My amateur results with a microtome have been less than satisfactory. Embedding in paraffin has been tire some and not very successful. Sectioning raw or fix material has only worked on a very limited range of things.
I picked up an old A0 Microtome and a new knife that I believed used CO2 as a coolant. I can get CO2 but it is a hassle and I have to build a stage and delivery system for it since the stage is missing.
I am planning on building a simpler stage using copper on an insulating base and use propane or 1,1,1,2,tetrafloroethane. The tests I have done with tetrafloroethane look very promising. It reaches -62 f and is very easy to use. It is normally used to chill parts on circuit boards when looking for problems. It comes in a spray can with a plastic tube.
Modifying a propane torch is also an easy solution. I have worked with propane all my life and I am well aware of the dangers. Having to go outside to use it is one of the main reasons for using the tetrafloroethane.
My questions are: Will spraying the sample directly with the coolant cause more damage to the sample than freezing indirectly through the stage? If so can I just use a larger sample and trim away the damaged area? Are there any better readily available coolants?
Thanks Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
Perhaps the membranes are leaching out during the dehydration and infiltration. Try a rapid dehydration, such as, two minutes each in 50, 70, 95, and one change of 100% EtOH. Then infiltrate in 3:1 EtOH:Epon for ten minutes, 1:1 for 10 minutes, 1:3 for twenty minutes, full strength Epon for thirty minutes, change to fresh full strength Epon and polymerize. Glutaraldehyde fix for only one hour, maximum, and that is longer than actually needed for cell monolayers. Stick with your en bloc staining method prior to dehydration. Also, perhaps thicker sections, like 70 nm, would increase your contrast. Good luck!
Dotty Sorenson Microscopy and Image Analysis Laboratory Department of Cell and Developmental Biology University of Michigan Medical School Ann Arbor, Michigan (734)763-1170 dsoren-at-umich.edu
On Tue, 31 Oct 2000, Xinran Liu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We currently doing some EM work on rat cultured hippocampal cells. } The purpose of this experiment is to visualize individual synaptic vesicles } by transmission electron microscopy. The problem we had is that the } resolution/contrast is not high enough to distinguish the synaptic vesicles. } } The following is what we did, cells were cultured on coverslips, they were } fixed in situ in 2% glutaraldehyde for 1 hr to 24 hrs, postfixed with 1% } OsO4 for 1 hour, and en bloc stained with 2% aqueous uranyl acetate for 30 } min. dehydrated in ethanol and embedded in Polybed812. 40-50 nm sections } were stained by 5% uranyl acetate and lead citrate prior to EM observation. } } I would appreciate it very much if someone would provide suggestions on } section staining or cell processing. } } Xinran } } ******************************************** } Xinran Liu, M.D., Ph.D. } Center for Basic Neuroscience } The University of Texas Southwestern Medical Center at Dallas } 6000 Harry Hines Blvd., NA4.214A } Dallas, TX 75390-9111 } Phone: (214) 648-1830 } Fax: (214) 648-1801 } E-mail: xinran.liu-at-utsouthwestern.edu } }
} We currently doing some EM work on rat cultured hippocampal cells. } The purpose of this experiment is to visualize individual synaptic vesicles } by transmission electron microscopy. The problem we had is that the } resolution/contrast is not high enough to distinguish the synaptic vesicles. } } The following is what we did, cells were cultured on coverslips, they were } fixed in situ in 2% glutaraldehyde for 1 hr to 24 hrs, postfixed with 1% } OsO4 for 1 hour, and en bloc stained with 2% aqueous uranyl acetate for 30 } min. dehydrated in ethanol and embedded in Polybed812. 40-50 nm sections } were stained by 5% uranyl acetate and lead citrate prior to EM observation. } } I would appreciate it very much if someone would provide suggestions on } section staining or cell processing. } } Xinran } } ******************************************** } Xinran Liu, M.D., Ph.D. } Center for Basic Neuroscience } The University of Texas Southwestern Medical Center at Dallas } 6000 Harry Hines Blvd., NA4.214A } Dallas, TX 75390-9111 } Phone: (214) 648-1830 } Fax: (214) 648-1801 } E-mail: xinran.liu-at-utsouthwestern.edu
Sounds like your sections are too thin for adequate (for your needs) contrast. Thinner sections are not automatically "better". Try cutting pale gold sections and see if that helps. ALSO, check the alignment and stigmation of your microscope. You may need a different size condernser aperature and/or a different size objective aperature. All of these can affect contrast and resolution.
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
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Dear Cameron,
Thank you very much for your reply (I had a week off and found it just now) - it was the only reply I have received.
The letter was written and signed by our Senior Technician and when we sent it, it bounced back, obviously because his name is not on the list. Therefore we added in the second posting that the responses should be forwarded to my address - I did not intend to deprive the microscopy community of the replies and am quite happy to post this again for all to see - especially your helpful reply.
Thank you very much - if that saves our aging TEM it would make me very happy.
Regards Claudia
} From: Cameron Hind {Cameron_Hind-at-baxter.com}
EMPLOYMENT OPPORTUNITY
HKL Technology, a global leader in electron diffraction systems for scanning electron microscopes, announces an opening in its North American headquarters in Burnt Hills, New York. Burnt Hills is located in Saratoga County, in New York’s Capital District, centrally located about 3 hours from New York City, Boston, and Montreal. HKL Technology is a small, high technology firm growing in a competitive market. The company is currently seeking an individual with advanced skills and experience in materials characterization, which must include scanning electron microscopy and electron or x-ray diffraction, to take responsibility for our applications lab. A masters or doctoral degree in physics, materials science, or geological science is required. Candidates should have positive experience in teaching others, as training new and existing users is a vital role for this position. A successful candidate will be gregarious and confident, and must enjoy traveling. If you are interested in this position, send e-mail to sutliff-at-hkltechnology.com providing your full name, current address, and the contact information of two references. Resumes should be sent by regular mail to the address below.
John A. Sutliff HKL Technology, Inc. P.O. Box 179 (801 Saratoga Road) Burnt Hills, NY 12027 sutliff-at-hkltechnology.com www.hkltechnology.com TEL:(518)384-0101 FAX:(518)384-0281
Anyone know about Semper & Sprynt Boards and can help? (Send replies to Richard Hunton {Hunton-at-Cardiff.ac.uk} , not me!)
Hi Folks:
I'm fairly new to this business so I hope that you'll forgive what might seem to be a 'dumb' question.
I am currently using a LEO / Cambridge S360 SEM with a 4QBSD. My problem is that when I use probe currents of greater than about 18nA I get what looks like a reflection of the four diode quadrants of the detector superimposed on the backscattered image.
This appears (pretty much) independant of the working distance and magnification. The SE image *does* have a dark spot on the picture where the centre of the 'reflected' quadrant ghost appears.
I *would* like to be able operate at much higher probe currents than this if possible. I am using 20 kV and I even changed the filament (you always hope, don't you?).
Any help would be most gratefully recieved.
Robert
Robert McDonald SEM & EPMA Laboratories Department of Geography - Division of Earth Sciences Gregory Building Lilybank Gardens Glasgow University Glasgow G12 8QQ Scotland
Tel 0141 330 5505 (SEM lab) or 0141 330 5442 (Microprobe Lab) fax 0141 330 4817
Specimen Preparation Engineer Northwestern University
The Electron Probe Instrumentation Center (EPIC) at Northwestern University has an immediate opening for a specimen preparation expert. EPIC is a part of the world renowned Materials Research Center (MRC) and the Department of Materials Science & Engineering at Northwestern. The EPIC facility serves over 120 users in all aspects of Scanning and Transmission Electron Microscopy. The role of the specimen preparation engineer is to assist users with their specimen preparation needs, including instruction in TEM and SEM sample preparation using IBT, FIB, PIPS, electropolishing, ultramicrotomy, cutting/grinding/polishing, vacuum evaporation etc. All microscopes in EPIC are under full service contract. Thus, the duties include training students/users, development of specialized techniques and applications, minor maintenance, record keeping and billing. A BS or technical degree in physical/biological sciences is required. The candidate must have hands-on experience in all aspects of specimen preparation as well as considerable familiarity with digital acquisition, processing and computer assisted techniques. All levels of experience will be considered. Compensation will be commensurate with experience and qualifications. Send cover letter, resume and three references to: Prof. Vinayak P. Dravid, Director EPIC Materials Science & Engineering Northwestern University, 3013A MLSB Evanston, IL 60208 E-mail: v-dravid-at-northwestern.edu Fax: (847) 491-7820 http://epic.ms.nwu.edu/epic/index.htm
Northwestern University is an Affirmative Action/Equal Opportunity Employer. Hiring is contingent upon eligibility to work in the United States.
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It is possible to use an osmium-potassium ferrocyanide fix to enhance membranes (see Karnovsky, 1971, Russell and Burguet, 1977). However, the downside is potential bleaching of ribosomes. I would be interested in hearing from others as to their experience with this type of tissue preparation. Debby
Debby Sherman Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: sherman-at-btny.purdue.edu 1057 Whistler Building West Lafayette, IN 47907-1057
On Wednesday, November 1, 2000, Geoff McAuliffe {mcauliff-at-UMDNJ.EDU} wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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Hi all, I need to find someone to work on a RMC 6000-XL ultramicrotome. The machine has lots of internal vibration on the up/retracted stroke but it is stable in the cutting stroke...it will cut a section then shake the dookey out of it so that it is impossible to form a ribbon. The machine is on an anti-vibration table. any help would be greatly appreciated. thanks much, Beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
Hello Xinran, I en bloc stain my cell cultures in 2% UA in 70% EtOH. I leave them in the 4 degree refrigerator overnight, at least 16 hours. The rest of my processing sounds comparable to yours. This seems to give good contrast. I haven't worked with hippocampal cell cultures, though, so don't now how it would work on them. Good luck, Jo Dee
Xinran Liu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } We currently doing some EM work on rat cultured hippocampal cells. } The purpose of this experiment is to visualize individual synaptic vesicles } by transmission electron microscopy. The problem we had is that the } resolution/contrast is not high enough to distinguish the synaptic vesicles. } } The following is what we did, cells were cultured on coverslips, they were } fixed in situ in 2% glutaraldehyde for 1 hr to 24 hrs, postfixed with 1% } OsO4 for 1 hour, and en bloc stained with 2% aqueous uranyl acetate for 30 } min. dehydrated in ethanol and embedded in Polybed812. 40-50 nm sections } were stained by 5% uranyl acetate and lead citrate prior to EM observation. } } I would appreciate it very much if someone would provide suggestions on } section staining or cell processing. } } Xinran } } ******************************************** } Xinran Liu, M.D., Ph.D. } Center for Basic Neuroscience } The University of Texas Southwestern Medical Center at Dallas } 6000 Harry Hines Blvd., NA4.214A } Dallas, TX 75390-9111 } Phone: (214) 648-1830 } Fax: (214) 648-1801 } E-mail: xinran.liu-at-utsouthwestern.edu
-- Jo Dee Fish Coordinator of Electron Microscopy Cell Analysis Facility The Burnham Institute 10901 N. Torrey Pines Rd. La Jolla, CA 92037 (858)646-3100 ext. 3620
I routinely prepare hippocampal neurons grown on glass coverslips for thin section EM using microwave fixation. I fix for 16 seconds (8 on-20 off-8 on) on ice with chilled fixative (2% glutaraldehyde in 0.1M cacodylate buffer). For secondary fixation, I fix 16 seconds on ice with chilled 1-2% osmium tetroxide in 0.1M cacodylate buffer containing 0.8% potassium ferricyanide. I let this sit for 5 mins before proceeding with 30 seconds of 5% en bloc uranyl acetate. Let the dish sit for 30 mins. Proceed with microwave dehydration (10 seconds each ethanol change, 3 times 100%) and infiltration (5 mins each). I cut 70 nm thin sections (pale gold/silver) and stain with 5% aqueous UA for 15-30 mins. followed by 3 mins. with lead citrate (made fresh each time). In regards to Xinran Liu's problem, there could be several factors. The fixation time is too long- 20 minutes of bench fixation is adequate. Are you putting sections on formvar? This could reduce contrast. Also, make thicker sections (60-70nm). Thirdly, check your uranyl acetate. I was using old UA that had expired and was useless. A new bottle made enormous difference. Freshly made stains help a lot, particularly for cell cultures that have low contrast. You should be able to see synaptic vesicles no problem. If these things don't help, the problem could be with the health of your cultures. Synaptic vesicles are the first to go in sick cultures. So make sure the cultures are healthy and save yourself a lot of time and effort! Good luck.
JoAnn Buchanan Molecular and Cellular Physiology Stanford University School of Medicine Stanford, CA 94305 650-723-5856
Dookey? I haven't come across that one yet up here in the Great White North. Just in case you missed it, RMC announced not too long ago that they had 're-emerged' from the hiatus caused by Ventana getting out of the EM products business. They are alive and well in Tucson and could likely point you in the direction of someone in your area. Contact info:
Al Coritz Sales & Service Manager RMC-Boeckeler Instruments 4650 S. Butterfield Dr. Tucson, AZ 85714 Voice: 520-745-0001 Cell: 520-465-3598 Fax: 520-745-0004 Email:Al-at-Boeckeler.com Website:RMCProducts.com
Hope you find someone suitable, eh? (That's Canuck-talk).
Tom Malis Group Leader - Characterization Materials Technology Laboratory Natural Resources Canada (Govt. of Canada) 568 Booth St., Ottawa, Canada ph. 613-992-2310 FAX 613-992-8735 email: malis-at-nrcan.gc.ca
} ---------- } From: } beth-at-dogwood.botany.uga.edu[SMTP:beth-at-dogwood.botany.uga.edu] } Sent: Thursday, November 02, 2000 10:01 AM } To: microscopy-at-sparc5.microscopy.com } Subject: RMC microtome service } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi all, } I need to find someone to work on a RMC 6000-XL ultramicrotome. The } machine } has lots of internal vibration on the up/retracted stroke but it is stable } in the cutting stroke...it will cut a section then shake the dookey out of } it so that it is impossible to form a ribbon. The machine is on an } anti-vibration table. } any help would be greatly appreciated. } thanks much, } Beth } } ************************************** } Beth Richardson } EM Lab Coordinator } Botany Department } University of Georgia } Athens, GA 30602 } } Phone - (706) 542-1790 } FAX - (706) 542-1805 } Email - beth-at-dogwood.botany.uga.edu } } "Between the two evils, } I always pick the one I never tried before". Mae West (1893-1980) } ************************************** } } }
{html} {font face="Arial, Helvetica"} POSITION OPEN # 1 of 2 {br} {br} Postdoctoral Scholar/Research Associate {br} {br} Cryo-Electron Microscopy of Soft and Hybrid Nanostructures {br} {br} Northwestern University {br} Materials Science & Engineering, Evanston, IL {br} {br} A postdoctoral scholar/research associate position is immediately available at Northwestern University in the area of advanced Analytical Cryo-TEM of soft and hybrid nanostructured materials. {br} This project is being supervised by Prof. Vinayak P. Dravid, and concerns with analysis of nanoscale structure, assembly/organization and chemistry of advanced soft and hybrid nanostructures (such as patterned DNA/Lipids, nanoparticle-DNA assemblies and nanoparticle-Lipid complexes etc..) using Analytical TEM and Cryo-EM techniques. This is a part of extensive cross-disciplinary and collaborative activities in nanotechnology at Northwestern. As a result, the candidate would have ample opportunity to learn diverse aspects of nanotechnology, from soft nanolithography to dynamic measurements, while contributing to Cryo-TEM analysis of soft and hybrid nanostructures. {br} Northwestern’s electron probe instrumentation center (EPIC: {a href="http://epic.ms.nwu.edu/" eudora="autourl"} http://epic.ms.nwu.edu {/a} ) is well equipped with several modern SEMs, TEMs and extensive specimen preparation equipment ranging from focused ion beam (FIB) to cryo-transfer with LN2 and LHe stages. One of the cold FEG TEMs (Hitachi HF-2000) will soon be equipped with a Gatan Imaging Filter (GIF) which will be extensively utilized in 2-D mapping of element- and bonding specific spectral signatures in soft and hybrid nanostructures. {br} The position requires a PhD in physical/biological sciences/engineering. Considerable hands-on experience in cryo-preparation techniques (e.g. freeze fracture/drying, ultramicrotomy) for soft/hybrid specimen is required. Experience in analytical and cryo-TEM techniques and computation/simulations is necessary. Prior knowledge of imaging filter/spectral imaging is desirable but not mandatory. {br} The position is available immediately for at least two years with possibility for extension for additional years upon mutual agreement. {br} Salary and compensation would commensurate with experience, in the range: $ 30,000-$40,000 per year. {br} Please forward resume with three names of references to: {br} Prof. Vinayak P. Dravid {br} Materials Science & Engineering {br} Director, Electron Probe Instrumentation Center (EPIC) {br} 2225 N. Campus Drive, 1133 MLSF {br} Northwestern University, Evanston, IL 60208, USA {br} Ph.: (847) 467-1363, Fax: (847) 491-7820 {br} E-mail: v-dravid-at-northwestern.edu {br} URL: {/font} {a href="http://vpd.ms.nwu.edu/" eudora="autourl"} {font face="Arial, Helvetica" color="#0000FF"} {u} http://vpd.ms.nwu.edu {br} {br} {br} {/a} {/font} {/u} {font face="Arial, Helvetica"} POSITION OPEN # 2 of 2 {br} {br} Postdoctoral Scholar/Research Associate {br} {br} Analytical TEM and Electron Holography of Nanostructured Materials {br} Northwestern University, Evanston, IL, {u} USA {br} {br} {/u} A postdoctoral scholar/research associate position is immediately available at Northwestern University in the area of advanced Analytical TEM of nanostructured materials. {br} This project is being supervised by Prof. Vinayak P. Dravid, and concerns with analysis of nanoscale chemistry, structure and electrostatic fields at interfaces and junctions in advanced nanostructures (nanocrystals, nanotubes, thin film interfaces) using analytical TEM and holography techniques. This is a part of extensive cross-disciplinary and collaborative activities in nanotechnology at Northwestern. As a result, the candidate would have ample opportunity to learn diverse aspects of nanotechnology, from soft nanolithography to dynamic property measurements while contributing to TEM analysis of nanostructures. {br} Northwestern’s electron probe instrumentation center (EPIC: {a href="http://epic.ms.nwu.edu/" eudora="autourl"} http://epic.ms.nwu.edu {/a} ) is well equipped with several modern SEMs, TEMs and extensive specimen preparation equipment ranging including a focused ion beam (FIB) system. One of the cold FEG TEMs (Hitachi HF-2000) will soon be equipped with a Gatan Imaging Filter (GIF) which will be utilized in 2-D mapping of element- and bonding specific spectral signatures in nanostructures. {br} The position requires a PhD in physical sciences/engineering. Considerable hands-on experience in advanced TEM techniques such as HRTEM, EDS/EELS, CBED and computation/simulations is required. Experience in electron holography is desirable but not mandatory. {br} The position is available immediately for two years with possibility for extension upon mutual agreement. {br} Salary and compensation would commensurate with experience, in the range: $ 30,000-$40,000 per year. {br} Please forward resume with three names of references to: {br} Prof. Vinayak P. Dravid {br} Materials Science & Engineering {br} Director, Electron Probe Instrumentation Center (EPIC) {br} 2225 N. Campus Drive, 1133 MLSF {br} Northwestern University, Evanston, IL 60208, USA {br} Ph.: (847) 467-1363, Fax: (847) 491-7820 {br} E-mail: v-dravid-at-northwestern.edu {br} URL: {/font} {a href="http://vpd.ms.nwu.edu/" eudora="autourl"} {font face="Arial, Helvetica" color="#0000FF"} {u} http://vpd.ms.nwu.edu {br} {br} {br} {br} {/a} {/font} {/u} {br} {div} ******************************************************* {/div} {div} (Vinayak P. Dravid) {/div} {div} Materials Science & Engineering {/div} {div} Director, Electron Probe Instrumentation Center (EPIC) {/div} {div} 2225 N. Campus Drive, 1133 MLSF {/div} {div} Northwestern University, Evanston, IL 60208, USA {/div} {div} Ph.: (847) 467-1363, Fax: (847) 491-7820 {/div} {div} E-mail: v-dravid-at-northwestern.edu {/div} {br} {div} {a href="http://nuinfo.nwu.edu/materials/faculty/vpd.html" EUDORA=AUTOURL} http://nuinfo.nwu.edu/materials/faculty/vpd.html {/a} {/div} {div} or {/div} {div} {a href="http://vpd.ms.nwu.edu/" EUDORA=AUTOURL} http://vpd.ms.nwu.edu {/a} {/div} ******************************************************* {/html}
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We are in the preliminary throes of writing a grant proposal for a new SEM. This scope will have the following characteristics: EDX, cathodoluminescence, beam etching, BS, "conventional" SEM use, possibly variable pressure. Questions 1. What are the advantages/disadvantages of Schottky over cold FE. 2. I have personally dealt with conventional filament replacement (W), I gather that FE emitter replacement is much more involved than W filament replacement. What are practicalities of FE (thermal or cold) emitter replacement with respect to who performs the work and the cost of the emitter. 3. Is there any reason that variable pressure will preclude the use of the scope for beam etching. Thanks to all Bruce Bruce Cutler Director, Microscopy & Electronic Imaging Lab University of Kansas
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id OAA01294 for dist-Microscopy; Thu, 2 Nov 2000 14:30:06 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id OAA01284 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Thu, 2 Nov 2000 14:29:35 -0600 (CST) Received: from mail (madison.tec.wi.us [198.150.15.232]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id OAA01274 for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 2 Nov 2000 14:29:08 -0600 (CST) Message-Id: {200011022029.OAA01274-at-ultra5.microscopy.com} Received: from stu.madison.tec.wi.us (nimsstu1.madison.tec.wi.us [198.150.15.195]) by mail; Thu, 02 Nov 2000 14:27:37 -0600 Received: from dflatoff [198.150.245.87] by stu.madison.tec.wi.us with Novell Internet Messaging System Web Client; Thu, 02 Nov 2000 14:27:39
Greetings All, I would like to request guidance on protocols for preparing TEM cross section to view the interface between diamond like carbon (DLC) coating on a steel substrate which has been preimplanted to increase the adhesion of the coating. Any assistance with be greatly appreciated.
Greetings All, I would like to request guidance on protocols for preparing TEM cross section to view the interface between diamond like carbon (DLC) coating on a steel substrate which has been preimplanted to increase the adhesion of the coating. Any assistance with be greatly appreciated.
In the early to mid-90s, our library, due to budget constraints at the time, let our subscriptions to microscopy journals lapse. We are now in the position to resubscibe to a number of journals. Could I get advice on which journals microscopists feel are the most useful. This includes journals in both the biolgical and physical sciences, applied research electron microscopy, as well as techniques oriented periodicals. Thanks for your input.
Mark J. Grimson Electron Microscopy Facility Dept. of Biology, MS 3131 Texas Tech University Lubbock, Texas 79409
MICROSCOPE INTERFACE DESIGNER - Public Imaging Facility Fixed-term Position
The Exploratorium is a museum dedicated to the public understanding of science, art, and human perception. Founded in 1969 by Frank Oppenheimer, it has pioneered the role of museums as active teaching centers with original programming based on an interactive approach to learning. It serves as an interdisciplinary resource for schools, universities, scientists, and artists, as well as for the public.
SUMMARY The Microscope Interface Designer will provide technical expertise to a three-year project to develop a microscopic imaging facility for the public, including museum visitors, students, teachers and Internet users, to view a variety of living specimens via video and the Internet. The facility will utilize the highest quality optics and state-of-the-art microscopic techniques and provide a unique experience for the general public to interact with the technology and tools used by biomedical researchers. The project will require extensive inter-organizational and external collaborations with research facilities, equipment and software companies, teachers and curriculum developers, and media technology developers. The Microscope Interface Designer will work collaboratively with the Exploratorium's Life Sciences staff and report to the Life Sciences Area Manager.
ESSENTIAL FUNCTIONS · Design and develop approaches for presentation of living specimens to the public in an accessible and comprehensible manner · Work collaboratively with the Visitor Research & Evaluation Department to incorporate visitor feedback in the development process · Design and develop hardware and software interface systems to enable public to easily use laboratory grade microscopes · Work closely with technical experts at equipment and software companies to develop design and technical options that enhance the project's success · Design and oversee fabrication of all necessary technical hardware for visitor interfaces with microscopes · Work collaboratively with software developers to implement software designs · Maintain and operate microscopes during the development phase
MINIMUM QUALIFICATIONS · MS degree in biological sciences with a focus in microscopy, Ph.D. desirable · Minimum 3 years experience with microscopic imaging techniques · Demonstrated experience in working with biological specimens of various sorts, particularly living tissue preparations · Familiarity with a variety of tissue culturing techniques · Familiarity with device control and software interfaces for computers · Proven ability to solve technical design problems · Ability to work collaboratively as well as independently
HOW TO APPLY: This is a three-year, fixed-term, exempt, union position which pays $788.63/week. This position starts as soon as possible and ends on or before September 30, 2003. Please apply to: Dept. LS-3L Exploratorium, 3601 Lyon Street, San Francisco, CA 94123 Fax: (415) 561-0370 E-Mail: resume-at-exploratorium.edu (attachments not accepted) No phone calls, please The Exploratorium is committed to a diverse workforce
See our web site at www.exploratorium.edu
Kellin Defiel Human Resources Exploratorium San Francisco, CA 94123 kellind-at-exploratorium.edu (415) 561-0337
Does anyone out there have a 40 kV/LaB6 EHT power supply box (aka Wallace unit) for a Cambridge 250 Mark 2 that they're willing to donate, sell, or lend for diagnostics? Or even just the mains inverter board? My users are stacked in a holding pattern and we'd all be grateful for some help!
Many thanks, Dee
*************************************************************** Dee Breger Mgr. SEM/EDX Facility Lamont-Doherty Earth Observatory 61 Route 9W Palisades, NY 10964 USA T: 914/365-8640 F: 914/365-8155
http://www.ldeo.columbia.edu/micro http://www.discovery.com/area/science/micro/micro1.html http://www.lsc.org/antarctica/front.html Journeys in Microspace (Columbia University Press, 1995)
Can anyone answer this question . Remember Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS looking for help so keep try to your technical details at the right level.
Nestor
================================================================ Email: adam.boyes-at-virgin.net Name: Adam Boyes School: Horndean Sixth Form
Question: When looking at plant cells containing chloroplasts through a microscope, why is it that they move around the edges of the cell clockwise and counterclockwise in adjacent cells?
What kind of steel? If you have a heat treated hard steel such as 440C or M50, you are in for a lot of trouble. When you thin these down, the internal stresses can bend the sample like crazy. You are best off doing a cross section using the slotted-D approach. If your sample is magnetic, an added benefit is that it minimizes the amount of magnetic material in the sample. Slot a rod that fits into a 3mm OD tube. I used 304SS tubing and rods from Small Parts Catalog. You must thin the sample to where they don't curl. Cut them down to fit in the slot and epoxy them in. Cut your samples so that the blade cuts perpendicular to the normal of the layers, i.e. along the direction of the slot. You should be able to dimple and ion mill normally. By normally, I mean low angle. The carbon resists ion milling. You might need to use a gas mixture. We had a little paper in the MRS Sample Prep IV, Vol 480, that showed a comparison of Ar, Ne, Ne/O2 or Ar/O2 to help with the carbon. Another option is to put water vapor into the ion mill while you are milling with a leak valve.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
} -----Original Message----- } From: Daniel L Flatoff [mailto:dflatoff-at-stu.madison.tec.wi.us] } Sent: Thursday, November 02, 2000 3:28 PM } To: Microscopy-at-sparc5.microscopy.com; Microscopy-at-sparc5.microscopy.com } Subject: TEM prep of DLC coating on steel } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html } } } -------------------------------------------------------------- } ---------. } } } Greetings All, } I would like to request guidance on protocols for } preparing TEM cross section to view the interface between } diamond like carbon (DLC) coating on a steel substrate } which has been preimplanted to increase the adhesion of the } coating. Any assistance with be greatly appreciated. } } } } Daniel L. Flatoff } } }
haven't received a response from my last message so I'll send it again: does anyone know who supports and supplies parts for the Technics Micro Ion Mill? (ours was made 1976)
cheers Liz McKenzie
******************************************************* Geomicrobiology and Electron Microscopy Laboratory Room S9 Cramer Hall 1721 SW Broadway Portland State University Portland OR97201
It is possible that your car engine is driven by psycho-kinetic energy. But if it looks like a petrol engine and smells like a petrol engine, a sensible working hypothesis is that it is a petrol engine.
I would appreciate any procedures or references concerning preparation of solder joints for microelectronic applications. The solders that I am concerned with are high Pb, Pb-Sn-Ag and Au-Sn. Thanks.
The advantages/disadvantages of cold vs. Schottky FE guns lie at the extremes of performance. Cold FE will give you better ultimate image resolution (roughly 1.5 vs. 2.5 nm, depending on manufacturer, of course) and will give you noticably better images at low voltage (1kV or so). Schottky will give you much higher currents at larger spot sizes (for x-ray mapping or back-scatter diffraction pattern acquisition, for example) and much better medium-term (tens of minutes to a few hours) beam current stability, if that is important to you. It is also not necessary to flash the tip periodically in a Schottky gun. For most other SEM needs, either form will work wonderfully for you.
We have one of each, and we do not notice any difference in the maintenance needs between them. Don't worry about tip replacements. Our cold FE is nearly 6 years old and is still running with the original tip, as is our Schottky, which is over three years old. I don't think this is a function of vendor - it is my impression that microscopes from other vendors have similar records. The tip replacement is performed by the service engineers, and depending on your service contract, may be included in that. It takes about 3 days (I am told!) on either instrument.
It is much more important to be clear about your microscopy needs. You say in your posting "possibly variable pressure" ... there is a big difference between the ESEM of FEI/Philips and the variable pressure designs of other manufacturers, so you need to be clear about how important this factor is to you. What structures will you need to examine by "conventional" SEM, how important is high-speed x-ray mapping, etc. etc. etc. By asking these kinds of questions the right microscope will eventually become clear to you. Don't worry about the mechanics of the gun - they all work fine.
All in my humble opinion, of course!!
Tony Garratt-Reed.
At 02:19 PM 11/02/2000 -0600, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
** Anthony J. Garratt-Reed ** MIT Room # 13-1027 ** 77 Massachusetts Avenue ** Cambridge, MA 02139-4307 ** USA ** ** Phone: (+) 1-617-253-4622 ** Fax: (+) 1-617-258-6478 **
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{Subject: Re: Freeze-plunge device availability {From: Dennis C. Winkler, DCWinkler-at-aol.com
{Last month you posted a query about the availability of freeze-plunge devices.
{I am interested in the responses you received. I didn't find them {posted on the listserver or on your web site. Would you mind sharing {them?
I have not had a chance to post them yet but here is the information. I received one response and I knew of one other vendor.
1) BAL-TEC Plunge Freezing System TFD 010 Contact Johnny Hagen at 603-622-5011 for the details. They have a single picture on their website www.bal-tec.com List price for the system is $8674
2) This summer at MSA I saw that Gatan had a system. Our sales person is Paul Miller, 724-779-2501 Their website is www.gatan.com but I saw no information on this system there the last time I looked. List price ~$20,000 (ball park figure)
The two systems do quite different things. The Bal-tec system is designed to keep the cryogens at the proper temperature and it has a pneumatic cylinder plunging arm. The Gatan system has an enclosed chamber around the sample to maintain humidity and you program in how long you want to do wicking before you plunge. You can do single sided or double sided wicking and it looks like it has a fair amount of flexibility for doing different types of plunge experiments.
As usual... the disclaimer is that I am not receiving kickbacks from either company on this.
Norm Olson
******************************************* Norm Olson Senior Research Electron Microscopist Department of Biological Sciences Lilly Hall of Life Sciences Purdue University West Lafayette, IN 47907
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id JAA01667 for dist-Microscopy; Fri, 3 Nov 2000 09:09:03 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id JAA01663 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 3 Nov 2000 09:08:33 -0600 (CST) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id JAA01656 for {microscopy-at-sparc5.microscopy.com} ; Fri, 3 Nov 2000 09:08:03 -0600 (CST) Received: from northrelay02.pok.ibm.com (northrelay02.pok.ibm.com [9.117.200.22]) by e4.ny.us.ibm.com (8.9.3/8.9.3) with ESMTP id KAA203496; Fri, 3 Nov 2000 10:06:38 -0500 Received: from d01ml065.pok.ibm.com (d01ml065.pok.ibm.com [9.117.250.65]) by northrelay02.pok.ibm.com (8.8.8m3/NCO v4.95) with ESMTP id KAA24862; Fri, 3 Nov 2000 10:06:15 -0500 Importance: Normal
We tripod polish with some modification to the procedure: You can't use water to polish the specimen, we use propylene glycol from a squeeze bottle when actually polishing the solder; The thin solder section is mechanically weak, we mount on a Mo grid with a small aperture size prior to second side polishing; Use low-angle ion milling to keep the solder from melting and to minimize element-selective milling in the ion mill if you have to ion mill the specimen. Use a cold stage in the TEM or restrict the beam heating.
Good luck,
Ron
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
IBM Analytical Services; http://www.chips.ibm.com/services/asg
} } Hello all - } } I've written to the list in the past regarding embedding of bovine } oocytes and I wish to express thanks to all of the helpful suggestions that } I received. } } I'm still having a coupld of problems, though, so I'm again seeking } advice. I am fixing oocytes in gluteraldehyde and then embedding them in } agarose. I am using 1% low melting point agarose, creating a bubble into } which I place my oocytes and then sealing the bubble with 0.5% agarose. I } then trim the agarose into a 1 mm^2 chip and dehydrate through a series of } ethanol steps, including staining with eosin to help visualize the eggs. } Finally, I clear in xylenes and embed the chip in a paraffin block. } } My problem is twofold: 1) following sectioning and rehydration, } when I } look at the agarose sections, I can certainly see oocytes (they stain } specifically for an anti-ZP antibody)and they're consistent in placement } among consequent sections, but frankly they're horribly ugly. The zona is } "wrinkled", and the eggs look like they've undergone an osmotic trauma. 2) } The agarose sections seem to frequently fold or rip and I'm not quite sure } at what stage of the process that this is occuring - I feel when placing } the sections into the waterbath or retrieving them on slides (treated with } HistoPrep) they seem fine. Is it possible the folding is occuring during } the deparaffination/rehydration, maybe because the agarose sections don't } stick well to the slides? } } Any help in this matter is greatly appreciated! Thanks! } } --Carrie
Carrie Golash John O. Almquist Research Center Penn State University University Park, PA 16802 W: (814) 865-5896 H: (814) 692-7926 http://www.das.psu.edu/dbrc/dbrc.htm
Folks, heres a new thread.... We are curious about how well automated ICC/IHC machines work How easy are they to use? How flexible are the programs? How much time do they save? Can you program sophisticated multicolor protocols? (of course the inflammatory question) which is the best machine? Whats the reagent usage like? etc
Looking forward to hearing from you all Simon
--------------------------- Simon C. Watkins Ph.D. MRC Path Associate Professor, Cell Biology and Physiology Director: Center for Biologic Imaging BSTS 225 University of Pittsburgh Pittsburgh PA 15261 Tel:412-648-3051 Fax:412-648-8330 http://sbic6.sbic.pitt.edu -----------------------------------
In the past, cold FE sources gave the best resolution, however, Schottky (thermal) FE sources have improved significantly over the years. Since the "resolution" specifications provided by the SEM vendors cannot always be compared directly, it is best to use your own samples and observe the imaging capabilities of each SEM for yourself. Such demos are common before the sale of FE SEMs.
A difference between cold FE and Schottky FE sources that is not subjective is the stability of the beam current. Cold FE sources will typically have frequent spikes and/or jumps of the beam current on the order of +/-3% or more. Readings of the beam current taken just seconds apart may show changes on this order, and over 1 hour intervals changes of +/-10% or more are not unusual. In contrast, the FE sources are exceptionally stable with total noise and drift of less than 1%/hour. (I've measured ~1% drift over 12 hours in one case.)
If you are concerned about beam current noise and drift, it may be helpful to request a graph of beam current vs. time showing the typical performance of the SEM in question. (But be wary of chart recording traces that may have a slow time constant which will hide fast changes in the beam current!)
Note that for imaging, such noise/drift in the beam is not generally an issue, because the cold FE SEMs dynamically adjust the brightness displayed on the imaging screen to compensate for the changes in the actual beam current. In effect, the beam intensity on the specimen is still changing, but the displayed image does not show it.
If you need high beam currents, the Schottky source will typically give much more current than a cold FE source.
Regarding replacement of the FE source, that is generally done under the SEM service contract. The price I have heard for the FE source itself is ~$20k US, although I'm sure others can give more exact numbers. The SEM vendors themselves will be the best source of information regarding pricing.
Variable pressure SEMs will typically have a "high vacuum" mode for conventional viewing. However, a variable pressure SEM may have an extra aperture to support the differential pumping in the column that may limit the lowest magnification, even in non-VP mode.
I hope this helps.
Joe _________________________________________ Joe Nabity, Ph.D. JC Nabity Lithography Systems E-Beam Lithography using Commercial SEMs & STEMs PO Box 5354, Bozeman, MT 59717 USA Voice: (406) 587-0848 FAX: (406) 586-9514 E-mail: info-at-jcnabity.com Web: www.jcnabity.com
In a message dated 11/2/2000 7:04:37 PM Mountain Standard Time, bcutler-at-eureka.idl.ukans.edu writes:
} We are in the preliminary throes of writing a grant proposal for a new SEM. } This } scope will have the following characteristics: EDX, cathodoluminescence, } beam } etching, BS, "conventional" SEM use, possibly variable pressure. } Questions } 1. What are the advantages/disadvantages of Schottky over cold FE. } 2. I have personally dealt with conventional filament replacement (W), I } gather } that FE emitter replacement is much more involved than W filament } replacement. } What are practicalities of FE (thermal or cold) emitter replacement with } respect } to who performs the work and the cost of the emitter. } 3. Is there any reason that variable pressure will preclude the use of the } scope } for beam etching. } Thanks to all } Bruce } Bruce Cutler } Director, Microscopy & Electronic Imaging Lab } University of Kansas }
I was involved in preparation, and microscopy, of lead solders for some time at my last job, and if you wish to contact me I will be able to put you in touch with the group that I was working with.
Hello I would like to subscribe to your service, please let me know if there is any service charge. Please pass on my problem to your subscribers. I am a research lab that does a lot of hard tissue processing, I as well as our EM departments have been using SPURR as our plastic of choice. I have been recently told by my supplier (Marivac) that in six months one of the components of SPURR will be discontinued. The component is ERL (Vinylcyclohexene Dioxide). I would like to know if others are using SPURR and if so have they encountered the same problem. Please forward your information to mmendes-at-mtsinai.on.ca. Thank-you in advance for any help offered.
Greetings. I am a final student of the department of Electrical Electronics, Obafemi Awolowo University, Ile-Ife, Nigeria. I am doing a project titled "Low-Frequency Surface Microscopy". My supervisor wants me to use an electric field-probe to detect changes in the capacitance of the surface as cracks, boundaries, etc. are encountered. I would be very grateful if you can send me any information on the topic.
My e-mail address is: femi_adeluyi-at-yahoo.com and my address is: Olufemi Adeluyi Department of Electronic and Electrical Engineering Obafemi Awolowo University, Ile-Ife, Nigeria.
Dear all, We have been asked to visualize the manufacturers stamped numbers on a rifle which have been removed by grinding. So far secondary and backscatter images don't tell us much. We are told that the metal under the stamping is compressed for a significant depth. Any ideas on how to image this compressed metal. Cheers, Eric Hines Microscopy Centre CSIRO Entomology Canberra OZ
you can use the macro etching techniques and macro photography; more details in the book Vander Voort - Metallography, Principles and practise, McGraw-Hill
best regards
KJ Huebner
{hubner-at-IOd.krakow.pl} :-)
On Mon, 6 Nov 2000, Eric Hines wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear all, } We have been asked to visualize the manufacturers stamped numbers on a } rifle which have been removed by grinding. So far secondary and backscatter } images don't tell us much. } We are told that the metal under the stamping is compressed for a } significant depth. Any ideas on how to image this compressed metal. } Cheers, } Eric Hines } Microscopy Centre } CSIRO Entomology } Canberra OZ } } } }
As promised, the only reference for SEM visualization of damaged serial numbers in gun metal I was able to quickly find was "Visualization of a Restored Serial Number Using Scanning Electron Microscopy (SEM)" by Amy Mongan of Forensic Analytical Specialties. The article was published as a case report in the Journal of Forensic Sciences [J Forensic Sci 1996;41(6):1074-1076]. There are more than likely other references but I do not have them immediately available.
Good luck!
S. Frank Platek, M.S. Forensic Chemistry Center U.S. Food and Drug Administration 6751 Steger Drive Cincinnati, OH 45237-3097 (513) 679-2700 X254 (513) 679-2761 FAX fplatek-at-ora.fda.gov
Disclaimer: Opinions/recommendations stated are solely mine and do not necessarily represent those of the US Food and Drug Administration or any other Federal Agency.
There is a special etch that "highlights" the stampings, forget SEM methods. I do not recall the exact mix, but Police forensics lab know it. I believe it is a mild form of aqua reiga with one added item but forgot. I consulted to a forensics lab and did it once and we got a few digits off an engine block.
Good luck
Robert Sherman Applied Surface Technologies www.co2clean.com
} } Dear all, } We have been asked to visualize the manufacturers stamped numbers on a } rifle which have been removed by grinding. So far secondary and backscatter } images don't tell us much. } We are told that the metal under the stamping is compressed for a } significant depth. Any ideas on how to image this compressed metal. } Cheers, } Eric Hines } Microscopy Centre } CSIRO Entomology } Canberra OZ
Some forensic scientists have been successful in detecting stamped serial numbers by etching the part in a solution of 5 -10% HNO3 in water or alcohol for 10 to20 seconds. The disturbed metal will etch more darkly than the surrounding metal.
Best regards and good luck,
Sam Purdy Tecnical Center National Steel Corp. Trenton MI spurdy-at-nationalsteel.com
The immuno units available are becoming many and varied. However, the main consideration is flexibility of the unit and secondarily cost of the reagents. Dako and Cytlologix are the most reliable and flexible available for routine and special procedures. I believe both can be programmed for dual staining or to separate protocols can be used on tissue for the result. These units allow you to choose your reagents, antibodies and procedures. The Ventana is an excellent unit with one huge drawback you are forced to use their secondaries and limited on primaries to some extent. The cost is very high for the reagents to run the unit. I am a histologist who also does EM and can speak from experience of working with the units in the field as an observer and user. Pam Marcum
-----Original Message----- } From: simon watkins [mailto:swatkins+-at-pitt.edu] Sent: Friday, November 03, 2000 11:35 AM To: microscopy
Folks, heres a new thread.... We are curious about how well automated ICC/IHC machines work How easy are they to use? How flexible are the programs? How much time do they save? Can you program sophisticated multicolor protocols? (of course the inflammatory question) which is the best machine? Whats the reagent usage like? etc
Looking forward to hearing from you all Simon
--------------------------- Simon C. Watkins Ph.D. MRC Path Associate Professor, Cell Biology and Physiology Director: Center for Biologic Imaging BSTS 225 University of Pittsburgh Pittsburgh PA 15261 Tel:412-648-3051 Fax:412-648-8330 http://sbic6.sbic.pitt.edu -----------------------------------
Maria, We are not discontinuing Spurr's at Polysciences. We do have ERL and the other components available now and in the future.
Pamela A Marcum Histology/Microscopy Product Development Manager Polysciences, Inc. 400 Valley Road Warrington, PA 19876 Phone: 800-523-2575 Ext 167 Fax: 215-343-0214 E-Mail: pmarcum-at-polysciences.com {mailto:pmarcum-at-polysciences.com}
-----Original Message----- } From: Mendes, Maria [mailto:mmendes-at-mtsinai.on.ca] Sent: Sunday, November 05, 2000 8:34 AM To: Microscopy-at-sparc5.microscopy.com
Hello I would like to subscribe to your service, please let me know if there is any service charge. Please pass on my problem to your subscribers. I am a research lab that does a lot of hard tissue processing, I as well as our EM departments have been using SPURR as our plastic of choice. I have been recently told by my supplier (Marivac) that in six months one of the components of SPURR will be discontinued. The component is ERL (Vinylcyclohexene Dioxide). I would like to know if others are using SPURR and if so have they encountered the same problem. Please forward your information to mmendes-at-mtsinai.on.ca. Thank-you in advance for any help offered.
It has been many years since I worked in a Crime Lab, but the technique that we used to raise serial numbers on guns did not involve the SEM at all. First, we polished the surface where the serial number had been ground off using successively finer grades of sandpaper. When we had achieved a highly polished appearance to the surface we then brushed the area with a dilute solution of nitric acid using a cotton tipped swab. This was done very carefully, inspecting the surface after each wipe with the swab using a collimated light beam held at a shallow angle with respect to the surface. The numbers will appear due to the fact, as you mentioned, they are compressed more than the surrounding metal and therefore are etched at a different rate. When the numbers are visible, the surface is flushed with water and a non-volatile liquid like glycerine is applied to keep the surface wet until the numbers can be recorded and photographed. A hand lens or a stereomicroscope were the only microscopical tools necessary. I know that this description is sketchy, but it should give you a starting point. Also, keep in mind that if the person who was trying to remove the serial number knew his business, he (or she) may have ground deeply enough to obliterate any trace of the number and no method will restore it.
Regards, Bill Roberts
erich-at-ento.csiro.au (Eric Hines) on 11/06/2000 02:46:04 AM
To: Microscopy-at-sparc5.microscopy.com cc: (bcc: William H Roberts/US/FILM/DPT)
Dear all, We have been asked to visualize the manufacturers stamped numbers on a rifle which have been removed by grinding. So far secondary and backscatter images don't tell us much. We are told that the metal under the stamping is compressed for a significant depth. Any ideas on how to image this compressed metal. Cheers, Eric Hines Microscopy Centre CSIRO Entomology Canberra OZ
Light optical metallographic techniques, rather than electron optical, might serve you in this case.
If you polish the region - removing as little material as possible, but through 6 micron diamond anyway - and etch with 2% nital (2% nitric acid in alcohol) - you ought to reveal the underlying numbers. Other people may suggest more effective etchants for this purpose; nital is just a general purpose old standby.
Regards, Andrew T. Werner Shaped Charge Research - Metallurgy Laboratory Schlumberger Reservoir Completions Technology Center 14910 Airline Road, Rosharon, TX 77583-1590 Voice (281) 285-5272 Fax (281) 285-5273
"We shoot the hippopotamus with bullets made of platinum 'cause if we used the leaden ones his hide would surely flatten 'em" - Author Unknown
At 06:46 PM 11/06/2000 +1100, Eric Hines wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
One of my users has just asked me for references regarding the technique of acquiring electron induced color CL images by obtaining RGB channels via red-blue-green filters. One of those procedures we have simply been taking for granted, but now want to acknowledge earlier work, especially with respect to geologic materials and quartz.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
If you can submerge the specimen under a liquid it will greatly enhance the captive effects from 3 or so for most fluids to 70 for water due to the dielectric properties of the liquid.
I have no idea if this is possible but if it is you can get a great deal of improvement in resolution in differences in height. Cracks in particular would be highlighted.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 } From: "Tim Akinbo" {takinbo-at-onebox.com} } } Greetings. } I am a final student of the department of Electrical Electronics, } Obafemi Awolowo University, Ile-Ife, Nigeria. } I am doing a project titled "Low-Frequency Surface Microscopy". My } supervisor wants me to use an electric field-probe to detect changes in } the capacitance of the surface as cracks, boundaries, etc. are } encountered. I would be very grateful if you can send me any } information on the topic. } } My e-mail address is: femi_adeluyi-at-yahoo.com and my address is: } Olufemi Adeluyi } Department of Electronic and Electrical Engineering } Obafemi Awolowo University, } Ile-Ife, Nigeria. } } Thanking you in anticipation. } }
Maria Mendes wrote: ========================================== I am a research lab that does a lot of hard tissue processing, I as well as our EM departments have been using SPURR as our plastic of choice. I have been recently told by my supplier (Marivac) that in six months one of the components of SPURR will be discontinued. The component is ERL (Vinylcyclohexene Dioxide). I would like to know if others are using SPURR and if so have they encountered the same problem. Please forward your information to mmendes-at-mtsinai.on.ca. ========================================= This posting generated some worried communcations with customers asking about the continued availability of ERL 4221 (Vinylcyclohexene Dioxide).
The manufacturer reports to us no plans to discontinue ERL 4221 and there seems to be enough in the distribution pipelines to last some years into the future. ERL 4221 is currently available from SPI Supplies as I suspect it is available also from the other major suppliers of chemicals to the microscopy world.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
Depending upon the steel the receiver is made from a Fry's or Modified Fry's reagent (a cupric Chloride in HCl solution) works much better than the HNO3 but both alternated works still better. As I said in a previous post on this the NASA publication has quite a good rundown on all of the appropriate solutions. Hatcher, Jury and Weller a less detailed but acceptable section on it. The details of how to prepare the metal is as important as the solutions chosen if it is to work. Drop me a note and I'll write the procedure up if you can't find the NASA publication.
Jim Roberts
James L. Roberts Firearm and Toolmark Examiner Ventura Co. Sheriff's Lab (805) 654-2308 James.Roberts-at-mail.co.ventura.ca.us
} } } "Purdy, Sam" {SPurdy-at-nationalsteel.com} 11/06/00 05:42AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Eric:
Some forensic scientists have been successful in detecting stamped serial numbers by etching the part in a solution of 5 -10% HNO3 in water or alcohol for 10 to20 seconds. The disturbed metal will etch more darkly than the surrounding metal.
Best regards and good luck,
Sam Purdy Tecnical Center National Steel Corp. Trenton MI spurdy-at-nationalsteel.com
Experienced Confocal Microscopy/Electron Microscopy Technologist to work in Research Laboratory of Department of Pathology
The person we seek will be responsible for organization of a new facility that includes a confocal microscope and an electron microscope. The position includes overall management of the microscopy facility, user training, and user supervision. Requirements for the position include experience with light and transmission electron microscopy. This individual will oversee all aspects of specimen accession and processing, operation of the microscopes, photography, record keeping, and supervision of a technician. Knowledge of EM, biology, and pathology, as well as photographic procedures is required. The individual will work with only minimum supervision.
Position Requirements
Experience with confocal and digital imaging techniques, microinjection, visualization of living cells containing fluorescent probes, photobleaching, and fluorescence in situ hybridization.
Minimum of five years experience working in an electron microscopy laboratory with hands-on experience in tissue processing, dark room photography, and operation and maintenance of electron microscope.
Two years experience working on electron microscopy of human tissue specimens with knowledge of histology and pathology.
Excellent interpersonal and organizational skills are essential.
BachelorUs degree in science desirable.
Desirable Experience
Expertise in training in the operation of confocal microscope systems is a distinct advantage.
Familiarity with light microscopy methods, immunofluorescent staining, use of fluorescent probes for physiologic measurements and the general principles of cell biological research are critical. Significant facility with computers is desired.
Responsibilities
Serve as the technical manager of the facility and be responsible for the operation and maintenance of the confocal and EM microscope facility.
Perform routine transmission EM, including tissue processing, ultramicrotomy, and examination; do preventative maintenance on the equipment; maintain the lab, order supplies, schedule instruments, and oversee billing.
Image analysis at the light, confocal and electron microscopic levels and preparation of micrographs for publication.
Send resume to: Suzanne Mirra, M.D., Chair Department of Pathology Box 25 SUNY-Brooklyn 450 Clarkson Av. Brooklyn, NY 11203-2098
----------------- Submitted by Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Experienced Histotechnologist to work in Neuropathology Research Laboratory of Department of Pathology
The person we seek will be responsible for maintaining a neuropathology research laboratory in the Department of Pathology at SUNY Downstate Medical Center. Knowledge of immunohistochemistry, special stains, and histopathology is required. Previous experience in handling central nervous system tissue preferred. The individual will work with only minimum supervision.
Position Requirements
Minimum of five years experience working in a laboratory with hands-on experience in tissue processing, histochemistry, immunohistochemistry, and operation of a light microscope. Experience with technical aspects of neuropathology and performing special stains including silver (Bielschowsky) and myelin stains.
Two years experience working on immunohistochemistry of CNS tissue specimens with knowledge of immunoreagents and experimental protocols.
BachelorUs degree in science desirable.
Laboratory skills including communications, ability to comply with safety and laboratory regulations, maintenance of laboratory equipment and resources, operation of computers and office equipment.
Advanced computer skills (word processing and database management) essential.
Desirable Experience
Confocal microscopy desirable.
Salary commensurate with experience
Responsibilities
Purchasing supplies and equipment, budget reports, laboratory maintenance and brain banking.
Will operate all microscopic, photographic and computer equipment, and keep accurate records of all laboratory experiments and procedures. Light and fluorescent microscopy; tissue processing for paraffin embedding, sectioning and slide stainer for immunohistochemical procedures; computer imaging (PhotoShop); general photography; and library and web searches
Familiarity with computer software for keeping records, report preparation and table/figure construction using Microsoft Office software (Word, Excel, PowerPoint) as well as Endnote and Adobe PhotoShop.
Send resume to:
Suzanne Mirra, M.D., Chair Department of Pathology Box 25 SUNY-Brooklyn 450 Clarkson Av. Brooklyn, NY 11203-2098
------------------- Submitted by Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Experienced Electron Microscopy Technologist to work in Research Laboratory of Department of Pathology
The person we seek will be responsible for the overall operation of the EM laboratory in the Department of Pathology at SUNY Downstate Medical Center. This individual will oversee all aspects of specimen accession and processing, operation of the microscope, photography, record keeping, and supervision of a technician. Knowledge of EM, biology, and pathology, as well as photographic procedures is required. The individual will work with only minimum supervision.
Position Requirements
Minimum of five years experience working in an electron microscopy laboratory with hands-on experience in tissue processing, dark room photography, and operation and maintenance of electron microscope.
Two years experience working on electron microscopy of human tissue specimens with knowledge of histology and pathology.
BachelorUs degree in science desirable.
Laboratory management skills including effective written/verbal communication skills to interact with a diverse group, ability to comply with safety and laboratory regulations, maintenance of laboratory equipment and resources, and operation of computers and office equipment.
Desirable Experience
Previous experience in confocal microscopy highly desirable.
Previous experience in performing immunocytochemical staining and advanced computer skills usage (e.g. image analysis) is also desirable.
Salary commensurate with experience
Responsibilities
Maintain electron microscope in operating condition. Process clinical and research tissues for "thick" and "thin" sectioning. Darkroom management of photographic printing.
Send resume to: Suzanne Mirra, M.D., Chair Department of Pathology Box 25 SUNY-Brooklyn 450 Clarkson Av. Brooklyn, NY 11203-2098
----------------- Submitted by: Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
In many of the microscope rooms here at ANL. we use a product called SONEX by illbruck (http://www.illbruck.com). It is both fire rated and works reasonably well. Log into the TPM WWW site (http://tpm.amc.anl.gov) and you can see it . It is an acoustic foam panel which glues to the walls of the lab. The only problem is that being a foam product it tends to easily tear/rip if it is hit by objects.
Nestor
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================================================================== Dr. Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov ================================================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ==================================================================
The box said "This program requires Win 95/98/NT or better..." So I bought a G3 Mac !
} We are going to install a curtain for sound absorption } in our TEM Lab. Is there any special curtain for this } purpose?
What sort of noise? For mid- to high-frequencies, a heavy fabric drape, interlined with an absorbent material, will do a pretty good job. For low frequencies, no curtain will do as you need a membrane with a seal.
On Mon, 6 Nov 2000, Nestor J. Zaluzec wrote: } In many of the microscope rooms here at ANL. we } use a product called SONEX by illbruck (http://www.illbruck.com). It is both fire } rated and works reasonably well. Log into the TPM WWW site } (http://tpm.amc.anl.gov) and you can see it . It is an acoustic foam panel which } glues to the walls of the lab. The only problem is that being a foam } product it tends to easily tear/rip if it is hit by objects.
This will do nothing to keep sound out. It will absorb high frequencies within the room.
Better sites for information and materials are Acoustic Sciences Corporation and Auralex.
Hello everyone, I was wondering if anyone had suggestions on a protocol for the fixation of either aquatic plant tissue or fish tissue to be viewed in the SEM. My interest is to study the sorption mechanisms of heavy metals (Cr, Cd and Pb) by aquatic plants and the bioaccumulation in fishes. I have no experience on SEM, so any information will be very usefull for me. Thanks, Regards!
Hi all, I would appreciate any suggestions or comments on how to suppress fluorescence of collagen in frozen sections that have been subjected to indirect immunofluorescence using Alexa conjugated antibodies. I have used normal goat and normal horse serum to block non-specific staining as well as 4 % fish gelatin but the collagen glows brightly every time. the samples were fixed in cold acetone before blocking. Incubation with sodium borohydride did not reduce fluorescence.
Thanks,
Cora Bucana Corazon D. Bucana, Ph.D. Dept. Cancer Biology UT M.D.Anderson Cancer Center 1515 Holcombe Blvd, Box 173 Houston, Texas 77030 Phone: 713-792-8106 FAX 713-792-8747
Kal could you expand on this a little? What type of membrane, arranged how?, etc. What range of frequencies can be controlled this way? I am looking for a relatively straightforward and inexpensive way of controlling particularly the low end of the spectrum of acoustic frequencies (generated by human voice, RVPs, etc.) in an FESEM lab. Chris
} For low frequencies, no curtain will do } as you need a membrane with a seal. } } Kal } }
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
I have found that if I keep some scraps around after the original installation of almost anything you can mix up a little glue wiht it and make a passable fix. Some times you have to do a little painting and make a creative dirty spot.
You will always be able to see it but no one else will.
If you are worried about getting the texture right use cheap wall paper paste and you can wash it out if you don't like it but it has enough water resistance to resist minor spills.
Test it where it won't show first.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 } } In many of the microscope rooms here at ANL. we } use a product called SONEX by illbruck (http://www.illbruck.com). It is both fire } rated and works reasonably well. Log into the TPM WWW site } (http://tpm.amc.anl.gov) and you can see it . It is an acoustic foam panel which } glues to the walls of the lab. The only problem is that being a foam } product it tends to easily tear/rip if it is hit by objects. } } Nestor } } } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Dear Listers, } } } } We are going to install a curtain for sound absorption in our TEM Lab. Is there any special curtain for this purpose? } } } } Regards, } } } } Kun Li } } } } } } Get your FREE Email at http://www.mailcityasia.com } } } ================================================================== } Dr. Nestor J. Zaluzec } Materials Science Division } Building 212 } Argonne National Lab } 9700 S. Cass Ave } Argonne, Illinois 60439 USA } Tel: 630-252-7901, Fax: 630-252-4798 } Email: Zaluzec-at-aaem.amc.anl.gov } ================================================================== } TPMLab: http://tpm.amc.anl.gov } MMSite: http://www.amc.anl.gov } ================================================================== } } The box said "This program requires Win 95/98/NT or better..." } So I bought a G3 Mac ! } } ================================================================== } } }
We are experimenting with apoptose. Now, we want to view apoptotic cells under the SEM. Has anyone ideas what technique, solutions,....we have to use ? The sample is canine ovary.
Thx.
Bart De Pauw Ghent University Faculty of Veterinary medicine Department Morphology Salisburylaan 133 9820 Merelbeke Belgium
Description of Duties: Works with the Coordinator of Research Programs/Services in the management of the day-to-day activities of the Biological Science Imaging Resource, a campus wide imaging laboratory. This facility is responsible for assisting faculty and students in the preparation of biological images for research, publication and instruction. The Senior Biological Scientist works closely with the Coordinator to identify facility needs, establish priorities and develop policies within the laboratory as well as instruct users in the operation of the electron and light, including confocal microscopes. The incumbent must stay abreast of research standards and practices to maintain quality control and advise users on appropriate protocols. Knowledge in the use of MetaMorph, MetaFluor, Photoshop and PowerPoint along with MAC and PC platforms is a plus.
Contact Kim Riddle, 119 Bio Unit I, Department of Biological Science, Florida State University, Tallahassee, FL 32306-4370, riddle-at-bio.fsu.edu 850.644.6519 Imaging Resource web page http://bio.fsu.edu/~taylor/imaging On-line applications available at http://personnel.fsu.edu/emply/homepage.html
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Kimberly A. Riddle Florida State University tel: 850.644.6519 Biological Science Imaging Resource 119 Bio Unit I, 4370 fax: 850.644.0481 Tallahassee, FL 32306 riddle-at-bio.fsu.edu http://bio.fsu.edu/~taylor/imaging ~~~~~~~~~~~~~~~~~~~~~~~~~~~~
The Center for Materials Research and Analysis (CMRA) at the University of Nebraska at Lincoln has a matching fund in connection with a NSF award for a person to work with Prof. Y. Liu and his co-PIs on image processing of high resolution electron micrographs. The method developed will be applied to the TEM characterization of various nanostructured materials, such as TiN coatings, nanowires, super-conducting materials, recording media and permanent magnets. The initial appointment will be one year and can be renewed the second year, depending upon progress and availability of funding. Computer skills, knowledge of Fourier transform and image processing are required. Interested persons should email a letter of interest, resume and three persons of references to yliu-at-unlserve.unl.edu-- ******************************************************************* Yi Liu Department of Mechanical Eng. and CMRA 104 N Walter Scott Engineering Center University of Nebraska-Lincoln Lincoln, NE 68588-0656 Tel. (402) 472-7759 (Office) Tel. (402) 472-8762 (EM lab) Fax (402) 472-1465 Email: yliu-at-unlserve.unl.edu *******************************************************************
} could you expand on this a little? What type of membrane, arranged } how?, etc. What range of frequencies can be controlled this way? } I am looking for a relatively straightforward and inexpensive way of } controlling particularly the low end of the spectrum of acoustic } frequencies (generated by human voice, RVPs, etc.) in an FESEM } lab.
Assuming that you want to keep external noise out, you need to block the window since the glass transmits a lot. Hanging drapes, of any kind, will be most effective at higher frequencies where they can absorb energy transmitted by the glass.
If you can seal over the window with a membrane that will absorb at low frequencies (and you must seal it for it to be effective at low frequencies), take a look at SheetBlok at http://www.auralex.com/. This is effective down into the range of about 100Hz. Alternatively, you can brick up the window.
We also use Sonex foam products in most of our laboratories, but are now looking at purchasing an "enclosure" to put around a noisy water chiller. A company called Sound Seal (www.soundseal.com) makes such enclosures (at *very* reasonable prices), and has fabric curtains and panels for sound absorption purposes. Check them out...
Larry
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Dr. Lawrence F. Allard Senior Research Staff Member High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 PO Box 2008 Oak Ridge, TN 37831-6064
You might need to expand a bit on your goal. It seems that you are indicating that human speech is degrading your resolution. I find that hard to believe. Pumps, maybe. All these sources would depend on their manner of coupling to the scope. I don't think that speech would be that effectively coupled to be a problem.
Have you tried collecting some images where part of the image has the source present and part does not? I would think you could even shut down your rotary pumps for long enough to determine their influence on imaging. Take appropriate precautions.
Like the previous discussion on mu metal shielding, it seems that you might want to isolate the problem better and/or try some cheap and crude solutions before investing the bigger bucks.
FWIW, Warren Straszheim
At 07:57 AM 11/7/2000 +0000, you wrote:
} Kal } could you expand on this a little? What type of membrane, arranged } how?, etc. What range of frequencies can be controlled this way? } I am looking for a relatively straightforward and inexpensive way of } controlling particularly the low end of the spectrum of acoustic } frequencies (generated by human voice, RVPs, etc.) in an FESEM } lab. } Chris } } } For low frequencies, no curtain will do } } as you need a membrane with a seal. } } } } Kal } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Dr Chris Jeffree } University of Edinburgh } Biological Sciences EM Facility } Daniel Rutherford Building } King's Buildings EDINBURGH EH9 3JH } Tel: +44 (0) 131 650 5345 } FAX: +44 (0) 131 650 6563
I am interested in acquiring software for 3D image reconstruction and measurement (distance/volume) in 3D space. What is the current state of the market in this area? I am familiar with only one company that provides such a package (Vital Images: VoxelView), but I bet there are others. No need to evangelize - just let me know what other products I should examine. Money is not a big obstacle, I do not want to write code myself (I want a turnkey solution), and I do not require image acquisition or 2D analysis features. Thanks for the input. Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 308 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax 301-402-0396
Speech can indeed be a problem with FESEMs. In the case of our instrument, the shrouding around the ion pumps behind the column was acting as a drum, transmitting speech particularly well. I removed the shrouding and the problem was eliminated. Plus now the scope looks much more interesting and impresses visitors! In newer models from the same company the shrouding is now perforated to minimize the problem.
} You might need to expand a bit on your goal. It seems that you are } indicating that human speech is degrading your resolution. I find that hard } to believe. Pumps, maybe. All these sources would depend on their manner of } coupling to the scope. I don't think that speech would be that effectively } coupled to be a problem.
Aloha, Tina
Mid-80s F, sunny with tropical breezes
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Yes I am indicating exactly that. Certain frequencies of the human voice at normal conversational amplitudes are picked up by FESEM columns and can induce saw-tooth edges on sharp-edged features (such as gold crystals on carbon) at magnifications in the x100k region. Trust me on this. It is a real problem. If one is contemplating high resolution work with an FESEM a hushed environment is essential.
Chris
} It seems that you are } indicating that human speech is degrading your resolution. I find that hard } to believe. Pumps, maybe. All these sources would depend on their manner of } coupling to the scope. I don't think that speech would be that effectively } coupled to be a problem. } } Have you tried collecting some images where part of the image has the } source present and part does not? I would think you could even shut down } your rotary pumps for long enough to determine their influence on imaging. } Take appropriate precautions. } } Like the previous discussion on mu metal shielding, it seems that you might } want to isolate the problem better and/or try some cheap and crude } solutions before investing the bigger bucks. } } FWIW, } Warren Straszheim } } At 07:57 AM 11/7/2000 +0000, you wrote: } } } Kal } } could you expand on this a little? What type of membrane, arranged } } how?, etc. What range of frequencies can be controlled this way? } } I am looking for a relatively straightforward and inexpensive way of } } controlling particularly the low end of the spectrum of acoustic } } frequencies (generated by human voice, RVPs, etc.) in an FESEM } } lab. } } Chris } } } } } For low frequencies, no curtain will do } } } as you need a membrane with a seal. } } } } } } Kal } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } Dr Chris Jeffree } } University of Edinburgh } } Biological Sciences EM Facility } } Daniel Rutherford Building } } King's Buildings EDINBURGH EH9 3JH } } Tel: +44 (0) 131 650 5345 } } FAX: +44 (0) 131 650 6563 } }
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
I have 2 Pfeiffer's no-oil vacuum pumps for 8m3/min to offer . They have only 50hours of work. The original price the last year was $6400. Any offer negotiable. Marek Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories University of California, San Diego
Warren E Straszheim: } } You might need to expand a bit on your goal. It seems that you are } indicating that human speech is degrading your resolution. I find that hard } to believe.
Dear Warren, I still remember Ken Downing demonstrating that his IVEM image would vibrate whenever one talked at the microscope--in fact, at a DOE site visit, the head of the team, a Dr. Happer, seemed singularly unimpressed until he was shown that clapping in the IVEM room would cause the image to vibrate. He was apparently quite amused, and the site visit went well after that. The IVEM was known for a time as the "Happer clapper". Yours, Bill Tivol Wadsworth Center Albany NY
I have to back Chris up on this. We have often seen voice-induced vibration in our FESEM. No talking during high-mag, high-res work.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
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Yes I am indicating exactly that. Certain frequencies of the human voice at normal conversational amplitudes are picked up by FESEM columns and can induce saw-tooth edges on sharp-edged features (such as gold crystals on carbon) at magnifications in the x100k region. Trust me on this. It is a real problem. If one is contemplating high resolution work with an FESEM a hushed environment is essential.
Chris
} It seems that you are } indicating that human speech is degrading your resolution. I find that hard } to believe. Pumps, maybe. All these sources would depend on their manner of } coupling to the scope. I don't think that speech would be that effectively
} coupled to be a problem. } } Have you tried collecting some images where part of the image has the } source present and part does not? I would think you could even shut down } your rotary pumps for long enough to determine their influence on imaging.
} Take appropriate precautions. } } Like the previous discussion on mu metal shielding, it seems that you might } want to isolate the problem better and/or try some cheap and crude } solutions before investing the bigger bucks. } } FWIW, } Warren Straszheim } } At 07:57 AM 11/7/2000 +0000, you wrote: } } } Kal } } could you expand on this a little? What type of membrane, arranged } } how?, etc. What range of frequencies can be controlled this way? } } I am looking for a relatively straightforward and inexpensive way of } } controlling particularly the low end of the spectrum of acoustic } } frequencies (generated by human voice, RVPs, etc.) in an FESEM } } lab. } } Chris } } } } } For low frequencies, no curtain will do } } } as you need a membrane with a seal. } } } } } } Kal } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } Dr Chris Jeffree } } University of Edinburgh } } Biological Sciences EM Facility } } Daniel Rutherford Building } } King's Buildings EDINBURGH EH9 3JH } } Tel: +44 (0) 131 650 5345 } } FAX: +44 (0) 131 650 6563 } }
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
} Yes I am indicating exactly that. Certain frequencies of the human } voice at normal conversational amplitudes are picked up by FESEM } columns and can induce saw-tooth edges on sharp-edged features } (such as gold crystals on carbon) at magnifications in the x100k } region. Trust me on this. It is a real problem. If one is contemplating } high resolution work with an FESEM a hushed environment is } essential.
OK. Where are these voices? In the room? If not, the issue is to keep them out. Perhaps you should contact a number of the specialist firms and consult with them via their websites. I have gotten very useful advice and information without (or prior to) purchase of sound treatment materials.
Kal
} } It seems that you are } } indicating that human speech is degrading your resolution. I find that hard } } to believe. Pumps, maybe. All these sources would depend on their manner of } } coupling to the scope. I don't think that speech would be that effectively } } coupled to be a problem. } } } } Have you tried collecting some images where part of the image has the } } source present and part does not? I would think you could even shut down } } your rotary pumps for long enough to determine their influence on imaging. } } Take appropriate precautions. } } } } Like the previous discussion on mu metal shielding, it seems that you might } } want to isolate the problem better and/or try some cheap and crude } } solutions before investing the bigger bucks. } } } } FWIW, } } Warren Straszheim } } } } At 07:57 AM 11/7/2000 +0000, you wrote: } } } } } Kal } } } could you expand on this a little? What type of membrane, arranged } } } how?, etc. What range of frequencies can be controlled this way? } } } I am looking for a relatively straightforward and inexpensive way of } } } controlling particularly the low end of the spectrum of acoustic } } } frequencies (generated by human voice, RVPs, etc.) in an FESEM } } } lab. } } } Chris } } } } } } } For low frequencies, no curtain will do } } } } as you need a membrane with a seal. } } } } } } } } Kal } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } } Dr Chris Jeffree } } } University of Edinburgh } } } Biological Sciences EM Facility } } } Daniel Rutherford Building } } } King's Buildings EDINBURGH EH9 3JH } } } Tel: +44 (0) 131 650 5345 } } } FAX: +44 (0) 131 650 6563 } } } } } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Dr Chris Jeffree } University of Edinburgh } Biological Sciences EM Facility } Daniel Rutherford Building } King's Buildings EDINBURGH EH9 3JH } Tel: +44 (0) 131 650 5345 } FAX: +44 (0) 131 650 6563 } } Inveresk Cottage, 26 Carberry Road, } Inveresk, Musselburgh, Midlothian EH21 8PR, UK } Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401 } FAX: +44 (0) 131 653 6248 } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ }
Well, I guess I learned something today. I usually show operators that loud noises will show up as low energy noise in the EDS spectra. I had no idea that it also affected the images that much. We do not do that much work above 10 kx. I will have to bear that in mind the next time we venture into that realm and turn down the 1812 Overture in the background. I may have to quiet our pumps down too.
Warren
At 07:59 PM 11/7/2000 +0000, you wrote: } Yes I am indicating exactly that. Certain frequencies of the human } voice at normal conversational amplitudes are picked up by FESEM } columns and can induce saw-tooth edges on sharp-edged features } (such as gold crystals on carbon) at magnifications in the x100k } region. Trust me on this. It is a real problem. If one is contemplating } high resolution work with an FESEM a hushed environment is } essential. } } Chris
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } On Tue, 7 Nov 2000, Chris Jeffree wrote: } } } Yes I am indicating exactly that. Certain frequencies of the human } } voice at normal conversational amplitudes are picked up by FESEM } } columns and can induce saw-tooth edges on sharp-edged features } } (such as gold crystals on carbon) at magnifications in the x100k } } region. Trust me on this. It is a real problem. If one is contemplating } } high resolution work with an FESEM a hushed environment is } } essential. } } OK. Where are these voices? In the room? If not, the } issue is to keep them out. Perhaps you should contact a } number of the specialist firms and consult with them via } their websites. I have gotten very useful advice and } information without (or prior to) purchase of sound } treatment materials. } } Kal } (snip)
I'd like to jump into this one by adding my $0.02. I'm familiair with Ken Downing's results related to vibrations. We tried it ourselves as well and microphonics are indeed a very real problem when one does HR work. The effects are indeed very clearly visible on the TV screen at high (} = 100kx) mag. Ken (together with Bob Glaeser) and we (perhaps others have done something similar) have taken this a step further and constructed boxes made of styrofoam that fit around the cryo-holder (side entry types). The idea was to keep microphonics away from the holder including those created by us talking or on the phone or listening to a radio, but also those originating from pumps, ventilation and/or air conditioning systems.
I operate a Noran Voyager 3.3 EDS system, which is a UNIX based system and operates on a Sun Sparc Station 5 with SunOS 5.3. The problem is the local IT department is reluctant to place this on the network which is all PC based. The IT staff feels it would be more cost efficient use of their time, if I found any product which will replace the Unix box/software and utilize the present hardware (detector, amplifiers, etc.). Anyone have any experience with this?
TIA,
Dave Audette OSRAM Sylvania 71 Cherry Hill Drive Beverly, MA 01915 david.audette-at-sylvania.com
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Does anybody know of any used CCD camera and EDS in good condition that are up for sail? I would like to purchase these to use on a used JEOL 200CX TEM.
Your help would be greatly appreciated.
Regards, Thearith
_________________________
Thearith Ung, Ph.D. Quantum Dot Corporation 26136 Research Road Hayward CA 94545 Tel: +1-510-887-8775 (x4125) Fax:+1-510-783-9729 www.qdots.com
} I operate a Noran Voyager 3.3 EDS system, which is a } UNIX based system and operates on a Sun Sparc Station } 5 with SunOS 5.3. The problem is the local IT } department is reluctant to place this on the network } which is all PC based. The IT staff feels it would be } more cost efficient use of their time, if I found any } product which will replace the Unix box/software and } utilize the present hardware (detector, amplifiers, } etc.). Anyone have any experience with this?
Your IT department is over simplifying the cost. They most likey think it's only 1 or 2k to get a WinOS computer. They are very wrong.
Cost is really going to be from ~10k to ~20k to replace the Voyager depending if you go with a Noran (Quest) replacement or some other like us. You will be able to keep the detector (although maybe a preamp change) but everything else is replaced. Also you will have to learn new EDS software (your time at two to three weeks). In addition, all your old data and analysis might or might not be movable to the new EDS system (how much is your data worth).
Tell your managment that your IT department needs to to support you rather than you supporting them, after all, what is the function of the TI department. The Voyager is a plain UNIX box, if no one in the IT department understands UNIX enough to network it, they are pretty sorry. If that does not work, then tell then you will be glad to replace the UNIX with a WinOS equivalent provided they pony up the funds. Also remind managment that you will get no real work done while you learn the new software. Add the fact that previous data results might be forever lost to access. Let them figure that the IT deparment is going to cost the company 10-20k plus the cost of your time because they can't seem to do their job which is to support the computing needs of the workers. Bottom line is that it's going to cost the company 40-60k direct and indirect costs however if you can't move the data, then it could cost the company big time if they need to access it in the future. Now what is really more cost efficient?
Sorry for the rant, but it really bugs me when IT departments operate in such a priesthood fashion, we run into it all the time with our MacOS products so much that we came out with WinOS products for those that have to have WinOS because of stupid IT restrictions.
Scott ----------------------------------------------------------------------- Scott D. Davilla Phone: 919 489-1757 ext 13 (tel) 4pi Analysis, Inc. Fax: 919 489-1487 (fax) 3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com Durham, North Carolina 27707-2534 web: http://www.4pi.com
Huh? What PCs? If they are all DOS-based, I can identify with their concerns. If the PCs are running client TCP/IP, the addition of the Sun should be trivial.
How are LAN IPs assigned? If there is a LAN NAT server, then just assign the Sun a within-scope IP address. If it is DHCP, just do it.
I don't see what the big deal is. Except....I'll venture to say that the PC jockeys have no Unix experience. That would be a good reason to keep the Sun away from the PC LAN.
gg
At 04:36 PM 11/7/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} From: "Scott D. Davilla" {davilla-at-4pi.com} } } } I operate a Noran Voyager 3.3 EDS system, which is a } } UNIX based system and operates on a Sun Sparc Station } } 5 with SunOS 5.3. The problem is the local IT } } department is reluctant to place this on the network } } which is all PC based. The IT staff feels it would be } } more cost efficient use of their time, if I found any } } product which will replace the Unix box/software and } } utilize the present hardware (detector, amplifiers, } } etc.). Anyone have any experience with this? =========== Just tell them you can't get fries wiht that and do their job. And don't hold your breath. If you ask around there may be a couple working wiht Linux and they can figure it out.
Just borrow some one with unix experiance to set it up from another department for a favor to be anouced later and let the IT department spin on it. If they are windows based the box will weld it's self shut by molecular defusion before they take the time to figure out unix.
Hiring a consultant is a reasonable soluting if a college is near. Once it is set up it can be maintained from any place in the world. Unless you are adding stuff a couple of hours a month will do the job.
The best answer is some one in the shop learn to do it. It will take some time but it will pay off in he long run.
Good luck You need it. Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
} From: "William Tivol" {tivol-at-wadsworth.org} } } Warren E Straszheim: } } } } You might need to expand a bit on your goal. It seems that you are } } indicating that human speech is degrading your resolution. I find that hard } } to believe. } } Dear Warren, } I still remember Ken Downing demonstrating that his IVEM } image would vibrate whenever one talked at the microscope--in fact, } at a DOE site visit, the head of the team, a Dr. Happer, seemed } singularly unimpressed until he was shown that clapping in the } IVEM room would cause the image to vibrate. He was apparently } quite amused, and the site visit went well after that. The IVEM } was known for a time as the "Happer clapper".
Noise is a problem of inches. The best solution is a corn field 5 miles from any where in a 10 foot basment on a still night and a active noise canceler makes it better. We put our insterments in buildings with people and macinery. I was testing a simple load cell with a 12 bid A/D converter. It was no trick to measure a car drive by 30 yards away a truck 100 yards a way a fellow walking by in the hall. And a load cell with a 12 bit a/d converter is not a very sensitive insterment.
There are good solutions down to 4 or 5 Hz below that you have trouble. Floating it on an air table gets x and y but not z. floating it in liqid work until you get a resonate situation. Active cancelation works the best and costs the most. The things tried on this list sows that it is a difficult problem.
You live or die by the contact with your coustomers so you lab has to be near them. That usualy means near traffic and a lot of people. and a lot of vibration and noise. Call your favirate noise reduction vendor and say fixit I have this much money or go to you customers hat in hand and get what it takes to do it right.
Good luck Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
I had the same system when I worked with ESA. I don't remember much difficulty with SunOS 5.3. We just gave it a name & IP address on our LAN, checked a few boxes in the set-up screen & off we went. I don't think we even needed to call in the IT people.
If your IT people can set up a DOS box, they can set up SunOs 5.3. Should be a 5-10 minute job, longer if they have to RTFM.
Regards
Tim
***************************************************************** Tim E. Harper Managing Director CMP Cientifica s.l. Space & NanoTechnology Division Phone +34 91 640 71 85 Fax +34 91 640 71 86 http://www.cmp-cientifica.com/
-----Original Message----- } From: Gary Gaugler [mailto:gary-at-gaugler.com] Sent: Wednesday, November 08, 2000 6:24 AM To: Dave Audette Cc: MSA listserver
Huh? What PCs? If they are all DOS-based, I can identify with their concerns. If the PCs are running client TCP/IP, the addition of the Sun should be trivial.
How are LAN IPs assigned? If there is a LAN NAT server, then just assign the Sun a within-scope IP address. If it is DHCP, just do it.
I don't see what the big deal is. Except....I'll venture to say that the PC jockeys have no Unix experience. That would be a good reason to keep the Sun away from the PC LAN.
gg
At 04:36 PM 11/7/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} From: "Gary Gaugler" {gary-at-gaugler.com} } } Huh? What PCs? If they are all DOS-based, I can identify } with their concerns. If the PCs are running client TCP/IP, } the addition of the Sun should be trivial. } } How are LAN IPs assigned? If there is a LAN NAT } server, then just assign the Sun a within-scope IP address. } If it is DHCP, just do it. } } I don't see what the big deal is. Except....I'll venture } to say that the PC jockeys have no Unix experience. } That would be a good reason to keep the Sun away } from the PC LAN.
No it is a good reason to keep it away from the people runing the LAN. The Lan it's self doesn't care what is hooked to it. The fool that trys to hook it up has a lot to do wiht it. Back up everything before the come and after they leave. So you can go back to a working state not connected to the lan.
I can't get Unix jocks for my own projects good luck on yours. And one is my son.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
I would echo the sentiments you have already received. It seems like your IT folks just have no experience with UNIX. (But neither do I.) It would take them some time to learn it, even if they are willing.
It absolutely should be a trivial matter to put a Unix box on a TCP/IP network, and that is all you should need. If they are thinking that they have to run Novell or some other protocol on your Unix system, that could be a very difficult prospect. But that is not what you would want. All you need is TCP/IP and Unix is where TCP/IP got its start.
I would suggest that you get the rules for setting up an additional PC node on the network. Let them give you an IP number and name or tell you that your net runs a DHCP server to hand out IP numbers. Hey, you could even give them an old, dummy PC and say hook this up to the net and call it such and so. Then you could have someone who does know Unix and Windows come in, look over the setup, transfer the setting to the Unix box, and dispose of the dummy PC.
In other words, it really should not be that tough. I bet you have someone around that could do it. That is the way I helped another lab on campus get their PDP-based Kevex EDS system onto the net. I had already done it with ours.
Good luck.
Warren S.
At 04:36 PM 11/7/2000 -0800, you wrote: } Hello listers, } } I operate a Noran Voyager 3.3 EDS system, which is a } UNIX based system and operates on a Sun Sparc Station } 5 with SunOS 5.3. The problem is the local IT } department is reluctant to place this on the network } which is all PC based. The IT staff feels it would be } more cost efficient use of their time, if I found any } product which will replace the Unix box/software and } utilize the present hardware (detector, amplifiers, } etc.). Anyone have any experience with this? } } TIA, } } Dave Audette } OSRAM Sylvania } 71 Cherry Hill Drive } Beverly, MA 01915 } david.audette-at-sylvania.com } } } } __________________________________________________ } Do You Yahoo!? } Thousands of Stores. Millions of Products. All in one Place. } http://shopping.yahoo.com/
Our experience is exactly the same as Tim's. Ask your IT guys what it is that is peculiar about your network that precludes being able to run a unix box on it. We followed instructions provided by some user friendly unix experts and set up our Voyagers on the network ourselves. I've got the notes on how to do it and could send them to you if you want. The instructions are pretty straightforward,ie the commands to use to get to the setup screen, optional files to create and edit if you want additional networking functions, and so on.
Maybe best if you can persuade the IT guys to assign you with a permanent IP address. From there you shouldn't really need them for anything else. Any other site specific info that you need should be obtainable by looking at the network setup of any PCs (or Macs!) running nearby.
Maybe it's a 15 minute job to do once, 5 minutes if you can avoid the damn FM.
Regards, Arthur
} } I had the same system when I worked with ESA. } I don't remember much difficulty with SunOS 5.3. We just gave it a name & IP } address on our LAN, checked a few boxes in the set-up screen & off we went. } I don't think we even needed to call in the IT people. } } If your IT people can set up a DOS box, they can set up SunOs 5.3. Should be } a 5-10 minute job, longer if they have to RTFM. } } Regards } } Tim } } } ***************************************************************** } Tim E. Harper Managing Director } CMP Cientifica s.l. } Space & NanoTechnology Division } Phone +34 91 640 71 85 Fax +34 91 640 71 86 } http://www.cmp-cientifica.com/ } } -----Original Message----- } } From: Gary Gaugler [mailto:gary-at-gaugler.com] } Sent: Wednesday, November 08, 2000 6:24 AM } To: Dave Audette } Cc: MSA listserver } Subject: Re: EDS networking } } At 04:36 PM 11/7/00, you wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hello listers, } } } } I operate a Noran Voyager 3.3 EDS system, which is a } } UNIX based system and operates on a Sun Sparc Station } } 5 with SunOS 5.3. The problem is the local IT } } department is reluctant to place this on the network } } which is all PC based. The IT staff feels it would be } } more cost efficient use of their time, if I found any } } product which will replace the Unix box/software and } } utilize the present hardware (detector, amplifiers, } } etc.). Anyone have any experience with this? } } } } TIA, } } } } Dave Audette } } OSRAM Sylvania } } 71 Cherry Hill Drive } } Beverly, MA 01915 } } david.audette-at-sylvania.com } } } } } } } } __________________________________________________ } } Do You Yahoo!? } } Thousands of Stores. Millions of Products. All in one Place. } } http://shopping.yahoo.com/
Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457 Ansto Materials Division Fax: 61-2-9543-7179 PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au Australia www: http://www.ansto.gov.au/
At 04:36 PM 11/7/00 -0800, Dave Audette wrote: } I operate a Noran Voyager 3.3 EDS system, which is a } UNIX based system and operates on a Sun Sparc Station } 5 with SunOS 5.3. The problem is the local IT } department is reluctant to place this on the network } which is all PC based.
If you can find anyone there adept in Unix, they would be able to install Samba, a networking package that will make your box look like every other PC in the office in terms of file sharing and printing. You can see their drives or they can see yours.
http://us1.samba.org/samba/samba.html is the start, and http://us5.samba.org/samba/ftp/Binary_Packages/solaris/Sparc/ has binaries or source code for your platform.
Without much information on the networking setup for your lab/company, it shouldn't be that difficult to add it to the existing windows network. You could do it with or without your IT department.
If you have a network line, install a switch/hub, add in your Sun computer to the network, setup its configuration, and you should be able to do simple file transfers via ftp. You could also see if Samba is available for your Sun OS, and then share files more easily.
More information about the network configuration that exists for your company would be needed to give more precise suggestions.
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ Gordon Ante Vrdoljak Electron Microscope Lab ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall gvrdolja-at-nature.berkeley.edu UC Berkeley phone (510) 642-2085 Berkeley CA 94720-3330 fax (510) 643-6207 cell (510) 290-6793
On Tue, 7 Nov 2000, Dave Audette wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello listers, } } I operate a Noran Voyager 3.3 EDS system, which is a } UNIX based system and operates on a Sun Sparc Station } 5 with SunOS 5.3. The problem is the local IT } department is reluctant to place this on the network } which is all PC based. The IT staff feels it would be } more cost efficient use of their time, if I found any } product which will replace the Unix box/software and } utilize the present hardware (detector, amplifiers, } etc.). Anyone have any experience with this? } } TIA, } } Dave Audette } OSRAM Sylvania } 71 Cherry Hill Drive } Beverly, MA 01915 } david.audette-at-sylvania.com } } } } __________________________________________________ } Do You Yahoo!? } Thousands of Stores. Millions of Products. All in one Place. } http://shopping.yahoo.com/ } }
And Hertz. Our HVEM column is very sensitive to 20 Hz, but almost completely insensitive to frequencies a few Hz away on either side. Once you have someone sit at the scope and watch the image as a sound generator is swept through a range of frequencies, you will know what to be concerned about, and then you can get the appropriate shielding.
The best solution is a corn field 5 miles from any where in a 10 foot basment on a still night and a active noise canceler makes it better.
If you build it, will they come? ;-)
We put our insterments in buildings with people and macinery. I was testing a simple load cell with a 12 bid A/D converter. It was no trick to measure a car drive by 30 yards away a truck 100 yards a way a fellow walking by in the hall. And a load cell with a 12 bit a/d converter is not a very sensitive insterment.
There are good solutions down to 4 or 5 Hz below that you have trouble. Floating it on an air table gets x and y but not z. floating it in liqid work until you get a resonate situation. Active cancelation works the best and costs the most. The things tried on this list sows that it is a difficult problem.
There is no free lunch. Floating the instrument renders it sensitive to sound in the room, and mounting it solidly to the floor makes transmission through the ground a problem. If the soil mechanics are such that the frequencies transmitted to the instrument are not those to which it is sensitive, but the sound emitted by the pumps is at the sensitive frequencies, it can be better to bolt the instrument to the floor. As you said, an environment completely free of vibration is best, and active cancellation is good. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
I would like to say thanks to all for the information provided on preparing TEM cross sections of DLC thin films on steel. Also I would like to note that I do have access a FIB tool at Madison Area Technical College, so anyone who has any info on specialized techniques for the TEM prep of DLC thin films using the FIB would greatly be appreciated. Again, thanks to all
Daniel L. Flatoff student Madison Area Technical College
Does anyone have any experience with instituting ISO compliance of SEM's? How do you keep your SEM calibrated and how often are calibrations performed? What type of support does your mfg. offer?
Thank you to those who replied and let me know that their cameras and EDS systems are available for sale. I forgot to ask also what are some of the best vendors that sell CCD cameras and EDS systems? This information will be valuable in helping me to apply for grants to purchase these instruments.
Thank you in advance.
Regards, Thearith _________________________
Thearith Ung, Ph.D. Quantum Dot Corporation 26136 Research Road Hayward CA 94545 Tel: +1-510-887-8775 (x4125) Fax:+1-510-783-9729 www.qdots.com
At 04:36 PM 11/7/2000 -0800, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} From Dave's posting, we can't tell. Before he starts any battles with his IT department, he needs to learn (by his own research or with the help of an advocate) what the reasons are for the IT advice. Maybe he already knows this and didn't include it in his message (but I suspect not, for why would he have posted his message). Without this knowledge, though, the various responses (excepting Scott's) that have been made are not really very helpful.
I know I'm on a soapbox here, and in any forum like this, a major degree of *caveat emptor* has to apply. It is certainly true that, if the OSRAM/Sylvania network is TCP/IP, then the UNIX box is easily networked. If not, though, then Dave will have been misguided, rather than helped, by the responses he has received.
Tony.
} Hello listers, } } I operate a Noran Voyager 3.3 EDS system, which is a } UNIX based system and operates on a Sun Sparc Station } 5 with SunOS 5.3. The problem is the local IT } department is reluctant to place this on the network } which is all PC based. The IT staff feels it would be } more cost efficient use of their time, if I found any } product which will replace the Unix box/software and } utilize the present hardware (detector, amplifiers, } etc.). Anyone have any experience with this? } } TIA, } } Dave Audette } OSRAM Sylvania } 71 Cherry Hill Drive } Beverly, MA 01915 } david.audette-at-sylvania.com } } } } __________________________________________________ } Do You Yahoo!? } Thousands of Stores. Millions of Products. All in one Place. } http://shopping.yahoo.com/ }
** Anthony J. Garratt-Reed ** MIT Room # 13-1027 ** 77 Massachusetts Avenue ** Cambridge, MA 02139-4307 ** USA ** ** Phone: (+) 1-617-253-4622 ** Fax: (+) 1-617-258-6478 **
We are QS9000 certified which is the automotive equivalent to ISO. Basically, it really is up to you how often you want to do it. We have some instruments set up for twice a year and some on an annual basis. Basically, the ISO is a paper work tracking system that insures that you do what you say you do in your SOP's or in our case, SLP's (System Level Procedures). You decide what you want to do and stick to it. You write the specifications that you need to follow and document them. A suggestion, don't write them too stringent. You can use the ISO process to insure that your instrumentation is maintained properly. For example if you write a specification that your EDS detector should have a particular energy resolution for an element and if it is out, that the detector is repaired, then your company has no options, it must have it repaired or have it taken out of service. However, you should be careful in writing your procedures if you don't want it out of service. For example, you don't want the normal resolution degradation in performance with lifetime of a detector shutting you down.
We have the manufacturer's or our 3rd party's service engineers verify the calibration as part of the annual or semi-annual PM visit on our service contracts on all our scanning instruments. On our TEM, I just do the annual calibration verification myself and record it. If I do anything that requires a new calibration before the year is up, I just put the new calibration data in the records book. It doesn't hurt to do it more often. It is a real pain if you find out that it was out of calibration and you were sending data to customers. Then you have to contact them all and tell them that their results were done while it was not in calibration.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
} -----Original Message----- } From: Jesse Rodrigues [mailto:Jesse_Rodrigues-at-kopin.com] } Sent: Wednesday, November 08, 2000 4:02 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: ISO calibration of an SEM } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html } } } -------------------------------------------------------------- } ---------. } } } } } Hi, } } Does anyone have any experience with instituting ISO } compliance of SEM's? } How do you keep your SEM calibrated and how often are calibrations } performed? What type of support does your mfg. offer? } } Any information would be helpful. } } Thank you, } } Jesse Rodrigues } Kopin Corporation } 695 Myles Standish Blvd. } Taunton, MA 02780 } } }
Dear Listers, A test I have heard of for the acoustic resonence frequency of your TEM sample holder and stage is the "whistle test". While looking at a sample with good high res. detail through the binocular eyepieces, mag. 100,000 to 200,000 X, whistle starting low and going up. You should see a frequency in there where the sample vibrates. Never move or speak while taking a high res. photo. At 01:01 PM 11/8/00 -0500, you wrote: } Dear Gordon, } } Noise is a problem of inches. } } And Hertz. Our HVEM column is very sensitive to 20 Hz, but almost } completely insensitive to frequencies a few Hz away on either side. Once you } have someone sit at the scope and watch the image as a sound generator is swept } through a range of frequencies, you will know what to be concerned about, and } then you can get the appropriate shielding. } } The best solution is a corn field 5 miles } from any where in a 10 foot basment on a still night and a active noise } canceler makes it better. } } If you build it, will they come? ;-) } } We put our insterments in buildings with people } and macinery. I was testing a simple load cell with a 12 bid A/D } converter. It was no trick to measure a car drive by 30 yards away a truck } 100 yards a way a fellow walking by in the hall. And a load cell with a 12 } bit a/d converter is not a very sensitive insterment. } } There are good solutions down to 4 or 5 Hz below that you have trouble. } Floating it on an air table gets x and y but not z. floating it in liqid } work until you get a resonate situation. Active cancelation works the best } and costs the most. The things tried on this list sows that it is a } difficult problem. } } There is no free lunch. Floating the instrument renders it sensitive to } sound in the room, and mounting it solidly to the floor makes transmission } through the ground a problem. If the soil mechanics are such that the } frequencies transmitted to the instrument are not those to which it is } sensitive, but the sound emitted by the pumps is at the sensitive frequencies, } it can be better to bolt the instrument to the floor. As you said, an } environment completely free of vibration is best, and active cancellation is } good. } Yours, } } Bill Tivol } Wadsworth Center } Albany NY } (518) 473-7399 WFT02-at-health.state.ny.us } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
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The Microanalysis Research and Analytical Microscopy Groups at NIST have about twenty full time scientists specializing in the measurement methods in our extensive facilities including SEMs (FEG, EPMA, Environmental,=85), AEMs (FEG/LaB6 TEM/STEM/EDS/EELS), Auger probes, SIMS/TOF-SIMS, MicroXRD/XRF, MicroRaman/IR, Synchrotron beam-line with grazing incidence XPS, etc. We research new and improved measurement methods and we apply these methods to challenging analytical problems in semiconductor and optoelectronic technology, materials science, environmental and earth science, etc. {br} {br} If you have research ideas that would be related to the analytical approaches below and are looking for a Post Doc opportunity, please contact me. {br} {br} The NIST/NRC program offers a two-year Post Doc at an annual salary of $50,000 with an additional $5,500 for research expenses. The applications are {b} due to the NRC by Jan 15, 2001 {/b} . This includes a brief proposal and several recommendations. {br} {br} {b} A candidate must be a U.S. citizen and start work (with their PhD in hand) at NIST between July 1, 2001 and Feb 1, 2002. {/b} So, this is the perfect opportunity for those that are graduating this spring through next fall and others that have received their degree within the last five years. Please note that NIST/NRC only competes Post Doc positions once a year, unlike some other institutions. {br} {br} {b} General NIST-NRC Post Doc Information {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/vwLabInformation/03AF8E43C706150B8= 52567FB004AB8E8?OpenDocument"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/vwLabInformation/03AF8E= 43C706150B852567FB004AB8E8?OpenDocument {/a} {br} {br} {/font} {/u} Specific areas of interest: {br} {br} {b} Chemical Imaging {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/5273986D8FFE74B18525691F00= 60BD9F?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/5273986D8FFE74B= 18525691F0060BD9F?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Field Emission Analytical Electron Microscopy/Nanoscale Compositional Mapping {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/65DE73654E7869028525691F00= 60A463?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/65DE73654E78690= 28525691F0060A463?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Electron-Probe Microanalysis/Scanning Electron Microscopy {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/F704FABB051A2EE48525691F00= 60A4B5?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/F704FABB051A2EE= 48525691F0060A4B5?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Microchemistry in the Environmental Scanning Electron Microscope {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/944FC47A85E417348525691F00= 60C301?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/944FC47A85E4173= 48525691F0060C301?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Microbeam Mass Spectrometry {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/623A17BC37343CDC8525691F00= 60A44D?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/623A17BC37343CD= C8525691F0060A44D?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Nanoscale Crystallographic Analysis and Compound Identification by Diffraction Methods {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/39166A04610797758525691F00= 60C316?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/39166A046107977= 58525691F0060C316?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Optical Probe Microanalysis (Raman, IR Microprobes, and NSOM) {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/78E3849178AC6C718525691F00= 60A43A?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/78E3849178AC6C7= 18525691F0060A43A?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Submicroscopic Chemical and Physical Characterization of Materials and Particles {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/9C8BD7F79A5AAACE8525691F00= 60A4A1?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/9C8BD7F79A5AAAC= E8525691F0060A4A1?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Surface Microanalysis by Combined Auger Electron and X-Ray Emission Spectroscopies {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/9ACCD2318EE9230F8525691F00= 60B7E6?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/9ACCD2318EE9230= F8525691F0060B7E6?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} X-Ray Investigations of Thin Films, Surfaces, and Interfaces {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/362CD137E15600568525691F00= 60A4D5?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/362CD137E156005= 68525691F0060A4D5?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Instrument Performance Standards for Raman Spectroscopy {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/5E60A8DDDF3EB7DE8525691F00= 60BF12?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/5E60A8DDDF3EB7D= E8525691F0060BF12?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {b} Quantitative Surface Analysis by Electron=20 Spectroscopy {br} {/b} {font size=3D1 color=3D"#0000FF"} {u} {a= href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/D0188B23B4CC87B78525691F00= 60BD89?OpenDocument&50~NIST"= eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/D0188B23B4CC87B= 78525691F0060BD89?OpenDocument&50~NIST {/a} {br} {br} {/font} {/u} {br} {br} {div} Eric B. Steel, Leader &nbs= p; {x-tab} {/x-tab} e-mail:=20 eric.steel-at-nist.gov {/div} {div} Microanalysis Research Group {x-tab} {/x-tab} Office: 301-975-3902 {/div} {div} N.I.S.T. &nb= sp; &= nbsp; {x-tab} {/x-tab} {x-tab} = {/x-tab} FAX: 301-417-1321 {/div} {div} 100 Bureau Drive, Stop 8371 {/div} {div} Gaithersburg, MD 20899-8371 {/div} {a href=3D"http://www.nist.gov/cstl/div837/837.02/"= EUDORA=3DAUTOURL} http://www.nist.gov/cstl/div837/837.02/ {/a} {/html}
I seem you went to reflective mode of work. Sometimes it happens when charge does not flow from sample for some reasons. In this case you can see in backscattered electrons the image of the detector at polepiece (and some halves or quadrants will change contrast depending on what mode you choose), and in secondary electrons - all specimen chamber from inside. This effect can be used for example for testing of BSE signal path.
Advises: 1. Test the electrical path between the sample and microscope case (if you have absorbed current amplifier or meter the resistance may be large). 2. Check this effect on metal test sample. 2. Sputter non-metal sample by conductive film. 3. Work in low vacuum mode if you have it.
Hope it helps. Regards
Victor Sidorenko, ANTRON Co. Ltd., Moscow, Russia.
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That is a good question and I'm sure, one which will generate a high degree of variability of responses.
My (un-named) FESEM company does calibrations based on when the preventive maintenance (PM) routines are performed. These are supposed to be done at six month intervals. Actually, they get done only if I have a hard failure of the system and then when that problem is fixed, a PM is done....if there is time to do it.
In reality, I can do the key measurements myself to ensure that the SEM is in spec. Gun brightness, image symmetry, mag accuracy, etc.
I suppose that it depends on how much you want or need the maker to do the calibration versus what you can do or are allowed to do yourself.
gg
At 01:01 PM 11/8/00, you wrote:
} Hi, } } Does anyone have any experience with instituting ISO compliance of SEM's? } How do you keep your SEM calibrated and how often are calibrations } performed? What type of support does your mfg. offer? } } Any information would be helpful. } } Thank you, } } Jesse Rodrigues } Kopin Corporation } 695 Myles Standish Blvd. } Taunton, MA 02780
from: "Anthony Garratt-Reed" {tonygr-at-mit.edu} } Despite what many respondants to this message have said/implied, it is } entirely possible that Dave's IT department does know what it is talking } about. A Department/Plant network need not be implemented using TCP/IP, } and if it is all PC-based, and doesn't require routing, then NetBEUI might } be a sensible choice. IPX/SPX (Novell) networking, or even Lantastic or } another proprietary system may alternatively be in use.
Then let them build a gateway to put the Unix box on what ever they have. Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
} From: "William F. Tivol" {wft03-at-health.state.ny.us} , } Dear Bill,
} Noise is a problem of inches.
How true and the solution is often found by Edisonian iteration. } } And Hertz. Our HVEM column is very sensitive to 20 Hz, but almost } completely insensitive to frequencies a few Hz away on either side. Once you } have someone sit at the scope and watch the image as a sound generator is swept } through a range of frequencies, you will know what to be concerned about, and } then you can get the appropriate shielding. ======== Have you tried to find what is resonate at 20 Hz and do somting to break it up. That is high enough you might have a small chance of doing something about it. } } The best solution is a corn field 5 miles } from any where in a 10 foot basement on a still night and a active noise } canceler makes it better. } } If you build it, will they come? ;-) } We really do a lot of radio frequency noise testing in remote fields. Motorola has a 3 million dollar RF test chamber to test and certify some wireless devices for noise output on the receiving frequency. The company that owns the net work has a particular cornfield in Iowa they use for the same tests for considerably less money. John Deere tests the computers on their tractors for noise output and immunity by driving them out in the middle of a field using battery operated test instruments.
I built some sensors used on Ag machinery. It is the quietest environment I have ever seen The only noise was from the alternator on the diesel engine and the computers. NO 60 or 120 CPS noise at all.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
} from: "Anthony Garratt-Reed" {tonygr-at-mit.edu} } Despite what many respondants to this message have said/implied, it is } entirely possible that Dave's IT department does know what it is talking } about. A Department/Plant network need not be implemented using TCP/IP, } and if it is all PC-based, and doesn't require routing, then NetBEUI } might be a sensible choice. IPX/SPX (Novell) networking, or even } Lantastic or } another proprietary system may alternatively be in use. } } Then let them build a gateway to put the Unix box on what ever they have. } Gordon } Gordon Couger gcouger-at-couger.com } Stillwater, OK www.couger.com/gcouger } 405 624-2855 GMT -6:00
I guess that I just don't see what the big deal is here. Get a static IP, agreed-upon host name and set the Unix box up.
If the system is on a LAN, I don't see why routing is an issue. That will/should be transparent as long as the main router is set at the default gateway. If a bridge is involved, I can see that things might be more complicated--but not insurmountable.
Unix/Linux to PCs and Macs are done all the time. No big deal. My own LAN is exactly like this. Mac G4/400, 3 P-3/800, SGI O2, SGI Indigo 2, Sun Ultra 5, P2-400 Linux. All on 10BaseT running TCP/IP. PCs use Microsoft TCP/IP client. Mac runs DAVE. SGI runs IRIX 6.5. Sun runs SunOS/Solaris.
I simply do not see the big deal here. The key is to get a static IP on the branch LAN, know the gateway IP, and provide DNS IPs. That ought to do it.
I am in need of a Monte Carlo electron scattering programme (with or without EDS) for SEM scattering simulations. Is there one for downloading for a PC, freeware or otherwise?
David Cockayne Department of Materials University of Oxford Parks Road Oxford OX1 3PH England
Union Carbide is the manufacturer of ERL 4206, which is a component of of the Spurr Kit. They have discontinued the production of ERL 4206, but they do supply ERL 4221 which does not have the low viscosity of the ERL 4206. We continue to have ERL 4206 and Spurr Kits in stock and we are confident that we will have an ideal substitute for this product very shortly.
John Arnott We continue --
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Mendes, Maria wrote:
} Hello I would like to subscribe to your service, please let me know if there } is any service charge. } Please pass on my problem to your subscribers. } I am a research lab that does a lot of hard tissue processing, I as well as } our EM departments have been using SPURR as our plastic of choice. I have } been recently told by my supplier (Marivac) that in six months one of the } components of SPURR will be discontinued. The component is ERL } (Vinylcyclohexene Dioxide). I would like to know if others are using SPURR } and if so have they encountered the same problem. Please forward your } information to mmendes-at-mtsinai.on.ca. } Thank-you in advance for any help offered. } } Maria
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I just visited with Lucille Giannuzzi at Univ. Central Florida where she taught me a few things about FIB. I did ask her about carbon. She told me that they have a water injector to help with carbon films such as resists on semiconductors. Based on my experiences with DLC films, I suspect that you will need to use that to get reactive etching going to cut through the DLC. You will probably need to cut the samples normally, but you will also will need to watch out for channeling affects. Again, I learned more about this from Lucille.
I'm not the expert here. You might consider contacting one.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Daniel L Flatoff [mailto:dflatoff-at-stu.madison.tec.wi.us] } Sent: Wednesday, November 08, 2000 3:32 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: TEM sample prep of steel } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html } } } -------------------------------------------------------------- } ---------. } } } I would like to say thanks to all for the information } provided on preparing TEM cross sections of DLC thin films } on steel. Also I would like to note that I do have access } a FIB tool at Madison Area Technical College, so anyone who } has any info on specialized techniques for the TEM prep of } DLC thin films using the FIB would greatly be appreciated. } Again, thanks to all } } } Daniel L. Flatoff } student } Madison Area Technical College } } } }
The other day while on a consulting job that entailed photographing a set of ceramic tiles in a range of colors, I discovered an interesting quirk of at least this Olympus C-2500L digital camera. I suspect that other consumer/prosumer cameras would suffer the same fate.
There were four tiles ranging from, say, very light purple to dark purple. We'd put them on a black velvet background. We had tremendous trouble getting a accurate color. The two light tiles would be too bright and washed-out, and the two dark tiles would be too dark and not quite right. For that matter, the black wasn't black.
I recalled an article I'd read about the custom chips in this Olympus camera, and how it was much more an active system than just a CCD pixel bucket. It tries to make a nice picture based on what it saw.
My hypothesis was to add other objects to the scene - sure enough, after adding a few other colorful objects at the margins of the frame, the appearance of the tiles changed dramatically for the better. Now they were a range. It's as if it needs a sacrificial white and black and other colors in order to produce a decent image.
Certainly this has implications for microscope use of today's digital cameras. I don't see a way to turn off this feature, at least on this camera.
Greetings, Does anyone know of software that will run a digitizing tablet for the macintosh? I am thinking of something like "sigmascan" which runs on the PC. We just need to measure lengths and angles, nothing to fancy.
Thanks for the help. Tobias Baskin -- _ ____ __ ____ Tobias I. Baskin / \ / / \ / \ \ 109 Tucker Hall / / / / \ \ \ Biological Sciences /_ / __ /__ \ \ \__ University of Missouri / / / \ \ \ Columbia, MO USA / / / \ \ \ 65211-7400 / / ___ / \ \__/ \ ____ voice: 573-882-0173 fax: 573-882-0123
Does anyone have references and recommendations(or caveats)for dehydration of tissue using tBGE? We routinely prep (testis and ovary) with PO through Epon812 and want something less toxic that still gives good ultrastructure.
==================================== Jennifer Palmer ARBL Colorado State Univ Ft. Collins, CO 80523-1683 , USA voice:(970)491-1770 fax:(970)491-3557
Why not just simply digitize the image and then use NIH Image to measure distances and angles, this will produce a nicely documented measurement as you'll have the image, and all the numerical values stored.
I do this all the time. Of course, it presumes that you have a digitized image to start with.
Nestor Your Friendly Neighborhood SysOp
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================================================================== Dr. Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov ================================================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ==================================================================
The box said "This program requires Win 95/98/NT or better..." So I bought a G3 Mac !
Does anyone know a good reference for the origin of the top-bottom effect? TIA. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
From root Thu Nov 9 15:52:53 2000 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id PAA15526 for dist-Microscopy; Thu, 9 Nov 2000 15:44:58 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id PAA15523 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Thu, 9 Nov 2000 15:44:28 -0600 (CST) Received: from scribe.cc.purdue.edu (scribe.cc.purdue.edu [128.210.11.6]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id PAA15516 for {microscopy-at-sparc5.microscopy.com} ; Thu, 9 Nov 2000 15:44:17 -0600 (CST) Received: from [128.210.121.38] by scribe.cc.purdue.edu for microscopy-at-MSA.microscopy.com; Thu, 9 Nov 2000 16:43:25 -0500
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Hi all, I am looking for a sample to calibrate magnifications of a TEM from 50,000X up. I have used catalase in the past. However the periodicity of catalase is ~8.7nm. That is too large for my present purposes as I am looking at structures in the 2-4nm range and want to reduce the margin of error in the measurements as much as possible. I would also like a sample that is easy to used by inexperienced microscopists in the hopes of encouraging more users to calibrate instrument magnification on a regular basis. It also needs to be relatively in expensive so the loss of a grid is not a catastrophy. I would like a standard that would have ~1.0nm lattice. I thought I had found the perfect sample in copper phthalocyanine which has a spacing in this range. Unfortunately the only source of a grid with this crystal has discontinued carrying it. I am back to square one. My options are to prepare my own grid but need advise on how to crystallize the copper phthalocyanine (available from Sigma in powder form) or find another crystalline lattice in this size range. I would appreciate suggestions for alternative crystals and/or methods for making the copper phthalocyanine crystals to use for this purpose. Thanks in advance. Debby
Debby Sherman Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: sherman-at-btny.purdue.edu 1057 Whistler Building West Lafayette, IN 47907-1057
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id CAA00984 for dist-Microscopy; Fri, 10 Nov 2000 02:04:46 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id CAA00981 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 10 Nov 2000 02:04:16 -0600 (CST) Received: from oxmail.ox.ac.uk (oxmail1.ox.ac.uk [129.67.1.1]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id CAA00974 for {microscopy-at-msa.microscopy.com} ; Fri, 10 Nov 2000 02:04:04 -0600 (CST) Received: from heraldgate1.oucs.ox.ac.uk ([163.1.2.49] helo=frontend1.herald.ox.ac.uk ident=exim) by oxmail.ox.ac.uk with esmtp (Exim 3.12 #3) id 13u99U-0006OA-00 for microscopy-at-msa.microscopy.com; Fri, 10 Nov 2000 08:03:12 +0000 Received: from doole.materials.ox.ac.uk ([163.1.65.184]) by frontend1.herald.ox.ac.uk with smtp (Exim 2.02 #1) id 13u99V-0000Uv-00 for microscopy-at-msa.microscopy.com; Fri, 10 Nov 2000 08:03:13 +0000
Hi Debbie,
What about crocidolite 0.9nm (020) and 0.45nm (021) spacings. It's a long time since I used it but it is still in catalogues and cheap (Agar Scientific at £7 sterling each and probably others).
Good luck, Ron
On 09 Nov 2000 16:43:30 -0500 Debby Sherman {sherman-at-btny.purdue.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi all, } I am looking for a sample to calibrate magnifications of a TEM from 50,000X up. I have used catalase in the past. However the periodicity of catalase is ~8.7nm. That is too large for my present purposes as I am looking at structures in the 2-4nm range and want to reduce the margin of error in the measurements as much as possible. I would also like a sample that is easy to used by inexperienced microscopists in the hopes of encouraging more users to calibrate instrument magnification on a regular basis. It also needs to be relatively in expensive so the loss of a grid is not a catastrophy. } I would like a standard that would have ~1.0nm lattice. I thought I had found the perfect sample in copper phthalocyanine which has a spacing in this range. Unfortunately the only source of a grid with this crystal has discontinued carrying it. I am back to square one. My options are to prepare my own grid but need advise on how to crystallize the copper phthalocyanine (available from Sigma in powder form) or find another crystalline lattice in this size range. } I would appreciate suggestions for alternative crystals and/or methods for making the copper phthalocyanine crystals to use for this purpose. } Thanks in advance. } Debby } } Debby Sherman Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: sherman-at-btny.purdue.edu } 1057 Whistler Building } West Lafayette, IN 47907-1057 } }
---------------------- Mr. R.C. Doole Department of Materials, University of Oxford. Parks Road, Oxford. OX1 3PH. UK. Phone +44 (0) 1865 273701 Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
I have routinely used an ethanol series straight into Epon (or its various replacement incarnations) for over 20 years now, with never any poor embedding issues. Truly dry EtOH is miscible with the epons, but requires throrough mixing and additional graded steps. For standard tissues blocks, try 3x in 100% EtOH, followed by 2:1 EtOH:epon, 1:1 EtOH:epon 1:2 EtOH:epon, and at least 2 more pure epon prior to embedding. The 3 EtOH:epon steps should be at least 45 min each (1 have used up to 1 hr for dense tissues) and the pure epon steps can be 30 to 60 min each. I can't speak to the t-BGE issue, but I developed a skin sensitivity to PO a long time ago, and worked out the EtOH method to avoid the PO (I originally used acetone for the dehydration, but had a problem maintaining dry acetone--molecular sieves work but they can introduce particulate contaminants). Hope this helps.
Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc.
On Thu, 9 Nov 2000 11:33:32 -0700 (Mountain Standard Time), Jennifer Palmer wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Does anyone have references and recommendations(or caveats)for } dehydration of tissue using tBGE? We routinely prep (testis and } ovary) with PO through Epon812 and want something less toxic that } still gives good ultrastructure. } } ==================================== } Jennifer Palmer } ARBL } Colorado State Univ } Ft. Collins, CO 80523-1683 , USA } voice:(970)491-1770 } fax:(970)491-3557 } } jpalmer-at-cvmbs.colostate.edu } ==================================== } }
_______________________________________________________ Say Bye to Slow Internet! http://www.home.com/xinbox/signup.html
I have discussed John McCaffrey's Mag-I-Cal sample at length in the past. You can contact South Bay Technology, Electron Microscopy Sciences, or SPI. I believe all of them have it described on their web sites. It will do exactly what you want it for and then some.
Try it you'll like it.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
} -----Original Message----- } From: Debby Sherman [mailto:sherman-at-btny.purdue.edu] } Sent: Thursday, November 09, 2000 4:44 PM } To: message to: MSA list } Subject: TEM calibration sample } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html } } } -------------------------------------------------------------- } ---------. } } } Hi all, } I am looking for a sample to calibrate magnifications of a } TEM from 50,000X up. I have used catalase in the past. } However the periodicity of catalase is ~8.7nm. That is too } large for my present purposes as I am looking at structures } in the 2-4nm range and want to reduce the margin of error in } the measurements as much as possible. I would also like a } sample that is easy to used by inexperienced microscopists in } the hopes of encouraging more users to calibrate instrument } magnification on a regular basis. It also needs to be } relatively in expensive so the loss of a grid is not a catastrophy. } I would like a standard that would have ~1.0nm lattice. } I thought I had found the perfect sample in copper } phthalocyanine which has a spacing in this range. } Unfortunately the only source of a grid with this crystal has } discontinued carrying it. I am back to square one. My } options are to prepare my own grid but need advise on how to } crystallize the copper phthalocyanine (available from Sigma } in powder form) or find another crystalline lattice in this } size range. } I would appreciate suggestions for alternative crystals } and/or methods for making the copper phthalocyanine crystals } to use for this purpose. } Thanks in advance. } Debby } } Debby Sherman Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } } Purdue University E-mail: } sherman-at-btny.purdue.edu } 1057 Whistler Building } West Lafayette, IN 47907-1057 } }
We have a Physical Electronics 570 used Auger/ESCA/SIMS surface analysis instrument that we must part with. We used this primarily as a scanning Auger, but also did XPS and some SIMS. This instrument is in working order and has been under a service contract until recently. All offers or inquiries are welcome.
} ... } I discovered an interesting quirk of at least this } Olympus C-2500L digital camera. I suspect that other } consumer/prosumer cameras would suffer the same fate. } } There were four tiles ranging from, say, very light purple } to dark purple. We'd put them on a black velvet background. } We had tremendous trouble getting a accurate color. ... } } ... } } My hypothesis was to add other objects to the scene - sure } enough, after adding a few other colorful objects at the } margins of the frame, the appearance of the tiles changed } dramatically for the better. ... } } Certainly this has implications for microscope use of } today's digital cameras. I don't see a way to turn } off this feature, at least on this camera.
Could the camera be mis-judging the "white balance"?? On my camera I can disable "automatic", and force "incandescent", "fluorescent", "daylight", etc.
cheerios, =shAf= :o) {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
I recently requested, on this site, advice on programmes for Monte Carlo simulations of electron scattering. I have been asked by Nestor to summarise them, to save others asking the same question (and clogging the site). Here is a summary of many replies I received. I have not accessed many of them, and pass them on without comment.
2 site allowing to do online simulations as well as the possibility of as well as the possibility of downloading the program for SEM (and TEM) scattering simulations. http://callisto.my.mtu.edu/soft/mc/ss_java.html http://callisto.my.mtu.edu/soft/mc/ (this address for downloading)
6 Try Cnet (www.cnet.com) and enter Monte Carlo into the "search" box. A page will come up with a list of downloadable programs divided into purchaseable programs and freeware. No idea whether any of them works as I haven't tried any. I'm just familiar with Cnet as a source of downloable software.
7 Try Electron Flight Simulator by Small World, (500)447-3340
Microscopists, A researcher here at the University of Alaska is interested in paying to have some thin sections made from her silicate glass samples. Are there any labs out there that will contract to do ion milling? She is not on the list so please reply to Jessica Faust, her E-mail address is faust-at-gi.alaska.edu Thanks once again. Kim DeRuyter
University of Pittsburgh Department of Materials Science and Engineering
Postdoctoral Research Position in Transmission Electron Microscopy
An additional vacancy for a POSTDOCTORAL POSITION has become available in the area of transmission electron microscopy of thin film data storage media as part of a collaborative research program between Professors J.M. Wiezorek and W.A. Soffa, Department of Materials Science and Engineering of the University of Pittsburgh, and the Seagate Technology. Candidates should hold a Ph.D. in Materials Science, Physics or a related discipline and must have extensive hands-on experience in a broad range of imaging, diffraction and analytical transmission electron microscopy characterization techniques, as well as with sample preparation methods. Demonstrated expertise in the preparation of thin film samples suitable for high-resolution TEM characterization in plan-view and cross-section is highly desirable. A basic knowledge of magnetism in materials and experience in instrument development and/or computer image processing/simulation would be beneficial. The appointment is for one year in the first instance and is available from December 2000. Applications from under-represented groups, including minorities, women and people with disabilities are encouraged.
Interested candidates should send a curriculum vitae, including publication list, and the names of three referees with postal addresses, telephone numbers and E-mail addresses to: Prof. J.M. Wiezorek, Department of Materials Science and Engineering, University of Pittsburgh, 848 Benedum Hall, Pittsburgh, PA 15261, USA. Email: wiezorek+-at-pitt.edu
The Microcharacterization laboratory in the Department of Materials Science and Engineering of the University of Pittsburgh has an opening for a full-time technician in a multiple user facility. The facility's main instrumentation consists of two Xpert XRD, an XL30FEGSEM with EDS, BSE and EBSD, and two JEOL TEM's. The successful applicant will have a minimum of a Bachelor¹s degree and 3 years experience working with scanning electron microscopes and X-ray diffraction instruments. Responsibilities will include daily operation and maintenance of the SEM and XRD instrumentation including the training of users (including students), specimen preparation and documentation of experimental data. The successful applicant must demonstrate the necessary organizational, management and communication skills to efficiently operate in an academic multi-user environment. Applicants should submit a cover letter describing their experience with different instrumentation, a resume and contact information for three references. Salary will be commensurate with the candidate¹s experience.
Resumes should be sent to; Professor G. H. Meier, Interim Chair Department of Materials Science and Engineering 848 Benedum Hall University of Pittsburgh Pittsburgh, PA 15261
The University of Pittsburgh is an equal opportunity and affirmative action employer.
At 07:24 AM 11/10/00 -0800, michael shaffer wrote: } Could the camera be mis-judging the "white balance"?? } On my camera I can disable "automatic", and force } "incandescent", "fluorescent", "daylight", etc.
No, I'd already preset and locked the color temperature against a white card in the same environment. These other color adjustments were beyond simple white balance problems.
I know that this has been posted before but I lost the link. Does anyone have a recommendation for WEB scheduling software. Thanks. ______________________________ Roberto Garcia NCSU/Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh NC 27695-7531 P: (919) 515-8628 F: (919) 515-6965 rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif http://spm.aif.ncsu.edu/asm
This might be of use to those of you wanting to convert your older stereo and compound microscopes to digital imaging capability. We recently explored the possibilities of mounting a Nikon CoolPix 990 (CCD=3.34 million pixels) to our 14 year old Zeiss stereoscope and Standard microscopes. Neither has a "C" mount, which is what the Nikon adapter fits onto. There were several very expensive (} $1,500) possible solutions, but the most satisfying was one proposed by Spectra Tech (716-265-4320; e-mail Michael Specht {mspecht-at-frontiernet.net} ). For about $250, they were able to provide an adapter which screws directly into the camera and fits either in the ocular tube or phototube of the microscopes. Adapters can be purchased for different tube diameters. Contact them for further information if interested.
I have no financial interest.
Doug
---------------------- Douglas R. Keene Associate Investigator Shriners Hospital Research Facilities 3101 S.W. Sam Jackson Park Road Portland, Oregon 97201 phone: 503-221-3434 FAX: 503-412-6894 (9-5 PST) e-mail: DRK-at-shcc.org
Question: I am working on a final year project titled "low frequency surface microscopy". My project supervisor wants me to use a probe that consists of a concentric cylinder with a pin in between. This is to concentrate the field on the surface to be scanned. With the occurence of cracks, boundaries, etc. on the surface, the electric field and hence the capacitance is expected to change. The reflected voltage in the bridge circuit implementation gives a reflection of the surface characteristic.
My questions go thus (sorry for the preambles): 1. How do I design a probe that can help me to achieve this( what materials should be used, what is the expected capacitance value, what are the relevant equations) 2. At what frequencies can this function properly 3. Can you let me into the theory of this method and other similar methods? 4. Can you refer me to some links and/or send some reference materials to me?
At the moment, i am doing a physics assignment for my HSC assessment on the following topic:
"Compare the resolving powers of light and electrom microscopes and assess the impact of their development".
I you could help by sending some information, I would be very grateful. Thank you.
Sujitha
__________________________________________________ Do You Yahoo!? Thousands of Stores. Millions of Products. All in one Place. http://shopping.yahoo.com/
Doug, does this adapter have a relay lens? What zoom to you use and is there any vignetting of the field?
I'm buying adapters for a 990 right now.
Regards, Glen
On Fri, 10 Nov 2000, Douglas Keene wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } This might be of use to those of you wanting to convert } your older stereo and compound microscopes to digital } imaging capability. We recently explored the possibilities } of mounting a Nikon CoolPix 990 (CCD=3.34 million pixels) to } our 14 year old Zeiss stereoscope and Standard microscopes. } Neither has a "C" mount, which is what the Nikon adapter } fits onto. There were several very expensive (} $1,500) } possible solutions, but the most satisfying was one } proposed by Spectra Tech (716-265-4320; e-mail Michael } Specht {mspecht-at-frontiernet.net} ). For about $250, they } were able to provide an adapter which screws directly into } the camera and fits either in the ocular tube or phototube } of the microscopes. Adapters can be purchased for } different tube diameters. Contact them for further } information if interested. } } I have no financial interest. } } Doug } } ---------------------- } Douglas R. Keene } Associate Investigator } Shriners Hospital Research Facilities } 3101 S.W. Sam Jackson Park Road } Portland, Oregon 97201 } phone: 503-221-3434 } FAX: 503-412-6894 (9-5 PST) } e-mail: DRK-at-shcc.org } } } } } } } }
I have some epon-araldite blocks to section with tiny wallaby embryos embedded in. Unfortunately the blocks are very soft and the one micron sections are sticky and very hard to pick up does anyone have a solution please as I am getting very frustated Thanks in anticipation Joan Clark
Depending on the 'scope, it may have an intermediate lens/optics unit. I am using the CP 990 on Axioskop and Olympus and the common adapter unit has a lens.
gg
At 05:57 PM 11/12/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Can anyone advise what voltage and polarity should be applied to the BNC plug on the short co-axial cable labelled "OM1" which issues from the flange of the JEOL OM on an 840?
It's something to do with an electron deflector or collector.
tia
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
I have read here that you can put the blocks back in the oven at a higher temperature. Why not try this with an unwanted portion of a block?
Dave
On Sun, 12 Nov 2000 23:45:57 -0600 Joan Clark {j.clark-at-zoology.unimelb.edu.au} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have some epon-araldite blocks to section with tiny wallaby embryos } embedded in. Unfortunately the blocks are very soft and the one micron } sections are sticky and very hard to pick up does anyone have a solution } please as I am getting very frustated } Thanks in anticipation } Joan Clark } } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Try placing the blocks back in the oven at anything up to 100C for several hours, or even overnight. Remember that the blocks harden after removal from the oven for up to two hours; not just the couple of minutes it takes to physically cool. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Monday, November 13, 2000 3:46 PM, Joan Clark [SMTP:j.clark-at-zoology.unimelb.edu.au] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have some epon-araldite blocks to section with tiny wallaby embryos } embedded in. Unfortunately the blocks are very soft and the one micron } sections are sticky and very hard to pick up does anyone have a solution } please as I am getting very frustated } Thanks in anticipation } Joan Clark } }
} This might be of use to those of you wanting to convert } your older stereo and compound microscopes to digital } imaging capability. We recently explored the possibilities } of mounting a Nikon CoolPix 990 (CCD=3.34 million pixels) to } our 14 year old Zeiss stereoscope and Standard microscopes. } Neither has a "C" mount, which is what the Nikon adapter } fits onto. There were several very expensive (} $1,500) } possible solutions, but the most satisfying was one } proposed by Spectra Tech ... For about $250, they } were able to provide an adapter which screws directly into } the camera and fits either in the ocular tube or phototube } of the microscopes. Adapters can be purchased for } different tube diameters. ...
When you say your were "satisfied", what features/characteristics would you care to mention? Did the amount of field-of-view vary? How much of the field were you able to capture? (If satisfaction essentially boils down to price/quality, a similarly priced and quality eyepiece adapter is also available from Optem International {www.optemintl.com} ... however, I am testing their C-mount adapter).
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
I've been looking at some of these sites lately for web-based calendars. I don't necessarily recommend any of them. You'll see that each has shortcomings.
1. http://www.webevent.com (Can be run on all sorts of operating systems. Commercial software. Lower prices for non-profit institutions.)
2. http://srv.emunit.unsw.edu.au (Excellent, although it is probably only for Windows servers. Some accounting capabilities. Not commercial software. About $500 US.)
3. http://www.webprog.com/appoint/freetest.html (About $60 US. Requires a Windows server. Basic.)
4. http://cmmserv.mrl.uiuc.edu/calendar (UserNumber = 2399, password = DEMO, instrument = Zeiss DSM-960) (Windows server required.) } -----------------------------------------------------------------------. } } I know that this has been posted before but I lost the link. Does anyone } have a recommendation for WEB scheduling software. Thanks. } ______________________________ } Roberto Garcia } NCSU/Analytical Instrumentation Facility } Campus Box 7531 Room 318 EGRC } 1010 Main Campus Dr. } Raleigh NC 27695-7531 } P: (919) 515-8628 } F: (919) 515-6965 } rgarcia-at-unity.ncsu.edu } http://spm.aif.ncsu.edu/aif } http://spm.aif.ncsu.edu/asm
---------------------------------------------------------------------- Russell E. Cook, Ph.D. Electron Microscopy Center Argonne National Laboratory Materials Science Division, Building 212 9700 South Cass Avenue Argonne, IL 60439-4838 (630)252-7194 FAX: (630)252-4289 recook-at-anl.gov
We have been asked to embed some human skin biopsies for electron microscopy. We used our routine protocol for processing kidney biopsies which includes glutaraldehyde fixation, dehydration through ethanol and acetone and embeddment in PolyBed 812. Our protocol resulted in good embeddment all the layers of the skin except for the stratum corneum where there were numberous holes in the PolyBed. Is it possible to get good embeddment of this keratinized zone?
Thanks for any suggestion,
John
John M. Basgen Department of Pediatrics University of Minnesota Mayo Mail Code 491 420 Delaware Street SE Minneapolis, MN 55455 USA Phone: 612-625-7979 FAX: 612-626-2791 E-mail: basgen-at-tc.umn.edu
Does anyone have experience with the 990 under low light conditions? We normally shoot brightfield on our inverted metallograph, but there is the occasional need for darkfield and DIC.
Thanks in advance, Jim Hyres
-----Original Message----- } From: michael shaffer [mailto:epmalab-at-darkwing.uoregon.edu] Sent: Monday, November 13, 2000 10:40 AM To: DRK-at-shcc.org; Microscopy-at-sparc5.microscopy.com
Doug writes ...
} This might be of use to those of you wanting to convert } your older stereo and compound microscopes to digital } imaging capability. We recently explored the possibilities } of mounting a Nikon CoolPix 990 (CCD=3.34 million pixels) to } our 14 year old Zeiss stereoscope and Standard microscopes. } Neither has a "C" mount, which is what the Nikon adapter } fits onto. There were several very expensive (} $1,500) } possible solutions, but the most satisfying was one } proposed by Spectra Tech ... For about $250, they } were able to provide an adapter which screws directly into } the camera and fits either in the ocular tube or phototube } of the microscopes. Adapters can be purchased for } different tube diameters. ...
When you say your were "satisfied", what features/characteristics would you care to mention? Did the amount of field-of-view vary? How much of the field were you able to capture? (If satisfaction essentially boils down to price/quality, a similarly priced and quality eyepiece adapter is also available from Optem International {www.optemintl.com} ... however, I am testing their C-mount adapter).
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
I am looking for manuals for a Kevex 770 XRF. In particular we would be interested in copies of the circuit diagrams and documentation for the XRF unit and the Detector/Pulse Processor/ADC interface. The Kevex DELTA computer and software is not really of interest, since we would like to change it to a PC platform.
Also, any suggestions of other listservers, etc. to look would be appreciated.
Thanks in Advance, Jim Mabon
__________________________________________ James C. Mabon, Ph.D. Research Electron Microscopist Materials Research Laboratory University of Illinois at Urbana-Champaign 104 S. Goodwin Avenue Urbana, IL 61801 http://ntweb.mrl.uiuc.edu/cmm __________________________________________
OK, I'll bite. What the heck is color scanning EM, as referenced in the title of the following article?
TIA
Bob
Histochemistry of food tissue by colour scanning electron microscopy Mitsuhiko Yamada, Masako Nishimura, Takeo Suzuki, Shigeru Kawamata, Eisaku Oho, and Toshiaki Kimura, pp. 503-507. Journal of Electron Microscopy vol 49(3)
Robert R. Wise, Ph.D. Associate Professor of Plant Physiology Department of Biology and Microbiology University of Wisconsin Oshkosh Oshkosh, WI 54901 tele: (920) 424-3404 fax: (920) 424-1101 wise-at-uwosh.edu http://www.uwosh.edu/departments/biology/wise/wise.html
I am interested in finding an IGP for a Philips (T)EM410 that can be rebuilt / refurbished. Anybody got one lying around?
TIA Ron Veil
Veil Electron Instrument Lab Customer Services Tel: (209) 521-3332 FAX: (209) 521-3033 e-mail: veilcs-at-earthlink.net ________________________________________________________________ YOU'RE PAYING TOO MUCH FOR THE INTERNET! Juno now offers FREE Internet Access! Try it today - there's no risk! For your FREE software, visit: http://dl.www.juno.com/get/tagj.
I am interested in removing the cytoplasm from plant cells for viewing cell walls by SEM. the cells are epidermal cells of bean cotyledons, and I was wanting a simple and rapid method to get rid of the cytoplasm which doesn't harm the cell wall.
Also, I was wondering if anyone knew of an SEM image analysis program that can count things, do nearest-neighbour analysis, density measurements and so on. The things I want to measure are quite variable in shape and size, so it might be a little difficult.
Hi Mark, We use a commercially available washing powder called Ariel (Procter and Gamble) to remove the cytoplasm. Use a 5% (w/v) aqueous solution - it contains a Bacillus subtilis derived protease. References: Honegger, R. 1985. Scanning electron microscopy of the fungus-plant interface: a simple preparative technique. Trans Br. Mycol. Soc. 84: 530-533.
Mims et al. 1989. Ultrastructure of the haustorium of the peanut late leaf spot fungus Cerosporidium personatum. Can. J. Bot. 67: 1198-1202.
It worked quite well for us...good luck, Beth
} Hi everyone, } } I am interested in removing the cytoplasm from plant cells for viewing cell } walls by SEM. the cells are epidermal cells of bean cotyledons, and I was } wanting a simple and rapid method to get rid of the cytoplasm which doesn't } harm the cell wall.
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
hello, for life cell microscopy of fp-tagged cells we consider to buy a TILL PHOTONICS imaging system in combination with a Olympus microscope. We would appreciate any comments on this system or possible alternatives. Beside single channel recording we are also interested in multiple color imaging including FRET. We would like to hear comments on general system performance, usability, flexibility, support, and problems with the system.
} Does anyone have experience with the 990 under low light } conditions? We normally shoot brightfield on our } inverted metallograph, but there is the } occasional need for darkfield and DIC. } ...
With ISO senstivity selectable (100,200,400), and manual maximum aperture and a manual selectable shutter up to 8sec (including bulb), should allow anything you can "see" thru the eyepieces. But I do suspect an increase in noise as you approach the limits, and you might want a trinoc head which can throw 100% of the light at the camera.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
Electron Microscopy Facility at Fox Chase Cancer Center is seeking a motivated individual for a Technician/Research Assistant position.
Required qualifications: B.S. or M.S. in biology, 2+ years of experience in biological electron microscopy.
Responsibilities include sample preparation and electron microscopy (TEM/SEM), dark room work, computer image processing, report preparation. The great variability of work due to collaborations with large number of laboratories provides exceptional opportunity for professional growth.
We offer a competitive salary commensurate with experience, an excellent benefit package (health/dental insurance, pension plan, paid vacation) and a very friendly working environment.
For confidential consideration, please send a CV including a statement of experience to:
Dr. Michael Jarnik Fox Chase Cancer Center EM Facility 7701 Burholme Avenue Philadelphia, PA 19111 e-mail: m_jarnik-at-fccc.edu
We are looking for a replacement belt for the control unit of an LKB Ultrotome III 8800. Any ideas what-so-ever? It's only 25+ yrs old! Thanks in advance.
John Hardy City of Hope Medical Center Duarte, CA (626) 301-8265
If you have a mechanical/instrument shop at your institution, ask the gurus there for belt material. All you need to be careful of is matching the diameter of the new belt with the old one. I've done this in the past and it works really well. If there is no shop, go to a trusted hardware/parts store with the old belt and ask for a similar replacement.
Giant rubber bands do not work......(but it was worth a try!)
Tamara Howard CSHL
On Tue, 14 Nov 2000, John Hardy wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } We are looking for a replacement belt for the control unit of an LKB } Ultrotome III 8800. Any ideas what-so-ever? It's only 25+ yrs old! Thanks } in advance. } } John Hardy } City of Hope Medical Center } Duarte, CA } (626) 301-8265 } } jhardy-at-coh.org } } } }
We have a ten year old Panametrics Hyscan scanning acoustic microscope that we must part with. This includes an old computer, controller, scan drive, and tank. Does anyone have an interest in the system or part of it?
Salve, sono Emma Bonino, e le propongo un "sondaggio" e 7 iniziative online. Grazie e buona... azione!
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Salve, sono Emma Bonino, e le propongo un "sondaggio" e 7 iniziative online. Grazie e buona... azione!
Quali alleanze per le elezioni politiche ? "SONDAGGIO": http://www.radicali.it/action/sondaggio/
Azioni online:
ABORTO E CONTRACCEZIONE Per introdurre in Italia pillola abortiva RU486 http://www.radicali.it/action/ru486/
FREE SOFTWARE Petizione europea contro la brevettabilita' del software http://www.radicali.it/action/freesoft/
LEGALIZZAZIONE DROGHE Sostegno a Pannella e ai radicali sotto processo per disobbedienza civile http://www.radicali.it/action/legalize/
FIRMA DIGITALE E VOTO ELETTRONICO Richiesta al Governo per la legalita' delle elezioni politiche http://www.radicali.it/action/digital/
CONTRO LA TELEVISIONE DI STATO Per abolire la concessione unica radiotelevisiva http://www.radicali.it/action/rai/
CONTRO IL SINDACATO DI STATO Per l'abolizione del sistema di rinnovo automatico dell'iscrizione sindacale http://www.radicali.it/action/sindacato/
LIBERALIZZAZIONE TELECOMUNICAZIONI Per l'abolizione del canone Telecom http://www.radicali.it/action/telecom/
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We still have a Kevex 7000 system connected to a LSI11/23 computer. Now, we have problem with booting the computer when the analyser is connected to the PDP-bus. We think, but we don't know, if we ahve a problem with the Kevex Q23 board,which interfaces the analyser to the bus. However, do anybody have other suggestions? Or even better, is there a Q23 board out there?
Ypurs Sincererly Gunnar Kopstad
Vennlig Hilsen dr.ing Gunnar Kopstad overingeniør Avd f Patologi, Rit
Salve, sono Emma Bonino, e le propongo un "sondaggio" e 7 iniziative online. Grazie e buona... azione!
Quali alleanze per le elezioni politiche ? "SONDAGGIO": http://www.radicali.it/action/sondaggio/
Azioni online:
ABORTO E CONTRACCEZIONE Per introdurre in Italia pillola abortiva RU486 http://www.radicali.it/action/ru486/
FREE SOFTWARE Petizione europea contro la brevettabilita' del software http://www.radicali.it/action/freesoft/
LEGALIZZAZIONE DROGHE Sostegno a Pannella e ai radicali sotto processo per disobbedienza civile http://www.radicali.it/action/legalize/
FIRMA DIGITALE E VOTO ELETTRONICO Richiesta al Governo per la legalita' delle elezioni politiche http://www.radicali.it/action/digital/
CONTRO LA TELEVISIONE DI STATO Per abolire la concessione unica radiotelevisiva http://www.radicali.it/action/rai/
CONTRO IL SINDACATO DI STATO Per l'abolizione del sistema di rinnovo automatico dell'iscrizione sindacale http://www.radicali.it/action/sindacato/
LIBERALIZZAZIONE TELECOMUNICAZIONI Per l'abolizione del canone Telecom http://www.radicali.it/action/telecom/
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Carol, Thanks for the post. Since I want the grid for calibration, not resolution, I needed something with a lattice which was visible at magnifications from about 50,000X up. I may have found a couple of possiblilities. Esbestos fiber grids with 1.3nm periodicity are available commercially. Also Ted Pella has arranged to obtain Cu-phthalocyanine grids (which had been in their catalog but were discontinued) with a 1.0nm periodicity. I think that either or both of these should do the trick. Debby
Microscopical Optical Consulting (MOC) Inc. Helmut Patzig P.O Box 586 Valley Cottage, NY 10989 (914) 268-6450 MOCLeica-at-aol.com
They had picked up the Leica service and were able to help with my old LKB system.
Good Luck!
George R. Munzing Jr Strategic Technologies Group Engelhard Corporation 25 Middlesex/Essex Tpk. Iselin, NJ 08830 (732) 205-7030
"John Hardy" {jhardy-at-coh.org} on 11/14/2000 02:26:18 PM
To: "Microscopy" {Microscopy-at-sparc5.microscopy.com} cc:
We are looking for a replacement belt for the control unit of an LKB Ultrotome III 8800. Any ideas what-so-ever? It's only 25+ yrs old! Thanks in advance.
John Hardy City of Hope Medical Center Duarte, CA (626) 301-8265
MAS SPECIAL TOPICS WORKSHOP Dale Newbury and Ryna Marinenko
The NIST Microanalysis Research Group and the Microbeam Analysis Society will co-sponsor an MAS Special Topics Workshop, "Understanding the Accuracy Barrier in Quantitative Electron Probe Microanalysis and the Role of Standards," that will be held at NIST in Gaithersburg, Maryland October 15-18, 2001. This event is part of the 2001 celebration of the NIST Centennial. The limited attendance workshop will deal with the experimental and theoretical factors that currently limit the accuracy of quantitative results to approximately 2% relative. The topics will include sample and instrument parameters, counting statistics, correction procedures, individual matrix correction parameters, etc. In addition, one or more sessions will be specifically devoted to discussions on microanalysis standards. Invited speakers will be expected to provide a short (6 pages, maximum) manuscript of their paper at the time of the meeting. These manuscripts will be subsequently published in a monograph. The intent of the meeting and monograph is to provide detailed information on accuracy limits and on acceptable standards procedures in quantitative microprobe analysis that is presently not readily available in the literature.
Below is a proposed outline of presentations. Please feel free to suggest possible speakers (don't be afraid to call your own number!) and additional topics. We don't expect to be able to address all significant topics, but rather hope to initiate an ongoing dialogue that will be addressed by periodic special workshops on this topic.
We are now preparing a list of invited speakers, and we are actively soliciting volunteers who would like to be considered to present a talk, especially on microanalysis standards. Not all types or groups of microanalysis standards have been considered in this list below. We would like to cover as much of the periodic table as possible.
In addition, if you are simply interested in attending the meeting, please send your name, address, telephone number, email, and FAX to receive future information.
Factors that play a significant role in the 2% relative accuracy barrier … Experimental factors … Instrument stability: how good is it really? … Electron beam performance … WDS peak reproducibility … EDS performance … The role of the specimen … Preparation issues … Stability (beam damage) … Charging … Spectral processing … Peak deconvolution in WDS … Extracting intensities from EDS spectra (peak overlaps) … Quantitative matrix corrections … How well do matrix correction schemes work on data outside their native databases? … What is missing? Where is research needed? Who will do it?
Standards in Quantitative EPMA … Procedures for Preparation of Bulk Standards specimens for Quantitative EPMA … Sources, Characterization, and Validation of Standard Materials … Commercial, private, institutional sources … Composition, Microheterogeneity, Stability, etc. … Traceability, certification, intercomparisons, round robins … Industry/Technology Specific Standards … Mineralogy and Geology - sources of standard specimens and preparation procedures … Pure Element and Oxide Standards … Useful Stoichiometric Compounds - crystalline, amorphous, sintered … Sulphides, selenides, and tellurides … Rare Earth Elements … Halides … Alkali Earth Elements … Low-atomic number elements, C, N, B, Be … Glass standards
My last contact information for LKB belts and service is:
Norm Woodside for belts at about $50 apiece. Fax: 410-744-8522.
Pat Capogrosi for service. Telephone: 410-744-1580.
This information is over a year old, but worth a try. Also, the drive belts can be almost duplicated by belts that cost about $8-10 each, if you have a good machine or instrument repair shop with comprehensive belt catalogs. Here are some specs you can try:
Belt thickness: 0.5mm. Outer circumference: 18cm. Width: 5mm. Number of teeth: 39. Size of teeth: 1mm by 5mm. Height of teeth: 2mm. (Thanks to Preston Stogsdill for this information.)
I've never been able to find one that EXACTLY fits, but I've gotten close enough that the microtomes work fine, if you don't mind a little "bump" as you turn the knob to raise and lower the specimen arm. The problem is finding the exact number of teeth. The bump doesn't translate into choppy cuts, but you might feel it in the knob itself.
Hope this helps.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
Here is a protocol I developed over a few years of trying to examine stratum corneum (it was based of course on published protocols and suggestions from this listserve -- nothing novel). The only big differences between this and Michael Delannoy's reply are the extended dehydration and infiltration times, along with the use of ruthenium tetroxide. I found the latter to be a must if you are interested in preserving the intercellular lipids of the stratum corneum. Also I found the use of a dermatome led to increased splitting off of the outermost layers of corneocytes, so simply excising small pieces of skin was better. Hope this helps,
Karen Zaruba 3M Company, St. Paul, MN kszaruba1-at-mmm.com
Preparation of Skin for TEM examination of Stratum Corneum
At necropsy, excise the skin with a minimum of stretching or force. Pin out skin in containers of half-strength Karnovsky?s fixative, which is 2% glutaraldehyde + 2% paraformaldehyde in cacodylate Buffer (0.15M sodium cacodylate, pH 7.4, with 0.1 M sucrose). After about 1 hr. the outer tissue should be trimmed away. The central areas should be divided into final embedding sizes (1 x 1 x 3 mm) by cutting over a piece of dental wax using a Personna razor blade. Continue fixation for a total of 2-12 hr., then wash in buffer 3 x 5 min. Post-fix in 1% osmium tetroxide in buffer for 30 min. Wash in buffer without sucrose 2 x 5 min., then briefly in distilled water. Post-fix in 0.2% ruthenium tetroxide (with or w/o 0.25% potassium ferricyanide) in 0.1 M cacodylate buffer without sucrose for time indicated. Rinse in buffer or distilled water, 3 x 5 min. (Use buffer if being held overnight at this stage.) Note: RuO4 reacts violently with filter paper and alcohols. Also reacts with benzene rings and organics. Dehydrate in ethanol series, 25-35 min. each step from 30% through 95%, then 3 x 20 min. in 100%. Incubate in tert-Butyl Glycidyl Ether, 2 x 30 min. Infiltrate in a mixture of 2 parts t-BGE to 1 part Spurr?s resin for 1-2 hrs. Then follow with 1 part t-BGE to 2 parts Spurr?s resin overnight. Continue to infiltrate in Spurr?s resin alone, 3 changes over 6-8 hrs. Embed in fresh resin in flat molds, orienting to allow cross-sectioning of the epidermis. Polymerize at approximately 58 C for 1-3 days. To visualize lipid lamellae, do not post-stain. For best contrast of desmosomes and cellular detail, post-stain with standard Tannin/Uranyl Acetate stain followed by Reynold?s Lead Citrate.
----------------------------------------------------------------------- John Basgen wrote:
To those doing EM on skin biopsies:
We have been asked to embed some human skin biopsies for electron microscopy. We used our routine protocol for processing kidney biopsies which includes glutaraldehyde fixation, dehydration through ethanol and acetone and embeddment in PolyBed 812. Our protocol resulted in good embeddment all the layers of the skin except for the stratum corneum where there were numberous holes in the PolyBed. Is it possible to get good embeddment of this keratinized zone?
Thanks for any suggestion,
John
John M. Basgen Department of Pediatrics University of Minnesota Mayo Mail Code 491 420 Delaware Street SE Minneapolis, MN 55455 USA Phone: 612-625-7979 FAX: 612-626-2791 E-mail: basgen-at-tc.umn.edu
As part of a hazard review on our new ESEM, many questions were raised regarding the observation of biohazards. We won't be looking at actual human pathogens (although maybe others do?? CDC/NIH??). However, just looking at unfixed human skin puts us under the Class 2 category. Also we'll be observing non-pathogenic (opportunisitic) bacteria. Here are the questions:
Are there conditions inside the ESEM that would favor the creation of an aerosol from the sample? Assuming any of the sample becomes detached/airborn inside the chamber, would pumping down to higher vacuum prior to opening the door remove them (seems logical that it would)? How do you dispose of pump oil containing microbes/biohazards? Would anything capable of infecting humans even survive in pump oil? (Remember we're talking about viruses as well as bacteria here). Finally, would the chamber walls, etc. need to be decontaminated periodically?
If anyone has gone through this sort of safety review and generated a protocol, we'd love to benefit from your hard work!! Otherwise we'd appreciate any suggestions/opinions.
Thanks as always, Karen Zaruba 3M Company, St. Paul, MN kszaruba1-at-mmm.com
Hi- We are analyzing experimental charges of magnetite + ilmenite in a glass of rhyolitic composition. The problem we have run into is that the ilmenite grains are very small and thin, typicaly {10um in length, such that nearly all ilmenite analyses overlap the glass. My question is: Does anyone have any experience/know-how/ideas that would help in calculating the composition of the ilmenite from an analysis that is from a mixture of ilmenite + glass.
Analyses are with a JEOL 733 microprobe at 15kV and 5nA. We have not yet tried a lower kV that would potentially reduce the beam penitration depth. Our method of recalculation thus far has been to adjust the final analysis for density differences between glass and ilmenite following the method of Warren (LPSC ?1997). The fraction of glass is then subtracted from the analysis based on the amount of Sio2 (all SiO2 in the glass, no SiO2 in the ilmenite). We then adjust the result by noting that the ZAF correction applied to the original analysis is a function of the amount of glass overlap and extrapolate this relationship for each element in ilmenite back to a ZAF at 0% Sio2.
TEM EDS is a last resort as the sample would have to be mined out of a polished microprobe mount.
************************************************ ### Free the Psoas ### **************
Dr. Scott Kuehner kuehner-at-u.washington.edu Dept. of Geological Sciences ph.206-543-8393 Box 351310 Fax 206-616-6873 The University of Washington Seattle, Washington 98195-1310 ************************************************
On Wed, 15 Nov 2000 kszaruba1-at-mmm.com-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } } Hello fellow microscopists, } } As part of a hazard review on our new ESEM, many questions were raised regarding } the observation of biohazards. We won't be looking at actual human pathogens } (although maybe others do?? CDC/NIH??). However, just looking at unfixed human } skin puts us under the Class 2 category. Also we'll be observing non-pathogenic } (opportunisitic) bacteria. Here are the questions: } } Are there conditions inside the ESEM that would favor the creation of an } aerosol from the sample? } Assuming any of the sample becomes detached/airborn inside the chamber, would } pumping down to higher vacuum prior to opening the door remove them (seems } logical that it would)? } How do you dispose of pump oil containing microbes/biohazards? Would } anything capable of infecting humans even survive in pump oil? (Remember } we're talking about viruses as well as bacteria here). } Finally, would the chamber walls, etc. need to be decontaminated } periodically? } } If anyone has gone through this sort of safety review and generated a protocol, } we'd love to benefit from your hard work!! Otherwise we'd appreciate any } suggestions/opinions. } } Thanks as always, } Karen Zaruba } 3M Company, St. Paul, MN } kszaruba1-at-mmm.com } } } } }
We are in the market for buying a automated tissue processor for electron microscopy and a paper printer for the dark room. I will appreciate any recommendations for certain makes and models.
Does anyone know if there is a protein stain you can apply in vapour form in order not to change the structure of very delicat samples when using liquids (as for e.g. starch and fat)?
We've had an ESEM for about 7 years now, but we've never looked at anything remotely human in origin in it. However, I think I have a pretty good idea of what conditions are really like in the chamber of an ESEM, so I'll contribute my (fairly uneducated) guesses/impressions. Here goes:
} Are there conditions inside the ESEM that would favor the creation of an aerosol from the sample?
Don't think so - unless your sample was easily evaporated...
Assuming any of the sample becomes detached/airborn inside the chamber, would pumping down to higher vacuum prior to opening the door remove them (seems logical that it would)?
You're right....that does seem logical...
} How do you dispose of pump oil containing microbes/biohazards? Would } anything capable of infecting humans even survive in pump oil? (Remember } we're talking about viruses as well as bacteria here).
Being at an oceanographic institute, there are these really big blue vats out on the dock, put there to receive waste oil from our ships. When I change the oil in our rotary pumps, that's where it goes. I kind of doubt that any human pathogens could live in pump oil (but that's a real guess....)
} Finally, would the chamber walls, etc. need to be decontaminated } periodically? } Probably not, but if it was deemed necessary, I suppose a rinse with some kind of antiseptic wouldn't be a bad idea - as long as you kept the fluids away from the stage electrics :-)
Good luck with the new ESEM - they're pretty nice, well-built instruments.
Frank Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada
For safety reasons we fix human cell cultures and catheters before examination in our ESEM. Unfixed human and animal cells suffer beam damage in tungsten filament ESEMs so we do not feel we are missing out. It would be nice to look at the catheter biofilms unfixed but we plumped for a safety first approach.
Dave
On Wed, 15 Nov 2000 13:26:22 -0600 "kszaruba1-at-mmm.com"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } } Hello fellow microscopists, } } As part of a hazard review on our new ESEM, many questions were raised regarding } the observation of biohazards. We won't be looking at actual human pathogens } (although maybe others do?? CDC/NIH??). However, just looking at unfixed human } skin puts us under the Class 2 category. Also we'll be observing non-pathogenic } (opportunisitic) bacteria. Here are the questions: } } Are there conditions inside the ESEM that would favor the creation of an } aerosol from the sample? } Assuming any of the sample becomes detached/airborn inside the chamber, would } pumping down to higher vacuum prior to opening the door remove them (seems } logical that it would)? } How do you dispose of pump oil containing microbes/biohazards? Would } anything capable of infecting humans even survive in pump oil? (Remember } we're talking about viruses as well as bacteria here). } Finally, would the chamber walls, etc. need to be decontaminated } periodically? } } If anyone has gone through this sort of safety review and generated a protocol, } we'd love to benefit from your hard work!! Otherwise we'd appreciate any } suggestions/opinions. } } Thanks as always, } Karen Zaruba } 3M Company, St. Paul, MN } kszaruba1-at-mmm.com } } } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Microscopy folks, Anyone out there have any info on the Tracor-Northern 6100 diode array detector as used in the TN spectrophotometers? Feel free to point me to another list that is more appropriate for this question if you know of one. Thanks, Greg M. -- Gregory Mulhollan, Ph.D. Extreme Devices Inc. 101 West 6th Street Suite 200 Austin, TX 78701 (512)479-7740 x2231 voice (512)479-7750 fax mulhollan-at-extremedevices.com
} We are analyzing experimental charges of magnetite + } ilmenite in a glass of rhyolitic composition. } The problem we have run into is that the } ilmenite grains are very small ... My question is: } Does anyone have any experience/know-how/ideas } that would help in calculating the composition of the } ilmenite from an analysis that is from a mixture of } ilmenite + glass. } } ...
This will indeed be a problem ... not only do x-rays generated outside the ilmente reach the detectors, but they also travel through different compositions on their way to the spectrometers which are located differently relative to the ilmenites orientation in the glass. One spectro may measure Fe largely absorbed by the ilmenite, and another may measure Ti largely absorbed by glass. You would have to model each based on simplistic assumptions which may be unjustified.
cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu Geological Science's Electron Probe Facility - University of Oregon http://epmalab.uoregon.edu/
There is a position for an EM technician at the Electron Microscopy Core lab of the Biotechnology Program at the University of Florida in Gainesville. The laboratory is mostly a fee-for-service lab serving the needs of biological, biomedical and agricultural scientists at the university. Applicants must be skilled in the preparation of biological samples for both scanning and transmission electron microscopy. Experience with both PC and Mac as well as website management is desirable. Experience with digital imaging and fluorescence microscopy also a plus. This is a full time, permanent position with standard benefits of State of Florida employees.
For further details, reply to this message or contact Greg Erdos at the number below.
Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program PO Box 110580 University of Florida Gainesville, FL 32611
} Does anyone know if there is a protein stain you can apply in vapour form in } order not to change the structure of very delicat samples when using liquids } (as for e.g. starch and fat)?
} Thanks for your help. Gudrun
Dear Gudrun, I don't know what you intend to do, LM, EM, or other, but I once used a stain to detect peptides on chromatography paper (it was a long time ago). 1) Place the protein specimen in an enclosed box in a well-ventillated hood. 2) Mix equimolar solutions of HCl and KMnO4 (in the hood, of course), place the beaker containing the mix in the box alongside the specimen, and seal the box. The Cl-atoms generated will react with the peptide bonds to produce chloropeptides. 3) Add iodide (I added KI solution, but HI or ICl would probably work.). Instead of the last step, you could add anything which reacts with the chloropeptides and gives a signal which can be detected by whatever type of microscopy you want to use. This stain is extremely sensitive, because it can label each peptide bond, so a 100-residue protein can have 100 labels attached. Good luck; please let me know if this technique works for you. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
I don't have any specific experience with the analytes and the matrix that you reference, but have you tried using backscattered electron imaging? There's a difference of a little over six in the average atomic number of ilemite and magnetite with an even greater difference between the ilemite and the volcanic glass matrix, so atomic number contrast should be no problem. Most EDX systems offer routines for measuring phase area based on atomic number contrast which is fairly easily translated into volume concentration. You should have no problem with overlap using backscattered electrons as opposed to x-rays. In addition, with the help of an image analysis package, you can get size, shape, orientation and other information at the same time. Just a thought.
Bill
"S. Kuehner" {kuehner-at-u.washington.edu} on 11/15/2000 03:13:46 PM
To: "kszaruba1-at-mmm.com"-at-sparc5.microscopy.com cc: Microscopy-at-sparc5.microscopy.com (bcc: William H Roberts/US/FILM/DPT)
Hi- We are analyzing experimental charges of magnetite + ilmenite in a glass of rhyolitic composition. The problem we have run into is that the ilmenite grains are very small and thin, typicaly {10um in length, such that nearly all ilmenite analyses overlap the glass. My question is: Does anyone have any experience/know-how/ideas that would help in calculating the composition of the ilmenite from an analysis that is from a mixture of ilmenite + glass. Analyses are with a JEOL 733 microprobe at 15kV and 5nA. We have not yet tried a lower kV that would potentially reduce the beam penitration depth. Our method of recalculation thus far has been to adjust the final analysis for density differences between glass and ilmenite following the method of Warren (LPSC ?1997). The fraction of glass is then subtracted from the analysis based on the amount of Sio2 (all SiO2 in the glass, no SiO2 in the ilmenite). We then adjust the result by noting that the ZAF correction applied to the original analysis is a function of the amount of glass overlap and extrapolate this relationship for each element in ilmenite back to a ZAF at 0% Sio2. TEM EDS is a last resort as the sample would have to be mined out of a polished microprobe mount. ************************************************ ### Free the Psoas ### ************** Dr. Scott Kuehner kuehner-at-u.washington.edu Dept. of Geological Sciences ph.206-543-8393 Box 351310 Fax 206-616-6873 The University of Washington Seattle, Washington 98195-1310 ************************************************
This is slightly off the EM mark, but still in the ballpark for this List...
We recently received a donated xray diffractometer for which we need service and installation assistance. Does anyone know of a local rep or contact info??
It is a Philips APD 3720 Automated Power Diffraction System with a Philips XRG 3100 X-Ray Generator, vintage ca. mid-80's.
Thanks, all.
Ann Hein Lehman
---------------- Ann Hein Lehman EM Facility Manager Trinity College Hartford, CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman
I realize that you are pretty far away, but we offer an electron microscopy course here at USU, each spring semester, which includes a large segment on SEM.
William McManus Supervisor Electron Microscopy Facility Department of Biology Utah State University Logan UT 84322-5305
billEMac-at-cc.usu.edu 435-797-1920
-----Original Message----- } From: O. O. Ilori [mailto:sojilori-at-oauife.edu.ng] Sent: Thursday, November 16, 2000 7:47 AM To: microscopy-at-sparc5.microscopy.com
Hello Guys, I am looking for a place,an institute or something where I can be trained to operate an SEM. Any suggestions? Thanks.
Mr. O. O. ILORI DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING OBAFEMI AWOLOWO UNIVERSITY, ILE-IFE, OSUN STATE NIGERIA.
I don't have any specific experience with the analytes and the matrix that you reference, but have you tried using backscattered electron imaging? There's a difference of a little over eleven in the average atomic number of ilmenite and magnetite with an even greater difference between the ilmenite and the volcanic glass matrix, so atomic number contrast should be no problem. Most EDX systems offer routines for measuring phase area based on atomic number contrast which is fairly easily translated into volume concentration. You should have no problem with overlap using backscattered electrons as opposed to x-rays. In addition, with the help of an image analysis package, you can get size, shape, orientation and other information at the same time. Just a thought.
Bill
"S. Kuehner" {kuehner-at-u.washington.edu} on 11/15/2000 03:13:46 PM
To: "kszaruba1-at-mmm.com"-at-sparc5.microscopy.com cc: Microscopy-at-sparc5.microscopy.com (bcc: William H Roberts/US/FILM/DPT)
Hi- We are analyzing experimental charges of magnetite + ilmenite in a glass of rhyolitic composition. The problem we have run into is that the ilmenite grains are very small and thin, typicaly {10um in length, such that nearly all ilmenite analyses overlap the glass. My question is: Does anyone have any experience/know-how/ideas that would help in calculating the composition of the ilmenite from an analysis that is from a mixture of ilmenite + glass. Analyses are with a JEOL 733 microprobe at 15kV and 5nA. We have not yet tried a lower kV that would potentially reduce the beam penitration depth. Our method of recalculation thus far has been to adjust the final analysis for density differences between glass and ilmenite following the method of Warren (LPSC ?1997). The fraction of glass is then subtracted from the analysis based on the amount of Sio2 (all SiO2 in the glass, no SiO2 in the ilmenite). We then adjust the result by noting that the ZAF correction applied to the original analysis is a function of the amount of glass overlap and extrapolate this relationship for each element in ilmenite back to a ZAF at 0% Sio2. TEM EDS is a last resort as the sample would have to be mined out of a polished microprobe mount. ************************************************ ### Free the Psoas ### ************** Dr. Scott Kuehner kuehner-at-u.washington.edu Dept. of Geological Sciences ph.206-543-8393 Box 351310 Fax 206-616-6873 The University of Washington Seattle, Washington 98195-1310 ************************************************
Does anyone know of a way to stain for collagen in Epon embedded cardiac muscle for viewing by light microscopy?
Thanks for any suggestions.
Dotty
Dotty Sorenson Microscopy and Image Analysis Laboratory Department of Cell and Developmental Biology University of Michigan Medical School Ann Arbor, Michigan dsoren-at-umich.edu (734)763-1170
Of course immunolabeling might work. I've had success with collagen types III and IV in Epon and Spurr's resin, definitely not processed with immuno in mind.
On the other hand if you just want something simple to help highlight the collagen a little, I've seen Methyl Green staining collagen more than other components in skin cross sections.
Good luck, Karen
--------------------------------------- Hello all,
Does anyone know of a way to stain for collagen in Epon embedded cardiac muscle for viewing by light microscopy?
Thanks for any suggestions.
Dotty
Dotty Sorenson Microscopy and Image Analysis Laboratory Department of Cell and Developmental Biology University of Michigan Medical School Ann Arbor, Michigan dsoren-at-umich.edu (734)763-1170
Come join us at the joint meetings of Florida Microscopy Society, FL AVS, and Surface Science 2001
March 12-16, 2001
University of Central Florida, Orlando, FL
THERE IS NO REGISTRATION FEE TO ATTEND THE MEETING (but please register at http://natasha.eng.usf.edu/gilbert/avs/anouncement/registrationform.html so we get an accurate head count for meals)
- An FIB afternoon session followed by:
- An early evenning session devoted to an FIB workshop and users group meeting (open to all users of FIBs and all FIB manufacturers)
- There will be 2 FIB-related short courses one on theory (see www.vacuum.org for fees and information) FIB will be included in a comprehensive TEM specimen preparation course March 14-16, 2001 (contact lag-at-mail.ucf.edu)
To submit an abstract for the meeting or for more information follow the FL local affiliates link to FL from: www.microscopy.org
or contact Lucille Giannuzzi, lag-at-mail.ucf.edu
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Mechanical, Materials, and Aerospace Engineering Director, UCF/Cirent Materials Characterization Facility University of Central Florida 12443 Research Parkway, Suite 305 Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
Does anyone have an experience that they would like to share involving the SVMicro digital camera by Sound Vision? I'm hoping to learn anything at all, from delivery, support, service, image quality, product reliability, etc. Thanks in advance.
We have one. It is about two years old. It can provide good images, but it is tough to work with and you have to work too hard to get a good image. The TWAIN software that I have is tough to set up the contrast, brightness, and exposure. The person to whom I gave this camera didn't like it, gave up and went back to film. I then traded him my Pixera camera for the SVMicro and he was much happier. I don't know if the software on the SVMicro has improved or not.
We don't do really critical work with either microscope that these are put on and we usually don't do color.
I would trade the SVMicro for another El Cheapo Pixera for the ease of use.
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
-----Original Message----- From: William H Roberts [mailto:William.H.Roberts-at-usa.dupont.com] Sent: Thursday, November 16, 2000 4:38 PM To: Microscopy-at-sparc5.microscopy.com Subject: SVMicro Digital Microscope Camera
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Does anyone have an experience that they would like to share involving the SVMicro digital camera by Sound Vision? I'm hoping to learn anything at all, from delivery, support, service, image quality, product reliability, etc. Thanks in advance.
We have a SVMicro attached to an old Zeiss Ultraphot. We do not do anything critical with it; just BF/DF and DIC at medium mags on materials samples. I would say it works quite well. We use the Photoshop interface for the Mac; the interface is quite easy to work with. One caveat, the sensor is quite large (~25mm) so you need good transfer optics to get a good image over the whole sensor. At the time (~2yrs bp), the performance to price ratio was very high. The whole package was around $1100. I do not know how it compares now. Just my $0.02.
Ray D. Twesten, PhD. Center for Microanalysis of Materials Seitz Materials Research Laboratory 104 S. Goodwin Ave., Urbana, IL 61801 +1 217 244-6177 (Fax -2278)
-----Original Message----- } From: Walck, Scott D. [mailto:walck-at-ppg.com] Sent: Thursday, November 16, 2000 5:07 PM To: 'William H Roberts' Cc: 'Microscopy'
We have one. It is about two years old. It can provide good images, but it is tough to work with and you have to work too hard to get a good image. The TWAIN software that I have is tough to set up the contrast, brightness, and exposure. The person to whom I gave this camera didn't like it, gave up and went back to film. I then traded him my Pixera camera for the SVMicro and he was much happier. I don't know if the software on the SVMicro has improved or not.
We don't do really critical work with either microscope that these are put on and we usually don't do color.
I would trade the SVMicro for another El Cheapo Pixera for the ease of use.
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
-----Original Message----- From: William H Roberts [mailto:William.H.Roberts-at-usa.dupont.com] Sent: Thursday, November 16, 2000 4:38 PM To: Microscopy-at-sparc5.microscopy.com Subject: SVMicro Digital Microscope Camera
--------------------------------------------------------------- --------- The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html
Does anyone have an experience that they would like to share involving the SVMicro digital camera by Sound Vision? I'm hoping to learn anything at all, from delivery, support, service, image quality, product reliability, etc. Thanks in advance.
Hi, We are looking for opinions or reviews on the the Casio QV 3000 digital camera, with a 340 MB microdrive and macro focusing down to 6 cm. any help would be greatly appreciated. thanks, Beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
I would appreciate comments and suggestions on automatic sample processors for EM. Vendors welcome. Marek Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories University of California, San Diego
Is there any other stain reagents, other than UA and LC, that are used in TEM? If so, do they have comparable results, are they ETOH or water based, what are there disposal precautions, and what are their staining protocols.
Any information will be appreciated.
Thanks in advance.
Donald G. Awbrey, HT(ASCP) QIHC Electron Microscopy / Image Analysis DonaldAwbrey-at-TexasHealth.org donaldawbrey-at-hotmail.com (817)-878-5647
I am in the process of publishing a brochure that advertises the AVS short courses and symposium in Florida. Since this is joint meeting would it be possible to rent/exchange a mailing list from the Society for this purpose. The meeting is March 2001.
Della
_____________________________
Della Miller AVS West 1265 El Camino Real, Ste. 109 Santa Clara CA 95050
I have walked into a laboratory that has had an Edwards E306A Vacuum Coater purchased in 1991. It has been used very rarely since, and by no one presently working in the laboratory. The instruction manuals help to pump down the system and get it ready for the application, but I have yet to get the carbon source deposited on the sample we are trying to coat for SEM analysis (I can get the carbon rods glowing, but nothing else happens).
I am wondering if anyone out there has this system and knows how to operate it with the carbon evaporation source. Any help to what I could be doing wrong, or not doing at all would be appreciated. Also perhaps if I could call someone who has this system and they could walk me though the steps would be great.
- Jonathan
(I have contacted the manufacturer and they have not made this coater in years so know little about it as well)
and do an ALL groups, all history search for "casio 3000." I did this and found 800 hits. Most are as expected in the rec.photo.digital usenet forum. I did not immediately see any threads regarding its use on a microscope. So, this may or may not be of value to you.
gary g.
At 07:09 AM 11/17/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
At 10:27 AM -0600 11/17/00, Awbrey, Donald wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Yes there are other TEM stains, although UrAc and Lead Citrate are by far the most commonly used. You can also use Bismuth in lieu of lead. Kits are commercially available and come with instructions. Its a similar protocol to that used for Pb, but with concetration and time differences. For a comprehensive review, you can refer to Vol. 5 Part I: Staining Methods for Sectioned Material. by PR Lewis and DP Knight in the "Practical Methods on Electron Microscopy series edited by Audrey Glauert. This covers general as well as cytochemical methods.
Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
I came across a used Blazers Electon Beam Evaroration unit with a 552 gun BAF 300 Freeze Etch unit BAE 300 Coating unit 300, Crystal thin film monitor QSG 202, dual rotary vane vacuum pumps and what appears to be all documentation. .
The unit was removed from a working lab but it was not decommissioned by experienced professionals. It did appear to be carefully taken down. I think it was taken for a decommissioned lab because many their notes on procedures are included
It is for sale by a friend of mine who I won't plug on the list. But it looks like it might be a good deal for some one that need a set up like this and he is not very proud of it. I don't normally do this but I hate to see something that appears to be this good go for scrap. Even though I would like to have the vacuum chamber.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
From root Fri Nov 17 20:59:40 2000 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id UAA16208 for dist-Microscopy; Fri, 17 Nov 2000 20:42:07 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with SMTP id UAA16205 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 17 Nov 2000 20:41:36 -0600 (CST) Received: from mail.eclipse.net (mail.eclipse.net [207.207.192.13]) by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) with ESMTP id UAA16198 for {Microscopy-at-sparc5.microscopy.com} ; Fri, 17 Nov 2000 20:41:25 -0600 (CST) Received: from chris.dev.null (nwt1-01-160.dial.nwt.eclipse.net [207.207.231.160]) by mail.eclipse.net (8.9.1a/8.9.1) with ESMTP id VAA26478 for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 17 Nov 2000 21:40:26 -0500 (EST) Message-Id: {5.0.1.4.2.20001117213926.01946c00-at-mail.eclipse.net} X-Sender: egon-at-mail.eclipse.net X-Mailer: QUALCOMM Windows Eudora Version 5.0.1
Good Evening, all:
We have an old LKB Ultramicrotome III (series 8800) that has developed a problem in the past few weeks. Apparently the cantilever arm is not going down far enough on the cutting stroke to activate the kickback on the knife holder, resulting in wet blocks nearly every stroke. Needless to say, this is a huge pain in the butt. We're kind of fond of the old machine, so we're interested in fixing it. My question is: does anyone know if there is an adjustment to change at what point the relay for the knife holder kicks in? Or, is there some way to get the arm to travel farther down? Thanks much!
-Chris
(Sorry....I don't have a fancy title yet...I'm still just a student!)
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Hi folks, Anyone out there have any info on the Tracor-Northern 6100 diode array detector as used in the TN spectrophotometers? Feel free to point me to another list that is more appropriate for this question if you know of one. Thanks, Greg M. -- Gregory Mulhollan, Ph.D. Extreme Devices Inc. 101 West 6th Street Suite 200 Austin, TX 78701 (512)479-7740 x2231 voice (512)479-7750 fax mulhollan-at-extremedevices.com
For microscopists in the state of Virginia. Please contact Lou Solebello at microls1297-at-mindspring.com if you are interested in getting involved, or would attend regular meetings held in Richmond. I would like to start up a Virginia Microscopical Society if there is enough interest to do so. Professionals, hobbyists, teachers all welcome. Let me know if you are interested, and what types of activities and presentations you would like to see. Lou Solebello
Dear listers, I've inherited an old (1970s?) Leitz Orthoplan microscope in great need of a cleaning and possibly some new objectives. Can anyone give advice on who can help me rehabilitate it? I'm in the Atlanta-Birmingham-Montgomery (Alabama) area. Thanks Missy Josephson
Eleanor M. Josephson, D.V.M., Ph.D. Assistant Professor Department of Anatomy, Physiology and Pharmacology College of Veterinary Medicine 111 Greene Hall Auburn University, AL 36849-5518 Ph. (334) 844-5423 FAX (334) 844-4542 josepem-at-vetmed.auburn.edu
} We have an old LKB Ultramicrotome III (series 8800) that has } developed a problem in the past few weeks. } My question is: does anyone know if there is an adjustment to change } at what point the relay for the knife holder kicks in?
Yes, there is and I have the relevant LKB service note somewhere. I'll be happy to fax it to you if you email me a fax number that will reach you.
Regards
Rob
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa **
Dear Sir/Madam , JEM3010 was manufactured in 1999.it has got 1.500.000x mag and digital camera system JSM5600 has got 300.000xmag and EDS system. we have two kind of chose for prices. If we study together (I mean we preapare a paper together), we reseach samples without any price. On the other hand we get approximately 30$ per each sample. But if you have many samples we can make a discount. Thanks for your interests.. I am waiting for your emails
********************************** ********************************** ** Research.Asst.ERDEM YASAR ** ** University of Kirikkale ** ** Department of Physics ** ** TEM LABORATORY ** ** 71450 KIRIKKALE/TURKEY ** ** erdem.yasar-at-physics.org ** ** yasar-at-science.ankara.edu.tr ** ** yasar-at-turkuaz.kku.edu.tr ** ********************************** **********************************
Hi Beth, I'm using the Casio QV 3000 privately and I'm very satisfied with it. High resolution, light sensitive CCD, easy to use functions and easily understandable menus. Nearly all my images are correctly exposed, and the macro setting works great, with really high resolution. It uses standard batteries, or as I do rechargeables, that can be bought everywhere. The camera body is slightly larger than most of the competitors, but this makes it so much easier to hold with a steady grip. All this together with the possibility of storing 244 images at high resolution with the 340 mB drive makes it one of the best choices. The Photoloader software package is easy to work with and makes the handling of your images in the PC fast and well arranged. I'm using a Lexar card reader, and image transfer to my PC is really fast, although USB usage is even faster. The only disadvantage I´ve found is that the software does not save images in TIFF- format, only JPEG. It's a good alternative to my Hasselblad and a lot easier to carry around outdoors. Now, you did not state what you need it for, ordinary photography is as you can see OK, but I think it would be hard to get adaptors to use it, for example, as a microscope camera .
Yours sincerely
Per Hörstedt Department of Medical Biosciences Pathology Unit for Electron Microscopy University of Umeå S-90187 Umeå Sweden
We have two Edwards 306 coaters in our lab. As with all carbon rod based evaporators you will need to experiment with the spring pressure holding the rods together. Too much pressure and the rods will glow and not arc properly (most likely the situation you described) or, too little pressure and the arc will cease prematurely. On the 306 coaters the spring pressure is adjusted by lengthening or shortening the carbon rods - first loosen the clamping collars and slide the rods through.
Hope this is some help.
David Vowles Electron Microscope Unit Dept of Materials Science and Metallurgy University of Cambridge Pembroke St Cambridge UK CB2 3QZ Tel: +44 1223 334325 Fax: +44 1223 334567 Email: djv23-at-msm.cam.ac.uk
On Fri, 17 Nov 2000, Dunlap, Jonathan C. wrote:
} I have walked into a laboratory that has had an Edwards E306A Vacuum Coater } purchased in 1991. It has been used very rarely since, and by no one } presently working in the laboratory. The instruction manuals help to pump } down the system and get it ready for the application, but I have yet to get } the carbon source deposited on the sample we are trying to coat for SEM } analysis (I can get the carbon rods glowing, but nothing else happens).
there are two possibilities that spring to mind: 1. you've probably checked this but if the cutting range is set too high the arm might not fall far enough to engage the retract mechanism. 2. something in the retract circuit may have blown. It happened once on an LKBI and the engineer found a blown capacitor. I assume that there must be a fairly big current activating the electromagnet.
Have you watched the cutting cycle through the binoculars? Does it appear to try to move at the bottom of the cutting stroke or make a clicking noise? How far below the mid knife position does the specimen arm travel (it should be several millimetres)?
Good luck
Malcolm Haswell e.m. unit University of Sunderland UK
christopher wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Good Evening, all: } } We have an old LKB Ultramicrotome III (series 8800) that has developed a } problem in the past few weeks. Apparently the cantilever arm is not going } down far enough on the cutting stroke to activate the kickback on the knife } holder, resulting in wet blocks nearly every stroke. Needless to say, this } is a huge pain in the butt. We're kind of fond of the old machine, so we're } interested in fixing it. } My question is: does anyone know if there is an adjustment to change at } what point the relay for the knife holder kicks in? Or, is there some way } to get the arm to travel farther down? } Thanks much! } } -Chris } } (Sorry....I don't have a fancy title yet...I'm still just a student!)
We used to use 1%KMNO4 in phosphate buffer, pH 6.5 instead of UA. We stained for 5min. It is supposed to be good for membranes. We flushed it down the sink.
Dave
On Fri, 17 Nov 2000 10:27:29 -0600 "Awbrey, Donald" {DonaldAwbrey-at-texashealth.org} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } } Dear TEM netters, } } Is there any other stain reagents, other than UA and LC, that are } used in TEM? If so, do they have comparable results, are they } ETOH or water based, what are there disposal precautions, and what } are their staining protocols. } } Any information will be appreciated. } } Thanks in advance. } } } } Donald G. Awbrey, HT(ASCP) QIHC } Electron Microscopy / Image Analysis } DonaldAwbrey-at-TexasHealth.org } donaldawbrey-at-hotmail.com } (817)-878-5647 } } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
I have responded to these queries before, so here goes again. The unit I have used for years is the Lynx Tissue Processor (currently available from EM Sciences). The original unit I purchased (from the manufacturer) was marvelous. A second unit (purchased when Leica was marketing it) took over 5 years to get it into reliable working condition. My guess is that EMS will provide a reliable product. The only other unit I know of is sold by RMC (recently cycled through Ventana and now Boekeler -- Tucson, AZ) that is an updated version of the old LKB design. I know of very happy users for this product, but I honestly do not know the current status under the new owners. I have attempted to get a demo of the RMC device, but RMC never followed through, and the last incarnations have occurred so quickly that I still can't provide a personal comparison.
Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. On Fri, 17 Nov 2000 00:19:10 -0800, Marek Malecki wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I would appreciate comments and suggestions on automatic sample processors } for EM. Vendors welcome. } Marek Malecki, M.D., Ph.D. } Director and Principal Investigator } } Molecular Imaging Laboratories } University of California, San Diego } } address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA92093-0368 } phone - office: 8588223373 } phone - cell: 8583443347 } fax: 8588223715 } e.mail: mmm-at-ucsd.edu } pager: 8586161420 } e.mail: mmm-at-ucsd.edu } e.pager: 1620024619-at-alphapage.airtouch.com } www site: http://mil.ucsd.edu/ } ftp site: mil1.ucsd.edu } } }
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In addition to the Glauert series, there are the Hayat books (one specifically on stains, and volume one of the EM series -- both may be out of print now, but they are all available through interlibrary loan) and any of the basic biological TEM books also should cover (at least mentioning) stains other than UrAc and Pb Citrate. Bismuth is the only one I have used, and it is really a bear to work with. Potassium permanganate has also been used as have lanthanum, phosphotungstic acid and ruthenium. The Hayat book is "Stains and Cytochemical Methods", Plenum Press, 1993. The other one is "Principles and Techniques of Electron Microscopy; Biological Appications, Volume I". Wiley has a huge volume on EM methods (it is a loose-leaf beast that comes with updates--or it used to) that includes all of these methods as well.
Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. On Fri, 17 Nov 2000 10:27:29 -0600, Awbrey, Donald wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } } Dear TEM netters, } } Is there any other stain reagents, other than UA and LC, that are } used in TEM? If so, do they have comparable results, are they } ETOH or water based, what are there disposal precautions, and what } are their staining protocols. } } Any information will be appreciated. } } Thanks in advance. } } } } Donald G. Awbrey, HT(ASCP) QIHC } Electron Microscopy / Image Analysis } DonaldAwbrey-at-TexasHealth.org } donaldawbrey-at-hotmail.com } (817)-878-5647 } } }
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Jonathan: Don't feel all alone. I purchased a 306A about 16 years ago when Edwards was manufacturing and marketing the units. Even then, they didn't seem to know all that much about the units. I had problems with the main valve, and got less than no help. I don't think I EVER got a decent coat of carbon out of the unit. I did manage to melt down a set of carbon rod holders and burned out a transformer, but it never did provide a decent carbon coat. It did provide a good metal (read, gold, for SEM) coat, and I had purchased it because of access to electrodes and the great rotary table provided with it. However, I eventually went back to the old reliable Denton for everything but the gold coating, and then managed to get a sputter coater, so the 306A was left standing, abandoned. Justice at last.
Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. On Fri, 17 Nov 2000 14:24:30 -0500, Dunlap, Jonathan C. wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello All: } } I have walked into a laboratory that has had an Edwards E306A Vacuum Coater } purchased in 1991. It has been used very rarely since, and by no one } presently working in the laboratory. The instruction manuals help to pump } down the system and get it ready for the application, but I have yet to get } the carbon source deposited on the sample we are trying to coat for SEM } analysis (I can get the carbon rods glowing, but nothing else happens). } } I am wondering if anyone out there has this system and knows how to operate } it with the carbon evaporation source. Any help to what I could be doing } wrong, or not doing at all would be appreciated. Also perhaps if I could } call someone who has this system and they could walk me though the steps } would be great. } } - Jonathan } } (I have contacted the manufacturer and they have not made this coater in } years so know little about it as well) }
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We are looking for antibodies to mitochondrial proteins , for a immunocytochemical study on the effect of parasites in the development of youngs birds . Does anyone or company have mitochondrial antibodies ?
Beth...I have owned the Casio QV-3000 since April 2000. I think it a totally awesome for Macro work. A picture is worth a 1000 words...please go to my online photo album and look for yourself. Some of the pictures have a reference scale in them.
http://communities.msn.com/CactusCritters
Best,
Al Coritz Sales & Service Manager RMC-Boeckeler Instruments 4650 S. Butterfield Dr. Tucson, AZ 85714 Voice: 520-745-0001 Cell: 520-465-3598 Fax: 520-745-0004 Email:Al-at-Boeckeler.com Website:RMCProducts.com
-----Original Message----- } From: Beth Richardson [mailto:beth-at-dogwood.botany.uga.edu] Sent: Friday, November 17, 2000 8:10 AM To: microscopy-at-sparc5.microscopy.com
Hi, We are looking for opinions or reviews on the the Casio QV 3000 digital camera, with a 340 MB microdrive and macro focusing down to 6 cm. any help would be greatly appreciated. thanks, Beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
I've used one to mitochondrial hsp70 from Affinity BioReagents that works well. Their number is: (800) 278-2424. The catalogue number for the antibody is MA3-028. In our hands it works in human and mouse cells....never tried it on birds or inverts.
The other option would be to use one of the MitoTracker dyes from Molecular probes.
No financial interest in either company, unfortunately!
Tamara Howard CSHL
On Mon, 20 Nov 2000, Gilles Grondin wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We are looking for antibodies to mitochondrial proteins , for a } immunocytochemical study on the effect of parasites in the development of } youngs birds . Does anyone or company have mitochondrial antibodies ? } } Thank you very much . } } Gilles Grondin } } ggrond01-at-courrier.usherb.ca } } }
This is for the person asking about alternative post stains. Instead of using lead after uranyl acetate I have been very happy with bismuth (I have been looking at various biological samples embedded in Spurrs).
STOCK SOLUTION: In 10ml of DH2O add 0.2g of NaOh, 400mg of sodium tartrate, and 200mg of bismuth subnitrate. Shake until dissolved and store up to 2 weeks in the refrigerator.
WORKING SOLUTION: add 20 micro liters of stock to 1ml of DH2O and float the grids on 50 micro liter drops of this for 5 minutes and rinse with H2O.
Good Luck, Tim
Timothy G. Schneider Director of Electron Microscopy Department of Pathology Room 229 Jefferson Hall Thomas Jefferson University 1020 Locust St. Philadelphia Pa. 19107 215-503-4798 work 610-613-8170 cellular timothy.schneider-at-mail.tju.edu
Years ago I worked in a lab that had a small forced (and filtered) film dryer (with heater). It was just large enough to hold two racks of TEM negatives. I cannot find one in any of the catalogues. I can only find film dryers for 35 mm roll film. Could anyone please recommend a source of a similar dryer? (Vendors, please feel free to respond directly to me).
Thanks,
Don
______________________________________________________________________ Donald L. Lovett e-mail: lovett-at-tcnj.edu Assoc. Professor, Dept. of Biology voice: (609) 771-2876 P.O. Box 7718 fax: (609) 637-5118 The College of New Jersey Ewing, NJ 08628-0718
I am trying to find out information on a good imaging system(photographic only) that could be attached to my SEM, which is an older ABT-55. I do not have the funds to buy a newer microscope, so I would appreciate any input you all have. The cost of Polaroid film is extremely expensive, so I am trying to find a system that I could send digital images through the e-mail and/or print them instead of using this costly film. Thank you in advance.
Cathy Kelloes Microscopy Technician bp Fabrics & Fibers Unit 260 The Bluffs Austell, GA. 30168 (770) 941-1711, Ext. 3255
My friend's son is writing a report on oxytocin and called me to ask if I had or could find any images of it. Its the typical "call X, s/he is a scientist" type referal. Does anyone out there have any images, or does anyone know of a good web site I can send him to? I assume that polarization optics images of the crystalized molecule would be pretty, but anything would be more than he currently has.
Please respond to me off-list, and I will forward the info to him.
TIA, Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
The 34th Annual NESM (New England Society for Microscopy) Symposium will be held at the Newton Marriott, Newton, Massachusetts on Friday, December 1st from Noon to 9:30 pm.
The meeting will consist of 2 sessions. SESSION I (1-2:30 pm) will focus on Raman and FT-IR Spectroscopy. Speakers will include Barbara Foster-"Bridging the Microscopy-Spectroscopy Chasm", John Reffner-"Resolving Molecular Chemistry with FT-IR Microspectroscopy" and Patrick Treado-"Chemical Imaging: A Powerful Tool for Molecular Microscopy".
A Poster Session/Contest will then run concurrent with the coffee break (2:30- 3:15 pm) (details below).
SESSION II (3:15-4:45 pm) will focus on Genechip Technology. Speakers for that session will include: Eiman H. Al-Mutari-"The Human Genome Study: cDNA Micro- arrays and Signal Amplification", Joseph Paulauskis-"Microarray Technologies: Promise and Problems", and Steven Lott-"Microarray Technology: Changing the Face of Functional Genomics".
The annual business meeting/election of new officers will convene at 5:00 pm, followed by a Cocktail Hour (6-7 pm) and dinner (7-8 pm). The after dinner speaker will be Paul Hlava, MAS Tour Speaker, from Sandia National Labs whose talk will be: "Causes of Color in Minerals and Gemstones".
The DEADLINE for advance registration (and dinner reservations) is Friday, November 24th. Payment must accompany advance registration form if you wish to have dinner. The choices for dinner are: herbed crusted chicken, swordfish, and vegetarian (please choose one). Please contact Mary McCann, Treasurer, at (617) 484-7865 or by email: mccanns-at-tiac.net for further information. Send registration forms WITH payment to NESM, c/o Mary McCann, 161 Claflin Street, Belmont, MA 02478. (Registration will be held at the meeting from 12Noon-1pm, but will NOT include dinner).
Poster abstracts should be sent to: Christopher Santeufemio, Biological Director at: 57 Bancroft Street, Pepperell, MA 01463 by November 24th. Any questions, contact Chris at (978) 433-8031 or by email: csanteufemio-at-epion.com. Prizes will be awarded for Best Poster, Second and Third Place.
We hope to see you there at this most interesting meeting!
Peggy Sherwood Corresponding Secretary, NESM
-- Peggy Sherwood Lab Associate, Photopathology Wellman Laboratories of Photomedicine (W224) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) sherwood-at-helix.mgh.harvard.edu
I've been asked to do some TEM on hookworms. I've never even seen a hookworm before, let alone do EM on one. I've heard that they're tough customers when it comes to fixation.
Does anybody out there have a protocol that is fairly simple but results in good utlrastructural preservation?
There is a company called California Stainless that made film dryers that some of the EM supply houses sold. I bought one from them a number of years ago. Sorry that I don't have the contact info, but I'm sure that this is just what you want.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
-----Original Message----- From: Donald Lovett [mailto:lovett-at-tcnj.edu] Sent: Monday, November 20, 2000 9:51 AM To: Microscopy.lst Subject: Film dryers
--------------------------------------------------------------- --------- The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html
Years ago I worked in a lab that had a small forced (and filtered) film dryer (with heater). It was just large enough to hold two racks of TEM negatives. I cannot find one in any of the catalogues. I can only find film dryers for 35 mm roll film. Could anyone please recommend a source of a similar dryer? (Vendors, please feel free to respond directly to me).
Thanks,
Don
______________________________________________________________________ Donald L. Lovett e-mail: lovett-at-tcnj.edu Assoc. Professor, Dept. of Biology voice: (609) 771-2876 P.O. Box 7718 fax: (609) 637-5118 The College of New Jersey Ewing, NJ 08628-0718
On Mon, 20 Nov 2000 09:33:34 -0500 Timothy Schneider {Timothy.Schneider-at-Mail.TJU.EDU} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } This is for the person asking about alternative post stains. Instead of } using lead after uranyl acetate I have been very happy with bismuth (I have } been looking at various biological samples embedded in Spurrs). } } STOCK SOLUTION: In 10ml of DH2O add 0.2g of NaOh, 400mg of sodium tartrate, } and 200mg of bismuth subnitrate. Shake until dissolved and store up to 2 } weeks in the refrigerator. } } WORKING SOLUTION: add 20 micro liters of stock to 1ml of DH2O and float the } grids on 50 micro liter drops of this for 5 minutes and rinse with H2O. } } Good Luck, Tim } } Timothy G. Schneider } Director of Electron Microscopy } Department of Pathology } Room 229 Jefferson Hall } Thomas Jefferson University } 1020 Locust St. } Philadelphia Pa. 19107 } 215-503-4798 work } 610-613-8170 cellular } timothy.schneider-at-mail.tju.edu } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
See if an active or passive digital control & capture unit would work on your SEM. Soft Imaging makes ADDA which does active and passive very nicely. There are others which do active and some which only do passive. I prefer active. But if your system can't be adapted to active, perhaps it will support passive. If not, maybe active.
Several of the suppliers are on this list. Perhaps they will contact you. I use ADDA and like it very much. It is actually more than just SEM image capture. I use it to capture chamber TV camera video and SEM TV scan images as well as up to 4096x4096 pixel active scan SEM image files (TIFF).
gary
At 07:28 AM 11/20/00, you wrote:
} Hi, } } I am trying to find out information on a good imaging system(photographic } only) that could be attached to my SEM, which is an older ABT-55. I do not } have the funds to buy a newer microscope, so I would appreciate any input } you all have. The cost of Polaroid film is extremely expensive, so I am } trying to find a system that I could send digital images through the e-mail } and/or print them instead of using this costly film. Thank you in advance. } } Cathy Kelloes } Microscopy Technician } bp Fabrics & Fibers Unit } 260 The Bluffs } Austell, GA. 30168 } (770) 941-1711, Ext. 3255
The RMC "EMP 5160" EM tissue processor is available through Boeckeler Instruments as is the entire RMC Instrument catalog. For more information visit our website: www.rmcproducts.com
Al Coritz Sales & Service Manager RMC-Boeckeler Instruments 4650 S. Butterfield Dr. Tucson, AZ 85714 Voice: 520-745-0001 Cell: 520-465-3598 Fax: 520-745-0004 Email:Al-at-Boeckeler.com Website:RMCProducts.com
-----Original Message----- } From: Roger Moretz [mailto:rcmoretz-at-excite.com] Sent: Monday, November 20, 2000 6:00 AM To: Marek Malecki; Microscopy-at-sparc5.microscopy.com
Mareck:
I have responded to these queries before, so here goes again. The unit I have used for years is the Lynx Tissue Processor (currently available from EM Sciences). The original unit I purchased (from the manufacturer) was marvelous. A second unit (purchased when Leica was marketing it) took over 5 years to get it into reliable working condition. My guess is that EMS will provide a reliable product. The only other unit I know of is sold by RMC (recently cycled through Ventana and now Boekeler -- Tucson, AZ) that is an updated version of the old LKB design. I know of very happy users for this product, but I honestly do not know the current status under the new owners. I have attempted to get a demo of the RMC device, but RMC never followed through, and the last incarnations have occurred so quickly that I still can't provide a personal comparison.
Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. On Fri, 17 Nov 2000 00:19:10 -0800, Marek Malecki wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I would appreciate comments and suggestions on automatic sample processors } for EM. Vendors welcome. } Marek Malecki, M.D., Ph.D. } Director and Principal Investigator } } Molecular Imaging Laboratories } University of California, San Diego } } address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA92093-0368 } phone - office: 8588223373 } phone - cell: 8583443347 } fax: 8588223715 } e.mail: mmm-at-ucsd.edu } pager: 8586161420 } e.mail: mmm-at-ucsd.edu } e.pager: 1620024619-at-alphapage.airtouch.com } www site: http://mil.ucsd.edu/ } ftp site: mil1.ucsd.edu } } }
_______________________________________________________ Tired of slow Internet? Get -at-Home Broadband Internet http://www.home.com/xinbox/signup.html
Thanks again for all of the responses. They were very informative.
We primarily perform TEM on renal biopsies particularly the Bowman's capsule and glomerulus. The main ultra- structure we concentrate on is the basement membranes of the glomerulus. Less frequently we perform TEM on tumor tissues. This is done in a clinical setting.
Any other information out there in the "Netter Land" will be appreciated.
Thanks Again,
Donald G. Awbrey, HT(ASCP) QIHC Electron Microscopy / Image Analysis DonaldAwbrey-at-TexasHealth.org donaldawbrey-at-hotmail.com (817)-878-5647
At 16:37 16/11/00 -0500, you wrote: } Dear Listers, } Does anyone have an experience that they would like to share involving the } SVMicro digital camera by Sound Vision? I'm hoping to learn anything at } all, from delivery, support, service, image quality, product reliability, } etc. Thanks in advance. } Bill Roberts
We have an SVMicro attached to a Nikon Labophot-Pol petrographic microscope, with a Nikon 1x relay lens in the adaptor. We also use it with a Zeiss Jenaval. Alternatively you can just screw a lens on it and point it at something (with a tripod, preferably). The camera runs off a SCSI card in a PC.
Our experience is that the Twain interface software is a little tricky to use, and it's difficult for new users to get good images until you have done some tweaking. You usually need to turn the illumination down a bit, or use a neutral density filter. The colour balance needs to be corrected -- I use a neutral density filter as the specimen to set the neutral gray balance -- but a pale blue filter may also work. The images tend to be very contrasty unless you set the gamma level (the middle triangle on the intensity histogram) a long way to the left. Adjusting the levels tends to be very touchy, as compared to say Photoshop. However once you get the exposure time right you can get very nice RGB images, at a true pixel resolution of about 1000x800.
Thor Bostrom Analytical EM Facility Faculty of Science Queensland University of Technology (QUT) PO Box 2434, Brisbane, QLD 4001 AUSTRALIA
I may have a copy of a paper somewhere which advocated hot fixatives for nematodes because t worked better than room temp/cold fixatives. Purely on penetration? Getting through the cuticle? Come back if you're still desperate - I think it is in my cellar somewhere at home!
A colleague here is trying to determine the presence and size of microcrystallites in a glass ceramic. It is an alkali-lime borosilicate glass, which appears to be amorphous by XRD. However it has a translucent appearance which suggests that it may contain microcrystallites or some other defects, though these are not evident by optical microscopy.
The suggestions so far are: (a) light etching of a polished surface, followed by examination in a FESEM; or (b) preparation of an ion beam thinned sample for TEM.
We would welcome any other suggestions. For example, would EBSD work? And if we tried (a), is there a suitable etchant (dilute HF?) for this material?
With thanks,
Thor Bostrom Analytical EM Facility Faculty of Science Queensland University of Technology (QUT) PO Box 2434, Brisbane, QLD 4001 AUSTRALIA Email: t.bostrom-at-qut.edu.au
=-=-=-=-=-=-=-=-=-=-=-=-=-= Dr Thor Bostrom Ext. 2351 Analytical EM Facility, Faculty of Science, QUT t.bostrom-at-qut.edu.au =-=-=-=-=-=-=-=-=-=-=-=-=-=
Does anyone have suggestions how to add a label/element to Lowicryl HM20. We would like to differentiate/locate the Lowicryl in some porous structures using EDX.
thanks,
Johan Hazekamp
************************************************** Johan Hazekamp Central Analytical Science Unilever Research Vlaardingen P.O. Box 114, 3130 AC Vlaardingen The Netherlands Tel.: (31) 10-4605530 Fax: (31) 10 4605671 Email: Johan.Hazekamp-at-unilever.com
TEM examination is best and would allow identification of the crystallites. Ion milling silicates is potentially a problem. I'd suggest choosing a method that doesn't involve much, if any, ion milling, such as cleaving, crushing, or tripod polishing.
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com IBM Analytical Services; http://www.chips.ibm.com/services/asg
Thor Bostrom {t.bostrom-at-qut.edu.au} on 11/21/2000 12:42:07 AM
To: microscopy-at-sparc5.microscopy.com cc:
A colleague here is trying to determine the presence and size of microcrystallites in a glass ceramic. It is an alkali-lime borosilicate glass, which appears to be amorphous by XRD. However it has a translucent appearance which suggests that it may contain microcrystallites or some other defects, though these are not evident by optical microscopy.
The suggestions so far are: (a) light etching of a polished surface, followed by examination in a FESEM; or (b) preparation of an ion beam thinned sample for TEM.
We would welcome any other suggestions. For example, would EBSD work? And if we tried (a), is there a suitable etchant (dilute HF?) for this material?
With thanks,
Thor Bostrom Analytical EM Facility Faculty of Science Queensland University of Technology (QUT) PO Box 2434, Brisbane, QLD 4001 AUSTRALIA Email: t.bostrom-at-qut.edu.au
=-=-=-=-=-=-=-=-=-=-=-=-=-= Dr Thor Bostrom Ext. 2351 Analytical EM Facility, Faculty of Science, QUT t.bostrom-at-qut.edu.au =-=-=-=-=-=-=-=-=-=-=-=-=-=
In the early 1980s, we followed someone's suggestion to dissolve Iodoform in epoxy resin to raise its average atomic number to provide contrast with coal particles. It also provided elemental contrast.
It worked with epoxy; it setup just fine. But I am not sure how it would work Lowicryl. It does list a number of precautions listed on the MSDS.
At 11:24 AM 11/21/2000 +0100, you wrote:
} Dear All, } } Does anyone have suggestions how to add a label/element to Lowicryl HM20. } We would like to differentiate/locate the Lowicryl in some porous structures } using EDX. } } thanks, } } Johan Hazekamp } } ************************************************** } Johan Hazekamp } Central Analytical Science } Unilever Research Vlaardingen } P.O. Box 114, 3130 AC Vlaardingen } The Netherlands } Tel.: (31) 10-4605530 } Fax: (31) 10 4605671 } Email: Johan.Hazekamp-at-unilever.com }
Johan Polysciences http://www.polysciences.com/ list barium methacrylate, lead methacrylate and lead acrylate, all of which can be used to impart x-ray / electron opacity to acrylic polymers. I don't know how you would formulate Lowicryl to take advantage of this, but it looks like a possible starting point. I would be very interested to know of specific formulations for using these compounds in EM grade embedding, because I have a related application.
Best wishes Chris
Date sent: Tue, 21 Nov 2000 11:24:32 +0100 } From: "Johan Hazekamp" {Johan.Hazekamp-at-unilever.com}
Microcrystallites can be identified using back scattered electron image on a polished surface of the sample. If the microcrystallites are big enough, e.g., a few microns or larger, its chemical composition can be determined on a polished surface using electron microprobe. In some cases, the shapes and chemical composition of the phase will be enough to determine whether the phase is crystalline.
At 04:42 PM 11/21/00 +1100, Thor Bostrom wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To everyone who sent me information on SEM image systems, I want to tell you how much I appreciate it. I have received so many good suggestions and references that I now will be able to make a more knowledgeable decision. Once again, thank you so much.
Cathy Kelloes Microscopy Technician bp Fabrics & Fibers Unit 260 The Bluffs Austell, GA. 30168 (770) 941-1711, Ext. 3255
I was wondering if anyone can help me find a tiltable and rotatable specimen cartridge for a Zeiss EM 109 TEM. Vendors quite welcome!
Thanks very much for your help
Kindest Regards
Stephen Wood Meridian Scientific Services Inc. Ottawa Canada Tel: 613-836-6749 Fax: 613-836-5880 e-Fax:413-460-3007 e-mail: stephenwood-at-meridiansci.com web site: http://meridiansci.com/
Any one have a reference on the effect of pH on the osmication reaction? My attempts at a literature search on this topic have not been much success. Happy Thanksgiving.
-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
Dear Cathy, You may find that Quartz PCI, sold by Hitachi Instruments, is most suitable for passively capturing images from an older SEM. You get an image that is very close to the Poaroid image, stored on the computer in your choice of 18 different formats. Visit them at: www.quartzimaging.com. At 09:28 AM 11/20/00 -0600, you wrote: } } Hi, } } I am trying to find out information on a good imaging system(photographic } only) that could be attached to my SEM, which is an older ABT-55. I do not } have the funds to buy a newer microscope, so I would appreciate any input } you all have. The cost of Polaroid film is extremely expensive, so I am } trying to find a system that I could send digital images through the e-mail } and/or print them instead of using this costly film. Thank you in advance. } } Cathy Kelloes } Microscopy Technician } bp Fabrics & Fibers Unit } 260 The Bluffs } Austell, GA. 30168 } (770) 941-1711, Ext. 3255
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
I saw your messages in the microscopy list and was so happy to "hear" from you. I don't know if you'll remember me, I used to be a student at the Hort Department with Dr. Hazel Wetzstein and took both EM courses at the EM center in Athens.
I'm back in Brazil since 1994 and I've been back to Athens a few times after that. I visited most of the people I've known and missed seeing you there. I knew you're in Atlanta from Dr. Farmer.
How are things going with you? Do you still have horses? I see you are still working with EM, which is good!
Well, whenever you have a chance, let me know how things are going. I'm going to the US in December January and will spend some time in Athens and also will visit some friends in Atlanta. Maybe I'll have a chance to meet you again.
Take care, regards,
Adriana
Adriana P. M. Rodriguez Laboratório de Biotecnologia Vegetal CENA, Universidade de São Paulo adriana-at-cena.usp.br
Being new to this system, I don't know how this is going to work. However, I purchased an ISI-SS40 a few year ago, but I did't get any manuals with it. It appears to work ok, but there are a lot of questions. If anynoe
Knows where I can get manuals for this particular instrument I would greatly appreciate hearing from you. You may e-mail me at oconnell-at-ltu.edu or call 734-668-3309and I will get back with you.
I don't see how examination in a FESEM will be helpful. I doubt that EBSD will work because of the volume required to produce a pattern. I think that TEM is the only way to get the information. ..Russ Cook } ----------------------------------------------------------------------- } } A colleague here is trying to determine the presence and size of } microcrystallites in a glass ceramic. It is an alkali-lime borosilicate } glass, which appears to be amorphous by XRD. However it has a translucent } appearance which suggests that it may contain microcrystallites or some } other defects, though these are not evident by optical microscopy. } } The suggestions so far are: (a) light etching of a polished surface, } followed by examination in a FESEM; or (b) preparation of an ion beam } thinned sample for TEM. } } We would welcome any other suggestions. For example, would EBSD work? And } if we tried (a), is there a suitable etchant (dilute HF?) for this material? } } } With thanks, } } Thor Bostrom } Analytical EM Facility } Faculty of Science } Queensland University of Technology (QUT) } PO Box 2434, Brisbane, QLD 4001 } AUSTRALIA } Email: t.bostrom-at-qut.edu.au } } =-=-=-=-=-=-=-=-=-=-=-=-=-= } Dr Thor Bostrom } Ext. 2351 } Analytical EM Facility, } Faculty of Science, QUT } t.bostrom-at-qut.edu.au } -----------------------------------------------------------------------
---------------------------------------------------------------------- Russell E. Cook, Ph.D. Electron Microscopy Center Argonne National Laboratory Materials Science Division, Building 212 9700 South Cass Avenue Argonne, IL 60439-4838 (630)252-7194 FAX: (630)252-4289 recook-at-anl.gov
We need another specimen holder for a Philips /FEI CM 120. Gatan made a perfect holder with ±60 tilt & 360° of rotation. I am not looking for a cryoholder. Hope someone has one of these Gatan gems on their shelf that they would like to sell to us. thanks...Tom Reese, NIH
************************************************ Final Call for Papers ************************************************
This international conference will focus on the latest developments in the study of the structural and electrical properties of semiconductors by the application of transmission and scanning electron microscopy, scanning probe microscopy and X-ray techniques.
The state-of-the-art in all important subject areas will be addressed, including the characterisation of bulk and thin film as-grown materials, the study of lattice defect and impurity behaviour and the investigation of advanced semiconductor processing procedures.
Special conference sessions will concentrate on recent developments in high-resolution imaging and analytical electron microscopy, advances in SEM and SPM applications, the characteristics of epitaxial layers (including III-V nitrides), quantum wells, wires and dots, the effects of device processing treatments (including, especially, advanced silicon technology) and metal-semiconductor contacts and silicides. Prominent invited speakers will introduce each topic area.
The Proceedings of the conference will be published and the final call for papers has now been issued. Abstracts (deadline 1 DECEMBER 2000) should be submitted by E-mail to: jenny-at-rms.org.uk
Full conference information (with the invited speaker listing, etc) can be found at the conference Web site http://www.rms.org.uk/currentevents2.htm#MSMXII
The conference Chairmen are Prof Tony Cullis (a.g.cullis-at-sheffield.ac.uk) and Dr John Hutchison (john.hutchison-at-materials.ox.ac.uk) who may be contacted with any general enquiries.
************************************************ Final Call for Papers ************************************************
This international conference will focus on the latest developments in the study of the structural and electrical properties of semiconductors by the application of transmission and scanning electron microscopy, scanning probe microscopy and X-ray techniques.
The state-of-the-art in all important subject areas will be addressed, including the characterisation of bulk and thin film as-grown materials, the study of lattice defect and impurity behaviour and the investigation of advanced semiconductor processing procedures.
Special conference sessions will concentrate on recent developments in high-resolution imaging and analytical electron microscopy, advances in SEM and SPM applications, the characteristics of epitaxial layers (including III-V nitrides), quantum wells, wires and dots, the effects of device processing treatments (including, especially, advanced silicon technology) and metal-semiconductor contacts and silicides. Prominent invited speakers will introduce each topic area.
The Proceedings of the conference will be published and the final call for papers has now been issued. Abstracts (deadline 1 DECEMBER 2000) should be submitted by E-mail to: jenny-at-rms.org.uk
Full conference information (with the invited speaker listing, etc) can be found at the conference Web site http://www.rms.org.uk/currentevents2.htm#MSMXII
The conference Chairmen are Prof Tony Cullis (a.g.cullis-at-sheffield.ac.uk) and Dr John Hutchison (john.hutchison-at-materials.ox.ac.uk) who may be contacted with any general enquiries.
Hi Y'all: We're looking to buy or trade for an annealing holder for a 100CX/200CX/1200 EX TEM's (fits all of these and probably others). We hope someone has one that they haven't used in years and would like to get some money out of it Please let us know. Regards, Mike Coviello Lab Manager UT Arlington 817 272-5496
Does anyone have information about a supplier of amber colored carboy containers. We need these carboys to be about 1-2 gallon in size. They need to made of a plastic polymer that is resistant to hazardous chemicals. They are to be used to store stock film processing fluids. Any info would be greatly appreciated.
Thanks in advance.
Donald G. Awbrey, HT(ASCP) QIHC Electron Microscopy / Image Analysis DonaldAwbrey-at-TexasHealth.org donaldawbrey-at-hotmail.com (817)-878-5647
Ahoy fellow enthusiasts! Has anyone out there used a comparison microscope before? I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems to me a knob on the front that does nothing. I get one scope in once side of the field and the image from the other scope on the other side.....
I suspect the knob makes them overlap (like we have seen on the police shows but it does not seem to move the image. when they compare bullets...)
The original volume of Hayat's Principles & Techniques of Electron Microscopy (vol. 1), now long out of print, discusses this in chapter 1. He references a paper by Trump and Ericsson (Lab Invest 14:1245, 1965) as a primary reference. In fact, if you were to review Ben Trump's literature, you would find a wealth of information on the role of pH, osmolarity, fixative concentrations and types, etc. Most of this has been summarized by Hayat in various volumes and in the Glauert series. A quick on-line search at PubMed will give you enough references to bury you! However, using interlibrary loan you should be able to at least get the Glauert series, altho' I think that precious few libraries (including most EM labs) have the Hayat books. Although I can't lay my hands on a lot of books right now, I would also have thought that any basic book on TEM would cover your question, so if you have access to any of the recent books, check out the chapter on fixatives and fixation.
Roger Moretz, Ph.D. Dept. of Toxicology Boehringer Ingelheim Pharmaceuticals
On Tue, 21 Nov 2000 11:59:23 -0600, Tom Phillips wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Any one have a reference on the effect of pH on the osmication } reaction? My attempts at a literature search on this topic have not } been much success. Happy Thanksgiving. } } -- } Thomas E. Phillips, Ph.D. } Associate Professor of Biological Sciences } Director, Molecular Cytology Core Facility } } 3 Tucker Hall } Division of Biological Sciences } University of Missouri } Columbia, MO 65211-7400 } (573)-882-4712 (voice) } (573)-882-0123 (fax) }
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Hi everybody, hope someone can help me: I need to stick each other two pieces of coated metallic samples to make a XTEM observation; then I need to stick them (already together bonded!) on the lapping tool in order to thin them. I have tried to use superglue (cyanoacrylic glue) for the first operation and thermoplastic glue for the second. Unfortunately I haven't found nothing to dissolve the thermoplastic but the superglue.
Does anyone can suggest me two different glue that can be suitable and can be dissolved selectively?
Thank you in advance, Edoardo.
Dr. Eng. Edoardo Bemporad, Ph. D. Assistant Professor of Materials Science University of Rome "Roma Tre" (Italy) Dipartimento di Ingegneria Meccanica e Industriale (Department of Mechanical and Industrial Engineering) Via Vasca Navale 79 - 00146 Rome, Italy Tel: +39 06 5517.3293 Fax: +39 06 5517.3256 LIME Lab Tel: +39 06 5517.3200 LIME Web Site: http//materials.dimi.uniroma3.it/lime E-Mail:bemporad-at-uniroma3.it
I do XTEM of metal films, and have found Gatan's G-1 epoxy to be effective in creating the "sandwich". I use crystal bond low-melting wax to bond the sandwich to the polishing stub for thinning. The former is resistant to most solvents; the latter dissolves readily in acetone.
I also hear that M-bond 610 adhesive is good for gluing the halves of the sandwich together, though I have not tried this brand.
The material you should use to stick the pieces together is Epotek 353ND which is produced by Epoxy Technology. You can find information on the product on their website at www.epotek.com/optical.html. Their distributor in Italy is:
Anna Giallo Kontek Comatel S.P.A. Via Rio Vallone #5 20050 Mezzago. Milan Italy
The acetone soluble wax that you can use it our QuickStick 135. This comes in a package with 20 small sticks and is ideal for this application. I hope this helps!
David Henriks Vice President TEL: 800-728-2233 (toll free in the USA) South Bay Technology, Inc. +1-949-492-2600 1120 Via Callejon FAX: +1-949-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by "Bemporad, Edoardo" } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi everybody, hope someone can help me: I need to stick each other two pieces of coated metallic samples to make a XTEM observation; then I need to stick them (already together bonded!) on the lapping tool in order to thin them. I have tried to use superglue (cyanoacrylic glue) for the first operation and thermoplastic glue for the second. Unfortunately I haven't found nothing to dissolve the thermoplastic but the superglue.
Does anyone can suggest me two different glue that can be suitable and can be dissolved selectively?
Thank you in advance, Edoardo.
Dr. Eng. Edoardo Bemporad, Ph. D. Assistant Professor of Materials Science University of Rome "Roma Tre" (Italy) Dipartimento di Ingegneria Meccanica e Industriale (Department of Mechanical and Industrial Engineering) Via Vasca Navale 79 - 00146 Rome, Italy Tel: +39 06 5517.3293 Fax: +39 06 5517.3256 LIME Lab Tel: +39 06 5517.3200 LIME Web Site: http//materials.dimi.uniroma3.it/lime E-Mail:bemporad-at-uniroma3.it
Dear Thor, EBSP might work, if the micro-crystallites are larger than the e-beam size. The specimen preparation is the same as for rocks for EBSP: polish down to 0.01 micron or Syton (colloidal silica) level. Best to ask someone who has done it. Try the web sites of HKL (www.channel.dk) or TSL. At 04:42 PM 11/21/00 +1100, you wrote: } } A colleague here is trying to determine the presence and size of } microcrystallites in a glass ceramic. It is an alkali-lime borosilicate } glass, which appears to be amorphous by XRD. However it has a translucent } appearance which suggests that it may contain microcrystallites or some } other defects, though these are not evident by optical microscopy. } } The suggestions so far are: (a) light etching of a polished surface, } followed by examination in a FESEM; or (b) preparation of an ion beam } thinned sample for TEM. } } We would welcome any other suggestions. For example, would EBSD work? And } if we tried (a), is there a suitable etchant (dilute HF?) for this material? } } } With thanks, } } Thor Bostrom
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Hello all, I am looking at real-time X-ray radiography systems and scanning acoustic microscopes for evaluation of solder bonds in optoelectronic packages. If anyone knows of suppliers of such systems - or even better has one they like (or don't), I would really like to hear from you. This includes manufacturers too - I'm not sure I have a comprehensive list yet.
e-mail richard.beanland-at-marconi.com Tel. +44 1327 356363 Fax. +44 1327 356398 ============================================================== "The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone. Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."
It depends on who's bridge you have. The two most common are the Leitz and the AO design. An AO type would say AO, Reichart or Leica on the manufactures logo depending on how new or old it is. Cambidge decided to use the Leica name on both once they owned both designs so the Leitz could also say Leica. There are other designs but these two make up probably more than 90% of all comparison bridges. I haven't worked with any of the others in over 20 years and then only a bit.
The knob in the center of the front makes it sound more likely to be an AO type. If it is, that knob should move the center prism set so that with it turned fully clock-wise you would see all of the left scope and turned fully counter-clock-wise you would see all of the right scope. With the knob centered you would see half of each field. In some other position you would see a smaller part of one field and a larger part of the other. If it is an AO style bridge it sounds to me as if the rack and gear might be damaged so the prism is not moving. The thin dividing line you see between the two fields should move as you turn the knob to give you a smaller percentage of one field and a larger percentage of the other. This lets us compare the pattern of stria over a length of the toolmark, a bullet comparison is a type of toolmark comparison. Side by side comparison is much more useful in Firearm and toolmark comparison than overlapping fields are.
If it is a Leitz design some do not let you move the dividing line off center but have other knobs that overlap the two fields or let you view just one. These bench top Leitz bridges don't have a knob in the center of the front so this probably isn't what you have.
In modern bridges the bridges that attach to separate scopes are designed for hair and fiber comparisons and the like. Bullet ("Universal Forensic") scopes have the objectives attached into the bridges in scopes that have been built since well before I entered the field over a 1/4 century ago. They are designed for much lower power and much longer working distances. The bridges for hair and fiber comparison scopes (yes they can be used for all manor of comparison but in forensic science labs that is their primary job and the way they are usually refereed to) are quite similar to bullet scope bridge designs but are designed to be attached to separate scopes that are basically conventional compound microscopes with sub-stage lighting.
If you tell me more about the bridge you are trying to use I may be able to tell you more that can help you set it up and see if it is working properly. AO then Reichart and now Leica have always said that users should not open up bridges to try repairs, as prism alignment is critical and easily disturbed. Rebuilds are available basically only at the factory, no one else has the alignment equipment to do it right. When other places have told me they could rebuild one I found that their version of a rebuild amounted to a real good cleaning job not prism replacement or realignment. If you do have an AO type and the knob is not moving the rack the center prism is on you may need such a rebuild. I did have a knob set screw come loose on an old one quite a number of years ago. I got away with going inside and tightening it up but I would not recommend it to anyone else as these are expensive pieces of equipment and rebuilds are several thousand dollars (around 12K to 15K last time I checked several years ago).
Jim
James L. Roberts Firearm and Toolmark Examiner Ventura Co. Sheriff's Lab (805) 654-2308 James.Roberts-at-mail.co.ventura.ca.us
} } } {"COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com} 11/21/00 10:43PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Ahoy fellow enthusiasts! Has anyone out there used a comparison microscope before? I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems to me a knob on the front that does nothing. I get one scope in once side of the field and the image from the other scope on the other side.....
I suspect the knob makes them overlap (like we have seen on the police shows but it does not seem to move the image. when they compare bullets...)
Well I guess I should read the subject line as well as the body of the note and I would have seen that it was an AO bridge. Sorry for the info on the Leitz design. As indicated in my earlier message the knob should move the prism set so that you see part of one field and part of the other and what percentage of each is determined by the position of the knob. In the AO they do not "overlap."
If the knob is not moving the prism set you may have a loose set screw or you may need a bridge rebuild. Again I would not recommend going into the bridge yourself as alignment of the left, right and center prism is critical. Jim
James L. Roberts Firearm and Toolmark Examiner Ventura Co. Sheriff's Lab (805) 654-2308 James.Roberts-at-mail.co.ventura.ca.us
} } } {"COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com} 11/21/00 10:43PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Ahoy fellow enthusiasts! Has anyone out there used a comparison microscope before? I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems to me a knob on the front that does nothing. I get one scope in once side of the field and the image from the other scope on the other side.....
I suspect the knob makes them overlap (like we have seen on the police shows but it does not seem to move the image. when they compare bullets...)
I have been waiting for a response from your technical support concerning mapping . Can you have them call me.
Thank you Larry Howard
} From: "Mary Mager" {mager-at-interchange.ubc.ca} To: "Kelloes, Cathy L" {KELLOECL-at-bp.com} Cc: {Microscopy-at-sparc5.microscopy.com} Sent: Tuesday, November 21, 2000 1:07 PM
NIST used to offer a SEM calibration standard SRM-2069. I don't see this any longer. If one needs to calibrate or qualify a SEM's resolution at 250A or better, what is an appropriate method?
I've used Au on C "standards" but these are more qualitative than quantitative. The question is how to make a quantitative measurement of SEM resolution?
Earlier this month I requested information to help network an EDS system, which is a UNIX based system and operates on a Sun Sparc Station 5 with SunOS 5.3. I really appreciate all the info people have sent me and I wanted to let you know that the system has been placed on the network (TCPIP). The local IT people suggested I use an outside expert, so we did arrange for an ex-Sun employee to come in and do the job. It did take, as one suggested, 15 minutes to place the Sun on the network, but it did take another hour to (re)write some missing files (not his exact words), which allows someone on the pc network (me) to be able to ftp or take files from the Sun and bring to the pc environment. It was worth the work as we don't have to scan in the data for our reports etc. So the process does work and is pretty simple, but later I may explore some of the Windows programs suggested to facilitate these file transfers in a drag and drop style.
Thanks again,
Dave Audette david.audette-at-sylvania.com
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suggests to me that she is with the University of British Columbia, Canada and not part of Quartz/Hitachi. Are you having trouble with her or Quartz/Hitachi?
gary g.
At 10:58 AM 11/22/00, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Gary I agree with you that an objective test is hard to come by and that an industry-standard approach is lacking. I make the following observations, for what they're worth:
There is no straightforward way of making a specimen that tests the resolution of all SEMs, under all operating conditions and for all specimen types. Gold on carbon is the best choice if that is what you look at for a living. If you look at fine carbon structures on carbon it's a poor test of practical resolution, although it may indicate the general health of the instrument.
Makers differ in their approaches to resolution testing. Most employ gold on carbon, but sometimes gold on magnetic tape or dendritic crystalline gold or silver is preferred. Some measure the smallest particles visible, others measure the smallest gaps between larger particles. One practical difficulty in the former approach is that the particles need to be most abundant in a size range close to the dimensions you want to test for. SEM resolutions span at least an order of magnitude from {0.5nm for in-lens fegsem to } 5nm for tungsten sem, making production of a single universal specimen difficult. At the limits of FEGSEM resolution, ~1nm, suitably small gold particles on carbon or mag tape can be as rare as hen's teeth. Another issue is that at the limit of an instrument's resolution the image of a small particle has a bell-shaped gaussian distribution of signal, fading imperceptibly into the background. The problem here is to determine where the edges of the feature are located. Pixel size is relevant here. If the feature is only two or three pixels wide, typical of a x100k image at 1kx1k pixels, then pixel size has a dominant effect on the measured size increments - ten pixels wide or more begins to yield an approximation to continuous data, so the image size must be large or the magnification high. The standard test of resolution in microanalysis, where peaks are again approximately normal curves, is to measure peak width at half height, and one can approximate this approach in measuring a gold particle by expanding the image contrast until local background is 0 and max signal is 255, setting the threshold to 127 and measuring mean feret diameter of the feature. I think you'll find that if you apply this type of criterion you will get a more conservative estimate of resolution than is claimed by the manufacturer.
A variant of gold on carbon which we have tried is to deposit 1 nanometre gold colloid onto formvar/carbon, with 10nm gold to provide something to focus on. This is a specimen that can be characterised by TEM before examination in the SEM, so you can determine what the actual particle size distribution is (difficult to do that with gold on mag tape). FEGSEMs can "see" the 1nm gold particles because there is strong signal against low background, but as in light microscopy, where 25nm colloidal gold is readily seen under reflected illumination, may image them to a larger diameter. This larger diameter, measured using peak width at half height, thus constitutes an estimate of instrument resolving power. Another approach in principle (harder in practice) is to create perfectly square, perfectly nanometre-sharp square edges in a perfectly smooth surface of the element of your choice - silicon e.g., or a layer of another element deposited thereon - and to measure the peak width at half height of the edge transition.
Finally, theoretically it is possiible objectively to determine the limiting dimensions recorded in any image by looking at its fourier power spectrum. Biomolecular structure people regularly report such data for TEM images, so presumably the technology is availalble. Any observations on where, how, and the limitations of applying it to sem resolution measurment would be gratefully received.
Best wishes Chris
} NIST used to offer a SEM calibration standard SRM-2069. } I don't see this any longer. If one needs to calibrate or } qualify a SEM's resolution at 250A or better, what is } an appropriate method? } } I've used Au on C "standards" but these are more } qualitative than quantitative. The question is how to } make a quantitative measurement of SEM resolution? } } Vendor responses are welcome. } } Thanks, } gary g. } }
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
Gary I agree with you that an objective test is hard to come by and that an industry-standard approach is lacking. I make the following observations, for what they're worth:
There is no straightforward way of making a specimen that tests the resolution of all SEMs, under all operating conditions and for all specimen types. Gold on carbon is the best choice if that is what you look at for a living. If you look at fine carbon structures on carbon it's a poor test of practical resolution, although it may indicate the general health of the instrument.
Makers differ in their approaches to resolution testing. Most employ gold on carbon, but sometimes gold on magnetic tape or dendritic crystalline gold or silver is preferred. Some measure the smallest particles visible, others measure the smallest gaps between larger particles. One practical difficulty in the former approach is that the particles need to be most abundant in a size range close to the dimensions you want to test for. SEM resolutions span at least an order of magnitude from {0.5nm for in-lens fegsem to } 5nm for tungsten sem, making production of a single universal specimen difficult. At the limits of FEGSEM resolution, ~1nm, suitably small gold particles on carbon or mag tape can be as rare as hen's teeth. Another issue is that at the limit of an instrument's resolution the image of a small particle has a bell-shaped gaussian distribution of signal, fading imperceptibly into the background. The problem here is to determine where the edges of the feature are located. Pixel size is relevant here. If the feature is only two or three pixels wide, typical of a x100k image at 1kx1k pixels, then pixel size has a dominant effect on the measured size increments - ten pixels wide or more begins to yield an approximation to continuous data, so the image size must be large or the magnification high. The standard test of resolution in microanalysis, where peaks are again approximately normal curves, is to measure peak width at half height, and one can approximate this approach in measuring a gold particle by expanding the image contrast until local background is 0 and max signal is 255, setting the threshold to 127 and measuring mean feret diameter of the feature. I think you'll find that if you apply this type of criterion you will get a more conservative estimate of resolution than is claimed by the manufacturer.
A variant of gold on carbon which we have tried is to deposit 1 nanometre gold colloid onto formvar/carbon, with 10nm gold to provide something to focus on. This is a specimen that can be characterised by TEM before examination in the SEM, so you can determine what the actual particle size distribution is (difficult to do that with gold on mag tape). FEGSEMs can "see" the 1nm gold particles because there is strong signal against low background, but as in light microscopy, where 25nm colloidal gold is readily seen under reflected illumination, may image them to a larger diameter. This larger diameter, measured using peak width at half height, thus constitutes an estimate of instrument resolving power. Another approach in principle (harder in practice) is to create perfectly square, perfectly nanometre-sharp square edges in a perfectly smooth surface of the element of your choice - silicon e.g., or a layer of another element deposited thereon - and to measure the peak width at half height of the edge transition.
Finally, theoretically it is possiible objectively to determine the limiting dimensions recorded in any image by looking at its fourier power spectrum. Biomolecular structure people regularly report such data for TEM images, so presumably the technology is availalble. Any observations on where, how, and the limitations of applying it to sem resolution measurment would be gratefully received.
Best wishes Chris
} NIST used to offer a SEM calibration standard SRM-2069. } I don't see this any longer. If one needs to calibrate or } qualify a SEM's resolution at 250A or better, what is } an appropriate method? } } I've used Au on C "standards" but these are more } qualitative than quantitative. The question is how to } make a quantitative measurement of SEM resolution? } } Vendor responses are welcome. } } Thanks, } gary g. } }
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
The knob on the front will not affect the image, just move across the field to expose a portion or all of either the right or left image. This is used in searching the surface areas of bullets or tool marks in forensic comparisons. You're doing it right so far. Have fun.
Phil Aviles Forensic Microanalyst
} -----Original Message----- } From: "COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com } [SMTP:"COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com] } Sent: Tuesday, November 21, 2000 11:43 PM } To: MICROSCOPY-at-sparc5.microscopy.com } Subject: Has anyone out there used an AO comparison microscope } before? } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Ahoy fellow enthusiasts! } Has anyone out there used a comparison microscope before? } I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems } to } me a knob on the front that does nothing. I get one scope in once side of } the field and the image from the other scope on the other side..... } } I suspect the knob makes them overlap (like we have seen on the police } shows but it does not seem to move the image. } when they compare bullets...) } } any input? } } thanks Ed Sharpe archivist for SMECC } } }
Thank you for the clarification, but im still confused, your business card states that you are the EDX Product Development person. then would you be able to answer my questions? Why is it I cant get an answer about imaging/elemental mapping. ( See attached email)
Larry Howard
In a message dated 11/22/00 11:04:36 PM Eastern Standard Time, mmager-at-qrtz.com writes:
{ { Dear Larry,
I'm sorry if our technical support team did not respond to your request for information on our mapping option. The passive, full position-tagged mapping option is just now finished and the team is working very hard to get the information out to all the customers that requested it. I will be happy to phone you and answer any questions that you may have, if you will send me your phone number by return e-mail.
In answer to the subject of your e-mail, I work as a microscopist for the University of British Columbia and conduct any correspondance with the MSA Listserver in that capacity. I am also an EDX technical consultant for the X-ray Division of Quartz Imaging Corp. I have little to do with the Quartz PCI product except as a user and friend of the owner of Quartz.At 01:58 PM 11/22/00 -0500, you wrote: } Mary, } } I have been waiting for a response from your technical support concerning } mapping . Can you have them call me. } } Thank you } Larry Howard
Best regards, Mary
Mary Mager EDX Product Specialist Quartz Imaging Corp. 810 - 1112 West Pender Street Vancouver, B.C. Canada V6E 3X5 tel: 604-488-3911 fax: 604-488-3922 e-mail: mmager-at-qrtz.com --------------------
Dear Larry,
I'm sorry if our technical support team did not respond to your request for information on our mapping option. The passive, full position-tagged mapping option is just now finished and the team is working very hard to get the information out to all the customers that requested it. I will be happy to phone you and answer any questions that you may have, if youwill send me your phone number by return e-mail.
In answer to the subject of your e-mail, I work as a microscopist for the University of British Columbia and conduct any correspondance with theMSA Listserver in that capacity. I am also an EDX technical consultant for the X-ray Division of Quartz Imaging Corp. I have little to do with the Quartz PCI product except as a userand friend of the owner of Quartz.At 01:58 PM 11/22/00 -0500, youwrote: } Mary, } } I have been waiting for a response from your technical supportconcerning } mapping . Can you have them call me. } } Thank you } Larry Howard
Best regards, Mary
Mary Mager EDX Product Specialist Quartz Imaging Corp. 810 - 1112 West Pender Street Vancouver, B.C. Canada V6E 3X5 tel: 604-488-3911 fax: 604-488-3922 e-mail: mmager-at-qrtz.com
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the way people with TEMs measure the resolution is pretty straight forward: They take an image and do a Fourier transform (either through software FFT or with an optical diffractometer). The FFT then shows essentially the frequencies that are on the image. For a TEM you can also calculate the Contrast Transfer Function (CTF), which essentially gives you the same information. By comparing the two, you can thus find out, what the resolution of the microscope is. Since the CTF is a fairly complex function, there are different "resolutions": Point resolution (corresponds to the frequency where the CTF becomes zero the first time), or the "lattice resolution" (where the intensity of the oscillations goes below a certain threshold).
For an SEM, I assume there is only a gradually declining FFT (Intensity vs. spatial frequency). Unless someone "defines" where the cut-off is (for example where the intensity has gone down to 20%), I don't think you can really determine the resolution with certainty. The sample also needs to be defined (for example a sharp metal edge, as any charging may affect the resolution. Also the material itself may have to be defined, as edge effects may influence the measurement. Finally, the sample must show structure below the resolution limit of the microscope, otherwise you measure the "resolution" of your sample. In TEMs that is fairly simple: use an amorphous material. For SEM samples I don't know what could be used. Something with atomic structure on the surface? A surface reconstructed semiconductor?
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk] Sent: Thursday, November 23, 2000 2:24 AM To: Gary Gaugler Cc: microscopy-at-sparc5.microscopy.com
Gary I agree with you that an objective test is hard to come by and that an industry-standard approach is lacking. I make the following observations, for what they're worth:
There is no straightforward way of making a specimen that tests the resolution of all SEMs, under all operating conditions and for all specimen types. Gold on carbon is the best choice if that is what you look at for a living. If you look at fine carbon structures on carbon it's a poor test of practical resolution, although it may indicate the general health of the instrument.
Makers differ in their approaches to resolution testing. Most employ gold on carbon, but sometimes gold on magnetic tape or dendritic crystalline gold or silver is preferred. Some measure the smallest particles visible, others measure the smallest gaps between larger particles. One practical difficulty in the former approach is that the particles need to be most abundant in a size range close to the dimensions you want to test for. SEM resolutions span at least an order of magnitude from {0.5nm for in-lens fegsem to } 5nm for tungsten sem, making production of a single universal specimen difficult. At the limits of FEGSEM resolution, ~1nm, suitably small gold particles on carbon or mag tape can be as rare as hen's teeth. Another issue is that at the limit of an instrument's resolution the image of a small particle has a bell-shaped gaussian distribution of signal, fading imperceptibly into the background. The problem here is to determine where the edges of the feature are located. Pixel size is relevant here. If the feature is only two or three pixels wide, typical of a x100k image at 1kx1k pixels, then pixel size has a dominant effect on the measured size increments - ten pixels wide or more begins to yield an approximation to continuous data, so the image size must be large or the magnification high. The standard test of resolution in microanalysis, where peaks are again approximately normal curves, is to measure peak width at half height, and one can approximate this approach in measuring a gold particle by expanding the image contrast until local background is 0 and max signal is 255, setting the threshold to 127 and measuring mean feret diameter of the feature. I think you'll find that if you apply this type of criterion you will get a more conservative estimate of resolution than is claimed by the manufacturer.
A variant of gold on carbon which we have tried is to deposit 1 nanometre gold colloid onto formvar/carbon, with 10nm gold to provide something to focus on. This is a specimen that can be characterised by TEM before examination in the SEM, so you can determine what the actual particle size distribution is (difficult to do that with gold on mag tape). FEGSEMs can "see" the 1nm gold particles because there is strong signal against low background, but as in light microscopy, where 25nm colloidal gold is readily seen under reflected illumination, may image them to a larger diameter. This larger diameter, measured using peak width at half height, thus constitutes an estimate of instrument resolving power. Another approach in principle (harder in practice) is to create perfectly square, perfectly nanometre-sharp square edges in a perfectly smooth surface of the element of your choice - silicon e.g., or a layer of another element deposited thereon - and to measure the peak width at half height of the edge transition.
Finally, theoretically it is possiible objectively to determine the limiting dimensions recorded in any image by looking at its fourier power spectrum. Biomolecular structure people regularly report such data for TEM images, so presumably the technology is availalble. Any observations on where, how, and the limitations of applying it to sem resolution measurment would be gratefully received.
Best wishes Chris
} NIST used to offer a SEM calibration standard SRM-2069. } I don't see this any longer. If one needs to calibrate or } qualify a SEM's resolution at 250A or better, what is } an appropriate method? } } I've used Au on C "standards" but these are more } qualitative than quantitative. The question is how to } make a quantitative measurement of SEM resolution? } } Vendor responses are welcome. } } Thanks, } gary g. } }
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
I would like to ask you how it is possible to identify the inflammatory cells in paraffin embedded human liver specimens by means of immunohistochemistry technique. Is there a better antibody?
I don't see why this is being conducted on the list.
Can't you guys sort it out in private?
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Thanks to James and all others that replied and helped shed some light on this matter
What it appears we have is bridge for an AO hair and fiber comparison scope. It seems to be fixed in the split mode, which is fine, as it will show a half for each scope. We have 2 model 10 AO scopes in the Museum's parts stash and I put enough stuff together to make 2 with the exception that I am missing the parts for both of the mechanical stages... Any one have some AO carcasses out there we can raid for parts?
thanks Ed Sharpe archivist for SMECC
{ { Subj: Re: Has anyone out there used an AO comparison microscope before? Date: 11/22/00 1:33:22 PM US Mountain Standard Time From: James.Roberts-at-mail.co.ventura.ca.us (James Roberts) To: COURYHOUSE-at-sparc5.microscopy.com, MICROSCOPY-at-sparc5.microscopy.com
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It depends on who's bridge you have. The two most common are the Leitz and the AO design. An AO type would say AO, Reichart or Leica on the manufactures logo depending on how new or old it is. Cambidge decided to use the Leica name on both once they owned both designs so the Leitz could also say Leica. There are other designs but these two make up probably more than 90% of all comparison bridges. I haven't worked with any of the others in over 20 years and then only a bit.
The knob in the center of the front makes it sound more likely to be an AO type. If it is, that knob should move the center prism set so that with it turned fully clock-wise you would see all of the left scope and turned fully counter-clock-wise you would see all of the right scope. With the knob centered you would see half of each field. In some other position you would see a smaller part of one field and a larger part of the other. If it is an AO style bridge it sounds to me as if the rack and gear might be damaged so the prism is not moving. The thin dividing line you see between the two fields should move as you turn the knob to give you a smaller percentage of one field and a larger percentage of the other. This lets us compare the pattern of stria over a length of the toolmark, a bullet comparison is a type of toolmark comparison. Side by side comparison is much more useful in Firearm and toolmark comparison than overlapping fields are.
If it is a Leitz design some do not let you move the dividing line off center but have other knobs that overlap the two fields or let you view just one. These bench top Leitz bridges don't have a knob in the center of the front so this probably isn't what you have.
In modern bridges the bridges that attach to separate scopes are designed for hair and fiber comparisons and the like. Bullet ("Universal Forensic") scopes have the objectives attached into the bridges in scopes that have been built since well before I entered the field over a 1/4 century ago. They are designed for much lower power and much longer working distances. The bridges for hair and fiber comparison scopes (yes they can be used for all manor of comparison but in forensic science labs that is their primary job and the way they are usually refereed to) are quite similar to bullet scope bridge designs but are designed to be attached to separate scopes that are basically conventional compound microscopes with sub-stage lighting.
If you tell me more about the bridge you are trying to use I may be able to tell you more that can help you set it up and see if it is working properly. AO then Reichart and now Leica have always said that users should not open up bridges to try repairs, as prism alignment is critical and easily disturbed. Rebuilds are available basically only at the factory, no one else has the alignment equipment to do it right. When other places have told me they could rebuild one I found that their version of a rebuild amounted to a real good cleaning job not prism replacement or realignment. If you do have an AO type and the knob is not moving the rack the center prism is on you may need such a rebuild. I did have a knob set screw come loose on an old one quite a number of years ago. I got away