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From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 1 Dec 2000 08:41:20 +0100
Subject: To D.Sicard : Raith adress

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To D. Sicard

Here are two adresses of Raith GmbH, in the USA and in Germany :
Raith INC.
6 Beech Road
Islip
NY 11751-4907
Phone (516)224-1764
Fax (516)224-2620
E-mail 73164.1330-at-compuserve.com

Headquarter in Germany

Raith GmbH
Hauert 18
D-44227 Dortmund
Phone +49 (0)231 975000-0
Fax (0)231 975000-5
E-mail Raith-at-Raith.de

Web site http//WWW.raith.de

I hope the american adress is still right. The german one is good, and you
should be able to verify on the web site.

Bye

J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Fri Dec 1 03:27:10 2000



From: loy96-at-eeng.dcu.ie (Tom)
Date: Fri, 1 Dec 2000 03:23:17 -0600 (CST)
Subject: Know Your Rights

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=================================
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From daemon Fri Dec 1 04:30:07 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 1 Dec 2000 11:25:57 +0100
Subject: Hitachi S4700 and Jeol 6700F

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Dear collègues

We have tested these two FE-SEM and we want to have other opinions about
the differences between these two apparatus. Did you see a big difference
in resolution at low energy (1-2 keV). What about the practical use of the
"In Lens" detector of the S4700 ? Does it really bring more information
than the others ? I think there are some 6700F in Far East (and in the
States ?), but no one in Europe. We are interested in all you can say
about these two SEMs.

Sincerely Yours

J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX
FRANCE

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Fri Dec 1 05:28:51 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 1 Dec 2000 11:24:34 +0000
Subject: Re: Hitachi S4700 and Jeol 6700F

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Jacques
We have also evaluated these two microscopes, and I would be interested to
compare notes with you, but not online.
Chris

}
} Dear collègues
}
} We have tested these two FE-SEM and we want to have other opinions about
} the differences between these two apparatus. Did you see a big difference
} in resolution at low energy (1-2 keV). What about the practical use of the
} "In Lens" detector of the S4700 ? Does it really bring more information
} than the others ? I think there are some 6700F in Far East (and in the
} States ?), but no one in Europe. We are interested in all you can say
} about these two SEMs.
}
} Sincerely Yours
}
} J. Faerber
} IPCMS-GSI
} 23, rue de Loess
} 67037 Strasbourg CEDEX
} FRANCE
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)0 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
}
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Fri Dec 1 05:43:33 2000



From: AL :      one_2_one_sims-at-yahoo.com
Date: Fri, 1 Dec 2000 05:40:40 -0600 (CST)
Subject: Hi there

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From daemon Fri Dec 1 07:28:19 2000



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Fri, 1 Dec 2000 08:12:17 -0500
Subject: TEM - Availability of TEM Negative Sleeves/sheets

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December 1, 2000

Thanks to everyone who responded to my cry for help.

To summarize my findings and your suggestions/comments;
Kodak has discontinued the sale of transparent sleeves, CAT 150 3812,
however, that item with the same catalogue number is now being sold by
Tiffen (1-800-368-6257). Of course these sleeves may also be available from
other outlets, eg. National Graphic Supply (1-800-223-7130). A number of
microscopists are using and have suggested I try the polyview sheets which
hold 6 TEM negatives and fit nicely into a 3-ring binder. These sheets, cat.
#1310/3406 are sold by Negafile (1-888-881-6435) but again may also be
available from other outlets, eg. Ted Pella (916-243-2200), in Canada
(1-800-243-7765).

Regards,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com



From daemon Fri Dec 1 09:21:56 2000



From: simon watkins :      swatkins+-at-pitt.edu
Date: Fri, 01 Dec 2000 10:06:14 -0500
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
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Senior technician/manager for Electron Microscopy Laboratory
Managerial position for state-of-the-art electron microscopy facility within
the Center for Biologic Imaging, University of Pittsburgh Medical School.
Duties include overseeing and conducting the processing and preparation of
samples for Transmission and Scanning Electron Microscopy and specialized
techniques including cryosectioning and immunoelectron microscopy.
Incumbent will help with training and assisting students and faculty on
equipment, oversee general lab procedures including budget, supply, purchase
orders, photography and quality control. Prior experience in electron
microscopy essential. Bachelor’s or Master’s degree or equivalent desired.
The successful applicant will be responsible for directing the 3 other staff
in the EM component of the center
The Center is a nationally recognized imaging resource using and developing
a complete array of imaging technologies using both light and electron
optics. More details about the center can be found at our web site
(http://sbic6.sbic.pitt.edu):. This position is within the EM component of
the CEnter. We have a full array of prep equipment from freeze fracture to
ultracryomicrotomes. Our microscope base includes two transmission and two
SEM scopes including a new field emission gun SEM.
If you would like further information please contact Dr. Donna Beer Stolz or
myself. Please send applications electronically to dstolz+-at-pitt.edu

---------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor, Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330

-----------------------------------



From daemon Fri Dec 1 10:57:15 2000



From: HOWARD L. MULHERN :      mulhern-at-hub.tch.harvard.edu
Date: Fri, 01 Dec 2000 11:47:14 -0500
Subject: MSA CERTIFICATION

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Does anyone know what happened to MSA Certification? I stopped at their
booth in August at MSA 2000 and was assured that things getting "back
on track" but so far I've heard nothing from them.

Howard Mulhern
mulhern-at-a1.tch.harvard.edu




From daemon Fri Dec 1 10:59:41 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 01 Dec 2000 11:55:22 -0500
Subject: Raith(USA) Phone Number Correction

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

J. Faerber wrote:
==================================
To D. Sicard

Here are two adresses of Raith GmbH, in the USA and in Germany :
Raith INC.
6 Beech Road
Islip
NY 11751-4907
Phone (516)224-1764 { { {NOT CORRECT
Phone (631) 224-1764 { { {CORRECT
Fax (516)224-2620 { { {NOT CORRECT
FAX (631)224-2620 { { { {CORRECT
E-mail 73164.1330-at-compuserve.com
======================================
There has been an area code change and the old numbers absolutely won't work
!

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================







From daemon Fri Dec 1 11:02:39 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 01 Dec 2000 08:57:57 -0800
Subject: MIL-STD-883 Method 2018 - SEM Inspections

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Does anyone already have a prepared procedure according
to paragraphs 2.2 and 2.3 of MIL-STD-883E, Method 2018.3
they would share with me? What I am wondering is whether
this procedure is detailed (this knob, that button, etc.) or
more general in nature?

Based on the responses I did not receive from my earlier
post about quantitative measurement of SEM resolution,
it looks like paragraph 2 cannot be met. It states a
resolution of 250A or less. Semantically, I'm sure it
meant 250A or a lesser number. A resolution of 250A
or less would tend to mean a poorer resolution than
250A and hence a larger number. Probably picky
on my part.

Would appreciate any inputs regarding this MIL-STD.

Thanks,
gary g.



From daemon Fri Dec 1 12:18:28 2000



From: Tom Januszewski :      tom.januszewski-at-email.swmed.edu
Date: Fri, 01 Dec 2000 11:43:33 -0600
Subject: Lowicryl K4M processing

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Hi Listservers,

I've recently started lowicryl
(K4M) processing of mouse tissue. I
am having good success with
skeletal muscle, heart, kidney and
lung but stomach, spleen, pancreas
and adrenal had large holes and cut
poorly. In addition, a yeast pellet
that was resuspended in 2% agarose
prior to processing was not well
infiltrated.

Processing procedure:
3% paraformaldehyde/0.5% glut.
fixation for 60 minutes with
rotation (RT)
Wash 3X for tem minutes each with
PBS buffer
Dehydrate 30% ETOH for 30 min. at
0C
50% ETOH for 60
min. at -20C
80% ETOH for 60
min. at -35C
100% ETOH (freshly
opened bottle) for 60 min. at -35C
100% ETOH for 60
min. at -35C
ETOH/K4M 1:1 for 60
min. at -35C
ETOH/K4M 1:2 for 60
min. at -35C
K4M for 60 min.
at -35CC
K4M overnight at
-35C
K4M 6-8 hrs at -35C

Embed with fresh
resin
Specimens are constantly rotated in
freezer. All alcohols and resins
are prechilled prior to use. Resin
is mixeed by gently bubbling Argon
gas to dissolve initiator.
Polymerize at -35C overnight using
UV light
Raise temp to 0C and polymerize
additional two days with UV
Polymerize at room temp. for two
days using UV

Any thoughts on what I'm doing
wrong or things that I can change
would be greatly appreciated.

Tom Januszewski
Senior Electron Microscopist
UT Southwestern Medical Center at
Dallas
Dallas, TX 75390-9039
214-648-7291
FAX: 214-648-6408
Email:
tom.januszewski-at-utsouthwestern.edu



From daemon Fri Dec 1 13:04:07 2000



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Fri, 1 Dec 2000 13:48:26 -0500
Subject: Looking for address of Company

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December 1, 2000

I have an old business card (how old I don't know) for Raith. The contact
name is George Lanzarotta at (516) 293-0870. The address is (was?) 70C
Carolyn Boulevard, Farmingdale, NY 11735.

Good luck,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com


-----Original Message-----
} From: Ladd Research [mailto:sales-at-laddresearch.com]
Sent: Thursday, November 30, 2000 4:26 PM
To: microscopy-at-sparc5.microscopy.com


Could anyone provide me with a phone number, e-mail or fax number for
the company "RAITH USA" It is a Germany Company and I thought there was
a distrbutor in the US

Thanks

D. Sicard
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955


From daemon Fri Dec 1 19:11:28 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Fri, 01 Dec 2000 17:03:47 -0800
Subject: Pt evaporation

Contents Retrieved from Microscopy Listserver Archives
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Just got a new vacuum evaporator after many years of using our Balzers 400
for the same purpose. Course now we don't have e guns and must use a
little tungsten basket and a bit of Pt wire. I did this many years ago but
now I am having a problem I don't recall encountering back then. The
tungsten basket breaks just as the wire melts. I suspect the energy on
phase transition is cooling the tungsten and causing it to break but I
could be way off there. Anyone else see this problem and know the solution?

TIA


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Fri Dec 1 20:18:14 2000



From: mtl :      mtl-at-njcc.com
Date: Fri, 01 Dec 2000 21:16:04 -0500
Subject: Re: Thesis question

Contents Retrieved from Microscopy Listserver Archives
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Giselle
You probably should check out the Journal of Fuel or "Fuel". "Carbon"
occasionally has articles linking the mineral content of the feedstock with the
resultant char. The Northern Carbon Research Laboratories, U. of
Newcastle-upon-tyne used to publish regularly in this field. Penn. State also
has a very active Fuel Science Dept.
Good Luck.

J. Roy Nelson, Ph.D.
Material Testing Laboratory
mtl-at-njcc.com

giselle melville wrote:

}
} Good afternoon
}
} My name is Giselle. I am a graduate student working on my thesis. My
} thesis topic is "Methods of preparation of petrographic thin sections for
} Electron Microscopy". I have a collection of thin sections (chert) and I am
} preparing the sections for analysis by a TEM 1200. The thin sections are
} initially prepared by methods Ultramicrotomy and Ion Milling. TEM
} parameters used will be electron diffraction and elemental composition to
} obtain identification of the mineral and microstructures. I need more
} information (references) on recent literature (1998-2000) on my subject or
} similiar work done using these methods for analyis by TEM.
}
} I need your help!!!
}
} Thank you for your time.



From daemon Sat Dec 2 02:00:19 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Sat, 2 Dec 2000 01:53:26 -0600
Subject: Ask-A-Microscopist DIC vs phase for viewing living organisms

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I am helping a friend find a scope. I came across a Zetopan that may fit
his budget. I use a Nachet DIC for viewing living organisms and find it
very satisfactory. Could anyone help him with the versatility of a DIC
Zetopan. I know my Nachett works as a bright field, DIC and sort of
polarizing scope lacking a rotating stage and 1/4 and 1/2 wave plates. It
is the scope I use all the time. Unless I am doing darkfield.

Having never been around a Zetopan I can't really answer his questions.

Thanks
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger


} } I my quest to find a better 'scope I have come across (with the help
} } of one of the list members) a Reichert Zetopan DIC scope that *might*
} } be available at in my price range. Previously I was just looking for
} } a nice phase contrast job. I have worked with phase before but not
} } DIC and would like to solicit other opinions as to whether this is
} } the right scope for me. This is going to pretty much blow my budget
} } so I want to be sure.
} }
} } I will be using this primarily for observing living organisms
} } (protozoa, algae, etc). I have been looking at a lot of photos of
} } protozoa taken using Nomarski DIC. While the photos are striking, I
} } keep asking myself "but what does it really look like". The problem
} } is the false 3D effect. I'm never sure what the part of the shape is
} } real and what is generated. Doesn't this ever bother you?
} }
} } I have been told that it is possible to adjust out the 3-D effect but
} } I'm not sure what kind of image you are left with. I would assume
} } something in-between bright field and DIC. Anyone have any experience
} } with this?
} }
} } I think if the Zetopan is unable to easily switch between a DIC image
} } and a bright (or dark) field image then I may pass on it. I need the
} } more traditional illumination as well to see what the real shape is.
} } It really makes the Zetopan too special purpose for someone like
} } myself. I think I would probably be happier with a less
} } sophisticated scope that can do phase, bright, and dark field (and
} } maybe oblique lighting as well).
} }
} } I would appreciate any thoughts you all may have.
} }
} } Bill
} } --
} } Bill Tschumy
} } Otherwise -- Austin, TX
} } bill-at-otherwise.com




From daemon Sat Dec 2 10:30:43 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 02 Dec 2000 11:24:29 -0500
Subject: Pt evaporation

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Rick A. Harris wrote:
================================================================
Just got a new vacuum evaporator after many years of using our Balzers 400
for the same purpose. Course now we don't have e guns and must use a
little tungsten basket and a bit of Pt wire. I did this many years ago but
now I am having a problem I don't recall encountering back then. The
tungsten basket breaks just as the wire melts. I suspect the energy on
phase transition is cooling the tungsten and causing it to break but I
could be way off there. Anyone else see this problem and know the solution?
================================================================
There really is no perfect "solution". The precious group metals tend to
alloy quite readily with tungsten, Au and Pd very quickly, and I believe Pt
is quite similar in behavior.

You can reduce the magnitude of the "problem" by making sure that when you
install the tungsten basket, the wire itself is under zero stress. This
might mean a bit more time adjusting the holders and posts than you might be
doing now. Also, ball up the Pt wire and press into the very bottom of the
basket, this will enable you to get the most evaporated in the shortest
possible time period, before the wire basket breaks. And you want to use
more of a "flash" evaporation than a long slow continuous kind of
evaporation.

Another approach, but one we would not recommend, is to use a thicker wire
basket but many EM samples could be heat damaged from the additional radiant
heat. 20 mil wire is our recommended basket wire diameter.

Hope this information will be helpful.

Disclaimer: SPI Supplies manufactures tungsten wire baskets for vacuum
evaporation.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================



From daemon Sat Dec 2 21:40:12 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Sat, 2 Dec 2000 21:28:05 -0600
Subject: a data base program

Contents Retrieved from Microscopy Listserver Archives
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Does anyone out there in microscopy land know of a computer database program
( preferably PC) that has the scientist in mind and not the business man. I
have Excel, Access, and Project 2000 but they are orientated to calculate
dollars and cent not milliliters and millimeters if you know what I mean.

Ron Austin
Dept of Pathology
LSU Medical Center
Shreveport, LA
rla-at- mindspring.com
318-675-4557



From daemon Sun Dec 3 06:21:40 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Sun, 3 Dec 2000 14:53:37 -0600
Subject: Re: Ask-A-Microscopist DIC vs phase for viewing living organisms

Contents Retrieved from Microscopy Listserver Archives
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yes I am using databases from dbase II on cp/m systems

ricardo

----- Original Message -----
} From: "Vr. Richard Bejsak-Collorado-Mansfeld" {ricardo-at-ans.com.au}
To: "Ronald Austin" {rla-at-mindspring.com}
Sent: Sunday, December 03, 2000 11:16 PM


Greetings,
Nomarski optics are widely considered appropriate for viewing
living cells. While it is true that the "3-D" look might or might not
be present in the cell (more about that later), one must remember
that in phase contrast there is the famous "halo", representing an
out of focus reverse-contrast image that certainly is not present in
the cell.

Nomarski optics provide contrast by means of a gradient in
optical path (= sample thickness times refractive index). That is
anywhere in the sample where the local gradient in optical path is
different than the background (which typically would have a zero
gradient) the intenstity of the image is different than the
background. When the gradient is positive the intensity is brighter
than background, when the gradient is negative then the intensity is
darker than background. The most obvious gradients of optical path
for a living cell suspended in an aqueous medium is the change in
thickness that happens at the cell's edges: at one edge the cell is
getting thicker (= postive gradient, hence bright) and at the
opposite edge the cell gets thinner (= negative gradient, hence
dark). Consquently, the topographic appearance does relate to the
sample in a real, predictable way.

Nomarski optics can be harder to adjust properly than phase
contrast. In phase, you have the phase ring in the condenser and this
has to be centered with the phase ring in the objective. That's all
you have to do. For Nomarski, you have to have first crossed
polarizers, you have to know where they are and be able to rotate at
least one of them. THen, the system also has two Wallason prisms, one
in the condenser and one above the objective. The Wallaston prism has
an optical axis and this axis of each Wallaston must be parallal and
at 45 degrees to the analyzer/polarizer alignment. Different
implementations of Nomarski differ vastly in the accessibility of the
elements and the ease of their aligment. In the good designs, it is
no trouble at all. But of course, one should know where each one is,
what it does and how it should be aligned.

Changing between nomarki and brightfied is no trouble, you
just remove the analyzer or polarizer from the optical path.

One important fact about Nomarski arises from the optical
axis of the Wallastons. This defines the direction used to assess the
gradient in optical path. The optics cannot detect gradients that run
perpendicular to the Wallaston's optical axis. This means that
features revealed in the image depend on the orientation of the cell.
A rotatable stage is therefore very desirable. What is more,
depending on your purpose, and the types of cells your are looking
at, the directional properties of Nomarski will be more or less of a
problem.

I have no experience with a Zetopan. If at all possible have
demonstration before you buy. Finally, in many scopes you can
exchange conderser elements so that you can do either phase or
Nomarski. Thus, you could expand your repertoire later, as needs
arise. I would be useful to find out if the Zetopan lets you do that.

Hope this helps,
Tobias Baskin

}
} I am helping a friend find a scope. I came across a Zetopan that may fit
} his budget. I use a Nachet DIC for viewing living organisms and find it
} very satisfactory. Could anyone help him with the versatility of a DIC
} Zetopan. I know my Nachett works as a bright field, DIC and sort of
} polarizing scope lacking a rotating stage and 1/4 and 1/2 wave plates. It
} is the scope I use all the time. Unless I am doing darkfield.
}
} Having never been around a Zetopan I can't really answer his questions.
}
} Thanks
} Gordon
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger
}
}
} } } I my quest to find a better 'scope I have come across (with the help
} } } of one of the list members) a Reichert Zetopan DIC scope that *might*
} } } be available at in my price range. Previously I was just looking for
} } } a nice phase contrast job. I have worked with phase before but not
} } } DIC and would like to solicit other opinions as to whether this is
} } } the right scope for me. This is going to pretty much blow my budget
} } } so I want to be sure.
} } }
} } } I will be using this primarily for observing living organisms
} } } (protozoa, algae, etc). I have been looking at a lot of photos of
} } } protozoa taken using Nomarski DIC. While the photos are striking, I
} } } keep asking myself "but what does it really look like". The problem
} } } is the false 3D effect. I'm never sure what the part of the shape is
} } } real and what is generated. Doesn't this ever bother you?
} } }
} } } I have been told that it is possible to adjust out the 3-D effect but
} } } I'm not sure what kind of image you are left with. I would assume
} } } something in-between bright field and DIC. Anyone have any experience
} } } with this?
} } }
} } } I think if the Zetopan is unable to easily switch between a DIC image
} } } and a bright (or dark) field image then I may pass on it. I need the
} } } more traditional illumination as well to see what the real shape is.
} } } It really makes the Zetopan too special purpose for someone like
} } } myself. I think I would probably be happier with a less
} } } sophisticated scope that can do phase, bright, and dark field (and
} } } maybe oblique lighting as well).
} } }
} } } I would appreciate any thoughts you all may have.
} } }
} } } Bill
} } } --
} } } Bill Tschumy
} } } Otherwise -- Austin, TX
} } } bill-at-otherwise.com

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Sun Dec 3 15:30:59 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 3 Dec 2000 13:17:14 -0800
Subject: Microscopy education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm pleased to announce that a revised, up-to-date version of "Children's
Microscopy: a Bibliography" will be mailed as a supplement to the December
issue of Microscopy Today; watch for it. It has brief reviews and ordering
information on books, videos, CD-ROMs and websites. If you can't use it
yourself, PLEASE give it to a teacher, or a parent, or a Scout leader, or a
librarian - anyone who can use it to introduce children to the microworld.

If you aren't a Microscopy Today subscriber {microtoday-at-mindspring.com} you
can read the same text soon on Project MICRO's website (URL below). But
it's a tightly packed 16 pages, so it will be easier to browse the printed
version.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun Dec 3 16:36:55 2000



From: Raymond Bennett :      rbennett-at-hortresearch.co.nz
Date: Mon, 4 Dec 2000 11:31:32 +1300
Subject: Change of Address

Contents Retrieved from Microscopy Listserver Archives
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Howdy;

Our email address has changed to
rbennett-at-hortresearch.co.nz


Thanks

Raymond Bennett
Keith Williamson Electron Microscope Unit
Hort+Research
Private Bag 11030
Palmerston North
NEW ZEALAND


From daemon Sun Dec 3 23:08:55 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Sun, 3 Dec 2000 23:00:39 -0600
Subject: a data base program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I wish to thank all of you who responded to my question about a database
program. I shall look into the various suggestion in the near future.

Ron Austin
Dept of Pathology
LSU Medical Center
Shreveport, LA



From daemon Mon Dec 4 08:38:08 2000



From: Robert Price :      spike-at-auburn.edu
Date: Mon, 4 Dec 2000 08:30:31 -0600
Subject: Need help with EDS detector

Contents Retrieved from Microscopy Listserver Archives
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We are looking for a second hand EDS detector. Please email me off list.
Thanks,
Robert
C.A.V.E.
Auburn University



From daemon Mon Dec 4 09:26:35 2000



From: Rachel Spicer :      spicer-at-oeb.harvard.edu
Date: Mon, 04 Dec 2000 10:21:49 -0500
Subject: lignin autofluorescence

Contents Retrieved from Microscopy Listserver Archives
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Hello -

Does anyone out there have suggestions on histochemical stains (or other
techniques) to reduce the autofluorescence of lignin? I'm using DAPI
staining on wood (xylem) sections, which have highly lignified cell walls,
and I would like to reduce background fluorescence. Thanks.

Rachel

******************************************
Rachel Spicer
Biological Laboratories 3119
Organismic and Evolutionary Biology
Harvard University
16 Divinity Avenue
Cambridge, MA 02138

(617) 496-3580 (phone)
(617) 496-5854 (fax)
spicer-at-oeb.harvard.edu
******************************************



From daemon Mon Dec 4 09:28:14 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 4 Dec 2000 10:26:06 -0500
Subject: RE: a data base program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is relatively easy to program in Microsoft's Visual Basic 6 using
databases. It is one of that program's strengths. You can tailor any
properties that you want. It is easy to set it up to be compatible with
Microsoft's Access, but you can have it set up for any ODBC database. You
can actually set up a working database program by just using the Form Wizard
that comes with VB6.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Ronald Austin [mailto:rla-at-mindspring.com]
Sent: Saturday, December 02, 2000 10:28 PM
To: Microscopy Society of America
Subject: a data base program


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
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--------.


Does anyone out there in microscopy land know of a computer
database program
( preferably PC) that has the scientist in mind and not the
business man. I
have Excel, Access, and Project 2000 but they are orientated
to calculate
dollars and cent not milliliters and millimeters if you know
what I mean.

Ron Austin
Dept of Pathology
LSU Medical Center
Shreveport, LA
rla-at- mindspring.com
318-675-4557




From daemon Mon Dec 4 09:45:56 2000



From: Philip Koeck :      philip.koeck-at-csb.ki.se
Date: Mon, 04 Dec 2000 16:44:36 +0100
Subject: TEM resolution

Contents Retrieved from Microscopy Listserver Archives
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Hallo everybody,

I've started wondering about what actually the point resolution of
a TEM in phase-contrast imaging is.

} From Raleigh's criterion I would conclude that for a typical 120 kV
TEM with a numerical aperture of about 20 mrad distances larger than
about 2 Angstrom or 'density waves' with a wavelength longer than
2 Angstrom simply cannot be resolved.

In Scherzer defocus the Phase Contrast Transfer Function (PCTF) has its
first zero at about the same resolution but there seems to be
information
at higher frequencies (even if it is inverted in contrast).

Which is the true resolution limit?

Yours sincerely,

Philip

--
Philip Koeck
Karolinska Institutet
Dept. of Bioscience
Novum
S-14157 Huddinge
Sweden
Tel.: +46-8-608 91 86
Fax.: +46-8-608 92 90
Email: Philip.Koeck-at-csb.ki.se
http://www.csb.ki.se/em


From daemon Mon Dec 4 10:49:11 2000



From: Ann Dvorak :      advorak-at-caregroup.harvard.edu
Date: Mon, 4 Dec 2000 11:55:34 -0500
Subject: tricks for better ribbons?

Contents Retrieved from Microscopy Listserver Archives
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Trying to cut serial sections of Spurr for 3D reconstruction. Anyone know
of any tricks to make the sections stay together in long ribbons? Ellen
Morgan


From daemon Mon Dec 4 13:28:57 2000



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Mon, 04 Dec 2000 11:26:15 -0800
Subject: Re: TEM resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Philip,

In the field of high-resolution transmission electron microscopy, the
"resolution" is defined as the first zero in the phase contrast transfer
function (PCTF) at Scherzer (or optimum) defocus as you point out. This
definition is chosen because any higher-frequency information is phase
inverted (as you also point out). However, it results in defining the
resolution in terms of the spatial frequency at which transfer goes to zero,
rather than in terms of the highest transferred spatial frequency.

I have suggested a definition in which the resolution is defined at the 70%
pass limit within the Scherzer passband, giving d = 0.67 Cs^1/4 lambda^3/4
at an "extended" optimum defocus of sqrt(1.5Cs.lambda) instead of Scherzer's
d = 0.707 Cs^1/4 lambda^3/4 at an optimum defocus of sqrt(Cs.lambda).
Current practice seems to use the extended defocus with the crossover
(zero-transfer) frequency to get d = 0.64 Cs^1/4 lambda^3/4 at the
"extended" optimum defocus.

The information beyond the Scherzer cross-over (zero-transfer) frequency can
be utilized to provide structural information. Because of the misphasings,
this information is difficult to interpret directly. Originally, the way to
get at this higher-frequency (smaller-spacing) information was to compare
focal series of experimental images with images simulated from possible
models. In this way, we "saw" the small tunnels in Nb12O29 (with a
2.5Angstrom spacing) with a JEOL 100B (Scherzer resolution of 3.5A) in a
focal series obtained by Sumio Iijima (see "Resolution-limiting effects in
electron microscope images", G.R. Anstis and M.A. O'Keefe, In 34th Ann.
Proc. EMSA, Miami Beach (1976) 480-481). I used HRTEM image simulation
programs such as my SHRLI (simulated high-resolution lattice image) code, or
my later interactive TEMPaS (TEM processing and simulation) code (ported to
the Mac as MacTempas) with great success, but only when the model was known
or limited to several possible ones (for example "Inversion domains in GaN
grown on sapphire", L.T. Romano, J.E. Northrup and M.A. O'Keefe, Appl. Phys.
Lett. 69 (1996) 2394-2396). Currently, post-Scherzer information can be
accessed by image reconstruction algorithms from focal series, or tilt
series, or holography. I use the Philips/Brite-Euram software for
focal-series reconstruction by Coene and Thust in my one-Angstrom microscope
(OAM) project to extend the resolution of a modified Philips CM300FEG/UT
from its native resolution of 1.7Angstrom to a super-resolution of
0.89Angstrom that can image the "dumbbells" in [110] diamond (see “The NCEM
One-Ångstrom Microscope project reaches 0.89Å resolution”, M. A. O'Keefe in
58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000) 1192-1193).

For the equations that belong with a discussion of resolution, see
"Resolution in high-resolution electron microscopy", M.A. O'Keefe,
Ultramicroscopy 47 (1992) 282-297. For a (simple) explanation of the use of
focal-series to obtain super-resolution see "Push TEM limits with
super-resolution", Michael A. O'Keefe (1999), R&D Magazine October 1999, p79
or {http://www.rdmag.com/archives/basics/10microscopy.htm} . For details of
the Philips/Brite-Euram software for focal-series reconstruction by Coene
and Thust, see W.M.J. Coene, A. Thust, M. Op de Beeck and D. Van Dyck,
Ultramicroscopy 64 (1996) 109-135 and A. Thust, W.M.J. Coene, M. Op de Beeck
and D. Van Dyck, Ultramicroscopy 64 (1996) 211-230.
In the above, I have dealt only with linear terms in the transfer of
information from the specimen (more properly, the exit-surface wave from the
specimen) into the HREM image. Non-linear terms can give rise to
even-higher spatial frequencies in the image. However, the question of
whether these can be termed "resolution" is doubtful (see
"Resolution-damping functions in non-linear images", M.A. O'Keefe, in 37th
Ann. Proc. EMSA, San Antonio, Texas (1979) 556-557).

Mike O'Keefe


Philip Koeck wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hallo everybody,
}
} I've started wondering about what actually the point resolution of
} a TEM in phase-contrast imaging is.
}
} } From Raleigh's criterion I would conclude that for a typical 120 kV
} TEM with a numerical aperture of about 20 mrad distances larger than
} about 2 Angstrom or 'density waves' with a wavelength longer than
} 2 Angstrom simply cannot be resolved.
}
} In Scherzer defocus the Phase Contrast Transfer Function (PCTF) has its
} first zero at about the same resolution but there seems to be
} information
} at higher frequencies (even if it is inverted in contrast).
}
} Which is the true resolution limit?
}
} Yours sincerely,
}
} Philip
}
} --
} Philip Koeck
} Karolinska Institutet
} Dept. of Bioscience
} Novum
} S-14157 Huddinge
} Sweden
} Tel.: +46-8-608 91 86
} Fax.: +46-8-608 92 90
} Email: Philip.Koeck-at-csb.ki.se
} http://www.csb.ki.se/em

--------------------------------------------------------
Your mail has been rejected for the following reason(s):
--------------------------------------------------------
OK, Nestor -- this time it's in plain text.
-Mike



From daemon Mon Dec 4 14:24:14 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 4 Dec 2000 15:23:19 -0500
Subject: Re: tricks for better ribbons?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:55 AM -0500 12/4/00, Ann Dvorak wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

************************
Hi Ellen,
I vaguely remember reading something about coating the sides of the
block that will become the leading and trailing faces with something
slightly tacky (like the adhesive dissolved off of 3M Magic Scotch
tape). Once that has dried, the edges of the sections are supposed
to stick together. I've never actually tried it, and I wonder how
you do manage to separate the sections where you need to.

Does anyone out there remember this? Has anyone tried it?

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Dec 4 14:36:31 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 4 Dec 2000 14:33:04 -0600
Subject: MSA Archivist position

Contents Retrieved from Microscopy Listserver Archives
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The Microscopy Society of America is seeking an Archivist to manage
the historical documents of our Society. This position is appointed
by MSA Council for a period of three years.

We are looking for someone who is experienced in the storage and
cataloging of (primarily) paper documents. Although this is a
voluntary position (non-paying), the Society would provide funding
for expendibles and day-to-day operations.

If interested (or if you know of a qualified person), please contact me at:

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Mon Dec 4 14:49:05 2000



From: Schumacher, Elaine :      efschuma-at-uop.com
Date: Mon, 4 Dec 2000 14:45:42 -0600
Subject: RE: Tricks for Better Ribbons

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

A predecessor in our laboratory came back from the MSA conference a few
years back with a sample from a vendor of something called Tacki Wax, which
was supposed to serve this purpose. I think the idea was that the sections
would be gently held together, but not so strongly that you couldn't work
them apart as needed while collecting them. I've never tried it myself, as
I don't need to do serial sectioning, but having a product name to go by
might help your search.

Elaine Schumacher
UOP
25 East Algonquin Rd.
Des Plaines, IL 60017-5017
phone: 847-391-3403
fax: 847-391-3719
efschuma-at-uop.com


From daemon Mon Dec 4 15:42:46 2000



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 4 Dec 2000 14:18:55 -0600
Subject: Information about the 2001 UBC "Live Cell" course

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

I am pleased to announce that the Live-cell Course continues to grow
and prosper. Over 130 students from 23 countries have taken the
course over the last 5 years.

Now I am organizing the next 11-day summer course on "3D Microscopy
of Living Cells" for June of 2001. As in the past it will be at the
University of British Columbia, in Vancouver, BC. The Fifth Workshop
on 3D Image Processing will be held June 30 - July 2.

Please check out http://www.cs.ubc.ca/spider/ladic/course/bulletin.html
if you would like to have a feeling for last years's course.

The information for 2001 is below.

Thanks for your help,

Jim Pawley


Sixth Annual INTERNATIONAL 11-Day Short Course on

3D Microscopy of Living Cells
June 18 - 28, 2001



Fifth, Post-course Workshop on

3D Image Processing,
June 30 - July 2, 2001



Organized by Prof. James Pawley,
(University of Wisconsin-Madison)
(SEE ADDRESS AT END OF MESSAGE)


in association with Dr. Elaine Humphrey
UBC BioSciences Microscopy Facility:
University of British Columbia
Vancouver, BC, Canada


DATES

Applications must be received by March 1, 2001
Deposit due April 15, 2001
Registration 5:00 - 7:00 PM Sunday, June 17, 2001
First Lecture 7:30 PM Sunday, June 17, 2001
Live-cell Course ends, noon Thursday, June 28, 2001
3D Image Processing Course, June 30, - July 2, 2001


APPLICATIONS DUE BY MARCH 1, 2001


APPLICATIONS
Applicants must complete a questionnaire to assess knowledge level,
field of interest and proposed personal, live-cell, project.
Enrollment will be limited to about 24 participants (exact number
depends on number of 3D Systems available). Selection will be made
on the basis of background and perceived need. Those without
previous LM experience will be provided with access to basic texts to
read before the course begins. Application forms requesting
information on field of interest and level of experience may be
down-loaded from the WWW site at
http://www.cs.ubc.ca/spider/ladic/course/reg1.htm, or obtained from:

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4

Additional information is available from:
http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

We expect to have at least 11, 3D microscope workstations for student
use and there will be an international faculty of 17.

Application deadlines:

Application forms must be received for screening by March 1, 2000.
Successful applicants will be notified by April 1, and a deposit of
50% must be received by April 15, 2000. In general, refunds of the
deposit will not be possible. The remainder is due before
Registration.


3D Course tuition (includes lunches and snacks): $2150 (US)
Workshop Tuition (includes lunches and snacks): $850 (US)

Room / board about $40/day (US)

I hope that this includes all of the information that you need, but
if not, please get back to me.
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Mon Dec 4 16:48:33 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Mon, 4 Dec 2000 17:41:59 -0500 (EST)
Subject: Help with 3d software

Contents Retrieved from Microscopy Listserver Archives
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Mike,

Thanks for offering to put my 3D rendering question on the
discussion group.
My lab currently acquires and processes images using IPlab and a
SPOT RT camera. We have MicroTome by VayTek for deconvolution, but this
isn't much good without 3D rendering. Our primary application will be
measuring the volume of nuclei and cells which have been fluorescently
stained. Which 3D programs for a PC might work well for this?

Thanks,
Darren







From daemon Mon Dec 4 16:51:24 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Mon, 4 Dec 2000 17:48:00 -0500 (EST)
Subject: chemlumanesence

Contents Retrieved from Microscopy Listserver Archives
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Hello all,
Any one with on advice on who in the Baltimore area can do
chemlumanesence (spelling?) on live cells, realtime and time series?
What equipment do we need, which company or labs are doing this.

thanks,
Mike D.



From daemon Mon Dec 4 17:14:58 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 4 Dec 2000 16:08:10 -0700
Subject: TEM resolution

Contents Retrieved from Microscopy Listserver Archives
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OK,

I'll take a stab at this. Please correct me if I am wrong:

The point resolution refers to the resolution limit of a TEM for (as the
name implies) two point like objects. A pointlike object is mathematically a
delta-function and has a Fourier spectrum that is constant for all
frequencies. So the question becomes: how many of the Fourier components can
be transmitted without changing them, which in turn determines the minimum
size of the object. The answer is then basically given by the first zero of
the CTF. Until this point, the spatial frequencies are transmitted with the
same phase and almost the same amplitude. For higher frequencies, the
amplitude of the CTF oscillates, so that no reliable transmission can be
achieved. The setting, where the first zero is furthest out in Fourier space
is the Scherzer defocus. Thus the point resolution is determined by the
first zero of the CTF at Scherzer defocus.

Line resolution refers to a periodic structure with very few Fourier
components. It is therefore not necessary to transmit the entire Fourier
spectrum, but only one specific component. Thus by changing the CTF through
changing the focus, one can find a setting, where that specific frequency is
transmitted and shows up in the image. The question therefore becomes: How
well can I detect the structure. This is then in principle determined by the
amplitude of the CTF. So the line resolution is then determined by the CTF
envelope going below a certain threshold (I believe it is 20%, but I am not
sure). That threshold is of course more or less arbitrary, but should give a
reasonable estimate of the line resolution and allow comparison of the
microscope resolutions.

Disclaimer: Please use this with caution. I have not been involved with EM
theory for a few years. For a good account of high-resolution TEM, check out
the book(s) by John Spence.

Michael Bode


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Philip Koeck [mailto:philip.koeck-at-csb.ki.se]
Sent: Monday, December 04, 2000 8:45 AM
To: 3dem list; MSA mailing list


Hallo everybody,

I've started wondering about what actually the point resolution of
a TEM in phase-contrast imaging is.

} From Raleigh's criterion I would conclude that for a typical 120 kV
TEM with a numerical aperture of about 20 mrad distances larger than
about 2 Angstrom or 'density waves' with a wavelength longer than
2 Angstrom simply cannot be resolved.

In Scherzer defocus the Phase Contrast Transfer Function (PCTF) has its
first zero at about the same resolution but there seems to be
information
at higher frequencies (even if it is inverted in contrast).

Which is the true resolution limit?

Yours sincerely,

Philip

--
Philip Koeck
Karolinska Institutet
Dept. of Bioscience
Novum
S-14157 Huddinge
Sweden
Tel.: +46-8-608 91 86
Fax.: +46-8-608 92 90
Email: Philip.Koeck-at-csb.ki.se
http://www.csb.ki.se/em


From daemon Mon Dec 4 17:16:29 2000



From: Jon Ekman :      jekman-at-csd.uwm.edu
Date: Mon, 4 Dec 2000 17:14:59 -0600
Subject: Re: tricks for better ribbons?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,


For Light microscopy, we used a mixture of Weldwood contact
cement from the local hardware store and toluene mixed 50/50. Place
it on the leading and trailing edges of the block using a pin. We used
a tool to pick up the sections I don't remember its name. It looked
like a small metal trough.


For EM I used to make the block face area as small as possible
focusing on a specific area of interest. Then I would cut the the
leading and trailing edges parallel and then put the block on the ultra
microtome and polish the edges with a glass knife set on zero
advance. If the leading and trailing edges are smooth the sections
stick together when coming off the block. I would make groups of
ten sections. pick up with a tungsten wire loop (premade loops work
better from EM venders of your choice) and place on a formvar
coated slot grid.


Good Luck


Jon








{color} {param} 0100,0100,0100 {/param} On 4 Dec 00, at 15:23, Leona Cohen-Gould wrote:


{color} {param} 7F00,0000,0000 {/param} } ----------------------------------------------------------------------

} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of

} America To Subscribe/Unsubscribe -- Send Email to

} ListServer-at-MSA.Microscopy.Com On-Line Help

} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} ----------------------------------------------------------------------

} -.

}

}

} At 11:55 AM -0500 12/4/00, Ann Dvorak wrote:

} } ---------------------------------------------------------------------

} } --- The Microscopy ListServer -- Sponsor: The Microscopy Society of

} } America To Subscribe/Unsubscribe -- Send Email to

} } ListServer-at-MSA.Microscopy.Com On-Line Help

} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} } ---------------------------------------------------------------------

} } --.

} }

} }

} } Trying to cut serial sections of Spurr for 3D reconstruction. Anyone

} } know of any tricks to make the sections stay together in long

} } ribbons? Ellen Morgan

}

} ************************

} Hi Ellen,

} I vaguely remember reading something about coating the sides of the

} block that will become the leading and trailing faces with something

} slightly tacky (like the adhesive dissolved off of 3M Magic Scotch

} tape). Once that has dried, the edges of the sections are supposed to

} stick together. I've never actually tried it, and I wonder how you do

} manage to separate the sections where you need to.

}

} Does anyone out there remember this? Has anyone tried it?

}

} Lee

}

} Leona Cohen-Gould, M.S.

} Sr. Staff Associate

} Director, Electron Microscopy Core Facility

} Manager, Optical Microscopy Core Facility

} Joan & Sanford I. Weill Medical College

} of Cornell University

} voice (212)746-6146

} fax (212)746-8175

}

}



{nofill}
Jon Ekman
Associate Research Specialist
Deptartment of Biological Sciences
University of Wisconsin-Milwaukee
phone W:414.229.6471
Web1 http://www.graffitimasters.com
Web2 http://www.uwm.edu/~jekman


From daemon Mon Dec 4 17:54:48 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 4 Dec 2000 18:49:23 -0500
Subject: profiler images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to know whether there are any commercial labs that can provide
up to 3000 um x 3000 um contact profilometry image maps such as the Veeco
Dektak Profiler or the KLA-Tencor Profiler can provide.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Mon Dec 4 18:19:27 2000



From: Linda Barthel :      barthel-at-umich.edu
Date: Mon, 4 Dec 2000 18:14:59 -0600
Subject: Serial sections.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Go to Rivlin and Raymond, 1987, Journal of Neuroscience Methods, 20:23-33,
also see Raymond and Rivlin, 1987, Developmental Biology, 122:120-138.
There you will find reference to the technique originally published by W.
Fahrenbach, 1984, J. Electron. Microsc. Tech., 1:387-398. In the Rivlin
paper you will find that a solution of Weldwood cement (rubber cement) was
applied to the top and bottom edges of the trapezoid block face. This glue
can be use undiluted or diluted in the appropriate solvent to obtain the
desired workable concentration.

Linda Barthel, M.S.
Research Associate II
University of Michigan
Department of Cell and Developmental Biology
4607 Medical Science Building II
Ann Arbor, MI 48109-0616

(734) 764-7476

http://www-personal.umich.edu/~praymond




From daemon Mon Dec 4 20:26:38 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 4 Dec 2000 21:19:58 EST
Subject: wanted books and stuff on science fighting crime, American Journal of Police Sc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looking for back issues or bound volumes of
American Journal of Police Science, edited by
the Scientific Crime Detection Laboratory,

Also will purchase books showing how to use
bullet comparison microscopes, finger print cameras,
hair and fiber comparison microscopes, etc., etc.

Think of it this way, if it is using science, medicine and gadgets to solve
crime we need artifacts,ephemera and books for our museum display.

Will pay cash or trade goodies!

Thanks Ed Sharpe, Archivist for SMECC


From daemon Mon Dec 4 23:58:46 2000



From: charles j day :      wa5ekh-at-juno.com
Date: Sat, 2 Dec 2000 23:49:53 -0500
Subject: Polymers TEM/SEM position in Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There may be a position opening up in the Dallas/Ft. Worth Area for an
experienced microscopist with polymers-TEM experience. If you might be
interested please send me a resume.
Jeff Day/wa5ekh-at-juno.com
________________________________________________________________
GET INTERNET ACCESS FROM JUNO!
Juno offers FREE or PREMIUM Internet access for less!
Join Juno today! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.


From daemon Tue Dec 5 08:34:46 2000



From: Ed Kurz :      ekurz-at-mail.ims.uconn.edu
Date: Tue, 05 Dec 2000 09:31:52 -0500
Subject: Wafer Cross sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have suggestions as to how to polish silicon wafers in cross
section. I have a wafer (~0.5mm thick ) which has been randomly fractured
with about a micron of metal on the surface and want to determine the metal
thickness. We are trying various methods - first choice is by RBS but
presently limited in max. energy so can not penetrate layer.

In my first couple of attempts at polishing (embedded wafer in thermoset)
the silicon breaks quite easily producing a very rough surface. I assume I
am being too aggressive and am trying slower more gentle procedure. Does
anyone have a procedure for polishing cross sections of such brittle material?

Thanks,
Ed


From daemon Tue Dec 5 10:16:16 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Tue, 5 Dec 2000 09:47:29 -0500
Subject: SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following comments are for the person who is trying to cut serial
sections with Spurrs. I routinely cut serial sections with Spurrs without
resorting to any kind of glue. Probably the most important thing is how the
block is faced. I cut the trapezoid with a really long (.6 to .9 mm base)
and the top of the trapezoid is almost the same length. What's really
critical here is that those two cuts are parallel.
The two sides of the trapezoid are very short (.1mm). I cut the sections
with a Diatome knife and on a Reichert Ultracut E with a cutting speed of
0.8mm per second and a thickness setting of 80nm. I am doing this in a
small room with a blasting air vent in the ceiling. I have cut up a
cardboard box and stuck it into the ceiling tiles in such a way that the air
is diverted away from the microtome. I can pick up 15 to 25 sections in a
row on a 0.4 X 2mm copper slotted grid coated with just formvar or
formvar/carbon. I have never been any good at making the support films my
self, so I buy the slotted grids precoated from Ted Pella (catalog #s 01706,
01806, or 01816). Before picking up the ribbon, I dip a stick into
chloroform and wave that over the sections, and they expand (or relax,
depending on your point of view). I have a bunch of self locking tweezers
and after picking up the sections, I leave the grid with the sections on it
locked in the tweezers until they dry (if you put a wet slotted grid down on
filter paper it might break the film). Good luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Tue Dec 5 10:43:18 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 4 Dec 2000 17:06:29 -0800
Subject: Re: tricks for better ribbons?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Trying to cut serial sections of Spurr for 3D reconstruction. Anyone know
} of any tricks to make the sections stay together in long ribbons? Ellen
} Morgan

Ellen -

I assume that you're talking about EM. not LM. Make sure that the top and
bottom of your block face are REALLY smooth and clean - and as small as is
consistent with the reconstruction. Viewed thru the microtome binocular,
they should look glassy, not like sandpaper. Change your trimming tool
(glass knife? Weck razor blade?) often.

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Dec 5 11:22:55 2000



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Tue, 5 Dec 2000 17:13:27 -0000
Subject: HP LJ IIIp - EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Has anyone used a HP Laserjet IIIp with an EDAX PV9900, or know what drivers
are needed, or where we can acquire them?

We are trying to use the IIIp because it has serial and parallel connections
needed by the EDAX and by the Philips XL40 SEM with which we use it and we
cant afford to buy a new one.

Reply in confidence to chris.smith-at-bbsrc.ac.uk if you don't want to clog up
the list.

Many thanks, Chris, Plant Path., IACR-Rothamsted, UK.


From daemon Tue Dec 5 12:46:27 2000



From: Edward Hirsch :      edhirsch-at-att.net
Date: Tue, 5 Dec 2000 13:38:12 -0600
Subject: Wafer Cross sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ed,
I represent Allied High Tech Products. We have detailed procedures for
preparing semiconductor device cross-sections. We also offer all the
necessary products to accomplish it. I would be happy to speak to you and
provide you with detailed procedures on how to prepare these samples. Since
it can be quite detailed it would be best to do this offline. Please
provide me with your phone number and I will call you.

Sincerely,

Ed

*************************************************
Edward A. Hirsch
Product Application Specialist
Allied High Tech Products
2376 East Pacifica Place
Rancho Dominguez, CA 90220
ph: (919) 846-9628
vm:(800)675-1118 x245
fx: (310)762-6808
http://www.alliedhightech.com

Equipment and Consumables for Metallurgical Sample Preparation
*************************************************



-----Original Message-----
} From: Ed Kurz [mailto:ekurz-at-mail.ims.uconn.edu]
Sent: Tuesday, December 05, 2000 8:32 AM
To: MICROSCOPY BB


Does anyone have suggestions as to how to polish silicon wafers in cross
section. I have a wafer (~0.5mm thick ) which has been randomly fractured
with about a micron of metal on the surface and want to determine the metal
thickness. We are trying various methods - first choice is by RBS but
presently limited in max. energy so can not penetrate layer.

In my first couple of attempts at polishing (embedded wafer in thermoset)
the silicon breaks quite easily producing a very rough surface. I assume I
am being too aggressive and am trying slower more gentle procedure. Does
anyone have a procedure for polishing cross sections of such brittle
material?

Thanks,
Ed



From daemon Tue Dec 5 13:57:36 2000



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Tue, 5 Dec 2000 13:54:19 -0600
Subject: Value of used EDS detector?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a two year old PGT Prism EDS detector/preamp/dewar assembly which we
are thinking of selling. But I don't have much of an idea of the value of
such a device.

Any thoughts?

Anyone interested?

Richard Shalvoy
Arch Chemicals
Cheshire, CT
203-271-4394


From daemon Tue Dec 5 13:57:41 2000



From: Nafisa Ghori :      nghori-at-stanford.edu
Date: Tue, 05 Dec 2000 11:52:02 -0800
Subject: Negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all ,
Does any body use Ammonium Molybdate for Negative staining and also by this
chemical visualize structure better then Uranyl acetate.


From daemon Tue Dec 5 19:25:57 2000



From: Koh YinHsian-CYK006 :      Yinhsian.Koh-at-Motorola.com
Date: Wed, 6 Dec 2000 09:13:53 +0800
Subject: Polymers TEM/SEM position in Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry Jeff,

This is not a job application. Would like to seek your advice on how to analyse a polymer samples using TEM. We have tried on Poly-carbonate injection molded lense with hard coating. The purpose is to see the interface bonding of the hard coat on the lense. Unfortunately, when we microtome the samples, the sample curve up and we cannot manage to see anything. Would appreciate if you can share your experience on the sample preparation with us.

Thanks in advance of your help.

Regards,
YH Koh
(MQE Engineer)
Motorola CGISS Penang,
Bayan Lepas FIZ, Phase 3,
11900 Penang, Malaysia.
Tel : 60-4-8504089
Fax: 60-4-6124903


-----Original Message-----
} From: charles j day [mailto:wa5ekh-at-juno.com]
Sent: Sunday, December 03, 2000 12:50 PM
To: Microscopy-at-sparc5.microscopy.com


There may be a position opening up in the Dallas/Ft. Worth Area for an
experienced microscopist with polymers-TEM experience. If you might be
interested please send me a resume.
Jeff Day/wa5ekh-at-juno.com
________________________________________________________________
GET INTERNET ACCESS FROM JUNO!
Juno offers FREE or PREMIUM Internet access for less!
Join Juno today! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.


From daemon Tue Dec 5 19:54:04 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Tue, 5 Dec 2000 19:51:05 -0600
Subject: Fw: Wavefront Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


forwarded from the imaging news group

"james patrick birrell" {jbirrell-at-students.uiuc.edu} wrote in message
news: {Pine.GSO.4.10.10012041635310.22505-100000-at-ux12.cso.uiuc.edu} ...
} I was wondering if anybody would know where I could obtain software to
} facilitate the acquisition/analysis of images to be used to perform
} wavefront reconstruction using images from a TEM. Specifically, I'm
} looking for any software compatable with a JEOL 4000ex microscope with a
} Gatan CCD camera.
} Thanks,
} James Birrell




From daemon Tue Dec 5 21:45:09 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 05 Dec 2000 19:43:40 -0800
Subject: Re: Negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ammonium molybdate (AM) is suitable in the cases when uranyl acetate (UA)
is bad (mostly if pH is a critical factor, UA pH = 5-5.5). In most cases AM
gives less contrast than UA. AM gives you "pure" negative staining because
do not chemically interfere with most biological samples. UA from another
hand sometimes gives you mixed contrast, positive
(DNA/RNA)&negative(proteins). For instance, ribosomes and many viruses
shown mixed staining with UA. It's not bad because it may even enhance
some structural details, but you have to remember about that. My own
experience indicated that in most cases UA or other uranium salts
(U-formiate or oxalate) after conditions adjusting (concentration, time,
temperature, freshness, support film) has shown perfect results. If you
have a problem with stain spreading, you may try different support films
(carbon, formvar, parlodion). Very good results you may obtain using
"double carbon" technique - this may solv even very worse cases. I also
has have a good results with poly-lysine treatment of the grids prior
sample adsorption. Personally, I don't like the glow-discharge. But it
works in some way.

Sergey.

At 11:52 AM 12/5/00 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Dec 5 21:47:45 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Tuesday, December 05, 2000 2:11 PM
Subject: HP LJ IIIp - EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chris,

EDAX has the HP laser printer driver for old DEC based systems. Call EDAX,
or www.edax.com

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: chris smith (IACR-RES) {chris.smith-at-bbsrc.ac.uk}
To: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}




From daemon Wed Dec 6 02:14:07 2000



From: Bart De Pauw :      Bart.DePauw-at-rug.ac.be
Date: Wed, 06 Dec 2000 09:10:17 +0100
Subject: SEM - Choice of buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listers,

We use cacodylate buffer for our SEM samples. Is HEPES or PIPES buffer
an alternative for the toxic cacodylate ? Our samples are mainly animal
tissue.


Bart De Pauw
Ghent University
Faculty of Veterinary Medicine
Department Morphology
Belgium



From daemon Wed Dec 6 03:04:03 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 6 Dec 2000 09:01:12 +0000 (GMT Standard Time)
Subject: Re: SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I found your post interesting. How do you make your edges
parallel? Do you use a razor blade?

Dave



On Tue, 5 Dec 2000 09:47:29 -0500 Timothy Schneider
{Timothy.Schneider-at-Mail.TJU.EDU} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The following comments are for the person who is trying to cut serial
} sections with Spurrs. I routinely cut serial sections with Spurrs without
} resorting to any kind of glue. Probably the most important thing is how the
} block is faced. I cut the trapezoid with a really long (.6 to .9 mm base)
} and the top of the trapezoid is almost the same length. What's really
} critical here is that those two cuts are parallel.
} The two sides of the trapezoid are very short (.1mm). I cut the sections
} with a Diatome knife and on a Reichert Ultracut E with a cutting speed of
} 0.8mm per second and a thickness setting of 80nm. I am doing this in a
} small room with a blasting air vent in the ceiling. I have cut up a
} cardboard box and stuck it into the ceiling tiles in such a way that the air
} is diverted away from the microtome. I can pick up 15 to 25 sections in a
} row on a 0.4 X 2mm copper slotted grid coated with just formvar or
} formvar/carbon. I have never been any good at making the support films my
} self, so I buy the slotted grids precoated from Ted Pella (catalog #s 01706,
} 01806, or 01816). Before picking up the ribbon, I dip a stick into
} chloroform and wave that over the sections, and they expand (or relax,
} depending on your point of view). I have a bunch of self locking tweezers
} and after picking up the sections, I leave the grid with the sections on it
} locked in the tweezers until they dry (if you put a wet slotted grid down on
} filter paper it might break the film). Good luck, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Dec 6 03:43:39 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Wed, 6 Dec 2000 09:40:12 +0000 (GMT Standard Time)
Subject: RE: Polymers TEM/SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Wed, 6 Dec 2000, Koh YinHsian-CYK006 wrote:

{ { Would like to seek your advice on how to analyse a polymer sample using
TEM. We have tried on Poly-carbonate injection molded lens with hard
coating. The purpose is to see the interface bonding of the hard coat on
the lens. Unfortunately, when we microtome the samples, the sample curves
up and we cannot manage to see anything. Would appreciate if you can share
your experience on the sample preparation with us. } }

We have looked at surface layers in polymer samples by cutting open a
surface, then etching and looking, NOT at a cut section, but at the
cross-sectional view of the remaining surface, either by SEM or by
replication/TEM. Usually we etch our specimens, so before cutting open, a
protective layer is applied over the outer surface to prevent this being
etched downwards. Mainly we have looked at polyethylene or polypropylene
specimens, using our permanganic etching technique, and different reagents
would be needed for polycarbonate. However, it might be possible to
replicate the cut surface directly and get useful information.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+




From daemon Wed Dec 6 08:39:14 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Wed, 6 Dec 2000 09:24:49 -0500
Subject: MORE SERIAL SECTIONS

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The question was: how do I make the top and bottom edges parallel? I do it
by eye. I use Gem single edge industrial blades from Ted Pella Cat# 121-1
(I'm sure these are available from many sources). One important point is
that I spray them with 70% ETOH before using and wipe them off with a
Kimwipe EXL. A word about the way I described the trapezoid face: it
doesn't look like a trapezoid. What it looks like is a very thin shiny line
(heck, I'm talking about putting 15 to 25 of these guys on a 2mm slot, you
do the math). More good luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Wed Dec 6 08:58:56 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 6 Dec 2000 10:54:31 -0400
Subject: subscribe

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From daemon Wed Dec 6 09:23:04 2000



From: carla_aiwohi-at-usgs.gov
Date: Wed, 6 Dec 2000 07:19:13 -0800
Subject: thanks-SEM conductive adhesive help

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Thanks to all who responded to my question regarding conductive adhesives
for salmon skin.
I have ordered many of the products that were suggested and am looking
forward to success (finally) :)

Thanks again and have a great holiday season!


Carla Aiwohi
Western Fisheries Research Center
Seattle, WA



From daemon Wed Dec 6 09:55:48 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 6 Dec 2000 09:34:38 -0600
Subject: Re: SEM - Choice of buffer

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We routinely use HEPES for our fixations with paraformaldehyde,
glutaraldehyde and osmium. We see little or no difference from
classical cacodylate buffers. I chose HEPES a long time ago and in
retrospect, PIPES might have been a slightly better choice since
HEPES has a pKa of 7.5 and PIPES has a pKa of 6.76. Since one
generally uses aldehyde buffers at pH 7.0 to 7.4, either would seem
appropriate but since I read that during fixation, H+ ions are
generated and the solution tends to acidify, then having a buffer on
the high side of the pKa would be better since you would have greater
buffering power. Despite this "improved" logic, I have remained
loyal to HEPES and never had a problem. Good luck.



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From daemon Wed Dec 6 10:49:13 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 6 Dec 2000 11:44:32 -0500 (EST)
Subject: Gurr buffer recipe?

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Would anyone happen to have the actual recipe for Gurr's buffer? I think
it is some phosphate formulation - we've been hunting the recipe and have
found some refs, but will have to interlibrary the journals/books and we
thought we'd give the 'net a try, first.

I know we can buy the pre-made buffer, but we need such a tiny amount that
we'd rather make it from scratch (plus we want to know what it is!).

Thanks!

Tamara Howard
Cold Spring Harbor Laboratory, NY



From daemon Wed Dec 6 11:13:57 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 06 Dec 2000 17:20:07 +0000
Subject: Re: SERIAL SECTIONS

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Timothy

I would be a bit concerned about your use of chloroform to flatten sections,
especially in a small microtome room. We have used heat pens for the last decade
or so, because apparently the chloroform we use these days is a lot more toxic
than it used to be (as well as formaldehyde, glutaraldehyde, carbon
tetrachloride etc).

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Timothy Schneider wrote:

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} -----------------------------------------------------------------------.
}
} The following comments are for the person who is trying to cut serial
} sections with Spurrs. I routinely cut serial sections with Spurrs without
} resorting to any kind of glue. Probably the most important thing is how the
} block is faced. I cut the trapezoid with a really long (.6 to .9 mm base)
} and the top of the trapezoid is almost the same length. What's really
} critical here is that those two cuts are parallel.
} The two sides of the trapezoid are very short (.1mm). I cut the sections
} with a Diatome knife and on a Reichert Ultracut E with a cutting speed of
} 0.8mm per second and a thickness setting of 80nm. I am doing this in a
} small room with a blasting air vent in the ceiling. I have cut up a
} cardboard box and stuck it into the ceiling tiles in such a way that the air
} is diverted away from the microtome. I can pick up 15 to 25 sections in a
} row on a 0.4 X 2mm copper slotted grid coated with just formvar or
} formvar/carbon. I have never been any good at making the support films my
} self, so I buy the slotted grids precoated from Ted Pella (catalog #s 01706,
} 01806, or 01816). Before picking up the ribbon, I dip a stick into
} chloroform and wave that over the sections, and they expand (or relax,
} depending on your point of view). I have a bunch of self locking tweezers
} and after picking up the sections, I leave the grid with the sections on it
} locked in the tweezers until they dry (if you put a wet slotted grid down on
} filter paper it might break the film). Good luck, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu



From daemon Wed Dec 6 11:44:29 2000



From: m.andersson-at-t-online.de (Maike Andersson)
Date: Wed, 6 Dec 2000 18:48:01 +0100
Subject: Need help on staining semithin sections embedded in LR-white

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LM - Need help on staining semithin sections embedded in LR-white

I am doing anatomical studies on the seed coat and currently use toluidine
blue
with sodium hypochlorite prestaining and iodine/potassium iodite
poststaining, described by
Gutmann, M. : Improved staining procedures for photografic documentation of
phenolic deposits in semithin sections of plant tissue. Journal of
Microscopy 179:277-281 (1995)
Can anyone suggest a better stain, specially one that is suitable for
staining lignin ?



From daemon Wed Dec 6 13:27:07 2000



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Wed, 06 Dec 2000 11:19:42 -0800
Subject: Ammonium molybdate

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I have used 1% ammonium molybdate, pH7.3 -at-300milliosmoles for negative staining of liposomes. It worked better than UA.

Barbara L. Plowman
University of the Pacific
School of Dentistry
San Francisco, CA
phone: 415-929-6692
email: Bplowman-at-sfuop.edu



From daemon Wed Dec 6 13:27:12 2000



From: Zhenquan Liu :      zhenquan.liu-at-asu.edu
Date: Wed, 06 Dec 2000 12:21:15 -0700
Subject: Re: Wafer Cross sections

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Hi, Ed,

I think you may try the following way:

1. Apply a thin layer of M-bond on the surfaces of two pieces of your wafer
and put these two surfaces together.
2. Use a clip to press these pieces of wafers as close as possible (not to
break them).
3. Leave the sample to try (24 hours in air? or 1 hour at 120 C degree on a
hot plate).

This is a good way to protect the thin "film" (your metal features) I
think. The smaller the gap between these two pieces, the better. In this
way you can get two cross section surfaces. Even after step 3, you may
apply another thin layer of M-bond on the outside surfaces of the sample,
so that you may get 4 surfaces for examination.

4 Embed the sample in a suitable epoxy as you did before, and grinding and
polishing the cross sections carefully from coarse to fine.

When you observe the cross sections, it is easy to see what is the M-bond
and what is the other thing you want to see.

Just a suggestion.

Good luck!

Zhenquan Liu


I think you may try the following way:

1. Apply a thin layer of M-bond on the surfaces of two pieces of your wafer
and put these two surfaces together.
2. Use a clip to press these pieces of wafers as close as possible (not to
break them).
3. Leave the sample to try (24 hours in air? or 1 hour at 120 C degree on a
hot plate).

This is a good way to protect the thin "film" (your metal features) I
think. The smaller the gap between these two pieces, the better. In this
way you can get two cross section surfaces. Even after step 3, you may
apply another thin layer of M-bond on the outside surfaces of the sample,
so that you may get 4 surfaces for examination.

4 Embed the sample in a suitable epoxy as you did befoe, and grinding and
polishing the cross sections carefully from coarse to fine.

When you observe the cross sections, it is easy to see what is the M-bond
and what is the other thing you want to see.

Just a suggestion.

Good luck!

Zhenquan Liu


=================

Does anyone have suggestions as to how to polish silicon wafers in cross
section. I have a wafer (~0.5mm thick ) which has been randomly fractured
with about a micron of metal on the surface and want to determine the metal
thickness. We are trying various methods - first choice is by RBS but
presently limited in max. energy so can not penetrate layer.

In my first couple of attempts at polishing (embedded wafer in thermoset)
the silicon breaks quite easily producing a very rough surface. I assume I
am being too aggressive and am trying slower more gentle procedure. Does
anyone have a procedure for polishing cross sections of such brittle material?

Thanks,
Ed

------------------------------------
Zhenquan Liu (Dr.)
Arizona State University
Room 146A, CSSS
Tempe, AZ 85287
Tel (480) 965 4544 (o)
(480) 775 7428 (h)
Fax (480) 965 9004 (o)
Email zhenquan.liu-at-asu.edu
------------------------------------




From daemon Wed Dec 6 14:05:17 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 06 Dec 2000 16:34:41 +0000
Subject: Re: SEM - Choice of buffer

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Bart

I have used PIPES as a substitute for cacodylate in most routine procedures
simply because it has none of the precipitation problems of phosphate
buffers. I understand that there may be more extraction with cacodylate in
TEM so I would have thought that it may be a better buffer for SEM (less
chance of shrinkage?). I would assume that HEPES was similar but have no
experience with it.

These are my own feelings and I have seen no scientific evidence to support
this - has anyone else?


Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Bart De Pauw wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Hello listers,
}
} We use cacodylate buffer for our SEM samples. Is HEPES or PIPES buffer
} an alternative for the toxic cacodylate ? Our samples are mainly animal
} tissue.
}
} Bart De Pauw
} Ghent University
} Faculty of Veterinary Medicine
} Department Morphology
} Belgium



From daemon Wed Dec 6 17:20:39 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 06 Dec 2000 15:16:20 -0800
Subject: Fwd: Re: MORE SERIAL SECTIONS

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} To: Timothy.Schneider-at-Mail.TJU.EDU
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: MORE SERIAL SECTIONS
}
} If you are not limited by particular area in your sample, you may do the
} trick, which warranty the parallelness of the edges. This trick did show
} to me Prof. Borovyagim many years ago. The trick is: you do not form a
} trapezoid, instead you make two cuts by razor blade, which owerlapping, so
} you will have "/\"-shape without flat top. The top should be formed by
} two overlapped cuts. There is no flat space on the top, which we usually
} called "trapezoid". This two cuts will form a "line" which will be
} paralle to the knife edge later. You have to form side fases of the
} piramid as well. Finall, you will have something looks pretty like
} triangle prizm. Not perfect prizm, of coarse. When you start cutting,
} you have to carefully cut avay the top of the "prizm" to form
} trapezoid. The final shape of the trapezoid will depends from orientation
} of your cuts, but owerlapped cuts will form perfectly parallel sides.
}
} Another choice I friequently use: to use glass knife and trim on the
} goniometer-head when you may tilt and rotate the sample. Make one face,
} rotate 180 deg and make second face.
}
} At 09:24 AM 12/6/00 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Dec 6 17:20:40 2000



From: Phillip Rutledge :      prutledge-at-msa-stoneville.ars.usda.gov
Date: Thu, 06 Apr 2000 14:37:21 -0500
Subject: LM--TISSUE PROCESSORS

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I am currently in the process of setting up a histology/microbiology/EM facility. Does anyone have comments or suggestions on a good rotary tissue processor for light microscopy? My main concerns are cost, service availability, ease in operation(changing of fluids, etc.) and reliability. I may process 10 - 60 specimens a month, more as the lab is established. I appreciate any info.

Thanks,

Phil Rutledge
prutledge-at-ars.usda.gov


From daemon Wed Dec 6 17:20:40 2000



From: Phillip Rutledge :      prutledge-at-msa-stoneville.ars.usda.gov
Date: Thu, 13 Apr 2000 06:38:28 -0500
Subject: TISSUE PROCESSORS

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I'm currently in the process of setting up a Histology/Microbiology/EM Lab. Having been away from histology for almost 30 years (been doing EM), I've gotten away from instrumentation associated with histology. Does anyone have a good recommendation for a rotary tissue processor? I've got info on Shandon and Tissue-Tek. Are there others out there?
Thanks for any info.

Phil
PRUTLEDGE-at-ARS.USDA.GOV




From daemon Wed Dec 6 17:22:05 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 06 Dec 2000 15:20:52 -0800
Subject: Fwd: Re: MORE SERIAL SECTIONS

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} Date: Wed, 06 Dec 2000 15:16:31 -0800
} To: Timothy.Schneider-at-Mail.TJU.EDU
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: MORE SERIAL SECTIONS
}
} If you are not limited by particular area in your sample, you may do the
} trick, which warranty the parallelness of the edges. This trick did show
} to me Prof. Borovyagin many years ago. The trick is: you do not form a
} trapezoid, instead you make two cuts by razor blade, which overlapping, so
} you will have "/\"-shape without flat top. The top should be formed by
} two overlapped cuts without "free" space between. There is no flat space
} on the top, which we usually called "trapezoid". This two cuts will form
} a "line" which will be parallel to the knife edge later. You have to
} form side faces of the pyramid as well. Finally, you will have something
} looks pretty like triangle prism. Not perfect prism, of coarse. When you
} start cutting, you have to carefully cut away the top of the "prism" to
} form trapezoid. The final shape of the trapezoid will depends from
} orientation of your cuts, but overlapped cuts will form perfectly parallel
} sides. This technique works if sample will permit.
}
} Another choice I frequently use: to use glass knife and trim on the
} goniometer-head when you may tilt and rotate the sample. Make one face,
} rotate 180 deg and make second face. If I have enough time, I prefer this
} way, almost gives me the perfect pyramid.
}
} Sergey
}
} At 09:24 AM 12/6/00 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Dec 6 17:22:48 2000



From: Phillip Rutledge :      prutledge-at-msa-stoneville.ars.usda.gov
Date: Mon, 17 Apr 2000 10:27:05 -0500
Subject: LM - Tissue Processors

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I'm currently in the process of setting up a Histology/Microbiology/EM facility. Being out of Histology for almost 30 years (been doing EM) I'm not sure of the best type of tissue processor to buy. I won't be doing that many specimens, so I'm leaning towards a rotary tissue processor. I have info on Shandon and Tissue-Tek. Are there others out there? Anyone having opinions on the 2 mentioned, I would appreciate any comments. I am looking for ease of use (changing solutions, fume safeguards), reliability on service and cost effectiveness. Thanks.

Peace be with you,
Phil Rutledge (410)778-4136, 2120
prutledge-at-ars.usda.gov


From daemon Wed Dec 6 17:25:25 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 6 Dec 2000 17:21:51 -0600
Subject: RE: Wafer Cross sections

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I usually glue (with superglue or thin epoxy) 2-4 wafers together, metal coated
surfaces facing each other. Then embed them or mount in microwise, cut with low
speed diamond saw and polish, preferably with diamond. If you do not need nice
pictures, just measurements, do not use fine polish, you will more or less ruin
edges with it.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Ed Kurz [mailto:ekurz-at-mail.ims.uconn.edu]
} Sent: Tuesday, December 05, 2000 8:32 AM
} To: MICROSCOPY BB
} Subject: Wafer Cross sections
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Does anyone have suggestions as to how to polish silicon
} wafers in cross
} section. I have a wafer (~0.5mm thick ) which has been
} randomly fractured
} with about a micron of metal on the surface and want to
} determine the metal
} thickness. We are trying various methods - first choice is by RBS but
} presently limited in max. energy so can not penetrate layer.
}
} In my first couple of attempts at polishing (embedded wafer
} in thermoset)
} the silicon breaks quite easily producing a very rough
} surface. I assume I
} am being too aggressive and am trying slower more gentle
} procedure. Does
} anyone have a procedure for polishing cross sections of such
} brittle material?
}
} Thanks,
} Ed
}


From daemon Thu Dec 7 00:18:20 2000



From: J. A. Kiernan :      jkiernan-at-julian.uwo.ca
Date: Thu, 7 Dec 2000 01:03:27 -0500 (EST)
Subject: Re: Gurr buffer recipe?

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On Wed, 6 Dec 2000, Tamara Howard wrote:

} Would anyone happen to have the actual recipe for Gurr's buffer? I think
} it is some phosphate formulation ...
} I know we can buy the pre-made buffer, but we need such a tiny amount that
} we'd rather make it from scratch (plus we want to know what it is!).

Quite right too! (For making and wanting to know)
I haven't heard of it so can't tell you the answer, BUT:

Does it matter what the exact formulation is? It is unlikely
that either of the late Gurr Bros (Edward, and George T.) invented
an original buffer. They were vendors of stains in Britain, about
whom some older HistoNetters may well have entertaining anecdotes.

If a buffer does its job of stabilizing the pH at the correct
value, and doesn't contain anything that would react with the
other ingredients of the mixture, it shouldn't matter what you
use. Phosphate buffers make a precipitate with many metal salts,
including those of Ca, Co, Cu, Fe, Mg, and others that have
insoluble phosphates. All the techniques books published since
about 1955 have sets of tables for making buffers to cover a
wide range of pH.

John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1




From daemon Thu Dec 7 00:22:26 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 06 Dec 2000 22:18:27 -0800
Subject: Re: Negative stain

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Hello Debby

Ted Pella Poly-L-Lysin 0.1% w/v aqueous solution, Cat # 18026
Float your grid with film on small drop of the solution for 1 min and than
remove excess of the liquid by filter paper and put on the drop of water
for 5-10 sec, blot it and immediately move it on the drop with your sample.
Temperature - as necessary for your sample. It also helps to adsorb tuff
samples. Surprisingly, it seems to me that it does not affect background.

Concerning "double-carbon" technique. It is more tricky. The idea is to
sandwich your sample between two carbon films just before you finish the
staining. I prefer the following technique. The approx. 1 ml plastic cap
(have no idea what is that, you may use any suitable container) 0.7 cm
diameter I filled with 0.5-1% aqueous UA in the way to make flat meniscus,
so it will reflect the light. Than I will cut 3x3 mm piece of mica with
carbon film and float carbon on the UA solution. Reflecting light will
makes it visible under some particular angle (you have to practice a little
bit). So, you have the plastic cap with UA and floated piece of carbon film
on it and you can see carbon easily. Than you will adsorb sample on the
regular EM grid with carbon. Than you have to move your grid into the cap
with stain (avoid contact to the floated carbon, soak grid into the cap
away from carbon). Looking through the carbon film, still floated on the
UA, move the grid just under the film. So, you will see the grid through
floated film. Than using one smooth movement you have to move grid
vertically up picking up the carbon film. So, you will put grid just under
the film (deep under the surface) and than move grid vertically up. The
film will holds over the grid with drop of the stain solution. The last
most important step - to remove excesses of staining solution: fix grid
securely in the tweezer by rubber O-ring (or use "reverse-action" tweezer),
small triangle shape piece of filter paper (I am using Whatmann 1M paper)
insert between tweezer's "legs" (sorry, guys, I have no idea how it is
called) as closer to the grid as possible. Filter paper chip should
contact with excess of liquid holds by capillary force between the
tweezer's "legs". Keep tweezer with grid and filter paper until grid will
dry completely (at least 5 min). Enjoy the perfect spreading of the
stain! There is a number of disadvantages in this method:
-"double-carbon" will decrease the resolution;
-sandwich will slightly flat the particles, expect to see 10-15% bigger
size because of that.

Advantages:
-sandwich will protect your sample from radiation damage;
-such samples usually are more stable from all points of view;
-because of thick carbon, you may use 400 mesh naked EM grids without
"holey" support film (I still prefer to use the grids with my home-made
"holey" film).

PTA vs AM. In my point of view, PTA does not have any serious advantages
over the AM. Personally, I prefer AM. In some particular cases PTA may
work better, I guess. If there is no chance to use UA, I would try
U-formiate first and oxalate - next. U-formiate (UF) has pH around 6 or
even higher (I don't remember the exact number). It gives you a very nice
fine staining (I love it better than UA). The disadvantage of this stain -
it is not stable. You have to prepare fresh solution every time. It is
light-sensitive also (general room illumination is OK for 20-30 min, no
direct light, please!). You could store it in the dark in the ice for
couple of hours. U-oxalate (UO) is less stable than UF, you have to use it
immediately. The beauty of UO - you may use it at pH 7. People's
unsuccess with UF or UO mostly related to the storage problem, I
believe. Most people don't understand how unstable those substances
are. By the way, UA is light-sensitive also.

My staining conditions for all stains are: +4oC (if sample permits),
adsorption time - 1-2 min; staining 0.5-1% aqueous solution - 1-2 min. I
find, that it is better to adjust sample concentration rather than to play
with adsorption time. Extended staining time will not dramatically improve
the staining but may harm the sample's integrity (it depends, for IgG
staining I am using 4 min 1% UA). For UA staining I also prefer to use my
favorite "EM" buffer based on ammonium acetate with additives if necessary
(50-100 mM ammonium acetate, pH 7.8 in simplest case). It is highly
compatible with UA and gives good stain's distribution.

I am sorry if my description is not clear. Please, feel free to call me if
any question. It's much simpler to do than read.

God luck
Sergey



At 11:08 AM 12/6/00 -0500, you wrote:
} Sergey,
} Would you mind giving me the details of your treatment of grids with
} poly-lysine and the double-carbon technique that you refer to in your
} E-mail. I usually go to PTA if PH is a problem. How do you compare PTA
} with AM? I am always looking for new methods to deal with some of the
} negative stain. problems.
} Thanks very much,
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail:
} dsherman-at-purdue.edu
} 1057 Whistler Building
} West Lafayette, IN 47907-1057
}
}
} On Tuesday, December 5, 2000 10:43 PM, Sergey Ryazantsev
} {sryazant-at-ucla.edu} wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Ammonium molybdate (AM) is suitable in the cases when uranyl acetate (UA)
} } is bad (mostly if pH is a critical factor, UA pH = 5-5.5). In most cases AM
} } gives less contrast than UA. AM gives you "pure" negative staining because
} } do not chemically interfere with most biological samples. UA from another
} } hand sometimes gives you mixed contrast, positive
} } (DNA/RNA)&negative(proteins). For instance, ribosomes and many viruses
} } shown mixed staining with UA. It's not bad because it may even enhance
} } some structural details, but you have to remember about that. My own
} } experience indicated that in most cases UA or other uranium salts
} } (U-formiate or oxalate) after conditions adjusting (concentration, time,
} } temperature, freshness, support film) has shown perfect results. If you
} } have a problem with stain spreading, you may try different support films
} } (carbon, formvar, parlodion). Very good results you may obtain using
} } "double carbon" technique - this may solv even very worse cases. I also
} } has have a good results with poly-lysine treatment of the grids prior
} } sample adsorption. Personally, I don't like the glow-discharge. But it
} } works in some way.
} }
} } Sergey.
} }
} } At 11:52 AM 12/5/00 -0800, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear all ,
} } } Does any body use Ammonium Molybdate for Negative staining and also by
} } } this chemical visualize structure better then Uranyl acetate.
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Dec 7 01:25:18 2000



From: Bart De Pauw :      Bart.DePauw-at-rug.ac.be
Date: Thu, 07 Dec 2000 08:17:56 +0100
Subject: Re: LM - Tissue Processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use the Shandon Citadel 1000. It's a good processor, but there's one disadvantage : there's evaporation of your fluids. There's no fume safeguard on the processor, so you have to put it in a fume hood.

Phillip Rutledge schreef:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm currently in the process of setting up a Histology/Microbiology/EM facility. Being out of Histology for almost 30 years (been doing EM) I'm not sure of the best type of tissue processor to buy. I won't be doing that many specimens, so I'm leaning towards a rotary tissue processor. I have info on Shandon and Tissue-Tek. Are there others out there? Anyone having opinions on the 2 mentioned, I would appreciate any comments. I am looking for ease of use (changing solutions, fume safeguards), reliability on service and cost effectiveness. Thanks.
}
} Peace be with you,
} Phil Rutledge (410)778-4136, 2120
} prutledge-at-ars.usda.gov



From daemon Thu Dec 7 02:37:27 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 07 Dec 2000 00:32:20 -0800
Subject: Re: Negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris, thanks for your compliments.

I did not publish this technique yet. So, you may refer to our ListServer.
If you need more details - call me.

Sergey.

At 07:52 AM 12/7/00 +0000, you wrote:
} Brilliant, Sergey. Have you published these methods anywhere? If
} so, I would be interested to have the references.
} Best wishes
} Chris
}
} Date sent: Wed, 06 Dec 2000 22:18:27 -0800
} To: Debby Sherman {dsherman-at-purdue.edu}
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: Negative stain
} Copies to: Microscopy-at-sparc5.microscopy.com
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello Debby
} }
} } Ted Pella Poly-L-Lysin 0.1% w/v aqueous solution, Cat # 18026
} } Float your grid with film on small drop of the solution for 1 min and than
} } remove excess of the liquid by filter paper and put on the drop of water
} } for 5-10 sec, blot it and immediately move it on the drop with your
} sample.
} } Temperature - as necessary for your sample. It also helps to adsorb tuff
} } samples. Surprisingly, it seems to me that it does not affect background.
} }
} } Concerning "double-carbon" technique. It is more tricky. The idea is to
} } sandwich your sample between two carbon films just before you finish the
} } staining. I prefer the following technique. The approx. 1 ml plastic cap
} } (have no idea what is that, you may use any suitable container) 0.7 cm
} } diameter I filled with 0.5-1% aqueous UA in the way to make flat meniscus,
} } so it will reflect the light. Than I will cut 3x3 mm piece of mica with
} } carbon film and float carbon on the UA solution. Reflecting light will
} } makes it visible under some particular angle (you have to practice a
} little
} } bit). So, you have the plastic cap with UA and floated piece of carbon
} film
} } on it and you can see carbon easily. Than you will adsorb sample on the
} } regular EM grid with carbon. Than you have to move your grid into the cap
} } with stain (avoid contact to the floated carbon, soak grid into the cap
} } away from carbon). Looking through the carbon film, still floated on the
} } UA, move the grid just under the film. So, you will see the grid through
} } floated film. Than using one smooth movement you have to move grid
} } vertically up picking up the carbon film. So, you will put grid just
} under
} } the film (deep under the surface) and than move grid vertically up. The
} } film will holds over the grid with drop of the stain solution. The last
} } most important step - to remove excesses of staining solution: fix grid
} } securely in the tweezer by rubber O-ring (or use "reverse-action"
} tweezer),
} } small triangle shape piece of filter paper (I am using Whatmann 1M paper)
} } insert between tweezer's "legs" (sorry, guys, I have no idea how it is
} } called) as closer to the grid as possible. Filter paper chip should
} } contact with excess of liquid holds by capillary force between the
} } tweezer's "legs". Keep tweezer with grid and filter paper until grid will
} } dry completely (at least 5 min). Enjoy the perfect spreading of the
} } stain! There is a number of disadvantages in this method:
} } -"double-carbon" will decrease the resolution;
} } -sandwich will slightly flat the particles, expect to see 10-15% bigger
} } size because of that.
} }
} } Advantages:
} } -sandwich will protect your sample from radiation damage;
} } -such samples usually are more stable from all points of view;
} } -because of thick carbon, you may use 400 mesh naked EM grids without
} } "holey" support film (I still prefer to use the grids with my home-made
} } "holey" film).
} }
} } PTA vs AM. In my point of view, PTA does not have any serious advantages
} } over the AM. Personally, I prefer AM. In some particular cases PTA may
} } work better, I guess. If there is no chance to use UA, I would try
} } U-formiate first and oxalate - next. U-formiate (UF) has pH around 6 or
} } even higher (I don't remember the exact number). It gives you a very nice
} } fine staining (I love it better than UA). The disadvantage of this
} stain -
} } it is not stable. You have to prepare fresh solution every time. It is
} } light-sensitive also (general room illumination is OK for 20-30 min, no
} } direct light, please!). You could store it in the dark in the ice for
} } couple of hours. U-oxalate (UO) is less stable than UF, you have to
} use it
} } immediately. The beauty of UO - you may use it at pH 7. People's
} } unsuccess with UF or UO mostly related to the storage problem, I
} } believe. Most people don't understand how unstable those substances
} } are. By the way, UA is light-sensitive also.
} }
} } My staining conditions for all stains are: +4oC (if sample permits),
} } adsorption time - 1-2 min; staining 0.5-1% aqueous solution - 1-2 min. I
} } find, that it is better to adjust sample concentration rather than to play
} } with adsorption time. Extended staining time will not dramatically improve
} } the staining but may harm the sample's integrity (it depends, for IgG
} } staining I am using 4 min 1% UA). For UA staining I also prefer to use my
} } favorite "EM" buffer based on ammonium acetate with additives if necessary
} } (50-100 mM ammonium acetate, pH 7.8 in simplest case). It is highly
} } compatible with UA and gives good stain's distribution.
} }
} } I am sorry if my description is not clear. Please, feel free to call
} me if
} } any question. It's much simpler to do than read.
} }
} } God luck
} } Sergey
} }
} }
} }
} } At 11:08 AM 12/6/00 -0500, you wrote:
} } } Sergey,
} } } Would you mind giving me the details of your treatment of grids with
} } } poly-lysine and the double-carbon technique that you refer to in your
} } } E-mail. I usually go to PTA if PH is a problem. How do you compare PTA
} } } with AM? I am always looking for new methods to deal with some of the
} } } negative stain. problems.
} } } Thanks very much,
} } } Debby
} } }
} } } Debby Sherman Phone: 765-494-6666
} } } Life Science Microscopy Facility FAX: 765-494-5896
} } } Purdue University E-mail:
} } } dsherman-at-purdue.edu
} } } 1057 Whistler Building
} } } West Lafayette, IN 47907-1057
} } }
} } }
} } } On Tuesday, December 5, 2000 10:43 PM, Sergey Ryazantsev
} } } {sryazant-at-ucla.edu} wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Ammonium molybdate (AM) is suitable in the cases when uranyl acetate
} (UA)
} } } } is bad (mostly if pH is a critical factor, UA pH = 5-5.5). In most
} cases AM
} } } } gives less contrast than UA. AM gives you "pure" negative staining
} because
} } } } do not chemically interfere with most biological samples. UA from
} another
} } } } hand sometimes gives you mixed contrast, positive
} } } } (DNA/RNA)&negative(proteins). For instance, ribosomes and many viruses
} } } } shown mixed staining with UA. It's not bad because it may even enhance
} } } } some structural details, but you have to remember about that. My own
} } } } experience indicated that in most cases UA or other uranium salts
} } } } (U-formiate or oxalate) after conditions adjusting (concentration,
} time,
} } } } temperature, freshness, support film) has shown perfect results. If you
} } } } have a problem with stain spreading, you may try different support films
} } } } (carbon, formvar, parlodion). Very good results you may obtain using
} } } } "double carbon" technique - this may solv even very worse cases. I also
} } } } has have a good results with poly-lysine treatment of the grids prior
} } } } sample adsorption. Personally, I don't like the glow-discharge. But it
} } } } works in some way.
} } } }
} } } } Sergey.
} } } }
} } } } At 11:52 AM 12/5/00 -0800, you wrote:
} } } } } --------------------------------------------------------------------
} ----
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } --------------------------------------------------------------------
} ---.
} } } } }
} } } } }
} } } } } Dear all ,
} } } } } Does any body use Ammonium Molybdate for Negative staining and also by
} } } } } this chemical visualize structure better then Uranyl acetate.
} } } }
} } } } _____________________________________
} } } }
} } } } Sergey Ryazantsev Ph. D.
} } } } Electron Microscopy
} } } } UCLA School of Medicine
} } } } Department of Biological Chemistry
} } } } Box 951737
} } } } Los Angeles, CA 90095-1737
} } } }
} } } } Phone: (310) 825-1144
} } } } Pager: (310) 845-0248
} } } } FAX (departmental): (310) 206-5272
} } } } mailto:sryazant-at-ucla.edu
} } } }
} } } }
} } } }
} } } }
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr Chris Jeffree
} University of Edinburgh
} Biological Sciences EM Facility
} Daniel Rutherford Building
} King's Buildings EDINBURGH EH9 3JH
} Tel: +44 (0) 131 650 5345
} FAX: +44 (0) 131 650 6563
}
} Inveresk Cottage, 26 Carberry Road,
} Inveresk, Musselburgh, Midlothian EH21 8PR, UK
} Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
} FAX: +44 (0) 131 653 6248
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu Dec 7 03:02:50 2000



From: Sylke Helbing :      Sylke.Helbing-at-embl-heidelberg.de
Date: Thu, 7 Dec 2000 09:56:18 +0100
Subject: EM - Course on Electron Microscopy, Immunocytochemistry and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EMBO Practical Course on Electron Microscopy, Immunocytochemistry and
Stereology for Cell Biology at EMBL, Heidelberg, Germany, Aug 30 - Sep 8,
2001

This is a course about the production of thin sections of biological
material and their use in studying ultrastructure in the context of
molecular cell biology. The course will introduce the techniques of
cryosectioning, rapid freezing methods as an alternative to chemical
fixation, freeze substitution and resin embedding. It will instruct
participants in the sectioning of suitably prepared material and will then
concentrate on the use of these sections for localizing specific molecules
within cells. Ample time will be given to hands on practical work as well
as formal and informal discussion of available techniques for the
localization of subcellular molecules.

Further info: http://www.EMBL-Heidelberg.DE/courses/ElectronMicroscopy01/

****************************
Sylke Helbing
Course and Conference Office
European Molecular Biology Laboratory
Meyerhofstr. 1
D-69117 Heidelberg

Tel: +49-6221-387 106
Fax: +49-6221-387 158
Email: helbing-at-embl-heidelberg.de
****************************




From daemon Thu Dec 7 04:41:47 2000



From: HARRISm-at-esm-semi.co.uk
Date: Thu, 07 Dec 2000 10:31 +0000 (GMT)
Subject: Coating v's Bonding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi ,

RE: Semiconductor back end processes

A general question whether anyone knows where I can obtain information
on the metallisation or backside coating (through evaporation) of
silicon wafers after backgrinding ?
My limited previous metals coating experience has given me the
impression that true metal bonding is the result of the formation of a
intermetallic/segregated region - of both metallic constituents
cooling to form what is basically a weld zone with no discernible
porosity interface .
A mechanical bond on the other hand would simply be the 'knitting' of
rough surfaces .
My interest includes the goal of successful metallisation and how it
is assessed as normal mechanical preparation of a cross section can
tear and smear the face causing difficulty in porosity / interface
evaluation . Is FIB used or are there non destructive methods for
example ?


Thanks

Martyn Harris
ESM LTD
Cardiff Rd, Newport , Gwent
South Wales , UK .
Email : harrism-at-esm-semi.co.uk



From daemon Thu Dec 7 07:20:48 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 7 Dec 2000 05:14:48 -0800 (PST)
Subject: Re: SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Malcolm:

I don't think the chloroform has gotten more toxic (unless I forgot about
that fact, having used that technique for more years than I can
remember....). Hopefully, a lot of us have gotten a tiny bit smarter about
exposure to potentially harmful chemicals. Actually, over the years I have
developed sensitivity to a number of chemicals in the EM Lab, and still tend
to be a little careless about exposure to vapors - especially formalin,
glut, osmium, xylene, acetone, EtOH. But I, too, have used the heat pens
for well over 20 years (ever since the first cautery pens became available
thru one of the EM suppliers), and urge individuals who do a lot of
sectioning to switch to the heat pens.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals

On Wed, 06 Dec 2000 17:20:07 +0000, Malcolm Haswell wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Timothy
}
} I would be a bit concerned about your use of chloroform to flatten
sections,
} especially in a small microtome room. We have used heat pens for the last
decade
} or so, because apparently the chloroform we use these days is a lot more
toxic
} than it used to be (as well as formaldehyde, glutaraldehyde, carbon
} tetrachloride etc).
}
} Malcolm Haswell
} e.m. unit
} School of Sciences
} University of Sunderland
} UK
}
} Timothy Schneider wrote:
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} } The following comments are for the person who is trying to cut serial
} } sections with Spurrs. I routinely cut serial sections with Spurrs
without
} } resorting to any kind of glue. Probably the most important thing is
how the
} } block is faced. I cut the trapezoid with a really long (.6 to .9 mm
base)
} } and the top of the trapezoid is almost the same length. What's really
} } critical here is that those two cuts are parallel.
} } The two sides of the trapezoid are very short (.1mm). I cut the
sections
} } with a Diatome knife and on a Reichert Ultracut E with a cutting speed
of
} } 0.8mm per second and a thickness setting of 80nm. I am doing this in
a
} } small room with a blasting air vent in the ceiling. I have cut up a
} } cardboard box and stuck it into the ceiling tiles in such a way that
the air
} } is diverted away from the microtome. I can pick up 15 to 25 sections in
a
} } row on a 0.4 X 2mm copper slotted grid coated with just formvar or
} } formvar/carbon. I have never been any good at making the support films
my
} } self, so I buy the slotted grids precoated from Ted Pella (catalog #s
01706,
} } 01806, or 01816). Before picking up the ribbon, I dip a stick into
} } chloroform and wave that over the sections, and they expand (or relax,
} } depending on your point of view). I have a bunch of self locking
tweezers
} } and after picking up the sections, I leave the grid with the sections
on it
} } locked in the tweezers until they dry (if you put a wet slotted grid
down on
} } filter paper it might break the film). Good luck, Tim
} }
} } Timothy G. Schneider
} } Director of Electron Microscopy
} } Department of Pathology
} } Room 229 Jefferson Hall
} } Thomas Jefferson University
} } 1020 Locust St.
} } Philadelphia Pa. 19107
} } 215-503-4798 work
} } 610-613-8170 cellular
} } timothy.schneider-at-mail.tju.edu
}
}





_______________________________________________________
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From daemon Thu Dec 7 07:31:58 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 7 Dec 2000 05:27:45 -0800 (PST)
Subject: Objectives for AO microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

I have an old American Optical Spencer microscope (rescued from a trash
bin). It is a binoc, three objective turret (with 4x, 10x, 20x) and
built-in light source scope. I have loaned the scope to a young man who is
being home schooled, and he has become really interested in microscopy, and
aquatic biology. He is at the point that he needs at least a 40x objective,
and all I have are the 3 that came with the scope. The only numbers I can
find on the scope are 1063-1AA and 1235, and neither is specifically
designated as the model or serial numbers. Can anyone direct me to a source
for objectives for this type of microscope? These old scopes used a
standard thread and tube length, so maybe there are non-manufacturer
objectives that I can put on the scope. I haven't been able to look thru an
Edmund Scientific catalog (there's one around here somewhere), but I would
hope that either a collector or someone at Leica could provide some
additional info.

Thanks in advance.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals





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From daemon Thu Dec 7 09:06:18 2000



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Tue, 5 Dec 2000 19:51:05 -0600
Subject: Fw: Wavefront Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi James,

In principle, wave front reconstruction can be done by:

1) Off-axis holography: This methods need a special biprism for
overlapping of two beams (one passed the specimen, the other one passed
a hole). For the wavefront construction are two commercial programs
available - one from Gatan and the other one from our company. The group
around Prof. Hannes Lichte in Dresden is very active in this field - you
should contact him, if you want to know details.

2) Focal variation: This method reconstructs the wave front from a focal
series with very small defocus steps. For the reconstruction is at least
one commercial program available - you should contact Andreas Thust in
Juelich, Germany, if you want to know more about it. Also the group from
Prof. Dirk van Dyck in Antwerp is very active in this field.
The automatic acquisition of focal series is integrated in our programs
and we are supporting also the JEOL-4000 remote control, but we are
supporting only our cameras, no Gatan cameras. Sorry.

I apologize if I might have forgotten some references - I remember also
a poster from Michael O'Keefe in Philadelphia from this year, which was
related to this matter.

Regards,
Ingo

++++++++++++++++++++++++++++++++++++++++++++++++
Dr. Ingo Daberkow
Tietz Video and Image Processing Systems GmbH
D-82131 Gauting, Germany
Tel: +49-89-8506567
FAX: +49-89-8509488
Internet: www.tvips.com
Email: ingo.daberkow-at-tvips.com



-------- Original Message --------


forwarded from the imaging news group

"james patrick birrell" {jbirrell-at-students.uiuc.edu} wrote in message
news: {Pine.GSO.4.10.10012041635310.22505-100000-at-ux12.cso.uiuc.edu} ...
} I was wondering if anybody would know where I could obtain software to
} facilitate the acquisition/analysis of images to be used to perform
} wavefront reconstruction using images from a TEM. Specifically, I'm
} looking for any software compatable with a JEOL 4000ex microscope with a
} Gatan CCD camera.
} Thanks,
} James Birrell


From daemon Thu Dec 7 12:37:25 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Thu, 7 Dec 2000 10:32:30 -0800 (PST)
Subject: Re: SEM - Choice of buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have always understood that we use cacodylate buffer because it
penetrates tissue faster and further than other buffers, taking the
fixative with it. Plus, of course, the advantages that it does not form a
precipitate with osmium, and that if you need to store tissues after
fixing, then it discourages the growth of microorganisms. It is toxic, but
surely not as much so as glutaraldehyde or OsO4, which we can't really
avoid in EM. So, I would suggest we all go on using it, and take all the
proper precautions for this and the other toxic agents that we need.

Lesley Weston.



On Wed, 6 Dec 2000, Malcolm Haswell wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Bart
}
} I have used PIPES as a substitute for cacodylate in most routine procedures
} simply because it has none of the precipitation problems of phosphate
} buffers. I understand that there may be more extraction with cacodylate in
} TEM so I would have thought that it may be a better buffer for SEM (less
} chance of shrinkage?). I would assume that HEPES was similar but have no
} experience with it.
}
} These are my own feelings and I have seen no scientific evidence to support
} this - has anyone else?
}
}
} Malcolm Haswell
} e.m. unit
} School of Sciences
} University of Sunderland
} UK
}
} Bart De Pauw wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hello listers,
} }
} } We use cacodylate buffer for our SEM samples. Is HEPES or PIPES buffer
} } an alternative for the toxic cacodylate ? Our samples are mainly animal
} } tissue.
} }
} } Bart De Pauw
} } Ghent University
} } Faculty of Veterinary Medicine
} } Department Morphology
} } Belgium
}
}
}



From daemon Thu Dec 7 12:37:26 2000



From: Todd Kostman :      kostman-at-vaxa.cis.uwosh.edu
Date: Thu, 07 Dec 2000 12:29:29 -0600
Subject: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear EMers,

I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
to the scopes in our facility. Both the previous director and I feel that
we do not get our monies worth, so to speak, from this dewar. I had our
local supplier look at it and they say it is fine. Once it gets to 1/2
tank,the remaining LN vaporizes and bleeds off within a day. The last time
I had it filled, I calculated that I used about 50L of the 160L before it
went dry. Once it got down to half, I dumped the remaining LN into a 34L
dewar (and it is stll there). Does everyone have pretty much the same
experience with these 160L dewars?

Thanks for your help, and Merry Christmas to all microscopists (who are the
salt of the earth!)


Sincerely,


Todd

Dr. Todd A. Kostman
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
University of Wisconsin Oshkosh
800 Algoma Boulevard
Oshkosh, WI 54901-8640
ph: (920) 424-3069
fax: (920) 424-1101
kostman-at-uwosh.edu




From daemon Thu Dec 7 12:53:18 2000



From: S. Kuehner :      kuehner-at-u.washington.edu
Date: Thu, 7 Dec 2000 10:50:53 -0800 (PST)
Subject: Re: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, I have also found 160L dewars NOT to be cost effective with only a
small user base. I switched to a 35L dewar many years ago. Even with the
more frequent deliveries I'm saving money over the 160L.

************************************************
### Free the Psoas ###
**************

Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************

On Thu, 7 Dec 2000, Todd Kostman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear EMers,
}
} I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
} to the scopes in our facility. Both the previous director and I feel that
} we do not get our monies worth, so to speak, from this dewar. I had our
} local supplier look at it and they say it is fine. Once it gets to 1/2
} tank,the remaining LN vaporizes and bleeds off within a day. The last time
} I had it filled, I calculated that I used about 50L of the 160L before it
} went dry. Once it got down to half, I dumped the remaining LN into a 34L
} dewar (and it is stll there). Does everyone have pretty much the same
} experience with these 160L dewars?
}
} Thanks for your help, and Merry Christmas to all microscopists (who are the
} salt of the earth!)
}
}
} Sincerely,
}
}
} Todd
}
} Dr. Todd A. Kostman
} Assistant Professor of Biology and Microbiology
} Director, Electron Microscopy Facility
} University of Wisconsin Oshkosh
} 800 Algoma Boulevard
} Oshkosh, WI 54901-8640
} ph: (920) 424-3069
} fax: (920) 424-1101
} kostman-at-uwosh.edu
}
}
}
}



From daemon Thu Dec 7 13:42:00 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Dec 2000 13:34:24 -0600
Subject: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We have had a similar experience here, going to a 160L dewar after having to
make a trip across campus with a 30L dewar everytime we needed LN2. The
huge discount on the 160L container means we're paying about the same per
liter actually used as we were before, plus we no longer have the
aggravation of having to go and get it.

One discovery we made, however, is that by transferring LN2 from the 160L
dewar to smaller, non-pressurized ones we are able to keep the nitrogen
much, much longer. We have 10L, 50L and 30L containers in our lab, so now
when a new 160L dewar is delivered, we fill up the smaller ones immediately,
then use the 160L until it's gone. The smaller dewars will keep LN2
literally for weeks with minimal losses. Initially we were averaging 60
usable liters per 160 delivered, but now we are probably up way past 100 (I
haven't actually figured it out.).

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu]
Sent: Thursday, December 07, 2000 12:29 PM
To: Microscopy Listserver


Dear EMers,

I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
to the scopes in our facility. Both the previous director and I feel that
we do not get our monies worth, so to speak, from this dewar. I had our
local supplier look at it and they say it is fine. Once it gets to 1/2
tank,the remaining LN vaporizes and bleeds off within a day. The last time
I had it filled, I calculated that I used about 50L of the 160L before it
went dry. Once it got down to half, I dumped the remaining LN into a 34L
dewar (and it is stll there). Does everyone have pretty much the same
experience with these 160L dewars?

Thanks for your help, and Merry Christmas to all microscopists (who are the
salt of the earth!)


Sincerely,


Todd

Dr. Todd A. Kostman
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
University of Wisconsin Oshkosh
800 Algoma Boulevard
Oshkosh, WI 54901-8640
ph: (920) 424-3069
fax: (920) 424-1101
kostman-at-uwosh.edu




From daemon Thu Dec 7 13:52:43 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 7 Dec 2000 13:33:39 -0600
Subject: Re: SEM - Choice of buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't agree. I seriously doubt that the diffusion rate of
cacodylate and the "Good buffers" (i.e., the buffers like PIPES,
HEPES, MES, that were designed by Dr. Good) were ever compared since
they are from different eras. I am also not sure I understand how
the diffusion rate of a buffer component "takes the fixative with it"
at the molecular level. Arsenic buffers do inhibit bacterial growth
but I have had bacteria grow in cacodylate buffers. Furthermore, if
you have aldehydes or osmium in your buffer, you have all the
anti-bacterial agent you need. Cacodylate buffers can give off toxic
gases when acidified but more importantly, you are adding needlessly
to the toxic waste stream. Aldehydes will eventually react or
breakdown into non-toxic substances but the arsenic will remain a
problem forever. Cacodylate was originally selected based on its pKa
and non-reactivity with fixatives. Phosphate caused problems with
calcium precipitation and Tris buffers had amino groups that react
with the fixatives. The choices for buffers were very limited in the
old days. Dr. Good made a significant contribution in his
description of this family of buffers. Anatomists tend to be the
most old fashioned scientists in the world.



}
}
} I have always understood that we use cacodylate buffer because it
} penetrates tissue faster and further than other buffers, taking the
} fixative with it. Plus, of course, the advantages that it does not form a
} precipitate with osmium, and that if you need to store tissues after
} fixing, then it discourages the growth of microorganisms. It is toxic, but
} surely not as much so as glutaraldehyde or OsO4, which we can't really
} avoid in EM. So, I would suggest we all go on using it, and take all the
} proper precautions for this and the other toxic agents that we need.
}
} Lesley Weston.
}
}
}
} On Wed, 6 Dec 2000, Malcolm Haswell wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Bart
} }
} } I have used PIPES as a substitute for cacodylate in most routine procedures
} } simply because it has none of the precipitation problems of phosphate
} } buffers. I understand that there may be more extraction with cacodylate in
} } TEM so I would have thought that it may be a better buffer for SEM (less
} } chance of shrinkage?). I would assume that HEPES was similar but have no
} } experience with it.
} }
} } These are my own feelings and I have seen no scientific evidence to support
} } this - has anyone else?
} }
} }
} } Malcolm Haswell
} } e.m. unit
} } School of Sciences
} } University of Sunderland
} } UK
} }
} } Bart De Pauw wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } Hello listers,
} } }
} } } We use cacodylate buffer for our SEM samples. Is HEPES or PIPES buffer
} } } an alternative for the toxic cacodylate ? Our samples are mainly animal
} } } tissue.
} } }
} } } Bart De Pauw
} } } Ghent University
} } } Faculty of Veterinary Medicine
} } } Department Morphology
} } } Belgium
} }
} }
} }

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu Dec 7 15:05:43 2000



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Thu, 07 Dec 2000 15:05:16 -0600
Subject: peripheral nerve voodoo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Friends,
Once a gain I need that voodoo that you do so well.

A colleague is asking how to embed, freeze and frozen-section (cross section) a rat sciatic perpheral nerve. Several attempts have failed to give adequate structural detail. Any tips and tricks are welcome.

Linda Fox
lfox1-at-lumc.edu


From daemon Thu Dec 7 15:05:55 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Thu, 7 Dec 2000 16:02:56 -0500
Subject: Re: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We got similar results when we monitored nitrogen use 10 years ago, and
again when we retested earlier this year. We tried two different 160 liter
tanks, one of which was brand new, and never got more than about 80 liters
out. I eventually gave up and went to four 50 liter tanks, which we
receive at atmospheric pressure and tap with a withdrawal device that self
pressurizes to about 9 psi. The device has a rubber (now teflon for better
thermal properties) transfer hose, so there is little loss due to warming
the hose. We usually get 40-45 liters out of these.

The losses, as I understand them, are from

1. passive losses from the tank itself (static evaporation rate) which are
higher for the 160 liter tanks
2. losses due to cooling of the transfer tube (lower in the 50 liter tank
with no phase separator)
3. depressurization loss, which is higher in the 160 liter tank pressurized
to 20 psi than for the 50 L tank at 9 psi.

Obviously if you need high pressure or use high volumes, the 160 liter tank
is better. Also, with the 50 liter tanks you have to plan ahead because it
takes some time to pressurize the tanks, but otherwise the 50 liter option
works for us.

Marie

the} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Thu Dec 7 16:00:32 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 7 Dec 2000 13:57:42 -0800
Subject: SEM: mystery CL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


... just for fun ... anyone know what this is?

(hint: image is electron induced cathodo-luminescence ... the
material is natural quartz ... the colors are false but
representative)

happy holidays, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Thu Dec 7 16:02:33 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 7 Dec 2000 14:00:21 -0800
Subject: SEM: mystery CL (oops)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


oops! ... forgot the URL ...*smile*...
http://epmalab.uoregon.edu/images/sem/mystery-CL.jpg


... just for fun ... anyone know what this is?

(hint: image is electron induced cathodo-luminescence ... the
material is natural quartz ... the colors are false but
representative)

happy holidays, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Fri Dec 8 03:37:50 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 08 Dec 2000 09:35:03 +0000
Subject: Re: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Todd

we seem to be the exception. I expect to recover about 100L from a 160L dewar
over a two to three week period. I'm sure we once got a near record 110L out
but occasionally fall a little below the 100. We still therefore find the 160L
storage dewar more convenient because the alternative would require more space,
higher delivery cost and the need for multiple trolleys for several 25 litre
dewars. We can normally get nitrogen almost to the bottom of the 160L dewar,
providing that we don't leave less than about 10L in it (I'm guessing).
The only down-side is that it is a pressure vessel and requires regular
maintenance and testing to meet UK regulations, but this means that the spring
loaded pressure relief valve is regularly checked (as well as the burst disk
valve of course). It does however mean that if our 160L dewar ever fails its
tests that we will probably just buy more 25L dewars instead of replacing it.

Good luck

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Todd Kostman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear EMers,
}
} I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
} to the scopes in our facility. Both the previous director and I feel that
} we do not get our monies worth, so to speak, from this dewar. I had our
} local supplier look at it and they say it is fine. Once it gets to 1/2
} tank,the remaining LN vaporizes and bleeds off within a day. The last time
} I had it filled, I calculated that I used about 50L of the 160L before it
} went dry. Once it got down to half, I dumped the remaining LN into a 34L
} dewar (and it is stll there). Does everyone have pretty much the same
} experience with these 160L dewars?
}
} Thanks for your help, and Merry Christmas to all microscopists (who are the
} salt of the earth!)
}
} Sincerely,
}
} Todd
}
} Dr. Todd A. Kostman
} Assistant Professor of Biology and Microbiology
} Director, Electron Microscopy Facility
} University of Wisconsin Oshkosh
} 800 Algoma Boulevard
} Oshkosh, WI 54901-8640
} ph: (920) 424-3069
} fax: (920) 424-1101
} kostman-at-uwosh.edu



From daemon Fri Dec 8 04:55:48 2000



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Fri, 8 Dec 2000 10:41:37 -0000
Subject: HP LJ IIIp - EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Many thanks to all who replied to this query. I should have thought of the
EDAX site, senile dementia is really kicking in now. Just as well I'm being
thrown on the scrap heap at Christmas.

ttfn, Chris


From daemon Fri Dec 8 07:11:50 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 8 Dec 2000 07:02:04 -0600
Subject: Re: Objectives for AO microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Roger,

I do not know if I can help, but I have a an excellent 50x oil AO
objective with a correction collar used on an AO MicroStar series compound
microscope. This is the scope with a grey finish. The numerical aperture
of this objective is 0.80 and with the correction collar exceeds the light
gathering capacity and resolution of any dry 40x or 63x dry objective.

Do you have any items you can perhaps trade, i.e., Zeiss objective lenses?

Let me know what you think.

Ken
-------------
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

Contact tel. number: 503-413-5391

On Thu, 7 Dec 2000, Roger Moretz wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings:
}
} I have an old American Optical Spencer microscope (rescued from a trash
} bin). It is a binoc, three objective turret (with 4x, 10x, 20x) and
} built-in light source scope. I have loaned the scope to a young man who is
} being home schooled, and he has become really interested in microscopy, and
} aquatic biology. He is at the point that he needs at least a 40x objective,
} and all I have are the 3 that came with the scope. The only numbers I can
} find on the scope are 1063-1AA and 1235, and neither is specifically
} designated as the model or serial numbers. Can anyone direct me to a source
} for objectives for this type of microscope? These old scopes used a
} standard thread and tube length, so maybe there are non-manufacturer
} objectives that I can put on the scope. I haven't been able to look thru an
} Edmund Scientific catalog (there's one around here somewhere), but I would
} hope that either a collector or someone at Leica could provide some
} additional info.
}
} Thanks in advance.
}
} Roger Moretz, Ph.D.
} Dept of Toxicology
} Boehringer Ingelheim Pharmaceuticals
}
}
}
}
}
} _______________________________________________________
} Tired of slow Internet? Get -at-Home Broadband Internet
} http://www.home.com/xinbox/signup.html
}
}
}




From daemon Fri Dec 8 07:46:41 2000



From: tellis2-at-hallmark.com
Date: Fri, 8 Dec 2000 07:41:45 -0600
Subject: Liq. nitrogen tanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I receive my liquid nitrogen in 160 L tanks from our supplier and they
generally last from 24 to 30 days with me filling up the EDX dewar twice a
week (about 3-4 liters). I have had some tanks that only lasted two weeks
and after I told our supplier about that they said the leak valve was
stuck open and they replaced or adjusted the leak valve as needed. You
should get your local supplier to inspect your tank and maybe replace or
adjust the leak valve. I would not adjust it myself unless you really know
what you are doing since an explosion is possible without proper pressure
release.
Terry Ellis
Hallmark Cards Inc.
e-mail: tellis2-at-hallmark.com



From daemon Fri Dec 8 07:57:03 2000



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Fri, 08 Dec 2000 08:57:59 -0500
Subject: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Happy holidays oh great wise ones of microscopy!

(Apologies to John Lennon)


Imagine all day sectioning,
it isn't hard to do.
Putting sections on bare 200 mesh grids
and heat fixing under a light to boot.

Imagine all the sections coming off
while you do the stain.
Oh oh
You have to figure out what's wrong,
while racking your brain.
If you all can help,
you'll help me from going insane.


Any suggestions will be greatly appreciated. Thanks is advance!

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Fri Dec 8 08:10:58 2000



From: Sandra Hancock :      skperkin-at-vt.edu
Date: Fri, 08 Dec 2000 10:26:13 -0500
Subject: endothelial cell junctions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi-

We have a post-doc that is interested in looking at tight junctions in
cultured endothelial cells. He has found a vague reference for using
silver stain to visualize endothelial cell junctions. Does anyone have any
experience with this technique? Can you provide some technical details or
point us to a reference? Thank you!!

Sandy Hancock
VMRCVM




From daemon Fri Dec 8 09:31:48 2000



From: Max Sidorov :      msv-at-nl.feico.com
Date: Fri, 8 Dec 2000 16:25:13 +0100
Subject: TEM - Updated: Contrast Transfer Function Freeware (ctfExplorer)

Contents Retrieved from Microscopy Listserver Archives
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Dear All:

ctfExplorer has been updated.
New features/improvements:
1. Now it is possible to export graphs to text (tab-delimited).
2. List-tree with the microscopes is wider now.
3. Tab stops in dialogs arranged in a logical sequence.
4. Now it calculates the Lichte-defocus. (Useful for holography).
5. Additional slider-control is added: allows to change "magnification",
i.e. convergence. Just to show people why it is not always a good idea to
work at 1,000,000x and why some people do high resolution work at as low as
50,000x magnification.

Here is the link: http://clik.to/ctfexplorer (always use this link. it will
direct you to the right location).

Enjoy,

Max Sidorov
---
(until December 31 2000)
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com (mailto:maxsidorov-at-bigfoot.com)

----------Additional Info----------
Dear All:
I wrote a piece of software which I believe would be of interest to the
microscopy community.
It's a highly interactive program which calculates/displays the contrast
transfer function of TEMs. I know that there are similar programs floating
around but ctfExplorer does not only 1d but also 2d calculations/display
with 2-fold and 3-fold astigmatism imposed. There are other unique features
to it. All parameters (defocus, voltage, Cs, etc) can be changed
interactively.

DISCLAIMER: I do work for FEI/Philips Electron Optics. However, this
software is not an official product of FEI and FEI is not responsible for
its distribution/support. This software is freeware.

To the best of my knowledge, the software does not have any nasty bugs. It
is tested under Windows 95/98 and Windows NT4.

Please give it a try. Please direct your suggestions and comments to
maxsidorov-at-bigfoot.com
I hope you'll find the software useful.

Here is the link: http://clik.to/ctfexplorer

Enjoy,

Max Sidorov
---
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com





From daemon Fri Dec 8 09:46:33 2000



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Fri, 8 Dec 2000 10:42:11 -0500
Subject: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
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Todd-
I have not calculated how much LN2 I am able to deliver from my 160 L
dewar, but it must be about 100 L since it will last me a month or more.
Perhaps my dewar is of a better grade as it is also certified for liquid He
use. I suspect that much of my LN2 loss is in cooling down the 10 L dewar
I use to transport it to the EDX dewar. Are you using a high pressure, or
low pressure dewar (mine has ~24psi max)? The high pressure dewars I have
had have an internal coil for warming up some liquid to maintain the head
pressure. If your dewar has this, there should be valves for closing off
this pressure generation coil which will extend the storage time. By the
way, what does your fill gauge read when the dewar is empty? These gauges
are frequently very inaccurate. The fact that you could fit the remainder
in a 34 L dewar when your gauge said 1/2 full, indicates to me that your
gauge probably isn't accurate. I hope that something in there helps...
Matt




Todd Kostman {kostman-at-vaxa.cis.uwosh.edu} on 12/07/2000 01:29:29 PM





To: Microscopy Listserver {Microscopy-at-sparc5.microscopy.com}
cc:


Dear EMers,

I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
to the scopes in our facility. Both the previous director and I feel that
we do not get our monies worth, so to speak, from this dewar. I had our
local supplier look at it and they say it is fine. Once it gets to 1/2
tank,the remaining LN vaporizes and bleeds off within a day. The last time
I had it filled, I calculated that I used about 50L of the 160L before it
went dry. Once it got down to half, I dumped the remaining LN into a 34L
dewar (and it is stll there). Does everyone have pretty much the same
experience with these 160L dewars?

Thanks for your help, and Merry Christmas to all microscopists (who are the
salt of the earth!)


Sincerely,


Todd

Dr. Todd A. Kostman
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
University of Wisconsin Oshkosh
800 Algoma Boulevard
Oshkosh, WI 54901-8640
ph: (920) 424-3069
fax: (920) 424-1101
kostman-at-uwosh.edu








From daemon Fri Dec 8 13:03:34 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Fri, 08 Dec 2000 13:57:38 -0500
Subject: coming unglued (sections, that is)

Contents Retrieved from Microscopy Listserver Archives
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Paula,
I loved your poem! I too , had the same problem with sections falling off
grids while staining, especially if using the thin bar grids. There's just
not enough surface area for the sections to stick to. I solved the
problem by putting the grids in the 60 degree oven overnight before
staining. I haven't lost any sections since, and I cut both very large and
small sections. The time in the oven doesn't seem to adversely affect the
tissue.

Good luck,
Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky


From daemon Fri Dec 8 13:03:55 2000



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Fri, 08 Dec 2000 14:05:12 -0500
Subject: Thanks to all & a little clarification

Contents Retrieved from Microscopy Listserver Archives
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Hi All,


Thanks to all who have already answered my plea. I think I need to make a few things clear. Me, in my effort to be witty forgot a few major details.


1. I'm cutting Spurr's embedded samples.

2. I'm cleaning the samples in a dilute acetic acid followed by an acetone chaser.

3. I've been heat fixing the grids/sections under a light for about 10 minutes.

4. I've been doing this for that past 10 months and things worked great until 3 weeks ago. I'm new to this area
(Washington, DC) having been in California doing EM for years. Could this be a climate/weather related
problem?

5. I will use formvar if I have to. Has anyone just dipped grids in formvar, instead of casting a film, to make the grids
a little sticky? I inherited a bunch of filthy formvar with water & junk in it and it takes about a month to get supplies.

6. I haven't changed my supplier of grids or Spurr's components. So I think that things should be OK on the quality
control end.


A lot of the suggestions I received are very good, but I have already tried most of them. Any bizarre rituals that I must do? I WILL NOT swing a dead cat over my head, but I might try other things. EM is basically voodoo, after all.

Thanks again,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Fri Dec 8 13:19:10 2000



From: DarrenScott Gray :      microscopy-at-Thunderdome.zzn.com
Date: Fri, 8 Dec 2000 14:13:59 -0500
Subject: please add me to the mailing list

Contents Retrieved from Microscopy Listserver Archives
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{html} {head} {meta Name='keywords' Content='commtouch, pronto, mail, free email, free, branded, web based, free web based email, communications, internet, software, advertising banners, e-mail, free software'} {/head} {body } {div align='left'} {font } {blockquote} {blockquote} {TT} Hello, {BR}
{BR}
Please add me to the microscopy discussion group. {BR}
{BR}
Thanks, {BR}
Darren {BR}
{BR}
{/TT} {br} {br} {font} {p align=left} {br} Get your Free E-mail at http://thunderdome.zzn.com {br} ____________________________________________________________ {br} Get your own FREE Web and POP E-mail Service in 14 languages at http://www.zzn.com. {br} {/blockquote} {/blockquote} {/div} {/font} {/body} {/html}


From daemon Fri Dec 8 13:37:14 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 8 Dec 2000 14:34:19 -0500
Subject: Re: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
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Hi Paula,
It is horrible to see naked grids come off the stain. We've probably
all been there. Here are my auggestions:

1. Make sure that your grids are clean to start. You can dip them in
acetone or ethanol (absolute) and then water and dry thoroughly.

2. Make sure that the sections have fully dried onto the grids. If
you're not rushed, just let them dry overnight. If you are rushed
(probably the usual situation), you can lay the grids out on a clean
glass slide that is resting on a warm (NOT HOT) hot plate (around
35-40deg.C) for a few minutes.

3. And as wierd as this seems....make sure that the water you are
using to make up your stains and to wash the grids is really clean
(deionized or double distilled or microfiltered). I had a friend to
used to come over to my building to fill a small carboy with water
because my dept. had better water treatment than hers. She swore
that her sections fell off when she used their water.

4. Maybe the simplest life saver....buy a "Coat-Quick G" grid coating
pen. It deposits a small amount of the "secret formula" adhesive
onto the grid bars, without blocking the spaces or causing problems
with dirt/stain deposition. I frequently cut enormous sections (by
EM standards) and this has been a blessing. I know that Electron
Microscopy Sciences sells them. Other companies probably do too.
Look in you favorite vendor's catalogue.


(Disclaimer: I have no financial interests in EMS)

Good luck,

Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Dec 8 13:43:11 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 8 Dec 2000 13:49:39 -0600 (CST)
Subject: Re: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
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Responding to the message of {sa30a2a7.040-at-gwise.gwumc.edu}
from "Paula Sicurello" {patpxs-at-gwumc.edu} :

Paula,


Imagine........ a magic solution........

GRID CLEANING SOLUTION

Clean your uncoated grids each time you set up to do sectioning, because newly
cleaned copper grids will oxidize within a day and sections will no longer stick
to them very well during staining and rinsing.


*ADD 40 ml CONCENTRATED HCL TO 400 ml DISTILLED WATER

*ADD 40 ml ACETONE TO THE ABOVE

*DILUTE TO 500 ml FINAL VOLUME

*SONICATE GRIDS IN THIS SOLUTION IN A 50 ml BEAKER FOR 1-2 MINUTES

*DISCARD USED SOLUTON DOWN DRAIN AND RINSE/SONICATE GRIDS FOR 30 SECONDS TWICE
WITH 100% ACETONE

*DUMP GRIDS ONTO CLEAN FILTER PAPER TO DRY

* ITS TRADITIONAL TO COLLECT SECTIONS ON THE DULL SIDE OF THE GRIDS.


It works well for us here, and is used routinely every day. Good luck,

Gib

}
} Happy holidays oh great wise ones of microscopy!
}
} (Apologies to John Lennon)
}
}
} Imagine all day sectioning,
} it isn't hard to do.
} Putting sections on bare 200 mesh grids
} and heat fixing under a light to boot.
}
} Imagine all the sections coming off
} while you do the stain.
} Oh oh
} You have to figure out what's wrong,
} while racking your brain.
} If you all can help,
} you'll help me from going insane.
}
}
} Any suggestions will be greatly appreciated. Thanks is advance!
}
} Paula :-)
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Fri Dec 8 15:45:10 2000



From: Erickson, Lauren -Belle Plaine :      lrerickson-at-imcglobal.com
Date: Fri, 8 Dec 2000 15:26:41 -0600
Subject: SEM of Potash

Contents Retrieved from Microscopy Listserver Archives
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I'm looking for methods of preparing compacted potassium chloride fertilizer
for the SEM. We believe that grain size is indicative of compaction quality
in our process, however we're not sure of the best way to prepare the
samples. The compactor flake is approximately 2"x1"x0.25". Any advice
would be much appreciated.

Thanks,
Lauren Erickson
lrerickson-at-imcglobal.com


From daemon Fri Dec 8 17:18:22 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 8 Dec 2000 17:12:26 -0600
Subject: Wanted Wollaston prisms for Reichert Zetopan DIC scope

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I have a freind that need then main prism slide containing the Wollaston
prism for Reichert Zetopan DIC scope. This is the slide going in the upper
body of the scope. He also needs the polorizor that fits over the light
soruce but that is of much less importance beause it can be easily made.
While the prism is constructed of unobtainium.

He is willing to pay a reasonable price for the part.

If any of you have a spare or left over you could make a very dissapointed
man a lot happier.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Fri Dec 8 17:18:27 2000



From: sghoshro-at-nmsu.edu
Date: Fri, 8 Dec 2000 16:11:21 -0700 (MST)
Subject: Re: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
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Paula,

I have noticed that in some cases the kind of grid you use is quite
important. I had similar problems with Gilder grids when using Spurr
embedded plant tissue sections. Later I switched to Veco grids, the
problem went away.

Good luck,

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Fri Dec 8 18:12:07 2000



From: JNunnari-at-aol.com
Date: Fri, 8 Dec 2000 19:08:51 EST
Subject: re:endothelial junctions

Contents Retrieved from Microscopy Listserver Archives
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Sandy Hancock
Try the following reference;
Zand, Underwood, Nunnari, Majno and Joris,
Endothelium and 'silver lines'. An electron microscopic study. 1982 Virchows
Archiv of Path Anat. 395: 133-144
Good luck
John


From daemon Fri Dec 8 19:03:23 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 8 Dec 2000 18:58:20 -0600
Subject: Re: Objectives for AO microscope

Contents Retrieved from Microscopy Listserver Archives
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Thanks to everyone for the response. It will take me a little while to
fully sort through everything, but I appreciate the help.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals

On Fri, 8 Dec 2000 02:31:47 -0800 (PST), Ken Tiekotter wrote:

} Dear Roger,
}
} I do not know if I can help, but I have a an excellent 50x oil AO
} objective with a correction collar used on an AO MicroStar series
compound
} microscope. This is the scope with a grey finish. The numerical aperture
} of this objective is 0.80 and with the correction collar exceeds the
light
} gathering capacity and resolution of any dry 40x or 63x dry objective.
}
} Do you have any items you can perhaps trade, i.e., Zeiss objective
lenses?
}
} Let me know what you think.
}
} Ken
} -------------
} Ken Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N. Willamette Blvd.
} Portland, OR 97203
}
} Contact tel. number: 503-413-5391
}
} On Thu, 7 Dec 2000, Roger Moretz wrote:
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } Greetings:
} }
} } I have an old American Optical Spencer microscope (rescued from a trash
} } bin). It is a binoc, three objective turret (with 4x, 10x, 20x) and
} } built-in light source scope. I have loaned the scope to a young man
who is
} } being home schooled, and he has become really interested in microscopy,
and
} } aquatic biology. He is at the point that he needs at least a 40x
objective,
} } and all I have are the 3 that came with the scope. The only numbers I
can
} } find on the scope are 1063-1AA and 1235, and neither is specifically
} } designated as the model or serial numbers. Can anyone direct me to a
source
} } for objectives for this type of microscope? These old scopes used a
} } standard thread and tube length, so maybe there are non-manufacturer
} } objectives that I can put on the scope. I haven't been able to look
thru an
} } Edmund Scientific catalog (there's one around here somewhere), but I
would
} } hope that either a collector or someone at Leica could provide some
} } additional info.
} }
} } Thanks in advance.
} }
} } Roger Moretz, Ph.D.
} } Dept of Toxicology
} } Boehringer Ingelheim Pharmaceuticals
} }
} }
} }
} }
} }
} } _______________________________________________________
} } Tired of slow Internet? Get -at-Home Broadband Internet
} } http://www.home.com/xinbox/signup.html
} }
} }
} }
}





_______________________________________________________
Tired of slow Internet? Get -at-Home Broadband Internet
http://www.home.com/xinbox/signup.html




From daemon Fri Dec 8 19:30:42 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Fri, 8 Dec 2000 19:27:56 -0600
Subject: Re: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
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Paula
Try this cleaning procedure for your copper grids:
1) Place your grids in a clean beaker, 100ml size will do.
2) Cover the gird with Glacial Acetic Acid, approximately 20cc (the idea
here is to get the grim and grease
off the grids not to eat the metal away)
3) Sonicate the grids in this acid for about 5 to 10 minutes.
4) Rinse in 100% acetone until the smell of the acetic acid is gone.
5) Do a final rinse in 100% ETOH (denatured is ok) and pipette the ETOH off
and invert the beaker into a glass petri dish that has a clean whatman
filter paper in it and place in your 60 degree over for an hour or more. The
grids will fall on to the filter paper. Cover the bottom of the glass with a
top.
The grids will keep without father cleaning until they are all used up. I
use a glass petri dish because there is less static charge with glass.

Ron Austin
Dept. of Pathology
LSU Medical Center
Shreveport, LA
rla-at-mindspring.com
318-675-4557



From daemon Fri Dec 8 19:41:16 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 08 Dec 2000 17:38:09 -0800
Subject: Re: SEM of Potash

Contents Retrieved from Microscopy Listserver Archives
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I'd just try a simple first shot approach of putting some of
the particles on a sticky tab, and coating it with about
60A of Au/Pd. Then, take a look at it under the SEM.
Based on this imaging, other approaches may or may
not be necessary.

It may or may not make a difference relative to the
type of SEM that you are using (FESEM vs. thermionic).
I would think that these are rather large specimens.
Either system should do a nice job.

gary g.


At 01:26 PM 12/8/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Dec 9 09:10:03 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Sat, 9 Dec 2000 14:59:05 +0000 (GMT Standard Time)
Subject: Flying grids and sticking stubs

Contents Retrieved from Microscopy Listserver Archives
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} From time to time, someone opens a grid storage box in our lab, and
because of the static that has built up on the plastic, the grids fly out
and are randomized. We have a Zerostat gun next to the TEM, but our
laboratory/office space is fragmented worse than a SWAP file, and one
can't simply reach for the ion gun every time one opens a grid box. Does
anyone (a) have any simple solutions to this problem (b) know of a
supplier of grid boxes made of a different plastic that doesn't charge up
so horribly?

In the longer term, would there be scope for making grid boxes out of a
plastic loaded with a filling to make it conductive enough for charges to
slowly leak away, so avoiding the problem altogether?

And on a similar theme, does anyone know of storage boxes for SEM stubs
into which the stubs can fit easily, i.e. not having to be rammed in?

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+






From daemon Sat Dec 9 12:23:01 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 09 Dec 2000 22:01:46 -0500
Subject: TEM and SEM storage box problems

Contents Retrieved from Microscopy Listserver Archives
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Antistatic sprays are available from electronic components suppliers
such as Farnell and RS Components. These are mainly intended to
de-static the plastic covers of ammeters, etc. to stop them
influencing the readings.

You could also try coating the interior surfaces of your box with a
thin layer of carbon or gold next time you have the coater running.

Chris



Date sent: Sat, 9 Dec 2000 14:59:05 +0000 (GMT Standard Time)
} From: "Robert H. Olley" {r.h.olley-at-reading.ac.uk}
To: Microscopy Newsgroup {Microscopy-at-sparc5.microscopy.com}
Copies to: # {r.h.olley-at-reading.ac.uk}


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Robert Olley wrote:
================================================================
} From time to time, someone opens a grid storage box in our lab, and because
of the static that has built up on the plastic, the grids fly out and are
randomized. We have a Zerostat gun next to the TEM, but our
laboratory/office space is fragmented worse than a SWAP file, and one can't
simply reach for the ion gun every time one opens a grid box. Does anyone
(a) have any simple solutions to this problem (b) know of a supplier of grid
boxes made of a different plastic that doesn't charge up so horribly?

In the longer term, would there be scope for making grid boxes out of a
plastic loaded with a filling to make it conductive enough for charges to
slowly leak away, so avoiding the problem altogether?

And on a similar theme, does anyone know of storage boxes for SEM stubs into
which the stubs can fit easily, i.e. not having to be rammed in?
=================================================================
There are a number of grid boxes being molded today, some of which are being
molded with plastics that have had added antistatic additives. The SPI
Slide-A-Grid™ storage boxes (see website below) are molded with such
antistatic additives. This same box is offered (but under other names) by
some of the other leading firms offering these kinds of products to EM users
. However, under certain conditions, apparently humidity related, even
these boxes reportedly can develop a charge.

For SEM storage boxes, there are two approaches to the "base plate" that
sits in the bottom of each box, the first being a "hard" plastic such as
polypropylene and the other being with a "soft" plastic, specifically an
elastomeric plastic. SPI Supplies has offered storage boxes with the soft
plastic for some number of years, the advantage being that the mounts do fit
in easily and do not have to be "rammed" in. I do not know of anyone else
who is molding mounting plates out of the soft "elastomeric plastic" (but of
course I could be wrong about that).

Note: One of the biggest sources of misfit between the storage boxes and
the 3/8" (9.5 mm) round mounts is that boxes molded in the USA tend to be
made for 3/8" diameter mounts and boxes molded elsewhere seem to be made for
10 mm diameter mounts. The problem arises when one tries to put a 10 mm
round mount into a box designed for the 3/8"round mounts. If the "ramming"
problem you described could be this kind of a problem, a mixing up of
boxes/mounts might be the reason for the described difficulty. The flip-
side of this problem is when 3/8" mounts are put into boxes molded in Europe
to take 10 mm round mounts, and the result is, the mounts fall out when the
box is tipped.

Disclaimer: SPI Supplies offers boxes for the storage of both TEM grids and
SEM mounts, the details of which can be found on our website below.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================








From daemon Sun Dec 10 03:20:44 2000



From: Trevor Sewell :      sewell-at-uctvms.uct.ac.za
Date: Sun, 10 Dec 2000 10:16:12 +0200
Subject: EM position vacant in Cape Town

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UNIVERSITY OF CAPE TOWN

TECHNICAL OFFICER/SENIOR TECHNICAL OFFICER/CHIEF TECHNICAL OFFICER
ELECTRON MICROSCOPE UNIT

If you have experience in electron microscopy and the use of
computers, then we invite you to apply for this post in a fast-moving
and ever-changing environment geared to servicing research needs.

The Electron Microscope Unit (EMU) primarily serves researchers in the
faculties of Science, Engineering and the Built Environment and Health
Sciences at UCT. The EMU aims to be the foremost provider of such
services in the Western Cape. Currently, the Unit has two scanning
and two transmission electron microscopes as well as a number of light
microscopes and will take delivery of a cryo-TEM early next year.

Duties of the appointee will include instrument operation, sample
preparation, user training and laboratory management.

The level of appointment will depend on the qualifications and the
experience of the successful candidate. Therefore, the remuneration
package, including benefits, is negotiable between R75 000 - R156 100
a year.

Please send a letter of application plus your CV (including the names,
postal/email addresses, telephone/fax numbers of 2 referees) to
Associate Professor Trevor Sewell, Director: Electron Microscope Unit,
UCT, Rondebosch, 7701 by 8 January 2001; tel: (021) 650-2817; fax:
689-1528; email: sewell-at-uctvms.uct.ac.za



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5="YA8RYZ82]D97!T {R]E;74-"-at-T*
`
end



From daemon Sun Dec 10 10:16:33 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Sun, 10 Dec 2000 09:58:34 -0600
Subject: RE: Flying grids and sticking stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


During my laboratory days I never worked in a room were static was that bad -
and that included Canadian winters. Carpet and poor humidification can make a
static problem worse.
I never liked the boxes with a sliding lid for another reason: If they dropped
when open from a modest height several grids may escape and that is really the
same problem.
Probably all of the EM suppliers carry grids boxes that hold 24 grids and have
a rotating lid with two apertures. Only one grid at a time is can to escape.
Presto: No more feral grids.

I'm sure that "smarter" stub boxes could be designed, but would people buy
them
at 2 or 3 times the price?
We (and others) have a single SEM mount Storage/mailer and this is much better
for holding pin type mounts.
Wouldn't is be lovely if all EM manufacturers used the same (I vote for pin
type) mounts.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Sunday, December 10, 2000 12:59 AM, Robert H. Olley
[SMTP:r.h.olley-at-reading.ac.uk] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} } From time to time, someone opens a grid storage box in our lab, and
} because of the static that has built up on the plastic, the grids fly out
} and are randomized. We have a Zerostat gun next to the TEM, but our
} laboratory/office space is fragmented worse than a SWAP file, and one
} can't simply reach for the ion gun every time one opens a grid box. Does
} anyone (a) have any simple solutions to this problem (b) know of a
} supplier of grid boxes made of a different plastic that doesn't charge up
} so horribly?
}
} In the longer term, would there be scope for making grid boxes out of a
} plastic loaded with a filling to make it conductive enough for charges to
} slowly leak away, so avoiding the problem altogether?
}
} And on a similar theme, does anyone know of storage boxes for SEM stubs
} into which the stubs can fit easily, i.e. not having to be rammed in?
}
} +------------------------------------------------------------------------+
} | Robert H.Olley Phone: |
} | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
} | University of Reading {University internal extension 7867 |
} | Whiteknights Fax +44 (0) 118 9750203 |
} | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
} | England URL: http://www.reading.ac.uk/~spsolley |
} +------------------------------------------------------------------------+
}
}
}
}




From daemon Sun Dec 10 11:06:58 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 10 Dec 2000 10:58:03 -0600
Subject: M&M 2001 Meeting August 5-9 , 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

On behalf of the Microscopy Society of America and the Microbeam Analysis
Society,
we invite you to attend Microscopy and Microanalysis 2001, August 5-9, in
Long Beach,
California. The program chair, Bob Price, and Co-Chairs Edgar Voelkl (MSA) and
Inga Holl Musselman (MAS), have assembled another excellent technical program
that continues the tradition of exciting and current presentations at the
meetings.
An outstanding list of invited speakers highlights the symposia in a
number of key
areas. Special symposia this year again cover a wide range, including
"Microscopy
and Microanalysis in the Real World," a special biological symposium honoring
Dr. Inou*, and "Atom Location by Channeling Enhancement of X-Ray and EELS
Signals"
among others, plus a wide variety of applications in biology and materials
research.

Both invited and contributed abstracts are critical to the success of these
symposia,
so we urge you to submit your work. Remember that the abstract deadline is
February 15th, 2001.

A special Pre-meeting Congress, "Imaging Life: From Cells to Whole
Animals," as well
as several workshops and short courses on important, basic techniques will
precede
the meeting. Special symposia, tutorials and presentations sponsored by the
Technologists'
Forum, the MSA Education Committee, and various commercial exhibitors will
be held
during the course of the meeting. There are also Presidential Happenings,
ceremonies
for Award Winners, and the world's largest display of microscopes and
related equipment.

Our Local Arrangements Committee, headed by Robert Koch, has arranged an
excellent
venue at the Long Beach Convention Center for both the scientific sessions
and the exhibits.
They have also arranged a memorable program of social events including a
Sunday night
reception on the Queen Mary, the annual golf tournament, a harbor cruise,
and a large
number of options for enjoying the city and the surrounding area (don't
forget the theme parks!).
With its rich history, wealth of educational institutions and active
industry, Long Beach
provides a wonderful context for this meeting, and we urge you to come to
learn, teach,
share and above all to enjoy. Whatever your connection to microscopy, we
look forward
to welcoming you to Long Beach in August at Microscopy and Microanalysis 2001.

Ron Anderson, President
Microscopy Society of America


Richard Linton, President
Microbeam Analysis Society

--------------------------

The program is as usual extensive and detailed information can be found
on the WWW page.

http://www.msa.microscopy.com/MSAMeetings/MM01/MMHomePage.html



Cheers....
Nestor
Your Friendly Neighborhood SysOp.

-------------------------





From daemon Sun Dec 10 18:38:59 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 10 Dec 2000 17:56:16 -0600
Subject: Administrivia: November Archives On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The Microscopy Listserver Archives from November are
now on-line.

http://www.msa.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Nestor
Your Friendly Neighborhood SysOp




From daemon Sun Dec 10 18:39:00 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 10 Dec 2000 18:25:32 -0600
Subject: Administrivia: November Archives On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The Microscopy Listserver Archives from November are
now on-line.

http://www.msa.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Nestor
Your Friendly Neighborhood SysOp
6:25 PM CST 12/10/00




From daemon Sun Dec 10 19:34:22 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 10 Dec 2000 19:24:41 -0600
Subject: Re: Flying grids and sticking stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have fought static problems in wide variety of problems and three things
help. Grounding with metal, raising the humidity to 50% and spraying the
area or items with antistatic products designed for laundry. Downy was the
one I used. Temporary relief can be had by spraying the area with water
from Windex like spray bottle. This only last for a few minutes to an hour
at most.

I am sure fabric antistatic spray would not be acceptable in many places
in a lab. But floors and tables might benifit from them.

Spraying carpets with antistatic spray once a month work for us in 15 to
20% humidity.

Your mileage my vary.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

} From: "Jim at ProSciTech" {jim-at-proscitech.com}
} During my laboratory days I never worked in a room were static was that
bad -
} and that included Canadian winters. Carpet and poor humidification can
make a
} static problem worse.
} I never liked the boxes with a sliding lid for another reason: If they
dropped
} when open from a modest height several grids may escape and that is
really the
} same problem.
} Probably all of the EM suppliers carry grids boxes that hold 24 grids
and have
} a rotating lid with two apertures. Only one grid at a time is can to
escape.
} Presto: No more feral grids.
}
} I'm sure that "smarter" stub boxes could be designed, but would people
buy
} them
} at 2 or 3 times the price?
} We (and others) have a single SEM mount Storage/mailer and this is much
better
} for holding pin type mounts.
} Wouldn't is be lovely if all EM manufacturers used the same (I vote for
pin
} type) mounts.
} Cheers
} }
} } } From time to time, someone opens a grid storage box in our lab, and
} } because of the static that has built up on the plastic, the grids fly
out
} } and are randomized. We have a Zerostat gun next to the TEM, but our
} } laboratory/office space is fragmented worse than a SWAP file, and one
} } can't simply reach for the ion gun every time one opens a grid box.
Does
} } anyone (a) have any simple solutions to this problem (b) know of a
} } supplier of grid boxes made of a different plastic that doesn't charge
up
} } so horribly?
} }
} } In the longer term, would there be scope for making grid boxes out of
a
} } plastic loaded with a filling to make it conductive enough for charges
to
} } slowly leak away, so avoiding the problem altogether?
} }
} } And on a similar theme, does anyone know of storage boxes for SEM
stubs
} } into which the stubs can fit easily, i.e. not having to be rammed in?
} }
}
}




From daemon Mon Dec 11 06:21:42 2000



From: John.Catino-at-mineralstech.com
Date: Mon, 11 Dec 2000 07:13:01 -0500
Subject: Kevex Quantum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kevex Quantum EDS system with Sesame drive is available for no charge for
pick-up in Easton, PA. The detector is NOT included. System has Syquest
drives and monitor is in need of repair.




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From daemon Mon Dec 11 11:12:32 2000



From: mark brady :      brady-at-MMM.COM
Date: Mon, 11 Dec 2000 11:04:52 -0600
Subject: Counting and mounting media

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am doing some bacterial counting work. My basic method
involves taking some bacteria in a measured quantity of
suspension and running it thru an anodisc (aluminum oxide)
filter having .2 micron pores.

My problem is this: when mounting the filter on a slide, I find
that the disc is almost dry before I can mount it. I have tried
adding water and or oil below and above the filter. Water
sometimes works but it is a very tricky matter because the
water may redistribute the bacteria when applied. This affects
my sampling since the bacteria may no longer be uniform.

The same problem occurs with oil. Furthermore, the oil, if
applied to a filter with some water remaining, gives a blurry
image, perhaps due to the oil-water mix.

I am also concerned with air bubbles. Is there a method by
which one can prevent air bubbles under, over and in the
pores of the filter?

-Mark




From daemon Mon Dec 11 11:18:48 2000



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Mon, 11 Dec 2000 12:14:25 -0500
Subject: Re: Flying grids and sticking stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would *STRONGLY* recommend against using any anti-static spray on grid
boxes themselves unless you are certain of the components of the spray. I
remember one batch of grid boxes we had to throw away because the
formulation of the plastic mix during manufactur was evidently wrong, and
we got horrendous contamination of samples which had been stored in the
boxes. We don't even touch the tweezers we are going to use for our
samples with our bare hands! I would never spray some unknown material
intended for some other, much less critical (in terms of cleanliness)
application, anywhere near anything related to the samples themselves.

Tony G-R


}
} Antistatic sprays are available from electronic components suppliers
} such as Farnell and RS Components. These are mainly intended to
} de-static the plastic covers of ammeters, etc. to stop them
} influencing the readings.
}
} You could also try coating the interior surfaces of your box with a
} thin layer of carbon or gold next time you have the coater running.
}
} Chris
}
}

} }
} } } From time to time, someone opens a grid storage box in our lab, and
} } because of the static that has built up on the plastic, the grids fly out
} } and are randomized. We have a Zerostat gun next to the TEM, but our
} } laboratory/office space is fragmented worse than a SWAP file, and one
} } can't simply reach for the ion gun every time one opens a grid box. Does
} } anyone (a) have any simple solutions to this problem (b) know of a
} } supplier of grid boxes made of a different plastic that doesn't charge up
} } so horribly?
} }
} } In the longer term, would there be scope for making grid boxes out of a
} } plastic loaded with a filling to make it conductive enough for charges to
} } slowly leak away, so avoiding the problem altogether?
} }
} } And on a similar theme, does anyone know of storage boxes for SEM stubs
} } into which the stubs can fit easily, i.e. not having to be rammed in?
} }


* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*




From daemon Mon Dec 11 11:35:30 2000



From: Max Sidorov :      msv-at-nl.feico.com
Date: Mon, 11 Dec 2000 18:27:52 +0100
Subject: TEM - Update again (problems with accessing): Contrast Transfer Function Freeware (ctfExplorer)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:

Apparently, Bravenet (who provides a quick link url for me) is down for
updates (at least that's what they say).
The quick link (http://clik.to/ctfexplorer) should start working again
shortly.
In a meantime you can use a direct link: http://www.ctfexplorer.f2s.com

Hope this helps,
Max S

-------------------
Dear All:

ctfExplorer has been updated.
New features/improvements:
1. Now it is possible to export graphs to text (tab-delimited).
2. List-tree with the microscopes is wider now.
3. Tab stops in dialogs arranged in a logical sequence.
4. Now it calculates the Lichte-defocus. (Useful for holography).
5. Additional slider-control is added: allows to change "magnification",
i.e. convergence. Just to show people why it is not always a good idea to
work at 1,000,000x and why some people do high resolution work at as low as
50,000x magnification.

Here is the link: http://clik.to/ctfexplorer (always use this link. it will
direct you to the right location).

Enjoy,

Max Sidorov
---
(until December 31 2000)
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com (mailto:maxsidorov-at-bigfoot.com)

----------Additional Info----------
Dear All:
I wrote a piece of software which I believe would be of interest to the
microscopy community.
It's a highly interactive program which calculates/displays the contrast
transfer function of TEMs. I know that there are similar programs floating
around but ctfExplorer does not only 1d but also 2d calculations/display
with 2-fold and 3-fold astigmatism imposed. There are other unique features
to it. All parameters (defocus, voltage, Cs, etc) can be changed
interactively.

DISCLAIMER: I do work for FEI/Philips Electron Optics. However, this
software is not an official product of FEI and FEI is not responsible for
its distribution/support. This software is freeware.

To the best of my knowledge, the software does not have any nasty bugs. It
is tested under Windows 95/98 and Windows NT4.

Please give it a try. Please direct your suggestions and comments to
maxsidorov-at-bigfoot.com
I hope you'll find the software useful.

Here is the link: http://clik.to/ctfexplorer

Enjoy,

Max Sidorov
---
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com





From daemon Mon Dec 11 11:45:24 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 11 Dec 2000 11:41:52 -0600
Subject: MSA Undergraduate Research Scholarship Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Society of America
Undergraduate Research Scholarship Program

With this year's call for applications the MSA Undergraduate Research
Scholarship Program begins its 13th year providing funding for
undergraduate research. To date over 65 projects covering a wide
range of topics in the physical and biological sciences have received
support through this program. Over the years nearly all the
scholarship recipients have maintained a strong interest in imaging
sciences and have gone on to graduate school, professional school,
teaching, or industry positions.

The program, which is funded by MSA, is able to support approximately
30% to 40% of applicants. The maximal award from MSA is currently
$3000 and helps to provide student stipends, supply costs, and
limited travel expenses associated with the research. Additional
support in the form of instrument use time, equipment purchases, etc.
is generally provided by the studentís supervisor and/or through the
sponsoring institution. Abstracts reporting the research results,
are prepared by scholarship awardees, and published in "Microscopy
and Microanalysis".

The program actively seeks external sources of matching funds in
order to maintain the favorable levels of support both in terms of
the number of projects supported and the level of support for each.
We are extremely grateful for the matching support provided by MSA
sustaining members and individuals. Their support has enabled the
program to increase both the number of awards and the maximum amount
of each award to $3000.

The MSA Undergraduate Research Scholarship Program is currently
soliciting applications from students interested in conducting a
research project which involves the use of any microscopy technique.
Students must be sponsored by a member of MSA. The maximal award is
$3000.

This is not limited to students in the USA or North America. Any
undergraduate student anywhere is eligible to apply for the award,
provided the person supervising their research is a member of MSA.

**The application deadline is Dec 31, 2000.**

Applications can be obtained from the MSA Business Office,
businessoffice-at-msa.microscopy.com or call toll free at (800) 538-3672.

If you have any questions or require additional information regarding
the program please contact either:

Dr. Ralph Albrecht, University of Wisconsin
1656 Linden Drive, Madison, WI 53706
(608) 263-3952 or 263-4162; (608) 262-7420 FAX;
albrecht-at-ahabs.wisc.edu

Dr. Richard Ornberg, Monsanto Company
Analytical Sciences Center, 800 N. Lindbergh Blvd., St. Louis, MO 63167
(314) 694-1184; (314) 694-6727 FAX;
rlornb-at-ccmail.monsanto.com


From daemon Mon Dec 11 12:33:12 2000



From: Paul Anderson :      paanders-at-lynx.dac.neu.edu
Date: Mon, 11 Dec 2000 13:26:12 -0500 (EST)
Subject: JEOL 100CXII

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FOR SALE:
1988 JEOL 100CXII with (+/-) 45degree tilt stage, dual sample holder,
and Gatan camera system: a rugged, reliable transmission electron
microscope in perfect working order, with water chiller: $30,000.
Buyer is responsible for take-down, shipping, and set-up costs. A Kevex Delta
I Class EDS analysis unit is also included, but currently not installed.

Feel free to come by for a test drive!
317 Egan Research Center
Northeastern University
Boston, MA 02115
For directions and to set up an appointment, call (617) 373-5909.

If interested in purchasing, please contact:
Professor Terry K. Baker
(617) 373-2123




From daemon Mon Dec 11 13:23:55 2000



From: Drew R. Van Orden :      drew-at-rjlg.com
Date: Mon, 11 Dec 2000 14:18:39 -0500
Subject: Please Post - Position Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position Opening
Materials Scientist

RJ Lee Group's Bay Area Facility, a materials characterization and
consulting laboratory, is seeking a motivated individual to actively manage
projects related to the evaluation of materials and processes used in the
electronics industry. Job applicants should have an MS or equivalent in
physics, materials science or related discipline, at least two years of
relevant work experience, and be amenable to working in a hands-on and
fast-paced team environment. A working knowledge of the application and
theory associated with sample preparation methods and corresponding
analytical techniques such as optical microscopy, scanning electron
microscopy (SEM), transmission electron microscopy (TEM), Auger spectroscopy
and other surface analysis methods is critical. Strong oral and written
communication skills are a must. Job duties would involve, but not be
limited to: materials consulting, developing / evaluating analytical
approaches to constructively investigate problems, and presenting laboratory
results to a variety of technical as well as non-technical personnel.
Salary is commensurate with experience. Interested candidates should
forward qualifications along with salary history to: RJ Lee Group, Inc.,
Attn. Human Resources, 350 Hochberg Road, Monroeville, PA 15146.

RJ Lee Group is an Equal Opportunity Employer



Drew R. Van Orden, PE
RJ Lee Group, Inc.
350 Hochberg Road
Monroeville, PA 15146
(724) 387-1869
drew-at-rjlg.com





From daemon Mon Dec 11 14:43:56 2000



From: Marilyn Howton :      mhowton-at-hsc.wvu.edu
Date: Mon, 11 Dec 2000 15:38:37 -0500
Subject: EM on cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am new to this group, so if this is question has been covered, please excuse.
I usually do TEM on tissue from biopsies, etc., and now someone wants EM on cells grown in culture. I have done this for intact monolayers (very tedious), but these would be on trypsined-off and pelleted cells. I need to know if there is an easy way to handle them as an entire pellet, so I don't have to cfg. after each fixation/dehydration step, and so they will be compacted for sectioning.
Thanks in advance for any advice.
Marilyn Howton, Pathology Dept. West Virginia University



From daemon Mon Dec 11 15:49:32 2000



From: Vr. Richard Bejsak-Collorado-Mansfeld :      ricardo-at-ans.com.au
Date: Tue, 12 Dec 2000 08:48:31 +1100
Subject: Mounting media

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

I would like to apologise for my humble trivial request to all highly
qualified expert like you.
I am running website www.coleoptera.org about beetles and I just open new
section FAQ with info about collecting, preparation, techniques etc.

I just like to add section about mounting small insects into permanent
slide.
Is there anything so good as Canadian balsam?

Next question will be how to best prepare insects for SEM..

Any help deeply appreciated.

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Collorado-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).




From daemon Mon Dec 11 16:03:34 2000



From: alineves-at-unisys.com.br
Date: Mon, 11 Dec 2000 15:59:23 -0600
Subject: Ask-A-Microscopist: Retardation of a specimen in polarized light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(alineves-at-unisys.com.br) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, December
11, 2000 at 14:50:32
---------------------------------------------------------------------------

Email: alineves-at-unisys.com.br
Name: Aline Neves
School: Federal University of Rio de Janeiro
State: Rio de Janeiro

Question: How do I measure the retardation of a specimen in polarized light
microscopy. I have the Berek conpensator to achive a quantitative measure,
but exactly how do I do it? Where can I get more information about that?

---------------------------------------------------------------------------




From daemon Mon Dec 11 17:03:03 2000



From: sandeep GUPTA :      sundep-at-yahoo.com
Date: Mon, 11 Dec 2000 14:58:16 -0800 (PST)
Subject: Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
I insect morphology and use Nikon SMZ-2T binocular
stereomicroscope to study the various insect body
parts. I use micrometer inside the ocular lens to
take
measurements. I do not know how to calibrate these
measurements. It will be nice if if someone can refer
literature or material on internet for calibration
and
mathematical calculations.If my question is
incomplete
please let meknow so that i can provide more
nformation.
sandeep gupta


__________________________________________________
Do You Yahoo!?
Yahoo! Shopping - Thousands of Stores. Millions of Products.
http://shopping.yahoo.com/


From daemon Tue Dec 12 03:00:47 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Tue, 12 Dec 2000 08:42:07 -0000
Subject: Re: EM on cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Marilyn,

I am working with human cell lines in suspension and process the
cells in a pellet.

After the usual problems and much help from this list I worked out
a procedure which works for me.

It is critical that the pellet is small - in my experience not more
than 250 000 cells / pellet. This means you will not or hardly see
the pelleted cells until they have been in OsO4. All the dehydration
steps and infiltration steps are performed on a rotary shaker.

Here it is:

I transfer the cell suspension into conical BEEM capsules and spin
them in home made adapters at 900 x g. (It is essential to use a
swing bucket rotor, otherwise your cells smear along the sides of
the container and float off during the dehydration steps.)

After carefully removing the supernatant with fine tipped mini
pastetts I overlay the pellet with fixative, remove it again with
pastetts and so on. As soon as the OSO4 stains the pellet one
can see it and that makes the handling easier.
I use glass pipetts with OsO4 and propylenoxide and the resin-
propylen mixtures.

I usually infiltrate twice with epon-propylenoxide (1+2 and 1+1
respectively) for 45 minutes, then twice with pure resin. The tips of
glass Pasteur pipetts have to be cut to widen the opening
otherwise it is very difficult to remove the very viscous resin.

For sectioning I carefully flatten the very fine tip of the block with a
razor blade. The cells are evenly distributed, there is no need to cut
away surplus plastic. Just shape the block face to a trapezoid and
it can go straight into the ultramicrotome.

Good luck - if you require more details feel free to ask.

Regards
Claudia

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk


From daemon Tue Dec 12 04:26:45 2000



From: Carole Elleman :      c.j.elleman-at-reading.ac.uk
Date: Tue, 12 Dec 2000 10:20:23 -0000
Subject: TEM cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Marylyn Howton - one approach to fixing cells that have been stripped
off is to spin them into a pellet(500rpm) and then take up the pellet in 2%
agar - you can then take the pellet through deydration and embedding and all
the cells remain in a pellet. You can fix the cells in the pellet but that
is rather time consuming and it may be best to do the fixation steps first -
spinning down after each and then make into a pellet.
hope this helps
Carole Elleman



From daemon Tue Dec 12 07:25:21 2000



From: joachim.prutsch-at-leica-microsystems.com
Date: Tue, 12 Dec 2000 14:17:18 +0100
Subject: RE: Wanted Wollaston prisms for Reichert Zetopan DIC scope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gordon,


your freind could try to contact Mr. Rasche in Germany:
email: mikrovid-at-gmx.de
homepage: www.mikrovid.com

best wishes, Joachim


Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A-1170 Vienna Tel. +43 1 48899 - 235
Austria Fax +43 1 48899 - 350
---------------------- Weitergeleitet von Joachim
Prutsch/AUVIE/LMSCentral/Leica am 12.12.2000 14:11
---------------------------


"Gordon Couger" {gcouger-at-couger.com} am 09.12.2000 00:12:26



An: microscopy-at-sparc5.microscopy.com

Kopie: (Blindkopie: Joachim
Prutsch/AUVIE/LMSCentral/Leica)



Thema: Wanted Wollaston prisms for Reichert Zetopan DIC
scope






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have a freind that need then main prism slide containing the Wollaston
prism for Reichert Zetopan DIC scope. This is the slide going in the upper
body of the scope. He also needs the polorizor that fits over the light
soruce but that is of much less importance beause it can be easily made.
While the prism is constructed of unobtainium.

He is willing to pay a reasonable price for the part.

If any of you have a spare or left over you could make a very dissapointed
man a lot happier.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00







From daemon Tue Dec 12 07:59:43 2000



From: Ed Calomeni :      ecalomeni-at-mco.edu
Date: Tue, 12 Dec 2000 08:55:23 -0500
Subject: Re: EM on cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Marilyn,

Since you work in a hospital setting, get some unneeded serum from your clinical lab, pellet the sample cells, add 50-100 ul of serum to pellet, mix well
pellet cells again and then overlay with 3% glut. Next day, remove plug of cells/serum, cut into em size tissue pieces and process as you normally would.

Best of Luck,

Ed

Edward P. Calomeni

Medical College of Ohio
Department of Pathology
3000 Arlington Ave.
Toledo, OH 43614

419-383-3484
419-383-3066 (fax)
ecalomeni-at-mco.edu

} } } "Marilyn Howton" {mhowton-at-hsc.wvu.edu} 12/11/00 03:38PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am new to this group, so if this is question has been covered, please excuse.
I usually do TEM on tissue from biopsies, etc., and now someone wants EM on cells grown in culture. I have done this for intact monolayers (very tedious), but these would be on trypsined-off and pelleted cells. I need to know if there is an easy way to handle them as an entire pellet, so I don't have to cfg. after each fixation/dehydration step, and so they will be compacted for sectioning.
Thanks in advance for any advice.
Marilyn Howton, Pathology Dept. West Virginia University





From daemon Tue Dec 12 08:10:25 2000



From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 05 Dec 2000 17:30:12 -0400 (EDT)
Subject: Ammonium Molybdate as Negative Stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Nafisa Ghori:
Ammonium Molybdate is especially useful to contrast membranes as in
vesicles or liposomes. It also can be titrated to neutral pH whereas
UA cannot.

Don Gantz
Boston University School of Medicine
Biophysics Dept.


From daemon Tue Dec 12 10:15:22 2000



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Tue, 12 Dec 2000 09:14:36 -0500
Subject: TEM contaminated?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues, a pathologist asked to use our Transmission Electron
Microscope(TEM) and when I was going to help him load his copper grids, he
said he would do it as it was a fecal preparation for viral studies. I
found out that material on formar coated copper grids was not fixed. After
he viewed (no photos taken) I pulled the copper grid holder and all items
touched and ultrasonicated with absolute ethanol and then acetone and then
placed items without touching in a 70oC oven for an hour. I feel that is
enough, but would like to know if anyone else has been in this predicament.
Thanks, Teresa




From daemon Tue Dec 12 10:18:41 2000



From: jo verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Tue, 12 Dec 2000 17:05:29 +0000
Subject: TEM image simulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a computer programm that simulates the contrast induced

by defects in crystals. The defects I am interested in are twins and
steps in the twin planes. Which programms are available in which you
can simulate many different types of defects and that allow to change
the parameters easily?

Best regards,
Jo



From daemon Tue Dec 12 10:40:10 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Tue, 12 Dec 2000 08:31:36 -0800
Subject: EM on cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Marilyn,
I concentrate the cells by a gentle spin, and embed them in a drop of 2% noble
agar. After hardening and trimming the blocks to a size of you usual biopsy you
can process them without loosing any. A}

Alice Dohnalkova
S&E Assoc., Dep. of Environmental Microbiology
Battelle, Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321


-----Original Message-----
} From: Marilyn Howton [SMTP:mhowton-at-hsc.wvu.edu]
Sent: Monday, December 11, 2000 12:39 PM
To: Microscopy-at-sparc5.microscopy.com


I am new to this group, so if this is question has been covered, please excuse.
I usually do TEM on tissue from biopsies, etc., and now someone wants EM on
cells grown in culture. I have done this for intact monolayers (very tedious),
but these would be on trypsined-off and pelleted cells. I need to know if there
is an easy way to handle them as an entire pellet, so I don't have to cfg. after
each fixation/dehydration step, and so they will be compacted for sectioning.
Thanks in advance for any advice.
Marilyn Howton, Pathology Dept. West Virginia University



From daemon Tue Dec 12 11:10:47 2000



From: reinhard rachel :      reinhard.rachel-at-biologie.uni-regensburg.de
Date: 12 Dec 2000 18:06:37 +0100
Subject: Re: EM on cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
"Re: EM on cell cultures" (Dec 12, 8:42am)
References: {3A35E4DF.17130.27D219-at-localhost}
X-Mailer: Z-Mail (4.0.1 13Jan97)
To: Claudia Hayward-Costa {LS_S562-at-crystal.kingston.ac.uk}

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I wonder if you have heard of the Hohenberg technique
described some 6 years ago in J.Microscopy; this
technique makes life so much easier for people working with single
cells in suspension, whether they are prokaryotes or eukaryotes.

H.Hohenberg could nicely demonstrate that the embedding and sectioning
is very much facilitated and structures are much better preserved when:
a) cells are taken up in cellulose capillaries, 200 micron in diameter;
b) cells are high-pressure frozen (as the very first step
for cryo-immobilization)
c) cells are freeze-substituted but not dehydrated at RT.
This technique has been used in the meanwhile by many other
people with success, ...
but still is not known to all working with single cells
in suspensions.

Hohenberg et al (1994) J. Microscopy 175, 34-43

have fun!
Reinhard Rachel

Dr. Reinhard Rachel
Universitaet Regensburg
Lehrstuhl fuer Mikrobiologie (Prof. Dr. K.O. Stetter)
D - 93040 Regensburg
Tel.: +49-941-943-4534
Fax.: +49-941-943-1824
e-mail: Reinhard.Rachel-at-biologie.uni-r.de


From daemon Tue Dec 12 12:15:53 2000



From: Carole Elleman :      c.j.elleman-at-reading.ac.uk
Date: Tue, 12 Dec 2000 10:20:23 -0000
Subject: TEM cell cultures

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Dear Marylyn Howton - one approach to fixing cells that have been stripped
off is to spin them into a pellet(500rpm) and then take up the pellet in 2%
agar - you can then take the pellet through deydration and embedding and all
the cells remain in a pellet. You can fix the cells in the pellet but that
is rather time consuming and it may be best to do the fixation steps first -
spinning down after each and then make into a pellet.
hope this helps
Carole Elleman






From daemon Tue Dec 12 12:23:33 2000



From: Ed Calomeni :      ecalomeni-at-mco.edu
Date: Tue, 12 Dec 2000 08:55:23 -0500
Subject: Re: EM on cell cultures

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Morning Marilyn,

Since you work in a hospital setting, get some unneeded serum from your clinical lab, pellet the sample cells, add 50-100 ul of serum to pellet, mix well
pellet cells again and then overlay with 3% glut. Next day, remove plug of cells/serum, cut into em size tissue pieces and process as you normally would.

Best of Luck,

Ed

Edward P. Calomeni

Medical College of Ohio
Department of Pathology
3000 Arlington Ave.
Toledo, OH 43614

419-383-3484
419-383-3066 (fax)
ecalomeni-at-mco.edu

} } } "Marilyn Howton" {mhowton-at-hsc.wvu.edu} 12/11/00 03:38PM } } }
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I am new to this group, so if this is question has been covered, please excuse.
I usually do TEM on tissue from biopsies, etc., and now someone wants EM on cells grown in culture. I have done this for intact monolayers (very tedious), but these would be on trypsined-off and pelleted cells. I need to know if there is an easy way to handle them as an entire pellet, so I don't have to cfg. after each fixation/dehydration step, and so they will be compacted for sectioning.
Thanks in advance for any advice.
Marilyn Howton, Pathology Dept. West Virginia University








From daemon Tue Dec 12 12:30:24 2000



From: sghoshro-at-nmsu.edu
Date: Tue, 12 Dec 2000 11:25:21 -0700 (MST)
Subject: EM on cell culture

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Marilyn,

You can use a tabletop airfuge with a very small rotor. Each tube holds
about 0.2 ml liquid. You can spin your sample for about an hour at a very
high speed and it will give you a very tight pellet. I used to spin the
sample in fixative and once you are done spinning, you can process the
pellet and do osmication, dehydration, infiltration and embedding.

Good luck,

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Tue Dec 12 12:46:34 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Tue, 12 Dec 2000 13:34:25 -0500
Subject: PROCESSING CELL SUSPENSIONS

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Hi:

I have been working with human red blood cells grown in cell culture that
are infected with Plasmodium falciparum for the past 9 years. The way I
handle them is to use normal fixatives (glut, OsO4, uranyl acetate) while
they are in suspensions and spin them down in-between steps. Following
fixation I spin them down in warm (45 degree C) 3% agarose (Sigma A5030
25gm, type 9 ultra low gelling temperature). When the agarose cools to room
temperature the pellet becomes like Jell-O in the tip of the 15ml falcon
tube. Then I simply cut off the tip and transfer the "glued together
pellet" to a glass vial and continue with dehydration and infiltration steps
as if I was handling a small hunk of tissue. Good luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Tue Dec 12 12:51:47 2000



From: DCiaburri-at-gdds.com
Date: Tue, 12 Dec 2000 13:44:03 -0500
Subject: RE: Wanted Wollaston prisms for Reichert Zetopan DIC scope

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Gordon,

Another person to try would be Dave Paschke, The Dawson Co.,
(617)484-7900. He had some parts for our Reichert MeF2 when I spoke to
him a year ago...you never know.

Diane Ciaburri
General Dynamics
Pittsfield, MA 01235
(413)494-2847





"joachim.prutsch-at-leica-microsystems.com"
12/12/00 08:17 AM


To: Microscopy-at-sparc5.microscopy.com
cc:
Subject: RE: Wanted Wollaston prisms for Reichert Zetopan DIC scope


Dear Gordon,


your freind could try to contact Mr. Rasche in Germany:
email: mikrovid-at-gmx.de
homepage: www.mikrovid.com

best wishes, Joachim


Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A-1170 Vienna Tel. +43 1 48899 - 235
Austria Fax +43 1 48899 - 350
---------------------- Weitergeleitet von Joachim
Prutsch/AUVIE/LMSCentral/Leica am 12.12.2000 14:11
---------------------------


"Gordon Couger" {gcouger-at-couger.com} am 09.12.2000 00:12:26



An: microscopy-at-sparc5.microscopy.com

Kopie: (Blindkopie: Joachim
Prutsch/AUVIE/LMSCentral/Leica)



Thema: Wanted Wollaston prisms for Reichert Zetopan DIC
scope


I have a freind that need then main prism slide containing the Wollaston
prism for Reichert Zetopan DIC scope. This is the slide going in the upper
body of the scope. He also needs the polorizor that fits over the light
soruce but that is of much less importance beause it can be easily made.
While the prism is constructed of unobtainium.

He is willing to pay a reasonable price for the part.

If any of you have a spare or left over you could make a very dissapointed
man a lot happier.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00











From daemon Tue Dec 12 12:57:07 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 12 Dec 00 11:01:35 -0800
Subject: RE: EM on cell cultures

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 12 Dec 00 11:01:35 -0800
Subject: RE: EM on cell cultures

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Reply to: RE: EM on cell cultures
Dear Marilyn,

A good way to prepare cell monolayers for EM by embedding them as a pellet is as follows:

Fix the cells on the substrate by adding double strength fixative to the culture medium. This will contain serum which is good for the next steps. Leave the fixative on the cells for about 2-5 min and then carefully scrape off the monolayer using a small scraper specially made for this from either a small piece of teflon or a soft wooden stick previously soaked in buffer. The cells will come off the substrate in either a single monolayer (if there are tight junctions present, or as a powdery suspension.
Quickly take the detached cells from the dish and centrifuge (I use full speed on the benchtop Eppendorf for about 2 seconds to get a pellet). If you do this without protein being present in the fixative (eg serum) the cells will not pellet but will stick to the sides of the tube (smearing). Once the cells have pelleted DO NOT resuspend them. Remove the fixative and medium and replace it with fresh fixative. Leave for the amount of time you want to fix for (eg 1hr). You will find that after the fixation is complete you will be able to remove the pellet from the bottom of the tube and treat it as if it was a piece of tissue. You should be able to cut it into smaller pieces for subsequent embedding. Follow your favorite protocol for this. I am sure that you will have pellets that do not fall apart. The secret is to fix as a pellet a.s.a.p. and then do not disturb until after fixation is complete. I think the fixative cross-links extracellular components of the cells together but this only happens if the cells are in close proximity as fixation occurs.

If you do prefer to process in the tube they were pelleted in, then a neat trick for speeding up all the steps is to centrifuge after each change of solution. You will see how quickly things go into the pellet if you centrifuge in the presence of osmium tetroxide. A 2 min centrifugation at top speed will cause even a large pellet to turn black all the way through. If you continue the processing in this tube then make sure the final infiltration in the resin is sufficient to go allthe way through the pellet. Be careful to remove all dehydrating solutions and propylene oxide from the tube. I tip them upside down for about half an hour before adding the final resin changes. You will soon realize if you did this successfully - a soft pellet will be the result of too much proylene oxide in the resin. I prefer to embed them after cutting into small pieces.

As they are a pellet of individual cells they will be much easier to embed in resin than a solid tissue piece. Any poor morphology you get will probably not be due to what you do them but what happens before you put them in fixative. Trypsin is not good for morphology, neither is transfection. Ccentrifugation or scraping prior to fixation will affect morphology too. It is a good rule to physically handle the cells as little as possible prior to fixation.

The biggest surprize is that they will have poor morphology if they have only been left to grow fror a day or two after passage. It is only after about three days that they really start to recover from this. Unfortunately, they will look really good in the light microscope so poor morphology will be your problem!

I hope this helps.

Paul Webster.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm

Marilyn Howton wrote:
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}
} I am new to this group, so if this is question has been covered, please excuse.
} I usually do TEM on tissue from biopsies, etc., and now someone wants EM on } cells grown in culture. I have done this for intact monolayers (very tedious), } but these would be on trypsined-off and pelleted cells. I need to know if there } is an easy way to handle them as an entire pellet, so I don't have to cfg. after each } fixation/dehydration step, and so they will be compacted for sectioning.
} Thanks in advance for any advice. } Marilyn Howton, Pathology Dept. West Virginia University
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From daemon Tue Dec 12 13:59:29 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Tue, 12 Dec 2000 14:56:35 -0500
Subject: Rotary shadowing

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Dear Listers,

Can anyone suggest a good reference or share a technique for preparing C-Pt
rotary shadowed collagen molecules on mica for TEM?

Thanks.

----------------
Ann Hein Lehman
EM Facility Manager
Trinity College
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu


From daemon Tue Dec 12 14:22:37 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Tue, 12 Dec 2000 08:42:07 -0000
Subject: Re: EM on cell cultures

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Dear Marilyn,

I am working with human cell lines in suspension and process the
cells in a pellet.

After the usual problems and much help from this list I worked out
a procedure which works for me.

It is critical that the pellet is small - in my experience not more
than 250 000 cells / pellet. This means you will not or hardly see
the pelleted cells until they have been in OsO4. All the dehydration
steps and infiltration steps are performed on a rotary shaker.

Here it is:

I transfer the cell suspension into conical BEEM capsules and spin
them in home made adapters at 900 x g. (It is essential to use a
swing bucket rotor, otherwise your cells smear along the sides of
the container and float off during the dehydration steps.)

After carefully removing the supernatant with fine tipped mini
pastetts I overlay the pellet with fixative, remove it again with
pastetts and so on. As soon as the OSO4 stains the pellet one
can see it and that makes the handling easier.
I use glass pipetts with OsO4 and propylenoxide and the resin-
propylen mixtures.

I usually infiltrate twice with epon-propylenoxide (1+2 and 1+1
respectively) for 45 minutes, then twice with pure resin. The tips of
glass Pasteur pipetts have to be cut to widen the opening
otherwise it is very difficult to remove the very viscous resin.

For sectioning I carefully flatten the very fine tip of the block with a
razor blade. The cells are evenly distributed, there is no need to cut
away surplus plastic. Just shape the block face to a trapezoid and
it can go straight into the ultramicrotome.

Good luck - if you require more details feel free to ask.

Regards
Claudia

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk





From daemon Tue Dec 12 14:58:03 2000



From: Owen P. Mills :      opmills-at-mtu.edu
Date: Tue, 12 Dec 2000 15:55:13 -0500
Subject: Jena microscope bulb

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Can anyone tell us where to buy a 12V 50W bulb part number HLWS5-A (by
Narva) for Jena scope. Please respond to me directly at the address below.

Thanks

Owen P. Mills
Electron Optics Facility Engineer
Michigan Technological University
Department of Materials Science and Engineering
Rm 512 M&M Building
Houghton, Mi 49931
Ph: 906-487-2002
FAX: 906-487-2934
Email: opmills-at-mtu.edu
URL: http://www.mm.mtu.edu/~opmills/index.html



From daemon Tue Dec 12 15:05:12 2000



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Tue, 12 Dec 2000 16:06:07 -0500
Subject: Summary

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Hi Everybody,

Here's a summary of the advice I received regarding my falling off section problem. It's long but read the last suggestion, it's my favorite.

Formvar coating the tradition way, or dipping the grid into formvar or 0.01% BSA. Dry by dragging grid across filter paper.
Bake the sections on to the grid-use either a 60 or 35 degree C oven overnight.
Heat fixing the sections using either a hot plate or slide warmer for 10 minutes.
Use Coat-Quick G.
Flame the grids with an alcohol lamp until the grid just changes color.
When working with Spurr's resin, use Veco grids not Gilder.
Make sure the water is utra-clean. Use double distilled, deionized, or microfiltered.
Various cleaning techniques:
1. 100% acetone wash.
2. 0.1M Nitric acid dip, rinse with dd water, dry on filter paper.
3. 50% acetic acid, 50% acetic acid, 100% acetone-dip grid into each solution then dry well before use.
4. 40 ml concentrated HCL in 400 ml dd water, add 40 ml acetone, take volume to 500 ml with dd water.
Sonicate grids in 50ml beaker for 1-2 minutes, discard solution and rinse 2 X with acetone (30 seconds each)
dry on filter paper. Do this every day to prevent oxidation from building up on the grids.
5. 100ml clean beaker, cover grids with 20ml glacial acetic acid, sonicate for 5-10 minutes, rinse with 100% acetone
until the smell of acetic acid is gone, rinse with 100% ethanol, pipette off the excess 100% ethanol, invert beaker
onto filter paper in a glass petri dish. Place in a 60 degree C oven for about 60 minutes, the grids will fall to the
bottom of the petri dish, cover the dish with the glass top, the grids will be good for a while. Glass is used to
avoid the static that plastic can have.
Put sections on the dull side.

Last but not least......

get a new job or get a technician.

I apologize for this being so lengthy, but I thought others might benefit from this information.



Paula :-)


Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax




From daemon Tue Dec 12 17:26:21 2000



From: francine.gauthier-at-mcc.gouv.qc.ca ()
Date: Tue, 12 Dec 2000 17:20:46 -0600
Subject: Ask-A-Microscopist: identify types of starch

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Colleagues...

Can anyone answer this question . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor

---------------------------------------------------------------------------

Email: francine.gauthier-at-mcc.gouv.qc.ca
Name: Francine Gauthier

Question: Is it possible to identify types of starch glue (rice, wheat,
corn, potato, etc)under microscope? Is there any visuals available
somewhere for comparisons?

---------------------------------------------------------------------------




From daemon Wed Dec 13 03:39:35 2000



From: GuessWho :      olivier.balmes-at-oorg2.lth.se
Date: Wed, 13 Dec 2000 10:32:32 +0100
Subject: TEM grid for plunging of organic solvents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear list members,

We are trying to do some cryo TEM of organic solvents based samples, and
the
grids we have used so far were not very satisfactory, some solvents
obviously
influenced the formvar (and maybe the carbon ?) of the grid making it
beam
sensitive.
We are planning on purchasing another type of grid (pure carbon lacey
film,
silicon dioxide or maybe silicon nitride) and I would like to know if
anybody
has tried anything for that kind of application.
Any suggestion or comment most welcome !

O.Balmes







From daemon Wed Dec 13 04:49:08 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 13 Dec 2000 11:41:46 +0100
Subject: S4700 and JSM 6700F (fwd)

Contents Retrieved from Microscopy Listserver Archives
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Dear colegues

Thanks to all how ansered my questions about these two FE-SEM. Its a pitty
that I heard about MSA Listserver only two weeks ago. It would have been
interesting to become these informations a bit sooner. It was very
fruitfull to have some return of experience and offline discussions with
some. Amazing was that amost only Hitachi owners had something to tell.
And they are all pleased about their instrument. True that the Jeol is
new, and much different from the former.

We have seen that our conclusions are similar that a lot of others, and so
in a few words, what we can say about these two SEMs:

The Jeol 6700F seems to have at low primery energy (1-2 keV) and
short working distance (1-3 mm) the best resolution and the easiest way to
become nice images. Charging effect are weak, and magnetic samples have
no visible propensity to "fly" in the chamber (magnetic nano powder, for
exemple) ! The limitation now, is that this good resolution is lost very
fast with increasing the working distance (6-8 mm). And the BSE working
distance is 6-8 mm, analytical working distance 15 mm. Shure that in BSE
or in X ray microanalysis, higher energy will bee used. But...and tilt ?
And the vacuum chamber is limited in avaible ports.

On the other hand, the Hitachi may have the capacity to do so
good, but it needs more experience of the manipulator and more time.
One is shure : the "In Lens" detector of the Hitachi works well (is
probably the best) at short and long working distances, gives a realy
surface information, and with the last improvement, gives the possibility
of composition contrast at low energy (schould be less sensitif to charge
effects too, but we didn't be convinced).

So, mesured with a ladle (do you say so in english ? "Mesurer à la
louche", in french !) The Jeol seems to be best for ultra high resolution
at low energy and short working distance. The Hitachi seems to be best if
you want to have the best images possible at analytical distance (12 mm),
backscatered detector on place and the two "secondary" dectectors
together. And is also capable to reach ultra high resolution. We didn't
make detailed tests at high energy (} 15 keV). All instruments seems to be
comparable at high energy, and then you don't observe the real surface.

But, and now I'll conclued, the two apparatus are VERY good, and it is
VERY difficult to make a choice. If you have monney, buy one of eatch ! We
have choiced the Jeol one, but whith some regree for Hitachi's "In Lens"
detector, versatility and reliability.

I've said nothing about Leo's 1500 serie and Philips XL30-S-FEG, witch are
also interresting. We have test them, but it would be to long to speak
about these now. But cold FEG seems to give better high resolutions
images,and is to be prefered if you don't need the long term stability of
Shottky FE, for intensive XRay microanalysis fot exemple. Gemini's optical
concept is very interresting, but more "classical" design give as good
results.

I'm still intersted on other opinions or test results, and also avaible
for questions, true MSA list server or on my direct mail.

By and thanks to all


J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From daemon Wed Dec 13 06:19:55 2000



From: olivier.balmes-at-oorg2.lth.se :      olivier.balmes-at-oorg2.lth.se
Date: Wed, 13 Dec 2000 13:12:01 +0100
Subject: TEM grids for plunging of organic solvents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear list members,

We are trying to do some cryo TEM of organic solvents based samples, and
the
grids we have used so far were not very satisfactory, some solvents
obviously
influenced the formvar (and maybe the carbon ?) of the grid making it
beam
sensitive.
We are planning on purchasing another type of grid (pure carbon lacey
film,
silicon dioxide or maybe silicon nitride) and I would like to know if
anybody
has tried anything for that kind of application.
Any suggestion or comment most welcome !

O.Balmes







From daemon Wed Dec 13 07:28:27 2000



From: DrJohnRuss-at-aol.com
Date: Wed, 13 Dec 2000 08:19:25 EST
Subject: Practical Stereology textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subject: J. C. Russ, R. T. Dehoff, Practical Stereology, 2nd Edition, Plenum
Press, New York, 2000 (!?!), isbn 0-306-46476-4

Some folks on this list have an early draft of my forthcoming (more on that
below!) book on stereology, which was distributed on the CD with the Image
Processing Tool Kit and also handed out in notes form at the various image
analysis workshops that I’ve taught for the past many years. Bob Dehoff (who
teaches those workshops with me) and I completed the final version of that
book in December, 1999, and sent it in to the publisher. It has taken them a
very long time to get around to doing anything with the book, and they are
still apparently having a lot of internal technical problems (not worth going
into here - but *.doc files and *.tif files on a CD seem to be a little too
high tech for these people, and they clearly don’t have any idea what a *.pdf
file is good for). At the M&M meeting in Philadelphia, quite a few people
asked me about the book and at that time Plenum was promising that it would
be available in September. Well, of course, it wasn’t, and still isn’t and
frankly I’m getting tired of apologizing for it. None of the delay has
anything to do with the authors, it is strictly production difficulties and a
high level of technical incompetence at the publisher’s end. Anyway, to make
a long story less long, I’ve put a copy of the entire book on the web in the
form of a pdf file. In order to keep the download times reasonable (it is
still 7.1 MB) the graphics are jpeg compressed, but I think still useful and
legible. Anyone who wants to download a copy is welcome to it. When the book
eventually (!) comes out the on-line version will evaporate. You may want to
purchase a copy to get the index and somewhat better graphics, but I will
leave that to your conscience. For the present, you can find a link to it at
{http://members.aol.com/ImagProcTK/updates} (look toward the bottom of the
page under "Data Analysis") or on my web page
{http://members.aol.com/DrJohnRuss} (go to the "links" section). In the
on-line file, the contents are linked to the book pages and of course you can
use Acrobat Reader to search for any word in the text.

John Russ


From daemon Wed Dec 13 07:54:22 2000



From: Walling, Dennis :      dwalling-at-utmb.edu
Date: Wed, 13 Dec 2000 07:48:39 -0600
Subject: Tissue staining question

Contents Retrieved from Microscopy Listserver Archives
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} I am looking for a tissue histology stain for human tongue
} epithelium for use with tetramethylrhodamine-labeled probes. The stain
} must not
} fluoresce under 546nm wavelength light and also must not quench
} tetramethylrhodamine fluorescence. Haematoxylin, eosin, and methylene
} blue each
} fail to meet these requirements. Does anyone have any suggestions?
}
} Dennis M. Walling, M.D.
} Assistant Professor
} Division of Infectious Diseases
} Department of Internal Medicine
} The University of Texas Medical Branch
} 301 University Boulevard
} Galveston, TX 77555-0835
}
} Telephone: (409) 747-2361
} Fax: (409) 772-6527
} E-mail: dwalling-at-utmb.edu
}




From daemon Wed Dec 13 08:18:26 2000



From: Rozeveld, Steve (SJ) :      SJROZEVELD-at-dow.com
Date: Wed, 13 Dec 2000 09:10:01 -0500
Subject: open position: Particle analyst- Light microscopy

Contents Retrieved from Microscopy Listserver Archives
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POSITION OPEN

The Materials Characterization Group in Dow’s Corporate R&D Analytical
Science laboratory has a full-time professional level opening for a particle
analyst/light microscopist at Dow’s facility in Midland, MI. The primary
responsibilities include working with partners to support research and
production projects across a broad range of products. Good written and oral
communication skills and the ability to work both independently and in a
team environment are extremely important.

Key responsibilities will include:
* Strong problem solving skills
* Operation of particle size characterization instrumentation
* Operation of light microscopes and supporting image analysis
software packages
* Method development
* Documentation of work
* Compliance with safety and quality programs

Qualifications:
* A candidate with a BS or MS degree in material science, chemistry,
or physics with experience in light microscopy and particle size
characterization.
* Experience with common commercially available particle size
measurement instrumentation - single-particle, ensemble and fractionation
based.
* Experience in the general area of polarized light microscopy,
digital image acquisition, and quantitative image analysis.

Please e-mail or send your resume and cover letter, with reference to this
ad to: Email: {R&D-at-Dow.com} or The Dow Chemical Company, Employee
Development Center, Workforce Planning, Midland, MI 48674. Email
respondents must list Job 00622LUSA/JMK-JB and their last name as the first
and second items on the subject line. Only those selected for an interview
will be contacted.

We are an equal opportunity employer and offer a competitive compensation
and benefits package including 401k, stock purchase, tuition reimbursement
and performance incentives. The Dow Chemical Company is the fifth largest
chemical company in the world with annual sales of US$20 billion. Dow
manufactures and supplies chemicals, plastics, and agricultural products for
customers in 164 countries and employs approximately 43,000 people
worldwide. For more news and information about Dow, visit our web site at
www.dow.com.



From daemon Wed Dec 13 08:18:26 2000



From: Rozeveld, Steve (SJ) :      SJROZEVELD-at-dow.com
Date: Wed, 13 Dec 2000 08:10:13 -0600
Subject: open position: Polymer Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Polymer Microscopy

Company: The Dow Chemical Company

Location: Midland, Michigan

Qualifications (education, certification, language, etc.) and experience
required:

A candidate with a MS or Ph.D. degree in polymer science, material science
or chemistry is preferred with prior experience in electron microscopy.
Good written and oral communication skills and the ability to work both
independently and in a team environment are extremely important.

Job Overview:

The Surface/Microscopy/X-ray (SMX) Group in Dow's Corporate R&D
Analytical Science Laboratory has a professional level full time opening for
a polymer microscopist. The primary responsibilities include working with
partners to support research projects involving new and existing products in
Dow's polymer businesses.

Key responsibilities will include:

1. Extensive problem solving.
2. Microscopy preparation technique experience including
3. Ultramicrotomy and cryo-ultramicrotomy.
4. Operation of light microscopes, scanning and transmission electron
microscopes.
5. Interpretation of images.
6. Documentation of work.
7. Compliance with safety and quality programs.
8. Active member of project and SMX work teams.

We are an equal opportunity employer and offer a competitive compensation
and benefits package including 401k, stock purchase, tuition reimbursement
and performance incentives.
Please e-mail or send your resume and cover letter, with reference to this
ad to:
Email: {R&D-at-Dow.com} or The Dow Chemical Company, Employee Development
Center,
Workforce Planning, Midland, MI 48674.
Email respondents must list Job 00623LUSA/JMK-JB and their last name as the
first and second items on the subject line.

Only those selected for an interview will be contacted.





From daemon Wed Dec 13 08:21:55 2000



From: SMancuso-at-specialmetals.com
Date: Wed, 13 Dec 2000 09:18:34 -0500
Subject: Remote SEM

Contents Retrieved from Microscopy Listserver Archives
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Can anyone tell me where I can find information on remote SEM?
Is it possible to install remote SEM capabilities on an older existing
instrument (JEOL 35C)?
Does this technology work for LOM also?


Sam O. Mancuso
Special Metals Corporation
Research & Development
New Hartford, NY 13413
smancuso-at-specialmetals.com




From daemon Wed Dec 13 09:03:29 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 13 Dec 2000 08:56:16 -0600
Subject: Ask-A-Microscopist: Starch grains

Contents Retrieved from Microscopy Listserver Archives
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Francine,

In my opinion, light microscopy is probably most useful in differentiating
starch grains, although SEM is sometimes used.

By using a light microscope with two polarizing filters, it is possible to
view a "cross" or "x-shaped" pattern within the starch grains. (This has
sometimes been referred to as a "Maltese Cross".) The appearance of this
cross can be an indicator of starch type. Also, by rotating one of the
polarizers you can observe the cross as it comes into view, rotates a bit,
then disappears again. Measuring the rotation arc of the polarizer within
which the cross is visible gives you another criterion.

Other ways of telling starches apart are, of course, their size and shape.
Sizes should be determined by averaging a number of grains, since they can
be quite variable---in fact, the amount of variation in size is yet another
criterion. Shapes range from round to various types of ovals and irregular
ovoids. SEM excels at this, allowing very precise measurements.

If you really want to get involved, it's possible to set up heated water
baths and sample a slurry of starch grains as the temperature rises, then
count the melted versus unmelted grains in the light microscope. Melted
starch grains are amoeba-like blobs, instead of nicely defined shapes.
Different starches have different melting characteristics.

There is a two-volume set of work by Edward Tyson Reichert (1913), named
"The Differentiation and Specificity of Starches in Relation to Genera,
Species, Etc." It is loaded with methods and photos.

Here is a web site you may want to look at:
http://www.siu.edu/~ebl/amylose.htm. I used to do starch research in the
lab that puts this page out, back in "the old days".

Hope this gets you started. Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Wed Dec 13 09:14:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Wed, 13 Dec 2000 09:09:25 -0600
Subject: Re: Remote SEM: TelePresence Tutorial at M&M 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} -----cut & paste-------- {
} Can anyone tell me where I can find information on remote SEM?
} Sam O. Mancuso

===================================================

You will need to carefully define what you mean by "Remote SEM".
Do you mean true TelePresence or just TeleObservation.

You should begin by looking at the Microscopy and Microanalysis
Proceedings for
1995, 1996, 1998, and 2000 there are a number of full session as well
as articles in there by the various groups in the world working in this area.

Viewing images on-line is do-able in virtually any imaging system, with
sufficient hardware upgrades, but remote "TeleOperation" can be a much
more involved proposition particuliarly for an older instruments.

You might consider getting a copy of the MSA Education Committee Video Tapes
made during the Tutorials presented at the Microscopy and Microanalysis 2000
meeting.

http://www.msa.microscopy.com/MSADocs/MSAVideotapeCatalogue95.html

I did a presentation therein called Microscopy and Microanalysis over the
Internet,
which discussed a pretty full range of options. I don't know if 2000
meeting videos
are ready yet, but you can contact Greg Erdos for that information at the
above
WWW page.


There are a number of vendors which can interface older instruments
to computer systems for image acquisition which the minimum starting point.
This can then, in principle, be adapted to TeleObservation. You should
talk to
the following companies: 4Pi, Quartz, Gatan , EmiSpec (to name a few
there are more
so please check the exhibitors list from the M&M 2000 meeting)
as well as literally any company that manufacturers EDS equipment (a long
list....).
Most of the Electron Optical manufacturers also offer or will soon offer
this as options on
their newer instruments but in all likelihood for your older instrument
you will have to go to the accessory market arena.

There is also going to be a session at the Microscopy & Microanalysis
Technologist's Forum at the 2001 Meeting which will discuss the
issue of TelePresence, you may wish to attend that meeting in Long Beach
August 5-9, 2001.
http://www.microscopy.com/MSAMeetings/MM01/MMHomePage.html

Nestor
Your Friendly Neighborhood SysOp

Disclaimer: I have no commerical interests in the companies mentioned
herein, but you might say I have a mild vested interest in "TelePresence" and
it's application to Research and Education ;-)

As always my TPM Lab is open to the world.... http://tpm.amc.anl.gov.
your welcome to visit anytime...






From daemon Wed Dec 13 11:12:29 2000



From: Thearith H. Ung :      tung-at-qdots.com
Date: Wed, 13 Dec 2000 09:04:31 -0800
Subject: TEM Course

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

Does anyone know of any good TEM courses? Thank you.

Regards,
Thearith


_________________________

Thearith Ung, Ph.D.
Quantum Dot Corporation
26136 Research Road
Hayward CA 94545
Tel: (510)-887-8775 (x4125)
Fax:(510)-783-9729
www.qdots.com



From daemon Wed Dec 13 12:44:15 2000



From: emxray-at-uwindsor.ca
Date: Wed, 13 Dec 2000 13:35:33 -0500
Subject: Simard Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


J. W. Robinson
Mechanical, Automotive, and Materials Engineering
University of Windsor
Windsor, Ontario
N9B 3P4

Phone : 519-253-4232, ext 2598

Could anyone send me any information on the L. M. Simard company,
maker of the tri-mag sputtering source? I need to order replacement parts
and the last phone number I have for them is no longer in service. Any
help will be appreciated.

Thank you.

John



From daemon Wed Dec 13 12:44:20 2000



From: DrJohnRuss-at-aol.com
Date: Wed, 13 Dec 2000 13:37:31 EST
Subject: wrong link for stereology book...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ooops - guess I need to type more carefully. The correct URL for downloading
the Practical Stereology textbook is
{http://members.aol.com/ImagProcTK/update.html} . The link there goes to a
grad student's computer (someone who had enough storage space that I could
use for a while), and from time to time that machine may go off line (when he
is doing some "real" work), so if you don't get through at first be patient
and try again in a few hours or the next day.

Sorry for the hassle

John Russ



From daemon Wed Dec 13 14:51:03 2000



From: Ping Li :      pli-at-is.dal.ca
Date: Wed, 13 Dec 2000 16:45:09 -0400
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We just installed a Tecnai 12. I am not sure what is a reasonable charge for
users to use the TEM. We are at university and a self-supported EM suite.
Could you please let me know your opinion or the user fee (hourly rate)
charged at your work place? especially at Canadian universities.

Thank you.
Ping



From daemon Wed Dec 13 15:08:46 2000



From: Larry Stoter :      larry-at-care4free.net
Date: Wed, 13 Dec 2000 20:17:06 +0000
Subject: Re: Remote SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

There is a freeware/shareware application WinVNC which basically
allows remote control over a network of any other PC and the
applications running on that PC. While not a perfect solution, it
works well running PC-driven SEMs, such as the JEOL 5500/5600/5900.
We have experimented with it in the JEOL UK Application Lab and,
considering it is 'free', are happy with it.

Addressing your specific problem, you would first have to convert
your JSM-35 to a PC driven SEM. I don't know about the US but in the
UK, there are several 3rd party companies offering such conversions.
If you don't get any information via the list, contact JEOL Inc.

Once such a conversion has been done, I see no reason why you
shouldn't control it over a LAN with WinVNC.

The same applies to any equipment driven by a PC - connect to a LAN
and use WinVNC. In fact, I'm told it will work with any platform
connected to the LAN - Unix, Mac, etc.

Regards,
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com


From daemon Wed Dec 13 15:23:14 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Wed, 13 Dec 2000 13:19:12 -0800 (PST)
Subject: Technovit 8100

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers

I have a collaborator who wants to do IHC on colon tissue. She just bought
some Technovit 8100 but doesn't know how to use it. I use Technovit
7100 for regular histology on plant tissue but I have no protocol
experience with 8100, IHC or animal tissue. Does anyone have any
suggestions or recommended protocols for doing IHC with Technovit 8100?
This is for light microscopy.

Thanks
Pauline Yu
splene-at-pw.usda.gov
USDA-ARS-WRRC
Microscopist Technician



From daemon Wed Dec 13 15:47:02 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Wed, 13 Dec 2000 13:42:25 -0800
Subject: need TEM film holders

Contents Retrieved from Microscopy Listserver Archives
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Hi everyone,

w'ere short about 3 plate holders for our Jeol 100cx TEM camera film (3&1/2
by 4"). At $25 apiece new, we were wondering if anyone had some spare?
Or if anyone knows which websites would have these sorts of things
second-hand?

cheers
Liz McKenzie

*******************************************************
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025

*******************************************************


From daemon Wed Dec 13 18:57:10 2000



From: Blanchette-Mackie, E. Joan Dr. (NIDDK) :      joanbm-at-bdg8.niddk.nih.gov
Date: Wed, 13 Dec 2000 18:46:59 -0600
Subject: Post Doctoral position at NIH

Contents Retrieved from Microscopy Listserver Archives
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Post Doctoral Position open in a multiple disciplinary laboratory at NIH
We use molecular biology, biochemistry and image analysis (confocal and
electron microscopy) techniques for studies on lipid transport in cells.

I am searching for someone who has training in electron microscopy to
investigate cholesterol mobilization in cells.

The position is ASAP,

The position is for a person not more than 4 years post PHD.

Those interested should send their CV's as inserts to the E-mail.

Thank You

--------------------------------------------------
Dr. Joan Blanchette-Mackie
Chief; Section of Lipid Cell Biology
NIDDK / NIH
Building 8, Room 427
8, Center Drive
Bethesda MD 20892 - 0850

Phone: (301) 496 2050
FAX: (301) 402 0723
E-mail: joanbm-at-bdg8.niddk.nih.gov




From daemon Wed Dec 13 18:57:15 2000



From: Broderick, Daniel :      dbroderick-at-masonwells.com
Date: Wed, 13 Dec 2000 18:50:36 -0600
Subject: Atom probe Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Hello, I am a venture capitalist located in Milwaukee WI and am in need of
some basic information on Atom Probe Microscopy because I am evaluating a
potential investment in a company that manufactures such a device. What is
it and how is it different than TEM and SEM. What are the applications it
is used for? Are there companies that sell them? What do they cost? Any
references you can direct me to will be appreciated. Thank you.

Daniel Broderick
Managing Director
Mason Wells Biomedical
770 N. Water St.
Milwaukee WI 53202
414 765 7830
fx 414 765 7850




From daemon Wed Dec 13 22:44:40 2000



From: Mark Blackford :      mgb-at-ansto.gov.au
Date: Thu, 14 Dec 2000 15:39:03 +1100
Subject: least squares fitting of EELS spectra

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I have EELS spectra from a material contain a mixture of Ce3+ and
Ce4+ and wish to determine the relative amounts of each oxidation
state. To do this I have collected spectra from a Ce3+ standard and
a Ce4+ standard and propose to least squares fit these to the
spectrum from my mixed state sample.

I presume this type of thing has been done before and there are
probably any number of ways to implement the fitting using Excel or a
KaleidaGraph or some purpose written software. I thought I'd ask if
anyone would like to make their solution available to me or even just
offer suggestions.

The ideal solution would be if the fitting could be done within EL/P
or even DigitalMicrograph.

I look forward to any replies. Cheers,

Mark Blackford
TEM Group
Materials Division, ANSTO
PMB 1,
Menai, N.S.W.
Australia
2234

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily
represent the official views of ANSTO from which this message was
conveyed.


From daemon Thu Dec 14 04:22:33 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Thu, 14 Dec 2000 20:58:32 +1000
Subject: RE: Rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
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The answer to this very much depends on what you are charging
for. To find an answer, you need to identify (a) the net costs you
wish to recover: e.g. capital costs (to be recovered over what
period?, at what rate of interest?), running costs, salaries of direct
(em technician) and indirect (clerical, admin.) support staff
including overheads, any charges for space occupancy and
services etc., less any subsidies (b) make a realistic estimate of
the maximum number of hours per year that a charge can be
recovered from. The answer is a/b. Simple, isn't it? Well, no. In
practise you will probably find that b is dependent on a/b since all
categories of users are sensitive to price, but you will have to
model this differently for different categories of users.

Our charges per hour for self-help access to a Philips CM120
Biotwin are as follows: Departmental £15, Other departments £21,
other publicly-funded research organisations £35, industrial £70.
Non-attenders incur full charge for the period booked. These
numbers were not exactly arrived at using rocket science. We
charge extra for all materials consumed (film, processing) and for
direct user-support from a technician. We do not include capital
costs recovery in our charges.

I would strongly advise imposing a special concessionary charge
on users who insist on tipping their specimens and retaining
circlips into the guts of the Compustage! An electronically-operated
trap door beneath the operator's seat is also an attractive option.



Chris




} From: "Ping Li" {pli-at-is.dal.ca}
To: {Microscopy-at-sparc5.microscopy.com}


I've noted no response, so I better offer something.
The technique was more commonly used to visualize stretched DNA fibers and you
may find more references using the Kleinschmitt technique about 30 years ago.

I have used fixed shadowcasting and negative staining on collagen too and both
methods "work". The advantage of rotary shadowcasting seems to be that it makes
it easier to follow the continuity of fibers (up-down, round and about). Rotary
shadowcasting is usually executed at a shallow angle of 6-12 degrees and so it
builds up more metal at the near vertical surfaces of the fibers.

Sticky-tape the very edge of the prepared specimen grid to a flat surface (half
of a microscope slide). A bit of tape may be required on two sides to assure
that the grid lies flat. Arrange the grid(s) a the rotation center of a rotator
within the vacuum chamber and locate the rotator to be between 60 and 100mm
from the evaporation source and a little lower than the source to achieve an
angle of say 10 degrees. The angle could be checked with a 10 degree card
segment.

The source could be C-Pt pellets or 0.1mm dia. Pt wire (try 40mm) wrapped
around a 1.5mm carbon cylinder about 5mm long that has been fashioned with a
suitable C rod sharpener. The "loaded" C rod is spring tensioned against a
second C rod that retains a flat end, but one that is reduced in size to about
3mm dia. using a conventional (but clean, e.g. no pencils) pencil sharpener.

Evacuate the system. Pt/C evaporation gives very fine granularity; about the
best short of special equipment, but a good vacuum ( { 5x10-5 torr) is
essential. Prior to evaporation start the rotator and adjust the speed to 30 to
60 rev/ minute. Increase the ampere control to achieve red heat, then back off
to give the vacuum a moment to recover. Then increase ampere to a white heat
and observe using a dark shield until the fashioned carbon tip has evaporated.
Hope this helps. This technique is much easier demonstrated than written about.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, December 13, 2000 5:57 AM, Lehman, Ann
[SMTP:Ann.Lehman-at-trincoll.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} Can anyone suggest a good reference or share a technique for preparing C-Pt
} rotary shadowed collagen molecules on mica for TEM?
}
} Thanks.
}
} ----------------
} Ann Hein Lehman
} EM Facility Manager
} Trinity College
} Hartford, CT 06106
} v. 860-297-4289
} f. 860-297-2538
} e. ann.lehman-at-trincoll.edu



From daemon Thu Dec 14 09:09:26 2000



From: Humphrey, Charles :      cdh1-at-cdc.gov
Date: Thu, 14 Dec 2000 10:04:22 -0500
Subject: unsubscribe

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Listserver,

unsubscribe.

Charles Humphrey


From daemon Thu Dec 14 10:28:03 2000



From: Sadhukhan, Pat :      SadhukhanPat-at-BFUSA.com
Date: Thu, 14 Dec 2000 10:24:30 -0600
Subject: Position: Material Scientist/Microscopist

Contents Retrieved from Microscopy Listserver Archives
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If you are a material scientist and have experience in microscopy and the
use of computers, then we invite you to apply for this position at the
Bridgestone/Firestone Research, Inc. in Akron, Ohio.

Our Microscopy Analysis Laboratories provide research and service in
microscopy for Bridgestone/Firestone Research, and, in general,
Bridgestone/Firestone (BFS) associates, as well as extend service to non-BFS
users. Currently, our laboratories have one 'state-of-the art' TEM/STEM/AIA,
one SEM/EDX, as well as one AFM/STM and a number of LOM-s and ancillary
equipments.

Duties of the appointee will include sample preparation, instrument
operation, analysis, and reporting. The level of appointment will depend on
the qualifications, and the experience of the successful candidate.

Please send a letter of application plus your CV (including the names,
postal-e-mail addresses, telephone/fax numbers of 3 referees) to:
Dr. Pat Sadhukhan
Bridgestone/Firestone Research, Inc. Phone: 330-379-7518
1200 Firestone Parkway Fax: 330-379-7530
Akron, OH 44317-0001


From daemon Thu Dec 14 10:34:33 2000



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Thu, 14 Dec 2000 10:31:47 -0600 (CST)
Subject: Damage to SrTiO3

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Has anyone come across radiation damage to SrTiO3, either
knockon or radiolytic?

-------------------------------------------------------
Laurence Marks
Director, Center for Transportation Nanotechnology
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Thu Dec 14 10:47:06 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 14 Dec 2000 08:44:02 -0800
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ping,
When we installed our TEM, we calculated the hourly cost of the service
contract, based on a 40 hour work week, and used that for a basis for our
charge to other users at the University. It is currently 50 dollars per
hour. The charge for commercial users is based on what commercial consultant
firms charge, in our area about 200 dollars an hour. There are, in my
experience, very few commercial users of TEM.
At 04:45 PM 12/13/00 -0400, you wrote:

} We just installed a Tecnai 12. I am not sure what is a reasonable charge for
} users to use the TEM. We are at university and a self-supported EM suite.
} Could you please let me know your opinion or the user fee (hourly rate)
} charged at your work place? especially at Canadian universities.
}
} Thank you.
} Ping
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Dec 14 11:25:35 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 14 Dec 2000 11:20:10 -0600
Subject: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

As a late follow-up to a somewhat recent string on liquid nitrogen safety, I
thought I would relate something that happened just this morning. While
transferring LN2 from one dewar to another using a funnel, the funnel
literally exploded just as the container was filled to the top. The
explosion was pretty forceful, sounding like a gunshot and scattering sharp
plastic shards all over the room and leaving behind a startled, but
otherwise unhurt technician (yup, it was me).

It was a standard-issue, large size plastic lab funnel, nothing special. We
had used the same funnel for over a year with no problems whatsoever. I
don't have a clue as to why it blew up or how there was enough pressure
generated to cause the explosion, but the remainder of the funnel looks a
lot like the hat Jughead used to wear in the Archie comics. It is now on
our bulletin board with a tastefully done joke notice about my recent brush
with eternity, courtesy of the other technician, Cheryl, who shall remain
anonymous.

Anyway, WATCH IT if you use the same low-tech LN2 transfer protocol we do
(or did).


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Dec 14 12:32:34 2000



From: Tom McKee :      tmckee-at-scilabs.com
Date: Thu, 14 Dec 2000 13:29:59 -0500
Subject: TEM-need PC based SAED indexing program users

Contents Retrieved from Microscopy Listserver Archives
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Good Morning MSA group

I am looking for comments from individuals who are currently using a PC
based program or spreadsheet to treat data from Al or Au ring SAED
calibration measurements. I would also like to be able to index minerals
such as asbestiform silicates. I am aware of older Fortran based
diffraction programs but I need a PC based system that someone is currently
using.

Thanks Tom McKee




From daemon Thu Dec 14 14:07:49 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Thu, 14 Dec 2000 15:02:29 -0500 (EST)
Subject: Re: wrong link for stereology book...

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On Wed, 13 Dec 2000 DrJohnRuss-at-aol.com-at-sparc5.microscopy.com wrote:

} Ooops - guess I need to type more carefully. The correct URL for downloading
} the Practical Stereology textbook is
} {http://members.aol.com/ImagProcTK/update.html} . The link there goes to a
} grad student's computer (someone who had enough storage space that I could
} use for a while), and from time to time that machine may go off line (when he
} is doing some "real" work), so if you don't get through at first be patient
} and try again in a few hours or the next day.

So far, today, none of the links get one to the download
site.

Kal




From daemon Thu Dec 14 14:10:00 2000



From: Zhenquan Liu :      zhenquan.liu-at-asu.edu
Date: Thu, 14 Dec 2000 13:07:40 -0700
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

The fee depends on the purpose of charging.

If you just want to pay the cost of maintenance for the users inside the
university, then I think that you just make a calculation based on the
costs on liquid N2, N2 gas, films, darkroom expenses, fees for service
contract, and the other consumption (everything related to the machine,
except the salary of the professional staff). Also the fee could be quite
different from university to university. Recently I have been two
universities, the same machine, JEOL 2000FX, one charges user USD$35 per
hour, the other charges user only USD$20. As I know, when you charge user
at high price although it is reasinable, then you will get less users to
use, finally you get less "income" to cover the cost. If it happens, you
may have to lower the price to encourage users to use. This happened to one
university I used to work.

Yes, people always charge those commercial companies at much higher price,
3 - 5 times?

Do a good calculation!

Zhenquan Liu














-------------------------------------------------------------

Dear Ping,
When we installed our TEM, we calculated the hourly cost of the service
contract, based on a 40 hour work week, and used that for a basis for our
charge to other users at the University. It is currently 50 dollars per
hour. The charge for commercial users is based on what commercial consultant
firms charge, in our area about 200 dollars an hour. There are, in my
experience, very few commercial users of TEM.
At 04:45 PM 12/13/00 -0400, you wrote:

} We just installed a Tecnai 12. I am not sure what is a reasonable charge for
} users to use the TEM. We are at university and a self-supported EM suite.
} Could you please let me know your opinion or the user fee (hourly rate)
} charged at your work place? especially at Canadian universities.
}
} Thank you.
} Ping
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca

------------------------------------
Zhenquan Liu (Dr.)
Arizona State University
CSSS, CHEM
Room PSA213,
Tempe, AZ 85287
Tel (480) 965 4544 (o)
(480) 775 7428 (h)
Fax (480) 965 9004 (o)
Email zhenquan.liu-at-asu.edu
------------------------------------




From daemon Thu Dec 14 14:10:03 2000



From: Tim Booth :      tbooth-at-em.agr.ca
Date: Thu, 14 Dec 2000 14:17:59 -0600
Subject: Position: Virology Lab EM technician job, Winnipeg

Contents Retrieved from Microscopy Listserver Archives
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Canadian Food Inspection Agency
National Centre for Foreign Animal Disease
Winnipeg

Salary: $ 45,146 - $ 54,928 per year
Tenure: indeterminate

Education: Preference may be given to candidates with a diploma or
degree in biology (or a related field) from a recognized
technical college or university.
Experience: Significant experience in a research/diagnostic laboratory,
including performance of electron and light microscopy techniques.
Experience in a variety of software applications, including digital
imaging
Experience in working with infectious agents.

Willingness to work under the rigours of high security biocontainment.
Contact with livestock outside of the workplace will be subject to
certain restrictions.

Functions:

To run a busy Reserach/Diagnostic EM lab, under supervision of a
Principal Investigator:

Prepares samples for diagnostic examination by electron microscopy;
operates electron microscope and conducts standard examination
procedures for detection, identification and characterization of foreign

animal disease agents by electron microscopy; establishes and confirms
primary identification of foreign animal disease agents in emergency
disease investigations and/or recruits the required expertise to
identify unknown agents; catalogues specimens, micrographs and images
and keeps accurate and comprehensive
records; develops new technical procedures or modifies existing
procedures for sample preparation and examination including observation
of frozen hydrated specimens by electron cryomicroscopy. Works on
immunohistochemistry techniques to label and localise antigens using
both light and electron mciroscopy.

If interested please email application including CV to Dr. Tim Booth,
tbooth-at-em.agr.ca
Candidates must clearly demonstrate in writing, that they fully meet the

screening criteria. Failure to do so will result in your application
being screened out of the selection process with no further
consideration being given to your application.

---



From daemon Thu Dec 14 14:12:58 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Thu, 14 Dec 2000 15:10:34 -0500
Subject: Re: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







As a late follow-up to a somewhat recent string on liquid nitrogen safety, I
thought I would relate something that happened just this morning. While
transferring LN2 from one dewar to another using a funnel, the funnel
literally exploded just as the container was filled to the top. The
explosion was pretty forceful, sounding like a gunshot and scattering sharp
plastic shards all over the room and leaving behind a startled, but
otherwise unhurt technician (yup, it was me).

It was a standard-issue, large size plastic lab funnel, nothing special. We
had used the same funnel for over a year with no problems whatsoever. I
don't have a clue as to why it blew up or how there was enough pressure
generated to cause the explosion, but the remainder of the funnel looks a
lot like the hat Jughead used to wear in the Archie comics. It is now on
our bulletin board with a tastefully done joke notice about my recent brush
with eternity, courtesy of the other technician, Cheryl, who shall remain
anonymous.

Anyway, WATCH IT if you use the same low-tech LN2 transfer protocol we do
(or did).


Dear Randy,
It is possible that internal stress was present in the funnel, and upon
cooling (this time) it built up past the ability of the funnel to adjust. This
happens in an altogether different temperature range to quarz high-pressure Hg
lamps. Teflon does not become too stiff at 77 K, so this shouldn't happen if
you use a teflon funnel.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Thu Dec 14 14:28:21 2000



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 14 Dec 2000 14:21:07 -0600
Subject: RE: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FWIW, I no longer use a funnel (would be S.Steel if I did). A number of
years ago I experienced the same scenario. My HDPE funnel exploded with
great force sending shrapnel ricocheting off the walls...

A bit of spilled nitrogen is not particularly hazardous (don't pour in your
shoe :) compared to the high speed funnel shards.

Woody White
McDermott Technology

-----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi Listers,
}
} As a late follow-up to a somewhat recent string on liquid
} nitrogen safety, I
} thought I would relate something that happened just this
} morning. While
} transferring LN2 from one dewar to another using a funnel, the funnel
} literally exploded just as the container was filled to the top. The
} explosion was pretty forceful, sounding like a gunshot and
} scattering sharp
} plastic shards all over the room and leaving behind a startled, but
} otherwise unhurt technician (yup, it was me).
}
} It was a standard-issue, large size plastic lab funnel,
} nothing special. We
} had used the same funnel for over a year with no problems
} whatsoever. I
} don't have a clue as to why it blew up or how there was
} enough pressure
} generated to cause the explosion, but the remainder of the
} funnel looks a
} lot like the hat Jughead used to wear in the Archie comics.
} It is now on
} our bulletin board with a tastefully done joke notice about
} my recent brush
} with eternity, courtesy of the other technician, Cheryl, who
} shall remain
} anonymous.
}
} Anyway, WATCH IT if you use the same low-tech LN2 transfer
} protocol we do
} (or did).
}
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}


From daemon Thu Dec 14 14:49:46 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Thu, 14 Dec 2000 15:46:06 -0500
Subject: S4700 and JSM 6700F (fwd)

Contents Retrieved from Microscopy Listserver Archives
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Hi

Just one point on your comments relating to charge in the Hitachi 4700.
In any SEM with an in lens detector the signal will be made up of SE.
Should you use the lower detector in the Hitachi you will find an increase
in the contribution of BSE and therefore a much lower charge rate.

Training many people in the use of the 4500 and 4700 I find they tend to
become upper detector addicts, when the lower detector often has much to
offer, particularly with samples that tend towards charging.

Hope this helps?

Steve Chapman

Senior Consultant Protrain
Tel & Fax - 44 (0)1280 814774
E-mail- protrain-at-emcourses.com
Web Site - www.emcourses.com
Protrain for SEM, TEM & EDX Training World Wide with our own full time
Professional staff


From daemon Thu Dec 14 15:08:37 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 14 Dec 2000 16:07:07 -0500
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
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At 4:45 PM -0400 12/13/00, Ping Li wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*********************************

Ping:
I run the EM Core facility for our medical college. I had to assure
my dean that my fees were in accord with other, similar facilities in
the NY-metro area. I called around to many of my colleagues and got
their fee schedules. My fees are about average...higher for some
things, lower for others. Briefly, our per hour fees for the EM are
$50 if the person has been trained and works alone, $75 if they need
my assistance. Film is billed at $1 each, which just barely covers
the cost...I'll probably have to raise that price this year.

If you'd like to see my whole price list (I have a fee for every
service available in the facility), you can go to to the website:
http://www.med.cornell.edu/research/cores/index.html
Go to the bottom of the page, click on the up-down arrows and scroll
to the Electron Microscopy Core.

I am responsible for covering all of the operating costs of the lab,
with the exception of my salary (thank heaven!). I am the sole
personnel in the facility for now, and handle about 100 clients/year.
I have received approval for a part-time tech, and will then offer
histology (paraffin embedding/sectioning, cryostat sectioning) as a
service rather than just having the equipment here for people to use
on their own.

Good luck. Setting up a fee schedule is no easy matter....be
prepared for loads of complaints about the cost, and people asking if
everyone pays the same rate. Everyone here pays the same fees, from
the Sr. Assoc. Dean of Research to the newest Assist. Prof. That way
no one gets their nose out of joint!

good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Dec 14 15:08:42 2000



From: Gerald Harrison :      jerry-at-biochem.dental.upenn.edu
Date: Thu, 14 Dec 2000 16:08:38 -0500
Subject: Hazards related to LN2

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Hello fellow Microscopy subscribers,

I think the point about hazards associated with LN2 that do not directly
involve the cold liquid itself is worthy of some note.

Over the 15 years we've been maintaining a LN2 cooled beryllium crystal,
there have been 3 incidents where luckily no injuries were sustained. Two
involved breaking the 4-L dewer -- once when a student smacked the top edge
of the dewer with the LN2 hose nozzle and once when a technician tripped
and fell with a full dewer. The first merely resulted in a very loud bang
with lots of fog, etc. as described in another post (no aluminum foil,
though) and very thankfully, the technician who fell was unhurt by either
the fall or the shattered glass. Again, lots of fog and running rivulets
of LN2 down the hallway.

The third incident was just a close call and one the was totally
unanticipated. Our dewer has a large cork in the top which is replaced
after the dewer is filled. After filling, the technician reached down to
pick up the nearly full dewer and, in the process, caused the LN2 to slosh
and a bit evaporated causing the cork to 'fire' out of the mouth of the
dewer (large 'pop gun' effect) and graze her face. She was not seriously
hurt beyond a small scrape on her temple...but, had she been hit in the eye
at such close range.... Anyway, since that graze incident, we who are
responsible for filling the EDAX dewer, wear glasses or goggles and are
very careful where the dewer is 'aimed' when we pick it up and carry it.

Gerald Harrison


From daemon Thu Dec 14 15:44:22 2000



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Thu, 14 Dec 2000 16:41:20 -0500
Subject: Re: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We have had similar occurrences here. Both old, and new funnels have
exploded. It is my guess that it is internal stress in the plastic which
causes
the event (Or, should I say the rapid release of the stress from being
cooled
to below minus 200 degrees centigrade?)

What we have done, is to use only stainless steel funnels for LN2.

Darrell



From daemon Thu Dec 14 17:20:57 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Thu, 14 Dec 2000 16:12:27 -0700
Subject: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
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I'd say the use of a plastic funnel with LN2 is a no no anyway. It probably
just shattered from being at that extreme temperature for a certain length
of time, which is why the "explosion" coincided with the other dewar being
topped off.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Thursday, December 14, 2000 10:20 AM
To: 'microscopy-at-sparc5.microscopy.com'


Hi Listers,

As a late follow-up to a somewhat recent string on liquid nitrogen safety, I
thought I would relate something that happened just this morning. While
transferring LN2 from one dewar to another using a funnel, the funnel
literally exploded just as the container was filled to the top. The
explosion was pretty forceful, sounding like a gunshot and scattering sharp
plastic shards all over the room and leaving behind a startled, but
otherwise unhurt technician (yup, it was me).

It was a standard-issue, large size plastic lab funnel, nothing special. We
had used the same funnel for over a year with no problems whatsoever. I
don't have a clue as to why it blew up or how there was enough pressure
generated to cause the explosion, but the remainder of the funnel looks a
lot like the hat Jughead used to wear in the Archie comics. It is now on
our bulletin board with a tastefully done joke notice about my recent brush
with eternity, courtesy of the other technician, Cheryl, who shall remain
anonymous.

Anyway, WATCH IT if you use the same low-tech LN2 transfer protocol we do
(or did).


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





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I'd say the use of a plastic funnel with LN2 is a no no anyway. It probably
just shattered from being at that extreme temperature for a certain length
of time, which is why the "explosion" coincided with the other dewar being
topped off.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Thursday, December 14, 2000 10:20 AM
To: 'microscopy-at-sparc5.microscopy.com'


Hi Listers,

As a late follow-up to a somewhat recent string on liquid nitrogen safety, I
thought I would relate something that happened just this morning. While
transferring LN2 from one dewar to another using a funnel, the funnel
literally exploded just as the container was filled to the top. The
explosion was pretty forceful, sounding like a gunshot and scattering sharp
plastic shards all over the room and leaving behind a startled, but
otherwise unhurt technician (yup, it was me).

It was a standard-issue, large size plastic lab funnel, nothing special. We
had used the same funnel for over a year with no problems whatsoever. I
don't have a clue as to why it blew up or how there was enough pressure
generated to cause the explosion, but the remainder of the funnel looks a
lot like the hat Jughead used to wear in the Archie comics. It is now on
our bulletin board with a tastefully done joke notice about my recent brush
with eternity, courtesy of the other technician, Cheryl, who shall remain
anonymous.

Anyway, WATCH IT if you use the same low-tech LN2 transfer protocol we do
(or did).


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Dec 14 19:11:49 2000



From: Gordon Nord :      gnord-at-mindspring.com
Date: Thu, 14 Dec 2000 19:53:17 -0500
Subject: Re: TEM-need PC based SAED indexing program users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

I am currently using Crystal Studio (check google to find the source) to
generate diffraction patterns for the amphiboles and others. Although it has
some strange characteristics the patterns are reasonable. Be advised that the
patterns are based on x-ray diffraction intensities - the actual SADs have
different intensity distributions as you might expect.

Email me if you need help indexing the asbestos silicates.

Tom McKee wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Good Morning MSA group
}
} I am looking for comments from individuals who are currently using a PC
} based program or spreadsheet to treat data from Al or Au ring SAED
} calibration measurements. I would also like to be able to index minerals
} such as asbestiform silicates. I am aware of older Fortran based
} diffraction programs but I need a PC based system that someone is currently
} using.
}
} Thanks Tom McKee

--
Gordon Nord
Small Business Network Design and Construction
Macintosh and Windows - Solutions and Conflicts

Nord Consultants
20594 Cornstalk Terrace
Ashburn VA 20147

Voice 703-723-2798 (Home Office)
Cell 703-403-2776 (Mobile Office)
Email gnord-at-mindspring.com




From daemon Thu Dec 14 21:14:01 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 14 Dec 2000 19:22:59 -0800
Subject: Fwd: RE: Rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Very good comment Jim

I would like just to point that it is not good idea "preheat" carbon rods
in the presence of sample. All contaminations from rods will contaminate
the sample first and than you will shadow that. Shadowing will develop not
only structural details but contamination ether. What I did, I preheat
rods without samples, than open the chamber, install the samples and pump
down the system again. Even when I done carbon film preparation, I prefer
to use the same technique. It takes more time. In general, shadowing
quality extremely depends from sample quality and sample preparation
procedure. Most air-dried protein samples demonstrate very poor structural
integrity preservation. Buffer composition is also important (you will
shadow not only the "sample" but salts from buffer as well).

Sergey


} Date: Thu, 14 Dec 2000 20:58:32 +1000
} From: Jim at ProSciTech {jim-at-proscitech.com}
} Subject: RE: Rotary shadowing
} To: "'Lehman, Ann'" {Ann.Lehman-at-trincoll.edu} ,
} "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com}
} Reply-to: "jim-at-proscitech.com" {jim-at-proscitech.com}
} Organization: ProSciTech
} X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Thu Dec 14 22:26:27 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Fri, 15 Dec 2000 10:15:41 +0200
Subject: FW: Remote SEM

Contents Retrieved from Microscopy Listserver Archives
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We have been doing some rotary shadowing to produce replicas on biological
samples for examination under the TEM. For shadowing we used V-shaped
tungsten wire and pure carbon for support,

They are a few problems we encountered. Every run is a trial and error
process. This is especially so with the setting of current to vaporize the
metal and the carbon. Every run is a different value although the voltage is
constant. At each run we try to follow the prefer parameter but somehow the
replica would look different from the previous one. Although, it is better
to observe the colour of the glow on the metal and carbon but that is very
hurting to the eye. I wonder if anybody has a definite value for the current
used to vaporize 0,1 mm pt wire/tungsten and pure carbon rod in the vacuum
chamber of 10 -5 torr.


Josephine
NUS

-----Original Message-----
} From: Jim at ProSciTech [mailto:jim-at-proscitech.com]
Sent: Thursday, December 14, 2000 6:59 PM
To: 'Lehman, Ann'; Microscopy-at-sparc5.microscopy.com



From daemon Fri Dec 15 03:49:00 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 15 Dec 2000 01:55:02 -0800
Subject: Fwd: FW: Rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
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Josephine
This message is only partially related to your topic, but, anyway. This is
my comment.

I would say, it's very unlikely to have good reproducibility in your
set-up. The easiest and probably cheapest solution is to use thickness
monitor. It's relatively cheap (prices vary from $1500 to $5000),
precisely measure the thickness starting from a few angstroms, easy to
work. You may control the shutter by the thickness monitor in order to
close the shutter at some point, say 2 nm.

More "solid" solution is to use electron guns for shadowing. I am using
electron guns for decade and they shown outstanding ability to evaporate
many substances: W, Pt/C, C in my case. Electron gun evaporated carbon
films have outstanding stability (in compare with thermal evaporated) under
the beam. I would mention that I forgot the time when I have a problem with
drift. Last time it was, probably 15 years ago when I was looking for good
technique for carbon film preparation. Additional reason to switch to the
Electron Gun - sample thermal damage. Electron guns produces less
infra-reds than regular set-up.

Another issue, I would like to comment is a level of vacuum in the
evaporators. The level 5x10-5 torr in my point of view is not acceptable
for ANY applications related to biology. The abequate range started from
2x10-6 torr I believe. I would point that practically all commercially
available evaporators are able to pump system down to the good 10-6 even
without LN2 trap. 10-5 is a result of bad evaporator's maintenance. Replace
diffusion oil on SANTAVAC 5 (and forget the problems with DP), check the
O--rings for cracks, replace old O-rings (do you know how old your
system?), keep system clean, run system at least for 24 h a month. I just
could not believe that films produced at 10-5 torr would not contaminated
by hydrocarbons from oil, from that a problem, many colleagues described:
bad hydrophilic properties of the films.

Sergey

} Date: Fri, 15 Dec 2000 12:20:19 +0800
} From: "Howe L. C. Josephine" {michowej-at-nus.edu.sg}
} Subject: FW: Rotary shadowing
} To: "'microscopy-at-sparc5.microscopy.com'" {microscopy-at-sparc5.microscopy.com}
} Cc: "'jim-at-proscitech.com'" {jim-at-proscitech.com}
} X-Mailer: Internet Mail Service (5.5.2650.21)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Dec 15 05:58:13 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Fri, 15 Dec 2000 21:52:22 +1000
Subject: RE: Rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pt coating is not as fine as simultaneous Pt - C. Support film (carbon backing)
is only needed if you need to dissolve a thick biological specimen; for thin
fibres (say unstained biological material under 50nm) a replica is rather
cumbersome.
I expect that you also have problems with Pt forming an amalgam with W and so
varying amounts are evaporated. That is another advantage of using Pt - C.
Another problem is that for C evaporation you do best at around 30 Volts and
for metal, you achieve much better control with about 10 volts. The volt
settings on some evaporators can be changed but the variable that you are using
is changing amperes not volts. I expect that the control of one of your
electrodes is very touchy.
So take a pick, you have several problems and you may be able to eliminate one
or two.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, December 15, 2000 2:20 PM, Howe L. C. Josephine
[SMTP:michowej-at-nus.edu.sg] wrote:
} We have been doing some rotary shadowing to produce replicas on biological
} samples for examination under the TEM. For shadowing we used V-shaped
} tungsten wire and pure carbon for support,
}
} They are a few problems we encountered. Every run is a trial and error
} process. This is especially so with the setting of current to vaporize the
} metal and the carbon. Every run is a different value although the voltage is
} constant. At each run we try to follow the prefer parameter but somehow the
} replica would look different from the previous one. Although, it is better
} to observe the colour of the glow on the metal and carbon but that is very
} hurting to the eye. I wonder if anybody has a definite value for the current
} used to vaporize 0,1 mm pt wire/tungsten and pure carbon rod in the vacuum
} chamber of 10 -5 torr.
}
}
} Josephine
} NUS
}
} -----Original Message-----
} From: Jim at ProSciTech [mailto:jim-at-proscitech.com]
} Sent: Thursday, December 14, 2000 6:59 PM
} To: 'Lehman, Ann'; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Rotary shadowing
}
} ------------------------------------------------------------------------
} The Microscopy ListServer-Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe-Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I've noted no response, so I better offer something. The technique was more
} commonly used to visualize stretched DNA fibers and you may find more
} references using the Kleinschmitt technique about 30 years ago.
} I have used fixed shadowcasting and negative staining on collagen too and
} both methods "work". The advantage of rotary shadowcasting seems to be that
} it makes it easier to follow the continuity of fibers (up-down, round and
} about). Rotary shadowcasting is usually executed at a shallow angle of 6-12
} degrees and so it builds up more metal at the near vertical surfaces of the
} fibers.
} Sticky-tape the very edge of the prepared specimen grid to a flat surface
} (half of a microscope slide). A bit of tape may be required on two sides to
} assure that the grid lies flat. Arrange the grid(s) a the rotation center of
} a rotator within the vacuum chamber and locate the rotator to be between 60
} and 100mm from the evaporation source and a little lower than the source to
} achieve an angle of say 10 degrees. The angle could be checked with a 10
} degree card segment.
} The source could be C-Pt pellets or 0.1mm dia. Pt wire (try 40mm) wrapped
} around a 1.5mm carbon cylinder about 5mm long that has been fashioned with a
} suitable C rod sharpener. The "loaded" C rod is spring tensioned against a
} second C rod that retains a flat end, but one that is reduced in size to
} about 3mm dia. using a conventional (but clean, e.g. no pencils) pencil
} sharpener.
} Evacuate the system. Pt/C evaporation gives very fine granularity; about the
} best short of special equipment, but a good vacuum ( { 5x10-5 torr) is
} essential. Prior to evaporation start the rotator and adjust the speed to 30
} to 60 rev/ minute. Increase the ampere control to achieve red heat, then
} back off to give the vacuum a moment to recover. Then increase ampere to a
} white heat and observe using a dark shield until the fashioned carbon tip
} has evaporated. Hope this helps. This technique is much easier demonstrated
} than written about.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, December 13, 2000 5:57 AM, Lehman, Ann
} [SMTP:Ann.Lehman-at-trincoll.edu] wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer-Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe-Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listers,
} }
} } Can anyone suggest a good reference or share a technique for preparing
} C-Pt
} } rotary shadowed collagen molecules on mica for TEM?
} }
} } Thanks.
} }
} } ----------------
} } Ann Hein Lehman
} } EM Facility Manager
} } Trinity College
} } Hartford, CT 06106
} } v. 860-297-4289
} } f. 860-297-2538
} } e. ann.lehman-at-trincoll.edu



From daemon Fri Dec 15 07:05:43 2000



From: DrJohnRuss-at-aol.com
Date: Fri, 15 Dec 2000 07:59:44 EST
Subject: Problems downloading stereology book?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK Folks, I am open to suggestions. The computer with the file on it is still
down, and I'm getting tired of all the nagging emails. Who can help me? The
file is 7.5 MB. Who has enough space and reasonable access and is willing to
host the file? The only conditions are that when the printed version of the
book eventually appears, the electronic on-line version will need to be
removed. In the meantime, anyone who wants to download the pdf file is
welcome. If you are interested in providing host space, please contact me
(off the list, please). Also note that there has to be some way for me to get
the file to you - many mail systems won't accept such a large attachment.

John Russ
John_Russ-at-NCSU.edu or DrJohnRuss-at-AOL.com


From daemon Fri Dec 15 07:37:01 2000



From: William.SHort-at-cpe.amedd.army.mil
Date: Fri, 15 Dec 2000 07:31:41 -0600
Subject: Ask-A-Microscopist: Verein Deutscher Ingenieur 3492 Method for

Contents Retrieved from Microscopy Listserver Archives
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Email: William.SHort-at-cpe.amedd.army.mil
Name: William Short

Question: One of my tasks is to implement the Verein Deutscher Ingenieur
3492 Method for analyzing asbestos fibers and other indoor pollutants
using the Scanning Electron Microscope and an Energy
Dispersive X-ray Analyzer.
At this point I am nearly completed with the implementation.
We have a nice old Hitachi S-450 model SEM and we use the KEVEX
EDX to get our spectral data off the unknown samples. I am putting
together the paperwork and reports to tell our customers what it is
that we analyzed from their sample submissions. I give a Concentration
result, the instrument Sensitivity determined by the filter area and air
volumes, the raw data, fiber types ( i.e. asbestos, inorganic, calcium
sulfate, or other), and a 95% Confidence Interval using the Poisson
distribution. Also included are the fiber dimensions, voltages,
a picture of the fiber morphology, scale in nanometers, and a copy
of a spectral spot return from the EDXA.
My problem is that I don't know if I'm producing the correct
results for the Confidence Interval calculation using the Poisson function.
Aren't there special considerations for the results obtained from getting 0
fibers,
1 fiber, 2, fibers, and 3 fibers ? Can you help me with this area or will
I just have to "punt" ? The VDI 3492 Method is being used in Europe for this
analysis to comply with the Federal Governing Standards for Host nations.

Any information that you could give me would be greatly

---------------------------------------------------------------------------




From daemon Fri Dec 15 08:21:07 2000



From: Hyman, S.C. :      sch10-at-leicester.ac.uk
Date: Fri, 15 Dec 2000 14:14:37 -0000
Subject: Metal shadowing

Contents Retrieved from Microscopy Listserver Archives
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On the subject of metal shadowing, might I recommend the excellent treatise
by Willison and Rowe (my ex-head of department!)
"Practical Methods in Electron Microscopy - Volume 8" - One of the
excellent Glauert edited series.


From daemon Fri Dec 15 08:38:06 2000



From: Earl Weltmer :      eweltmer-at-home.com
Date: Fri, 15 Dec 2000 06:35:32 -0800
Subject: EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I have some used EDS detectors from various SEMs that I would like to give
free to anyone who has use for them & pay the shipping costs.

They were working then stored ,uncooled for years so they are probably not
working now but might be used as a trade-in for a new system or ?

Please contact me offline if interested.

Thank You,


Earl Weltmer
(714) 573-9158



From daemon Fri Dec 15 09:27:35 2000



From: Heidi Smith :      SMITHH-at-em.agr.ca
Date: Fri, 15 Dec 2000 10:21:27 -0500
Subject: TEM film distributers

Contents Retrieved from Microscopy Listserver Archives
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Seasons greetings to all!

I am currently in the process of ordering Kodak electron microscope film 4489 (3.25 x 4 in.), however, it seems that I can no longer order the film from Kodak Canada Inc. Kodak is still producing the film but only in the U.S. I guess. This may not seem problematic but the price of the film from the Canadian distributers is at least two times as much (almost three times in one case) as the price paid the last time it was ordered.

I believe that AGFA produces a similar type of film but I do not know the product name or who to contact. If anyone has information on distributers, of either Kodak or the AGFA film, with reasonable prices I would appreciate hearing from you. Alternatively, if anyone has another favorite TEM film that they use and would like to share that information I would be happy to hear from you.

Please send me the information offline at the following address:
smithh-at-em.agr.ca

Thank you in advance!
Heidi Smith
Microscopy Technician
Agriculture and Agri-Food Canada
Kentville, NS, Canada



From daemon Fri Dec 15 10:17:37 2000



From: Maria Ericsson :      maria_ericsson-at-hms.harvard.edu
Date: Fri, 15 Dec 2000 11:11:22 -0500
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
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} Dear Ping,

I've been running the Core EM Facility at Harvard Medical School in Boston
for about 5 years now. When I started the lab, I had absolutely no clue
what to charge, so I checked out what other Facilities were charging. After
a little research (but still feeling pretty clueless) I started charging
30$/ beam hour for Scope time, which I think was was below average in 1995.
There weren't that many EM labs in the area so I took an average of whoever
I got hold of across the country..

My idea was to charge a little less to get people interested. Which I think
worked well (I had good financial support for a few years, until things
took off.. I don't know if your lab there is new or already
established..) Now that people are hooked, we have an average use of 50-60
hour/month on 2 scopes. I'm charging 31.50$/hour for Med. School users and
45$/hour for outside, for assisted Scope use I charge 63$/hour for Med.
School and 94$/hour for outsiders. With our current use, which has been
pretty stable for the last year, the income from scope use covers the
service contracts and supplies..

I think my rates are lower than average, but I have a huge user group. I
have two half time technicians and the Facility income pretty much covers
the operating costs now (..not including my salary..!)

I have a full fee-for serivce pricelist at
http://www.hms.harvard.edu/core/em.html

Good luck with the EM lab!

Maria


} At 4:45 PM -0400 12/13/00, Ping Li wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

____________________________

Maria Ericsson
Harvard Medical School EM Facility
220 Longwood Avenue
Boston, MA 02115
(617) 432 1698
maria_ericsson-at-hms.harvard.edu
http://www.hms.harvard.edu/core/em.html


From daemon Fri Dec 15 10:51:31 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Fri, 15 Dec 2000 11:44:21 -0500
Subject: call for abstracts

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Call For Abstracts

Florida Society for Microscopy-Florida Chapter of the
American Vacuum Society & Surface Analysis 2001


Annual Symposium

Technical Meeting March 12-14 - Short Courses March 12-15
University of Central Florida; Student Union Building; Orlando, Florida



Meeting Highlights
* Technical Sessions: Microscopy in the Physical and Biological
Sciences, Surface Science, Thin Films, Focused Ion Beam Techniques,
Biomaterial Interfaces, Environmental Science, Metrology, and Electronic
Materials
* AVS & Vendor Sponsored Short Courses
* Job Fair
* 3rd Annual FIB Users Workshop, and Vacuum Educators Workshop
* Student Poster Competition (over $5000 in prizes can be awarded)
-plus the MSA Traveling Poster Display
* Equipment Exhibit with Surface, Analysis, Vacuum, and
MicroscopySciences Vendors
* No registration fee for Symposium or Equipment Exhibit

Confirmed Invited Speakers to Date
* Donald Baer Pacific Northwest National Lab
* Alice Dohnalkova Pacific Northwest National Lab
* Klaus Edinger University of Maryland
* Robert Hull University of Virginia
* Bill Lamberti Exxon
* Eero Ristolainen Tampere University of Technology
* Bruno Schueler Physical Electronics
* Catherine Taylor Wright State University
* Edgar Voelkl Oak Ridge National Lab



Abstract Submission
The abstract deadline is January 5, 2001 for hard copy abstracts and
January 12, 2001 for e-mail abstracts.
E-mail submissions are strongly encouraged. Contributed papers will be
presented as platform presentations
or poster presentations. Authors should indicate their session preference
and preferred presentation format
i.e., poster or platform. For uniformity, all the abstracts must be in the
following format: the title of the talk
should be in all capital letters followed by the names and addresses of the
authors. The presenter's name
should be underlined. Abstracts are limited to 200 words. E-mail
abstracts should be as text in the body of
the e-mail or an attached Microsoft Word file. Send abstracts to Dr.
Lucille Giannuzzi, Program Chair,
407 275-4354, lag-at-mail.ucf.edu. Authors will be contacted by their session
chair in mid-January 2001
concerning their abstract's status.


Registration
You may register on line by clicking on the following link:

http://natasha.eng.usf.edu/gilbert/avs/anouncement/registration.html


General Meeting Information
You may find more information by following
the "AVS Info; Chapters; Florida Chapter" links at:
www.vacuum.org or the "Local Affiliate
Societies" link at www.microscopy.org or contact Fred Stevie,
407-371-7626, stevie-at-lucent.com or Jo Ann
Moore, jamoore-at-com1.med.usf.edu 1-813-974-9446

In order to receive email updates and future
information about the meeting please forward your email address
to Pete Ries pjries-at-lucent.com.

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Fri Dec 15 11:01:49 2000



From: stemmer-at-rice.edu
Date: Fri, 15 Dec 2000 10:46:29 -0600 (CST)
Subject: Postdoctoral Position in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Position in Transmission Electron Microscopy - Rice University

Rice University, Department of Mechanical Engineering and Materials Science,
located in Houston, TX, is seeking candidates for a postdoctoral position in
transmission electron microscopy of multicomponent oxide thin films.
Applicants should have extensive and demonstrated experience in several areas
of TEM and a strong background and interest in materials problem solving.
Preference will be given to candidates with experience in high-resolution
imaging and electron energy-loss spectroscopy as well as conventional
diffraction contrast imaging.
Facilities at Rice include a JEOL 2010, field-emission SEM and high-resolution
X-ray diffractometers, as well as state-of-the-art sample preparation. The
project will be carried out in close collaboration with the University of
Houston using a state-of-the-art field-emission TEM (JEOL 2010F), with annular
dark-field detector, Oxford link EDS, Gatan GIF and STEM capabilities. Ability
and interest to take part in an upgrade of existing facilities to high
resolution scanning TEM capability is expected. The position is available
immediately. Duration about 1-2 years, salary is commensurate with
qualifications. Candidates with a Ph.D. in Materials Science or Physics will
be given preferred consideration.
Interested candidates should send a curriculum vitae, publication list and the
names of at least three references with their contact addresses to:

Prof. Susanne Stemmer
Rice University
Department of Mechanical Engineering and Materials Science
MS 321
6100 Main Street
Houston, TX 77005-1892
stemmer-at-rice.edu

Applicants must have proof of legal authorization to work in the United
States.
Rice University is an Affirmative Action/Equal Opportunity Employer.



From daemon Fri Dec 15 11:54:26 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 15 Dec 2000 17:57:51 +0000 (GMT Standard Time)
Subject: Re: TEM film distributers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Heidi,

Unfortunately Agfa have recently stopped supplying their
Scienta film that we used in some of our TEMs so we are now
changing to Kodak 4489 or SO163 as the Agfa runs out.

Ron

On Fri, 15 Dec 2000 10:21:27 -0500 Heidi Smith
{SMITHH-at-em.agr.ca} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Seasons greetings to all!
}
} I am currently in the process of ordering Kodak electron microscope film 4489 (3.25 x 4 in.), however, it seems that I can no longer order the film from Kodak Canada Inc. Kodak is still producing the film but only in the U.S. I guess. This may not seem problematic but the price of the film from the Canadian distributers is at least two times as much (almost three times in one case) as the price paid the last time it was ordered.
}
} I believe that AGFA produces a similar type of film but I do not know the product name or who to contact. If anyone has information on distributers, of either Kodak or the AGFA film, with reasonable prices I would appreciate hearing from you. Alternatively, if anyone has another favorite TEM film that they use and would like to share that information I would be happy to hear from you.
}
} Please send me the information offline at the following address:
} smithh-at-em.agr.ca
}
} Thank you in advance!
} Heidi Smith
} Microscopy Technician
} Agriculture and Agri-Food Canada
} Kentville, NS, Canada
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri Dec 15 11:58:16 2000



From: DrJohnRuss-at-aol.com
Date: Fri, 15 Dec 2000 12:55:19 EST
Subject: Stereology book - problem fixed!!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are now several copies of the book available on different servers, so
access should not be a problem. The book is available at:

{http://loner.phys.lsu.edu/Users/brent/Stereology.pdf}
(Thanks to Brent Neal at Louisiana State Univ. in Baton Rouge. This was the
original host machine, but there was a general power failure just as I was
posting the original note - another proof of Murphy's Law - and it took a
while to get things back up; it is fine now)

{http://www.abdn.ac.uk/~ort056/stereology.pdf}
(Thanks to Jenny Gregory {j.gregory-at-abdn.ac.uk} in Aberdeen, Scotland)

{http://128.101.243.155/Stereology.pdf}
(Thanks to Mike Herron {herro001-at-umn.edu} at the University of Minnesota)

{http://www.biotech.ufl.edu/~emcl/Stereology.pdf}
(Thanks to Greg Erdos {gwe-at-biotech.ufl.edu} at the University of Florida in
Gainesville)

It should be available very soon from
{http://www.practical-stereology.org}
(Thanks to Greg Strout {gstrout-at-ou.edu} at the University of Oklahoma)

A few more sites are in process, and everything will be linked from the
original main pages at
{http://members.aol.com/drjohnruss/links.htm}
{http://members.aol.com/ImagProcTK/update.htm}

Many thanks to all of the others out there who offered help or advice.

John Russ


From daemon Fri Dec 15 14:28:17 2000



From: Shea Miller :      millers-at-em.agr.ca
Date: Fri, 15 Dec 2000 15:27:30 -0500
Subject: cryprotectants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone;
just before everyone disappears for the weekend.... I will be cutting some cryosections of infected wheat heads (on monday, of course), and wanted to fix briefly, then put into a cryoprotectant to keep the cells from rupturing when I section (for light microscopy). I know I have successfully used sucrose in the past, but I can't seem to find the concentration. (4% rings a very distant bell...). Can anyone out there tell me what works for them? (or if you have other cryoprotectants that you put your plant samples into before sectioning, I'd be interested in those, too!)

thanks, as always, in advance
shea



Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
E-mail: millers-at-em.agr.ca



From daemon Fri Dec 15 15:28:33 2000



From: George Langford :      amenex-at-amenex.com
Date: Fri, 15 Dec 2000 16:29:34 -0500
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists !

All this talk about what y'all charge your users is no different
than what prompted Congress to pass the Sherman Anti-Trust Act:

to protect Interstate commerce from price-fixing.

The discussion and setting of rates by communication
between vendors isn't proper to my legally untrained eye.

Though it's OK to publish your rates, if that's what
you really charge.

Now, it also turns out that what you charge users is nowhere
near what Amenex Associates as a private, for-profit company
has to charge to stay in business:

http://207.103.140.16/webpage/amnxfees.htm

Chuck Garber might want to chime in as to why independent lab
owners get steamed up about all this.

Best regards,
George Langford
amenex-at-amenex.com


From daemon Fri Dec 15 16:15:20 2000



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Fri, 15 Dec 2000 15:12:47 -0700
Subject: Request: EM images of veterinary specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To anyone who can help:

I was contacted today by a person who needs to locate some EM images of
veterinary specimens. They have an immediate need for images, probably
SEM's, of organs of the digestive system.

For further information, please contact them directly, not the listserv, at
the following:

Jill Kahn
952-852-7358
kahn-at-collemcvoy.com


I'm just the messenger,

John
john.chandler-at-colostate.edu
Colorado State University




From daemon Fri Dec 15 19:26:32 2000



From: Earl Weltmer :      eweltmer-at-home.com
Date: Fri, 15 Dec 2000 17:16:14 -0800
Subject: EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Thank you to evryone who responded to the free EDS detector.
The two that I had are now on their way to the University of North Carolina.

I still have one EDS detector that was removed working from a PHI Auger
microprobe.
I also have some line voltage stabilizers that were removed from a JEOL 840
SEM.
Keep in mind the stabilizers are HEAVY and the shipping costs are
proportional.

Please contact me offline for details.

Thank You,

Earl Weltmer
Scanservice Corp.
SEM Maintenance
(714) 573-9158





From daemon Fri Dec 15 19:49:08 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 15 Dec 2000 17:44:14 -0800
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can see your point of concern regarding this issue. However,
if I understand the context within these folks are discussing
fees, there is a different reference basis. They are talking about
user fees in an academic environment. These fees are for
services to members of their academic community--and in
fact, for members of their respective institutions. This is quite
different from for-profit enterprises' sphere of interest.

If the universities engage in external fee-based work,
your point would tend to have merit. But what these folks
are trying to do is to spread their cost center's burden
over those who are using it, within their small community
of users.

If the situation is other than this, of course, that is a
different issue.

gary g.


At 01:29 PM 12/15/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Dec 15 19:59:15 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 15 Dec 2000 17:56:49 -0800 (Pacific Standard Time)
Subject: rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
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I'm somewhat amazed by how many varied responses there are
on rotary shadowing technique, and how few seem to agree
with what works for us. So, here is yet another response.

We shadow biological molecules from the connective tissue
matrix, usually ranging in size from 16 to 300 kd. Many
are linear, some are globular. We spray the molecules in
solution with 70% glycerol. The other 30% is 100
microgram/ml of protein, preferably in a volatile buffer
such as 1% acetic acid or 0.1M ammonium bicarbonate, pH
7.8. Other buffers can be used but salt crystals can be a
big problem. Spraying is accomplished using an air brush,
and we spray onto freshly cleaved mica discs cut from
sheets using a hole punch.

The sample is dried in vacuum, though we often accumulate
20 samples so some drying occurs outside vacuum. We are
careful to pre-pump the vacuum chamber and heat the guns
thoroughly to out-gas, then vent the jar with nitrogen,
introduce the samples, and pump again. Our system uses a
turbo-molecular pump. We can vary the angle of the rotary
stage from outside the chamber, so we tilt the specimen
away from the gun and outgas thoroughly again so that little
vacuum loss is seen during evaporation. This is really
important. Gas is introduced to the rods whenever the
system reaches atmospheric pressure. It is also
important to keep the system clean, as outgassing a dirty
gun takes much longer than outgassing a clean gun. We try
to complete evaporation without entering the 10 -5 range,
and we begin in the 10-7 range. We evaporate at a slow
rate, usually taking 3 to 5 minutes to complete a run,
which also seems to improve resolution. Final film
resolution is very proportional to vacuum, the better the
vacuum the smaller the grain size. We use a quartz monitor
for controlling the amount of Pt-C coming from the electron
beam gun, but we also use a folded piece of filter paper
placed 90 degrees relative to the source, and monitor the
color which should be dark gray (not black, not brown). We
evaporate at 6 degrees relative to sample as the sample
rotates. Following Pt-C deposition, we then tilt the
sample to 90 degrees relative to a resistance carbon source
and evaporate a backing film of carbon onto the mica. The
thickness of the film is monitored with a piece of folded
filter paper placed 90 degrees relative to the carbon
source, and the correct amount is just visible tan color
(not gray) on the filter paper. Our film thickness
monitor is not sensitive enough to monitor carbon
deposition. We find this carbon film absolutely necessary
for sample stability, perhaps because we use so little
PT-C. However, too much carbon will certainly affect final
resolution, loosing edge detail. Finally, the replicas are
exposed to the vapors of 1% acetic acid in a petri dish for
about a half-hour prior to floating in distilled water (the
acid is very useful in helping the replica to release from
the mica (so that they float off as one intact film ). We
use high-transmission 600 mesh grids to support the films.

For many years we evaporated Pt wire from carbon rods using
a resistance source. We wound 2.3 cm of wire around a
cylindrical jig which was the same diameter as the
sharpened carbon rods (about 1mm). Prior to coiling the
wire, we passed it through a alcohol burner flame till it
was orange, which made it more malleable and perhaps cleaned
it a bit. The coil was transferred to the resistance
source, spanning the intersection of two rods
(therefore the site of most resistance and primary
heating) held together with moderate spring tension. The
carbon rods with accompanying Pt coil was at 6 degrees and
about 11 cm away from the mica discs. Using a welders
plate (Fullam #12511), we observed the metal as current was
increased through the rods and just after the wire melted,
the current was turned up just a bit more and left there
until the Pt was seen to evaporate completely. It was
necessary to observe this through the welders plate to get
a good shadow and know when to turn the current down to
avoid too much carbon. We evaporated a backing film of
pure carbon from a second source oriented at 90 degrees
from a distance of 11 cm so that the rods just started to
spark, counted to 1.5 seconds, at which time the amount of
carbon was probably about right (as judged by filter paper
color and a bit of luck).

Sorry for the novel,

Doug

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Sat Dec 16 04:23:29 2000



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Sat, 16 Dec 2000 19:17:04 +0900
Subject: Re: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Stainless steel funnel poses also some danger - taking it with bare hand
after the fill (no such problem with the plastic ones). The funnel I use has
a crack which makes it more safe from an internal stress point of view.

Rado



From daemon Sat Dec 16 10:42:35 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 16 Dec 2000 11:38:41 -0500
Subject: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gary Gaugler wrote:
==========================================================
I can see your point of concern regarding this issue. However, if I
understand the context within these folks are discussing fees, there is a
different reference basis. They are talking about user fees in an academic
environment. These fees are for services to members of their academic
community--and in fact, for members of their respective institutions. This
is quite different from for-profit enterprises' sphere of interest.

If the universities engage in external fee-based work, your point would tend
to have merit. But what these folks are trying to do is to spread their
cost center's burden over those who are using it, within their small
community of users.
=============================================================
I am obviously not a lawyer, however, one should always keep in mind that
Congress never granted any kind of exemptions to the anti-trust laws to tax-
exempt organizations. So George Langford is absolutely correct, none of us
should be discussing (with competitors) how we set our pricing.

Those of us who follow such things know that the penalties can be quite
severe for those who are found to be guilty of violating the anti-trust laws

From daemon Sat Dec 16 12:48:25 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 16 Dec 2000 13:46:00 -0500
Subject: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gary Gaugler wrote:
==========================================================
I can see your point of concern regarding this issue. However, if I
understand the context within these folks are discussing fees, there is a
different reference basis. They are talking about user fees in an academic
environment. These fees are for services to members of their academic
community--and in fact, for members of their respective institutions. This
is quite different from for-profit enterprises' sphere of interest.

If the universities engage in external fee-based work, your point would tend
to have merit. But what these folks are trying to do is to spread their
cost center's burden over those who are using it, within their small
community of users.
=============================================================
I am obviously not a lawyer, however, one should always keep in mind that
Congress never granted any kind of exemptions to the anti-trust laws to tax-
exempt organizations. So George Langford is absolutely correct, none of us
should be discussing (with competitors) how we set our pricing.

Those of us who follow such things know that the penalties can be quite
severe for those who are found to be guilty of violating the anti-trust laws

From daemon Sat Dec 16 15:47:36 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sun, 17 Dec 2000 10:48:24 GMT+1200
Subject: 840 success

Contents Retrieved from Microscopy Listserver Archives
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Last Friday I got my first quantitative analytical results out of the
JSM-840A which I have been converting to a 1 x eds, 3 x wds JXA
version over the past year.

This has been a big fun project, I've learned heaps, and I couldn't
have done it without this list and the help of the many listers who
have given freely of advice and suggestions, and more.

So thanks, Nestor, and all you good people out there. Please drop in
if you ever find yourselves in Auckland, New Zealand, it would be
great to be able to replace the mental images which I have of people
with factually-based ones!

cheers and happy Christmas

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Sat Dec 16 16:55:19 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 16 Dec 2000 14:48:49 -0800
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I do not agree with your analysis of this situation nor with
your conclusion. Drawing on a somewhat fading memory
of prior law classes (yes, I too am not a lawyer), I recollect
that antitrust is covered by the Sherman Antitrust Act.

The purpose of this Act and those is various states is to
prevent trusts from creating restraints on trade or commerce
and thus, reducing competition. The Sherman Antitrust Act
was designed to maintain economic liberty, and to [try to] eliminate
restraints on trade and competition.

The Act applies to all transactions and businesses involved in
interstate commerce. If the activities are local, the act applies
to transactions affecting interstate commerce. Therefore, as
I understand universities, they are not engaged in interstate
commerce. Neither are they engaged in commerce, per se.
Thus, they do not engage in transactions affecting interstate
commerce. Their fundamental basis of action is to recover
costs of ownership of university owned equipment and facilities
as applied to users of such items at the university. I do not
sense a wholesale effort by universities to engage in external
commerce. Note also that many of the managers are not
recovering the cost of their salaries. These are sunk costs
by their respective universities.

It therefore seems to me that antitrust does not apply to
universities. They are not engaged in commerce, they do
not affect interstate commerce, they do not effect
restraints on trade and commerce, and they are not
in competition with one another. And I think they would
agree that they are not in competition with private industry.

The law works in two ways: it specifies actions which are
allowed and may specify actions which are not allowed.
Lawyers exist because so much of the law is open to
interpretation. I've just provided my interpretation of the
law. Since I am not a lawyer, there is no charge for this.

gary g.





At 08:38 AM 12/16/00, you wrote:
} ------------------------------------------------------------------------
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From daemon Sat Dec 16 18:32:55 2000



From: pli-at-is.dal.ca
Date: Sat, 16 Dec 2000 20:34:50 -0400
Subject: Re: Thanks for the user fee info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I sincerely thank those of you who provided me with information and support
regarding the TEM user fee. I really appreciate it.

Regarding what "Garber, Charles A." wrote:

} .... So George Langford is absolutely correct, none of us
} should be discussing (with competitors) how we set our pricing.

I don't think this is relevant to what we have done. What we have discussed is
mostly about maintenance cost recovery, not making profit. Furthermore, we are
not competitors, and we mostly provide service for research and teaching within
our own university or institution. There is no competition among us.

Best regards,
Ping



From daemon Sat Dec 16 23:32:49 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 17 Dec 2000 03:16:47 -0800
Subject: Fwd: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary Gaugler wrote:

"It therefore seems to me that antitrust does not apply to
universities. They are not engaged in commerce, they do
not affect interstate commerce, they do not effect
restraints on trade and commerce, and they are not
in competition with one another. And I think they would
agree that they are not in competition with private industry."

Hmmm. This logic is quite interestinng. Then even though Universities
provide a service
in competition with private enterprise they are not competing. The logic in
all of this escapes me.
Hmmm.

----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Garber, Charles A." {cgarber-at-2spi.com}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, December 16, 2000 2:48 PM


I am not friendly with American law ether, but our medical school stated as
"non-profit organization". So, to me it mean, that University is not
involved in trading and thereafter is not a subject for anti-trust law.

Our hospital, for instance, charge people for the service, but they did not
make a profit, same as when we charge people for using our facilities. I
don't see any problem here as long as we did not make profit on it.

Merry Christmas! Happy New Year and New Millennium for all ListServer
readers! I wish to all of us, that our microscopes will work better in New
Millennium and we will have enough users to recover at least 70% of
maintaining cost.

Sergey.


} Date: Sat, 16 Dec 2000 14:48:49 -0800
} From: Gary Gaugler {gary-at-gaugler.com}
} Subject: Re: User fee discussion
} X-Sender: gaugler-at-pop.calweb.com (Unverified)
} To: "Garber, Charles A." {cgarber-at-2spi.com}
} Cc: MSA listserver {Microscopy-at-sparc5.microscopy.com}
} X-Mailer: QUALCOMM Windows Eudora Version 5.0
}
} ------------------------------------------------------------------------
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------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Sun Dec 17 15:59:39 2000



From: Tom Tottleben :      tomtot-at-charter.net
Date: Sun, 17 Dec 2000 15:33:05 -0600
Subject: LM Needs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Needed: Nikon Labophot 1 Epi-fluorscence lamphousing for 100w
mercury. Also interested in transformer.

Willing to trade have lots of older Zeiss 160mm items.

Thanks,
Tom




From daemon Sun Dec 17 19:10:55 2000



From: Zaluzec-at-sparc5.microscopy.com
Date: Sun, 17 Dec 2000 19:05:47 -0600
Subject: Administrivia: Reposting of a Truncated Message on User Fee's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

Chuck Garber's reply to the User Fee discussion was
truncated, due to a subtle effect of the Email system here.
In fairness I am reposting it for him so that you can
see the rest of his comments.

For those of you that are curious, the message was
truncated because of a line in the original text which
started with a "." followed by a blank/empty line. This
caused the Email system to believe the message was completed.

Nestor

Your Friendly Neighborhood SysOp

------------------------------------------------------


} From: "Garber, Charles A." {cgarber-at-2spi.com}
X-Mailer: E-Mail Connection v3.1a
Content-Length: 4627
Status:

=============================================================
I am obviously not a lawyer, however, one should always keep in mind that
Congress never granted any kind of exemptions to the anti-trust laws to tax-
exempt organizations. So George Langford is absolutely correct, none of us
should be discussing (with competitors) how we set our pricing.

Those of us who follow such things know that the penalties can be quite
severe for those who are found to be guilty of violating the anti-trust laws.

So to my (also) legally untrained eye, for universities to be discussing how
they set their pricing is no different than if independent laboratories
(like Amenex and Structure Probe) started a parallel discussion on how we
should be establishing our pricing. Anyone in academia who has ever written
a proposal for funding in recent years knows that universities are in direct
competition with each other in ways that are no different from the way
independent laboratories compete with each other. George alluded to another
issue, that is, the case when universities start offering their services to
private companies. I am not aware of anyone having gone to jail for this,
but it does raise enormous issues of ethical values, and the impact on
students, and their own views of what is right and what is wrong, when the
university's facilities are so commercialized. All of us should have some
concern about such matters.

Chuck

Disclaimer: Structure Probe, Inc. is an independent for-profit tax-paying
laboratory offering electron microscopy services to clients, often times in
direct competition with universities who claim they "don't compete with
private companies".

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
Structure Probe, Inc. /SPI Supplies FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: www.2spi.com
############################
==================================================




From daemon Mon Dec 18 07:38:51 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Mon, 18 Dec 2000 08:29:44 -0800
Subject: RE: TEM film distributers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Agfa Scientia TEM film was discontinued earlier this year. I do not know
of a manufacturer other than Kodak that is selling a "dedicated" TEM in
North America.

George Laing
National Graphic Supply

Heidi Smith wrote:


} I believe that AGFA produces a similar type of film but I do not
} know the product name or who to contact. If anyone has
} information on distributers, of either Kodak or the AGFA film,
} with reasonable prices I would appreciate hearing from you.
} Alternatively, if anyone has another favorite TEM film that they
} use and would like to share that information I would be happy to
} hear from you.
}
}



From daemon Mon Dec 18 08:08:59 2000



From: Alison Tuling :      atuling-at-postino.up.ac.za
Date: Mon, 18 Dec 2000 08:04:55 -0600
Subject: Re: LN2 and the Amazing Exploding Funnel - an alternative.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I'd like to add my 0.004$ or R0.02 to the LN2 discussion:


An alternative to both the exploding plastic funnel and freezing finger
steel funnel is to make one out of very thick brown paper (held together
with staples rather than sticky tape).

I also staple a tissue paper filter in the funnel mouth to try to remove any
ice from the LN2.


Regards
Alison Tuling

Engineer
Industrial Metal and Minerals Research Institute
Department Materials science and Metallurgical engineering
University of Pretoria
South Africa
+27 11 420 4556




From daemon Mon Dec 18 08:43:55 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 18 Dec 2000 06:39:33 -0800 (PST)
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is interesting that this discussion comes up every 12 to 18 months or so.
I also note a subtle difference in the way the question was posed, and see
that the responses have, perhaps without intending, responded to both
aspects of the original question. First, it would appear that asking for
rates, particularly at potentially competitive facilities, is at least a
gray area, if not a violation of the anti-trust laws. Second, asking
assistance in how to determine how to set rates seems to be less
problematic. It is within that context that anti-trust laws would appear to
be less applicable. For individuals who have not had to deal with the
issues of making a facility self-supporting, it can seem a daunting task.
Thus, getting advice from other facility managers who have done the task
aids the requester to determine what costs must be included, how to
determine other factors, etc, and not miss any important/essential costs.
It is important for all of us to be aware of the differences in the way
questions are posed, especially as regards to this type of issue. This has
been an enlightening discussion all around.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc

NB: These are personal opinions only, and in no way reflect any other person
or organization.

On Sat, 16 Dec 2000 21:25:04 -0800, Earl Weltmer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Gary Gaugler wrote:
}
} "It therefore seems to me that antitrust does not apply to
} universities. They are not engaged in commerce, they do
} not affect interstate commerce, they do not effect
} restraints on trade and commerce, and they are not
} in competition with one another. And I think they would
} agree that they are not in competition with private industry."
}
} Hmmm. This logic is quite interestinng. Then even though Universities
} provide a service
} in competition with private enterprise they are not competing. The logic
in
} all of this escapes me.
} Hmmm.
}
} ----- Original Message -----
} } From: "Gary Gaugler" {gary-at-gaugler.com}
} To: "Garber, Charles A." {cgarber-at-2spi.com}
} Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
} Sent: Saturday, December 16, 2000 2:48 PM
} Subject: Re: User fee discussion
}
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } I do not agree with your analysis of this situation nor with
} } your conclusion. Drawing on a somewhat fading memory
} } of prior law classes (yes, I too am not a lawyer), I recollect
} } that antitrust is covered by the Sherman Antitrust Act.
} }
} } The purpose of this Act and those is various states is to
} } prevent trusts from creating restraints on trade or commerce
} } and thus, reducing competition. The Sherman Antitrust Act
} } was designed to maintain economic liberty, and to [try to] eliminate
} } restraints on trade and competition.
} }
} } The Act applies to all transactions and businesses involved in
} } interstate commerce. If the activities are local, the act applies
} } to transactions affecting interstate commerce. Therefore, as
} } I understand universities, they are not engaged in interstate
} } commerce. Neither are they engaged in commerce, per se.
} } Thus, they do not engage in transactions affecting interstate
} } commerce. Their fundamental basis of action is to recover
} } costs of ownership of university owned equipment and facilities
} } as applied to users of such items at the university. I do not
} } sense a wholesale effort by universities to engage in external
} } commerce. Note also that many of the managers are not
} } recovering the cost of their salaries. These are sunk costs
} } by their respective universities.
} }
} } It therefore seems to me that antitrust does not apply to
} } universities. They are not engaged in commerce, they do
} } not affect interstate commerce, they do not effect
} } restraints on trade and commerce, and they are not
} } in competition with one another. And I think they would
} } agree that they are not in competition with private industry.
} }
} } The law works in two ways: it specifies actions which are
} } allowed and may specify actions which are not allowed.
} } Lawyers exist because so much of the law is open to
} } interpretation. I've just provided my interpretation of the
} } law. Since I am not a lawyer, there is no charge for this.
} }
} } gary g.
} }
} }
} }
} }
} }
} } At 08:38 AM 12/16/00, you wrote:
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} } }
} } } Gary Gaugler wrote:
} } } ==========================================================
} } } I can see your point of concern regarding this issue. However, if I
} } } understand the context within these folks are discussing fees, there
is a
} } } different reference basis. They are talking about user fees in an
} academic
} } } environment. These fees are for services to members of their academic
} } } community--and in fact, for members of their respective institutions.
} This
} } } is quite different from for-profit enterprises' sphere of interest.
} } }
} } } If the universities engage in external fee-based work, your point
would
} tend
} } } to have merit. But what these folks are trying to do is to spread
their
} } } cost center's burden over those who are using it, within their small
} } } community of users.
} } } =============================================================
} } } I am obviously not a lawyer, however, one should always keep in mind
that
} } } Congress never granted any kind of exemptions to the anti-trust laws
to
} tax-
} } } exempt organizations. So George Langford is absolutely correct, none
of
} us
} } } should be discussing (with competitors) how we set our pricing.
} } }
} } } Those of us who follow such things know that the penalties can be
quite
} } } severe for those who are found to be guilty of violating the
anti-trust
} laws
} }
} }
}
}





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Mon Dec 18 10:29:38 2000



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 18 Dec 2000 11:26:42 -0500
Subject: Re: Reposting/ User Fee's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Zaluzec-at-sparc5.microscopy.com wrote:

} Colleagues
}
} Chuck Garber's reply to the User Fee discussion was
} truncated, due to a subtle effect of the Email system here.
} In fairness I am reposting it for him so that you can
} see the rest of his comments.
} --------------------------------------------------
}
} =============================================================
} I am obviously not a lawyer, however, one should always keep in mind that
} Congress never granted any kind of exemptions to the anti-trust laws to tax-
} exempt organizations. So George Langford is absolutely correct, none of us
} should be discussing (with competitors) how we set our pricing.

What rubbish!! Of course we can discuss what standards we use to set fees.
Businesses do this all the time.

} Those of us who follow such things know that the penalties can be quite
} severe for those who are found to be guilty of violating the anti-trust laws.

Everytime someone gets on this list (and other lists) and asks about setting
prices for users of lab services, Mr Langford and/or Dr. Garber starts making
veiled threats about anti-trust violations. Isn't it interesting that no actual
statues are cited, just vague references and generalizations, and that none of
the citers are legally trained?

} So to my (also) legally untrained eye, for universities to be discussing how
} they set their pricing is no different than if independent laboratories
} (like Amenex and Structure Probe) started a parallel discussion on how we
} should be establishing our pricing. Anyone in academia who has ever written
} a proposal for funding in recent years knows that universities are in direct
} competition with each other in ways that are no different from the way
} independent laboratories compete with each other.

Not true of NIH funding of individual investigators.

} George alluded to another
} issue, that is, the case when universities start offering their services to
} private companies.

If you will read the original posting, that is NOT what was being talked
about.

} I am not aware of anyone having gone to jail for this,

I am sure that if anyone had even been formally charged, you or Mr. Langford
would be sure to let us know!

}
} but it does raise enormous issues of ethical values, and the impact on
} students, and their own views of what is right and what is wrong, when the
} university's facilities are so commercialized. All of us should have some
} concern about such matters.

Ethical behavior is a two-way street.

} Chuck
}
} Disclaimer: Structure Probe, Inc. is an independent for-profit tax-paying
} laboratory offering electron microscopy services to clients, often times in
} direct competition with universities who claim they "don't compete with
} private companies".
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} Structure Probe, Inc. /SPI Supplies FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
} Look for us!
} ############################
} WWW: www.2spi.com
} ############################
} ==================================================

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Mon Dec 18 13:13:14 2000



From: Tom Pella :      tom_pella-at-tedpella.com
Date: Mon, 18 Dec 2000 11:07:47 -0800
Subject: Re: Film dryers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Regarding the contact info that Scott mentioned awhile back, we happen
to sell what is
probably that film dryer. You can see it at

http://www.tedpella.com/photo_html/photo11.htm

Tom

"Walck, Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} There is a company called California Stainless that made film dryers that
} some of the EM supply houses sold. I bought one from them a number of years
} ago. Sorry that I don't have the contact info, but I'm sure that this is
} just what you want.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}
} -----Original Message-----
} From: Donald Lovett [mailto:lovett-at-tcnj.edu]
} Sent: Monday, November 20, 2000 9:51 AM
} To: Microscopy.lst
} Subject: Film dryers
}
}
} ---------------------------------------------------------------
} ---------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html
}
}
}
} ---------------------------------------------------------------
} --------.
}
}
}
} Years ago I worked in a lab that had a small forced (and filtered) film
} dryer (with heater). It was just large enough to hold two racks of TEM
} negatives. I cannot find one in any of the catalogues. I can
} only find
} film dryers for 35 mm roll film. Could anyone please
} recommend a source
} of a similar dryer? (Vendors, please feel free to respond directly to
} me).
}
} Thanks,
}
} Don
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-tcnj.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} P.O. Box 7718 fax: (609) 637-5118
} The College of New Jersey
} Ewing, NJ 08628-0718
}
}
}
}


From daemon Mon Dec 18 14:27:42 2000



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 18 Dec 2000 15:21:30 -0500
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Roger Moretz wrote:

} It is interesting that this discussion comes up every 12 to 18 months or so.

Yes. Some listmember asks for a little guidance and get several replys. Then two
people (the same two people, if my memory serves me) start yelling "anti-trust
violation". No facts, no applicable case law, just the suggestion of
lawlessness.

} I also note a subtle difference in the way the question was posed, and see
} that the responses have, perhaps without intending, responded to both
} aspects of the original question. First, it would appear that asking for
} rates, particularly at potentially competitive facilities, is at least a
} gray area, if not a violation of the anti-trust laws.

How? Please tell us exactly how asking what some other lab charges for XYZ is
illegal.

} Second, asking
} assistance in how to determine how to set rates seems to be less
} problematic. It is within that context that anti-trust laws would appear to
} be less applicable. For individuals who have not had to deal with the
} issues of making a facility self-supporting, it can seem a daunting task.
} Thus, getting advice from other facility managers who have done the task
} aids the requester to determine what costs must be included, how to
} determine other factors, etc, and not miss any important/essential costs.
} It is important for all of us to be aware of the differences in the way
} questions are posed, especially as regards to this type of issue. This has
} been an enlightening discussion all around.
}
} Roger Moretz, Ph.D.
} Dept of Toxicology
} Boehringer Ingelheim Pharmaceuticals, Inc
}
} NB: These are personal opinions only, and in no way reflect any other person
} or organization.
}
} On Sat, 16 Dec 2000 21:25:04 -0800, Earl Weltmer wrote:
}
} } Gary Gaugler wrote:
} }
} } "It therefore seems to me that antitrust does not apply to
} } universities. They are not engaged in commerce, they do
} } not affect interstate commerce, they do not effect
} } restraints on trade and commerce, and they are not
} } in competition with one another. And I think they would
} } agree that they are not in competition with private industry."
} }
} } Hmmm. This logic is quite interestinng. Then even though Universities
} } provide a service
} } in competition with private enterprise they are not competing. The logic
} in
} } all of this escapes me.
} } Hmmm.
} }
} } ----- Original Message -----
} } } From: "Gary Gaugler" {gary-at-gaugler.com}
} } To: "Garber, Charles A." {cgarber-at-2spi.com}
} } Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
} } Sent: Saturday, December 16, 2000 2:48 PM
} } Subject: Re: User fee discussion
} }
} }
} } } I do not agree with your analysis of this situation nor with
} } } your conclusion. Drawing on a somewhat fading memory
} } } of prior law classes (yes, I too am not a lawyer), I recollect
} } } that antitrust is covered by the Sherman Antitrust Act.
} } }
} } } The purpose of this Act and those is various states is to
} } } prevent trusts from creating restraints on trade or commerce
} } } and thus, reducing competition. The Sherman Antitrust Act
} } } was designed to maintain economic liberty, and to [try to] eliminate
} } } restraints on trade and competition.
} } }
} } } The Act applies to all transactions and businesses involved in
} } } interstate commerce. If the activities are local, the act applies
} } } to transactions affecting interstate commerce. Therefore, as
} } } I understand universities, they are not engaged in interstate
} } } commerce. Neither are they engaged in commerce, per se.
} } } Thus, they do not engage in transactions affecting interstate
} } } commerce. Their fundamental basis of action is to recover
} } } costs of ownership of university owned equipment and facilities
} } } as applied to users of such items at the university. I do not
} } } sense a wholesale effort by universities to engage in external
} } } commerce. Note also that many of the managers are not
} } } recovering the cost of their salaries. These are sunk costs
} } } by their respective universities.
} } }
} } } It therefore seems to me that antitrust does not apply to
} } } universities. They are not engaged in commerce, they do
} } } not affect interstate commerce, they do not effect
} } } restraints on trade and commerce, and they are not
} } } in competition with one another. And I think they would
} } } agree that they are not in competition with private industry.
} } }
} } } The law works in two ways: it specifies actions which are
} } } allowed and may specify actions which are not allowed.
} } } Lawyers exist because so much of the law is open to
} } } interpretation. I've just provided my interpretation of the
} } } law. Since I am not a lawyer, there is no charge for this.
} } }
} } } gary g.
} } }
} } } }
} } } }
} } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} } } }
} } } } Gary Gaugler wrote:
} } } } ==========================================================
} } } } I can see your point of concern regarding this issue. However, if I
} } } } understand the context within these folks are discussing fees, there
} is a
} } } } different reference basis. They are talking about user fees in an
} } academic
} } } } environment. These fees are for services to members of their academic
} } } } community--and in fact, for members of their respective institutions.
} } This
} } } } is quite different from for-profit enterprises' sphere of interest.
} } } }
} } } } If the universities engage in external fee-based work, your point
} would
} } tend
} } } } to have merit. But what these folks are trying to do is to spread
} their
} } } } cost center's burden over those who are using it, within their small
} } } } community of users.
} } } } =============================================================
} } } } I am obviously not a lawyer, however, one should always keep in mind
} that
} } } } Congress never granted any kind of exemptions to the anti-trust laws
} to
} } tax-exempt organizations. So George Langford is absolutely correct, none
} of us
} } } } should be discussing (with competitors) how we set our pricing.
} } } }
} } } } Those of us who follow such things know that the penalties can be
} quite
} } } } severe for those who are found to be guilty of violating the
} anti-trust
} } laws
}
} _______________________________________________________
} Send a cool gift with your E-Card
} http://www.bluemountain.com/giftcenter/

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Mon Dec 18 15:15:56 2000



From: Marilyn Howton :      mhowton-at-hsc.wvu.edu
Date: Mon, 18 Dec 2000 16:12:27 -0500
Subject: Re:LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ah yes, this reminds me of a similar Close Encounter with liq N2 that I had:
I removed a stainless steel rack from a Dewar, the type used for holding 5"X5" boxes as a cell freezer. Heavy, and dripping liq N2, I placed it down on one of those gray round rubber-topped step stools found in most labs. It was quite exciting when the rubber exploded with a CRACK and sent shards of frozen rubber in every direction. Luckily, I was alone and never 'fessed up to the missing rubber top on the step stool..............
Marilyn



From daemon Mon Dec 18 16:06:20 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 18 Dec 2000 21:53:29 -0000
Subject: F F: FW: Fwd: Fwded: Forwarded: Seasons Greetings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

Best wishes for an environmentally conscious, socially responsible,
low stress, non-addictive, gender neutral, winter solstice holiday,
practiced within the most joyous traditions of the religious
persuasion of your choice, but with due respect for the religious
persuasion of others who choose to practice their own religion, as
well as those who choose not to practice a religion at all.

Additionally, (topical bit here!) a fiscally successful, personally
fulfilling, and medically uncomplicated recognition of the generally
accepted calendar year 2001, but not without due respect for the
alternative calendars of choice of other cultures whose contributions
have helped make our society great, without regard to the race,
creed, color, religious, or sexual preferences of the wishes.

May the deity of our choice bless us each and every one but should
you not be a believer (as above) then please accept our collective
wish as fellow sentient (?) beings for the full development of your
inner self.

Yours,
Chris
==================================================

(Disclaimer: This greeting is subject to confirmation, clarification or
withdrawal. It implies no promise by the sendeer actually to
implement any of the expressed wishes for her/himself or others and
no responsibility for any unintended emotional stress these
greetings may or may not bring to those not caught up in the holiday
spirit.)

Hope y'all manage to get a life this Christmas
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Mon Dec 18 16:06:25 2000



From: Zhiping LUO :      luo-at-msd.anl.gov
Date: 18 Dec 00 15:44:32 -0800
Subject: RE: Fwd: Re: MRS follow-up

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From: Zhiping LUO :      luo-at-msd.anl.gov
Date: 18 Dec 00 15:44:32 -0800
Subject: RE: Fwd: Re: MRS follow-up

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Reply to: RE: Fwd: Re: MRS follow-up

Dear Experts of Quasicrystals,

Recently I received two e-mails from the MRS QC meeting organizer to this
entire group of recipients I am addressing, concerning the binary QCs.
Since the binary QCs are interesting, I would like to make some more
discussions.

In the literature, many binary QCs have been observed, including the Al-,
Cd-, Ti-, and Cr-based binary systems (to see Pr. Guyot's review, Rep.
Prog. Phys., 1991, 54, p. 1373, Table 1). The Ta- and Ga-based systems found
later are not listed there. Some of these constituents may be substituted
by other elements, not only for the Cd-Cu system (Bendersky et al.,
Scripta metall., 1987, 21, 531) but also for other alloy systems. Since the
substitution seems to be relatively easier in binary alloys, probably there
are some more chances to explore new QC alloys by the substitution method
(little changes in composition maybe needed), Among those QCs observed so
far, some may still remain unclear if they can form from the liquid
directly or not.

The interesting thing to us is that why some QCs can form from the liquid
directly. However, even if a QC forms directly from the liquid, it
doesn't mean that it must be a stable phase (for example, we recently observed
that the Mg-Zn-RE IQCs are not thermodynamically stable). Therefore, if a
QC is not thermodynamically stable, it should be more accurately renamed as
an "as-cast" QC instead of a "stable" QC.

With best regards.

Z. P. Luo




From daemon Mon Dec 18 16:53:13 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Mon, 18 Dec 2000 14:49:50 -0800
Subject: Polaroid Model 550 film holder

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Hi everyone,

thanks for the deluge of TEM film holders - we have plenty now thanks.

Yet another plea - last week we noticed we were suddenly getting nasty
streaks on our SEM photos from light getting to our film holder. Close
examination revealed that the plastic casing has cracked through.

In light of the fact that lots of people are going digital with their SEM
images, does anyone have an old 550 Polaroid film holder that they are
willing to sell to us? If not, we are willing to sell off our 30 packs of
polaplan film that goes in this type of holder.

cheers
Liz McKenzie

*******************************************************
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025

*******************************************************


From daemon Mon Dec 18 17:19:17 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Tue, 19 Dec 2000 10:14:51 +1100
Subject: Re: F F: Seasons Greetings

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I must protest the blatant and insensitive hemispherism implicit in this
posting.

Merry er....just be happy, OK?

Sally Stowe
(Canberra, Australia, where its 30 degrees and rising).





Chris Jeffree wrote:

---

Dear All

Best wishes for an environmentally conscious, socially responsible,
low stress, non-addictive, gender neutral, winter solstice holiday,
practiced within the most joyous traditions of the religious
persuasion of your choice, but with due respect for the religious
persuasion of others who choose to practice their own religion, as
well as those who choose not to practice a religion at all.
...........
etc


From daemon Mon Dec 18 19:56:38 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Mon, 18 Dec 2000 18:49:51 -0600
Subject: Anti-trust laws

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Folks, this is the year 2000. Anti trust laws dont mean, nor do diddly
squat. It is all rhetoric spun off to make it look like things are peachy
keen. In reality it is pure B_ _ _S_ _T. Same for when the gurus talk
about how great the economy is. Look into your own wallet and assess if
you are REALLY doing better than you were 10-20 year ago. The answer is
NOT. Lou Solebello




From root Mon Dec 18 20:28:14 2000
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Folks, this is the year 2000. Anti trust laws dont mean, nor do diddly
squat. It is all rhetoric spun off to make it look like things are peachy
keen. In reality it is pure B_ _ _S_ _T. Same for when the gurus talk
about how great the economy is. Look into your own wallet and assess if
you are REALLY doing better than you were 10-20 year ago. The answer is
NOT. Lou Solebello




From daemon Mon Dec 18 20:28:21 2000



From: Ardlev-at-aol.com
Date: Mon, 18 Dec 2000 20:14:41 -0600
Subject: Viewing water in oil emulsion drops using a SEM or TEM

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To anyone who has experience in attempting to view and determine emulsions
bead size using a SEM and determining average particle size of a past
wax/paint sealant.

Please respond to Thomas Van Doozer tvd-at-gvtc.com
Thank you.




From daemon Tue Dec 19 01:11:33 2000



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 18 Dec 2000 11:26:42 -0500
Subject: Re: Reposting/ User Fee's

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Zaluzec-at-sparc5.microscopy.com wrote:

} Colleagues
}
} Chuck Garber's reply to the User Fee discussion was
} truncated, due to a subtle effect of the Email system here.
} In fairness I am reposting it for him so that you can
} see the rest of his comments.
} --------------------------------------------------
}
} =============================================================
} I am obviously not a lawyer, however, one should always keep in mind that
} Congress never granted any kind of exemptions to the anti-trust laws to tax-
} exempt organizations. So George Langford is absolutely correct, none of us
} should be discussing (with competitors) how we set our pricing.

What rubbish!! Of course we can discuss what standards we use to set fees.
Businesses do this all the time.

} Those of us who follow such things know that the penalties can be quite
} severe for those who are found to be guilty of violating the anti-trust laws.

Everytime someone gets on this list (and other lists) and asks about setting
prices for users of lab services, Mr Langford and/or Dr. Garber starts making
veiled threats about anti-trust violations. Isn't it interesting that no actual
statues are cited, just vague references and generalizations, and that none of
the citers are legally trained?

} So to my (also) legally untrained eye, for universities to be discussing how
} they set their pricing is no different than if independent laboratories
} (like Amenex and Structure Probe) started a parallel discussion on how we
} should be establishing our pricing. Anyone in academia who has ever written
} a proposal for funding in recent years knows that universities are in direct
} competition with each other in ways that are no different from the way
} independent laboratories compete with each other.

Not true of NIH funding of individual investigators.

} George alluded to another
} issue, that is, the case when universities start offering their services to
} private companies.

If you will read the original posting, that is NOT what was being talked
about.

} I am not aware of anyone having gone to jail for this,

I am sure that if anyone had even been formally charged, you or Mr. Langford
would be sure to let us know!

}
} but it does raise enormous issues of ethical values, and the impact on
} students, and their own views of what is right and what is wrong, when the
} university's facilities are so commercialized. All of us should have some
} concern about such matters.

Ethical behavior is a two-way street.

} Chuck
}
} Disclaimer: Structure Probe, Inc. is an independent for-profit tax-paying
} laboratory offering electron microscopy services to clients, often times in
} direct competition with universities who claim they "don't compete with
} private companies".
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} Structure Probe, Inc. /SPI Supplies FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
} Look for us!
} ############################
} WWW: www.2spi.com
} ############################
} ==================================================

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Tue Dec 19 06:51:59 2000



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Tue, 19 Dec 2000 14:06:27 +0100 (CAT)
Subject: Interpretation of Thin film Micrographs

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Hi guys,
Anybody who can give me tips on interpretation of thin film micrographs.
Or where I can get info on the same subject on the net.
Thanks for all your help.

Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.




From daemon Tue Dec 19 08:39:04 2000



From: Kim, Hyung Tae (NINDS) :      KimTH-at-ninds.nih.gov
Date: Tue, 19 Dec 2000 09:09:06 -0500
Subject: LM-need help low-power photomicrographs for negative images

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Dear Colleagues:

We are trying to take the low-power photomicrographs for negative images to
identify the connections past the interstitial subnucleus in the Nucleus
tractus solitarius by neuronal tracer.
Any guidance on this would be very much appreciated
Thank you in advance.


Hyung-Tae Kim

Postdoctoral Fellow
Laryngeal and Speech Section, MNB, NINDS
Bldg. 10 Rm 5D38
10 Center Drive, MSC 1416
Bethesda, MD 20892-1416

email : kimth-at-ninds.nih.gov
Phone : 301-402-0157
Fax : 301-480-0803


From daemon Tue Dec 19 08:39:05 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 19 Dec 2000 09:36:21 -0500
Subject: Re: cryprotectants

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At 3:27 PM -0500 12/15/00, Shea Miller wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Dec 19 09:23:00 2000



From: Tim Richardson :      mirlyn-at-attglobal.net
Date: Tue, 19 Dec 2000 10:20:26 -0500
Subject: F F: FW: Fwd: Fwded: Forwarded: Seasons Greetings

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Dear Raymond,

Here is the whole greeting!

Tim

================


Dear All

Best wishes for an environmentally conscious, socially responsible,
low stress, non-addictive, gender neutral, winter solstice holiday,
practiced within the most joyous traditions of the religious
persuasion of your choice, but with due respect for the religious
persuasion of others who choose to practice their own religion, as
well as those who choose not to practice a religion at all.

Additionally, (topical bit here!) a fiscally successful, personally
fulfilling, and medically uncomplicated recognition of the generally
accepted calendar year 2001, but not without due respect for the
alternative calendars of choice of other cultures whose contributions
have helped make our society great, without regard to the race,
creed, color, religious, or sexual preferences of the wishes.

May the deity of our choice bless us each and every one but should
you not be a believer (as above) then please accept our collective
wish as fellow sentient (?) beings for the full development of your
inner self.

Yours,
Chris
==================================================

(Disclaimer: This greeting is subject to confirmation, clarification or
withdrawal. It implies no promise by the sendeer actually to
implement any of the expressed wishes for her/himself or others and
no responsibility for any unintended emotional stress these
greetings may or may not bring to those not caught up in the holiday
spirit.)

Hope y'all manage to get a life this Christmas
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


===============================================================
Tim Richardson, R&D, Bio-Microtech Inc. & Bolton Bio-Research
email: mirlyn-at-attglobal.net, web: www.bio-microtech.com
ph: 905-951-7058 fax: 905-951-7052
4-670 Hardwick Road, P.O. Box 23, Bolton, Ontario, L7E 5T1, Canada





From daemon Tue Dec 19 11:03:51 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 19 Dec 2000 16:50:34 -0000
Subject: (Fwd) Re: F F: FW: Fwd: Fwded: Forwarded: Seasons Greetings

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Thanks for your all your replies on this topic. Some liked it, and
some didn't but there was a substantial number of spoiled ballots, so
the jury is still out. Best wishes to you all anyway!
Chris
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Tue Dec 19 12:21:29 2000



From: michael shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 19 Dec 2000 10:16:38 -0800
Subject: EPMA+SEM: position announcement

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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
University of Oregon
EPMA/SEM Technician
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

The Department of Geological Sciences at the University
of Oregon invites applications for an electron beam specialist
in a modern microanalytical facility. The facility houses a
four-spectrometer Cameca SX-50 microprobe and a JEOL JSM-6300
SEM. It serves faculty and students in the Department of
Geological Sciences, Chemistry, Physics and the Materials
Science Institute. Primary responsibilities include instrument
maintenance, instruction and research collaboration.
Additional responsibilities include maintenance and operation
of support and sample preparation equipment as well as optical
microscopes and a network of computers used for file archival
and data/image analysis.

We seek an individual with expertise and experience in
WDS and EDS analytical techniques, SEM imaging and instrument
maintenance. Additional desired skills include experience
with network computers (UNIX/Windows), applied computer-aided
microscopy and instruction in instrument use. An advanced
degree in petrology, mineralogy or analytical chemistry is
desirable.

This is a full-time, annually renewable position.
Salary and title will be commensurate with experience and
education. Applicants should send curriculum vitae, a
statement of experience and interests, and the names,
addresses, phone numbers and email addresses of at least
three referees to the EPMA/SEM Search Committee, Department
of Geological Sciences, 1272 University of Oregon,
Eugene OR 97403-1272. We will begin reviewing completed
applications February 1, 2001, and will continue until the
position is filled.

The University of Oregon is an equal
opportunity/affirmative action institution committed to
cultural diversity and compliance with the Americans
with Disabilities Act.

A PDF of the text is available ... we would
genuinely appreciate this announcement's distribution.
http://epmalab.uoregon.edu/epma_position.pdf

Feel free to visit the facility's wwwsite for more
information regarding the Dep't of Geological Sciences,
the University of Oregon, and the community of Eugene.
http://epmalab.uoregon.edu/ ...

to ask any specific questions ...
about the facility: mailto:epmalab-at-darkwing.uoregon.edu
about the position: Jack Rice mailto:jrice-at-darkwing.uoregon.edu

... and a special thanx to my peers ...
http://epmalab.uoregon.edu/images/zircon-xmas.jpg

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/


From daemon Tue Dec 19 13:47:22 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 19 Dec 2000 14:45:18 -0500
Subject: Re: Reposting/ User Fee's

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Some key points to the commercial guys....

We at university-based facilities are not competing with commercial
labs, nor are we out to steal any business away from you. Believe
me, most of us have our hands full trying to keep up with the
needs/demands of our in-house clients. We are here to provide
service at reasonable cost to the research faculty, students, and
staff. I know that my facility is expected to cover its
non-personnel operating costs (service contracts, supplies,
maintenance contracts) and NOT turn a profit. That is against NIH
guidelines (take a look at the tapes/transcripts of the M&M sessions
devoted to facility management). Since most if not all of my clients
are federally funded, I am not allowed to charge them more than a fee
calculated to cover my expenses for the procedure involved. My
institution maintains a separate EM facility for clinical
applications, in part because of the billing differences.
I think that the original question posted was a plea for help in
establishing de novo an approximation of what is a reasonable
price....high enough to cover operating costs and keep from operating
too far "in the red", but not so high as to be unreasonable or out of
the range for people operating within grant budgets.

It is not unreasonable for people to want to be able to get work done
without having to send it out or schlepp it half-way across town. It
has become too costly for individuals to maintain this type of
equipment in their own labs, and many people need access only on a
sporadic basis. That is why central core facilities get set up.


Chill out guys.

Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Dec 19 14:44:34 2000



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 19 Dec 2000 15:43:20 -0500
Subject: Re: LM-need help low-power photomicrographs for negative images

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"Kim, Hyung Tae (NINDS)" wrote:

} Dear Colleagues:
}
} We are trying to take the low-power photomicrographs for negative images to
} identify the connections past the interstitial subnucleus in the Nucleus
} tractus solitarius by neuronal tracer.
} Any guidance on this would be very much appreciated
} Thank you in advance.
}
}
} Hyung-Tae Kim
}
} Postdoctoral Fellow
} Laryngeal and Speech Section, MNB, NINDS
} Bldg. 10 Rm 5D38
} 10 Center Drive, MSC 1416
} Bethesda, MD 20892-1416
}
} email : kimth-at-ninds.nih.gov
} Phone : 301-402-0157
} Fax : 301-480-0803

Could you be more specific about the problems you are encountering? What is
keeping you from getting useful images?


Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Dec 19 16:16:24 2000



From: sghoshro-at-nmsu.edu
Date: Tue, 19 Dec 2000 15:12:27 -0700 (MST)
Subject: control unit for Ultracut

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I am looking for a used control unit for Reichert Ultracut Ultramicrotome.
This is a separate box sits right next to the main microtome and some key
functions of the microtome can be controlled from it. If anyone has one
for sale, I will be very interested in buying it. Please contact me
directly, not to the listserve.

Thank you,

Soumitra



*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Tue Dec 19 17:39:52 2000



From: Geinfam-at-aol.com
Date: Tue, 19 Dec 2000 18:35:46 EST
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com


From daemon Tue Dec 19 18:56:01 2000



From: Michael Nesson :      nessonm-at-ucs.orst.edu
Date: Tue, 19 Dec 2000 16:51:55 -0800
Subject: Wanted:Objectives for Zeiss SV8.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Help!
I'm looking for objective lenses for a Zeiss STEMI SV8 stereomicroscope.

The Zeiss part numbers include: 47 50 73 S 50 mm.
47 50 61 S
100 mm.
47 50 62 PLS
100 mm.
47 50 63 DS
100 mm.
47 50 72
Photo S 100 mm.
47 50 74 S
150 mm.
and 47 50 71 S 200
mm.

Please respond to me directly at nessonm-at-ucs.orst.edu with prices and
condition.
DON'T just hit the REPLY button and bother everyone else in the
newsgroup.

TIA,
M. Nesson
_______________________________________________________________________
Michael Nesson, Ph.D. Department of Biochemistry & Biophysics
2011 Ag&LS, Oregon State University, Corvallis, OR 97331-7305
(541)737-5245 FAX:(541)737-0481 nessonm-at-ucs.orst.edu




From daemon Tue Dec 19 19:49:00 2000



From: hadden-at-wingate.edu (Lee Hadden)
Date: Tue, 19 Dec 2000 20:47:47 -0500
Subject: RCA EMU 3G TEM

Contents Retrieved from Microscopy Listserver Archives
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How might I find out if there is anything we can do with our old RCA
EMU3G TEM other than simply scrapping it? How could I list it where
someone who might need it for parts would see it? It was working until
the day we stopped teaching the course here in 1990, and with the
arrival of our new SEM in January the old TEM has to go to make room for
it.

Thanks for any assistance.

Sincerely,
Lee Hadden
Professor of Biology
Department of Biology
Wingate University
Wingate, NC 28174

hadden-at-wingate.edu
http://www.wingate.edu
704-233-8238



From daemon Tue Dec 19 22:12:39 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 19 Dec 2000 20:56:52 -0700
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
date.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 4:36 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com


From daemon Wed Dec 20 02:38:49 2000



From: Feng Wu :      fwu-at-gbumail.bgu.ac.il
Date: Wed, 20 Dec 2000 10:34:51 +0200
Subject: preparation TEM sample of coral

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anybody tell me how to prepare TEM samples for biomaterials such as
coral?
Thanks.
Feng
**************************************************
Dr. Feng Wu
Dept. of Materials Engineering
Ben-Gurion University of the Negev
Beer-Sheva 84105, Israel

************************************************



From daemon Wed Dec 20 03:32:30 2000



From: Erwin Decraen :      erwin.decraen-at-bbri.be
Date: Wed, 20 Dec 2000 10:29:26 +0100
Subject: RE: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Philips delivered their first commercial electron microcope in October 1949
(bought by 'Statens Serum Institute' in Denmark for research into the polio
virus)

Source: FEI Company - Scope 11 - December 1999 - page 3

Decraen Erwin

Belgian Building Research Institute (BBRI)
Division of Materials
Av. P. Holoffe 21
B - 1342 Limelette

Tel. : + 32 (0)2 655 77 11
Fax : + 32 (0)2 653 07 29
E-mail : erwin.decraen-at-bbri.be {mailto:erwin.decraen-at-bbri.be}


-----Original Message-----
From: Ekstrom, Harry [SMTP:harry.ekstrom-at-honeywell.com]
Sent: Wednesday, December 20, 2000 4:57 AM
To: '"Geinfam-at-aol.com"-at-sparc5.microscopy.com';
Microscopy-at-sparc5.microscopy.com
Subject: RE: Electron microscope first demonstration


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My source quotes M.Knoll first demonstrated the SEM in 1935 but no
specific
date.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 4:36 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: Electron microscope first demonstration



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


Can anyone tell me the exact date in the "30's when the electron
microscope
was first demonstrated?

geinfam-at-aol.com


From daemon Wed Dec 20 04:42:53 2000



From: Hyman, S.C. :      sch10-at-leicester.ac.uk
Date: Wed, 20 Dec 2000 10:18:28 -0000
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
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I have a date of Tues 07 April 1931 for the first two-stage (magnification
of an electron image further magnified by a second lens) image of a platinum
grid being obtained by Ruska.

Ref. T. Mulvey, Dept. Physics, The University of Aston, Birmingham. - An
article based upon the opening address of the fifth European congress on
electron microscopy, EMCON 72 held in Manchester 5-12 September 1972.



S.C. Hyman
Chief Technician
The Electron Microscope Laboratory
Faculty of Medicine and Biological Sciences
Adrian Building
University of Leicester
University Road
Leicester
LE1 7RH

Tel. (0116) 252 3370




-----Original Message-----
} From: Ekstrom, Harry [mailto:harry.ekstrom-at-honeywell.com]
Sent: 20 December 2000 03:57
To: '"Geinfam-at-aol.com"-at-sparc5.microscopy.com';
Microscopy-at-sparc5.microscopy.com


My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
date.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 4:36 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com


From daemon Wed Dec 20 06:17:36 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 20 Dec 2000 08:12:04 -0400
Subject: Re: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Can anyone tell me the exact date in the "30's when the electron
microscope
} was first demonstrated?

I have a little brochure in front of me called "The First North American
Electron Microscope", prepared by U.M. Franklin, G.C. Weatherly and G.T.
Simon, in 1978. In a section about the early European design efforts, they
state that Ruska had "constructed the pioneer two-stage transmission model
in 1933", but don't give any further information on it.

Frank Thomas
Geological Survey of Canada Atlantic
Dartmouth, Nova Scotia
Canada



From daemon Wed Dec 20 06:54:33 2000



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Wed, 20 Dec 2000 07:51:48 -0500
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
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I believe that Ernst Ruska invented the transmission electron microscope in
1931.




"Geinfam/-at-aol.com" on 12/19/2000 06:35:46 PM





To: Microscopy-at-sparc5.microscopy.com
cc:


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com






From daemon Wed Dec 20 06:56:04 2000



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Wed, 20 Dec 2000 06:53:50 -0600
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My guess would be Ernst Ruska with the first electron micrograph in April
1931.
Hope this helps.

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 5:36 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com


From daemon Wed Dec 20 06:58:05 2000



From: jshields-at-cb.uga.edu
Date: Wed, 20 Dec 2000 08:00:51 -0500
Subject: SouthEastern Microscopy Society meeting info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello!
This is to inform members and other interested individuals that the
newsletter for the Southeastern Microscopy Society is now online
at http://www.biotech.ufl.edu/sems/ . This issue contains
information about our annual meeting to be held May 23-25, 2001
at Clemson University.
The issue also includes information and forms for joining the
Society.
Thanks
John P. Shields, PhD
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602
706-542-4080
FAX 706-542-4271
jshields-at-cb.uga.edu


From daemon Wed Dec 20 08:06:23 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Wed, 20 Dec 2000 23:55:30 +1000
Subject: RE: preparation TEM sample of coral

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eragonite or polyp? Hard or soft?
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, December 20, 2000 6:35 PM, Feng Wu [SMTP:fwu-at-gbumail.bgu.ac.il]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anybody tell me how to prepare TEM samples for biomaterials such as
} coral?
} Thanks.
} Feng
} **************************************************
} Dr. Feng Wu
} Dept. of Materials Engineering
} Ben-Gurion University of the Negev
} Beer-Sheva 84105, Israel
}
} ************************************************
}



From daemon Wed Dec 20 08:06:28 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Wed, 20 Dec 2000 23:53:05 +1000
Subject: RE: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
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In 1935? That was a TEM, not an SEM. Knoll was Ernst Ruska's PhD supervisor and
later collaborator. They designed and built the first TEMs.
The very first model had no specimen chamber and just proved that the lens
system could work. So the first model, which I seem to remember was before 1935
was really not a microscope.
Von Ardenne described the operating principle of an SEM in the late 30th, but I
believe none was build until the early 60th at Cambridge University, UK.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, December 20, 2000 1:57 PM, Ekstrom, Harry
[SMTP:harry.ekstrom-at-honeywell.com] wrote:
}
}
} My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
} date.
}
} Harry Ekstrom
} Materials Laboratory
}
} (602) 231-2744
} e-mail: harry.ekstrom-at-honeywell.com
}
}
} -----Original Message-----
} } From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Tuesday, December 19, 2000 4:36 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Electron microscope first demonstration
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone tell me the exact date in the "30's when the electron microscope
} was first demonstrated?
}
} geinfam-at-aol.com
}



From daemon Wed Dec 20 09:27:40 2000



From: Robert Blystone :      rblyston-at-trinity.edu
Date: Wed, 20 Dec 2000 09:23:01 -0600
Subject: EM invention

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the list:

Concerning the invention of the electron microscope. The references
below answer the question.

Check the following Web sites.

http://mail.plymouth.edu/~biology/history/ruska.html

http://www.medhelpnet.com/medhist8.html

The reference below is definitive. A must read.
http://www.nobel.se/physics/laureates/1986/ruska-autobio.html

http://www.britannica.com/bcom/eb/article/5/0%2C5716%2C66125%201%2064460%2C
00.html

Ernst had a brother and they published together.
http://www.findarticles.com/cf_0/m0833/9216_355/62115190/p1/article.jhtml


Bob Blystone

Robert V. Blystone, Ph.D.
Professor of Biology
Trinity University
San Antonio, Texas 78212
rblyston-at-trinity.edu
210-999-7243 FAX 210-999-7229



From daemon Wed Dec 20 09:48:41 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 20 Dec 2000 10:37:59 -0500 (EST)
Subject: RE: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think Ernst Ruska is generally credited for building the protootype of
today's microscope as his PhD project. He and Max Knoll published a
description in 1932 (Knoll M, Ruska E. The electron microscope. Z Physik
78:318-39, 1932.).

However, others contributed to the design and knowledge of electron
behavior and lenses, including de Broglie on wave theory (Phil Mag
47:446-58, 1924) and Busch on lenses (Ann Physik 15 (Ser 5):145-66,
1932). Also Rudenberg received patents describing and EM in 1932, and
Bruche and Johanson built the first electrostatic EM that could enlarge
images of self-luminous objects in 1932.

The first commercial EM was developed by von Borries and Ruska and
produced in Germany by Siemens and Halske in 1939, and the first
commercial EM in the US was the RCA developed by Hillier, Vance, and some
others.

This is probably more than you wanted to know, but "the" electron
microscope was really a series of developments, and today's microscopes
benefitted from additional contributions and refinements of others.

Hope this helps.
Sara Miller



On Tue, 19 Dec 2000, Ekstrom, Harry wrote:

} Date: Tue, 19 Dec 2000 20:56:52 -0700
} From: Ekstrom, Harry {harry.ekstrom-at-honeywell.com}
} To: "'\"Geinfam-at-aol.com\"-at-sparc5.microscopy.com'"
{"Geinfam-at-aol.com"-at-sparc5.microscopy.com} ,
} Microscopy-at-sparc5.microscopy.com
} Subject: RE: Electron microscope first demonstration
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
} date.
}
} Harry Ekstrom
} Materials Laboratory
}
} (602) 231-2744
} e-mail: harry.ekstrom-at-honeywell.com
}
}
} -----Original Message-----
} } From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Tuesday, December 19, 2000 4:36 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Electron microscope first demonstration
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone tell me the exact date in the "30's when the electron microscope
} was first demonstrated?
}
} geinfam-at-aol.com
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Dec 20 10:24:51 2000



From: Erdem Yasar :      yasar-at-turkuaz.kku.edu.tr
Date: Wed, 20 Dec 2000 18:11:42 +0200 (EET)
Subject: About tem specimen

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir/Madam,
Can anybody tell me how to prepare TEM samples for Single crystal?
Thanks for your interested




**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************








From daemon Wed Dec 20 10:35:58 2000



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Wed, 20 Dec 2000 10:32:12 -0600
Subject: RE: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
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An excellent, brief history of EM development, written by Ernst Ruska
himself (as the text of his Nobel Prize address) appears in Reviews of
Modern Physics Vol. 59 No. 3 Part I p.p. 627-638 (1987). It includes some
interesting photos, micrographs, and drawings

To answer the question, in this article Ruska states that he and Max Knoll
first demonstrated magnified electron images of a specimen on 7 April 1931.
...........................................................................
......................................................
Jeffrey A. Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439-4837

(630) 252-5594 (voice)
(630) 252-4771 (fax)



} ----------
} From: Jim at ProSciTech
} Reply To: jim-at-proscitech.com
} Sent: Wednesday, December 20, 2000 7:53 AM
} To: 'Ekstrom, Harry'; '"Geinfam-at-aol.com"-at-sparc5.microscopy.com';
} Microscopy-at-sparc5.microscopy.com
} Subject: RE: Electron microscope first demonstration
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} In 1935? That was a TEM, not an SEM. Knoll was Ernst Ruska's PhD
} supervisor and
} later collaborator. They designed and built the first TEMs.
} The very first model had no specimen chamber and just proved that the lens
}
} system could work. So the first model, which I seem to remember was before
} 1935
} was really not a microscope.
} Von Ardenne described the operating principle of an SEM in the late 30th,
} but I
} believe none was build until the early 60th at Cambridge University, UK.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, December 20, 2000 1:57 PM, Ekstrom, Harry
} [SMTP:harry.ekstrom-at-honeywell.com] wrote:
} }
} }
} } My source quotes M.Knoll first demonstrated the SEM in 1935 but no
} specific
} } date.
} }
} } Harry Ekstrom
} } Materials Laboratory
} }
} } (602) 231-2744
} } e-mail: harry.ekstrom-at-honeywell.com
} }
} }
} } -----Original Message-----
} } } From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
} } [mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
} } Sent: Tuesday, December 19, 2000 4:36 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Electron microscope first demonstration
} }
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Can anyone tell me the exact date in the "30's when the electron
} microscope
} } was first demonstrated?
} }
} } geinfam-at-aol.com
} }
}
}


From daemon Wed Dec 20 10:42:33 2000



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Wed, 20 Dec 2000 08:36:51 -0800
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
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I could not find an exact date ether but Burton and Kohl "The Electron Microscope," Reinhold publishing corp., 1942 in a chapter on History of the Electron Microscope says the following (p149) " In 1932 both types of electron microscopes were described and the first images were shown. E. Bruche and H. Hohannson produced the first electron images of an oxide cathode with an aperture lens system utilizing 300-volt electron beams. M. Knoll and E. Ruska developed the first magnetic electron microscope utilizing 60,000-volt electron beams. The theory of electron lenses was largely developed by J. Picht, O. Shcherzer and W. Henneberg." In the Acknowledgments at the start of the book Dr. Kohl (one of the authors) is said to have given a demonstration of the oxide cathodes experiments: "Such an electron microscope was demonstrated before the seminar of the McLennan Laboratory in April, 1934; this probably was the first such demonstration in Canada." according to Burton. This puts the non-scanning types back a few years before the SEM date given below. Burton and Kohl don't provide any better dates than these, that I could find. Hope this might help you in your search.
Jim.

James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } "Ekstrom, Harry" {harry.ekstrom-at-honeywell.com} 12/19/00 07:56PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
date.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 4:36 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com






From daemon Wed Dec 20 11:31:27 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 20 Dec 2000 12:31:34 -0400
Subject: RE: EQUIP Disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are several companies that deal in used and rebuilt scientific equipment:

1. Capovani ABrothers Inc. Tel: 518-346-8347, http://www.capovani.com

2. E. McGrath Inc. Tel: 508-744-3546
35 Osborne St, Salem, MA 01970-2599

3. Scientific Exchange Tel: 613-692-1871
P.O. Box 1441, Ogdenssburg, NY 13669

4. National Sales Equipment 800-545-0540
83 Eastman St, Easton, MA 02334

5. International Equipment Trading 708-913-0777
960 Woodlands Pkwy., Vernon Hills, IL 60061
You might try one of these (some addresses and phone numbers may be out of
date), or ytou can probably find other companies that deal with used
equipment on the Internet.

Good luckn and Happy Holidays

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Wed Dec 20 12:03:47 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 20 Dec 2000 12:59:55 -0500
Subject: Re: About tem specimen

Contents Retrieved from Microscopy Listserver Archives
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Can anybody tell me how to prepare TEM samples for Single crystal?
Thanks for your interested

Dear Erdem,
It depends on the crystal and other parameters. Are you doing
selected-area (SAED), convergent beam (CBED), or reflection (RHEED) diffraction?
Is your specimen radiation resistant--such as a mineral--or labile--such as an
organic? Will it stay solid in the vacuum, or will it need to be kept at LN2
temperature (as does anthracene)? What do you need to measure, intensities or
lattice constants? What voltage will you use? The preparation can be very
simple--e.g., MgO, which is made by burning Mg and collecting the smoke on a
formvar-coated grid--or complicated--e.g., growing copper
perchlorophthalocyanine epitaxially on freshly-cleaved alkali halide from a
vapor or isolating a protein and finding crystallization conditions that produce
well-ordered platy single crystals. Let me know some details, and I'll see if I
can help.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Wed Dec 20 12:04:30 2000



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Wed, 20 Dec 2000 18:00:58 -0000
Subject: LN2 funnels, etc

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,

Many thanks for the awful warnings, confessions and suggestions. We use two
ordinary 9cm plastic funnels which have cracked and one 20cm funnel labelled
KENUTUF, supplied with the mic to top up the Xray analysis dewar, which
still seems as good as new after at least six years.

I love the Origami solution but to replace the little funnels I'll go with
the stainless type for durability and add an insulated handle.

On the bung popping dewar front, the answer is not tin hats and a shout of
'incoming' but to provide a small vent. Even pressurised dewars need a
relief valve.

ttfn, Chris


From daemon Wed Dec 20 12:24:51 2000



From: Clow, Patricia :      Patricia.Clow-at-genzyme.com
Date: Wed, 20 Dec 2000 13:04:28 -0500
Subject: preparation TEM sample of coral

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Dr. Joseph L. Scott at The College of William and Mary (Williamsburg,
VA)...
jlscot-at-facstaff.wm.edu
His research includes TEM of coralline algae.

Patty Clow

~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Patricia A. Clow, Ph.D.
Staff Scientist I / Microscopy
Genzyme Corporation
One Mountain Road
PO Box 9322
Framingham, MA 01701-9322
phone: 508-270-2053
fax: 508-872-9080
email: patricia.clow-at-genzyme.com



-----Original Message-----
} From: Feng Wu [mailto:fwu-at-gbumail.bgu.ac.il]
Sent: Wednesday, December 20, 2000 3:35 AM
To: Microscopy-at-sparc5.microscopy.com


Can anybody tell me how to prepare TEM samples for biomaterials such as
coral?
Thanks.
Feng
**************************************************
Dr. Feng Wu
Dept. of Materials Engineering
Ben-Gurion University of the Negev
Beer-Sheva 84105, Israel

************************************************



From daemon Wed Dec 20 12:32:39 2000



From: NPGSlithography-at-aol.com
Date: Wed, 20 Dec 2000 13:29:27 EST
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

The current discussion of user fees appears to have strong, contrary
opinions, so it seems worthwhile to take a look at what is posted at the US
Department of Justice web site.

The following is taken "http://www.usdoj.gov/atr/foia/divisionmanual/ch2.htm".

} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } { { { { { { {
1. SHERMAN ANTITRUST ACT, 15 U.S.C. §§ 1-7

§ 1 Sherman Act, 15 U.S.C. § 1

Trusts, etc., in restraint of trade illegal; penalty

Every contract, combination in the form of trust or otherwise, or conspiracy,
in restraint of trade or commerce among the several States, or with foreign
nations, is declared to be illegal. Every person who shall make any contract
or engage in any combination or conspiracy hereby declared to be illegal
shall be deemed guilty of a felony, and, on conviction thereof, shall be
punished by fine not exceeding $10,000,000 if a corporation, or, if any other
person, $350,000, or by imprisonment not exceeding three years, or by both
said punishments, in the discretion of the court.

§ 2 Sherman Act, 15 U.S.C. § 2

Monopolizing trade a felony; penalty

Every person who shall monopolize, or attempt to monopolize, or combine or
conspire with any other person or persons, to monopolize any part of the
trade or commerce among the several States, or with foreign nations, shall be
deemed guilty of a felony, and, on conviction thereof, shall be punished by
fine not exceeding $10,000,000 if a corporation, or, if any other person,
$350,000, or by imprisonment not exceeding three years, or by both said
punishments, in the discretion of the court.

(There are many more sections, but I believe these two summarize the main
issues of the Act.)
{ { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {

Based on a common sense reading of the above, it seems to me that "restraint
of trade" and the "attempt to monopolize" are the key factors that that
Sherman Antitrust Act is designed to prevent, however, the issue of "intent"
is not obvious.

I would think that a discussion by EM facility managers about how they charge
for the use of their facilities and services, where the main emphasis seems
to be how to cover the expenses of running the facilities, does not appear to
be an intentional "restraint of trade" or an "attempt to monopolize" their
trade.

I would agree that any suggestion of price fixing or how to undercut the
rates of private companies would certainly violate the Sherman Antitrust Act,
but I hardly think anyone can argue that any such comments have been made in
the current discussion.

However, the question remains that if the well intentioned discussions
between managers of nonprofit and/or university supported facilities on how
to charge to cover their operating costs results in wide spread rates that
actually do undercut private companies, is that illegal? I will refrain from
presenting my opinion, since I believe that only someone with the appropriate
legal background can give an authoritative answer to this question. And
considering that every lawsuit has lawyers representing the opposing sides,
the answer may only be clear if there is already a precedent on record where
a similar case has actually gone through the courts.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Wed Dec 20 12:41:54 2000



From: Jo Ann Moore :      jamoore-at-hsc.usf.edu
Date: Wed, 20 Dec 2000 13:46:16 -0500
Subject: Aus Jena condenser

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists,

We are looking for a condenser for an old light microscope, an Aus Jena
brand. Does anyone know where we might find such an item?

Thanks, Jo Ann Moore
University of South Florida
Anatomy Department



From daemon Wed Dec 20 14:45:54 2000



From: JHumenansky-at-phi.com
Date: Wed, 20 Dec 2000 14:39:24 -0600
Subject: Re: Reposting/ User Fee's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Personnel salaries ARE part of the operating costs of running any type of
laboratory facility. In addition, the cost of an electron microscope is
also part of the operating expense of operating a lab. To suggest that
only the non personnel costs such as service contracts, supplies etc.
should be counted as operating costs really distorts the true picture.

I came from a university lab that originally was small and able to meet all
of its operating expenses including salaries. Eventually visionaries saw
the need for a larger more grand facility that would include some
instruments with an annual service contract around 35K, other contract
costs were in the range of 9K to 20K per year. The facility was built and
the rates were kept low for academic users (about $25/hr.) and rates for
non university users were scaled depending upon association with the
facility. It didn't matter that it required about 1400 hours of beam time
on one instrument just to pay the service contract because the facility
received 8-10 million dollars per year in support from NSF. Principle
instruments were obtained in grants and other subsidies not related to
operating expenses. Eventually the NSF contract expired and now with aging
equipment technology is passing them by.

The point of this is that nearly all university facilities will eventually
have to be accountable for all of their expenses and adjust their rates to
cover the costs. But universities still will have an advantage in that
they usually don't have to purchase the principle equipment from operating
expenses. How NSF granted instrumentation can be used is described in NSF
Important Rule 91.

When university labs have to account for all of their operating expenses,
they will become competitors of private non-subsidized labs. If they offer
instruments rates less than private lab, they are cheating.

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387



From daemon Wed Dec 20 15:13:38 2000



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Wed, 20 Dec 2000 16:08:19 -0500
Subject: lab design book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I get a rare opportunity to renovate some space to move our EM suite and have to turn in the ideal lab concept very soon. I know there is a book on this very thing. Anyone recall the title and author??



From daemon Wed Dec 20 15:19:50 2000



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Wed, 20 Dec 2000 16:15:13 -0500
Subject: ESEM users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am now in charge of a Phillips XL30 ESEM and have a couple of questions. What are you doing to prevent contamination of the specimen chamber. I had a user wanting to view human tissue today unfixed and am reluctant to do so. Anyone have a solution. Mine was to fix and process for standard SEM but that may not be an option for some of the in-situ studies or preserved museum specimens they don't want to wash or damage. Thanks



From daemon Wed Dec 20 15:19:51 2000



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Wed, 20 Dec 2000 16:17:44 -0500
Subject: Re: LN2 funnels, etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Small stainless steel funnels are used to fill many LN2
} anticontamination devices on electron microscopes. I have found that a
} Java jacket (designed for holding hot coffee cups) makes a great
} reuseable jacket for picking up these funnels when they are cold.

John Hunt
CCMR Microscopy Facility
255-0108



From daemon Wed Dec 20 15:39:19 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 21 Dec 2000 10:41:22 GMT+1200
Subject: video camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi again

I want to cheaply mount an inexpensive video camera onto the optical
microscope of, yes, you guessed it, a JEOL 840. Economically, you
might say.

Somebody been there, or near there?

I'm not very knowledgeable about optics, do I need to get

1 a lensless video camera and take off the microscope eyepiece,

2 a lensless video camera and retain the microcope eyepiece,

3 a video camera with a lens and take off the microscope eyepiece,

4 a video camera with a lens and retain the microscope eyepiece,

or

5 none of the above?


I'm sure I can devise the neccessary spacer/adaptor

tia

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed Dec 20 16:00:29 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 20 Dec 2000 15:53:54 -0600
Subject: lab design book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

One volume is "Design of the Electron Microscope Laboratory", by Ronald
Alderson. It is part of the Glauert series, "Practical Methods in Electron
Microscopy". The edition we have is from 1975 and I don't know if there is
a newer one.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Scott Whittaker [mailto:Whittaker.scott-at-nmnh.si.edu]
Sent: Wednesday, December 20, 2000 3:08 PM
To: Microscopy-at-sparc5.microscopy.com


I get a rare opportunity to renovate some space to move our EM suite and
have to turn in the ideal lab concept very soon. I know there is a book on
this very thing. Anyone recall the title and author??



From daemon Wed Dec 20 16:31:48 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Wed, 20 Dec 2000 12:50:08 -0700
Subject: RE: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well Kids,

I think the point we are missing here is geinfam-at-aol.com asked when the
first EM microscope was first "demonstrated". My source states the
theoretical "description" of the SEM was brought to us by H. Stintzing in
1929. However the first "demonstration" of an SEM was in 1935 by M. Knoll.
In 1932, the first TEM was "constructed" by Knoll and Ruska. I wonder how
they all felt about the electoral college.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com




From daemon Wed Dec 20 16:46:09 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 21 Dec 2000 00:34:02 -0000
Subject: Re: Reposting/ User Fee's

Contents Retrieved from Microscopy Listserver Archives
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Go to this site for some interesting info.

http://ernst.ruska.de/daten_e/mainframe_e.html

Barry
EMU
UNSW


----- Original Message -----
} From: Fortner, Jeffrey A. {fortner-at-cmt.anl.gov}
To: 'Microscopy Listserver' {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, December 21, 2000 3:32 AM


I worked out the real, full-costs-recovery cost of our emlab a few
years ago, and came to the conclusion that we would have to
charge around £1250 a day to our users for access to a TEM. The
charge for basic access to a top-grade analytical FEG TEM at a
commercial rate could easily be in the order of £3000 per day. The
task for most of us, as you indicate, is to keep the running costs
down to a level which the University can tolerate. Most University
facilities I know of in the UK are free of the requirement to earn the
replacement cost of the equipment, and have to earn only limited
staff costs. I am aware that there is ever-increasing pressure on
Universities to earn more of the costs of their operations. The latest
rumour is that electricity and water charges are just around the
corner, but that one is at least a decade old now. I'll believe it when
they install the meters. Maybe it'll come, maybe it won't. But in the
past 15 years of operating a facility I swear that the user attitudes to
charging don't reflect any recognition of this. Internal users still
lobby for access to be free, or at least to appear free at the point of
use. We therefore make just a few k per annum towards our costs
by selling a little slack time to any local industries that have a need
for our services. Who exactly are we cheating by doing this? An
alternative way of looking at it is that we are offering the public some
access to a facility they paid for in the first place, at a rate which is
no more than is necessary to keep the machines running. I
personally don't know of any commercial operation anywhere within
hundreds of miles of Edinburgh that we are in competition with.
Our operation is strictly self-limiting. once we have made the few k
required to cover our costs the focus is back on internal user
support. If there is any real industrial EM business out there any
competent commercial operation would have no difficulty whatever
in surviving whatever level of pseudo-commercial activity Universities
are involved in.
If there is any real evidence to the contrary I would like to hear it
(and would be amazed)
We're no threat, so please don't keep shooting us!
Chris


} The point of this is that nearly all university facilities will eventually

} have to be accountable for all of their expenses and adjust their rates to
} cover the costs. But universities still will have an advantage in that
} they usually don't have to purchase the principle equipment from operating
} expenses. How NSF granted instrumentation can be used is described in NSF
} Important Rule 91.
}
} When university labs have to account for all of their operating expenses,
} they will become competitors of private non-subsidized labs. If they offer
} instruments rates less than private lab, they are cheating.
}
} John Humenansky/Staff Scientist
} Physical Electronics, Inc. (PHI)
} 6509 Flying Cloud Drive
} Eden Prairie, MN 55344
} 952-828-6387
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Wed Dec 20 19:40:24 2000



From: =?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?= :      jdyun-at-kyungnam.ac.kr
Date: Thu, 21 Dec 2000 10:23:04 +0900
Subject: Thickness measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

Can anybody suggest how to measure thickness of the conductive coating for EM specimen? If it is transparent, ellipsometer could be used. Since metallic or carbon coating is not transparent, I could not use that method. Then do I have only way to measure directly, like measuring thickness on the cross-section or on the surface by using EM or SPM? Is there any other way?

Jondo Yun
jdyun-at-kyungnam.ac.kr


From daemon Wed Dec 20 20:37:38 2000



From: Joon Hwan CHOI :      jchoi-at-engineering.ucsb.edu
Date: Wed, 20 Dec 2000 18:32:22 -0800
Subject: TEM specimen preparation of titania particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Collegues,

I have the following problem during my TEM observation.
I am looking at titania (TiO2), rutile, particles which are placed on top of a carbon coated cooper grid. Those particles are ca. 90 nm long and ca. 15 nm wide. At 200 kV the particles are very unstable under the electron beam. I think charging occurs. The attempt to coat the sample with carbon did show some success, but even after very long sputtering times (15 min. at 25 A) most particles twinkle.

If anybody has an idea for me how to avoid the twinkling, preferentially without sputtering any carbon on top of the sample, I would be very thankful.

Sincerely,
Joon

************************************************************
Joon Hwan CHOI, Ph.D.
Post-doctoral Researcher
Materials Department
University of California at Santa Barbara (UCSB)
Santa Barbara, CA 93106

Mailto: jchoi-at-engineering.ucsb.edu / joonhwanchoi-at-netzero.net

Office Phone (805) 893-2008 / Fax (805) 893-8971, 8486
************************************************************


From daemon Wed Dec 20 21:09:37 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 21 Dec 2000 01:52:04 -0500
Subject: RE: TEM specimen preparation of titania particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Ernst Ruska received in 1986 the Nobel Prize in Physics "for his fundamental
work on electron optics and for the design of the first electon microscope".

You can read more on the Official Web Site of the Nobel Foundation:
http://www.nobel.se/physics/laureates/1986/index.html

Cheers,

Paul
======================
Paul Baggethun
Alcoa Technical Center
Alcoa Center, PA 15069
USA
======================

-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 6:36 PM
To: Microscopy-at-sparc5.microscopy.com


I think the twinkling problem is more electron beam heating of your sample as opposed to charging. If the samples were charging, they would be moving on the carbon support film. Carbon coating the samples will help. Are you using holey carbon grids or continuous support films. The latter should help somewhat, but you have about 200A of carbon that adds to the thickness. You could also try a cold stage to see if that could help. You could also try a higher voltage machine if one is available to you. Higher voltages allow the electrons to pass through your sample with less interaction and therefore less heating occurs.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Joon Hwan CHOI [mailto:jchoi-at-engineering.ucsb.edu]
Sent: Wednesday, December 20, 2000 9:32 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: TEM specimen preparation of titania particles


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Dear Collegues,

I have the following problem during my TEM observation.
I am looking at titania (TiO2), rutile, particles which are
placed on top of a carbon coated cooper grid. Those particles
are ca. 90 nm long and ca. 15 nm wide. At 200 kV the particles
are very unstable under the electron beam. I think charging
occurs. The attempt to coat the sample with carbon did show
some success, but even after very long sputtering times (15
min. at 25 A) most particles twinkle.

If anybody has an idea for me how to avoid the twinkling,
preferentially without sputtering any carbon on top of the
sample, I would be very thankful.

Sincerely,
Joon

************************************************************
Joon Hwan CHOI, Ph.D.
Post-doctoral Researcher
Materials Department
University of California at Santa Barbara (UCSB)
Santa Barbara, CA 93106

Mailto: jchoi-at-engineering.ucsb.edu / joonhwanchoi-at-netzero.net

Office Phone (805) 893-2008 / Fax (805) 893-8971, 8486
************************************************************



From daemon Thu Dec 21 02:39:12 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 21 Dec 2000 09:33:56 +0100
Subject: early SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


About the firts TEM, the question is answered. But about the SEM, a few
precisions.

Some interresting papers gives the essential informations about its
history. But we must keep in mind that the early SEM was a "reflexion
microscope" designed in analogy with the optical microscope. And the
development were made in paralell with the TEM. So the design looks like a
modified TEM, and not like what we know today as a SEM. It was also the
case with the first Stereoscan.

The papers :

M. Von Ardenne
Zur Geschichte der Rasterelektronenmikroskopie und der
Elektronenmikrosonde.
Optik 50 (1978) No 3, 177-188

C.W. Oatley
The early history of the scanning electron microscope
J. Appl. Phys. 53(2) Feb 1982

The first papers of Von Ardenne were from 1937 and 39 (Zeitschrift fur
Physik), and he had built an instrument and published some photography.
The intrument was destroyed by the bombardement at the end of WW II.

An other interresting paper is :

V.K. Zworykin, J. Hillier and R.L.Snder
A Scanning Electron Microscope
ASTM bulletin August 1942

After a lot of nice experiments (with FE guns!) they conclued that it was
too expensive, with a poor signal and to less resolution.


J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Thu Dec 21 06:28:47 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 21 Dec 2000 12:22:22 +0000 (GMT Standard Time)
Subject: Re: ESEM users

Contents Retrieved from Microscopy Listserver Archives
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We fix human cells for safety reasons but sometimes look at
them wet (hard work!). Chris Gilpin argues that fixation
helps specimens resist beam damage and that most distortion
occurs during drying rather than fixation.

Dave




On Wed, 20 Dec 2000 16:15:13 -0500 Scott Whittaker
{Whittaker.scott-at-nmnh.si.edu} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I am now in charge of a Phillips XL30 ESEM and have a couple of questions. What are you doing to prevent contamination of the specimen chamber. I had a user wanting to view human tissue today unfixed and am reluctant to do so. Anyone have a solution. Mine was to fix and process for standard SEM but that may not be an option for some of the in-situ studies or preserved museum specimens they don't want to wash or damage. Thanks
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Dec 21 08:38:36 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 21 Dec 2000 09:35:40 -0500
Subject: Re: lab design book

Contents Retrieved from Microscopy Listserver Archives
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At 4:08 PM -0500 12/20/00, Scott Whittaker wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*******************
Hi Scott,
I just pulled the volume off my shelf. It is part of the series
edited by Audrey Glauert: Practical Mehods in Electron Microscopy
Volume 4 "Design of the Electron Microscope Laboratory" written by
Ronald H. Alderson original copyright 1975. There may be a more
recent edition, but this one certainly covers the main considerations.

I envy your opportunity to "start from scratch" many of us end up
retrofitting spaces designed for other purposes.Have fun.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Dec 21 08:59:00 2000



From: George Langford :      amenex-at-amenex.com
Date: Thu, 21 Dec 2000 10:01:38 -0500
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
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Hello Microscopists !

By analogy, any combination (even among nonprofits) for the purposes
of setting rates must be illegal if the well publicized divvying up
of applicants to graduate school was an illegal restraint of trade.

Private for-profit industry was not involved in that particular
conspiracy in any way.

On the other hand, the mere listing of categories of expense and
the discussion of how best to allocate those costs between users
and providers seem appropriate. That's essentially the same as
a discussion of the principles of accounting or the geometry
of the Ewald sphere.

When specific rates are mentioned, that's what looks like price
fixing.

Best regards,
George Langford, Sc.D.
amenex-at-amenex.com


From daemon Thu Dec 21 09:12:53 2000



From: Valerie Woodward :      WOODWARD-at-brk.bfg.com
Date: Thu, 21 Dec 2000 10:04:06 -0500
Subject: fluorescence euipment needed

Contents Retrieved from Microscopy Listserver Archives
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Greetings all:

I am looking for a pol nosepiece for a Leitz Ortholux 2 microscope. I welcome any responses, off-list please, to the e-mail listed below.

Thanks,


Valerie P. Woodward
Senior R&D Chemist
BFGoodrich Performance Materials
Measurement Science, D/2197
Microscopy and X-ray Analysis
9921 Brecksville Rd.
Brecksville OH 44141-3289
(216) 447-5408 (voice)
(216) 447-5575 (FAX)
woodward-at-brk.bfg.com (e-mail)



From daemon Thu Dec 21 10:15:34 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 21 Dec 2000 09:53:28 -0600
Subject: user fees

Contents Retrieved from Microscopy Listserver Archives
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I disagree that posting rates implies core facilities are attempting
price-fixing. Surely commercial entities look at the published costs
of their competitors all the time. When one airline drops its rates,
the others usually follow within 24 hrs. Price-fixing only results
if you make your decisions in concert with your competitors. Knowing
how much other core facilities charge has several benefits. First,
the core knows what level of fees allow investigators to afford to
use a technology. If I charged enough to fully reimburse the true
expenses of my core, most of my researchers would simply switch to
alternative techniques since they have limited grant dollars.
Secondly, it allows core directors to demonstrate to administrators
what portion of operating expenses other cores attempt to recover
from user fees. I always tell my administrators that I am to make a
profit that rivals our university library! Lots of cores say they try
to recover operating expenses but unlike industry, we never account
for building costs, electricity and heat, maintenance, etc.
Researchers pay for those types of things with their indirect costs
that accompany virtually all grants. This makes a comparison of
commercial vs university fees impossible to judge. Indirect costs
also help many universities pay for the "matching portion" that most
federal grant agencies require as a part of matching equipment
grants. Whether I use the flow cytometer on my campus or not, my
indirect costs help pay for it. In addition, as a tax-paying
citizen of the state of Missouri, my tax dollars help subsidize our
University. We pay these taxes, in part, because we want our
students to have access to the cutting edge technologies that will
make them competitive citizens. What good would having all these fun
toys be if they were too expensive to allow our undergrads and grads
some time on them. These subsidies have no equivalent with private
industry.

Anyone wanting to see what MU charges for confocal and other types of
LM, should surf over to
http://www.biotech.missouri.edu/mcc/prices.html . The costs for our
EM core are at http://www.biotech.missouri.edu/mbp/cores/ . I am
responsible for posting the LM core prices but Randy Tindall is
responsible for the EM core prices being posted so please direct all
lawsuits regarding them at him (Sorry, Randy). See you in court.

Merry Christmas and Happy Holidays to all. Tom




--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu Dec 21 10:19:31 2000



From: Brian W Robertson :      brobertson-at-unl.edu
Date: Thu, 21 Dec 2000 10:16:07 -0600
Subject: lab space

Contents Retrieved from Microscopy Listserver Archives
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Dear Scott
I had the same opportunity to design for an EM lab when I moved to the
University of Nebraska -- new facility, new space. Fortunately the lab
spaces offered included one that I checked to be virtually ideal. This was
in the basement of a building that had 3 floors above ground plus the
basement and that was intended to be suitable for adding another 3 floors
on top. The footings and the (1 foot thick reinforced concrete) wall
surrounding the lab were really massive and confer the best stability I've
heard of -- floor vibration velocities { 0.5micrometers per second (at
frequencies 1 to } 50 Hz) and amplitudes { 0.5 micrometers (peak to peak)
and AC magnetic fields {0.5 mG (before EM equipment installation). I got
the architectural and construction team to provide several important
provisions for use of the space for EM:
1. laminar air inlets to rooms (positioned so as not to affect the TEM
columns!),
2. near-floor level return air vent in the TEM rooms (to satisfy needs to
remove SF6 since it is denser than air),
3. acoustic lining of air inlet ducts,
4. temperature regulation to +/- 1 C with a maximum rate 1 C / hour,
5. humidity regulation in the range 40 to 60% relative humidity year round
6. water chillers in a mechanical facilities room adjacent to, but outside,
the EM facility.

The above aspects overlap and go beyond the necessarily somewhat outdated
recommendations in Anderson's book.

There were still issues that required care in order for the TEM to meet the
image drift specifications I required of the manufacturers. These measures
included adjustment of the air flow in the HREM room and reduction of the
temperature difference between the TEM water temperature and the room air
temperature. The TEM water chiller also required to be equipped with the
precision electronic temperature regulation option. Under these conditions,
there is no need to install curtains or otherwise to curtail air
circulation during high resolution work.

I am very happy that our facilities design and management staff,
construction and maintenance people, and microscope manufacturer's
representatives could all be persuaded to achieve the desired end result --
continued, essentially trouble-free, stable operations of our lab in all
conditions of weather and use of the rest of the building. It is definitely
worth taking great care over the major and minor details before you start!

If I can provide other information. please let me know.
Best wishes
Brian Robertson

***********************************************************
Assoc. Prof. Brian W. Robertson
Department of Mechanical Engineering
and Center for Materials Research and Analysis
University of Nebraska-Lincoln, N124 WSEC,
17th & Vine Sts., Lincoln, NE 68588-0656, USA
** Office 402 472 8308; FAX 472 1465; Labs 472 8762 and 472 5498 **


From daemon Thu Dec 21 10:24:22 2000



From: Bruce Brinson :      brinson-at-rice.edu
Date: Thu, 21 Dec 2000 10:22:46 -0600
Subject: Re: video camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI Ritchie,
here is my cheap fix ( {1K$US)
As you mention in 1-4 the eye piece is the trick. If you have std 23 mm
eye pieces (For larger slip in eye pieces you can make a bushing) you can
buy a relay lens from Edmund Scientific & others (23 mm x c-mount). Then buy
a color security camera w/c-mount & connect to your video recording device.
I use a Snappy TM interface to my laptop. I can throw all this in my laptop
tote sack & aquire pretty decent images at almost any optical u-scope I walk
up to.
ball park on economics, optics $500, camera $200-300, Snappy {$100US
For higher res. images there has been a lot of buzz on this list server
about interfacing the Cool Pic TM digital camera. ck the archives.
Don't forget to recalibrate you magnification.

If you have thread on eye pieces... I'd like to know what you find out.

disclaimer: no financial interest in companies or products mentioned.

Happy holidays,
Bruce Brinson
Rice U.

Ritchie Sims wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Hi again
}
} I want to cheaply mount an inexpensive video camera onto the optical
} microscope of, yes, you guessed it, a JEOL 840. Economically, you
} might say.
}
} Somebody been there, or near there?
}
} I'm not very knowledgeable about optics, do I need to get
}
} 1 a lensless video camera and take off the microscope eyepiece,
}
} 2 a lensless video camera and retain the microcope eyepiece,
}
} 3 a video camera with a lens and take off the microscope eyepiece,
}
} 4 a video camera with a lens and retain the microscope eyepiece,
}
} or
}
} 5 none of the above?
}
} I'm sure I can devise the neccessary spacer/adaptor
}
} tia
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand



From daemon Thu Dec 21 10:49:26 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 21 Dec 2000 10:38:45 -0600
Subject: Rates

Contents Retrieved from Microscopy Listserver Archives
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Tell you what, folks. Next time we marauding academic pirates are in need
of guidance on fees, let's just ask for links to web pages where other labs
already have their rates published. Maybe that will make everybody happy
and serve the same purpose as our usual blatant conspiracy does. That way
we can take over the whole world legally!

Yeah, that's the ticket.....

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Dec 21 10:51:12 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 21 Dec 2000 17:47:24 +0100
Subject: LaB6 filament problem

Contents Retrieved from Microscopy Listserver Archives
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Hello

Has some a recept to clean the ceramic holder from a LaB6 filament. It is
shorted (300 kohm at rohm temp., 0 at working temp.) with the filament.
The tip is OK. Is it a way to remove the evaported coating (LaB6,
decomposed LaB6 or carbon ?) on the ceramic, without dammaging the tip.
Mechanically seems to bee inpracticable, it's too small (10 mm diameter, 5
mm heigth between the ceramic holder and the graphite blocs), and I have
no "microbillage" (I don't know in english). But chemicaly ? I don't know
the manufacturer of the tip, it comes from a LEED optic.

Thanks

J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Thu Dec 21 10:52:53 2000



From: JHumenansky-at-phi.com
Date: Thu, 21 Dec 2000 10:49:05 -0600
Subject: Re: User Fee's

Contents Retrieved from Microscopy Listserver Archives
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Your reply proves my point. If you were a business your personnel salaries
and instrument costs would have to be factored into the ACTUAL operating
costs. The instrument rates that you charge for outside clients are based
on YOUR operating costs which do not include salaries or instruments and
can therefore be much lower than what a business MUST charge to recover
their costs and make a profit. Many universities are not in competition to
private business because they are funded internally and exist primarily for
education and research. Too often however the economics of operating a
university facility collide with the reduction in institutional funding and
then outside users are sought which is the unfair competition aspect unless
the university rates for outside users are the same as what the businesses
are charging for similar equipment.

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387



From daemon Thu Dec 21 11:49:40 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Thu, 21 Dec 2000 12:46:27 -0500
Subject: Re: user fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom Phillips wrote:

} We pay these taxes, in part, because we want our
} students to have access to the cutting edge technologies that will
} make them competitive citizens. What good would having all these fun
} toys be if they were too expensive to allow our undergrads and grads
} some time on them.

I would also argue that students who never have a chance to see or use an
electron microscope during their training are less likely to seek out those
services later when they go to work in the private sector. I strongly
suspect that the availability of EM in Universities is an important factor
in creating a market for private sector EM providers.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Thu Dec 21 14:09:26 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Thu, 21 Dec 2000 15:05:46 -0500
Subject: Fwd: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
}
} One point that seems to have been missed is that the various
} university labs do not compete with each other but rather need to recover
} costs within their institution. This is a cost recovery and
} redistribution process from within a single fiscal entity. (All grants
} etc. are university funds and no longer belong to the granting agency or
} the PI.) So I don't get this restraint of trade nonsense. We do not
} sell anything within our institution. We provide a service that must be
} financially supported in one way or another and it is most appropriate to
} have the users of our facility provide all or part of those costs. So I
} figure we can discuss anything we damn well please among professionals
} who provide these services.
}
} At 10:01 AM 12/21/2000 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251


From daemon Thu Dec 21 18:05:52 2000



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 21 Dec 2000 17:59:10 -0600
Subject: Re: Administrivia: User Fee's

Contents Retrieved from Microscopy Listserver Archives
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Colleagues....

I think all opinions have been aired and nothing new
has been said for awhile. I'm going to suggest that the
thread be left to ferment for another year when I'm sure
the question will get asked once again.

Nestor
Your Friendly Neighborhood SysOp







From daemon Thu Dec 21 18:37:38 2000



From: John F. Turner :      j.f.turner-at-csuohio.edu
Date: Thu, 21 Dec 2000 19:38:03 -0600
Subject: Re: video camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ritch,

It's a good bet that it will work. If it doesn't, try changing the
distance between the microscope and the camera. Also, if you cannot
fill the video chip completely with the image, or if it is over filled
or distorted, try using the video lens with an eyepiece preceding it
before you consider purchasing any additional optics. The alignment of
the eyepiece and video lens from side to side, as well as the distance
separation them are critical. A small deviation can lead to large
changes in the image appearance and quality. There is a sweet spot, so
patience prevails. Again, this is not the optimal way to interface a
video camera and a microscope, but it is cost effective and performs
well in many non-critical applications. If the video lens has an
adjustable focus, it may be possible to use it without the eyepiece, and
it is worth a try if all else fails.

Best of luck

John


John Turner, Ph.D.
Director, Advanced Chemical Imaging Facility
Cleveland State University
Cleveland, Ohio 44115




From daemon Thu Dec 21 21:37:09 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 21 Dec 2000 23:26:32 -0800
Subject: Re: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I beleive the first high resolution SEM was built by Oliver Wells as a PhD project
in 1959. It was enabled by the Everhart-Thornley secondary electron detector. My
understanding is that the theory of how an SEM should work was developed in the
'30s but specimen current and other available signals couldn't be amplified
sufficiently or cleanly enough to be useable. Most of them still can't be used for
high resolution work.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Jim at ProSciTech wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} In 1935? That was a TEM, not an SEM. Knoll was Ernst Ruska's PhD supervisor and
} later collaborator. They designed and built the first TEMs.
} The very first model had no specimen chamber and just proved that the lens
} system could work. So the first model, which I seem to remember was before 1935
} was really not a microscope.
} Von Ardenne described the operating principle of an SEM in the late 30th, but I
} believe none was build until the early 60th at Cambridge University, UK.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, December 20, 2000 1:57 PM, Ekstrom, Harry
} [SMTP:harry.ekstrom-at-honeywell.com] wrote:
} }
} }
} } My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
} } date.
} }
} } Harry Ekstrom
} } Materials Laboratory
} }
} } (602) 231-2744
} } e-mail: harry.ekstrom-at-honeywell.com
} }
} }
} } -----Original Message-----
} } } From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
} } [mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
} } Sent: Tuesday, December 19, 2000 4:36 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Electron microscope first demonstration
} }
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Can anyone tell me the exact date in the "30's when the electron microscope
} } was first demonstrated?
} }
} } geinfam-at-aol.com
} }



From daemon Fri Dec 22 08:38:36 2000



From: Jean-Claude Lebosse :      Jean-Claude.Lebosse-at-insa-lyon.fr
Date: Fri, 22 Dec 2000 15:48:39 -0500
Subject: Use of ICXANES

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listmembers,

Is there somebody who know how to calculate fine structures near L23 edges by using
ICXANES, while this code is restricted to spectra involving core levels of s symmetry,
i.e. K, L1...?

Best regards.

JC Le Bossé and R Chassagnon


From daemon Fri Dec 22 08:41:22 2000



From: Dr. Volker Brinkmann :      brinkmann-at-mpiib-berlin.mpg.de
Date: Fri, 22 Dec 2000 15:38:34 +0100
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






From daemon Fri Dec 22 09:08:48 2000



From: Zhenquan Liu :      zhenquan.liu-at-asu.edu
Date: Fri, 22 Dec 2000 08:04:45 -0700
Subject: About tem specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Erdem,

Please tell us some details about your email. Do you have a sample to
prepare? Or you just want to know the general knowledge?

Cheers!

Zhenquan Liu

---------------------------------------------------------------------------

Dear Sir/Madam,
Can anybody tell me how to prepare TEM samples for Single crystal?
Thanks for your interested




**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************



------------------------------------
Zhenquan Liu (Dr.)
Arizona State University
CSSS, CHEM
Room PSA213,
Tempe, AZ 85287
Tel (480) 965 4544 (o)
(480) 775 7428 (h)
Fax (480) 965 9004 (o)
Email zhenquan.liu-at-asu.edu
------------------------------------




From daemon Fri Dec 22 10:40:47 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Fri, 22 Dec 2000 08:36:49 -0800
Subject: RE: SEM: mystery CL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Warren writes ...

} Are you going to fill us in on the answer, or do you even know it?
} ...
} Inquiring minds want to know.

I thought I had ... it is a mystery to me how some posts, for example
this facility supervisor vacancy's announcement, get cross-posted
everywhere, and why the subsequent discussion of this image missed
this list :o)

http://epmalab.uoregon.edu/images/zircon-xmas.jpg

It is an image of e-beam induced CL as acquired via RGB filters. The
blue is somewhat typical of igneous (or primary) quartz ... the red
halo is evidence of radiation damage from a nearby zircon inclusion.
Other images from the same rock actually show us the "smoking gun".
We investigated a number of sandstones, and otherwize quartz bearing
rocks from this environment, and another type of radiation induced CL
we observed was red indistinct rims on qtz grains in sandstone. We
imagined radioactive fluids percolating through the rocks.


happy holidays ... shAf:o)



From daemon Fri Dec 22 11:17:32 2000



From: Ingber, Bruce F. :      bingber-at-commserver.srrc.usda.gov
Date: Fri, 22 Dec 2000 11:18:34 -0600
Subject: RE: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
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My turn for True Confessions or surviving liquid nitrogen incidents.

Many years ago in another time warp (70's) a "green" technician (no, not
environmental green) was dutifully following his supervisor's orders to fill
EDX detector Dewars with liquid nitrogen. The staff had used a heavy-duty
plastic Nalgene pitcher, wrapped with lab tape, to accomplish this chore.
Thus, the technician followed protocol and used the same pitcher to collect
the cryogenic fluid. And it worked well for many, many weeks. However, one
day as he was filling the wrapped container, there was an enormous explosion
(or implosion?). The technician was left holding the handle of the pitcher
with the rest of the pitcher vanished into hundreds of small plastic pieces
and liquid nitrogen splattered everywhere. Holy mackerel! But for the grace
of God, not one piece of plastic or drop of liquid nitrogen hit the
SURPRISED technician!

However, to this day that technician remembers his mother's admonitions
about wearing clean underwear, you never know when an eighteen wheeler will
hit you.

Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
(504) 286-4270 phone
(504) 286-4419 fax
bingber-at-commserver.srrc.usda.gov




From daemon Fri Dec 22 11:38:02 2000



From: =?ISO-8859-1?Q?Laboratorio_de_Microscop=EDa_-_UNER?= :      microsc-at-fi.uner.edu.ar
Date: Fri, 22 Dec 2000 13:38:39 -0000
Subject: unsuscribe

Contents Retrieved from Microscopy Listserver Archives
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===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electrónica
Facultad de Ingeniería - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================


From daemon Mon Dec 25 17:22:49 2000



From: service-at-promoteacasino.com
Date: 25 Dec 2000 19:57:31 -0500
Subject: Promote a casino

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Internet Marketer,

Are you in the bulk email/internet marketing business and want to make some serious $$$ by owning your own ONLINE CASINO!! Check out this latest HOT HOT concept at www.promoteacasino.com

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P.S. You have been contacted because your company/you were listed as being in the internet marketing business. If you don't reply back, YOU WILL NEVER BE CONTACTED AGAIN.


From daemon Tue Dec 26 07:36:47 2000



From: Beth Bray :      bbray-at-sc.rr.com
Date: Tue, 26 Dec 2000 19:49:18 -0500
Subject: RE: preparation TEM sample of coral

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all
Thanks for your attention to my question on coral TEM specimen. The coral
is hard, but not dense. A lot of platelets arranged in radiation way were
observed in optical microscope. Can anybody give me some suggestion to
prepare it?
Thanks again.
**************************************************
Dr. Feng Wu
Dept. of Materials Engineering
Ben-Gurion University of the Negev
Beer-Sheva 84105, Israel

fax 972-7-6472944
tel 972-7-6461473(o)
-6281432(h)
email: fwu-at-bgumail.bgu.ac.il
************************************************
----- Original Message -----
} From: Feng Wu {fwu-at-bgumail.bgu.ac.il}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, December 20, 2000 10:34 AM


Dear Dr. Feng Wu,

I have used the following procedure to fix specimen of Leptogorgia
virgulata, an octocoral (sea whip) found off the eastern coast of the USA.
When fixed according to this procedure, whole tissue specimen, as well as
the harvested sclerites by themselves, can be prepared for viewing in either
SEM or TEM. I hope you find this information helpful.

Regards,

Elizabeth Bray
Plant Chemist
SCE&G Central Laboratory
2102 N. Lake Dr.
Columbia, SC 29212
USA


Fixing and Embedding Protocol for Octocorals and Their Sclerites
================================================================

Reagent Preparation:
1. Purchase the following:
16% Paraformaldehyde (PFA)
50% Gluteraldehyde (GA)
NaCacodylate, reagent grade [Sodium cacodylate (CH3)2AsO2Na · 3H2O, MW =
214.02]
LR White acrylic embedding medium

2. 0.1 M NaCacodylate (CH3)2AsO2Na · 3H2O, MW = 214.02
21.4 g NaCacodylate diluted to 1000 mL with DW. Adjust the pH to 7.4 with
4% NaOH or 1:10 HCl.

3. Fixative: 2% PFA, 0.15% GA, 0.05 M NaCacodylate in filtered Sea Water:
6.3 mL of 16% PFA
0.15 mL of 50% GA
25.0 mL of 0.1 M NaCacodylate, pH = 7.4
18.55 mL of Millipore filtered Sea Water
Total Volume of fixative = 50 mL

Procedure:
1. Fix the specimen in PFA/GA/NaCaco fixative for 1 hour.
2. Rinse in 0.05 M NaCaco Buffer 3 x 10 minutes.
3. Dehydrate in a series of EtOH (ethyl alcohol) washes of ever increasing
strength:
50% EtOH 10 minutes.
75% EtOH 10 minutes.
95% EtOH 15 minutes.
100% EtOH 15 minutes x 2, done under vacuum.

4. Three (3) changes of LR White, all done under vacuum.
The first two changes for at least 30 minutes, and the last change
overnight.
The last change should be done in the BEEM capsules in which the specimen
will be cured.

NOTE: Be sure that when you fill the capsules with the LR White for the
final time, there are no air bubbles in them. This will cause the level of
embedding medium to become lower in the capsule as it sits overnight under
vacuum, and when you cap them for curing in the oven, the air inside will
lead to incomplete curing making it difficult, if not impossible, to
properly section the hard coral material. To ensure that there are no
bubbles present, allow the capsules to sit under vacuum for 30 minutes to 1
hour, go back and top off any capsules that need it, then reestablish the
vacuum and allow them to sit overnight. Before capping, ensure that the
level of embedding medium is at the top of the capsule. If it is not,
carefully add sufficient medium to restore the proper level and proceed with
capping.

5. Cap to ensure the exclusion of air and cure overnight in a 60o C oven.



From daemon Wed Dec 27 05:40:24 2000



From: Christensen, Kim :      ChristeK-at-whiteoaksemi.com
Date: Wed, 27 Dec 2000 06:31:47 -0500
Subject: TEM: Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A position as a TEM Engineer is open at Infineon Technologies, Richmond.

Experience Requirements: The candidate should have at least a BS degree and
1 or more years of TEM analysis experience. They should be able to work in a
team environment, possess good written and verbal skills and excel at
problem solving. Additionally, they should understand the fundamental tools,
techniques and failure mechanisms associated with TEM analysis of
semiconductors and be able to assist technicians in planning and performing
the analysis.

Job Responsibilities: To coordinate, optimize and perform TEM analysis in
support of DRAM fabrication.

The candidate will perform:
1: Coordination of TEM analysis jobs with lab customers and TEM
technicians
2: Compilation, documentation and presentation of analysis results.
3: Coordination of tool maintenance and development.
4: TEM sample preparation using mechanical polishing and focused ion
beam (FIB) techniques.
5: TEM inspections of CMOS semiconductor interfaces, dislocations,
films and defects.

About Infineon Technologies Richmond: Infineon Technologies' Richmond is
situated on 210 acres in the White Oak Technology Park, 14 miles east of
downtown Richmond, Virginia in the eastern part of Henrico County. Infineon
Technologies Richmond is just four miles east of the Richmond International
Airport, with easy access to major interstate highways. The Atlantic Ocean,
Washington, D.C. and the Blue Ridge Mountains are all within a two-hour
driving radius. We are a fully integrated semiconductor manufacturer using
cutting-edge equipment and technology. Our facilities include a
state-of-the-art wafer fab, probe, test, assembly and module manufacturing
operations. We were Semiconductor International Magazine's choice for "Top
Fab of the Year" in 1999. For further information on benefits and employment
opportunities, please visit our website at www.whiteoaksemi.com.

Contact:

Kim Christensen
Ph: 804 952 7307
FAX: 804 952 7902
E-mail: kim.christensen-at-infineon.com



From daemon Wed Dec 27 08:59:20 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Wed, 27 Dec 2000 09:53:58 -0500
Subject: Re: lab design book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Scott:

You may be referring to Vol. 4 of Practical Methods in Electron
Microscopy, A. Glauert, ed., North Holland Pub., 1975. The book is a
monograph by Ronald H Alderson on "Design of the Electron Microscope
Laboratory". It has a lot of good information, but may be getting a
bit dated for the ultimate level of environment for today's most
sophisticated and sensitive instruments.

hope this helps...

Larry






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Wed Dec 27 19:19:55 2000



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 27 Dec 2000 19:00:20 -0600
Subject: LR White protocol for plant tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I am looking for a protocol for embedding plant tissues in LR White resin. I
am working with endosperm and plant embryo of seeds of Ilex paraguariensis
(maté). The endosperm contains a large amount of proteins and lipids.
Thank's.

Dr. Rinaldo Pires dos Santos
Dept. of Botany - UFRGS
Brazil
E-mail: rinaldop-at-uol.com.br
Tel: + 55 51 4964053 (home)
+ 55 51 3167634 (UFRGS)




From daemon Thu Dec 28 01:09:34 2000



From: =?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?= :      jdyun-at-kyungnam.ac.kr
Date: Thu, 28 Dec 2000 15:38:21 +0900
Subject: Re: Thickness Measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sergey, Tobias and other listers:

Thank you for your kind replies.
The equipment that you mentioned seems too expensive for me to use, especially when I need just a couple of times thickness measurement for the calibration of my sputter coater.
Is there other way to measure the thickness? Tobias suggested to use ellipsometry technique because the metallic or carbon coating transmits light when it is very thin. Have any you ever tried this before?

Best wishes,

Jondo Yun
Kyungnam University, Korea
jdyun-at-hanma.kyungnam.ac.kr

====================================================================
Hello Jondo

It looks like nobody answer your question.

There are special machines called "thickness monitor" on the market. You
have to go to your preferable EM supplier and ask them about that. It's
very usual piece of equipment. It measures directly the thickness of the
deposited material on the sensor. Sensor is mounted inside the vacuum
chamber close to your sample. It is pretty sensitive (down to the 0.1
nm). The cost - couple of thousand backs. If you have further questions -
I'll be happy to help you.

Have a good holiday season.

Sergey.

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu

===============================================================
Jondo,
Keep in mind that unless you deposit a really thick coating,
carbon or metal coats do transmit light so ellipsometry could work. I
have no personal experience with this, I just wanted to make the
point. Good luck. TB


} Jondo,?------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123




From daemon Thu Dec 28 11:09:31 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Thu, 28 Dec 2000 11:52:29 -0500
Subject: Uranyl Acetate in Acetone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Out There:

I am currently involved with a cryo substitution project with human red
blood cells. I have replaced the non crystalline ice with 2% Osmium in 100%
acetone and have OK morphology, but the contrast is a bit low. I am
thinking about trying to follow the Osmium step with uranyl acetate, before
embedding in plastic. Does any one out there know if 1%uranly acetate will
be OK in 100% acetone? Thanks, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Fri Dec 29 08:52:00 2000



From: msoulek-at-foodprorecruiters.com
Date: Fri, 29 Dec 2000 08:28:33 -0600
Subject: Available position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: msoulek-at-foodprorecruiters.com
Name: Michael Soulek

Available position - Requesting interested parties or help with this
assignment.


I am looking for a person to fill an available Sr. Microscopist position in
the Midwest. Salary of $60-75k and a 5% bonus potential. Desire PHD or
equivalent experience in Histo and or Micro Chemistry area of knowledge.
Selected candidate will work in a million dollar + lab in a service group
on problems that are brought to the group to solve. Solid communication
and management of priorities is strongly sought in this person.

Food or plant background is sought after experience. Company does work on
a World Wide Basis

Some of the projects are to work in coloring,marking,compound ID,looking at
the different types to identify the various types of
proteins,carbohydrates,lipids or elements of the various tissues. The last
hired person came from USDA.

Contact
Michael Soulek
FOODPRO Recruiters Inc
210-494-9272
msoulek-at-foodprorecruiters.com

---------------------------------------------------------------------------




From daemon Fri Dec 29 12:34:02 2000



From: Alexander Mironov :      mironov-at-alpha400.cmns.mnegri.it
Date: Fri, 29 Dec 2000 19:29:21 +0100 (MET)
Subject: Re: Uranyl Acetate in Acetone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Timothy,
We also worked with acetone and I have found that if one uses 4% OsO4 in
acetone the contrast should be rather good.

Happy New Year!

Yours, Sasha

On Thu, 28 Dec 2000, Timothy Schneider wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Out There:
}
} I am currently involved with a cryo substitution project with human red
} blood cells. I have replaced the non crystalline ice with 2% Osmium in 100%
} acetone and have OK morphology, but the contrast is a bit low. I am
} thinking about trying to follow the Osmium step with uranyl acetate, before
} embedding in plastic. Does any one out there know if 1%uranly acetate will
} be OK in 100% acetone? Thanks, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu
}
}
}



From daemon Fri Dec 29 16:00:06 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 29 Dec 2000 13:52:56 -0800 (Pacific Standard Time)
Subject: Re: Thickness Measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jondo,

Can you deposit your coating onto a sample of polymerized
TEM embedding media, then cut the sample at 90 degrees on a
microtome, then measure the thickness using a TEM?

Good luck,

Doug

On Thu, 28 Dec 2000 15:38:21 +0900
=?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?=
{jdyun-at-kyungnam.ac.kr} wrote:

}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe -- Send Email
} to ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Sergey, Tobias and other listers:
}
} Thank you for your kind replies.
} The equipment that you mentioned seems too expensive for
} me to use, especially when I need just a couple of times
} thickness measurement for the calibration of my sputter
} coater. Is there other way to measure the thickness?
} Tobias suggested to use ellipsometry technique because the
} metallic or carbon coating transmits light when it is very
} thin. Have any you ever tried this before?
}
} Best wishes,
}
} Jondo Yun
} Kyungnam University, Korea
} jdyun-at-hanma.kyungnam.ac.kr
}
} ====================================================================
} Hello Jondo
}
} It looks like nobody answer your question.
}
} There are special machines called "thickness monitor" on
} the market. You have to go to your preferable EM supplier
} and ask them about that. It's very usual piece of
} equipment. It measures directly the thickness of the
} deposited material on the sensor. Sensor is mounted inside
} the vacuum chamber close to your sample. It is pretty
} sensitive (down to the 0.1 nm). The cost - couple of
} thousand backs. If you have further questions - I'll be
} happy to help you.
}
} Have a good holiday season.
}
} Sergey.
}
} Sergey Ryazantsev, Ph.D.
} Electron Microscopy
} Department of Biological Chemistry, School of Medicine
} University of California, Los Angeles
} Box 951737
} Los Angeles, CA 90095-1737
}
} (310) 825-1144 (office)
} Pager: (310) 845-0248
} FAX: (310) 206-5272 (departmental)
} mailto:sryazant-at-ucla.edu
}
} ===============================================================
} Jondo,
} Keep in mind that unless you deposit a really thick
} coating, carbon or metal coats do transmit light so
} ellipsometry could work. I have no personal experience with
} this, I just wanted to make the point. Good luck. TB
}
}
} } Jondo,?------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America } To Subscribe/Unsubscribe -- Send Email
} to ListServer-at-MSA.Microscopy.Com } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } } Dear Listers:
} } } Can anybody suggest how to measure thickness of the
} conductive } coating for EM specimen? If it is transparent,
} ellipsometer could be } used. Since metallic or carbon
} coating is not transparent, I could } not use that method.
} Then do I have only way to measure directly, } like
} measuring thickness on the cross-section or on the surface
} by } using EM or SPM? Is there any other way?
} } } Jondo Yun
} } jdyun-at-kyungnam.ac.kr
}
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ 109
} Tucker Hall / / / / \ \ \
} Biological Sciences /_ / __ /__ \ \ \__
} University of Missouri / / / \ \
} \ Columbia, MO USA / / /
} \ \ \ 65211-7400 / / ___ / \
} \__/ \ ____ voice: 573-882-0173 fax:
} 573-882-0123
}
}
}

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org








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