. Lehigh runs an outstanding electron microscopy facility. Favorable consideration will be given to candidates whose research will involve substantial use of electron microscopy, especially when the microscopy is innovative rather than routine. Lehigh is committed to recruiting, retaining and tenuring women and members of minority groups. Please submit an application by December 15, 2000, to Sharon Coe, Department of Materials Science and Engineering, Lehigh University, 5 East Packer Avenue, Bethlehem PA 18015-3195, USA (slc6-at-lehigh.edu). To discuss the post contact Alwyn Eades (jae5-at-lehigh.edu) at the same address.
================================================================== Dr. Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov ================================================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ==================================================================
The box said "This program requires Win 95/98/NT or better..." so I bought a G3 Mac
The Minnesota Microscopy Society will be holding it's annual Fall Kick-Off event on Thursday, October 12, 2000
This will follow the traditional format of a dinner followed by a talk, which this year is:
"Nature's Jewels and Scientific Tools: Confessions of a Career Diatomist"
by Mark B. Edlund from the Science museum of Minnesota.
Abstract:
Diatoms are single-celled or colonial micro-organisms that possess a cell wall of ornamented opaline silica (biologically-produced glass) and are commonly referred to as the golden-brown algae. It is the wall ornamentation and shape that are used to identify the nearly 25,000 species of living diatoms. Diatoms were "discovered" within the first hundred years of microscopy; Leeuwenhoek himself may be credited with one of the earliest illustrations (1703). Since that time, diatoms have been the jewels, the postage stamps, and the tools of amateur and professional diatomists. Microscopical societies burgeoned in the latter half of the 19th century with members actively exchanging diatomaceous material, slides, and methods and discussing publications and exsicattae. Diatoms figured strongly in the development of light microscopy as test organisms for resolving power; even today, many microscopists have an Amphipleura pellucida slide in the drawer. Transmission electron microscopy revealed much-needed knowledge on the cytology and mechanism of cell wall formation in diatoms, but it has been the scanning electron microscope that has truly changed the field. Ultrastructural details seen in the SEM coupled with "rediscovery" of beautiful cytological work done in the late 1800's and early 1900's radically changed our modern understanding of diatom systematics. Lastly, diatoms have proved to be excellent indicators of water quality and environmental change. Whereas descriptive and biodiversity studies are still common, applied methods now dominate the current scientific efforts.
Speaker: Mark B. Edlund, Research Scientist Science Museum of Minnesota
Mark Edlund has a PhD in Natural Resources from the University of Michigan, and has been specializing in diatoms for the last ten years. Besides field work in the United States, he has done work at Lake Baikal in Siberia, and recently in Mongolia.
Program: 5:30-6:00 PM Social hour 6:00-7:00 PM Dinner 7:00-7:15 PM Business meeting 7:15-8:15 PM Speaker
During the social hour, wine, cheese and crackers will be served. The cost of the meal is $20 per person.
Location: Cherrywood Room, 2nd Floor, St. Paul Students Cente,r St Paul Campus, University of Minnesota, 2017 Buford Ave., St. Paul
Make Your Reservations Today
An accurate head count for the dinner is an absolute necessity. Contact Stuart McKernan by Friday, October 6, to make your reservation (612-624-6009, or stuartm-at-tc.umn.edu).
__________________ Stuart McKernan stuartm-at-tc.umn.edu Office:(612) 626-7594 IT Characterization Facility, University of Minnesota Desk:(612) 624-6009 100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368
You might try a swimming pool supply store. Look for Diatomaceous Earth filter media. As to preparation... Techniques will depend on the type of microscopy employed. If using a high vacuum electron microscope, an electrically conductive film will need to be applied to the diatoms which have been (typically) sprinkled onto double faced tape. The tape will not have to be conductive (if it is - so much the better) if the whole mount is conductively coated.
Woody White McDermott Technology, Inc. http://www.mtiresearch.com/
Is there a problem with the server? I haven't received any messages in the last couple of days. I checked with our server and everything seems to be O.K. Did my address get taken off by accident? In case it was my address is: prutledge-at-ars.usda.gov Thanks,
I would try the two following companies as possible sources of fine platinum wire:
GoodFellow, P.O. Box 628, Doylkestown, PA 18901-9975 FAX: 1-800-283-2020
Refining Systems Inc., P. O. Box 72446, Las Vegas, NV 89170 FAX: 702-368-0933
Both sell high purity and specialty metals in a variety of sizes and shapes. Good luck, WCB
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
At 10:04 AM -0500 10/4/00, "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Have you tried Carolina Biological? They have all kinds of pre-mounted slides for sale.
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Anyone know of a easy, simple way to produce nice powder diffraction samples for illustrating the powder diffraction method for EM students? We use the International Centre for Diffraction Data For student identification of unknown samples. I have tried several methods that usually result in non-electron transparent samples (our TEM KV max is only 120). Any ideas?
_______________________________
Bill Carmichael Electron Microscopy Faculty
Madison Area Technical College 3550 Anderson St. Madison, WI 53704 608-243-4309
Macalester College (St. Paul, Minnesota) has a job opening which involves looking for someone with a microscopy background (as well as electronics skills). The job description is posted at: http://www.macalester.edu/~hr/jobs/job551.html
The description is quite brief, but Macalester has both a Zeiss EM109 TEM and a Zeiss DSM960A SEM. Maintenance and instruction for students/faculty on these machines will be one aspect of this position.
Yes, indeed! You can buy a sack of the stuff there that will keep you busy for all of eternity!
Also check with your neighbors where you live if they have pools and one of them might spare a handful!
Ed Sharpe archivist for SMECC
{ { Subj: Re: Ask a Microscopist - Diatomes Date: 10/4/00 3:20:58 PM US Mountain Standard Time From: nwwhite-at-mcdermott.com (White, Woody N.) To: woodland-at-madasafish.com ('woodland-at-madasafish.com'), Microscopy-at-sparc5.microscopy.com ('Microscopy-at-sparc5.microscopy.com')
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
I have not tried this source, but have heard:
You might try a swimming pool supply store. Look for Diatomaceous Earth filter media. As to preparation... Techniques will depend on the type of microscopy employed. If using a high vacuum electron microscope, an electrically conductive film will need to be applied to the diatoms which have been (typically) sprinkled onto double faced tape. The tape will not have to be conductive (if it is - so much the better) if the whole mount is conductively coated.
Woody White McDermott Technology, Inc. http://www.mtiresearch.com/
Take an oxide powder, grind it up with mortar and pestle and take a carbon coated grid and swipe it across the fines. You can also use two glass slides to grind the powder up.
You can evaporate a number of materials onto a carbon coated grid or onto a cleaved NaCl sample and then float that off on water onto a grid.
You could smoke a Mo wire in air to provide crystals if you leave it in the smoke long enough, you will probably have enough to cover the grid. Heat the wire across the terminals of an evaporator or heat with a torch to generate the white smoke. You could also burn some Mg and get MgO crystals.
If you don't have sufficient particles in a single diffraction pattern to give you rings, take multiple exposures from different areas on the same piece of film.
Incidentally, the MoO3 samples will provide you with a rotation calibration sample.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
} -----Original Message----- } From: William Carmichael [mailto:wcarmichael-at-madison.tec.wi.us] } Sent: Wednesday, October 04, 2000 5:31 PM } To: microscopy-at-sparc5.microscopy.com } Subject: powder TEM diffraction sample prep } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Anyone know of a easy, simple way to produce nice powder } diffraction samples for illustrating the powder diffraction } method for EM students? We use the International Centre for } Diffraction Data For student identification of unknown } samples. I have tried several methods that usually result in } non-electron transparent samples (our TEM KV max is only } 120). Any ideas? } } } _______________________________ } } Bill Carmichael } Electron Microscopy Faculty } } Madison Area Technical College } 3550 Anderson St. } Madison, WI 53704 } 608-243-4309 } } wcarmichael-at-madison.tec.wi.us } http://electron-microscopy.madison.tec.wi.us } } }
What is the difference between diatoms and radiolaria (Actinopods)? They "look" the same to me.
gary g.
At 12:17 PM 10/4/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} --------------------------------------------------------------------------- } } Email: s_woodland-at-madasafish.com } Name: Simon Woodland } School: Kaskenmoor School } } Question: Where can I get some diatoms to put under our microscope? How } should they be prepared and mounted. } } ---------------------------------------------------------------------------
Simon -
You can go to a big home supply store and ask for "diatomaceous earth"; it's used for a lot of filtering applications. You'll get a big sack, so you can use it up by ordering the Lawrence Hall of Science/GEMS manual "River Cutters" (see the Prtoject MICRO URL below), which uses the same materiel to make a model stream bed. Or you can order a prepared slide from a variety of suppliers, Or you can contact Joe Neilly {joe.p.neilly-at-abbott.com} , keeper of Project MICRO's sandpile, and ask for samples of foraminefera sand or the very exotic "star sand". Preparation? Minimal. "Microscopic Explorations", MSA's GEMS manual, will tell you how to make filecard and tape slides that will work well.
Caroline
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
I'm trying to solve a high resolution noise problem with a XL-30 Phillips FESEM. The room that it is in was recently checked and was ok, accept for some acoustic noise. To fix the noise I put up 3 inch sound proofing foam and quieter ducting for the air condition. The noise showed up in an image by a streak about 5 jaggies. After putting up the foam the noise was reduced, but it is still there. The noise is the same for 4mm working distance or a 10mm working distance.
There's a *lot* of difference. About that between a Redwood and an Elephant. Or more. And as distinctive.
Diatoms are about as good a plant as can be found among the protistans. They look like more-or-less fancy silicate pillboxes with lots of little holes.
Radiolaria are amoeboid beasts with a complicated silicate internal "skeleton". They tend to be nested, holey spheres, usually with a more-or-less complex profusion of spikes, needles, and the like.
Crude descriptions, but brief. The best way to see the difference is to get a copy of Ernst Haeckel's book published by Dover -- "Arts Forms in Nature". Beautiful drawings of many different organisms, including Radiolaria and Diatoms.
Phil
} What is the difference between diatoms and radiolaria (Actinopods)? } They "look" the same to me. } } gary g. } -- *** Be famous! Send a Tech Tip or article to Microscopy Today! *** Philip Oshel Technical Editor, Microscopy Today P.O. Box 620068 Middleton, WI 53562-0068 Voice: days (608) 263-4162, evening (608) 833-2885 Fax (608) 836-1969 (please make sure my name is on any fax)
Address for UPS, FedEx, or other couriers: Dept. of Animal Health and Biomedical Sciences University of Wisconsin 1656 Linden Drive Madison, WI 53706-1581 fax: (608) 262-7420 (dept. fax)
What certified (NIST traceable?) TEM mag. calibration samples are available for 20K to over 100K range? Diffraction grating replica sample will not provide sufficient accuracy- too few grating periods "fit" in the image at higher magnifications.
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax
Try Wards Natural History Establishment in upstate New York. The exact address escapes me.
Sam Purdy TechnicalCenter National Steel Corp Trenton MI
} ---------- } From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com } Sent: 4, October 2000, 11:04 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Ask-A-Microscopist: Diatoms } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } -------------------------------------------------------------------------- } - } } Email: s_woodland-at-madasafish.com } Name: Simon Woodland } School: Kaskenmoor School } } Question: Where can I get some diatoms to put under our microscope? How } should they be prepared and mounted. } } -------------------------------------------------------------------------- } - } } }
We have the problems with our JEM-4000EX: the ion pump SIP-1 is not OK! The possible causes are: 1/- leak in vacuum system (after bake out of the SIP); 2/-some contamination into SIP (this SIP was go OK more 40,000 hour); 3/- ??? Does anyone know how to make testing of this situation?
How do to demount the pump, what order of actions?
I am not currently part of the list, so please mail to me directly.
-- Best regards, Anton Gutakovskii mailto:gut-at-thermo.isp.nsc.ru
Yes, mounting the Coolpix 990 to any photomicroscope is absolutely simple via the usual C-mount connection. For the coolpix you have to buy at NIKON the microscope adapter ( here in germany this adapter is around 1000.- DM or approx. 450.- US $; adapter is not listed in Nikon catalogue; contact the microscope section of Nikon) fitting on one side to the thread at the front of the lens and on the other side to your microscope-specific C-mount adapter ( c-mount adapter for Zeiss: buy the more expensive one which has the possibility of correction of the focus plane). The fantastic thing is that you can focus not only via the LCD- screen of the camera ( possible, but not so easy in my opinion) but via an external TV-Monitor in live modus, yielding really smashing results with a resolution satisfying publication needs. With my Coolpix sometimes the camera "hangs up" when the Auto- power off mode is activated (de-activating it helps). Does anybody have this problem as well ?? ( Contact me off-line). What is lacking for the Coolpix 990 at the moment is a cable release, but the Nikon people here in germany tell me it will be available at the end of the year (probably very expensive).
All in all, the Coolpix is the first digital camera under 5000 US-$ I have used or tested which is fully satisfying me in my daily lab routine (photomicroscope (even fluorescence), binocular, makro-photographs) especially due to its simple and clear operation, the possibility to adjust everything (speed, aperture, focus, etc.) manually and its excellent results.
Peter Heimann
************************************************** please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")
Dr. Peter Heimann Developmental Biology & Molecular Pathology; W7-107 University of Bielefeld D 33501 Bielefeld / Germany phone: xx49(0)521-106-5628 / 5627 FAX : xx49(0)521-106-5654 WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie WEB-Site: http://www.uni-bielefeld.de/SFB549 ******************************************************
Wednesday 15 November 2000, Moredun Institute, Edinburgh.
The annual symposium is supported by all area groups in Scotland. The programme for this meeting is comprised of the following three invited talks together with seven contributed talks on a wide range of microscopy with general appeal and a poster display. As in recent years, a comprehensive trade exhibition by leading companies will be present. The Trade Exhibition, Poster Display and Catering will all be together in one central area of this excellent venue.
15/11/2000 Programme
Multi-photon microscopy. Professor Alison Gurney, Dept.of Physiology and Pharmacology, University of Strathclyde.
Ultrastructure eggshell quality assessment: a way forward for captive breeding programmes. Avril Edmond, Dept. Veterinary Anatomy, Glasgow.
Non-invasive imaging of plant tissues using the confocal laser scanning microscope (CLSM) Dr. Karl Oparka, Unit of Cell Biology, SCRI, Dundee.
Functional dynamics of plasmodesmata in sink/source transition leaves. Alison Roberts, Unit of Cell Biology, SCRI, Dundee
COSMIC - a new interdisciplinary facility for advanced spectroscopy, micromanipulation and imaging. Professor Wilson Poon, Dept. of Physics and Astronomy, University of Edinburgh.
Imaging intracellular location of psychoactive drugs using fluorescence microscopy. Richard Horobin, IBLS, Glasgow.
Antigen retrieval allows improved visualisation of V-ATPase immuno-reactivity. Susan Lindsay, Caledonian University, Glasgow.
Techniques in Immunocytochemistry. Peter Jackson, Research Histology Unit, Dept. of Histopathology and Molecular Pathology, Leeds General Infirmary. Talk sponsored by DACO Ltd.
See web site for abstracts from previous meetings
http://www.abdn.ac.uk/emunit/smg99.htm
Kevin Mackenzie Electron Microscope unit Dept Zoology University of Aberdeen Tillydrone avenue Aberdeen AB24 2TZ ----------------- Tel 01224 272847 Fax 01224 272396 email k.s.mackenzie-at-abdn.ac.uk
I hope this isn't seen as an overt advert on the list server, I just want to let people know that this new book is available and hope it may be of general interest. Rest assured that I and my fellow co-authors get very little of the sales proceeds!
A.Patrick Gunning IFR Norwich
ATOMIC FORCE MICROSCOPY FOR BIOLOGISTS
by V J Morris, A R Kirby & A P Gunning
Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the 'natural' conditions usually associated with the light microscope. To biologists AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems.
Contents: Apparatus Basic Principles Macromolecules Interfacial Systems Ordered Macromolecules Cells, Tissue and Biominerals Other Probe Microscopes
Readership: Undergraduates, postgraduates and researchers in biophysics. 352pp Pub. date: Dec 1999 Imperial College Press ISBN 1-86094-199-0 US$51 / £32
I think one can use the "self timer" function of the Coolpix 990 to expose an image without touching the camera body at the time of the exposure.
I can think of no engineering reason why the cable release would need to be expensive when it becomes available. I'll bet any electronics tinkerer could make one for $30.
I'm quite happy with my Cool-Pix 990. If only I could remember 10% of its function modes and how to access them.
High-purity Germanium detectors provide *better* resolution than Si(Li) detectors, and also have lower low-keV background. The higher atomic number of Ge means they have greater x-ray stopping power and therefore higher detection efficiency for the K lines of the heavier elements, and they have therefore been thought of as primarily useful at higher beam accelerating voltages for heavy element analysis. However, this may only be one of their advantages. The better resolution and lower background they offer at low keV suggests they would also be good, and perhaps preferable to Si for light element detection and peak discrimination which could mean more options for low-kV excitation in Field emission SEMs.
What are the flaws in this argument? It would be interesting to hear comments on the above from anyone who has direct knowledge and experience of both types of detectors.
BTW besides PGT, both Gresham and Oxford sell Ge detectors. I am not aware of any others Chris
} From: "NVo-at-IGC.com"-at-sparc5.microscopy.com To: Microscopy-at-sparc5.microscopy.com
Dear Listers:
I am trying to find an enlarger and digital image system to print the conventional and HREM negatives. What kind of enlarger and digital image system are better? manufacturer? prices?
} I'm looking to replace my two year old Polaroid Digital Microscope camera } (SCSI interface with color or black and white, 1600x1200 pixels). The } chip has failed and I don't want to buy another Polaroid camera. I do } primarily black and white photography from a Zeiss optical microscope for } materials engineering applications. I need for the camera to save in tiff } format without changing the dimensions of the objects. I don't need low } light applications. } } Any suggestions? } } Thanks, } } Robin Griffin }
We have found a simple method for preparing drug particles for electron diffraction. We suspend particles in a solvent by sonication for 2-3 seconds and then let the suspension settle for 10-20 seconds. The largest, heavy particles will settle first leaving the thin, light ones in suspension. Then take a drop (20 ul) from the top of the solution, place it on a carbon filmed grid and allow it to dry. The result is a distribution of particles laying flat accross the grid. You need a solvent in which the particles are not soluble. We typically use hexane.
Joe Neilly Abbott Laboratories D-45M, AP31 200 Abbott Park Rd. Abbott Park, IL 60064-6202 voice: (847)-938-5024 fax: (847)-938-5027 e-mail: joe.neilly-at-abbott.com
I have two questions on TEM of DNA and protein complex: 1. which method is the best , or most suitable ,to study such samples? Direct spray to the mica sheet and subsequent metal rotary shadowing, or cytochrome c spreading with metal coating examined by TEM, or atomic force microscopy? 2. Is there any computer software which is designed specifically for analyze these type of images?
This is a bit off the topic of microscopy, but most of us on this server do work in facilities that have XRD units. If anyone is selling, or knows of someone who is selling their XRD, please contact me. I am also curious if anyone has had any experience with the new desk tops like Rigaku has. I plan on demoing one in the future. At $50k-$60k, they are very cost efficient compared to standard XRD units, but I am wary of their performance as an analytical instrument. Lou Solebello
Dear members, I am a PhD student and I use a subscriber to find an answer to a problem I have. I am trying to find a method to fix and process whole fish eggs in order to cut sections for immunohistochemistry. Any suggestions?
Ioannis Vatsos Institute of Aquaculture University of Stirling Scotland, UK
i've installed a 35 and have some issues with a saw-tooth distortion in the scan. it stays constant regardless of KV, but the number of saw-tooths (teeth?) in a frame increases with slower scan rates. i suspected vibration so i mounted the roughing pumps on a big foam block.....no change. i also cycled off the chiller unit with no change, so its probably not mechanical vibrations, unless there is another vibration causing component i'm overlooking.
the distortion is also visible in the data field alphanumerics (they shift and shimmy a little) with the beam turned off, so i don't suspect a HV instability problem.
can i logically deduce that there is an electrical field leakage that is messing up the scan? or a ground loop? what about cable placement? or a problem with the 100V variac (its within 3' of the column).
right now the column is a bit dirty and needs cleaning....i've never seen, for example, dirty apertures cause this type of distortion though.
any suggestions would be appreciated!
brian ---------------------------------------------- Brian McIntyre Electron Microscopy Lab- River Campus Univ of Rochester Rochester, NY 14627 716-275-3058/4875
There currently is no NIST-traceable standard for high-magnification TEM on the market. The next best thing is the MAG*I*CAL TEM calibration sample, which is internally calibrated against the lattice spacing of silicon, a fundamental constant of nature. This statement has been used successfully in the past in fulfillment of the requirements for certification and traceability. The MAG*I*CAL sample allows magnification calibrations across the entire magnification range of TEMs, as well as the camera constant calibration and the image/diffraction pattern rotation calibration.
The sample is marketed by South Bay Technology, TEL: 800-728-2233, 1120 Via Callejon, San Clemente, CA 92673 USA, e-mail: henriks-at-southbaytech.com It is also marketed through various microscopy supply houses, (SPI, EMS, Pelco, etc.) if you have a favorite.
Your request again raises the concern that the microscopy community needs a high-magnification TEM calibration sample that is NIST-certified or NIST-traceable. NIST is currently considering investigating the need for a high-magnification TEM calibration reference, but will not proceed unless there is some interest shown from the user community. It would be helpful to the community in general if you could write a note or send an email to Ms. Nancy Trahey, stating the problem that you have encountered, and reiterating the need for such a NIST-certified or NIST-traceable standard. Her co-ordinates are listed below.
Ms. Nancy M. Trahey Standard Reference Materials Program (232) Engineering Mechanics (202), Room 113 NIST 100 Bureau Drive, Stop 2320 Gaithersburg, MD 20899-2320 Tel: (301) 975-2021 email: nancy.trahey-at-nist.gov
Disclaimer: I 'invented' the MAG*I*CAL TEM calibration sample and retain all bragging rights; it is listed in the Guinness Book of Records as "The World's Smallest Ruler".
John P. McCaffrey, Ph.D. National Research Council of Canada M-50, Montreal Rd. Ottawa, Ontario K1A 0R6 CANADA
-----Original Message----- } From: Vitaly Feingold [mailto:vitalylazar-at-worldnet.att.net] Sent: Wednesday, October 04, 2000 11:54 PM To: .
Dear listers,
What certified (NIST traceable?) TEM mag. calibration samples are available for 20K to over 100K range? Diffraction grating replica sample will not provide sufficient accuracy- too few grating periods "fit" in the image at higher magnifications.
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax
} Any comments on which would make a better detector, Ge or } Si ? only PGT sells Ge detectors and I'm aware of } the decrease in resolution with Ge detector. } Could someone lists or details the benefits of having a Ge } detector as oppose to a Si one.
We are using a "Gem" detector from Oxford Instruments with absoloutely no problems ... 115eV resolution, fast count rates, and very little energy shift with varying time constants. Another benefit is there are no escape peaks for x-ray lines 0~10keV. One drawback may be if you intend to allow the detector to come up to ambient temperatures at times. Si detectors seem to accommodate this, whereas it was suggested at the time of purchase we absolutely not allow this .. but I believe that was simply because the "Gem" was for the most part untested in this respect. I have no idea of this detector's ambient T survivability, we have always kept it at LN2 T.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with Electrodag 502 for secondary electron imaging. The adhesive is a graphite paint in MEK base (a substitute for TV Tube Koat). After ethanol dehydration and CP drying in liquid CO2, the samples are relatively flat or slightly curved. However, once attached to the stub the skins develop a noticeable curl which leaves a gap between the stub surface and sample. In some cases I can see the "trail" in the adhesive left by the skin as it was curling! Filling the gap with more Electrodag is a waste of time because it evaporates and shrinks back away from the sample (thankfully it doesn't seem to produce more curling).
Could anyone suggest a better conductive adhesive? Also, is there any difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based media as to its effect on biological samples?
As an SEM novice any advice you could share with me is greatly appreciated. Thank you very much,
Carla Aiwohi Western Fisheries Research Center Seattle, WA ph: (206) 526-6282 ext.242 fax: (206) 526-6654
Anyone know of a easy, simple way to produce nice powder diffraction samples for illustrating the powder diffraction method for EM students? We use the International Centre for Diffraction Data For student identification of unknown samples. I have tried several methods that usually result in non-electron transparent samples (our TEM KV max is only 120). Any ideas?
Dear Bill, The simplest way I know is to evaporate a layer of Au or Al onto a formvar-covered grid. By spreading the beam, you can get continuous rings, and by using a small selected area you can get discrete spots at various positions around the rings, so you can demonstrate directly that the rings consist of the summed intensities from many crystalites of differing orientations. Good luck. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
Ben, Our Zeiss DSM982 FESEM is also sensitive to acoustic noise. "The quieter the room the more noises we hear." There is no simple answer, just keep trying different things. I was told one lab installed a switch to turn the SEM PC cooling fans off for short periods of time during high resolution work. That is a risky fix if you are over 50 years old.
We strategically padded all connections to the column and redirected the room air flow but have not installed acoustic foam on the walls. Evenings are quieter than days. At 300kx we can detect a backhoe 600 yards away.
Good Luck Jim
} Date: Wed, 4 Oct 2000 19:52:12 -0700 (PDT) } From: Ben Craft {bcraft-at-uci.edu} } X-Sender: bcraft-at-taurus.oac.uci.edu } To: Microscopy-at-sparc5.microscopy.com } Subject: High Resolution noise } MIME-Version: 1.0 } } } } I'm trying to solve a high resolution noise problem with a XL-30 } Phillips FESEM. The room that it is in was recently checked and was ok, } accept for some acoustic noise. To fix the noise I put up 3 inch sound } proofing foam and quieter ducting for the air condition. The noise } showed up in an image by a streak about 5 jaggies. After } putting up the foam the noise was reduced, but it is still there. The } noise is the same for 4mm working distance or a 10mm working distance. } } Any one had a similar problem or have any advice? } } Thanks, } Ben } } } } } ####### } #####\_O -Ben Craft- } ####/\/} } #### /" } ### \ }
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility U-2131 Storrs, CT 06269-2131 bsgphy3-at-uconnvm.uconn.edu 860 486-2914 voice 860 486-1936 fax
You could prepare your own. I find that the diatoms in diatomaceous earth are usually broken and like to prepare my own. You will need a plankton net, some potassium dichromate, and 30% peroxide. Once the diatoms are collected (plankton net tows from shoreline) you can clean them using the 30% peroxide and potassium dichromate. Place the diatoms in a large beaker (and I mean large here) then pour in 15 to 20 mls of peroxide followed by a large pinch of the potasium dichromate. The resultant reaction oxidizes organics leaving the glass frustules of the diatoms unharmed. It is better to be cautious with the amounts that you use as this is an exothermic reaction, sometimes violently so. Not that you'll have an explosion, but I've had to clean up boil overs on a number of occasions. Once the reaction subsides and the solution turns a golden yellow color it is just a matter of washing the diatoms. There are a couple of ways to do this. You could centrifuge the diatoms, then throw out the liquid, add water, centrifuge, throw out the liquid, etc. or you could fill the large beaker with water and let the diatoms settle to the bottom (1-2hrs), carefully pour off the fluid, and add more water, let the diatoms settle, etc. The diatoms will appear as a milky white residue in the bottom of your container (you may have to concentrate the material to really see this). If you need any more info please feel free to contact me.
} --------------------------------------------------------------------------- } } Email: s_woodland-at-madasafish.com } Name: Simon Woodland } School: Kaskenmoor School } } Question: Where can I get some diatoms to put under our microscope? How } should they be prepared and mounted. } } ---------------------------------------------------------------------------
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
Besides Nikon's own release (EU-1 wired remote control) for the CoolPix990, you can purchase a third-party release cable from the CKCpower.com website.
Jim
} } Hi Peter, } } I think one can use the "self timer" function of the Coolpix 990 to } expose an image without touching the camera body at the time of the } exposure. } } I can think of no engineering reason why the cable release would need to } be expensive when it becomes available. I'll bet any electronics } tinkerer could make one for $30. } } I'm quite happy with my Cool-Pix 990. If only I could remember 10% of } its function modes and how to access them. } } Bart Cannon } Cannon Microprobe } Seattle } }
We have a Ge detector and get considerably better resolution with it than we get with an older SiLi detector. The Ge detector has an ultrathin window, the Si detector has a Be window. They are both used for x-ray detection in the 0-20 KeV range.
Kent Ross
----------------------------------------- Kent Ross Research Associate Texas Center for Superconductivity University of Houston 77204-5932 DKROSS-at-uh.edu 713-743-8284 ------------------------------------------
} mounting the Coolpix 990 to any photomicroscope is absolutely } simple via the usual C-mount connection. For the coolpix you have } to buy at NIKON the microscope adapter ... } The fantastic thing is that you can focus not only via the LCD- } screen of the camera ( possible, but not so easy in my opinion) but } via an external TV-Monitor in live modus, yielding really smashing } results with a resolution satisfying publication needs. } With my Coolpix sometimes the camera "hangs up" when the Auto- } power off mode is activated (de-activating it helps). Does anybody } have this problem as well ?? ...
The AC adapter should allow for the video output to remain on ... leastwise, that is how my CP800 works.
} What is lacking for the Coolpix 990 at the moment is a cable } release, ... } } All in all, the Coolpix is the first digital camera under } 5000 US-$ ...
The 990 is indeed feature rich, and the cable release will be needed for microscopy (... altho you should be able to acquire with a steady camera if you take a picture with the time-delay feature ...).
I have realized just lately, altho the CP950 and 990 are the feature-rich and most desireable, the CP800 will work (altho somewhat awkwardly) ... providing the same NTSC/PAL out and lens threads. If you find a lesser expensive 1x C-mount adapter (as available at Optem International {www.optemintl.com} ), then you have a digital camera capable of 1600by1200 for approximately US$800. You should figure an additional $100 for AC adapter ($50) and several NiMH batteries and charger (~$50).
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
Have you considered or looked into the Sony 390P color video camera?? It gives you a live image with an RGB output, C-mount and 1.3 million pixels (effective resolution) and sells for just under $ 3,000.
If you would like any more information, please let me know.
Sincerely,
Gary
Gary Liechty Manager, Technical Products
Allied High Tech Products, Inc. 2376 E. Pacifica Place Rancho Dominguez, CA 90220
310-635-2466 800-675-1118 US and Canada 310-762-6808 Fax
Products for Metallurgical Sample Preparation
www.alliedhightech.com ----- Original Message ----- } From: {"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com} To: {microscopy-at-sparc5.microscopy.com} Sent: Thursday, October 05, 2000 5:54 AM
Simon,
I suspect that diatomaceous earth will have mostly fragments in it. If you let me know your address, I'll try to find an old diatom SEM preparation to send you and I'll also ask some folks here for a fresh sample of whole diatoms that you can prepare for viewing yourself.
Gary,
Being a microscopist, not a microbiologist/paleontologist/sedimentologist (though I've imaged my share of microplankton for them) I can only give a brief overview of the differences between diatoms and radiolaria, but here it is. Rads are floating, single celled marine protozoa that live inside ornate opaline tests (external shells) that look like little Christmas tree ornaments or something you might play badminton with. Their shells have lots of holes, spikes and spines and can be spherical, amorphously latticed, rather bulbous with long spikes from one end, sub-triangular, etc. The cell body inside sends out numerous fine cytoplasmic strands that radiate outward to form a sticky halo that captures food. Diatoms are single-celled plants that live wherever there's moisture - salt water, fresh water, soil, even on whale skin. They can be planktonic (free-floating) or benthic (attached to a substrate) or live on mud or rocks. Some live in the dark, but most need light to photosynthesize. Their siliceous tests are made of two valves that fit together like a pill box and come in two basic shapes, round and pennate. (The pennate ones can look like peanuts or canoes.) Diatom tests are also quite ornamental, with holes, radial designs, or long, sometimes feathery, projections, and sometimes they link together to form long chains. There's also a great variety in size among species.
Dee
*************************************************************** Dee Breger Mgr. SEM/EDX Facility Lamont-Doherty Earth Observatory 61 Route 9W Palisades, NY 10964 USA T: 914/365-8640 F: 914/365-8155
http://www.ldeo.columbia.edu/micro http://www.discovery.com/area/science/micro/micro1.html http://www.lsc.org/antarctica/front.html Journeys in Microspace (Columbia University Press, 1995)
You might also try contacting Bunton Instruments in the US:
{http://www.buntgrp.com/}
They sell microscope adaptors for the CoolPix. The adaptors are not listed on their web site, yet, but call or e-mail them for information. I know they are taking orders for adaptors now and I was told that they would be shipping "around the end of October."
I have no connection with Bunton Instruments other than as a customer.
Gary P. Radice gradice-at-richmond.edu Associate Professor of Biology 804 289 8107 (voice) University of Richmond 804 289 8233 (FAX) Richmond VA 23173 http://www.science.richmond.edu/~radice
You don't mention at what magnifications you see the sawtooth. However, if you see it in the alphanumerics, I can't see that it can be anything other than a problem in your display unit - a 60Hz interference in the line scan drive (but not the scan generator) or something like that. If that is the case, the magnification wouldn't make any difference.
Good luck,
Tony Garratt-Reed
} hi all- } } i've installed a 35 and have some issues with a saw-tooth distortion in the } scan. it stays constant regardless of KV, but the number of saw-tooths } (teeth?) in a frame increases with slower scan rates. i suspected } vibration so i mounted the roughing pumps on a big foam block.....no } change. i also cycled off the chiller unit with no change, so its probably } not mechanical vibrations, unless there is another vibration causing } component i'm overlooking. } } the distortion is also visible in the data field alphanumerics (they shift } and shimmy a little) with the beam turned off, so i don't suspect a HV } instability problem. } } can i logically deduce that there is an electrical field leakage that is } messing up the scan? or a ground loop? what about cable placement? or a } problem with the 100V variac (its within 3' of the column). } } right now the column is a bit dirty and needs cleaning....i've never seen, } for example, dirty apertures cause this type of distortion though. } } any suggestions would be appreciated! } } brian } ---------------------------------------------- } Brian McIntyre } Electron Microscopy Lab- River Campus } Univ of Rochester } Rochester, NY 14627 } 716-275-3058/4875 }
** Anthony J. Garratt-Reed ** MIT Room # 13-1027 ** 77 Massachusetts Avenue ** Cambridge, MA 02139-4307 ** USA ** ** Phone: (+) 1-617-253-4622 ** Fax: (+) 1-617-258-6478 **
We currently have 4 Ge EDX detectors and 1 Si(Li) detector, all from Oxford Instruments. The Ge detectors do have a lower background than the Si(Li) and the resolution is better. The Ge detectors range from 109 ev to 115 ev whereas the Si(Li) is around 132 ev. We routinely use all of them for low-kV work. The Ge detectors do a better job because of the resolution and the lower background. I do SEM of semiconductors, so 30kV is as high as I can go. I can't comment on the Ge detector's performance at higher than that, but they are my favorite detector in the 0.5 kV to 30kV range. N.B.: I have no financial interest in Oxford Instruments; I'm just a satisfied customer.
Chris Jeffree wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } High-purity Germanium detectors provide *better* resolution than } Si(Li) detectors, and also have lower low-keV background. The } higher atomic number of Ge means they have greater x-ray } stopping power and therefore higher detection efficiency for the K } lines of the heavier elements, and they have therefore been thought } of as primarily useful at higher beam accelerating voltages for } heavy element analysis. However, this may only be one of their } advantages. The better resolution and lower background they offer } at low keV suggests they would also be good, and perhaps } preferable to Si for light element detection and peak discrimination } which could mean more options for low-kV excitation in Field } emission SEMs. } } What are the flaws in this argument? } It would be interesting to hear comments on the above from anyone } who has direct knowledge and experience of both types of } detectors. } } BTW besides PGT, both Gresham and Oxford sell Ge detectors. I } am not aware of any others } Chris } } } From: "NVo-at-IGC.com"-at-sparc5.microscopy.com } To: Microscopy-at-sparc5.microscopy.com } Subject: Ge vs Si detector } Date sent: Wed, 4 Oct 2000 13:15:58 -0400 } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Dear Listners: } } } } Any comments on which would make a better detector, Ge or Si ? only PGT } } sells Ge detectors and I'm aware of the decrease in resolution with Ge } } detector. Could someone lists or details the benefits of having a Ge } } detector as oppose to a Si one. } } } } Sincerely } } } } N.V.Vo } } } } } } } } N.V.Vo, Ph.D. } } Intermagnetics General Corporation } } 450 Duane Avenue } } Schenectady, New York 12304 } } Phone: 518 346 1414 x 3107 } } Fax: 518 346 6080 } } E-mail: nvo-at-igc.com } } www.igc.com } } } } } } ========================================== } Dr. Chris Jeffree } University of Edinburgh } BIOSEM - Biological Sciences Electron Microscope Facility } Institute of Cell and Molecular Biology } Daniel Rutherford Building } King's Buildings, Mayfield Road } EDINBURGH, EH9 3JH, Scotland, UK } Tel. #44 (0) 131 650 5345 } FAX. #44 (0) 131 650 6563 } Mobile 07710 585 401 } email c.jeffree-at-ed.ac.uk } =========================================
Our 35C has several times displayed that saw-tooth interference in the past. Each time it was attributed to some ground loop problem. One place to look is in the ribbon aperture. Make sure that the insulators between the aperture strip and holder are properly set or 'not missing'.
I agree that the distortion is not from HV instability which usually displays some darkness or brightness irregularity.
Good luck! _ / \ Sam O. Mancuso /\ /\ Special Metals Corporation (__n__) New Hartford, NY
Brian McIntyre {mcintyre-at-optics.roch To: Microscopy-at-sparc5.microscopy.com ester.edu} cc: Subject: saw-tooth scan distortion: jeol35 10/05/2000 10:04 AM
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
hi all-
i've installed a 35 and have some issues with a saw-tooth distortion in the scan. it stays constant regardless of KV, but the number of saw-tooths (teeth?) in a frame increases with slower scan rates. i suspected vibration so i mounted the roughing pumps on a big foam block.....no change. i also cycled off the chiller unit with no change, so its probably not mechanical vibrations, unless there is another vibration causing component i'm overlooking.
the distortion is also visible in the data field alphanumerics (they shift and shimmy a little) with the beam turned off, so i don't suspect a HV instability problem.
can i logically deduce that there is an electrical field leakage that is messing up the scan? or a ground loop? what about cable placement? or a problem with the 100V variac (its within 3' of the column).
right now the column is a bit dirty and needs cleaning....i've never seen, for example, dirty apertures cause this type of distortion though.
any suggestions would be appreciated!
brian ---------------------------------------------- Brian McIntyre Electron Microscopy Lab- River Campus Univ of Rochester Rochester, NY 14627 716-275-3058/4875
-----Original Message----- } From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com [mailto:"s_woodland-at-madasafish.com"-at-sparc5.microscopy.com] Sent: Wednesday, October 04, 2000 8:05 AM To: Microscopy-at-sparc5.microscopy.com
} } I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with } Electrodag 502 for secondary electron imaging. The adhesive is a graphite } paint in MEK base (a substitute for TV Tube Koat). After ethanol } dehydration and CP drying in liquid CO2, the samples are relatively flat or } slightly curved. However, once attached to the stub the skins develop a } noticeable curl which leaves a gap between the stub surface and sample. In } some cases I can see the "trail" in the adhesive left by the skin as it was } curling! Filling the gap with more Electrodag is a waste of time because } it evaporates and shrinks back away from the sample (thankfully it doesn't } seem to produce more curling). } } Could anyone suggest a better conductive adhesive? Also, is there any } difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based } media as to its effect on biological samples? }
I have found the conductive double-sided adhesive stuff available from several EM suppliers in both disc and tape form to be pretty good. But maybe you can't press your samples down onto it.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
} I was told one lab installed a switch to turn the } SEM PC cooling fans off for short periods of time during high resolution } work. That is a risky fix if you are over 50 years old.
??????????????????????????????
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
} } } } I was told one lab installed a switch to turn the } } SEM PC cooling fans off for short periods of time during high resolution } } work. That is a risky fix if you are over 50 years old. } } ??????????????????????????????
Clarification: I just turned 50. I if I attempted to turned these fans off manually for "a few minutes", chances are the PC processor would shut down on overheat before I remembered to turn them back on. It's an 'out of memory' problem. Jim
} } rtch } } } Ritchie Sims Phone : 64 9 3737599 ext 7713 } Department of Geology Fax : 64 9 3737435 } The University of Auckland email : r.sims-at-auckland.ac.nz } Private Bag 92019 } Auckland } New Zealand }
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility U-2131 Storrs, CT 06269-2131 bsgphy3-at-uconnvm.uconn.edu 860 486-2914 voice 860 486-1936 fax
EG&G ORTEC used to manufacture Germanium EDS detectors too. I do not know whether they still do it since they had become part of Perkin-Elmer. They have, however, a service group which does service Germanium detectors.
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax
This message is made of 100% recycled electrons. -----Original Message----- } From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk} To: "NVo-at-IGC.com"-at-sparc5.microscopy.com {"NVo-at-IGC.com"-at-sparc5.microscopy.com} Cc: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}
Information in your posting strongly suggests ground loop(s) and possibly EM (electromagnetic) fields in the room too. It is possible for local EM interference to affect signal cables without affecting the SEM column much. Did you try EM shielding with sheets of soft Iron? If that makes no difference (it probably wouldn't, since HT set makes no difference for the distortion in your case), than some work needs to be done with shielding/grounding of the various units/components of the SEM. Let us say here, one who has the experience of eliminating 60Hz noise in audio circuits can do the job. Another possibility- did you check DC power supply voltages in your SEM for 60Hz noise? It must be filtered out well.
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax
This message is made of 100% recycled electrons. -----Original Message----- } From: Brian McIntyre {mcintyre-at-optics.rochester.edu} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
your SEM interference problem sounds almost the same as ours that I not long ago put on the listserver - the sawtooths in the scans. Sorry I haven't got an answer to the problem, but I'd be very interested if you manage to solve it! We are going to do a trial with mu-metal in the near future shielding the column of our SEM (Jeol 5410-LV) so I'll let you know how it goes. We suspect electromagnetic field interference.
Good luck,
Mark
******************************************** Mark West, Unidad de Microscopia Electronica, (Electron Microscopy Unit) Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, 04510 Mexico D.F.
Dear Robin, For your application, you may find the Pixera camera to be a cost-effective solution. At the same resolutionoas your current camera, it costs about $1100. At 07:54 AM 10/5/00 -0500, you wrote:
} } I'm looking to replace my two year old Polaroid Digital Microscope camera } } (SCSI interface with color or black and white, 1600x1200 pixels). The } } chip has failed and I don't want to buy another Polaroid camera. I do } } primarily black and white photography from a Zeiss optical microscope for } } materials engineering applications. I need for the camera to save in tiff } } format without changing the dimensions of the objects. I don't need low } } light applications. } } } } Any suggestions? } } } } Thanks, } } } } Robin Griffin } } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Dear Brian, You may have ripple on another power supply, such as lens or scan coils. I always try to put the variac as far from the column as possible. Check for something loose on the column or stage: I've had that effect when the gun HV supply cable was not tied down. Make sure there is a good vibration break (heavy weight) in the foreline hose. The service engineer at ours turned off the rotary pump for a few seconds, to see if it changed. Another source of vibration was a belt-drive pump in another room, but on the same floor beam. A dirty column won't cause this. It may be building vibration. At 10:04 AM 10/5/00 -0400, you wrote: } } hi all- } } i've installed a 35 and have some issues with a saw-tooth distortion in the } scan. it stays constant regardless of KV, but the number of saw-tooths } (teeth?) in a frame increases with slower scan rates. i suspected } vibration so i mounted the roughing pumps on a big foam block.....no } change. i also cycled off the chiller unit with no change, so its probably } not mechanical vibrations, unless there is another vibration causing } component i'm overlooking. } } the distortion is also visible in the data field alphanumerics (they shift } and shimmy a little) with the beam turned off, so i don't suspect a HV } instability problem. } } can i logically deduce that there is an electrical field leakage that is } messing up the scan? or a ground loop? what about cable placement? or a } problem with the 100V variac (its within 3' of the column). } } right now the column is a bit dirty and needs cleaning....i've never seen, } for example, dirty apertures cause this type of distortion though. } } any suggestions would be appreciated! } } brian Good luck!
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
We are pleased to inform you that Boeckeler Instruments, Inc., a local Tucson high technology company, has acquired the RMC Electron Microscopy product line from Ventana Medical Systems.
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During the next few days we will be in the process of moving offices, production lines and inventory so please accept our apologies if you experience any difficulties or delays during this brief transitional period. We will do our utmost to minimize any inconvenience to you by making this move as “transparent” as possible to the outside world and we appreciate your patience & continued support as we relocate the business. Since 1942 Boeckeler has been and still is the industry leader in manufacturing high quality and accurate micrometer and positioner heads. Boeckeler Instruments also manufactures a complete line of video measuring, cross hair and marking products all of which are very complementary to the RMC ultramicrotomes and EM sample preparation instruments.
We look forward to a long and continuing relationship with you as our highly valued customer and hope to be of service to you in the very near future.
If you have any questions or concerns please contact:
Boeckeler Instruments, Inc.
4650 South Butterfield Drive
Tucson, AZ 85714
Tel: (520) 745-0001 Fax: (520) 745-0004
email: info-at-boeckeler.com
Sincerely
Chris Gleeson President & Chief Executive Officer Ventana Medical Systems, Inc.
what you see could be 60-Hz Noise. It follows the characteristics you are describing (more 'teeth' with lower scan rate). Can you do a back-of-the-envelope calculation of how many 'teeth' you get per second (check exposure settings and line times). If it's around 60, you should try to minimize the influence of 60 Hz Noise (ground loops, check all grounding contacts, etc.)
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Brian McIntyre [mailto:mcintyre-at-optics.rochester.edu] Sent: Thursday, October 05, 2000 8:04 AM To: Microscopy-at-sparc5.microscopy.com
hi all-
i've installed a 35 and have some issues with a saw-tooth distortion in the scan. it stays constant regardless of KV, but the number of saw-tooths (teeth?) in a frame increases with slower scan rates. i suspected vibration so i mounted the roughing pumps on a big foam block.....no change. i also cycled off the chiller unit with no change, so its probably not mechanical vibrations, unless there is another vibration causing component i'm overlooking.
the distortion is also visible in the data field alphanumerics (they shift and shimmy a little) with the beam turned off, so i don't suspect a HV instability problem.
can i logically deduce that there is an electrical field leakage that is messing up the scan? or a ground loop? what about cable placement? or a problem with the 100V variac (its within 3' of the column).
right now the column is a bit dirty and needs cleaning....i've never seen, for example, dirty apertures cause this type of distortion though.
any suggestions would be appreciated!
brian ---------------------------------------------- Brian McIntyre Electron Microscopy Lab- River Campus Univ of Rochester Rochester, NY 14627 716-275-3058/4875
It is with great pleasure that I am announcing our 2001 FE-SEM and Image Analysis Courses
When: May 21-25, 2001
Guest Speaker: Dr. David C. Joy Professor, Tennessee University Marin Lagace, Researcher, Hydro-Quebec Research Center
Where: IREQ (Hydro-Quebec Research Centre), Varennes(Montreal), QC., Canada
Topics Covered: FE-SEM Components: gun, lenses, apertures, vacuum technology... Care & feeding of the FEG The secrets of successful FE-Microscopy Vacuum hygiene, cleaning and storing samples Unwanted Beam Interactions What is needed for High Resolution SEM Image Content Getting the most from your SEM How to assess resolution Low Voltage Work - High Resolution at low energy
LVSEM and charging FESEM and Microanalysis Low Energy EDS Work plus so much more
This 3-day workshop will be devided in Lecture and Practical Sessions
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For more information (and/or for reservation) please contact:
Dr Pierre Hovington tel: 450 652-8125 fax: 450 652-8905 e-mail: hovington.pierre-at-ireq.ca
What about using Superglue? I routinely use it when I need a good adhesion of samples. You really do not need a conductive adhesive with a sample thickness of about 3 mm - it's better just to draw a line with carbon adhesive from the edge of a sample to a stud prior conductive coating.
Vladimir Dusevich
-----Original Message----- } From: "carla_aiwohi-at-usgs.gov"-at-sparc5.microscopy.com To: Microscopy-at-sparc5.microscopy.com Sent: 10/5/00 8:46 AM
Hello all,
I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with Electrodag 502 for secondary electron imaging. The adhesive is a graphite paint in MEK base (a substitute for TV Tube Koat). After ethanol dehydration and CP drying in liquid CO2, the samples are relatively flat or slightly curved. However, once attached to the stub the skins develop a noticeable curl which leaves a p between the stub surface and sample. In some cases I can see the "trail" in the adhesive left by the skin as it was curling! Filling the gap with more Electrodag is a waste of time because it evaporates and shrinks back away from the sample (thankfully it doesn't seem to produce more curling).
Could anyone suggest a better conductive adhesive? Also, is there any difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based media as to its effect on biological samples?
As an SEM novice any advice you could share with me is greatly appreciated. Thank you very much,
Carla Aiwohi Western Fisheries Research Center Seattle, WA ph: (206) 526-6282 ext.242 fax: (206) 526-6654
} i've installed a 35 and have some issues with a saw-tooth distortion in } the scan.
My 35C had an aperture heater which used to go into oscillation and produce the kind of problem you describe. It has long since been disconnected. It doesn't seem likely in your case because you see the problem with the beam off, but I thought it was worth mentioning just in case.
Sincerely yours, Andy Buechele
Andrew C. Buechele, Ph.D. Research Professor Vitreous State Laboratory The Catholic University of America 409 Hannan Hall Washington, D.C. 20064 (202) 319-4995 FAX: (202) 319-4469
I need good quality carbon coated grids for viewing purified plant virus particles with TEM. I have had little success with "store bought" coated grids. So unless someone could recommend a good cc grid source, I will need to buy a carbon coater. Should I purchase a carbon coater using flash evaporation or the more expensive carbon turbo evaporator? In addition, could anyone recommend an economically good microtome for thin sectioning plastic embedded specimens?
Thank You,
Nancy L. Robertson, Research Plant Pathologist USDA/ARS National Arctic Plant Germplasm Resources Unit 533 Fireweed Ave. Palmer, Alaska 99645 phone: 907-746-9465 fax:907-746-2677 e-mail: nancy_robertson-at-dnr.state.ak.us
Another way to pick up noise and voltage is to wires wrapped around each other. It is east to test just watch the screen and poke the wires with a wood stick. If there is any problem in the wiring it will show up on the screen. You only have to move them gently.
A wooden stick and light tapping can find a lot of problems. If you are working in the high voltage section make sure it is a long dry stick. It is great for finding intermittent problems. Just start tapping until the problem appears or goes away and then narrow in on the problem.
When you check the frequency if it comes up 120 Hz florescent lights become the number 1 suspect. You could be picking up electrical noise from them or you have something photo sensitive somewhere. Any silicone junction is sensitive to light. The quick test is to turn off the lights. If they are the problem it should at least get weaker.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
----- Original Message ----- } From: "Mike Bode" {mb-at-Soft-Imaging.com} To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, October 05, 2000 6:53 PM
Thank you all for your replies on this topic. Several people seem to like Ge detectors for low keV work, and the replies have mostly been very positive about their resolution and performance. Some authors strongly advocate the use of Ge detectors for low kV, low probe current work with light element samples (See, for example, Block- van Hoek & Pinxter (1993) Chapter 1 in X-ray microanalysis in biology edited by Sigee et al, Cambridge University Press). Their opinion (and apparently yours) stands in contrast with the advice given by all of the XRMA vendors I am speaking to, whose opinion is that Si(Li) is the way to go (granted some of them don't sell Ge, so they would obviously have to advocate Si). So my question is "what is the Achilles heel of Ge detectors?". I am aware for example that their potentially very favourable characteristics at low kV are spoiled by the practical difficulty of manufacturing them without a large dead layer. Is this a fatal flaw in practice for analysis of elements with z {11? Are they less robust, less tolerant of thermal cycling? If anyone can help me get to the bottom of this I would be most grateful.
Chris
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
I am grinding a TEM specimen in a Multipol polishing machine, but a grinding plate is covered with some stains of grayish color whose origin I cannot identify. I tried to apply ethanol, atheton, and methanol to them, but these proved in vain.
Please suggest any cleaning liquid to wipe the grinding plate clean.
Alex
Aleksandr Tikhonovsky, Ph.D. tikhonov-at-mpi-halle.mpg.de tel 49 345 5582 929 Max-Planck-Institut für Mikrostrukturphysik Weinberg 2 D-06120 Halle Germany
You've had a lot of replies, but your symptoms are clear. In most cases, this interference is caused by an electrical device mounted too close to the SEM CRT - An EDS monitor, vacuum gauge controller, etc. If not, you probably have a bad filter or bypass capacitor in a power supply that the CRT uses. Since the effect is also seen in the alpha-numerics, it is directly affecting the view CRT itself. Keeping the area around the CRT clear of other instruments and checking for ripple in the power supplies to the CRT should solve the problem. The effects of external instruments near the view CRT are generally very local and movement of just a few inches can make a huge difference.
It is not mechanical vibrations and the CRTs are not that suseptible to electro-magnetic interference from distant sources.
If the alpha-numerics were not affected, I would suggest checking the relative difference in the noise when using a low and high working distance. If there is a noticably greater noise when at a larger working distance, then electro-magnetic fields affecting the beam would be suspect. The column of an SEM is actually well shielded from EM effects, as the lenses are encased in large ferro-magnetic structures to provide the focussing of the lens effects to the pole pieces. If lower working distances are used (sample closer to the final pole piece) then the effects of EM are reduced since they are primarily induced through the sample chamber and affect the beam from the final pole piece to the sample surface.
However, as I've said, if the alpha-numeric display is also affected, then more than likely the CRT beam is being affected by something close by or by a power supply to the CRT. The high voltage to the CRT (around 10KV) is not a likely suspect as the record CRT is much more suseptable to HV problems and in the JSM-35, as in most SEMs, is fed from the same supply. You would have seen some obvious focus problems in your micrographs if the high voltage was a problem. As the view CRT is an integral part of the electronics console, ground loop problems are unlikely.
The record CRT and view CRT are located close together in this instrument and as I recall they use the same power supplies. The record CRT is probably recording a problem as well, but it may be greatly reduced. The record cycle uses a greatly reduced scan speed, so these variations in the view CRT are going to show up as reduced edge sharpness in your micrographs or not at all if each record scan line is triggered by a zero crossing on the 60Hz input (I don't recall if the JSM-35 does this).
Allen R. Sampson, Owner Advanced Research Systems, St. Charles, Illinois 60174 voice 630.513.7093 fax 630.513.7092
-----Original Message----- } From: Brian McIntyre [SMTP:mcintyre-at-optics.rochester.edu] Sent: Thursday, October 05, 2000 9:04 AM To: Microscopy-at-sparc5.microscopy.com
hi all-
i've installed a 35 and have some issues with a saw-tooth distortion in the scan. it stays constant regardless of KV, but the number of saw-tooths (teeth?) in a frame increases with slower scan rates. i suspected vibration so i mounted the roughing pumps on a big foam block.....no change. i also cycled off the chiller unit with no change, so its probably not mechanical vibrations, unless there is another vibration causing component i'm overlooking.
the distortion is also visible in the data field alphanumerics (they shift and shimmy a little) with the beam turned off, so i don't suspect a HV instability problem.
can i logically deduce that there is an electrical field leakage that is messing up the scan? or a ground loop? what about cable placement? or a problem with the 100V variac (its within 3' of the column).
right now the column is a bit dirty and needs cleaning....i've never seen, for example, dirty apertures cause this type of distortion though.
any suggestions would be appreciated!
brian ---------------------------------------------- Brian McIntyre Electron Microscopy Lab- River Campus Univ of Rochester Rochester, NY 14627 716-275-3058/4875
Have an EDS system attached? The EDS dewars, being generally supported by a cantilever structure, can be quite effective at coupling acoustic vibrations into an SEM. The large area and thin metal construction of the dewar as well as the large momentum arm of the cantilever provide for an amplification of acoustics into the instrument. You might also try to provide some form of isolated support and damping of the EDS system.
The size, shape and construction of the room can also have a considerable effect. Certain configurations can amplify acoustic and construction material mediated effects. Placement of the SEM within a particular room can make a big difference. Particularly bad is the placement in an alcove or bay of a room. Walls close to the sides of an instrument produce complex acoustic patterns. Each wall surface represents a resonator that can vibrate with unexpected sources. An instrument I had placed in this situation in a clean room of a major alphabet soup named manufacturer had incredible sensitivity to door closings and even footsteps in the adjoining airlock and preparation areas.
Your mention of 5 jaggies begs the question - at what scan rate were these viewed? What is the time span for each full scan? The SEM is an incredibly sensitive seismometer. I've seen the effects of waves crashing on the shores of an island as well as the vibrations from an M1-A1 Abrams tank at 40 miles per hour on a nearby proving ground. It can often be difficult to isolate the acoustic effects from the mechanically induced effects (those coupled through solid materials). Most SEMs provide good isolation from high frequency mechanical coupling, but low frequencies are problematic.
Vibrational problems, unlike electro-magnetic problems, affect the beam along it's entire path to some degree. In the column, most of these effects are canceled by the fact that a movement that causes the beam to be deflected outside of it's normal path will be countered by the increased magnetic effect of the lenses as the beam goes off-axis. The greatest effect though, is in the path from the final pole piece to the sample surface. Your determination of the effect being the same from 4mm to 10mm would probably not be true if you measured the difference from 4mm to 25mm.
Allen R. Sampson, Owner Advanced Research Systems, St. Charles, Illinois 60174 voice 630.513.7093 fax 630.513.7092
-----Original Message----- } From: Ben Craft [SMTP:bcraft-at-uci.edu] Sent: Wednesday, October 04, 2000 9:52 PM To: Microscopy-at-sparc5.microscopy.com
I'm trying to solve a high resolution noise problem with a XL-30 Phillips FESEM. The room that it is in was recently checked and was ok, accept for some acoustic noise. To fix the noise I put up 3 inch sound proofing foam and quieter ducting for the air condition. The noise showed up in an image by a streak about 5 jaggies. After putting up the foam the noise was reduced, but it is still there. The noise is the same for 4mm working distance or a 10mm working distance.
Brian McIntyre wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } hi all- } } i've installed a 35 and have some issues with a saw-tooth distortion in the } scan. it stays constant regardless of KV, but the number of saw-tooths } (teeth?) in a frame increases with slower scan rates. i suspected } vibration so i mounted the roughing pumps on a big foam block.....no } change. i also cycled off the chiller unit with no change, so its probably } not mechanical vibrations, unless there is another vibration causing } component i'm overlooking. } } the distortion is also visible in the data field alphanumerics (they shift } and shimmy a little) with the beam turned off, so i don't suspect a HV } instability problem. } } can i logically deduce that there is an electrical field leakage that is } messing up the scan? or a ground loop? what about cable placement? or a } problem with the 100V variac (its within 3' of the column). } } right now the column is a bit dirty and needs cleaning....i've never seen, } for example, dirty apertures cause this type of distortion though. } } any suggestions would be appreciated! } } brian } ---------------------------------------------- } Brian McIntyre } Electron Microscopy Lab- River Campus } Univ of Rochester } Rochester, NY 14627 } 716-275-3058/4875 Brian, You didn't say whether or not the sawtooth shows up on micrographs. If the problem were 60 Hz, they would not be apparent because the record scan speeds are sync'd to the line frequency. If you DO see them on micrographs, start by looking at your low voltage supplies, the CRT HV supply and the scan generator signals.
The problem has nothing to do with your column, since the characters show the problem. CRT HV instabilities should show up more the further from the center of the CRT you get. You should also be getting both light and dark lines as the vertical, as well as the horizontal, will be affected.
If you don't see the above symptoms, look at both scan signals going to the CRT. You may see noise on the horizontal signal, but if the sawtooth is showing up in the image, also, you probably won't see the noise on the horizontal signal to the column, because they aren't syncronized. If they both had the same noise, the image would look fine even though the alphanumerics shimmied.
Because of the alphanumerics, there is some processing of the CRT scan signals after the scan generator that does not happen to the scan signal sent to the mag control/column. A good place to look.
If the problem only occurs on 1 CRT, look at its drivers.
Ken Converse owner Quality Images third party SEM service Delta, PA
If you have not tried our carbon coated grids, we believe we make pretty good ones. If you have tried ours, please let me know what you didn't like and we can correct it since we make them up fresh for each order, and can make them thinner, thicker or whatever you wish. In a worst case scenario, we make what we consider the best evaporator in the business.
John Arnott
--
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
web site http://www.laddresearch.com
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail sales-at-laddresearch.com
Microscope Supplies Since 1955
Nancy_Robertson wrote: }
} } Hi, } } I need good quality carbon coated grids for viewing purified plant virus } particles with TEM. I have had little success with "store bought" coated } grids. So unless someone could recommend a good cc grid source, I will } need to buy a carbon coater. Should I purchase a carbon coater using } flash evaporation or the more expensive carbon turbo evaporator? } In addition, could anyone recommend an economically good microtome for } thin sectioning plastic embedded specimens? } } Thank You, } } Nancy L. Robertson, Research Plant Pathologist } USDA/ARS } National Arctic Plant Germplasm Resources Unit } 533 Fireweed Ave. } Palmer, Alaska 99645 } phone: 907-746-9465 } fax:907-746-2677 } e-mail: nancy_robertson-at-dnr.state.ak.us
------- Forwarded message follows ------- } From: Charles Keith {chkeith-at-cb.uga.edu} To: farmer-at-emlab.cb.uga.edu Date sent: Thu, 5 Oct 2000 09:47:00 -0400 (Eastern Daylight Time) Priority: NORMAL
Mark -
Could you post a request to your microscopy listserv for the index of refraction of Dulbecco-Vogt PBS? I have done so to sci.techniques.microscopy
---------------------- Charles Keith Associate Professor, Department of Cellular Biology (http://www.uga.edu/~cellbio) University of Georgia chkeith-at-cb.uga.edu
John Shields EM Lab 151 Barrow Hall UGA campus 2-4080 fax: 2-4271 jshields-at-cb.uga.edu
Martin Microscope Company of Easley, SC, USA makes an adapter for the Coolpix 990 to fit the eyepiece tube of any microscope (both 23mm and 30mm diameter tubes) for $300. They can also get you an adapter for C-mount. Their phone number is 864-242-3424 or 864-859-2688
} } } Gary Radice {gradice-at-richmond.edu} 10/05/00 01:47PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
You might also try contacting Bunton Instruments in the US:
{http://www.buntgrp.com/}
They sell microscope adaptors for the CoolPix. The adaptors are not listed on their web site, yet, but call or e-mail them for information. I know they are taking orders for adaptors now and I was told that they would be shipping "around the end of October."
I have no connection with Bunton Instruments other than as a customer.
Gary P. Radice gradice-at-richmond.edu Associate Professor of Biology 804 289 8107 (voice) University of Richmond 804 289 8233 (FAX) Richmond VA 23173 http://www.science.richmond.edu/~radice
I thought the only drawback was cost. How much of a cost differential are you being quoted?
I thought all manufacturers had to pick through crystals to find ones suitable for EDS detectors in regard to dead layer, impurity, etc. If good Ge chips are harder to fine, it would mean more expensive detectors.
We haven't tried thermally cycling our detector. I have heard that voltage applied to Si(Li) detectors while warm will lead to a loss of Li, ruining the detector. I don't know what effect it would have on a Ge detector. However, my manager is reluctant to find out if there would be a problem so we just keep our system cool.
Warren
At 09:10 AM 10/6/2000 +0100, you wrote:
} Thank you all for your replies on this topic. Several people seem to } like Ge detectors for low keV work, and the replies have mostly been } very positive about their resolution and performance. Some authors } strongly advocate the use of Ge detectors for low kV, low probe } current work with light element samples (See, for example, Block- } van Hoek & Pinxter (1993) Chapter 1 in X-ray microanalysis in } biology edited by Sigee et al, Cambridge University Press). Their } opinion (and apparently yours) stands in contrast with the advice } given by all of the XRMA vendors I am speaking to, whose opinion is } that Si(Li) is the way to go (granted some of them don't sell Ge, so } they would obviously have to advocate Si). So my question is "what } is the Achilles heel of Ge detectors?". I am aware for example that } their potentially very favourable characteristics at low kV are spoiled } by the practical difficulty of manufacturing them without a large dead } layer. Is this a fatal flaw in practice for analysis of elements with z } {11? Are they less robust, less tolerant of thermal cycling? If } anyone can help me get to the bottom of this I would be most } grateful. } } Chris
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
We have a new Nikon adaptor from Image Systems that allows us to attach our CoolPix 950 to our Zeiss Axioscope 2 via the C-mount or through the eye tube if we wanted to do it that way. Image Systems also sells something they call a cable release adaptor which is a "fancy piece" that hooks onto the camera over the shutter button so that a cable release can be screwed in there. This way we can take a picture without putting pressure on the adaptor set-up or programming the camera to do it. Image Systems in Columbia Maryland can be reached at 410-995-0748.
Just a recent customer.
Chris -- :):):):):):):):):):):):):):):):):):):):):):):):):):):):):):)
Christine A. Brantner, Ph.D.
Biologist/Lab Manager
National Institutes of Health National Institute of Neurological Diseases and Stroke Lab of Neurobiology Section of Analytical Cell Biology Bldg 36, room 2A21 phone 301-435-2803 36 Convent Dr fax 301-480-1485 Bethesda, MD 20892-4062
Nancy - Try Ladd Research (www.laddresearch.com) for high quality grids. I've been using their C-coated grids for several years now and have found that nearly every grid has been perfect. Their grids are somewhat more expensive than those from other manufacturers, but well worth the price if you need good quality.
I haven't tried purchasing grids from every manufacturer so there may be other companies that also produce fine C-coated grids.
Dave Joswiak Dept. of Astronomy Univ. of Washington Seattle, WA 98195 (206)543-7702 joswiak-at-astro.washington.edu
On Thu, 5 Oct 2000, Nancy_Robertson wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Hi, } } I need good quality carbon coated grids for viewing purified plant virus } particles with TEM. I have had little success with "store bought" coated } grids. So unless someone could recommend a good cc grid source, I will } need to buy a carbon coater. Should I purchase a carbon coater using } flash evaporation or the more expensive carbon turbo evaporator? } In addition, could anyone recommend an economically good microtome for } thin sectioning plastic embedded specimens? } } Thank You, } } Nancy L. Robertson, Research Plant Pathologist } USDA/ARS } National Arctic Plant Germplasm Resources Unit } 533 Fireweed Ave. } Palmer, Alaska 99645 } phone: 907-746-9465 } fax:907-746-2677 } e-mail: nancy_robertson-at-dnr.state.ak.us } }
Dear Carla, The double-sided, conductive tabs that are sold in most EM supply catalogues are excellent and provide a good, strong hold. I use DAG 154 from Acheson Colloids, which is colloidal carbon in iso-propanol and ethanol, as a conductive paint to connect the coated surface to the stub. At 06:46 AM 10/5/00 -0700, you wrote: } } Hello all, } } I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with } Electrodag 502 for secondary electron imaging. The adhesive is a graphite } paint in MEK base (a substitute for TV Tube Koat). After ethanol } dehydration and CP drying in liquid CO2, the samples are relatively flat or } slightly curved. However, once attached to the stub the skins develop a } noticeable curl which leaves a gap between the stub surface and sample. In } some cases I can see the "trail" in the adhesive left by the skin as it was } curling! Filling the gap with more Electrodag is a waste of time because } it evaporates and shrinks back away from the sample (thankfully it doesn't } seem to produce more curling). } } Could anyone suggest a better conductive adhesive? Also, is there any } difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based } media as to its effect on biological samples? } } As an SEM novice any advice you could share with me is greatly appreciated. } Thank you very much, } } Carla Aiwohi } Western Fisheries Research Center } Seattle, WA } ph: (206) 526-6282 ext.242 } fax: (206) 526-6654
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
} } I'm looking to replace my two year old Polaroid Digital } Microscope camera ...
If your camera-microscope interface is via 1x c-mount, then the Nikon CP990 and the 1x c-mount adapter is a very good choice ... or convert your microscope tube to c-mount (1x).
A point should be made ... the CP cameras acquire images to an internal memory card ("Compact Flash") ... than cannot acquire direct to a computer. The transfer is instead accomplished at some other time ... either a direct connection, camera to a serial or USB port, or (better/faster) by removing the memory card and using a "Compact Flash" card reader. This might be a hassle for a workstation type microscope, but if freedom of location is important, then probably works better.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
Another drawback is the need to keep them at 77 K all the time (see below).
I have heard that voltage applied to Si(Li) detectors while warm will lead to a loss of Li, ruining the detector.
Not actual loss of Li. To produce a Si(Li) detector, Li atoms are diffused into a Si crystal, which is then subjected to a bias voltage and heated. Li moves toward the cathode in such a way as to compensate for excess electron acceptors, and this results in a large fraction of the detector volume being depleted of carriers and, thus, having a very low dark current. When warmed to room temperature in the presence of bias voltage, Li is redistributed, and the detector no longer has the necessary large depletion region. It is possible to get the same effect with ultra pure Si or Ge, but such detectors--called "intrinsic"--are very expensive due to the stringent limit for impurities. It is now known that in the absence of bias voltage Si(Li) detectors can be at room temperature for a while without damage (although 15 years ago that was not thought to be the case), but Ge(Li) detectors must be kept at 77 K even when no voltage is applied. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
Chris I also have an Oxford intrinsic Ge detector on my SEM and enjoy all the advantages described by previous correspondents. However, as the max voltage on my SEM is 30kV the heaviest element for which I can get K line info (which is preferable to L-lines for quantification) with decent S/N ratio is around Sn (Z = 50). This of course depends on the amount of tin present in your sample.
If you have a Ge detector fitted to a TEM operating at 200 kV, then, in principle, you should be able to detect K lines for all elements. As a result, installation of an intrinsic Ge detector on a TEM may also be a good thing.
Alan Fox
Alan Fox Center for Materials Science and Engineering Naval Postgraduate School Monterey CA 93943
My company is interested in selling a fairly new SEM and we are having a difficult time finding an appropriate place to list it. Can anyone give some suggestions. Also is there any kind of depreciation formula for estimating an appropriate valuation.
Thanks
Raj
********************************************** Dr. Raj Lartius, CEO NovaScan Technologies Iowa State University Research Park 2501 North Loop Drive Ames, Iowa USA 50010
Email: rlartius-at-novascan.com Voice: 515-795-3164 Fax: 515-795-4414 ********************************************** "Innovative Tools to Explore the Microworld"
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EM-listers: I frequently get asked for commercial sources for devices that plunge freeze TEM grids into a cryogen such as liquid ethane. We have our own in-house developed device so I have never kept up with what is on the market. Available devices also seem to come and go so some of my old sources no longer are current. Could I get vendors that have these devices to respond to me off the list. I would appreciate a reference to a web page if one exists and whatever ordering information is available. I will be posting this information on our lab web page and if there is enough interest on this list I can also post the responses.
Thanks Norm Olson
******************************************* Norm Olson Senior Research Electron Microscopist Department of Biological Sciences Lilly Hall of Life Sciences Purdue University West Lafayette, IN 47907
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What other liquids can be used for floating off thin sections other than water? I have a sample that swells in water. It swells slowly in alcohol. Thanks in advance.
I have been asked about the mechanism of Lugol's Iodine as a fixative and I am not sure how it works. Is it a coagulent or anitcoagulent fixative? Does it cross-bind proteins like formaldehyde or formalin? It is used often for fixing dinoflagellate samples and it seems to stabilize the structure of the organisms fairly well without alcohol. Can anyone explain to me the mechanism?
Charles T. Plybon Florida Marine Research Institute Research Staff Aquatic Health Group Tel: (727) 896-8626 e-mail: charles.plybon-at-fwc.state.fl.us
Does any one out there etch L. R. White sections? With sodium-meta-periodate, or something else? Does it really make a difference? Tim
Timothy G. Schneider Director of Electron Microscopy Department of Pathology Room 229 Jefferson Hall Thomas Jefferson University 1020 Locust St. Philadelphia Pa. 19107 215-503-4798 work 610-613-8170 cellular timothy.schneider-at-mail.tju.edu
Fabian-Baber Communications is a multimedia educational production company. We are currently producing a ten-part video series on the human body to be used in middle-school classrooms. We are looking for motion microscopy of human cells, such as blood cells coursing through arteries, cells during mitosis, etc. If you are interested in contributing to this project, or know of a good resource for these images, please contact , Somayyah Siddiqi, at sam-at-fabian-baber.com or 610 623 7812. Thanks so much!
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
I would appreciate the benefit of your experience and knowledge in answering several questions for me as we prepare to purchase our CPD for use in teaching undergraduate biology courses and student/faculty research.
What is your recommendation re the following:
having / not having a viewing port: [I've heard a few recommendations for having a viewing port in a CPD [all accompanied by warnings re the inherent danger]. The only CPD I've used [years ago] had a port which I liked, especially for teaching purposes. I also been asked "Why would you want one?"
chamber size and orientation: vertical or horizontal:
type[s] of specimen holders:
electrically heated vs. hot water heated:
manufacturers and models that you use or have used recently:
costs of fluids and materials used in / with your CPD for a typical run:
compared to other models you might be familiar with: Specifically, is it too much more expensive to run a Polaron jumbo chamber vs. their regular size chamber? [again, for biological material].
What would you look for in your next CPD?
Thanks for your input and time -- they're greatly appreciated.
Lee Hadden Department of Biology Wingate University Wingate, NC 28174 hadden-at-wingate.edu 704.233.8238.
If you are trying to "antigen retrieve" on LR White, there is a nifty paper - Stirling and Graff, 1995, Journal of Histochem. and Cytochem., 43:115-123. "Antigen Unmasking for Immunoelectron Microscopy: Labeling is Improved by Treating with Sodium Ethoxide or Sodium Metaperiodate, then Heating on Retrieval Medium"
Tamara Howard CSHL
On Fri, 6 Oct 2000, Timothy Schneider wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } List severs: } } Does any one out there etch L. R. White sections? With } sodium-meta-periodate, or something else? Does it really make a difference? } Tim } } Timothy G. Schneider } Director of Electron Microscopy } Department of Pathology } Room 229 Jefferson Hall } Thomas Jefferson University } 1020 Locust St. } Philadelphia Pa. 19107 } 215-503-4798 work } 610-613-8170 cellular } timothy.schneider-at-mail.tju.edu } } }
Nancy Robertson wrote: =============================================================== } I need good quality carbon coated grids for viewing purified plant virus } particles with TEM. I have had little success with "store bought" coated grids. } So unless someone could recommend a good cc grid source, I will need to buy a } carbon coater. Should I purchase a carbon coater using flash evaporation or the } more expensive carbon turbo evaporator? In addition, could anyone recommend an } economically good microtome for thin sectioning plastic embedded specimens ? ================================================================ SPI Supplies has produced, in house, with our own (in-house) experienced staff, carbon coated grids, including holey and lacey types, for many years. I hope your less-than-expected results from purchased grids were not our grids, because we really are set up to make them however one does want to make them, mainly because we have our own TEM facilities at our side to inspect what we have made. In any case, many laboratories worldwide depend on the SPI carbon coated grids and we are not aware of any problems that anyone has encountered with them (lots not passing inspection are rejected and not sent to customers). Some want them thicker, some want them thinner, some want them with large holes, some smaller holes, but we can make them however someone wants them to be!
With regard to your question: "Should I purchase a carbon coater using flash evaporation", I will assume that you are asking about the "carbon coaters" that are operated with a rotary vane mechanical pump, usually in tandem with a sputter coater. We are not aware of anyone that has ever gotten acceptable carbon coatings that could be used as support films by TEM using such an equipment approach. Only a diffusion pumped vacuum system, or better, seems to be capable of producing the kind of carbon coatings that would meet that standard. A number of firms offer vacuum evaporators, and we have found the vacuum evaporators made by VG Microtech and also, Denton Vacuum to be especially good for this kind of applications. Quite possibly, others are equally good, but our experience has been with these two particular manufacturers. Note: We do not have any interest, financial or otherwise, in either Denton Vacuum or VG Microtech.
We had the good fortune of learning about a paper soon to be published comparing carbon grids from different commercial sources. The author is P. J. F. Harris, Dept. of Chemistry, U. Reading, Whiteknights, Reading RG6 6AD UK. The title is: Carbonaceous Contaminants on Carbon Support Films for Transmission Electron Microscopy, Carbon 1 (2000).
If Dr. Harris is correct, then one possible solution, since they are made using a different approach (but were not studied by Dr. Harris), might be the Quantifoil grids (my opinion), see URL http://www.2spi.com/catalog/grids/quantifoil-carbon-films.html
They are produced using a different kind of process and therefore would be expected to not have present some of the ambiguous features found on the carbon films studied in the paper mentioned above.
I don't know if this paper has been published yet or not, but I could send to any one requesting it, the entire preprint of the paper via pdf file. Such requests should be made off-line. Give me a day or two to respond.
Disclaimer: SPI Supplies has manufactured since 1975 custom made carbon support films for TEM applications. Grids made by SPI were one of those studied in the above referenced article. However, it is entirely possible that given some of the new demands being made on carbon coated grids generally, especially for high temperature stability, that one should consider as an alternative, the SPI Silicon Nitride Membrane Grids, as described on URL http://www.2spi.com/catalog/instruments/silicon-nitride.html
Chuck
===========================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
Allen R. Sampson wrote: } } Have an EDS system attached? The EDS dewars, being generally supported by } a cantilever structure, can be quite effective at coupling acoustic } vibrations into an SEM. The large area and thin metal construction of the }
EDS dewars: In regard to EDS dewars and acoustic vibrations, if there is enough ice or dirt in the dewar to nucleate bubbles in the LN2 then you might get sufficient vibration as the LN2 boils off. You can often hear this simply by putting your ear against the dewar, especially while the LN2 is at a relatively low level in the dewar. Perhaps in really vibration sensitive systems such as a FESEM this could be another potential problem? The sound of the boiling can range from a continuos fizz to an occasional intense "bump" similar to the effect that you can get just as water begins to boil in a pot on a stove.
Stage motors: We had a bad problem with stage motors once. If you have a motorised stage then any AC ripple from bad power supply filtering remaining on top of the DC holding current can transmit enough vibration via the motor coils to cause problems at high magnification. Sometimes you can "feel" these kinds of vibrations in the motor bodies. Since this holding current is present even when the motors are not actually moving the stage, you have to actually shut off the power to the stage motors to determine if this is the cause.
Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457 Ansto Materials Division Fax: 61-2-9543-7179 PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au Australia www: http://www.ansto.gov.au/
The October meeting of NESM (New England Society for Microscopy) will be held on October 17th at FEI Company in Peabody, MA.
Pre-registration deadline is Friday, October 13th. Please contact Mary McCann, NESM Treasurer at (617)484-7865 or by e-mail: mccanns-at-tiac.net. Registration is $5.00 for members and $20.00 for non-members (this fee includes a current-year membership in NESM).
The meeting will begin with a tour of FEI Company at 5:30pm. Also at this time, Dr. Ronald Mueller of Bristol Myers Squibb will speak on the utility of SEM within the Pharmaceutical Industry. A buffet dinner will follow at 6:00pm.
The scientific presentations will begin at 7:00pm. The speakers are: Dr. Ben Fabry of Harvard School of Public Health, Boston, MA-"Magnetic Twisting Cytometry with Sub-Micron Optical Detection"
Dr. Dan Friend of Brigham and Women's Hospital, Boston, MA-"Thin Section: Freeze Fracture Correlates-A Visit to the Good Old Days"
Barbara Foster-President, Microscopy, Marketing and Education, Springfield, MA- "Bridging the Microscopy-Spectroscopy Chasm"
Thanks to all who sent suggestions and options for connecting the CP990 to Zeiss Axioskop. There are several options. After examining each one, here is a good approach to using the CP990 on an Axioskop.
The first accessory is the attachment of the camera to the 'scope. This is done using an Axioskop trinoc photo port adapter to C-mount. Then, a CP990 thread ring to C-mount adapter is installed. These two adapters then mate the CP990 to the photo port of the Axioskop. Optmem makes all of the pieces needed to make the mating between 'scope and camera. The mate from photo port to C-mount can be done two ways.
Diagnostic Instruments makes a 38mm ISO mount (PN DZA, $275) which also accepts items for Leitz. This same ISO mount onto the Zeiss will accept a PA-41A adapter for Nikon F mount cameras. Optem makes a coupler DC10NN ($80) which mates the 38mm ISO port to C-mount. Then, their 25-70-14-03 ($320) is the CP990 to C-mount adapter. This completes the adaption for a total cost of $275 + $80 + $320 = $675.
Optem's alternate method is the DC-10AM ($150) which directly mounts in the trinoc photo port and provides a C-mount. Then, this mates with the same 25-70-14-03 CP990/C-mount adapter. Total cost of this approach is $150 + $320 = $470.
Zeiss also makes an Axioskop photo port C-mount adapter. This part number is 45 29 95. They can be found used from between $125-$250. Then, the Optem 25-70-14-13 CP990 adapter is used to complete the mate.
When using the CP990, or any other camera for that matter, vibration is a key issue. In this respect the CP990 has an advantage since it does not have a mechanical shutter as do other CCD cameras. But one still needs to reduce vibration when actually taking a pix. This is done via a remote cable release. There are several aftermarket units which perform this function. However, they are rather awkward and large. The best approach is the Nikon EU-1 wired remote ($195 list price). This is a small hand held puck which plugs into the USB port on the CP990. Pressing the large button down half way is like doing so on the CP990 shutter release button. Pressing all the way down takes the pix. The puck also indicates on an LCD how many frames are left.
When doing 'scope work, I recommend the Nikon EH-31 AC adaptor ($50 list price). This avoids drain on the camera batteries.
For storage, the CP990 uses CF type 1 media. The IBM microdrive will not work in this camera. Since the camera stores images in JPEG format, the file size of each image will vary according to how the image compresses. So far, the largest file I have taken is just over 1MB. I am using 128MB CF media. So I can reasonably expect to get about 100 frames per CF. I have two of these CF media. When one is full, it is pulled and replaced with an empty one. The full one is emptied using a Microtech USB CF reader ($38).
Finally, image focusing has always been a problem for me. The CP990 changes all of that. Using the CP990 real time NTSC video output jack, one can watch the image on a separate TV monitor (b/w or color) and adjust for perfect focus. Then take the pix. If you want the camera's LCD unit to stay on indefinitely while shooting, use the setup menu and disable the power off feature.
That's about it for the CP990. Next is the Fuji Finepix S1 Pro. One camp says it will work on a microscope. Another camp says it won't. The camera only does automatic modes when using a lens. When the lens is removed, the camera becomes dumb. What is will actually do remains to be seen. The folks at ElectroImage say that it does work.
For some time, Lugol's Iodine has been used not only to stain certain cells but also to fix and preserve samples of dinoflagellates and other zooplankton. I am curious as to what the mechanism is for stabilizing and fixing with Lugol's. Coagulent or anitcoagulent, does it cross-bind proteins preserving cellular integrity? Can anyone help?
Charles T. Plybon Florida Marine Research Institute Research Staff Aquatic Health Group Tel: (727) 896-8626 e-mail: charles.plybon-at-fwc.state.fl.us
Scott, Glycerol at different concentration (87% as a starter). I use it for PVP and PVA blocks. Marek.
At 15:35 2000-10-06 -0400, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Marek Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories University of California, San Diego
} For storage, the CP990 uses CF type 1 media. The IBM } microdrive will not work in this camera. Since the } camera stores images in JPEG format, the file size of } each image will vary according to how the image } compresses. So far, the largest file I have taken is } just over 1MB.
First ... thanx for compiling this info. However and regarding the statement above ... are you sure the CP990 does not provide for saving TIFFs? My CP800 will save a single high res TIFF to a 8Mb memory card. Presumably one could save ~8-10 CP990 TIFFs to a 128Mb CF card(?) Get back to us on this one ... I'm afraid "JPEG only" would dissuade those who believe in "non-lossy" TIFFs only.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
Hi All, Sorry to bother you with such a strange request. Does anyone have contact information for SeaLite Sciences (associated with bioluminescent markers)? All I have is a web site, and after several days of trying to get through, I've just about given up. Thanks for your help once again, Kristen
At 07:16 AM 10/9/00, you wrote: } Gary writes ... } } } For storage, the CP990 uses CF type 1 media. The IBM } } microdrive will not work in this camera. Since the } } camera stores images in JPEG format, the file size of } } each image will vary according to how the image } } compresses. So far, the largest file I have taken is } } just over 1MB. } } First ... thanx for compiling this info. However and regarding the } statement above ... are you sure the CP990 does not provide for saving } TIFFs? My CP800 will save a single high res TIFF to a 8Mb memory } card. Presumably one could save ~8-10 CP990 TIFFs to a 128Mb CF } card(?) Get back to us on this one ... I'm afraid "JPEG only" would } dissuade those who believe in "non-lossy" TIFFs only.
Yes, it does have a TIFF mode. But this is only available via the Manual mode. I figured that most folks wanted quick and easy access to image capture. If they want/need TIFF, they will have to attack the manual mode.
Larry: We would be very interested in hearing of what you come up with once you have a solution. Mike O'Keefe
Larry Allard wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Alan: } } Just this morning we were discussing the same problem with our Gatan } CCD cameras on our HD-2000, one of which is retractable and used for } TEM imaging, and the other of which is at the end of the GIF for } energy-filtered imaging. These cameras are connected in series for } the water flow, and have very tiny water lines which can easily clog, } even with recirculated water. There is no provision by the } manufacturer to provide for a safety cut-off of the Peltier cooler in } the event of loss of water flow. We have recently experienced a } burn-out of the TEM camera due to a clog in the line, which resulted } in 2 weeks of downtime for repair (nicely done for free by Gatan), } but still no response to queries about some sort of protection. So } we have decided to find an appropriate water flow sensor, and to } install it in an appropriate place in the water line, and to connect } it to the camera controllers to provide a power cut-off when the } water flow diminishes. I'll post the details of the solution when it } is completed. } } Larry } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Dear Listers } } } } Have any other microscopists had problems with Peltier coolers failing on } } water cooled CCD cameras? } } } } In my facility I have three such cameras all plumbed into the microscope } } water system and connected, by the camera manufacturer, into wall sockets. } } On one of the three cameras I have had the Peltier cooler fail four times } } in two years. After the latest failure the manufacturer asked if the } } camera had been run without water. I told him not intentionally but when } } we have power cuts and the power is restored, the camera comes back on but } } the microscope (and water) does not. The camera can therefore run for some } } time without water cooling. Power failures are not uncommon at this } } university! } } } } Many e-mails to the manufacturer have resulted in no response to my } } questions about reliability of the Peltier coolers. The manufacturer is } } treating the failure as an out of warranty issue but I believe there has } } been something wrong with this camera since it was installed. } } } } Any experiences of listservers with Peltier cooler failures would be } } appreciated. Please e-mail me directly. } } } } Regards } } } } Alan W Nicholls, PhD } } Electron Microscopy Service Director } } Research Resources Center - East (M/C 337) } } Room 100 Science and Engineering South Building } } The University of Illinois at Chicago } } 845 West Taylor St } } Chicago, IL 60607-7058 } } } } Tel: 312 996 1227 } } Fax: 312 996 8091 } } Office: Room 110 } } } } Web site www.rrc.uic.edu } } } } } } Alan W Nicholls, PhD } } Electron Microscopy Service Director } } Research Resources Center - East (M/C 337) } } Room 100 Science and Engineering South Building } } The University of Illinois at Chicago } } 845 West Taylor St } } Chicago, IL 60607-7058 } } } } Tel: 312 996 1227 } } Fax: 312 996 8091 } } Office: Room 110 } } } } Web site www.rrc.uic.edu } } Dr. Lawrence F. Allard } Senior Research Staff Member } High Temperature Materials Laboratory } Oak Ridge National Laboratory } 1 Bethel Valley Road } Bldg. 4515, MS 6064 } PO Box 2008 } Oak Ridge, TN 37831-6064 } } 865-574-4981 } 865-576-5413 Fax } allardlfjr-at-ornl.gov
The 2000 Fall Meeting of the Texas Society for Microscopy will be held on October 26, 27, and 28 at the DoubleTree Hotel in Dallas, TX. The hotel is located in Campbell Centre at 8350 N. Central Expressway, Dallas, TX 75206. The phone number for the hotel is 800-222-TREE. A special workshop will be held on Thursday, Oct. 26, entitled "The SEM Today". It will cover the latest information concerning basic SEM, LVSEM, ESEM and detectors.
For more information and/or to register, please contact Pamela Neill, Program Chair TSM Alcon Research LTD 6201 South Freeway Forth Worth, TX 76134-2099 pamela.neill-at-alconlabs.com
We at Ladd may be able to help you. Please let us know what type of work you want to do with it ((target, size of sample, type of sample prep, etc) and I'll let you know what we have available.
Best Regards,
John Arnott
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
web site http://www.laddresearch.com
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail sales-at-laddresearch.com
Microscope Supplies Since 1955
John andrew wrote: } } ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.} } I need to purchase a sputter coater for under 5K. Can } this group recomend what would be a good purchase. } } Thank you } } __________________________________________________ } Do You Yahoo!? } Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free! } http://photos.yahoo.com/
We appreciate the comments Dr. Joswaik and others concerning the quality of Ladd coated grids. Since there are still a number of researchers who coat their own grids, we are pleased to share some of the things we have learned over the past 45 years.
1. Clean the evaporator at least once a day. 2. Change the diffusion pump oil at least every two weeks. 3. Inspect and clean each individual grid prior to coating. 4. Out gas the garbon prior to evaporation. 5. Use fresh chemicals with each batch of grids to be coated.
Hopefully some of this will be of assistance. A recent posting mentioned that a diffusion pump system should be used for carbon coating. We agree 100% with that. In fact we designed the Ladd vacuum system with coated grids in mind by providing for "quick changing" the oil and speedy clean-up.
John Arnott --
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
web site http://www.laddresearch.com
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail sales-at-laddresearch.com
Dear listers, We received several VERY large pieces of horse tendon, half of which was immersed in ethanol and the rest in formalin. The vet didn't know which would be best , so that's how it arrived, via air from New Mexico. We cut it into 1 x 3 mm pieces and processed it routinely, ie, glut, OsO4, ethanol dehydration and PO. We infiltrated in Spurr's and PO for 24 hrs, straight Spurr's for 2 hours and then embedded. What was left just fell out of the blocks . Needless to say, we have a lot of tissue left (still in EtOH and fomalin) and I was wondering if it's worth trying to salvage and if anyone has any suggestions as to the best way to handle this. I did ask the client to please contact me before sending any more tissue but for now they would like us to try and get something from this. Any ideas would really be appreciated. Thank you,
Mary Gail Engle Electron Microscopy & Imaging Facility University of Kentucky
We are considering purchase of a system for quantitative analysis of bacterial and fungal biovolume in soil samples. Could anyone provide info on equipment and preferred method (FITC labeling?). As always, I appreciate your help, Alice.
Alice Dohnalkova Environmental Microbiology Battelle, PNNL MS P7-50 Richland, WA 99352 tel. (509) 372-0692 fax (509) 376-1321
before i go through the effort of separating the ground wire from the rest of the supply system just a few more tidbits to throw out to the list:
1- the distortion in the scan seems to be about 60Hz (more cycles at slower scan rates). in reduced area raster (about 4 frames/sec??) the count is about 15. in "TV" (whatever rate that is on a 35) i get about 2 or 3 cycles.
2- the amplitude of the distortion increases a bit with decreasing KV (i.e. its worse at 10KV than at 30KV...but not much worse). and it seems to be worse at higher magnifications (at 10KX it just about covers the screen left to right; at 500X it is just a couple of bumps).
3- i tightened all the ground lugs....no change.
4- i tightened all the console connectors...no change.
5- i moved the variac farther away from the console...no change.
6- perhaps unrelated.....the conformation of the "spot" realized when the condenser lens current is decreased looks funny. i'm used to a realtively uniform circular cross section. this one looks like a central uniform area with dendritic "arms" radiating outwards...kinda like a spider web pattern. does this mean anything to anyone?? (the final apertures are pretty clean, and it looks funny with any of the three apertures)
thanks for all your previous comments...i'm sure to be closing in on fixing this with your help.
brian ---------------------------------------------- Brian McIntyre Electron Microscopy Lab- River Campus Univ of Rochester Rochester, NY 14627 716-275-3058/4875
Hi Scott. 'Bonne chance', as we say in Canada. A true water substitute (ie in both knife edge wetability and surface tension) is one of those Holy Grail things in ultramicrotomy. You could experiment with liquids like alcohol mixtures, ethylene glycol, etc, but my suggestion is to save yourself a lot of time and simply dry section. It's done more often than you think; water-soluble minerals, reactive metals like Mg, to name a few. Place a grid on the lower part of the diamond knife, section, then tease the section onto the grid with your hair tool. An important point is to then wet the section down onto the grid with something inert (perhaps an alcohol), then dry under a lamp, so that it doesn't fall off the grid on insertion into the TEM. If there is a danger of a somewhat brittle material breaking apart upon sectioning, use a coated grid and some of the segments should be retained.
Do let the Listserver know if someone out there suggests something that works as well as water. You'll have a lot of friends at the next MSA meeting!
Tom Malis Group Leader - Characterization Materials Technology Laboratory Natural Resources Canada (Govt. of Canada) 568 Booth St., Ottawa, Canada ph. 613-992-2310 FAX 613-992-8735 email: malis-at-nrcan.gc.ca
---------- From: Walck, Scott D. [SMTP:walck-at-ppg.com] Sent: Friday, October 06, 2000 3:35 PM To: 'Microscopy-at-MSA.Microscopy.Com' Subject: alternate fluid for ultramicrotomy
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What other liquids can be used for floating off thin sections other than water? I have a sample that swells in water. It swells slowly in alcohol. Thanks in advance.
Just wanted to say thank you for the many replies to my TEM/SEM problem with bacterial culture protocol. Problem solved, thanks again. Kristine C. Fambrough NMSU
Many years ago, I was trying lipid retention embedding for TEM of mycobacterium. GACH seemed to work but sectioning into water was a nightmare. It attracted water from the trough during the ultramicrotome's return stroke which then caused the block to swell. Freon worked better, but the sample from DuPont was quite volatile and evaporated quickly. The workaround was to section into water, but to coat the sides of the block with the thinnest possible film of silicone grease. The grease provided sufficient hydrophobicity to prevent water from jumping onto the face, and if I worked quickly the sections could be placed onto the grids. Needless to say, I only used a glass knife for this. It was a short project, so column contamination from volatiles in the grease wasn't a problem. Maybe a sample of the grease could be put under a high vacuum beforehand to remove volatile components.
Regards, Glen
"Malis, Tom" wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi Scott. 'Bonne chance', as we say in Canada. A true water substitute (ie } in both knife edge wetability and surface tension) is one of those Holy } Grail things in ultramicrotomy. You could experiment with liquids like } alcohol mixtures, ethylene glycol, etc, but my suggestion is to save } yourself a lot of time and simply dry section. It's done more often than } you think; water-soluble minerals, reactive metals like Mg, to name a few. } Place a grid on the lower part of the diamond knife, section, then tease the } section onto the grid with your hair tool. An important point is to then } wet the section down onto the grid with something inert (perhaps an } alcohol), then dry under a lamp, so that it doesn't fall off the grid on } insertion into the TEM. If there is a danger of a somewhat brittle material } breaking apart upon sectioning, use a coated grid and some of the segments } should be retained. } } Do let the Listserver know if someone out there suggests something that } works as well as water. You'll have a lot of friends at the next MSA } meeting! } } Tom Malis } Group Leader - Characterization } Materials Technology Laboratory } Natural Resources Canada (Govt. of Canada) } 568 Booth St., Ottawa, Canada } ph. 613-992-2310 } FAX 613-992-8735 } email: malis-at-nrcan.gc.ca } } ---------- } From: Walck, Scott D. [SMTP:walck-at-ppg.com] } Sent: Friday, October 06, 2000 3:35 PM } To: 'Microscopy-at-MSA.Microscopy.Com' } Subject: alternate fluid for ultramicrotomy } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } What other liquids can be used for floating off thin sections other } than } water? I have a sample that swells in water. It swells slowly in } alcohol. } Thanks in advance. } } -Scott Walck }
--
Glen MacDonald Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 glenmac-at-u.washington.edu (206) 616-4156 (206) 616-1828 fax ************************************************************************ C:} The box said "Requires Windows95 or better". So I bought a Macintosh. ************************************************************************
Hello Guys I am a novice user of a SEM (LEO 1450 to be precise) equipment which was just installed in our central science lab. Is there a way to clean stubs for reuse or are stubs disposable once used. ( This may sound naive) soji Mr. O. O. ILORI DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING OBAFEMI AWOLOWO UNIVERSITY, ILE-IFE, OSUN STATE NIGERIA. email: sojilori-at-oauife.edu.ng
Hi All, I'm tring to find users of JEOL JSM-840 (or similar) microscopes in the Atlanta area. In addition, I need to find contract SEM analysis labs here that might be able to run some fast turnaround samples for me (mostly cross sections). I know several of you contacted me recently after I posted for a microscopist, but I no longer have the information. Could you please send mail again to sbuckingham-at-excellatron.com . Many Thanks, Steve
Steve Buckingham Excellatron Solid State LLC 1460 Roswell Street, Suite J Smyrna, GA 30080 phone 770 438 2201 fax 617 812 5920
Dear Brian, The profile of the spot you describe sounds like an under-saturated filament. At 08:14 AM 10/10/00 -0400, you wrote:
} hi again- } } before i go through the effort of separating the ground wire from the rest } of the supply system just a few more tidbits to throw out to the list: } } 1- the distortion in the scan seems to be about 60Hz (more cycles at } slower scan rates). in reduced area raster (about 4 frames/sec??) the } count is about 15. in "TV" (whatever rate that is on a 35) i get about 2 } or 3 cycles. } } 2- the amplitude of the distortion increases a bit with decreasing KV } (i.e. its worse at 10KV than at 30KV...but not much worse). and it seems } to be worse at higher magnifications (at 10KX it just about covers the } screen left to right; at 500X it is just a couple of bumps). } } 3- i tightened all the ground lugs....no change. } } 4- i tightened all the console connectors...no change. } } 5- i moved the variac farther away from the console...no change. } } 6- perhaps unrelated.....the conformation of the "spot" realized when the } condenser lens current is decreased looks funny. i'm used to a realtively } uniform circular cross section. this one looks like a central uniform area } with dendritic "arms" radiating outwards...kinda like a spider web pattern. } does this mean anything to anyone?? (the final apertures are pretty } clean, and it looks funny with any of the three apertures) } } thanks for all your previous comments...i'm sure to be closing in on fixing } this with your help. } } brian } ---------------------------------------------- } Brian McIntyre
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
} ... } } 1- the distortion in the scan seems to be about 60Hz
This might imply you have a ground loop problem. Possibly one of the other devices (computer, EDX, ...) is providing a ground connection independent of your primary ground(?)
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
)( / _ Jonathan Lipkin () \ _ Assistant Professor of Multimedia Design )( / _ Ramapo College of New Jersey () \ _ http://arts.ramapo.edu/lipkin )( / _ 201/ 684/ 7056/
Dear listserv:
Dee Breger was kind enough to forward this message for me. I'm writing a book on digital photography, to be published by Harry N. Abrams next year. The book will include a section on applied digital imaging, and I am particularly interested in scientific imaging. I am hoping that some of you folks might be able to provide some information.
For the purposes of the book, I am defining digital imaging as that which describes a scene using discrete digital information in a raster grid, or bitmap. So, I am particularly interested in scientific imaging applications which gather information in this way.
I would be interested to hear if there are other scientific imaging systems (DEE: besides SEMs) which collect information in this manner. For instance, it sounds as though atomic force microscopes work in this way: as they drag a stylus across a surface, the position is recorded (x and y), along with the height (z). This is later used to re-create the image in a raster grid (the fact that the AFM does not use light makes it another interesting foil to 'traditional' digital photography). How then, are colors in the final output determined from the data collected? I would also, of course, love to see any images you might have to show.
Thanks in advance, Jonathan Lipkin (jlipkin-at-ramapo.edu)
DEE: I'll forward to Jonathan any replies that are publically posted to the listserver. He also needs other detailed technical information on digital image formation which can be far more comprehensively clarified and amplified by some of the experts among my fellow listers than by me, though I've already given him the broad picture for analog scopes like my dear old Cambridge 250. His SEM impressions, which I'll pass along verbatim, include (1) "SEMs collect information, used to fill a raster grid, through the use of a PMT", (2) the signal is sent directly to the crt in analog form -I always had thought that the pmt sent out digital info, but it sounds like it just sends out analog data (a waveform? though it couldn't be a waveform if the raster grid is discrete in two dimensions)" and (3) "as the secondary electrons come off the sample, they hit a detector which generates a digital number (redundant) which is then placed in a pixel of a bitmap."
Many thanks to any who can help!
*************************************************************** Dee Breger Mgr. SEM/EDX Facility Lamont-Doherty Earth Observatory 61 Route 9W Palisades, NY 10964 USA T: 914/365-8640 F: 914/365-8155
http://www.ldeo.columbia.edu/micro http://www.discovery.com/area/science/micro/micro1.html http://www.lsc.org/antarctica/front.html Journeys in Microspace (Columbia University Press, 1995)
Does anyone in the Chicago area have an ultrasonic disc cutter we can use temporarily? Alternatively, does anyone have one they are not using and would be willing to sell or donate?
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University Tel: (847) 491-3996 Fax: (847) 491-7820 mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
If you aren't able to find an ultrasonic cutter locally to use, please let me know. Perhaps I can arrange to have one of our SoniCut 380 systems sent from our applications lab for short term use or maybe we could arrange to cut some samples for you here. Please let me know if I can be of help.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by "L. D. Marks" } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Does anyone in the Chicago area have an ultrasonic disc cutter we can use temporarily? Alternatively, does anyone have one they are not using and would be willing to sell or donate?
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University Tel: (847) 491-3996 Fax: (847) 491-7820 mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
Well we've got a new vacuum evaporator and it has been so long since I've had one I've forgotten how to treat the bell jar to reduce the effort it takes to keep it clean. However, I do recall that there were little tricks to cleaning and treating the glass and other surfaces. Anyone care to shed a little light my darkened memory of these procedures?
TIA
Rick A. Harris, Director Microscopy and Imaging Facility Section of Molecular and Cellular Biology 1241 Life Sciences Addition University of California Davis, CA 530 752 2914 530 754 7536 fax http://katie.ucdavis.edu raharris-at-ucdavis.edu
Another fault this weekend, this time the subscription list became corrupted. This mean't a significant number of you (~ 1500 ) "disappeared" for a few days and hence did not receive mail. Iunfortunatley did not notice since the mail did go through to the rest of the subscribers on the list. In any event , I have restored the list to it's Oct 6th backup copy.
To those of you that unsubscribed between Oct 6th and Oct 10th. I apologize and ask you to unsubscribe again. I, unfortunately, do not keep records of those of you that have left.
I am trying to get the internal(cytoplasmic) side of a prepared erythrocyte ghost's plasma membrane attached to coverslip, so that the external side is free in water solution. Does anyone have an experience with this or suggest the reagent that preferably interacts with inner leaflet of plasma membrane or cytoskeletal protein ? How should I test the external side is free ?
Any suggestions on this problem would be greatly welcomed. Thank you
Nguyen
Lab. of Appl. Microbiology Dep. of Biochemistry Faculty of Agriculture Tohoku University Sendai, Japan.
The easiest way to keep your bell jar clean is to wipe a thin film of detergent over the surface of the clean glass and pump down to dry. After each coating run simply rinse off the glass, the detergent comes away and takes the coating with it. Recoat with detergent and dry.
Train ALL users in this technique, insist they clean the glass if they "FORGOT" to do it after their last run, and you will always have a clear, clean bell jar. Regards JVN Rick Harris wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Well we've got a new vacuum evaporator and it has been so long since I've } had one I've forgotten how to treat the bell jar to reduce the effort it } takes to keep it clean. However, I do recall that there were little tricks } to cleaning and treating the glass and other surfaces. Anyone care to shed } a little light my darkened memory of these procedures? } } TIA } } Rick A. Harris, Director } Microscopy and Imaging Facility } Section of Molecular and Cellular Biology } 1241 Life Sciences Addition } University of California } Davis, CA } 530 752 2914 } 530 754 7536 fax } http://katie.ucdavis.edu } raharris-at-ucdavis.edu
-- **************************************************** John V Nailon Operations Manager Centre for Microscopy and Microanalysis The University of Queensland St. Lucia Queensland 4072 Phone: +61-7-3365-4214 Fax: +61-7-3365-4422 WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon ****************************************************
I'm getting ready to purchase a new sputter coater for our FESEM. I'd like to hear how lucky others were with using a "regular" sputter coater for high resolution SEM work. Currently, there is a noise problem with our SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm ZrO2 close-packed particles, and 4nm pores in glass using a thin coating of Au/Pd applied with an old Polaron. One thing I'm worried about is = once the noise is fixed and I can resolve the published 1.5nm resolution will I be able to resolve the grains in the coating?
Rick A. Harris wrote: =============================================================== Well we've got a new vacuum evaporator and it has been so long since I've had one I've forgotten how to treat the bell jar to reduce the effort it takes to keep it clean. However, I do recall that there were little tricks to cleaning and treating the glass and other surfaces. Anyone care to shed a little light my darkened memory of these procedures? =============================================================== One product that will do this is called Bell Bright™. A companion product for metal surfaces is called Metal Bright™. Both are described in detail on the SPI website at URL http://www.2spi.com/catalog/supp/supp3.html
The concept is to leave a thin water soluble polymer behind on the clean glass (or metal) surface, and then when it is time to remove the evaporated deposited, a lint free cotton wiper and water alone will wipe away most of the material (as the originally deposited polymer film dissolves away).
Disclaimer: SPI Supplies "invented" this product in the late 1970's and has offered it ever since. We would have a vested interest in seeing its use increase.
Chuck
PS: Remember that we are trying to become 100% paperless and the only way that we can manage this kind of correspondence, in a paperless environment, is for our correspondents to always reply by way of "reply" on their software so that the entire string of correspondence on that topic gets returned to us, and the entire correspondence history can be kept in one place.
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco online che stiamo organizzando perche' anche cittadini come lei (che non votino affatto radicale o che lo facciano, che non votino o non intendano piu' votare) possano partecipare alle nostre decisioni, essere presenti o rappresentati nei nostri organi dirigenti.
In vista delle elezioni politiche occorre tentare di allearsi con il Polo o con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni non democratiche? Quali Riforme istituzionali, politiche, del lavoro, dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi" come finora?
Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a questo gioco, lo preannunci e registri cliccando qui: http://www.radicali.it/p_register/
Mi scusi ancora. A presto.
Emma Bonino
PS Questa email e' stata inviata nel rispetto della legge sulla privacy; se desidera maggiori informazioni o se intende cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/
Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco online che stiamo organizzando perche' anche cittadini come lei (che non votino affatto radicale o che lo facciano, che non votino o non intendano piu' votare) possano partecipare alle nostre decisioni, essere presenti o rappresentati nei nostri organi dirigenti.
In vista delle elezioni politiche occorre tentare di allearsi con il Polo o con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni non democratiche? Quali Riforme istituzionali, politiche, del lavoro, dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi" come finora?
Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a questo gioco, lo preannunci e registri cliccando qui: http://www.radicali.it/p_register/
Mi scusi ancora. A presto.
Emma Bonino
PS Questa email e' stata inviata nel rispetto della legge sulla privacy; se desidera maggiori informazioni o se intende cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/
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HERE'S HOW THIS AMAZING PROGRAM WILL MAKE YOU THOUSANDS OF DOLLAR$
INSTRUCTIONS:
This method of raising capital REALLY WORKS 100% EVERY TIME. I am sure that you could use up to $50,000 or more in the next 90 days. Before you say "BULL... ", please read this program carefully.
This is not a chain letter, but a perfectly legal money making opportunity. Basically, this is what you do: As with all multi-level businesses, we build our business by recruiting new partners and selling our products. Every state in the USA allows you to recruit new multi-level business partners, and we offer a product for EVERY dollar sent. YOUR ORDERS COME BY MAIL AND ARE FILLED BY E-MAIL, so you are not involved in personal selling. You do it privately in your own home, store or office. This is the GREATEST Multi-Level Mail Order Marketing anywhere.
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2. IMPORTANT DO NOT alter the names of the people who are listed next to each report, or their sequence on the list, in any way other than is instructed below in steps "a" through "f" or you will lose out on the majority of your profits. Once you understand the way this works, you'll also see how it doesn't work if you change it. Remember, this method has been tested,and if you alter it, it will not work. a. Look below for the listing of available reports. b. After you've ordered the four reports, take this advertisement and remove the name and address under REPORT #4. This person has made it through the cycle and is no doubt counting their $50,000! c. Move the name and address under REPORT #3 down to REPORT #4. d. Move the name and address under REPORT #2 down to REPORT #3. e. Move the name and address under REPORT #1 down to REPORT #2. f. Insert your name/address in the REPORT #1 position.
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AVAILABLE REPORTS
*** Order Each REPORT by NUMBER and NAME *** Notes: -- ALWAYS SEND $5 CASH (U.S. CURRENCY) FOR EACH REPORT. CHECKS NOT ACCEPTED. -- ALWAYS SEND YOUR ORDER VIA FIRST CLASS MAIL. -- Make sure the cash is concealed by wrapping it in at least two sheets of paper. On one of those sheets of paper, include: (a) the number & name of the report you are ordering, (b) your e-mail address, and (c) your name & postal address.
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Before you make your decision as to whether or not you participate in this program. Please answer one question. DO YOU WANT TO CHANGE YOUR LIFE? If the answer is yes, please look at the following facts about this program:
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Thank you for your time and consideration.
PLEASE NOTE: If you need help with starting a business, registering a business name, learning how income tax is handled, etc., contact your localoffice of the Small Business Administration (a Federal Agency) 1-800-827-5722 for free help and answers to questions. Also, the InternalRevenue Service offers free help via telephone and free seminars aboutbusiness tax requirements. Your earnings are highly dependant on youractivities and advertising. The information contained on this site and in the report constitutes no guarantees stated nor implied. In the event that it is determined that this site or report constitutes a guarantee of any kind, that guarantee is now void. The earnings amounts listed on this site and in the report are estimates only. If you have any questions of the legality of this program, contact the Office of Associate Director for Marketing Practices, Federal Trade Commission, Bureau of Consumer Protection in Washington, DC.
///////////////////////////////////////////////////////////////// Remove at qmkx-at-netscape.net /////////////////////////////////////////////////////////////////
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id GAA07967 for dist-Microscopy; Wed, 11 Oct 2000 06:27:21 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id GAA07964 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Wed, 11 Oct 2000 06:26:50 -0500 (CDT) Received: from servizi-2.padova.com (elezioniradicali-2.padova.com [212.131.155.43]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id GAA07957 for {Microscopy-at-sparc5.microscopy.com} ; Wed, 11 Oct 2000 06:26:37 -0500 (CDT) Received: from servizi.padova.com (unverified [212.131.155.42]) by servizi-2.padova.com (Rockliffe SMTPRA 4.2.2) with ESMTP id {B0000005729-at-servizi-2.padova.com} for {mlistc3_emma.bonino-at-elezioniradicali-2.padova.com} ; Wed, 11 Oct 2000 11:17:42 +0200 Received: from padova08 (unverified [212.131.155.44]) by servizi.padova.com (Rockliffe SMTPRA 4.2.2) with SMTP id {B0000036121-at-servizi.padova.com} for {mlistc3_emma.bonino-at-elezioniradicali-2.padova.com} ; Wed, 11 Oct 2000 11:22:01 +0200 Message-ID: {001401c03365$51216370$2c9b83d4-at-pol.it} Reply-To: "Emma Bonino" {emma.bonino-at-elezioniradicali.padova.com}
Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco online che stiamo organizzando perche' anche cittadini come lei (che non votino affatto radicale o che lo facciano, che non votino o non intendano piu' votare) possano partecipare alle nostre decisioni, essere presenti o rappresentati nei nostri organi dirigenti.
In vista delle elezioni politiche occorre tentare di allearsi con il Polo o con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni non democratiche? Quali Riforme istituzionali, politiche, del lavoro, dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi" come finora?
Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a questo gioco, lo preannunci e registri cliccando qui: http://www.radicali.it/p_register/
Mi scusi ancora. A presto.
Emma Bonino
PS Questa email e' stata inviata nel rispetto della legge sulla privacy; se desidera maggiori informazioni o se intende cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/
It all depends: how busy you are, how expensive staff time in your country is and how costly the place is you buy your stubs from. Obviously some specimens should be stored in a desiccator for an eternity and other can be discarded immediately. There is nothing magical about cleaning stubs. Solvents are messy. Best to place on some newspaper a very coarse file or a sheet of coarse (50 grit) sandpaper and then rub the old stub over that file of sandpaper until specimen and glue are removed.
Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this leaves some money for useful purchases. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori [SMTP:sojilori-at-oauife.edu.ng] wrote: } } Hello Guys } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was } just installed in our central science lab. } Is there a way to clean stubs for reuse or are stubs disposable once used. } ( This may sound naive) } soji } Mr. O. O. ILORI } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING } OBAFEMI AWOLOWO UNIVERSITY, } ILE-IFE, OSUN STATE } NIGERIA. } email: sojilori-at-oauife.edu.ng } }
Dear Listers, The following are responses to my inquiry about automated critical point dryers. There was one other which I will post as well. Thanks to all who responded. Rosemary
I find automated CPDs easier only in the sense that they make training new users easier. But they also can encourage not paying sufficient attention to the drying instrument. I like the Polaron, in particular it's large chamber. Let's see ... the only other advantage to an automated system is if you have lots of samples to do in lots of runs. Otherwise, I've never seen enough of an advantage to justify the extra cost.
But! having said that, a CPD with autobleed is an excellent choice. This is a good safety feature, and can help protect specimens.
Phil
There are safety considerations, that is one of the several reasons why there is such a preference for the E3000 or E3100. BTW another good thing about the Polaron design and larger chamber is the less likelihood of turbulence, or putting it another way, you can do your exchanges at a faster rate because you can use larger flow rates before turbulence does set in.
Chuck (Garber)
We recently purchased a Tousimis 795 semi-automatic critical point dryer for our multi-user facilty. This unit replaces an old Denton "bomb". We considered ease of use for our customers to be an important consideration. Our unit has preset purge, bleed, vent and heating features which make it easier to use. We don't have to lug a large beaker of hot water over to the CPD to heat the chamber. The flow rates are factory preset but easily adjustable. Having these features, I believe, allows us to produce more consistent, reproducible results. The standard chamber size is rather small but a chamber extension is available and easy to install. We have found that when trying to critical point dry very large specimens, the chamber size can be a problem (most of our work is Drosophila flies or mouse embryos).
Although very few people do their own critical point drying here, our goal is to make the facility as user-friendly as possible, and this seemed to be a step in that direction.
Happy hunting.
Tom Januszewski Senior Electron Microscopist Molecular and Cellular Imaging Facility
Rosemary Walsh, Manager The Electron Microscope Facility for the Life Sciences, A Shared Technology Facility, The Life Sciences Consortium 1 South FrearLab Penn State University University Park, PA 16802 (814) 865-0212 rw9-at-psu.edu http://www.lsc.psu.edu/stf/em/home.html
i'd like to purchase a backscattered detector for an old Hitachi SEM. I am looking for vendors of such equipment, as Robinson detector, KE Si detector and so on. Could you help me with some addresses?
Thanks,
Peter
-- Dr NAGY Peter Chemical Research Center Institute of Chemistry of the Hungarian Academy of Sciences 1025 Budapest Pusztaszeri ut 59-67. Phone: 36-1-325-7548 Fax: 36-1-325-7509 E-mail: nagyp-at-chemres.hu
Dear Soji We usually treat the standard aluminium LEO pin stubs as disposable. Since we have a number of users each with a different specimen type and mounting procedure it becomes difficult to identify a single effective cleaning method, and staff time is more costly than the stubs.
If you are a novice user you may not yet realise that LEO pin stubs are obtainable at a fraction of LEO's price from most microscopy supply houses.
http://www.kaker.com/mvd/list.html is a list of microscopy vendors which you may find useful. Use this to look up web sites of some of the following companies: Agar Scientific, TAAB Laboratories, SPI, Electron Microscopy Science, etc. etc.
However, cleaning stubs can be cost-effective if you routinely produce many tens or hundreds of specimens in exactly the same way. If you have to do this it pays to find adhesives and conductive paints that redissolve readily in the same solvent, so things like epoxies are out. Choose your solvents carefully, because some like ethanol attack aluminium if it is immersed for long periods.
Another approach which avoids the need to clean stubs is to mount up your specimens on thin squares or discs of metal (e.g. thin aluminium sheet) which are then tacked to the stub using e.g. graphite dag. After use the metal supports can be cracked off and replaced with new ones. This way the stub can be re-used more often before it needs to be properly cleaned up. Good luck Chris
} On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori } [SMTP:sojilori-at-oauife.edu.ng] wrote: } } } } Hello Guys } } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was } } just installed in our central science lab. } } Is there a way to clean stubs for reuse or are stubs disposable once used. } } ( This may sound naive) } } soji } } Mr. O. O. ILORI } } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING } } OBAFEMI AWOLOWO UNIVERSITY, } } ILE-IFE, OSUN STATE } } NIGERIA. } } email: sojilori-at-oauife.edu.ng } } } } } }
========================================== Dr. Chris Jeffree University of Edinburgh BIOSEM - Biological Sciences Electron Microscope Facility Institute of Cell and Molecular Biology Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 (0) 131 650 5345 FAX. #44 (0) 131 650 6563 Mobile 07710 585 401 email c.jeffree-at-ed.ac.uk =========================================
We are considering a purchase of a system for quantitative analysis of bacterial and fungal biovolume in soil samples. Could anyone provide info on preferred method (FITC labeling?) and equipment. As always, I appreciate your help, Alice.
Alice Dohnalkova Environmental Microbiology Battelle, PNNL MS P7-50 Richland, WA 99352 tel. (509) 372-0692 fax (509) 376-1321
Ref: Diode detector type... I have used GW Electronics. First on an ETEC SEM, and now on a Hitachi S-3500. I started with a model (many years ago) which is now discontinued and have upgraded to the current design. Both worked well for me. Check: http://www.gwelectronics.com/
---------------------------------------- Woody White McDermott Technology, Inc. http://www.mtiresearch.com/
Peter Try looking at this site. http://www.kaker.com/mvd/list.html It contains links to dozens of microscopy vendor sites Chris
Date sent: Wed, 11 Oct 2000 08:02:17 -0500 To: Microscopy-at-sparc5.microscopy.com } From: Nagy Peter {nagyp-at-chemres.hu}
The last time this subject came up, someone suggested wiping the inside of the jar with Polaroid print coaters. They seem to provide a water soluble layer that easily comes off. We used to have dozens of extras lying around and now had a use for them. However, now that we have largely gone to digital imaging we will have to watch our stock.
At 04:49 PM 10/10/2000 -0700, you wrote:
} Well we've got a new vacuum evaporator and it has been so long since I've } had one I've forgotten how to treat the bell jar to reduce the effort it } takes to keep it clean. However, I do recall that there were little } tricks to cleaning and treating the glass and other surfaces. Anyone care } to shed a little light my darkened memory of these procedures?
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
We agree with John Nailon that if a user forgets to prepare the evaporator prior to a run he should have to clean it. Since we have to clean our evaporators several times a day because of our substrate production, we do the following.
1. Place Victawet in a medium basket. 2. Evaporate the Victawet at 10(-5). This coats not only the bell jar, but all the other surfaces under the bell jar as well. 3. Clean the system with windex (or water) and wipe with a lint free cloth. 4. Re-coat with victawet prior to the next run.
John Arnott
Disclaimer: In additon to using, Ladd has been selling most of the above mentioned products, including Victawet, Lint-free cloth, Substrates, Evaporators and Baskets since 1955. --
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
web site http://www.laddresearch.com
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail sales-at-laddresearch.com
Quality Since 1955
John Nailon wrote: } } ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.} } The easiest way to keep your bell jar clean is to wipe a thin film of } detergent over the surface of the clean glass and pump down to dry. } After each coating run simply rinse off the glass, the detergent comes } away and takes the coating with it. Recoat with detergent and dry. } } Train ALL users in this technique, insist they clean the glass if they } "FORGOT" to do it after their last run, and you will always have a } clear, clean bell jar. } Regards } JVN } Rick Harris wrote: } } } } ------------------------------------------------------------------------} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------.} } } } Well we've got a new vacuum evaporator and it has been so long since I've } } had one I've forgotten how to treat the bell jar to reduce the effort it } } takes to keep it clean. However, I do recall that there were little tricks } } to cleaning and treating the glass and other surfaces. Anyone care to shed } } a little light my darkened memory of these procedures? } } } } TIA } } } } Rick A. Harris, Director } } Microscopy and Imaging Facility } } Section of Molecular and Cellular Biology } } 1241 Life Sciences Addition } } University of California } } Davis, CA } } 530 752 2914 } } 530 754 7536 fax } } http://katie.ucdavis.edu } } raharris-at-ucdavis.edu } } -- } **************************************************** } John V Nailon } Operations Manager } Centre for Microscopy and Microanalysis } The University of Queensland } St. Lucia Queensland 4072 } Phone: +61-7-3365-4214 } Fax: +61-7-3365-4422 } WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon } ****************************************************
I need to have the phosphor screens recoated on an EM 400T we have on campus. I have been told that Grant Scientific does a good job. I have not been able to find a way to contact them. Can anyone help with a phone number or info.? I would also welcome any other suggestions. Thanks.
Joel McClintock EM Specialist Univ. of Kentucky Lexington, Ky 859-257-1242
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To minimize the area which needs cleaning, I obtained a pyrex glass cylinder large enough to fit vertically over my evaporating supports, etc. The ends of it were coated with a vacuum rated epoxy to prevent chipping. Before using, it is coated with a commercial release agent made for this "easy cleaning" purpose. The bottom sits on a sheet of disposable aluminum foil and the top is also covered by aluminum foil. To facilitate pumping, a 1/4" thick scrap of ceramic placed under the bottom edge of the "containment cylinder" aids pumping at a reasonable rate and provides a path for one of the power leads for my evaporating basket, insulating the lead from a metal support
platform. After use, the foil can be scrapped and only a 6" diameter cylinder requires cleaning!
Bernie Kestel Materials Science Division Argonne National Laboratory 9700 South Cass Avenue Argonne, Il., 60439
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id KAA08797 for dist-Microscopy; Wed, 11 Oct 2000 10:54:10 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id KAA08794 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Wed, 11 Oct 2000 10:53:39 -0500 (CDT) Received: from mail.interchange.ubc.ca (mail.interchange.ubc.ca [137.82.27.15]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id KAA08787 for {Microscopy-at-msa.microscopy.com} ; Wed, 11 Oct 2000 10:53:28 -0500 (CDT) Received: from sem1.staff.mmat.ubc.ca ([137.82.16.177] helo=sem1) by mail.interchange.ubc.ca with smtp (Exim 3.03 #1) id 13jOBR-0006nJ-00; Wed, 11 Oct 2000 08:52:45 -0700 Message-Id: {2.2.32.20001011155137.008fd974-at-pop.interchange.ubc.ca} X-Sender: mager-at-pop.interchange.ubc.ca X-Mailer: Windows Eudora Pro Version 2.2 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
Dear Rick, I used to use Victawet and it was the best, but it is not available, so I use a bar of hand soap. After you clean the bell jar or, if it is new, after you wet it, rub the dry bar of soap all over the inside and rub with a paper towel to spread it evenly and make it transparent. Good luck. At 04:49 PM 10/10/00 -0700, you wrote: } Well we've got a new vacuum evaporator and it has been so long since I've } had one I've forgotten how to treat the bell jar to reduce the effort it } takes to keep it clean. However, I do recall that there were little tricks } to cleaning and treating the glass and other surfaces. Anyone care to shed } a little light my darkened memory of these procedures? } } TIA } } Rick A. Harris, Director Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Dear Mr. Olori, The stubs used for SEM are reused for years, but the grids for a TEM are disposed of. I clean my SEM stubs by rubbing on a paper towel wet with either lab alcohol (ethanol) or acetone, depending what was on them. If I am using the carbon double-side sticky tabs, I soak the stubs in acetone for an hour, then rub on a paper towel to remove the tab. Congratulations on your new SEM. At 06:09 PM 10/10/00 +0100, you wrote: } } Hello Guys } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was } just installed in our central science lab. } Is there a way to clean stubs for reuse or are stubs disposable once used. } ( This may sound naive) } soji } Mr. O. O. ILORI } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING } OBAFEMI AWOLOWO UNIVERSITY, } ILE-IFE, OSUN STATE } NIGERIA. } email: sojilori-at-oauife.edu.ng
Good luck, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Peter Nagy wrote: ========================================================== i'd like to purchase a backscattered detector for an old Hitachi SEM. I am looking for vendors of such equipment, as Robinson detector, KE Si detector and so on. Could you help me with some addresses? =========================================================== Try Microscopy Vendor's Data Base http://207.137.96.185/mvd/products/back.html
They have them all, I think.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
I suppose that John Russ and others have stated the matter concisely and eloquently in their books and articles on the subject, but the concepts are not that difficult that I will take a stab at it.
A digital image is nothing more than a two-dimensional array of numbers where the numbers represent some characteristic of the image. Various image sources vary in the way that the arrays are assembled and what the numbers represent.
In an scanning electron microscope or a scanning probe microscope (which I suppose you could say that an SEM is), a single detector element is used to provide the signal at all points in the image. The X and Y coordinates of the scan are used to determine the array or pixel index, and the signal is digitized at each point.
Many systems utilize "active" control where the beam is directed to each coordinate in succession under computer control. A digital grid is predetermined within the computer and the coordinates are fed out through appropriate hardware to convert the digital coordinates to the necessary analog voltage to scan the probe to the desired point. A microscope signal is measured at that point and digitized for storage in the image array. The scan speed and resolution are under direct control of a computer.
Other systems are termed "passive" since the existing analog microscope scan system is used to control the probe scan as normal. The digitizing system monitors the x and y coordinates and records the signal level when the beam is in positions corresponding to the pixels in the image array.
There are a variety of signals available from electron microscopes. Detectors for secondary electrons and backscattered electrons operate as analog devices. (One might argue that since they are counting a finite number of electrons, then they could be setup as digital devices. However, the count rate is high enough that digital detectors are probably not feasible, and even if they were there remains a question of when to start counting.) A variety of detectors and devices are used (including PMTs - photo multiplier tubes) to measure the signal, but they are primarily, if not all, analog devices. An analog to digital convertor is used to convert the signal to digital for the image array. Some scopes may digitize the signal early on so that no analog signal is readily available, but it still exists somewhere inside the instrument.
X-ray signals in an electron microscope are probably treated as digital from the outset. Since the x-ray photons are detected as pulses from the x-ray detector, they are treated as discrete events and counted. Since counting is involved, x-ray signals are only used with active control systems (to the best of my knowledge). A counter is reset each time the probe is moved to a new location.
There is a whole other class of source for digital images. In this class there is a separate detector for each element of the array. The entire image is present at once as opposed to being built up by a scanned probe. The detectors operate in parallel as they build up and digitize the signal simultaneously. At some point the signal is read and the detectors are reset. CCD cameras are the most common device in this category. They find application in light microscopes and transmission electron microscopes as well as in consumer electronics such as video cameras and still cameras.
In these systems, the images are digital from the start. The readout is in terms of an electron count. It usually gets scaled to another digital range (0-255, 0-4095, etc) so that the original digital count may no longer be significant.
The size of the image array is determined by the size of the CCD array. Smaller image sizes can be obtained by grouping the signals from adjacent elements in the CCD array. Larger images are sometimes manufactured by interpolating the signals of neighboring elements, but that is not introducing new information.
I hope this helps. Feel free to ask for further clarification.
Warren S.
At 06:40 PM 10/10/2000 -0400, you wrote: } Dear listserv: } } Dee Breger was kind enough to forward this message for me. I'm writing a book } on digital photography, to be published by Harry N. Abrams next year. The book } will include a section on applied digital imaging, and I am particularly } interested in scientific imaging. I am hoping that some of you folks might } be able } to provide some information. } } For the purposes of the book, I am defining digital imaging as that which } describes a scene using discrete digital information in a raster grid, or } bitmap. So, I am particularly interested in scientific imaging applications } which gather information in this way. } } I would be interested to hear if there are other scientific imaging systems } (DEE: besides SEMs) which collect information in this manner. For instance, } it sounds as though atomic force microscopes work in this way: as they } drag a stylus across a surface, the position is recorded (x and y), along } with the height (z). This } is later used to re-create the image in a raster grid (the fact that the AFM } does not use light makes it another interesting foil to 'traditional' digital } photography). How then, are colors in the final output determined from the } data collected? I would also, of course, love to see any images you might have } to show. } } Thanks in advance, } Jonathan Lipkin (jlipkin-at-ramapo.edu) } } DEE: I'll forward to Jonathan any replies that are publically posted to the } listserver. He also needs other detailed technical information on digital } image formation which can be far more comprehensively clarified and } amplified by some of the experts among my fellow listers than by me, though } I've already given him the broad picture for analog scopes like my dear old } Cambridge 250. His SEM impressions, which I'll pass along verbatim, } include (1) "SEMs collect information, used to fill a raster grid, through } the use of a PMT", (2) the signal is sent directly to the crt in analog } form -I always had thought that the pmt sent out digital info, but it } sounds like it just sends out analog data (a waveform? though it couldn't } be a waveform if the raster grid is discrete in two dimensions)" and (3) } "as the secondary electrons come off the sample, they hit a detector which } generates a digital number (redundant) which is then placed in a pixel of a } bitmap." } } Many thanks to any who can help!
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
Grant does very fine work. As a mater of fact they are the source used by a few OEMs. Their address is 1385 Rock Island Road Gilbert South Carolina 29054 Phone: 803/892-2841
Regards,
Alex Greene SCIENTIFIC INSTRUMENTATION SERVICES, INC. PMB-499, 1807 West Slaughter Lane, Number 200 Austin, Texas 78748-6200 Phone: 512/282-5507 Fax: 512/280-0702
QUALITY ELECTRON MICROSCOPE REPAIR -----Original Message----- } From: Joel McClintock {jmcclin-at-pop.uky.edu} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
We slice off the specimen with a razor blade and use the blade to scrape the stub fairly clean. As we use colloidal graphite, which is conductive, I do not mind some residual glue as it will get covered by glue from the next use. This glue can be removed, if required, by sonicating in butyl acetate.
Dave
On Wed, 11 Oct 2000 21:09:07 +1000 Jim at ProSciTech {jim-at-proscitech.com} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } It all depends: how busy you are, how expensive staff time in your country is } and how costly the place is you buy your stubs from. Obviously some specimens } should be stored in a desiccator for an eternity and other can be discarded } immediately. } There is nothing magical about cleaning stubs. Solvents are messy. Best to } place on some newspaper a very coarse file or a sheet of coarse (50 grit) } sandpaper and then rub the old stub over that file of sandpaper until specimen } and glue are removed. } } Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this } leaves some money for useful purchases. } Cheers } Jim Darley } ProSciTech Microscopy PLUS } PO Box 111, Thuringowa QLD 4817 Australia } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com } Great microscopy catalogue, 500 Links, MSDS, User Notes } ABN: 99 724 136 560 www.proscitech.com } } On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori } [SMTP:sojilori-at-oauife.edu.ng] wrote: } } } } Hello Guys } } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was } } just installed in our central science lab. } } Is there a way to clean stubs for reuse or are stubs disposable once used. } } ( This may sound naive) } } soji } } Mr. O. O. ILORI } } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING } } OBAFEMI AWOLOWO UNIVERSITY, } } ILE-IFE, OSUN STATE } } NIGERIA. } } email: sojilori-at-oauife.edu.ng } } } } } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
I need to have the phosphor screens recoated on an EM 400T we have on campus. I have been told that Grant Scientific does a good job. I have not been able to find a way to contact them. Can anyone help with a phone number or info.? I would also welcome any other suggestions. Thanks. Dear Joel, (803)892-2841. I just tried it, and it works. I also think that Grant did a good job for us. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
Hi List, This is a question/situation a colleague of mine wanted to post-her email for replies not on the list is rhealy-at-iastate.edu. Of all people out there, I know you all can help! Thanks so much! Tracey Pepper
We are working with Cochliobolus inoculated maize leaf tissue using jet-propane fixation, and freeze substitution using acetone (for immuno resins we switch to ultra pure ETOH prior to infiltration), and have attempted using several immuno-type resins such as: Lowicryl HM20 (with poly. at -20 under UV), LR White with accel. at 4deg., and Quetol for general morphology. Our ultimate goal is to visualize extra-cellular matrix of fungus within leaf tissues using immunocytochem. So far, fixation is great (general morph.) However, the immuno resins are not infiltrating adequately (under -30 deg.for Lowicryl and 4 deg. for LR White), lots of separation, and shattering. The longest we have infiltrated has been 1-2 hr. each step for LR White. The Lowicryl had an overnight in pure followed by 1/2 day for each of five total changes. Any and all suggestions would be greatly appreciated! Regards, Rosanne Healy
Tracey Pepper Supervisor Bessey Microscopy Facility Iowa State University ph: 515-294-3872 fax: 515.294.1337
Ben Craft wrote: ============================================================= I'm getting ready to purchase a new sputter coater for our FESEM. I'd like to hear how lucky others were with using a "regular" sputter coater for high resolution SEM work. Currently, there is a noise problem with our SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm ZrO2 close-packed particles, and 4nm pores in glass using a thin coating of Au/Pd applied with an old Polaron. One thing I'm worried about is = once the noise is fixed and I can resolve the published 1.5nm resolution will I be able to resolve the grains in the coating? ============================================================= One system to consider is the Osmium Plasma Coater OPC-60 made by Nippon Laser and Electronics, Nagoya, Japan. It is distributed by SPI Supplies. You can find extensive information and examples on URL http://www.2spi.com/catalog/osmi-coat.html
The end result is an amorphous coating so there is no grain size with which one has to contend. The source of the osmium is an ampoule of osmium tetroxide. This is not just a simple matter of sputtering from an osmium metal cathode. And since the coating is an inert precious group metal, its shelf life is quite long, if not infinite. Safety concerns are address on the webpage mentioned above.
Contact me off-line should you want us to do a demo coating for you.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
We just bought a Pelco CPD2 with the hopes of replacing our old Polaron E3000, and so far, we're rather unimpressed just trying to get it through an initial setup. We were wondering if any other users could tell us how many runs they get out of an average CO2 cylinder? The cooling process with the CO2 seems somewhat inefficient at this point and we're using up a lot just trying to cool the chamber. Also any recommendations on the soak vs. the rinse methods of infiltrating the samples would be appreciated. I can only assume the operations get a little easier with practise, but right now we're wondering whether it's worth the investment in the long run. The Polaron has been very reliable and has served us well, but we're worried that its age is compromising its safety.
Our microscopy facility recently merged with the scientific photography and graphics production unit on campus. We need to quickly evaluate the potential value of a large format inkjet printer for producing posters for scientific meetings.
We welcome comments, suggestions and especially personal experiences using inkjet technology for poster production. Is it worth the expense, do researchers use it, etc... We are looking at the Epson 9000 printer in combination with the Fujifilm digital 9000 Photo Graphix RIP. Good idea? How about software for poster production?
Also, vendors are welcome to contact me and to submit quotations. We need all of this information by Friday (Oct 13). Sorry for the rush but I just now got the word that funds may be available. Whew....
Many thanks....
John B.
-- #################################################################### John J. Bozzola, Ph.D., Director IMAGE (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
Victawet is a stock item for Ladd. We sell and use it on a daily basis. Please let me know off-line if you want a quote.
Thanks,
JD Arnott
--
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
web site http://www.laddresearch.com
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail sales-at-laddresearch.com
Quality Since 1955 Mary Mager wrote: } } ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.} } Dear Rick, } I used to use Victawet and it was the best, but it is not available, so I } use a bar of hand soap. After you clean the bell jar or, if it is new, after } you wet it, rub the dry bar of soap all over the inside and rub with a paper } towel to spread it evenly and make it transparent. Good luck. } At 04:49 PM 10/10/00 -0700, you wrote: } } Well we've got a new vacuum evaporator and it has been so long since I've } } had one I've forgotten how to treat the bell jar to reduce the effort it } } takes to keep it clean. However, I do recall that there were little tricks } } to cleaning and treating the glass and other surfaces. Anyone care to shed } } a little light my darkened memory of these procedures? } } } } TIA } } } } Rick A. Harris, Director } Regards, } Mary } } Mary Mager } Electron Microscopist } Metals and Materials Engineering } University of British Columbia } 6350 Stores Road } Vancouver, B.C. V6T 1Z4 } CANADA } tel: 604-822-5648 } e-mail: mager-at-interchg.ubc.ca
GRANT SCIENTIFIC CORP 1385 ROCK ISLAND ROAD GILBERT , SC 29054 803-892-2841 PHONE 803-892-2441 FAX
} Listers, } } I need to have the phosphor screens recoated on an EM 400T we have on } campus. I have been told that Grant Scientific does a good job. I have not } been able to find a way to contact them. Can anyone help with a phone number } or info.? I would also welcome any other suggestions. Thanks. } } Joel McClintock } EM Specialist } Univ. of Kentucky } Lexington, Ky } 859-257-1242
We are moving out our Philips EM400T TEM. It has suffered from vacuum logic and lens logic problems -- problems that currently mean that it is unusable. It has a STEM unit, a Quantum EDX detector, Kevex 7000 system, and a variety of holders, including tilt-rotate. Anyone interested in some or all of this equipment should get in touch ASAP, please. Thanks, Brian Robertson
*********************************************************** Assoc. Prof. Brian W. Robertson Department of Mechanical Engineering and Center for Materials Research and Analysis University of Nebraska-Lincoln, N124 WSEC, 17th & Vine Sts., Lincoln, NE 68588-0656, USA ** Office 402 472 8308; FAX 472 1465; Labs 472 8762 and 472 5498 **
I have a Reichert Jung Ultracut S/N382797, Type701701, acquired from Upjohn Japan. The unit has no stereoscope or power unit (lost in packaging or shipping). Any person interested in the unit to use or for parts, please don't hesitate to contact me.
Pauline Yu wrote: =========================================================== ........................ The Polaron has been very reliable and has served us well, but we're worried that its age is compromising its safety. ========================================================== Unless the unit has been dropped or otherwise abused, it is difficult for me to see how its "age" could have anything to do with its safety. But if this was your concern, there is a process, and we could help you arrange it, by which the chamber is returned to the UK, and retested in the same laboratory where it was tested originally, and essentially recertified. The testing is done at a pressure that is far beyond where a rupture disc would blow, so you could then use it with confidence for many years to come. The cost of the service is far less than the cost of a new system.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
I experimented with several different cleaning/mounting procedures and the one that is the most time effective for me is the following: I apply a conductive silicone release agent to my stubs before I apply carbon double stick tape to them, then I use a conductive Al or Cu tape ( I cut it in half length-wise so it doesn't take up so much space ) to insure conductivity, works for my samples which are mostly dry powders, industrial hygiene dusts, inks and papers. Then when I want to clean the stubs I just lift the tape off with a knife ( not to sharp ) and rinse and rub any residue off with paper towels using a 50/50 % toluene / acetone mix, then ethanol in a ultrasonic cleaner if I need them really clean.
Terry Ellis Hallmark Cards Inc. tellis2-at-hallmark.com USA.
Listers, I meet some problem how to electrical-polish 3mm pure copper disk. Would you plese tell me the good solution and the good condition of electrical-polishing copper on tenupol-5 machine. Thanks
Mr.Zhongwen Yao EPFL- CRPP Villingen, PSI Switzerland
You may also want to consider the option of Chromium coating which will give grain siz of less than 0.5NM Chromium grain size with thin film deposition typically 5NM.
We manufacture and sell the K575 Sputter Coater unit. It has the advantage of automated cycle coating Turbomolecular drag pumping for High Vaccum.
Please take a look at our web site for a detailed technical brief and the instrument specification.
HTTP://www.emitech.co.uk
Kind regards
Gareth } -----Original Message----- } From: Garber, Charles A. [SMTP:cgarber-at-2spi.com] } Sent: Wednesday, October 11, 2000 7:40 PM } To: MICROSCOPY BB } Subject: Coater for use with FESEM } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } Ben Craft wrote: } ============================================================= } I'm getting ready to purchase a new sputter coater for our FESEM. I'd } like to hear how lucky others were with using a "regular" sputter } coater } for high resolution SEM work. Currently, there is a noise problem } with our } SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm } ZrO2 } close-packed particles, and 4nm pores in glass using a thin coating of } Au/Pd } applied with an old Polaron. One thing I'm worried about is = once the } noise } is fixed and I can resolve the published 1.5nm resolution will I be } able to } resolve the grains in the coating? } ============================================================= } One system to consider is the Osmium Plasma Coater OPC-60 made by } Nippon } Laser and Electronics, Nagoya, Japan. It is distributed by SPI } Supplies. } You can find extensive information and examples on URL } http://www.2spi.com/catalog/osmi-coat.html } } The end result is an amorphous coating so there is no grain size with } which } one has to contend. The source of the osmium is an ampoule of osmium } tetroxide. This is not just a simple matter of sputtering from an } osmium } metal cathode. And since the coating is an inert precious group } metal, its } shelf life is quite long, if not infinite. Safety concerns are } address on } the webpage mentioned above. } } Contact me off-line should you want us to do a demo coating for you. } } Chuck } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President 1-800-2424-SPI } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.spi.cc } ######################## } ============================================
I am looking for help concerning the following problem: Someone in our institute is trying to deposit a 10nm Au-film on a Ge single crystal by thermal evaporation from a tungsten boat (...rate: 0.5A/s). The main point to me is, if FESEM is an appropriate method to determine if we have really a Au-film or only gold islands ...
I do appreciate any comments concering both points (FESEM-tricks, because 99% of our samples are uncoated insulators) and the depostition technique/parameters for very thin gold films ...
I am looking for help concerning the following problem: Someone in our institute is trying to deposit a 10nm Au-film on a Ge single crystal by thermal evaporation from a tungsten boat (...rate: 0.5A/s). The main point to me is, if FESEM is an appropriate method to determine if we have really a Au-film or only gold islands ...
I do appreciate any comments concering both points (FESEM-tricks, because 99% of our samples are uncoated insulators) and the depostition technique/parameters for very thin gold films ...
Greetings, As seen below, Gareth Robinson mentions chromium as an option for fine coats to use in high res SEM.
A word of caution: Chromium is tricky to work with. You cannot use chromium sputter coating the same simply way you are used to sputtering with, say, gold or platinum. You have to keep the atmosphere in the coater squeaky clean, and even cleaner, and you must absolutely minimize the exposure of your coated sample to oxygen.
I expect that chromium coats can be superb, but you cannot expect to simply put a chromium target in your sputter coater and have decent results. We found this out the hard way.
As ever, Tobias
} } } } Ben, } } You may also want to consider the option of Chromium coating which will } give grain siz of less than 0.5NM Chromium grain size with thin film } deposition typically 5NM. } } We manufacture and sell the K575 Sputter Coater unit. It has the } advantage of automated cycle coating Turbomolecular drag pumping for } High Vaccum. } } Please take a look at our web site for a detailed technical brief and } the instrument specification. } } HTTP://www.emitech.co.uk } } Kind regards } } Gareth Gareth.Robinson-at-emitech.co.uk
At 5:38 PM -0500 10/11/00, Brian W Robertson wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Brian,
I am interested in the holders.
Regards, Lucille Giannuzzi
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Mechanical, Materials, and Aerospace Engineering Director, UCF/Cirent Materials Characterization Facility University of Central Florida 12443 Research Parkway, Suite 305 Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
In upgrading our light microscopes, we are buying some new lenses for our confocal scope and a brightfield scope. The salesforce is regaling us with tales of improved resolution, clarity, brightness etc that comes with spending double or triple the price for a standard oil immersion or dry lens. Could someone please clarify, either on-line or off, what the advantages and disadvantages of each of the following ( my texts on light microscopy don't deal with them). Are they worth the extra dollars?
1) a water immersion lens for looking at cells suspended in buffer and covered with a coverslip (water is used rather than oil between coverslip and lens) These lenses typically have a lower numerical aperture than an oil immersion lens.
2) a water dipping lens, used to examine cells in culture directly, without a coverslip. I would expect the absence of refractive indice changes (media/coverslip/air or oil) would explain their improved brightness.
As you are so close by to our San Clemente, CA lab, I would suggest that you come by and bring some samples to try on our IBS/e Ion Beam Sputter Deposition and Etching System.
The IBS/e, a thin film deposition and etching system, is designed to improve high resolution electron microscopy imaging by depositing ultra-thin, fine grain metal and carbon films on specimens. Even low voltage imaging is improved.
We can deposit many different metals and carbon or show you examples of contrast enhancement on various types of specimens from our library of micrographs. I will send to you by separate mail some AFM images comparing evaporated coatings, sputtered coatings and ion beam deposited films.
DISCLAIMER: South Bay Technology produces equipment and supplies as described above and, therefore, has a vested interest in promoting their use.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by Ben Craft } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I'm getting ready to purchase a new sputter coater for our FESEM. I'd like to hear how lucky others were with using a "regular" sputter coater for high resolution SEM work. Currently, there is a noise problem with our SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm ZrO2 close-packed particles, and 4nm pores in glass using a thin coating of Au/Pd applied with an old Polaron. One thing I'm worried about is = once the noise is fixed and I can resolve the published 1.5nm resolution will I be able to resolve the grains in the coating?
The 2000 Fall Meeting of the Texas Society for Microscopy will be held
on October 26, 27, and 28 at the DoubleTree Hotel in Dallas, TX. The hotel is located in Campbell Centre at 8350 N. Central Expressway, Dallas, TX 75206. The phone number for the hotel is 800-222-TREE. A special workshop will be held on Thursday, Oct. 26, entitled "The SEM Today". It will cover the latest information concerning basic SEM, LVSEM, ESEM and detectors. Dr. David Joy, University of Tennessee, will be speaking on Low Voltage SEM and Variable Pressure SEM. YOU MUST BE REGISTERED FOR THE THURSDAY SEMINAR SERIES. It is free for members and new member applicants. If you plan to attend and have not registered, call or email Pam Neill at 817-568-6497 or pamela.neill-at-alconlabs.com
TSM PROGRAM SUMMARY OF DAILY ACTIVITIES
Thursday October 26, 2000 8:30-9:00 Registration at TI 9:00-5:00 Workshop coordinated by Kevin Cronyn, Hitachi and hosted by TI 12:30-7:00 Exhibitor setup and Poster setup DoubleTree 5:30-7:00 TSM Executive council Meeting, DoubleTree 7:00-9:00 Evening Social, DoubleTree 9:30-11:00 TSM Hospitality room DoubleTree
Friday October 27, 2000 8:00-8:30 Continental Breakfast in vendor's room 8:00-11:00 Registration 8:30-8:45 Introduction and announcements. 8:45-10:00 Platform presentations 10:00-10:15 Break in exhibitors’ room. 10:15-11:30 Guest speaker Russ Pinazotto "Electron Microscopy of Semiconductor Nanoparticles" 11:45-1:45 Lunch and business meeting with vendors at DoubleTree Hotel Campbell Center 1:45-3:00 Guest speaker Dr. Charles Mims “Use of High Pressure Freezing for Biological TEM". He will also include some information on plunge freezing. 3:00-4:30 Break in exhibitors’ room. Poster authors should be at their posts 5:30-7:00 Social in the hospitality suite. Dinner on your own. There will be a trip planned to the West End via DART for dinner and entertainment.
Saturday October 28, 2000 8:00-9:00 Registration 8:00-9:00 Continental Breakfast 8:00-9:15 Poster session continued 9:30-10:45 Platform presentations 10:45 adjourn
For more information and/or to register, please contact Pamela Neill, Program Chair TSM Alcon Research LTD 6201 South Freeway Forth Worth, TX 76134-2099 pamela.neill-at-alconlabs.com 817-568-6497 -- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-598-1291 (pager) KFAB Physical Analysis Lab--SEM/FIB/FA Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
a) 33% nitric acid, 67% methanol cooled to -25C and at 1.5 V
b) 45% phosphoric acid, 55% water at { 10C (no voltage given)
c) 10% orthophosphoric, 90% water at 0C and at 8 V.
I had success with the first solution in a Tenupol more years ago than I care to contemplate!
Tom Malis Group Leader - Characterization Materials Technology Laboratory Natural Resources Canada (Govt. of Canada) 568 Booth St., Ottawa, Canada ph. 613-992-2310 FAX 613-992-8735 email: malis-at-nrcan.gc.ca
---------- From: Zhongwen Yao [SMTP:zong-wen.yao-at-psi.ch] Sent: Thursday, October 12, 2000 9:02 AM To: Microscopy-at-sparc5.microscopy.com Subject: copper eclectrical polishing
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Listers, I meet some problem how to electrical-polish 3mm pure copper disk. Would you plese tell me the good solution and the good condition of electrical-polishing copper on tenupol-5 machine. Thanks
Mr.Zhongwen Yao EPFL- CRPP Villingen, PSI Switzerland
} Netters } } In upgrading our light microscopes, we are buying some new lenses for our } confocal scope and a brightfield scope. The salesforce is regaling us with } tales of improved resolution, clarity, brightness etc that comes with } spending double or triple the price for a standard oil immersion or dry } lens. Could someone please clarify, either on-line or off, what the } advantages and disadvantages of each of the following ( my texts on light } microscopy don't deal with them). Are they worth the extra dollars? } } 1) a water immersion lens for looking at cells suspended in buffer and } covered with a coverslip (water is used rather than oil between coverslip } and lens) These lenses typically have a lower numerical aperture than an } oil immersion lens. } } 2) a water dipping lens, used to examine cells in culture directly, without } a coverslip. I would expect the absence of refractive indice changes } (media/coverslip/air or oil) would explain their improved brightness. } } Thanks in advance for your input } } Steve Barlow } SDSU EM Facility
So they are saying that all of their old lenses are junk? Ask for a demo using your material at your site. Then you can see for yourself
Geoff
-- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
Responding to the message of {Pine.GSO.4.10.10010111120030.19238-100000-at-aggie.pw.usda.gov} from "Pauline C. Yu" {splene-at-pw.usda.gov} : } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.
} Hi listers } } We just bought a Pelco CPD2 with the hopes of replacing our old Polaron } E3000, and so far, we're rather unimpressed just trying to get it through } an initial setup. } We were wondering if any other users could tell us how many runs they get } out of an average CO2 cylinder? The cooling process with the CO2 seems } somewhat inefficient at this point and we're using up a lot just trying to } cool the chamber.
Pauline,
You should get very many runs out of an average cylinder. I'm not sure how the Pelco is designed, but with my Ladd Company critical point dryer, also cooled by CO2 gas flow-through, the "trick" is to set up a balance between the CO2 input valve and the exhaust or drain valve such that you get a BIG pressure drop from the CO2 tank pressure which is about 1000 psi - down to about 100-200 psi in the chamber as the gas flows through. You shouldn't need to open the input valve very much to achieve this effect. The cooling of the gas, hence the chamber, depends on a big pressure drop, the ol' Joule-Thomposon effect, just like in a refrigerator. When I first got my Ladd unit I had the same problem because I had too high a pressure in the chamber during blow through of the gas.
I hope this helps. It should only take just a few minutes to cool the chamber from room temp to about 5 C, for example, depending on size and mass of the chamber on your unit
Gib Ahlstrand Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu http://biosci.umn.edu/MIC/consortium.html
I have looked in our archives and have found 2 solutions that Bernie Kestel from Argonne National Lab has developed for copper which may be of use. This work was done with our Model 550 single vertical jet electropolisher so references to jet height would be unique to our system. The other parameters may need to be adjusted slightly. Please be careful in using any of these chemicals and especially when adjusting the recipes. I owuld suggest that you contact Bernie Kestel directly prior to using these. You can reach him by email at kestel-at-anl.gov.
Recipe #2 150 ml HNO3 350 ml methanol 40 ml butyl cellosolve
Temp: -20 degrees C Jet height: 3.9mm Pump setting: 2.5 Volts: 40 Current: 75mA NOTES: excellent polish. Lower temperature result in an uneven foil surface.
I hope this information helps. If I can be of any additional assistance, please feel free to contact me.
DISCLAIMER: South Bay Technology produces equipment and supplies as described above and, therefore, has a vested interest in promoting their use.
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by Zhongwen Yao } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Listers, I meet some problem how to electrical-polish 3mm pure copper disk. Would you plese tell me the good solution and the good condition of electrical-polishing copper on tenupol-5 machine. Thanks
Mr.Zhongwen Yao EPFL- CRPP Villingen, PSI Switzerland
The K575 uses rotary and turbomolecular drag pumping to achive the high vaccum required.
The instrument has an automated shutter assembly to allow sputter target cleaning (Remove the oxide layer from the chromium target material) prior to sample sputtering.
We ahve had some very good application results witht he coater, please drop me a line for further info.
Best regards
Gareth } -----Original Message----- } From: Tobias Baskin [SMTP:BaskinT-at-missouri.edu] } Sent: Thursday, October 12, 2000 3:00 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: RE: Coater for use with FESEM } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } Greetings, } As seen below, Gareth Robinson mentions chromium as an option } for fine coats to use in high res SEM. } } A word of caution: Chromium is tricky to work with. You } cannot use chromium sputter coating the same simply way you are used } to sputtering with, say, gold or platinum. You have to keep the } atmosphere in the coater squeaky clean, and even cleaner, and you } must absolutely minimize the exposure of your coated sample to oxygen. } } I expect that chromium coats can be superb, but you cannot } expect to simply put a chromium target in your sputter coater and } have decent results. We found this out the hard way. } } As ever, } Tobias } } } } } } } } } Ben, } } } } You may also want to consider the option of Chromium coating which } will } } give grain siz of less than 0.5NM Chromium grain size with thin film } } deposition typically 5NM. } } } } We manufacture and sell the K575 Sputter Coater unit. It has the } } advantage of automated cycle coating Turbomolecular drag pumping for } } High Vaccum. } } } } Please take a look at our web site for a detailed technical brief and } } the instrument specification. } } } } HTTP://www.emitech.co.uk } } } } Kind regards } } } } Gareth Gareth.Robinson-at-emitech.co.uk } } -- } _ ____ __ ____ Tobias I. Baskin } / \ / / \ / \ \ 109 Tucker Hall } / / / / \ \ \ Biological } Sciences } /_ / __ /__ \ \ \__ University of } Missouri } / / / \ \ \ Columbia, } MO USA } / / / \ \ \ 65211-7400 } / / ___ / \ \__/ \ ____ voice: } 573-882-0173 } fax: 573-882-0123
Hi All, I have a JSM 840 that I need to relocate soon. The new location is very near a railroad and interstate! Does anyone have information on the optimum thickness of a concrete slab to prevent vibrations? Thanks,
Steve Buckingham Excellatron Solid State LLC 1460 Roswell Street, Suite J Smyrna, GA 30080 phone 770 438 2201 fax 770 438 2118
Hi E-beam buddies, After our last excimer laser was shut down, I had the idea of cleaning our Pt apertures with our plasma etcher using dry air. Optically they appear cleaner but in the TEM they are worse than before they went into the plasma. The aperture ID edge appears to be populated with hemispherical features that are prone to charging. I considered back streaming from the fomblin filled mech. pump but there is no residual smell of oil or sign of it anywhere about the chamber. Does anyone have any idea what is going on & perhaps how these apertures might be recovered? Some are cheap some are not.
Hi All, My goal is to localize active oxygen species/peroxidase activity in roots, and am wondering if anyone out there has any advice. I have several protocols, some using very different ways of utilizing DAB, and some using cerium chloride. They range from using whole mount tissue to processing tissue for TEM (and actually nothing in between). I am currently trying one of the DAB protocols, but would greatly appreciate hearing from anyone who has some insight. Thanks again for your help, Kristen Kristen A. Lennon Department of Plant Pathology 351 Bessey Hall Iowa State University Ames, IA 50011 kalen-at-iastate.edu
Funny thing is, even some really old lenses (old brass ones)! When it comes to low power still work pretty good!
I think many of the newer faster better sometimes what we will notice is very slight, but sales forces tend to extol the fact we need the latest even when perhaps we don't. i think anything produced today will only be just a little bit if at all better than the stuff form the 80's
Now on electron microscopes things are a bunch diff. with age especially on that old rust bucket of the edax computer We have on the AMR 1000... I suppose I should gather some spares or just consider replacing it with a PC when we finally get it set up and operational.
Ed Sharpe archivist for SMECC
{ { Subj: Re: light microscope lenses Date: 10/12/00 12:21:14 PM US Mountain Standard Time From: mcauliff-at-UMDNJ.EDU (Geoff McAuliffe) To: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow) CC: CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU, microscopy-at-sparc5.microscopy.com
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Steve Barlow wrote:
} Netters } } In upgrading our light microscopes, we are buying some new lenses for our } confocal scope and a brightfield scope. The salesforce is regaling us with } tales of improved resolution, clarity, brightness etc that comes with } spending double or triple the price for a standard oil immersion or dry } lens. Could someone please clarify, either on-line or off, what the } advantages and disadvantages of each of the following ( my texts on light } microscopy don't deal with them). Are they worth the extra dollars? } } 1) a water immersion lens for looking at cells suspended in buffer and } covered with a coverslip (water is used rather than oil between coverslip } and lens) These lenses typically have a lower numerical aperture than an } oil immersion lens. } } 2) a water dipping lens, used to examine cells in culture directly, without } a coverslip. I would expect the absence of refractive indice changes } (media/coverslip/air or oil) would explain their improved brightness. } } Thanks in advance for your input } } Steve Barlow } SDSU EM Facility
So they are saying that all of their old lenses are junk? Ask for a demo using your material at your site. Then you can see for yourself
Geoff
-- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
Dear Mr. Yao, The Struers Tenupol instructions I have recommend a solution for Cu polishing consisting of: 250 ml phosphoric acid 500 ml distilled water 250 ml ethanol 2 ml Vogel's Sparbeize 5 g urea "to be mixed successively" and used at 15 degrees C, 0.3 A current, 15 V, flowrate 4 for 1 minute. I'm afraid I have no idea what "Vogel's Sparbeize" is. Another solution I have used for the window technique is 33% (v/v) nitric acid and 67% methanol at room temperature ( {25 degrees C), 8 V. You must remove the sample quickly to prevent oxidation and wash in methanol. This is a very flammable and reactive mixture used as jet fuel, so keep it away from heat, sparks and flame. 50 ml At 08:02 AM 10/12/00 -0500, you wrote: } } Listers, I meet some problem how to electrical-polish 3mm pure copper } disk. Would you plese tell me the good solution and the good condition } of electrical-polishing copper on tenupol-5 machine. } Thanks } } Mr.Zhongwen Yao
Good luck, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Dear Gunnar, I would recommend masking a part of the Ge by putting a bit of Al foil over it before coating. You should be able to see the interface between the coated and uncoated surface clearly in the FESEM due to the atomic weight difference, and then zoom up to high magnification to see if there is any structure in the Au film. I know in a Au sputtered film this could be seen easily, but I haven't looked at a thermal evaporated film. It will show up best on a polished Ge wafer. You can use higher kV (15-20) and high resolution conditions in the FESEM, because the sample is conductive. At 03:58 PM 10/12/00 +0200, you wrote: } Hi, } } I am looking for help concerning the following problem: Someone in our } institute is trying to deposit a 10nm Au-film on a Ge single crystal by } thermal evaporation from a tungsten boat (...rate: 0.5A/s). } The main point to me is, if FESEM is an appropriate method to determine if } we } have really a Au-film or only gold islands ... } } I do appreciate any comments concering both points (FESEM-tricks, } because 99% of our samples are uncoated insulators) and the depostition } technique/parameters for very thin gold films ... } } ThanX very much in advance! } } } } Dipl.Ing.(FH) G.Glasser
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
MSA Listers, This is a long shot, but if anyone of you has information on the pin output of a DT2255 NuBus frame grabber and could forward to me off-list, I would be very grateful. DT does not have this info on their web page and seems to be selling support; I don't want to know how much they charge for pin output info on obsolete boards! Thanks.
-- Gregory Mulhollan, Ph.D. Extreme Devices Inc. 101 West 6th Street Suite 200 Austin, TX 78701 (512)479-7740 x2231 voice (512)479-7750 fax mulhollan-at-extremedevices.com
I would appreciate any advice/recommendations on TEM analysis of lipsomes, particularly vesicle size. Thanks Theresa
Theresa A. Fassel Core EM Unit, Mail Box MB-32 The Scripps Research Institute 10,550 N. Torrey Pines Rd. LaJolla, CA 92037-1027 tel: (858)-784-8182 fax: (858)-784-8193 tfassel-at-scripps.edu
Gatan Inc. has an immediate opening for a person to handle GIF and PEELS Product management. Gatan has a complete facility equiped with a new FEI Tecnai 20, a JEOL 2010/FasTEM and a Philips CM12.
The Individual should have either GIF or PEELS experience, a strong TEM background in Biological or Materials science, good computer skills, a willingness to travel and the vision and drive to help determine the future of these products.
The position is based in Pleasanton, CA and carries a salary comensurate with experience as well as a bonus plan and Corporate success share plan.
Please contact Ian Cotton, Director of Marketing at 925-224-7343 or email your resume to icotton-at-gatan.com.
********************************* Ian Cotton Marketing Director, Gatan, Inc. 5933 Coronado Lane Pleasanton, CA 94588 USA Phone 925-224-7343 Fax 925-463-0204 E-mail icotton-at-gatan.com Surf the Web to www.gatan.com *********************************
Gatan Inc. has an immediate opening for a person to manage our newly equiped demonstration facility. We have recently installed a new FEI Tecnai 20 and a JEOL 2010/FasTEM TEM. A full suite of Gatan analytical systems ( GIF and ENFINA PEELS ) and imaging systems are installed on these two microscopes.
The Individual should have either GIF or PEELS experience, a strong TEM background in Biological or Materials science, good computer skills, and excellent interpersonal skills. You should be experienced in the management of TEM instrumentation and have good organisational skills.
This is a challenging position for an individual who wants to work with state of the art equipment and contribute to the future development of our products.
The position is based in Pleasanton, CA and carries a salary comensurate with experience as well as a bonus plan and Corporate success share plan.
Please contact Ian Cotton, Director of Marketing at 925-224-7343 or email your resume to icotton-at-gatan.com.
********************************* Ian Cotton Marketing Director, Gatan, Inc. 5933 Coronado Lane Pleasanton, CA 94588 USA Phone 925-224-7343 Fax 925-463-0204 E-mail icotton-at-gatan.com Surf the Web to www.gatan.com *********************************
A colleague of mine is interested in purchasing a tissue processor. She has limited access to email so please reply directly to me and let me know what processor you use and would recommend. I'll pass the word along.
Thank you,
Ruth
*************************************** Ruth Yamawaki Department of Comparative Medicine Stanford University Stanford, CA 94305 ***************************************
*Date sent: Thu, 12 Oct 2000 08:02:18 -0500 *To: Microscopy-at-sparc5.microscopy.com *From: Zhongwen Yao {zong-wen.yao-at-psi.ch} *Subject: copper eclectrical polishing
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It seems that the best way, if you have enough money, is to buy proper electrolyte from the Struers. Based on our experiance, copper is difficult for TEM preparation. We use to say that even weather has an influance on electropolishing conditions. Good luck, Witold Zielinski
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% Witold Zielinski, Ph.D. Warsaw University of Technology Department of Materials Science and Engineering 02-507 Warszawa, Woloska 141 POLAND
Patton, David wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } We slice off the specimen with a razor blade and use the } blade to scrape the stub fairly clean. As we use colloidal } graphite, which is conductive, I do not mind some residual } glue as it will get covered by glue from the next use. } This glue can be removed, if required, by sonicating in } butyl acetate. } } Dave } } On Wed, 11 Oct 2000 21:09:07 +1000 Jim at ProSciTech } {jim-at-proscitech.com} wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } It all depends: how busy you are, how expensive staff time in your country is } } and how costly the place is you buy your stubs from. Obviously some specimens } } should be stored in a desiccator for an eternity and other can be discarded } } immediately. } } There is nothing magical about cleaning stubs. Solvents are messy. Best to } } place on some newspaper a very coarse file or a sheet of coarse (50 grit) } } sandpaper and then rub the old stub over that file of sandpaper until specimen } } and glue are removed. } } } } Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this } } leaves some money for useful purchases. } } Cheers } } Jim Darley } } ProSciTech Microscopy PLUS } } PO Box 111, Thuringowa QLD 4817 Australia } } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com } } Great microscopy catalogue, 500 Links, MSDS, User Notes } } ABN: 99 724 136 560 www.proscitech.com } } } } On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori } } [SMTP:sojilori-at-oauife.edu.ng] wrote: } } } } } } Hello Guys } } } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was } } } just installed in our central science lab. } } } Is there a way to clean stubs for reuse or are stubs disposable once used. } } } ( This may sound naive) } } } soji } } } Mr. O. O. ILORI } } } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING } } } OBAFEMI AWOLOWO UNIVERSITY, } } } ILE-IFE, OSUN STATE } } } NIGERIA. } } } email: sojilori-at-oauife.edu.ng } } } } } } } } } } } } ---------------------------------------- } Patton, David } Email: David.Patton-at-uwe.ac.uk } "University of the West of England" If you have access to a glass bead blaster, it does a great job as long as you weren't looking for a mirror finish.
Ken Converse owner Quality Images third party SEM service Delta, PA
Bruce Brinson wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi E-beam buddies, } After our last excimer laser was shut down, I had the idea of } cleaning our Pt apertures with our plasma etcher using dry air. } Optically they appear cleaner but in the TEM they are worse than before } they went into the plasma. The aperture ID edge appears to be populated } with hemispherical features that are prone to charging. } I considered back streaming from the fomblin filled mech. pump but } there is no residual smell of oil or sign of it anywhere about the } chamber. Does anyone have any idea what is going on & perhaps how these } apertures might be recovered? Some are cheap some are not. } } thanks, } Bruce Brinson } Rice U. Bruce, I've been cleaning apertures of all types for many years with 1 micron diamond paste and a cut-knap polishing cloth, although any lint-free cloth will work if the apertures are flat and fairly thin. Heavily counter-sunk apertures like Siemens 2mm really benefit from the cut-knap cloths (metallography supplies).
Finish by cleaning ultrsonically in Joy and hot water, blow dry immediatly.
As to what is going on in your plasma system, I can't help you there.
Ken Converse Quality Images third party SEM service Delta, PA
thanks for all your help in diagnosing my 35 problem. turns that the aperture heater was the culprit. i'll make note of this for "next time"!
b- ---------------------------------------------- Brian McIntyre Electron Microscopy Lab- River Campus Univ of Rochester Rochester, NY 14627 716-275-3058/4875
MSA Listers: Is John Russ offering training course on image analysis this year? When? Where? TIA.
Joseph Fu National Institute of Standards & Technology 100 Bureau drive Stop 8212 Gaithersburg, MD. 20899-8212 Tel: 301-975-3495 Fax: 301-869-0822 Email: jofu-at-nist.gov
I've had some experience with water immersion lenses. Their main advantage is higher numerical aperture than dry lenses at equivalent magnification, so better theoretical resolution. They don't offer quite as good resolution as oil immersion lenses, but they tend to have longer working distances, I believe, and don't have the messy clean-up of oil immersion lenses. So, yes, they do have some distinct advantages.
However, as others have pointed out, you may already be able to see everything you need to see with your current lenses. And nothing beats a test-drive. -- Gary P. Radice gradice-at-richmond.edu Associate Professor of Biology 804 289 8107 (voice) University of Richmond 804 289 8233 (FAX) Richmond VA 23173 http://www.science.richmond.edu/~radice
In a message dated 10/13/00 9:01:02 AM, jofu-at-nist.gov writes:
} Is John Russ offering training course on image analysis this year? When? } Where? TIA.
Thanks for the chance to plug the courses. There will be three sessions in 2001. Dates are May 9-11 and October 30-Nov. 1, in Raleigh (at N. C. State Univ) and June 6-8 in Denmark (at the Danish Technological Institute). Full info on the course contents, registration info, etc., (and a downloadable copy of the brochure for the 2000 courses, which is correct except that it does not have the year 2001 dates) is available on-line at http://members.aol.com/IPCourse/
It is also possible (and cost effective for groups of more than about 6-7 people) to arrange for an on-site course. Contact Cindy Allen in the NCSU Continuing Education department at 919 515 8171 for details.
I don't think there is an "optimum" thickness for the concrete slab. If vibration from the railroad (or any other source) is a concern most isolate the main slab by sawing & removing the concrete under the optics console. A new concrete slab is laid with "pea sized agregate" & felt on the sides. This lets the column stand free from the rest of the slab.
Regards,
Earl Weltmer
"Buckingham, Steve" wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi All, } I have a JSM 840 that I need to relocate soon. The new location is } very near a railroad and interstate! Does anyone have information on the } optimum thickness of a concrete slab to prevent vibrations? } Thanks, } } Steve Buckingham } Excellatron Solid State LLC } 1460 Roswell Street, Suite J } Smyrna, GA 30080 } phone 770 438 2201 } fax 770 438 2118
Many years ago we regularly used platinum crucibles in many analytical chemistry procedures. According to my analytical chemistry book, platinum can be cleaned by fusion with potassium or sodium bisulfite. I later used this procedure with good success to clean the platinum apertures for an RCA EML electron microscope. Get a small ceramic crucible, put a bit of the bisulfite in it, heat it until the bisulfite melts, then drop the apertures into the melt. After a few minutes allow the melt to solidify, dissolve the bisulfite with hot water, and your apertures should be as bright and shiny as new.
If this doesnn't work, try heating them in a mixture of equal parts of concentrated hydrofluoric acid and concentrated hydrochloric acid - using appropriate safety precautions, of course!
Good luck
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
To whom it may concern, I am a materials engineering student at the Univ of Cincinnati (Ohio) currently co-oping at a local aerospace company. They're wanting some microprobe analysis done on some parts and have put me in charge of that. I have a descent understanding of X-Ray diffraction but would like some information on the specifics of a microprobe so that I know exactly what is going on when I'm sitting watching the analysis take place. Is there any electronic information you can send or any sites you can refer me to that will give in depth information on the workings of a microprobe? Please respond to keithj-at-meyertool.com.
Based on your information we believe there may be burrs on the ID edge of your holes. We could open up the holes a bit to provide burr-free apertures, or we would allow you a trade in since as the only US Electron Microscope aperture manufacturer they probably originated with us anyway. If an aperture has a burr-free edge, a flamer should do for cleaning Pt and if they are Moly you can clean them in an evaporator. Even if your aperture is imported we would allow a trade in on a new disc or strip. Let me know if I can be of further help,
John Arnott --
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
web site http://www.laddresearch.com
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail sales-at-laddresearch.com
Quality Since 1955
Bruce Brinson wrote: }
} Hi E-beam buddies, } After our last excimer laser was shut down, I had the idea of } cleaning our Pt apertures with our plasma etcher using dry air. } Optically they appear cleaner but in the TEM they are worse than before } they went into the plasma. The aperture ID edge appears to be populated } with hemispherical features that are prone to charging. } I considered back streaming from the fomblin filled mech. pump but } there is no residual smell of oil or sign of it anywhere about the } chamber. Does anyone have any idea what is going on & perhaps how these } apertures might be recovered? Some are cheap some are not. } } thanks, } Bruce Brinson } Rice U.
Any feedback on the SEM images of the needles? Should I go ahead and finish the other 4 that way?
At only 25X mag, seems like LM would give better images, in color, for example, and the steel surfaces seem to show up better. The SEM images look a bit gray, as no metal coating and low kV imaging. Metal coating would show them up better, but I suppose that would not be allowed under the experimental constraints.
What do you think?
Gib
Gib Ahlstrand Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu http://biosci.umn.edu/MIC/consortium.html
Hi Bruce, } } I have never tried the plasma etcher but I suspect it would remove organics } effectively, just as a flame does. After several cleanings in the flame, we } are usually left with deposits similar to what you describe. My old Philips } 300 manual suggests these are usually silica-- this makes sense for our } samples, maybe for yours as well? Same manual advises "24 hrs. immersion in } 10% solution of HF in water". I usually save the ones I can't clean until I } have several, then turn them over to a colleague who works with HF regularly.
} Most come back as clean as new. The hemispherical features suggest to me that } our contaminant will melt, but not vaporize in the flame, consistent with } silica. You would have to look into the power of your etcher. Naturally, } these techniques are for solid Pt apertures, and HF is not for the } accident-prone. Good luck! By the way, our much newer Philips manual (XL30 } ESEM-FEG) gives 4 methods of heating but no mention of HF. } } Matt }
Matthew J. Lynn, Ph.D. Center for Advanced Microscopy University of Miami (305)284-4736 mlynn-at-miami.edu
The optimum thickness of concrete is whatever thickness of concrete it takes to stop the train. Any other answer is incorrect.
David C
} } ----- Original Message ----- } From: Earl Weltmer {earlw-at-pacbell.net} } To: Buckingham, Steve {sbuckingham-at-excellatron.com} } Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com} } Sent: Friday, October 13, 2000 3:49 PM } Subject: Re: Concrete slab for SEM } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hi Steve, } } } } I don't think there is an "optimum" thickness for the concrete slab. } } If vibration from the railroad (or any other source) is a concern most } isolate } } the main slab by sawing & removing the concrete under the optics console. } A } } new concrete slab is laid with "pea sized agregate" & felt on the sides. } This } } lets the column stand free from the rest of the slab. } } } } Regards, } } } } Earl Weltmer } } } } "Buckingham, Steve" wrote: } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } Hi All, } } } I have a JSM 840 that I need to relocate soon. The new location } is } } } very near a railroad and interstate! Does anyone have information on the } } } optimum thickness of a concrete slab to prevent vibrations? } } } Thanks, } } } } } } Steve Buckingham } } } Excellatron Solid State LLC } } } 1460 Roswell Street, Suite J } } } Smyrna, GA 30080 } } } phone 770 438 2201 } } } fax 770 438 2118 } } } } } } }
The optimum thickness of concrete is whatever thickness of concrete it takes to stop the train. Any other answer is incorrect.
David C
} } ----- Original Message ----- } From: Earl Weltmer {earlw-at-pacbell.net} } To: Buckingham, Steve {sbuckingham-at-excellatron.com} } Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com} } Sent: Friday, October 13, 2000 3:49 PM } Subject: Re: Concrete slab for SEM } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hi Steve, } } } } I don't think there is an "optimum" thickness for the concrete slab. } } If vibration from the railroad (or any other source) is a concern most } isolate } } the main slab by sawing & removing the concrete under the optics console. } A } } new concrete slab is laid with "pea sized agregate" & felt on the sides. } This } } lets the column stand free from the rest of the slab. } } } } Regards, } } } } Earl Weltmer } } } } "Buckingham, Steve" wrote: } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } Hi All, } } } I have a JSM 840 that I need to relocate soon. The new location } is } } } very near a railroad and interstate! Does anyone have information on the } } } optimum thickness of a concrete slab to prevent vibrations? } } } Thanks, } } } } } } Steve Buckingham } } } Excellatron Solid State LLC } } } 1460 Roswell Street, Suite J } } } Smyrna, GA 30080 } } } phone 770 438 2201 } } } fax 770 438 2118 } } } } } } }
It is not the thickness, but rather the area of the slab. It is better be minimal. Also, the soil under the slab- rock is bad, sand is good. If you have any choice on the matter- construction is not started yet, etc.- than have the slab on the sand cushion, make it small, just the size of the SEM footprint, and separate from the rest of the building. It is possible, in some cases, to cut the existing concrete slab in order to separate it from the rest of the structure. (Consult the construction expert first!!) Then, fill the cuts with soft silicone, rubber, etc. in order to prevent hard objects from getting in the cuts, as they will make an acoustic contact, which you are trying to avoid.
Also, many options from many sources are available regarding antivibration supports and mounts, depending on your situation and requirements. Some are expensive, some are not. Contact me off list if you need further help.
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax
This message is made of 100% recycled electrons. -----Original Message----- } From: Buckingham, Steve {sbuckingham-at-excellatron.com} To: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
Have you considered the speed, size, & direction of the train? In addition, the type of concrete & it's reinforcement material (ribar) also needs to be considered. Also whether you want the train to transmit vibration, collide with the SEM or land on top of the SEM needs to be calculated.
Any other answer is incorrect.
Regards,
Earl
David Cockayne wrote:
} Earl } } The optimum thickness of concrete is whatever thickness of concrete it } takes to stop the train. Any other answer is incorrect. } } David C } } } } } ----- Original Message ----- } } From: Earl Weltmer {earlw-at-pacbell.net} } } To: Buckingham, Steve {sbuckingham-at-excellatron.com} } } Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com} } } Sent: Friday, October 13, 2000 3:49 PM } } Subject: Re: Concrete slab for SEM } } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } Hi Steve, } } } } } } I don't think there is an "optimum" thickness for the concrete slab. } } } If vibration from the railroad (or any other source) is a concern most } } isolate } } } the main slab by sawing & removing the concrete under the optics } console. } } A } } } new concrete slab is laid with "pea sized agregate" & felt on the sides. } } This } } } lets the column stand free from the rest of the slab. } } } } } } Regards, } } } } } } Earl Weltmer } } } } } } "Buckingham, Steve" wrote: } } } } } } } } ------------------------------------------------------------------------ } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } -----------------------------------------------------------------------. } } } } } } } } Hi All, } } } } I have a JSM 840 that I need to relocate soon. The new } location } } is } } } } very near a railroad and interstate! Does anyone have information on } the } } } } optimum thickness of a concrete slab to prevent vibrations? } } } } Thanks, } } } } } } } } Steve Buckingham } } } } Excellatron Solid State LLC } } } } 1460 Roswell Street, Suite J } } } } Smyrna, GA 30080 } } } } phone 770 438 2201 } } } } fax 770 438 2118 } } } } } } } } } } }
NYSEM Presidential Symposium 2000: "Cell Polarization: Mechanisms at the Membrane"
The New York Society for Experimental Microscopy will hold its annual Presidential Symposium on 19 October from 9:00 AM to 5:30 PM at Columbia University (Hammer Health Sciences Building, Room 401*). This year's Symposium is entitled: "Cell Polarization: Mechanisms at the Membrane". The Symposium will focus on recent breakthroughs in understanding the molecular mechanisms involved in the generation of cell polarity. Symposium participants will highlight recent advances in a number of model systems in which microscopic approaches can be combined with genetic and molecular biological approaches to understand cell polarity. One emerging theme that will be explored during the Symposium is the degree to which basic molecular pathways for cell polarization have been conserved despite the diversity of structures that result in the different systems. Registration is free, but preregistration is required for lunch (contact Dr. Philip Leopold, Cornell Univ. Medical School, email: pleopold-at-mail.med.cornell.edu; phone 212-746-2255)
* The Hammer Health Sciences Building is one block west of Broadway on the corner of Fort Washington and 168th Street. There is a subway stop at Broadway and 168th Street.
SCHEDULE - NYSEM PRESIDENTIAL SYMPOSIUM 2000 "Cell Polarization: Mechanism at the Membrane" 19 October 2000 Columbia University Hammer Health Sciences Building, Room 401
9:15 - 9:55 John Cooper (Department of Cell Biology & Physiology, Washington University, St. Louis): "Interactions of Microtubules with the Cortex During Mitosis in Budding Yeast"
9:55 - 10:35 Kerry Bloom (Department of Biology, University of North Carolina - Chapel Hill "How Microtubules Polarize Nuclear Movements in Yeast"
10:35 - 10:55 Coffee Break
10:55 - 11:35 Fred Chang (Department of Microbiology, Columbia University, New York) "Spatial control and cytoskeletal dynamics in fission yeast"
11:35 - 12:15 Carole Parent (National Cancer Institute, NIH, Bethesda) "Visualizing Signaling Events in Chemotaxing Eukaryotic Cells"
2:00 - 2:40 Gregg Gundersen (Department of Anatomy & Cell Biology, Columbia University, New York) "Rho GTPase Pathways Integrate the Microtubule and Actin Cytoskeletons During Cell Migration"
2:40 - 3:20 Clare Waterman-Storer (Department of Cell Biology, Scripps Research Institute, La Jolla) "Microtubule and Actin Interactions in Cell Polarization and Motility"
3:20 - 3:40 Coffee Break
3:40 - 4:20 Enrique Rodriguez-Boulan (Dyson Vision Research Institute, Cornell University, New York) "Role of Actin Filaments and Microtubules in Polarized Delivery of Plasma Membrane Proteins"
4:20 - 4:30 Gregg Gundersen - Closing Remarks
4:30 - 5:30 Reception - River View Lounge
**************************************************************************** Frank Macaluso tel: 718-430-3547 Analytical Imaging Facility fax: 718-430-8996 Albert Einstein College of Medicine e-mail: macaluso-at-aecom.yu.edu 1300 Morris Park Avenue http://www.aecom.yu.edu/aif/ Bronx, NY 10461 ****************************************************************************
---------------------- Forwarded by Paula M Churt/TMG/CSC on 10/13/2000 10:59 AM ---------------------------
Diane A Ciaburri/GDDS-at-GDDS 10/13/2000 09:55 AM
To: Paula M Churt/TMG/CSC-at-CSC cc:
Hello
Has anyone observed biological material-antibody-dynabead complex in EM? Our user has great results with Dynabeads M-450 Epoxy Product # 140.01 in his assay, but we would like to visualize the virus attached to the dynabead. The manufacturer suggests the iron core may leak on sectioning causing contamination. Any ideas? Thanks
Theresa
Theresa A. Fassel Core EM Unit, Mail Box MB-32 The Scripps Research Institute 10,550 N. Torrey Pines Rd. LaJolla, CA 92037-1027 tel: (858)-784-8182 fax: (858)-784-8193 tfassel-at-scripps.edu
The optimum thickness of concrete is whatever thickness of concrete it takes to stop the train. Any other answer is incorrect.
David C
----- Original Message ----- } From: Earl Weltmer {earlw-at-pacbell.net} To: Buckingham, Steve {sbuckingham-at-excellatron.com} Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com} Sent: Friday, October 13, 2000 3:49 PM
I run in the ETCH mode with Argon. Set power level to 5%. Put in the dummy Aluminum target. Hit PROCESS and keep the vacuum at 60mTorr-40mTorr. Try for about 30 seconds and then look at the standard. Repeat until clean. The lower the vacuum level, the less aggressive is the cleaning action. The plasma will strike when the vacuum reaches 20mTorr in the ETCH mode; 40mTorr in the PLATE mode. When done, remove the Al target and put back in the sputter coater target.
I coat (PLATE) at 80mTorr, 5nM/minute. This gives a nice, smooth and even coating with Au/Pd or Pt.
I'm using a Hummer VII. BTW, if you have trouble with the plasma not striking for PLATE when vacuum reaches 40mTorr, I have a simple fix that makes it virtually never fail to fire.
gary g.
At 07:59 AM 10/13/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } 10/13/00 Subject: Cleaning of SEM Mag } Standard } 09:36 } AM } } } } } } } } } Hi all, } } I've got an NIST 484e SEM Mag Standard that has years of carbon build-up } ("burn marks"). I want to clean it with my plasma etcher but I'm not sure } about the parameters to use. Does anyone have experience doing this? } } I have an Anatech Hummer plasma coater. Do I use the etch or plasma mode? } Argon or air for atmosphere? What vacuum and voltage, and for how long? } } Thanks! } } Diane Ciaburri } } dciaburri-at-gdds.com } (413)494-2847 } General Dynamics } 100 Plastics Ave. } Pittsfield Ma 01201
David is right. Our testing has shown that saw cutting the floor to isolate the column from the rest of the building has no affect other than to cost a lot. The vibration is transmitted through the ground, not the bldg.
Craig Franklin Vibration Engineering Consultants www.vibeng.com
----- Original Message ----- } From: David Cockayne {david.cockayne-at-materials.oxford.ac.uk} To: {Microscopy-at-sparc5.microscopy.com} Sent: Friday, October 13, 2000 5:16 PM
I don't know that the beads "leak", but they do not present a smooth appearance in sections, either internally or - sometimes - at the surface. the internal appearance can be discounted, but I always process some control ("clean") beads in parallel. Sometimes there is a little fluffy stuff (OK - flocculent material) on the surface of the blank beads, but it could never (in my experience) be confused with viruses.
One other thing I would suggest is to have your researcher try to get as much material aspossible on small beads. I once examined some IP samples that were not very efficiently done on large beads - between the large surface area of the beads and the low density of actual sample on the beads, a *lot* of time was wasted just looking for the IP material. A real aspirin project :)
I section Dynabeads on the not-so-great part of my diamond knife; I haven't noticed new scratches from it, but I suspect that the material is potentially dulling and scratchy.
Good luck!
Tamara Howard CSHL
On Fri, 13 Oct 2000, Theresa Fassel wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello } } Has anyone observed biological material-antibody-dynabead complex in EM? } Our user has great results with Dynabeads M-450 Epoxy Product # 140.01 in } his assay, but we would like to visualize the virus attached to the } dynabead. The manufacturer suggests the iron core may leak on sectioning } causing contamination. Any ideas? } Thanks } } Theresa } } } Theresa A. Fassel } Core EM Unit, Mail Box MB-32 } The Scripps Research Institute } 10,550 N. Torrey Pines Rd. } LaJolla, CA 92037-1027 } tel: (858)-784-8182 } fax: (858)-784-8193 } tfassel-at-scripps.edu } }
Hi Ruth; I would highly recommend the Ted Pella Microwave oven series 3450. I have used this oven for over 3 years now and have processed over 500 specimens (human and animal) without a single mishap to the tissue. In fact you may find the results better then you would get by conventional processing. You can contact Rick Giberson with Ted Pella's toll free number and he can give you more specific information and a price to work with. You can contact me at rla-at-mindspring.com as well.
Ron Austin (Research Associate) LSU Medical Ct. at Shreveport Dept. of Pathology Shreveport, LA 318-675-4557
The following is a request from a producer of the PBS TV show NOVA, which came in to the MSA Business office.
Can anyone help? Please correspond directly to the requester.
} } } } } }
Hi Folks --
I'm a producer with the science TV series NOVA. I'm working on a show about evolution and I'm desperately looking for a way to adapt a zeiss axiophot microscope to an Aaton 16mm film camera. So far I've struck out on finding anyone who has gear to accomplish this feat.
Any suggestiongs?
-- Chris Schmidt Powderhouse Productions 259 Elm Street Somerville MA 02144 617-629-2200 work 617-776-7905 fax 781-820-0727 mobile 617-403-8699 pager 781-646-0928 home Chris-at-powderhouse.net
Increasing the mass under the microscope works. An isolated room-size slab of 1m thickness can reduce vertical vibration by a factor of four and horizontal vibration by 10 to 12 times [1]. This foundation was a significant factor in allowing us the obtain a TEM resolution better than one Angstrom [2].
-Mike O'Keefe
1. "Design and implementation of a site for a one-Ångstrom TEM", John H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
----- Original Message ----- } From: "Craig Franklin" {franklin-at-vibeng.com}
Craig, David, Earl, Steve,
Increasing the mass under the microscope works. An isolated room-size slab of 1m thickness can reduce vertical vibration by a factor of four and horizontal vibration by 10 to 12 times [1]. This foundation was a significant factor in allowing us the obtain a TEM resolution better than one Angstrom [2].
-Mike O'Keefe
1. "Design and implementation of a site for a one-Ångstrom TEM", John H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
----- Original Message ----- } From: "Craig Franklin" {franklin-at-vibeng.com}
Craig, David, Earl, Steve,
Increasing the mass under the microscope works. An isolated room-size slab of 1m thickness can reduce vertical vibration by a factor of four and horizontal vibration by 10 to 12 times [1]. This foundation was a significant factor in allowing us the obtain a TEM resolution of better than one Angstrom [2].
-Mike O'Keefe
1. "Design and implementation of a site for a one-Ångstrom TEM", John H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
----- Original Message ----- } From: "Craig Franklin" {franklin-at-vibeng.com}
Craig, David, Earl, Steve,
Increasing the mass under the microscope works. An isolated room-size slab of 1m thickness can reduce vertical vibration by a factor of four and horizontal vibration by 10 to 12 times [1]. Such a foundation was a significant factor in allowing us to obtain a TEM resolution better than one Angstrom [2].
-Mike O'Keefe
1. "Design and implementation of a site for a one-Ångstrom TEM", John H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
----- Original Message ----- } From: "Craig Franklin" {franklin-at-vibeng.com}
Craig, David, Earl, Steve,
Increasing the mass under the microscope works. An isolated room-size slab of 1m thickness can reduce vertical vibration by a factor of four and horizontal vibration by 10 to 12 times [1]. Such a foundation was a significant factor in allowing us to obtain a TEM resolution better than one Angstrom [2].
-Mike O'Keefe
1. "Design and implementation of a site for a one-Ångstrom TEM", John H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
----- Original Message ----- } From: "Craig Franklin" {franklin-at-vibeng.com}
} I have a descent understanding of X-Ray diffraction but would like some } information on the specifics of a microprobe so that I know exactly what is } going on when I'm sitting watching the analysis take place. Is there any } electronic information you can send or any sites you can refer me to that } will give in depth information on the workings of a microprobe?
Keith
There is a wonderful book "Handbook of Silicate Analysis", by P J Potts, that has great chapters on many modern instrumental techniques, including XRF and EPMA. Although written with a geological slant, Potts combines lucidity with solid info, and it should be interesting to you also.
cheers
rtch
ps I hope your XRD is located downstairs.
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Thanks for the heads-up. I was working from home this morning (Sunday) and finally noticed and stopped it. I hoped it had only gone out once or twice, but I had received five copies this afternoon when I checked the listserver. :-(
Cheers, Mike
----- Original Message ----- } From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
To Mike, Craig, Earl and others,
Many years ago there was an article in JEOL News detailing the design of floors for electron microscopes at the John Innes Institute in the UK, and more recently I saw similarly detailed plans for a new facility in Australia (in Brisbane, I think). We have incorporated here aspects of the John Innes Institute plans, and do not have a vibration problem, but as we do not have trains that run anywhere nearby, unfortunately I have no idea whether or not these floors would be effective in reducing the vibration being discussed!
Rob
On 15 Oct 00, at 19:03, MAOKeefe-at-lbl.gov wrote:
} ---------------------------------------------------------------------- } -- The Microscopy ListServer -- Sponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } Craig, David, Earl, Steve, } } Increasing the mass under the microscope works. An isolated room-size } slab of 1m thickness can reduce vertical vibration by a factor of four } and horizontal vibration by 10 to 12 times [1]. This foundation was a } significant factor in allowing us the obtain a TEM resolution better } than one Angstrom [2]. } } -Mike O'Keefe } } 1. "Design and implementation of a site for a one-Ångstrom TEM", John } H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. } MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom } Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and } Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000). } } ----- Original Message ----- } } From: "Craig Franklin" {franklin-at-vibeng.com} } Date: Saturday, October 14, 2000 8:11 am } Subject: Re: Concrete slab for SEM } } } ------------------------------------------------------------------- } } ----- The Microscopy ListServer -- Sponsor: The Microscopy Society } } of America To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.ComOn-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html-------- } } ---------------------------------------------------------------. } } } } } } Steve, } } } } David is right. } } Our testing has shown that saw cutting the floor to isolate the } } column from the rest of the building has no affect other than to } } cost a lot. The vibration is transmitted through the ground, not the } } bldg. } } } } Craig Franklin } } Vibration Engineering Consultants } } www.vibeng.com } } } } } } ----- Original Message ----- } } } From: David Cockayne {david.cockayne-at-materials.oxford.ac.uk} } } To: {Microscopy-at-sparc5.microscopy.com} } } Sent: Friday, October 13, 2000 5:16 PM } } Subject: Re: Concrete slab for SEM } } } } } } } ----------------------------------------------------------------- } } ------- } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } America} To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com} On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} ------ } } -----------------------------------------------------------------. } } } } } } } } } Earl } } } } } } The optimum thickness of concrete is whatever thickness of } } concrete it } } } takes to stop the train. Any other answer is incorrect. } } } } } } } } } David C } } } } } } } } } ----- Original Message ----- } } } } From: Earl Weltmer {earlw-at-pacbell.net} } } } To: Buckingham, Steve {sbuckingham-at-excellatron.com} } } } Cc: 'Microscopy-at-MSA.Microscopy.Com' } } {Microscopy-at-sparc5.microscopy.com} } Sent: Friday, October 13, 2000 } } 3:49 PM } } } Subject: Re: Concrete slab for SEM } } } } } } } } } } --------------------------------------------------------------- } } --------- } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society } } of America } } } } To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com} } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } ---- } } -------------------------------------------------------------------. } } } } } } } } } } } } Hi Steve, } } } } } } } } I don't think there is an "optimum" thickness for the concrete } } slab.} } If vibration from the railroad (or any other source) is a } } concern most } } } isolate } } } } the main slab by sawing & removing the concrete under the optics } } console. } } } A } } } } new concrete slab is laid with "pea sized agregate" & felt on } } the sides. } } } This } } } } lets the column stand free from the rest of the slab. } } } } } } } } Regards, } } } } } } } } Earl Weltmer } } } } } } } } "Buckingham, Steve" wrote: } } } } } } } } } } } ----------------------------------------------------------------- } } ------- } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society } } } } } of } } America } } } } } To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com } } } } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } ----------------------------------------------------------------- } } ------. } } } } } } } } } } Hi All, } } } } } I have a JSM 840 that I need to relocate soon. The new } } location } } } is } } } } } very near a railroad and interstate! Does anyone have } } information on } } the } } } } } optimum thickness of a concrete slab to prevent vibrations? } } } } } Thanks, } } } } } } } } } } Steve Buckingham } } } } } Excellatron Solid State LLC } } } } } 1460 Roswell Street, Suite J } } } } } Smyrna, GA 30080 } } } } } phone 770 438 2201 } } } } } fax 770 438 2118 } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa **
From root Mon Oct 16 02:48:57 2000 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id CAA19424 for dist-Microscopy; Mon, 16 Oct 2000 02:31:45 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id CAA19421 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Mon, 16 Oct 2000 02:31:14 -0500 (CDT) Received: from oxmail.ox.ac.uk (oxmail2.ox.ac.uk [163.1.2.1]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id CAA19414 for {Microscopy-at-sparc5.microscopy.com} ; Mon, 16 Oct 2000 02:31:03 -0500 (CDT) Received: from ermine.ox.ac.uk ([163.1.2.13]) by oxmail.ox.ac.uk with esmtp (Exim 3.12 #1) id 13l4j0-0003Lg-00 for Microscopy-at-sparc5.microscopy.com; Mon, 16 Oct 2000 08:30:22 +0100 Received: from cockayne.materials.ox.ac.uk ([163.1.65.199] helo=cockayne) by ermine.ox.ac.uk with smtp (Exim 3.13 #1) id 13l4j0-00073J-00 for Microscopy-at-sparc5.microscopy.com; Mon, 16 Oct 2000 08:30:22 +0100 Message-ID: {001701c03742$383d8920$c74101a3-at-materials.ox.ac.uk}
Dear Mike
I am sure that increasing the mass under the microscope works, as you say. But the question was what is the "optimum thickness to stop vibrations". There is only one "optimum", and I believe my answer was it.
David ----- Original Message ----- } From: {"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com} To: Craig Franklin {franklin-at-vibeng.com} Cc: MSA List Server {Microscopy-at-sparc5.microscopy.com} ; DavidCockayne {david.cockayne-at-materials.oxford.ac.uk} Sent: Sunday, October 15, 2000 8:03 PM
Craig, David, Earl, Steve,
Increasing the mass under the microscope works. An isolated room-size slab of 1m thickness can reduce vertical vibration by a factor of four and horizontal vibration by 10 to 12 times [1]. This foundation was a significant factor in allowing us the obtain a TEM resolution better than one Angstrom [2].
-Mike O'Keefe
1. "Design and implementation of a site for a one-Ångstrom TEM", John H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
----- Original Message ----- } From: "Craig Franklin" {franklin-at-vibeng.com}
Colleagues...
Can anyone recommend a introductory text for this person. Remember Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS looking for help so keep try to your technical details at the right level.
Below is the result of your feedback form. It was submitted by (sfield-at-island.net) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday, October 15, 2000 at 23:18:56 ---------------------------------------------------------------------------
Email: sfield-at-island.net Name: Shane Field
School: Hard Knocks
State: BC
Zip: V0N 3E0
Question: I recently came into possession of an old Bushnell microscope (the kind that is kept in some high school science labs for 2 or 3 generations). On the top of the scope it says--CENCO 506-L Bushnell Triple Tested 667157. (This may have been the 57 Chev 283 of scopes found in biology classrooms all over North America) Anyway, I took Physics and Chemistry in high school and never did learn how to operate a simple microscope. Imagine my chagrin at not being able to figure out how it works --I am able to view objects at low power but not high power. Would you be able to reccomend a good (but simple) Coles Notes kind of booklet that will give me the basics of lighting,focussing/eye piece selection etc? etc.? Thank you-TY TY TY!
At 4:03 PM -0500 10/13/00, Theresa Fassel wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Greetings, Years ago, I made 16 mm movies from a microscope as follows. Hooked up an ordinary video camera to the scope and displayed the image on a high quality monitor. In front of the monitor was a wooden square box that telescoped and was black on the inside. At the end of that was a 16 mm movie camera, focussed on the monitor. One frame of the move was exposed in a 1/4 sec exposure so we were running much slower than the tv raster rate. We were actually doing timelapse studies like this and got very nice images for playback and analysis. This was done in the lab of Ray Keller at UC Berkeley who would have much more of the details to hand if you want to follow this route up.
Could this help?? Tobias Baskin
} The following is a request from a producer of the PBS TV show NOVA, which } came in to the MSA Business office. } } Can anyone help? Please correspond directly to the requester. } } } } } } } } } } Hi Folks -- } } I'm a producer with the science TV series NOVA. I'm working on a show } about evolution and I'm desperately looking for a way to adapt a zeiss } axiophot microscope to an Aaton 16mm film camera. So far I've struck } out on finding anyone who has gear to accomplish this feat. } } Any suggestiongs? } } -- } Chris Schmidt } Powderhouse Productions } 259 Elm Street } Somerville MA 02144 } 617-629-2200 work } 617-776-7905 fax } 781-820-0727 mobile } 617-403-8699 pager } 781-646-0928 home } Chris-at-powderhouse.net
Another poster related his success at using an intermediate monitor for getting the image onto 16 mm film. I might take that a step further.
I don't know film production techniques, but was of the impression that much of the editing has gone digital. If that be the case, are there not utilities to record high resolution digital movies from microscope cameras. I thought Sony had a rather decent digital video camera that John McKenzie was touting a year or two ago. Even better cameras should be available now.
Please remember the final use and display of the sequence. I know that film can provide greater detail than digital for still photographs, but I doubt that this sequence would be enlarged to the degree that resolution would be an issue. Then there is the question of final display. If I viewed the final result on my plain, old TV, most any camera system would provide more resolution than I could appreciate. If it was viewed on an HDTV, more detail would be visible, but still not as much as a good video camera should be able to provide. Maybe an IMAX presentation would require all the resolution a camera could provide and then some. But by then, I would guess the resolution of the microscope would be more of a limitation than the resolution of the camera.
In short, I would suggest collecting and processing the sequence in digital format until the final stage and then transferring it to 16-mm if need be.
Warren Straszheim
At 01:04 PM 10/15/2000 -0400, you wrote:
} The following is a request from a producer of the PBS TV show NOVA, which } came in to the MSA Business office. } } Can anyone help? Please correspond directly to the requester. } } } } } } } } Hi Folks -- } } I'm a producer with the science TV series NOVA. I'm working on a show } about evolution and I'm desperately looking for a way to adapt a zeiss } axiophot microscope to an Aaton 16mm film camera. So far I've struck } out on finding anyone who has gear to accomplish this feat. } } Any suggestiongs? } } -- } Chris Schmidt } Powderhouse Productions } 259 Elm Street } Somerville MA 02144 } 617-629-2200 work } 617-776-7905 fax } 781-820-0727 mobile } 617-403-8699 pager } 781-646-0928 home
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
After attempting a test roll of Polapan CT 35mm instant film, I discovered what "outdated" means in the Polaroid context - the negative failed to strip off the celluloid and the strip looked unusable.
Later, after finding that there are no known dealers of this film in my area (SE PA) I retrieved the film from the waste basket & washed it in warn water, whereupon, to my utter amazement, usable positive images appeared out of the murk.
I am now waiting for the film strip to dry, after dipping it in PhotoFlo as I am used to doing with Type 55 P/N negatives.
Is there a more conservative way to strip the negative off the film ? My method seems rather crude ...
Best regards, George Langford amenex-at-amenex.com
} Craig, David, Earl, Steve, } } Increasing the mass under the microscope works. An isolated room-size } slab of 1m thickness can reduce vertical vibration by a factor of four } and horizontal vibration by 10 to 12 times [1]. This foundation was a } significant factor in allowing us the obtain a TEM resolution better } than one Angstrom [2]. } } -Mike O'Keefe } } 1. "Design and implementation of a site for a one-Ångstrom TEM", John } H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. } MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom } Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and } Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000). } The concrete slab need to sit on dry sand to minimize coupling to the soil/rock below it. A layer of gravel with drain tile laid in it covered by a 2 or 3 foot layer of sand would be a starting point. If the drain tile won't drain by gravity a sump will be needed to pump out any water. A water barrier need to be placed below the gravel. The truly paranoid would put down 6 inches of tamped sand, 4 inches of benotnite clay, a layer of heavy plastic enough sand to keep the installation of gravel from puncturing the plastic. The benonite serves as a self sealing moisture barrier. It is a clay that swells when it gets wet. It is not particularly expensive if you buy it from a oil field mud company. They buy it in train car loads and sell it in truck loads or sacks.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
} Question: I recently came into possession of an old Bushnell microscope } (the kind that is kept in some high school science labs for 2 or 3 } generations). On the top of the scope it says--CENCO 506-L Bushnell Triple } Tested 667157. (This may have been the 57 Chev 283 of scopes found in } biology classrooms all over North America) Anyway, I took Physics and } Chemistry in high school and never did learn how to operate a simple } microscope. Imagine my chagrin at not being able to figure out how it works } --I am able to view objects at low power but not high power. Would you be } able to reccomend a good (but simple) Coles Notes kind of booklet that will } give me the basics of lighting,focussing/eye piece selection etc? etc.? } Thank you-TY TY TY! } Shane -
I hope that you'll be willing to go a step beyond "booklet". There's a very good basic book; here's the review from the Project MICRO bibliography (URL below):
Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound. 6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY. Although this book is intended for adult amateur microscopists, it is well written and will provide teachers and classroom volunteers with much useful information on "serious" light microscopy; it's unequalled as a basic reference for beginners. Almost half of the book is devoted to simple preparation methods for biological specimens and descriptions (with good illustrations) of commonly encountered organisms. Adult. RECOMMENDED
If that's beyond your butget, shop around the MICRO bibliography. You may find enough basic info in the website hotlink list.
Caroline
Caroline
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
Hi George, Well, George if you have talked to Rick and have been to a workshop I don't know if I have any solutions for you, however, you said you were mostly processing yeast and Drosphila Fly eyes. In my earlier day in EM I work for a very short time on plant tissue and I found that I had to use a vacuum device of some kind to get the resin to penetrate the tissue. Pella has a Vacuum device you can use in the model of oven you have (which by the way is the same as mine). I found that nerve tissue take a little bit more fixation (3% Glut. in 0.1M Cacodylate buffer pH 7.3) in the oven then other tissue does. The nerve I mostly work with is sural nerve and I find that a temp setting of 37C with an irritation time of 40sec x3. Cooling the tissue down, in wet ice, to about 7-8C before and between each irritation period works for me. What sort of fixative do you use with these tissues?
I have a vacuum system for that microwave here at Shreveport which is only a 3 or 4 hour trip from Dallas. Maybe you could work sometime in to come over, bring your specimens with you.
Ron Austin (Research Associate) LSU Medical Ct. Dept. of Pathology 318-675-4557 Shreveport, LA e-mail: rla-at-mindspring.com
Could you please help us - please send replies to my email address:
c.hayward-at-kingston.ac.uk
Hi, We have a 30 year old Philips T301 transmission electron microscope, and the rubber roll membrane (original part number 5322 360 40071) in the pneumatic film lifting mechanism of the camera has split. In view of the age of the instrument it looks as if we would only be able to replace it if some nice person out there has a spare one that they might be prepared to part company with. Is there anybody out there who has a spare camera lift roll membrane please?
Thanks,
Bill Edwards.
Dr. C. Hayward-Costa School of Life Sciences Kingston University Penrhyn Road, Kingston upon Thames Surrey KT1 2EE, UK 44(0)208 547 2000 x 2240 Email: c.hayward-at-kingston.ac.uk
The principle of mounting the microscope on a massive slab is good, but I suspect that to isolate your microscope from the very low frequency, long-wavelength vibrations caused by rail traffic you will need a much softer and compliant isolation than can be provided by any depth of sand or compacted aggregate. I have recently built a garage / workshop within 50 metres of the London - Edinburgh track. The building is on a heavy reinforced concrete raft foundation. The slab is on 12" of sand over 3 feet of 2 to 3" aggregate and the substrate is several metres of heavy gravel material described by the geologists as raised beach. Nevertheless you can feel the vibration caused by passing trains through your feet.
My guess is that you need a fully floating slab, either mounted on very compressible pads of rubber or similar elastomer, or raised on an air cushion.
Vibration is a problem which many people worry about as demands for high resolution and traffic increase, and solutions to it are expensive. It strikes me that it would be a good move to do some modelling of the physics of the situation before committing money to it. Does anyone know how to go about this? Does software exist?
Chris
} From: "Gordon Couger" {gcouger-at-couger.com} To: {R.Cross-at-ru.ac.za} , {"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com} Copies to: {Microscopy-at-sparc5.microscopy.com}
Food Structure & Functionality Symposium 2001 - An international symposium leading Food Structure & Functionality studies through the 21st century
Being held in conjunction with the 92nd AOCS Annual Meeting and Expo , May 13-16, 2001, Minneapolis Convention Center, Minneapolis, Minnesota, USA. For information, e-mail us at meetings-at-AOCS.org
The mandate of the Food Structure & Functionality Forum : *To promote global collaboration between Food and Agriculture professionals in Structure and Functionality disciplines by facilitating and providing a forum for exchange of knowledge, expertise and research findings*. The symposium has two themes: * new and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure-function relationships in foods; * food system studies covering any part of the processing chain - from the raw material to the final product, and including trouble shooting. preliminary program May 13th - Short Courses (each short course will take a full day, they will be run in parallel) 1) Food Contaminants. Contact person - Mark Auty 2) Specific Labelling in Foods. Contact person - Marcel Paques
May 15th-17th inclusive - Technical sessions (6 sessions will run over 3 days)
May 14th Morning. Session 1. Dairy applications and fat based foods (Chairs: Mark Auty and Tony McKenna)
Afternoon Session 2. Food Safety (Chairs: Judy Arnold and Maria Brandl )
Evening -Round Table Discussion (RTD) -Social program for Division members and FS&F symposium participants
May 15th Morning Session 3. New Methods and Techniques for Food Structure and Functionality Analysis Chair: Kathy Groves
Afternoon Session 4. Agricultural Applications of Microscopy and Imaging (Chairs: Delilah Wood and Paula Allan-Wojtas)
Evening FS&F Division member meeting
May 16th Morning Session 5. Ingredients and Food Processing (Chairs: Diana Kittleson and Jim Charbonneau)
Afternoon 6. Colloidal and Interfacial Sciences (Chairs: Marcel Paques and David Pechak)
Poster Session Posters will be displayed during the meeting and presented during breaks between technical sessions
Contact information for the chairs is shown below, in alphabetical order:
Paula Allan-Wojtas Agriculture and Agri-Food Canada Atlantic Food and Horticulture Research Centre Kentville, Noval Scotia Canada B4N 1J5 Tel: (902) 679-5566 FAX: (902) 679-2311 email: allanwojtasp-at-em.agr.ca
Judy Arnold USDA -ARS - RRC 950 College Station Rd. P.O. Box 5677 Athens, GA 30604-5677 USA Tel: (706) 546-3515 FAX: (706) 546-3068 email: jarnold-at-ars.usda.gov
Mark Auty Dairy Products Research Centre TEAGASC Moorepark, Femoy, Co. Cork Ireland Tel: 011-353-25-42447 FAX: 011-353-25-42340 email: mauty-at-moorepark.teagasc.ie
Maria Brandl USDA - ARS - WWRC 800 Buchanan Street Albany, CA 94710 USA Tel: (510) 559-5885 FAX: (510) 559-5948 email:mbrandl-at-pw.usda.gov
James E. Charbonneau National Food Processors Association Food Chemistry and Packaging Department 1401 New York Ave, NW Washington, D.C. 20005 USA Tel: (202) 639-5972 FAX: (202) 639-5991 email: jcharbo-at-nfpa-food.org
Kathy Groves Leatherhead Food Research Association Randalls Road, leatherhead Surrey KT22 7RY England Tel: 44 0132 822330 FAX: 44 0132 386228 email: kgroves-at-lfra.co.uk
Tony McKenna New Zealand Dairy Institute Private Bag 11 029 Palmerston North, New Zealand Tel: 011 64 6 350 4649 FAX: 011 64 6 356 1476 email: tony.mckenna-at-nzdri.org.nz
Marcel Paques Wangeningen Centre for Food Sciences/Unilever Research Vlaardingen P.O. Box 20, 6710 BA Ede The Netherlands Tel: 011 31 318 659690 FAX: 011-31-318 650400 email: paques-at-nizo.nl
David Pechak Kraft Technology Centre 801 Waukegan Road Glenview, IL 60025 USA Tel: (847) 646-4808 FAX: (847) 646-3864 email: dpechak-at-kraft.com
Delilah Wood USDA - ARS - WWRC 800 Buchanan Street Albany, CA 94710 USA Tel: (510) 559-5653 FAX: (510) 559-5777 email: wood-at-pw.usda.gov
All this talk of huge amounts of concrete and agregate and sand. . .
What about isolation platforms? While it might be slightly more expensive - at least you won't be left with a huge construction bill if your concrete slab doesn't isolate enough vibration.
TMC makes specific floor platforms. We were looking at their tables when ordering a Confocal Mircosope. And from the catalog and price list you can get a platform that will easily isolate the column from nearly all vibration for around $5000 to $6000 dollars. It looks like they also make active vibration cancelling systems too.
Simple things like elastomer and rubber bases for concrete slabs have a fatigue life, which could be shorter than the remain life of the microscope.
I have no finacial interest in TMC: http://www.techmfg.com
I cannot see how you will isolate train vibrations without a system a little more complex than a concrete slab.
Geoff Williams
Electron Microscope Facility Supervisor Biology Department Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
} } } "Buckingham, Steve" {sbuckingham-at-excellatron.com} 10/12/00 18:55 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi All, I have a JSM 840 that I need to relocate soon. The new location is very near a railroad and interstate! Does anyone have information on the optimum thickness of a concrete slab to prevent vibrations? Thanks,
Steve Buckingham Excellatron Solid State LLC 1460 Roswell Street, Suite J Smyrna, GA 30080 phone 770 438 2201 fax 770 438 2118
Steve,
Have you tried something cheaper? When I was with The Johns Hopkins University School of Medicine we were remodeling the lab. A new darkroom sink for developing negatives was being put in right beside the TEM. The plumbers had to drill for water lines and a sink drain within 3-5 feet of the microscope. We lifted up each corner of the microscope and installed 60 or 80 durometer(I think it was 80), 4"x4"x1" rubber pads and could use the microscope without vibration. The lab was located just below street level. This might be a way to go before trying concrete slabs. The only way I've heard of using concrete slabs was to isolate the slab from the rest of the floor. A concrete slab could be very expensive. Most rubber supply houses can supply the pads. Hope you can work it out.
Phil Rutledge USDA/ARS 151 Dixon Drive Chestertown, MD 21620 voice: 410.778.4136 or 2120 fax: 410.778.4399 e-mail: rutledge-at-ars.usda.gov
Videotapes from M&M 2000 are now available for purchase. A new list of all tapes in the collection can be found at the MSA web site or directly at : http://www.biotech.ufl.edu/sems/newtape00.htm
Many of the older tapes are available for immediate shipment. Others, including the 2000 tapes, must be ordered from the video duplication service. This usually requires about two weeks. Please remember to order the tapes by number and title. Make checks payable to "MSA" in US funds only. Purchase orders can be accepted. Orders may be placed by phone, mail, fax or e-mail to the contact at the bottom of this message.
If you are a presenter in any of the tapes and feel that your tape should not be offered for sale in the future for whatever reason (obsolete information, bad hair day, etc.) please let me know.
Greg Erdos Videotape Wrangler Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program PO Box 110580 University of Florida Gainesville, FL 32611
Can anyone answer this one?. Remember Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS and K-12 Students looking for help so keep try to your technical details at the right level.
Nestor --------------------------------------------------- Below is the result of your feedback form. It was submitted by (crowj-at-mail.leon.k12.fl.us) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, October 17, 2000 at 11:33:21 ---------------------------------------------------------------------------
Email: crowj-at-mail.leon.k12.fl.us Name: students
School: The Academic Resource Center
State: Florida
Zip: 32304
Question: Students are working with bacteria and fungi and yeast. They want to know why yeast is killed at temperatures lower than body temperatures, yet bacteria can grow on human bodies with a much higher temperature (98.2 according to most sources is the average body temp).
Hello List - Have I waited long enough to receive my proceedings volumes from the Philadelphia M&M meeting? If so, whom should I contact regarding "re-shipment"? Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 308 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax 301-402-0396
We have both technical and postdoctoral openings for transmission electron microscopists interested in studying biological macromolecules and cellular assemblies to high resolution using electron cryomicroscopy. The recent research emphasis of our laboratory has been on the molecular mechanisms underlying the assembly, organization, and dynamics of the cytoskeleton. Stable funding is available for these positions for 5 years. Salaries are negotiable.
The Department of Biological Sciences provides outstanding modern TEM facilities including Philips CM300-FEG, CM200-FEG, and EM420 electron cryo-microscopes dedicated to structural studies. Both the 300kV and 200kV field emission gun microscopes are outfitted with CCD cameras, 2k x 2k and 1k x 1k respectively, in addition to film cameras.
Purdue is home to over 50,000 students, faculty, and staff, and is located in West Lafayette, Indiana - approximately 1 hour northwest of Indianapolis and 2.25 hours southeast of Chicago. The area affords the advantages associated with a major university, while providing an affordable and attractive 'small town' environment in which to live.
The ideal candidates will be enthusiastic, self-motivated individuals with strong communication skills who are committed to applying an integrated structure-function approach to the study of biological macromolecules. Interested individuals should contact Dr. McGough by e-mail or phone. Formal applications for the technical positions should be made via the Personnel Services department http://www.adpc.purdue.edu/Personnel/job-home.htm.
Purdue is an equal access/equal opportunity university.
Amy M. McGough, Ph.D. Assistant Professor, Department of Biological Sciences and Editorial Board Member, FEBS Letters Purdue University West Lafayette, IN 47907-1392 E-mail: amcgough-at-bilbo.bio.purdue.edu Office: 765-496-7501; Lab: 765-496-7716 Secretary: 765-494-4954; Fax: 765-496-1189 CM200F: 765-496-2746; CM300F: 765-496-7508 http://bilbo.bio.purdue.edu/~amcgough/ Biophysics & Cell Biology Symposium: http://bilbo.bio.purdue.edu/~rbernal/
Aside from all the concrete, sand and aggregate issues, the column on our Cameca microprobe rests on inflatable "feet" that helps reduce vibrations as well. This of course is in addition to an isolation slab that it sits on that was poured first.
-----Original Message----- } From: Buckingham, Steve [mailto:sbuckingham-at-excellatron.com] Sent: Thursday, October 12, 2000 10:59 AM To: 'Microscopy-at-MSA.Microscopy.Com'
Hi All, I have a JSM 840 that I need to relocate soon. The new location is very near a railroad and interstate! Does anyone have information on the optimum thickness of a concrete slab to prevent vibrations? Thanks,
Steve Buckingham Excellatron Solid State LLC 1460 Roswell Street, Suite J Smyrna, GA 30080 phone 770 438 2201 fax 770 438 2118
Maybe the rubber pads might help a bit, but is there actually going to be a problem? The 840 has really good spring suspension anyway, I can't see that rubber pads would add significant isolation to what's there already.
Might be instructive to ask if anyone's actually experienced vibration sensitivity with an 840.
rtch
} } } Hi All, } I have a JSM 840 that I need to relocate soon. The new location is } very near a railroad and interstate! Does anyone have information on } the optimum thickness of a concrete slab to prevent vibrations? } Thanks, } } Steve Buckingham } Excellatron Solid State LLC } 1460 Roswell Street, Suite J } Smyrna, GA 30080 } phone 770 438 2201 } fax 770 438 2118 } } Steve, } } Have you tried something cheaper? When I was with The Johns Hopkins } University School of Medicine we were remodeling the lab. A new } darkroom sink for developing negatives was being put in right beside } the TEM. The plumbers had to drill for water lines and a sink drain } within 3-5 feet of the microscope. We lifted up each corner of the } microscope and installed 60 or 80 durometer(I think it was 80), } 4"x4"x1" rubber pads and could use the microscope without vibration. } The lab was located just below street
Phil Rutledge USDA/ARS 151 } Dixon Drive Chestertown, MD 21620 voice: 410.778.4136 or 2120 fax: } 410.778.4399 e-mail: rutledge-at-ars.usda.gov } } } } Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Dear List Members, } } We are trying to section by microtome samples of solution-cast PEG. PEG } is dissolved in water and THF and allowed to rest until it becomes a } viscous solution by the evaporation of the solvent. It is then put into } molds and under vacuum to further rid the material of solvent. Finally, } the PEG becomes solid. } } THE PROBLEM: The PEG moldings can not be sectioned because they are too } brittle and crumble in the chuck. } } Does anyone have a protocol for preparing PEG samples that does not lead } to this end? } } Thank you very much, } } Jennifer Taylor
Thanks for your interest. There are around 500-600 different species of yeast, many of them unrelated genetically. Some of these, such as some species of Sporobolomyces have temperature requirements that are below normal body heat (37C). Others, such as Candida albicans, are pathogenic on humans, with their optimum temperature at normal body heat. The bakers' yeast, Saccharomyces cerevisiae, can survive 40C for short periods, but has its growth optimum at around 27C. There are specially selected strains (especially used in brewing) which have other growth optima.
Whilst I do not know the details, I believe that bacteria have similarly varied optimal growth temperatures (e.g. the iron bacterium Gallionella at 20C, the yoghurt bacterium Streptococcus thermophilus at over 45C). I hope this helps.
Cheers
Stephan Helfer PhD Mycologist, Royal Botanic Garden Edinburgh
} Email: crowj-at-mail.leon.k12.fl.us } Name: students } } School: The Academic Resource Center } } State: Florida } } Zip: 32304 } } Question: Students are working with bacteria and fungi and yeast. They } want to know why yeast is killed at temperatures lower than body } temperatures, yet bacteria can grow on human bodies with a much higher } temperature (98.2 according to most sources is the average body temp). } } --------------------------------------------------------------------------- } } } }
Cheers
Stephan 0131 248 2865 FAX 248 2901 http://www.rbge.org.uk
I called on Monday regarding the missing Proceedings, and found that there were 3 mailing lists, and they are now working on the third list, so it could be an additional 2 weeks before the final volumes are received. Hang in there...
Larry
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Dr. Lawrence F. Allard Senior Research Staff Member High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 PO Box 2008 Oak Ridge, TN 37831-6064
PEG comes in different molecular weights. You might try a lower molecular weight PEG whcih would be less crumbly.
Also, John Wolosewick developed a technique to section biological tissue embedded in PEG. When I worked in his lab, we used to attach the PEG embedded tissue to aluminum stubs with dental wax and put the stub in the chuck of the microtome. That would prevent the chuck of the microtome from crushing your sample.
Joe Neilly Abbott Laboratories D-45M, AP31 200 Abbott Park Rd. Abbott Park, IL 60064-6202 voice: (847)-938-5024 fax: (847)-938-5027 e-mail: joe.neilly-at-abbott.com
jtaylor-at-stevens-tech.edu on 10/17/2000 09:21:39 PM Please respond to jtaylor-at-stevens-tech.edu To: Microscopy-at-sparc5.Microscopy.Com cc:
Dear List Members, } } We are trying to section by microtome samples of solution-cast PEG. PEG } is dissolved in water and THF and allowed to rest until it becomes a } viscous solution by the evaporation of the solvent. It is then put into } molds and under vacuum to further rid the material of solvent. Finally, } the PEG becomes solid. } } THE PROBLEM: The PEG moldings can not be sectioned because they are too } brittle and crumble in the chuck. } } Does anyone have a protocol for preparing PEG samples that does not lead } to this end? } } Thank you very much, } } Jennifer Taylor
At 8:23 PM -0400 10/17/00, Jennifer E. Taylor wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
The problem may be the molcular weight of PEG that you are using. Years ago, I used PEG to embed heart muscle for TEM, and if memory serves, we used a mixture of MW 10,000 and 6,000 to obtain a "sectionable" consistancy. Our source of information at that time was work by John Wolosowick (this was in the early-to-mid 1980's). A similar mixture may work for you. I'lm sorry I can't be more speciic, but that was work done at another institution, for a PI who has since passed away, so I don't have immediate access to the records. Try looking up John's work (It was Wolosowick & Keith Porter, I believe).
Good luck, Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
I'm planning to BSA-gold uptake into human cell-culture for TEM. Cell lines are grown in DMEM-F12. With the aim to study the ultrastructure of the endocytic system and determine the pH of the various organelles. Many methods state that they grew the cells overnight in serum-free media before starting the uptake. Does anybody know if this alters the morphology and/or pH of the cell?
Jennifer Jackson University of Natal, Pietermaritzburg South Africa email: jacksonj-at-nu.ac.za.
When I did some PEG work, a piece of the PEG embedded sample was glued to a blank epoxy block and then sectioned. There are several papers out there on the technique.
Polyethylene Glycol (PEG) Embedding and Subsequent De-embedding as a Method for the Structural and Immunocytochemical Examination of Biological Specimens by Electron Microscopy. Histake Kondo. 1984. J. Electr. Micros. Tech., 1:227-241.
The application of polyethylene glycol (PEG) to electron microscopy. J.J. Wolosewick. 1980. J. Cell Biol., 86:675-681.
Greg
"Jennifer E. Taylor" wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear List Members, } } } } We are trying to section by microtome samples of solution-cast PEG. } PEG } } is dissolved in water and THF and allowed to rest until it becomes a } } viscous solution by the evaporation of the solvent. It is then put } into } } molds and under vacuum to further rid the material of solvent. } Finally, } } the PEG becomes solid. } } } } THE PROBLEM: The PEG moldings can not be sectioned because they are } too } } brittle and crumble in the chuck. } } } } Does anyone have a protocol for preparing PEG samples that does not } lead } } to this end? } } } } Thank you very much, } } } } Jennifer Taylor
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
On Oct 10 I posted a request for digital imaging info on behalf of graphics prof Jonathan Lipkin (jlipkin-at-ramapo.edu). I've seen a reply from Warren Straszheim (thanks, Warren!) but I've discovered that in that time period I didn't receive an unknown quantity of incoming email (including my own posting) and have no idea if anyone else responded also. So if any of you posted a reply to Jonathan and assumed I'd been able to forward it to him, I'd appreciate it if you'd send it to me again privately.
Many thanks, Dee
*************************************************************** Dee Breger Mgr. SEM/EDX Facility Lamont-Doherty Earth Observatory 61 Route 9W Palisades, NY 10964 USA T: 914/365-8640 F: 914/365-8155
http://www.ldeo.columbia.edu/micro http://www.discovery.com/area/science/micro/micro1.html http://www.lsc.org/antarctica/front.html Journeys in Microspace (Columbia University Press, 1995)
I have 3 print processors looking for homes - 2 free, one (new) for some fee.
Check out details at MSA's surplus equipment site: http://www.msa.microscopy.com/SurplusEquipment/SurplusListings.html
Ann Hein Lehman EM Facility Manager Trinity College Hartford, CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman
Some time back Gatan made a ±60+ single hole, non-rotary specimen holder for the JEOL 200-CX. They are no longer in stock and I was hoping to find one, or the equivalent, gathering dust somewhere that I could buy and put to good use. Reply privately to {treese-at-marinebio.mbl.edu} . I would also be interested in any surplus single tilt holders for the JEOL that I could try to modify for ±60 tilt. Thanks!....Tom Reese "
Steve, The chief advantage to using water immersion lenses has to do with, as you surmised, the refractive indices in the optical system. Live cells are most happy in aqueous media, so therefore it is best to look at them in such an environment. Coverslip corrected water lenses have the advantage of eliminating oil from the system, and no-coverslip "dipping" lenses eliminate the gross errors that can occur when looking through the unpredictable surface of water (try to take a picture of a fish from a dock, then jump in the water and try an underwater photo).
An air-water interface produces problems similar to an air-glass interface. In the same way that an oil lens will optimize the image of a fixed prep, a water immersion lens will optimize observation of an aqueous prep. The resolution of your optical system is determined generally by the lowest refractive index. Therefore it is beneficial to eliminate air from the equation, but there is no advantage to using an oil lens with a water prep. An oil lens may have higher potential NA, say 1.4 at 60X rather than 1.2 for the water 60X, but the system NA is limited by the 1.33 refractive index of water, not the 1.515 refractive index of the oil. The highest potential NA of the water prep system is 1.33, just as an oil system is potentially 1.5, yet practical design concerns result in about a 1.2 limit for the water prep, 1.4 for the oil.
An advantage to using a coverslip corrected water lens, your case #1, is that the lens designers need only account for two refractive indicies when dealing with spherical aberration, and these two will always be in similar proportion. As these are live cells and somewhat unpredictable, we cannot always know how close they will be to the coverglass. Between the cell and the coverglass is water, and between the coverglass and lens is again water. It does not matter for the system whether the cell is very close to the coverslip or a little further away - there will just be a little more or less water on the objective side. Thus the optical path through water and glass is always the same. With oil immersion, the oil and water in the path will change depending on whether the cell is relatively near or far from the coverslip, as the objective moves closer or further from the coverglass. This can result in spherical aberration. A sensitive instrument like a confocal microscope is sensitive to these errors.
As for your case #2, non-coverslip corrected water lenses, or "dipping lenses", are crucial for live cell experiments where a number of devices may be manipulating or even connected to the cells. For one thing we have cells in a dish with a number of devices penetrating the water from above, giving an irregular surface. Additionally we have a sometimes significant amount of buffer above the cells, giving rise to working distance issues. A dipping lens typically has a narrow tapered end, teflon coated, and can be brought nicely into the buffer. The NA is typically a little lower than a coverslip objective but the needs for working distance (~2-3mm) and narrow profile outweigh the need for very high NA (0.9 vs. 1.2 for a 60X). A dry or oil lens simply will not perform in such conditions.
I hope this gives you a bit more background.
Regards, Bill Fester Olympus America Microscope Division - NY
} } Netters } } In upgrading our light microscopes, we are buying some new lenses for our } confocal scope and a brightfield scope. The salesforce is regaling us with } tales of improved resolution, clarity, brightness etc that comes with } spending double or triple the price for a standard oil immersion or dry } lens. Could someone please clarify, either on-line or off, what the } advantages and disadvantages of each of the following ( my texts on light } microscopy don't deal with them). Are they worth the extra dollars? } } 1) a water immersion lens for looking at cells suspended in buffer and } covered with a coverslip (water is used rather than oil between coverslip } and lens) These lenses typically have a lower numerical aperture than an } oil immersion lens. } } 2) a water dipping lens, used to examine cells in culture directly, without } a coverslip. I would expect the absence of refractive indice changes } (media/coverslip/air or oil) would explain their improved brightness. } } Thanks in advance for your input } } Steve Barlow } SDSU EM Facility } }
I am posting this request to help Paul, a graduate student here. If anyone can help him out, please respond to me.
Thanks, Jo Ann Moore Senior Biological Scientist Anatomy Department, College of Medicine University of South Florida Tampa FL
Hello folks -
I am in the process of refurbishing an old Aus-Jena microscope in our laboratory, and would like to be able to connect a video camera to the camera mount, but unfortunately it seems nearly impossible to find an appropriate J-mount adapter anywhere costing less than my left leg and right hand. I am hoping that one of the photomicroscopists around might have a spare one of these squirreled away in a drawer or cabinet somewhere that they wouldn't mind parting with. And if there's a disabled Aus-Jena scope around that could be available for parts, that'd be great as well.
Many thanks, Paul Jantzen Alzheimer's Research Laboratory
I have an Oxford/Link exL WDS/EDX (detector not included) system that I will gladly send to anyone for cost of shipping. The monitor and printer are still operational (or was the last time I could get the system to boot up), the rest does not work at all. I also have a monitor to a Tracor 2000 system is anyone needs it.
Phoebe Doss Manager Electron Microscope Lab Oklahoma State University
Good Morning, We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion Pump light goes OFF, and after another 10 min. the Scope shuts down. We checked & rechecked all the cables connections, didn't help. ANY IDEAS!!!!! Thanks Chuck
} We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump } Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion } Pump light goes OFF, and after another 10 min. the Scope shuts down. } We checked & rechecked all the cables connections, didn't help. } ANY IDEAS!!!!!
Here's one - clean the penning gauge and see if it behaves.
Aloha, Tina
http://www.pbrc.hawaii.edu/bemf/microangela
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
{ { Subj: HELP WITH JEOL 100CX II Date: 10/19/00 6:26:30 PM US Mountain Standard Time From: buiocchi-at-astro.ocis.temple.edu (Chuck Buiocchi) To: Microscopy-at-sparc5.microscopy.com
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Good Morning, We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion Pump light goes OFF, and after another 10 min. the Scope shuts down. We checked & rechecked all the cables connections, didn't help. ANY IDEAS!!!!! Thanks Chuck
I haven't worked with the 100 CX but I am familiar with the JEOL way of doing things.
The diffusion pump LED is a warning lamp (assuming the usual JEOL way) and probably indicates that the cooling water or diff pump power is off. Check to see if the diffusion pump is heating and that you have water flow. I suspect bad water flow is keeping the pump heater from being turned on. The other 10 minutes to shut down is just another JEOL safety circuit. If the vacuum system is stays in rough pumping mode, the scope turns off after 30 minutes.
Ron Vane XEI Scientific
-----Original Message----- } From: Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
} We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. } Pump Light comes on for the INITIAL WARM-UP, But after ~ 20min, the } Diffusion Pump light goes OFF, and after another 10 min. the Scope } shuts down. We checked & rechecked all the cables connections, didn't } help. ANY IDEAS!!!!!
Sounds to me as if a connection to one of the diff pump heaters has broken. The wires become brittle from the heat and a bump while you were moving it make have broken one of them. A more costly possibility is that one of the heaters may have expired.
Regards
Rob
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa **
Hi Chuck, For the luck of details in your description- is the cooling water runs properly through all circuits? Both diff. pumps, electronics, lenses? Perhaps some line(s) (partially) clogged? Check 2 solenoid water valves on the water hoses on the floor behind the TEM. Are the wires broken? Those are easy to damage during the move. Feel sides of the diff. pumps and electronics return water line 15 minutes after start up. Are they getting hot? Flowmeter in the water line never hurts...
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax
This message is made of 100% recycled electrons. -----Original Message----- } From: Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
Dear Chuck, This could be a cooling problem; first DP. overheats and trips off, then a part of electronics - lens coils or power supply (whatever is cooled in your scope) -this would shut down the scope completely.
Good luck! Dan
Dan Kaszubski Micro-Time Co. Olathe,KS
Good Morning, We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion Pump light goes OFF, and after another 10 min. the Scope shuts down. We checked & rechecked all the cables connections, didn't help. ANY IDEAS!!!!! Thanks Chuck
I had a similar problem recently (SEM) with a JSM 35C, it pumped for 20-30 minutes and then cut out. Hints: 1. make sure the water flow is ok 2. make sure the diff pumps are getting hot 3. make sure the RP belts are tight. My JSM problem was a loose RP belt on the pump which 'roughs' the column - the pre-vac. in the column wasn't good enough. A new belt cured it.
But I still have the same problem on a 200CX TEM and it's none of the above - awaiting help from the answers to your original question! Let me know of any good ones you might get off-line, please!
Keith Ryan Marine Biological Association Plymouth UK
{ { { Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu} 10/20 4:23a } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Good Morning, We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion Pump light goes OFF, and after another 10 min. the Scope shuts down. We checked & rechecked all the cables connections, didn't help. ANY IDEAS!!!!! Thanks Chuck
} } } Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu} 10/20/00 00:19 AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Good Morning, We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion Pump light goes OFF, and after another 10 min. the Scope shuts down. We checked & rechecked all the cables connections, didn't help. ANY IDEAS!!!!! Thanks Chuck
Chuck,
I used a 100 CX and when thsat happened to me, it was usually the top diff pump heater. They go bad after a while. In 13 years I've only replaced the bottom diff pump heater 1 time. I've never used a CXII so I don't know if it has 2 diff pumps. Hope this helps.
We had a similar problem recently, but it turned out to be my fault, rather than the scope's. When you said you had just moved the scope, it rang a bell...
I didn't move the scope, but I rearranged the position of its water chiller. A couple days later, the scope was shutting down and the chiller was laboring mightily with a water temp of over 100 F. I checked the water level and refrigerant level and internal water flow in the chiller tank and all seemed well. So I called the refrigeration guy.
After looking around a bit, the first thing he says is, "Did you move this recently?" I said yes, and he felt behind the chiller and found two hoses that had crimped when I moved it against the wall. Poor thing was hardly getting any outside cooling water flow.
The refrigeration guy was kind. He didn't make fun of me and he didn't charge me for the visit.
Good luck, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
-----Original Message----- } From: Chuck Buiocchi [mailto:buiocchi-at-astro.ocis.temple.edu] Sent: Thursday, October 19, 2000 5:46 PM To: Microscopy-at-sparc5.microscopy.com
Good Morning, We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion Pump light goes OFF, and after another 10 min. the Scope shuts down. We checked & rechecked all the cables connections, didn't help. ANY IDEAS!!!!! Thanks Chuck
Have you checked the compressed air pressure or the pressure switch. I don't have drawings but I assume that one is fitted.
Good luck, Ron
} } Chuck } } I had a similar problem recently (SEM) with a JSM 35C, it pumped for } 20-30 minutes and then cut out. } Hints: } 1. make sure the water flow is ok } 2. make sure the diff pumps are getting hot } 3. make sure the RP belts are tight. } My JSM problem was a loose RP belt on the pump which 'roughs' the } column - the pre-vac. in the column wasn't good enough. A new belt } cured it. } } But I still have the same problem on a 200CX TEM and it's none of the } above - awaiting help from the answers to your original question! Let } me know of any good ones you might get off-line, please! } } Keith Ryan } Marine Biological Association } Plymouth UK } } { { { Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu} 10/20 4:23a } } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Good Morning, } We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. } Pump } Light comes on for the INITIAL WARM-UP, But after ~ 20min, the } Diffusion } Pump light goes OFF, and after another 10 min. the Scope shuts down. } We checked & rechecked all the cables connections, didn't help. } ANY IDEAS!!!!! } Thanks } Chuck } =========================================================================== Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk Department of Materials, phone +44 (0) 1865 273701 University of Oxford, fax +44 (0) 1865 283333 Parks Road. Oxford. OX1 3PH. UK. ============================================================================
please find enclosed a summary of the first circular of the 5th Multinational Congress on Electron Microscopy, that will be held in Lecce (ITALY), on September 20-25 2001.
An overseas participation is strongly desired, and we hope that many scientists from all over the world will come to Italy in September 2001.
The congress is organized by the following societies:
Austrian Society for Electron Microscopy Croatian Society for Electron Microscopy Czechoslovak Society for Electron Microscopy Hungarian Society for Microscopy Italian Society for Electron Microscopy Slovenian Society for Electron Microscopy
The purpose of the congress is to provide an interdisciplinary forum where scientists, both in the materials science and in the biomedical research can present the most recent results of their activity.
Some of the topics which will be covered are:
Architecture of the nucleus and nuclear import and export, art, biomaterials, biosensors, environmental microscopy, cell and cell components, cell death in biology and pathology, chemical analysis and mapping, microscopy in human health, microscopy of nanostructural materials, microscopy of structural and functional materials, energy-filtered electron microscopy, instrumental development, investigations of thin films for sensor technology, microbiology, microscopy of semiconducting materials, parassitology, plant cell biolgy, remote microscopy, strain analysis, structure biology, structural determination and refining, viruses, teleoperation and computer assisted operation, nanotechnology, single molecule microscopy and manipulation.
You can find further information and eventually download the brochures (soon available) and also preregister at the web site:
www.mcem5.unile.it
The Congress contacts are:
Laboratory of Comparative Anatomy and Cytology Dept. of Biology University of Lecce Via per Monteroni, 73100 Lecce, Italy Phone: +39 0832 320657 (855) Fax: +39 0832 320654
Institute for the study of new Materials for Electronics University of Lecce Via per Arnesano, 73100 Lecce, Italy Phone: +39 0832 322362 Fax: +39 0832 325299
The JEOL scopes have a heat sensor that ensures the DP is hot. They sometimes have two sensors, one at the bottom of the DP, the hottest point, and one at the top of the DP the coolest point. My guess is during your move the sensor(s) came out of the clamp that holds it against the DP. Look for a cylinder that has two wires coming out of it and find the metal band or clamp where it fits into. I hope this helps!
Bill
_______________________________
Bill Carmichael Electron Microscopy Faculty
Madison Area Technical College 3550 Anderson St. Madison, WI 53704 608-243-4309
This e-mail came in to the MSA Business Office. If you sell light microscopes you will want to respond directly to the writer. Please do not copy the listserver.
Ron
} } } } } }
To whom this may concern: I am Karim El Masry, Assistant Brand Manager on Haircare products in Procter & Gamble Egypt.
I was wondering if you could help me find video microscopes that magnify up to x200. I will be using the microscopes in one of my programs demonstrating the video microscopes capabilities.
Please advise with the company producing or supplying them. I will need at least a good 50 microscopes within the next two weeks.
Hope to hear from you very soon. Best Regards, Karim +202 394 7609
{elmasry.k-at-pg.com}
} } } } } } } }
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
IBM Analytical Services; http://www.chips.ibm.com/services/asg
Dear List, Does anyone out there know where I can order parts for my LKB Ultrotome V? I need to order new chucks and can't find a dealer who deals with the LKB's. Are parts available anymore? A textbook listed Cambridge Instruments as a supplier. Anyone have a phone, e-mail or address? Thanks for your help, Jo Dee
-- Jo Dee Fish Coordinator of Electron Microscopy Cell Analysis Facility The Burnham Institute 10901 N. Torrey Pines Rd. La Jolla, CA 92037 (858)646-3100 ext. 3620
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The following is a brief note from a graduate student who is going to try in situ localization on plant tissue for light microscopy. Any help you can give her is appreciated. Send replies to me and I will forward to her. Thanks in advance for your help. Debby ------ I am planning in situ hybridization using ribo-probes labelled with DIG with maize and rice tissues (coleoptiles, young leaves, roots and root tips). I am afraid that some of the organs like the root tips are very small to be embedded in paraffin and still be able to get nice cross sections. Is there any other resin that I can try? Also the fixative that I planned to use consists of 50% methanol, 5% acetic acid, and 2% formaldehyde (40%). Do you have other suggestions for a fixative. I would appreciate any protocols which may help in this project. Claudia Elena Vergara
---- Debby Sherman Phone: 765-494-6666 Life Science Microscopy Center FAX: 765-494-5896 c/o Dept.of Botany & Plant Path. E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id SAA12359 for dist-Microscopy; Fri, 20 Oct 2000 18:47:40 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id SAA12347 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 20 Oct 2000 18:47:09 -0500 (CDT) Received: from [206.69.208.21] (mac21.zaluzec.com [206.69.208.21]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id SAA12340 for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 20 Oct 2000 18:46:58 -0500 (CDT) X-Sender: zaluzec-at-ultra5.microscopy.com Message-Id: {v03130300b61689b97429-at-[206.69.208.21]} Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-Priority: 1 (Highest)
Good Morning Everyone, First let me say THANK YOU, to all who responsed to my earlier message, greatly appreciated!!! However some NEW additional info. The pump is HEATING up, but the Scope dies after it switches over to pump out the colum. Any Ideas??? Thanks again, Chuck
Listers, Anyone out there with a spare vac power supply & vac controller board for a JSM 35 SEM that they would be willing to sell? JEOL wasn't much help. Please reply off line, either by email or phone. Thanks in advance. Gary M. Easton Scanners Corporation 410-857-7633 x102
A colleague's student came and asked me how to prepare a ceramic powder for TEM observation. As I understand it, the powder is composed of alumina particles, average size 90 microns. The particles are very porous, with holes/pores ranging in size from around 1 to 30 microns. Their aim is to show the presence of internal pores. And yes they have done SEM on fractured particles, but they have been asked to do TEM as well.
Since these samples are rather different from what I generally work with, I'm hoping that someone can give us some advice on the specimen prep.
Many thanks in advance for any help you can give us.
Gill
Dr. Gillian M. Bond Department of Materials & Metallurgical Engineering New Mexico Tech Socorro, NM 87801
Leica may be able to help you Their US and UK addresses are copied below
The following web site has these and other microscopy vendor addresses, phone numbers, emails and links to their web pages
http://www.kaker.com/mvd/vendors.html Chris
Leica Instruments, Inc. 111 Deer Lake Road Deerfield, IL 60015 USA Tel: 800 248 0123, 708 405 0123 Fax: 708 405 8139 Leica UK Ltd Davy Avenue Knowlhill, Milton Keynes MK5 8LB UK Tel: +44 (0) 1908 24 62 46 Fax: +44 (0) 1908 60 99 92
Date sent: Fri, 20 Oct 2000 13:03:05 -0700 } From: Jo Dee Fish {jofish-at-burnham-inst.org} To: Microscopy-at-sparc5.microscopy.com
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
Gillian M. Bond wrote: ================================================================ A colleague's student came and asked me how to prepare a ceramic powder for TEM observation. As I understand it, the powder is composed of alumina particles, average size 90 microns. The particles are very porous, with holes/pores ranging in size from around 1 to 30 microns. Their aim is to show the presence of internal pores. And yes they have done SEM on fractured particles, but they have been asked to do TEM as well.
Since these samples are rather different from what I generally work with, I'm hoping that someone can give us some advice on the specimen prep.
Many thanks in advance for any help you can give us. ================================================================== We have been preparing these types of samples in our own laboratory for a number of years for our commercial clients. We have found that such samples ,
1] Have to be vacuum embedded in order to make sure all the internal pores are infiltrated with the resin,
2] Diamond knife ultramicrotomy is always required, because glass knives just won't "cut" it, and
3] We prefer our own SPI-Pon™ 812 resin (although at least some of the other "Epon substitute" resins should work just as well), in part because you have the possibility of varying the hardness of the final resin through a considerable range, a feature sometimes important in terms of ending up with a block with the "right" properties (e.g. hardness).
4] If these 90 µm particles were formed in situ, and not as the result of grinding, then they should be sputter coated with (preferably) Pt but Au will do if Pt is not available, prior to embedding, so that once in the TEM, one would always be able to keep track of the original outside surfaces, since this is the way one can determine the presence of a skin (of different morphology).
Contrast can be a problem for resolving the pores of the smallest dimensions , so one would probably be wise to think about the use of "holey" support films. We prefer our own SPI Materials Science Diamond Knives for this type of work because these kinds of samples will immediately impart to the knife edge the kinds of fine striations one works so hard to get out in the production of a more expensive "life science" diamond knife. In other words , using a new life science knife on these kinds of samples (in our opinion) is quite wasteful economically.
Disclaimer: SPI Supplies offers the embedding resins, diamond knives and holey support films for doing this kind of work. We also provide this kind of laboratory service for those not set up or wanting to do their own sample preparation.
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
} } } Good Morning Everyone, } First let me say THANK YOU, to all who responsed to my earlier } message, greatly appreciated!!! However some NEW additional info. } The pump is HEATING up, but the Scope dies after it switches over to } pump out the colum. Any Ideas??? Thanks again, Chuck } } }
Is that after starting to evacuate via the rotary pump, or later, when it switches over to the DP?
And does it switch off as soon as it starts the column evacuation, or after a while?
Maybe you've got such a massive air leak that the watchdog senses that there's not sufficient vacuum backing for the DP(s).
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Hello folks, I am looking for a used microtome which will work well in sectioning resin (1-3 micron). A manual microtome would be fine, especially if is a good bargain. Thanks!
Tonino Traini CDT-MDT-DDS Electron Microscopy Center Ud'A University * Tel: (871) 3554143 * Fax: (735) 90514 * E-mail: ttrain-at-tin.it
} The pump is HEATING up, but the Scope dies after it switches } over to pump out the colum. Any Ideas???
There are two diff pumps - are they both heating?
If both are heating I think the most likely possibility, as mentioned by others here in response to your original enquiry, is overheating of the diff pumps because of a water cooling problem. On the other hand, if the instrument turns off at or very soon after it tries to switch over to high vacuum, and if the diff pumps are hot but not overheating, then it sounds as if the rough pumping by the rotary pump has been inadequate. Is there any pumping noise from the rotary pumps? It may be that one of the hoses was not properly re- connected after the move, or one of the hoses may be leaking. They perish after a while.
Good luck.
Rob
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa ** ------- End of forwarded message -------
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa **
Email: ed_bachmann-at-unc.edu Name: Ed Bachmann School: University of North Carolina at Chapel Hill
Question: I am looking for a high quality new or used stereoscopic dissecting scope for examining mosses. I would like as high a magnification as I can get consistent with very good resolution. Is there anyplace I can find unbiased reviews or comparisons of brands and models? Can you comment on the use of adaptor lenses that attach to the objective lens to boost magnification. I've been told they cause image degradation (loss of resolution?). Thank you.
We have begun using a Gatan Bio-Scan digital camera on our LEO EM910 and we like it very much. I would like to improve on this camera's capabilities by increasing its spatial resolution. One method I've heard of and that is used in some digital cameras is to acquire multiple images at sub-pixel spacing and then recombine these into one image. My microscope has the capability of shifting the image in any direction in sub-pixel units. Has anyone tried this, or does anyone know where I can get information on how this might be done?
_____________________________________________________________ C. Robert Bagnell Jr., Ph.D., Res.Assoc.Prof. Microscopy Services Laboratory Department of Pathology & Laboratory Medicine CB #7525 UNC-CH, Chapel Hill, N.C. 27599 ph 919-966-2413 fx 919-966-6718 http://www.pathology.med.unc.edu/path/microscopy/welcome.htm
I was talking to a friend of mine this morning about what software he should have to do digital imaging. Reflexively I said PhotoShop and was asked how he would use it. I told him the standard things - contrast enhancement, color matching across different media, annotation, resizing, etc. His response was that for the price was that all it did (a little naivety but a fair question) . You know, sometimes you get in a rut and it set me to wondering how other people are using PhotoShop? It's not like I'm selling the software but I'd like to be able to give a better answer before telling someone they should plunk down that much money. Aside from image analysis plug-ins (which I had forgotten about when we were talking) how else is PhotoShop being used - anything fancy to justify the cost?
We are trying to track down the location of "Cambridge Image Technology," manufacturers of cathodoluminescence stages for optical microscopes. Can anyone help? Thanks in advance,
Mati
Mati Raudsepp, Associate Professor (Hon.) Dept. of Earth & Ocean Sciences 6339 Stores Road The University of British Columbia Vancouver, BC V6T 1Z4
You should add the ability to do montaging with layers, selecting and adjusting contrast ranges, selecting edges. you also have the capability of using John Russ' Image Processing Toolkit or Provea Pro to do relatively inexpensive image analysis.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
} -----Original Message----- } From: Bill Miller [mailto:microbill-at-mohawk.net] } Sent: Monday, October 23, 2000 5:25 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: PhotoShop usage } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html } } } -------------------------------------------------------------- } ---------. } } } I was talking to a friend of mine this morning about what software he } should have to do digital imaging. Reflexively I said } PhotoShop and was } asked how he would use it. I told him the standard things - contrast } enhancement, color matching across different media, } annotation, resizing, } etc. His response was that for the price was that all it did } (a little } naivety but a fair question) . You know, sometimes you get } in a rut and it } set me to wondering how other people are using PhotoShop? } It's not like } I'm selling the software but I'd like to be able to give a } better answer } before telling someone they should plunk down that much } money. Aside from } image analysis plug-ins (which I had forgotten about when we } were talking) } how else is PhotoShop being used - anything fancy to justify the cost? } } Bill Miller } }
One of the reasons that Photoshop is in such widespread use may be Adobe's sales policy. Like Apple years ago, they sell to Academic Institutions at very greatly discounted prices, so that for us we aren't "plunking down that much money" - in fact, the cost to us of Photoshop is competitive with far less able program suites. As a result, all our graduates go off into their jobs and immediately buy Photoshop!
In reality, though, I would still consider Photoshop because it has everything I could possibly need (other, cheaper packages are almost always deficient in some way or another), because it will handle 16 bit images, because its user interface is not frilled up but is fully accessible, because of the extensive file format support, and so on, as well, of course, as the plug-ins. Although Photoshop has power that I will never ever use, I'm also surprised at how often I use some feature that I would never have considered as important, if I did a feature by feature price-performance evaluation of cheaper products.
Tony Garratt-Reed
At 10:24 AM 10/23/2000 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
** Anthony J. Garratt-Reed ** MIT Room # 13-1027 ** 77 Massachusetts Avenue ** Cambridge, MA 02139-4307 ** USA ** ** Phone: (+) 1-617-253-4622 ** Fax: (+) 1-617-258-6478 **
This is a re-post; please excuse us if you've seen it before.
Also, please respond to Dr. Spector (e-mail given in post), not to the poster...although I will forward any messages I receive on to him. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Microscopy Core Technician -
Cold Spring Harbor Laboratory is seeking an experienced and responsible microscopy technician for the laboratory's core biological microscopy facility. The individual should have practical expertise in transmission electron microscopy, confocal and widefield fluorescence microscopy, digital imaging, and microinjection. The successful candidate will be involved in designing and carrying out experimental protocols for users, training individuals in the use of various microscopes, and aligning microscopes and keeping the facility operating at an efficient and high level of productivity. Interested individuals should send their resume, including a description of their expertise and the names and addresses of 3 references to: Dr. David L. Spector, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724, email: spector-at-cshl.org
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
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One nice action we do with PhotoShop is an automated process that compresses a large tiff file or similar into an e-mailable jpeg. In the automated batch process, it can compress a whole folder of large files in a matter of a minute or two.
-----Original Message----- } From: Bill Miller [mailto:microbill-at-mohawk.net] Sent: Monday, October 23, 2000 2:25 AM To: Microscopy-at-sparc5.microscopy.com
I was talking to a friend of mine this morning about what software he should have to do digital imaging. Reflexively I said PhotoShop and was asked how he would use it. I told him the standard things - contrast enhancement, color matching across different media, annotation, resizing, etc. His response was that for the price was that all it did (a little naivety but a fair question) . You know, sometimes you get in a rut and it set me to wondering how other people are using PhotoShop? It's not like I'm selling the software but I'd like to be able to give a better answer before telling someone they should plunk down that much money. Aside from image analysis plug-ins (which I had forgotten about when we were talking) how else is PhotoShop being used - anything fancy to justify the cost?
} } We are trying to track down the location of "Cambridge Image } Technology," manufacturers of cathodoluminescence stages for } optical microscopes. Can anyone help? Thanks in advance, } } Mati
Don't know that one, but we've been happy with the Luminoscope we got from Premier American Technologies, PA, phone 814/867-8600.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Easy. Take multiple images with each having some amount of overlap. Then feed these images into Visual Stitcher Pro ($50) and voile, one single big image.
gary
At 07:12 AM 10/23/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi Listers: Since all of us are heavily involved with the use of the internet, I thought this message I just received from Prof. Galler, one of the true computer experts here at the Univ. of Michigan, and one of the poeople who has been deeply involved in the development of our computer system, might be of interest to you. It not only sheds light on some of the political aspects of the development of the internet, but also shows how risky it can be to deal with news media.
I know this has some political overtones (and perhaps undertones, too) However, I think it is also of importance for all of us who use the Internet to have accurate information about it's development from reliable sources. Wil Bigelow - - - - - - - - - - - - - - - - - - -
} X-Sender: galler-at-g.imap.itd.umich.edu (Unverified) } Mime-Version: 1.0 } Date: Sat, 21 Oct 2000 11:00:26 -0400 } To: coe-fac-staff-at-umich.edu } From: "Bernard A. Galler" {galler-at-umich.edu} } Subject: Setting the record straight ... } Status: } } Many of us recently saw the excellent statement by Vint Cerf and Bob Kahn } on Gore's "Inventing the Internet" claim (appended below). I asked for and } received permission from Vint to send it to our local paper, the Ann Arbor } News. When I sent it to them, I added a preface which said: "I am } submitting this important Letter to the Editor, with permission. It was } written by two people who are generally regarded in the computer industry } as the real inventors of the Internet." } } Unfortunately, they did publish "my letter" without my preface and without } the first half of the Cerf/Kahn statement, but with my name at the bottom. } Not only did they remove the validation of the statement, since there is no } longer any mention of the real authors, but they cast me in the role of } plagiarist. I have received several email messages and phone calls } congratulating me on my letter and my excellent writing! } } You may be sure that I will visit the Ann Arbor News when I return to Ann } Arbor on Monday. } Bernie } =================================================================== } Al Gore and the Internet } } By Robert Kahn and Vinton Cerf } Al Gore was the first political leader to recognize the importance of the } Internet and to promote and support its development. } } No one person or even small group of persons exclusively "invented" the } Internet. It is the result of many years of ongoing collaboration among } people in government and the university community. But as the two people } who designed the basic architecture and the core protocols that make the } Internet work, we would like to acknowledge VP Gore's contributions as a } Congressman, Senator and as Vice President. No other elected official, to } our knowledge, has made a greater contribution over a longer period of time. } } Last year the Vice President made a straightforward statement on his } role. He said: "During my service in the United States Congress I took the } initiative in creating the Internet." We don't think, as some people have } argued, that Gore intended to claim he "invented" the Internet. Moreover, } there is no question in our minds that while serving as Senator, Gore's } initiatives had a significant and beneficial effect on the still-evolving } Internet. The fact of the matter is that Gore was talking about and } promoting the Internet long before most people were listening. We feel it } is timely to offer our perspective. } } As far back as the 1970s Congressman Gore promoted the idea of high speed } telecommunications as an engine for both economic growth and the } improvement of our educational system. He was the first elected official } to grasp the potential of computer communications to have a broader impact } than just improving the conduct of science and scholarship. Though easily } forgotten, now, at the time this was an unproven and controversial } concept. Our work on the Internet started in 1973 and was based on even } earlier work that took place in the mid-late 1960s. But the Internet, as we } know it today, was not deployed until 1983. When the Internet was still in } the early stages of its deployment, Congressman Gore provided intellectual } leadership by helping create the vision of the potential benefits of high } speed computing and communication. As an example, he sponsored hearings on } how advanced technologies might be put to use in areas like coordinating } the response of government agencies to natural disasters and other crises. } } As a Senator in the 1980s Gore urged government agencies to consolidate } what at the time were several dozen different and unconnected networks into } an "Interagency Network." Working in a bi-partisan manner with officials } in Ronald Reagan and George Bush's administrations, Gore secured the } passage of the High Performance Computing and Communications Act in } 1991. This "Gore Act" supported the National Research and Education } Network (NREN) initiative that became one of the major vehicles for the } spread of the Internet beyond the field of computer science. } } As Vice President Gore promoted building the Internet both up and out, as } well as releasing the Internet from the control of the government agencies } that spawned it. He served as the major administration proponent for } continued investment in advanced computing and networking and private } sector initiatives such as Net Day. He was and is a strong proponent of } extending access to the network to schools and libraries. Today, } approximately 95% of our nation's schools are on the Internet. Gore } provided much-needed political support for the speedy privatization of the } Internet when the time arrived for it to become a commercially-driven } operation. } } There are many factors that have contributed to the Internet's rapid growth } since the later 1980s, not the least of which has been political support } for its privatization and continued support for research in advanced } networking technology. No one in public life has been more intellectually } engaged in helping to create the climate for a thriving Internet than the } Vice President. Gore has been a clear champion of this effort, both in the } councils of government and with the public at large. } } The Vice President deserves credit for his early recognition of the value } of high speed computing and communication and for his long-term and } consistent articulation of the potential value of the Internet to American } citizens and industry and, indeed, to the rest of the world. } } } Bernard A. Galler } E-mail: galler-at-umich.edu } Fax: 734-668-9998 }
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
Photoshop is used for all of the things mentioned, and that only scratches the surface of what is possible. In addition to the full blown graphics aspects, the current full versions of Photoshop (version 5.5 with 6.0 set to release any day now) include PDF support and Adobe ImageReady, an application for preparing images for web viewing. If you require basic image manipulation tools and Text capabilities, consider PhotoShop LE. Its $99 and has the most commonly used features of its big brother. A comparison of "LE" vs 6.0 is available at : http://www.adobe.com/products/photoshople/comparison.html
George Laing National Graphic Supply
Bill, It's not from the microscopist's point of view but there's an article in a magazine called Photo Electronic Imaging (PEI) that may help answer some of your questions about Photoshop. I really enjoy this magazine. The October issue gives lots of good info on Photoshop 6 (pages 16-17 show a 6.0 feature roundup). And, every month there's an article called Photoshop - Instant Expert by Ben Willmore. The web site is www.peimag.com.
Beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
NATIONAL INSTITUTES OF HEALTH Bethesda, Maryland Division of Bioengineering & Physical Science Supramolecular Structure & Function Lab.
Physical /life scientist (Biologist GS9/GS11/GS12) at BS or MS level with solid technical experience in thin-section transmission electron microscopy. Experience in advanced techniques in analytical electron microscopy and structural biology (cryosectioning, high-pressure freezing, and macromolecular preparation methods) is desirable, although on-the-job training is possible for an experienced and meticulous microscopist. PROOF OF U.S. CITIZENSHIP IS REQUIRED for this appointment.
For further information please contact:
Dr. Richard Leapman Division of Bioengineering & Physical Science Bldg. 13, Rm. 3N17 National Institutes of Health Bethesda, MD 20892 Tel: (301) 496-2599 FAX: (301) 496-6608 E-mail: leapman-at-helix.nih.gov
Applications (with curriculum vitae or federal employment form SF-171) should include the reference number ORS-00-0231, and should be post marked by November 7, 2000 and sent to:
Ms. Karen Harris National Institutes of Health ORS Human Resources Office 31 Center Drive Msc 2157 Bldg 31, Rm 4B41 Bethesda, MD 20892
Our health and safety officer wants to know if Uranyl Nitrate is used as an EM stain. Does anyone use Uranyl Nitrate? He did not tell me why he needs to know.
Thanks,
Ruth
*************************************** Ruth Yamawaki Department of Comparative Medicine Stanford University Stanford, CA 94305 ***************************************
At 12:05 PM 10/23/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Amazing and informative. I truly thought that the Internet was created by DARPA and BBN. I had no idea that Gore played such a pivotal role in its creation. When I was working with DARPA in the '70s, Gore's name never came up. I suppose it was because the lower level creators were just computer scientists and engineers.
Email: hma-at-tupphysiol1.bp.dal.ca Name: HuaLong Ma School: Dalhousie University
Question: I am reading a paper using BrdU staining the cell and observe the fluorescence micrograph and confocal micrograph, could you give some explainations about these techniques and what is special about them. By the way, what is " Camera lucida" ? thanks
I don't believe that one person may claim that he or she has invented/created the Internet. Internet is a result of join efforts of many people from many countries (for this reason it called INTERnet). As my knowledge, Internet was grew as a fast way to communicate between members of the scientific community at the beginning. Because of international nature of the scientific community, Internet was "INTER" from the very early steps. I have no idea how Al Gore's was involved in such spontaneous out of control process. Moreover, I do believe, that the beauty of the Internet is their "deregulation" and non-controlling by any government-related agency. This is my personal opinion.
Sergey
} Date: Mon, 23 Oct 2000 15:05:17 -0400 } From: Wil Bigelow {bigelow-at-engin.umich.edu} } Subject: Setting the record straight ... } X-Sender: bigelow-at-srvr5.engin.umich.edu } To: Microscopy Listserver {microscopy-at-sparc5.microscopy.com} } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Wil, With all due respect, no more political mesages. Bob Ruscica
Wil Bigelow wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi Listers: } Since all of us are heavily involved with the use of the internet, I } thought this message I just received from Prof. Galler, one of the true } computer experts here at the Univ. of Michigan, and one of the poeople who } has been deeply involved in the development of our computer system, might } be of interest to you. It not only sheds light on some of the political } aspects of the development of the internet, but also shows how risky it can } be to deal with news media. } } I know this has some political overtones (and perhaps undertones, too) } However, I think it is also of importance for all of us who use the } Internet to have accurate information about it's development from reliable } sources. } Wil Bigelow } - - - - - - - - - - - - - - - - - - - } } } X-Sender: galler-at-g.imap.itd.umich.edu (Unverified) } } Mime-Version: 1.0 } } Date: Sat, 21 Oct 2000 11:00:26 -0400 } } To: coe-fac-staff-at-umich.edu } } From: "Bernard A. Galler" {galler-at-umich.edu} } } Subject: Setting the record straight ... } } Status: } } } } Many of us recently saw the excellent statement by Vint Cerf and Bob Kahn } } on Gore's "Inventing the Internet" claim (appended below). I asked for and } } received permission from Vint to send it to our local paper, the Ann Arbor } } News. When I sent it to them, I added a preface which said: "I am } } submitting this important Letter to the Editor, with permission. It was } } written by two people who are generally regarded in the computer industry } } as the real inventors of the Internet." } } } } Unfortunately, they did publish "my letter" without my preface and without } } the first half of the Cerf/Kahn statement, but with my name at the bottom. } } Not only did they remove the validation of the statement, since there is no } } longer any mention of the real authors, but they cast me in the role of } } plagiarist. I have received several email messages and phone calls } } congratulating me on my letter and my excellent writing! } } } } You may be sure that I will visit the Ann Arbor News when I return to Ann } } Arbor on Monday. } } Bernie } } =================================================================== } } Al Gore and the Internet } } } } By Robert Kahn and Vinton Cerf } } Al Gore was the first political leader to recognize the importance of the } } Internet and to promote and support its development. } } } } No one person or even small group of persons exclusively "invented" the } } Internet. It is the result of many years of ongoing collaboration among } } people in government and the university community. But as the two people } } who designed the basic architecture and the core protocols that make the } } Internet work, we would like to acknowledge VP Gore's contributions as a } } Congressman, Senator and as Vice President. No other elected official, to } } our knowledge, has made a greater contribution over a longer period of time. } } } } Last year the Vice President made a straightforward statement on his } } role. He said: "During my service in the United States Congress I took the } } initiative in creating the Internet." We don't think, as some people have } } argued, that Gore intended to claim he "invented" the Internet. Moreover, } } there is no question in our minds that while serving as Senator, Gore's } } initiatives had a significant and beneficial effect on the still-evolving } } Internet. The fact of the matter is that Gore was talking about and } } promoting the Internet long before most people were listening. We feel it } } is timely to offer our perspective. } } } } As far back as the 1970s Congressman Gore promoted the idea of high speed } } telecommunications as an engine for both economic growth and the } } improvement of our educational system. He was the first elected official } } to grasp the potential of computer communications to have a broader impact } } than just improving the conduct of science and scholarship. Though easily } } forgotten, now, at the time this was an unproven and controversial } } concept. Our work on the Internet started in 1973 and was based on even } } earlier work that took place in the mid-late 1960s. But the Internet, as we } } know it today, was not deployed until 1983. When the Internet was still in } } the early stages of its deployment, Congressman Gore provided intellectual } } leadership by helping create the vision of the potential benefits of high } } speed computing and communication. As an example, he sponsored hearings on } } how advanced technologies might be put to use in areas like coordinating } } the response of government agencies to natural disasters and other crises. } } } } As a Senator in the 1980s Gore urged government agencies to consolidate } } what at the time were several dozen different and unconnected networks into } } an "Interagency Network." Working in a bi-partisan manner with officials } } in Ronald Reagan and George Bush's administrations, Gore secured the } } passage of the High Performance Computing and Communications Act in } } 1991. This "Gore Act" supported the National Research and Education } } Network (NREN) initiative that became one of the major vehicles for the } } spread of the Internet beyond the field of computer science. } } } } As Vice President Gore promoted building the Internet both up and out, as } } well as releasing the Internet from the control of the government agencies } } that spawned it. He served as the major administration proponent for } } continued investment in advanced computing and networking and private } } sector initiatives such as Net Day. He was and is a strong proponent of } } extending access to the network to schools and libraries. Today, } } approximately 95% of our nation's schools are on the Internet. Gore } } provided much-needed political support for the speedy privatization of the } } Internet when the time arrived for it to become a commercially-driven } } operation. } } } } There are many factors that have contributed to the Internet's rapid growth } } since the later 1980s, not the least of which has been political support } } for its privatization and continued support for research in advanced } } networking technology. No one in public life has been more intellectually } } engaged in helping to create the climate for a thriving Internet than the } } Vice President. Gore has been a clear champion of this effort, both in the } } councils of government and with the public at large. } } } } The Vice President deserves credit for his early recognition of the value } } of high speed computing and communication and for his long-term and } } consistent articulation of the potential value of the Internet to American } } citizens and industry and, indeed, to the rest of the world. } } } } } } Bernard A. Galler } } E-mail: galler-at-umich.edu } } Fax: 734-668-9998 } } } } Wilbur C. Bigelow, Prof. Emeritus } Materials Sci. & Engr., University of Michigan } Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; } Fx:734-763-4788; Ph:734-662-5237
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} } We are trying to track down the location of "Cambridge Image } Technology," manufacturers of cathodoluminescence stages for } optical microscopes. Can anyone help? Thanks in advance, } } Mati
Here is a link to the web page for Cambridge Image Technology Ltd.
_____________________________ Dr Su Sajip Characterisation Development Engineer IQE (Europe) Ltd. United Kingdom Cypress Drive, St. Mellons Cardiff CF3 OEG * Wales, UK
Bill, I use PhotoShop for: Brightness/contrast/gamma/colour balance adjustments Re-size, rotate and annotate Select colour channels from RGB images Background correction Distortion of images to show make small thickness variations more visible Fiducial markers (very handy, I have a marker for each mag of my optical microscopes, SEM and TEM) Getting rid of blemishes/unwanted features (should I own up to this?) Making false colour SEM images Making movie sequences And probably a few more things I can't think of at the moment. I've used it to process } 10,000 images over the last 3 years.
I would only add that although it is expensive when bought by itself, it can often be obtained much more cheaply as part of a package for a scanner or camera.
Richard
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I was talking to a friend of mine this morning about what software he } should have to do digital imaging. Reflexively I said PhotoShop and was } asked how he would use it. I told him the standard things - contrast } enhancement, color matching across different media, annotation, resizing, } etc. His response was that for the price was that all it did (a little } naivety but a fair question) . You know, sometimes you get in a rut and it } set me to wondering how other people are using PhotoShop? It's not like } I'm selling the software but I'd like to be able to give a better answer } before telling someone they should plunk down that much money. Aside from } image analysis plug-ins (which I had forgotten about when we were talking) } how else is PhotoShop being used - anything fancy to justify the cost? } } Bill Miller }
e-mail richard.beanland-at-marconi.com Tel. +44 1327 356363 Fax. +44 1327 356398 ============================================================== "The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone. Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."
this message is to advertise the 5th multinational congress on electron microscopy, that will be held in Lecce (Italy) from September 20 to September 25, 2001.
The Congress is organized by the microscopy societies of the following countries:
Austria Croatia Czech Rep Hungary Italy Slovak Rep Slovenia
The meeting is a forum for all scientists active in all fields of microscopy.
The topics covered will be:
Architecture of the nucleus and nuclear import and export, art, biomaterials, biosensors, environmental microscopy, cell and cell components, cell death in biology and pathology, chemical analysis and mapping, microscopy in human health, microscopy of nanostructural materials, microscopy of structural and functional materials, energy-filtered electron microscopy, instrumental development, investigations of thin films for sensor technology, microbiology, microscopy of semiconducting materials, parassitology, plant cell biolgy, remote microscopy, strain analysis, structure biology, structural determination and refining, viruses, teleoperation and computer assisted operation, nanotechnology, single molecule microscopy and manipulation
A large exhibition area will be available to manufacturers. Open labs and tutorials will be organized.
Information can be found at www.mcem5.unile.it, where brochures can be downloaded too, or directly writing to me (I am president of the congress (materials science section) together with Prof. L. Dini, (biomedical field)).
We espect and hope to have a large participation from all around the world. Hope to see you all in Lecce.
with all due respect to anyone, and keeping in mind that this listserver is mailny populated by Americans (so I belong to a minority, here, being Italian) I would like to espress my opinion on Robert Ruscica's message.
I strongly believe that internet is one of the few places where a human being can "still" express his own opinion on something.
Expressing an idea, reporting an opinion, is a way to express his own "freedom" and freedom is important to anyone.
Provided that anyone has the possibility of replying a message, of expressing a different opinion, of stating what he thinks too, I find that "discussing" in a positive way is always stimulating, and a high level auditorium, as this listserver is supposed to be, is a good place to discuss, as science and research are (unfortunately or fortunately) strictly related to politics.
} Email: hma-at-tupphysiol1.bp.dal.ca } Name: HuaLong Ma } School: Dalhousie University } } Question: I am reading a paper using BrdU staining the cell and observe the } fluorescence micrograph and confocal micrograph, could you give some } explainations about these techniques and what is special about them. By the } way, what is " Camera lucida" ? thanks }
HuaLong -
I can't help you much with your flourescence and confocal questions, but I do know what a Camera lucida is - it's a simple optical device which allows you to "project" and enlarge the image seen in a microscope onto a piece of paper, so that you can then draw it more accurately. It may be as simple as a tiltable mirror mounted on a pole with a base which also tilts, allowing the user to make whatever adjustments necessary to optimize the "projection". These devices have been around for a long time, (at least a hundred years, and probably much longer) and were often used in the original production of some of the fine, meticulous drawings one sees in antique texts in a wide variety of subjects. I wouldn't be surprised if some are still in use in anatomical and paleontological studies - sometimes a hand drawing is the best way to illustrate a certain feature (and since you need fingers to hold a pencil, such drawings may have been the first "digital" micrographs (sorry)). In my own former specialty, micropaleontology, Camera lucida's are still being used now and then, and they may even still be commercially available. I see you're at Dalhousie; if you ever want to have a look at one, I'd be pleased to show you ours (after wiping the dust off it); we're just across the bridge.
Frank Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada B2Y 4A2
(902) 426-4635
From root Tue Oct 24 07:27:37 2000 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id HAA19848 for dist-Microscopy; Tue, 24 Oct 2000 07:15:41 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id HAA19845 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Tue, 24 Oct 2000 07:15:10 -0500 (CDT) Received: from mercury.mis-hq.com (mercury.mis-hq.com [64.208.103.66]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id HAA19838 for {microscopy-at-sparc5.microscopy.com} ; Tue, 24 Oct 2000 07:14:59 -0500 (CDT) Received: from SETH (jupiter.mis-hq.com [64.208.103.70]) by mercury.mis-hq.com (8.9.3/8.9.3) with SMTP id HAA04815; Tue, 24 Oct 2000 07:13:50 -0500 Reply-To: {sethg-at-paxit.com}
Let me join on the bandwagon....unless we want to start posting "Bush for President" signs on this listserv, we ought to drop the clever, some might even say "subliminal", political agendas.
SethG
Wil, With all due respect, no more political mesages. Bob Ruscica
Wil Bigelow wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi Listers: } Since all of us are heavily involved with the use of the internet, I } thought this message I just received from Prof. Galler, one of the true } computer experts here at the Univ. of Michigan, and one of the poeople who } has been deeply involved in the development of our computer system, might } be of interest to you. It not only sheds light on some of the political } aspects of the development of the internet, but also shows how risky it can } be to deal with news media. } } I know this has some political overtones (and perhaps undertones, too) } However, I think it is also of importance for all of us who use the } Internet to have accurate information about it's development from reliable } sources. } Wil Bigelow } - - - - - - - - - - - - - - - - - - - } } } X-Sender: galler-at-g.imap.itd.umich.edu (Unverified) } } Mime-Version: 1.0 } } Date: Sat, 21 Oct 2000 11:00:26 -0400 } } To: coe-fac-staff-at-umich.edu } } From: "Bernard A. Galler" {galler-at-umich.edu} } } Subject: Setting the record straight ... } } Status: } } } } Many of us recently saw the excellent statement by Vint Cerf and Bob Kahn } } on Gore's "Inventing the Internet" claim (appended below). I asked for and } } received permission from Vint to send it to our local paper, the Ann Arbor } } News. When I sent it to them, I added a preface which said: "I am } } submitting this important Letter to the Editor, with permission. It was } } written by two people who are generally regarded in the computer industry } } as the real inventors of the Internet." } } } } Unfortunately, they did publish "my letter" without my preface and without } } the first half of the Cerf/Kahn statement, but with my name at the bottom. } } Not only did they remove the validation of the statement, since there is no } } longer any mention of the real authors, but they cast me in the role of } } plagiarist. I have received several email messages and phone calls } } congratulating me on my letter and my excellent writing! } } } } You may be sure that I will visit the Ann Arbor News when I return to Ann } } Arbor on Monday. } } Bernie } } =================================================================== } } Al Gore and the Internet } } } } By Robert Kahn and Vinton Cerf } } Al Gore was the first political leader to recognize the importance of the } } Internet and to promote and support its development. } } } } No one person or even small group of persons exclusively "invented" the } } Internet. It is the result of many years of ongoing collaboration among } } people in government and the university community. But as the two people } } who designed the basic architecture and the core protocols that make the } } Internet work, we would like to acknowledge VP Gore's contributions as a } } Congressman, Senator and as Vice President. No other elected official, to } } our knowledge, has made a greater contribution over a longer period of time. } } } } Last year the Vice President made a straightforward statement on his } } role. He said: "During my service in the United States Congress I took the } } initiative in creating the Internet." We don't think, as some people have } } argued, that Gore intended to claim he "invented" the Internet. Moreover, } } there is no question in our minds that while serving as Senator, Gore's } } initiatives had a significant and beneficial effect on the still-evolving } } Internet. The fact of the matter is that Gore was talking about and } } promoting the Internet long before most people were listening. We feel it } } is timely to offer our perspective. } } } } As far back as the 1970s Congressman Gore promoted the idea of high speed } } telecommunications as an engine for both economic growth and the } } improvement of our educational system. He was the first elected official } } to grasp the potential of computer communications to have a broader impact } } than just improving the conduct of science and scholarship. Though easily } } forgotten, now, at the time this was an unproven and controversial } } concept. Our work on the Internet started in 1973 and was based on even } } earlier work that took place in the mid-late 1960s. But the Internet, as we } } know it today, was not deployed until 1983. When the Internet was still in } } the early stages of its deployment, Congressman Gore provided intellectual } } leadership by helping create the vision of the potential benefits of high } } speed computing and communication. As an example, he sponsored hearings on } } how advanced technologies might be put to use in areas like coordinating } } the response of government agencies to natural disasters and other crises. } } } } As a Senator in the 1980s Gore urged government agencies to consolidate } } what at the time were several dozen different and unconnected networks into } } an "Interagency Network." Working in a bi-partisan manner with officials } } in Ronald Reagan and George Bush's administrations, Gore secured the } } passage of the High Performance Computing and Communications Act in } } 1991. This "Gore Act" supported the National Research and Education } } Network (NREN) initiative that became one of the major vehicles for the } } spread of the Internet beyond the field of computer science. } } } } As Vice President Gore promoted building the Internet both up and out, as } } well as releasing the Internet from the control of the government agencies } } that spawned it. He served as the major administration proponent for } } continued investment in advanced computing and networking and private } } sector initiatives such as Net Day. He was and is a strong proponent of } } extending access to the network to schools and libraries. Today, } } approximately 95% of our nation's schools are on the Internet. Gore } } provided much-needed political support for the speedy privatization of the } } Internet when the time arrived for it to become a commercially-driven } } operation. } } } } There are many factors that have contributed to the Internet's rapid growth } } since the later 1980s, not the least of which has been political support } } for its privatization and continued support for research in advanced } } networking technology. No one in public life has been more intellectually } } engaged in helping to create the climate for a thriving Internet than the } } Vice President. Gore has been a clear champion of this effort, both in the } } councils of government and with the public at large. } } } } The Vice President deserves credit for his early recognition of the value } } of high speed computing and communication and for his long-term and } } consistent articulation of the potential value of the Internet to American } } citizens and industry and, indeed, to the rest of the world. } } } } } } Bernard A. Galler } } E-mail: galler-at-umich.edu } } Fax: 734-668-9998 } } } } Wilbur C. Bigelow, Prof. Emeritus } Materials Sci. & Engr., University of Michigan } Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; } Fx:734-763-4788; Ph:734-662-5237
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Yes, Wil's posting definitely has political overtones. So does the timing of the posting just 2 weeks before a presidential election. Are we now going to be inundated by apologists for every outrageous claim made by the Democrat candidate? I don't think I'd have time to read all email displaying torturous mental gymnastics justifying all the prevarications. Save it for the ballot box and keep it off the listserver.
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Hi Listers: Since all of us are heavily involved with the use of the internet, I thought this message I just received from Prof. Galler, one of the true computer experts here at the Univ. of Michigan, and one of the poeople who has been deeply involved in the development of our computer system, might be of interest to you. It not only sheds light on some of the political aspects of the development of the internet, but also shows how risky it can be to deal with news media.
I know this has some political overtones (and perhaps undertones, too) However, I think it is also of importance for all of us who use the Internet to have accurate information about it's development from reliable sources. Wil Bigelow - - - - - - - - - - - - - - - - - - -
} X-Sender: galler-at-g.imap.itd.umich.edu (Unverified) } Mime-Version: 1.0 } Date: Sat, 21 Oct 2000 11:00:26 -0400 } To: coe-fac-staff-at-umich.edu } From: "Bernard A. Galler" {galler-at-umich.edu} } Subject: Setting the record straight ... } Status: } } Many of us recently saw the excellent statement by Vint Cerf and Bob Kahn } on Gore's "Inventing the Internet" claim (appended below). I asked for and } received permission from Vint to send it to our local paper, the Ann Arbor } News. When I sent it to them, I added a preface which said: "I am } submitting this important Letter to the Editor, with permission. It was } written by two people who are generally regarded in the computer industry } as the real inventors of the Internet." } } Unfortunately, they did publish "my letter" without my preface and without } the first half of the Cerf/Kahn statement, but with my name at the bottom. } Not only did they remove the validation of the statement, since there is no } longer any mention of the real authors, but they cast me in the role of } plagiarist. I have received several email messages and phone calls } congratulating me on my letter and my excellent writing! } } You may be sure that I will visit the Ann Arbor News when I return to Ann } Arbor on Monday. } Bernie } =================================================================== } Al Gore and the Internet } } By Robert Kahn and Vinton Cerf } Al Gore was the first political leader to recognize the importance of the } Internet and to promote and support its development. } } No one person or even small group of persons exclusively "invented" the } Internet. It is the result of many years of ongoing collaboration among } people in government and the university community. But as the two people } who designed the basic architecture and the core protocols that make the } Internet work, we would like to acknowledge VP Gore's contributions as a } Congressman, Senator and as Vice President. No other elected official, to } our knowledge, has made a greater contribution over a longer period of time. } } Last year the Vice President made a straightforward statement on his } role. He said: "During my service in the United States Congress I took the } initiative in creating the Internet." We don't think, as some people have } argued, that Gore intended to claim he "invented" the Internet. Moreover, } there is no question in our minds that while serving as Senator, Gore's } initiatives had a significant and beneficial effect on the still-evolving } Internet. The fact of the matter is that Gore was talking about and } promoting the Internet long before most people were listening. We feel it } is timely to offer our perspective. } } As far back as the 1970s Congressman Gore promoted the idea of high speed } telecommunications as an engine for both economic growth and the } improvement of our educational system. He was the first elected official } to grasp the potential of computer communications to have a broader impact } than just improving the conduct of science and scholarship. Though easily } forgotten, now, at the time this was an unproven and controversial } concept. Our work on the Internet started in 1973 and was based on even } earlier work that took place in the mid-late 1960s. But the Internet, as we } know it today, was not deployed until 1983. When the Internet was still in } the early stages of its deployment, Congressman Gore provided intellectual } leadership by helping create the vision of the potential benefits of high } speed computing and communication. As an example, he sponsored hearings on } how advanced technologies might be put to use in areas like coordinating } the response of government agencies to natural disasters and other crises. } } As a Senator in the 1980s Gore urged government agencies to consolidate } what at the time were several dozen different and unconnected networks into } an "Interagency Network." Working in a bi-partisan manner with officials } in Ronald Reagan and George Bush's administrations, Gore secured the } passage of the High Performance Computing and Communications Act in } 1991. This "Gore Act" supported the National Research and Education } Network (NREN) initiative that became one of the major vehicles for the } spread of the Internet beyond the field of computer science. } } As Vice President Gore promoted building the Internet both up and out, as } well as releasing the Internet from the control of the government agencies } that spawned it. He served as the major administration proponent for } continued investment in advanced computing and networking and private } sector initiatives such as Net Day. He was and is a strong proponent of } extending access to the network to schools and libraries. Today, } approximately 95% of our nation's schools are on the Internet. Gore } provided much-needed political support for the speedy privatization of the } Internet when the time arrived for it to become a commercially-driven } operation. } } There are many factors that have contributed to the Internet's rapid growth } since the later 1980s, not the least of which has been political support } for its privatization and continued support for research in advanced } networking technology. No one in public life has been more intellectually } engaged in helping to create the climate for a thriving Internet than the } Vice President. Gore has been a clear champion of this effort, both in the } councils of government and with the public at large. } } The Vice President deserves credit for his early recognition of the value } of high speed computing and communication and for his long-term and } consistent articulation of the potential value of the Internet to American } citizens and industry and, indeed, to the rest of the world. } } } Bernard A. Galler } E-mail: galler-at-umich.edu } Fax: 734-668-9998 }
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
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Colleagues....
I've already replied privately to a number of you but before it start to grow let's nip this one in the bud.
Unless the topic is Microscopy and Microanalysis related please keep tangential commentary off-line. Comments about the Internet and what any politican's may have or have not said has no direct bearing on our agenda.
I met a problem in our EDS system, we have a TX 1255 Nortan detector and 4pai system (DTSA software). The problem is even the input counts is high to 2000 to 3000 and the dead time is below 30%, the output counts is less than 200, and when the input is less than 300 and dead time is less than 30%, there is no out put. I checked the discriminator adjustment in the pulse processor, it is almost there. How can I adjust the system?
Regards!
J.G. Wang
Jinguo Wang Materials Characterization Laboratory 194 MRI Building 5-9285
On Mon, 23 Oct 2000 15:52:51 -0700 Ruth Yamawaki {Ryamawaki-at-cmexchange.stanford.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Ruth,
Uranyl nitrate is used to make Reynolds lead citrate, a widely used TEM post stain for ultrathin tissue sections. In this formula, the lead nitrate is chelated by citrate. Hope this is of some use
} Our health and safety officer wants to know if Uranyl Nitrate is used } as an EM stain. Does anyone use Uranyl Nitrate? He did not tell me } why he needs to know. }
Full-time position available for an Electron Microscopy/Histology Technician to work on a NSF funded research project on biological structural colors. Responsibilities include specimen preparation, observation, and imaging using transmission electron microscopy (TEM) and light microscopy. Additional opportunties for active participation in research and publication. Required qualifications: Bachelor's degree or equivalent in an appropriate field; previous experience in microscopy or histology; previous experience in laboratory research. Preferred qualifications: previous experience in specimen preparation and sectioning for TEM; previous experience operating transmission eletron microscopes; previous experience in fixation, sectioning, and staining for light microscope histology; previous experience in computer data base management; graduate degree in an appropriate field. Salary: $25,000 per year (full-time) with benefits. Send a CV, a written statement describing previous experience and qualifications, and the names addresses, phone, and email addresses of three professional references to: Dr. Richard O. Prum, Natural History Museum, Dyche Hall, University of Kansas, Lawrence, KS 66045-2454; (785) 864-3897; prum-at-ukans.edu. Review of applications begins Nov. 1, 2000 and will continue until position is filled. EO/AA Employer
------------------------------------------------------------------------------ -- Richard O. Prum Associate Professor, Department of Ecology and Evolutionary Biology Curator, Division of Ornithology KU Natural History Museum University of Kansas Lawrence, KS 66045 Phone: (785)864-3897 Fax: (785)864-5335 Email: prum-at-ukans.edu ------------------------------------------------------------------------------ --
} ... I said PhotoShop, and was asked how he would use it. } I told him the standard things - contrast } enhancement, color matching across different media, } annotation, resizing, etc. ... } ... } how else is PhotoShop being used - anything fancy to } justify the cost?
We use PS5 here for annotation and presentation of spacial elemental mapping (elemental x-ray maps), and average atomic number maps (backscatter electron images). There isn't better software for presenting this type of data in a "perceptually" uniform way. For example, if you have a grayscale elemental map which represents SiO2 from 0% to 100%, with most softwares and typical monitors the change from 20% to 30% isn't perceived the same as 70% to 80%, if perceived at all. Opening and converting such an image into a Photoshop gamma=2.2 working space makes this gradation perceptually uniform, and much better for presentation. The caveat here is the "conversion" when you do this ... that is, PS makes the simple opening of the file such an innocent process, the user may not notice his/her data has been modified. What was once a pixel value of '128' is now '183'!! The data is now useless for quantitative analysis. Make it a practice to keep (possibly even write-protect) your original image files, and put your PS projects in a separate directory.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
} Date: Mon, 23 Oct 2000 15:52:51 -0700 } From: Ruth Yamawaki {Ryamawaki-at-cmexchange.stanford.edu} } To: "'Microscopy-at-sparc5.microscopy.com'" {Microscopy-at-sparc5.microscopy.com} } Subject: Uranyl Nitrate } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Our health and safety officer wants to know if Uranyl Nitrate is used as an } EM stain. Does anyone use Uranyl Nitrate? He did not tell me why he needs } to know. } } Thanks, } } Ruth } } *************************************** } Ruth Yamawaki } Department of Comparative Medicine } Stanford University } Stanford, CA 94305 } *************************************** } Uranyl nitrate, like uranyl acetate and other uranium salts, is used as a stain in EM. The salt most frequently used is uranyl acetate because of its staining efficiency. Uranium has a heavy atomic nucleus (92) and is the heaviest metal used as a stain in EM. In tissue, it stabilizes nucleic acids and membranes when used prior to embedding. It is frequently used after sections are cut to "post stain" or further increase the contrast of membranes, etc., in sections. Uranyl salts, particularly acetate, are also used as negative stains for small things such as viruses and small cellular organelles in suspension. The stain darkens the background support and cracks and crevasses in the sample to add contrast. Uranyl salt solutions should be handled with care because they are radioactive. The alpha radiation is generally contained by glass (lead not necessary), but nonetheless, one should not leave dried pipets and droplets lying around to become airborne and possibly inhaled.
Hayat MA. Positive Staining for Electron Microscopy. 1975. Van Nostrand Reinhold, NY.
Hayat MA, Miller SE. 1990. Negative Staining. McGraw-Hill, NY.
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
I am currently trying to source an used heating holder, cooling holder and a power supply for use in an Hitachi H600 TEM. If you have either of these holders that are not in use and would like to sell them (or donate them to a University) please contact me to see if we can come to a mutually agreeable price.
Chas
Charlie Cooney Metallographer Dept. of Materials and Metallurgical Engineering Queen's University Nicol Hall Kingston, Ontario K7L 1N6
We are looking into purchasing a digital microscope camera for our Zeiss axioplan upright compound microscope. We are particularly interested in color cameras that can capture good fluorescent images in addition to brightfield images.
I would very much appreciate some suggestions regarding a good camera.
Thanks in advance.
Soumitra
************************************************************* Soumitra Ghoshroy Ph.D. Electron Microscopy Lab and Fluorescence Imaging Facility Box 3EML New Mexico State University Las Cruces, NM 88003 Tel: 505-646-3600 Fax: 505-646-5665 e-mail:sghoshro-at-nmsu.edu http://confocal.nmsu.edu/eml
} } Dear Listservers, } } with all due respect to anyone, and keeping in mind that this listserver is } mailny populated by Americans (so I belong to a minority, here, being } Italian) I would like to espress my opinion on Robert Ruscica's message. } } I strongly believe that internet is one of the few places where a human } being can "still" express his own opinion on something. } } Expressing an idea, reporting an opinion, is a way to express his own } "freedom" and freedom is important to anyone.
Of course it is. Of course the internet is a wonderful thing. Of course we all want to discuss our political philosophies (vote for Bush).
However, the internet is also a *big* thing. There are lots and lots of people discussing lots and lots of different things here. Not everyone needs to discuss all topics in every conversation.
Nader is an idiot.
It is not a restriction on any inalienable liberties for a group of people to assemble and say "Here, today, we have chosen to talk about microscopy. If you want to talk about Gore, feel free to go to the political group. If you want to talk about woodworking, there is a fine woodworking discussion group over there. If you want to talk about Dungeons and Dragons, there is a great D&D group across the hall." That part and parcel of the right of association, and it is just as important as a right of speech.
Bush is wonderful.
It is further inappropriate for someone to come in and say "I don't care what everyone has agreed to! I want to talk about Gore's policiy towards D&D--playing woodworkers, and I don't care if it *is* a microscopy group. *I* care about Gore and woodworking and you microscopists had just better sit down and listen!"
Buchanan is a Nazi.
That is inappropriate, and it is not an abrogation of liberty for folk to say "Well, I'd rather you didn't."
Gore is dangerous.
On a practical note, that is *why* we have mailing lists and news groups. Do you suggest that we do away with all of them and just have one large mailing list and one large newsgroup called misc.stuff? Of course not.
Gore bad. Bush good.
In large part, we have managed to avoid direct moderation of most newsgroups and mailing lists by agreeing to abide by self-selected and agreed upon rules of behavior. Refusing to abide by these choices will not result in greater "freedom," but will instead result in the opposite -- the increasing direct moderation and censorship of newsgroups as a last-ditch attempt to control spam and intrusion.
M.A. Hayat mentions the use of uranyl nitrate on page 34 of his book Positive Staining (c. 1975) as an electron stain. He says that it is soluble in water, ethanol, acetone, and ether, but not in benzene, toluene and chloroform. The aqueous solution can hold up to 56% of anhydrous salt (w/v) at 25 C. Uranyl nitrate solutions are more stable than are uranyl acetate solutions but its less efficient as a stain than the acetate.
I have never used it. Ask your saftey officer why he needs to know.
Gib Ahlstrand
} Responding to the message } from Ruth Yamawaki {Ryamawaki-at-cmexchange.stanford.edu} : } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Our health and safety officer wants to know if Uranyl Nitrate is used as an } EM stain. Does anyone use Uranyl Nitrate? He did not tell me why he needs } to know. } } Thanks, } } Ruth } } *************************************** } Ruth Yamawaki } Department of Comparative Medicine } Stanford University } Stanford, CA 94305 } ***************************************
Gib Ahlstrand Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu http://biosci.umn.edu/MIC/consortium.html
According to the Polysciences Catalog Uranyl Nitrate is a negative stain. Also a photopolymerizing reagent for the polymerization of acrylamide gels at low pH. I personally have no experience with the stuff, but would imagine that it is similar to uranyl acetate which is a commonly used negative or positive stain. Tim
Timothy G. Schneider Director of Electron Microscopy Department of Pathology Room 229 Jefferson Hall Thomas Jefferson University 1020 Locust St. Philadelphia Pa. 19107 215-503-4798 work 610-613-8170 cellular timothy.schneider-at-mail.tju.edu
Hi All, I am looking for a good replacement for D19b developer (for nuclear emulsion plates, as used in our X-ray radiography and topography images, no longer available). We are using Suprol at the moment, but would like something as good as the old stuff. Anyone have any ideas?
e-mail richard.beanland-at-marconi.com Tel. +44 1327 356363 Fax. +44 1327 356398 ============================================================== "The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone. Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."
Hi all: I am shopping around, looking for recommendation of glass suppliers for that could make a chamber for our CRC-100 magnetron sputter coater. The manufacturer wants alot for theirs and I am looking for alternative suppliers that this community might be aware of . Any suggestions would be greatly appreciated. Thanks in advance for your assistance.
Regards, Mike Coviello Lab Manager Unversity of Texas -at- Arlington
} -----Original Message----- } From: Berryhill, Bridgette } Sent: Tuesday, October 24, 2000 10:15 AM } To: Azizan, Azliyati; Barber, Janet; Chen, Lan; Gahagan, Jackie; } Govindraj, Prasanthi; Grimm, David; Hall,Laura; Hernandez, Dan; Howell, } Troy; Ingrassia, Angela; Kane, Brad; McKinnon,William; McPherson, } Charlene; Su, Phy-Huynh; Vallet, Noelle; Westling, Jennifer; 'David } Alexandrou'; 'Elyse Drossman'; 'Jane Dunlevy'; 'MaryBeth Colter'; 'Stacey } Kennedy' } Subject: FW: Easy way to help a good cause TOMORROW } } } From: Amy Wolfson } Sent: Monday, October 23, 2000 9:59 PM } To: Debby Greenberg; Marilyn Weisman; Michele Schointuch; Fran Silver; } Carol Bassett; Victoria Rennesund; Stephanie Shaw; Andrea Sands; Bridgette } Berryhill; Shari Hamilton; Sharon Bevis; Jane Birkhold; Darbi Bossman; } Jennifer Capouya; Cindy Daniels; Pat Fox; Bev Kinsey; Nancy Leeds; Wendy } McCoy; Ilene Beer; Sarah Shanks; Alina Simonds; Erin Stuart; Katya Bock; } Lowie Bock; Connie Zink } Cc: Mary DeLong } Subject: Fw: Easy way to help a good cause TOMORROW } } } } Subject: Easy way to help a good cause TODAY - Oct .24 } } } } } } } } } } } } Subject: FW: Breast Cancer & the NFL } } } } } } The NFL has chosen October 24, 2000 as the NFL's Breast Cancer } } } } } Awareness day. Every time someone logs onto www.NFL.com, the NFL } } will } } } } } donate $5 to the Susan G. Komen Breast Cancer Foundation. This } is } a } } } } simple } } } } } opportunity to learn about one of our fabulous National Series } } Sponsor } } } } of } } } } } the Komen Race for the Cure(R), as well as earn up to $50,000 for } the } } } } Komen } } } } } Foundation. Tell all your friends and co-workers on October 24 } to } } log } } } } on!! } } }
Photographic developers that are no longer available can often be made from scratch using just a few standard ingredients that are fairly readily available. It may be that Eastman Kodak would consent to give you the formula if asked, but if not, chances are pretty good that the formula is out there floating around somewhere in an old book, journal, or even in cyberspace.
I know that most people don't want to mess around with making their own developers, or don't feel secure using them, but if you're interested (and not in a huge hurry) I can check some sources to try and locate the formula. It is probably just a minor variant of regular Kodak D-19. I have a small collection of darkroom chemistry oriented literature, plus some good leads on where to ask.
Good luck, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
-----Original Message----- } From: Richard Beanland +44 1327 356363 [mailto:richard.beanland-at-marconi.com] Sent: Tuesday, October 24, 2000 12:38 PM To: Microscopy Listserver
Hi All, I am looking for a good replacement for D19b developer (for nuclear emulsion plates, as used in our X-ray radiography and topography images, no longer available). We are using Suprol at the moment, but would like something as good as the old stuff. Anyone have any ideas?
e-mail richard.beanland-at-marconi.com Tel. +44 1327 356363 Fax. +44 1327 356398 ============================================================== "The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone. Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."
Does anyone know of software that will do a batch conversion of the .IM_ image files captured using Link ISIS v. 3 Autobeam, to a standard format (eg. pcx, jpg, etc)?
Fiona ____________________ Dr Fiona Graham EM Unit, University of Natal, Durban South Africa
**** Remember ICEM-15 in 2002, is in Durban, South Africa **** www.icem15.com
Ruth, 0.01% Uranyl Nitrate is used as a local catalyst in the polymerization of methacrylates.
Hayat MA. Principles and Techniques of Electron Microscopy. 1970. Van Nostrand Reinhold, NY.
Hope this helps! Jo Dee
Sara Miller wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } On Mon, 23 Oct 2000, Ruth Yamawaki wrote: } } } Date: Mon, 23 Oct 2000 15:52:51 -0700 } } From: Ruth Yamawaki {Ryamawaki-at-cmexchange.stanford.edu} } } To: "'Microscopy-at-sparc5.microscopy.com'" } {Microscopy-at-sparc5.microscopy.com} } } Subject: Uranyl Nitrate } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Our health and safety officer wants to know if Uranyl Nitrate is used as an } } EM stain. Does anyone use Uranyl Nitrate? He did not tell me why he needs } } to know. } } } } Thanks, } } } } Ruth } } } } *************************************** } } Ruth Yamawaki } } Department of Comparative Medicine } } Stanford University } } Stanford, CA 94305 } } *************************************** } } } Uranyl nitrate, like uranyl acetate and other uranium salts, is used as a } stain in EM. The salt most frequently used is uranyl acetate because of } its staining efficiency. Uranium has a heavy atomic nucleus (92) and is } the heaviest metal used as a stain in EM. In tissue, it stabilizes } nucleic acids and membranes when used prior to embedding. It is } frequently used after sections are cut to "post stain" or further } increase the contrast of membranes, etc., in sections. Uranyl salts, } particularly acetate, are also used as negative stains for small things } such as viruses and small cellular organelles in suspension. The stain } darkens the background support and cracks and crevasses in the sample to } add contrast. Uranyl salt solutions should be handled with care because } they are radioactive. The alpha radiation is generally contained by } glass (lead not necessary), but nonetheless, one should not leave dried } pipets and droplets lying around to become airborne and possibly inhaled. } } Hayat MA. Positive Staining for Electron Microscopy. 1975. Van Nostrand } Reinhold, NY. } } Hayat MA, Miller SE. 1990. Negative Staining. McGraw-Hill, NY. } } Sara E. Miller, Ph. D. } P. O. Box 3712 } Duke University Medical Center } Durham, NC 27710 } Ph: 919 684-3452 } FAX: 919 684-3265
-- Jo Dee Fish Coordinator of Electron Microscopy Cell Analysis Facility The Burnham Institute 10901 N. Torrey Pines Rd. La Jolla, CA 92037 (858)646-3100 ext. 3620
} -Hi All, } I am looking for a good replacement for D19b developer (for nuclear emulsion plates, as used in our X-ray radiography and topography images, no longer available). We are using Suprol at the moment, but would like something as good as the old stuff. Anyone have any ideas? } } Richard } } ============================================================== } Richard Beanland, } Structural Analysis Lab, } Caswell Technology, } Caswell, } Towcester, } Northants NN12 8EQ } } e-mail richard.beanland-at-marconi.com } Tel. +44 1327 356363 } Fax. +44 1327 356398 }
How about mixing your own from scratch? I will see if I can dig up the formula tomorrow (Wed).
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
Just for your information: A camera Lucida is also called a Drawing Tube and even sometimes Une Chambre claire. It does not project the image observed thru the microscope but allowed to overimposed the image seen thru the microscope and a piece of paper located on the side of the microscope and at the same time the pencil on this paper. The brightness if the paper as the be the same as the image observed to see both field at the same time. Than one just have to follow the contour of the object to obtain the right drawing. It exist drawing tubes for Compond microscopes as well as stereo`s microscopes My two cents information Norm
----- Original Message ----- } From: Frank Thomas {thomasf-at-agc.bio.ns.ca} To: {Microscopy-at-sparc5.microscopy.com} ; {hma-at-tupphysiol1.bp.dal.ca} Sent: Tuesday, October 24, 2000 07:28
Folks we have some pretty definitive formularies for photographic chemicals here at the museum. Contact us off list with a wish list of developer recipes if you have not found anything.
thanks Ed Sharpe archivist for SMECC
{ { Subj: RE: D19b developer Date: 10/24/00 1:43:45 PM US Mountain Standard Time From: TindallR-at-missouri.edu (Tindall, Randy D.) To: richard.beanland-at-marconi.com ('Richard Beanland +44 1327 356363') CC: microscopy-at-sparc5.microscopy.com ('microscopy-at-sparc5.microscopy.com')
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
Richard,
Photographic developers that are no longer available can often be made from scratch using just a few standard ingredients that are fairly readily available. It may be that Eastman Kodak would consent to give you the formula if asked, but if not, chances are pretty good that the formula is out there floating around somewhere in an old book, journal, or even in cyberspace.
I know that most people don't want to mess around with making their own developers, or don't feel secure using them, but if you're interested (and not in a huge hurry) I can check some sources to try and locate the formula. It is probably just a minor variant of regular Kodak D-19. I have a small collection of darkroom chemistry oriented literature, plus some good leads on where to ask.
Good luck, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
} The caveat here is the "conversion" when you do this ... that is, PS } makes the simple opening of the file such an innocent process, the } user may not notice his/her data has been modified. What was once a } pixel value of '128' is now '183'!!
I don't want to put down Photoshop, especially since we are producing a competing product, but I think this remark points to one problem that many "photo" applications have -- they do not treat the intensity as scientific data. I may be mistaken, but I think Photoshop is conceptually a "beautifying" software to make photos "look nicer" -- and it does a great job at this, but it may also be lacking in certain areas. I'd be interested if Photoshop can deal with inverse length scales such as on diffraction patterns, or if it can calibrate the intensity in values such as non linear length scales or other values. In the case Michael mentions above, for example, one should be able to change the look-up table to optimize the display without changing the underlying data.
Don't misunderstand me, please. I think Photoshop is a great product for what it does, but for heavy duty data reduction and quantitative analysis another software may be a better choice (Sorry if this sounds like a "plug" for ours, there are many others available as well).
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: michael shaffer [mailto:epmalab-at-darkwing.uoregon.edu] Sent: Tuesday, October 24, 2000 8:32 AM To: Microscopy-at-sparc5.microscopy.com
Bill Miller writes ...
} ... I said PhotoShop, and was asked how he would use it. } I told him the standard things - contrast } enhancement, color matching across different media, } annotation, resizing, etc. ... } ... } how else is PhotoShop being used - anything fancy to } justify the cost?
We use PS5 here for annotation and presentation of spacial elemental mapping (elemental x-ray maps), and average atomic number maps (backscatter electron images). There isn't better software for presenting this type of data in a "perceptually" uniform way. For example, if you have a grayscale elemental map which represents SiO2 from 0% to 100%, with most softwares and typical monitors the change from 20% to 30% isn't perceived the same as 70% to 80%, if perceived at all. Opening and converting such an image into a Photoshop gamma=2.2 working space makes this gradation perceptually uniform, and much better for presentation. The caveat here is the "conversion" when you do this ... that is, PS makes the simple opening of the file such an innocent process, the user may not notice his/her data has been modified. What was once a pixel value of '128' is now '183'!! The data is now useless for quantitative analysis. Make it a practice to keep (possibly even write-protect) your original image files, and put your PS projects in a separate directory.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
At last a chance to use some ancient knowledge to help out.
For Kodak products, there was a convention that said anything with a number as a name was a public domain type formula. So if you go into some old Kodak books you will find the formulas for things like D-19, D-11, D-76 etc.
Products that used a name for a name, like Dektol, were proprietary formulas, not generally available.
I probably have the formulas for most of the standard type numbered products. Let me know if you need one.
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
Knock off the political mumbo-jumbo. It is surely inappropriate here.
Nestor can you filter this stuff? -----Original Message----- } From: William R. Oliver {oliver-at-cpt.afip.org} To: Massimo Catalano {massimo.catalano-at-ime.le.cnr.it} Cc: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}
I think De Vere 504 is a good one. Depending on your need and the type of paper you use, you can choose different enlarger heads such as Ilford Ilfospeed Multigrde enlarger head.
Regards,
Kun Li
Systems on Silicon Manufacturing Company Pte Ltd FA Section --
On Tue, 24 Oct 2000 09:03:27 Jinguo Wang wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Get your FREE Email at http://www.mailcityasia.com
I have read that some dilution of Kodak HC110 made a good substitute for D 19. A call to Kodak's help line 800 242 2424 should get an answer. HC110 keeps for ever and can be replenished.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger
} From: "Geoff McAuliffe" {mcauliff-at-UMDNJ.EDU} } } -Hi All, } } I am looking for a good replacement for D19b developer (for nuclear emulsion plates, as used in our X-ray radiography and topography images, no longer available). We are using Suprol at the moment, but would like something as good as the old stuff. Anyone have any ideas? } } } } Richard } } } } ============================================================== } } Richard Beanland, } } Structural Analysis Lab, } } Caswell Technology, } } Caswell, } } Towcester, } } Northants NN12 8EQ } } } } e-mail richard.beanland-at-marconi.com } } Tel. +44 1327 356363 } } Fax. +44 1327 356398 } } } } How about mixing your own from scratch? I will see if I can dig up the formula tomorrow (Wed). } } Geoff } -- } ********************************************** } Geoff McAuliffe, Ph.D. } Neuroscience and Cell Biology } Robert Wood Johnson Medical School } 675 Hoes Lane, Piscataway, NJ 08854 } voice: (732)-235-4583; fax: -4029 } mcauliff-at-umdnj.edu } ********************************************** } } }
Here is a formulae for Developer D19b I have in an old file.
Metol 2.2 Hydroquinone 8.8 Sodium sulphite(anhydrous) 72 Sodium carbonate(anhydrous) 48 Potassium bromide 4 Water to 1 L.
D-19b was generally regarded as a high contrast developer for X-ray and industrial photography. Used undiluted at 68F, the average time for tank development is five minutes. This is a well balanced developer of good keeping properties.
D19b formula (quoted as a "manufacturer's formula" in British Journal Photographic almanac for 1955)
metol 2.2g hydroquinone 8.8g sodium sulphite cryst. 144g sodium carbonate cryst. 130g potassium bromide 4g water to 1litre
Chris
Date sent: Tue, 24 Oct 2000 17:37:32 +0000 (GMT) } From: Richard Beanland +44 1327 356363 {richard.beanland-at-marconi.com}
If it's ordinary D-19 developer there are numerous sources for this formula published. There's also one for a replenisher called D-19R (replenisher). However, I've not seen a D-19b. I've used D-19 for TEM plates (Kodak SO-163 and 4489) for years and it's readily available in those familiar yellow packages, at least here in the states. The formula (from Kodak's publication J-1):
Water, about 50 degrees C 500ml
Kodak ELON Developing agent (aka Metol or p-methylaminophenol sulfate) 1.0 gram Kodak Sodium sulfite (anhydrous) 75.0 grams Kodak Hydroquinone 9.0 grams Kodak Sodium Carbonate (monohydrate) 30.0 grams Kodak Potassium Bromide (anhydrous) 5.0 grams Cold water to make 1 liter.
At a state-side price, for working solution, of about $5.00 for 3 gallons I buy those little yellow packages. In formulating related photographic solutions, I've found no difference in using equivalent bulk chemicals of different manufacture. As I recall the above formula is also in the Ilford manual (probably sans the Kodak trade names).
See what develops,
John
No financial interest in the Eastman-Kodak Company
Jon Krupp wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } At last a chance to use some ancient knowledge to help out. } } For Kodak products, there was a convention that said anything with a number } as a name was a public domain type formula. So if you go into some old } Kodak books you will find the formulas for things like D-19, D-11, D-76 } etc. } } Products that used a name for a name, like Dektol, were proprietary } formulas, not generally available. } } I probably have the formulas for most of the standard type numbered } products. Let me know if you need one. } } Jonathan Krupp } Microscopy & Imaging Lab } University of California } Santa Cruz, CA 95064 } (831) 459-2477 } jmkrupp-at-cats.ucsc.edu
My posting for the D-19 formula was wrong. My message was for D-11 which I've used for higher contrast work. Looks like the correct D-19b formula has been posted. It's different from regular D-19.
D.M. de Leeuw et al. Physica C 158 (1989) 391 prepared air and moisture sensitive samples for TEM by crushing them in a mortar under decanol and putting a droplet of the suspension onto a grid. Decanol has low evaporation rate and thus protects the sample. However, it evaporates inside microscope in air-lock but probably also in the column. I would like to know if the procedure is save for modern UHV microscopes and/or there are other procedures that could be safely used for similar air sensitive samples.
Leszek Kepinski
Institute of Low Temperature and Structure Research, Polish Academy of Sciences, P.O.Box 1410, 50-950 Wroclaw, Poland
} Knock off the political mumbo-jumbo. It is surely inappropriate here. } } Nestor can you filter this stuff?
Sheesh. Gals and guys, it was a *joke,* see. You know, there was this big flap about a certain politician inserting subliminal messages into things (even though he couldn't *pronounce* the word), and I was trying to leaven my reply to Dr. Catalano with a little humor. You know -- inserting inappropriate short statements sotto voce. Ever see Kevin Nealon's schtick on "Subliminal Man"?
As described by Dr. Gary Radford in "Subliminal Persuasion in the Mass Media" http://alpha.fdu.edu/~gradford/subliminal.html:
Kevin Nealon, a comic on Saturday Night Live, portrays a character called "Subliminal Man." Subliminal Man persuades people to do his bidding by uttering "hidden," but in fact wholly audible messages under his breath. In a commercial for Miller Lite, he says: "What they won't do in commercials these days [buy Miller Lite] - I mean, they resort to all sorts of tactics [brainwash]. Now take Miller Lite [six pack], it has a great pilsner taste [gonna love it]." At the end of the ad, Subliminal Man propositions a beautiful blonde: "Hi [your place], would you like to join me for a Lite [your treat]? It's on me." She coos back: "OK, but it's my treat."
And, you see, it *had* to be Bush, because he was the one in the news about it.
Sorry, I had made the obviously silly assumption that this was a pretty transparent schtick which merely attempted to sugar-coat the real message, which was that frank poltical discussions *were* inappropriate. You chose to ignore *that* message and instead only read the bullet insertions between the paragraphs.
I would make some correllations between stiffness, lack of sense of humor and likely candidate support, but I will leave that be for now.
Henceforth I promise never to use humor in this group and *only* reply in terms of self-righteous outrage and stern condemnation.
I hope this isn't an inappropriate question, but we have some quick decisions to make.
I've queried the list a couple times before about peoples' experiences with non-OEM equipment insurance contracts, but now I need to ask a more specific question. I would be very interested in hearing from people who have experience with CIC Corp and/or Kemper Cost Management, particularly with electron microscopes and ancillary equipment. Both positive and negative comments are very welcome.
Please (pretty please?) reply to me directly and not to the listserver, especially if you have negative comments. I think Nestor frowns on public company bashing and I don't blame him.
Thanks much.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
Hi, I am a graduate student and I need some help trying to figure out a quantitative way to evaluate my samples before running my experiments with them. I am using a technique we call nanosphere lithography by which we deposit polystyrene spheres onto a substrate. We want a single layer of hexagonally packed spheres. My spheres are 400nm across which means the spaces between them are just under 100nm. We get this packing in some areas and can see a nice, dark blue color. However, just as in crystal growth we also see grain boundries and other defects where we may have no spheres or multiple layers of spheres. My substrates are 12 - 15 mm square ITO coated glass. AFM is not a good solution because it takes entirely too long to look at an entire sample and even then, I only have my observations to say which area is the best. Someone mentioned stereology to me but I am unsure of what that technique entails and what information I can gain from it. I am looking for something that will be able to look at the entire sample and eventually give me some sort of number such as the % that is perfect or even the % that is defective. We have an 80X objective but that isn't enough resolution to see the tiny spheres in enough detail. However, I have access to some other facilities on campus but need to know what to look for. Does anyone have any suggestions? Thanks for your time and any comments are appreciated.
I have been very happy with my Durst Point Source enlarger with a varicontrol illuminator. However before you invest over 10K in such a device, you really should investigate the possibilities of a CCD camera upgrade for your scope. I was at the Microscopy and Microanalysis 2000 meeting this past summer in Philadelphia, and based on the many different vendors of such devices, it is obvious that the days of film are not unlimited. While a CCD camera and its related computer needs are expensive up front, the product they produce is very manuscript and grant application form friendly. For labs with space restrictions, a computer work station certainly takes up less square footage of floor space compared to a dark room and does not have to expose the operator and the environment to potentially hazardous chemistry that is associated with conventional dark room operations. The down side of CCD imaging is the upfront cost and not quite as high resolution compared to film. Tim
Timothy G. Schneider Director of Electron Microscopy Department of Pathology Room 229 Jefferson Hall Thomas Jefferson University 1020 Locust St. Philadelphia Pa. 19107 215-503-4798 work 610-613-8170 cellular timothy.schneider-at-mail.tju.edu
Speaking of which I have a self-righteous stage from a condemned stem free to a good home. Unfortunately it's contaminated with subliminable materials, but could be cleaned with a solution of vacuous politicians in HF. Replies by bush telegraph only please Chris } } Henceforth I promise never to use humor in this group and *only* } reply in terms of self-righteous outrage and stern condemnation. } } } billo } }
========================================== Dr. Chris Jeffree University of Edinburgh BIOSEM - Biological Sciences Electron Microscope Facility Institute of Cell and Molecular Biology Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 (0) 131 650 5345 FAX. #44 (0) 131 650 6563 Mobile 07710 585 401 email c.jeffree-at-ed.ac.uk =========================================
We are presently purchasing several student polarizing microscopes, plus one which can be used with a digital camera for classroom presentation. We are initially considering the Coolpix 950/990 with its appropriate adapter on a 1X C-mount. During a demo yesterday, we were quite impressed with a Coolpix's NTSC output ... color as good as we've seen with more expensive cameras. However, we were a bit disappointed when we tried to grab a digital image. The camera would vignette much more than the video display would indicate (there was actually no vignetting with the NTSC out) ... although the color is quite accurate. But before I point you at examples, let me first explain we tried this demo with my personal CP800, believing we should get performance very similar to the CP950. The vignetting problem may be due to a feature setting available on the 950 I am not aware of. Point your browser at the URLs below ... the 1st is full image, so you can inspect the detail ... the 2nd will show you the vignetting.
As a starter, you need to get your hands on John Russ' Image Analysis Handbook to find out what stereology or Quantitative Microscopy can do for you. There are other books that you can find on the subject, but they are cited in his book.
I would suspect that you might be able to process your using an FFT to filter the periodic structure so that the grain boundaries are enhanced and then further enhance the grain boundaries so that you can quantify them. There are a couple of standard quantitative measurements that you can make at this point to characterize your microstructure. You can do the measurements by hand, but the computer is much easier. John Russ has a series of Plug-ins for doing these measurements and image enhancements in Photoshop and NIH image that are a companion to his book.
There are also a number of other commercial products out there that might be able to help you with this, but they would be more expensive. NIH Image is an alternative software package that is free and John's plug-ins also work there.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
} -----Original Message----- } From: Anjeanette Ormonde [mailto:ormonde-at-chem.nwu.edu] } Sent: Wednesday, October 25, 2000 10:27 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Suggestions needed/stereoscopy? } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht} ml } } } -------------------------------------------------------------- } ---------. } } } Hi, } I am a graduate student and I need some help trying to } figure out } a quantitative way to evaluate my samples before running my } experiments } with them. I am using a technique we call nanosphere } lithography by which } we deposit polystyrene spheres onto a substrate. We want a } single layer } of hexagonally packed spheres. My spheres are 400nm across } which means } the spaces between them are just under 100nm. We get this } packing in some } areas and can see a nice, dark blue color. However, just as } in crystal } growth we also see grain boundries and other defects where we } may have no } spheres or multiple layers of spheres. My substrates are 12 - 15 mm } square ITO coated glass. AFM is not a good solution because it takes } entirely too long to look at an entire sample and even then, } I only have } my observations to say which area is the best. Someone mentioned } stereology to me but I am unsure of what that technique } entails and what } information I can gain from it. I am looking for something } that will be } able to look at the entire sample and eventually give me some sort of } number such as the % that is perfect or even the % that is } defective. We } have an 80X objective but that isn't enough resolution to see the tiny } spheres in enough detail. However, I have access to some } other facilities } on campus but need to know what to look for. Does anyone have any } suggestions? Thanks for your time and any comments are appreciated. } } Angie Ormonde } } }
Does anybody know a good way to clean a dirty Faraday cage? (I know, I shouldn't have let it get dirty in the first place....:-() I'm thinking sonification in soapy water with lots of rinsing afterwards, but I wonder if there are any drawbacks to this. I'm hesitant to use solvents because I don't know what "glue" was used to attach the little aperture on top, and it was way too expensive to screw up that way. I tried a puff of air from one of those little rubber squeeze-bulb thingies (whatever the hell they're called) but that just didn't do the trick. I don't really know what the contamination is but it's partially transparent in a 20KeV beam. Could be anything but metal, I guess.....
F.C. Thomas MicroAnalysis Facility Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada B2Y 4A2
The enlargers made by Durst, Beseler and Omega have always been considered among the best. A condenser system for the light source and a point light source will give you the sharpest image. I have had several Durst enlargers because of their nice point light source. It helps to buy the best lens available. Schneider lenses have a good reputation (Apo Rodagon etc). The enlarger usually must be one designed for use with up to 4" X 5" negatives in order to acommodate the EM negatives. In the past I purchased special negative carrierers from a small company on the east coast named Carlwen. I recommend getting assistance from your local professional photography dealer. Larry D. Ackerman Lily & Yuh Nung Jan Laboratories Howard Hughes Medical Institute UCSF, Box 0725, Rm U226 533 Parnassus Ave. San Francisco, CA 94143
A huge thankyou to the many of you who responded to my query about D19b. Several people gave me the recipe, and one kind person even had a can of the stuff they were willing to send to me! I am a happy man.
e-mail richard.beanland-at-marconi.com Tel. +44 1327 356363 Fax. +44 1327 356398 ============================================================== "The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone. Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."
I assume that you mean oxygen sensitive samples when you say air sensitive.
In that event you might try crushing the specimen in a glove box/bag purged with a continual flow of dry nitrogen gas. You can mount the specimen on the TEM grid and then the grid in the TEM holder all within the glove bag. Exposure to oxygen and atmospheric water vapor would be limited to the brief interval between removing the holder from the bag and inserting it into the microscope column. In fact, I have seen people do all this in a nitrogen purged plastic bag that also encloses the TEM's airlock to avoid oxygen exposure during transfer to the airlock.
This may be worth a try if you are concerned about Decanol evaporation in the TEM column.
} D.M. de Leeuw et al. Physica C 158 (1989) 391 prepared air and moisture
} sensitive samples for TEM by crushing them in a mortar under decanol and } putting a droplet of the suspension onto a grid. Decanol has low evaporation } rate and thus protects the sample. However, it evaporates inside microscope } in air-lock but probably also in the column. I would like to know if the } procedure is save for modern UHV microscopes and/or there are other } procedures that could be safely used for similar air sensitive samples.
} } Leszek Kepinski
-- _____________________________________________________ Tyrone L. Daulton Director: Marine Geosciences - Electron Microscopy Center Physicist and Senior Electron Microscopist
Naval Research Laboratory Marine Geosciences Division (Code 7400) Stennis Space Center, MS 39529-5004
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I am a graduate student and I need some help trying to figure out a quantitative way to evaluate my samples before running my experiments with them. I am using a technique we call nanosphere lithography by which we deposit polystyrene spheres onto a substrate. We want a single layer of hexagonally packed spheres. My spheres are 400nm across which means the spaces between them are just under 100nm. We get this packing in some areas and can see a nice, dark blue color. However, just as in crystal growth we also see grain boundries and other defects where we may have no spheres or multiple layers of spheres. My substrates are 12 - 15 mm square ITO coated glass. AFM is not a good solution because it takes entirely too long to look at an entire sample and even then, I only have my observations to say which area is the best. Someone mentioned stereology to me but I am unsure of what that technique entails and what information I can gain from it. I am looking for something that will be able to look at the entire sample and eventually give me some sort of number such as the % that is perfect or even the % that is defective. We have an 80X objective but that isn't enough resolution to see the tiny spheres in enough detail. However, I have access to some other facilities on campus but need to know what to look for. Does anyone have any suggestions? Thanks for your time and any comments are appreciated.
Dear Angie, Here are a couple of suggestions. I don't know how practical they are, but they may suggest more practical possibilities. To get the average number of spheres per unit area, you could try to measure the fraction of incident light absorbed. Illuminate the specimen with a wavelength of light which is at the absorption max, and either collect all the light which is not absorbed (including scattered light), or measure the energy absorbed with a calorimeter. The latter does not require collecting the scattered light, but may have other problems, such as accounting for heat re-radiated. You would need to determine the absorption of one sphere by calculating it or by measurement of a dilute suspension of spheres in a medium which does not absorb light at the wavelength selected and which has the same index of refraction as the spheres. To get a measurement of the packing, you could try to measure the diffraction of a parallel beam of light incident on the specimen. This time, the spheres should not absorb at the wavelength selected. You could calculate the expected intensity and compare this with what is observed. The spread of the diffraction spots will give a measurement of the regularity of the crystal, and the appearance of two or more distinct hexagonal patterns will tell you that there is more than one patch being illuminated, and that the patches are about the same size as the illuminating spot--assuming that the spot is not on the boundary between two large patches (which is easily determined). The appearance of rings or circles of many spots will indicate that many small patches are being illuminated, and other possible patterns can be interpreted. As I said, there are no guarrantees that either of these measurements are useful, but good luck. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
Hello everyone, I was wondering if anyone had suggestions on a protocol for the fixation of either newt or squid eye to be viewed in the SEM. I will be viewing a crossection of the eye. Would it be best to fix after cutting the eye, or try to perform a frozen fracture after fixation and dehydration. Any suggestions will be appreciated. Thanks, Thomas
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Sorry I don't know the answer to your quaestion, but I just can't stop myself from asking:-
- How do you know it's dirty? - How would you have prevented it from getting dirty? - Does it matter, anyway?
cheers
rtch
} } Does anybody know a good way to clean a dirty Faraday cage? (I } know, I } shouldn't have let it get dirty in the first place....:-() I'm } thinking sonification in soapy water with lots of rinsing } afterwards, but I wonder if there are any drawbacks to this. I'm } hesitant to use solvents because I don't know what "glue" was used } to attach the little aperture on top, and it was way too expensive } to screw up that way. I tried a puff of air from one of those little } rubber squeeze-bulb thingies (whatever the hell they're called) but } that just didn't do the trick. } I don't really know what the contamination is but it's partially } transparent in a 20KeV beam. Could be anything but metal, I } guess.....
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
} To: John Heckman {heckman-at-pilot.msu.edu} } From: Gary Gaugler {gary-at-gaugler.com} } Subject: D-19 } } Kodak still makes D-19. Part numbers are: } } 1464593 1 gallon } 1946045 5 gallons } } gary g.
Have you tried SEM? The size should be easily resolvable in any SEM. You may have to coat your sample to avoid charging.
Then take your images and run some FFTs on them. That should give you information about packing and also faults in the packing. For a quantitative analysis you probably need to design some filters in Fourierspace, and do a back transform, then analyze those images.
There are a bunch of books out there that explain things in detail. John Russ' book comes to mind.
Good luck.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Anjeanette Ormonde [mailto:ormonde-at-chem.nwu.edu] Sent: Wednesday, October 25, 2000 8:27 AM To: Microscopy-at-sparc5.microscopy.com
Hi, I am a graduate student and I need some help trying to figure out a quantitative way to evaluate my samples before running my experiments with them. I am using a technique we call nanosphere lithography by which we deposit polystyrene spheres onto a substrate. We want a single layer of hexagonally packed spheres. My spheres are 400nm across which means the spaces between them are just under 100nm. We get this packing in some areas and can see a nice, dark blue color. However, just as in crystal growth we also see grain boundries and other defects where we may have no spheres or multiple layers of spheres. My substrates are 12 - 15 mm square ITO coated glass. AFM is not a good solution because it takes entirely too long to look at an entire sample and even then, I only have my observations to say which area is the best. Someone mentioned stereology to me but I am unsure of what that technique entails and what information I can gain from it. I am looking for something that will be able to look at the entire sample and eventually give me some sort of number such as the % that is perfect or even the % that is defective. We have an 80X objective but that isn't enough resolution to see the tiny spheres in enough detail. However, I have access to some other facilities on campus but need to know what to look for. Does anyone have any suggestions? Thanks for your time and any comments are appreciated.
I am not familiar with the CoolPix cameras and the NTSC out function. Do they have that available right off the camera? Is your video monitor overscanning? Many do so that you do not see the entire height or width of the image. If so, the vignetting would not appear too bad until you grabbed the image and saw it all. Some monitors include an underscan switch so you can see the whole image.
I suppose someone (Barbara Foster maybe) is going to weigh in on the subject of vignetting in general. It seems that there have been several discussions on this a few months back. I thought the basic problem was using a transfer lens of too low a power for the camera. I have the opposite problem with our Pixera (or any other 1/3" camera). We are using a 0.5x transfer lens. A 0.33x would be best to match the field of view and the picture, but they were quite a bit more than the 0.5x lenses.
Warren S.
At 07:43 AM 10/25/2000 -0700, you wrote:
} We are presently purchasing several student polarizing } microscopes, plus one which can be used with a digital camera for } classroom presentation. We are initially considering the Coolpix } 950/990 with its appropriate adapter on a 1X C-mount. } During a demo yesterday, we were quite impressed with a Coolpix's } NTSC output ... color as good as we've seen with more expensive } cameras. However, we were a bit disappointed when we tried to grab a } digital image. The camera would vignette much more than the video } display would indicate (there was actually no vignetting with the NTSC } out) ... although the color is quite accurate. But before I point you } at examples, let me first explain we tried this demo with my personal } CP800, believing we should get performance very similar to the CP950. } The vignetting problem may be due to a feature setting available on } the 950 I am not aware of. } Point your browser at the URLs below ... the 1st is full image, so } you can inspect the detail ... the 2nd will show you the vignetting. } } http://darkwing.uoregon.edu/~mshaf/dogsci/x-biot.jpg } http://darkwing.uoregon.edu/~mshaf/dogsci/x-biot-sm.jpg } } cheerios, shAf :o) } } {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} } Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu } Geological Science's Electron Probe Facility - University of Oregon } http://epmalab.uoregon.edu/
We just received our Fuji FinePix Pro S1 camera. Beautiful instrument!!
I have not had luck interfacing it the a light microscope since the camera stubornly insists on having an autoNikkor lens attached to it. The usual microscope adapters cause the camera to be uncommunicative with the computer.
I KNOW that it can be interfaced with a LM and computer because I saw it in action at M&M2000.
So, does anyone know how to do this?
Several people are holding their breaths on this one.....
John B. -- #################################################################### John J. Bozzola, Ph.D. Professor & Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
I just had a demo yesterday of the 990 on a Leica Orthoplan II. The vignetting you showed was not present on our demo. Perhaps the zoom was set wrong or the wrong type of relay lens was used. I did see a slight amt of vignetting but it was minor--nothing like what you showed.
We are definitely going to buy the 990 along with relay lens, wired remote control and AC adapter. We got a price of $1864.
I really liked the software too.
JB
-- #################################################################### John J. Bozzola, Ph.D. Professor & Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
I expect that Faraday cage is actually a F cup - since it has an aperture on top. The F cage attracts low energy electrons towards the scintillator and the cup is of course used to trap electrons and measure their current with a picoampmeter for analysis. We made our own when turning an SEM into a Probe about 20 years ago. Used were an aperture holder, with a bit of machined carbon rod at the end and this was capped with a 50um aperture. The carbon/aperture cup had to be insulated from the holder. A fine wire and the aperture were held in place with a bit of carbon dag. Worked well, though insertion was not nearly as convenient as currently used pneumatic F cups.
The relevance is that there is nothing "magical" about that device. The cup most only conduct through the wire and meter to ground, but the cup must be electrically conducting. I would not hesitate to remove the aperture, clean this properly and replace it. A couple of small spots of dag would hold it in place. Contamination could cause the insulating layer around the cup to conduct and this would make the cup's reading useless. If you can measure with a good meter any continuity between cup and holder itself, then you may need to replace the insulating layer, which I expect is a bit of plastic sleeving; you should be able to improvise something and that may be easier than cleaning that plastic. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Thursday, October 26, 2000 2:26 AM, Frank Thomas [SMTP:thomasf-at-AGC.BIO.NS.CA] wrote: } } Listers - } } Does anybody know a good way to clean a dirty Faraday cage? (I know, I } shouldn't have let it get dirty in the first place....:-() I'm thinking } sonification in soapy water with lots of rinsing afterwards, but I wonder } if there are any drawbacks to this. I'm hesitant to use solvents because I } don't know what "glue" was used to attach the little aperture on top, and } it was way too expensive to screw up that way. I tried a puff of air from } one of those little rubber squeeze-bulb thingies (whatever the hell they're } called) but that just didn't do the trick. } I don't really know what the contamination is but it's partially } transparent in a 20KeV beam. Could be anything but metal, I guess..... } } F.C. Thomas } MicroAnalysis Facility } Bedford Institute of Oceanography } Dartmouth, Nova Scotia } Canada } Geological Survey of Canada (Atlantic) } Bedford Institute of Oceanography } Dartmouth, Nova Scotia } Canada B2Y 4A2
In a message dated 10/24/00 6:05:02 PM, mb-at-Soft-Imaging.com writes:
} I don't want to put down Photoshop, especially since we are producing a } competing product, but I think this remark points to one problem that many } "photo" applications have -- they do not treat the intensity as scientific } data. I may be mistaken, but I think Photoshop is conceptually a } "beautifying" software to make photos "look nicer" -- and it does a great } job at this, but it may also be lacking in certain areas.
Michael is partly right - Photoshop does not include native capabilities for calibration of intensity values for optical density, or the ability to directly measure hue in color images, or to calibrate spatial distances in microns, etc. It also has very limited image measurement capabilities. But it has many advantages (particularly ease of use, consistency on various computer platforms, a huge base of users so that few bugs survive, etc.) and all of the other features that other responders to the original question have pointed out, plus it is relatively inexpensive (particularly if you can take advantage of the academic price). Many microscopists already have it, as well. And the shortcomings are easily filled in using plug-ins. Those are the reasons that my son and I decided to use it as the platform for writing plug-ins to do scientific imaging. With the layers and actions capability in the most recent versions (5.0 and 6.0), and the Tool Kit or Fovea plug-in package (no Nestor, I won't make any further plug for the product - others have already mentioned it anyway), the capabilities for scientific quality image processing actually exceed those in many of the costly "commercial" packages.
I had a similar problem on my metallurgical microscope when I first installed a camera to it, at 25X and 50X
it would vignette but at higher magnifications it wouldn't . Kodak suggested a higher C-mount magnification and
that took care of it.
This info is from the Kodak site:
"3.What is a C-Mount and why does it need to be 1.0X? To accommodate the need for photo documentation, many microscopes feature an additional photo port or tube that delivers an optical path to a camera. It is the C-Mount that adapts the microscope to the KODAK MDS Universal Optical Adapter and MDS 290 digital camera. C-Mounts of less than 1.0X magnification may cause images to vignette (darkening around the edges of the image), and are therefore not recommended. "
I'm one of the breath holders, as I'm currently waiting for my microscope adapter to arrive. Got the camera and other goodies, but the adapter is on back order. As I read the book, it seems you can't use all the automatic bells and whistles unless you have a Nikon type D lens. Perhaps you need to switch to aperture priority or shutter priority mode? Anyone else have any ideas?
David Bell Scientist Electron Microscopy Lab Millipore Corporation 80 Ashby Road Bedford, MA 01730 (781) 533-2108
"John J. Bozzola" To: microscopy-at-sparc5.microscopy.com {bozzola-at-siu. cc: edu} Subject: Fuji S1 digital cams on LMs
10/25/00 06:28 PM
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
We just received our Fuji FinePix Pro S1 camera. Beautiful instrument!!
I have not had luck interfacing it the a light microscope since the camera stubornly insists on having an autoNikkor lens attached to it. The usual microscope adapters cause the camera to be uncommunicative with the computer.
I KNOW that it can be interfaced with a LM and computer because I saw it in action at M&M2000.
So, does anyone know how to do this?
Several people are holding their breaths on this one.....
John B. -- #################################################################### John J. Bozzola, Ph.D. Professor & Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
At 07:43 AM 10/25/00 -0700, michael shaffer wrote: } However, we were a bit disappointed when we tried to grab a } digital image. The camera would vignette much more than the video } display would indicate (there was actually no vignetting with the NTSC } out) ... although the color is quite accurate.
In your second image, in the lower right corner for example, I can see what I'd call true vignette, the sharp edge of the lens tube. I'd guess you do see that small dark area in the NTSC-out view. The rest of the ring of darkness could be the drop-off in the light intensity of the microscope's output, and perhaps an automatic exposure only properly exposed the center of the view. Were you using manual or automatic exposure settings? Did you try both methods? Where was the zoom set?
I recently purchased an Olympus C-2500L. This is a true SLR digital camera, roughly $1,000. I had high hopes of using it in an afocal coupling manner with my Ortholux and a Dobsonian telescope. In other words, I just wanted to point the camera into the eyepiece and get a good view. There are also ~$150 adapters that will hold the camera in place in these situations, like http://www.photosolve.com/xtendascope.asp .
I was very disappointed to learn that I should've bought a less expensive camera non-SLR for this purpose - although I've heard the Coolpix 8xx/9xx are suitable and otherwise nice, too. Those with non-SLR lenses are much better at this sort of afocal coupling. With the C-2500L, the front lens element doesn't move when I zoom/tele. I can't avoid vignette-ing at all.
A 43 mm Plossl eyepiece might help on the telescope, and I haven't had time to figure out which sort of microscope eyepiece might help. I have other archived e-mails from a few experts that I'd be glad to forward to anyone interested.
Good morning fellow listers, we have a patron that we are helping find a set of RCA EMU3-F manuals and prints. any info leading to that would be great. also if I could have the info on the folks that redo the florcesent screen i will pass that along too ... thanks Ed Sharpe archivist for SMECC
We have a home-grown program that we wrote for the purpose of translating batches of files into 8-bit TIFF. We then use other utilities to convert those TIFF files to JPEG.
I use it only occasionally, and it may still have some bugs in it. But let me check on its status and I can probably get it to you with some instructions.
At 09:38 PM 10/24/2000 +0200, you wrote:
} Does anyone know of software that will do a batch conversion of the } .IM_ image files captured using Link ISIS v. 3 Autobeam, to a standard } format (eg. pcx, jpg, etc)? } } Fiona } ____________________ } Dr Fiona Graham } EM Unit, } University of Natal, Durban } South Africa
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
I just got off the phone with the Fuji representative (thank you Gary) who worked with me to solve the interface problem with the Fuji S1 camera. Turns out that the software had to be set up properly. Now the system works without a hitch. The images are FANTASTIC.
We had originally purchased the camera for copystand, macro photography but I suspect that the S1 will be spending most of its life on the light microscope from this point on. It is supposed to work even on fluorescent images -- so I am looking forward to checking that out too.
Thanks also to Matt Irwin from ElectroImage who called and also helped point me in the right direction.
This camera is really wonderful!!!!
JB -- #################################################################### John J. Bozzola, Ph.D. Professor & Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
I have a Zeiss EM 9S 2, vintage 1975, which I would like to keep running for as long as possible. Parts are very difficult to obtain. If any of you are considering discarding one of these instruments, please get in touch with me. I'd be happy to take it off your hands. Thanks.
Good morning everyone, I' m looking for specimen preparation to Low-Vacuum SEM observation. I work with a Jeol, JSM-5600LV and I' d like to see lactic microorganisms and microalgae with that SEM. Does anyone Know "SEMpore" which is a registered mark of Jeol A. B. used for some authors to observe Cryptosporidium using a LV SEM? I' m hoping that someone can give me some advice to specimen preparation. Regards, Patrícia. _____________________________________________ Patrícia João Reis Escola Superior de Biotecnologia Rua Dr. António Bernardino de Almeida 4200 - 072 Porto Portugal Telef. +351 225580044 Fax. +351 22 5090351 E-mail: pmreis-at-morango.esb.ucp.pt
I am planning to use reflectance microscopy on live cells and I am looking for bathing solutions that can increase the reflectance from the cell membrane without harming the cells. Any suggestions ? Are there such products out there ? Thanks,
Isaac Bankman Johns Hopkins University Applied Physics Laboratory
FWIW: When IXRF images are exported to TIFF, the code allows a max array of 1024. Larger images are reduced to that on export. Sometimes a high resolution / low mag image is useful to have. I have had limited success using photoshop to read the native files. I change the file type to .raw then open w/photoshop. PShop will try to "guess" the header size. Seems to work well with 4Kx4K arrays, but fails with 2K square. Since I'm after an image allowing me to digitally zoom in and roam around, this is not sooo bad.
Woody White McDermott Technology, Inc Lynchburg Research Center
Me: http://home.att.net/~woody.white
{ {SNIP} } } } We have a home-grown program that we wrote for the purpose of } translating } batches of files into 8-bit TIFF. We then use other utilities } to convert } those TIFF files to JPEG.
I have very good luck cleaning things that go into electron optical instruments using Tiles Soap Scum Remover. This is a mixture of high powered detergents combined with a organic solvent (diethylene glycol butyl ether). I just scrub parts with this thoroughly, or treat them with it ultrasonically, then rinse them thoroughly with hot running water, and finish by rinsing with reagent grade isopropyl alcohol and blowing off the residual alcohol with a duster. I've been able to remove pump oils, machining oils, and even silicone grease in this way, and have had no problems when parts so cleaned are later put in a high vacuum system.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
I thought I'd get back to you all and let you know the problem of vignetting was not a question of "zoom" (although you cannot zoom out and not see true vignetting because of the tube). The problem was apparently my CP800 and its aperture adjustment for proper exposure.
Thanx to those who suggested I turn off the "flash" which sets a different aperture priority, ... and fixing the focus at infinity. I can now take photos similar to what was posted previously and not see vignetting ... 'cept very slightly which suggests I ought to increase the zoom only slightly, but might be corrected properly with the larger CP990's CCD.
However, I still experience similar vignetting for very bright images .. the CP800 will still close down the aperture for compensation. However, this occurs (for example) if I take picture with crossed polars, and then want the same image without crossed polars ... but I could fix this with a neutral density filter.
I haven't yet had a chance to play with a CP950 or 990, but presumably these "better" cameras come with more features ... possibly a "aperture priority" mode which would lock-out the problem.
I cannot yet post images until I've had time to download to my home computer ... possibly next week.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
From root Fri Oct 27 18:58:46 2000 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id OAA10026 for dist-Microscopy; Fri, 27 Oct 2000 14:48:54 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id OAA10022 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 27 Oct 2000 14:42:44 -0500 (CDT) Received: from srvr20.engin.umich.edu ([141.213.75.22]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id OAA10013 for {microscopy-at-sparc5.microscopy.com} ; Fri, 27 Oct 2000 14:42:12 -0500 (CDT) Received: from [141.212.131.74] (dow2146amac74.engin.umich.edu [141.212.131.74]) by srvr20.engin.umich.edu (8.9.3/8.9.1) with ESMTP id PAA03915 for {microscopy-at-msa.microscopy.com} ; Fri, 27 Oct 2000 15:38:18 -0400 (EDT) X-Sender: bigelow-at-srvr5.engin.umich.edu Message-Id: {v03007803b61f7b42c740-at-[141.212.131.74]} Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
I have very good luck cleaning things that go into electron optical instruments using Tiles Soap Scum Remover. This is a mixture of high powered detergents combined with a organic solvent (diethylene glycol butyl ether). I just scrub parts with this thoroughly, or treat them with it ultrasonically, then rinse them thoroughly with hot running water, and finish by rinsing with reagent grade isopropyl alcohol and blowing off the residual alcohol with a duster. I've been able to remove pump oils, machining oils, and even silicone grease in this way, and have had no problems when parts so cleaned are later put in a high vacuum system.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
The next meeting of the Minnesota Microscopy Society will be on Thursday, November 9, 2000
The theme of the meetng is "Everything you wanted to know about SPM but were afraid to ask"; A talk to be given by Chuck Mooney, SPM Assistant Product Manager, JEOL USA, Inc.
Abstract: The first scanning probe microscope (SPM), the scanning tunneling microscope, was introduced to the world in 1981 as a frightfully complex laboratory experiment where conductors could be probed at the atomic scale. Five years later, the Nobel Prize in Physics was awarded to its inventors, even as the basic technique was expanded to include probing insulators with the invention of the atomic force microscope (AFM). Since that time, many new methods have been added to the general field of scanning probe microscopy that allow for the probing of all manner of samples and forces. Recent breakthroughs have allowed the measurement of the strength of bonds between atomic species as well as the potential for 3-D sub-surface imaging and spectroscopy of atomic species near the sample surface. Many research laboratories have added scanning probe microscopy techniques to their analytical arsenals aided by the introduction of commercially available instruments. Unfortunately, the strengths and weaknesses of SPM remain a mystery to many who could benefit from a better understanding of both the techniques that are routine and those that are difficult, the relative levels of information that can be extracted from those techniques, and the types of samples that are suitable for most SPMs. In the time allowed, an introduction to the various SPM techniques will be given, applications examples shown, as well as providing insight into the future of probe microscopy.
Location: Medtronic, Inc., 6700 Shingle Creek Parkway, Brooklyn Center
Dinner: New King Buffet, 5927 John Martin Drive, Brooklyn Center
The New King Buffet is a full menu Chinese buffet. Cost is $10.00 per person.
We will first meet at the New King Buffet for dinner. After dinner, we will reconvene at Medtronic for the business and technical meetings.
For reservations contact Mike Coscio by Friday, November 3: (763-514-1331, or mike.coscio-at-medtronic.com).
__________________ Stuart McKernan stuartm-at-tc.umn.edu Office:(612) 626-7594 IT Characterization Facility, University of Minnesota Desk:(612) 624-6009 100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368
I'd like more CPD advice again. I just had a very bad run on our new Pelco CPD2--stuff came out visibly wet! And the o ring on the door is already stretched out of shape. It's the last straw for my supervisor, we're sending it back. But now we would like to know what to get. Does anyone have a Tousimis Samdri-type drier? How is it? Any other *users* with recommendations who want to tell us why they like their CPDs? Mail from company reps, while not solicited, will be nonetheless noted.
Thanks for the help from those who wrote last time, and thanks in advance to those with responses.
From root Fri Oct 27 19:04:09 2000 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id OAA10063 for dist-Microscopy; Fri, 27 Oct 2000 14:57:44 -0500 (CDT) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id OAA10059 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 27 Oct 2000 14:51:16 -0500 (CDT) Received: from darkwing.uoregon.edu (darkwing.uoregon.edu [128.223.142.13]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id OAA10052 for {Microscopy-at-sparc5.microscopy.com} ; Fri, 27 Oct 2000 14:51:00 -0500 (CDT) Received: from mshaf (shaf.uoregon.edu [128.223.94.20]) by darkwing.uoregon.edu (8.10.1/8.10.1) with SMTP id e9RJYN907892 for {Microscopy-at-sparc5.microscopy.com} ; Fri, 27 Oct 2000 12:34:23 -0700 (PDT)
I thought I'd get back to you all and let you know the problem of vignetting was not a question of "zoom" (although you cannot zoom out and not see true vignetting because of the tube). The problem was apparently my CP800 and its aperture adjustment for proper exposure.
Thanx to those who suggested I turn off the "flash" which sets a different aperture priority, ... and fixing the focus at infinity. I can now take photos similar to what was posted previously and not see vignetting ... 'cept very slightly which suggests I ought to increase the zoom only slightly, but might be corrected properly with the larger CP990's CCD.
However, I still experience similar vignetting for very bright images .. the CP800 will still close down the aperture for compensation. However, this occurs (for example) if I take picture with crossed polars, and then want the same image without crossed polars ... but I could fix this with a neutral density filter.
I haven't yet had a chance to play with a CP950 or 990, but presumably these "better" cameras come with more features ... possibly a "aperture priority" mode which would lock-out the problem.
I cannot yet post images until I've had time to download to my home computer ... possibly next week.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
Al Coritz Sales & Service Manager RMC-Boeckeler Instruments 4650 S. Butterfield Dr. Tucson, AZ 85714 Voice: 520-745-0001 Cell: 520-465-3598 Fax: 520-745-0004 Email:Al-at-Boeckeler.com Website:RMCProducts.com
Before damning the Pelco, are you certain your methods are good? I do not know how experienced you are with CPD, so forgive me if you have thought of the following already. However, CPDs are very simple systems and most problems arise from operator errors, or failure to understand the basic principles
If the specimens are visibly wet then they are, presumably, wet either with the transition fluid or water - What fluid are you using? amyl acetate, acetone, ethanol, or something else? Can you smell it? Is the wetness of the specimen with the transition fluid or with water? If water, the specimens are not properly solvent-dehydrated. If transitional solvent, the sovent has not been removed by the CO2
The immersion in liquid CO2 should very quickly remove the transition fluid unless
1) the specimens were not fully immersed in liquid CO2, but only in gas - you must either use the dip-tube type of CO2 cylinder, or invert a non-dip type cylinder to obtain a supply of liquid CO2 to the CPD chamber. Remember that the dip-tube type must actually contain a liquid level - for some time after the available liquid is exhausted the cylinder will continue to supply gas, but you cannot do CPD with CO2 gas
2) insufficient liquid CO2 flushing took place, so some of the transition fluid remained in the chamber: flush for longer, or for short bursts with soak-time between
3) The specimens were very large, and insufficient CO2 rinsing time was allowed - see 2
4) The specimens were still wet with water: use more solvent-drying time with drier solvents
I think I am right in saying that the dehydration and transition fluid stages are where your problems should be sought, and that errors of method or hardware faults in or leading up to the actual critical point stage would not lead to wet specimens if the earlier procedures were correctly followed.
Chris
} Dear listers,
{color} {param} 0000,0000,0000 {/param} }
} I'd like more CPD advice again. I just had a very bad run on our new Pelco
} CPD2--stuff came out visibly wet! And the o ring on the door is
} already stretched out of shape. It's the last straw for my supervisor,
} we're sending it back. But now we would like to know what to get. Does
} anyone have a Tousimis Samdri-type drier? How is it? Any other *users*
} with recommendations who want to tell us why they like their CPDs? Mail
} from company reps, while not solicited, will be nonetheless noted.
}
} Thanks for the help from those who wrote last time, and thanks in advance
} to those with responses.
}
} Pauline Yu
} splene-at-pw.usda.gov
} Microscopist Technician
} USDA-ARS-WRRC
}
}
{nofill} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
Pauline, I agree with Chris Jeffree on two important points: 1. use bone-dry liquid carbon dioxide with a dip tube 2. use several steps of 100% ethanol (I recommend that all using our older Polaron E3000 use an unopened pint bottle of 100% ethanol in the last two ethanol steps. This is esp. important during the summer months when it is humid here) and would add the following tips --when dehydrating plant samples esp. vegetative or reproductive meristems, leave samples overnight in the third 100% ethanol and change again the next day before transferring to the CPD --when dehydrating drosophila or other whole insects, add several overnights in 100% ethanol --after filling the pressure chamber with the liquid carbon dioxide, check to see a meniscus, an indication that you have liquid carbon dioxide (for safe viewing, it's recommended that one do this on our system by directing a flashlight into the chamber while viewing the chamber via a mirror placed opposite to the chamber window) -- if the temperature of your chamber is higher than 19C, the carbon dioxide will fill the chamber and the pressure gauge will read 800psi but what you may have is vapor --use a flushing step just after filling with liquid carbon dioxide, i.e., with the inlet open, open the drain valve and monitor the drain line (at first, you'll see liquid (ethanol), a slush (ethanol/CO2) and then white powder (dry ice) -- many of our users place samples in the polypropylene specimen carriers which hold the ethanol when capped and we find it advantageous to use this step each time so that our subsequent exchanges are replacing the ethanol within tissues or organisms --any problems we had with gaskets were solved by purchasing those supplied by Energy Beam Sciences. Rosemary
Try staying simple. We have used two Polaron E3000 CP Dryers for at least 25 years and they have been very reliable. Some gaskets and seals have had to be changed but other than that we have had no problems. They do not have the automated features that some of the others have but they produce good results.
I think they are sold in the US by SPI, and perhaps other vendors too.
Good luck.
Rob
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa **
Right now Stormfront Studios is adding to its staff and looking for people to create Next Gen Games for the X-BOX and PS2. We're looking for an Entry Programmer, AI Programmer, Sr. Programmer, Lead Programmer, Development Director, Sr. 3D Max Artist, 3D Animator and/or Art Director. Interested or know of anyone who may be interested? Please let me know if you can help us out - we need your talent!
Thanks, Marta Daglow EMAIL: mdaglow-at-earthlink.net WEB: www.stormfront.com San Rafael, CA 94903
Dear colleagues, I would like to carry out experiment of irradiating an object with electrons in presence of gas media (about 1 torr) and intend to use CamScan4 SEM for this purpose. This device doesn't contain low vacuum accessories, so I hope to make them by myself. Could you tell me which are the tipical holes sizes in the differential pumping apertures, the distance between them and expected pressure increasing in diff. pump entrance. Thanks in advance. Eugeny Jikharev, Physics & Technology Institute of Russian Academy of Sciences, Moscow.
I am looking for some TEM negative film holders for Philips/FEI microscopes. These are holders with the format 3,25 x 4’’ and have two components. The holder itself and the insertion (for loading film and imaging plates). Placing an order in Germany it takes 6 months to get them – quite a long time. Is anybody out there who knows an other source to get the mentioned holders faster or has someone any used material available ?
Ruediger Kuenzler -- DIBIS Digital Biomedical Imaging Systems AG Gewerbestraße 11; D-75217 Birkenfeld Tel.: +49 (0)7082 940639 Fax : +49 (0)7082 940076 E-Mail: contact-at-dibis.de E-Mail: kuenzler-at-dibis.de Internet: http://www.dibis.de
Our laboratory is looking for an assistant to help prepare grants and publications. Below is the text of an announcement written for our HR department. In the past this position has been held by recent college graduates preparing applications for medical or graduate school or by trained research personnel interested in re-entering the workforce. -Brandon Poe, Ph.D.
===
We are looking for someone to fill a full- or part-time, temporary job, helping our neuroscience research laboratory to prepare reports on our research for publications and grant applications. This will require using image analysis software (e.g. Adobe Photoshop) and some darkroom work. Learning specific skills on the job is acceptable, but some familiarity with computers and illustration techniques will be preferred. We also need help with literature searches (w/ Ovid & Medline) and running a computer-based bibliography program (i.e. Papyrus). For this work, a background in college-level biology would be preferred. Time permitting, participation in quantitative data analyses would be an option, and a familiarity with statistical methods would be desirable. This job is available immediately for a period of 6-9 months. It would be suitable for a part-time college science major, a college graduate preparing for professional school or for a return to the job market after interruption of a career in science.
Contact: D. Kent Morest, M.D. Professor, Department of Neuroscience Director, Center for Neurological Sciences The University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030-3401 tel: 860-679-2645 fax: 860-679-8766 email: kent-at-neuron.uchc.edu web: http//www3.uchc.edu/~kent
-- Brandon H. Poe, Ph.D. Department of Neuroscience University of Connecticut Health Center Farmington, CT 06030 email: bpoe-at-ravenbrand.com
} Pauline, Here's my 2-cents.... } I just had a very bad run on our new Pelco CPD2--stuff came out visibly wet!
} } } } } } } It sounds as though you had not fully purged the transitional fluid } } } } } } } and/or you did not have a sufficient liquid CO2 level before you } } } } } } } heated.
And the o ring on the door is already stretched out of shape.
} } } } This sounds like you are getting the O-ring wet with your transitional } } } } fluid (ethanol?) when you are loading the chamber.
These problems will persist unless care is taken when loading samples and doing the "run". CPD is not something you can do well without paying close attention to each step no matter whose unit you use.
As a "veteran" of some of these same problems, with a different brand unit than yours, I speak from painful experience!
Good luck, Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
I have put images up on my wwwsite for your examination. Again, the Coolpix 800 was mounted on a 1X C-mount with the Nikon CP adapter.
A full-size crossed polar image of biotite which exhibits only minor vignetting: http://darkwing.uoregon.edu/~mshaf/dogsci/biotite-x.jpg
... the same image reduced in size: http://darkwing.uoregon.edu/~mshaf/dogsci/biotite-x-s.jpg
... the same biotite image ... uncrossed polars (no analyzer) ... which exhibits the problem vignetting because the CP800 stopped down its aperture: http://darkwing.uoregon.edu/~mshaf/dogsci/biotite-nx-s.jpg
A interference figure (calcite): http://darkwing.uoregon.edu/~mshaf/dogsci/bertrand.jpg
I had previously implied the CP800 would be a very low cost alternative for putting a "pro-sumer" digital camera on a microscope ...~US$800. Apparently I was wrong .. only under some lighting conditions will it work correctly. You do need one of the CP9xx series and use their "aperture priority" mode in most cases of illumination.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
Pauline - A user had a similar problem on a recent run using our stalwart Tousimis Samdri unit that has been in use for at least 20 years. The problem was due to an operator error - the purge valve was accidentally left closed so that the CO2 flow was too little or non-existent. The next run was perfect.
Good luck. Jill
Jill Verlander Reed, D.V.M. Associate Scientist Director, College of Medicine Electron Microscopy Core Facility University of Florida P.O. Box 100215 Gainesville, FL 32610 verlaj-at-medicine.ufl.edu Phone: (352) 846-0820 Fax: (352) 846-3299
Sorry to bother the list over this, but I received an email from Steve Poe, USDA, APHIS, responding to a question posted to the microscopy list. I tried to reply to the address given in his message:
Steve.R.Poe%aphisn1-at-hq.usda.gov
but my message was returned with a "user unknown" error.
If anyone has an email address for Steve other than the one above, please let me know.
Thanks, Ed
Ed Bachmann Odum Institute for Research in Social Science Manning Hall CB 3355 University of North Carolina at Chapel Hill Chapel Hill, NC 27599-3355 (919) 962-0512
Thanks for the replies to my question about thin films grown on PMMA. My original attempts to do cross sections in the PIPs have not yielded much success. Mainly because my samples are highly allergic to both heat and acetone so normal things like low temperature wax on the dimpler are out ... my next plan was to use the microtome.
However, my attempts at reviving an old (1986) unused (no one here knows how long ago) Sorvall Microtome have been for naught! I finally located (I think) all the blown fuses, burnt out light bulbs and loose wires, but I don't think the cutting arm is retracting after the down stroke. The block face seems to be rubbing against the back of the knife on the return (upward) stroke and dragging water and cut sections from the boat up with it.
Also, all my attempts at locating the Sorvall Company have not proven fruitful. I get all kinds of web addresses, all in German (not my native tongue) and they all take me to the same error message (Maybe this whole project is just not supposed to happen). So if any one can help with an good address or a service number for Sorvall, I would say prayers in your name to the electron gods!
Does anyone know if there is available for the JEOL 840/733 spectros a multilayer which covers O to P, same as the TAP?
And about how much they cost?
I'm having some difficulty getting any real info from Osmic.
Are there any other suppliers?
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
} Also, all my attempts at locating the Sorvall Company have not proven } fruitful. I get all kinds of web addresses, all in German (not my native } tongue) and they all take me to the same error message (Maybe this whole } project is just not supposed to happen). So if any one can help with an } good address or a service number for Sorvall, I would say prayers in your } name to the electron gods! } } Thanks in advance for all your help. } Dorrance.
*************** Hi Dorrance,
Sorvall was bought years ago by a company called RMC (Research Manufacturing Company), which has just become part of Boeckeler Instruments in Tucson, AZ.
Boeckeler Instruments, Inc. 4650 South Butterfield Drive Tucson, AZ 85714
Tel: (520) 745-0001 Fax: (520) 745-0004
email: info-at-boeckeler.com
I'm not sure that they can help you much, since RMC stopped supporting many of the older instruments (Parts are no longer available). Give them a call though, you never know....
Lee
(I have no financial interest in Sorvall, RMC or Boeckeler)
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Dear Colleagues: I have made great contacts through this listserver in the past and hope that I can again this time. I am in search of a postdoctoral fellow to continue a program of research using modern optical imaging methods to follow membrane trafficking in vascular endothelial cells. It is a very exciting project that cuts across the fields of cell biology, immunology, and pathology, and uses techniques from standard transmission EM to tracking GFP-labeled proteins. If you have the right background and are interested, or know of a colleague who fits these criteria, please get in touch with me. The following is the text of an ad that appeared in the August 10 issue of Nature and the September issue of Nature Cell Biology:
Postdoctoral position in Membrane Trafficking Postdoctoral position open to study membrane trafficking in endothelial cells. The ideal candidate would have experience studying regulated membrane trafficking at the cellular and molecular levels using biochemical, microscopic, and molecular biology approaches. Experience with modern imaging systems is critical. Experience with endothelial cells is a definite "plus", but not necessary. The candidate should demonstrate excellent communication skills in English, be able to work independently, as well as to interact with a lively group of productive investigators studying leukocyte-endothelial cell interactions. We are based at the Weill Medical College of Cornell University in the nicest and safest area of New York City. We interact extensively with investigators Weill as well as at The Rockefeller University and Memorial Sloan-Kettering Cancer Center, which are both just across the street. The position is fully-funded and available immediately.
Submit CV and names (and contact numbers) of three references to:
William A. Muller, MD, PhD Department of Pathology and Program in Immunology Box 69 Weill Medical College of Cornell University 1300 York Avenue New York, NY 10021 e-mail: wamuller-at-med.cornell.edu
Thank you very much for your help.
Sincerely, Bill Muller
William A. Muller, MD, PhD Associate Professor of Pathology Weill Medical College of Cornell University 1300 York Avenue New York, NY 10021
Why do you not try to section your thin film samples in a cryostat, we have manufactured and supplied many for this application with great success.
Best Regards
Alan Bright
Bright Instrument Co.Ltd. St Margaret's Way Huntingdon PE18 6EB England
Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: AlanBright-at-brightinstruments.com Web Site: www.brightinstruments.com
-----Original Message----- } From: McLean, Dorrance [mailto:dmclea-at-sandia.gov] Sent: 30 October 2000 20:23 To: 'microscopy'
Dear All,
Thanks for the replies to my question about thin films grown on PMMA. My original attempts to do cross sections in the PIPs have not yielded much success. Mainly because my samples are highly allergic to both heat and acetone so normal things like low temperature wax on the dimpler are out ... my next plan was to use the microtome.
However, my attempts at reviving an old (1986) unused (no one here knows how long ago) Sorvall Microtome have been for naught! I finally located (I think) all the blown fuses, burnt out light bulbs and loose wires, but I don't think the cutting arm is retracting after the down stroke. The block face seems to be rubbing against the back of the knife on the return (upward) stroke and dragging water and cut sections from the boat up with it.
Also, all my attempts at locating the Sorvall Company have not proven fruitful. I get all kinds of web addresses, all in German (not my native tongue) and they all take me to the same error message (Maybe this whole project is just not supposed to happen). So if any one can help with an good address or a service number for Sorvall, I would say prayers in your name to the electron gods!
Dear Evgeny, 1. Could you please tell more precise what signals you are going to use and what kind of objects you are going to investigate? 2. It depends on your vac system (with or without IGP, number of pirani gauges) but you will have to change some thresholds in the VAC electronics. 3.If you intend to insert the aperture in the objective lens (typical size 600-800micron) and use a BSD as a detector you should change: READY threshold in a high vac measuring (gray box behind the column) PREVAC thresholds in the VAC PCB 4. Anyway I wouldn't recommend to do this way because you will get problems with vacuum system - it's very sensitive.
Good luck,
Dmitri
Evgeny Zhikharev wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear colleagues, } I would like to carry out experiment of irradiating an object with } electrons in presence of gas media (about 1 torr) and intend to use } CamScan4 SEM for } this purpose. This device doesn't contain low vacuum accessories, so I } hope to make them by myself. Could you tell me } which are the tipical holes sizes in the differential pumping apertures, } the distance between them and expected pressure increasing in } diff. pump entrance. } Thanks in advance. } Eugeny Jikharev, } Physics & Technology Institute of Russian Academy of Sciences, } Moscow. } }
Ritchie Sims wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi, Listers } } Does anyone know if there is available for the JEOL 840/733 spectros } a multilayer which covers O to P, same as the TAP? } } And about how much they cost? } } I'm having some difficulty getting any real info from Osmic. } } Are there any other suppliers? } } cheers } } rtch } } Ritchie Sims Phone : 64 9 3737599 ext 7713 } Department of Geology Fax : 64 9 3737435 } The University of Auckland email : r.sims-at-auckland.ac.nz } Private Bag 92019 } Auckland } New Zealand Ritchie, Try Jim Nicholino at
JNicolin-at-xraydetectors.com
His regular crystals are terrific and I imagine if he doesn't supply multilayer crystals, he knows who does.
I am looking for references (current if possible) for thermal imaging using a thin insulating layer or other thermal imaging techniques that have a minimum of one micron spatial resolution. I am currently working with ridge lasers and would like to characterize the temperatures at the facet and along the ridge. Any help would be appreciated.
I am also looking for a beginner to advanced text on EBIC imaging/contrast mechanisms.
Yes, you can process an emulsion by freezing the sample, fracturing it and viewing it it by cryoSEM (Low Temperature SEM). The sample is kept frozen on a cold stage.
Dave
On Tue, 31 Oct 2000 08:31:14 +0100 (WAT) "O. O. Ilori" {sojilori-at-oauife.edu.ng} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Is it possible to view an emulsion under a SEM? By emulsion I mean } particles suspended in a matrix. } If possible, how? } } } Mr. O. O. ILORI } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING } OBAFEMI AWOLOWO UNIVERSITY, } ILE-IFE, OSUN STATE } NIGERIA. } } email: sojilori-at-oauife.edu.ng } } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Long time no hear from me. Did you miss me? I am now on the East Coast in Washington, DC and am looking for someone to be a EM tech. with me. Here is the job announcement:
TEM position available at George Washington University. The Department of Pathology is hiring a TEM technician. Must have experience with embedding, thick and thin sectioning, scoping and darkroom work. Immunochemistry experience a plus. Bachelor's degree and 2 years experience required. Excellent tuition benefits available. If interested please check the GW human resources web site at: www.gwu.edu/cgi-bin/hrs/vacancies Position title= Electron Microscope Technologist 2 Or contact me at patpxs-at-gwumc.edu or 202-994-2930
This is a clinical laboratory and we work mostly on clinical samples, though we do have some research samples come through (mostly HIV and AIDS related). The lab is pretty low key, but we do have a high standards.
George Washington University is smack dab in the heart of downtown Washington, DC. Walk a few blocks and you're at the White House (my friend even got to pet Buddy the Prez's dog), the mall with all the monuments and all sorts of other cool stuff.
The tuition benefit basically means you can take courses at GW for close to free (pay a reg. fee and buy books). Spouses and kids are eligible for a more limited benefit. Salary commensurate with experience, yada, yada, yada.
So if you're interested, please contact me.
Paula Sicurello patpxs-at-gwumc.edu 202-994-2930 (phone) 202-994-2518 (fax)
I have semi-thin sections of a sample embedded in methacrylate resin containing alginate and flour among other things. Could anyone give me advice how to stain the alginate for light microscopy?
a word of caution - unless GREAT care is taken in the freezing process there will be significant production of artifactual structure.... i.e. just like freezing biological tissue. See any of the many freeze fracture/freeze etch TEM references.
Bill Miller
At 09:19 AM 10/31/00, Patton, David wrote: } Microscopy-at-sparc5.microscopy.com
I spoke or wrote too soon regarding a source for Sorvall microtome parts. The following company took over the Sorvall product line from Ventana/RMC:
Boeckeler Instruments, Inc. 4650 South Butterfield Dr. Tucson, AZ 85714 Ph: 520-745-0001
Again, I have no connection with Ventana or Boeckeler Instruments. I just want to fix our Sorvall microtomes.
Greg Lum EM Facility San Francisco State University Ph: 415/338-1345 Email: glum-at-sfsu.edu
Greg Lum Computer/Microscopy Consultant Electron Microscopy Lab Mgr Department of Biology College of Science & Engineering San Francisco State University Ph: 415/338-1339 Fax: 415/338-2295 EMail: glum-at-sfsu.edu
We are thinking of purchasing a Kodak EDAS 290 Electrophoresis system. Does anyone have any experience with this system ( pros & cons), or can recommend one that they are happy with.
We currently doing some EM work on rat cultured hippocampal cells. The purpose of this experiment is to visualize individual synaptic vesicles by transmission electron microscopy. The problem we had is that the resolution/contrast is not high enough to distinguish the synaptic vesicles.
The following is what we did, cells were cultured on coverslips, they were fixed in situ in 2% glutaraldehyde for 1 hr to 24 hrs, postfixed with 1% OsO4 for 1 hour, and en bloc stained with 2% aqueous uranyl acetate for 30 min. dehydrated in ethanol and embedded in Polybed812. 40-50 nm sections were stained by 5% uranyl acetate and lead citrate prior to EM observation.
I would appreciate it very much if someone would provide suggestions on section staining or cell processing.
Xinran
******************************************** Xinran Liu, M.D., Ph.D. Center for Basic Neuroscience The University of Texas Southwestern Medical Center at Dallas 6000 Harry Hines Blvd., NA4.214A Dallas, TX 75390-9111 Phone: (214) 648-1830 Fax: (214) 648-1801 E-mail: xinran.liu-at-utsouthwestern.edu
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