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From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 30 Sep 2000 22:40:24 -0700
Subject: Coolpix 990 to Axioskop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone tried interfacing the Zeiss Axioskop 1
to Nikon CoolPix 990 with success? How did you
do it? Were the results good?

Anyone tried the Fuji FinePix S1 Pro yet with
success?

gary g



From daemon Sun Oct 1 12:56:51 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 2 Oct 2000 06:41:46 GMT+1200
Subject: Platinum Wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Can anybody suggest a supplier from whom I can buy some 0.001"
diameter Pt wire?
Please include e-mail address if possible.

thanks

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Tue Oct 3 00:37:05 2000



From: Jason Verie :      dont8-at-planet-mail.com
Date: Mon, 02 Oct 2000 21:26:07 -0500
Subject: Your Cards #7DF0

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


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************************************************************
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************************************************************




From daemon Mon Sep 4 09:08:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Wed, 4 Oct 2000 09:07:12 -0500
Subject: Testing 123

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html






From daemon Wed Oct 4 09:12:29 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Wed, 4 Oct 2000 08:52:59 -0500
Subject: Administrivia: Server should be back on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

Sorry all but it looks like I screwed up again. Over the weekend
I was editing the filtering software trying to increase the
SPAM/Junk Mail rejection capabilities..... Well, I manaaged to stop
all mail to the List which means I was 100% effective
in filtering the Junk Mail!! ;-)

In any event, I think things are back on track, and working again.
If you tried to post anything between Oct 1 and today
you should consider it as being permanently lost in the Ether..

Just resubmit them...

BTW, thanks to all those people who started sending me Email
saying " I haven't gotten any mail in the last few days" it started
me looking for problems. I guess it's good to know you all miss
your Daily Microscopy Email-Fix. Almost sounds addictive.
(Hmmm... too bad I can't bottle it an make a few $$)

Cheers..

Nestor
Your Friendly Neighborhood SysOp.





From daemon Wed Oct 4 10:08:00 2000



From: s_woodland-at-madasafish.com
Date: Wed, 4 Oct 2000 10:04:39 -0500
Subject: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


---------------------------------------------------------------------------

Email: s_woodland-at-madasafish.com
Name: Simon Woodland
School: Kaskenmoor School

Question: Where can I get some diatoms to put under our microscope? How
should they be prepared and mounted.

---------------------------------------------------------------------------




From daemon Wed Oct 4 10:34:46 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 04 Oct 2000 11:30:39 -0400
Subject: Flexible TLC prep for SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have an application for separating various size particles using Thin
Layer Chromatography.
Question, has anyone prepped the polyester backing, with silica gel
coating for SEM?

I have a couple hypothetical protocols I could try but I thought I would
send this question out to the masses, to see if anyone has had
experience examining TLC plates in the SEM (regluar SEM not an ESEM or
VPSEM).

Thanks in Advance,
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)




From daemon Wed Oct 4 11:06:11 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 4 Oct 2000 11:02:47 -0500
Subject: SEM: ISI HV Power supply (resending)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


A colleague on campus is having a problem with their ISI Alpha 9 SEM.

They have diagnosed a failure of the high voltage power supply.
Specifically, the gun side of the multi-tap power supply has been
fried.

So, they are looking for the power supply for the Alpha 9 or an ISI
Mini SEM. If anyone has such a component (or even the whole SEM) for
sale or donation, please contact me.

Thank you.

John B.

####################################################################
John J. Bozzola, Ph.D., Director
IMAGE (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################


From daemon Wed Oct 4 11:14:58 2000



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Wed, 4 Oct 2000 11:11:36 -0500
Subject: TEM position open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



} The Chemical Technology Division of Argonne National Laboratory has an
} opening for a staff microscopist to perform transmission electron
} microscopy in support of the nuclear fuel cycle, waste management, and
} other programs as required. This person will also develop new processes
} and/or equipment and other technology to support the programs, and
} interface with sponsors to maintain existing support and develop new
} initiatives.
}
} This position requires a Ph.D. in chemistry, materials science, or
} related discipline and 2-5 years of experience (or equivalent).
}
} For further information or to send resume, contact David Chamberlain by
} electronic mail : chamberlain-at-cmt.anl.gov.
}
}


From daemon Wed Oct 4 12:21:49 2000



From: NVo-at-IGC.com
Date: Wed, 4 Oct 2000 13:15:58 -0400
Subject: Ge vs Si detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listners:

Any comments on which would make a better detector, Ge or Si ? only PGT
sells Ge detectors and I'm aware of the decrease in resolution with Ge
detector. Could someone lists or details the benefits of having a Ge
detector as oppose to a Si one.

Sincerely

N.V.Vo



N.V.Vo, Ph.D.
Intermagnetics General Corporation
450 Duane Avenue
Schenectady, New York 12304
Phone: 518 346 1414 x 3107
Fax: 518 346 6080
E-mail: nvo-at-igc.com
www.igc.com



From daemon Wed Oct 4 13:02:41 2000



From: Sharon Coe :      slc6-at-lehigh.edu
Date: Wed, 4 Oct 2000 13:10:48 -0500
Subject: Faculty recruitment at Lehigh

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Simon
I am not a diatom expert, but I know someone who is. Put your
question to Professor David Mann at the Royal Botanic Garden
Edinburgh. He eats, sleeps and breathes diatoms (maybe I
exaggerate a little!). His email address is
d.mann-at-rbge.org.uk

good hunting
Chris Jeffree

} From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com
Date sent: Wed, 4 Oct 2000 10:04:39 -0500
To: Microscopy-at-sparc5.microscopy.com


Faculty Position
Department of Materials Science and Engineering
LEHIGH UNIVERSITY

Lehigh seeks to fill a tenure-track position, at the level of associate
or assistant professor, in Materials Science and Engineering. Research
interests in any area of materials will be considered but the person
selected will have a major commitment to the use of electron microscopy.
The Materials Department at Lehigh has research strengths in areas of
ceramics, metals, polymers and electronic materials. Details can be
found from the faculty pages at the departmental web site:
http://www.lehigh.edu/~inmatsci/inmatsci.html. It will be considered an
advantage if the research interests of the applicant are related to
existing research interests in such a way that collaborative work is
promoted. Lehigh runs an outstanding electron microscopy facility.
Favorable consideration will be given to candidates whose research will
involve substantial use of electron microscopy, especially when the
microscopy is innovative rather than routine. Lehigh is committed to
recruiting, retaining and tenuring women and members of minority groups.
Please submit an application by December 15, 2000, to Sharon Coe,
Department of Materials Science and Engineering, Lehigh University, 5
East Packer Avenue, Bethlehem PA 18015-3195, USA (slc6-at-lehigh.edu). To
discuss the post contact Alwyn Eades (jae5-at-lehigh.edu) at the same address.

==================================================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
==================================================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
==================================================================

The box said "This program requires Win 95/98/NT or better..." so I bought
a G3 Mac

==================================================================




From daemon Wed Oct 4 13:40:06 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Wed, 4 Oct 2000 13:28:51 -0500 (CDT)
Subject: MMS October Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The Minnesota Microscopy Society will be holding it's annual Fall Kick-Off event
on Thursday, October 12, 2000

This will follow the traditional format of a dinner followed by a talk, which
this year is:

"Nature's Jewels and Scientific Tools: Confessions of a Career Diatomist"

by Mark B. Edlund from the Science museum of Minnesota.


Abstract:

Diatoms are single-celled or colonial micro-organisms that possess a cell wall
of ornamented opaline silica (biologically-produced glass) and are commonly
referred to as the golden-brown algae. It is the wall ornamentation and shape
that are used to identify the nearly 25,000 species of living diatoms. Diatoms
were "discovered" within the first hundred years of microscopy; Leeuwenhoek
himself may be credited with one of the earliest illustrations (1703). Since
that time, diatoms have been the jewels, the postage stamps, and the tools of
amateur and professional diatomists. Microscopical societies burgeoned in the
latter half of the 19th century with members actively exchanging diatomaceous
material, slides, and methods and discussing publications and exsicattae.
Diatoms figured strongly in the development of light microscopy as test
organisms for resolving power; even today, many microscopists have an
Amphipleura pellucida slide in the drawer. Transmission electron microscopy
revealed much-needed knowledge on the cytology and mechanism of cell wall
formation in diatoms, but it has been the scanning electron microscope that has
truly changed the field. Ultrastructural details seen in the SEM coupled with
"rediscovery" of beautiful cytological work done in the late 1800's and early
1900's radically changed our modern understanding of diatom systematics. Lastly,
diatoms have proved to be excellent indicators of water quality and
environmental change. Whereas descriptive and biodiversity studies are still
common, applied methods now dominate the current scientific efforts.

Speaker: Mark B. Edlund, Research Scientist
Science Museum of Minnesota

Mark Edlund has a PhD in Natural Resources from the University of Michigan, and
has been specializing in diatoms for the last ten years. Besides field work in
the United States, he has done work at Lake Baikal in Siberia, and recently in
Mongolia.

Program:
5:30-6:00 PM Social hour
6:00-7:00 PM Dinner
7:00-7:15 PM Business meeting
7:15-8:15 PM Speaker

During the social hour, wine, cheese and crackers will be served.
The cost of the meal is $20 per person.

Location:
Cherrywood Room, 2nd Floor, St. Paul Students Cente,r St Paul Campus, University
of Minnesota, 2017 Buford Ave., St. Paul

Make Your Reservations Today

An accurate head count for the dinner is an absolute necessity. Contact Stuart
McKernan by Friday, October 6, to make your reservation (612-624-6009, or
stuartm-at-tc.umn.edu).




__________________
Stuart McKernan stuartm-at-tc.umn.edu
Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368



From daemon Wed Oct 4 14:21:52 2000



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 4 Oct 2000 14:17:25 -0500
Subject: Re: Ask a Microscopist - Diatomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have not tried this source, but have heard:

You might try a swimming pool supply store. Look for Diatomaceous Earth
filter media. As to preparation... Techniques will depend on the type of
microscopy employed. If using a high vacuum electron microscope, an
electrically conductive film will need to be applied to the diatoms which
have been (typically) sprinkled onto double faced tape. The tape will not
have to be conductive (if it is - so much the better) if the whole mount is
conductively coated.

Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

Me: http://home.att.net/~woody.white


From daemon Wed Oct 4 14:50:34 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Wed, 04 Oct 2000 13:43:21 -0600
Subject: messages

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Is there a problem with the server? I haven't received any messages in the last couple of days. I checked with our server and everything seems to be O.K.
Did my address get taken off by accident? In case it was my address is: prutledge-at-ars.usda.gov
Thanks,

Phillip Rutledge
USDA/ARS
Aquatic Animal Research Lab.
151 Dixon Drive
Chestertown, MD 21620
voice: 410.778.4136 or 2120
fax: 410.778.4399
e-mail: prutledge-at-ars.usda.gov


From daemon Wed Oct 4 14:50:34 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 4 Oct 2000 14:50:43 -0400
Subject: RE: Pt wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I would try the two following companies as possible sources of fine
platinum wire:

GoodFellow, P.O. Box 628, Doylkestown, PA 18901-9975
FAX: 1-800-283-2020

Refining Systems Inc., P. O. Box 72446, Las Vegas, NV 89170
FAX: 702-368-0933

Both sell high purity and specialty metals in a variety of sizes and shapes.
Good luck,
WCB

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Wed Oct 4 14:51:53 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 4 Oct 2000 15:50:00 -0400
Subject: Re: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


At 10:04 AM -0500 10/4/00,
"s_woodland-at-madasafish.com"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Have you tried Carolina Biological? They have all kinds of pre-mounted
slides for sale.

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Wed Oct 4 16:35:14 2000



From: William Carmichael :      wcarmichael-at-madison.tec.wi.us
Date: Wed, 04 Oct 2000 16:31:03 -0500
Subject: powder TEM diffraction sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Anyone know of a easy, simple way to produce nice powder diffraction samples for illustrating the powder diffraction method for EM students? We use the International Centre for Diffraction Data For student identification of unknown samples. I have tried several methods that usually result in non-electron transparent samples (our TEM KV max is only 120). Any ideas?


_______________________________

Bill Carmichael
Electron Microscopy Faculty

Madison Area Technical College
3550 Anderson St.
Madison, WI 53704
608-243-4309

wcarmichael-at-madison.tec.wi.us
http://electron-microscopy.madison.tec.wi.us




From daemon Wed Oct 4 16:58:33 2000



From: Jeff Thole :      thole-at-macalester.edu
Date: Wed, 04 Oct 2000 16:56:27 -0500
Subject: Electronics/Electron Microscopy Position at Macalester College

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Macalester College (St. Paul, Minnesota) has a job opening which involves
looking for someone with a microscopy background (as well as electronics
skills). The job description is posted at:
http://www.macalester.edu/~hr/jobs/job551.html

The description is quite brief, but Macalester has both a Zeiss EM109 TEM
and a Zeiss DSM960A SEM. Maintenance and instruction for students/faculty
on these machines will be one aspect of this position.

Regards,

Jeff Thole



__________________________________________________

Jeff Thole - Geology Laboratory Supervisor and Instructor
Geology Department, Macalester College
1600 Grand Avenue, St. Paul, MN 55105
(ph. 651-696-6426, fax. x6122, email thole-at-macalester.edu)
__________________________________________________




From daemon Wed Oct 4 17:31:11 2000



From: COURYHOUSE-at-aol.com
Date: Wed, 4 Oct 2000 18:27:49 EDT
Subject: Re: Ask a Microscopist - Diatomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Yes, indeed! You can buy a sack of the stuff there that will keep you busy
for all of eternity!

Also check with your neighbors where you live if they have pools and one of
them might spare a handful!

Ed Sharpe archivist for SMECC

{ { Subj: Re: Ask a Microscopist - Diatomes
Date: 10/4/00 3:20:58 PM US Mountain Standard Time
From: nwwhite-at-mcdermott.com (White, Woody N.)
To: woodland-at-madasafish.com ('woodland-at-madasafish.com'),
Microscopy-at-sparc5.microscopy.com ('Microscopy-at-sparc5.microscopy.com')

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


I have not tried this source, but have heard:

You might try a swimming pool supply store. Look for Diatomaceous Earth
filter media. As to preparation... Techniques will depend on the type of
microscopy employed. If using a high vacuum electron microscope, an
electrically conductive film will need to be applied to the diatoms which
have been (typically) sprinkled onto double faced tape. The tape will not
have to be conductive (if it is - so much the better) if the whole mount is
conductively coated.

Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

Me: http://home.att.net/~woody.white

} }


From daemon Wed Oct 4 18:51:30 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 4 Oct 2000 19:47:15 -0400
Subject: RE: powder TEM diffraction sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Take an oxide powder, grind it up with mortar and pestle and take a carbon
coated grid and swipe it across the fines. You can also use two glass
slides to grind the powder up.

You can evaporate a number of materials onto a carbon coated grid or onto a
cleaved NaCl sample and then float that off on water onto a grid.

You could smoke a Mo wire in air to provide crystals if you leave it in the
smoke long enough, you will probably have enough to cover the grid. Heat
the wire across the terminals of an evaporator or heat with a torch to
generate the white smoke. You could also burn some Mg and get MgO crystals.

If you don't have sufficient particles in a single diffraction pattern to
give you rings, take multiple exposures from different areas on the same
piece of film.

Incidentally, the MoO3 samples will provide you with a rotation calibration
sample.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: William Carmichael [mailto:wcarmichael-at-madison.tec.wi.us]
} Sent: Wednesday, October 04, 2000 5:31 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: powder TEM diffraction sample prep
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Anyone know of a easy, simple way to produce nice powder
} diffraction samples for illustrating the powder diffraction
} method for EM students? We use the International Centre for
} Diffraction Data For student identification of unknown
} samples. I have tried several methods that usually result in
} non-electron transparent samples (our TEM KV max is only
} 120). Any ideas?
}
}
} _______________________________
}
} Bill Carmichael
} Electron Microscopy Faculty
}
} Madison Area Technical College
} 3550 Anderson St.
} Madison, WI 53704
} 608-243-4309
}
} wcarmichael-at-madison.tec.wi.us
} http://electron-microscopy.madison.tec.wi.us
}
}
}


From daemon Wed Oct 4 19:15:31 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 04 Oct 2000 17:34:21 -0700
Subject: Re: Ask a Microscopist - Diatomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Message-ID: {39DBE9C8.7597-at-netrax.net}


What is the difference between diatoms and radiolaria (Actinopods)?
They "look" the same to me.

gary g.


At 12:17 PM 10/4/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Oct 4 19:52:33 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Wed, 04 Oct 2000 17:23:25 -0700
Subject: Bio Rad Rep in US

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have a BioRad SC500 coater.
Can anyone give me the local US rep for service?

Thank You,

Earl Weltmer



From daemon Wed Oct 4 19:56:28 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 4 Oct 2000 17:53:50 -0700
Subject: Re: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



} ---------------------------------------------------------------------------
}
} Email: s_woodland-at-madasafish.com
} Name: Simon Woodland
} School: Kaskenmoor School
}
} Question: Where can I get some diatoms to put under our microscope? How
} should they be prepared and mounted.
}
} ---------------------------------------------------------------------------

Simon -

You can go to a big home supply store and ask for "diatomaceous earth";
it's used for a lot of filtering applications. You'll get a big sack, so
you can use it up by ordering the Lawrence Hall of Science/GEMS manual
"River Cutters" (see the Prtoject MICRO URL below), which uses the same
materiel to make a model stream bed. Or you can order a prepared slide
from a variety of suppliers, Or you can contact Joe Neilly
{joe.p.neilly-at-abbott.com} , keeper of Project MICRO's sandpile, and ask for
samples of foraminefera sand or the very exotic "star sand". Preparation?
Minimal. "Microscopic Explorations", MSA's GEMS manual, will tell you how
to make filecard and tape slides that will work well.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Oct 4 21:10:29 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Thu, 5 Oct 2000 13:07:54 +1000
Subject: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
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The source I have used in the past is kitty litter. It usually has a lot
of diatomaceous earth in it.

have fun,


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Wed Oct 4 21:55:54 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Wed, 4 Oct 2000 19:52:12 -0700 (PDT)
Subject: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
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I'm trying to solve a high resolution noise problem with a XL-30
Phillips FESEM. The room that it is in was recently checked and was ok,
accept for some acoustic noise. To fix the noise I put up 3 inch sound
proofing foam and quieter ducting for the air condition. The noise
showed up in an image by a streak about 5 jaggies. After
putting up the foam the noise was reduced, but it is still there. The
noise is the same for 4mm working distance or a 10mm working distance.

Any one had a similar problem or have any advice?

Thanks,
Ben




#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \





From daemon Wed Oct 4 22:20:58 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 4 Oct 2000 22:22:38 -0500
Subject: Re: Ask a Microscopist - Diatomes

Contents Retrieved from Microscopy Listserver Archives
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Gary,

There's a *lot* of difference. About that between a Redwood and an
Elephant. Or more. And as distinctive.

Diatoms are about as good a plant as can be found among the
protistans. They look like more-or-less fancy silicate pillboxes with
lots of little holes.

Radiolaria are amoeboid beasts with a complicated silicate internal
"skeleton". They tend to be nested, holey spheres, usually with a
more-or-less complex profusion of spikes, needles, and the like.

Crude descriptions, but brief. The best way to see the difference is
to get a copy of Ernst Haeckel's book published by Dover -- "Arts
Forms in Nature". Beautiful drawings of many different organisms,
including Radiolaria and Diatoms.

Phil

} What is the difference between diatoms and radiolaria (Actinopods)?
} They "look" the same to me.
}
} gary g.
}
--
*** Be famous! Send a Tech Tip or article to Microscopy Today! ***
Philip Oshel
Technical Editor, Microscopy Today
P.O. Box 620068
Middleton, WI 53562-0068
Voice: days (608) 263-4162, evening (608) 833-2885
Fax (608) 836-1969 (please make sure my name is on any fax)

Address for UPS, FedEx, or other couriers:
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
fax: (608) 262-7420 (dept. fax)

peoshel-at-facstaff.wisc.edu


From daemon Wed Oct 4 22:56:49 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wed, 4 Oct 2000 23:54:09 -0400
Subject: Fw: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

What certified (NIST traceable?) TEM mag. calibration samples are available
for 20K to over 100K range? Diffraction grating replica sample will not
provide sufficient accuracy- too few grating periods "fit" in the image at
higher magnifications.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax


This message is made of 100% recycled electrons.



From daemon Wed Oct 4 23:20:11 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Wed, 4 Oct 2000 23:16:09 -0500
Subject: : Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
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Simon:

Try Wards Natural History Establishment in upstate New York. The
exact address escapes me.

Sam Purdy
TechnicalCenter
National Steel Corp
Trenton MI


} ----------
} From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com
} Sent: 4, October 2000, 11:04 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: Diatoms
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} --------------------------------------------------------------------------
} -
}
} Email: s_woodland-at-madasafish.com
} Name: Simon Woodland
} School: Kaskenmoor School
}
} Question: Where can I get some diatoms to put under our microscope? How
} should they be prepared and mounted.
}
} --------------------------------------------------------------------------
} -
}
}
}




From daemon Thu Oct 5 00:37:14 2000



From: Anton Gutakovskii :      gut-at-thermo.isp.nsc.ru
Date: Thu, 5 Oct 2000 12:35:59 +0600
Subject: help for jem-4000

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers!

We have the problems with our JEM-4000EX: the ion pump SIP-1 is not OK!
The possible causes are:
1/- leak in vacuum system (after bake out of the SIP);
2/-some contamination into SIP (this SIP was go OK more 40,000 hour);
3/- ???
Does anyone know how to make testing of this situation?

How do to demount the pump, what order of actions?

I am not currently part of the list, so please mail to me directly.

--
Best regards,
Anton Gutakovskii mailto:gut-at-thermo.isp.nsc.ru




From daemon Thu Oct 5 03:25:38 2000



From: PETER HEIMANN :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Thu, 05 Oct 2000 10:21:37 +0200
Subject: Re: Coolpix 990 to Axioskop (or any other photomicroscope) "re-sent"

Contents Retrieved from Microscopy Listserver Archives
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Yes,
mounting the Coolpix 990 to any photomicroscope is absolutely
simple via the usual C-mount connection. For the coolpix you have
to buy at NIKON the microscope adapter ( here in germany this
adapter is around 1000.- DM or approx. 450.- US $; adapter is not
listed in Nikon catalogue; contact the microscope section of Nikon)
fitting on one side to the thread at the front of the lens and on the
other side to your microscope-specific C-mount adapter ( c-mount
adapter for Zeiss: buy the more expensive one which has the
possibility of correction of the focus plane).
The fantastic thing is that you can focus not only via the LCD-
screen of the camera ( possible, but not so easy in my opinion) but
via an external TV-Monitor in live modus, yielding really smashing
results with a resolution satisfying publication needs.
With my Coolpix sometimes the camera "hangs up" when the Auto-
power off mode is activated (de-activating it helps). Does anybody
have this problem as well ?? ( Contact me off-line).
What is lacking for the Coolpix 990 at the moment is a cable
release, but the Nikon people here in germany tell me it will be
available at the end of the year (probably very expensive).

All in all, the Coolpix is the first digital camera under 5000 US-$ I
have used or tested which is fully satisfying me in my daily lab
routine (photomicroscope (even fluorescence), binocular, makro-photographs)
especially due to its simple and clear operation, the possibility to
adjust everything (speed, aperture, focus, etc.) manually
and its excellent results.

Peter Heimann

**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************


From daemon Thu Oct 5 03:56:15 2000



From: Kevin Mackenzie :      nhi691-at-abdn.ac.uk
Date: Thu, 5 Oct 2000 09:55:41 +0100 (BST)
Subject: Scottish Microscopy meeting

Contents Retrieved from Microscopy Listserver Archives
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28th SCOTTISH MICROSCOPY SYMPOSIUM

http://www.abdn.ac.uk/emunit/smg2000.htm

Wednesday 15 November 2000, Moredun Institute, Edinburgh.

The annual symposium is supported by all area groups in Scotland.
The programme for this meeting is comprised of the following three
invited talks together with seven contributed talks on a wide
range of microscopy with general appeal and a poster display.
As in recent years, a comprehensive trade exhibition by leading companies
will be present. The Trade Exhibition, Poster Display and Catering
will all be together in one central area of this excellent venue.

15/11/2000 Programme

Multi-photon microscopy. Professor Alison Gurney, Dept.of
Physiology and Pharmacology, University of Strathclyde.

Ultrastructure eggshell quality assessment: a way forward for
captive breeding programmes. Avril Edmond, Dept.
Veterinary Anatomy, Glasgow.

Non-invasive imaging of plant tissues using the confocal laser
scanning microscope (CLSM) Dr. Karl Oparka, Unit of Cell Biology, SCRI,
Dundee.

Functional dynamics of plasmodesmata in sink/source transition
leaves. Alison Roberts, Unit of Cell Biology, SCRI, Dundee

COSMIC - a new interdisciplinary facility for advanced
spectroscopy, micromanipulation and imaging. Professor Wilson
Poon, Dept. of Physics and Astronomy, University of Edinburgh.

Imaging intracellular location of psychoactive drugs
using fluorescence microscopy. Richard Horobin, IBLS, Glasgow.

Scanning infra-red microscopy. Alison Coats, Dept. Chemistry,
Aberdeen University.

Antigen retrieval allows improved visualisation of V-ATPase
immuno-reactivity. Susan Lindsay, Caledonian University, Glasgow.

Techniques in Immunocytochemistry. Peter Jackson, Research
Histology Unit, Dept. of Histopathology and Molecular Pathology,
Leeds General Infirmary. Talk sponsored by DACO Ltd.


See web site for abstracts from previous meetings

http://www.abdn.ac.uk/emunit/smg99.htm

Kevin Mackenzie
Electron Microscope unit
Dept Zoology
University of Aberdeen
Tillydrone avenue
Aberdeen
AB24 2TZ
-----------------
Tel 01224 272847
Fax 01224 272396
email k.s.mackenzie-at-abdn.ac.uk





From daemon Thu Oct 5 04:26:41 2000



From: patrick gunning :      patrick.gunning-at-bbsrc.ac.uk
Date: Thu, 5 Oct 2000 10:16:18 +0100
Subject: Atomic Force Microscopy for Biologists

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I hope this isn't seen as an overt advert on the list server, I just want to
let people know that this new book is available and hope it may be of
general interest. Rest assured that I and my fellow co-authors get very
little of the sales proceeds!

A.Patrick Gunning
IFR Norwich

ATOMIC FORCE MICROSCOPY FOR BIOLOGISTS

by V J Morris, A R Kirby & A P Gunning

Atomic force microscopy (AFM) is part of a range of emerging microscopic
methods for biologists which offer the magnification range of both the light
and electron microscope, but allow imaging under the 'natural' conditions
usually associated with the light microscope. To biologists AFM offers the
prospect of high resolution images of biological material, images of
molecules and their interactions even under physiological conditions, and
the study of molecular processes in living systems. This book provides a
realistic appreciation of the advantages and limitations of the technique
and the present and future potential for improving the understanding of
biological systems.

Contents:
Apparatus
Basic Principles
Macromolecules
Interfacial Systems
Ordered Macromolecules
Cells, Tissue and Biominerals
Other Probe Microscopes

Readership: Undergraduates, postgraduates and researchers in biophysics.
352pp Pub. date: Dec 1999 Imperial College Press
ISBN 1-86094-199-0 US$51 / £32


From daemon Thu Oct 5 05:34:44 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Thu, 05 Oct 2000 03:36:26 -0700
Subject: Re: Coolpix 990 to Axioskop (or any other photomicroscope) "re-sent"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Peter,

I think one can use the "self timer" function of the Coolpix 990 to
expose an image without touching the camera body at the time of the
exposure.

I can think of no engineering reason why the cable release would need to
be expensive when it becomes available. I'll bet any electronics
tinkerer could make one for $30.

I'm quite happy with my Cool-Pix 990. If only I could remember 10% of
its function modes and how to access them.

Bart Cannon
Cannon Microprobe
Seattle



From daemon Thu Oct 5 07:14:30 2000



From: Jinguo Wang :      jqw11-at-psu.edu
Date: Thu, 05 Oct 2000 08:37:36 -0400
Subject: Enlarger or digital image system

Contents Retrieved from Microscopy Listserver Archives
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High-purity Germanium detectors provide *better* resolution than
Si(Li) detectors, and also have lower low-keV background. The
higher atomic number of Ge means they have greater x-ray
stopping power and therefore higher detection efficiency for the K
lines of the heavier elements, and they have therefore been thought
of as primarily useful at higher beam accelerating voltages for
heavy element analysis. However, this may only be one of their
advantages. The better resolution and lower background they offer
at low keV suggests they would also be good, and perhaps
preferable to Si for light element detection and peak discrimination
which could mean more options for low-kV excitation in Field
emission SEMs.

What are the flaws in this argument?
It would be interesting to hear comments on the above from anyone
who has direct knowledge and experience of both types of
detectors.

BTW besides PGT, both Gresham and Oxford sell Ge detectors. I
am not aware of any others
Chris



} From: "NVo-at-IGC.com"-at-sparc5.microscopy.com
To: Microscopy-at-sparc5.microscopy.com


Dear Listers:

I am trying to find an enlarger and digital image system to print the
conventional and HREM negatives. What kind of enlarger and digital image
system are better? manufacturer? prices?

Thanks a lot!

Jinguo Wang




From daemon Thu Oct 5 08:03:00 2000



From: rgriffin-at-eng.uab.edu
Date: Thu, 5 Oct 2000 07:54:39 -0500
Subject: Digital microscope camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


} I'm looking to replace my two year old Polaroid Digital Microscope camera
} (SCSI interface with color or black and white, 1600x1200 pixels). The
} chip has failed and I don't want to buy another Polaroid camera. I do
} primarily black and white photography from a Zeiss optical microscope for
} materials engineering applications. I need for the camera to save in tiff
} format without changing the dimensions of the objects. I don't need low
} light applications.
}
} Any suggestions?
}
} Thanks,
}
} Robin Griffin
}


From daemon Thu Oct 5 08:04:45 2000



From: Joseph Neilly :      joe.p.neilly-at-abbott.com
Date: Thu, 5 Oct 2000 08:04:03 -0500
Subject: Re: powder TEM diffraction sample prep

Contents Retrieved from Microscopy Listserver Archives
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Bill,

We have found a simple method for preparing drug particles for electron
diffraction. We suspend particles in a solvent by sonication for 2-3 seconds
and then let the suspension settle for 10-20 seconds. The largest, heavy
particles will settle first leaving the thin, light ones in suspension. Then
take a drop (20 ul) from the top of the solution, place it on a carbon filmed
grid and allow it to dry. The result is a distribution of particles laying
flat accross the grid. You need a solvent in which the particles are not
soluble. We typically use hexane.

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202
voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com


From daemon Thu Oct 5 08:16:50 2000



From: Xu, Yuhui (NHLBI) :      XuY-at-nhlbi.nih.gov
Date: Thu, 5 Oct 2000 09:10:48 -0400
Subject: TEM of DNA and Protein complex

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues:

I have two questions on TEM of DNA and protein complex:
1. which method is the best , or most suitable ,to study such samples?
Direct spray to the mica sheet and subsequent metal rotary shadowing, or
cytochrome c spreading with metal coating examined by TEM, or atomic force
microscopy?
2. Is there any computer software which is designed specifically for analyze
these type of images?

Any comments will be greatly appreciated.

Yuhui

Yuhui Xu, MD,PhD
EM Core, NHLBI, NIH


From daemon Thu Oct 5 08:23:32 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Thu, 5 Oct 2000 08:20:29 -0500
Subject: XRD Wanted

Contents Retrieved from Microscopy Listserver Archives
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This is a bit off the topic of microscopy, but most of us on this
server do work in facilities that have XRD units. If anyone is selling,
or knows of someone who is selling their XRD, please contact me. I am also
curious if anyone has had any experience with the new desk tops like
Rigaku has. I plan on demoing one in the future. At $50k-$60k, they are
very cost efficient compared to standard XRD units, but I am wary of their
performance as an analytical instrument. Lou Solebello




From daemon Thu Oct 5 08:49:51 2000



From: Linton Brown :      l.j.brown-at-stir.ac.uk
Date: Thu, 5 Oct 2000 14:47:42 +0100
Subject: Protocol for fish egg samples

Contents Retrieved from Microscopy Listserver Archives
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Dear members,
I am a PhD student and I use a subscriber to find an answer to a problem I
have.
I am trying to find a method to fix and process whole fish eggs in order to
cut sections for immunohistochemistry. Any suggestions?

Ioannis Vatsos
Institute of Aquaculture
University of Stirling
Scotland, UK


From daemon Thu Oct 5 09:13:29 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Thu, 05 Oct 2000 10:04:00 -0400
Subject: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
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hi all-

i've installed a 35 and have some issues with a saw-tooth distortion in the
scan. it stays constant regardless of KV, but the number of saw-tooths
(teeth?) in a frame increases with slower scan rates. i suspected
vibration so i mounted the roughing pumps on a big foam block.....no
change. i also cycled off the chiller unit with no change, so its probably
not mechanical vibrations, unless there is another vibration causing
component i'm overlooking.

the distortion is also visible in the data field alphanumerics (they shift
and shimmy a little) with the beam turned off, so i don't suspect a HV
instability problem.

can i logically deduce that there is an electrical field leakage that is
messing up the scan? or a ground loop? what about cable placement? or a
problem with the 100V variac (its within 3' of the column).

right now the column is a bit dirty and needs cleaning....i've never seen,
for example, dirty apertures cause this type of distortion though.

any suggestions would be appreciated!

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875


From daemon Thu Oct 5 09:13:34 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Thu, 05 Oct 2000 10:09:41 -0400
Subject: Re: Bio Rad Rep in US

Contents Retrieved from Microscopy Listserver Archives
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Earl,

We distributed the Polaron coaters for years. We can assist in spare
parts and perhaps service.

John Arnott


--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Microscope Supplies Since 1955



Earl Weltmer wrote:


} Hi all,
}
} I have a BioRad SC500 coater.
} Can anyone give me the local US rep for service?
}
} Thank You,
}
} Earl Weltmer


From daemon Thu Oct 5 09:24:19 2000



From: McCaffrey, John :      John.McCaffrey-at-nrc.ca
Date: Thu, 5 Oct 2000 10:19:28 -0400
Subject: Fw: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello Vitaly,

There currently is no NIST-traceable standard for high-magnification
TEM on the market. The next best thing is the MAG*I*CAL TEM calibration
sample, which is internally calibrated against the lattice spacing of
silicon, a fundamental constant of nature. This statement has been used
successfully in the past in fulfillment of the requirements for
certification and traceability. The MAG*I*CAL sample allows magnification
calibrations across the entire magnification range of TEMs, as well as the
camera constant calibration and the image/diffraction pattern rotation
calibration.

The sample is marketed by South Bay Technology, TEL: 800-728-2233,
1120 Via Callejon, San Clemente, CA 92673 USA, e-mail:
henriks-at-southbaytech.com It is also marketed through various microscopy
supply houses, (SPI, EMS, Pelco, etc.) if you have a favorite.

Your request again raises the concern that the microscopy community
needs a high-magnification TEM calibration sample that is NIST-certified or
NIST-traceable. NIST is currently considering investigating the need for a
high-magnification TEM calibration reference, but will not proceed unless
there is some interest shown from the user community. It would be helpful
to the community in general if you could write a note or send an email to
Ms. Nancy Trahey, stating the problem that you have encountered, and
reiterating the need for such a NIST-certified or NIST-traceable standard.
Her co-ordinates are listed below.

Ms. Nancy M. Trahey
Standard Reference Materials Program (232)
Engineering Mechanics (202), Room 113
NIST
100 Bureau Drive, Stop 2320
Gaithersburg, MD 20899-2320
Tel: (301) 975-2021
email: nancy.trahey-at-nist.gov

Disclaimer: I 'invented' the MAG*I*CAL TEM calibration sample and retain
all bragging rights; it is listed in the Guinness Book of Records as "The
World's Smallest Ruler".


John P. McCaffrey, Ph.D.
National Research Council of Canada
M-50, Montreal Rd.
Ottawa, Ontario
K1A 0R6 CANADA

tel: +613-993-7823
fax: +613-990-0202
email: john.mccaffrey-at-nrc.ca


-----Original Message-----
} From: Vitaly Feingold [mailto:vitalylazar-at-worldnet.att.net]
Sent: Wednesday, October 04, 2000 11:54 PM
To: .


Dear listers,

What certified (NIST traceable?) TEM mag. calibration samples are available
for 20K to over 100K range? Diffraction grating replica sample will not
provide sufficient accuracy- too few grating periods "fit" in the image at
higher magnifications.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax


This message is made of 100% recycled electrons.



From daemon Thu Oct 5 09:33:42 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 5 Oct 2000 07:21:11 -0700
Subject: RE: Ge vs Si detector

Contents Retrieved from Microscopy Listserver Archives
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N.V.Vo writes ...

} Any comments on which would make a better detector, Ge or
} Si ? only PGT sells Ge detectors and I'm aware of
} the decrease in resolution with Ge detector.
} Could someone lists or details the benefits of having a Ge
} detector as oppose to a Si one.

We are using a "Gem" detector from Oxford Instruments with
absoloutely no problems ... 115eV resolution, fast count rates, and
very little energy shift with varying time constants. Another benefit
is there are no escape peaks for x-ray lines 0~10keV. One drawback
may be if you intend to allow the detector to come up to ambient
temperatures at times. Si detectors seem to accommodate this, whereas
it was suggested at the time of purchase we absolutely not allow this
.. but I believe that was simply because the "Gem" was for the most
part untested in this respect. I have no idea of this detector's
ambient T survivability, we have always kept it at LN2 T.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Thu Oct 5 09:49:47 2000



From: carla_aiwohi-at-usgs.gov
Date: Thu, 5 Oct 2000 06:46:53 -0700
Subject: SEM Help needed on conductive adhesive

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with
Electrodag 502 for secondary electron imaging. The adhesive is a graphite
paint in MEK base (a substitute for TV Tube Koat). After ethanol
dehydration and CP drying in liquid CO2, the samples are relatively flat or
slightly curved. However, once attached to the stub the skins develop a
noticeable curl which leaves a gap between the stub surface and sample. In
some cases I can see the "trail" in the adhesive left by the skin as it was
curling! Filling the gap with more Electrodag is a waste of time because
it evaporates and shrinks back away from the sample (thankfully it doesn't
seem to produce more curling).

Could anyone suggest a better conductive adhesive? Also, is there any
difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based
media as to its effect on biological samples?

As an SEM novice any advice you could share with me is greatly appreciated.
Thank you very much,

Carla Aiwohi
Western Fisheries Research Center
Seattle, WA
ph: (206) 526-6282 ext.242
fax: (206) 526-6654



From daemon Thu Oct 5 10:09:34 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Thu, 5 Oct 2000 11:06:34 -0400
Subject: Re: powder TEM diffraction sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html







Anyone know of a easy, simple way to produce nice powder diffraction samples for
illustrating the powder diffraction method for EM students? We use the
International Centre for Diffraction Data For student identification of unknown
samples. I have tried several methods that usually result in non-electron
transparent samples (our TEM KV max is only 120). Any ideas?

Dear Bill,
The simplest way I know is to evaporate a layer of Au or Al onto a
formvar-covered grid. By spreading
the beam, you can get continuous rings, and by using a small selected area you
can get discrete spots at various positions around the rings, so you can
demonstrate directly that the rings consist of the summed intensities from many
crystalites of differing orientations. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Thu Oct 5 10:17:52 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 5 Oct 2000 11:16:34 -0400
Subject: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Ben,
Our Zeiss DSM982 FESEM is also sensitive to acoustic noise. "The quieter
the room the more noises we hear." There is no simple answer, just keep
trying different things. I was told one lab installed a switch to turn the
SEM PC cooling fans off for short periods of time during high resolution
work. That is a risky fix if you are over 50 years old.

We strategically padded all connections to the column and redirected the
room air flow but have not installed acoustic foam on the walls. Evenings
are quieter than days. At 300kx we can detect a backhoe 600 yards away.

Good Luck
Jim



} Date: Wed, 4 Oct 2000 19:52:12 -0700 (PDT)
} From: Ben Craft {bcraft-at-uci.edu}
} X-Sender: bcraft-at-taurus.oac.uci.edu
} To: Microscopy-at-sparc5.microscopy.com
} Subject: High Resolution noise
} MIME-Version: 1.0
} }
}
} I'm trying to solve a high resolution noise problem with a XL-30
} Phillips FESEM. The room that it is in was recently checked and was ok,
} accept for some acoustic noise. To fix the noise I put up 3 inch sound
} proofing foam and quieter ducting for the air condition. The noise
} showed up in an image by a streak about 5 jaggies. After
} putting up the foam the noise was reduced, but it is still there. The
} noise is the same for 4mm working distance or a 10mm working distance.
}
} Any one had a similar problem or have any advice?
}
} Thanks,
} Ben
}
}
}
}
} #######
} #####\_O -Ben Craft-
} ####/\/}
} #### /"
} ### \
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Thu Oct 5 10:53:07 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Thu, 05 Oct 2000 10:49:06 -0500
Subject: Re: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Simon,

You could prepare your own. I find that the diatoms in diatomaceous earth are
usually broken and like to prepare my own. You will need a plankton net, some
potassium dichromate, and 30% peroxide. Once the diatoms are collected
(plankton net tows from shoreline) you can clean them using the 30% peroxide
and potassium dichromate. Place the diatoms in a large beaker (and I mean
large here) then pour in 15 to 20 mls of peroxide followed by a large pinch of
the potasium dichromate. The resultant reaction oxidizes organics leaving the
glass frustules of the diatoms unharmed. It is better to be cautious with the
amounts that you use as this is an exothermic reaction, sometimes violently
so. Not that you'll have an explosion, but I've had to clean up boil overs on
a number of occasions. Once the reaction subsides and the solution turns a
golden yellow color it is just a matter of washing the diatoms. There are a
couple of ways to do this. You could centrifuge the diatoms, then throw out
the liquid, add water, centrifuge, throw out the liquid, etc. or you could fill
the large beaker with water and let the diatoms settle to the bottom (1-2hrs),
carefully pour off the fluid, and add more water, let the diatoms settle, etc.
The diatoms will appear as a milky white residue in the bottom of your
container (you may have to concentrate the material to really see this). If
you need any more info please feel free to contact me.

Greg

"s_woodland-at-madasafish.com"-at-sparc5.microscopy.com wrote:

} ---------------------------------------------------------------------------
}
} Email: s_woodland-at-madasafish.com
} Name: Simon Woodland
} School: Kaskenmoor School
}
} Question: Where can I get some diatoms to put under our microscope? How
} should they be prepared and mounted.
}
} ---------------------------------------------------------------------------

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Thu Oct 5 10:57:33 2000



From: jim quinn :      jquinn-at-doL1.eng.sunysb.edu
Date: Thu, 5 Oct 2000 11:51:05 -0400
Subject: Re: Coolpix 990 to Axioskop (or any other photomicroscope) "re-sent"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Bart and Peter

Besides Nikon's own release (EU-1 wired remote control) for
the CoolPix990, you can purchase a third-party release cable
from the CKCpower.com website.

Jim


}
} Hi Peter,
}
} I think one can use the "self timer" function of the Coolpix 990 to
} expose an image without touching the camera body at the time of the
} exposure.
}
} I can think of no engineering reason why the cable release would need to
} be expensive when it becomes available. I'll bet any electronics
} tinkerer could make one for $30.
}
} I'm quite happy with my Cool-Pix 990. If only I could remember 10% of
} its function modes and how to access them.
}
} Bart Cannon
} Cannon Microprobe
} Seattle
}
}


From daemon Thu Oct 5 11:34:58 2000



From: Daniel Ross :      DKRoss-at-uh.edu
Date: Thu, 05 Oct 2000 11:35:00 -0500
Subject: Ge vs Si detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


We have a Ge detector and get considerably better resolution with it than we
get with an older SiLi detector. The Ge detector has an ultrathin window,
the Si detector has a Be window. They are both used for x-ray detection in
the 0-20 KeV range.

Kent Ross

-----------------------------------------
Kent Ross
Research Associate
Texas Center for Superconductivity
University of Houston
77204-5932
DKROSS-at-uh.edu
713-743-8284
------------------------------------------



From daemon Thu Oct 5 11:44:13 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 5 Oct 2000 09:31:26 -0700
Subject: RE: Coolpix 990 to Axioskop (or any other photomicroscope) "re-sent"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


PETER writes ...

} mounting the Coolpix 990 to any photomicroscope is absolutely
} simple via the usual C-mount connection. For the coolpix you have
} to buy at NIKON the microscope adapter ...
} The fantastic thing is that you can focus not only via the LCD-
} screen of the camera ( possible, but not so easy in my opinion) but
} via an external TV-Monitor in live modus, yielding really smashing
} results with a resolution satisfying publication needs.
} With my Coolpix sometimes the camera "hangs up" when the Auto-
} power off mode is activated (de-activating it helps). Does anybody
} have this problem as well ?? ...

The AC adapter should allow for the video output to remain on ...
leastwise, that is how my CP800 works.

} What is lacking for the Coolpix 990 at the moment is a cable
} release, ...
}
} All in all, the Coolpix is the first digital camera under
} 5000 US-$ ...

The 990 is indeed feature rich, and the cable release will be needed
for microscopy (... altho you should be able to acquire with a steady
camera if you take a picture with the time-delay feature ...).

I have realized just lately, altho the CP950 and 990 are the
feature-rich and most desireable, the CP800 will work (altho somewhat
awkwardly) ... providing the same NTSC/PAL out and lens threads. If
you find a lesser expensive 1x C-mount adapter (as available at Optem
International {www.optemintl.com} ), then you have a digital camera
capable of 1600by1200 for approximately US$800. You should figure an
additional $100 for AC adapter ($50) and several NiMH batteries and
charger (~$50).

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Thu Oct 5 12:15:15 2000



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Thu, 5 Oct 2000 13:10:21 -0400 (EDT)
Subject: Re: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Robin,

Have you considered or looked into the Sony 390P color video camera?? It
gives you a live image with an RGB output, C-mount and 1.3 million pixels
(effective resolution) and sells for just under $ 3,000.

If you would like any more information, please let me know.

Sincerely,

Gary

Gary Liechty
Manager, Technical Products

Allied High Tech Products, Inc.
2376 E. Pacifica Place
Rancho Dominguez, CA 90220

310-635-2466
800-675-1118 US and Canada
310-762-6808 Fax

Products for Metallurgical Sample Preparation

www.alliedhightech.com
----- Original Message -----
} From: {"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, October 05, 2000 5:54 AM


Simon,

I suspect that diatomaceous earth will have mostly fragments in it. If you
let me know your address, I'll try to find an old diatom SEM preparation to
send you and I'll also ask some folks here for a fresh sample of whole
diatoms that you can prepare for viewing yourself.

Gary,

Being a microscopist, not a microbiologist/paleontologist/sedimentologist
(though I've imaged my share of microplankton for them) I can only give a
brief overview of the differences between diatoms and radiolaria, but here
it is. Rads are floating, single celled marine protozoa that live inside
ornate opaline tests (external shells) that look like little Christmas tree
ornaments or something you might play badminton with. Their shells have
lots of holes, spikes and spines and can be spherical, amorphously
latticed, rather bulbous with long spikes from one end, sub-triangular,
etc. The cell body inside sends out numerous fine cytoplasmic strands that
radiate outward to form a sticky halo that captures food. Diatoms are
single-celled plants that live wherever there's moisture - salt water,
fresh water, soil, even on whale skin. They can be planktonic
(free-floating) or benthic (attached to a substrate) or live on mud or
rocks. Some live in the dark, but most need light to photosynthesize.
Their siliceous tests are made of two valves that fit together like a pill
box and come in two basic shapes, round and pennate. (The pennate ones can
look like peanuts or canoes.) Diatom tests are also quite ornamental, with
holes, radial designs, or long, sometimes feathery, projections, and
sometimes they link together to form long chains. There's also a great
variety in size among species.

Dee


***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Thu Oct 5 12:53:38 2000



From: Gary Radice :      gradice-at-richmond.edu
Date: Thu, 5 Oct 2000 13:47:32 -0400
Subject: re:CoolPix 990 to Axioscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


You might also try contacting Bunton Instruments in the US:

{http://www.buntgrp.com/}

They sell microscope adaptors for the CoolPix. The adaptors are not listed
on their web site, yet, but call or e-mail them for information. I know
they are taking orders for adaptors now and I was told that they would be
shipping "around the end of October."

I have no connection with Bunton Instruments other than as a customer.

Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice




From daemon Thu Oct 5 12:54:03 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 05 Oct 2000 13:48:09 -0400
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Brian-

You don't mention at what magnifications you see the sawtooth. However, if
you see it in the alphanumerics, I can't see that it can be anything other
than a problem in your display unit - a 60Hz interference in the line scan
drive (but not the scan generator) or something like that. If that is the
case, the magnification wouldn't make any difference.

Good luck,

Tony Garratt-Reed

} hi all-
}
} i've installed a 35 and have some issues with a saw-tooth distortion in the
} scan. it stays constant regardless of KV, but the number of saw-tooths
} (teeth?) in a frame increases with slower scan rates. i suspected
} vibration so i mounted the roughing pumps on a big foam block.....no
} change. i also cycled off the chiller unit with no change, so its probably
} not mechanical vibrations, unless there is another vibration causing
} component i'm overlooking.
}
} the distortion is also visible in the data field alphanumerics (they shift
} and shimmy a little) with the beam turned off, so i don't suspect a HV
} instability problem.
}
} can i logically deduce that there is an electrical field leakage that is
} messing up the scan? or a ground loop? what about cable placement? or a
} problem with the 100V variac (its within 3' of the column).
}
} right now the column is a bit dirty and needs cleaning....i've never seen,
} for example, dirty apertures cause this type of distortion though.
}
} any suggestions would be appreciated!
}
} brian
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875
}

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Thu Oct 5 13:25:18 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 05 Oct 2000 13:30:28 -0500
Subject: Re: Ge vs Si detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


We currently have 4 Ge EDX detectors and 1 Si(Li) detector, all from Oxford
Instruments. The Ge detectors do have a lower background than the Si(Li) and
the resolution is better. The Ge detectors range from 109 ev to 115 ev
whereas the Si(Li) is around 132 ev. We routinely use all of them for low-kV
work. The Ge detectors do a better job because of the resolution and the
lower background. I do SEM of semiconductors, so 30kV is as high as I can
go. I can't comment on the Ge detector's performance at higher than that, but
they are my favorite detector in the 0.5 kV to 30kV range.
N.B.: I have no financial interest in Oxford Instruments; I'm just a
satisfied customer.

Chris Jeffree wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} High-purity Germanium detectors provide *better* resolution than
} Si(Li) detectors, and also have lower low-keV background. The
} higher atomic number of Ge means they have greater x-ray
} stopping power and therefore higher detection efficiency for the K
} lines of the heavier elements, and they have therefore been thought
} of as primarily useful at higher beam accelerating voltages for
} heavy element analysis. However, this may only be one of their
} advantages. The better resolution and lower background they offer
} at low keV suggests they would also be good, and perhaps
} preferable to Si for light element detection and peak discrimination
} which could mean more options for low-kV excitation in Field
} emission SEMs.
}
} What are the flaws in this argument?
} It would be interesting to hear comments on the above from anyone
} who has direct knowledge and experience of both types of
} detectors.
}
} BTW besides PGT, both Gresham and Oxford sell Ge detectors. I
} am not aware of any others
} Chris
}
} } From: "NVo-at-IGC.com"-at-sparc5.microscopy.com
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ge vs Si detector
} Date sent: Wed, 4 Oct 2000 13:15:58 -0400
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listners:
} }
} } Any comments on which would make a better detector, Ge or Si ? only PGT
} } sells Ge detectors and I'm aware of the decrease in resolution with Ge
} } detector. Could someone lists or details the benefits of having a Ge
} } detector as oppose to a Si one.
} }
} } Sincerely
} }
} } N.V.Vo
} }
} }
} }
} } N.V.Vo, Ph.D.
} } Intermagnetics General Corporation
} } 450 Duane Avenue
} } Schenectady, New York 12304
} } Phone: 518 346 1414 x 3107
} } Fax: 518 346 6080
} } E-mail: nvo-at-igc.com
} } www.igc.com
} }
} }
}
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 (0) 131 650 5345
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Oct 5 14:11:59 2000



From: SMancuso-at-specialmetals.com
Date: Thu, 5 Oct 2000 15:08:07 -0400
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Brian,

Our 35C has several times displayed that saw-tooth interference in the
past.
Each time it was attributed to some ground loop problem. One place to look
is in the ribbon aperture. Make sure that the insulators between the
aperture
strip and holder are properly set or 'not missing'.

I agree that the distortion is not from HV instability which usually
displays some
darkness or brightness irregularity.

Good luck!
_
/ \ Sam O. Mancuso
/\ /\ Special Metals Corporation
(__n__) New Hartford, NY





Brian McIntyre
{mcintyre-at-optics.roch To: Microscopy-at-sparc5.microscopy.com
ester.edu} cc:
Subject: saw-tooth scan distortion: jeol35
10/05/2000 10:04 AM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


hi all-

i've installed a 35 and have some issues with a saw-tooth distortion in the
scan. it stays constant regardless of KV, but the number of saw-tooths
(teeth?) in a frame increases with slower scan rates. i suspected
vibration so i mounted the roughing pumps on a big foam block.....no
change. i also cycled off the chiller unit with no change, so its probably
not mechanical vibrations, unless there is another vibration causing
component i'm overlooking.

the distortion is also visible in the data field alphanumerics (they shift
and shimmy a little) with the beam turned off, so i don't suspect a HV
instability problem.

can i logically deduce that there is an electrical field leakage that is
messing up the scan? or a ground loop? what about cable placement? or a
problem with the 100V variac (its within 3' of the column).

right now the column is a bit dirty and needs cleaning....i've never seen,
for example, dirty apertures cause this type of distortion though.

any suggestions would be appreciated!

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875







From daemon Thu Oct 5 15:02:09 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Thu, 5 Oct 2000 12:54:53 -0700
Subject: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Carolina Biological Supply has diatoms for this purpose.
I just take them out of the jar with a dropper and make a wet mount with a
coverslip.

You can get them live or preserved.

www.carolina.com

Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu


-----Original Message-----
} From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com
[mailto:"s_woodland-at-madasafish.com"-at-sparc5.microscopy.com]
Sent: Wednesday, October 04, 2000 8:05 AM
To: Microscopy-at-sparc5.microscopy.com


---------------------------------------------------------------------------

Email: s_woodland-at-madasafish.com
Name: Simon Woodland
School: Kaskenmoor School

Question: Where can I get some diatoms to put under our microscope? How
should they be prepared and mounted.

---------------------------------------------------------------------------




From daemon Thu Oct 5 15:29:43 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 6 Oct 2000 09:29:50 GMT+1200
Subject: Re: SEM Help needed on conductive adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



}
} I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with
} Electrodag 502 for secondary electron imaging. The adhesive is a graphite
} paint in MEK base (a substitute for TV Tube Koat). After ethanol
} dehydration and CP drying in liquid CO2, the samples are relatively flat or
} slightly curved. However, once attached to the stub the skins develop a
} noticeable curl which leaves a gap between the stub surface and sample. In
} some cases I can see the "trail" in the adhesive left by the skin as it was
} curling! Filling the gap with more Electrodag is a waste of time because
} it evaporates and shrinks back away from the sample (thankfully it doesn't
} seem to produce more curling).
}
} Could anyone suggest a better conductive adhesive? Also, is there any
} difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based
} media as to its effect on biological samples?
}


I have found the conductive double-sided adhesive stuff available
from several EM suppliers in both disc and tape form to be pretty
good.
But maybe you can't press your samples down onto it.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Oct 5 15:36:13 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 6 Oct 2000 09:35:36 GMT+1200
Subject: Re: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html





} I was told one lab installed a switch to turn the
} SEM PC cooling fans off for short periods of time during high resolution
} work. That is a risky fix if you are over 50 years old.

??????????????????????????????

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Oct 5 16:21:30 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 5 Oct 2000 17:14:02 -0400
Subject: Re: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



}
}
} } I was told one lab installed a switch to turn the
} } SEM PC cooling fans off for short periods of time during high resolution
} } work. That is a risky fix if you are over 50 years old.
}
} ??????????????????????????????

Clarification:
I just turned 50. I if I attempted to turned these fans off manually for "a
few minutes", chances are the PC processor would shut down on overheat
before I remembered to turn them back on. It's an 'out of memory' problem.
Jim



}
} rtch
}
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Thu Oct 5 16:47:31 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, October 05, 2000 10:20 AM
Subject: Re: Ge vs Si detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


EG&G ORTEC used to manufacture Germanium EDS detectors too. I do not know
whether they still do it since they had become part of Perkin-Elmer. They
have, however, a service group which does service Germanium detectors.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk}
To: "NVo-at-IGC.com"-at-sparc5.microscopy.com
{"NVo-at-IGC.com"-at-sparc5.microscopy.com}
Cc: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}



From daemon Thu Oct 5 16:47:31 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, October 05, 2000 11:51 AM
Subject: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Brian,

Information in your posting strongly suggests ground loop(s) and possibly EM
(electromagnetic) fields in the room too. It is possible for local EM
interference to affect signal cables without affecting the SEM column much.
Did you try EM shielding with sheets of soft Iron? If that makes no
difference (it probably wouldn't, since HT set makes no difference for the
distortion in your case), than some work needs to be done with
shielding/grounding of the various units/components of the SEM. Let us say
here, one who has the experience of eliminating 60Hz noise in audio circuits
can do the job.
Another possibility- did you check DC power supply voltages in your SEM for
60Hz noise? It must be filtered out well.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Brian McIntyre {mcintyre-at-optics.rochester.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Thu Oct 5 16:48:47 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thu, 5 Oct 2000 17:33:12 -0400
Subject: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thank you for all the responses - exactly what we looking for. MSA
listserver is at great help, as usual.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.



From daemon Thu Oct 5 17:55:01 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Thu, 5 Oct 2000 17:48:00 -0600 (=?ISO-8859-1?Q?Hora_est=E1ndar_de_M=E9xico?=)
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Brian,

your SEM interference problem sounds almost the same as ours that I not
long ago put on the listserver - the sawtooths in the scans. Sorry I
haven't got an answer to the problem, but I'd be very interested if you
manage to solve it! We are going to do a trial with mu-metal in the near
future shielding the column of our SEM (Jeol 5410-LV) so I'll let you know
how it goes. We suspect electromagnetic field interference.

Good luck,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************




From daemon Thu Oct 5 18:15:06 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 05 Oct 2000 16:04:42 -0700
Subject: Re: Digital microscope camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Robin,
For your application, you may find the Pixera camera to be a cost-effective
solution. At the same resolutionoas your current camera, it costs about $1100.
At 07:54 AM 10/5/00 -0500, you wrote:

} } I'm looking to replace my two year old Polaroid Digital Microscope camera
} } (SCSI interface with color or black and white, 1600x1200 pixels). The
} } chip has failed and I don't want to buy another Polaroid camera. I do
} } primarily black and white photography from a Zeiss optical microscope for
} } materials engineering applications. I need for the camera to save in tiff
} } format without changing the dimensions of the objects. I don't need low
} } light applications.
} }
} } Any suggestions?
} }
} } Thanks,
} }
} } Robin Griffin
} }
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Oct 5 18:27:41 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 05 Oct 2000 16:17:54 -0700
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Brian,
You may have ripple on another power supply, such as lens or scan coils. I
always try to put the variac as far from the column as possible. Check for
something loose on the column or stage: I've had that effect when the gun HV
supply cable was not tied down. Make sure there is a good vibration break
(heavy weight) in the foreline hose. The service engineer at ours turned off
the rotary pump for a few seconds, to see if it changed. Another source of
vibration was a belt-drive pump in another room, but on the same floor beam.
A dirty column won't cause this. It may be building vibration.
At 10:04 AM 10/5/00 -0400, you wrote:
}
} hi all-
}
} i've installed a 35 and have some issues with a saw-tooth distortion in the
} scan. it stays constant regardless of KV, but the number of saw-tooths
} (teeth?) in a frame increases with slower scan rates. i suspected
} vibration so i mounted the roughing pumps on a big foam block.....no
} change. i also cycled off the chiller unit with no change, so its probably
} not mechanical vibrations, unless there is another vibration causing
} component i'm overlooking.
}
} the distortion is also visible in the data field alphanumerics (they shift
} and shimmy a little) with the beam turned off, so i don't suspect a HV
} instability problem.
}
} can i logically deduce that there is an electrical field leakage that is
} messing up the scan? or a ground loop? what about cable placement? or a
} problem with the 100V variac (its within 3' of the column).
}
} right now the column is a bit dirty and needs cleaning....i've never seen,
} for example, dirty apertures cause this type of distortion though.
}
} any suggestions would be appreciated!
}
} brian
Good luck!

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Oct 5 18:42:30 2000



From: Al Coritz :      al-at-boeckeler.com
Date: Thu, 5 Oct 2000 16:27:19 -0700
Subject: Ventana-RMC EM Products

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


October 6 , 2000


Dear Valued Customer,


We are pleased to inform you that Boeckeler Instruments, Inc., a local
Tucson high technology company, has acquired the RMC Electron Microscopy
product line from Ventana Medical Systems.

The RMC product management group including Dave Roberts, Greg Becker, Al
Coritz and Gareth Morgan will stay with the RMC product line and continue to
give you excellent support under the ownership of Boeckeler Instruments.

During the next few days we will be in the process of moving offices,
production lines and inventory so please accept our apologies if you
experience any difficulties or delays during this brief transitional period.
We will do our utmost to minimize any inconvenience to you by making this
move as “transparent” as possible to the outside world and we appreciate
your patience & continued support as we relocate the business.
Since 1942 Boeckeler has been and still is the industry leader in
manufacturing high quality and accurate micrometer and positioner heads.
Boeckeler Instruments also manufactures a complete line of video
measuring, cross hair and marking products all of which are very
complementary to the RMC ultramicrotomes and EM sample preparation
instruments.

We look forward to a long and continuing relationship with you as our highly
valued customer and hope to be of service to you in the very near future.

If you have any questions or concerns please contact:


Boeckeler Instruments, Inc.

4650 South Butterfield Drive

Tucson, AZ 85714


Tel: (520) 745-0001 Fax: (520) 745-0004

email: info-at-boeckeler.com


Sincerely


Chris Gleeson
President & Chief Executive Officer
Ventana Medical Systems, Inc.



From daemon Thu Oct 5 19:07:13 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 5 Oct 2000 17:53:35 -0600
Subject: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Brian,

what you see could be 60-Hz Noise. It follows the characteristics you are
describing (more 'teeth' with lower scan rate). Can you do a
back-of-the-envelope calculation of how many 'teeth' you get per second
(check exposure settings and line times). If it's around 60, you should try
to minimize the influence of 60 Hz Noise (ground loops, check all grounding
contacts, etc.)

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Brian McIntyre [mailto:mcintyre-at-optics.rochester.edu]
Sent: Thursday, October 05, 2000 8:04 AM
To: Microscopy-at-sparc5.microscopy.com


hi all-

i've installed a 35 and have some issues with a saw-tooth distortion in the
scan. it stays constant regardless of KV, but the number of saw-tooths
(teeth?) in a frame increases with slower scan rates. i suspected
vibration so i mounted the roughing pumps on a big foam block.....no
change. i also cycled off the chiller unit with no change, so its probably
not mechanical vibrations, unless there is another vibration causing
component i'm overlooking.

the distortion is also visible in the data field alphanumerics (they shift
and shimmy a little) with the beam turned off, so i don't suspect a HV
instability problem.

can i logically deduce that there is an electrical field leakage that is
messing up the scan? or a ground loop? what about cable placement? or a
problem with the 100V variac (its within 3' of the column).

right now the column is a bit dirty and needs cleaning....i've never seen,
for example, dirty apertures cause this type of distortion though.

any suggestions would be appreciated!

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875


From daemon Thu Oct 5 19:35:24 2000



From: Pierre Hovington :      hovington.pierre-at-ireq.ca
Date: Thu, 5 Oct 2000 19:27:18 -0500
Subject: Annoncment for a FESEM workshop in Montreal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




ANNOUNCEMENT
FE-SEM COURSE


It is with great pleasure that I am announcing our 2001 FE-SEM and Image
Analysis Courses

When: May 21-25, 2001

Guest Speaker: Dr. David C. Joy
Professor, Tennessee University
Marin Lagace,
Researcher, Hydro-Quebec Research Center

Where: IREQ (Hydro-Quebec Research Centre),
Varennes(Montreal), QC.,
Canada

Topics Covered: FE-SEM Components: gun, lenses, apertures, vacuum
technology...
Care & feeding of the FEG
The secrets of successful FE-Microscopy
Vacuum hygiene, cleaning and storing samples
Unwanted Beam Interactions
What is needed for High Resolution SEM
Image Content
Getting the most from your SEM
How to assess resolution
Low Voltage Work - High Resolution at low energy

LVSEM and charging
FESEM and Microanalysis
Low Energy EDS Work
plus so much more

This 3-day workshop will be devided in Lecture and
Practical Sessions

Image Analysis : Numerical filters description
Uses of mathematical morphology operators (erosion,
dilatation, etc)
Data analysis and interpretation
Recipes for common Image Analysis tasks


Costs: $1,500.00 (Can$) for FE-SEM and Image Analysis Workshops

$ 250.00 (Can$) for the Image Analysis Workshop ONLY


For more information (and/or for reservation) please contact:

Dr Pierre Hovington
tel: 450 652-8125
fax: 450 652-8905
e-mail: hovington.pierre-at-ireq.ca




From daemon Thu Oct 5 19:39:09 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 5 Oct 2000 19:31:56 -0500
Subject: SEM Help needed on conductive adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


What about using Superglue? I routinely use it when
I need a good adhesion of samples. You really do not
need a conductive adhesive with a sample thickness of
about 3 mm - it's better just to draw a line with carbon
adhesive from the edge of a sample to a stud prior
conductive coating.

Vladimir Dusevich


-----Original Message-----
} From: "carla_aiwohi-at-usgs.gov"-at-sparc5.microscopy.com
To: Microscopy-at-sparc5.microscopy.com
Sent: 10/5/00 8:46 AM


Hello all,

I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs
with
Electrodag 502 for secondary electron imaging. The adhesive is a
graphite
paint in MEK base (a substitute for TV Tube Koat). After ethanol
dehydration and CP drying in liquid CO2, the samples are relatively flat
or
slightly curved. However, once attached to the stub the skins develop a
noticeable curl which leaves a p between the stub surface and sample.
In
some cases I can see the "trail" in the adhesive left by the skin as it
was
curling! Filling the gap with more Electrodag is a waste of time
because
it evaporates and shrinks back away from the sample (thankfully it
doesn't
seem to produce more curling).

Could anyone suggest a better conductive adhesive? Also, is there any
difference between aqueous (eg. Aquadag colloidal graphite) and
MEK-based
media as to its effect on biological samples?

As an SEM novice any advice you could share with me is greatly
appreciated.
Thank you very much,

Carla Aiwohi
Western Fisheries Research Center
Seattle, WA
ph: (206) 526-6282 ext.242
fax: (206) 526-6654



From daemon Thu Oct 5 19:55:48 2000



From: andrewb-at-vsl.cua.edu
Date: Thu, 5 Oct 2000 20:48:28 -0400
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


You wrote:

} i've installed a 35 and have some issues with a saw-tooth distortion in
} the scan.

My 35C had an aperture heater which used to go into oscillation
and produce the kind of problem you describe. It has long since
been disconnected. It doesn't seem likely in your case because
you see the problem with the beam off, but I thought it was worth
mentioning just in case.


Sincerely yours,
Andy Buechele

Andrew C. Buechele, Ph.D.
Research Professor
Vitreous State Laboratory
The Catholic University of America
409 Hannan Hall
Washington, D.C. 20064
(202) 319-4995 FAX: (202) 319-4469


From daemon Thu Oct 5 20:55:35 2000



From: Nancy_Robertson :      Nancy_Robertson-at-dnr.state.ak.us
Date: Thu, 05 Oct 2000 17:51:19 -0800
Subject: Carbon Coated Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I need good quality carbon coated grids for viewing purified plant virus
particles with TEM. I have had little success with "store bought" coated
grids. So unless someone could recommend a good cc grid source, I will
need to buy a carbon coater. Should I purchase a carbon coater using
flash evaporation or the more expensive carbon turbo evaporator?
In addition, could anyone recommend an economically good microtome for
thin sectioning plastic embedded specimens?

Thank You,

Nancy L. Robertson, Research Plant Pathologist
USDA/ARS
National Arctic Plant Germplasm Resources Unit
533 Fireweed Ave.
Palmer, Alaska 99645
phone: 907-746-9465
fax:907-746-2677
e-mail: nancy_robertson-at-dnr.state.ak.us


From daemon Fri Oct 6 01:57:22 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 6 Oct 2000 09:10:04 +0100
Subject: Ge vs Si detectors - what are the shortcomings of Ge?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Another way to pick up noise and voltage is to wires wrapped around each
other. It is east to test just watch the screen and poke the wires with a
wood stick. If there is any problem in the wiring it will show up on the
screen. You only have to move them gently.

A wooden stick and light tapping can find a lot of problems. If you are
working in the high voltage section make sure it is a long dry stick. It
is great for finding intermittent problems. Just start tapping until the
problem appears or goes away and then narrow in on the problem.

When you check the frequency if it comes up 120 Hz florescent lights
become the number 1 suspect. You could be picking up electrical noise from
them or you have something photo
sensitive somewhere. Any silicone junction is sensitive to light. The
quick test is to turn off the lights. If they are the problem it should at
least get weaker.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

----- Original Message -----
} From: "Mike Bode" {mb-at-Soft-Imaging.com}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, October 05, 2000 6:53 PM


Thank you all for your replies on this topic. Several people seem to
like Ge detectors for low keV work, and the replies have mostly been
very positive about their resolution and performance. Some authors
strongly advocate the use of Ge detectors for low kV, low probe
current work with light element samples (See, for example, Block-
van Hoek & Pinxter (1993) Chapter 1 in X-ray microanalysis in
biology edited by Sigee et al, Cambridge University Press). Their
opinion (and apparently yours) stands in contrast with the advice
given by all of the XRMA vendors I am speaking to, whose opinion is
that Si(Li) is the way to go (granted some of them don't sell Ge, so
they would obviously have to advocate Si). So my question is "what
is the Achilles heel of Ge detectors?". I am aware for example that
their potentially very favourable characteristics at low kV are spoiled
by the practical difficulty of manufacturing them without a large dead
layer. Is this a fatal flaw in practice for analysis of elements with z
{11? Are they less robust, less tolerant of thermal cycling? If
anyone can help me get to the bottom of this I would be most
grateful.

Chris

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Fri Oct 6 03:44:22 2000



From: Alex :      tikhonov-at-mpi-halle.mpg.de
Date: Fri, 6 Oct 2000 10:36:19 MET
Subject: grinding plate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I am grinding a TEM specimen in a Multipol polishing machine, but a
grinding plate is covered with some stains of grayish color whose
origin I cannot identify. I tried to apply ethanol, atheton, and methanol
to them, but these proved in vain.

Please suggest any cleaning liquid to wipe the grinding plate clean.

Alex


Aleksandr Tikhonovsky, Ph.D.
tikhonov-at-mpi-halle.mpg.de
tel 49 345 5582 929
Max-Planck-Institut für Mikrostrukturphysik
Weinberg 2
D-06120 Halle
Germany


From daemon Fri Oct 6 03:59:49 2000



From: Allen R. Sampson :      ars-at-sem.com
Date: Fri, 6 Oct 2000 03:52:04 -0500
Subject: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


You've had a lot of replies, but your symptoms are clear. In most cases,
this interference is caused by an electrical device mounted too close to
the SEM CRT - An EDS monitor, vacuum gauge controller, etc. If not, you
probably have a bad filter or bypass capacitor in a power supply that the
CRT uses. Since the effect is also seen in the alpha-numerics, it is
directly affecting the view CRT itself. Keeping the area around the CRT
clear of other instruments and checking for ripple in the power supplies to
the CRT should solve the problem. The effects of external instruments near
the view CRT are generally very local and movement of just a few inches can
make a huge difference.

It is not mechanical vibrations and the CRTs are not that suseptible to
electro-magnetic interference from distant sources.

If the alpha-numerics were not affected, I would suggest checking the
relative difference in the noise when using a low and high working
distance. If there is a noticably greater noise when at a larger working
distance, then electro-magnetic fields affecting the beam would be suspect.
The column of an SEM is actually well shielded from EM effects, as the
lenses are encased in large ferro-magnetic structures to provide the
focussing of the lens effects to the pole pieces. If lower working
distances are used (sample closer to the final pole piece) then the effects
of EM are reduced since they are primarily induced through the sample
chamber and affect the beam from the final pole piece to the sample
surface.

However, as I've said, if the alpha-numeric display is also affected, then
more than likely the CRT beam is being affected by something close by or by
a power supply to the CRT. The high voltage to the CRT (around 10KV) is
not a likely suspect as the record CRT is much more suseptable to HV
problems and in the JSM-35, as in most SEMs, is fed from the same supply.
You would have seen some obvious focus problems in your micrographs if the
high voltage was a problem. As the view CRT is an integral part of the
electronics console, ground loop problems are unlikely.

The record CRT and view CRT are located close together in this instrument
and as I recall they use the same power supplies. The record CRT is
probably recording a problem as well, but it may be greatly reduced. The
record cycle uses a greatly reduced scan speed, so these variations in the
view CRT are going to show up as reduced edge sharpness in your micrographs
or not at all if each record scan line is triggered by a zero crossing on
the 60Hz input (I don't recall if the JSM-35 does this).


Allen R. Sampson, Owner
Advanced Research Systems, St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092

-----Original Message-----
} From: Brian McIntyre [SMTP:mcintyre-at-optics.rochester.edu]
Sent: Thursday, October 05, 2000 9:04 AM
To: Microscopy-at-sparc5.microscopy.com


hi all-

i've installed a 35 and have some issues with a saw-tooth distortion in the
scan. it stays constant regardless of KV, but the number of saw-tooths
(teeth?) in a frame increases with slower scan rates. i suspected
vibration so i mounted the roughing pumps on a big foam block.....no
change. i also cycled off the chiller unit with no change, so its probably
not mechanical vibrations, unless there is another vibration causing
component i'm overlooking.

the distortion is also visible in the data field alphanumerics (they shift
and shimmy a little) with the beam turned off, so i don't suspect a HV
instability problem.

can i logically deduce that there is an electrical field leakage that is
messing up the scan? or a ground loop? what about cable placement? or a
problem with the 100V variac (its within 3' of the column).

right now the column is a bit dirty and needs cleaning....i've never seen,
for example, dirty apertures cause this type of distortion though.

any suggestions would be appreciated!

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875




From daemon Fri Oct 6 05:03:39 2000



From: Allen R. Sampson :      ars-at-sem.com
Date: Fri, 6 Oct 2000 04:48:57 -0500
Subject: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Have an EDS system attached? The EDS dewars, being generally supported by
a cantilever structure, can be quite effective at coupling acoustic
vibrations into an SEM. The large area and thin metal construction of the
dewar as well as the large momentum arm of the cantilever provide for an
amplification of acoustics into the instrument. You might also try to
provide some form of isolated support and damping of the EDS system.

The size, shape and construction of the room can also have a considerable
effect. Certain configurations can amplify acoustic and construction
material mediated effects. Placement of the SEM within a particular room
can make a big difference. Particularly bad is the placement in an alcove
or bay of a room. Walls close to the sides of an instrument produce
complex acoustic patterns. Each wall surface represents a resonator that
can vibrate with unexpected sources. An instrument I had placed in this
situation in a clean room of a major alphabet soup named manufacturer had
incredible sensitivity to door closings and even footsteps in the adjoining
airlock and preparation areas.

Your mention of 5 jaggies begs the question - at what scan rate were these
viewed? What is the time span for each full scan? The SEM is an
incredibly sensitive seismometer. I've seen the effects of waves crashing
on the shores of an island as well as the vibrations from an M1-A1 Abrams
tank at 40 miles per hour on a nearby proving ground. It can often be
difficult to isolate the acoustic effects from the mechanically induced
effects (those coupled through solid materials). Most SEMs provide good
isolation from high frequency mechanical coupling, but low frequencies are
problematic.

Vibrational problems, unlike electro-magnetic problems, affect the beam
along it's entire path to some degree. In the column, most of these
effects are canceled by the fact that a movement that causes the beam to be
deflected outside of it's normal path will be countered by the increased
magnetic effect of the lenses as the beam goes off-axis. The greatest
effect though, is in the path from the final pole piece to the sample
surface. Your determination of the effect being the same from 4mm to 10mm
would probably not be true if you measured the difference from 4mm to 25mm.



Allen R. Sampson, Owner
Advanced Research Systems, St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092

-----Original Message-----
} From: Ben Craft [SMTP:bcraft-at-uci.edu]
Sent: Wednesday, October 04, 2000 9:52 PM
To: Microscopy-at-sparc5.microscopy.com


I'm trying to solve a high resolution noise problem with a XL-30
Phillips FESEM. The room that it is in was recently checked and was ok,
accept for some acoustic noise. To fix the noise I put up 3 inch sound
proofing foam and quieter ducting for the air condition. The noise
showed up in an image by a streak about 5 jaggies. After
putting up the foam the noise was reduced, but it is still there. The
noise is the same for 4mm working distance or a 10mm working distance.

Any one had a similar problem or have any advice?

Thanks,
Ben




#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \







From daemon Fri Oct 6 07:00:38 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 06 Oct 2000 07:39:49 -0700
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Brian McIntyre wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} hi all-
}
} i've installed a 35 and have some issues with a saw-tooth distortion in the
} scan. it stays constant regardless of KV, but the number of saw-tooths
} (teeth?) in a frame increases with slower scan rates. i suspected
} vibration so i mounted the roughing pumps on a big foam block.....no
} change. i also cycled off the chiller unit with no change, so its probably
} not mechanical vibrations, unless there is another vibration causing
} component i'm overlooking.
}
} the distortion is also visible in the data field alphanumerics (they shift
} and shimmy a little) with the beam turned off, so i don't suspect a HV
} instability problem.
}
} can i logically deduce that there is an electrical field leakage that is
} messing up the scan? or a ground loop? what about cable placement? or a
} problem with the 100V variac (its within 3' of the column).
}
} right now the column is a bit dirty and needs cleaning....i've never seen,
} for example, dirty apertures cause this type of distortion though.
}
} any suggestions would be appreciated!
}
} brian
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875
Brian,
You didn't say whether or not the sawtooth shows up on micrographs. If
the problem were 60 Hz, they would not be apparent because the record
scan speeds are sync'd to the line frequency. If you DO see them on
micrographs, start by looking at your low voltage supplies, the CRT HV
supply and the scan generator signals.

The problem has nothing to do with your column, since the characters
show the problem. CRT HV instabilities should show up more the further
from the center of the CRT you get. You should also be getting both
light and dark lines as the vertical, as well as the horizontal, will be
affected.

If you don't see the above symptoms, look at both scan signals going to
the CRT. You may see noise on the horizontal signal, but if the
sawtooth is showing up in the image, also, you probably won't see the
noise on the horizontal signal to the column, because they aren't
syncronized. If they both had the same noise, the image would look fine
even though the alphanumerics shimmied.

Because of the alphanumerics, there is some processing of the CRT scan
signals after the scan generator that does not happen to the scan signal
sent to the mag control/column. A good place to look.

If the problem only occurs on 1 CRT, look at its drivers.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA


From daemon Fri Oct 6 07:40:52 2000



From: LungoFam-at-gte.net
Date: Fri, 6 Oct 2000 07:37:13 -0500
Subject: Ask-A-Microscopist: IgG question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Email: LungoFam-at-gte.net
Name: William Lungo
School: East Los Angeles College
State: California

Question: Protocol for processing biological specimens for IgG immuno gold
using LR White embedding media, for electron Microscopy

---------------------------------------------------------------------------




From daemon Fri Oct 6 08:17:09 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 06 Oct 2000 09:12:05 -0400
Subject: Re: Carbon Coated Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Nancy,

If you have not tried our carbon coated grids, we believe we make pretty
good ones. If you have tried ours, please let me know what you didn't
like and we can correct it since we make them up fresh for each order,
and can make them thinner, thicker or whatever you wish.
In a worst case scenario, we make what we consider the best evaporator
in the business.

John Arnott

--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Microscope Supplies Since 1955

Nancy_Robertson wrote:
}

}
} Hi,
}
} I need good quality carbon coated grids for viewing purified plant virus
} particles with TEM. I have had little success with "store bought" coated
} grids. So unless someone could recommend a good cc grid source, I will
} need to buy a carbon coater. Should I purchase a carbon coater using
} flash evaporation or the more expensive carbon turbo evaporator?
} In addition, could anyone recommend an economically good microtome for
} thin sectioning plastic embedded specimens?
}
} Thank You,
}
} Nancy L. Robertson, Research Plant Pathologist
} USDA/ARS
} National Arctic Plant Germplasm Resources Unit
} 533 Fireweed Ave.
} Palmer, Alaska 99645
} phone: 907-746-9465
} fax:907-746-2677
} e-mail: nancy_robertson-at-dnr.state.ak.us


From daemon Fri Oct 6 08:53:42 2000



From: jshields-at-cb.uga.edu
Date: Fri, 6 Oct 2000 09:53:06 -0400
Subject: refraction index question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


------- Forwarded message follows -------
} From: Charles Keith {chkeith-at-cb.uga.edu}
To: farmer-at-emlab.cb.uga.edu
Date sent: Thu, 5 Oct 2000 09:47:00 -0400 (Eastern Daylight
Time) Priority: NORMAL

Mark -

Could you post a request to your microscopy listserv for
the index of refraction of Dulbecco-Vogt PBS? I have done
so to sci.techniques.microscopy

----------------------
Charles Keith
Associate Professor, Department of Cellular Biology
(http://www.uga.edu/~cellbio)
University of Georgia
chkeith-at-cb.uga.edu

John Shields
EM Lab
151 Barrow Hall
UGA campus
2-4080
fax: 2-4271
jshields-at-cb.uga.edu


From daemon Fri Oct 6 08:54:47 2000



From: Julie Piraino :      piraino-at-sms.si.edu
Date: Fri, 06 Oct 2000 09:52:34 -0400
Subject: re:CoolPix 990 to Axioscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Martin Microscope Company of Easley, SC, USA makes an adapter for the Coolpix 990 to fit the eyepiece tube of any microscope (both 23mm and 30mm diameter tubes) for $300. They can also get you an adapter for C-mount. Their phone number is 864-242-3424 or 864-859-2688

} } } Gary Radice {gradice-at-richmond.edu} 10/05/00 01:47PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


You might also try contacting Bunton Instruments in the US:

{http://www.buntgrp.com/}

They sell microscope adaptors for the CoolPix. The adaptors are not listed
on their web site, yet, but call or e-mail them for information. I know
they are taking orders for adaptors now and I was told that they would be
shipping "around the end of October."

I have no connection with Bunton Instruments other than as a customer.

Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice






From daemon Fri Oct 6 10:18:53 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 06 Oct 2000 09:16:29 -0500
Subject: Re: Ge vs Si detectors - what are the shortcomings of Ge?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I thought the only drawback was cost. How much of a cost differential are
you being quoted?

I thought all manufacturers had to pick through crystals to find ones
suitable for EDS detectors in regard to dead layer, impurity, etc. If good
Ge chips are harder to fine, it would mean more expensive detectors.

We haven't tried thermally cycling our detector. I have heard that voltage
applied to Si(Li) detectors while warm will lead to a loss of Li, ruining
the detector. I don't know what effect it would have on a Ge detector.
However, my manager is reluctant to find out if there would be a problem so
we just keep our system cool.

Warren

At 09:10 AM 10/6/2000 +0100, you wrote:

} Thank you all for your replies on this topic. Several people seem to
} like Ge detectors for low keV work, and the replies have mostly been
} very positive about their resolution and performance. Some authors
} strongly advocate the use of Ge detectors for low kV, low probe
} current work with light element samples (See, for example, Block-
} van Hoek & Pinxter (1993) Chapter 1 in X-ray microanalysis in
} biology edited by Sigee et al, Cambridge University Press). Their
} opinion (and apparently yours) stands in contrast with the advice
} given by all of the XRMA vendors I am speaking to, whose opinion is
} that Si(Li) is the way to go (granted some of them don't sell Ge, so
} they would obviously have to advocate Si). So my question is "what
} is the Achilles heel of Ge detectors?". I am aware for example that
} their potentially very favourable characteristics at low kV are spoiled
} by the practical difficulty of manufacturing them without a large dead
} layer. Is this a fatal flaw in practice for analysis of elements with z
} {11? Are they less robust, less tolerant of thermal cycling? If
} anyone can help me get to the bottom of this I would be most
} grateful.
}
} Chris

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking


From daemon Fri Oct 6 10:34:42 2000



From: Christine Brantner :      brantner-at-codon.nih.gov
Date: Fri, 6 Oct 2000 11:42:23 -0400
Subject: CoolPix 950 to Axioscope 2 and cable release

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Good day

We have a new Nikon adaptor from Image Systems that allows us to
attach our CoolPix 950 to our Zeiss Axioscope 2 via the C-mount or
through the eye tube if we wanted to do it that way. Image Systems
also sells something they call a cable release adaptor which is a
"fancy piece" that hooks onto the camera over the shutter button so
that a cable release can be screwed in there. This way we can take a
picture without putting pressure on the adaptor set-up or programming
the camera to do it. Image Systems in Columbia Maryland can be
reached at 410-995-0748.

Just a recent customer.

Chris
--
:):):):):):):):):):):):):):):):):):):):):):):):):):):):):):)

Christine A. Brantner, Ph.D.

Biologist/Lab Manager

National Institutes of Health
National Institute of Neurological Diseases and Stroke
Lab of Neurobiology
Section of Analytical Cell Biology
Bldg 36, room 2A21 phone 301-435-2803
36 Convent Dr fax 301-480-1485
Bethesda, MD 20892-4062


From daemon Fri Oct 6 10:43:51 2000



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Fri, 6 Oct 2000 08:41:43 -0700 (PDT)
Subject: Re: Carbon Coated Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Nancy - Try Ladd Research (www.laddresearch.com) for high quality grids.
I've been using their C-coated grids for several years now and have found
that nearly every grid has been perfect. Their grids are somewhat more
expensive than those from other manufacturers, but well worth the price if
you need good quality.

I haven't tried purchasing grids from every manufacturer so there may be
other companies that also produce fine C-coated grids.

Dave Joswiak
Dept. of Astronomy
Univ. of Washington
Seattle, WA 98195
(206)543-7702
joswiak-at-astro.washington.edu


On Thu, 5 Oct 2000, Nancy_Robertson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi,
}
} I need good quality carbon coated grids for viewing purified plant virus
} particles with TEM. I have had little success with "store bought" coated
} grids. So unless someone could recommend a good cc grid source, I will
} need to buy a carbon coater. Should I purchase a carbon coater using
} flash evaporation or the more expensive carbon turbo evaporator?
} In addition, could anyone recommend an economically good microtome for
} thin sectioning plastic embedded specimens?
}
} Thank You,
}
} Nancy L. Robertson, Research Plant Pathologist
} USDA/ARS
} National Arctic Plant Germplasm Resources Unit
} 533 Fireweed Ave.
} Palmer, Alaska 99645
} phone: 907-746-9465
} fax:907-746-2677
} e-mail: nancy_robertson-at-dnr.state.ak.us
}
}



From daemon Fri Oct 6 10:50:35 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 06 Oct 2000 08:47:15 -0700
Subject: Re: SEM Help needed on conductive adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Carla,
The double-sided, conductive tabs that are sold in most EM supply catalogues
are excellent and provide a good, strong hold. I use DAG 154 from Acheson
Colloids, which is colloidal carbon in iso-propanol and ethanol, as a
conductive paint to connect the coated surface to the stub.
At 06:46 AM 10/5/00 -0700, you wrote:
}
} Hello all,
}
} I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with
} Electrodag 502 for secondary electron imaging. The adhesive is a graphite
} paint in MEK base (a substitute for TV Tube Koat). After ethanol
} dehydration and CP drying in liquid CO2, the samples are relatively flat or
} slightly curved. However, once attached to the stub the skins develop a
} noticeable curl which leaves a gap between the stub surface and sample. In
} some cases I can see the "trail" in the adhesive left by the skin as it was
} curling! Filling the gap with more Electrodag is a waste of time because
} it evaporates and shrinks back away from the sample (thankfully it doesn't
} seem to produce more curling).
}
} Could anyone suggest a better conductive adhesive? Also, is there any
} difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based
} media as to its effect on biological samples?
}
} As an SEM novice any advice you could share with me is greatly appreciated.
} Thank you very much,
}
} Carla Aiwohi
} Western Fisheries Research Center
} Seattle, WA
} ph: (206) 526-6282 ext.242
} fax: (206) 526-6654

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Oct 6 10:52:25 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Fri, 6 Oct 2000 08:39:04 -0700
Subject: RE: Digital microscope camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Robin writes ...

} } I'm looking to replace my two year old Polaroid Digital
} Microscope camera ...

If your camera-microscope interface is via 1x c-mount, then the Nikon
CP990 and the 1x c-mount adapter is a very good choice ... or convert
your microscope tube to c-mount (1x).

A point should be made ... the CP cameras acquire images to an
internal memory card ("Compact Flash") ... than cannot acquire direct
to a computer. The transfer is instead accomplished at some other
time ... either a direct connection, camera to a serial or USB port,
or (better/faster) by removing the memory card and using a "Compact
Flash" card reader. This might be a hassle for a workstation type
microscope, but if freedom of location is important, then probably
works better.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Fri Oct 6 11:54:30 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Fri, 6 Oct 2000 12:50:58 -0400
Subject: Re: Ge vs Si detectors - what are the shortcomings of Ge?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html






Dear Warren,

I thought the only drawback was cost.

Another drawback is the need to keep them at 77 K all the time (see below).

I have heard that voltage applied to Si(Li) detectors while warm will lead to a
loss of Li, ruining the detector.

Not actual loss of Li. To produce a Si(Li) detector, Li atoms are diffused
into a Si crystal, which is then subjected to a bias voltage and heated. Li
moves toward the cathode in such a way as to compensate for excess electron
acceptors, and this results in a large fraction of the detector volume being
depleted of carriers and, thus, having a very low dark current. When warmed to
room temperature in the presence of bias voltage, Li is redistributed, and the
detector no longer has the necessary large depletion region. It is possible to
get the same effect with ultra pure Si or Ge, but such detectors--called
"intrinsic"--are very expensive due to the stringent limit for impurities. It
is now known that in the absence of bias voltage Si(Li) detectors can be at room
temperature for a while without damage (although 15 years ago that was not
thought to be the case), but Ge(Li) detectors must be kept at 77 K even when no
voltage is applied.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Fri Oct 6 13:53:55 2000



From: Alan Fox :      fox-at-nps.navy.mil
Date: Fri, 06 Oct 2000 11:47:59 -0600
Subject: Ge vs SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Chris
I also have an Oxford intrinsic Ge detector on my SEM and enjoy
all the advantages described by previous correspondents. However, as the
max voltage on my SEM is 30kV the heaviest element for which I can get K
line info (which is preferable to L-lines for quantification) with
decent S/N ratio is around Sn (Z = 50). This of course depends on the
amount of tin present in your sample.

If you have a Ge detector fitted to a TEM operating at 200 kV, then, in
principle, you should be able to detect K lines for all elements. As a
result, installation of an intrinsic Ge detector on a TEM may also be a
good thing.

Alan Fox

Alan Fox
Center for Materials Science and Engineering
Naval Postgraduate School
Monterey
CA 93943

Tel (831) 656 2142
Fax (831) 656 2238



From daemon Fri Oct 6 14:04:59 2000



From: Dr. Raj Lartius :      rlartius-at-novascan.com
Date: Fri, 06 Oct 2000 14:03:41 -0500
Subject: Where to list a late model SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone,

My company is interested in selling a fairly new SEM and we are having a
difficult time finding an appropriate place to list it. Can anyone give
some suggestions. Also is there any kind of depreciation formula for
estimating an appropriate valuation.

Thanks

Raj

**********************************************
Dr. Raj Lartius, CEO
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Voice: 515-795-3164
Fax: 515-795-4414
**********************************************
"Innovative Tools to Explore the Microworld"


From daemon Fri Oct 6 14:20:09 2000



From: Norm Olson :      nho-at-bilbo.bio.purdue.edu
Date: Fri, 6 Oct 00 14:16:38 -0500
Subject: Freeze-plunge device availability

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


EM-listers: I frequently get asked for commercial sources for devices
that plunge freeze TEM grids into a cryogen such as liquid ethane. We
have our own in-house developed device so I have never kept up with what
is on the market. Available devices also seem to come and go so some of
my old sources no longer are current. Could I get vendors that have
these devices to respond to me off the list. I would appreciate a
reference to a web page if one exists and whatever ordering information
is available. I will be posting this information on our lab web page and
if there is enough interest on this list I can also post the responses.

Thanks
Norm Olson

*******************************************
Norm Olson
Senior Research Electron Microscopist
Department of Biological Sciences
Lilly Hall of Life Sciences
Purdue University
West Lafayette, IN 47907

Phone: 765-494-5643
FAX: 765-496-1189
email: nho-at-bragg.bio.purdue.edu
http://bilbo.bio.purdue.edu/~nho/index.htm
http://bilbo.bio.purdue.edu/~baker/

*******************************************



From daemon Fri Oct 6 14:37:28 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 6 Oct 2000 15:35:15 -0400
Subject: alternate fluid for ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
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Message-ID: {731740B45BFBD3119C3400D0B73C5E42F33016-at-sgofusr08.ccs.ppg.com}


What other liquids can be used for floating off thin sections other than
water? I have a sample that swells in water. It swells slowly in alcohol.
Thanks in advance.

-Scott Walck



From daemon Fri Oct 6 14:44:28 2000



From: Plybon, Charles :      Charles.Plybon-at-fwc.state.fl.us
Date: Fri, 6 Oct 2000 15:37:39 -0400
Subject: Lugol's Iodine as a Fixative

Contents Retrieved from Microscopy Listserver Archives
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I have been asked about the mechanism of Lugol's Iodine as a fixative and I
am not sure how it works. Is it a coagulent or anitcoagulent fixative?
Does it cross-bind proteins like formaldehyde or formalin? It is used often
for fixing dinoflagellate samples and it seems to stabilize the structure of
the organisms fairly well without alcohol. Can anyone explain to me the
mechanism?

Charles T. Plybon
Florida Marine Research Institute
Research Staff
Aquatic Health Group
Tel: (727) 896-8626
e-mail: charles.plybon-at-fwc.state.fl.us



From daemon Fri Oct 6 15:26:31 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Fri, 6 Oct 2000 16:15:54 -0400
Subject: L.R. WHITE

Contents Retrieved from Microscopy Listserver Archives
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List severs:

Does any one out there etch L. R. White sections? With
sodium-meta-periodate, or something else? Does it really make a difference?
Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Fri Oct 6 15:31:06 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 6 Oct 2000 13:22:56 -0700
Subject: Video microscopy needed

Contents Retrieved from Microscopy Listserver Archives
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Please reply directly to Somayyah:

Fabian-Baber Communications is a multimedia educational production
company. We are currently producing a ten-part video series on the human
body to be used in middle-school classrooms. We are looking for motion
microscopy of human cells, such as blood cells coursing through arteries,
cells during mitosis, etc. If you are interested in contributing to this
project, or know of a good resource for these images, please contact ,
Somayyah Siddiqi, at sam-at-fabian-baber.com or 610 623 7812. Thanks so much!

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Oct 6 16:27:32 2000



From: hadden-at-wingate.edu (Lee Hadden)
Date: Fri, 06 Oct 2000 17:26:13 -0400
Subject: CPD user feedback

Contents Retrieved from Microscopy Listserver Archives
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To CPD users:

I would appreciate the benefit of your experience and knowledge in
answering
several questions for me as we prepare to purchase our CPD for use in
teaching
undergraduate biology courses and student/faculty research.

What is your recommendation re the following:

having / not having a viewing port: [I've heard a few recommendations
for having a
viewing port in a CPD [all accompanied by warnings re the inherent
danger]. The
only CPD I've used [years ago] had a port which I liked, especially for
teaching
purposes. I also been asked "Why would you want one?"

chamber size and orientation: vertical or horizontal:

type[s] of specimen holders:

electrically heated vs. hot water heated:

manufacturers and models that you use or have used recently:

costs of fluids and materials used in / with your CPD for a typical run:

compared to other models you might be familiar with:
Specifically, is it too much more expensive to run a Polaron jumbo
chamber vs.
their regular size chamber? [again, for biological material].

What would you look for in your next CPD?

Thanks for your input and time -- they're greatly appreciated.

Lee Hadden
Department of Biology
Wingate University
Wingate, NC 28174
hadden-at-wingate.edu
704.233.8238.





From daemon Fri Oct 6 16:30:09 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Fri, 6 Oct 2000 17:24:05 -0400 (EDT)
Subject: Re: L.R. WHITE

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If you are trying to "antigen retrieve" on LR White, there is a nifty
paper - Stirling and Graff, 1995, Journal of Histochem. and Cytochem.,
43:115-123. "Antigen Unmasking for Immunoelectron Microscopy: Labeling is
Improved by Treating with Sodium Ethoxide or Sodium Metaperiodate, then
Heating on Retrieval Medium"

Tamara Howard
CSHL

On Fri, 6 Oct 2000, Timothy Schneider wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} List severs:
}
} Does any one out there etch L. R. White sections? With
} sodium-meta-periodate, or something else? Does it really make a difference?
} Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu
}
}
}



From daemon Fri Oct 6 20:12:14 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 06 Oct 2000 21:12:28 -0500
Subject: carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Nancy Robertson wrote:
===============================================================
} I need good quality carbon coated grids for viewing purified plant virus
} particles with TEM. I have had little success with "store bought" coated
grids.
} So unless someone could recommend a good cc grid source, I will need to
buy a
} carbon coater. Should I purchase a carbon coater using flash evaporation
or the
} more expensive carbon turbo evaporator? In addition, could anyone
recommend an
} economically good microtome for thin sectioning plastic embedded specimens
?
================================================================
SPI Supplies has produced, in house, with our own (in-house) experienced
staff, carbon coated grids, including holey and lacey types, for many years.
I hope your less-than-expected results from purchased grids were not our
grids, because we really are set up to make them however one does want to
make them, mainly because we have our own TEM facilities at our side to
inspect what we have made. In any case, many laboratories worldwide depend
on the SPI carbon coated grids and we are not aware of any problems that
anyone has encountered with them (lots not passing inspection are rejected
and not sent to customers). Some want them thicker, some want them thinner,
some want them with large holes, some smaller holes, but we can make them
however someone wants them to be!

With regard to your question: "Should I purchase a carbon coater using
flash evaporation", I will assume that you are asking about the "carbon
coaters" that are operated with a rotary vane mechanical pump, usually in
tandem with a sputter coater. We are not aware of anyone that has ever
gotten acceptable carbon coatings that could be used as support films by TEM
using such an equipment approach. Only a diffusion pumped vacuum system, or
better, seems to be capable of producing the kind of carbon coatings that
would meet that standard. A number of firms offer vacuum evaporators, and
we have found the vacuum evaporators made by VG Microtech and also, Denton
Vacuum to be especially good for this kind of applications. Quite possibly,
others are equally good, but our experience has been with these two
particular manufacturers. Note: We do not have any interest, financial or
otherwise, in either Denton Vacuum or VG Microtech.

We had the good fortune of learning about a paper soon to be published
comparing carbon grids from different commercial sources. The author is P.
J. F. Harris, Dept. of Chemistry, U. Reading, Whiteknights, Reading RG6 6AD
UK. The title is: Carbonaceous Contaminants on Carbon Support Films for
Transmission Electron Microscopy, Carbon 1 (2000).

If Dr. Harris is correct, then one possible solution, since they are made
using a different approach (but were not studied by Dr. Harris), might be
the Quantifoil grids (my opinion), see URL
http://www.2spi.com/catalog/grids/quantifoil-carbon-films.html

They are produced using a different kind of process and therefore would be
expected to not have present some of the ambiguous features found on the
carbon films studied in the paper mentioned above.

I don't know if this paper has been published yet or not, but I could send
to any one requesting it, the entire preprint of the paper via pdf file.
Such requests should be made off-line. Give me a day or two to respond.

Disclaimer: SPI Supplies has manufactured since 1975 custom made carbon
support films for TEM applications. Grids made by SPI were one of those
studied in the above referenced article. However, it is entirely possible
that given some of the new demands being made on carbon coated grids
generally, especially for high temperature stability, that one should
consider as an alternative, the SPI Silicon Nitride Membrane Grids, as
described on URL
http://www.2spi.com/catalog/instruments/silicon-nitride.html

Chuck

===========================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Fri Oct 6 20:12:14 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Sat, 7 Oct 2000 11:06:01 +1000
Subject: RE: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
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Allen R. Sampson wrote:
}
} Have an EDS system attached? The EDS dewars, being generally supported by
} a cantilever structure, can be quite effective at coupling acoustic
} vibrations into an SEM. The large area and thin metal construction of the
}

EDS dewars:
In regard to EDS dewars and acoustic vibrations, if there is enough ice or
dirt in the dewar to nucleate bubbles in the LN2 then you might get
sufficient vibration as the LN2 boils off. You can often hear this simply
by putting your ear against the dewar, especially while the LN2 is at a
relatively low level in the dewar. Perhaps in really vibration sensitive
systems such as a FESEM this could be another potential problem? The sound
of the boiling can range from a continuos fizz to an occasional intense
"bump" similar to the effect that you can get just as water begins to boil
in a pot on a stove.

Stage motors:
We had a bad problem with stage motors once. If you have a motorised stage
then any AC ripple from bad power supply filtering remaining on top of the
DC holding current can transmit enough vibration via the motor coils to
cause problems at high magnification. Sometimes you can "feel" these kinds
of vibrations in the motor bodies. Since this holding current is present
even when the motors are not actually moving the stage, you have to
actually shut off the power to the stage motors to determine if this is the
cause.


Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Sat Oct 7 07:26:55 2000



From: MESnesm-at-aol.com
Date: Sat, 7 Oct 2000 08:16:55 EDT
Subject: NESM October Meeting

Contents Retrieved from Microscopy Listserver Archives
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To all:

The October meeting of NESM (New England Society for Microscopy) will be held
on October 17th at FEI Company in Peabody, MA.

Pre-registration deadline is Friday, October 13th. Please contact Mary
McCann, NESM Treasurer at (617)484-7865 or by e-mail: mccanns-at-tiac.net.
Registration is $5.00 for members and $20.00 for non-members (this fee
includes a current-year membership in NESM).

The meeting will begin with a tour of FEI Company at 5:30pm. Also at this
time, Dr. Ronald Mueller of Bristol Myers Squibb will speak on the utility
of SEM within
the Pharmaceutical Industry. A buffet dinner will follow at 6:00pm.

The scientific presentations will begin at 7:00pm. The speakers are:
Dr. Ben Fabry of Harvard School of Public Health, Boston, MA-"Magnetic
Twisting
Cytometry with Sub-Micron Optical Detection"

Dr. Dan Friend of Brigham and Women's Hospital, Boston, MA-"Thin Section:
Freeze Fracture Correlates-A Visit to the Good Old Days"

Barbara Foster-President, Microscopy, Marketing and Education, Springfield,
MA-
"Bridging the Microscopy-Spectroscopy Chasm"

Peggy Sherwood
Corresponding Secretary, NESM



From daemon Sat Oct 7 11:28:15 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 07 Oct 2000 09:19:35 -0700
Subject: Options for Coolpix 990 to Zeiss (long)

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who sent suggestions and options for
connecting the CP990 to Zeiss Axioskop. There are
several options. After examining each one, here is
a good approach to using the CP990 on an Axioskop.

The first accessory is the attachment of the camera
to the 'scope. This is done using an Axioskop trinoc
photo port adapter to C-mount. Then, a CP990 thread
ring to C-mount adapter is installed. These two
adapters then mate the CP990 to the photo port of
the Axioskop. Optmem makes all of the pieces needed
to make the mating between 'scope and camera.
The mate from photo port to C-mount can be done two
ways.

Diagnostic Instruments makes a 38mm ISO mount
(PN DZA, $275) which also accepts items for Leitz.
This same ISO mount onto the Zeiss will accept
a PA-41A adapter for Nikon F mount cameras.
Optem makes a coupler DC10NN ($80) which
mates the 38mm ISO port to C-mount. Then,
their 25-70-14-03 ($320) is the CP990 to C-mount
adapter. This completes the adaption for a total
cost of $275 + $80 + $320 = $675.

Optem's alternate method is the DC-10AM ($150)
which directly mounts in the trinoc photo port and
provides a C-mount. Then, this mates with the
same 25-70-14-03 CP990/C-mount adapter.
Total cost of this approach is $150 + $320 = $470.

Zeiss also makes an Axioskop photo port C-mount
adapter. This part number is 45 29 95. They
can be found used from between $125-$250.
Then, the Optem 25-70-14-13 CP990 adapter is
used to complete the mate.

When using the CP990, or any other camera for that
matter, vibration is a key issue. In this respect the
CP990 has an advantage since it does not have a
mechanical shutter as do other CCD cameras.
But one still needs to reduce vibration when actually
taking a pix. This is done via a remote cable release.
There are several aftermarket units which perform
this function. However, they are rather awkward and
large. The best approach is the Nikon EU-1 wired
remote ($195 list price). This is a small hand held
puck which plugs into the USB port on the CP990.
Pressing the large button down half way is like
doing so on the CP990 shutter release button. Pressing
all the way down takes the pix. The puck also indicates
on an LCD how many frames are left.

When doing 'scope work, I recommend the Nikon EH-31
AC adaptor ($50 list price). This avoids drain on the
camera batteries.

For storage, the CP990 uses CF type 1 media. The IBM
microdrive will not work in this camera. Since the
camera stores images in JPEG format, the file size of
each image will vary according to how the image
compresses. So far, the largest file I have taken is
just over 1MB. I am using 128MB CF media. So I can
reasonably expect to get about 100 frames per CF.
I have two of these CF media. When one is full, it
is pulled and replaced with an empty one. The full one
is emptied using a Microtech USB CF reader ($38).

Finally, image focusing has always been a problem for me.
The CP990 changes all of that. Using the CP990 real time
NTSC video output jack, one can watch the image on a
separate TV monitor (b/w or color) and adjust for perfect
focus. Then take the pix. If you want the camera's LCD
unit to stay on indefinitely while shooting, use the setup
menu and disable the power off feature.

That's about it for the CP990. Next is the Fuji Finepix S1 Pro.
One camp says it will work on a microscope. Another
camp says it won't. The camera only does automatic
modes when using a lens. When the lens is removed,
the camera becomes dumb. What is will actually do
remains to be seen. The folks at ElectroImage say
that it does work.

Contacts: Ron; Optmem NY. 716.223.2370
Mike Reyes, Diagnostic Instruments. Extension 32
Zeiss....good luck.

cheers,
gary g.





From daemon Sat Oct 7 14:35:57 2000



From: Plybon, Charles :      Charles.Plybon-at-fwc.state.fl.us
Date: Sat, 7 Oct 2000 15:26:49 -0400
Subject: Lugol's Iodine as a Fixative

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For some time, Lugol's Iodine has been used not only to stain certain cells
but also to fix and preserve samples of dinoflagellates and other
zooplankton. I am curious as to what the mechanism is for stabilizing and
fixing with Lugol's. Coagulent or anitcoagulent, does it cross-bind
proteins preserving cellular integrity? Can anyone help?

Charles T. Plybon
Florida Marine Research Institute
Research Staff
Aquatic Health Group
Tel: (727) 896-8626
e-mail: charles.plybon-at-fwc.state.fl.us



From daemon Mon Oct 9 01:00:33 2000



From: klaus neumann :      bikneu-at-krzsun.med-rz.uni-saarland.de
Date: Mon, 09 Oct 2000 08:00:03 +0200
Subject: alternate fluid for ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
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To Scott Walck:
Why not try a DRY (diamond) knife?
Klaus Neumann
Med. Biologie
Universität des Saarlandes
D-66421 Homburg
Germany




Dr. Klaus Neumann
3.5 Med. Biologie
Universität
D-66421 Homburg
Tel: ++49-6841-16-62 55
Fax: ++49-6841-16-62 56
http://www.med-rz.uni-sb.de/med_fak/biologie



From daemon Mon Oct 9 08:31:48 2000



From: Marek Malecki :      mmm-at-biomail.ucsd.edu
Date: Mon, 9 Oct 2000 08:27:56 -0500
Subject: Re: alternate fluid for ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
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Scott,
Glycerol at different concentration (87% as a starter). I use it for PVP
and PVA blocks.
Marek.


At 15:35 2000-10-06 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Marek Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California, San Diego

address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA92093-0368
phone - office: 8588223373
phone - lab: 8588223715
fax: 8588223715
e.mail: mmm-at-ucsd.edu
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil.ucsd.edu




From daemon Mon Oct 9 09:38:40 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Mon, 9 Oct 2000 07:16:21 -0700
Subject: RE: Options for Coolpix 990 to Zeiss (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Gary writes ...

} For storage, the CP990 uses CF type 1 media. The IBM
} microdrive will not work in this camera. Since the
} camera stores images in JPEG format, the file size of
} each image will vary according to how the image
} compresses. So far, the largest file I have taken is
} just over 1MB.

First ... thanx for compiling this info. However and regarding the
statement above ... are you sure the CP990 does not provide for saving
TIFFs? My CP800 will save a single high res TIFF to a 8Mb memory
card. Presumably one could save ~8-10 CP990 TIFFs to a 128Mb CF
card(?) Get back to us on this one ... I'm afraid "JPEG only" would
dissuade those who believe in "non-lossy" TIFFs only.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Mon Oct 9 10:12:37 2000



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Mon, 09 Oct 2000 10:10:45 -0500
Subject: SeaLite info

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
Sorry to bother you with such a strange request. Does anyone have contact
information for SeaLite Sciences (associated with bioluminescent markers)?
All I have is a web site, and after several days of trying to get through,
I've just about given up.
Thanks for your help once again,
Kristen



From daemon Mon Oct 9 11:10:44 2000



From: John andrew :      johnpsdu-at-yahoo.com
Date: Mon, 9 Oct 2000 09:08:46 -0700 (PDT)
Subject: Sputter Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
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I need to purchase a sputter coater for under 5K. Can
this group recomend what is a good purchase.

Thank you,
John

__________________________________________________
Do You Yahoo!?
Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free!
http://photos.yahoo.com/


From daemon Mon Oct 9 11:15:16 2000



From: John andrew :      johnpsdu-at-yahoo.com
Date: Mon, 9 Oct 2000 09:12:30 -0700 (PDT)
Subject: Sputter Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I need to purchase a sputter coater for under 5K. Can
this group recomend what would be a good purchase.

Thank you

__________________________________________________
Do You Yahoo!?
Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free!
http://photos.yahoo.com/


From daemon Mon Oct 9 11:15:33 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 09 Oct 2000 08:09:40 -0700
Subject: RE: Options for Coolpix 990 to Zeiss (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


At 07:16 AM 10/9/00, you wrote:
} Gary writes ...
}
} } For storage, the CP990 uses CF type 1 media. The IBM
} } microdrive will not work in this camera. Since the
} } camera stores images in JPEG format, the file size of
} } each image will vary according to how the image
} } compresses. So far, the largest file I have taken is
} } just over 1MB.
}
} First ... thanx for compiling this info. However and regarding the
} statement above ... are you sure the CP990 does not provide for saving
} TIFFs? My CP800 will save a single high res TIFF to a 8Mb memory
} card. Presumably one could save ~8-10 CP990 TIFFs to a 128Mb CF
} card(?) Get back to us on this one ... I'm afraid "JPEG only" would
} dissuade those who believe in "non-lossy" TIFFs only.

Yes, it does have a TIFF mode. But this is only available via the
Manual mode. I figured that most folks wanted quick and easy
access to image capture. If they want/need TIFF, they will
have to attack the manual mode.

gary



From daemon Mon Oct 9 11:30:16 2000



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Mon, 09 Oct 2000 09:29:57 -0700
Subject: Re: TEM CCD camera

Contents Retrieved from Microscopy Listserver Archives
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Larry:
We would be very interested in hearing of what you come up with once you have a
solution.
Mike O'Keefe

Larry Allard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Alan:
}
} Just this morning we were discussing the same problem with our Gatan
} CCD cameras on our HD-2000, one of which is retractable and used for
} TEM imaging, and the other of which is at the end of the GIF for
} energy-filtered imaging. These cameras are connected in series for
} the water flow, and have very tiny water lines which can easily clog,
} even with recirculated water. There is no provision by the
} manufacturer to provide for a safety cut-off of the Peltier cooler in
} the event of loss of water flow. We have recently experienced a
} burn-out of the TEM camera due to a clog in the line, which resulted
} in 2 weeks of downtime for repair (nicely done for free by Gatan),
} but still no response to queries about some sort of protection. So
} we have decided to find an appropriate water flow sensor, and to
} install it in an appropriate place in the water line, and to connect
} it to the camera controllers to provide a power cut-off when the
} water flow diminishes. I'll post the details of the solution when it
} is completed.
}
} Larry
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listers
} }
} } Have any other microscopists had problems with Peltier coolers failing on
} } water cooled CCD cameras?
} }
} } In my facility I have three such cameras all plumbed into the microscope
} } water system and connected, by the camera manufacturer, into wall sockets.
} } On one of the three cameras I have had the Peltier cooler fail four times
} } in two years. After the latest failure the manufacturer asked if the
} } camera had been run without water. I told him not intentionally but when
} } we have power cuts and the power is restored, the camera comes back on but
} } the microscope (and water) does not. The camera can therefore run for some
} } time without water cooling. Power failures are not uncommon at this
} } university!
} }
} } Many e-mails to the manufacturer have resulted in no response to my
} } questions about reliability of the Peltier coolers. The manufacturer is
} } treating the failure as an out of warranty issue but I believe there has
} } been something wrong with this camera since it was installed.
} }
} } Any experiences of listservers with Peltier cooler failures would be
} } appreciated. Please e-mail me directly.
} }
} } Regards
} }
} } Alan W Nicholls, PhD
} } Electron Microscopy Service Director
} } Research Resources Center - East (M/C 337)
} } Room 100 Science and Engineering South Building
} } The University of Illinois at Chicago
} } 845 West Taylor St
} } Chicago, IL 60607-7058
} }
} } Tel: 312 996 1227
} } Fax: 312 996 8091
} } Office: Room 110
} }
} } Web site www.rrc.uic.edu
} }
} }
} } Alan W Nicholls, PhD
} } Electron Microscopy Service Director
} } Research Resources Center - East (M/C 337)
} } Room 100 Science and Engineering South Building
} } The University of Illinois at Chicago
} } 845 West Taylor St
} } Chicago, IL 60607-7058
} }
} } Tel: 312 996 1227
} } Fax: 312 996 8091
} } Office: Room 110
} }
} } Web site www.rrc.uic.edu
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413 Fax
} allardlfjr-at-ornl.gov



From daemon Mon Oct 9 11:38:46 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Mon, 09 Oct 2000 11:50:19 -0500
Subject: Texas Society for Microscopy 2000 Fall Meeting

Contents Retrieved from Microscopy Listserver Archives
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The 2000 Fall Meeting of the Texas Society for Microscopy will be held
on October 26, 27, and 28
at the DoubleTree Hotel in Dallas, TX. The hotel is located in
Campbell Centre at 8350 N. Central
Expressway, Dallas, TX 75206. The phone number for the hotel is
800-222-TREE.
A special workshop will be held on Thursday, Oct. 26, entitled "The
SEM Today". It will cover
the latest information concerning basic SEM, LVSEM, ESEM and
detectors.

For more information and/or to register, please contact
Pamela Neill, Program Chair TSM
Alcon Research LTD
6201 South Freeway
Forth Worth, TX 76134-2099
pamela.neill-at-alconlabs.com


--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Mon Oct 9 13:05:46 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Mon, 09 Oct 2000 14:03:01 -0400
Subject: Re: Sputter Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,

We at Ladd may be able to help you. Please let us know what type of work
you want to do with it ((target, size of sample, type of sample prep,
etc) and I'll let you know what we have available.

Best Regards,

John Arnott


LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Microscope Supplies Since 1955



John andrew wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} I need to purchase a sputter coater for under 5K. Can
} this group recomend what would be a good purchase.
}
} Thank you
}
} __________________________________________________
} Do You Yahoo!?
} Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free!
} http://photos.yahoo.com/

--


From daemon Mon Oct 9 13:16:10 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Mon, 09 Oct 2000 14:14:06 -0400
Subject: Re: Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


We appreciate the comments Dr. Joswaik and others concerning the quality
of Ladd coated grids.
Since there are still a number of researchers who coat their own grids,
we are pleased to share some of the things we have learned over the past
45 years.

1. Clean the evaporator at least once a day.
2. Change the diffusion pump oil at least every two weeks.
3. Inspect and clean each individual grid prior to coating.
4. Out gas the garbon prior to evaporation.
5. Use fresh chemicals with each batch of grids to be coated.

Hopefully some of this will be of assistance.
A recent posting mentioned that a diffusion pump system should be used
for carbon coating. We agree 100% with that. In fact we designed the
Ladd vacuum system with coated grids in mind by providing for "quick
changing" the oil and speedy clean-up.

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Microscope Supplies Since 1955


From daemon Mon Oct 9 13:18:56 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Mon, 09 Oct 2000 14:13:12 -0400
Subject: TEM for horse tendons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
We received several VERY large pieces of horse tendon, half of which
was immersed in ethanol and the rest in formalin. The vet didn't know
which would be best , so that's how it arrived, via air from New
Mexico. We cut it into 1 x 3 mm pieces and processed it routinely, ie,
glut, OsO4, ethanol dehydration and PO. We infiltrated in Spurr's and PO
for 24 hrs, straight Spurr's for 2 hours and then embedded. What was left
just fell out of the blocks . Needless to say, we have a lot of tissue left
(still in EtOH and fomalin) and I was wondering if it's worth trying to
salvage and if anyone has any suggestions as to the best way to handle
this. I did ask the client to please contact me before sending any more
tissue but for now they would like us to try and get something from this.
Any ideas would really be appreciated.
Thank you,

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky


From daemon Mon Oct 9 13:43:39 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Mon, 09 Oct 2000 11:40:49 -0700
Subject: Bacterial quantitation in soil samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




We are considering purchase of a system for quantitative analysis of bacterial
and fungal biovolume in soil samples. Could anyone provide info on equipment
and preferred method (FITC labeling?). As always, I appreciate your help,
Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321



From daemon Tue Oct 10 07:26:39 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Tue, 10 Oct 2000 08:14:58 -0400
Subject: more info: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


hi again-

before i go through the effort of separating the ground wire from the rest
of the supply system just a few more tidbits to throw out to the list:

1- the distortion in the scan seems to be about 60Hz (more cycles at
slower scan rates). in reduced area raster (about 4 frames/sec??) the
count is about 15. in "TV" (whatever rate that is on a 35) i get about 2
or 3 cycles.

2- the amplitude of the distortion increases a bit with decreasing KV
(i.e. its worse at 10KV than at 30KV...but not much worse). and it seems
to be worse at higher magnifications (at 10KX it just about covers the
screen left to right; at 500X it is just a couple of bumps).

3- i tightened all the ground lugs....no change.

4- i tightened all the console connectors...no change.

5- i moved the variac farther away from the console...no change.

6- perhaps unrelated.....the conformation of the "spot" realized when the
condenser lens current is decreased looks funny. i'm used to a realtively
uniform circular cross section. this one looks like a central uniform area
with dendritic "arms" radiating outwards...kinda like a spider web pattern.
does this mean anything to anyone?? (the final apertures are pretty
clean, and it looks funny with any of the three apertures)

thanks for all your previous comments...i'm sure to be closing in on fixing
this with your help.

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875


From daemon Tue Oct 10 08:55:38 2000



From: Malis, Tom :      malis-at-NRCan.gc.ca
Date: Tue, 10 Oct 2000 09:51:32 -0400
Subject: RE: alternate fluid for ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Scott. 'Bonne chance', as we say in Canada. A true water substitute (ie
in both knife edge wetability and surface tension) is one of those Holy
Grail things in ultramicrotomy. You could experiment with liquids like
alcohol mixtures, ethylene glycol, etc, but my suggestion is to save
yourself a lot of time and simply dry section. It's done more often than
you think; water-soluble minerals, reactive metals like Mg, to name a few.
Place a grid on the lower part of the diamond knife, section, then tease the
section onto the grid with your hair tool. An important point is to then
wet the section down onto the grid with something inert (perhaps an
alcohol), then dry under a lamp, so that it doesn't fall off the grid on
insertion into the TEM. If there is a danger of a somewhat brittle material
breaking apart upon sectioning, use a coated grid and some of the segments
should be retained.

Do let the Listserver know if someone out there suggests something that
works as well as water. You'll have a lot of friends at the next MSA
meeting!

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca



----------
From: Walck, Scott D. [SMTP:walck-at-ppg.com]
Sent: Friday, October 06, 2000 3:35 PM
To: 'Microscopy-at-MSA.Microscopy.Com'
Subject: alternate fluid for ultramicrotomy


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.


What other liquids can be used for floating off thin sections other
than
water? I have a sample that swells in water. It swells slowly in
alcohol.
Thanks in advance.

-Scott Walck



From daemon Tue Oct 10 10:43:38 2000



From: sghoshro-at-nmsu.edu
Date: Tue, 10 Oct 2000 09:39:16 -0600 (MDT)
Subject: bacteria protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Just wanted to say thank you for the many replies to my TEM/SEM problem
with bacterial culture protocol. Problem solved, thanks again.
Kristine C. Fambrough
NMSU



From daemon Tue Oct 10 11:23:23 2000



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Tue, 10 Oct 2000 09:25:01 +0100
Subject: Re: alternate fluid for ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Many years ago, I was trying lipid retention embedding for TEM of
mycobacterium. GACH seemed to work but sectioning into water was a
nightmare. It attracted water from the trough during the
ultramicrotome's return stroke which then caused the block to swell.
Freon worked better, but the sample from DuPont was quite volatile and
evaporated quickly. The workaround was to section into water, but to
coat the sides of the block with the thinnest possible film of silicone
grease. The grease provided sufficient hydrophobicity to prevent water
from jumping onto the face, and if I worked quickly the sections could
be placed onto the grids. Needless to say, I only used a glass knife
for this. It was a short project, so column contamination from
volatiles in the grease wasn't a problem. Maybe a sample of the grease
could be put under a high vacuum beforehand to remove volatile
components.

Regards,
Glen

"Malis, Tom" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Scott. 'Bonne chance', as we say in Canada. A true water substitute (ie
} in both knife edge wetability and surface tension) is one of those Holy
} Grail things in ultramicrotomy. You could experiment with liquids like
} alcohol mixtures, ethylene glycol, etc, but my suggestion is to save
} yourself a lot of time and simply dry section. It's done more often than
} you think; water-soluble minerals, reactive metals like Mg, to name a few.
} Place a grid on the lower part of the diamond knife, section, then tease the
} section onto the grid with your hair tool. An important point is to then
} wet the section down onto the grid with something inert (perhaps an
} alcohol), then dry under a lamp, so that it doesn't fall off the grid on
} insertion into the TEM. If there is a danger of a somewhat brittle material
} breaking apart upon sectioning, use a coated grid and some of the segments
} should be retained.
}
} Do let the Listserver know if someone out there suggests something that
} works as well as water. You'll have a lot of friends at the next MSA
} meeting!
}
} Tom Malis
} Group Leader - Characterization
} Materials Technology Laboratory
} Natural Resources Canada (Govt. of Canada)
} 568 Booth St., Ottawa, Canada
} ph. 613-992-2310
} FAX 613-992-8735
} email: malis-at-nrcan.gc.ca
}
} ----------
} From: Walck, Scott D. [SMTP:walck-at-ppg.com]
} Sent: Friday, October 06, 2000 3:35 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: alternate fluid for ultramicrotomy
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
}
} What other liquids can be used for floating off thin sections other
} than
} water? I have a sample that swells in water. It swells slowly in
} alcohol.
} Thanks in advance.
}
} -Scott Walck
}

--

Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156
(206) 616-1828 fax
************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
************************************************************************


From daemon Tue Oct 10 12:09:49 2000



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Tue, 10 Oct 2000 18:09:12 +0100 (WAT)
Subject: SEM: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello Guys
I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
just installed in our central science lab.
Is there a way to clean stubs for reuse or are stubs disposable once used.
( This may sound naive)
soji
Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.
email: sojilori-at-oauife.edu.ng




From daemon Tue Oct 10 12:51:57 2000



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Tue, 10 Oct 2000 13:48:56 -0400
Subject: JSM 840 users and Analysis contract labs in the Atlanta area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I'm tring to find users of JEOL JSM-840 (or similar) microscopes in
the Atlanta area. In addition, I need to find contract SEM analysis labs
here that might be able to run some fast turnaround samples for me (mostly
cross sections). I know several of you contacted me recently after I posted
for a microscopist, but I no longer have the information. Could you please
send mail again to sbuckingham-at-excellatron.com .
Many Thanks,
Steve

Steve Buckingham
Excellatron Solid State LLC
1460 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 617 812 5920



From daemon Tue Oct 10 12:59:42 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 10 Oct 2000 10:56:21 -0700
Subject: Re: more info: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Brian,
The profile of the spot you describe sounds like an under-saturated filament.
At 08:14 AM 10/10/00 -0400, you wrote:

} hi again-
}
} before i go through the effort of separating the ground wire from the rest
} of the supply system just a few more tidbits to throw out to the list:
}
} 1- the distortion in the scan seems to be about 60Hz (more cycles at
} slower scan rates). in reduced area raster (about 4 frames/sec??) the
} count is about 15. in "TV" (whatever rate that is on a 35) i get about 2
} or 3 cycles.
}
} 2- the amplitude of the distortion increases a bit with decreasing KV
} (i.e. its worse at 10KV than at 30KV...but not much worse). and it seems
} to be worse at higher magnifications (at 10KX it just about covers the
} screen left to right; at 500X it is just a couple of bumps).
}
} 3- i tightened all the ground lugs....no change.
}
} 4- i tightened all the console connectors...no change.
}
} 5- i moved the variac farther away from the console...no change.
}
} 6- perhaps unrelated.....the conformation of the "spot" realized when the
} condenser lens current is decreased looks funny. i'm used to a realtively
} uniform circular cross section. this one looks like a central uniform area
} with dendritic "arms" radiating outwards...kinda like a spider web pattern.
} does this mean anything to anyone?? (the final apertures are pretty
} clean, and it looks funny with any of the three apertures)
}
} thanks for all your previous comments...i'm sure to be closing in on fixing
} this with your help.
}
} brian
} ----------------------------------------------
} Brian McIntyre

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Oct 10 15:23:33 2000



From: Tim Lyden :      lyden.11-at-osu.edu
Date: Tue, 10 Oct 2000 16:31:28 +0100
Subject: What's up...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Where is everyone.... I have not gotten a posting since yesterday????



From daemon Tue Oct 10 17:11:15 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Tue, 10 Oct 2000 15:09:14 -0700
Subject: RE: more info: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Brian writes ...

} ...
}
} 1- the distortion in the scan seems to be about 60Hz

This might imply you have a ground loop problem. Possibly one of the
other devices (computer, EDX, ...) is providing a ground connection
independent of your primary ground(?)

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Tue Oct 10 17:42:44 2000



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Tue, 10 Oct 2000 18:40:31 -0400 (EDT)
Subject: digital imaging info needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


)( / _ Jonathan Lipkin
() \ _ Assistant Professor of Multimedia Design
)( / _ Ramapo College of New Jersey
() \ _ http://arts.ramapo.edu/lipkin
)( / _ 201/ 684/ 7056/

Dear listserv:

Dee Breger was kind enough to forward this message for me. I'm writing a book
on digital photography, to be published by Harry N. Abrams next year. The book
will include a section on applied digital imaging, and I am particularly
interested in scientific imaging. I am hoping that some of you folks might
be able
to provide some information.

For the purposes of the book, I am defining digital imaging as that which
describes a scene using discrete digital information in a raster grid, or
bitmap. So, I am particularly interested in scientific imaging applications
which gather information in this way.

I would be interested to hear if there are other scientific imaging systems
(DEE: besides SEMs) which collect information in this manner. For instance,
it sounds as though atomic force microscopes work in this way: as they
drag a stylus across a surface, the position is recorded (x and y), along
with the height (z). This
is later used to re-create the image in a raster grid (the fact that the AFM
does not use light makes it another interesting foil to 'traditional' digital
photography). How then, are colors in the final output determined from the
data collected? I would also, of course, love to see any images you might have
to show.

Thanks in advance,
Jonathan Lipkin (jlipkin-at-ramapo.edu)

DEE: I'll forward to Jonathan any replies that are publically posted to the
listserver. He also needs other detailed technical information on digital
image formation which can be far more comprehensively clarified and
amplified by some of the experts among my fellow listers than by me, though
I've already given him the broad picture for analog scopes like my dear old
Cambridge 250. His SEM impressions, which I'll pass along verbatim,
include (1) "SEMs collect information, used to fill a raster grid, through
the use of a PMT", (2) the signal is sent directly to the crt in analog
form -I always had thought that the pmt sent out digital info, but it
sounds like it just sends out analog data (a waveform? though it couldn't
be a waveform if the raster grid is discrete in two dimensions)" and (3)
"as the secondary electrons come off the sample, they hit a detector which
generates a digital number (redundant) which is then placed in a pixel of a
bitmap."

Many thanks to any who can help!





***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Tue Oct 10 17:55:38 2000



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Tue, 10 Oct 2000 18:09:54 -0500 (CDT)
Subject: Ultrasonic Disc Cutter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone in the Chicago area have an ultrasonic
disc cutter we can use temporarily? Alternatively,
does anyone have one they are not using and would
be willing to sell or donate?

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++



From daemon Tue Oct 10 18:09:20 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Tue, 10 Oct 2000 19:07:23 -0400
Subject: Ultrasonic Disc Cutter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Marks:

If you aren't able to find an ultrasonic cutter locally to use, please let
me know. Perhaps I can arrange to have one of our SoniCut 380 systems sent
from our applications lab for short term use or maybe we could arrange to
cut some samples for you here. Please let me know if I can be of help.

Best regards-

David
Writing at 3:46:01 PM on 10/10/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "L. D. Marks"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anyone in the Chicago area have an ultrasonic
disc cutter we can use temporarily? Alternatively,
does anyone have one they are not using and would
be willing to sell or donate?

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++

{


From daemon Tue Oct 10 18:53:32 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Tue, 10 Oct 2000 16:49:43 -0700
Subject: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Well we've got a new vacuum evaporator and it has been so long since I've
had one I've forgotten how to treat the bell jar to reduce the effort it
takes to keep it clean. However, I do recall that there were little tricks
to cleaning and treating the glass and other surfaces. Anyone care to shed
a little light my darkened memory of these procedures?

TIA

Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Tue Oct 10 19:14:59 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 10 Oct 2000 19:05:05 -0500
Subject: Administrivia: Subscriber List Restored

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


G'day All...

Another fault this weekend, this time the subscription list
became corrupted. This mean't a significant number of you
(~ 1500 ) "disappeared" for a few days and hence did not receive mail.
Iunfortunatley did not notice since the mail did go through to the rest
of the subscribers on the list. In any event , I have restored the list to
it's Oct 6th backup copy.

To those of you that unsubscribed between Oct 6th and
Oct 10th. I apologize and ask you to unsubscribe again.
I, unfortunately, do not keep records of those of you that
have left.

Sorry...

Nestor
Your Friendly Neighborhood SysOp




From daemon Tue Oct 10 21:19:14 2000



From: NGUYEN THI VAN ANH :      vasnf-at-biochem.tohoku.ac.jp
Date: Wed, 11 Oct 2000 11:15:24 +0900
Subject: Cell adhesion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I am trying to get the internal(cytoplasmic) side of a prepared erythrocyte
ghost's plasma membrane attached to coverslip, so that the external side is free
in water solution. Does anyone have an experience with this or suggest the
reagent that preferably interacts with inner leaflet of plasma membrane or
cytoskeletal protein ? How should I test the external side is free ?

Any suggestions on this problem would be greatly welcomed. Thank you

Nguyen

Lab. of Appl. Microbiology
Dep. of Biochemistry
Faculty of Agriculture
Tohoku University
Sendai, Japan.






From daemon Tue Oct 10 22:02:17 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Wed, 11 Oct 2000 12:55:04 +1000
Subject: Re: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The easiest way to keep your bell jar clean is to wipe a thin film of
detergent over the surface of the clean glass and pump down to dry.
After each coating run simply rinse off the glass, the detergent comes
away and takes the coating with it. Recoat with detergent and dry.

Train ALL users in this technique, insist they clean the glass if they
"FORGOT" to do it after their last run, and you will always have a
clear, clean bell jar.
Regards
JVN
Rick Harris wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Well we've got a new vacuum evaporator and it has been so long since I've
} had one I've forgotten how to treat the bell jar to reduce the effort it
} takes to keep it clean. However, I do recall that there were little tricks
} to cleaning and treating the glass and other surfaces. Anyone care to shed
} a little light my darkened memory of these procedures?
}
} TIA
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} 530 754 7536 fax
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************


From daemon Tue Oct 10 22:06:21 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Tue, 10 Oct 2000 20:04:42 -0700 (PDT)
Subject: High Resolution SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I'm getting ready to purchase a new sputter coater for our FESEM. I'd
like to hear how lucky others were with using a "regular" sputter coater
for high resolution SEM work. Currently, there is a noise problem with
our SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm
ZrO2 close-packed particles, and 4nm pores in glass using a thin coating
of Au/Pd applied with an old Polaron. One thing I'm worried about is =
once the noise is fixed and I can resolve the published 1.5nm resolution
will I be able to resolve the grains in the coating?

Thanks,
Ben



#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \





From daemon Tue Oct 10 23:06:07 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 Oct 2000 00:06:57 -0500
Subject: Bell jar maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Rick A. Harris wrote:
===============================================================
Well we've got a new vacuum evaporator and it has been so long since I've
had one I've forgotten how to treat the bell jar to reduce the effort it
takes to keep it clean. However, I do recall that there were little tricks
to cleaning and treating the glass and other surfaces. Anyone care to shed
a little light my darkened memory of these procedures?
===============================================================
One product that will do this is called Bell Bright™. A companion product
for metal surfaces is called Metal Bright™. Both are described in detail
on the SPI website at URL
http://www.2spi.com/catalog/supp/supp3.html

The concept is to leave a thin water soluble polymer behind on the clean
glass (or metal) surface, and then when it is time to remove the evaporated
deposited, a lint free cotton wiper and water alone will wipe away most of
the material (as the originally deposited polymer film dissolves away).

Disclaimer: SPI Supplies "invented" this product in the late 1970's and has
offered it ever since. We would have a vested interest in seeing its use
increase.


Chuck


PS: Remember that we are trying to become 100% paperless and the only way
that we can manage this kind of correspondence, in a paperless environment,
is for our correspondents to always reply by way of "reply" on their
software so that the entire string of correspondence on that topic gets
returned to us, and the entire correspondence history can be kept in one
place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================


From daemon Wed Oct 11 00:24:27 2000



From: Chris Baker :      chris-at-collegewafer.com
Date: Wed, 11 Oct 2000 01:26:19 -0700
Subject: Si, MEMS, GaAs, SOI, InP, GaN, Ge, PtSi, SOI & Equipment..

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Visit http://www.collegewafer.com for your free quote, or call (800) 713-9375
or
Fax (888)-832-0340!!! E-mail us at sales-at-collegewafer.com

Quantities you want! Wafers in stock and ready to ship 12" Wafers available.

All Ultrathin Si wafers polished two sides with TTV { 3 microns for (MEMS) Applications.
4"N(100) 1-10 ohm-cm 10-100 microns polished two sides in Silicon!

New Platinum Silicon (PtSi)price posted!

IN STOCK & READY to SHIP
Si, GaAs, SOI, Ge, InP, InAs, InSb, GaSb, GaN in 1"-12" diameters.

Ultra-Thin Si available in the quantities you need.

Customer supplied GaAs or Ge can be reclaimed or repolished.

Equipment
Used Vacuum Deposition Equipment Evaporation Systems CHA/Ion Tech Milling Balzers
BAK760 and Spitfire & more! Visit us for details!

Contact us for your free quote today!

Also visit http://www.thecoveredcall.com learn to pick winning stocks using he
CANSLIM method of investing with a covered call twist!

To REMOVE yourself from this list please visit
http://www.collegewafer.com/contact_us/Remove/remove.html

MEMS6730




From daemon Wed Oct 11 05:51:12 2000



From: Emma Bonino :      emma.bonino-at-elezioniradicali.padova.com
Date: Wed, 11 Oct 2000 11:25:33 +0200
Subject: Toc toc. Sono Emma Bonino e la prego...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco
online che stiamo organizzando perche' anche cittadini come lei (che non
votino affatto radicale o che lo facciano, che non votino o non intendano
piu' votare) possano partecipare alle nostre decisioni, essere presenti o
rappresentati nei nostri organi dirigenti.

In vista delle elezioni politiche occorre tentare di allearsi con il Polo o
con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni
non democratiche? Quali Riforme istituzionali, politiche, del lavoro,
dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle
liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste
o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti
devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi"
come finora?

Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri
del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a
questo gioco, lo preannunci e registri cliccando qui:
http://www.radicali.it/p_register/

Mi scusi ancora. A presto.

Emma Bonino

PS Questa email e' stata inviata nel rispetto della legge
sulla privacy; se desidera maggiori informazioni o se intende
cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/



From daemon Wed Oct 11 05:52:10 2000



From: Emma Bonino :      emma.bonino-at-elezioniradicali.padova.com
Date: Wed, 11 Oct 2000 11:25:33 +0200
Subject: Toc toc. Sono Emma Bonino e la prego...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco
online che stiamo organizzando perche' anche cittadini come lei (che non
votino affatto radicale o che lo facciano, che non votino o non intendano
piu' votare) possano partecipare alle nostre decisioni, essere presenti o
rappresentati nei nostri organi dirigenti.

In vista delle elezioni politiche occorre tentare di allearsi con il Polo o
con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni
non democratiche? Quali Riforme istituzionali, politiche, del lavoro,
dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle
liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste
o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti
devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi"
come finora?

Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri
del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a
questo gioco, lo preannunci e registri cliccando qui:
http://www.radicali.it/p_register/

Mi scusi ancora. A presto.

Emma Bonino

PS Questa email e' stata inviata nel rispetto della legge
sulla privacy; se desidera maggiori informazioni o se intende
cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/



From daemon Wed Oct 11 06:21:17 2000



From: bk22k-at-fiberia.com
Date: Wed, 11 Oct 00 06:04:50 EST
Subject: opportunity knocks-are you home?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


THE PROGRAM
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MULTI-LEVEL MARKETING (MLM) has finally gained respectability.
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You may have heard this story before, but over the summer Donald
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The enclosed information is something I almost let slip through my
fingers. Fortunately, sometime later I re-read everything and gave
somethought and study to it. My name is Johnathon Rourke. Two years
ago, the corporation I worked at for the past twelve years down-sized and my
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to open my own business. Over the past year, I incurred many
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The economy was taking a toll on my business and I just couldn't seem
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FINANCIALLY!!!

In mid December, I received this program via e-mail. Six month's
prior to receiving this program I had been sending away for
information on various business opportunities. All of the programs I
received, in my opinion, were not cost effective. They were either
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for me to risk to see if they would work or not. One claimed that I would
make a million dollars in one year...it didn't tell me I'd have to write a
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But like I was saying, in December of 1997 I received this program. I
didn't send for it, or ask for it, they just got my name off a
mailing list.THANK GOODNESS FOR THAT!!! After reading it several times, to make
sure I was reading it correctly, I couldn't believe my eyes. Here was a MONEY
MAKING PHENOMENON. I could invest as much as I wanted to start,
without putting me further into debt. After I got a pencil and paper
and figured it out, I would at least get my money back. But like most
of you I was still a little skeptical and a little worried about the
legal aspects of it all. So I checked it out with the U.S. Post Office
(1-800-725-2161 24-hrs) and they confirmed that it is indeed legal! After
determining the program was LEGAL and NOT A CHAIN LETTER, I decided
"WHY NOT."

Initially I sent out 10,000 e-mails. It cost me about $15 for my time
on-line. The great thing about e-mail is that I don't need any money
for printing to send out the program, and because all of my orders
are fulfilled via e-mail, my only expense is my time. I am telling
you like it is I hope it doesn't turn you off, but I promised myself that I would not
"rip-off" anyone, no matter how much money it made me.

In less than one week, I was starting to receive orders for REPORT #1
By January 13, I had received 26 orders for REPORT #1. Your goal is to
"RECEIVE at least 20 ORDERS FOR REPORT #1 WITHIN 2 WEEKS. IF
YOU DON'T, SEND OUT MORE PROGRAMS UNTIL YOU DO!" My first
step in making $50,000 in 90 days was done. By January 30, I had received
196 orders for REPORT #2. Your goal is to "RECEIVE AT LEAST 100+ ORDERS
FOR REPORT #2 WITHIN 2 WEEKS. IF NOT, SEND OUT MORE PROGRAMS
UNTIL YOU DO. ONCE YOU HAVE 100 ORDERS,
THE REST IS EASY, RELAX, YOU WILL MAKE YOUR $50,000 GOAL." Well, I
had 196 orders for REPORT #2, 96 more than I needed. So I sat back
and relaxed. By March 1, of my e-mailing of 10,000, I received $58,000 with
more coming in every day.

I paid off ALL my debts and bought a much needed new car. Please take
time to read the attached program, IT WILL CHANGE YOUR LIFE FOREVER!!
! Remember, it won't work if you don't try it. This program does work
, but you must follow it EXACTLY! Especially the rules of not trying
to place your name in a different place. It won't work and you'll
lose out on a lot of money!
In order for this program to work, you must meet your goal of 20+
orders for REPORT #1, and 100+ orders for REPORT #2 and you will make $50,000
or more in 90 days. I AM LIVING PROOF THAT IT WORKS!!!

If you choose not to participate in this program, I am sorry. It
really is a great opportunity with little cost or risk to you. If you
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to financial security. If you are a fellow business owner and are in
financial trouble like I was, or you want to start your own business, consider
this a sign. I DID!

Sincerely,
Johnathon Rourke

A PERSONAL NOTE FROM THE ORIGINATOR OF THIS PROGRAM:

By the time you have read the enclosed program and reports, you
should have concluded that such a program, and one that is legal,
could not have been created by an amateur.

Let me tell you a little about myself. I had a profitable business
for 10 years. Then in 1979 my business began falling off. I was doing
the same things that were previously successful for me, but it wasn't
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Inflation and recession had replaced the stable economy that had been
with us since 1945.I don't have to tell you what happened to the
unemployment rate... because many of you know from first hand
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The middle class was vanishing. Those who knew what they were doing
invested wisely and moved up. Those who did not, including those who
never had anything to save or invest, were moving down into the ranks
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GET POORER." The traditional methods of making money will never allow
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BIT OF EFFORT." You can make more money in the next few months than you
have ever imagined. I should also point out that I will not see a penny of this
money, nor anyone else who has provided a testimonial for this
program. I have already made over 4 MILLION DOLLARS!I have retired
from the program after sending thousands and thousands of programs.

Follow the program EXACTLY AS INSTRUCTED. Do not change it in any way
. It works exceedingly well as it is now. Remember to e-mail a copy
of this exciting report to everyone you can think of. One of the
people you send this to may send out 50,000...and your name will be on everyone of
them!

Remember though, the more you send out the more potential customers
you will reach.

So my friend, I have given you the ideas, information, materials and
opportunity to become financially independent. IT IS UP TO YOU NOW!

"THINK ABOUT IT"

Before you delete this program from your mailbox, as I almost did,
take a little time to read it and REALLY THINK ABOUT IT. Get a pencil
and figure out what could happen when YOU participate. Figure out the
worst possible response and no matter how you calculate it, you will
still make a lot of money! You will definitely get back what you
invested. Any doubts you have will vanish when your first orders come
in. IT WORKS!

Jody Jacobs, Richmond, VA

HERE'S HOW THIS AMAZING PROGRAM WILL MAKE YOU THOUSANDS OF
DOLLAR$

INSTRUCTIONS:

This method of raising capital REALLY WORKS 100% EVERY TIME.
I am sure that you could use up to $50,000 or more in the next 90
days. Before you say "BULL... ", please read this program carefully.

This is not a chain letter, but a perfectly legal money making
opportunity. Basically, this is what you do: As with all multi-level
businesses, we build our business by recruiting new partners and
selling our products. Every state in the USA allows you to recruit
new multi-level business partners,
and we offer a product for EVERY dollar sent. YOUR ORDERS COME BY
MAIL AND ARE FILLED BY E-MAIL, so you are not involved in personal
selling. You do it privately in your own home, store or office. This
is the GREATEST Multi-Level Mail Order Marketing anywhere.

This is what you MUST do:

1. Order all 4 reports shown on the list below (you can't sell them
if youdon't order them).
-- For each report, send $5.00 CASH, the NAME & NUMBER OF THE REPORT
YOU ARE ORDERING, YOUR E-MAIL ADDRESS, and YOUR NAME & RETURN
ADDRESS (in case of a problem) to the person whose name appears on
the list next to the report. MAKE SURE YOUR RETURN ADDRESS IS ON
YOUR ENVELOPE IN CASE OF ANY MAIL PROBLEMS!
-- When you place your order, make sure you order each of the four
reports. You will need all four reports so that you can save them on
your computer and resell them.
-- Within a few days you will receive, via e-mail, each of the four
reports. Save them on your computer so they will be accessible for you to send
to the 1,000's of people who will order them from you.

2. IMPORTANT DO NOT alter the names of the people who are listed next
to each report, or their sequence on the list, in any way other than
is instructed below in steps "a" through "f" or you will lose out on
the majority of your profits. Once you understand the way this works,
you'll also see how it doesn't work if you change it. Remember, this
method has been tested,and if you alter it, it will not work.
a. Look below for the listing of available reports.
b. After you've ordered the four reports, take this advertisement and
remove the name and address under REPORT #4. This person has
made it through the cycle and is no doubt counting their $50,000!
c. Move the name and address under REPORT #3 down to REPORT #4.
d. Move the name and address under REPORT #2 down to REPORT #3.
e. Move the name and address under REPORT #1 down to REPORT #2.
f. Insert your name/address in the REPORT #1 position.

Please make sure you COPY ALL INFORMATION, every name and
address,
ACCURATELY!

3. Take this entire letter, including the modified list of names, and
save it to your computer. Make NO changes to the instruction portion
of this letter.

Your cost to participate in this is practically nothing (surely
you can afford $20). You obviously already have an Internet
connection and e-mail is FREE!



There are two primary methods of building your downline:

METHOD #1: SENDING BULK E-MAIL

Let's say that you decide to start small, just to see how it goes,
and we'll assume you and all those involved send out only 2,000
programs each. Let's also assume that the mailing receives a 0.5%
response. Using a good list the response could be much better. Also,
many people will send out hundreds of
thousands of programs instead of 2,000. But continuing with this
example, you send out only 2,000 programs. With a 0.5% response, that
is only 10 orders for REPORT #1. Those 10 people respond by sending
out 2,000 programs each for a total of 20,000. Out of those 0.5%, 100
people respond and order REPORT #2. Those 100 mail out 2,000 programs
each for a total of 200,000.
The 0.5% response to that is 1,000 orders for REPORT #3. Those 1,000
send out 2,000 programs each for a 2,000,000 total. The 0.5% response
to that is 10,000 orders for REPORT #4. That's 10,000 $5 bills for
you. CASH!!! Your total income in this example is $50 + $500 + $5,000
+ $50,000 for a total of
$55,550!!! REMEMBER FRIEND, THIS IS ASSUMING 1,990 OUT OF THE 2,000
PEOPLE YOU MAIL TO WILL DO ABSOLUTELY NOTHING AND TRASH THIS
PROGRAM! DARE TO THINK FOR A MOMENT WHAT WOULD HAPPEN IF
EVERYONE, OR HALF SENT OUT 100,000 PROGRAMS INSTEAD OF 2,000.
Believe me, many people will do justthat, and more! By the way, your cost to
participate in this is practically nothing. You obviously already have an Internet
connection and e-mail is FREE!!! REPORT #2 will show you the best
methods for bulk e-mailing, tell you where
to obtain free bulk e-mail software and where to obtain e-mail lists.



METHOD #2 - PLACING FREE ADS ON THE INTERNET

Advertising on the internet is very, very inexpensive, and there are
HUNDREDS of FREE places to advertise. Let's say you decide to start
small just to see how well it works. Assume your goal is to get ONLY
10 people to participate on your first level. (Placing a lot of FREE
ads on the Internet will EASILY get a larger response.) Also assume that
everyone else in YOUR ORGANIZATION gets ONLY 10 downline members.
Follow this example to achieve the STAGGERING results below:

1st level--your 10 members with $5.......................................$50
2nd level--10 members from those 10 ($5 x 100)..................$500
3rd level--10 members from those 100 ($5 x 1,000)...........$5,000
4th level--10 members from those 1,000 ($5 x 10,000).....$50,000
THIS TOTALS ----------} $55,550

Remember friends, this assumes that the people who participate only
recruit 10 people each. Think for a moment what would happen if they
got 20 people to participate! Most people get 100's of participants!
THINK ABOUT IT! For every $5.00 you receive, all you must do is e-mail them
the report they ordered. THAT'S IT! ALWAYS PROVIDE SAME-DAY SERVICE
ON ALL ORDERS! This will guarantee that the e-mail THEY send out with YOUR
name and address on it will be prompt because they can't advertise
until they receive the report!

AVAILABLE REPORTS

*** Order Each REPORT by NUMBER and NAME ***
Notes:
-- ALWAYS SEND $5 CASH (U.S. CURRENCY) FOR EACH REPORT. CHECKS NOT
ACCEPTED.
-- ALWAYS SEND YOUR ORDER VIA FIRST CLASS MAIL.
-- Make sure the cash is concealed by wrapping it in at least two
sheets of paper. On one of those sheets of paper, include:
(a) the number & name of the report you are ordering, (b) your
e-mail address, and (c) your name & postal address.

PLACE YOUR ORDER FOR THESE REPORTS NOW:


REPORT #1 "The Insider's Guide to Advertising for Free on the
Internet"


ORDER REPORT #1 FROM:

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Remove at qmkx-at-netscape.net
/////////////////////////////////////////////////////////////////









From daemon Wed Oct 11 06:29:10 2000



From: Emma Bonino :      emma.bonino-at-elezioniradicali.padova.com
Date: Wed, 11 Oct 2000 11:25:33 +0200
Subject: Toc toc. Sono Emma Bonino e la prego...

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Wed, 11 Oct 2000 11:22:01 +0200
Message-ID: {001401c03365$51216370$2c9b83d4-at-pol.it}
Reply-To: "Emma Bonino" {emma.bonino-at-elezioniradicali.padova.com}


Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco
online che stiamo organizzando perche' anche cittadini come lei (che non
votino affatto radicale o che lo facciano, che non votino o non intendano
piu' votare) possano partecipare alle nostre decisioni, essere presenti o
rappresentati nei nostri organi dirigenti.

In vista delle elezioni politiche occorre tentare di allearsi con il Polo o
con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni
non democratiche? Quali Riforme istituzionali, politiche, del lavoro,
dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle
liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste
o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti
devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi"
come finora?

Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri
del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a
questo gioco, lo preannunci e registri cliccando qui:
http://www.radicali.it/p_register/

Mi scusi ancora. A presto.

Emma Bonino

PS Questa email e' stata inviata nel rispetto della legge
sulla privacy; se desidera maggiori informazioni o se intende
cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/



From daemon Wed Oct 11 06:39:50 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Wed, 11 Oct 2000 21:09:07 +1000
Subject: RE: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
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It all depends: how busy you are, how expensive staff time in your country is
and how costly the place is you buy your stubs from. Obviously some specimens
should be stored in a desiccator for an eternity and other can be discarded
immediately.
There is nothing magical about cleaning stubs. Solvents are messy. Best to
place on some newspaper a very coarse file or a sheet of coarse (50 grit)
sandpaper and then rub the old stub over that file of sandpaper until specimen
and glue are removed.

Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this
leaves some money for useful purchases.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori
[SMTP:sojilori-at-oauife.edu.ng] wrote:
}
} Hello Guys
} I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} just installed in our central science lab.
} Is there a way to clean stubs for reuse or are stubs disposable once used.
} ( This may sound naive)
} soji
} Mr. O. O. ILORI
} DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} OBAFEMI AWOLOWO UNIVERSITY,
} ILE-IFE, OSUN STATE
} NIGERIA.
} email: sojilori-at-oauife.edu.ng
}
}



From daemon Wed Oct 11 07:33:25 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 11 Oct 2000 08:57:04 -0400
Subject: Inquiry re: assessment of critical point dryers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
The following are responses to my inquiry about
automated critical point dryers. There was one other which
I will post as well. Thanks to all who responded.
Rosemary

I find automated CPDs easier only in the sense that they make
training new users easier. But they also can encourage not paying
sufficient attention to the drying instrument. I like the Polaron, in
particular it's large chamber. Let's see ... the only other advantage
to an automated system is if you have lots of samples to do in lots
of runs. Otherwise, I've never seen enough of an advantage to justify
the extra cost.

But! having said that, a CPD with autobleed is an excellent choice.
This is a good safety feature, and can help protect specimens.

Phil


There are safety considerations, that is one of the several reasons why
there is such a preference for the E3000 or E3100. BTW another good
thing about the Polaron design and larger chamber is the
less likelihood of turbulence, or putting it another way, you can do your
exchanges at a faster rate because you can use larger flow rates before
turbulence does set in.

Chuck (Garber)

We recently purchased a Tousimis
795 semi-automatic critical point
dryer for our multi-user facilty.
This unit replaces an old Denton
"bomb". We considered ease of use
for our customers to be an
important consideration. Our unit
has preset purge, bleed, vent and
heating features which make it
easier to use. We don't have to lug
a large beaker of hot water over to
the CPD to heat the chamber. The
flow rates are factory preset but
easily adjustable. Having these
features, I believe, allows us to
produce more consistent,
reproducible results.
The standard chamber size is rather
small but a chamber extension is
available and easy to install. We
have found that when trying to
critical point dry very large
specimens, the chamber size can be
a problem (most of our work is
Drosophila flies or mouse embryos).

Although very few people do their
own critical point drying here, our
goal is to make the facility as
user-friendly as possible, and this
seemed to be a step in that
direction.

Happy hunting.

Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging
Facility

Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html




From daemon Wed Oct 11 08:04:38 2000



From: Nagy Peter :      nagyp-at-chemres.hu
Date: Wed, 11 Oct 2000 08:02:17 -0500
Subject: Backscattered

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Collegues,

i'd like to purchase a backscattered detector for an old Hitachi SEM.
I am looking for vendors of such equipment, as Robinson detector, KE Si
detector and so on. Could you help me with some addresses?

Thanks,

Peter

--
Dr NAGY Peter
Chemical Research Center
Institute of Chemistry of the Hungarian Academy of Sciences
1025 Budapest Pusztaszeri ut 59-67.
Phone: 36-1-325-7548
Fax: 36-1-325-7509
E-mail: nagyp-at-chemres.hu




From daemon Wed Oct 11 08:29:02 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 11 Oct 2000 14:23:13 +0000
Subject: RE: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Soji
We usually treat the standard aluminium LEO pin stubs as
disposable. Since we have a number of users each with a different
specimen type and mounting procedure it becomes difficult to
identify a single effective cleaning method, and staff time is more
costly than the stubs.

If you are a novice user you may not yet realise that LEO pin stubs
are obtainable at a fraction of LEO's price from most microscopy
supply houses.

http://www.kaker.com/mvd/list.html
is a list of microscopy vendors which you may find useful. Use this
to look up web sites of some of the following companies: Agar
Scientific, TAAB Laboratories, SPI, Electron Microscopy Science,
etc. etc.

However, cleaning stubs can be cost-effective if you routinely
produce many tens or hundreds of specimens in exactly the same
way. If you have to do this it pays to find adhesives and conductive
paints that redissolve readily in the same solvent, so things like
epoxies are out. Choose your solvents carefully, because some
like ethanol attack aluminium if it is immersed for long periods.

Another approach which avoids the need to clean stubs is to mount
up your specimens on thin squares or discs of metal (e.g. thin
aluminium sheet) which are then tacked to the stub using e.g.
graphite dag. After use the metal supports can be cracked off and
replaced with new ones. This way the stub can be re-used more
often before it needs to be properly cleaned up.
Good luck
Chris

} On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori
} [SMTP:sojilori-at-oauife.edu.ng] wrote:
} }
} } Hello Guys
} } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} } just installed in our central science lab.
} } Is there a way to clean stubs for reuse or are stubs disposable once used.
} } ( This may sound naive)
} } soji
} } Mr. O. O. ILORI
} } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} } OBAFEMI AWOLOWO UNIVERSITY,
} } ILE-IFE, OSUN STATE
} } NIGERIA.
} } email: sojilori-at-oauife.edu.ng
} }
} }
}
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Wed Oct 11 09:30:46 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Wed, 11 Oct 2000 07:24:33 -0700
Subject: Bacterial quantitation in soil samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


(resubmitting message from Mon 9/10)


We are considering a purchase of a system for quantitative analysis of bacterial
and fungal biovolume in soil samples. Could anyone provide info on preferred
method (FITC labeling?) and equipment. As always, I appreciate your help,
Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321




From daemon Wed Oct 11 09:34:09 2000



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 11 Oct 2000 09:28:26 -0500
Subject: RE: BSE Detector systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Ref: Diode detector type...
I have used GW Electronics. First on an ETEC SEM, and now on a Hitachi
S-3500. I started with a model (many years ago) which is now discontinued
and have upgraded to the current design. Both worked well for me. Check:
http://www.gwelectronics.com/

----------------------------------------
Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

Me: http://home.att.net/~woody.white


From daemon Wed Oct 11 09:46:34 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 11 Oct 2000 08:10:01 -0500
Subject: Re: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
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Peter
Try looking at this site.
http://www.kaker.com/mvd/list.html
It contains links to dozens of microscopy vendor sites
Chris

Date sent: Wed, 11 Oct 2000 08:02:17 -0500
To: Microscopy-at-sparc5.microscopy.com
} From: Nagy Peter {nagyp-at-chemres.hu}


The last time this subject came up, someone suggested wiping the inside of
the jar with Polaroid print coaters. They seem to provide a water soluble
layer that easily comes off. We used to have dozens of extras lying around
and now had a use for them. However, now that we have largely gone to
digital imaging we will have to watch our stock.

At 04:49 PM 10/10/2000 -0700, you wrote:

} Well we've got a new vacuum evaporator and it has been so long since I've
} had one I've forgotten how to treat the bell jar to reduce the effort it
} takes to keep it clean. However, I do recall that there were little
} tricks to cleaning and treating the glass and other surfaces. Anyone care
} to shed a little light my darkened memory of these procedures?

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking


From daemon Wed Oct 11 10:25:33 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Wed, 11 Oct 2000 11:22:39 -0400
Subject: Re: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
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We agree with John Nailon that if a user forgets to prepare the
evaporator prior to a run he should have to clean it.
Since we have to clean our evaporators several times a day because of
our substrate production, we do the following.

1. Place Victawet in a medium basket.
2. Evaporate the Victawet at 10(-5). This coats not only the bell jar,
but all the other surfaces under the bell jar as well.
3. Clean the system with windex (or water) and wipe with a lint free
cloth.
4. Re-coat with victawet prior to the next run.

John Arnott

Disclaimer: In additon to using, Ladd has been selling most of the above
mentioned products, including Victawet, Lint-free cloth, Substrates,
Evaporators and Baskets since 1955.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955


John Nailon wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} The easiest way to keep your bell jar clean is to wipe a thin film of
} detergent over the surface of the clean glass and pump down to dry.
} After each coating run simply rinse off the glass, the detergent comes
} away and takes the coating with it. Recoat with detergent and dry.
}
} Train ALL users in this technique, insist they clean the glass if they
} "FORGOT" to do it after their last run, and you will always have a
} clear, clean bell jar.
} Regards
} JVN
} Rick Harris wrote:
} }
} } ------------------------------------------------------------------------} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.} }
} } Well we've got a new vacuum evaporator and it has been so long since I've
} } had one I've forgotten how to treat the bell jar to reduce the effort it
} } takes to keep it clean. However, I do recall that there were little tricks
} } to cleaning and treating the glass and other surfaces. Anyone care to shed
} } a little light my darkened memory of these procedures?
} }
} } TIA
} }
} } Rick A. Harris, Director
} } Microscopy and Imaging Facility
} } Section of Molecular and Cellular Biology
} } 1241 Life Sciences Addition
} } University of California
} } Davis, CA
} } 530 752 2914
} } 530 754 7536 fax
} } http://katie.ucdavis.edu
} } raharris-at-ucdavis.edu
}
} --
} ****************************************************
} John V Nailon
} Operations Manager
} Centre for Microscopy and Microanalysis
} The University of Queensland
} St. Lucia Queensland 4072
} Phone: +61-7-3365-4214
} Fax: +61-7-3365-4422
} WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
} ****************************************************


From daemon Wed Oct 11 10:38:06 2000



From: Joel McClintock :      jmcclin-at-pop.uky.edu
Date: Wed, 11 Oct 2000 23:36:54 -0400
Subject: Recoat Phosphor Screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

I need to have the phosphor screens recoated on an EM 400T we have on
campus. I have been told that Grant Scientific does a good job. I have not
been able to find a way to contact them. Can anyone help with a phone number
or info.? I would also welcome any other suggestions. Thanks.

Joel McClintock
EM Specialist
Univ. of Kentucky
Lexington, Ky
859-257-1242



From daemon Wed Oct 11 10:50:42 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 11 Oct 00 10:48:02 -0600
Subject: Re: Bell Jar Cleaning:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


To minimize the area which needs cleaning, I obtained a pyrex glass
cylinder large enough to fit vertically over my evaporating supports,
etc. The ends of it were coated with a vacuum rated epoxy to prevent
chipping.
Before using, it is coated with a commercial release agent made for this
"easy
cleaning" purpose. The bottom sits on a sheet of disposable aluminum foil
and
the top is also covered by aluminum foil. To facilitate pumping, a 1/4"
thick
scrap of ceramic placed under the bottom edge of the "containment
cylinder"
aids pumping at a reasonable rate and provides a path for one of the
power
leads for my evaporating basket, insulating the lead from a metal support

platform. After use, the foil can be scrapped and only a 6" diameter
cylinder
requires cleaning!

Bernie Kestel
Materials Science Division
Argonne National Laboratory
9700 South Cass Avenue
Argonne, Il., 60439

E-mail: {kestel-at-anl.gov}



From daemon Wed Oct 11 10:54:31 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 11 Oct 2000 08:51:37 -0700
Subject: Re: bell jar treatment

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Dear Rick,
I used to use Victawet and it was the best, but it is not available, so I
use a bar of hand soap. After you clean the bell jar or, if it is new, after
you wet it, rub the dry bar of soap all over the inside and rub with a paper
towel to spread it evenly and make it transparent. Good luck.
At 04:49 PM 10/10/00 -0700, you wrote:
} Well we've got a new vacuum evaporator and it has been so long since I've
} had one I've forgotten how to treat the bell jar to reduce the effort it
} takes to keep it clean. However, I do recall that there were little tricks
} to cleaning and treating the glass and other surfaces. Anyone care to shed
} a little light my darkened memory of these procedures?
}
} TIA
}
} Rick A. Harris, Director
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Oct 11 11:03:42 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 11 Oct 2000 08:56:48 -0700
Subject: Re: SEM: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Olori,
The stubs used for SEM are reused for years, but the grids for a TEM are
disposed of. I clean my SEM stubs by rubbing on a paper towel wet with
either lab alcohol (ethanol) or acetone, depending what was on them. If I am
using the carbon double-side sticky tabs, I soak the stubs in acetone for an
hour, then rub on a paper towel to remove the tab. Congratulations on your
new SEM.
At 06:09 PM 10/10/00 +0100, you wrote:
}
} Hello Guys
} I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} just installed in our central science lab.
} Is there a way to clean stubs for reuse or are stubs disposable once used.
} ( This may sound naive)
} soji
} Mr. O. O. ILORI
} DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} OBAFEMI AWOLOWO UNIVERSITY,
} ILE-IFE, OSUN STATE
} NIGERIA.
} email: sojilori-at-oauife.edu.ng

Good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Oct 11 11:19:14 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 Oct 2000 12:19:51 -0500
Subject: BSE detector vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Peter Nagy wrote:
==========================================================
i'd like to purchase a backscattered detector for an old Hitachi SEM. I am
looking for vendors of such equipment, as Robinson detector, KE Si detector
and so on. Could you help me with some addresses?
===========================================================
Try
Microscopy Vendor's Data Base
http://207.137.96.185/mvd/products/back.html

They have them all, I think.



Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================


From daemon Wed Oct 11 11:36:05 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 11 Oct 2000 11:33:08 -0500
Subject: Re: digital imaging info needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I suppose that John Russ and others have stated the matter concisely and
eloquently in their books and articles on the subject, but the concepts are
not that difficult that I will take a stab at it.

A digital image is nothing more than a two-dimensional array of numbers
where the numbers represent some characteristic of the image. Various image
sources vary in the way that the arrays are assembled and what the numbers
represent.


In an scanning electron microscope or a scanning probe microscope (which I
suppose you could say that an SEM is), a single detector element is used to
provide the signal at all points in the image. The X and Y coordinates of
the scan are used to determine the array or pixel index, and the signal is
digitized at each point.

Many systems utilize "active" control where the beam is directed to each
coordinate in succession under computer control. A digital grid is
predetermined within the computer and the coordinates are fed out through
appropriate hardware to convert the digital coordinates to the necessary
analog voltage to scan the probe to the desired point. A microscope signal
is measured at that point and digitized for storage in the image array. The
scan speed and resolution are under direct control of a computer.

Other systems are termed "passive" since the existing analog microscope
scan system is used to control the probe scan as normal. The digitizing
system monitors the x and y coordinates and records the signal level when
the beam is in positions corresponding to the pixels in the image array.

There are a variety of signals available from electron microscopes.
Detectors for secondary electrons and backscattered electrons operate as
analog devices. (One might argue that since they are counting a finite
number of electrons, then they could be setup as digital devices. However,
the count rate is high enough that digital detectors are probably not
feasible, and even if they were there remains a question of when to start
counting.) A variety of detectors and devices are used (including PMTs -
photo multiplier tubes) to measure the signal, but they are primarily, if
not all, analog devices. An analog to digital convertor is used to convert
the signal to digital for the image array. Some scopes may digitize the
signal early on so that no analog signal is readily available, but it still
exists somewhere inside the instrument.

X-ray signals in an electron microscope are probably treated as digital
from the outset. Since the x-ray photons are detected as pulses from the
x-ray detector, they are treated as discrete events and counted. Since
counting is involved, x-ray signals are only used with active control
systems (to the best of my knowledge). A counter is reset each time the
probe is moved to a new location.


There is a whole other class of source for digital images. In this class
there is a separate detector for each element of the array. The entire
image is present at once as opposed to being built up by a scanned probe.
The detectors operate in parallel as they build up and digitize the signal
simultaneously. At some point the signal is read and the detectors are
reset. CCD cameras are the most common device in this category. They find
application in light microscopes and transmission electron microscopes as
well as in consumer electronics such as video cameras and still cameras.

In these systems, the images are digital from the start. The readout is in
terms of an electron count. It usually gets scaled to another digital range
(0-255, 0-4095, etc) so that the original digital count may no longer be
significant.

The size of the image array is determined by the size of the CCD array.
Smaller image sizes can be obtained by grouping the signals from adjacent
elements in the CCD array. Larger images are sometimes manufactured by
interpolating the signals of neighboring elements, but that is not
introducing new information.


I hope this helps. Feel free to ask for further clarification.

Warren S.

At 06:40 PM 10/10/2000 -0400, you wrote:
} Dear listserv:
}
} Dee Breger was kind enough to forward this message for me. I'm writing a book
} on digital photography, to be published by Harry N. Abrams next year. The book
} will include a section on applied digital imaging, and I am particularly
} interested in scientific imaging. I am hoping that some of you folks might
} be able
} to provide some information.
}
} For the purposes of the book, I am defining digital imaging as that which
} describes a scene using discrete digital information in a raster grid, or
} bitmap. So, I am particularly interested in scientific imaging applications
} which gather information in this way.
}
} I would be interested to hear if there are other scientific imaging systems
} (DEE: besides SEMs) which collect information in this manner. For instance,
} it sounds as though atomic force microscopes work in this way: as they
} drag a stylus across a surface, the position is recorded (x and y), along
} with the height (z). This
} is later used to re-create the image in a raster grid (the fact that the AFM
} does not use light makes it another interesting foil to 'traditional' digital
} photography). How then, are colors in the final output determined from the
} data collected? I would also, of course, love to see any images you might have
} to show.
}
} Thanks in advance,
} Jonathan Lipkin (jlipkin-at-ramapo.edu)
}
} DEE: I'll forward to Jonathan any replies that are publically posted to the
} listserver. He also needs other detailed technical information on digital
} image formation which can be far more comprehensively clarified and
} amplified by some of the experts among my fellow listers than by me, though
} I've already given him the broad picture for analog scopes like my dear old
} Cambridge 250. His SEM impressions, which I'll pass along verbatim,
} include (1) "SEMs collect information, used to fill a raster grid, through
} the use of a PMT", (2) the signal is sent directly to the crt in analog
} form -I always had thought that the pmt sent out digital info, but it
} sounds like it just sends out analog data (a waveform? though it couldn't
} be a waveform if the raster grid is discrete in two dimensions)" and (3)
} "as the secondary electrons come off the sample, they hit a detector which
} generates a digital number (redundant) which is then placed in a pixel of a
} bitmap."
}
} Many thanks to any who can help!

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking


From daemon Wed Oct 11 12:11:19 2000



From: A. Greene :      ablue-at-io.com
Date: Wednesday, October 11, 2000 11:45 AM
Subject: Recoat Phosphor Screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello Joel,

Grant does very fine work. As a mater of fact they are the source used by a
few OEMs.
Their address is 1385 Rock Island Road
Gilbert South Carolina 29054
Phone: 803/892-2841

Regards,

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone: 512/282-5507 Fax: 512/280-0702

QUALITY ELECTRON MICROSCOPE REPAIR
-----Original Message-----
} From: Joel McClintock {jmcclin-at-pop.uky.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Wed Oct 11 12:16:42 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 11 Oct 2000 18:08:26 +0100 (GMT Daylight Time)
Subject: Re: RE: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


We slice off the specimen with a razor blade and use the
blade to scrape the stub fairly clean. As we use colloidal
graphite, which is conductive, I do not mind some residual
glue as it will get covered by glue from the next use.
This glue can be removed, if required, by sonicating in
butyl acetate.

Dave


On Wed, 11 Oct 2000 21:09:07 +1000 Jim at ProSciTech
{jim-at-proscitech.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} It all depends: how busy you are, how expensive staff time in your country is
} and how costly the place is you buy your stubs from. Obviously some specimens
} should be stored in a desiccator for an eternity and other can be discarded
} immediately.
} There is nothing magical about cleaning stubs. Solvents are messy. Best to
} place on some newspaper a very coarse file or a sheet of coarse (50 grit)
} sandpaper and then rub the old stub over that file of sandpaper until specimen
} and glue are removed.
}
} Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this
} leaves some money for useful purchases.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori
} [SMTP:sojilori-at-oauife.edu.ng] wrote:
} }
} } Hello Guys
} } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} } just installed in our central science lab.
} } Is there a way to clean stubs for reuse or are stubs disposable once used.
} } ( This may sound naive)
} } soji
} } Mr. O. O. ILORI
} } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} } OBAFEMI AWOLOWO UNIVERSITY,
} } ILE-IFE, OSUN STATE
} } NIGERIA.
} } email: sojilori-at-oauife.edu.ng
} }
} }
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Oct 11 12:30:47 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 11 Oct 2000 13:26:36 -0400
Subject: Re: Recoat Phosphor Screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html






I need to have the phosphor screens recoated on an EM 400T we have on
campus. I have been told that Grant Scientific does a good job. I have not
been able to find a way to contact them. Can anyone help with a phone number
or info.? I would also welcome any other suggestions. Thanks.
Dear Joel,
(803)892-2841. I just tried it, and it works. I also think that Grant did
a good job for us.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Wed Oct 11 12:32:51 2000



From: Rosanne Healy :      rhealy-at-iastate.edu
Date: Wed, 11 Oct 2000 12:29:39 -0500
Subject: infiltration of leaf/jet freeze fix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi List,
This is a question/situation a colleague of mine wanted to post-her
email for replies not on the list is rhealy-at-iastate.edu.
Of all people out there, I know you all can help!
Thanks so much!
Tracey Pepper

We are working with Cochliobolus inoculated maize leaf tissue using jet-propane
fixation, and freeze substitution using acetone (for immuno resins we switch to
ultra pure ETOH prior to infiltration), and have attempted using several
immuno-type resins such as: Lowicryl HM20 (with poly. at -20 under UV), LR
White with accel. at 4deg., and Quetol for general morphology. Our ultimate
goal is to visualize extra-cellular matrix of fungus within leaf tissues using
immunocytochem. So far, fixation is great (general morph.) However, the
immuno resins are not infiltrating adequately (under -30 deg.for Lowicryl and
4 deg. for LR White), lots of separation, and shattering. The longest we have
infiltrated has been 1-2 hr. each step for LR White. The Lowicryl had an
overnight in pure followed by 1/2 day for each of five total changes.
Any and all suggestions would be greatly appreciated!
Regards,
Rosanne Healy



Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Wed Oct 11 12:43:05 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 Oct 2000 13:39:52 -0500
Subject: Coater for use with FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ben Craft wrote:
=============================================================
I'm getting ready to purchase a new sputter coater for our FESEM. I'd
like to hear how lucky others were with using a "regular" sputter coater
for high resolution SEM work. Currently, there is a noise problem with our
SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm ZrO2
close-packed particles, and 4nm pores in glass using a thin coating of Au/Pd
applied with an old Polaron. One thing I'm worried about is = once the noise
is fixed and I can resolve the published 1.5nm resolution will I be able to
resolve the grains in the coating?
=============================================================
One system to consider is the Osmium Plasma Coater OPC-60 made by Nippon
Laser and Electronics, Nagoya, Japan. It is distributed by SPI Supplies.
You can find extensive information and examples on URL
http://www.2spi.com/catalog/osmi-coat.html

The end result is an amorphous coating so there is no grain size with which
one has to contend. The source of the osmium is an ampoule of osmium
tetroxide. This is not just a simple matter of sputtering from an osmium
metal cathode. And since the coating is an inert precious group metal, its
shelf life is quite long, if not infinite. Safety concerns are address on
the webpage mentioned above.

Contact me off-line should you want us to do a demo coating for you.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================


From daemon Wed Oct 11 13:34:00 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Wed, 11 Oct 2000 11:27:52 -0700 (PDT)
Subject: questions for Pelco CPD2 users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers

We just bought a Pelco CPD2 with the hopes of replacing our old Polaron
E3000, and so far, we're rather unimpressed just trying to get it through
an initial setup.
We were wondering if any other users could tell us how many runs they get
out of an average CO2 cylinder? The cooling process with the CO2 seems
somewhat inefficient at this point and we're using up a lot just trying to
cool the chamber. Also any recommendations on the soak vs. the rinse
methods of infiltrating the samples would be appreciated.
I can only assume the operations get a little easier with practise, but
right now we're wondering whether it's worth the investment in the long
run. The Polaron has been very reliable and has served us well, but we're
worried that its age is compromising its safety.

Thanks in advance,

Pauline Yu
splene-at-pw.usda.gov
Microscopist Technician
USDA-ARS-WRRC



From daemon Wed Oct 11 14:44:04 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 11 Oct 2000 14:38:56 -0500
Subject: Poster-sized Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Our microscopy facility recently merged with the scientific
photography and graphics production unit on campus.
We need to quickly evaluate the potential value of a large format
inkjet printer for producing posters for scientific meetings.

We welcome comments, suggestions and especially personal experiences
using inkjet technology for poster production. Is it worth the
expense, do researchers use it, etc... We are looking at the Epson
9000 printer in combination with the Fujifilm digital 9000 Photo
Graphix RIP. Good idea? How about software for poster production?

Also, vendors are welcome to contact me and to submit quotations. We
need all of this information by Friday (Oct 13). Sorry for the rush
but I just now got the word that funds may be available. Whew....

Many thanks....


John B.

--
####################################################################
John J. Bozzola, Ph.D., Director
IMAGE (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################


From daemon Wed Oct 11 15:29:26 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Wed, 11 Oct 2000 16:26:14 -0400
Subject: Re: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mary Mager,

Victawet is a stock item for Ladd. We sell and use it on a daily basis.
Please let me know off-line if you want a quote.

Thanks,

JD Arnott


--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955
Mary Mager wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Dear Rick,
} I used to use Victawet and it was the best, but it is not available, so I
} use a bar of hand soap. After you clean the bell jar or, if it is new, after
} you wet it, rub the dry bar of soap all over the inside and rub with a paper
} towel to spread it evenly and make it transparent. Good luck.
} At 04:49 PM 10/10/00 -0700, you wrote:
} } Well we've got a new vacuum evaporator and it has been so long since I've
} } had one I've forgotten how to treat the bell jar to reduce the effort it
} } takes to keep it clean. However, I do recall that there were little tricks
} } to cleaning and treating the glass and other surfaces. Anyone care to shed
} } a little light my darkened memory of these procedures?
} }
} } TIA
} }
} } Rick A. Harris, Director
} Regards,
} Mary
}
} Mary Mager
} Electron Microscopist
} Metals and Materials Engineering
} University of British Columbia
} 6350 Stores Road
} Vancouver, B.C. V6T 1Z4
} CANADA
} tel: 604-822-5648
} e-mail: mager-at-interchg.ubc.ca


From daemon Wed Oct 11 15:55:04 2000



From: jim quinn :      jquinn-at-doL1.eng.sunysb.edu
Date: Wed, 11 Oct 2000 16:47:28 -0400
Subject: Re: Recoat Phosphor Screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


GRANT SCIENTIFIC CORP
1385 ROCK ISLAND ROAD
GILBERT , SC 29054
803-892-2841 PHONE
803-892-2441 FAX

} Listers,
}
} I need to have the phosphor screens recoated on an EM 400T we have on
} campus. I have been told that Grant Scientific does a good job. I have not
} been able to find a way to contact them. Can anyone help with a phone number
} or info.? I would also welcome any other suggestions. Thanks.
}
} Joel McClintock
} EM Specialist
} Univ. of Kentucky
} Lexington, Ky
} 859-257-1242


From daemon Wed Oct 11 17:44:46 2000



From: Brian W Robertson :      brobertson-at-unl.edu
Date: Wed, 11 Oct 2000 17:38:54 -0500
Subject: EM400T available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


We are moving out our Philips EM400T TEM. It has suffered from vacuum
logic and lens logic problems -- problems that currently mean that it is
unusable. It has a STEM unit, a Quantum EDX detector, Kevex 7000 system,
and a variety of holders, including tilt-rotate. Anyone interested in some
or all of this equipment should get in touch ASAP, please.
Thanks,
Brian Robertson


***********************************************************
Assoc. Prof. Brian W. Robertson
Department of Mechanical Engineering
and Center for Materials Research and Analysis
University of Nebraska-Lincoln, N124 WSEC,
17th & Vine Sts., Lincoln, NE 68588-0656, USA
** Office 402 472 8308; FAX 472 1465; Labs 472 8762 and 472 5498 **


From daemon Wed Oct 11 20:04:17 2000



From: Vaughn Fierke :      Vfierke-at-snblusa.com
Date: Wed, 11 Oct 2000 19:56:06 -0500
Subject: Ultracut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have a Reichert Jung Ultracut S/N382797, Type701701, acquired from Upjohn
Japan. The unit has no stereoscope or power unit (lost in packaging or
shipping). Any person interested in the unit to use or for parts, please
don't hesitate to contact me.




From daemon Wed Oct 11 22:54:23 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 Oct 2000 23:54:51 -0500
Subject: Polaron CPD question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Pauline Yu wrote:
===========================================================
........................ The Polaron has been very reliable and has
served us well, but we're
worried that its age is compromising its safety.
==========================================================
Unless the unit has been dropped or otherwise abused, it is difficult for me
to see how its "age" could have anything to do with its safety. But if this
was your concern, there is a process, and we could help you arrange it, by
which the chamber is returned to the UK, and retested in the same laboratory
where it was tested originally, and essentially recertified. The testing is
done at a pressure that is far beyond where a rupture disc would blow, so
you could then use it with confidence for many years to come. The cost of
the service is far less than the cost of a new system.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================


From daemon Thu Oct 12 08:06:18 2000



From: tellis2-at-hallmark.com
Date: Thu, 12 Oct 2000 08:00:40 -0500
Subject: stub cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I experimented with several different cleaning/mounting procedures and the
one that is the most time effective for me is the following:
I apply a conductive silicone release agent to my stubs before I
apply carbon double stick tape to them, then I use a conductive Al or Cu
tape ( I cut it in half length-wise so it doesn't take up so much space )
to insure conductivity, works for my samples which are mostly dry powders,
industrial hygiene dusts, inks and papers.
Then when I want to clean the stubs I just lift the tape off with a
knife ( not to sharp ) and rinse and rub any residue off with paper towels
using a 50/50 % toluene / acetone mix, then ethanol in a ultrasonic
cleaner if I need them really clean.

Terry Ellis
Hallmark Cards Inc.
tellis2-at-hallmark.com
USA.



From daemon Thu Oct 12 08:06:18 2000



From: Zhongwen Yao :      zong-wen.yao-at-psi.ch
Date: Thu, 12 Oct 2000 08:02:18 -0500
Subject: copper eclectrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Listers, I meet some problem how to electrical-polish 3mm pure copper
disk. Would you plese tell me the good solution and the good condition
of electrical-polishing copper on tenupol-5 machine.
Thanks

Mr.Zhongwen Yao
EPFL- CRPP
Villingen, PSI
Switzerland




From daemon Thu Oct 12 08:06:18 2000



From: Gareth Robinson :      Gareth.Robinson-at-emitech.co.uk
Date: Thu, 12 Oct 2000 13:51:49 +0100
Subject: RE: Coater for use with FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Ben,

You may also want to consider the option of Chromium coating which will
give grain siz of less than 0.5NM Chromium grain size with thin film
deposition typically 5NM.

We manufacture and sell the K575 Sputter Coater unit. It has the
advantage of automated cycle coating Turbomolecular drag pumping for
High Vaccum.

Please take a look at our web site for a detailed technical brief and
the instrument specification.

HTTP://www.emitech.co.uk

Kind regards

Gareth
} -----Original Message-----
} From: Garber, Charles A. [SMTP:cgarber-at-2spi.com]
} Sent: Wednesday, October 11, 2000 7:40 PM
} To: MICROSCOPY BB
} Subject: Coater for use with FESEM
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Ben Craft wrote:
} =============================================================
} I'm getting ready to purchase a new sputter coater for our FESEM. I'd
} like to hear how lucky others were with using a "regular" sputter
} coater
} for high resolution SEM work. Currently, there is a noise problem
} with our
} SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm
} ZrO2
} close-packed particles, and 4nm pores in glass using a thin coating of
} Au/Pd
} applied with an old Polaron. One thing I'm worried about is = once the
} noise
} is fixed and I can resolve the published 1.5nm resolution will I be
} able to
} resolve the grains in the coating?
} =============================================================
} One system to consider is the Osmium Plasma Coater OPC-60 made by
} Nippon
} Laser and Electronics, Nagoya, Japan. It is distributed by SPI
} Supplies.
} You can find extensive information and examples on URL
} http://www.2spi.com/catalog/osmi-coat.html
}
} The end result is an amorphous coating so there is no grain size with
} which
} one has to contend. The source of the osmium is an ampoule of osmium
} tetroxide. This is not just a simple matter of sputtering from an
} osmium
} metal cathode. And since the coating is an inert precious group
} metal, its
} shelf life is quite long, if not infinite. Safety concerns are
} address on
} the webpage mentioned above.
}
} Contact me off-line should you want us to do a demo coating for you.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================


From daemon Thu Oct 12 08:56:21 2000



From: Gunnar Glasser :      glasser-at-mpip-mainz.mpg.de
Date: Thu, 12 Oct 2000 15:56:51 +0200
Subject: Inspection of "very thin" Au-films[PVD] by FESEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am looking for help concerning the following problem: Someone in our
institute is trying to deposit a 10nm Au-film on a Ge single crystal by
thermal evaporation from a tungsten boat (...rate: 0.5A/s).
The main point to me is, if FESEM is an appropriate method to determine if
we have really a Au-film or only gold islands ...

I do appreciate any comments concering both points (FESEM-tricks,
because 99% of our samples are uncoated insulators) and the depostition
technique/parameters for very thin gold films ...

ThanX very much in advance!

Dipl.Ing.(FH) G.Glasser
Elektronenmikroskopie
Max Planck Institut fuer Polymerforschung
Ackermannweg 10
55128 Mainz, GERMANY
phone: ++49 +6131 379195
fax : ++49 +6131 379100
web: http://www.mpip-mainz.mpg.de/~glasser

Disclaimer: The above statement is mine alone and does not implicitly represent the position of the MPI-P or the MPG!


From daemon Thu Oct 12 08:57:06 2000



From: Gunnar Glasser :      glasser-at-mpip-mainz.mpg.de
Date: Thu, 12 Oct 2000 15:58:11 +0200
Subject: Inspection of "very thin" Au-films[PVD] by FESEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am looking for help concerning the following problem: Someone in our
institute is trying to deposit a 10nm Au-film on a Ge single crystal by
thermal evaporation from a tungsten boat (...rate: 0.5A/s).
The main point to me is, if FESEM is an appropriate method to determine if
we
have really a Au-film or only gold islands ...

I do appreciate any comments concering both points (FESEM-tricks,
because 99% of our samples are uncoated insulators) and the depostition
technique/parameters for very thin gold films ...

ThanX very much in advance!



Dipl.Ing.(FH) G.Glasser
Elektronenmikroskopie
Max Planck Institut fuer Polymerforschung
Ackermannweg 10
55128 Mainz, GERMANY
phone: ++49 +6131 379195
fax : ++49 +6131 379100
web: http://www.mpip-mainz.mpg.de/~glasser

Disclaimer: The above statement is mine alone and does not implicitly represent the position of the MPI-P or the MPG!


From daemon Thu Oct 12 09:02:26 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 12 Oct 2000 09:00:05 -0500
Subject: RE: Coater for use with FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
As seen below, Gareth Robinson mentions chromium as an option
for fine coats to use in high res SEM.

A word of caution: Chromium is tricky to work with. You
cannot use chromium sputter coating the same simply way you are used
to sputtering with, say, gold or platinum. You have to keep the
atmosphere in the coater squeaky clean, and even cleaner, and you
must absolutely minimize the exposure of your coated sample to oxygen.

I expect that chromium coats can be superb, but you cannot
expect to simply put a chromium target in your sputter coater and
have decent results. We found this out the hard way.

As ever,
Tobias

}
}
}
} Ben,
}
} You may also want to consider the option of Chromium coating which will
} give grain siz of less than 0.5NM Chromium grain size with thin film
} deposition typically 5NM.
}
} We manufacture and sell the K575 Sputter Coater unit. It has the
} advantage of automated cycle coating Turbomolecular drag pumping for
} High Vaccum.
}
} Please take a look at our web site for a detailed technical brief and
} the instrument specification.
}
} HTTP://www.emitech.co.uk
}
} Kind regards
}
} Gareth Gareth.Robinson-at-emitech.co.uk

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Thu Oct 12 09:15:48 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Thu, 12 Oct 2000 10:12:38 -0400
Subject: Re: EM400T available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


At 5:38 PM -0500 10/11/00, Brian W Robertson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Brian,

I am interested in the holders.

Regards,
Lucille Giannuzzi

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Thu Oct 12 10:52:23 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Thu, 12 Oct 2000 08:50:11 -0700
Subject: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Netters

In upgrading our light microscopes, we are buying some new lenses for our
confocal scope and a brightfield scope. The salesforce is regaling us with
tales of improved resolution, clarity, brightness etc that comes with
spending double or triple the price for a standard oil immersion or dry
lens. Could someone please clarify, either on-line or off, what the
advantages and disadvantages of each of the following ( my texts on light
microscopy don't deal with them). Are they worth the extra dollars?

1) a water immersion lens for looking at cells suspended in buffer and
covered with a coverslip (water is used rather than oil between coverslip
and lens) These lenses typically have a lower numerical aperture than an
oil immersion lens.

2) a water dipping lens, used to examine cells in culture directly, without
a coverslip. I would expect the absence of refractive indice changes
(media/coverslip/air or oil) would explain their improved brightness.

Thanks in advance for your input

Steve Barlow
SDSU EM Facility




From daemon Thu Oct 12 11:18:57 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 12 Oct 2000 12:09:00 -0400
Subject: "coater" for High Resolution SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Ben:

As you are so close by to our San Clemente, CA lab, I would suggest that
you come by and bring some samples to try on our IBS/e Ion Beam Sputter
Deposition and Etching System.

The IBS/e, a thin film deposition and etching system, is designed to
improve high resolution electron microscopy imaging by depositing
ultra-thin, fine grain metal and carbon films on specimens. Even low
voltage imaging is improved.

We can deposit many different metals and carbon or show you examples of
contrast enhancement on various types of specimens from our library of
micrographs. I will send to you by separate mail some AFM images comparing
evaporated coatings, sputtered coatings and ion beam deposited films.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 8:27:56 AM on 10/11/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Ben Craft
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm getting ready to purchase a new sputter coater for our FESEM. I'd
like to hear how lucky others were with using a "regular" sputter coater
for high resolution SEM work. Currently, there is a noise problem with
our SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm
ZrO2 close-packed particles, and 4nm pores in glass using a thin coating
of Au/Pd applied with an old Polaron. One thing I'm worried about is =
once the noise is fixed and I can resolve the published 1.5nm resolution
will I be able to resolve the grains in the coating?

Thanks,
Ben {


From daemon Thu Oct 12 11:45:45 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 12 Oct 2000 11:56:53 -0500
Subject: 200 Fall Meeting of the Texas Society for Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The 2000 Fall Meeting of the Texas Society for Microscopy will be held

on October 26, 27, and 28 at the DoubleTree Hotel in Dallas, TX.
The hotel is located in Campbell Centre at 8350 N. Central
Expressway, Dallas, TX 75206. The phone number for the hotel is
800-222-TREE.
A special workshop will be held on Thursday, Oct. 26, entitled "The
SEM Today". It will cover the latest information concerning basic
SEM,
LVSEM, ESEM and detectors. Dr. David Joy, University of Tennessee,
will be
speaking on Low Voltage SEM and Variable Pressure SEM.
YOU MUST BE REGISTERED FOR THE THURSDAY
SEMINAR SERIES. It is free for members and new member applicants.
If you plan to attend and have not registered, call or email Pam Neill
at
817-568-6497 or pamela.neill-at-alconlabs.com

TSM PROGRAM
SUMMARY OF DAILY ACTIVITIES

Thursday October 26, 2000
8:30-9:00 Registration at TI
9:00-5:00 Workshop coordinated by Kevin Cronyn, Hitachi and hosted by
TI
12:30-7:00 Exhibitor setup and Poster setup DoubleTree
5:30-7:00 TSM Executive council Meeting, DoubleTree
7:00-9:00 Evening Social, DoubleTree
9:30-11:00 TSM Hospitality room DoubleTree

Friday October 27, 2000
8:00-8:30 Continental Breakfast in vendor's room
8:00-11:00 Registration
8:30-8:45 Introduction and announcements.
8:45-10:00 Platform presentations
10:00-10:15 Break in exhibitors’ room.
10:15-11:30 Guest speaker Russ Pinazotto "Electron Microscopy of
Semiconductor Nanoparticles"
11:45-1:45 Lunch and business meeting with vendors at DoubleTree Hotel
Campbell Center
1:45-3:00 Guest speaker Dr. Charles Mims “Use of High Pressure
Freezing for Biological TEM".
He will also include some information on plunge freezing.
3:00-4:30 Break in exhibitors’ room. Poster authors should be at
their posts
5:30-7:00 Social in the hospitality suite. Dinner on your own. There
will be a trip planned to
the West End via DART for dinner and entertainment.

Saturday October 28, 2000
8:00-9:00 Registration
8:00-9:00 Continental Breakfast
8:00-9:15 Poster session continued
9:30-10:45 Platform presentations
10:45 adjourn


For more information and/or to register, please contact
Pamela Neill, Program Chair TSM
Alcon Research LTD
6201 South Freeway
Forth Worth, TX 76134-2099
pamela.neill-at-alconlabs.com
817-568-6497
--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Oct 12 11:49:24 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Thu, 12 Oct 2000 12:46:52 -0400
Subject: RE: copper eclectrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Here are a few Cu electropolishing solutions:

a) 33% nitric acid, 67% methanol cooled to -25C and at 1.5 V

b) 45% phosphoric acid, 55% water at { 10C (no voltage given)

c) 10% orthophosphoric, 90% water at 0C and at 8 V.

I had success with the first solution in a Tenupol more years ago than I
care to contemplate!

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca


----------
From: Zhongwen Yao [SMTP:zong-wen.yao-at-psi.ch]
Sent: Thursday, October 12, 2000 9:02 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: copper eclectrical polishing


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.


Listers, I meet some problem how to electrical-polish 3mm pure
copper
disk. Would you plese tell me the good solution and the good
condition
of electrical-polishing copper on tenupol-5 machine.
Thanks

Mr.Zhongwen Yao
EPFL- CRPP
Villingen, PSI
Switzerland




From daemon Thu Oct 12 11:49:24 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Thu, 12 Oct 2000 12:49:05 -0400
Subject: Re: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Steve Barlow wrote:

} Netters
}
} In upgrading our light microscopes, we are buying some new lenses for our
} confocal scope and a brightfield scope. The salesforce is regaling us with
} tales of improved resolution, clarity, brightness etc that comes with
} spending double or triple the price for a standard oil immersion or dry
} lens. Could someone please clarify, either on-line or off, what the
} advantages and disadvantages of each of the following ( my texts on light
} microscopy don't deal with them). Are they worth the extra dollars?
}
} 1) a water immersion lens for looking at cells suspended in buffer and
} covered with a coverslip (water is used rather than oil between coverslip
} and lens) These lenses typically have a lower numerical aperture than an
} oil immersion lens.
}
} 2) a water dipping lens, used to examine cells in culture directly, without
} a coverslip. I would expect the absence of refractive indice changes
} (media/coverslip/air or oil) would explain their improved brightness.
}
} Thanks in advance for your input
}
} Steve Barlow
} SDSU EM Facility

So they are saying that all of their old lenses are junk?
Ask for a demo using your material at your site. Then you can see for yourself

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Oct 12 11:56:13 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 12 Oct 2000 12:03:42 -0500 (CDT)
Subject: Re: questions for Pelco CPD2 users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Responding to the message of
{Pine.GSO.4.10.10010111120030.19238-100000-at-aggie.pw.usda.gov}
from "Pauline C. Yu" {splene-at-pw.usda.gov} :
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.

} Hi listers
}
} We just bought a Pelco CPD2 with the hopes of replacing our old Polaron
} E3000, and so far, we're rather unimpressed just trying to get it through
} an initial setup.
} We were wondering if any other users could tell us how many runs they get
} out of an average CO2 cylinder? The cooling process with the CO2 seems
} somewhat inefficient at this point and we're using up a lot just trying to
} cool the chamber.

Pauline,

You should get very many runs out of an average cylinder. I'm not sure how the
Pelco is designed, but with my Ladd Company critical point dryer, also cooled by
CO2 gas flow-through, the "trick" is to set up a balance between the CO2 input
valve and the exhaust or drain valve such that you get a BIG pressure drop from
the CO2 tank pressure which is about 1000 psi - down to about 100-200 psi in the
chamber as the gas flows through. You shouldn't need to open the input valve
very much to achieve this effect. The cooling of the gas, hence the chamber,
depends on a big pressure drop, the ol' Joule-Thomposon effect, just like in a
refrigerator. When I first got my Ladd unit I had the same problem because I had
too high a pressure in the chamber during blow through of the gas.

I hope this helps. It should only take just a few minutes to cool the chamber
from room temp to about 5 C, for example, depending on size and mass of the
chamber on your unit

Good luck!

Gib.

}
} Thanks in advance,
}
} Pauline Yu
} splene-at-pw.usda.gov
} Microscopist Technician
} USDA-ARS-WRRC


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Thu Oct 12 12:02:22 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 12 Oct 2000 12:59:43 -0400
Subject: copper electrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Yao:

I have looked in our archives and have found 2 solutions that Bernie Kestel
from Argonne National Lab has developed for copper which may be of use.
This work was done with our Model 550 single vertical jet electropolisher
so references to jet height would be unique to our system. The other
parameters may need to be adjusted slightly. Please be careful in using
any of these chemicals and especially when adjusting the recipes. I owuld
suggest that you contact Bernie Kestel directly prior to using these. You
can reach him by email at kestel-at-anl.gov.

Recipe #1
90% H3PO4
10% H2O

Temp: 20 degrees C
Jet height: 6.3mm
Pump setting: 8-10
Volts: 2
Current: 70mA

Recipe #2
150 ml HNO3
350 ml methanol
40 ml butyl cellosolve

Temp: -20 degrees C
Jet height: 3.9mm
Pump setting: 2.5
Volts: 40
Current: 75mA
NOTES: excellent polish. Lower temperature result in an uneven foil
surface.

I hope this information helps. If I can be of any additional assistance,
please feel free to contact me.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 9:44:06 AM on 10/12/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Zhongwen Yao
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers, I meet some problem how to electrical-polish 3mm pure copper
disk. Would you plese tell me the good solution and the good condition
of electrical-polishing copper on tenupol-5 machine.
Thanks

Mr.Zhongwen Yao
EPFL- CRPP
Villingen, PSI
Switzerland



{


From daemon Thu Oct 12 13:02:06 2000



From: Gareth Robinson :      Gareth.Robinson-at-emitech.co.uk
Date: Thu, 12 Oct 2000 18:47:30 +0100
Subject: RE: Coater for use with FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Very true,

The K575 uses rotary and turbomolecular drag pumping to achive the high
vaccum required.

The instrument has an automated shutter assembly to allow sputter target
cleaning (Remove the oxide layer from the chromium target material)
prior to sample sputtering.

We ahve had some very good application results witht he coater, please
drop me a line for further info.

Best regards

Gareth
} -----Original Message-----
} From: Tobias Baskin [SMTP:BaskinT-at-missouri.edu]
} Sent: Thursday, October 12, 2000 3:00 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: Coater for use with FESEM
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} Greetings,
} As seen below, Gareth Robinson mentions chromium as an option
} for fine coats to use in high res SEM.
}
} A word of caution: Chromium is tricky to work with. You
} cannot use chromium sputter coating the same simply way you are used
} to sputtering with, say, gold or platinum. You have to keep the
} atmosphere in the coater squeaky clean, and even cleaner, and you
} must absolutely minimize the exposure of your coated sample to oxygen.
}
} I expect that chromium coats can be superb, but you cannot
} expect to simply put a chromium target in your sputter coater and
} have decent results. We found this out the hard way.
}
} As ever,
} Tobias
}
} }
} }
} }
} } Ben,
} }
} } You may also want to consider the option of Chromium coating which
} will
} } give grain siz of less than 0.5NM Chromium grain size with thin film
} } deposition typically 5NM.
} }
} } We manufacture and sell the K575 Sputter Coater unit. It has the
} } advantage of automated cycle coating Turbomolecular drag pumping for
} } High Vaccum.
} }
} } Please take a look at our web site for a detailed technical brief and
} } the instrument specification.
} }
} } HTTP://www.emitech.co.uk
} }
} } Kind regards
} }
} } Gareth Gareth.Robinson-at-emitech.co.uk
}
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ 109 Tucker Hall
} / / / / \ \ \ Biological
} Sciences
} /_ / __ /__ \ \ \__ University of
} Missouri
} / / / \ \ \ Columbia,
} MO USA
} / / / \ \ \ 65211-7400
} / / ___ / \ \__/ \ ____ voice:
} 573-882-0173
} fax: 573-882-0123


From daemon Thu Oct 12 13:02:06 2000



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Thu, 12 Oct 2000 13:58:30 -0400
Subject: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I have a JSM 840 that I need to relocate soon. The new location is
very near a railroad and interstate! Does anyone have information on the
optimum thickness of a concrete slab to prevent vibrations?
Thanks,

Steve Buckingham
Excellatron Solid State LLC
1460 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 770 438 2118





From daemon Thu Oct 12 13:51:35 2000



From: Bruce Brinson :      brinson-at-ece.rice.edu
Date: Thu, 12 Oct 2000 13:50:24 -0500
Subject: TEM aperture Etching oops

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi E-beam buddies,
After our last excimer laser was shut down, I had the idea of
cleaning our Pt apertures with our plasma etcher using dry air.
Optically they appear cleaner but in the TEM they are worse than before
they went into the plasma. The aperture ID edge appears to be populated
with hemispherical features that are prone to charging.
I considered back streaming from the fomblin filled mech. pump but
there is no residual smell of oil or sign of it anywhere about the
chamber. Does anyone have any idea what is going on & perhaps how these
apertures might be recovered? Some are cheap some are not.

thanks,
Bruce Brinson
Rice U.



From daemon Thu Oct 12 14:20:10 2000



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Thu, 12 Oct 2000 14:16:42 -0500
Subject: active oxygen species localization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
My goal is to localize active oxygen species/peroxidase activity in roots,
and am wondering if anyone out there has any advice. I have several
protocols, some using very different ways of utilizing DAB, and some using
cerium chloride. They range from using whole mount tissue to processing
tissue for TEM (and actually nothing in between). I am currently trying one
of the DAB protocols, but would greatly appreciate hearing from anyone who
has some insight.
Thanks again for your help,
Kristen
Kristen A. Lennon
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
kalen-at-iastate.edu



From daemon Thu Oct 12 14:59:44 2000



From: COURYHOUSE-at-aol.com
Date: Thu, 12 Oct 2000 15:55:46 EDT
Subject: Re: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Funny thing is, even some really old lenses (old brass ones)! When it comes
to low power still work pretty good!

I think many of the newer faster better sometimes what we will notice is
very slight, but sales forces tend to extol the fact we need the latest even
when perhaps we don't. i think anything produced today will only be just a
little bit if at all better than the stuff form the 80's

Now on electron microscopes things are a bunch diff. with age especially on
that old rust bucket of the edax computer We have on the AMR 1000... I
suppose I should gather some spares or just consider replacing it with a PC
when we finally get it set up and operational.

Ed Sharpe archivist for SMECC

{ { Subj: Re: light microscope lenses
Date: 10/12/00 12:21:14 PM US Mountain Standard Time
From: mcauliff-at-UMDNJ.EDU (Geoff McAuliffe)
To: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
CC: CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU, microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


Steve Barlow wrote:

} Netters
}
} In upgrading our light microscopes, we are buying some new lenses for our
} confocal scope and a brightfield scope. The salesforce is regaling us with
} tales of improved resolution, clarity, brightness etc that comes with
} spending double or triple the price for a standard oil immersion or dry
} lens. Could someone please clarify, either on-line or off, what the
} advantages and disadvantages of each of the following ( my texts on light
} microscopy don't deal with them). Are they worth the extra dollars?
}
} 1) a water immersion lens for looking at cells suspended in buffer and
} covered with a coverslip (water is used rather than oil between coverslip
} and lens) These lenses typically have a lower numerical aperture than an
} oil immersion lens.
}
} 2) a water dipping lens, used to examine cells in culture directly, without
} a coverslip. I would expect the absence of refractive indice changes
} (media/coverslip/air or oil) would explain their improved brightness.
}
} Thanks in advance for your input
}
} Steve Barlow
} SDSU EM Facility

So they are saying that all of their old lenses are junk?
Ask for a demo using your material at your site. Then you can see for
yourself

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************


} }


From daemon Thu Oct 12 15:55:14 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 12 Oct 2000 13:50:59 -0700
Subject: Re: copper eclectrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Yao,
The Struers Tenupol instructions I have recommend a solution for Cu
polishing consisting of:
250 ml phosphoric acid
500 ml distilled water
250 ml ethanol
2 ml Vogel's Sparbeize
5 g urea
"to be mixed successively" and used at 15 degrees C, 0.3 A current, 15 V,
flowrate 4 for 1 minute. I'm afraid I have no idea what "Vogel's Sparbeize" is.
Another solution I have used for the window technique is 33% (v/v) nitric
acid and 67% methanol at room temperature ( {25 degrees C), 8 V. You must
remove the sample quickly to prevent oxidation and wash in methanol. This is
a very flammable and reactive mixture used as jet fuel, so keep it away from
heat, sparks and flame.
50 ml At 08:02 AM 10/12/00 -0500, you wrote:
}
} Listers, I meet some problem how to electrical-polish 3mm pure copper
} disk. Would you plese tell me the good solution and the good condition
} of electrical-polishing copper on tenupol-5 machine.
} Thanks
}
} Mr.Zhongwen Yao

Good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Oct 12 16:03:24 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 12 Oct 2000 14:00:19 -0700
Subject: Re: Inspection of "very thin" Au-films[PVD] by FESEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gunnar,
I would recommend masking a part of the Ge by putting a bit of Al foil over
it before coating. You should be able to see the interface between the
coated and uncoated surface clearly in the FESEM due to the atomic weight
difference, and then zoom up to high magnification to see if there is any
structure in the Au film. I know in a Au sputtered film this could be seen
easily, but I haven't looked at a thermal evaporated film. It will show up
best on a polished Ge wafer. You can use higher kV (15-20) and high
resolution conditions in the FESEM, because the sample is conductive.
At 03:58 PM 10/12/00 +0200, you wrote:
} Hi,
}
} I am looking for help concerning the following problem: Someone in our
} institute is trying to deposit a 10nm Au-film on a Ge single crystal by
} thermal evaporation from a tungsten boat (...rate: 0.5A/s).
} The main point to me is, if FESEM is an appropriate method to determine if
} we
} have really a Au-film or only gold islands ...
}
} I do appreciate any comments concering both points (FESEM-tricks,
} because 99% of our samples are uncoated insulators) and the depostition
} technique/parameters for very thin gold films ...
}
} ThanX very much in advance!
}
}
}
} Dipl.Ing.(FH) G.Glasser

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Oct 12 17:40:58 2000



From: Gregory Mulhollan :      mulhollan-at-spec.com
Date: Thu, 12 Oct 2000 17:37:36 -0500
Subject: Data Translation board ouput pin connections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


MSA Listers,
This is a long shot, but if anyone of you has information on the pin output
of a DT2255 NuBus frame grabber and could forward to me off-list, I would be
very grateful. DT does not have this info on their web page and seems to be
selling support; I don't want to know how much they charge for pin output
info on obsolete boards! Thanks.

--
Gregory Mulhollan, Ph.D.
Extreme Devices Inc.
101 West 6th Street
Suite 200
Austin, TX 78701
(512)479-7740 x2231 voice
(512)479-7750 fax
mulhollan-at-extremedevices.com



From daemon Thu Oct 12 18:21:03 2000



From: Theresa Fassel :      tfassel-at-scripps.edu
Date: Thu, 12 Oct 2000 16:15:03 -0500
Subject: EM analysis of liposomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello

I would appreciate any advice/recommendations on TEM analysis of lipsomes,
particularly vesicle size.
Thanks
Theresa


Theresa A. Fassel
Core EM Unit, Mail Box MB-32
The Scripps Research Institute
10,550 N. Torrey Pines Rd.
LaJolla, CA 92037-1027
tel: (858)-784-8182
fax: (858)-784-8193
tfassel-at-scripps.edu


From daemon Thu Oct 12 23:41:14 2000



From: Ian Cotton :      icotton-at-gatan.com
Date: Thu, 12 Oct 2000 23:33:49 -0500
Subject: Job openings at Gatan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Product Manager,

Analytical Instruments

Gatan Inc. has an immediate opening for a person to handle GIF and PEELS
Product management. Gatan has a complete facility equiped with a new FEI
Tecnai 20,
a JEOL 2010/FasTEM and a Philips CM12.

The Individual should have either GIF or PEELS experience, a
strong TEM background in Biological or Materials science, good computer
skills, a willingness to travel and the vision and drive to help determine
the future of these products.

The position is based in Pleasanton, CA and
carries a salary comensurate with experience as well as a bonus plan and
Corporate success share plan.

Please contact Ian Cotton, Director of
Marketing at 925-224-7343 or email your resume to icotton-at-gatan.com.

*********************************
Ian Cotton
Marketing Director,
Gatan, Inc. 5933 Coronado Lane
Pleasanton, CA 94588 USA
Phone 925-224-7343
Fax 925-463-0204
E-mail icotton-at-gatan.com
Surf the Web to www.gatan.com
*********************************




From daemon Thu Oct 12 23:41:14 2000



From: Ian Cotton :      icotton-at-gatan.com
Date: Thu, 12 Oct 2000 23:34:24 -0500
Subject: Job openings at Gatan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Applications Manager,

Gatan Inc. has an immediate opening for a person to manage our newly equiped
demonstration facility. We have recently installed a new FEI Tecnai 20 and a
JEOL 2010/FasTEM TEM. A full suite of Gatan analytical systems ( GIF and
ENFINA PEELS ) and imaging systems are installed on these two microscopes.

The Individual should have either GIF or PEELS experience, a
strong TEM background in Biological or Materials science, good computer
skills, and excellent interpersonal skills. You should be experienced in the
management of TEM instrumentation and have good organisational skills.

This is a challenging position for an individual who wants to work with
state of the art equipment and contribute to the future development of our
products.

The position is based in Pleasanton, CA and carries a salary comensurate
with experience as well as a bonus plan and Corporate success share plan.

Please contact Ian Cotton, Director of Marketing at 925-224-7343 or email
your resume to icotton-at-gatan.com.

*********************************
Ian Cotton
Marketing Director,
Gatan, Inc. 5933 Coronado Lane
Pleasanton, CA 94588 USA
Phone 925-224-7343
Fax 925-463-0204
E-mail icotton-at-gatan.com
Surf the Web to www.gatan.com
*********************************




From daemon Fri Oct 13 02:11:07 2000



From: Ruth Yamawaki :      Ryamawaki-at-cmexchange.stanford.edu
Date: Fri, 13 Oct 2000 00:02:46 -0700
Subject: EM tissue processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


A colleague of mine is interested in purchasing a tissue processor. She has
limited access to email so please reply directly to me and let me know what
processor you use and would recommend. I'll pass the word along.

Thank you,

Ruth

***************************************
Ruth Yamawaki
Department of Comparative Medicine
Stanford University
Stanford, CA 94305
***************************************



From daemon Fri Oct 13 03:54:25 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Fri, 13 Oct 2000 10:56:41 +0000
Subject: Re: copper eclectrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


*Date sent: Thu, 12 Oct 2000 08:02:18 -0500
*To: Microscopy-at-sparc5.microscopy.com
*From: Zhongwen Yao {zong-wen.yao-at-psi.ch}
*Subject: copper eclectrical polishing

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

It seems that the best way, if you have enough money, is to buy
proper electrolyte from the Struers.
Based on our experiance, copper is difficult for TEM preparation.
We use to say that even weather has an influance on electropolishing
conditions.
Good luck,
Witold Zielinski

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75


From daemon Fri Oct 13 05:07:20 2000



From: Zhongwen Yao :      zong-wen.yao-at-psi.ch
Date: Fri, 13 Oct 2000 12:03:32 +0400
Subject: Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thank all of you who give me help very much!
Best Regards Mr.Z.Yao



From daemon Fri Oct 13 06:59:29 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 13 Oct 2000 07:52:36 -0700
Subject: Re: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Patton, David wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We slice off the specimen with a razor blade and use the
} blade to scrape the stub fairly clean. As we use colloidal
} graphite, which is conductive, I do not mind some residual
} glue as it will get covered by glue from the next use.
} This glue can be removed, if required, by sonicating in
} butyl acetate.
}
} Dave
}
} On Wed, 11 Oct 2000 21:09:07 +1000 Jim at ProSciTech
} {jim-at-proscitech.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } It all depends: how busy you are, how expensive staff time in your country is
} } and how costly the place is you buy your stubs from. Obviously some specimens
} } should be stored in a desiccator for an eternity and other can be discarded
} } immediately.
} } There is nothing magical about cleaning stubs. Solvents are messy. Best to
} } place on some newspaper a very coarse file or a sheet of coarse (50 grit)
} } sandpaper and then rub the old stub over that file of sandpaper until specimen
} } and glue are removed.
} }
} } Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this
} } leaves some money for useful purchases.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori
} } [SMTP:sojilori-at-oauife.edu.ng] wrote:
} } }
} } } Hello Guys
} } } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} } } just installed in our central science lab.
} } } Is there a way to clean stubs for reuse or are stubs disposable once used.
} } } ( This may sound naive)
} } } soji
} } } Mr. O. O. ILORI
} } } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} } } OBAFEMI AWOLOWO UNIVERSITY,
} } } ILE-IFE, OSUN STATE
} } } NIGERIA.
} } } email: sojilori-at-oauife.edu.ng
} } }
} } }
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
If you have access to a glass bead blaster, it does a great job as long
as you weren't looking for a mirror finish.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA


From daemon Fri Oct 13 07:17:21 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 13 Oct 2000 08:12:37 -0700
Subject: Re: TEM aperture Etching oops

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Bruce Brinson wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi E-beam buddies,
} After our last excimer laser was shut down, I had the idea of
} cleaning our Pt apertures with our plasma etcher using dry air.
} Optically they appear cleaner but in the TEM they are worse than before
} they went into the plasma. The aperture ID edge appears to be populated
} with hemispherical features that are prone to charging.
} I considered back streaming from the fomblin filled mech. pump but
} there is no residual smell of oil or sign of it anywhere about the
} chamber. Does anyone have any idea what is going on & perhaps how these
} apertures might be recovered? Some are cheap some are not.
}
} thanks,
} Bruce Brinson
} Rice U.
Bruce,
I've been cleaning apertures of all types for many years with 1 micron
diamond paste and a cut-knap polishing cloth, although any lint-free
cloth will work if the apertures are flat and fairly thin. Heavily
counter-sunk apertures like Siemens 2mm really benefit from the cut-knap
cloths (metallography supplies).

Finish by cleaning ultrsonically in Joy and hot water, blow dry
immediatly.

As to what is going on in your plasma system, I can't help you there.

Ken Converse
Quality Images
third party SEM service
Delta, PA


From daemon Fri Oct 13 07:42:16 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 13 Oct 2000 08:32:34 -0400
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


hi listers-


thanks for all your help in diagnosing my 35 problem. turns that the
aperture heater was the culprit. i'll make note of this for "next time"!

b-
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875


From daemon Fri Oct 13 07:52:17 2000



From: joe fu :      jofu-at-nist.gov
Date: Fri, 13 Oct 2000 08:48:56 -0400
Subject: Image analysis training course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


MSA Listers:
Is John Russ offering training course on image analysis this year? When?
Where? TIA.

Joseph Fu
National Institute of Standards & Technology
100 Bureau drive Stop 8212
Gaithersburg, MD. 20899-8212
Tel: 301-975-3495
Fax: 301-869-0822
Email: jofu-at-nist.gov




From daemon Fri Oct 13 07:53:29 2000



From: Gary Radice :      gradice-at-richmond.edu
Date: Fri, 13 Oct 2000 08:51:07 -0400
Subject: re: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I've had some experience with water immersion lenses. Their main
advantage is higher numerical aperture than dry lenses at equivalent
magnification, so better theoretical resolution. They don't offer
quite as good resolution as oil immersion lenses, but they tend to
have longer working distances, I believe, and don't have the messy
clean-up of oil immersion lenses. So, yes, they do have some distinct
advantages.

However, as others have pointed out, you may already be able to see
everything you need to see with your current lenses. And nothing
beats a test-drive.
--
Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice


From daemon Fri Oct 13 08:29:54 2000



From: DrJohnRuss-at-aol.com
Date: Fri, 13 Oct 2000 09:22:11 EDT
Subject: Re: Image analysis training course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 10/13/00 9:01:02 AM, jofu-at-nist.gov writes:

} Is John Russ offering training course on image analysis this year? When?
} Where? TIA.

Thanks for the chance to plug the courses. There will be three sessions in
2001. Dates are May 9-11 and October 30-Nov. 1, in Raleigh (at N. C. State
Univ) and June 6-8 in Denmark (at the Danish Technological Institute). Full
info on the course contents, registration info, etc., (and a downloadable
copy of the brochure for the 2000 courses, which is correct except that it
does not have the year 2001 dates) is available on-line at
http://members.aol.com/IPCourse/

It is also possible (and cost effective for groups of more than about 6-7
people) to arrange for an on-site course. Contact Cindy Allen in the NCSU
Continuing Education department at 919 515 8171 for details.

John C. Russ
John_Russ-at-NCSU.edu


From daemon Fri Oct 13 10:11:35 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Fri, 13 Oct 2000 07:49:59 -0700
Subject: Re: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Steve,

I don't think there is an "optimum" thickness for the concrete slab.
If vibration from the railroad (or any other source) is a concern most isolate
the main slab by sawing & removing the concrete under the optics console. A
new concrete slab is laid with "pea sized agregate" & felt on the sides. This
lets the column stand free from the rest of the slab.

Regards,

Earl Weltmer

"Buckingham, Steve" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi All,
} I have a JSM 840 that I need to relocate soon. The new location is
} very near a railroad and interstate! Does anyone have information on the
} optimum thickness of a concrete slab to prevent vibrations?
} Thanks,
}
} Steve Buckingham
} Excellatron Solid State LLC
} 1460 Roswell Street, Suite J
} Smyrna, GA 30080
} phone 770 438 2201
} fax 770 438 2118



From daemon Fri Oct 13 11:05:22 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 13 Oct 2000 11:01:49 -0400
Subject: RE: Cleaning Pt Apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Many years ago we regularly used platinum crucibles in many analytical
chemistry procedures. According to my analytical chemistry book, platinum
can be cleaned by fusion with potassium or sodium bisulfite. I later used
this procedure with good success to clean the platinum apertures for an RCA
EML electron microscope. Get a small ceramic crucible, put a bit of the
bisulfite in it, heat it until the bisulfite melts, then drop the apertures
into the melt. After a few minutes allow the melt to solidify, dissolve
the bisulfite with hot water, and your apertures should be as bright and
shiny as new.

If this doesnn't work, try heating them in a mixture of equal parts of
concentrated hydrofluoric acid and concentrated hydrochloric acid - using
appropriate safety precautions, of course!

Good luck

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Oct 13 11:32:33 2000



From: M. T. Coating :      mtcoating-at-meyertool.com
Date: Tue, 10 Oct 2000 09:48:04 -0400
Subject: microprobe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


To whom it may concern,
I am a materials engineering student at the Univ of Cincinnati (Ohio)
currently co-oping at a local aerospace company. They're wanting some
microprobe analysis done on some parts and have put me in charge of that.
I have a descent understanding of X-Ray diffraction but would like some
information on the specifics of a microprobe so that I know exactly what is
going on when I'm sitting watching the analysis take place. Is there any
electronic information you can send or any sites you can refer me to that
will give in depth information on the workings of a microprobe? Please
respond to keithj-at-meyertool.com.

Thank You
Keith Jones



From daemon Fri Oct 13 11:55:08 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 13 Oct 2000 12:49:21 -0400
Subject: Re: TEM aperture Etching oops

Contents Retrieved from Microscopy Listserver Archives
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Bruce,

Based on your information we believe there may be burrs on the ID edge
of your holes.
We could open up the holes a bit to provide burr-free apertures, or we
would allow you a trade in since as the only US Electron Microscope
aperture manufacturer they probably originated with us anyway.
If an aperture has a burr-free edge, a flamer should do for cleaning Pt
and if they are Moly you can clean them in an evaporator.
Even if your aperture is imported we would allow a trade in on a new
disc or strip.
Let me know if I can be of further help,

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955

Bruce Brinson wrote:
}

} Hi E-beam buddies,
} After our last excimer laser was shut down, I had the idea of
} cleaning our Pt apertures with our plasma etcher using dry air.
} Optically they appear cleaner but in the TEM they are worse than before
} they went into the plasma. The aperture ID edge appears to be populated
} with hemispherical features that are prone to charging.
} I considered back streaming from the fomblin filled mech. pump but
} there is no residual smell of oil or sign of it anywhere about the
} chamber. Does anyone have any idea what is going on & perhaps how these
} apertures might be recovered? Some are cheap some are not.
}
} thanks,
} Bruce Brinson
} Rice U.


From daemon Fri Oct 13 12:34:02 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 13 Oct 2000 12:36:37 -0500 (CDT)
Subject: Needles

Contents Retrieved from Microscopy Listserver Archives
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Mark,

Any feedback on the SEM images of the needles? Should I go ahead and finish the
other 4 that way?

At only 25X mag, seems like LM would give better images, in color, for example,
and the steel surfaces seem to show up better. The SEM images look a bit gray,
as no metal coating and low kV imaging. Metal coating would show them up better,
but I suppose that would not be allowed under the experimental constraints.

What do you think?

Gib

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Fri Oct 13 13:19:12 2000



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Fri, 13 Oct 2000 14:14:22 -0400
Subject: RE: TEM aperture Etching oops

Contents Retrieved from Microscopy Listserver Archives
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Hi Bruce,
}
} I have never tried the plasma etcher but I suspect it would remove organics
} effectively, just as a flame does. After several cleanings in the flame, we
} are usually left with deposits similar to what you describe. My old Philips
} 300 manual suggests these are usually silica-- this makes sense for our
} samples, maybe for yours as well? Same manual advises "24 hrs. immersion in
} 10% solution of HF in water". I usually save the ones I can't clean until I
} have several, then turn them over to a colleague who works with HF regularly.

} Most come back as clean as new. The hemispherical features suggest to me
that
} our contaminant will melt, but not vaporize in the flame, consistent with
} silica. You would have to look into the power of your etcher. Naturally,
} these techniques are for solid Pt apertures, and HF is not for the
} accident-prone. Good luck! By the way, our much newer Philips manual (XL30
} ESEM-FEG) gives 4 methods of heating but no mention of HF.
}
} Matt
}


Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu



From daemon Fri Oct 13 13:56:21 2000



From: Sue Barnes :      SBarnes-at-elch.org
Date: Fri, 13 Oct 2000 14:50:38 -0400
Subject: subscribe

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From daemon Fri Oct 13 14:27:59 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Fri, 13 Oct 2000 20:17:33 +0100
Subject: Fw: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
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Earl

The optimum thickness of concrete is whatever thickness of concrete it
takes to stop the train. Any other answer is incorrect.


David C

}
} ----- Original Message -----
} From: Earl Weltmer {earlw-at-pacbell.net}
} To: Buckingham, Steve {sbuckingham-at-excellatron.com}
} Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, October 13, 2000 3:49 PM
} Subject: Re: Concrete slab for SEM
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi Steve,
} }
} } I don't think there is an "optimum" thickness for the concrete slab.
} } If vibration from the railroad (or any other source) is a concern most
} isolate
} } the main slab by sawing & removing the concrete under the optics
console.
} A
} } new concrete slab is laid with "pea sized agregate" & felt on the sides.
} This
} } lets the column stand free from the rest of the slab.
} }
} } Regards,
} }
} } Earl Weltmer
} }
} } "Buckingham, Steve" wrote:
} }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } } Hi All,
} } } I have a JSM 840 that I need to relocate soon. The new
location
} is
} } } very near a railroad and interstate! Does anyone have information on
the
} } } optimum thickness of a concrete slab to prevent vibrations?
} } } Thanks,
} } }
} } } Steve Buckingham
} } } Excellatron Solid State LLC
} } } 1460 Roswell Street, Suite J
} } } Smyrna, GA 30080
} } } phone 770 438 2201
} } } fax 770 438 2118
} }
} }
} }
}



From daemon Fri Oct 13 14:40:45 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Fri, 13 Oct 2000 20:29:13 +0100
Subject: Concrete slab

Contents Retrieved from Microscopy Listserver Archives
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Earl

The optimum thickness of concrete is whatever thickness of concrete it
takes to stop the train. Any other answer is incorrect.


David C

}
} ----- Original Message -----
} From: Earl Weltmer {earlw-at-pacbell.net}
} To: Buckingham, Steve {sbuckingham-at-excellatron.com}
} Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, October 13, 2000 3:49 PM
} Subject: Re: Concrete slab for SEM
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi Steve,
} }
} } I don't think there is an "optimum" thickness for the concrete slab.
} } If vibration from the railroad (or any other source) is a concern most
} isolate
} } the main slab by sawing & removing the concrete under the optics
console.
} A
} } new concrete slab is laid with "pea sized agregate" & felt on the sides.
} This
} } lets the column stand free from the rest of the slab.
} }
} } Regards,
} }
} } Earl Weltmer
} }
} } "Buckingham, Steve" wrote:
} }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } } Hi All,
} } } I have a JSM 840 that I need to relocate soon. The new
location
} is
} } } very near a railroad and interstate! Does anyone have information on
the
} } } optimum thickness of a concrete slab to prevent vibrations?
} } } Thanks,
} } }
} } } Steve Buckingham
} } } Excellatron Solid State LLC
} } } 1460 Roswell Street, Suite J
} } } Smyrna, GA 30080
} } } phone 770 438 2201
} } } fax 770 438 2118
} }
} }
} }
}




From daemon Fri Oct 13 14:55:12 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, October 12, 2000 3:45 PM
Subject: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
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Steve,

It is not the thickness, but rather the area of the slab. It is better be
minimal. Also, the soil under the slab- rock is bad, sand is good. If you
have any choice on the matter- construction is not started yet, etc.- than
have the slab on the sand cushion, make it small, just the size of the SEM
footprint, and separate from the rest of the building.
It is possible, in some cases, to cut the existing concrete slab in order to
separate it from the rest of the structure. (Consult the construction expert
first!!) Then, fill the cuts with soft silicone, rubber, etc. in order to
prevent hard objects from getting in the cuts, as they will make an acoustic
contact, which you are trying to avoid.

Also, many options from many sources are available regarding antivibration
supports and mounts, depending on your situation and requirements. Some are
expensive, some are not. Contact me off list if you need further help.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Buckingham, Steve {sbuckingham-at-excellatron.com}
To: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}



From daemon Fri Oct 13 15:10:47 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Fri, 13 Oct 2000 13:03:39 -0700
Subject: Re: Fw: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dave,

Have you considered the speed, size, & direction of the train?
In addition, the type of concrete & it's reinforcement material (ribar) also
needs to be considered.
Also whether you want the train to transmit vibration, collide with the SEM or
land on top of the SEM needs to be calculated.

Any other answer is incorrect.

Regards,

Earl

David Cockayne wrote:

} Earl
}
} The optimum thickness of concrete is whatever thickness of concrete it
} takes to stop the train. Any other answer is incorrect.
}
} David C
}
} }
} } ----- Original Message -----
} } From: Earl Weltmer {earlw-at-pacbell.net}
} } To: Buckingham, Steve {sbuckingham-at-excellatron.com}
} } Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
} } Sent: Friday, October 13, 2000 3:49 PM
} } Subject: Re: Concrete slab for SEM
} }
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi Steve,
} } }
} } } I don't think there is an "optimum" thickness for the concrete slab.
} } } If vibration from the railroad (or any other source) is a concern most
} } isolate
} } } the main slab by sawing & removing the concrete under the optics
} console.
} } A
} } } new concrete slab is laid with "pea sized agregate" & felt on the sides.
} } This
} } } lets the column stand free from the rest of the slab.
} } }
} } } Regards,
} } }
} } } Earl Weltmer
} } }
} } } "Buckingham, Steve" wrote:
} } }
} } }
} } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } -----------------------------------------------------------------------.
} } } }
} } } } Hi All,
} } } } I have a JSM 840 that I need to relocate soon. The new
} location
} } is
} } } } very near a railroad and interstate! Does anyone have information on
} the
} } } } optimum thickness of a concrete slab to prevent vibrations?
} } } } Thanks,
} } } }
} } } } Steve Buckingham
} } } } Excellatron Solid State LLC
} } } } 1460 Roswell Street, Suite J
} } } } Smyrna, GA 30080
} } } } phone 770 438 2201
} } } } fax 770 438 2118
} } }
} } }
} } }
} }



From daemon Fri Oct 13 15:48:24 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Fri, 13 Oct 2000 13:40:37 -0700
Subject: Ortec EDS detector

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

I have an recently rebuit Ortec EDS detector with electronics (old) from
a Zeiss TEM free to anyone.

If interested, please respond offline.

Earl Weltmer
Scanservice Corp.
(714) 573-9158



From daemon Fri Oct 13 15:56:57 2000



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Fri, 13 Oct 2000 16:50:42 -0400
Subject: NYSEM Presidential Symposium 2000

Contents Retrieved from Microscopy Listserver Archives
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NYSEM Presidential Symposium 2000: "Cell Polarization: Mechanisms at the
Membrane"

The New York Society for Experimental Microscopy will hold its annual
Presidential Symposium on 19 October from 9:00 AM to 5:30 PM at Columbia
University (Hammer Health Sciences Building, Room 401*). This year's
Symposium is entitled: "Cell Polarization: Mechanisms at the Membrane".
The Symposium will focus on recent breakthroughs in understanding the
molecular mechanisms involved in the generation of cell polarity.
Symposium participants will highlight recent advances in a number of model
systems in which microscopic approaches can be combined with genetic and
molecular biological approaches to understand cell polarity. One emerging
theme that will be explored during the Symposium is the degree to which
basic molecular pathways for cell polarization have been conserved despite
the diversity of structures that result in the different systems.
Registration is free, but preregistration is required for lunch (contact
Dr. Philip Leopold, Cornell Univ. Medical School, email:
pleopold-at-mail.med.cornell.edu; phone 212-746-2255)


* The Hammer Health Sciences Building is one block west of Broadway on the
corner of Fort Washington and 168th Street. There is a subway stop at
Broadway and 168th Street.


SCHEDULE - NYSEM PRESIDENTIAL SYMPOSIUM 2000
"Cell Polarization: Mechanism at the Membrane"
19 October 2000
Columbia University
Hammer Health Sciences Building, Room 401

8:30 - 9:00 On Site Registration* and Coffee

9:00 - 9:15 Gregg Gundersen (NYSEM President): Welcome and opening remarks

9:15 - 9:55 John Cooper (Department of Cell Biology & Physiology,
Washington University, St. Louis):
"Interactions of Microtubules with the Cortex During Mitosis in Budding
Yeast"

9:55 - 10:35 Kerry Bloom (Department of Biology, University of North
Carolina - Chapel Hill
"How Microtubules Polarize Nuclear Movements in Yeast"

10:35 - 10:55 Coffee Break

10:55 - 11:35 Fred Chang (Department of Microbiology, Columbia University,
New York)
"Spatial control and cytoskeletal dynamics in fission yeast"

11:35 - 12:15 Carole Parent (National Cancer Institute, NIH, Bethesda)
"Visualizing Signaling Events in Chemotaxing Eukaryotic Cells"

12:15 - 2:00 Lunch - Riverview Lounge (adjacent to Hammer 401)

2:00 - 2:40 Gregg Gundersen (Department of Anatomy & Cell Biology, Columbia
University, New York)
"Rho GTPase Pathways Integrate the Microtubule and Actin Cytoskeletons
During Cell Migration"

2:40 - 3:20 Clare Waterman-Storer (Department of Cell Biology, Scripps
Research Institute, La Jolla)
"Microtubule and Actin Interactions in Cell Polarization and Motility"

3:20 - 3:40 Coffee Break

3:40 - 4:20 Enrique Rodriguez-Boulan (Dyson Vision Research Institute,
Cornell University, New York)
"Role of Actin Filaments and Microtubules in Polarized Delivery of Plasma
Membrane Proteins"

4:20 - 4:30 Gregg Gundersen - Closing Remarks

4:30 - 5:30 Reception - River View Lounge



****************************************************************************
Frank Macaluso tel: 718-430-3547
Analytical Imaging Facility fax: 718-430-8996
Albert Einstein College of Medicine e-mail: macaluso-at-aecom.yu.edu
1300 Morris Park Avenue http://www.aecom.yu.edu/aif/
Bronx, NY 10461
****************************************************************************



From daemon Fri Oct 13 16:44:27 2000



From: Theresa Fassel :      tfassel-at-scripps.edu
Date: Fri, 13 Oct 2000 16:03:56 -0500
Subject: TEM of dynabeads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


---------------------- Forwarded by Paula M Churt/TMG/CSC on 10/13/2000
10:59 AM ---------------------------


Diane A Ciaburri/GDDS-at-GDDS
10/13/2000 09:55 AM

To: Paula M Churt/TMG/CSC-at-CSC
cc:


Hello

Has anyone observed biological material-antibody-dynabead complex in EM?
Our user has great results with Dynabeads M-450 Epoxy Product # 140.01 in
his assay, but we would like to visualize the virus attached to the
dynabead. The manufacturer suggests the iron core may leak on sectioning
causing contamination. Any ideas?
Thanks

Theresa


Theresa A. Fassel
Core EM Unit, Mail Box MB-32
The Scripps Research Institute
10,550 N. Torrey Pines Rd.
LaJolla, CA 92037-1027
tel: (858)-784-8182
fax: (858)-784-8193
tfassel-at-scripps.edu


From daemon Fri Oct 13 18:20:05 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 14 Oct 2000 01:33:22 -0700
Subject: Re: Cleaning of SEM Mag Standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Earl

The optimum thickness of concrete is whatever thickness of concrete it
takes to stop the train. Any other answer is incorrect.


David C


----- Original Message -----
} From: Earl Weltmer {earlw-at-pacbell.net}
To: Buckingham, Steve {sbuckingham-at-excellatron.com}
Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, October 13, 2000 3:49 PM


I run in the ETCH mode with Argon. Set power level to
5%. Put in the dummy Aluminum target. Hit PROCESS
and keep the vacuum at 60mTorr-40mTorr. Try for about 30
seconds and then look at the standard. Repeat until
clean. The lower the vacuum level, the less aggressive
is the cleaning action. The plasma will strike when the
vacuum reaches 20mTorr in the ETCH mode; 40mTorr
in the PLATE mode. When done, remove the Al target
and put back in the sputter coater target.

I coat (PLATE) at 80mTorr, 5nM/minute. This gives a
nice, smooth and even coating with Au/Pd or Pt.

I'm using a Hummer VII. BTW, if you have trouble with
the plasma not striking for PLATE when vacuum reaches
40mTorr, I have a simple fix that makes it virtually
never fail to fire.

gary g.


At 07:59 AM 10/13/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
}
} 10/13/00 Subject: Cleaning of SEM Mag
} Standard
} 09:36
} AM
}
}
}
}
}
}
}
}
} Hi all,
}
} I've got an NIST 484e SEM Mag Standard that has years of carbon build-up
} ("burn marks"). I want to clean it with my plasma etcher but I'm not sure
} about the parameters to use. Does anyone have experience doing this?
}
} I have an Anatech Hummer plasma coater. Do I use the etch or plasma mode?
} Argon or air for atmosphere? What vacuum and voltage, and for how long?
}
} Thanks!
}
} Diane Ciaburri
}
} dciaburri-at-gdds.com
} (413)494-2847
} General Dynamics
} 100 Plastics Ave.
} Pittsfield Ma 01201



From daemon Sat Oct 14 11:50:20 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Sat, 14 Oct 2000 16:11:43 -0400 (EDT)
Subject: Re: TEM of dynabeads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

David is right.
Our testing has shown that saw cutting the floor to isolate the column from
the rest of the building has no affect other than to cost a lot. The
vibration is transmitted through the ground, not the bldg.

Craig Franklin
Vibration Engineering Consultants
www.vibeng.com


----- Original Message -----
} From: David Cockayne {david.cockayne-at-materials.oxford.ac.uk}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, October 13, 2000 5:16 PM


I don't know that the beads "leak", but they do not present a smooth
appearance in sections, either internally or - sometimes - at the surface.
the internal appearance can be discounted, but I always process some
control ("clean") beads in parallel. Sometimes there is a little fluffy
stuff (OK - flocculent material) on the surface of the blank beads, but it
could never (in my experience) be confused with viruses.

One other thing I would suggest is to have your researcher try to get as
much material aspossible on small beads. I once examined some IP samples
that were not very efficiently done on large beads - between the large
surface area of the beads and the low density of actual sample on the
beads, a *lot* of time was wasted just looking for the IP material. A real
aspirin project :)

I section Dynabeads on the not-so-great part of my diamond knife; I
haven't noticed new scratches from it, but I suspect that the material is
potentially dulling and scratchy.

Good luck!

Tamara Howard
CSHL


On Fri, 13 Oct 2000, Theresa Fassel wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello
}
} Has anyone observed biological material-antibody-dynabead complex in EM?
} Our user has great results with Dynabeads M-450 Epoxy Product # 140.01 in
} his assay, but we would like to visualize the virus attached to the
} dynabead. The manufacturer suggests the iron core may leak on sectioning
} causing contamination. Any ideas?
} Thanks
}
} Theresa
}
}
} Theresa A. Fassel
} Core EM Unit, Mail Box MB-32
} The Scripps Research Institute
} 10,550 N. Torrey Pines Rd.
} LaJolla, CA 92037-1027
} tel: (858)-784-8182
} fax: (858)-784-8193
} tfassel-at-scripps.edu
}
}



From daemon Sat Oct 14 18:30:44 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Sat, 14 Oct 2000 18:25:00 -0500
Subject: EM tissue processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ruth;
I would highly recommend the Ted Pella Microwave oven series 3450. I have
used this oven for over 3 years now and have processed over 500 specimens
(human and animal) without a single mishap to the tissue. In fact you may
find the results better then you would get by conventional processing. You
can contact Rick Giberson with Ted Pella's toll free number and he can give
you more specific information and a price to work with. You can contact me
at rla-at-mindspring.com as well.

Ron Austin (Research Associate)
LSU Medical Ct. at Shreveport
Dept. of Pathology
Shreveport, LA
318-675-4557




From daemon Sun Oct 15 12:15:12 2000



From: anderron-at-us.ibm.com
Date: Sun, 15 Oct 2000 13:04:43 -0400
Subject: Microscopy request from TV Show NOVA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The following is a request from a producer of the PBS TV show NOVA, which
came in to the MSA Business office.

Can anyone help? Please correspond directly to the requester.

} } } } } }

Hi Folks --

I'm a producer with the science TV series NOVA. I'm working on a show
about evolution and I'm desperately looking for a way to adapt a zeiss
axiophot microscope to an Aaton 16mm film camera. So far I've struck
out on finding anyone who has gear to accomplish this feat.

Any suggestiongs?

--
Chris Schmidt
Powderhouse Productions
259 Elm Street
Somerville MA 02144
617-629-2200 work
617-776-7905 fax
781-820-0727 mobile
617-403-8699 pager
781-646-0928 home
Chris-at-powderhouse.net





From daemon Sun Oct 15 14:07:57 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 16 Oct 2000 09:19:21 GMT+1200
Subject: Re: microprobe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. This foundation was a
significant factor in allowing us the obtain a TEM resolution better
than one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).

----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. This foundation was a
significant factor in allowing us the obtain a TEM resolution better
than one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).


----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. This foundation was a
significant factor in allowing us the obtain a TEM resolution of better
than one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).


----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. Such a foundation was a
significant factor in allowing us to obtain a TEM resolution better than
one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).



----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. Such a foundation was a
significant factor in allowing us to obtain a TEM resolution better than
one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).



----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}



} I have a descent understanding of X-Ray diffraction but would like some
} information on the specifics of a microprobe so that I know exactly what is
} going on when I'm sitting watching the analysis take place. Is there any
} electronic information you can send or any sites you can refer me to that
} will give in depth information on the workings of a microprobe?

Keith

There is a wonderful book "Handbook of Silicate Analysis", by P J
Potts, that has great chapters on many modern instrumental
techniques, including XRF and EPMA.
Although written with a geological slant, Potts combines lucidity
with solid info, and it should be interesting to you also.

cheers

rtch

ps I hope your XRD is located downstairs.


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Sun Oct 15 22:42:16 2000



From: sfield-at-island.net ()
Date: Mon, 16 Oct 2000 08:36:55 -0500
Subject: Ask-A-Microscopist: Recommendation needed booklet that will give

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Ritchie,

Thanks for the heads-up. I was working from home this morning (Sunday)
and finally noticed and stopped it. I hoped it had only gone out once
or twice, but I had received five copies this afternoon when I checked
the listserver. :-(

Cheers,
Mike

----- Original Message -----
} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}


To Mike, Craig, Earl and others,

Many years ago there was an article in JEOL News detailing the
design of floors for electron microscopes at the John Innes Institute
in the UK, and more recently I saw similarly detailed plans for a
new facility in Australia (in Brisbane, I think). We have incorporated
here aspects of the John Innes Institute plans, and do not have a
vibration problem, but as we do not have trains that run anywhere
nearby, unfortunately I have no idea whether or not these floors
would be effective in reducing the vibration being discussed!

Rob

On 15 Oct 00, at 19:03, MAOKeefe-at-lbl.gov wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} Craig, David, Earl, Steve,
}
} Increasing the mass under the microscope works. An isolated room-size
} slab of 1m thickness can reduce vertical vibration by a factor of four
} and horizontal vibration by 10 to 12 times [1]. This foundation was a
} significant factor in allowing us the obtain a TEM resolution better
} than one Angstrom [2].
}
} -Mike O'Keefe
}
} 1. "Design and implementation of a site for a one-Ångstrom TEM", John
} H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc.
} MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom
} Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and
} Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
}
} ----- Original Message -----
} } From: "Craig Franklin" {franklin-at-vibeng.com}
} Date: Saturday, October 14, 2000 8:11 am
} Subject: Re: Concrete slab for SEM
}
} } -------------------------------------------------------------------
} } ----- The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.ComOn-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html--------
} } ---------------------------------------------------------------.
} }
} }
} } Steve,
} }
} } David is right.
} } Our testing has shown that saw cutting the floor to isolate the
} } column from the rest of the building has no affect other than to
} } cost a lot. The vibration is transmitted through the ground, not the
} } bldg.
} }
} } Craig Franklin
} } Vibration Engineering Consultants
} } www.vibeng.com
} }
} }
} } ----- Original Message -----
} } } From: David Cockayne {david.cockayne-at-materials.oxford.ac.uk}
} } To: {Microscopy-at-sparc5.microscopy.com}
} } Sent: Friday, October 13, 2000 5:16 PM
} } Subject: Re: Concrete slab for SEM
} }
} }
} } } -----------------------------------------------------------------
} } -------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America} To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} ------
} } -----------------------------------------------------------------.
} } }
} } }
} } } Earl
} } }
} } } The optimum thickness of concrete is whatever thickness of
} } concrete it
} } } takes to stop the train. Any other answer is incorrect.
} } }
} } }
} } } David C
} } }
} } }
} } } ----- Original Message -----
} } } } From: Earl Weltmer {earlw-at-pacbell.net}
} } } To: Buckingham, Steve {sbuckingham-at-excellatron.com}
} } } Cc: 'Microscopy-at-MSA.Microscopy.Com'
} } {Microscopy-at-sparc5.microscopy.com} } Sent: Friday, October 13, 2000
} } 3:49 PM
} } } Subject: Re: Concrete slab for SEM
} } }
} } }
} } } } ---------------------------------------------------------------
} } ---------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com} } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } ----
} } -------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi Steve,
} } } }
} } } } I don't think there is an "optimum" thickness for the concrete
} } slab.} } If vibration from the railroad (or any other source) is a
} } concern most
} } } isolate
} } } } the main slab by sawing & removing the concrete under the optics
} } console.
} } } A
} } } } new concrete slab is laid with "pea sized agregate" & felt on
} } the sides.
} } } This
} } } } lets the column stand free from the rest of the slab.
} } } }
} } } } Regards,
} } } }
} } } } Earl Weltmer
} } } }
} } } } "Buckingham, Steve" wrote:
} } } }
} } } }
} } } -----------------------------------------------------------------
} } -------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } } } } of
} } America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } } -----------------------------------------------------------------
} } ------.
} } } } }
} } } } } Hi All,
} } } } } I have a JSM 840 that I need to relocate soon. The new
} } location
} } } is
} } } } } very near a railroad and interstate! Does anyone have
} } information on
} } the
} } } } } optimum thickness of a concrete slab to prevent vibrations?
} } } } } Thanks,
} } } } }
} } } } } Steve Buckingham
} } } } } Excellatron Solid State LLC
} } } } } 1460 Roswell Street, Suite J
} } } } } Smyrna, GA 30080
} } } } } phone 770 438 2201
} } } } } fax 770 438 2118
} } } }
} } } }
} } } }
} } }
} } }
} } }
} } }
} }
} }
} }
} }
}
}



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **


From root Mon Oct 16 02:48:57 2000
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Message-ID: {001701c03742$383d8920$c74101a3-at-materials.ox.ac.uk}


Dear Mike

I am sure that increasing the mass under the microscope works, as you say.
But the question was what is the "optimum thickness to stop vibrations".
There is only one "optimum", and I believe my answer was it.

David
----- Original Message -----
} From: {"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com}
To: Craig Franklin {franklin-at-vibeng.com}
Cc: MSA List Server {Microscopy-at-sparc5.microscopy.com} ; DavidCockayne
{david.cockayne-at-materials.oxford.ac.uk}
Sent: Sunday, October 15, 2000 8:03 PM


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. This foundation was a
significant factor in allowing us the obtain a TEM resolution better
than one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).

----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Colleagues...

Can anyone recommend a introductory text for this person. Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor
---------------------------------------------------


Below is the result of your feedback form. It was submitted by
(sfield-at-island.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday, October
15, 2000 at 23:18:56
---------------------------------------------------------------------------

Email: sfield-at-island.net
Name: Shane Field

School: Hard Knocks

State: BC

Zip: V0N 3E0

Question: I recently came into possession of an old Bushnell microscope
(the kind that is kept in some high school science labs for 2 or 3
generations). On the top of the scope it says--CENCO 506-L Bushnell Triple
Tested 667157. (This may have been the 57 Chev 283 of scopes found in
biology classrooms all over North America) Anyway, I took Physics and
Chemistry in high school and never did learn how to operate a simple
microscope. Imagine my chagrin at not being able to figure out how it works
--I am able to view objects at low power but not high power. Would you be
able to reccomend a good (but simple) Coles Notes kind of booklet that will
give me the basics of lighting,focussing/eye piece selection etc? etc.?
Thank you-TY TY TY!

---------------------------------------------------------------------------




From daemon Mon Oct 16 08:55:05 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 16 Oct 2000 09:48:33 -0400
Subject: Re: TEM of dynabeads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


At 4:03 PM -0500 10/13/00, Theresa Fassel wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Oct 16 08:56:41 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Mon, 16 Oct 2000 08:50:15 -0500
Subject: Re: Microscopy request from TV Show NOVA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
Years ago, I made 16 mm movies from a microscope as follows.
Hooked up an ordinary video camera to the scope and displayed the
image on a high quality monitor. In front of the monitor was a wooden
square box that telescoped and was black on the inside. At the end of
that was a 16 mm movie camera, focussed on the monitor. One frame of
the move was exposed in a 1/4 sec exposure so we were running much
slower than the tv raster rate. We were actually doing timelapse
studies like this and got very nice images for playback and analysis.
This was done in the lab of Ray Keller at UC Berkeley who would have
much more of the details to hand if you want to follow this route up.

Could this help??
Tobias Baskin


} The following is a request from a producer of the PBS TV show NOVA, which
} came in to the MSA Business office.
}
} Can anyone help? Please correspond directly to the requester.
}
} } } } } } }
}
} Hi Folks --
}
} I'm a producer with the science TV series NOVA. I'm working on a show
} about evolution and I'm desperately looking for a way to adapt a zeiss
} axiophot microscope to an Aaton 16mm film camera. So far I've struck
} out on finding anyone who has gear to accomplish this feat.
}
} Any suggestiongs?
}
} --
} Chris Schmidt
} Powderhouse Productions
} 259 Elm Street
} Somerville MA 02144
} 617-629-2200 work
} 617-776-7905 fax
} 781-820-0727 mobile
} 617-403-8699 pager
} 781-646-0928 home
} Chris-at-powderhouse.net

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Mon Oct 16 12:21:09 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 16 Oct 2000 11:39:59 -0500
Subject: Re: Microscopy request from TV Show NOVA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Another poster related his success at using an intermediate monitor for
getting the image onto 16 mm film. I might take that a step further.

I don't know film production techniques, but was of the impression that
much of the editing has gone digital. If that be the case, are there not
utilities to record high resolution digital movies from microscope cameras.
I thought Sony had a rather decent digital video camera that John McKenzie
was touting a year or two ago. Even better cameras should be available now.

Please remember the final use and display of the sequence. I know that film
can provide greater detail than digital for still photographs, but I doubt
that this sequence would be enlarged to the degree that resolution would be
an issue. Then there is the question of final display. If I viewed the
final result on my plain, old TV, most any camera system would provide more
resolution than I could appreciate. If it was viewed on an HDTV, more
detail would be visible, but still not as much as a good video camera
should be able to provide. Maybe an IMAX presentation would require all the
resolution a camera could provide and then some. But by then, I would guess
the resolution of the microscope would be more of a limitation than the
resolution of the camera.

In short, I would suggest collecting and processing the sequence in digital
format until the final stage and then transferring it to 16-mm if need be.

Warren Straszheim

At 01:04 PM 10/15/2000 -0400, you wrote:

} The following is a request from a producer of the PBS TV show NOVA, which
} came in to the MSA Business office.
}
} Can anyone help? Please correspond directly to the requester.
} } } } } } }
} Hi Folks --
}
} I'm a producer with the science TV series NOVA. I'm working on a show
} about evolution and I'm desperately looking for a way to adapt a zeiss
} axiophot microscope to an Aaton 16mm film camera. So far I've struck
} out on finding anyone who has gear to accomplish this feat.
}
} Any suggestiongs?
}
} --
} Chris Schmidt
} Powderhouse Productions
} 259 Elm Street
} Somerville MA 02144
} 617-629-2200 work
} 617-776-7905 fax
} 781-820-0727 mobile
} 617-403-8699 pager
} 781-646-0928 home

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking


From daemon Mon Oct 16 14:18:30 2000



From: George Langford :      amenex-at-amenex.com
Date: Mon, 16 Oct 2000 15:07:43 -0400
Subject: Rescuing outdated Polapan CT 35mm film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hallo Microscopists !

After attempting a test roll of Polapan CT 35mm instant film, I
discovered what "outdated" means in the Polaroid context - the
negative failed to strip off the celluloid and the strip looked
unusable.

Later, after finding that there are no known dealers of this
film in my area (SE PA) I retrieved the film from the waste
basket & washed it in warn water, whereupon, to my utter
amazement, usable positive images appeared out of the murk.

I am now waiting for the film strip to dry, after dipping it
in PhotoFlo as I am used to doing with Type 55 P/N negatives.

Is there a more conservative way to strip the negative off
the film ? My method seems rather crude ...

Best regards,
George Langford
amenex-at-amenex.com


From daemon Mon Oct 16 16:22:05 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 16 Oct 2000 16:13:52 -0500
Subject: Re: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


} Craig, David, Earl, Steve,
}
} Increasing the mass under the microscope works. An isolated room-size
} slab of 1m thickness can reduce vertical vibration by a factor of four
} and horizontal vibration by 10 to 12 times [1]. This foundation was a
} significant factor in allowing us the obtain a TEM resolution better
} than one Angstrom [2].
}
} -Mike O'Keefe
}
} 1. "Design and implementation of a site for a one-Ångstrom TEM", John
} H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc.
} MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom
} Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and
} Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
}
The concrete slab need to sit on dry sand to minimize coupling to the
soil/rock below it. A layer of gravel with drain tile laid in it covered
by a 2 or 3 foot layer of sand would be a starting point. If the drain
tile won't drain by gravity a sump will be needed to pump out any water. A
water barrier need to be placed below the gravel. The truly paranoid would
put down 6 inches of tamped sand, 4 inches of benotnite clay, a layer of
heavy plastic enough sand to keep the installation of gravel from
puncturing the plastic. The benonite serves as a self sealing moisture
barrier. It is a clay that swells when it gets wet. It is not particularly
expensive if you buy it from a oil field mud company. They buy it in train
car loads and sell it in truck loads or sacks.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Mon Oct 16 18:23:47 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 16 Oct 2000 16:14:21 -0700
Subject: Re: Ask-A-Microscopist: Recommendation needed booklet that will

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



} Question: I recently came into possession of an old Bushnell microscope
} (the kind that is kept in some high school science labs for 2 or 3
} generations). On the top of the scope it says--CENCO 506-L Bushnell Triple
} Tested 667157. (This may have been the 57 Chev 283 of scopes found in
} biology classrooms all over North America) Anyway, I took Physics and
} Chemistry in high school and never did learn how to operate a simple
} microscope. Imagine my chagrin at not being able to figure out how it works
} --I am able to view objects at low power but not high power. Would you be
} able to reccomend a good (but simple) Coles Notes kind of booklet that will
} give me the basics of lighting,focussing/eye piece selection etc? etc.?
} Thank you-TY TY TY!
}
Shane -

I hope that you'll be willing to go a step beyond "booklet". There's a
very good basic book; here's the review from the Project MICRO bibliography
(URL below):

Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy; it's unequalled as a
basic reference for beginners. Almost half of the book is devoted to
simple preparation methods for biological specimens and descriptions (with
good illustrations) of commonly encountered organisms. Adult. RECOMMENDED

If that's beyond your butget, shop around the MICRO bibliography. You may
find enough basic info in the website hotlink list.

Caroline

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon Oct 16 21:37:19 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Mon, 16 Oct 2000 21:30:44 -0500
Subject: EM tissue processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi George,
Well, George if you have talked to Rick and have been to a workshop I don't
know if I have any solutions for you, however, you said you were mostly
processing yeast and Drosphila Fly eyes. In my earlier day in EM I work for
a very short time on plant tissue and I found that I had to use a vacuum
device of some kind to get the resin to penetrate the tissue. Pella has a
Vacuum device you can use in the model of oven you have (which by the way is
the same as mine).
I found that nerve tissue take a little bit more fixation (3% Glut. in 0.1M
Cacodylate buffer pH 7.3) in the oven then other tissue does. The nerve I
mostly work with is sural nerve and I find that a temp setting of 37C with
an irritation time of 40sec x3. Cooling the tissue down, in wet ice, to
about 7-8C before and between each irritation period works for me.
What sort of fixative do you use with these tissues?

I have a vacuum system for that microwave here at Shreveport which is only a
3 or 4 hour trip from Dallas. Maybe you could work sometime in to come over,
bring your specimens with you.


Ron Austin (Research Associate)
LSU Medical Ct.
Dept. of Pathology
318-675-4557
Shreveport, LA
e-mail:
rla-at-mindspring.com



From daemon Tue Oct 17 02:41:21 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Tue, 17 Oct 2000 08:35:26 +0100
Subject: TEM: Request for a rubber roll membrane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Dear Electron Microscopists,

Could you please help us - please send replies to my email
address:

c.hayward-at-kingston.ac.uk


Hi,
We have a 30 year old Philips T301 transmission electron
microscope, and the rubber roll membrane (original part number
5322 360 40071) in the pneumatic film lifting mechanism of the
camera has split. In view of the age of the instrument it looks as if
we would only be able to replace it if some nice person out there
has a spare one that they might be prepared to part company with.
Is there anybody out there who has a spare camera lift roll
membrane please?

Thanks,

Bill Edwards.

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk


From daemon Tue Oct 17 04:31:46 2000



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Tue, 17 Oct 2000 10:06:25 -0400
Subject: Food Structure and Functionality Symposium 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The principle of mounting the microscope on a massive slab is
good, but I suspect that to isolate your microscope from the very low
frequency, long-wavelength vibrations caused by rail traffic you will
need a much softer and compliant isolation than can be provided by
any depth of sand or compacted aggregate. I have recently built a
garage / workshop within 50 metres of the London - Edinburgh
track. The building is on a heavy reinforced concrete raft
foundation. The slab is on 12" of sand over 3 feet of 2 to 3"
aggregate and the substrate is several metres of heavy gravel
material described by the geologists as raised beach. Nevertheless
you can feel the vibration caused by passing trains through your
feet.

My guess is that you need a fully floating slab, either mounted on
very compressible pads of rubber or similar elastomer, or raised on
an air cushion.

Vibration is a problem which many people worry about as demands
for high resolution and traffic increase, and solutions to it are
expensive. It strikes me that it would be a good move to do some
modelling of the physics of the situation before committing money to
it. Does anyone know how to go about this? Does software exist?

Chris



} From: "Gordon Couger" {gcouger-at-couger.com}
To: {R.Cross-at-ru.ac.za} , {"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com}
Copies to: {Microscopy-at-sparc5.microscopy.com}


Food Structure & Functionality Symposium 2001 -
An international symposium leading Food Structure & Functionality studies through the 21st century


Being held in conjunction with the 92nd AOCS Annual Meeting and Expo , May 13-16, 2001, Minneapolis Convention Center, Minneapolis, Minnesota, USA. For information, e-mail us at meetings-at-AOCS.org

The mandate of the Food Structure & Functionality Forum : *To promote global collaboration between Food and Agriculture professionals in Structure and Functionality disciplines by facilitating and providing a forum for exchange of knowledge, expertise and research findings*.
The symposium has two themes:
* new and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure-function relationships in foods;
* food system studies covering any part of the processing chain - from the raw material to the final product, and including trouble shooting.
preliminary program
May 13th - Short Courses (each short course will take a full day, they will be run in parallel)
1) Food Contaminants. Contact person - Mark Auty
2) Specific Labelling in Foods. Contact person - Marcel Paques

May 15th-17th inclusive - Technical sessions (6 sessions will run over 3 days)

May 14th
Morning.
Session 1. Dairy applications and fat based foods (Chairs: Mark Auty and Tony McKenna)

Afternoon
Session 2. Food Safety (Chairs: Judy Arnold and Maria Brandl )

Evening
-Round Table Discussion (RTD)
-Social program for Division members and FS&F symposium participants

May 15th
Morning
Session 3. New Methods and Techniques for Food Structure and Functionality Analysis
Chair: Kathy Groves

Afternoon
Session 4. Agricultural Applications of Microscopy and Imaging (Chairs: Delilah Wood and Paula Allan-Wojtas)

Evening
FS&F Division member meeting

May 16th
Morning
Session 5. Ingredients and Food Processing (Chairs: Diana Kittleson and Jim Charbonneau)

Afternoon
6. Colloidal and Interfacial Sciences (Chairs: Marcel Paques and David Pechak)

Poster Session
Posters will be displayed during the meeting and presented during breaks between technical sessions

Contact information for the chairs is shown below, in alphabetical order:


Paula Allan-Wojtas
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Noval Scotia Canada B4N 1J5
Tel: (902) 679-5566
FAX: (902) 679-2311
email: allanwojtasp-at-em.agr.ca

Judy Arnold
USDA -ARS - RRC
950 College Station Rd.
P.O. Box 5677
Athens, GA 30604-5677
USA
Tel: (706) 546-3515
FAX: (706) 546-3068
email: jarnold-at-ars.usda.gov

Mark Auty
Dairy Products Research Centre
TEAGASC
Moorepark, Femoy, Co. Cork
Ireland
Tel: 011-353-25-42447
FAX: 011-353-25-42340
email: mauty-at-moorepark.teagasc.ie

Maria Brandl
USDA - ARS - WWRC
800 Buchanan Street
Albany, CA 94710
USA
Tel: (510) 559-5885
FAX: (510) 559-5948
email:mbrandl-at-pw.usda.gov

James E. Charbonneau
National Food Processors Association
Food Chemistry and Packaging Department
1401 New York Ave, NW
Washington, D.C. 20005
USA
Tel: (202) 639-5972
FAX: (202) 639-5991
email: jcharbo-at-nfpa-food.org

Kathy Groves
Leatherhead Food Research Association
Randalls Road, leatherhead
Surrey KT22 7RY
England
Tel: 44 0132 822330
FAX: 44 0132 386228
email: kgroves-at-lfra.co.uk


Diana Kittleson
Pillsbury TPC Labs
737 Pelham Blvd.
St. Paul, MN 55114
USA
Tel: (651) 917-5859
FAX: (651) 917 5850
email: dkittleson-at-pillsbury.com

Tony McKenna
New Zealand Dairy Institute
Private Bag 11 029
Palmerston North,
New Zealand
Tel: 011 64 6 350 4649
FAX: 011 64 6 356 1476
email: tony.mckenna-at-nzdri.org.nz

Marcel Paques
Wangeningen Centre for Food Sciences/Unilever Research Vlaardingen
P.O. Box 20, 6710 BA Ede
The Netherlands
Tel: 011 31 318 659690
FAX: 011-31-318 650400
email: paques-at-nizo.nl

David Pechak
Kraft Technology Centre
801 Waukegan Road
Glenview, IL 60025
USA
Tel: (847) 646-4808
FAX: (847) 646-3864
email: dpechak-at-kraft.com

Delilah Wood
USDA - ARS - WWRC
800 Buchanan Street
Albany, CA 94710
USA
Tel: (510) 559-5653
FAX: (510) 559-5777
email: wood-at-pw.usda.gov


From daemon Tue Oct 17 10:32:04 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Tue, 17 Oct 2000 11:28:17 -0400
Subject: Re: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


All this talk of huge amounts of concrete and agregate and sand. . .

What about isolation platforms? While it might be slightly more
expensive - at least you
won't be left with a huge construction bill if your concrete slab
doesn't isolate enough
vibration.

TMC makes specific floor platforms. We were looking at their tables
when ordering a
Confocal Mircosope. And from the catalog and price list you can get a
platform that will
easily isolate the column from nearly all vibration for around $5000 to
$6000 dollars. It
looks like they also make active vibration cancelling systems too.

Simple things like elastomer and rubber bases for concrete slabs have a
fatigue life,
which could be shorter than the remain life of the microscope.

I have no finacial interest in TMC: http://www.techmfg.com

I cannot see how you will isolate train vibrations without a system a
little more complex
than a concrete slab.


Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)



From daemon Tue Oct 17 12:18:40 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Tue, 17 Oct 2000 11:13:23 -0600
Subject: Re: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


} } } "Buckingham, Steve" {sbuckingham-at-excellatron.com} 10/12/00 18:55 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi All,
I have a JSM 840 that I need to relocate soon. The new location is
very near a railroad and interstate! Does anyone have information on the
optimum thickness of a concrete slab to prevent vibrations?
Thanks,

Steve Buckingham
Excellatron Solid State LLC
1460 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 770 438 2118

Steve,

Have you tried something cheaper? When I was with The Johns Hopkins University School of Medicine we were remodeling the lab. A new darkroom sink for developing negatives was being put in right beside the TEM. The plumbers had to drill for water lines and a sink drain within 3-5 feet of the microscope. We lifted up each corner of the microscope and installed 60 or 80 durometer(I think it was 80), 4"x4"x1" rubber pads and could use the microscope without vibration. The lab was located just below street level. This might be a way to go before trying concrete slabs. The only way I've heard of using concrete slabs was to isolate the slab from the rest of the floor. A concrete slab could be very expensive. Most rubber supply houses can supply the pads. Hope you can work it out.

Phil Rutledge
USDA/ARS
151 Dixon Drive
Chestertown, MD 21620
voice: 410.778.4136 or 2120
fax: 410.778.4399
e-mail: rutledge-at-ars.usda.gov





From daemon Tue Oct 17 14:14:33 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Tue, 17 Oct 2000 15:13:01 -0400
Subject: MSA Videotapes available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Videotapes from M&M 2000 are now available for purchase. A new list of
all tapes in the collection can be found at the MSA web site or directly at
: http://www.biotech.ufl.edu/sems/newtape00.htm

Many of the older tapes are available for immediate shipment. Others,
including the 2000 tapes, must be ordered from the video duplication
service. This usually requires about two weeks. Please remember to order
the tapes by number and title. Make checks payable to "MSA" in US funds
only. Purchase orders can be accepted. Orders may be placed by phone,
mail, fax or e-mail to the contact at the bottom of this message.

If you are a presenter in any of the tapes and feel that your tape should
not be offered for sale in the future for whatever reason (obsolete
information, bad hair day, etc.) please let me know.

Greg Erdos
Videotape Wrangler
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251


From daemon Tue Oct 17 14:21:16 2000



From: crowj-at-mail.leon.k12.fl.us ()
Date: Tue, 17 Oct 2000 14:19:11 -0500
Subject: Ask-A-Microscopist: bacteria and fungi and yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

Can anyone answer this one?.
Remember Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
and K-12 Students looking for help so keep try to your technical details at
the right level.

Nestor
---------------------------------------------------
Below is the result of your feedback form. It was submitted by
(crowj-at-mail.leon.k12.fl.us) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, October
17, 2000 at 11:33:21
---------------------------------------------------------------------------

Email: crowj-at-mail.leon.k12.fl.us
Name: students

School: The Academic Resource Center

State: Florida

Zip: 32304

Question: Students are working with bacteria and fungi and yeast. They
want to know why yeast is killed at temperatures lower than body
temperatures, yet bacteria can grow on human bodies with a much higher
temperature (98.2 according to most sources is the average body temp).

---------------------------------------------------------------------------




From daemon Tue Oct 17 15:45:18 2000



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Tue, 17 Oct 2000 16:45:50 -0500
Subject: M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello List -
Have I waited long enough to receive my proceedings volumes from the
Philadelphia M&M meeting? If so, whom should I contact regarding
"re-shipment"?
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396


From daemon Tue Oct 17 16:28:32 2000



From: Amy McGough :      amcgough-at-bilbo.bio.purdue.edu
Date: Tue, 17 Oct 2000 16:33:17 -0500
Subject: TEM positions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Dear Colleagues,

We have both technical and postdoctoral openings for transmission
electron microscopists interested in studying biological
macromolecules and cellular assemblies to high resolution using
electron cryomicroscopy. The recent research emphasis of our
laboratory has been on the molecular mechanisms underlying the
assembly, organization, and dynamics of the cytoskeleton. Stable
funding is available for these positions for 5 years. Salaries are
negotiable.

The Department of Biological Sciences provides outstanding modern TEM
facilities including Philips CM300-FEG, CM200-FEG, and EM420 electron
cryo-microscopes dedicated to structural studies. Both the 300kV and
200kV field emission gun microscopes are outfitted with CCD cameras,
2k x 2k and 1k x 1k respectively, in addition to film cameras.

Purdue is home to over 50,000 students, faculty, and staff, and is
located in West Lafayette, Indiana - approximately 1 hour northwest
of Indianapolis and 2.25 hours southeast of Chicago. The area affords
the advantages associated with a major university, while providing an
affordable and attractive 'small town' environment in which to live.

The ideal candidates will be enthusiastic, self-motivated individuals
with strong communication skills who are committed to applying an
integrated structure-function approach to the study of biological
macromolecules. Interested individuals should contact Dr. McGough by
e-mail or phone. Formal applications for the technical positions
should be made via the Personnel Services department
http://www.adpc.purdue.edu/Personnel/job-home.htm.

Purdue is an equal access/equal opportunity university.



Amy M. McGough, Ph.D.
Assistant Professor, Department of Biological Sciences
and Editorial Board Member, FEBS Letters
Purdue University
West Lafayette, IN 47907-1392
E-mail: amcgough-at-bilbo.bio.purdue.edu
Office: 765-496-7501; Lab: 765-496-7716
Secretary: 765-494-4954; Fax: 765-496-1189
CM200F: 765-496-2746; CM300F: 765-496-7508
http://bilbo.bio.purdue.edu/~amcgough/
Biophysics & Cell Biology Symposium: http://bilbo.bio.purdue.edu/~rbernal/


From daemon Tue Oct 17 16:29:56 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 17 Oct 2000 14:27:58 -0700
Subject: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Aside from all the concrete, sand and aggregate issues, the column on our
Cameca microprobe rests on inflatable "feet" that helps reduce vibrations as
well. This of course is in addition to an isolation slab that it sits on
that was poured first.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Buckingham, Steve [mailto:sbuckingham-at-excellatron.com]
Sent: Thursday, October 12, 2000 10:59 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Hi All,
I have a JSM 840 that I need to relocate soon. The new location is
very near a railroad and interstate! Does anyone have information on the
optimum thickness of a concrete slab to prevent vibrations?
Thanks,

Steve Buckingham
Excellatron Solid State LLC
1460 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 770 438 2118





From daemon Tue Oct 17 19:24:25 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 18 Oct 2000 13:23:50 GMT+1200
Subject: Rubber Pads for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Maybe the rubber pads might help a bit, but is there actually going
to be a problem?
The 840 has really good spring suspension anyway, I can't see that
rubber pads would add significant isolation to what's there already.

Might be instructive to ask if anyone's actually experienced
vibration sensitivity with an 840.

rtch



}
}
} Hi All,
} I have a JSM 840 that I need to relocate soon. The new location is
} very near a railroad and interstate! Does anyone have information on
} the optimum thickness of a concrete slab to prevent vibrations?
} Thanks,
}
} Steve Buckingham
} Excellatron Solid State LLC
} 1460 Roswell Street, Suite J
} Smyrna, GA 30080
} phone 770 438 2201
} fax 770 438 2118
}
} Steve,
}
} Have you tried something cheaper? When I was with The Johns Hopkins
} University School of Medicine we were remodeling the lab. A new
} darkroom sink for developing negatives was being put in right beside
} the TEM. The plumbers had to drill for water lines and a sink drain
} within 3-5 feet of the microscope. We lifted up each corner of the
} microscope and installed 60 or 80 durometer(I think it was 80),
} 4"x4"x1" rubber pads and could use the microscope without vibration.
} The lab was located just below street

Phil Rutledge USDA/ARS 151
} Dixon Drive Chestertown, MD 21620 voice: 410.778.4136 or 2120 fax:
} 410.778.4399 e-mail: rutledge-at-ars.usda.gov
}
}
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Tue Oct 17 19:25:45 2000



From: Jennifer E. Taylor :      jtaylor-at-stevens-tech.edu
Date: Tue, 17 Oct 2000 20:23:05 -0400
Subject: PEG sample prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear List Members,
}
} We are trying to section by microtome samples of solution-cast PEG.
PEG
} is dissolved in water and THF and allowed to rest until it becomes a
} viscous solution by the evaporation of the solvent. It is then put
into
} molds and under vacuum to further rid the material of solvent.
Finally,
} the PEG becomes solid.
}
} THE PROBLEM: The PEG moldings can not be sectioned because they are
too
} brittle and crumble in the chuck.
}
} Does anyone have a protocol for preparing PEG samples that does not
lead
} to this end?
}
} Thank you very much,
}
} Jennifer Taylor



From daemon Wed Oct 18 03:47:59 2000



From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Wed, 18 Oct 2000 09:42:05 BST
Subject: Re: Ask-A-Microscopist: bacteria and fungi and yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear students

Thanks for your interest. There are around 500-600 different species
of yeast, many of them unrelated genetically. Some of these, such as
some species of Sporobolomyces have temperature requirements that are
below normal body heat (37C). Others, such as Candida albicans, are
pathogenic on humans, with their optimum temperature at normal body
heat. The bakers' yeast, Saccharomyces cerevisiae, can survive 40C
for short periods, but has its growth optimum at around 27C. There
are specially selected strains (especially used in brewing) which
have other growth optima.

Whilst I do not know the details, I believe that bacteria have
similarly varied optimal growth temperatures (e.g. the iron bacterium
Gallionella at 20C, the yoghurt bacterium Streptococcus thermophilus at
over 45C).
I hope this helps.

Cheers

Stephan Helfer PhD
Mycologist, Royal Botanic Garden Edinburgh

} Email: crowj-at-mail.leon.k12.fl.us
} Name: students
}
} School: The Academic Resource Center
}
} State: Florida
}
} Zip: 32304
}
} Question: Students are working with bacteria and fungi and yeast. They
} want to know why yeast is killed at temperatures lower than body
} temperatures, yet bacteria can grow on human bodies with a much higher
} temperature (98.2 according to most sources is the average body temp).
}
} ---------------------------------------------------------------------------
}
}
}
}

Cheers

Stephan
0131 248 2865
FAX 248 2901
http://www.rbge.org.uk


From daemon Wed Oct 18 04:05:25 2000



From: Jim.Slater-at-mcmail.vanderbilt.edu
Date: Tue, 17 Oct 2000 18:53:27 -0500
Subject: H-600

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The Department of Cell Biology -at- Vanderbilt University has a Hitachi H-600
TEM which we would like to disassemble and remove.

If interested in obtaining this equipment item contact Jim Slater by
October 26, 2000 -at- jim.slater-at-mcmail.vanderbilt.edu or (615) 343-4905.

Jim Slater
Administrative Officer
Department of Cell Biology
Vanderbilt University School of Medicine
Nashville, TN 37232-2175





From daemon Wed Oct 18 06:55:45 2000



From: Larry Allard :      L2A-at-ornl.gov
Date: Wed, 18 Oct 2000 07:53:05 -0400
Subject: Re: M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
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Robert:

you may know this already, but...

I called on Monday regarding the missing Proceedings, and found that
there were 3 mailing lists, and they are now working on the third
list, so it could be an additional 2 weeks before the final volumes
are received. Hang in there...

Larry





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Oak Ridge National Laboratory
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865-574-4981
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allardlfjr-at-ornl.gov


From daemon Wed Oct 18 07:54:10 2000



From: Joseph Neilly :      joe.p.neilly-at-abbott.com
Date: Wed, 18 Oct 2000 07:52:48 -0500
Subject: PEG sample prep.

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Jennifer,

PEG comes in different molecular weights. You might try a lower molecular
weight PEG whcih would be less crumbly.

Also, John Wolosewick developed a technique to section biological tissue
embedded in PEG. When I worked in his lab, we used to attach the PEG
embedded tissue to aluminum stubs with dental wax and put the stub in the
chuck of the microtome. That would prevent the chuck of the microtome from
crushing your sample.

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202
voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com






jtaylor-at-stevens-tech.edu on 10/17/2000 09:21:39 PM
Please respond to jtaylor-at-stevens-tech.edu
To: Microscopy-at-sparc5.Microscopy.Com
cc:


Dear List Members,
}
} We are trying to section by microtome samples of solution-cast PEG.
PEG
} is dissolved in water and THF and allowed to rest until it becomes a
} viscous solution by the evaporation of the solvent. It is then put
into
} molds and under vacuum to further rid the material of solvent.
Finally,
} the PEG becomes solid.
}
} THE PROBLEM: The PEG moldings can not be sectioned because they are
too
} brittle and crumble in the chuck.
}
} Does anyone have a protocol for preparing PEG samples that does not
lead
} to this end?
}
} Thank you very much,
}
} Jennifer Taylor







From daemon Wed Oct 18 08:20:08 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 18 Oct 2000 09:17:12 -0400
Subject: Re: PEG sample prep.

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At 8:23 PM -0400 10/17/00, Jennifer E. Taylor wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

The problem may be the molcular weight of PEG that you are using. Years
ago, I used PEG to embed heart muscle for TEM, and if memory serves, we
used a mixture of MW 10,000 and 6,000 to obtain a "sectionable"
consistancy. Our source of information at that time was work by John
Wolosowick (this was in the early-to-mid 1980's). A similar mixture may
work for you. I'lm sorry I can't be more speciic, but that was work done
at another institution, for a PI who has since passed away, so I don't have
immediate access to the records.
Try looking up John's work (It was Wolosowick & Keith Porter, I believe).

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Wed Oct 18 08:48:47 2000



From: Jennifer Jackson :      JJackson-at-nu.ac.za
Date: Wed, 18 Oct 2000 08:45:45 -0500
Subject: BSA-gold uptake

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Hi

I'm planning to BSA-gold uptake into human cell-culture for TEM. Cell lines
are grown in DMEM-F12. With the aim to study the ultrastructure of the
endocytic system and determine the pH of the various organelles. Many
methods state that they grew the cells overnight in serum-free media before
starting the uptake. Does anybody know if this alters the morphology and/or
pH of the cell?

Jennifer Jackson
University of Natal, Pietermaritzburg
South Africa
email: jacksonj-at-nu.ac.za.




From daemon Wed Oct 18 09:59:50 2000



From: Ford M. Royer :      froyer-at-bitstream.net
Date: Wed, 18 Oct 2000 09:50:04 -0500
Subject: Ultra Microtome Available

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I have the following ultramicrotome available for sale.

Reichert (Leica) "Supernova" WITH Vibration Table ...$3,500.00

Excellent condition. Sold "As-Is, Where-Is, in Working Condition".

Please contact me directly if you are interested.

Thanks,

~ Ford
--
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
phone: 800-565-1895, Ext. 17
fax: 612-929-1895
Email: froyer-at-bitstream.net
web site: http://www.aibltd.com




From daemon Wed Oct 18 10:04:59 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Wed, 18 Oct 2000 10:00:24 -0500
Subject: Re: PEG sample prep.

Contents Retrieved from Microscopy Listserver Archives
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When I did some PEG work, a piece of the PEG embedded sample was glued to a
blank epoxy block and then sectioned. There are several papers out there on
the technique.

Polyethylene Glycol (PEG) Embedding and Subsequent De-embedding as a Method
for the Structural and Immunocytochemical Examination of Biological
Specimens by Electron Microscopy. Histake Kondo. 1984. J. Electr. Micros.
Tech., 1:227-241.

The application of polyethylene glycol (PEG) to electron microscopy. J.J.
Wolosewick. 1980. J. Cell Biol., 86:675-681.

Greg

"Jennifer E. Taylor" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear List Members,
} }
} } We are trying to section by microtome samples of solution-cast PEG.
} PEG
} } is dissolved in water and THF and allowed to rest until it becomes a
} } viscous solution by the evaporation of the solvent. It is then put
} into
} } molds and under vacuum to further rid the material of solvent.
} Finally,
} } the PEG becomes solid.
} }
} } THE PROBLEM: The PEG moldings can not be sectioned because they are
} too
} } brittle and crumble in the chuck.
} }
} } Does anyone have a protocol for preparing PEG samples that does not
} lead
} } to this end?
} }
} } Thank you very much,
} }
} } Jennifer Taylor

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Wed Oct 18 10:15:19 2000



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Wed, 18 Oct 2000 11:11:08 -0400 (EDT)
Subject: digital info replies

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Hi Listers,

On Oct 10 I posted a request for digital imaging info on behalf of graphics
prof Jonathan Lipkin (jlipkin-at-ramapo.edu). I've seen a reply from Warren
Straszheim (thanks, Warren!) but I've discovered that in that time period I
didn't receive an unknown quantity of incoming email (including my own
posting) and have no idea if anyone else responded also. So if any of you
posted a reply to Jonathan and assumed I'd been able to forward it to him,
I'd appreciate it if you'd send it to me again privately.

Many thanks,
Dee


***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Wed Oct 18 10:49:16 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Wed, 18 Oct 2000 11:44:51 -0400
Subject: available print processors

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I have 3 print processors looking for homes - 2 free, one (new) for some
fee.

Check out details at MSA's surplus equipment site:
http://www.msa.microscopy.com/SurplusEquipment/SurplusListings.html


Ann Hein Lehman
EM Facility Manager
Trinity College
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman


From daemon Wed Oct 18 12:39:54 2000



From: Edwards, Mark S (NINDS) :      yosh-at-codon.nih.gov
Date: Wed, 18 Oct 2000 13:35:05 -0500
Subject: JEOL 200CX specimen holder

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Some time back Gatan made a ±60+ single hole, non-rotary specimen holder
for the JEOL 200-CX. They are no longer in stock and I was hoping to
find one, or the equivalent, gathering dust somewhere that I could buy
and put to good use. Reply privately to {treese-at-marinebio.mbl.edu} . I
would also be interested in any surplus single tilt holders for the JEOL
that I could try to modify for ±60 tilt. Thanks!....Tom Reese "



From daemon Wed Oct 18 13:17:30 2000



From: Billfester-at-aol.com
Date: Wed, 18 Oct 2000 14:14:50 EDT
Subject: Re: light microscope lenses

Content