PCB's were a real concern even in the early 70's. Your oil tank may or may not have PCB's at all as I believe they were banned at that time. The procedure we used (in the early 70's) was to first confirm the PCB's by testing. Several outside labs tested for a modest fee.
I really don't know who would test nowadays. Maybe someone on the listserver would know.
Earl
----- Original Message ----- } From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, January 31, 2001 2:33 PM
At Oklahoma State they have a group that disposes of what ever you have as long as you know what it is and were it came from. You don't need to know if it has in it just the source. High voltage oil would be good enough for them. They do the testing if needed. It is a lot cheaper for the university for one office to deal with it than a bunch.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
----- Original Message ----- } From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, January 31, 2001 4:33 PM
TO: THOSE INVOLVED IN MICROSCOPY OF INVERTEBRATES
CONFERENCE ANNOUNCEMENT
The 9th International Congress on Invertebrate Reproduction & Development is to be held in South Africa (Rhodes University, Grahamstown) from July 15-20th. This integrative meeting welcomes papers on all aspects of invertebrate reproduction. The second circular with details is available at the conference web site: http://www.rhodes.ac.za/conferences/icird2001 where it is also possible to register online. The deadline date for registration and submission of abstracts is March 31st after which a late fee will apply. The current exchange rate is very much in favour of overseas delegates. With registration delegates get entrance to all conference sessions, daytime refreshments, lunches, conference literature and evening functions. Delegates can also book excursions to some of the local game parks where the "big five" can be seen in malaria-free reserves. The International Society for Invertebrate Reproduction was founded in 1975 - come to Africa to celebrate 25 years of the society. We want to know about your research on invertebrate reproduction.
If you require further information please do not hesitate to contact the conference organiser, Alan Hodgson (A.Hodgson-at-ru.ac.za)
Alan Hodgson -------------------------------------- Professor Alan Hodgson Dept. Zoology & Entomology Rhodes University Grahamstown 6140 South Africa Tel. (+46) 6038526 Fax (+46) 6224377 Cellphone 083 598 4892
Convener 9th International Conference on Invertebrate Reproduction & Development 15-20 July 2001. For information visit: http://www.rhodes.ac.za/conferences/icird2001/
} Date: Thu, 01 Feb 2001 10:25:14 +0100 } To: "Bob Price" {price-at-dcsmserver.med.sc.edu} , microscopy-at-sparc5.microscopy.com, confocal-at-listserve.acsu.buffalo.edu } From: Gareth Morgan {Gareth.Morgan-at-impi.ki.se} } Subject: EM Meeting in Stockholm } In-Reply-To: {041b81404221f11SERVICES-at-connect.med.sc.edu} } } Bob } } Thanks for the meeting announcement. Have you seen this one? } } http://www.biosci.ki.se/SCANDEM2001/ } } I hasten to add that I have no association with the conference. The conference scientific programme looks interesting and Sweden and Stockholm are worth a look. I know - I moved here 2 years ago. } } } }
"Words are the seeds of misunderstanding - use them carefully."
"Give us the wisdom to know and not to feel that not knowing is less than wisdom itself."
Gareth Morgan MPhil MSc FIBMS, Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5, Karolinska Institutet, Avdelningar för biomedicinsk laboratorievetenskap och biomedicinska ämnen, Lindhagensgatan 92, Box 12773, S 112 96, Stockholm Sverige
All due respect, but I'm not sure that anyone really has an answer to her question. I've had responses that said ESD effects are very similar to electron beam damage and I've had others who have said not. Frankly, I'm confused on the issue and would love some definitive response. Have one to offer?
On Monday, January 29, 2001 2:05 PM, Earl Weltmer [SMTP:earlw-at-pacbell.net] wrote: } } With all due respect (long pause): Isn't this thread getting a bit old? } } The person who asked this question has long since had her question answered. } } Doesn't this remind one of two dogs and only one tree? } } Thank You, } } Earl } } } } } Allen R. Sampson, Owner } } Advanced Research Systems } } 317 North 4th. Street } } St. Charles, Illinois 60174 } } voice 630.513.7093 fax 630.513.7092 } } } } } }
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174 voice 630.513.7093 fax 630.513.7092
Dear LISTERS-FRIENDS, Thank your very much to everyone for your kind replies on my problems with electron miocroscope JEM-4000EX.
There was problem in valve V4: the leak in the place of welding of internal bellow. This leak exist in open condition of valve only. We have changed this valve on new and now everything is OK. Again thank very much for help. Looking forward to help YOU in future.
Sincerely yours Anton Gutakovskii Laboratory of Electron Microscopy Institute of Semiconductor Physics Novosibirsk, Russia - mailto:gut-at-thermo.isp.nsc.ru
Can I refer you to an excellent article in Microscopy Today Issue 01-1. Page 28 (Rotary Shadowing Macromolecules) By Douglas R Keene.
drk-at-shcc.org
Hope it helps
Kind regards
Gareth Robinson
Gareth Robinson
Emitech Ltd - Serving the Science of Electron Microscopy South Stour Avenue Ashford Kent - TN23 7RS United Kingdom Tel +44 (0) 1233 646332 Fax +44 (0) 1233 640744 Email:EM-at-emitech.co.uk HTTP://www.emitech.co.uk
This communication contains information which is confidential and may also be privileged. It is for the exclusive use of the addressee. If you are not the addressee, please note that any distribution, dissemination, copying or use of this communication or the information in it is prohibited, and may be unlawful. If you have received this message in error please advise the sender
} -----Original Message----- } From: Heinrich Matthies [SMTP:hjmatthies-at-ucdavis.edu] } Sent: 31 January 2001 23:00 } To: Microscopy-at-sparc5.microscopy.com } Subject: rotary shadowing, platinum coating } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } } } Hello, } } I'm plan to attempt to rotary shadow a motor protein using platinum on } a } carbon rod and then coat with carbon. At the moment, I am trying to } evaporate the platinum from the carbon electrode and am having } difficulties. } } We have a denton vacuum LLC with a bench top turbo III high vacuum } evaporator. We wind one inch of platinum (Pt) wire on a nail (0.045) } and } then we load this 1 inch of 0.008 Pt wire onto a 0.04 tip of a 1.5mm } carbon } rod. The wire is pushed toward the solid carbon rod coming from the } other } side and all of the loops are tight (touch each other). The "spring" } of Pt } wire is tightly wound around the carbon wire. We pull a vacuum to } about 5 } x 10-5 torr and bring the amperage to 10 amps (filament 2) wait and } then } increase the amperage. Then I've tried 20,22.24, 26 and 30 amps but } usually somewhere between 26-30, the carbon appears to evaporate. At } lower } amps, less than 24, I don't see any Pt on the test paper. So at the } higher } amps, both carbon and pt evaporate and this means we are getting too } much } carbon rather than an initial Pt coating. Under these conditions, the } Pt } wire only covers a short distance of the narrow tip of the carbon } electrode. Does anyone have any advice or experienced this problem? } } Thank you, } } Heiner Matthies } UC Davis } MCB } 530-754-9051 } }
Earl ----- Original Message ----- } From: "Allen R. Sampson" {ars-at-sem.com} To: "'Earl Weltmer'" {earlw-at-pacbell.net} ; "'Mike Bode'" {mb-at-Soft-Imaging.com} ; "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, February 01, 2001 2:59 AM
Please post the following announcement:
POSITION AVAILABLE FOR EM TECHNICIAN The project involves localization of mRNA, cytoskeletal proteins and synaptic markers in neurons using in situ hybridization and immunogold methods. Expertise in immunogold and TEM desired. State of the art microscopy facility. Highly competitive salary and benefits.
Please send CV to:
Dr. Gary Bassell Department of Neuroscience Kennedy Center for Mental Retardation, #529 Albert Einstein College of Medicine 1410 Pelham Parkway Bronx, NY 10461 Tel 718-430-3648 FAX 718-430-2960 -- ------------------------------------ Gary Bassell, Ph.D Assistant Professor Department of Neuroscience Rose Kennedy Center for Mental Retardation, Room #529 Albert Einstein College of Medicine 1410 Pelham Parkway Bronx, NY 10461 Tel 718-430-3648 FAX 718-430-2960 http://www.aecom.yu.edu/asb/bassell/bassell.htm
I am seeking surplus TEM specimens for demonstration of EM to undergrad Biology students. We have acquired a Philips EM 300 which is operational but we would like to be able to show a range of biological preparations to our students. Currently we have no biological specimens whatsoever.
I would be grateful for any prepared grids which might be spared.
A brief description of a grid including a specimen's identity and perhaps the method of preparation would be useful.
Please reply off list if you might be able to assist. Thanks.
Darcy
Mr. Darcy Kehler, ext 3249 Lab Co-ordinator, Biology Trinity Western University 7600 Glover Rd Langley, BC Canada V2Y 1Y1 Switchboard: (604) 888-7511 Secretary: (604) 513-2043 fax (604) 513-2018
} } My EH&S guy wants to know about the current knowledge regarding the } disposal of HV tank oil that may be contaminated with PCB's. He has } an HV tank from a Philips x-ray machine, circa 1970's, rated at } 100KV. He asked me what is usually done to dispose of these tanks. } The information he finds is ambiguous. In some cases if the oil is } drained, it can be disposed of one way that may be cheaper than } getting rid of the whole thing intact. On the other hand, if he can } just get rid of the whole thing as one piece it would be a lot less } mess. } } Its the PCB's in the oil that complicates things. They were put in } most HV oils of that vintage, primarily to reduce the flamability of } the simple mineral oil used in the tank (I think). He would like to } avoid an assay for PCB's just to tell him something we already know. } Also, if there is really solid evidence and reasons for special care } and caution with th oil, it would help him chart the best course for } responsible disposal. }
You should first of all check with Philips, they are pretty together with info on that.
Electricity supply companies use a proprietary test kit to determine if there are PCBs in transformer oil, so contact your local.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Hello, I have a Durst Laborator 1200 varipoint enlarger. The Varicontrol 1200 frequently blows fuses. I am using a 1 amp slow blow fuse. The point source bulb is 100 watt. Can I get a replacement Varicontrol? Thank you, Sally Shrom
I would suggest looking at the power rating on the control unit. A 120-volt 100 watt bulb should pull 0.83 amps. That does not leave much margin or room for anything else. I would look at a 1.5A fuse if the unit rating permits it.
A on 120 V At 03:33 PM 2/1/2001 -0500, you wrote:
} Hello, } I have a Durst Laborator 1200 varipoint enlarger. The Varicontrol 1200 } frequently blows fuses. I am using a 1 amp slow blow fuse. The point } source bulb is 100 watt. Can I get a replacement Varicontrol? } Thank you, } Sally Shrom
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
The person who asked the inital question, Ginny Harper, is a customer of mine. She called me the day after her question & I directed her to proper, knowledgable, people at Motorola. As far as I know, her question has been answered to her satisfaction.
The last two emails were not directed at you personally. Like many on this Listserver I don't read every subject & delete the irrelevent ones as I am very busy. I took note at this one because a customer of mine asked the initial question.
I was surprised at the tangent(s) that this thread has generated none of which relate to her question. I believe this forum was meant to share collective knowledge & experiences. It appears to have degenerated to a test of "Will & Egos".
I decline your invitation to comment on said "ESD effects". I am an SEM repairman & "ESD effects" is not my area of expertise: nor is the subject of "quantum mechanics". Even the experts in the field of quantum mechanics dis-agree with each other. (I know this first hand). I will comment only on the areas of expertise that I feel I am qualified to comment. I will, however, direct you to people who are more qualified to answer that question as it is their business.
Regards,
Earl Weltmer
The last two comments were ----- Original Message ----- } From: "Allen R. Sampson" {ars-at-sem.com} To: "'Earl Weltmer'" {earlw-at-pacbell.net} ; "'Mike Bode'" {mb-at-Soft-Imaging.com} ; "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, February 01, 2001 2:59 AM
In order to meet federal requirements, the oil should be tested for PCB content. A result of less than 50 mg/kg is considered non-PCB. A concentration that 50 mg/kg to 499 mg/kg is considered to be contaminated with PCBs. And a concentration of 500 mg/kg or greater is considered to be PCB. There is an entire code in the Federal Register that deals with the proper way to dispose of these types of oil. If the concentration is found to be 50 mg/kg or greater, the oil is considered to be Hazardous Waste, and falls within the RCRA standard. The following URL will take you to the EPA's PCB Home Page where your friend will find several links to answer his questions.
http://www.epa.gov/opptintr/pcb/
Hope this helps.
Regards, Beth Bray
Elizabeth P. Bray Plant Chemist, Central Laboratory South Carolina Electric and Gas Co. 2102 N. Lake Dr. Columbia, SC 29212
----- Original Message ----- } From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, January 31, 2001 5:33 PM
I translate (x25.4) the funny measure 0.008 " to 0.2mm. For Pt/C coating the more commonly used thickness is 0.1mm wire. If you work out the cross section area of both wires you would find that you are using considerably more Pt than the 50 to 75mm of 0.1mm wire otherwise used. I suggest that you switch to the thinner wire because this would cover more of the 1.4mm carbon cylinder. Therefore this would take longer (in time) to evaporate. This is important because you need more than 5 seconds of evaporation to obtain an even coating - with the specimen rotating at perhaps 30 RPM. What do you mean: " } at lower amps, less than 24, I don't see any Pt on the test paper"? The C would be much more visible than the Pt and normally you would not see the Pt on filter paper. The test is in the looking. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Thursday, February 01, 2001 9:00 AM, Heinrich Matthies [SMTP:hjmatthies-at-ucdavis.edu] wrote: } } } Hello, } } I'm plan to attempt to rotary shadow a motor protein using platinum on a } carbon rod and then coat with carbon. At the moment, I am trying to } evaporate the platinum from the carbon electrode and am having difficulties. } } We have a denton vacuum LLC with a bench top turbo III high vacuum } evaporator. We wind one inch of platinum (Pt) wire on a nail (0.045) and } then we load this 1 inch of 0.008 Pt wire onto a 0.04 tip of a 1.5mm carbon } rod. The wire is pushed toward the solid carbon rod coming from the other } side and all of the loops are tight (touch each other). The "spring" of Pt } wire is tightly wound around the carbon wire. We pull a vacuum to about 5 } x 10-5 torr and bring the amperage to 10 amps (filament 2) wait and then } increase the amperage. Then I've tried 20,22.24, 26 and 30 amps but } usually somewhere between 26-30, the carbon appears to evaporate. At lower } amps, less than 24, I don't see any Pt on the test paper. So at the higher } amps, both carbon and pt evaporate and this means we are getting too much } carbon rather than an initial Pt coating. Under these conditions, the Pt } wire only covers a short distance of the narrow tip of the carbon } electrode. Does anyone have any advice or experienced this problem? } } Thank you, } } Heiner Matthies } UC Davis } MCB } 530-754-9051 } }
Hi, I am a student doing a Research paper on the subject ofLithography and Semiconductor Fabrication I need information on the subject of=20 Lithography....Its types specially Photolithography, Electron beam Lithograaphy, UV lithography and Xray lithography Plz help me...if u have any articleds plz send them to me... or tell me of WEB Resources that can help.. plz reply soon u980013-at-giki.edu.pk
I have used CD 34 QBend10 clone, to demonstrate endothelial cells for several years. It gave a clean picture with no problems.
3 months ago it started to pick up collagen, elastic and general connective tissue at such a high intensity that the technique became almost unusable.
I have changed the primary antibody and every other reagent involved in the technique. I have also tried changing the dilution factor and various blocks and pretreatments. Nothing has changed in our processing or fixation routines, and the problem is also occurring in blocks that had previously stained without the heavy background.
I am just about out of ideas, any thoughts would be appreciated.
Is there a place where you can purchase ready-made examples of things to look at with an SEM?
I'm just getting started with learning to use an SEM and I've been thinking that this would be helpful to have something to look at, that you already know what it should look like.
I am not familiar with technique you described, but let me comment your message a little bit. First of all, it's not clear to me: are you going to shadow your sample with Pt and then coat it with carbon or you want to perform Pt/C shadowing? I assume, you want to try the second one. Pt/C shadowing gives a less developed granularity because carbon protects Pt from aggregation/clusterization. The best solution for Pt/C shadowing - to use electron gun with special Pt/C insert. Many years ago, I did Pt/C thermal evaporation. For that purpose I did make special carbon rods 1.5 mm dia with hole. I was using hole to insert Pt wire inside the carbon rod. As long as you are using the same rod you will have highly reproducible results. Your set-Up seems to me is so tricky and less reproducible. About thickness: it's impossible to distinguish Pt from C in the mixed evaporation. Reasonable thickness will be when you just start to see the difference between exposed and unexposed to the shadowing area of paper. It will be something brownish, never black (it's too-o-o-o much!). If you are planning not only shadow your sample but see some details on it, vacuum in the range of 10-5 torr is not adequate. You, probably have to go to 2x10-6 torr at least. In general, the cleanness of the system is very important for shadowing. And the last: I would recommend you will practice a little bit with latex beads before start real experiment. The latex suspension is widely available from any EM supply vendors. You may chose the latex size correlated to your real molecules size. With latex, you will see perfectly the quality of your shadowing. Doing one-directional shadowing - you may determine the real angle of shadowing and the quality of the shadowed metal layer (for the real sample you may switch easily to the "rotary"). And the very last comment: rotary shadowing comes from DNA analysis. At that case it was necessary, because of elongated DNA shape and necessarily to trace the whole thing. For compact globular objects, there is no advantage for rotary shadowing. One or dual (perpendicular) shadowing may provide to you more information than rotary. Usually, the contrast of the rotary shadowed samples is less than for one/dual direction shadowing (at the same metal thickness). The geometry of the shadow may give you unique information about 3rd dimension of your object. I suggest you may try both.
Good luck!
Sergey
At 04:59 PM 1/31/01 -0600, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
John, We recently disposed of a Philips EM-200 TEM (circa 1963) and 3 others in storage for parts. That left us with 8 high voltage tanks...4 in power cabinets and 4 in scope consoles. Our radiological and environmental management people, responsible for disposing of all hazardous waste on campus, checked the tanks for PCB's and then disposed of the oil and tanks. This is part of their job and there was no charge to us. Do check with your people on campus....I suspect that they also have the means to take care of the problem for you. Debby
Debby Sherman Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-099e Whistler Building West Lafayette, IN 47907 --------------------------------------
In order to meet federal requirements, the oil should be tested for PCB content. A result of less than 50 mg/kg is considered non-PCB. A concentration that 50 mg/kg to 499 mg/kg is considered to be contaminated with PCBs. And a concentration of 500 mg/kg or greater is considered to be PCB. There is an entire code in the Federal Register that deals with the proper way to dispose of these types of oil. If the concentration is found to be 50 mg/kg or greater, the oil is considered to be Hazardous Waste, and falls within the RCRA standard. The following URL will take you to the EPA's PCB Home Page where your friend will find several links to answer his questions.
http://www.epa.gov/opptintr/pcb/
Hope this helps.
Regards, Beth Bray
Elizabeth P. Bray Plant Chemist, Central Laboratory South Carolina Electric and Gas Co. 2102 N. Lake Dr. Columbia, SC 29212
----- Original Message ----- } From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, January 31, 2001 5:33 PM
Walter McCrone has published a Particle Atlas that has lots of SEM images and their EDX spectra and there are also light images of the same materials, its been very useful reference for me. Its on a CD called PAE2 Particle Atlas. You can then collect the same materials and see what they look like on your systems or McCrone also sell collection of materials you could buy. I have collected samples of materials associated with the paper, printing/plating industry and EHS (office dusts) since that is what I look at most of the time . I have no interest in McCrone. I just use their stuff. Terry Ellis Hallmark Cards Inc. tellis2-at-hallmark.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Reply to: RE: LM. CD 34 help
Gramp Skin Pathology wrote: } I have used CD 34 QBend10 clone, to demonstrate endothelial cells for } several years. It gave a clean picture with no problems. } } 3 months ago it started to pick up collagen, elastic and general connective } tissue at such a high intensity that the technique became almost unusable. } } I have changed the primary antibody and every other reagent involved in the } technique. I have also tried changing the dilution factor and various blocks } and pretreatments. Nothing has changed in our processing or fixation } routines, and the problem is also occurring in blocks that had previously } stained without the heavy background. } } I am just about out of ideas, any thoughts would be appreciated.
This problem will have a logical explanation for why your labeling method has stopped working. It will be based either on something you changed (so look VERY carefully at the protocol actually being used) or something that has happened to either the reagents or the samples. As the problem occurs with samples that previously worked well, then it seems that specimen preparation can be ruled out as a source. Therefore you must look carefully at the reagents and the treatments you perform on the sections.
If the fixation protocols, the tissues being used and the labeling protocols are all the same (ie there have been NO changes), then the question to ask is: how do you store and handle your antibodies?
If there is no doubt that the antibody storage is not the problem, then the next question is: what is the blocking agent being used and has that been changed recently?
Regards,
Paul Webster
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
Gramp Skin Pathology wrote:
}
} } } } Mark Daymon } } } } } } RFC822 header } ----------------------------------- } } Received: from ultra5.microscopy.com [206.69.208.10] by mailhouse.hei.org } with ESMTP } (SMTPD32-6.00) id A9D026B501B2; Fri, 02 Feb 2001 01:11:44 -0800 } Received: (from daemon-at-localhost) } by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id UAA26664 } for dist-Microscopy; Thu, 1 Feb 2001 20:11:54 -0600 (CST) } Received: from no_more_spam.com (sparc5 [206.69.208.10]) } by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id UAA26661 } for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Thu, 1 Feb 2001 } 20:11:24 -0600 (CST) } Received: from mta04.mail.mel.aone.net.au (mta04.mail.au.uu.net [203.2.192.84]) } by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id UAA26654 } for {Microscopy-at-MSA.Microscopy.com} ; Thu, 1 Feb 2001 20:11:11 -0600 (CST) } Received: from computer-8 ([203.55.242.15]) by mta03.mail.mel.aone.net.au } with SMTP } id {20010202010416.XZGN19418.mta03.mail.mel.aone.net.au-at-computer-8} } for {Microscopy-at-MSA.Microscopy.com} ; } Fri, 2 Feb 2001 12:04:16 +1100 } Message-ID: {000201c08cb4$6b7c8b20$0ff237cb-at-computer-8} } From: "Gramp Skin Pathology" {grampath-at-camtech.net.au} } To: "mico bulletin board" {Microscopy-at-sparc5.microscopy.com} } Subject: LM. CD 34 help } Date: Fri, 2 Feb 2001 09:36:54 +1030 } MIME-Version: 1.0 } Content-Type: text/plain; } charset="iso-8859-1" } Content-Transfer-Encoding: 7bit } X-Priority: 3 } X-MSMail-Priority: Normal } X-Mailer: Microsoft Outlook Express 4.72.3110.5 } X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3110.3 } Errors-to: Microscopy-request-at-sparc5.microscopy.com } X-RCPT-TO: {pwebster-at-hei.org} } X-UIDL: 273279495 } Status: R }
by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id MAA28173 for dist-Microscopy; Fri, 2 Feb 2001 12:51:41 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id MAA28165 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Fri, 2 Feb 2001 12:51:11 -0600 (CST) Received: from mclean.mail.mindspring.net (mclean.mail.mindspring.net [207.69.200.57]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id MAA28156 for {Microscopy-at-sparc5.microscopy.com} ; Fri, 2 Feb 2001 12:50:59 -0600 (CST) Received: from Sony (user-2injhgr.dialup.mindspring.com [165.121.198.27]) by mclean.mail.mindspring.net (8.9.3/8.8.5) with SMTP id NAA26416 for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 2 Feb 2001 13:49:21 -0500 (EST) Message-ID: {000c01c08d48$ed79ad30$8a68fea9-at-Sony}
Thanks to all...
I think I'll just use the light bulb filament to see if everything works.
But, I'm interested in using this SEM to view fungi.
I'd like to start a small catalogue of these things.
This is probably on the intenet already?
----- Original Message ----- } From: "White, Woody N." {nwwhite-at-mcdermott.com} To: "'rad0'" {rden25-at-mindspring.com} Sent: Friday, February 02, 2001 7:39 AM
Royal Microscopical Society
12th International Conference on
MICROSCOPY OF SEMICONDUCTING MATERIALS
25-29 March 2001, University of Oxford, UK
******************************** Final Announcement ********************************
This international conference will focus on the latest developments in the study of the structural and electrical properties of semiconductors by the application of transmission and scanning electron microscopy, scanning probe microscopy and X-ray techniques.
The state-of-the-art in all important subject areas will be addressed, including the characterisation of bulk and thin film as-grown materials, the study of lattice defect and impurity behaviour and the investigation of advanced semiconductor processing procedures.
Special conference sessions will concentrate on recent developments in high-resolution imaging and analytical electron microscopy, advances in SEM and SPM applications, the characteristics of epitaxial layers (including III-V nitrides), quantum wells, wires and dots, the effects of device processing treatments (including, especially, advanced silicon technology) and metal-semiconductor contacts and silicides. Prominent invited speakers will introduce each topic area.
The full conference programme, together with registration information, is now available on the web site:
http://www.rms.org.uk/currentevents2.htm#MSMXII
Please contact the Royal Microscopical Society or the under- signed for any additional information.
Tony Cullis MSMXII Co-Chairman
**************** Professor Anthony G Cullis Head of Semiconductor Materials & Devices Group Dept of Electronic & Electrical Engineering University of Sheffield Mappin Street Sheffield S1 3JD, UK
I would like to encourage people to attend the forthcoming Interamerican Microscopy Congress. Previous meetings have been great fun and scientifically rewarding. Details follow. Alwyn Eades
VI Interamerican Congress on Electron Microscopy
October 7 - 11 2001 Hotel Emporio Veracruz, México
The Interamerican Congress on Electron Microscopy was previously held in Merida Venezuela, 1992; Cancún, México, 1993; Cancún, México, 1995; Guayaquil Ecuador, 1997 and Margarita Island Venezuela, 1999 This is the official congress of Committee of Inter-American Societies for Electron Microscopy (CIASEM) and is a forum for microscopy in The Americas. The Congress will have plenary talks, invited talks and a poster session. Proceedings will be published.
Congress Site The congress will take place at Hotel Emporio, which has a fine location in the port of Veracruz: It is in the heart of the historic center. The tourist attractions of the port of Veracruz and surroundings range from a rich nightlife to sites of unmatched natural beauty. It was the home of the Olmeca culture and is where Hernán Cortés disembarked. There are wonderful beaches. The well-preserved archeological areas, vast tropical forests, and colonial cities make Veracruz a fascinating place to visit.
Conference Topics:
Materials Science Electron Microcopy of magnetic materials, fracture mechanics, thin films and semiconductors, materials, computer simulation, sol-gel materials, polymers, ceramics, glasses, renewable energy materials, recycling materials, surfaces characterization, corrosion, composites, and general topics in material science.
Biological Sciences In situ hybridization, immunolocalization, scanning probe microscopy in biology, pathology, microscopy and cell biology, neurobiology, and general topics in biological sciences
Short Courses and Labs: Sample preparation Scanning microscopy TEM and High Resolution Digital and Image Processing Advances in remote control electron microscopy
Exhibition Electron microscopes, sample preparation and related products will be displayed during the congress. Exhibitors in fields related to new microscopies and optical microscopies are encouraged to participate.
Call for papers Abstracts must be contained in exactly 2 pages. The first page will include only text, including title, authors, affiliation, main body of the work and references. The second page will include text, tables and figures. Use a word processor, with high quality printer, TIMES NEW ROMAN type letter in 12 points, and a single space line. Abstracts will be published in Acta Microscopica
Deadline to send abstracts is July 15, 2001.
English will be the official language of the Congress. Translation facilities will not be available.
Hotel Reservation Special rates are available at the Hotel Emporio for Congress attendees. For reservations contact the hotel directly mentioning the congress. Single rooms are US $98, Doubles $108.50, extra people $17. Suites are $116 (single) and $138 (double).
Before august 31, 2001 $ 300.00 USD Students: $ 100.00 USD After august 31, 2001 $ 325.00 USD Students: $ 125.00 USD Courses Before august 31, 2001 $ 60.00 USD After august 31, 2001 $ 80.00 USD
Chairman: Miguel José Yacamán (yacaman-at-che.utexas.edu)
Informations and Registration Dr. José Reyes-Gasga Instituto de Física, UNAM Apartado Postal 20-364, 01000 México D.F., México Phone: (525) 622-5083 Fax. (525) 622-5008 Email: jreyes-at-fenix.ifisicacu.unam.mx
Could anyone provide me with the telephone number of the Laboratory of Scanning Electron Microscopy at the Harvard Museum of Comparative Zoology? Many thanks in advance.
I have a model 903/904 AO automatic knife sharpener. I am missing the redressing bridge for reconditioning the glass plates. Does anyone have one they would like to get rid of?
Kreonite is pretty much a notable industry standard for paper and emulsion autoprocessing. These are not low cost units, however. Rebuilt ones can be found at places like:
http://www.dunningphoto.com/rebuilt.html
You can also search for other sources using the key model numbers which suit your needs.
gary g.
At 01:33 PM 1/30/01, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Try Media Cybernetics Image Pro-Plus. Then add an NTSC frame grabber card which it supports. The Matrox and National Instruments cards are very good. with this complement, you can average, integrate, Kalman and do pixel mapping as well as image archiving from a standard TV video source.
http://www.mediacy.com
gary g.
At 07:03 AM 1/29/01, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Email: carlos-e-chavez-at-uiowa.edu Name: carlos e chavez,dds School: University of Iowa College of Dentistry State: Iowa
I am an graduate student currently enrolled in a Master's program in Operative Dentistry at University of Iowa, College of Dentistry. My research interest involves the evaluation of interfaces between composite resins used in dentistry and a noble (Au 51.5%, Pd 38%) and base metal alloys (NiCrBe). I have mounted a metal disc of 10mm. diameterX 2mm thick (Noble or base alloy) in Epoxy resin and followed the protocol used in dental research for metal preparation (280, 400,and 600 grit Si Carbide paper under running water, etc). Then, using a brush I painted on the metal surface delimited by a tape with a 6 mm diameter hole using a resin opaquer paste of 0.5 mm thick. Then using a mold of 2.38 mm diameter a applied a composite resin and light cured to harden it.Finally, I cleaned the excess opaque in the periphery. My question is how do you prepare this three element interface for observation under the SEM or optical microscope? How would I make the cuts so I do not disturb the bond between the three elements and examine at these interfaces under the microscope accurately?. I hope this is clear. Thank you very much. Carlos E. Chavez, DDS University of Iowa College of Dentistry
The "main" way Pt/C I thought was being evaporated today is the "bead on carbon" method, that is, where the wire is coiled up, around a pin of diameter slightly larger than the diameter of the neck on the carbon rod, and then, with some help from some good Style #5 tweezers, it is pulled onto the sharpened neck of the carbon rod. The bell jar is lowered over the carbon rods and holders, and wearing the appropriate eye protection, the power is slowly turned up, slowly heating the carbon rods and wound Pt wire. At some point the miracle happens: The Pt melts, and surface tension effects cause it to form (in an instant) a nice little "bead" (it looks like the textbook "sessile drop") firmly attached to the carbon rod once the heating is stopped. After cooling down, the rod with the drop is rotated around so that it is facing where the samples will be, the bell jar is then loaded, pumped down and evaporation of Pt/C can be done simultaneously this way.
Indeed I don't think it is possible to evaporate Pt wire **without** the formation of the sessile drop, therefore, how the wire is originally spread out (on the rod neck) does not matter!
A note not related to the original question: The sharpened "neck" should be sharpened to a diameter not more than 3/16" (4.75 mm). The method will not work as well if the neck diameter is larger than this. If you are sharpening your own rods, make sure you are using rods of higher rather than lower density, otherwise the rod will not have the mechanical properties needed to sharpen down to this diameter.
I had a phone call on Friday from a customer asking about 0.1 mm (e.g. 4 mil ) wire since a previous posting seemed to attribute to it something special (relative to 0.2 mm/8 mil) wire. I told the caller that we did not believe that to be so, that for the practice of the sessile drop method, either diameter wire would "bead up" just as quickly and easily, and the only difference between the two diameters would be one of cost, since the cost to draw the same mass in 4 ml is obviously going to be higher than that to draw 8 mil. Actually some years ago we came into some 10 mil Pt wire, and we found it could be used just as easily to make the sessile drop.
Furthermore since either diameter wire could be used to create a drop of equal size, the actual evaporation time would be independent of the diameter of the starting wire.
In the end, we came upon the novel conclusion that I would make a posting and subject my advice to the most stringent (microscopy) peer review panel in the world, namely the listserver. Am I not correct, in that the end result will be the same irrespective of whether 4 mil or 8 mil wire is used?
Chuck
Disclaimer: SPI Supplies is a long time supplier of the high purity Pt wire that is used for evaporation in electron microscopy applications.
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
Handbook of Microlithography, Micromachining, and Microfabrication : Microlithography by P. Rai-Choudhury (Editor) Hardcover Vol 001 (June 1997) SPIE Press; ISBN: 0819423785
This is a very thorough book which covers optical lithography, electron beam lithography, and x-ray lithography, as well as other related topics. The chapter on e-beam lithography is on the web at "http://www.cnf.cornell.edu/spiebook/toc.htm".
A list of other books that may be useful can be found at "www.jcnabity.com/booklist.htm".
Joe _________________________________________ Joe Nabity, Ph.D. JC Nabity Lithography Systems E-Beam Lithography using Commercial SEMs & STEMs PO Box 5354, Bozeman, MT 59717 USA Voice: (406) 587-0848 FAX: (406) 586-9514 E-mail: info-at-jcnabity.com Web: www.jcnabity.com
In a message dated 2/1/2001 11:25:38 PM Mountain Standard Time, u980013-at-giki.edu.pk writes:
} Hi, } I am a student doing a Research paper on the subject ofLithography and } Semiconductor Fabrication } I need information on the subject of=20 } Lithography....Its types specially Photolithography, } Electron beam Lithograaphy, UV lithography and Xray lithography } Plz help me...if u have any articleds plz send them to me... } or tell me of WEB Resources that can help.. } plz reply soon } u980013-at-giki.edu.pk }
Email: Rassad-at-students.miami.edu Name: Rizwan Assad School: University of Miami
Question: I would like to know if I would be able to see a protein that weighs about 100 kilo daltons under an SEM. If so, where can I find resourses for the preparation of the specimen?
I ran across an original manual for an Oxford Vibratome--Model G-Catalog no. 501 and 502. Since we no longer have the vibratome, we don't need the manual. Does anyone want it?
Bob Robert R. Wise, Ph.D. Associate Professor of Plant Physiology Department of Biology and Microbiology University of Wisconsin Oshkosh Oshkosh, WI 54901 tele: (920) 424-3404 fax: (920) 424-1101 wise-at-uwosh.edu http://www.uwosh.edu/departments/biology/wise/wise.html
You can also look at www.integraltech.com for very reliable image capture cards. They have 1000's installed (we have sold that many alone). They have AGP and PCI versions. Also can handle signals from RGB to NTSC Composite.
As for software -- there is a product called A4i that is pretty good (not ours) and www.paxit.com (ours) that handle all of the archiving, measuring, Excel interface, report generation and image analysis functions you are most likely desiring. We have thousands of systems in stalled and many in semicon mnfg and QA. Both of these products have network versions that allow less expensive licenses for your work outside the fab.
Try Media Cybernetics Image Pro-Plus. Then add an NTSC frame grabber card which it supports. The Matrox and National Instruments cards are very good. with this complement, you can average, integrate, Kalman and do pixel mapping as well as image archiving from a standard TV video source.
http://www.mediacy.com
gary g.
At 07:03 AM 1/29/01, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
there are definitely many cards out there to acquire standard TV signals from any source that adheres to that standard. These standards were developed decades ago for TV cameras, and my personal opinion is, that they are good for moving images (as on TV), but they lack resolution and definition for still images (LM and SEM). However, since they cards are usually not very expensive, and some of them do offer integration capabilities (normally frame integration), it might be worth a shot. Also the microscopes themselves might be offering integration capabilities. You are limited to a resolution of 640x480 (NTSC) or 758x576 (PAL).
For better resolution you might want to look at other options as well. For SEMs there are interfaces available (such as our ADDA II, but there are other manufacturers also), normally available as "passive" or "active" systems. For LM, of course, there are digital cameras with better resolution and bit-depth (Video only carries about 8 bits of information, if you're lucky).
Look for systems that are open for upward expansion to keep your options open. Contact me offline if you are interested in getting more information about the way we do that.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
At 07:03 AM 1/29/01, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I would like to request of preparation samples from poly(styrene)/poly(butyl acrylate) dispersion with 100 nm particles size for measurements by JEOL type of transmission electron microscope. We have got some problems with dilution of latexes and with time of staining of carbon, too. We would like to confirm a core/shell particles morphology of samples prepared by two step seeded emulsion polymerization. We have got some problems with magnification and contrast expansion of core/shell particles,too. Thank you very much for your help.
Petra Volfova, PhD. student of Department of plastics and rubber, Slovak Technical University,Bratislava,Slovak Republic.
MIDWEST MICROSCOPY AND MICROANALYSIS SOCIETY, INC. (MMMS) AFFILIATE OF THE MICROSCOPY SOCIETY OF AMERICA
MEETING ANNOUNCEMENT
JOINT MEETING WITH THE SOCIETY FOR APPLIED SPECTROSCOPY Tuesday, Feb. 13, 2001
At Unilever HPC USA, located at 3100 Golf Road, Rolling Meadows, IL. See directions for more details.
Social Hour: 5:30 pm Dinner: 6:00 pm Speaker: 7:00 pm _________________________________________________________________ The Infrared Microprobe in Production and Research By Koichi Nishikida
Abstract Unlike the visible microscope and electron microscope, which respectively use visible light and electrons to magnify the sample, the infrared microscope does not utilize infrared wavelengths to magnify the sample image. Instead, a visible microscope is modified, so that a magnified sample image is observed and, at the same time, an infrared beam is passed through the microscope to obtain an infrared spectrum of the sample. Therefore, the "IR microscope" should be better named the "IR microprobe".
Applications of the IR-microprobe cover applications from forensic } analysis to defect analysis, reverse engineering in the production industry, and medical diagnostic research.
In this talk, I will show how the IR-microprobe has contributed to quality improvements in the production industry, as well as recent research in medical applications. Briefly, I will address the evolution of this technique over the past five decades. ********************************************************************* Please make your dinner reservations for the upcoming meeting by calling (847)734-3712. Leave your name, company affiliation and the number of reservations. Please call by noon on February 9th, so that proper arrangements can be made. Dinner Cost: M3S Members: $25 M3S Students: $10 Nonmembers: $30
Directions to Unilever, Rolling Meadows, Illinois
From the Chicago Area: Kennedy Expressway to I-90 West (Rockford); I-90 West to Arlington Heights Rd.; North to Golf Rd. (2nd traffic light); Turn left (West) onto Golf Rd. for approximately 2 miles. The Unilever R&D facility is on the right, just before Hwy. 53, across from the forest preserve. Follow the entrance road all the way to the last parking lot (North lot) and enter the building through the far glass doors.
From I-290 Eisenhower Expressway: Stay on I-290 towards Rockford; Exit on Golf/Higgins Rds.; Continue North to Golf Rd.; Turn east on Golf Rd. Unilever is1/4 mile left on Golf Rd., across from the forest preserve. Follow the entrance road all the way to the last parking lot (North lot) and enter the building through the far glass doors.
From the Northern Suburbs (Route 53 South): Exit Woodfield Drive/Golf Rd. (58); Continue to first light; Turn left at first light; Continue to Golf Rd (58); Turn right (East) on Golf Rd.; Unilever is1/4 mile left on Golf Rd., across from the forest preserve. Follow the entrance road all the way to the last parking lot (North lot) and enter the building through the far glass doors.
If you have any questions about the meeting, please direct your inquiries to the phone number below. For questions about MMMS, please contact:
Dr. Damian Neuberger Senior Research Scientist Baxter Healthcare, Corp. (847) 270-5888 damian_neuberger-at-baxter.com
} Email: kmccleary54-at-hotmail.com } Name: Kevin McCleary } School: South Peace Secondary School } State: British Columbia, Canada } } Question: I am anticipating the purchase of prepared slides for an } introduction to cells in my grade ten science classes.Ý Will chromosomes be } easily viewed in smears of Drosophila (advertized as giant chromosomes)? } If not, could you suggest alternate subjects.Ý Our light microscopes are of } standard high school quality. } _________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.
Chuck's reply without the customary inclusion of the previous contribution is difficult to understand. I have now added my previous contribution and the original request below. The hanging drop method results largely in Pt evaporation followed by carbon. The method also requires breaking the vacuum and is therefore more trouble. As Sergey Ryazantsev in his thoughtful contribution explained (I erased that because there was no need to reply), its the simultaneous nature of Pt/C which yields the finer grain structure. Sergey also explained that the other means of achieving such fine grain is electron beam evaporation, but that requires special equipment. I explained in my previous contribution why the thinner wire is required. 30 years ago I used many meters annually of the 0.1mm Pt for freeze etching and Kleinschmitt/ DNA rotary shadowing. Nothing for it Chuck: you will need to stock 0.1mm Pt wire like all other major suppliers. Don't be overly concerned, very few people use these techniques now; in fact you could purchase your minor requirements from us! Disclaimer: its too obvious, we sell small quantities of 0.1mm Pt. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Sunday, February 04, 2001 12:43 AM, Garber, Charles A. [SMTP:cgarber-at-2spi.com] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } The "main" way Pt/C I thought was being evaporated today is the "bead on } carbon" method, that is, where the wire is coiled up, around a pin of } diameter slightly larger than the diameter of the neck on the carbon rod, } and then, with some help from some good Style #5 tweezers, it is pulled onto } the sharpened neck of the carbon rod. The bell jar is lowered over the } carbon rods and holders, and wearing the appropriate eye protection, the } power is slowly turned up, slowly heating the carbon rods and wound Pt wire. } At some point the miracle happens: The Pt melts, and surface tension } effects cause it to form (in an instant) a nice little "bead" (it looks like } the textbook "sessile drop") firmly attached to the carbon rod once the } heating is stopped. After cooling down, the rod with the drop is rotated } around so that it is facing where the samples will be, the bell jar is then } loaded, pumped down and evaporation of Pt/C can be done simultaneously this } way. } } Indeed I don't think it is possible to evaporate Pt wire **without** the } formation of the sessile drop, therefore, how the wire is originally spread } out (on the rod neck) does not matter! } } A note not related to the original question: The sharpened "neck" should be } sharpened to a diameter not more than 3/16" (4.75 mm). The method will not } work as well if the neck diameter is larger than this. If you are } sharpening your own rods, make sure you are using rods of higher rather than } lower density, otherwise the rod will not have the mechanical properties } needed to sharpen down to this diameter. } } I had a phone call on Friday from a customer asking about 0.1 mm (e.g. 4 mil } ) wire since a previous posting seemed to attribute to it something special } (relative to 0.2 mm/8 mil) wire. I told the caller that we did not believe } that to be so, that for the practice of the sessile drop method, either } diameter wire would "bead up" just as quickly and easily, and the only } difference between the two diameters would be one of cost, since the cost to } draw the same mass in 4 ml is obviously going to be higher than that to draw } 8 mil. Actually some years ago we came into some 10 mil Pt wire, and we } found it could be used just as easily to make the sessile drop. } } Furthermore since either diameter wire could be used to create a drop of } equal size, the actual evaporation time would be independent of the diameter } of the starting wire. } } In the end, we came upon the novel conclusion that I would make a posting } and subject my advice to the most stringent (microscopy) peer review panel } in the world, namely the listserver. Am I not correct, in that the end } result will be the same irrespective of whether 4 mil or 8 mil wire is used? } } Chuck } } Disclaimer: SPI Supplies is a long time supplier of the high purity Pt wire } that is used for evaporation in electron microscopy applications. } } =================================================== } Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 } President 1-(800)-2424-SPI } SPI SUPPLIES FAX: 1-(610)-436-5755 } PO BOX 656 e-mail: cgarber-at-2spi.com } West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com } } } Look for us! } ############################ } WWW: http://www.2spi.com } ############################ } ==================================================
} From: Jim at ProSciTech [SMTP:jim-at-proscitech.com] Sent: Friday, February 02, 2001 9:59 AM To: 'Heinrich Matthies'; Microscopy-at-sparc5.microscopy.com
Folks,
Past sand donations from listserver subscribers helped start the MSA sand collection. This collection is freely given to educators to use with Microscopic Explorations and other educational programs. Recently, we advertised this resource to educators and the response has been overwhelming. Consequently much of the sand collection has been given away and the collection is in need of restoration. As you can guess, the most popular sands are from locations outside the United States.
So I am asking for donations from listserver subscribers, especially those outside the United States. At one point we had sand from every continent, but that is not the case any more. Please help us rebuild the MSA sand collection. Double bag your sand in sealable bags (Ziplock baggies work great) and mail your donations to:
Joe Neilly Abbott Laboratories D-45M, AP31 200 Abbott Park Rd. Abbott Park, IL 60064-6202 voice: (847)-938-5024 fax: (847)-938-5027 e-mail: joe.neilly-at-abbott.com
} From your description of the specimen preparation it seems you have prepared a sample for some type of mechanical test (shear bond test?) If you need just observe an interface you can prepare samples for this purpose. Do not embed you disks in Epoxy. Treat all disk surface with with resin and composite. On low speed diamond saw cut disks in halves. Then embed them in Epoxy and polish with diamond pastes. All adhesives and composites should withstand this treatment easily. The most difficult part is polishing - glass particles of a composite filling could produce a lot of scratches, so treat you sections gently.
Good luck,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 3127 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: "carlos-e-chavez-at-uiowa.edu"-at-sparc5.microscopy.com } [mailto:"carlos-e-chavez-at-uiowa.edu"-at-sparc5.microscopy.com] } Sent: Saturday, February 03, 2001 9:34 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Ask-A-Microscopist: Microscopy of Dental Interfaces } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } } Email: carlos-e-chavez-at-uiowa.edu } Name: carlos e chavez,dds } School: University of Iowa College of Dentistry } State: Iowa } } I am an graduate student currently enrolled in a Master's program in } Operative Dentistry at University of Iowa, College of Dentistry. My } research interest involves the evaluation of interfaces } between composite } resins used in dentistry and a noble (Au 51.5%, Pd 38%) and base metal } alloys (NiCrBe). I have mounted a metal disc of 10mm. } diameterX 2mm thick } (Noble or base alloy) in Epoxy resin and followed the protocol used in } dental research for metal preparation (280, 400,and 600 grit } Si Carbide } paper under running water, etc). Then, using a brush I } painted on the metal } surface delimited by a tape with a 6 mm diameter hole using a } resin opaquer } paste of 0.5 mm thick. Then using a mold of 2.38 mm diameter } a applied a } composite resin and light cured to harden it.Finally, I } cleaned the excess } opaque in the periphery. My question is how do you prepare this three } element interface for observation under the SEM or optical microscope? } How would I make the cuts so I do not disturb the bond } between the three } elements and examine at these interfaces under the microscope } accurately?. } I hope this is clear. } Thank you very much. } Carlos E. Chavez, DDS } University of Iowa College of Dentistry } } } } } } } } } -------------------------------------------------------------- } ------------- } } }
We are about to buy a Hamamatsu Orca CCD camera to capture images, viewed with both fluorescent and transmitted light. This seems to be a popular choice of camera among microscopists. We would like to know which are the different (favorite) software packages that people are using to collect, display and analyze the data captured by an Orca camera.
Thank you.
Judy Trogadis Vision Science Research Program Toronto Western Research Institute 399 Bathurst St. Toronto, ON M5T 2S8 ph: 416-603-5088 fax: 416-603-5126 email:judy-at-uhnres.utoronto.ca
Eric Cowdrey and I have put together a web page that makes it relatively easy to find the e-mail addresses of academic colleagues, for the purpose of requesting reprints:
There are no a d v e r t i s e m e n t s or costs. If you use it, please let us know of omissions, corrections, or improvements you'd like, and forward it to your colleagues. --
Dr. Richard Gordon, Radiology, University of Manitoba, HSC Rm. GA216, 820 Sherbrook St. Winnipeg R3A 1R9 Canada Adjunct: Electrical & Computer Engineering, Exec Member: CSTB, CARRF, IEEE-EMBS phone:(204)789-3828, fax:(204)787-2080, e-mail: GordonR-at-ms.umanitoba.ca New book: The Hierarchical Genome & Differentiation Waves: Novel Unification of Development, Genetics & Evolution: http://www.wspc.com.sg/books/lifesci/2755.html Finding Reprint Authors' E-Mail Addresses: http://www.umanitoba.ca/faculties/medicine/radiology/search/searchindex.html
SECOND ANNOUNCEMENT: UBC LIVE-CELL COURSE: Please register by March 1!!
Hello all,
The faculty for the 2000 UBC 3D Live-cell Microscopy Course is now almost set .
o Stephen Adams University of California-SD o Ping Chin Cheng SUNY, Buffalo o Elaine Humphrey Univ. of British Columbia o Stephan Hell Max Planck, Goettingen o Ted Inoué Universal Imaging, PA o Iain Johnson Molecular Probes, OR o Larry Keenan Cell Robotics, NM o Ernst Keller Carl Zeiss, Thornwood, NY o Andreas Kriete University of Geissen o Felix Margadant University of Sydney o Tim Murphy University of British Columbia o Jim Pawley University of Wisconsin-Madison o Steve Potter California Institute of Tech o Michael Weis Agriculture Canada
Basic info is below but you can get the entire brochure, including links to faculty home pages, at
Organized by Prof. James Pawley, (University of Wisconsin-Madison)
in association with Dr. Elaine Humphrey UBC BioSciences Microscopy Facility: University of British Columbia Vancouver, BC, Canada
DATES
Applications must be received by March 1, 2001 Deposit due April 15, 2001 Registration 5:00 - 7:00 PM Sunday, June 17, 2001 First Lecture 7:30 PM Sunday, June 17, 2001 Live-cell Course ends, noon Thursday, June 28, 2001 3D Image Processing Wksp Sat. June 30 - Monday, July 2
APPLICATIONS DUE BY MARCH 1, 2001
More information at : http://www.cs.ubc.ca/spider/ladic/course/bulletin.html
or
REGARDING COURSE ORGANIZATION:
Contact: Prof. James B. Pawley, JBPAWLEY-at-FACSTAFF.WISC.EDU
REGARDING APPLICATIONS
Dr. Elaine Humphrey, Biosciences EM Facility Biosciences Building Univ. of British Columbia 6270 University Blvd. Vancouver, BC, V6T-1Z4
THE PURPOSE OF THE COURSE
Modern methods of 3D light microscopy promise a revolutionary improvement in our ability to view living cells. To help convert this promise to reality for a wider selection of biological scientists, an intensive eleven-day residential course concentrating on all aspects of 3D Microscopy of Living Cells will be held at the University of British Columbia, in June of 2001. The course includes 4 days on 2D techniques, 6 days of 3D techniques and a summary presentation. It covers basic microscopy to the highest level confocal microscopy. (A half-day Pre-course is offered for any who may need to brush up on basic optics!).
Topics include: o Quantitative 2D light microscopy o How to keep your cells alive o 3D imaging in confocal microscopy o Widefield/deconvolution techniques o Two-photon excitation microscopy o Fluorescent and backscattered light signals o Dye design, characteristics and use o Pixelation: The Nyquist Criterion o Lasers and laser tweezers o Objectives and aberrations o Scanning-systems: AODs and mirrors o Optimal pinhole size/photon efficiency o Detectors: operation and performance o Video-rate confocal imaging o Measuring ion concentrations o Display and measurement of 3D data
Morning lecture/demonstrations lead to hands-on laboratory exercises each afternoon that will utilize most of the commercial instruments currently available for 3D microscopic imaging. Students will work in groups of 3 or 4 throughout the discussion and laboratory sessions, and may complete a live-cell 3D study on their own specimens. In the first five years, over 130 students from 23 countries have attended. Last year, 11 separate, 3D microscopical workstations were available for student use under the supervision of a 17-member international faculty. We expect to have even more workstations in 2001. Including manufacturers representatives, the teacher/ student ratio will be almost 2:1.
INTERNATIONAL FACULTY
o Stephen Adams University of California-SD o Ping Chin Cheng SUNY, Buffalo o Elaine Humphrey Univ. of British Columbia o Stephan Hell Max Planck, Goettingen o Elaine Humphrey University of British Columbia o Iain Johnson Molecular Probes, OR o Larry Keenan Cell Robotics, NM o Ernst Keller Carl Zeiss, Thornwood, NY o Andreas Kriete University of Geissen o Felix Margadant University of Sydney o Glen MacDonald University of Washington o Tim Murphy University of British Columbia o Jim Pawley University of Wisconsin-Madison o Steve Potter California Institute of Tech o Michael Weis Agriculture Canada
TUITION
Course tuition is $2,150 US and includes lunches. On receipt of 50% deposit, students will receive preliminary group assignments and the textbook, Handbook of Biological Confocal Microscopy, (Plenum, 1995). The tuition fee includes the textbook, a binder of handouts, and tickets for the Opening Reception, the Manufacturer's Reception and the Beach Party. Accommodations and meals other than lunch are not included in the tuition fee. The Pre-course is $100 US.
APPLICATIONS
Applicants will complete a questionnaire to assess knowledge level and field of interest. Enrollment is limited to about 24 participants. Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with basic texts on request to read before the course begins and are encouraged to take the Pre-course on the afternoon of June 18.
Application forms, and other course information from this and past years, can be downloaded from the WWW site at:
Dr. Elaine Humphrey, Biosciences EM Facility Biosciences Building Univ. of British Columbia 6270 University Blvd. Vancouver, BC, V6T-1Z4 Phone: 1-604.822-3354 FAX: 1-604.265-5315 Email: ech-at-unixg.ubc.ca.
Application deadlines:
Application forms are due by March 1, 2000! Deadlines are early to facilitate setting up groups. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 2000 to reserve your position. In general, deposit refunds are only be possible if your position can be filled from the waiting list. The remainder of the fees is due at registration.
DATES
Applications must be received by March 1, 2001 Deposit due April 15 2001 Registration 5:00 - 7:00 PM Sunday, June 17, 2001 First Lecture 7:30 PM Sunday, June 17, 2001 Live-cell Course ends, noon Thursday, June 28, 2001
TEACHING PHILOSOPHY
As befits teaching in an area at the boundary of "what is now known," lecturers have been chosen based on their expertise as scientists working in the field rather than because they all agree. They are encouraged more to be provocative than to be prosaic. Students should expect discussion in areas where differences of opinion exist.
Prior to the course, students will be organized into groups and encouraged to communicate by email/phone, about the "Living-cell" group projects that they will pursue during the course and that will be presented to the class on the last day of the course. It has been found that group interactions make best use of students' prior experience and can be very effective in teaching the practical skills covered in a hands-on course of this type.
ARRANGEMENTS FOR LIVE SAMPLES
Students must contact Dr. Elaine Humphrey to make necessary arrangements for the transport and maintenance of cell lines etc. needed for their projects. Organisms linked in any way with human disease are not permitted because of safety considerations. Transport and customs arrangements for living specimens are entirely the responsibility of the student. Students also attending the 3D Image Processing Course, may be able to analyze, process and display some of the 3D data collected from their specimens
The workshop will cover 3D image processing for measurement and display. Enrollment is limited to those attending the 3D Microscopy course. Tuition : $850 US (lunches and snacks incl.)
WHO SHOULD ATTEND?
The course is designed for biologists working with multidimensional and possibly multicolor microscopical data sets. Getting the data is only half of the battle. Image data in 3, 4 or even 5 dimensions may be difficult to store let alone analyze or display. This course is to help students understand the hardware and software aspects of this problem and give them the techniques they need to make the best use of their data.
The course is designed for biologists who need to make measurements on 3D microscopical data sets and then display the results in an effective manner. The course will be taught on SGI, Macintosh and IBM-compatible computers. A wide variety of software designed for the 3D microscopy market will be described, demonstrated and available for use.
Workshop Organizers
o Andreas Kriete Giessen University, DE o Felix Margadant University of Sydney, Au o Ping Chin Cheng State U. of New York, Buffalo o Elaine Humphrey University of British Columbia o Glen MacDonald University of Washington
PLAN OF INSTRUCTION Classes will meet from 8:30-12:00 and 1:00-6:00 with lecture-demonstrations followed immediately by hands-on laboratory sessions using a variety of workstations. Students will "learn-by-doing" with two to a machine. Lab handouts will describe some specific exercises to be performed on "canned" data sets. Facilities and supervision will be available until 11:00 PM, for students to work on their own data. -- **************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39
I found that the 990 has specific applications, rather than being of a broad nature. It is not good for DIC metallurgical work since color balance is not effective. It is not bad for BF. It does do a nice job on a stereo scope. with color balanced lighting, the auto white balance will work well. The auto exposure is also very functional. For focusing, I used the AF mode with the zoom set to wide and use the scope focus for all focusing. The LCD display works fine for focusing. The critical part is to use the remote release cable. Otherwise, operation is a real pain. I typically had to take 3-5 shots to get one that was acceptable. Just a minor inconvenience. I shot at high rez, JPEG. I have not tried high rez TIFF mode.
Manual operation may be an ultimately better approach, but I have not worked much with this mode. The 990 is OK for quickie shots and proofs but for my work, I need higher pixel resolution and the ability to control gamma, and highlight/shadow regions. Also, the 990 lacks the provision for specific manual color temperature setting on a per-region basis. This is not an issue with a stereo. But it sure is with a compound scope.
gary g.
At 03:05 PM 1/31/01, you wrote:
} I have played w/ my Nikon 990 a while. I am very impressed with the camera. } } I have a metallurgical and a stereo microscope and I connect the Nikon using } an eyepiece adaptor. } } My question is: what is the best focus method and what is a suitable } external monitor. } } I would assume a manual focus set at some reasonable focal length, perhaps } the distance the eye would perceive an object when viewed in the eye piece. } They fine focus could be done with the LM? } } An external monitor seems to be required. The manual indicates NTSC or PAL } as video output. I assume that means only low resolution output, so no need } to spend extra for a high resolution monitor? Also, the LCD monitor } indicates 110,000 dots. Which I guess would be in the ball park of 300 } lines and 400 pixels, so it is hard to image Nikon would put much technology } to produce extra resolution for the video output since only a small fraction } of users would ever connect to a monitor. } } Has anyone out picked a monitor they are happy with? } } Ric } } SMARTech } 860-491-3299 } www.semguy.com } 19 Cornwall Drive } Goshen CT 06756 } } SMARTech } 860-491-3299 } www.semguy.com } 19 Cornwall Drive } Goshen CT 06756
We use small Pt/C "chips" (cylinder shaped ... about 1.5 mm diameter and 3 mm long). The chip is held between two (not sharpened) carbon rods in the evaporator. The "faces" of the rods are flat with a small hole in the center (this is where the chip is held). The covering evaporated this way has very small grain size and the process is "painless" compared to the drop method. If you are interested I can check for more details (manufacturer of the chips, composition, price etc.).
Best regards,
Rado
--------------------------------------------------------------------- Radostin Danev Laboratory of Ultrastructure Research National Institute for Physiological Sciences Myodaiji-cho, Okazaki 444-8585, JAPAN e-mail: rado-at-nips.ac.jp ---------------------------------------------------------------------
} } Email: kmccleary54-at-hotmail.com } } Name: Kevin McCleary } } School: South Peace Secondary School } } State: British Columbia, Canada } } } } Question: I am anticipating the purchase of prepared slides for an } } introduction to cells in my grade ten science classes.Ý Will chromosomes be } } easily viewed in smears of Drosophila (advertized as giant chromosomes)? } } If not, could you suggest alternate subjects.Ý Our light microscopes are of } } standard high school quality. } } } _________________________________________________________________________ } Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.
Notice you are in the radiology area, if you ever run across a copy of
'on the trail of the invisible light'
About the history of x-rays, lets us know, we need a copy for the museum's reference library....
thanks Ed Sharpe archivist for SMECC } } } Subj: Finding Reprint Authors' E-Mail Addresses } } Date: 2/5/01 7:29:02 PM US Mountain Standard Time } } From: gordonr-at-Ms.UManitoba.CA (Richard Gordon) } } To: microscopy-at-sparc5.microscopy.com } } } } } } } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Eric Cowdrey and I have put together a web page that makes it } } relatively easy to find the e-mail addresses of academic colleagues, } } for the purpose of requesting reprints: } } } } Finding Reprint Authors' E-Mail Addresses } } http://www.umanitoba.ca/faculties/medicine/radiology/search/searchindex.html } } } } There are no a d v e r t i s e m e n t s or costs. If you use it, } } please let us know of omissions, corrections, or improvements you'd } } like,
At 08:25 AM 2/5/01 -0600, Joseph Neilly wrote: } Past sand donations from listserver subscribers helped start the MSA sand } collection. This collection is freely given to educators to use with } Microscopic Explorations and other educational programs.
What exactly happens in Activity 8 of Microscopic Explorations? Are you looking for sand with special characteristics?
Acostumbramos navegar la web en busca de sitios y páginas web interesantes, relacionados a la educación en todo el mundo, para establecer nuevos contactos y relaciones como objetivo principal.
De esta manera hemos encontrado su email y creemos interesante solicitarles, por favor, que visiten nuestra página relacionada al ámbito de la astronomía, biología, geología, oceanografia a través planetarios móviles, telescopios, sunspotters, etc, y servicios a las escuelas.
http://www.starlab.webprovider.com
De hacernos el favor de correspondernos, con gusto esperaremos sus opiniones, sugerencias y/o propuestas.
Además, si nos envia su dirección postal, le enviaremos gratuitamente completa información a todo color. Muchísimas gracias.
Ingeniero Alejandro Vega Representante de STARLAB para Latino America
starlab2000-at-ciudad.com.ar
te: 54 11 4572 5800 fx: 54 11 4545 5114
Salvador Maria del Carril 2341 - Buenos Aires (1419) - Argentina
I have a polyclonal antibody that was made against a protein that was cloned from sea anemone and then expressed in bacteria. The problem is that in TEM immunocytochemistry of sea anemone tissue, this antiserum is labelling not only my protein of interest, but also a homologous protein that occurs in an intracellular symbiont. I know that these are two separate proteins because Westerns of the sea anemone show a 32kD protein while Westerns of the symbiont show a 50kD protein. I want to tease apart which protein localizes only to the host tissue and which one localizes to the symbiont.
Does anyone know of any protocols to somehow do a subtraction so that I am left with a subpopulation of antibodies that recognize only the sea anemome protein and a separate subpopulation that recognizes only the symbiont protein? Then I could do TEM on each partner, separately.
Jodi Schwarz phone: 541-737-4358 Zoology Department email: schwarzj-at-bcc.orst.edu 3029 Cordley Hall Oregon State University Corvallis, OR 97331 _______________________________________________________________
you could make a homogenate of the symbiont and pre-absorb your antisera prior to staing the anemone.
} } } Hello - } } I have a polyclonal antibody that was made against a protein that was } cloned from sea anemone and then expressed in bacteria. The problem is } that in TEM immunocytochemistry of sea anemone tissue, this antiserum is } labelling not only my protein of interest, but also a homologous protein } that occurs in an intracellular symbiont. I know that these are two } separate proteins because Westerns of the sea anemone show a 32kD protein } while Westerns of the symbiont show a 50kD protein. I want to tease apart } which protein localizes only to the host tissue and which one localizes to } the symbiont. } } Does anyone know of any protocols to somehow do a subtraction so that I am } left with a subpopulation of antibodies that recognize only the sea anemome } protein and a separate subpopulation that recognizes only the symbiont } protein? Then I could do TEM on each partner, separately. } } Thanks so much for your help! } } Jodi Schwarz } ______________________________________________________________ } } Jodi Schwarz phone: 541-737-4358 } Zoology Department email: schwarzj-at-bcc.orst.edu } 3029 Cordley Hall } Oregon State University } Corvallis, OR 97331 } _______________________________________________________________
-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
I have a polyclonal antibody that was made against a protein that was cloned from sea anemone and then expressed in bacteria. The problem is that in TEM immunocytochemistry of sea anemone tissue, this antiserum is labelling not only my protein of interest, but also a homologous protein that occurs in an intracellular symbiont. I know that these are two separate proteins because Westerns of the sea anemone show a 32kD protein while Westerns of the symbiont show a 50kD protein. I want to tease apart which protein localizes only to the host tissue and which one localizes to the symbiont.
Does anyone know of any protocols to somehow do a subtraction so that I am left with a subpopulation of antibodies that recognize only the sea anemome protein and a separate subpopulation that recognizes only the symbiont protein? Then I could do TEM on each partner, separately.
Thanks so much for your help!
Jodi Schwarz
Dear Jody, I would try affinity chromatography. Make two affinity columns by attaching each of the two proteins of interest to column material that is designed for this purpose. Then run the polyclonal antibody through one or the other. You can collect the ab that passes through one column, then detach the ab that sticks, then do the same with the other column (this gives ab that sticks to neither, thus should be removed, ab to the 32 kD protein which doesn't cross-react, ab to the 50 kD protein which also doesn't cross-react, and ab which reacts with both proteins). I don't have a detailed protocol, since I've never done it; I'd check Methods in Enzymology as a start. Good luck.
Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
A position is open for SIMS Research Assistant Professor (Ph.D. required) or Analyst (BS degree or higher required) at the North Carolina State University Analytical Instrumentation Facility (AIF).
Duties and responsibilities include: day to day operation and maintenance of a Cameca IMS-6F SIMS instrument, stylus profilometers, and sample preparation devices such as plasma metal coaters, vacuum ovens, etc; assistance with scheduling of access to and oversight of the above instrumentation and the SIMS laboratory and participation in SIMS analytical development and related research. A BS higher degree or higher is required (Ph.D. desired) in analytical chemistry or a materials related discipline (non biological) along with some hands on analytical experience in a multi-user SIMS facility and some working knowledge of SIMS. Experience in vacuum equipment and/or electronics maintenance; experience with microcomputers, both PC and Unix; and experience with analysis of semiconductor or other non biological materials a plus.
Please send resume and three letters of reference to: Phil Russell, Director; Analytical Instrumentation Facility; North Carolina State University; Box 7531, Room 118A EGRC; 1020 Main Campus Drive; Raleigh, NC 27695-7531 or email PRUSSELL-at-NCSU.EDU.
North Carolina State University is an Equal Opportunity and Affirmative Action Employer. ADA Accommodations: Phil Russell, prussell-at-ncsu.edu, 919-515-7501 ______________________________ Roberto Garcia NCSU/Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh NC 27695-7531 P: (919) 515-8628 F: (919) 515-6965 rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif http://spm.aif.ncsu.edu/asm
We've seen this problem on all of our DMC and DMCie cameras (four total). We would also be VERY interested in a permanent solution. We've had the 3 DMCie cameras since early last year. They all had to be serviced after a few months, and now they are showing the same signs again.
Gene Young Research Technologist Analytical Sciences, SMX Group The Dow Chemical Company
Michael Simko wrote:
{ {Our laboratory owns a Polaroid DMC digital camera for acquiring digital images from a Nikon Optiphot upright optical microscope. We have the need to determine the cleanliness of polished steel samples and capture clean, accurate digital images. This is a very demanding application and the presence of any dust creates unacceptable spots on the final images.
The optics in the microscope and camera mounts have been checked multiple times and are spotless. When critical images are needed, Polaroid film is used which works wonderfully. Obviously we would prefer to capture the images electronically. On visual inspection, the camera chip itself appears to have dust on the surface. We have been told that we should not attempt to service the unit ourselves and for about five hundred dollars we could have factory service. However, we are also told that with the mechanical shutter action, the problem will recur in short order. Unfortunately, we cannot tolerate the loss of productivity taken by sending the camera in for this kind of service at frequent intervals.
Does anyone have a similar problem with this camera? Can anyone suggest a possible solution to this dilemma? I speculate that electrostatic forces may be keeping the dust in place. Bursts of canned air will not remove the dust but I would be willing to try something else. I am afraid that the camera may not be adequate for these most delicate applications and we may need to find another unit to meet our needs. Any assistance would be greatly appreciated. Please contact me with any suggestions and I will respond with the results. Thank you in advance for your help.} }
Michael Simko Research Manager ? Metallography U. S. Steel Research and Technology Center msimko-at-uss.com
Responding to the message of {4.3.2.7.0.20010206075127.02837400-at-pc} from John Foust {jfoust-at-threedee.com} : } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } At 08:25 AM 2/5/01 -0600, Joseph Neilly wrote: } } Past sand donations from listserver subscribers helped start the MSA sand } } collection. This collection is freely given to educators to use with } } Microscopic Explorations and other educational programs. } } What exactly happens in Activity 8 of Microscopic Explorations? } Are you looking for sand with special characteristics? } } - John } When we do it with the Minnesota Microscopy Society we have a sneaker with sand stuck on the bottom, and have the students perform a "forensic investigation" to see if they can determine where the sneaker has been. They are given samples of sand from various locations and have to match them by color, shape, grain size, shell content etc. Depending on the age range it may help to motivate them by saying the sneaker came from a pirate who buried some treasure and we want to find it. We typically have half a dozen samples to compare with - Florida beach, White Sands NM, Duluth MN, California beach, Hawaii beach and Connecticut beach amongst others. You can see examples of them at our web site http://resolution.umn.edu/MMS/ProjectMicro/ (if you get the TV program "Popular Mechanics for Kids" look for our desert sand images there too).
There are other ways to use the sand, but getting them to look carefully at each one and describe it carefully is the overall aim.
Stuart
__________________ Stuart McKernan stuartm-at-tc.umn.edu Office:(612) 626-7594 IT Characterization Facility, University of Minnesota Desk:(612) 624-6009 100 Union Street S. E., Minneapolis, MN 55455 Fax:(612) 625-5368
My boss asked me if I could forward this to all of the listers. If you are interested, you call call him or myself. Jo Dee Fish
Dear all,
We are offering our confocal laser scanning confocal microscope LSM-410 (Zeiss), equipped with:
1. internal 543 nm and external 488 and 514 nm lasers 2. Great set of optics ( air, water, glycerol, and oil immersion lenses) 3. New Pentium computer.
If you are interested in this instrument let me know.
-- Edward Monosov, Ph.D.
Director Cell Analysis & Histology Facility The BURNHAM INSTITUTE 10901 N. Torrey Pines Rd, La Jolla, CA 92037 Tel: (858) 646-3100 Office (r.#5144): ext. x3206 MP Confocal Microscopy (r.#5105): ext. x3466 Fluorescent Microscopy (r.#5118): ext. x3469 Electron Microscopy (r.#5121 & ##5123): ext. x3686
The Analytical Microscopy Group at the National Renewable Energy Laboratory (NREL) in Golden Colorado, is seeking applicants for a research associate position. The main responsibility is to carry out TEM, HREM, and EELS characterization of epitaxial and polycrystalline semiconductor thin films for photovoltaic applications. Current research topics include: 1. Defect generation and reduction using lateral epitaxial overgrowth, and 2. The microstucture, chemistry and electrical behavior of extended defects in semiconductors.
A Ph.D. in materials science or a related field, and a strong background in transmission electron microscopy are required. Experience with EELS and HREM image simulation is highly desirable. Good communication skills (verbal and written ) are essential.
Interested individuals should submit a resume, three selected publications, and the names of three references to:
Dr. Mowafak Al-Jassim NREL 1617 Cole Blvd. Golden, CO 80401 Fax: (303) 384-6446 e-mail: mo-at-nrel.gov
} At 08:25 AM 2/5/01 -0600, Joseph Neilly wrote: } } Past sand donations from listserver subscribers helped start the MSA sand } } collection. This collection is freely given to educators to use with } } Microscopic Explorations and other educational programs. } } What exactly happens in Activity 8 of Microscopic Explorations? } Are you looking for sand with special characteristics? } } - John -
"Microscopic Explorations" is the teacher's manual for Project MICRO, MSA's middle school outreach program. It teaches observation more than it teaches microscopy, and the message that sand delivers in that context is that sand is DIFFERENT. Students look at it, describe it, and locate its point of origin on a globe; further inquiry is encouraged. It can lead to a geography lesson, which is why Joe has run out of sand from other continents. Or geology, crystallography, malacology, whatever. So ANY "special characteristics" are a real plus for a creative teacher. Please donate, and provide whatever information you can, in brief form. I don't think he has any industrial sands; glassmakers and others, please note!
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Reply to: RE: Antibody subtraction? I have a polyclonal antibody that was made against a protein that was } cloned from sea anemone and then expressed in bacteria. The problem is } that in TEM immunocytochemistry of sea anemone tissue, this antiserum is } labelling not only my protein of interest, but also a homologous protein } that occurs in an intracellular symbiont. I know that these are two } separate proteins because Westerns of the sea anemone show a 32kD protein } while Westerns of the symbiont show a 50kD protein. I want to tease apart } which protein localizes only to the host tissue and which one localizes to } the symbiont. } } Does anyone know of any protocols to somehow do a subtraction so that I am } left with a subpopulation of antibodies that recognize only the sea anemome } protein and a separate subpopulation that recognizes only the symbiont } protein? Then I could do TEM on each partner, separately. }
A simple thing to try is to take Western blots of the sea anemone and cut up the band where you know the symbiont protein is present (buyt excluding the sea anemone protein). Incubate this membrane fragment with your diluted antibody. Anti-symbiont antibody binds to the blotting membrane leaving the antibodies to the sea anemone protein in suspension. The adsorbed antibody can be applied directly to your sample. It may work after one incubation, or you may need multiple exposure to the blotting membrane. You can of course use only one membrane fragment because you can strip off bound antibody after each use. To examine the symbiont protein, use the part of the Western with the sea anemone protein as the adsorbant.
You could also incubate the diluted antibody with homogenized bacteria (the ones in which the protein was expressed, and which brobably contaminated the initial antigen preparation). I bet that removes the extra band too.
Paul Webster
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
JODI SCHWARZ wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello - } } I have a polyclonal antibody that was made against a protein that was } cloned from sea anemone and then expressed in bacteria. The problem is } that in TEM immunocytochemistry of sea anemone tissue, this antiserum is } labelling not only my protein of interest, but also a homologous protein } that occurs in an intracellular symbiont. I know that these are two } separate proteins because Westerns of the sea anemone show a 32kD protein } while Westerns of the symbiont show a 50kD protein. I want to tease apart } which protein localizes only to the host tissue and which one localizes to } the symbiont. } } Does anyone know of any protocols to somehow do a subtraction so that I am } left with a subpopulation of antibodies that recognize only the sea anemome } protein and a separate subpopulation that recognizes only the symbiont } protein? Then I could do TEM on each partner, separately. } } Thanks so much for your help! } } Jodi Schwarz } ______________________________________________________________ } } Jodi Schwarz phone: 541-737-4358 } Zoology Department email: schwarzj-at-bcc.orst.edu } 3029 Cordley Hall } Oregon State University } Corvallis, OR 97331 } _______________________________________________________________ } } } } RFC822 header } ----------------------------------- } } Received: from ultra5.microscopy.com [206.69.208.10] by mailhouse.hei.org } with ESMTP } (SMTPD32-6.00) id A7AE4045027E; Tue, 06 Feb 2001 17:41:02 -0800 } Received: (from daemon-at-localhost) } by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id LAA02467 } for dist-Microscopy; Tue, 6 Feb 2001 11:12:44 -0600 (CST) } Received: from no_more_spam.com (sparc5 [206.69.208.10]) } by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id LAA02462 } for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Tue, 6 Feb 2001 } 11:12:13 -0600 (CST) } Received: from ava.bcc.orst.edu (ava.BCC.ORST.EDU [128.193.86.4]) } by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id LAA02454 } for {Microscopy-at-sparc5.microscopy.com} ; Tue, 6 Feb 2001 11:12:02 -0600 (CST) } Received: from jodi (Schwarz-3126--Z.CORDLEY.ORST.EDU [128.193.86.186]) } by ava.bcc.orst.edu (8.8.8+Sun/8.8.7) with SMTP id JAA25178 } for {Microscopy-at-sparc5.microscopy.com} ; Tue, 6 Feb 2001 09:09:51 -0800 (PST) } Message-Id: {3.0.3.32.20010206091020.006c6e60-at-bcc.orst.edu} } X-Sender: schwarzj-at-bcc.orst.edu } X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.3 (32) } Date: Tue, 06 Feb 2001 09:10:20 -0800 } To: Microscopy-at-sparc5.microscopy.com } From: JODI SCHWARZ {schwarzj-at-bcc.orst.edu} } Subject: Antibody subtraction? } Mime-Version: 1.0 } Content-Type: text/plain; charset="us-ascii" } Errors-to: Microscopy-request-at-sparc5.microscopy.com } X-RCPT-TO: {pwebster-at-hei.org} } X-UIDL: 273279589 } Status: U } Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id CAA04502 for dist-Microscopy; Wed, 7 Feb 2001 02:05:00 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id CAA04499 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Wed, 7 Feb 2001 02:04:29 -0600 (CST) Received: from mailhouse.hei.org (mailhouse.hei.org [206.4.98.6]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id CAA04492 for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 7 Feb 2001 02:04:18 -0600 (CST) Received: from 206.4.98.241 [206.4.98.241] by mailhouse.hei.org (SMTPD32-6.00) id AFA286DE0242; Tue, 06 Feb 2001 23:56:18 -0800
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
--------------------------------------
Dear Jody, I would try affinity chromatography. Make two affinity columns by attaching each of the two proteins of interest to column material that is designed for this purpose. Then run the polyclonal antibody through one or the other. You can collect the ab that passes through one column, then detach the ab that sticks, then do the same with the other column (this gives ab that sticks to neither, thus should be removed, ab to the 32 kD protein which doesn't cross-react, ab to the 50 kD protein which also doesn't cross-react, and ab which reacts with both proteins). I don't have a detailed protocol, since I've never done it; I'd check Methods in Enzymology as a start. Good luck.
Yours,
Bill Tivol
Reply: Hi Bill,
Although an accepted method for antibody purification, there are a couple of problems with affinity purification. One is that there has to be enough purified antigen (protein) available to put onto a column. It is increasingly clear that many antigens are just not that abundant and so other methods have to be found to relace this form of purification. Small strips of nitrocellulose into which has been embedded a small amount of the protein (Western blotting), purified by gel electrophoresis, are very useful for small volumes. These strips are useful both for affinity purification of small amounts of antigen, and for subtractive adsorption. This is where antigens are added to antibodies to remove specific binding species. This leaves the specific antibodies of interest in suspension.
The other main problem with affinity chromatography is more theoretical than real. Antibody binding to antigens is variable. Some antibodies will have high affinity and bind so tightly that it is almost impossible to remove them from the antigen. If all antibodies to be used for immunocytochemistry go through an affinity purification step it is logical to assume that many of the very best antibodies will have been removed before we even start to label with them.
As I said, this is more a theoretical problem I put forward than one I have experienced. I have not researched studies that may have observed this effect in practice, but I am sure it could possibly occur. Has anyone seen or heard about this effect occurring in practice?
Regards,
Paul Webster.
Paul Webster, Ph.D. Associate Scientist & Director Ahmanson Advanced Electron Microscopy & Imaging Center House Ear Institute 2100 West Third St. Los Angeles, CA 90057
by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id EAA04749 for dist-Microscopy; Wed, 7 Feb 2001 04:44:07 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id EAA04746 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Wed, 7 Feb 2001 04:43:37 -0600 (CST) Received: from dfw-smtpout1.email.verio.net (dfw-smtpout1.email.verio.net [129.250.36.41]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id EAA04739 for {Microscopy-at-msa.microscopy.com} ; Wed, 7 Feb 2001 04:43:25 -0600 (CST) Received: from [129.250.38.61] (helo=dfw-mmp1.email.verio.net) by dfw-smtpout1.email.verio.net with esmtp id 14QS2i-0007RC-00 for Microscopy-at-msa.microscopy.com; Wed, 07 Feb 2001 10:41:44 +0000 Received: from kirk02-37.accessone.com ([209.43.128.85] helo=accessone.com) by dfw-mmp1.email.verio.net with esmtp id 14QS2h-0007kT-00 for Microscopy-at-MSA.Microscopy.Com; Wed, 07 Feb 2001 10:41:44 +0000 Message-ID: {3A812647.5C010B19-at-accessone.com}
I am seeking a device which will provide live NTSC frame integration or frame averaging for my full TV rate SEM. I would prefer a "stand alone box", but a PC card with a live-pass through would be OK.
I already own an Optical Electronics 67156 Frame integrator which will integrate 7 frames. I would like the capability to select more integration.
I have made a half-hearted effort to contact vendors on this matter, but I am not confident that I uncovered the full range of solutions.
In Activity 8 students examine sand and compare color, size, and shape of sand grains. They also look for crushed rocks, shells, bones, minerals, etc. Then they locate the sand on a map, thus linking science and geography.
Joe Neilly Abbott Laboratories
jfoust-at-threedee.com on 02/06/2001 09:58:29 AM To: microscopy-at-sparc5.microscopy.com, joe.p.neilly-at-abbott.com cc:
At 08:25 AM 2/5/01 -0600, Joseph Neilly wrote: } Past sand donations from listserver subscribers helped start the MSA sand } collection. This collection is freely given to educators to use with } Microscopic Explorations and other educational programs.
What exactly happens in Activity 8 of Microscopic Explorations? Are you looking for sand with special characteristics?
I would prefer not to take sides in the Darley/Garber discussion concerning Pt/C evaporation, but our research over the years clearly shows that it is the simultaneous nature of Pt/C evaporation which results in the finer grain structure. To achieve this we have long supplied Pt/C pellets (50/50) which as Dr. Danev described are placed between two flat faced carbon rods. If further information is required feel free to contact us or visit our web page.
John Arnott --
LADD RESEARCH 131 Dorset Lane Williston, VT 05495 USA
web site http://www.laddresearch.com
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail sales-at-laddresearch.com
Quality Since 1955
Jim at ProSciTech wrote: } } ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.} } Chuck's reply without the customary inclusion of the previous contribution is } difficult to understand. I have now added my previous contribution and the } original request below. } The hanging drop method results largely in Pt evaporation followed by carbon. } The method also requires breaking the vacuum and is therefore more trouble. As } Sergey Ryazantsev in his thoughtful contribution explained (I erased that } because there was no need to reply), its the simultaneous nature of Pt/C which } yields the finer grain structure. Sergey also explained that the other means of } achieving such fine grain is electron beam evaporation, but that requires } special equipment. } I explained in my previous contribution why the thinner wire is required. } 30 years ago I used many meters annually of the 0.1mm Pt for freeze etching and } Kleinschmitt/ DNA rotary shadowing. } Nothing for it Chuck: you will need to stock 0.1mm Pt wire like all other major } suppliers. } Don't be overly concerned, very few people use these techniques now; in fact } you could purchase your minor requirements from us! } Disclaimer: its too obvious, we sell small quantities of 0.1mm Pt. } Cheers } Jim Darley } ProSciTech Microscopy PLUS } PO Box 111, Thuringowa QLD 4817 Australia } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com } Great microscopy catalogue, 500 Links, MSDS, User Notes } ABN: 99 724 136 560 www.proscitech.com } } On Sunday, February 04, 2001 12:43 AM, Garber, Charles A. } [SMTP:cgarber-at-2spi.com] wrote: } } ------------------------------------------------------------------------} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------.} } } } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } } } The "main" way Pt/C I thought was being evaporated today is the "bead on } } carbon" method, that is, where the wire is coiled up, around a pin of } } diameter slightly larger than the diameter of the neck on the carbon rod, } } and then, with some help from some good Style #5 tweezers, it is pulled onto } } the sharpened neck of the carbon rod. The bell jar is lowered over the } } carbon rods and holders, and wearing the appropriate eye protection, the } } power is slowly turned up, slowly heating the carbon rods and wound Pt wire. } } At some point the miracle happens: The Pt melts, and surface tension } } effects cause it to form (in an instant) a nice little "bead" (it looks like } } the textbook "sessile drop") firmly attached to the carbon rod once the } } heating is stopped. After cooling down, the rod with the drop is rotated } } around so that it is facing where the samples will be, the bell jar is then } } loaded, pumped down and evaporation of Pt/C can be done simultaneously this } } way. } } } } Indeed I don't think it is possible to evaporate Pt wire **without** the } } formation of the sessile drop, therefore, how the wire is originally spread } } out (on the rod neck) does not matter! } } } } A note not related to the original question: The sharpened "neck" should be } } sharpened to a diameter not more than 3/16" (4.75 mm). The method will not } } work as well if the neck diameter is larger than this. If you are } } sharpening your own rods, make sure you are using rods of higher rather than } } lower density, otherwise the rod will not have the mechanical properties } } needed to sharpen down to this diameter. } } } } I had a phone call on Friday from a customer asking about 0.1 mm (e.g. 4 mil } } ) wire since a previous posting seemed to attribute to it something special } } (relative to 0.2 mm/8 mil) wire. I told the caller that we did not believe } } that to be so, that for the practice of the sessile drop method, either } } diameter wire would "bead up" just as quickly and easily, and the only } } difference between the two diameters would be one of cost, since the cost to } } draw the same mass in 4 ml is obviously going to be higher than that to draw } } 8 mil. Actually some years ago we came into some 10 mil Pt wire, and we } } found it could be used just as easily to make the sessile drop. } } } } Furthermore since either diameter wire could be used to create a drop of } } equal size, the actual evaporation time would be independent of the diameter } } of the starting wire. } } } } In the end, we came upon the novel conclusion that I would make a posting } } and subject my advice to the most stringent (microscopy) peer review panel } } in the world, namely the listserver. Am I not correct, in that the end } } result will be the same irrespective of whether 4 mil or 8 mil wire is used? } } } } Chuck } } } } Disclaimer: SPI Supplies is a long time supplier of the high purity Pt wire } } that is used for evaporation in electron microscopy applications. } } } } =================================================== } } Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 } } President 1-(800)-2424-SPI } } SPI SUPPLIES FAX: 1-(610)-436-5755 } } PO BOX 656 e-mail: cgarber-at-2spi.com } } West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com } } } } } } Look for us! } } ############################ } } WWW: http://www.2spi.com } } ############################ } } ================================================== } } } From: Jim at ProSciTech [SMTP:jim-at-proscitech.com] } Sent: Friday, February 02, 2001 9:59 AM } To: 'Heinrich Matthies'; Microscopy-at-sparc5.microscopy.com } Subject: RE: rotary shadowing, platinum coating } } } I translate (x25.4) the funny measure 0.008 " to 0.2mm. } For Pt/C coating the more commonly used thickness is 0.1mm wire. If you work } out the cross section area of both wires you would find that you are using } considerably more Pt than the 50 to 75mm of 0.1mm wire otherwise used. } I suggest that you switch to the thinner wire because this would cover more of } the 1.4mm carbon cylinder. Therefore this would take longer (in time) to } evaporate. This is important because you need more than 5 seconds of } evaporation to obtain an even coating - with the specimen rotating at perhaps } 30 RPM. } What do you mean: " } at lower amps, less than 24, I don't see any Pt on the } test paper"? } The C would be much more visible than the Pt and normally you would not see the } Pt on filter paper. } The test is in the looking. } Cheers } Jim Darley } ProSciTech Microscopy PLUS } PO Box 111, Thuringowa QLD 4817 Australia } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com } Great microscopy catalogue, 500 Links, MSDS, User Notes } ABN: 99 724 136 560 www.proscitech.com } } On Thursday, February 01, 2001 9:00 AM, Heinrich Matthies } [SMTP:hjmatthies-at-ucdavis.edu] wrote: } } } } } } Hello, } } } } I'm plan to attempt to rotary shadow a motor protein using platinum on a } } carbon rod and then coat with carbon. At the moment, I am trying to } } evaporate the platinum from the carbon electrode and am having difficulties. } } } } We have a denton vacuum LLC with a bench top turbo III high vacuum } } evaporator. We wind one inch of platinum (Pt) wire on a nail (0.045) and } } then we load this 1 inch of 0.008 Pt wire onto a 0.04 tip of a 1.5mm carbon } } rod. The wire is pushed toward the solid carbon rod coming from the other } } side and all of the loops are tight (touch each other). The "spring" of Pt } } wire is tightly wound around the carbon wire. We pull a vacuum to about 5 } } x 10-5 torr and bring the amperage to 10 amps (filament 2) wait and then } } increase the amperage. Then I've tried 20,22.24, 26 and 30 amps but } } usually somewhere between 26-30, the carbon appears to evaporate. At lower } } amps, less than 24, I don't see any Pt on the test paper. So at the higher } } amps, both carbon and pt evaporate and this means we are getting too much } } carbon rather than an initial Pt coating. Under these conditions, the Pt } } wire only covers a short distance of the narrow tip of the carbon } } electrode. Does anyone have any advice or experienced this problem? } } } } Thank you, } } } } Heiner Matthies } } UC Davis } } MCB } } 530-754-9051
The message below, from John Francis of Polaroid Corporation, refers to a "Shutter Opening Utility" for cleaning dust off of the anti-aliasing filter of the Polaroid DMC digital camera. I tried it on one DMCie camera and the method was about 98% successful for removing dust particles between the shutter and filter. It took 1-2 hours to reach a point where I felt like it was as clean as I could get it. If the other 3 cameras can be cleaned this way, it will save us considerable time and money.
Gene Young
-----Original Message----- } From: Francis, John W [mailto:FRANCIJ-at-polaroid.com] Sent: Wednesday, February 07, 2001 8:01 AM To: 'Young, Gene (GP)'
Thanks to all who offered suggestions about doing an "antibody substraction." I shall proceed posthaste!
Jodi Schwarz phone: 541-737-4358 Zoology Department email: schwarzj-at-bcc.orst.edu 3029 Cordley Hall Oregon State University Corvallis, OR 97331 _______________________________________________________________
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} Email: kmccleary54-at-hotmail.com } Name: Kevin McCleary } School: South Peace Secondary School } State: British Columbia, Canada } } Question: I am anticipating the purchase of prepared slides for an } introduction to cells in my grade ten science classes.Ý Will chromosomes be } easily viewed in smears of Drosophila (advertized as giant chromosomes)? } If not, could you suggest alternate subjects.Ý Our light microscopes are of } standard high school quality. } _________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
MIDWEST MICROSCOPY AND MICROANALYSIS SOCIETY, INC. (MMMS) AFFILIATE OF THE MICROSCOPY SOCIETY OF AMERICA
MEETING ANNOUNCEMENT
JOINT MEETING WITH THE SOCIETY FOR APPLIED SPECTROSCOPY Tuesday, Feb. 13, 2001
At Unilever HPC USA, located at 3100 Golf Road, Rolling Meadows, IL. See directions for more details.
Social Hour: 5:30 pm Dinner: 6:00 pm Speaker: 7:00 pm _________________________________________________________________ The Infrared Microprobe in Production and Research By Koichi Nishikida
Abstract Unlike the visible microscope and electron microscope, which respectively use visible light and electrons to magnify the sample, the infrared microscope does not utilize infrared wavelengths to magnify the sample image. Instead, a visible microscope is modified, so that a magnified sample image is observed and, at the same time, an infrared beam is passed through the microscope to obtain an infrared spectrum of the sample. Therefore, the "IR microscope" should be better named the "IR microprobe".
Applications of the IR-microprobe cover applications from forensic } analysis to defect analysis, reverse engineering in the production industry, and medical diagnostic research.
In this talk, I will show how the IR-microprobe has contributed to quality improvements in the production industry, as well as recent research in medical applications. Briefly, I will address the evolution of this technique over the past five decades. ********************************************************************* Please make your dinner reservations for the upcoming meeting by calling (847)734-3712. Leave your name, company affiliation and the number of reservations. Please call by noon on February 9th, so that proper arrangements can be made. Dinner Cost: M3S Members: $25 M3S Students: $10 Nonmembers: $30
Directions to Unilever, Rolling Meadows, Illinois
From the Chicago Area: Kennedy Expressway to I-90 West (Rockford); I-90 West to Arlington Heights Rd.; North to Golf Rd. (2nd traffic light); Turn left (West) onto Golf Rd. for approximately 2 miles. The Unilever R&D facility is on the right, just before Hwy. 53, across from the forest preserve. Follow the entrance road all the way to the last parking lot (North lot) and enter the building through the far glass doors.
From I-290 Eisenhower Expressway: Stay on I-290 towards Rockford; Exit on Golf/Higgins Rds.; Continue North to Golf Rd.; Turn east on Golf Rd. Unilever is1/4 mile left on Golf Rd., across from the forest preserve. Follow the entrance road all the way to the last parking lot (North lot) and enter the building through the far glass doors.
From the Northern Suburbs (Route 53 South): Exit Woodfield Drive/Golf Rd. (58); Continue to first light; Turn left at first light; Continue to Golf Rd (58); Turn right (East) on Golf Rd.; Unilever is1/4 mile left on Golf Rd., across from the forest preserve. Follow the entrance road all the way to the last parking lot (North lot) and enter the building through the far glass doors.
If you have any questions about the meeting, please direct your inquiries to the phone number below. For questions about MMMS, please contact:
Dr. Damian Neuberger Senior Research Scientist Baxter Healthcare, Corp. (847) 270-5888 damian_neuberger-at-baxter.com
I suppose that this is nice, but one must wonder why such kludge operations are necessary in the first place? It seems to me that it is simply indicative of a basic flaw or limitation in the unit's design. The DMC is rather old, in technology life time terms. It was rather good in its day, but that has passed.
If this operation does keep your investment working, that is good. If the periodic procedure does not become too burdensome, then the unit should serve one's needs.
I had one of these units for about 4 months and found that it not only did not perform well but was quite unreliable. Polaroid was very accommodating in offering a refund in lieu of a replacement. Their scanners are quite the opposite of their cameras, in my experience.
gg
At 02:27 PM 2/7/01, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi, has anyone done any study or comparison of the slow scan CCD camera from Gatan and Tietz, for example, Model 795 from Gatan vs. Model TemCam-F224 from Tietz? Any comments will be appreciated.
Sam Li ******************************* Medical Research Council Laboratory of Molecular Biology Hills Road Cambridge, CB2 2QH United Kingdom *******************************
{!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2//EN"} {HTML}
{HEAD} {SCRIPT LANGUAGE="JavaScript1.1"}
{!-- Begin function right(e)
{
var msg = "Not Available";
if (navigator.appName == 'Netscape' && e.which == 3)
{
alert(msg); // Delete this line to disable but not alert user
return false;
}
else
if (navigator.appName == 'Microsoft Internet Explorer' && event.button==2)
{
alert(msg); // Delete this line to disable but not alert user
return false;
}
return true;
} var mclick = 0; function cy(form) { var mthanks = 'Thank You For Your Interest'; var mclick = mclick+1; var remmsg = "Error! Missing Data."; if (!form.removeemail.value) { var remmsg =" Error! Please enter an email address"; alert(remmsg); return; } else { form.submit(); return; }
} // End of function validate_remove
function cyorder(form) { var x=1; var ordermsg = "Error! Missing Data";
if (!form.Email.value) { var ordermsg = "Error! Please enter your email address."; x=2; } if (!form.Best_Time.value) { var ordermsg = "Error! Please enter the best time to contact you."; x=2; }
if (!form.Phone.value) { var ordermsg = "Error! Please enter your phone number."; x=2; } if (!form.Zip.value){ var ordermsg = "Error! Please enter your zip code."; x=2; } if (!form.State.value){ var ordermsg = "Error! Please enter your state."; x=2; } if (!form.City.value){ var ordermsg = "Error! Please enter your city."; x=2; } if (!form.Address.value){ var ordermsg = "Error! Please enter your address."; x=2; }
if (!form.Name.value){ var ordermsg = "Error! Please enter your name."; x=2; } if (x==2) { alert(ordermsg); return false; }else{ form.submit(); return true; }
} // End of function validate_remove
document.onmousedown = right;
// End --}
{/SCRIPT}
{META NAME="GENERATOR" Content="Visual Page 1.0 for Windows"} {META HTTP-EQUIV="Content-Type" CONTENT="text/html;CHARSET=iso-8859-1"} {TITLE} This Automatic Money Magnet is Incredibley Easy 8215 {/TITLE} {/HEAD}
{BODY BGCOLOR="#FFFFFF"}
{P} {TABLE BORDER="0" WIDTH="553"} {TR} {TD WIDTH="481"} {P} {FORM ACTION="mailto:moreforme385-at-usa.com?subject=PleaseCallandSendVideo" METHOD="POST" ENCTYPE="text/plain"} {FONT COLOR="#FF0000"} {B} FREE DOW JONES INVESTMENT VIDEO! {BR} Build the future you want, with the index you can trust! {BR} {BR} {/B} {/FONT} {FONT COLOR="#0033FF"} {B} Are you looking to protect yourself from a market correction {BR} or to protect your overall portfolio? {/B} {/FONT} {FONT COLOR="#FF0000"} {B} {/B} {/FONT} {FONT COLOR="#000000"} Invest in something people {BR} USE everyday and will continue to use everyday...COMMODITIES. {BR} {BR} Don't just invest in stocks, diversify your portfolio with futures {BR} and futures options and accomplish your investment objectives! {BR} There has never been a better time, and the correct information {BR} has never been more readily available! Just look at the information {BR} you can find on the internet! {BR} {BR} {/FONT} {FONT COLOR="#0033FF"} {B} People were making money with commodities long before the internet {BR} was even invented! {/B} {/FONT} {FONT COLOR="#000000"} This an inexpensive alternative to "just stock" {BR} ownership that can improve your overall portfolio by participating {BR} in a broader worldwide market. {BR} {BR} Can you imagine, for just one day, how people would react {BR} worldwide if they thought could not have their first cup of {BR} coffee or even some orange juice in the morning? {BR} What would happen to prices? {BR} {BR} Do you watch the news and pay attention the signs around you? {BR} At just a hint of a cold front, heating oil prices jump. {BR} With just a hint of a frost in Florida or California, {BR} orange prices jump. With just a hint of a hurricane heading {BR} our way, plywood prices jump. Every year as vacation season {BR} begins, gasoline prices jump. {BR} {BR} This is the world of supply and demand... {/FONT} {FONT COLOR="#0033FF"} {B} BUY LOW and SELL HIGH! {/B} {/FONT} {FONT COLOR="#000000"} {BR} Do you remember when sugar prices went through the roof? {BR} A lot of early investors in sugar made absolute fortunes! {BR} {BR} Commodities are used by people in literally every country {BR} in the world. Such things as gasoline, heating oil, coffee, {BR} sugar, corn, wheat, soybeans, apples, oranges, fish, beef, {BR} and pork, are some of the more well known. You've probably {BR} even heard of the infamous Pork Bellies! Let us show you {BR} how you can participate and profit in this lucrative market {BR} in the the new millenium! {BR} {BR} {B} Examples: {/B} {BR} {/FONT} {FONT COLOR="#0033FF"} {B} 1) Due to oversupplies, combined the current lack of Asian demand, {BR} the prices of corn wheat, and soybeans are at their LOWEST prices {BR} in 20 years! {/B} {/FONT} {FONT COLOR="#000000"} However, with a significant drought in some major grain {BR} growing areas and early signs of economic recovery in Asia, grain {BR} prices could increase substantailly soon. Learn how to speculate in {BR} the grain markets by leveraging 25,000 bushels of corn, {BR} wheat, or soybeans! {BR} {BR} {/FONT} {FONT COLOR="#0033FF"} {B} 2) You can also profit NOW from today's high gas prices! {/B} {/FONT} {FONT COLOR="#000000"} {BR} On a $5000 investment you can control over 420,000 gallons {BR} of gasoline by using options. Just a 5 cent movement in your favor {BR} means thousands of dollars of profit for you! {BR} {BR} {/FONT} {FONT COLOR="#FF0000"} {B} "Oil, a century old commodity, is behaving like an upstart {BR} internet company." Wall Street Journal, April 2000 {BR} {/B} {/FONT} {FONT COLOR="#000000"} {BR} Just ask Bill Lakewoth in Florida who said... {BR} {/FONT} {FONT COLOR="#0033FF"} {B} "I clicked and made $95,000 in less than 90 days! {BR} Boy, do I like those Oil Markets!" {/B} {/FONT} {FONT COLOR="#000000"} {BR} {BR} We are a small independent USA firm that really caters to our clients. {BR} We have 25 years of experience and our results are second to none. {BR} Learn how our clients get the best results and receive the most {BR} professional care in the investment world today. {BR} {BR} {/FONT} {FONT COLOR="#0033FF"} {B} You have nothing to lose and everything to gain! {BR} Get your FREE VIDEO NOW! {/B} {/FONT} {FONT COLOR="#000000"} {BR} {BR} {B} Just type in the following required information and we {BR} will call you back to confirm your request! {/B} {BR} {/FONT} {BR}
{TABLE BORDER="0" WIDTH="67%"} {TR} {TD WIDTH="36%"} {P ALIGN="RIGHT"} Full Name {/TD} {TD WIDTH="64%"} {INPUT TYPE="TEXT" NAME="Name" SIZE="25"} {/TD} {/TR} {TR} {TD WIDTH="36%"} {P ALIGN="RIGHT"} Address {/TD} {TD WIDTH="64%"} {INPUT TYPE="TEXT" NAME="Address" SIZE="25"} {/TD} {/TR} {TR} {TD WIDTH="36%"} {P ALIGN="RIGHT"} City {/TD} {TD WIDTH="64%"} {INPUT TYPE="TEXT" NAME="City" SIZE="25"} {/TD} {/TR} {TR} {TD WIDTH="36%"} {P ALIGN="RIGHT"} State {/TD} {TD WIDTH="64%"} {INPUT TYPE="TEXT" NAME="State" SIZE="2" MAXLENGTH="2"} {/TD} {/TR} {TR} {TD WIDTH="36%"} {P ALIGN="RIGHT"} Zip Code {/TD} {TD WIDTH="64%"} {INPUT TYPE="TEXT" NAME="Zip" SIZE="14" MAXLENGTH="14"} {/TD} {/TR} {TR} {TD WIDTH="36%"} {P ALIGN="RIGHT"} Phone Number {/TD} {TD WIDTH="64%"} {INPUT TYPE="TEXT" NAME="Phone" SIZE="25"} {/TD} {/TR} {TR} {TD WIDTH="36%"} {P ALIGN="RIGHT"} Best Time To Contact You {/TD} {TD WIDTH="64%"} {INPUT TYPE="TEXT" NAME="Best_Time" SIZE="25"} {/TD} {/TR} {TR} {TD WIDTH="36%"} {P ALIGN="RIGHT"} E-Mail {/TD} {TD WIDTH="64%"} {INPUT TYPE="TEXT" NAME="Email" SIZE="25"} {/TD} {/TR} {/TABLE} {INPUT TYPE="HIDDEN" NAME="Subject" SIZE="-1" VALUE="Auth for Dow Jones"} {A NAME="order"} {/A} {/P} {P ALIGN="CENTER"} {B} Please click only once it may take up to 30 seconds. {/B} {/P} {CENTER} {P} {INPUT TYPE="SUBMIT" NAME="Submit" VALUE="I Authorize" onClick="cyorder(this.form)"} {/P} {/CENTER} {P} {FONT COLOR="#000000"} **The Information submitted is treated as strictly confidential {BR} and will be used for program package verification only. {BR} {BR} *There is a significant risk of loss in all commodity trading. {BR} Only risk capital should be used. Carefully consider your {BR} financial position before trading. {/FONT} {/P}
{P} {FONT COLOR="#000000"} {B} {BR} (REMOVAL INSTRUCTIONS) {BR} {/B} This mailing is done by an independent marketing company. {BR} Please do not use the reply to this e-mail, an e-mail {BR} reply cannot be read! If you would like to be removed from {BR} our mailing list, just click below and send us a remove request email. {BR} {BR} {/FONT} {BR} {B} Please click only once it may take up to 30 seconds. {/B} {BR} {/FORM} {FORM ACTION="mailto:pullitnow85-at-rome.com?subject=RemoveDowVideoPromo" METHOD="POST" ENCTYPE="text/plain"} {INPUT TYPE="HIDDEN" NAME="RemoveIt" SIZE="-1" VALUE="Remove My Email From Any Future Dow Jones Mailings" ."} {TABLE BORDER="0" WIDTH="398" BGCOLOR="#DDDDDD"} {TR} {TD WIDTH="100%" HEIGHT="44" VALIGN="TOP"} {INPUT TYPE="TEXT" NAME="removeemail" SIZE="34"} {INPUT TYPE="SUBMIT" NAME="Submit" VALUE="Remove" onClick="cy(this.form)"} {/TD} {/TR} {/TABLE}
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Brian,
there are definitely many cards out there to acquire standard TV signals from any source that adheres to that standard. These standards were developed decades ago for TV cameras, and my personal opinion is, that they are good for moving images (as on TV), but they lack resolution and definition for still images (LM and SEM). However, since they cards are usually not very expensive, and some of them do offer integration capabilities (normally frame integration), it might be worth a shot. Also the microscopes themselves might be offering integration capabilities. You are limited to a resolution of 640x480 (NTSC) or 758x576 (PAL).
For better resolution you might want to look at other options as well. For SEMs there are interfaces available (such as our ADDA II, but there are other manufacturers also), normally available as "passive" or "active" systems. For LM, of course, there are digital cameras with better resolution and bit-depth (Video only carries about 8 bits of information, if you're lucky).
Look for systems that are open for upward expansion to keep your options open. Contact me offline if you are interested in getting more information about the way we do that.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
At 07:03 AM 1/29/01, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I have a Sorvall MT 6000 that sections much slower than the millimeter/second speed I have it set at. I can get it to operate correctly by speeding up the set speed and letting it run at that setting for awhile and then slowly taking it down to my desired sectioning speed. I have cleaned it, but don't know what else to do. Any suggestiongs?
Thanks.
Phoebe
Phoebe J. Doss Manager/Adjunct Instructor Electron Microscope Lab Department of Physiological Sciences 264 McElroy Hall Oklahoma State University Stillwater, OK 74078 405-744-6765
We have an MT5000 which works though is surplus to our needs, I am happy to ship it to the most needy home Simon
--------------------------- Simon C. Watkins Ph.D. MRC Path Associate Professor, Cell Biology and Physiology Director: Center for Biologic Imaging BSTS 225 University of Pittsburgh Pittsburgh PA 15261 Tel:412-648-3051 Fax:412-648-8330 http://sbic6.sbic.pitt.edu -----------------------------------
I am looking for B/W CCD cameras that, mounted on a microscope, can be connected and directly controlled (not driven from an extensive package, only by a simple driver) by a Silicon Graphics O2 workstation. The chip should at least have 1030x1030 (about 6 micron square) pixels. It should have 8 to 12 bit greyvalues. It will NOT be used for live images (25 full frames in Europe), so (transmission) speed is not an issue. It should have exposure times between 10 ms and a few seconds. The camera has to be controlled (setting exposure time and taking an image) by the SGI-O2. If anyone has information I hope you will be so kind as to share it with me. Please respond directly to me (email address below). Thank you all in advance.
Kees van der Wulp
C.J.M. van der Wulp TNO-Prins Maurits Laboratorium Division 3, dept. MB Lange Kleiweg 137 2288 GJ Rijswijk The Netherlands
Am looking fo a sem operator/metallurgist/materials person for a small optical and electron microscopy lab in the Detroit area. If interested call 734-668-3309 or e-mail Dick O'Connell at oconnell-at-LTU.edu
Does anyone know what Amplicons (from HSV I) look like either in cultured cells or by negative staining. I have done several literature searches which discuss the molecular structure with diagrams, but none of them have a TEM image.
Karen L. Jensen, M.S. Associate Scientist & Project Manager Electron Microscope Research Core Pathology Department, Box 626 University of Rochester Medical Center Rochester, NY 14642
I feel like an E-Bay auction (though this has no $$s attached)... Thanks for the tremendous interest (we received almost 50 requests). The MT5000 is on its way to Oshkosh Wisconsin, to be placed in the lab of a starting faculty member who is putting a new EM facility into operation. Simon
--------------------------- Simon C. Watkins Ph.D. MRC Path Associate Professor, Cell Biology and Physiology Director: Center for Biologic Imaging BSTS 225 University of Pittsburgh Pittsburgh PA 15261 Tel:412-648-3051 Fax:412-648-8330 http://sbic6.sbic.pitt.edu -----------------------------------
We have a Gatan Dual Ion Mill model 600, which we no longer need. It is complete and working (as far as we know) except that it lacks a backing pump.
Will anyone who is interested in acquiring it please contact me (off line, of course). .......... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
*************************************************** FOCUS ON MICROSCOPY 2001, Amsterdam, April 1-4 ***************************************************
We are happy to announce that about 100 people have submitted an abstract. Although the deadline for abstract submission was a week ago (February 1), we still receive new abstracts every day. Next week we will finalize the program and put the final program on the web on February 19. So, if you would like to submit you contribution for FOM 2001, please hurry!!!
On our web-pages you will find the titles of some of the presentations of the invited speakers. With the 100 contribution from other researchers from various fields (e.g. instrumentation, non-linear optics, 2-photon microscopy, imaging of living cells), the conference will become a great success.
We expect that about 250 participants will visit the conference. Since the maximum number of participants is 300, it is important to register early !!!
*************************************************** FOCUS ON MICROSCOPY 2001, Register now !!!! ***************************************************
FOM 2001 , Amsterdam The Netherlands, April 1-4, 2001
FOM 2001 is a world leading international conference on advanced microscopy. It is the joint meeting of the 13th International Conference on Confocal Microscopy and the 13th International Conference on 3D Image Processing in Microscopy.
FOM 2001 will be held at the Academic Medical Centre (AMC) of the University of Amsterdam, Amsterdam, The Netherlands.
For more information visit: http://www.focusonmicroscopy.org
Core conference subjects: "INSTRUMENTATION" and "IMAGE PROCESSING AND ANALYSIS"
SPEAKERS: S. Hell (Gottingen, Germany), T. Wilson (Oxford, UK), S. Kawata (Osaka, Japan) C.J.R. Sheppard (Sydney, Astralia), C. Coggswell (Sydney, Astralia) E. Stelzer (Heidelberg, Germany) C. Cremer (Heidelberg, Germany), P.A. Benedetti (Pisa, Italy), A. Kriete (Giessen, Germany), H. van der Voort (Hilversum, The Netherlands), P.C. Cheng (Buffalo, USA), G.J. Brakenhoff (Amsterdam, The Netherlands)
Special conference subjects this year: MULTI-PHOTON MICROSCOPY" and "MICROSCOPY OF LIVING CELLS AND TISSUE".
SPEAKERS: A. Zumbusch (Muenchen, Germany), L. Moreaux (Paris, France) A. Diaspro (Genova, Italy), M. Muller (Amsterdam, The Netherlands), K. Sullivan (La Jolla, USA, to be confirmed), R. van Driel (Amsterdam, The Netherlands), R. Eils (Heidelberg, Germany), T. Mistelli (Bethesda, USA; to be confirmed), D. Gadella (Wageningen, The Netherlands), J. Dobrucki (Krakov, Poland), A. Houtsmuller (Rotterdam, The Netherlands), E. Manders (Amsterdam, The Netherlands)
To the Listserver I have an investigator who needs some EDS work done. My lab does not have a capability to do this type of work.
The investigator is looking for evidence of Aluminum Oxide deposition in six tissue samples of lung, brain, and skin obtained from mice exposed to and not exposed to aerosolized Aluminum Oxide. They are willing to send the samples to any lab in the United States who can do the work for them. Please email me if you can help.
Thanks in advance,
George
George Lawton Chief Electron Microscopist Molecular and Cellular Imaging Facility UT Southwestern Medical Center at Dallas 5323 Harry Hines Blvd. Dallas, Tx 75390-9039 Phone: 214-648-7291 Fax 214-648-6408 eMail: George.Lawton-at-UTSouthwestern.edu
GR Newman introduced LR-White for Immuno-EM 1982/1983. The first article published was in Histochem J (1983,15,543-555). OK so far. But there was a earlier report in the Journal of Microscopy in abstract form. I know issue and pages from another cite (J Microscopy, 1982, p. 127, RP5-6), but I don´t know the TITLE ....! Who can help me? Tanks in advance, With best regards,
Michael Reiner University of Cologne, Germany Dept. of Anatomy I _______________________________________________________________________________ Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE: http://web.de Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP: http://freemail.web.de
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I would like to request of preparation samples from poly(styrene)/poly(butyl acrylate) dispersion with 100 nm particles size for measurements by JEOL type of transmission electron microscope. We have got some problems with dilution of latexes and with time of staining of carbon, too. We would like to confirm a core/shell particles morphology of samples prepared by two step seeded emulsion polymerization. We have got some problems with magnification and contrast expansion of core/shell particles,too. Thank you very much for your help.
Petra Volfova, PhD. student of Department of plastics and rubber, Slovak Technical University,Bratislava,Slovak Republic.
NEWMAN GR, JASANI B, WILLIAMS ED THE PRESERVATION OF ULTRASTRUCTURE AND ANTIGENICITY J MICROSC-OXFORD 127: (SEP) RP5-RP6 1982
Chris
----- Original Message ----- } From: "Michael Reiner" {Elektronenmikroskopie-at-web.de} To: {Microscopy-at-sparc5.microscopy.com} Sent: Saturday, February 10, 2001 12:13 PM
Dear members of the listserver,
GR Newman introduced LR-White for Immuno-EM 1982/1983. The first article published was in Histochem J (1983,15,543-555). OK so far. But there was a earlier report in the Journal of Microscopy in abstract form. I know issue and pages from another cite (J Microscopy, 1982, p. 127, RP5-6), but I don´t know the TITLE ....! Who can help me? Tanks in advance, With best regards,
Michael Reiner University of Cologne, Germany Dept. of Anatomy I ______________________________________________________________________ _________ Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE: http://web.de Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP: http://freemail.web.de
I did some experiments with my SEM and external frame grabber. The results were rather interesting.
SEM is an Amray 1910FE, which includes internal TV signal processing, which is frame averaging and Kalman filtering.
External frame grabber is a Soft Imaging GrabBit PCI board controlled by their analySIS software. The analySIS and GrabBit package will do a huge number of frame averages. It will not do Kalman.
If I do frame averaging in the SEM at a count of 4 or 5, I get rather good results. If I do Kalman, I get very good results. Either of these frame processes can be frozen in the SEM's internal buffer. That composite NTSC RS-190 video is grabbed by the GrabBit. The captured results are those seen on the SEM's monitor.
If I do frame averaging with the GrabBit and analySIS anywhere from 8-30 frames, the results are not near as good as using the SEM's electronics. At low to medium magnification ( { 10KX), running at 80 averaged frames makes no dramatic difference. As magnification increases, the image tends to slightly shift. This effectively kills the ability to average. However, the SEM's internal Kalman filter will capture a very nice image in about 2-3 seconds. And this is quick enough to avoid image movement.
So, to answer your question, I would suggest looking for a TV frame grabbing system which does Kalman. No idea if such a thing exists. It would probably be an integrated h/w + s/w product since they would have to be closely tied. Since it is a real time process, the software would have to process image frames directly from the frame grabber board/interface.
If you need any other info, please contact me.
gary g.
At 02:41 AM 2/7/01, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Question: I wanted to know whether there are any books available which issues the topic of SEM analysis of optical fibers, which helps in analyzing the SEM images of optical fibers for Break source Analysis. Also I would like to know whether there are any other materials on this subject
} } } Hi, } } I am trying to identify an onset of apoptosis in endothelial cells (brain } } tissue) using postembedding immunostaining method. So far I have tried 11 } } different AB's and have not } } been able to stain the cells I know should be staining. Tissue has } } already been embedded in lowacryl so i have no choice in the run up. Any } } suggestions???? Neelima Shah.............. Biomedical Imaging Core Facility Uni of Pennsylvania Philadelphia, Pa.
Many years ago, probably during the mid '60's, I saw an instrument that was described to me as a "Beta Probe". It was used in the lab of a company selling non-ferrous metals (principally brasses and bronzes), and was an analytical instrument which determined the composition of the products with enough precision to qualify them for government specifications.
I remember parts of the demonstration I was given. A chunk of the material was ground and polished, then simply set on an O-ring seal on the top of the device. It was evacuated, the "Go" button was pushed, and after a suitable time (and perhaps other manipulation) some counters were read. Presumably these numbers were then converted, via a calibration, to the composition.
At the time I had no clue what it was actually doing. Now I surmise that it was an electron probe (probably with a "macro" spot size) with pre-set crystal spectrometers.
If anyone knows anything more about this device, both I, and my aunt (who managed the chemical part of the laboratory at that time - hence my visit!) would be fascinated to know. Many thanks.
I am member of few entomological listserver, even I am owner and moderator of coleoptera listserver. What I do not understand why only this listserver has spam messages. But When I try to write any message I am rejected..
Polarized Light Microscopy April 21, 28, May 5, 12, 2001
An advanced course on polarized light microscopy which will cover the following topics: The nature of polarized light The origin and interpretation of interference colors Birefringence and crystal orientation The Indicatrix Compensation and variable compensators Interference figures and their interpretation
The workshop will consist of four consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy. The course instructors include Jan Hinsch of Leica, Inc., Mary McCann of McCann Imaging, John Reffner of Sensir Technologies and N.Y.M.S. Instructor Don O'Leary.
WHEN: April 21, 28, May 5, 12, 2001 from 10 A.M. to 4 P.M.
WHERE: “Evergreens”, 30 N. Mountain Avenue, Montclair, NJ 07042 (accessible by public transportation, Information on car pools and transportation will be provided.)
COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership) Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use.
HOW: Register using the form below. Limited to the first 12 registrants. Return form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.
FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849
PLEASE POST ---------------------------------------------------------------------------- ------------------------------------- Registration Form Polarized Light Microscopy
One of Murphy's laws says that whenever something is published, it's immediately out of date. Project MICRO's "Microscopy for Children: a Bibliography", which was distributed with the December "Microscopy Today", is no exception. I've been contacted recently about two CD-ROMs that will interest many listserver readers. Since the web version of the bibliography (URL below) won't be updated for a while, I'm providing listings here:
Edmark Corp. 1998 Zap! $29.95, with a 50 page users' manual, from Edmark at PO Box 97201, Redmond, WA 98073-9721, 800-320-8379, www.edmark.com. For Macintosh or Windows 3.1 or 95. Subtitled "Save the show with sound, light, and electricity", this CD-ROM is sure to capture the interest of a computer game-addicted middle schooler. All three topics are introduced in game format, complete with levels of complexity and lots of shooting. In the optics section, laser beams are aimed at mirrors, lenses, prisms, and filters; the targets are eggs that hatch into cute monsters when activated by the beam. It actually does a very good job of teaching reflection, refraction, absorption, and color. The player who works through all levels of all three units then can set up the light show for a concert, and even manipulate the sound. It's much too time consuming to use in the classroom, but it's a delight for home use. There is ample reference material, and homeschool parents can set options to control complexity. Middle school. RECOMMENDED
Excalibur Mineral Company 1999 Photographic Guide to Mineral Species $69.95+ $4.00 shipping from Excalibur at 1000 North Division SStreet, Peekskill, NY 10566, www.bestweb.net/~excalmin. For Windows or Mac; requires Netscape Communicator (included). This is an "adult" CD, but the superb photos make it an excellent visual supplement for a student who is learning about crystals and other minerals. It's truly a "coffee table book" on a CD, with an unbelievable 5400 photos, which search and load rapidly in Netscape (and not at all in Internet Explorer). There is an eye-catching 300 image automatic slide show which can be set to play continuously. Images can be searched by location or mineral content. They are mostly micromounts, photographed at dissecting scope magnifications. Descriptions are minimal; it isn't intended as a textbook substitute. Adult. RECOMMENDED
Want more? There are over 20 on the website. Want copies of the bibliography for educational use? Contact me.
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
Dear Neelima, wow, 11 different antibodies tested is a lot! I don´t think that your problem is the choice of the antibody, you are enfaced with a more general problem.
Let´s assume your sections are about 60 nm thick. This is about a 100 times thinner than a conventional paraffin section and about 200 times thinner than a conventional cryosection. Thus, you have to consider that the amount of accesible epitopes/signal is 100-200 times weaker as seen in LM. Then, your samples are already embedded in resin. I don´t know what kind of procedure you applied, but consider a factor } = 10 of epitopes destroyed during embedding. That means at least 1000 times less epitopes in the same visible area as in LM-IHC. Impressive.
Now the other way round: Let´s say a section of 1mm in square of (mouse) brain cortex contains about 50 capillaries and 3-4 greater vessels, the capillaries containing 2-3 endothelial cells hit. That makes altogether, mh, 200 endothelial cells in your section (not whole cells, only a 60 nm slice of them...). I don´t know if you are examing normal tissue or pathological alterated (hypoxic, transgenic, inflamed, radiated etc...) but generally the apoptosis rate of adult continous endothelium is quite low. Let´s say rate of apoptosis is 2%, that would mean that 3-4 cells in your 1mm in square area are apoptotic! Common apoptosis markers refer to the nucleus. If 25% of all the endothelial cells show a nucleus hit by the section plane - expect one cell positive. Even if apoptosis rate is 10% there would be optimistically 5 cells, including those who are in the holes of your section after the immunoprocedure :-(
In my point of view, you are looking for the needle in the haystack.
If I had to look for apoptotic cells by immunohistochemistry, I would take thick sections of mildly fixed tissue on a vibratome and do a discrete DAB-reaction-based IHC after the use of a detergent (e.g. AB against p18 of PARP). Then embed the sections with positive cells after osmification and focus on trimming your blocks towards the cells positive. (a pity that you have no choice in the run-up)
In the proceeded apoptosis of the cell you can observe typical changes in the nucleus and cytoplasm, so the use of an antibody is not the only way to look for apoptosis at EM level.
I´m curious what the other people on the listserver have to say to this topic.
I wish you good luck and cross my fingers for you.
Best regards, Michael
Michael Reiner University of Cologne Dept. of Anatomy I Germany
Neelima Shah wrote: Hi, I am trying to identify an onset of apoptosis in endothelial cells (brain tissue) using postembedding immunostaining method. So far I have tried 11 different AB's and have not been able to stain the cells I know should be staining. Tissue has already been embedded in lowacryl so i have no choice in the run up. Any suggestions????
Neelima Shah Biomedical Imaging Core Facility Uni of Pennsylvania Philadelphia, Pa.
_______________________________________________________________________________ Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE: http://web.de Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP: http://freemail.web.de
Urgent request Is there anyone out there in cyber space that may know where we can obtain the metal band (LKB part number 90-00-0047) that hold the specimen arm up on an LKB Nova ultramicrotome. Any help would be gratefully appreciated
Terry
Dr Terry Robertson (PhD) Senior Research Fellow Department of Pathology University of Western Australia Nedlands 6009
Urgent request Is there anyone out there in cyber space that may know where we can obtain the metal band (LKB part number 90-00-0047) that hold the specimen arm up on an LKB Nova ultramicrotome. Any help would be gratefully appreciated
Terry
Dr Terry Robertson (PhD) Senior Research Fellow Department of Pathology University of Western Australia Nedlands 6009
I would like to purchase the Photoshop image software package. Unfortunately our computer department does not have any information on the cost of this package or where it can be purchased. Who can I contact for this information ?
Regards W. Erasmus
Willem Erasmus Snr. Scientist, Basic Catalysis Research Sasol Technology Tel : +27 +16 9603954 Fax : +27 +16 9602826 E-mail : willem.erasmus-at-sasol.com PO Box 1, Sasolburg, 1947, Republic of South Africa
[All views expressed are my own and not necessarily that of my employer.]
Leica now handle LKB/Reichert microtome parts if you can still get them. I am trying to trace a part in the UK for myself so I will contact you if they come back with any information on your metal band, it may take two weeks though. You could however try contacting Leica Australia see web address for details: Main web page http://www.leica-microsystems.com/ Australian website http://www.leica-microsystems.com/cgi-bin/boxgate29910?login=guest&password=&opt=area%3DCompany
I get the impression that the normal electron microscope rule of at least 10 (maybe 15 if you're lucky) years of availability for spare parts won't apply to ultramicrotomes because of the major changes in the market about 12 or 13 years ago (in the UK: LKB ---} Reichert ---} Cambridge Instruments? ---} Leica).
Good luck
Malcolm
Malcolm Haswell e.m. unit University of Sunderland UK
Terry Robertson wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Urgent request } Is there anyone out there in cyber space that may know where we can obtain the metal band (LKB part number 90-00-0047) that hold the specimen arm up on an LKB Nova ultramicrotome. Any help would be gratefully appreciated } } Terry } } Dr Terry Robertson (PhD) } Senior Research Fellow } Department of Pathology } University of Western Australia } Nedlands 6009 } } Phone 618 9346 2935 } Fax 618 9346 2891 } Mobile phone 040302 5440 } email terryr-at-cyllene.uwa.edu.au
2001 MICROSCOPY TECHNICIAN SUMMER POSITION AVAILABLE
The Marine Biological Laboratory has a summer position available for 10 to 15 weeks (June, July, and August) for a microscopy oriented technician. We would like to attract someone with some knowledge of biological preparative techniques and experience in any of the following: laser scanning confocal microscopy, TEM, SEM, and/or LM.
The technician will assist in the Central Microscopy Facility. The technician's duties will be to check out incoming investigators in the usage of our equipment and then to supervise its continuing usage and to perform contract work for investigators. This may include fixation, embedding, sectioning, scope use, darkroom work, etc. The technician will also provide routine maintenance.
This is a short term and scientifically rewarding position. Salary will be in the $8 to $10/hour range. Housing may be available to rent through MBL.
For more information, including a more detailed position description, please contact Louis Kerr at: MBL, 7 MBL Street, Woods Hole, MA 02543. Telephone, 508-289-7273; or at Email: lkerr-at-mbl.edu.
Please apply to: Human Resources, MBL, 7 MBL Street, Woods Hole, MA 02543. or resume-at-mbl.edu.
You can visit our web site for online employment information and general information regarding the Marine Biological Laboratory - http://www.mbl.edu/
An Equal Opportunity/Affirmative Action Employer/ Non-smoking workplace.
I'm looking for a service (in the USA) where I can send my TEM blocks to be sectioned and stained (UA/LC). These are biological samples cured in Embed 812 resin.
Lawrence Mason Research Scientist Genetics Institute lmason-at-genetics.com
Mayo Clinic has an opening for an EM technician in the Muscle Research Lab at the Rochester, MN campus. Details are as follows:
Research Technologist ñ Posting #01-0818
Summary:
Responsible for preparation of specimens for electron microscopy, evaluation of specimens in electron microscope, and related photography work. Will participate in research projects involving transmission EM, EM cytochemistry, and immunocytochemistry; data analysis including morphometry; assist in preparation of manuscripts for publication. Innovative and creative individual capable of independent work with minimal supervision is preferred. Executes experimental studies in support of lab goals, grant commitments. Assists in the planning, design and modifications of experiments. Performs basic statistical data analysis; prepares tables/charts/graphs and assists in organizing data. May make presentations and may contribute to writing of manuscripts. Trains residents/fellows/temporary lab personnel in lab techniques. Maintains lab equipment and supplies.
Qualifications:
Requires a Bachelor's degree in biology, chemistry, or other relevant sciences. Experience required in lab projects/research work in educational or work setting which would provide a solid understanding of lab techniques, equipment, and safety. Must have the ability to organize and carry out basic techniques independently. Candidate should have the ability to work both independently and in a team. Experience with electron microscopy is preferred.
For additional information on this position refer to posting # 01-0818 and contact:
Jill M. Kelly Staffing Specialist Mayo Clinic - Ozmun East -4 kelly.jill-at-mayo.edu ph #(507) 284-0510 fax# (507)284-9751 Assistant: Leah Carlson (507)284-3574
I am very interested in getting softwares to process electron diffraction patterns from disordered materials to obtain the G(r) (reduced intensity function), and J(r) (radial distribution function).
Any information on how to get these softwares is appreciated. In particular, information on free ones are most welcome.
Regards Yan Xin
======================================= Yan Xin (Ph.D) Magnet Science & Technology National High Magnetic Field Laboratory Florida State University 1800 E. Paul Dirac Drive Tallahassee, FL 32310 Tel: (850) 644 1529 Fax: (850) 644 0867 ========================================
Qualified candidates (Ph.D. or other terminal degree) are sought for a NIH-funded postdoctoral position to study intracellular calcium signals and their downstream effectors which regulate myofibril assembly. Primary training in real-time fluorescence digital microscopy (both conventional and confocal) using calcium tools and fluorescently tagged sarcomeric proteins expressed or microinjected in cultured myocytes. Prior training in protein biochemistry or molecular biology highly desirable. Interests and skill areas could include: cell signaling, cytoskeleton, actin or myosin dynamics, intracellular calcium channels, muscle development. Salary and start date are negotiable, funding available summer 2001. Please reply offline. See website below for more info.
Mike
Michael B. Ferrari Department of Biological Sciences University of Arkansas Fayetteville, Arkansas 72701 Ph: 501-575-6372 Fax: 501-575-5349 http://biology.uark.edu/ferrari
Has anyone out there in immuno cyber space had any experience in demonstrating complement C3 or C5 on formalin fixed frozen or paraffin sections. I need a good antibody that works. Any comments would be gratefully received.
Terry
Dr Terry Robertson (PhD) Senior Research Fellow Department of Pathology University of Western Australia Nedlands 6009
Someone from another lab that used to do EM, but doesn't do EM any more just delivered a couple of boxes of old resin components to me to 'take care of'.
It was OK with me, being the EM guy I should know what to do with them, but I realized I wasn't sure. I already had a bunch of old resins left over from kits folks had bought through the years and I figured I could just add it to the pile, but now there is a space problem, too may old bottles and not enough space to keep them.
These things are old, 1980's, but could they still be good enough to use? Is it worth finding space for them in case some poor starving student comes along who can't afford new supplies but is dying to do EM? What is the shelf life of things like this?
What would you do? Mix up a bunch of paper weights? Call EH&S and have them take away the bottles of individual components? Consolidate the nearly empty bottles to save space? Would you ever use things this old, I don't think I would. I am tempted to just get rid of them in one way or another. But I am haunted by the possibility that I will be tossing perfectly good stuff.
Relieve me of my guilt, tell me what to do.
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
Don't waste your time, disgard the lot. Regards JVN
Jon Krupp wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi: } } Someone from another lab that used to do EM, but doesn't do EM any more } just delivered a couple of boxes of old resin components to me to 'take } care of'. } } It was OK with me, being the EM guy I should know what to do with them, but } I realized I wasn't sure. I already had a bunch of old resins left over } from kits folks had bought through the years and I figured I could just add } it to the pile, but now there is a space problem, too may old bottles and } not enough space to keep them. } } These things are old, 1980's, but could they still be good enough to use? } Is it worth finding space for them in case some poor starving student comes } along who can't afford new supplies but is dying to do EM? What is the } shelf life of things like this? } } What would you do? Mix up a bunch of paper weights? Call EH&S and have them } take away the bottles of individual components? Consolidate the nearly } empty bottles to save space? Would you ever use things this old, I don't } think I would. I am tempted to just get rid of them in one way or another. } But I am haunted by the possibility that I will be tossing perfectly good } stuff. } } Relieve me of my guilt, tell me what to do. } } Jonathan Krupp } Microscopy & Imaging Lab } University of California } Santa Cruz, CA 95064 } (831) 459-2477 } jmkrupp-at-cats.ucsc.edu
-- John Nailon Operations Manager The Centre for Microscopy and Microanlaysis The University of Queensland St Lucia QLD 4072 Tel: +61-7-33654214 Fax: +61-7-33654422 WWW: http://www.uq.edu.au/nanoworld
Job Hunters, There was a job offer on the server last month from RangeTS-at-aol.com for an SEM tech job in Florida. Did anyone send any info and, if so, did anyone get a reply? Concerns have been expressed to me about having sent off personal info to a blind add and then getting no reply.
If RangeTS-at-aol.com is lurking out there, please have the courtesy to reply to all who answered your add ASAP. Taking personal info without acknowledgement or info on who you are is rather unprofessional. Blind adds are not the best way to go, but if you feel you must use them, the good folks on this listserver expect a professional response. Thank you.
Ken Converse owner Quality Images third party SEM service Delta, PA 17314
I am interested in characterizing emulsion latex Pressure Sensitive Adhesives (PSA) in their aqueous phase state and as cast films. Can anyone offer any specific techniques tips for doing cryo- ultramicrotomy of such material? Are there any good literature references that would be informative? Any recomendations for staining techniques of Acrylic based copolymer based adhesives?
At 04:38 PM 2/12/01 -0800, Jon Krupp wrote: } Someone from another lab that used to do EM, but doesn't do EM any more } just delivered a couple of boxes of old resin components to me to 'take } care of'.
I wholeheartedly encourage this kind of behavior... Tell the mailing list about old stuff you're about to throw away!
Someone out there might be willing to pay the cost of shipping and handling to get it, and perhaps they'll put it to good use.
It may not need to be fresh, contemporary or professional quality; don't forget the schools, teachers, amateurs, etc.
Hello All, Can anyone suggest/recommend a fixation protocol that preserves microtubules and/or centrioles without needlessly extracting other cellular components?
Thanks for any response, Jay Campbell University of Wisconsin Microscopy Resource
I sent some of the requested info, but I held some back just in case the request was bogus. It seems my fears are justified, as I never received a reply. On the other hand, I have not received any new spam, so perhaps it's not a diabolical plot. ;-)
I do hope, if it was a legitimate offer, that RangeTS-at-aol.com will take the time to respond to those who expressed an interest.
David Bell Scientist Electron Microscopy Lab Millipore Corporation 80 Ashby Road Bedford, MA 01730 (781) 533-2108
Ken Converse {qualityimages-at-n To: "MSA, listserver" {Microscopy-at-sparc5.microscopy.com} etrax.net} cc: Subject: Re:job offer from RangeTS-at-aol.com 02/13/01 06:47 AM
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Job Hunters, There was a job offer on the server last month from RangeTS-at-aol.com for an SEM tech job in Florida. Did anyone send any info and, if so, did anyone get a reply? Concerns have been expressed to me about having sent off personal info to a blind add and then getting no reply.
If RangeTS-at-aol.com is lurking out there, please have the courtesy to reply to all who answered your add ASAP. Taking personal info without acknowledgement or info on who you are is rather unprofessional. Blind adds are not the best way to go, but if you feel you must use them, the good folks on this listserver expect a professional response. Thank you.
Ken Converse owner Quality Images third party SEM service Delta, PA 17314
Warren wrote the following: ======================================================= I am interested in characterizing emulsion latex Pressure Sensitive Adhesives (PSA) in their aqueous phase state and as cast films. Can anyone offer any specific techniques tips for doing cryo- ultramicrotomy of such material? Are there any good literature references that would be informative? Any recomendations for staining techniques of Acrylic based copolymer based adhesives? ======================================================= This is not a question that is easily answered because you have to state further just what are your analytical objectives for wanting to do this work in the first place.
But if a typical (some are not "typical") acrylic emulsion, then the "particles" have to be "hardened" up in some way, otherwise they will coalesce and pancake, and when viewed by TEM will, otherwise, be one big ambiguous mess. So the soft particles have to be hardened up (into what look like hard rigid "marbles") and that is generally done using UV irradiation in quartz tubes or sometimes using RuO4. We get the best results on these kinds of emulsions with samples lightly shadowed with Pt/C.
For the sectioning of the resulting adhesive film, again this is a TEM job, you have to define whether you want to look at the interface with the release paper or some other characteristics, such as the dispersion of inorganic additives in the adhesive coating. Evidence of the release layer can sometimes be resolved this way as well. The preparation methods are different (for the different objectives) but the common feature is that they all must be cryo-sectioning using a good state of the art cryo- ultramicrotome and using only good quality diamond knives. Anyone who has either tried or done this knows that it can take one quite a long time to develop the magic of the "art" before good sections can be made on a reproducible basis.
I would be happy to give you more information off-line or better yet, our laboratory services division would be very happy to give you a quote to do the work for you.
Disclaimer: Our firm manufactures and distributes the kinds of materials and supplies needed for the conduct of this type of work and when one prefers to send it to an outside laboratory, we also perform studies on these kinds of samples for clients in our own facilities.
Chuck
PS: Please remember that we are nearly 100% paperless and we would ask that any reply to this message be by way of the "reply" feature on your software, so that the entire string of correspondence can come back to us and all be in one place.
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
} Hello All, } Can anyone suggest/recommend a fixation protocol that preserves } microtubules and/or centrioles without needlessly extracting other } cellular components? } } Thanks for any response, } Jay Campbell } University of Wisconsin } Microscopy Resource
You might have a look at "Increased visualization of microtubules by an improved fixation method". Luftig, R. B. et al. J. Histochem. Cytochem. 25:175-187, 1977. I seem to recall someone using PIPES as a buffer for glut fixation to improve microtubules.
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
Hi Jay, You didn't say what kind of cells so I won't comment specifically. But in general a good fix for tubes will have 10 mM EGTA to chelate calcium. A typical fix might contain 4 % paraformaldehyde, 0.1% glut, 25 mM Pipes pH 7.0, 10 mM EGTA, 1 mM MgSO4. Remember to fix at room temp, at least for the first half hour or so, because microtubules are cold sensitive. Hope this helps, Tobias
} } } } Hello All, } Can anyone suggest/recommend a fixation protocol that preserves } microtubules and/or centrioles without needlessly extracting other } cellular components? } } Thanks for any response, } Jay Campbell } University of Wisconsin } Microscopy Resource
I have extensive experience in all types of biological specimens and TEM. If you would like to discuss your project and estimated costs, please contact me at 716-275-1954. Our EM laboratory is housed in the Pathology Department, but serves as a core facility to any University of Rochester Med. Ctr. researchers. We also do projects for companies and researchers outside the University. So please call if you are interested in a discussion...
Sincerely,
Karen L. Jensen, M.S. Project Manager & Associate Scientist Electron Microscopy Research Core, Box 626 University of Rochester Medical Center Rochester, NY 14642
I disagree with JVN, because if the bottles have never been opened then they can definitely be used. Other EM technicians I have surveyed here at the University of Rochester Medical Center in Rochester, NY have used resins purchased in the 1980's in the 1990's-2000. So, if they are resins that you routinely order, start using the older bottles. Save yourself some money! Also you can test embedd a specimen. By the way if any of the resins are Lowicryls, they are definitely OK unless they have turned color, should be white not pale yellow. Also, here at the U of R we have a chemical recycle list that is accessible to other researchers here, thereby providing an opportunity to use unopened resins, chemicals, etc...Maybe your university has a similar program.
Karen L. Jensen, M.S. Project Manager & Associate Scientist Electron Microscopy Research Core
Petra Volfova wrote: } } I would like to request of preparation samples from } poly(styrene)/poly(butyl acrylate) dispersion with 100 nm particles size } for measurements by JEOL type of transmission electron microscope. } We have got some problems with dilution of latexes and with time of } staining of carbon, too. We would like to confirm a core/shell } particles morphology of samples prepared by two step seeded emulsion } polymerization. We have got some problems with magnification and contrast } expansion of core/shell particles,too. } Thank you very much for your help. } } Petra Volfova, PhD. student of Department of plastics and rubber, } Slovak Technical University,Bratislava,Slovak Republic. }
Dear Petra, Below are my experiences with TEM on polystyrene/poly(butyl acrylate) latexes. Please note that I'm not a specialist in this area and these are _not_ 100% perfect receipts - but it often works for me. Dilution: Wet the end (1...2cm) of a glass stick with your latex and dip it in a test tube filled with ca. 10 ml dist. water. The water must become slightly opaque. Repeat one or two times (depending from the concentration of the latex). Staining: If poly(butyl acrylate) forms the shell, some negativ staining could be helpful, because the acrylat phase itself is often not visible in TEM. I used uranyl acetate, two drops of 2% UA solution in 10 ml diluted latex. The PS-phase can be stained by vapour staining with Ruthenium (0.1g RuCl3 + 5 ml Na-Hypochlorid solution (13% Cl)). Preparation: I've mounted copper grids, coated with a 10 nm carbon film, on a specimen holder (from a TESLA BS500 TEM) and placed a drop diluted latex on the grid (formvar or collodium coated grids are not recommendable, because these films are very unstable under the electron beam after Ru-staining). After drying (some hours at room temp.) the grid can be umounted for vapour staining. If you have an "ultrasonic fogger" (or how this device is called in english, in german it's an "Ultraschallvernebler"), you can use it with very good results for preparation of latices smaller than ca. 200 nm. Microscopy: There is nothing special, if you have problems with the contrast, play a little bit with the accelerating voltage and objective aperture. Magnification should be not the problem. In polystyrene-core/poly(butyl acrylate)-shell particles the core/shell structure should be visible (if the acrylate shell is not too thin). If PS forms the shell, I've never seen a core/shell structure. In this case, you must embed the dryed latex particles in a resin (acrylate or epoxy), cut ultrathin sections and stain them for visualization of the core/shell structure (if the particles are not crosslinked, they may be soluble in some kind of resin, especially at higher temperatures).
Literature, that may help: A.W. Robards, A.J. Wilson (editors): Procedures in Electron Microscopy; John Wiley and Sons, 1993-1998 (a comprehensive collection of receipts, eg. staining methods, preparation of carbon and formvar coated grids etc.)
A technical position is available at the Eye Research Institute, Toronto Western Research Institute, University Health Network. Experience in immunohistochemistry and histology is essential, and a background in molecular biology would be beneficial. The position can either be a full time junior post, or part time senior post. The successful candidate will assist investigators who study the molecular mechanisms of eye development and disease. A CV and the names/phone numbers of two referees should be sent to Rod Bremner,
Judy Trogadis Vision Science Research Program Toronto Western Research Institute 399 Bathurst St. Toronto, ON M5T 2S8, Canada ph: 416-603-5088 fax: 416-603-5126 email:judy-at-uhnres.utoronto.ca
Dear Fellow Microscopists...I'm looking for suggestions on how to plan an undergraduate forensic microscopy course. Chances are the course will have to start with a generic introduction to microscopy, so anything on general undergraduate microscopy would also be appreciated. Books? Demonstrative Aids? Personal experiences with undergrad instruction, Etc. Any and all help would be appreciated. Thanks in advance for your time! Mary-Jacque Mann
One further comment after Michael's excellent summary.
If you can see any apoptotic cells in the sections, you shoud try and label these same cells using neighbouring sections. If these don't stain, you have a problem. You might also try and beg/borrow a Lowicryl embedded sample known to have reacted positively for an apoptotic marker, so that at least you can see if your antibody works. But really, apoptosis is such a rapid event that ultrastructural ID is perhaps as good as labelling, depending of course on why you want to label them. I seem to remember that apoptosis is complete in 20 mins. Shoot me down if I'm wrong!
Diana --
Diana van Driel Department of Clinical Ophthalmology Sydney University GPO Box 4337 Sydney NSW AUSTRALIA 2001
Jay, Sorry, I forgot one thing! Tobias Baskin´s proposal for a MT fix sounds good as well. EGTA seems to me useful. But anyway, what kind of fixative you will choose, DON`T forget to keep your fixative at room temperature at least, this is a crucial prerequisite.
Michael ______________________________________________________________________________ Die Fachpresse ist sich einig: WEB.DE 18mal Testsieger! Kostenlos E-Mail, Fax, SMS, Verschlüsselung, POP3, WAP....testen Sie uns! http://freemail.web.de
Hello Jay! Try it with a fixative containing glut and tannic acid (TA), followed by osmification and embedding in epoxy. There is a classical paper:
Lewis G. Tilney et al.: "Microtubules: Evidence for 13 Protofilaments", JCB 59,pp 267-275, 1973
Tilney used 2% Glut and 2-8% tannic acid made in phosphate buffer. 1% digitonin was added to faciitate TA penetration.
My recipe is the following: make a sticky TA solution: solve 25 grs TA powder in 25 ml aqua, heat on a stirrer until you have a dark brown solution, be quick, so it will remain fluid. Filter with a paper filter . The solution made is stable over years, the older it gets the better your result. For fixation add 1-2% v/v of the stock TA solution to a cacodylate/phosphate buffer containing 4% Formaldehyd and 2% Glutaraldehyde. May be you will have to add 0,5-1% digitonin, but TA from the stock solution penetrates not that bad as directly in the fix soluted TA powder will do. Don´t fix your samples too long, because overcontrasting can occur (max. 24 hours). Then, follow a standard embedding protocol including osmificartion and uranylic acetate en-bloc staining. Try a quick dehydration with aceton to avoid tissue component extraction but I have satisfactorily results with normal alchoholic dehydration.
Hope this helps!
Good luck, with best regards, Michael
Michael Reiner University of Cologne, Germany Dept. of Anatomy I
Jay wrote } Hello All, } Can anyone suggest/recommend a fixation protocol that preserves } microtubules and/or centrioles without needlessly extracting other } cellular components?
______________________________________________________________________________ Die Fachpresse ist sich einig: WEB.DE 18mal Testsieger! Kostenlos E-Mail, Fax, SMS, Verschlüsselung, POP3, WAP....testen Sie uns! http://freemail.web.de
To the person looking for ultrastructural evidence of apoptosis: Take a look at one of my papers: American Journal of Pathology, Vol. 156, No. 6, June 2000. Good Luck, Tim
Timothy G. Schneider Director of Electron Microscopy Department of Pathology Room 229 Jefferson Hall Thomas Jefferson University 1020 Locust St. Philadelphia Pa. 19107 215-503-4798 work 610-613-8170 cellular timothy.schneider-at-mail.tju.edu
here we are, trying to find out how to face a SEM work related to a request from someone who manufactures some kind of synthetic boards to build up ceilings. We do not know much about this product but, they ensure it does not contain asbestos. Actually, they need to proof that!.
How would you make up the measurements in order to garantee or proof that no asbestos at all are found in these boards. It means a kind of "negative experiment" what we are asked to do.
Any experiences or sugestions?. We are looking at standards or regulations but not found about the sampling and measuring (observation) procedure for a negative result.
Thanks in advance,
Silvia Montoro Centro Regional de Investigación y Desarrollo de Santa Fe Güemes 3450 3000 Santa Fe Fax +54 - 342 - 455 0944 e-mail: csedax-at-arcride.edu.ar smontoro-at-arcride.edu.ar npratta-at-arcride.edu.ar
I was recently asked who manufactures cryo stage/prep equipment for SEM's. We have had such equipment for several years, but I have no idea what other suppliers are out there. Any help? Bill
-- ============================================================= Bill Chissoe III Electron Microscopist University of Oklahoma 770 Van Vleet Oval Norman, Ok. 73019 E-mail: wchiss-at-ou.edu Ph. (405)325-4391 =============================================================
I want to say thanks to all people who replied to my inquiry on who can do EDS. I received 17 responses and/or suggestions. I was overwhelmed with the response. I have passed the information on to the investigator who will decide what direction she wants to go in.
I can't tell you how many times the listserver has come to the recuse. Sometimes a response comes while I'm thinking about asking the listserver. It like you are reading my mind. Besides being a lifesaver, it is also educational. I learn alot from all of you. Thanks again.
George
George Lawton Chief Electron Microscopist Molecular and Cellular Imaging Facility UT Southwestern Medical Center at Dallas 5323 Harry Hines Blvd. Dallas, Tx 75390-9039 Phone: 214-648-7291 Fax 214-648-6408 eMail: George.Lawton-at-UTSouthwestern.edu
I posted a message to say that we have a Gatan Dual Ion Mill to dispose of. We have been swamped with replies. I had no idea what I was letting myself in for. Moreover, I got flu and so have been unable, til now, to respond. So, please no new contacts. Also those who have made contact already, please be patient. I will get to deciding what to do as soon as I can.
-- .......... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
Emitech makes several models. You can read about them in our on online: Home} Contents} K4 Disclaimer: ProSciTech supplies these instruments, but only within Australasia south of Singapore. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Thursday, February 15, 2001 5:32 AM, Bill Chissoe [SMTP:wchiss-at-ou.edu] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I was recently asked who manufactures cryo stage/prep equipment for } SEM's. We have had such equipment for several years, but I have no idea } what other suppliers are out there. Any help? } Bill } } -- } ============================================================= } Bill Chissoe III } Electron Microscopist } University of Oklahoma } 770 Van Vleet Oval } Norman, Ok. 73019 } E-mail: wchiss-at-ou.edu Ph. (405)325-4391 } ============================================================= } }
Does anyone out there have experience in cleaning up fecal material so it can be processed for TEM. We are looking for a virus which we are going to label. Any help, anyone could give me ASAP would be greatly appreciated
Marie Jenkins Lab Manager Department of Environmental & Toxicologic Pathology Armed Forces Institute of Pathology Bldg 54 Rm M093B Washington, D.C. 20306-6000 Tel:202-782-2734 Fax: 202-782-9215 jenkins-at-afip.osd.mil
In a rationalisation of our institutes library we have come across two old volumes of the Illustrated Annual of Microscopy dated 1898 and 1900 published by Percy Lund, Humphries & Co London. Has anyone any information on the scarcity of these volumes so we can decide whether to donate them to a library with better archival storage facilities.
Thanks
Ian
Ian Hallett HortResearch Mt Albert Research Centre Private Bag 92 169 Auckland, New Zealand Fax 64-9-815 4201 Telephone 64-9-815 4200 EMail ihallett-at-hortresearch.co.nz
Gatan (incorporating the cryo EM bit of Oxford Instruments), VG Microtech (Polaron), EmiTech and Bal-Tech are main players. Their contact details can be found in the Microscopy Vendors Database
http://www.kaker.com/mvd/list.html Chris
Date sent: Wed, 14 Feb 2001 11:31:41 -0800 } From: Bill Chissoe {wchiss-at-ou.edu}
We often get dust and bits of board from the site here to examine. We use powder X-ray diffraction to test for asbestos. If you have a theta-2theta diffractometer you could use, I can give you a protocol.
Richard
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi dear microscopists, } } here we are, trying to find out how to face a SEM } work related to a request from someone who manufactures some } kind of synthetic boards to build up ceilings. We do not know much } about this product but, they ensure it does not contain asbestos. } Actually, they need to proof that!. } } How would you make up the measurements in order to guarantee or } proof that no asbestos at all are found in these boards. It means a } kind of "negative experiment" what we are asked to do. } } Any experiences or suggestions?. We are looking at standards or } regulations but not found about the sampling and measuring } (observation) procedure for a negative result. } } Thanks in advance, } } Silvia Montoro } Centro Regional de Investigación y Desarrollo de Santa Fe } Güemes 3450 } 3000 Santa Fe } Fax +54 - 342 - 455 0944 } e-mail: csedax-at-arcride.edu.ar } smontoro-at-arcride.edu.ar } npratta-at-arcride.edu.ar
e-mail richard.beanland-at-marconi.com Tel. +44 1327 356363 Fax. +44 1327 356398 ============================================================== "The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone. Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."
Dear Everybody, Our central science lab is trying to egt a coater unit, can any body suggest where to get one and what are the performance xteristics that goes with it also all neccesary accessories. thank You
Mr. O. O. ILORI DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING OBAFEMI AWOLOWO UNIVERSITY, ILE-IFE, OSUN STATE NIGERIA.
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Bill, Gatan has recently taken over the SEM cryostage previously marketed by Oxford Instruments (originally owned by Hexland in the 80's). My understanding is that the deal was the division in it's entirety which means that the personnel involved with tech support, sales, etc. also moved on to Gatan. Debby
Debby Sherman Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-099e Whistler Building West Lafayette, IN 47907
On Wednesday, February 14, 2001 2:31 PM, Bill Chissoe {wchiss-at-ou.edu} wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id IAA27120 for dist-Microscopy; Thu, 15 Feb 2001 08:53:33 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id IAA27117 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Thu, 15 Feb 2001 08:53:03 -0600 (CST) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id IAA27110 for {Microscopy-at-sparc5.microscopy.com} ; Thu, 15 Feb 2001 08:52:51 -0600 (CST) Received: from NotesHubEP.phi.com (noteshubep [10.10.0.10]) by dns.phi.com (8.8.8+Sun/8.8.8) with ESMTP id IAA27772; Thu, 15 Feb 2001 08:51:18 -0600 (CST)
To analyze for asbestos at least two approaches can be used. A small piece of the sample can be pulverized and characterized by PLM. A more complete method that will provide some quantitative measure is to pulverize a measured mass of the material and then put into a DI solution and sonicate. Aliquot of the solution are then redeposited onto special filters via vacuum filtration and then prepped for analysis by TEM/EDS. There are many labs in the USA that specialize in these types of analysis. You can contact them to get more detailed information on these types of analysis.
Hope this helps.
John Humenansky/Staff Scientist Physical Electronics, Inc. (PHI) 6509 Flying Cloud Drive Eden Prairie, MN 55344 952-828-6387
In America, there are lots of commercial asbestos testing labs. The analysis is usually performed by utilizing a TEM and/or a PLM based on protocols by NVLAP or other agencies. In your case, the most reliable +/- confirmation can be done with a TEM in 10 minutes, including sample preparation, should you have the permission to do asbestos analysis and be familiar with the morphology/structure(SAED)/chemistry(EDS). But I would like to suggest you send the sample out for the analysis because of the HAZMAT issue. It costs $60-$200 for one sample. For further information, you can contact me off line.
Disclaimer: I'm not doing asbestos analysis any more though had done it for a living for a couple of years, and nor do I have any financial interests in this kind of analysis.
-cy, Scientist, Rodel Inc.
-----Original Message----- } From: "csedax-at-alpha.arcride.edu.ar"-at-sparc5.microscopy.com [mailto:"csedax-at-alpha.arcride.edu.ar"-at-sparc5.microscopy.com] Sent: Wednesday, February 14, 2001 12:38 PM To: Microscopy-at-sparc5.microscopy.com Cc: csedax-at-ARCRIDE.EDU.AR
Hi dear microscopists,
here we are, trying to find out how to face a SEM work related to a request from someone who manufactures some kind of synthetic boards to build up ceilings. We do not know much about this product but, they ensure it does not contain asbestos. Actually, they need to proof that!.
How would you make up the measurements in order to garantee or proof that no asbestos at all are found in these boards. It means a kind of "negative experiment" what we are asked to do.
Any experiences or sugestions?. We are looking at standards or regulations but not found about the sampling and measuring (observation) procedure for a negative result.
Thanks in advance,
Silvia Montoro Centro Regional de Investigación y Desarrollo de Santa Fe Güemes 3450 3000 Santa Fe Fax +54 - 342 - 455 0944 e-mail: csedax-at-arcride.edu.ar smontoro-at-arcride.edu.ar npratta-at-arcride.edu.ar
Thanks to all who answered my inquiry about cryoSEM. As always, this is a very friendly, helpful group. Bill
-- ============================================================= Bill Chissoe III Electron Microscopist University of Oklahoma 770 Van Vleet Oval Norman, Ok. 73019 E-mail: wchiss-at-ou.edu Ph. (405)325-4391 =============================================================
It is my understanding that all asbestos testing with an Electron Microscopy Lab must acquire certification from not only their state health agency but also there are Environmental Protection Agency regulations, one must thoroughly examine. I would be very cautious to accept any work involving Asbestos testing. Several laboratories have been sued. We were also approached by a local company in Rochester, NY who would provide us with a substantial amount of money to test asbestos, but upon talking to the NY state Health Dept., and looking into the Technician's certification course(which is not cheap and is 1year), and the laboratory would also have to be licensed, we decided it wasn' t going to be worth it.
Karen L. Jensen, M.S. Project Manager & Associate Scientist Electron Microscopy Research Core
STOOL Samples of about 2-4 gm can be collected into a sterile container filled only half way. Make a 10-20% suspension in distilled water and clarify by centrifuging at 3000 rpm for 30 minutes. Then take the supernatant and centrifuge again at 15,000 RPM for one hour to concentrate a viral laden pellet. This pellet you could resuspend in various dilutions and drop onto a coated grid and immunolabel.
Karen L. Jensen, M.S. Project Manager & Associate Scientist Electron Microscopy Research Core
-----Original Message----- } From: "JENKINS-at-afip.osd.mil"-at-sparc5.microscopy.com [mailto:"JENKINS-at-afip.osd.mil"-at-sparc5.microscopy.com] Sent: Wednesday, February 14, 2001 8:34 PM To: microscopy-at-sparc5.microscopy.com
Does anyone out there have experience in cleaning up fecal material so it can be processed for TEM. We are looking for a virus which we are going to label. Any help, anyone could give me ASAP would be greatly appreciated
Marie Jenkins Lab Manager Department of Environmental & Toxicologic Pathology Armed Forces Institute of Pathology Bldg 54 Rm M093B Washington, D.C. 20306-6000 Tel:202-782-2734 Fax: 202-782-9215 jenkins-at-afip.osd.mil
The official deadline for submission of papers for the 2001 Microscopy and Microanalysis meeting is today, February 15. However, there have been some problems with the database so the deadline is being extended until Noon Eastern Time on Monday, February 19. All Title, Author and AV requirement data must be entered into the database at mmconference.org/2001 by that time and papers must be received at the following address by Tuesday, February 20.
MSA Meeting Managers 7000 West Southwest Highway Chicago Ridge, IL 60415
877-672-6271 (Telephone)
If you have problems submitting your Author information, or if you would like to submit a paper for the late breaking poster session after February 19, please contact Bob Price (Price-at-med.sc.edu) or 803-733-3393
Thanks.
Bob Bob Price M&M 2001 Program Chair 803-733-3393 (T) 803-733-1533 (F)
Manager Keck Center for Characterization of Nanoscale Soft Materials-Northwestern University seeks a Ph.D. scientist with experience in operation of imaging TOF-SIMS, high vacuum STM/AFM and high throughput XPS instruments to manage open user facility. For detailed job description see http://www.chem.nwu.edu/group_openings.htm. Applicants should send CV and summary of relevant experience and qualifications to Dr. Robert N. Scott, Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113 or to r-scott-at-chem.nwu.edu. EEO/AA Employer.
There is a paper in Applied Surface Science 167 (2000), pp. 59-68 entitled, "Structural studies of amorphous and crystallized tungsten nitride thin films by EFED, XRD, and TEM" by Y.G. Shen and Y.W. Mai that you should look up if you are interested in this topic.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
-----Original Message----- From: Yan Xin [mailto:xin-at-magnet.fsu.edu] Sent: Monday, February 12, 2001 12:32 PM To: microscopy-at-sparc5.microscopy.com Subject: software for RDF of amorphous materials
--------------------------------------------------------------- --------- The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html
I am very interested in getting softwares to process electron diffraction patterns from disordered materials to obtain the G(r) (reduced intensity function), and J(r) (radial distribution function).
Any information on how to get these softwares is appreciated. In particular, information on free ones are most welcome.
Regards Yan Xin
======================================= Yan Xin (Ph.D) Magnet Science & Technology National High Magnetic Field Laboratory Florida State University 1800 E. Paul Dirac Drive Tallahassee, FL 32310 Tel: (850) 644 1529 Fax: (850) 644 0867 ========================================
We are in the process to get a confocal microscope for a multi-user center. The major application for this microscope will be using green fluorescent proteins and /or styrul dyes (e.g., FM 1-43) to label specific neuronal pathways, including living cells.
Our consideration will be either "Leica TCS SP2" or "Zeiss LSM 510", any suggestion and advice on the comparison of these two microscopes will be highly appreciated.
Many thanks.
******************************************* Xinran Liu, M.D., Ph.D. Center for Basic Neuroscience UT Southwestern Medical Center 6000 Harry Hines Blvd., NA4. 214A Dallas, TX 75390-9111
WE have the Zeiss LSM 510 and it's great. Their customer support/technical support is outstanding.
Good Luck- Michelle Taurino Aventis Pharmaceuticals Senior Scientist Bioimaging and Molecular Histology Tel-908-231-3357 Fax-908-231-3962 e-mail: Michelle.Taurino-at-aventis.com
-----Original Message----- } From: Xinran Liu [mailto:xinran.liu-at-UTSouthwestern.edu] Sent: Thursday, February 15, 2001 7:50 PM To: Microscopy-at-sparc5.microscopy.com
We are in the process to get a confocal microscope for a multi-user center. The major application for this microscope will be using green fluorescent proteins and /or styrul dyes (e.g., FM 1-43) to label specific neuronal pathways, including living cells.
Our consideration will be either "Leica TCS SP2" or "Zeiss LSM 510", any suggestion and advice on the comparison of these two microscopes will be highly appreciated.
Many thanks.
******************************************* Xinran Liu, M.D., Ph.D. Center for Basic Neuroscience UT Southwestern Medical Center 6000 Harry Hines Blvd., NA4. 214A Dallas, TX 75390-9111
At 6:50 PM -0600 2/15/01, Xinran Liu wrote: } } } We are in the process to get a confocal microscope for a multi-user center. } The major application for this microscope will be using green fluorescent } proteins and /or styrul dyes (e.g., FM 1-43) to label specific neuronal } pathways, including living cells. } } Our consideration will be either "Leica TCS SP2" or "Zeiss LSM 510", any } suggestion and advice on the comparison of these two microscopes will be } highly appreciated. } } Many thanks. } **************************************
I cannot say anything about the Leica instrument, since I have never used it. I can however, speak about the Zeiss LSM 510. I operate a multi-user core facility and we have had an LSM 510 here since Nov. 1998. It has proven itself to be a reliable and fairly robust instrument. We have aver 50 registered users. Some work quite frequently, others rarely. Our regular users have all been trained to use the system and work independently. The types of samples examined also vary widely, from immunolabelled tissue sections (paraffin and cryo) to live cell culutres expressing GFP. Overall, we are very happy with it.
Good luck, Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
I have had several requests for the asbestos protocol, so here it is.
1) Immerse sample in 10% HCL for 30 mins or until bubbles have stopped. (This removes filler material, usually calcite ICPMS 5-586.)
2) Decant and wash in water.
3) Evaporate till dry.
4) Grind to a powder using pestle and mortar. (Obviously, if the sample is suspected to be asbestos, take suitable precautions!)
5) Mount as a standard thick powder sample for theta-2theta diffractometry
6) Obtain data from 5-60 degrees (theta) using Cu K alpha radiation. (We have a fairly standard Philips diffractometer, with graphite monochromator and acceptor slit.)
In the UK, the notifiable asbestos compounds according to act of Parliament (don't ask me which one!) are:
Other compounds which may be considered to be hazardous/asbestos-like are: Quartz (SiO2) ICPMS 33-1161 Tridymite (SiO2) ICPMS 18-1170 or 14-260 Christobalite (SiO2) ICPMS 11-695 Kaolinite ICPMS 14-164 Amosite ICPMS 29-1170 Anorthite ICPMS 18-1202 Phlogopite ICPMS 10-492
You will need the ICPMS database and the ability to compare and/or search for matches.
Detection limit is about 5%, depending on the other materials in the sample.
This is not an accredited test. If you have a sample which doesn't show up positive, it may still contain asbestos - if in doubt, get an accredited test house to check.
No liability is accepted or implied in providing this information.
e-mail richard.beanland-at-marconi.com Tel. +44 1327 356363 Fax. +44 1327 356398 ============================================================== "The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone. Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."
I have been looking for a copy of "Food Microscopy", by Olga Flint. It is one volume of the Royal Microscopy Society handbook series. I saw copies available during the last MSA meeting, but cannot recall who the vendor was. Can somebody point me towards a company or individual who may have a copy of this book available for sale?
Karl Hagglund, Researcher P&G Food and Beverage Analytical and Microbiology 6071 Center Hill Ave., Box 117 Cincinnati, OH 45224 (513)634-0146
I have been looking for a copy of "Food Microscopy", by Olga Flint. It is one volume of the Royal Microscopy Society handbook series. I saw copies available during the last MSA meeting, but cannot recall who the vendor was. Can somebody point me towards a company or individual who may have a copy of this book available for sale?
Karl Hagglund, Researcher P&G Food and Beverage Analytical and Microbiology 6071 Center Hill Ave., Box 117 Cincinnati, OH 45224 (513)634-0146
The US Environmental Protection Agency (US EPA) developed a series of methods [Polarized Light Microscopy, Transmission Electron Microscopy, Gravimetry (including acid dissolution and ashing), and X-ray Powder Diffraction] for the analysis of asbestos in building materials that are described in the following document:
EPA 600/R-93-116 Method for the Determination of Asbestos in Bulk Building Materials. July 1993. (NTIS / PB93-218576) [Updates and replaces Interim version in 40 CFR 763 Subpart F App A]
Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov Microanalysis Research Group Office: 301-975-3902 N.I.S.T. FAX: 301-417-1321 100 Bureau Drive, Stop 8371 Gaithersburg, MD 20899-8371 http://www.nist.gov/cstl/div837/837.02/
We also have a multi-user facility and have two Leica systems, one an inverted with multi-photon lasers and the other an upright with argon, krypton, and HeNe lasers. When they work, they're great. However, software has been less than desirable , as has service. Software keeps being "upgraded" but not necessarily improved. I'm sure all the instruments have problems but I think we've had more than our share with Leica.
At 06:50 PM 2/15/01 -0600, Xinran Liu wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Mary Gail Engle Sr. Research Laboratory Manager Electron Microscopy & Imaging Facility Health Sciences Research Bldg. 001 University of Kentucky Lexington, KY 40536-0305
I have (not) identified asbestos... That is, some insulating material was brought to me with the question, "Is it asbestos?". I was able to say that it was not since careful EDS examination of the powdered material revealed no trace of the elements associated with asbestos. It was 100% gypsum.
Even if not certified, this would seem to produce a satisfactory answer. ..Or are the regulations now so absurd that officialdom is concerned someone may mistake brass for asbestos? ;)
Woody White McDermott Technology, Inc. http://www.mtiresearch.com/
Need assistance on Gatan PIPS. Believe DP and MDP are functioning properly, but specimen chamber vacuum is "glitchy."
Symptoms: DP light on constant, MDP light never comes on, DP test reads 19.9 at all times, Lights on vac and vent do not glow nor will sample raise or lower.
Please respond off list.
J. Cindy Kleier Specimen Preparation Engineer Northwestern University Evanston, IL. USA 847-491-7807 j-kleier-at-northwestern.edu
fatbrain.com and barnes and noble and amazon.com all list this book for 20 something bucks. hope this helps...
ed sharpe archivist for SMECC
{ { Subj: help me to find a book Date: 2/16/01 2:06:41 PM US Mountain Standard Time From: hagglund.kw-at-pg.com-at-sparc5.microscopy.com To: microscopy-at-microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
I have been looking for a copy of "Food Microscopy", by Olga Flint. It is one volume of the Royal Microscopy Society handbook series. I saw copies available during the last MSA meeting, but cannot recall who the vendor was. Can somebody point me towards a company or individual who may have a copy of this book available for sale?
Karl Hagglund, Researcher P&G Food and Beverage Analytical and Microbiology 6071 Center Hill Ave., Box 117 Cincinnati, OH 45224 (513)634-0146
----------------------- Headers -------------------------------- Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: from rly-xb03.mx.aol.com (rly-xb03.mail.aol.com [172.20.105.104]) by air-xb05.mail.aol.com (v77_r1.21) with ESMTP; Fri, 16 Feb 2001 16:06:41 -0500 Received: from ultra5.microscopy.com ([206.69.208.10]) by rly-xb03.mx.aol.com (v77_r1.21) with ESMTP; Fri, 16 Feb 2001 16:06:23 -0500 Received: (from daemon-at-localhost) by ultra5.microscopy.c } }
We are e-mailing to let you know that our textbook:
"Transmission Electron Microscopy and Diffractometry of Materials"
has just been published by Springer. This textbook and its earlier drafts have been used for over a decade in courses on transmission electron microscopy (TEM) and x-ray diffractometry (XRD) at the California Institute of Technology and University of Virginia. These courses are taken by graduate students and advanced undergraduates in materials science, solid-state physics, and solid-state chemistry.
The book emphasizes themes common to both diffraction and microscopy, such as wave coherence, scattering from atoms, and the formation and analysis of diffraction patterns. It also describes the uniqueness of TEM and XRD. The book has 748 pages through the index, includes approximately 500 accompanying illustrations, and a total of 151 problems at the ends of chapters. The Appendix provides a set of introductory TEM laboratory exercises, and contains up-to-date reference data for both TEM and XRD. The chapters are:
1. Diffraction and the X-Ray Powder Diffractometer, p. 1 2. The TEM and its Optics, p. 63 3. Scattering, p. 123 4. Inelastic Electron Scattering and Spectroscopy, p. 167 5. Diffraction from Crystals, p. 225 6. Electron Diffraction and Crystallography, p. 275 7. Diffraction Contrast in TEM Images, p. 339 8. Diffraction Lineshapes, p. 423 9. Patterson Functions and Diffuse Scattering, p. 467 10. High-Resolution TEM Imaging, p. 523 11. Dynamical Theory, p. 595 12. Bibliography, p. 661 13. Appendix, p. 675 14. Index, p. 735
To help with teaching, sections containing specialized or higher-level material are marked, and paths around these sections are suggested. A secure web site has been established with worked solutions to 3/4 of the problems in the text.
A web site with excerpts from many chapters is:
http://www.caltech.edu/~matsci/btf/TEM_Book.html
Publication and order information can be found at the Springer site:
We hope that you find our book useful and look forward to hearing from you about it.
Sincerely yours, Brent Fultz and Jim Howe
Brent Fultz, Professor of Materials Science California Institute of Technology, mail 138-78 Pasadena, CA 91125, USA Office: 626-395-2170; Fax: 626-795-6132; E-mail: btf-at-hyperfine.caltech.edu
James M. Howe, Professor and Director of the Electron Microscope Facility Department of Materials Science & Engineering University of Virginia Charlottesville, VA 22904-4745, USA Office: 804-982-5646; Fax: 804-982-5660; E-mail: jh9s-at-virginia.edu
Hello, We are looking for some Wild 20x oculars to equip a Wild M5 stereo microscope. If anyone has any extras or knows of a source, please respond directly to: LMM7001-at-humboldt.edu
Thanks, Lewis mcCrigler Equipment Tech. Humboldt State University
I think this is fundamentally an EDS detection limit issue. Question is when you see Ca, S, and possibly O peaks only on a spectrum from your insulation, how sure you can be to say that you don't have 1 vol% of Si-Mg-Fe-Mn etc while the 1 vol% is the cut-off concentration in the US regulation governing asbestos analysis of building materials. Personally, I wouldn't be very comfortable with an EDS un-identification of asbestos.
-cy, Ex-expert of asbestos analysis
-----Original Message----- } From: White, Woody N. [mailto:nwwhite-at-mcdermott.com] Sent: Friday, February 16, 2001 4:02 PM To: 'Microscopy-at-MSA.Microscopy.Com'
Any comments on this one?
I have (not) identified asbestos... That is, some insulating material was brought to me with the question, "Is it asbestos?". I was able to say that it was not since careful EDS examination of the powdered material revealed no trace of the elements associated with asbestos. It was 100% gypsum.
Even if not certified, this would seem to produce a satisfactory answer. .Or are the regulations now so absurd that officialdom is concerned someone may mistake brass for asbestos? ;)
Woody White McDermott Technology, Inc. http://www.mtiresearch.com/
Forgot to mention that my previous comments only apply to EDS/SEM that appears to be what Mr. Woody meant. In the case of TEM analysis, Woody should be 100% right if the beam is focused on each individual fibers and sufficient number of fibers are checked.
-cy, Ex-fiber counter on TEM, PLM and PCM
-----Original Message----- } From: Ni, Chao-Ying Sent: Saturday, February 17, 2001 5:48 PM To: 'White, Woody N.'; 'Microscopy-at-MSA.Microscopy.Com'
Any comments on this one?
I have (not) identified asbestos... That is, some insulating material was brought to me with the question, "Is it asbestos?". I was able to say that it was not since careful EDS examination of the powdered material revealed no trace of the elements associated with asbestos. It was 100% gypsum.
Even if not certified, this would seem to produce a satisfactory answer. .Or are the regulations now so absurd that officialdom is concerned someone may mistake brass for asbestos? ;)
Woody White McDermott Technology, Inc. http://www.mtiresearch.com/
We recently had a Leica TCS SP2 installed, and it has been running just fine, software is easy to use and service has been great. In a previous institution we had two Leica TCS's - hardware fine, software OK, service also great.
IMHO, the main considerations are first, service and reliability - and service varies quite a bit with location for each company. Second, do you really need the SP function of the Leica? If not, I think these two instruments are much of a muchness for routine work. There are pluses and minuses on each side and you need to decide what your priorities are. Third, what kind of price/package can you negotiate?
Another 2¢ worth..... cheers,
At 06:50 PM 2/15/01 -0600, Xinran Liu wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Rosemary White Microscopy Centre CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
Hello all, I need to do some immunogold TEM with antibodies that work well for light microscopy immunohistochemistry but require various antigen retrieval treatments. I used to do a lot of immunogold in the old pre-antigen retrieval times. I wonder what are the current developments in this area. Are antigen retrieval techniques used on LR White sections? Thanks for any suggestions,
Sarka Lhotak, PhD.
Hamilton Regional Cancer Centre McMaster University Hamilton, Ontario, Canada lhotaks-at-mcmaster.ca
I understand the situation. In addition to "bulk" analysis, numerous locations were examined using higher and higher BSE magnifications to sort out any (non existent in this case) Z differences. EDS was used for each as well. In addition, with the appropriate choice of accelerating potential and count integral, my EDS could easily detect { 0.25%. Granted, this is not a very efficient method and if ANY of the questionable elements were detected, nothing is proved.
Woody
} Dear Woody, } } I think this is fundamentally an EDS detection limit issue. } Question is when } you see Ca, S, and possibly O peaks only on a spectrum from } your insulation, } how sure you can be to say that you don't have 1 vol% of } Si-Mg-Fe-Mn etc } while the 1 vol% is the cut-off concentration in the US } regulation governing } asbestos analysis of building materials. Personally, I } wouldn't be very } comfortable with an EDS un-identification of asbestos. } } -cy, Ex-expert of asbestos analysis } } } } -----Original Message----- } From: White, Woody N. [mailto:nwwhite-at-mcdermott.com] } Sent: Friday, February 16, 2001 4:02 PM } To: 'Microscopy-at-MSA.Microscopy.Com' } Subject: RE: Asbestos protocol } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } } Any comments on this one? } } I have (not) identified asbestos... That is, some insulating } material was } brought to me with the question, "Is it asbestos?". I was } able to say that } it was not since careful EDS examination of the powdered } material revealed } no trace of the elements associated with asbestos. It was } 100% gypsum. } } Even if not certified, this would seem to produce a } satisfactory answer. } ..Or are the regulations now so absurd that officialdom is concerned } someone may mistake brass for asbestos? } ;) } } Woody White } McDermott Technology, Inc. } http://www.mtiresearch.com/ } } http://home.att.net/~woody.white }
I would like to thank everyone who sent suggestions for cleaning the dust from a Polaroid DMC digital camera. We had great luck using a lint-free cloth moistened with methanol and very gently wiping the filter surface after opening the shutter. Practically all of the "spots" we were seeing on our digital images were successfully removed and the camera is now capable of meeting our most demanding needs. Polariod really came through with excellent technical support via the Listserver. If anyone is interested in learning more details (I know some of you mentioned you had the same "problem"), please feel free to contact me and thanks again for all your help.
Michael Simko Research Manager Metallography Group U. S. Steel Research and Technology Center
I wanted to thank everyone who sent suggestions for cleaning the dust from a Polaroid DMC digital camera. We had great luck using a lint-free cloth moistened with methanol and very gently wiping the filter surface after opening the shutter. Practically all of the "spots" we were seeing on our digital images were successfully removed and the camera is now capable of meeting our most demanding needs. Polariod really came through with excellent technical support via the Listerserver. If anyone is interested in learning more details (I know some of you mentioned you had the same "problem"), please feel free to contact me and thanks again for all your help.
Michael Simko Research Manager Metallography Group U. S. Steel Research and Technology Center
I am trying to find some information on staining mitochondria with Janus green, including the staining mechanism. However nothing relevant seems to be available in the internet or in my technical books. This is mainly for teaching purposes.
Can anyone send me information on the subject and/or relevant URL(s) to consult ?
Thanks for your help Dr. A.P. Alves de Matos apmatos-at-ip.pt
Food Structure & Functionality Symposium 2001 An international symposium leading food structure & Functionality studies through the 21st century
"webaddress at the AOCS site http://www.aocs.org/member/division/fsff/index.htm"
Being held at the 92nd AOCS Annual Meeting & Expo, May 13_16, 2001, Minneapolis Convention Center, Minneapolis, Minnesota, USA. For information, e_mail us at meetings-at-aocs.org
The symposium has two themes: * New and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure_function relationships in foods; * Food system studies covering any part of the processing chain _ from the raw material to the final product, including trouble shooting. --------------------------------------------------- Tentative Program (confirmed as of February 19th, 2001)
May 13th _ Short Courses (short courses will run for a full day, and will run concurrently)
Short Course #1 Food Contaminants. Course organiser: Mark Auty (mauty-at-moorepark.teagasc.ie)
Short Course #2 Understanding structure-function relationships in food systems through specific localisation methods and microscopy.
For more information contact Marcel Paques (paques-at-nizo.nl) ---------------------------------------------------
May 14th-16th inclusive -Technical sessions (6 sessions will run over 3 days)
Monday, May 14th, 2001 Morning Symposium opening Plenary lecture _ Food Quality and why the Structure matters P. Lillford, Unilever Research, Colworth House UK
Session 1: Dairy Products and Fat Based Foods Session _ chairs M. Auty and M. Paques
Milk protein polysaccharide interactions in aqueous solutions and oil-in-water emulsions. H. Singh*, Y. Hemar & P. Munro, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
Structural functions of dairy ingredients in products formulated with taro flour. C. Onwulata USDA/ARS,Eastern Regional Research Center,Wyndmoor, PA 19038
Confocal imaging of galactomannan mode of action in recrystallisation of ice in model ice cream D. Ferdinando, Unilever Research Colworth House, UK
Heating of Food Proteins in a Closed System at High Temperature N. Kitabatake, Kyoto University, Japan
Milk Protein -molecular components and functional properties N.C. Ganguli ,Indian Dairy Association
Afternoon Session 2: Food Safety _ chairs J. W. Arnold and R. Droleskey Prevention of Foodborne Illness Through Sanitation and Processing Technology J.W. Arnold; USDA/ARS, Russell Research Center; Athens, GA 30604
PreHarvest Intervention Strategies to Control Foodborne Pathogens in Poultry J. A. Byrd; USDA/ARS/SPARC; College Station, TX 77845
Interactions of Competitive Exclusion Cultures with the Intestinal Mucosa of Newly Hatched Chicks R. Droleskey; USDA/ARS/SPARC; College Station, TX 77845
Adhesion of Salmonella on Alfalfa Sprouts A. Chartowski; USDA/ARS, Western Regional Research Center; Albany, CA 94710
Growth of Fusarium moniliforme Dependent upon Corn Tissue Type I. E. Yates; USDA/ARS, Russell Research Center; Athens, GA 30604
Dedicated poster viewing 4:00_6:00PM
Evening: Round Table Discussion _ topic to be announced
--------------------------------------------------- Tuesday, May 15th, 2001 Morning Session 3: New Methods and Techniques for Food Structure and Functionality Analysis _chair K.Groves Applications of Scanning Near Field Optical Microscopy in the analysis of food and food-related materials. A.R. Kirby*, P. Gunning, P.J. Wilde and V. J. Morris, Institute of Food Research, Norwich, England
Diffusing wave spectroscopy - a new and non-invasive method for the investigation of the structure, dynamics and interactions in complex food systems M. Alexander* and P. Schurtenberger, Universite de Fribourg, Switzerland
AFM as a tool for characterising polysaccharides and their modification. V.J. Morris*, A.P. Gunning, A.R. Round, E. Adams, E. Kroon and G. Williamson, Institute of Food Research, Norwich, England
Micro-rheology - functional properties of food systems and their origin in microstructure M. Paques, Y. Nicolas, G. van Aken, H. Tromp, A. Janssen, Unilever Research Vlaardingen, The Netherlands
Immunolocalization of Transgenic Protein in Wheat Endosperm M. L. Parker (invited),* E. Stoger, *R. Casey and *P. Christou, Institute of Food Research, Norwich, England and *John Innes Centre, Norwich, England
Food: How complex can it be? E. Esselink ,Unilever Research Vlaardingen, The Netherlands
Multiple Extrusion Module for determining changes in consistency during working - a new method. J.F.C. Van Maanen*, B. Dunnewind, and A. Jurgens. TNO Nutrition and Food Research Institute, The Netherlands
Specific detection of components and compounds through microscopical immunodetection J. Leunissen, AURION: Immuno-Gold Reagents & Accessories, The Netherlands
Food Structure and Functionality Division Luncheon. Dr. Brian Brooker (Institute of Food Research, England, retired), the recipient of the Food Structure and Functionality Division Award, will give a presentation entitled: Fat crystals - the importance of being small.
Afternoon Session 4: Agricultural Applications of Microscopy and Imaging Session chairs D.F.Wood and P. Allan-Wojtas
The Utility of Sorting in Agriculture. H. J. Arnott, Department of Biology, University of Texas at Arlington, Texas
The Potential for Automatic X-ray Sorting of Insect Infested Grain R. Haff . USDA - ARS -WRRC, Albany, California.
Automated Sorting of Almonds with Embedded Shell by Laser Transmittance Imaging T. Pearson* R. Young, USDA -ARS- WRRC, Albany, California.
Use of a GFP-transformed strain of Fusarium graminearum to study ear rot development in corn S. S. Miller. , AAFC-ECORC, Ottawa, Ontario, Canada.
Popping modifies endosperm structure and improves digestibility in maize and sorghum. M.L. Parker, Institute of Food Research, Norwich, England.
Microstructural Changes in Rice During Cooking D. Wood* and P.C. Yu . USDA -ARS- WRRC, Albany, California.
The Food Structure and Functionality Division Member meeting will be held immediately following this session. All are welcome.
--------------------------------------------------- Wednesday, May 16th, 2001 Morning Session 5: Ingredients and Food Processing chairs D. Kittleson and J. Charbonneau
Water continuous fat crystal networks in ice cream induced by unsaturated monoglycerides N. M. Barfod , Danisco Cultor, Brabrand, Denmark
High pressure application of food systems and its impact on functional ingredients B. Tauscher*, P. Butz,and A.F. Garcia, Federal Research Center for Nutrition, Karlsruhe, Germany
Specificity and application of lipolytic enzymes in bread making processes T.B. Frandsen, T. Spendler, G. Budolfsen, L. Christiansen and J. B. Neilsen, Novozymes A/S, Bagsvaerd, Denmark
The development of bubble structure in bread doughs. M.B. Whitworth and J.M. Alava, Campden & Chorleywood Food Research Association, UK
Structure-texture relationships in heat resistant chocolate K. Groves, P. Subramaniam and O. Murphy, Leatherhead Food Research Association, UK
Reduction of oil uptake by methyl-cellulose coatings applied to fried dough M. A. Garcia, C. Ferrero, N. Bertola, M. Martino* and N. Zaritzky, CIDCA CONICET. Fac. de Ciencias Exactas and Fac. de Ingenieria, Universidad Nacional de La Plata, Argentina.
Afternoon Session 6: Colloidal and Interfacial Sciences _ chairs D. Pechak and M. Paques
Rheology and Structure of Particulate Protein Gels T. van Vliet, Wageningen Centre for Food Sciences,Wageningen, The Netherlands
Time-temperature studies of food polymer gelation. Paulson, AT., Nickerson, M.T. and Speers, R.A. Department of Food Science and TechnologyDalhousie University, Halifax, NS, Canada
--------------------------------------------------- Contact information for the chairs is shown below, in alphabetical order:
Paula Allan-Wojtas Agriculture and Agri-Food Canada Atlantic Food and Horticulture Research Centre, Kentville, Nova Scotia, Canada B4N 1J5 Tel: (902) 679-5566 FAX: (902) 679-2311 email: allanwojtasp-at-em.agr.ca
Judy Arnold USDA -ARS -RRC 950 College Station Rd. P.O. Box 5677 Athens, GA 30604-5677 USA Tel: (706) 546-3515 FAX: (706) 546-3068 email: jarnold-at-ars.usda.gov
Mark Auty Dairy Products Research Centre TEAGASC Moorepark, Femoy, Co. Cork Ireland Tel: 011-353-25-42447 FAX: 011-353-25-42340 email: mauty-at-moorepark.teagasc.ie
James E. Charbonneau National Food Processors Association Food Chemistry and Packaging Department 1401 New York Ave, NW Washington, D.C. 20005 USA Tel: (202) 639-5972 FAX: (202) 639-5991 email: jcharbo-at-nfpa-food.org
Kathy Groves Leatherhead Food Research Association Randalls Road, Leatherhead Surrey KT22 7RY England Tel: 44 0132 822330 FAX: 44 0132 386228 email: kgroves-at-lfra.co.uk
Tony McKenna New Zealand Dairy Institute Private Bag 11 029 Palmerston North, New Zealand Tel: 011 64 6 350 4649 FAX: 011 64 6 356 1476 email: tony.mckenna-at-nzdri.org.nz
Marcel Paques Wageningen Centre for Food Sciences/Unilever Research Vlaardingen P.O. Box 20, 6710 BA Ede The Netherlands Tel: 011 31 318 659690 FAX: 011 31 318 650400 email: paques-at-nizo.nl
David Pechak Kraft Technology Centre 801 Waukegan Road Glenview, IL 60025 USA Tel: (847) 646-4808 FAX: (847) 646-3864 email: dpechak-at-kraft.com
Delilah Wood USDA -ARS -WWRC 800 Buchanan Street Albany, CA 94710 USA Tel: (510) 559-5653 FAX: (510) 559-5777 email: wood-at-pw.usda.gov
Food Structure & Functionality Symposium 2001 An international symposium leading food structure & Functionality studies through the 21st century
"webaddress at the AOCS site http://www.aocs.org/member/division/fsff/index.htm"
Being held at the 92nd AOCS Annual Meeting & Expo, May 13_16, 2001, Minneapolis Convention Center, Minneapolis, Minnesota, USA. For information, e_mail us at meetings-at-aocs.org
May 13th - Short Courses (short courses will run for a full day, and will run concurrently)
Short Course #1 Food Contaminants. Most food manufacturing companies possess light microscopes, but seldom use them to their full potential. Microscopy is a powerful problem-solving tool for both contaminant analysis and food ingredient characterisation. This short course is aimed at Quality Control/Assurance and Food Product R&D personnel. The course comprises lectures and hands-on tuition from expert microscopists. The aim of the course is to provide delegates with a practical introduction to food microscopy, in particular contaminant (foreign body) identification and food ingredient characterisation.
Programme topics include: Getting the most from your light microscope - basic setting up and contrast techniques Preparing food samples for microscopy How to approach foreign body identification Identifying plant and animal remains Identifying glass, plastics, minerals Instrumental methods for contaminant analysis Microscopy in product research and development
Course contributors: John Shane (McCrone Research Institute, USA) Jim Charbonneau (National Food Processors Association, USA) Kathy Groves (Leatherhead Food Research Association, UK). Diana Kittleson (Pillsbury Technical Labs, USA) Mark Auty (Teagasc, Moorepark, Ireland) Course organiser: Mark Auty (mauty-at-moorepark.teagasc.ie)
Short Course #2 Understanding structure-function relationships in food systems through specific localisation methods and microscopy.
Spreadability, shelf life, fracture behaviour, creaminess, and mouthfeel are examples of functional properties of food products. These properties originate from the microscopic structure of the products. Specific localisation techniques and microscopy are powerful new tools to facilitate intelligent modification of ingredient composition or processing to obtain targeted product properties. The short course is aimed at R&D personnel in the Foods area (fundamental research, innovation, and product development). The course provides lectures and an intensive hands-on practical part enabling participants to gain sufficient basic knowledge and skills to set-up and implement the methods in their own work.
Programme topics include: D Introduction on specific localisation methods and principles D Localisation strategies, marking options, and imaging approaches D Experimental set-up, preparation and incubation procedures D Demonstration cases D Hands-on practical sessions
Course contributors: Jan Leunissen (Aurion: immuno Gold Reagents & accessories, custom labeling, The Netherlands) Gary Fulcher (University of Minnesota, USA) Paula Allan-Wojtas (Agriculture and Agri-Food, Canada). Marcel Paques (Wageningen Centre for Food Sciences/Unilever Research Vlaardingen, The Netherlands)
For more information contact Marcel Paques (paques-at-nizo.nl) ---------------------------------------------------
Technical sessions (6 sessions will run over 3 days) will follow the short courses from May 14th-16th inclusive.
Paula Allan-Wojtas Research Scientist - Food Microstructure Agriculture and Agri-Food Canada Atlantic Food and Horticulture Research Centre Kentville, Nova Scotia Canada B4N 1J5
I'm not sure about this, but I recall someone telling me that the current Leica eyepieces for the MZ series of stereomicroscopes will also work on the older Wild M5, M8, etc.
It would be worth a shot to call your local Leica rep, or contact Leica Customer Service at 1-800-248-0123.
My name is Hunter O'Reilly. I recently received my Ph.D. in genetics from the University of Wisconsin-Madison in December 2000. I am also a contemporary artist currently working full-time on an art project called Radioactive Biohazard. Radioactive Biohazard is a project that integrates art and science to bring awareness of issues in biotechnology to the public in a positive light. A free catalog containing scientifically accurate yet simple explanations of the artworks will be available to the public.
I am very interested in using electron micrograph images of viruses (i.e. HIV, hepatitis, rabies, polio, herpes, ebola, adenovirus) in digital artworks for the second part of this project described below. I am also interested in other visually interesting microscopic images. Any artwork that used any part of a scientist's image would give the scientist credit for that part of the image both in the exhibit and publication of the artwork. Donations of images can also be made anonymously. I have high speed internet access. Very large files can be sent to me via email.
My artwork has appeared on the covers of several international scientific journals including Nature Reviews Genetics, Medical Genetics, The EMBO Journal, Promega's Neural Notes, and Developmental Dynamics. You can view some of these covers at http://www.artbyhunter.com/media/
Please email questions and/or microscopic images to Hunter O'Reilly at
hunter-at-artbyhunter.com
You can view my artwork on my website at http://www.ArtByHunter.com
Thank you for your time and consideration! Please read further for more information.
I have received donations of images from Biotechniques and the following individuals for use in this project.
Biotechniques: A Journal of Laboratory Technology for Bioresearch and Eaton Publishing Co. Dr. Peter Angeletti, Post-Doc at the McArdle Laboratory for Cancer Research Dr. James Briscoe, Columbia University Dr. James Ellingboe, Scientific Editor of Biotechniques Dr. Prakash Hande, Columbia University Dr. Mary McCarthy, Associate Scientific Editor of Biotechniques Dr. James A. Priess, Fred Hutchinson Cancer Research Center Dr. Alison Roberts, University of Rhode Island Dr. Charlotte Schubert, formerly at the Fred Hutchinson Cancer Research Center
Radioactive Biohazard will premiere at the Walker's Point Center for the Arts in Milwaukee, Wisconsin April 20, 2001.
The exhibit will consist of three parts: 1. oil paintings reinterpreting science as art exploring themes such as cloning and genetic identity--For example I am creating one oil painting on the theme of stem-cell research, and the potential to create organs for transplants from stem-cells. I am creating another oil painting regarding the use of human cloning as a treatment for infertility. The explanations in the catalog will be simple for the general public, but scientifically accurate. Controversial issues surrounding this research will also be discussed. I will write the original explanations, and have other scientists proofread and make suggestions before they are finalized. 2. enlargements of micrographs of cells, viruses and bacteria highlighted with neon tubing--These artworks will be digital collages of microscopic images. With some of the collages, I will create a landscape. With others I may superimpose the image of a person. In the catalog and next to each artwork, there will be a diagram siting the source of each part of the image, explaining what the image is, and possibly how the image is obtained. The creation of the digital collage will be artistic, but there will be scientific explanations to accompany the works. These artworks will allow the general public to see images they have never seen before. 3. a laboratory bench installation: an artistic interpretation of a laboratory bench, equipment etc.
You will find a longer description at http://www.artbyhunter.com/artgallery/radbio.html
My homepage is at http://www.artbyhunter.com
Thank you for your time and consideration! I look forward to hearing from you!
It's a couple of years since I did any antigen retrieval and then I did it on tissue pieces, which were labelled preembedding. I found citrate buffer heated to about 95deg was effective and the most gentle. Have no info on LR White sections, except that in my hands they never seem to label with anything!
Diana
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
--
Diana van Driel Department of Clinical Ophthalmology Sydney University GPO Box 4337 Sydney NSW AUSTRALIA 2001
} From: {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com} } Look in the phone book. } } Why must we endure these mundane questions, can anybody in this world think } for themselves.
Not all of us live in a city, state or even a country that has a microscope repair and service company.
Gordon Gordon Couger gcouger-at-couger.com Stillwater, OK www.couger.com/gcouger
In some areas it may not be as simple as looking in the phone book. This listserver was meant to answer question not as a "put down' media.
Thank You,
Earl ----- Original Message ----- } From: {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com} To: {microscopy-at-sparc5.microscopy.com} Sent: Monday, February 19, 2001 3:52 PM
The question may have been unnecessary, but wasn't this response rather abrupt, unhelpful and unfriendly, particularly as it was anonymous?
} Look in the phone book. } } Why must we endure these mundane questions, can anybody in this world } think for themselves.
Have a good day.
Robin H Cross (Mr) Director : Electron Microscopy Unit Rhodes University, PO Box 94, Grahamstown, South Africa tel: +27 46 603 8168/9, fax: +27 46 622 4377 email: r.cross-at-ru.ac.za http://www.ru.ac.za/emu/index.htm
** remember ICEM-15 in Durban in September 2002 **
} } Look in the phone book. } } Why must we endure these mundane questions, can anybody in this } world think for themselves. }
Very rude.
And coming from someone sheltering behind anonymity
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
A couple of hints. Here, a local microscope maintenance company (not listed in the phone book except in yellow pages under scientific instruments) comes around once a year and is known by several other company reps. Your microscope sellers may know of someone, as may other microscopy-related suppliers, even if you can't find anything listed in the phone book or on the web. The suppliers are usually unwilling to do this type of maintenance themselves, but there are local exceptions, so it's worth asking.
good luck,
Rosemary White Microscopy Centre CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
I would like to know if anyone can recommend any books regarding the failure modes and mechanisms of plastics and ceramics. My searches have been yielded little (read as nothing), and my library is woefully lacking.
Thanks to all in advance for your help!
Chris Holp ATC Materials Lab holpcr-at-earthlink.net
Several people have already beat me to the punch, as they say, but I thoroughly agree with the rudeness of anonymity, even if it is unintentional. I have corresponded off-line with Nestor on this and my recollection is that the rules of the list preclude anonymous postings. Any one of us can occasionally make this error. But a small but annoying percentage of the members still post anonymously every time.
So why must I endure postings on asbestos when my interest is cathodoluminescence? Because it is part of the purpose of the list. And why must I endure questions from novices? Because this has also grown to be a purpose of the list and one that has been rather well served. And frankly it occasionally empowers me to ask questions that may seem stupid to those who know all things.
} Look in the phone book. } } Why must we endure these mundane questions, can anybody in this } world think for themselves. }
Donald J. Marshall Relion Industries P.O. Box 12 Bedford, MA 01730 Ph: 781-275-4695 FAX: 781-271-0252 email dmrelion-at-world.std.com
Cathodoluminescence, mass spectroscopy, electron beam technology
"A weed is a flower out of place."
(Please note: Do not send email with attachments to this address. Instead, send it to dmrelion-at-aol.com. Thank you.)
At 08:52 PM 2/19/01 -0600, Gordon Couger wrote: } } From: {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com} } } Look in the phone book. } } } } Why must we endure these mundane questions, can anybody in this } } world think for themselves. } } Not all of us live in a city, state or even a country that has a } microscope repair and service company.
Speak with respect and a humble aspect, for he is WizardOfTheLab ... -at-aol.com ! :-)
Although you were afraid to use your real name, and instead used an absurd pseudonym, allow me to enlighten you just the same. The asker of the question you have objected to was looking for a RECOMMENDATION. Facilitating the sharing of facts and opinions in a professional, helpful way is what this listserver does so well.
Perhaps you should consider unsubscribing?
Thanks, Jim
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - James F. Sanzo, Ph.D Dept. of Bioengineering 120 Hayden Hall 3320 Smith Walk University of Pennsylvania Philadelphia, PA 19104-6392
} -----Original Message----- } From: "Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com } [mailto:"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com] } Sent: Monday, February 19, 2001 5:53 PM } To: microscopy-at-sparc5.microscopy.com } Subject: Re: Cleaning and maintenance of microscopes } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Look in the phone book. } } Why must we endure these mundane questions, can anybody in } this world think } for themselves. }
I am sorry you all are taking this so terrible, I was not intending to be rude. I was merely pointing out the fact that every time someone has a slight issue they want to magnify it in this forum. I think this forum can be used for better issues than this.
It seems to me that we are all intelligent people that can find things out for ourselves. We know enough to log onto the Internet and check what is in this forum everyday. I don't see why a simple Internet search for this service cannot be performed on any search engine. I performed a search on Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in my area. We coddle people in this country like they are helpless children, we need to stop this or everybody will expect us to do everything for them.
Now I ask you do we really need this type of question in this forum?
If you all feel this is still rude I will unsubscribe.
} Now I ask you do we really need this type of question in this forum? } *** Yes. When I move to a new area, I ask colleagues to recommend a dentist; I don't merely go to the yellow pages or the web and hope for the best. In a similar vein, for most of us, our scopes are too precious to let just anyone touch them; a recommendation from a satisfied colleague is most valuable.
However, it would have been useful for the original poster to let us know where he/she was located. People from across the world subscribe to this list. A cleaning service in New Jersey may not be useful to the person in California or Australia.
Don ______________________________________________________________________ Donald L. Lovett e-mail: lovett-at-tcnj.edu Assoc. Professor, Dept. of Biology voice: (609) 771-2876 P.O. Box 7718 fax: (609) 637-5118 The College of New Jersey Ewing, NJ 08628-0718
Try Fisher Scientific or ask a nearby university, department of biology. Good luck.
} } } {"Luvstodance99-at-aol.com"-at-sparc5.microscopy.com} 02/19 1:07 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
We are searching for companies that provide cleaning and maintenance of microscopes.
These kinds of services are not in fact so easy to locate. Our yellow pages would tell you nothing useful and web searches do not provide a full list or carry any personal recommendation. The question was simple, the reply can be equally simple or you can simply delete and ignore it. The usefulness of a list like this lies in the fact that even a simple question stands a high chance of being quickly and appropriately answered, because the users have a broad range interests and experience in common. Let's not diminish this by inhibiting people from participating.
Chris
ps - who and where are you anyway?
} } I am sorry you all are taking this so terrible, I was not intending to be } rude. I was merely pointing out the fact that every time someone has a slight } issue they want to magnify it in this forum. I think this forum can be used } for better issues than this. } } It seems to me that we are all intelligent people that can find things out } for ourselves. We know enough to log onto the Internet and check what is in } this forum everyday. I don't see why a simple Internet search for this } service cannot be performed on any search engine. I performed a search on } Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in } my area. We coddle people in this country like they are helpless children, we } need to stop this or everybody will expect us to do everything for them. } } Now I ask you do we really need this type of question in this forum? } } If you all feel this is still rude I will unsubscribe. }
========================================== Dr. Chris Jeffree University of Edinburgh BIOSEM - Biological Sciences Electron Microscope Facility Institute of Cell and Molecular Biology Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 (0) 131 650 5345 FAX. #44 (0) 131 650 6563 Mobile 07710 585 401 email c.jeffree-at-ed.ac.uk =========================================
Yes, it is still rude. And it still doesn't address the issue of people who live and work outside of major centres. And you are still anonymous.
Lesley Weston.
On Tue, 20 Feb 2001 Wizardofthelab-at-aol.com-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am sorry you all are taking this so terrible, I was not intending to be } rude. I was merely pointing out the fact that every time someone has a slight } issue they want to magnify it in this forum. I think this forum can be used } for better issues than this. } } It seems to me that we are all intelligent people that can find things out } for ourselves. We know enough to log onto the Internet and check what is in } this forum everyday. I don't see why a simple Internet search for this } service cannot be performed on any search engine. I performed a search on } Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in } my area. We coddle people in this country like they are helpless children, we } need to stop this or everybody will expect us to do everything for them. } } Now I ask you do we really need this type of question in this forum? } } If you all feel this is still rude I will unsubscribe. } }
} It seems to me that we are all intelligent people that can find things out } for ourselves. We know enough to log onto the Internet and check what is in } this forum everyday. I don't see why a simple Internet search for this } service cannot be performed on any search engine. I performed a search on } Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in } my area. We coddle people in this country like they are helpless children, we } need to stop this or everybody will expect us to do everything for them. } } Now I ask you do we really need this type of question in this forum?
Perhaps you can enlighten some of the rest of us on the ease of searching. I checked the Yahoo yellow pages for "microscope cleaning" and found nothing in Iowa and not even anything around Chicago. I tried the regular Yahoo and Google searches and found thousands of matches, but I was not looking for a "coin cleaning microscope" or "cleaning a microscope slide". In view of such irrelevant results, I am not surprised that someone would ask the list for recommendations.
Perhaps you could give us a tutorial on how you were able to refine the search to produce more useful results. I consider myself rather web savvy and still fight the search engines. Such a tutorial could be a contribution rather than so much noise.
I did find the service we use for cleaning our LMs in our local yellow pages. It turns out to be through the distributor who first sold us the scopes. However, if I were not in a university town, I doubt that there would have been a listing.
} If you all feel this is still rude I will unsubscribe.
I don't know about rude, but I still have no idea of who you are. I don't know what credentials or experience you might have that I should give any consideration to your comments. An identity would help.
And in case anyone does not recognize iastate.edu as the domain for Iowa State University...
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
Chris: have you tried the ASM International website? (http://asminternational.org) Try the "Shop ASM" button at the top right to see their library of book, videos, etc. I'm looking at one now called "Plastics Failure Guide: Causes and Prevention" by Myer Ezrin. They also have lots of information on ceramics. You can also search for information. I'm a member of one of their affiliate societies, the Electronic Devices Failure Analysis Society. (You don't have to be a member to but from them, although it is cheaper.)
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-598-1291 (pager) DSPS Packaging Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
I don't think it would hurt if you identified yourself. Your mask of anonymity isn't helping matters much.
Gail Harrison Reichhold
-----Original Message----- } From: "Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com [mailto:"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com] Sent: Tuesday, February 20, 2001 11:26 AM To: microscopy-at-sparc5.microscopy.com
I am sorry you all are taking this so terrible, I was not intending to be rude. I was merely pointing out the fact that every time someone has a slight issue they want to magnify it in this forum. I think this forum can be used for better issues than this.
It seems to me that we are all intelligent people that can find things out for ourselves. We know enough to log onto the Internet and check what is in this forum everyday. I don't see why a simple Internet search for this service cannot be performed on any search engine. I performed a search on Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in
my area. We coddle people in this country like they are helpless children, we need to stop this or everybody will expect us to do everything for them.
Now I ask you do we really need this type of question in this forum?
If you all feel this is still rude I will unsubscribe.
Since you asked, yes, you were rude. No need to unsubscribe, though. Just try to show common courtesy. If you don't care about a given question, then don't reply. If you're interested in issues of self-reliant individuals and non-coddling societies, you might take your own advice and do a simple Internet search for an appropriate forum.
Vachik Hacopian
} I am sorry you all are taking this so terrible, I was not intending to be } rude. I was merely pointing out the fact that every time someone has a } slight } issue they want to magnify it in this forum. I think this forum can be } used } for better issues than this. } } It seems to me that we are all intelligent people that can find things out } for ourselves. We know enough to log onto the Internet and check what is } in } this forum everyday. I don't see why a simple Internet search for this } service cannot be performed on any search engine. I performed a search on } Yahoo and found 1000+ results. I narrowed it down to an area and found 16 } in } my area. We coddle people in this country like they are helpless children, } we } need to stop this or everybody will expect us to do everything for them. } } Now I ask you do we really need this type of question in this forum? } } If you all feel this is still rude I will unsubscribe.
Rude language and anonymity are not appropriate at all. Another point of view: ListServer is our public place for questions, answers and some "social talking". This space we have to keep clean. If for some reason you (or somebody else) don't like this place as it is- you may find many spaces on the Internet, where your language and anonymity is welcomed. Just go to the yellow pages...
} Date: Tue, 20 Feb 2001 11:25:34 -0500 (EST) } From: "Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com } Subject: Re: Cleaning and maintenance of microscopes } To: microscopy-at-sparc5.microscopy.com } X-Mailer: AOL 5.0 for Windows sub 117 } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Looks like bad timing for this question on the list, but the SEM won't wait.
Would appreciate leads on third party maintenance providers for an Amray 1830 SEM located in Sacramento, CA. This system has a Balzers 240 turbo, LaB6 gun, Varian 30L/s ion pump, load lock system, and is currently on-line and working.
Providers of service contracts for this system are sought. System is at a DOD facility.
I think this kind of questions should always be welcome. The list serve is here to entertain all kinds of microscopy related questions no matter how simple it is. I posted a similar question last year and received some excellent replies. I don't think yellow pages or internet search would have done it. We are located in a small city and I can't imagine the yellow pages will have any kind of microscopy services.
Soumitra
************************************************************* Soumitra Ghoshroy Ph.D. Electron Microscopy Lab and Fluorescence Imaging Facility Department of Biology Box 3EML New Mexico State University Las Cruces, NM 88003 Tel: 505-646-3600 Fax: 505-646-5665 e-mail:sghoshro-at-nmsu.edu http://confocal.nmsu.edu/eml
The whole idea of this listserver is to synergistically combine the expertise of people from a variety of backgrounds, locations, and experience levels so we can assist each other. I suggest you rejoin those of us who (usually) quietly lurk until you realize that flaming is neither helpful nor appropriate.
The person who requested assistance was thinking for herself - and doing it well. After all microscopists are the best source of information on individuals/companies truly qualified to clean microscopes. I do standard cleaning/maintenance on my PLM's and stereomicroscopes at work and have cleaned up many low end 'scopes as a freebie for public schools. But I wouldn't dream of fully disassembling/cleaning some of the high end microscope systems I've used, even if I had the tools available. I'd want a true expert - not just a name I pulled from the yellow pages or internet.
Louise Harner Research Microscopist Albany International Research Co. 777 West Street, P.O. Box 9114 Mansfield, MA 02048 508-339-7300 phone 508-339-4996 fax Louise_Harner-at-albint.com
"Wizardofthela b-at-aol.com" To: microscopy-at-sparc5.microscopy.com cc: 2001/02/19 Subject: Re: Cleaning and maintenance of microscopes 06:52 PM
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Look in the phone book.
Why must we endure these mundane questions, can anybody in this world think for themselves.
I'm an engineer in Nikon Japan. I used to work as sales engineer in NY. How old and which maker's microscope do you mention? If it is Nikon, could you contact our service or dealer? I know our service department clean and maintain microscopes with reasonable charge. Please contact 516-845-7788 first.
Hisashi Okugawa 1st design department Instrument company in Nikon Corporation Phone: 81-45-853-8568 Fax: 81-45-853-8475 e-mail: okugawa.h-at-nikon.co.jp ----- Original Message ----- } From: {"Luvstodance99-at-aol.com"-at-sparc5.microscopy.com} To: {Microscopy-at-sparc5.microscopy.com} Sent: Tuesday, February 20, 2001 3:07 AM
No Great Loss.
Earl
----- Original Message ----- } From: {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com} To: {microscopy-at-sparc5.microscopy.com} Sent: Tuesday, February 20, 2001 2:08 PM
I am hesitant to reply to such a message, a few list members have expressed much of my sentiments, but think that there is a deeper problem with a few (very few but vocal) members of this list. I realize I am preaching to the choir and those that should hear, won't. Attacks such as this need to be discouraged.
Because of the quickness of a very few to attack without provocation, usually my answers are restricted to off the list responses. This gets the information to the inquirer, without requiring e-mail after e-mail in some sort of battle. There have been useful lively conversations that I learned much from, and gained deeper understanding, but these have always remained civil, and dealt with the question at hand. Responses such as below, really stifle I want from the list as a list member, a free flow of questions and answers. These are what I learn from. There is a delete key or trash bin for what I don't want to read, doesn't take long, nor much effort. I have an enormous file of those I keep for future reference and things I didn't know. The list works!
This list is for questions, hopefully for which, the inquirer doesn't readily have the answer. What is to one, mundane and trivial, to others is a significant question. Who is to chose what is mundane and for that matter what is the scope of the inquiry? When read, I understood that advice was being sought for the quality of service as well as who. This is the kind of information that is available through this list. I am sorry I wasn't able to respond to the original question but it was not from my region, so I can't suggest good service engineers in the inquirer's area to help.
I for one, would NOT want to trust a research light microscope worth $30,000.00 or more to a choice from the Yellow pages (nor the Web for that matter) without further information. We all have thrown bad service personnel out of the lab, and had to pay the price of having the job done right later. There is no information in the Yellow pages on how good or how bad a given company is. Users of these services DO have this information and we are the users.
As you send an e-mail, ask yourself, if the response you are giving doesn't answer, or some way relate to, the question, why are you sending it, and what good will it do for fellow list members?
Remember: There are no stupid questions, only stupid answers.
At 06:52 PM 2/19/2001 EST, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Well, I thought I'd see if I, too, could find 1000+ microscope maintenance companies in the US, and there are a few out there. Search under microscope maintenance:
www.southernmicro.com in Georgia southernmicroscope.com/services.html somewhere in the South www.dscoptical.com/services.htm near Boston www.caleywhitmore.com near Boston www.opticalexpertise.com in New York www.mwrn.com/product/microscope/repair.htm lists various service companies www.sciscope.com/field-service.htm based in Iowa, service most states www.svms.com/services/index.html in silicon valley www.meyerinst.com/html/mts/mts.htm in Texas www.microresource.com/services2.htm in Illinois www.allometrics.com/microscope_serv.htm in Lousiana and Texas www.labworksinc.com in San Fransisco www.biotherm-inc.com in 5 southern states www.mikroni.com/fp/fp/services.htm in San Diego www.uams.edu/oas/OES/oesilab.htm in Arkansas www.uni.edu/pur/maintenance/equipment.html Uni of Northern Iowa www.dominionmicroscope.com/services.htm Richmond, VA
etc.
took an hour to weed through them, while staining some sections......
cheers,
Rosemary White Microscopy Centre CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
"I would like to know if anyone can recommend any books regarding the failure modes and mechanisms of plastics and ceramics. My searches have been yielded little (read as nothing), and my library is woefully lacking.
Thanks to all in advance for your help!"
Chris Holp ATC Materials Lab holpcr-at-earthlink.net
I have been working with SEM since the beginning of the 80's and one of my literature companions during all these years has been the excellent book "An Atlas of Polymer Damage", ISBN 0 7234 0750 9. Regarding ceramics I have little experience and do not know any good source.
Best regards
Pierre Frykberg Alfa Laval Tumba AB Div. Materials
Relax, and welcome to a public (though moderated) forum.
You got flamed because you failed to realize the extent of this list. I've been a denizen for many years and have found questions and responses on every level of expertise. As an internet resource, this list is exceptional; however, you have to be responsible for your own filtering as to what responses you make.
Basically, those posting here are interested primarily in constructive information. Had you requested the original poster's location and provided them with the results of your searches located within their area, there would have been nothing left to argue. You would have been lauded as helpful and the poster would have had the help they requested.
No whiney children here, only people of a wide variety of different abilities and experience in this narrow field of knowledge who hope to find some deeper understanding and practical help in fulfilling the tasks they have to pursue each day.
I'm sorry if you really do leave this list. Having been in this field for over 20 years, I rather enjoy the infusion of newcomers.
As an independent businessman, I am a staunch republican and arch-rival of 'political correctness', but I see none in this thread. A simple question was asked, requiring a simple answer. If you didn't have that simple answer, you should have just remained silent and deleted the email. Instead, you chose to inject your displeasure that this particular email was sent. Here's a surprise - this list is not maintained for your pleasure, but for the practical use of hundreds, if not thousands, of others.
You are welcome here, at least by me (I certainly can't speak for anyone else here). Please recognize that you are preaching to the masses here, and not just to an audience that you desire.
On Tuesday, February 20, 2001 4:08 PM, "Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com [SMTP:"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com] wrote: } } I am going to make you all happy and unsubscribe. You are making this exactly } what I was hoping to avoid. } } You all sound like a bunch of whiny children. } } Political correctness is ruining the fiber of this great country. } } Enjoy your pathetic lives. } } Mike Nolan } President } Materialographic Technologies } } P.S. I am using a back-up ISP while my Primary ISP is working out some } problems and I did not realize my signature was not included. } } }
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174 voice 630.513.7093 fax 630.513.7092
With regard to the recent "point / counter point" thread regarding the rude postings of "the Wiz", I was initially reluctant to "add to the pile" when so many had already voiced many of my sentiments. For years, I have encouraged my SEM students at a local university to subscribe to "the List" and regularly read and learn from the problems and questions many people have shared with List members. If the students had specific questions or similar problems, I have further encouraged them to post their comments or questions. The most common response from students is "I don't DARE post my silly question because everyone else will know the answer and I will look foolish. I don't want to be put down or scolded before thousands of knowledgeable readers" (I might add, who does?!) Until this recent thread from "the Wiz", I could comfortably assure my students the list was a place for good information without the assault of self appointed experts like "the Wiz". The true damage a rude person like "the Wiz" does is to discourage others from responding eventually leading to the death of the list server.
Problems like this one are inevitable. A few years ago, a forensic list server was plagued with an even more blatant and as you would guess, anonymous flamer. The flamer took delight in ridiculing many list members and seeing how far he/she/they could upset the list members. The list response was much the same as we are experiencing with "the Wiz". Our words will not beat him/her/they back and their offer to unsubscribe is a joke. Like a bully on a playground, if everyone ignores him/her, they will loose interest and go away. If he/she/they cannot master to ability to delete unwanted questions or text, how can we possibly expect them to abide by the unpublished rules of good manners and mutual respect?
When I have learned EVERYTHING about ALL types of microscopy, I may see "the Wizard's" view. But until then, I will enjoy and learn from fellow microscopists and their responses to the MSA List server. Let's not waste more personal (or company) resources on "the Wiz".
Keep up the good work, Nestor!
OBLIGATORY DISCLAIMER: My comments and opinions expressed in the above text are mine alone and do not represent those of the US FDA or any other Federal agency.
S. Frank Platek Forensic Chemistry Center U.S. Food and Drug Administration 6751 Steger Drive Cincinnati, OH 45237-3097 (513) 679-2700 X254 fplatek-at-ora.fda.gov
} From: "Allen R. Sampson" {ars-at-sem.com} Send reply to: "ars-at-sem.com" {ars-at-sem.com} To: "'\"Wizardofthelab-at-aol.com\"-at-sparc5.microscopy.com'" {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com} , "microscopy-at-sparc5.microscopy.com" {microscopy-at-sparc5.microscopy.com}
Hi Listers,
Nestor, would you please call a halt to all the flying insults?
Everybody is getting their panties in a knot over this and I think it should end. Wizard was rude, but the replies are rude to.
To quote a "friend" of the LAPD ;-), "Can't we all just get along?"
Paula :-)
Paula Sicurello George Washington Univ. Medical Center Dept. of Pathology, Ross Hall rm 505 Electron Microscope Lab 2300 Eye St. Washington, DC 20037 202-994-2930 phone 202-994-2518 fax
More years ago than I want to mention, whenever we changed the filament on a Siemens 102 we cleaner the ceramic insulator with some sort of polish to remove any C buildup. Does anyone have a suggestion what to use as a "polish" for this.
------------------------------------------------------- Laurence Marks Department of Materials Science and Engineering Northwestern University Tel: (847) 491-3996 Fax: (847) 491-7820 mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu -------------------------------------------------------
Workshop May 17-19 2001 "New approaches to the Phase Problem" http://xraysweb.lbl.gov/esg/phasing/index.html
by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id JAA13196 for dist-Microscopy; Wed, 21 Feb 2001 09:16:46 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id JAA13192 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Wed, 21 Feb 2001 09:16:16 -0600 (CST) Received: from e1g1.home.nyu.edu (E1G0.HOME.NYU.EDU [128.122.108.150]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id JAA13181 for {microscopy-at-sparc5.microscopy.com} ; Wed, 21 Feb 2001 09:16:05 -0600 (CST) Received: from kal-s-quantex (mcs01-ext.med.nyu.edu [128.122.2.2]) by e1g1.home.nyu.edu (8.10.1/8.10.1) with ESMTP id f1LFEJt20359 for {microscopy-at-sparc5.microscopy.com} ; Wed, 21 Feb 2001 10:14:19 -0500 (EST) Message-Id: {4.2.0.58.20010221101117.009544a0-at-imap.nyu.edu} X-Sender: kr4-at-imap.nyu.edu X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58
} I'm not sure about this, but I recall someone telling me that the current } Leica eyepieces for the MZ series of stereomicroscopes will also work on the } older Wild M5, M8, etc.
Now that a little of the invective has quieted down, here's the contact information for John Sowers, who services Amenex's light microscopes from his location in Claymont, Delaware:
J&L Microscope Services, (302) 798-5304
John has been servicing our B&L and Olympus microscopes for about fifteen years; they still work as well or better than they did when he started. What more can I say ?
Tell John I put you on to him.
Best regards, George Langford, Sc.D. Principal Consultant Amenex Associates, Inc. amenex-at-amenex.com http://www.amenex.com/
If it is a glazed insulator then we find that alumina (Al2O3) powder mixed with alcohol to form a slurry is good. Dipping a cotton bud in the slurry and working at the mark, then a general clean all around the component with the slurry on wipes is very effective. The alumina is easy to clean off and does not leave any residue. It is very important to wash thoroughly with frequent changes of alcohol.
If the tracking is very bad (deep) then it may be necessary to shot blast with alumina and then wash in alcohol. Shot blasting also works for some of the porous insulators.
Good luck, Ron
On Wed, 21 Feb 2001 07:27:08 -0600 (CST) "L. D. Marks" {ldm-at-risc4.numis.nwu.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } More years ago than I want to mention, whenever we changed } the filament on a Siemens 102 we cleaner the ceramic insulator } with some sort of polish to remove any C buildup. Does anyone } have a suggestion what to use as a "polish" for this. } } ------------------------------------------------------- } Laurence Marks } Department of Materials Science and Engineering } Northwestern University } Tel: (847) 491-3996 Fax: (847) 491-7820 } mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu } ------------------------------------------------------- } } Workshop May 17-19 2001 "New approaches to the Phase Problem" } http://xraysweb.lbl.gov/esg/phasing/index.html } }
---------------------- Mr. R.C. Doole Department of Materials, University of Oxford. Parks Road, Oxford. OX1 3PH. UK. Phone +44 (0) 1865 273701 Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
The bottom line is that "The List" should not be blamed for the opinion of "The Wiz". Here in America we advocate free speech, and we should be used to the idea that unpopular opinions will therefore occasionally be expressed -- and we shouldn't overreact to them. "The List" is an open forum, and so "The Wiz" has a perfect right to express his opinion. But students and beginners should realize that most of us don't share that opinion in this case, and they should feel perfectly free to ask questions about anything they find confusing in the vast realm of microscopy. It doesn't make any sense to me that anyone would abandon ship (unsuscribe) just because an unpopular opinion was expressed. We need collectively to have "thicker skins".
Kent Christensen
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A. Kent Christensen, Professor Emeritus Department of Cell and Developmental Biology, Medical Science II Building University of Michigan Medical School, Ann Arbor, MI 48109-0616 Office: 5801 Medical Science II Building Tel (work) (734) 763-1287, Fax (work) (734) 763-1166 E-mail: akc-at-umich.edu ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
--On Wed, Feb 21, 2001 6:38 AM -0500 "Platek, Frank" {FPLATEK-at-ora.fda.gov} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } With regard to the recent "point / counter point" thread regarding the } rude postings of "the Wiz", I was initially reluctant to "add to the } pile" when so many had already voiced many of my sentiments. For years, } I have encouraged my SEM students at a local university to subscribe to } "the List" and regularly read and learn from the problems and questions } many people have shared with List members. If the students had specific } questions or similar problems, I have further encouraged them to post } their comments or questions. The most common response from students is } "I don't DARE post my silly question because everyone else will know the } answer and I will look foolish. I don't want to be put down or scolded } before thousands of knowledgeable readers" (I might add, who does?!) } Until this recent thread from "the Wiz", I could comfortably assure my } students the list was a place for good information without the assault of } self appointed experts like "the Wiz". The true damage a rude person } like "the Wiz" does is to discourage others from responding eventually } leading to the death of the list server. } } Problems like this one are inevitable. A few years ago, a forensic list } server was plagued with an even more blatant and as you would guess, } anonymous flamer. The flamer took delight in ridiculing many list members } and seeing how far he/she/they could upset the list members. The list } response was much the same as we are experiencing with "the Wiz". Our } words will not beat him/her/they back and their offer to unsubscribe is a } joke. Like a bully on a playground, if everyone ignores him/her, they } will loose interest and go away. If he/she/they cannot master to ability } to delete unwanted questions or text, how can we possibly expect them to } abide by the unpublished rules of good manners and mutual respect? } } When I have learned EVERYTHING about ALL types of microscopy, I may see } "the Wizard's" view. But until then, I will enjoy and learn from fellow } microscopists and their responses to the MSA List server. Let's not waste } more personal (or company) resources on "the Wiz". } } Keep up the good work, Nestor! } } OBLIGATORY DISCLAIMER: } My comments and opinions expressed in the above text are mine alone and do } not represent those of the US FDA or any other Federal agency. } } } S. Frank Platek } Forensic Chemistry Center } U.S. Food and Drug Administration } 6751 Steger Drive } Cincinnati, OH 45237-3097 } (513) 679-2700 X254 } fplatek-at-ora.fda.gov }
At 03:03 AM 2/21/01, you wrote: } I don't know if it will help, but here's the response I had to make earlier } today to a DOD request in the Sacramento area. The email address I was } given didn't work, so I will have to call the party tomorrow. He claimed } that they had three Amrays at their location. Amray's new service policies } have certainly created some problems for customers!
Are any of you aware of a change in Amray's (KLA-Tencor) service policies? It seems that their main problem is lack of trained personnel. The second driver is the diminishing of importance of the lab SEMs versus the KLA semiconductor fab/inspection equipment.
The problem with this particular 1830 system is that pre-contract inspection is done on a per-diem basis. This is the lowest priority service call. Systems are not placed on contract until the pre-inspection is done. Sort of a Catch-22 situation.
This may not be a huge problem for systems like a 1000 or 1600 or 1610T. Maybe not too bad for an 1830. Non-Amray service ought to be fine. But for FESEMs like 1840, 1845, 1880, 1910, 3300 and 3600, it is quite another matter. Is there an impression or actual set of data points where Amray/KLA is dropping the non-FESEMs and retaining the FESEMs or simply dropping all models of SEMs?
KLA-Tencor, Amray Division recently sent me a notice they were no longer providing service contracts for my area (Michigan). They also sent a list of eleven third party service providers. We contracted with one of those vendors for our service (Ex-Amray guys of course). The vendor we chose simply accepted the instrument without inspection since we were just coming off contract with KLA.
I can't speak for FESEMs since ours is an 1820 LaB6.
Good luck with your search.
Kevin
--------------------------------------------------------------- Kevin Battjes Senior Research Specialist Michigan Molecular Institute Voice 517-832-5555, ext 556 1910 W. St Andrews Road Fax 517-832-5560 Midland MI 48640 e-mail: battjes-at-mmi.org After April 6, 2001 use 989 area code ---------------------------------------------------------------
-----Original Message----- } From: Gary Gaugler [mailto:gary-at-gaugler.com] Sent: Wednesday, February 21, 2001 11:37 AM To: ars-at-sem.com Cc: MSA listserver
At 03:03 AM 2/21/01, you wrote: } I don't know if it will help, but here's the response I had to make earlier } today to a DOD request in the Sacramento area. The email address I was } given didn't work, so I will have to call the party tomorrow. He claimed } that they had three Amrays at their location. Amray's new service policies } have certainly created some problems for customers!
Are any of you aware of a change in Amray's (KLA-Tencor) service policies? It seems that their main problem is lack of trained personnel. The second driver is the diminishing of importance of the lab SEMs versus the KLA semiconductor fab/inspection equipment.
The problem with this particular 1830 system is that pre-contract inspection is done on a per-diem basis. This is the lowest priority service call. Systems are not placed on contract until the pre-inspection is done. Sort of a Catch-22 situation.
This may not be a huge problem for systems like a 1000 or 1600 or 1610T. Maybe not too bad for an 1830. Non-Amray service ought to be fine. But for FESEMs like 1840, 1845, 1880, 1910, 3300 and 3600, it is quite another matter. Is there an impression or actual set of data points where Amray/KLA is dropping the non-FESEMs and retaining the FESEMs or simply dropping all models of SEMs?
To my knowledge KLA Tencor got out of the lab SEM business to include service. They did not renew our service contract and supplied a list of third party vendors . This is where I got Grant Gerringer's name.
I would suggest you contact AMRAY directly and get the list of third party vendors and a copy of the letter.
If it is a glazed insulator then we find that alumina (Al2O3) powder mixed with alcohol to form a slurry is good. Dipping a cotton bud in the slurry and working at the mark, then a general clean all around the component with the slurry on wipes is very effective. The alumina is easy to clean off and does not leave any residue. It is very important to wash thoroughly with frequent changes of alcohol.
If the tracking is very bad (deep) then it may be necessary to shot blast with alumina and then wash in alcohol. Shot blasting also works for some of the porous insulators.
Dear Laurence,
If the shot-blasting is necessary, you may want to check out the Air Eraser, available from Scientific Instrument Services. It's not expensive and can operate on valved-down house compressed air. It has a small tip, so it can easily be controlled.
Look at this dialogue as "theater-a la-internet" and we can see this in many different perspectives. Social commentary? Have we abdicated our ability to understand more than one perspective? It makes for great life art. I like to see it as an aggravation, a lesson, and humor....being in the audience...but then I love EM as art!
While we are expressing our feelings, let me strongly suggest that subscribers temporarily unsubscribe if they are going to use an autoanswer reply when out of the office.
If one posts to this list, one is addressing the entire list and a stream of autoanswer SPAM should not result. Frankly, my dears, I don't like getting autoanswer messages from people I do not know informing me how long they will be away.
There was an earlier posting requesting information and leads for x-ray inspection systems for integrated circuits. I must have lost the post but I have found a recent lead for what seems like a nice system. It is quoted in the $75K price range as was at the requested budget amount in the original posting.
I hate to be the bearer of bad news to the list but I feel many people will want to know this. After a long battle with cancer Terry Donovan passed away yesterday. While his primary association with electron microscopy was as a salesman he nonetheless had a vigorous enthusiasm for the field and promoted it where ever he went. No doubt that over the years he touched the lives of many people in a positive way, as he did mine. Anyone who knew he was a sailing enthusiast may be interested to know he wanted his ashes put into the Chesapeake Bay. Regards........Alan Berginc Cressington Scientific, Inc 508 Thomson Park Drive Cranberry Township, PA 16066-6425 TEL 724-772-0220 (USA)
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I am relatively new to this listserve and have been quietly monitoring the various threads and wide array of responses. Although much has no relevance to my work, I have found many responses quite useful and thank the list for the opportunities it provides.
I am passing along a request for recommendations on image analysis software. A client has a collection of fluorescence micrographs (film originals) that they would like to analyze quantitatively. They do not have a digital capture system that allows them to do the quantitation real time. I believe it is a simple matter of thresholding and measurimg the areas, but I may be missing something in my assumptions.
I have already recommended NIH (Scion) Image for the purpose, as I have used an early version of that software for similar purposes, but they would like to know if there are any highly recommended commercial packages that might be better, or if there are any companies that could do this analysis for them (again from their film originals).
The request comes from a graduate student at the University of Illinois in Chicago who has money for this purpose.
Any suggestions either through the list or to me personally are greatly appreciated and I will forward those responses on.
Thanks for your time.
James Hayden
*********************************
James E. Hayden, RBP, FBCA Bio-Graphics 1058 Hemlock Drive Blue Bell, PA 19422 tel/fax: (215)654-0625 tel:(215)514-4223
by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id SAA15133 for dist-Microscopy; Wed, 21 Feb 2001 18:22:58 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id SAA15130 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Wed, 21 Feb 2001 18:22:28 -0600 (CST) Received: from mta6.snfc21.pbi.net (mta6.snfc21.pbi.net [206.13.28.240]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id SAA15123 for {Microscopy-at-sparc5.microscopy.com} ; Wed, 21 Feb 2001 18:22:17 -0600 (CST) Received: from house ([63.205.136.150]) by mta6.snfc21.pbi.net (Sun Internet Mail Server sims.3.5.2000.01.05.12.18.p9) with SMTP id {0G94004XHTXGXT-at-mta6.snfc21.pbi.net} for Microscopy-at-sparc5.microscopy.com; Wed, 21 Feb 2001 16:12:54 -0800 (PST)
At 02:55 PM 2/21/01, you wrote:
(snip) } This may not be a huge problem for systems like a 1000 or 1600 } or 1610T. Maybe not too bad for an 1830. Non-Amray service } ought to be fine. But for FESEMs like 1840, 1845, 1880, 1910, } 3300 and 3600, it is quite another matter. Is there an impression } or actual set of data points where Amray/KLA is dropping the non-FESEMs } and retaining the FESEMs or simply dropping all models of SEMs?
The official word from Amray is that they are supporting the FESEM at this time & will do so "for probably another five years". As I do not have that in writing, the "five year" policy may change.
} Are any of you aware of a change in Amray's (KLA-Tencor) service } policies? It seems that their main problem is lack of trained personnel. } The second driver is the diminishing of importance of the lab SEMs } versus the KLA semiconductor fab/inspection equipment. } } The problem with this particular 1830 system is that pre-contract inspection is } done on a per-diem basis. This is the lowest priority service call. } Systems are not placed on contract until the pre-inspection is done. } Sort of a Catch-22 situation.
I don't understand. Are you trying to obtain a service contract from Amray at this time? Or are you trying to obtain a contract from a "third party' maintenance organization?
Regards,
Earl
----- Original Message ----- } From: "Gary Gaugler" {gary-at-gaugler.com} To: {ars-at-sem.com} Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, February 21, 2001 8:37 AM
They do exist.
You might try "Aspex" (formerly RJ Lee Instruments) at (724) 744-0100.
Earl
----- Original Message ----- } From: "rad0" {rden25-at-mindspring.com} To: "microscopers!!" {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, February 21, 2001 11:06 AM
I am fairly sure this is the case. I had a Leica MZ8 and MZ6 and the eyepieces could be interchanged with the Wild M8 I had. I am not sure about tube lengths and all that if such things are critical on stereo microscopes.
I have recently been approached by some of our researchers with the request to assist them in developing an imaging system that enables them to measure the thickness of a liquid and/or vapour film that develops when water is sprayed onto a hot metal surface.
In approaching me with their request they hoped that my experience in electron microcopy might give them some ideas. However, I am at a loss.
Can anyone out there give me advise whom to contact and what technique has been developed to measure (in situ) liquid film thicknesses in the order of approximately 30 microns.
Thanks
Hans Brinkies Senior Lecturer Swinburne, University of Technology School of Engineering and Science P.O.Box 218 - Hawthorn - Vic -3122 - Australia Phone: +61 3 9214 8657 Fax: +61 3 9214 8264 Email: Hbrinkies-at-swin.edu.au
Optical techniques might work. Ellipsometry or other reflection techniques. Contact someone at JA Woollam in Lincoln, Nebraska.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
-----Original Message----- From: Hans Brinkies [mailto:HBrinkies-at-groupwise.swin.edu.au] Sent: Wednesday, February 21, 2001 8:33 PM To: microscopy-at-sparc5.microscopy.com Subject: Liquid film thicknesses
--------------------------------------------------------------- --------- The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html
I have recently been approached by some of our researchers with the request to assist them in developing an imaging system that enables them to measure the thickness of a liquid and/or vapour film that develops when water is sprayed onto a hot metal surface.
In approaching me with their request they hoped that my experience in electron microcopy might give them some ideas. However, I am at a loss.
Can anyone out there give me advise whom to contact and what technique has been developed to measure (in situ) liquid film thicknesses in the order of approximately 30 microns.
Thanks
Hans Brinkies Senior Lecturer Swinburne, University of Technology School of Engineering and Science P.O.Box 218 - Hawthorn - Vic -3122 - Australia Phone: +61 3 9214 8657 Fax: +61 3 9214 8264 Email: Hbrinkies-at-swin.edu.au
I've been getting a lot of inquiries from Amray customers for contract proposals, including one for a 3300 FESEM. In that case, I don't think that Amray made any gestures - they also have a 1600 series SEM and are not impressed with the service on the 3300 so we are talking about servicing both.
Don't be too alarmed. Some of us independents are the most experienced EM service personnel out there. I have over twenty years as an independent working on all EM manufacturers as well as a wide variety of other instruments such as XRF, AAS, UV-Vis, GC-MS, IR-FFT and a variety of microprobes, not to mention my previous experience with an SEM manufacturer, IBM and others.
The major concern for an FESEM owner transferring to an independent is, of course, the cathode assembly. Amray will charge $10,000 for a swap and billable installation on top of that. Denka, however, manufactures emitters that will work in most FESEMs for generally less than $3,000 and are available through Energy Beam Sciences. While there are increased demands in reconditioning the electrostatic surfaces in an FESEM cathode, they are easily managed. Given today's manufacturers service contract pricing, you should be able to find an independent coming in at nearly 50% less, not including the emitter replacements. Add in the purchase of one emitter per year, and your costs should still be considerably lower, while the experience level of your service engineer is increased.
Many FESEMs sold are located in companies that also have clean room facilities or laminar flow hoods. Clean rooms only certify a certain elimination of airborne particulates. While that is useful, it only addresses one part of the contamination concerns regarding FE cathodes - one that can be easily handled through a careful and methodical cathode assembly under nearly any conditions.
The other concern is that of chemical cleanliness of the lens surfaces. Once again, use of appropriate cleaning and handling methods can produce good results. There is nothing magical here, more than likely the manufacturers are doing the same work under conditions that may surprise you, not to mention the experience and qualifications of the employees doing the work.
Like a good jeweler or machinist, anyone experienced in servicing these machines should be able to eyeball dimensions down to 1/10,000 of an inch, perhaps with the help of a loupe. Alignment of any critical components shouldn't be any problem.
Don't be shy to ask the questions you have of any independent you may be considering. We survive on our integrity in a very limited field. If an independent can't properly handle customer questions and problems, they won't be able to feed their families for long. Believe me, it's tough to keep a business like this going if you can't at least equal the service capabilities of the manufacturer, as low a bar as that may be at times.
I can't tell you what Amray is considering other than the announcements they have already made. I can tell you that similar moves were considered by ISI prior to their withdrawal from the American market and subsequent marketing and service licensing through another manufacturer. While such a move is more than unlikely for Amray, I'd say that SEM manufacturers in general are looking for creative ways to get out of self-imposed difficulties.
It is possible that we are witnessing yet another new trend and other manufacturers may be considering similar moves. The SEM field has grown greatly over the past two decades, and manufacturers still seem unable to recognize the true value of their service organizations - that of marketing support by helping to build a base of loyal customers.
Instead, they are increasingly demanding their service organizations be independent profit centers. In doing so, they bring a bean-counter mentality to service that minimizes the concept of a career for service engineers. By doing so, they encourage a rapid turn-over in their service force which is why you probably have seen either a never ending stream of promising, but inexperienced, engineers or a civil-service stereotypical engineer who never quite makes the grade, but never leaves.
Sorry to have indulged in yet another of my tirades on the manufacturers. Now and then there appears one manufacturer who seems to get it, and they can profit greatly. There is one, who shall remain un-named, that I recommended to my customers for over a decade because of the post sales service they provided. I'm probably responsible for well over a million dollars a year in sales for them for five to ten years.
I saw the writing on the wall when they announced lay-offs at their corporate offices over five years ago; in a country where such a thing was unheard of at the time. Their response seems to have been a clamp down on expenditures and rising prices in foreign countries, witnessed by the increasing requests I've received for service on their instruments over the last few years. Frankly, I would have preferred to see them continue their monopoly on good service, as I only give advice I am reasonable sure of. However, having said that, I can state to everyone, as I stated in the advice that I have given in the past, that every manufacturer eventually f___s up.
Putting on the head dress of soothsayer, I can speculate that manufacturers are looking to increase their profits by dumping those lines that produce the least profit and concentrate on those lines that produce the most. Having everyone who has bought into the latest and greatest of technological advances, i.e. FE, snuckered into thinking that there are no alternatives to their magic, there are greater profits for the manufacturers in the service of these instruments. After all, the alternative would seem to be a nearly doubling of their already bloated service costs.
The trend over the last two decades has been to introduce new product models at a rapid rate, regardless of any real evolutionary improvements. These are due primarily to the increased influence of marketing departments that seek not just to distance themselves from the competition, but also from their own offerings. In this trend, manufacturers have screwed themselves as the learning curve for inexperienced service personnel for a large variety of models is much longer than is really necessary. The result is that they expect more from their service engineers and place increasing demands on them while not providing increased incentives. I've yet to see a service engineer promoted beyond service manager. Yet human resource, marketing and accounting underlings are commonly promoted to top corporate positions. Why stay in service?
I've seen the industry standard service contract cost rise from 5% of purchase price per year to over 10%. I have not seen any indication that those costs are justified, except through poor management decisions by the manufacturers. Since the purchase prices themselves reflect any basic cost increases over the years, exactly how do you justify the doubling of service costs which I've stated in proportion to the purchase price?
Have I given you a hint of where I'm coming from? Sorry, but sometimes when you give me a nail, I'll grab a 16 pound sledge hammer, although never when I'm working on an SEM. However, you may want to bolt your windows, as I often want to toss an instrument out. (To Fred, and anyone else who tends to take me too seriously, the immediately preceding was intended as a joke, as poor as it was).
On Wednesday, February 21, 2001 10:37 AM, Gary Gaugler [SMTP:gary-at-gaugler.com] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } At 03:03 AM 2/21/01, you wrote: } } I don't know if it will help, but here's the response I had to make earlier } } today to a DOD request in the Sacramento area. The email address I was } } given didn't work, so I will have to call the party tomorrow. He claimed } } that they had three Amrays at their location. Amray's new service policies } } have certainly created some problems for customers! } } Are any of you aware of a change in Amray's (KLA-Tencor) service } policies? It seems that their main problem is lack of trained personnel. } The second driver is the diminishing of importance of the lab SEMs } versus the KLA semiconductor fab/inspection equipment. } } The problem with this particular 1830 system is that pre-contract inspection is } done on a per-diem basis. This is the lowest priority service call. } Systems are not placed on contract until the pre-inspection is done. } Sort of a Catch-22 situation. } } This may not be a huge problem for systems like a 1000 or 1600 } or 1610T. Maybe not too bad for an 1830. Non-Amray service } ought to be fine. But for FESEMs like 1840, 1845, 1880, 1910, } 3300 and 3600, it is quite another matter. Is there an impression } or actual set of data points where Amray/KLA is dropping the non-FESEMs } and retaining the FESEMs or simply dropping all models of SEMs? } } Thanks, } gary gaugler } } }
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174 voice 630.513.7093 fax 630.513.7092
There are quite a few ISI-40s still in use. As a basic SEM, it works. ISI made their inroads in the American market as a low bidder, offering a useable instrument at a price that others couldn't match.
There are a variety of independent service providers out there that can service your instrument, but you need to specify your location.
The last I knew, ISI made their schematics available at an incredibly low cost (I got mine at $35 US). Access to ISI is currently through Leo (have I got it right?) and you should contact their parts department for the schematics. Their schematics are in Japanese (or is it Korean?) so you should be cognizant of international electronic symbols and nomenclature. If not, then you should seek the help of someone who is. As far as I am aware, the electrical schematics are all that is available.
On Wednesday, February 21, 2001 1:06 PM, rad0 [SMTP:rden25-at-mindspring.com] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, } } I have aquired the remnants of an ISI-40 sem. } } I'm new to this thing, and I'm going to need some sort } of technical manual just to identify the components. } } So, do these exist? Did they ever? } } And, if so, where might I start looking for them? } } And also, this thing looks mighty old, is this model still used } by anyone, and are there technicians still alive who could work } on it? } } Thanks, } Rick } } }
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174 voice 630.513.7093 fax 630.513.7092
I would like to announce the 1st International Meeting & Workshop on Advanced Light Microscopy - June 6-10, 2001 - Santa Maria Imbaro, Italy.
The program will include lectures on recent exciting advances in the fields of light microscopy and their applications to modern questions in life sciences. The practical workshop (afternoon), organised by manufacturers, leading in the field, will give the possibility to learn practical aspects on the latest developments in the field. Evening session will give students the possibility to discuss their own work and we also will have round table discussions bringing together manufacturers and scientists discussing where light microscopy presently stands and where it should move to in the future.
For further information, please check this web page: http://www.embl-heidelberg.de/ELMI/ItalyMeeting
Having dug further into my emails for the day, Earl has provided the right answer, contrary to my previous posting. I swear, its getting harder every day to track the passage of individual SEM manufacturers. RJ Lee did indeed handle the ISI assets in the US, my mistake, and you should contact them for info. The transfer to Aspex was not known to me, but web links to RJ Lee as a manufacturer appear to be non-existent and Earl is probably right. I'm confused...
On Wednesday, February 21, 2001 1:06 PM, rad0 [SMTP:rden25-at-mindspring.com] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, } } I have aquired the remnants of an ISI-40 sem. } } I'm new to this thing, and I'm going to need some sort } of technical manual just to identify the components. } } So, do these exist? Did they ever? } } And, if so, where might I start looking for them? } } And also, this thing looks mighty old, is this model still used } by anyone, and are there technicians still alive who could work } on it? } } Thanks, } Rick } } }
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174 voice 630.513.7093 fax 630.513.7092
Thank you all for the myriad of responses to the inquiry regarding failures in plastics and ceramics. Below is a brief compilation of suggested sources:
"Medical Plastics: Degradation Resistance & Failure Analysis", Portnoy, ISBN 1-884207-60-X "Plastics Failure Guide: Cause & Prevention", Ezrin, ISBN 1-56990-184-8 (These two are available at the www.asm-intl.org site)
"An Atlas of Polymer Damage" ISBN 0-7234-0750-9 "Failure of Plastics", Brostow & Corneliussen, ISBN 3-446-14199-5 "Polymer Microscopy" ISBN 0-412-25710-6 "An Atlas of Polymer Damage", Engle et al, ISBN 0-13-050013-5 "Polymer Degradation", W. Schnabel "Case Studies of Plastics Design & Failure Analysis", Mallick/Ford Motor Co. "Handbook of Plastics Technology", SPE "Polymer Characterization & Analysis", Kroschwitz "Handbook of Plastics Flammability", Landrock "Handbook of Plastics Degradation", Mamid & Amin
This is a quick list and I have not yet investigated each title, but I do have a few on order. I was actually impressed at the number of relevant to semi-relevant books shown at Amazon.com. Also, The Society of Plastics Engineers has a nice site with good book offerings.
Chris Holp ATC Materials Lab Cleveland, OH holpcr-at-earthlink.net
Access is through Aspex. Leo is a consortium of Zeiss & Cambridge.
----- Original Message ----- } From: "Allen R. Sampson" {ars-at-sem.com} To: "'rad0'" {rden25-at-mindspring.com} ; "microscopers!!" {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, February 22, 2001 1:42 AM
Dear Laurence, I have used six micron diamond polishing paste for this, in the past. It must be very thoroughly washed off with clean ethanol or methanol. At 07:27 AM 2/21/01 -0600, you wrote: } More years ago than I want to mention, whenever we changed } the filament on a Siemens 102 we cleaner the ceramic insulator } with some sort of polish to remove any C buildup. Does anyone } have a suggestion what to use as a "polish" for this. } } ------------------------------------------------------- } Laurence Marks Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
I'm having a stabiliy problem with LR White sections. They curl, expand, split, etc. during examination on the TEM. My operating conditions are 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity low. In the past I've tried vac. evap. a layer of carbon which helped some. I was also thinking of coating the grids with another layer of formvar to "sandwich" the sections. I would appreciate anyone's thoughts and what they have tried in solving the stability problem. thanks.
We've had nothing but trouble with LR White and have finally switched to Unicryl to see if that improves things. Not only has LRW been unstable under the beam, but we've had repeated problems with sections being destroyed in the immunolabeling and staining processes. We have cleaned and recleaned our grids. We have tried different hardness grades of LRW and different batches of resin. We've tried extended dehydrations and infiltrations and abbreviated ones, from the manufacturer's suggested protocol to every sensible variation we could think of. The final straw was when we processed LRW and Unicryl embedded samples side by side and got beautiful stable sections in Unicryl and nonexistent to tattered shreds on the LRW grids.
We're still pretty new to Unicryl, so I'm sure it will have its own set of problems, but so far it's been more than acceptable.
There are some archived discussions about LR resins at http://www.biotech.ufl.edu/~emcl/tips.html.
Meanwhile, I'd be interested in seeing any responses you get, if you wouldn't mind.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
-----Original Message----- } From: Tom W Bargar [mailto:tbargar-at-unmc.edu] Sent: Thursday, February 22, 2001 1:12 PM To: Microscopy-at-sparc5.microscopy.com
I'm having a stabiliy problem with LR White sections. They curl, expand, split, etc. during examination on the TEM. My operating conditions are 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity low. In the past I've tried vac. evap. a layer of carbon which helped some. I was also thinking of coating the grids with another layer of formvar to "sandwich" the sections. I would appreciate anyone's thoughts and what they have tried in solving the stability problem. thanks.
I'm trying to track down a formulation for Teorell and Stenhagen buffer. A 2.5mM concentration of diaminobenzidine is used with this buffer and hydrogen peroxide to selectively stain the catalase in peroxisomes. The references that I have found all seem to use this buffer and reference an article in a German journal dating to 1938. Is anyone out there familiar with this buffer? If so, can you supp;y the recipe? Is there another buffer that I can use to effectively stain peroxisomes? As always, thanks in advance for your replies.
Tom Januszewski Senior Electron Microscopist UT Southwestern Medical Center at Dallas Dallas, TX 75390-9039 214-648-7291 FAX: 214-648-6408 Email: tom.januszewski-at-UTSouthwestern.edu
Tom - Have you tried sort of "burning" the sections at low mag, low beam for a few minutes when they first go in the beam? That may help stabilize them for work at higher mags. That has helped me in the past.
However, I'm joining the Unicryl camp :) Never had trouble with losing LR White sections during processing, but the few times I've used Unicryl so far I've had less trouble with microholes in the sections (had those in LR Gold, too) and the sections have been more stable in the beam.
Not bashing LR resins - they've worked just fine for me for years, but I'm all for trying newer stuff when I can!
Tamara
On Thu, 22 Feb 2001, Tom W Bargar wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I'm having a stabiliy problem with LR White sections. They curl, expand, } split, etc. during examination on the TEM. My operating conditions are } 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity } low. In the past I've tried vac. evap. a layer of carbon which helped some. } I was also thinking of coating the grids with another layer of formvar to } "sandwich" the sections. I would appreciate anyone's thoughts and what they } have tried in solving the stability problem. thanks. } } Tom Bargar } EM Lab, UNMC } Omaha, NE } (402)559-7347 } } }
|--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward-at-unm.edu |--------------------------------------------------|
Tom, I use routinely formvar/carbon coated copper grids. The other way of support I use, especially for high resolution work and elemental analysis where I don't want to look through the layer of carbon, is a holey "lacey" carbon film. The support is superb, the drawback is a limited viewing area. I purchase both from EMS. I've never tried to make a sandwich with another formvar layer, but sounds to me kind of labor-intensive (not speaking of another layer you'd be imaging through). Good luck, Alice.
Alice Dohnalkova Battelle, PNNL MS P7-50 Richland, WA 99352 (509) 372-0692
-----Original Message----- } From: Tom W Bargar [mailto:tbargar-at-unmc.edu] Sent: Thursday, February 22, 2001 11:12 AM To: Microscopy-at-sparc5.microscopy.com
I'm having a stabiliy problem with LR White sections. They curl, expand, split, etc. during examination on the TEM. My operating conditions are 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity low. In the past I've tried vac. evap. a layer of carbon which helped some. I was also thinking of coating the grids with another layer of formvar to "sandwich" the sections. I would appreciate anyone's thoughts and what they have tried in solving the stability problem. thanks.
RJ Lee Group had spun off RJ Lee Instruments a couple of years ago. RJ Lee Instruments, Limited changed it's name to Aspex, LLC last year. ASPEX stands for Application Specific Products using Electron beam and X-ray analytical technologioes, they tell me. It was easier for me to remember RJ Lee. For more info their web address is www.aspexllc.com . Which has some contact information or a button for questions as I recall. If ASPEX doesn't handle what you want I'm sure they can give you contact information for RJ Lee Group.
Jim Roberts
James L. Roberts Firearm and Toolmark Examiner Ventura Co. Sheriff's Lab (805) 654-2308 James.Roberts-at-mail.co.ventura.ca.us
} } } "Allen R. Sampson" {ars-at-sem.com} 02/22/01 02:13AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Having dug further into my emails for the day, Earl has provided the right answer, contrary to my previous posting. I swear, its getting harder every day to track the passage of individual SEM manufacturers. RJ Lee did indeed handle the ISI assets in the US, my mistake, and you should contact them for info. The transfer to Aspex was not known to me, but web links to RJ Lee as a manufacturer appear to be non-existent and Earl is probably right. I'm confused...
On Wednesday, February 21, 2001 1:06 PM, rad0 [SMTP:rden25-at-mindspring.com] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, } } I have aquired the remnants of an ISI-40 sem. } } I'm new to this thing, and I'm going to need some sort } of technical manual just to identify the components. } } So, do these exist? Did they ever? } } And, if so, where might I start looking for them? } } And also, this thing looks mighty old, is this model still used } by anyone, and are there technicians still alive who could work } on it? } } Thanks, } Rick } } }
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174 voice 630.513.7093 fax 630.513.7092
Below is the result of your feedback form. It was submitted by (muller.bruce-at-columbus.co.za) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday, February 22, 2001 at 06:28:29 ---------------------------------------------------------------------------
Email: muller.bruce-at-columbus.co.za Name: Bruce Muller
State: Mpumalanga
Zip: 1050
Question: What solution and method would one use to deep-etch Type 316 STAINLESS STEEL to reveal the inclusions and/or precipitates present in relief. This will be used for SEM analysis. Thankyou in advance for your assistance. Bruce
Hi I think there are a few things you should be careful with. First, don't use naked grids with LRW sections, always use film supported grids. Second, don't try to use chloroform to extend the wrinkles of your sections before picking them up. Third, don't use UAc in EtOH or MtOH, use aquatic UAc for your staining.
Good luck
Greg Ning EM Facility Medical College of Wisconsin
Tom W Bargar wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I'm having a stabiliy problem with LR White sections. They curl, expand, } split, etc. during examination on the TEM. My operating conditions are } 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity } low. In the past I've tried vac. evap. a layer of carbon which helped some. } I was also thinking of coating the grids with another layer of formvar to } "sandwich" the sections. I would appreciate anyone's thoughts and what they } have tried in solving the stability problem. thanks. } } Tom Bargar } EM Lab, UNMC } Omaha, NE } (402)559-7347
Hello, Can someone please give me a source for free or inexpensive Si wafer rejects? I break them into small squares to use as SEM substrates for nano particles. Thank you, Jim
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility Unit-2131 Storrs, CT 06269-2131 bsgphy3-at-uconnvm.uconn.edu 860 486-2914 voice 860 486-1936 fax
The company that now owns ISI is the Optical company TOPCON they have branches through out the world and locally Topcon still has "ISI" instruments available. Regards JVN
"Allen R. Sampson" wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } There are quite a few ISI-40s still in use. As a basic SEM, it works. ISI } made their inroads in the American market as a low bidder, offering a } useable instrument at a price that others couldn't match. } } There are a variety of independent service providers out there that can } service your instrument, but you need to specify your location. } } The last I knew, ISI made their schematics available at an incredibly low } cost (I got mine at $35 US). Access to ISI is currently through Leo (have } I got it right?) and you should contact their parts department for the } schematics. Their schematics are in Japanese (or is it Korean?) so you } should be cognizant of international electronic symbols and nomenclature. } If not, then you should seek the help of someone who is. As far as I am } aware, the electrical schematics are all that is available. } } On Wednesday, February 21, 2001 1:06 PM, rad0 [SMTP:rden25-at-mindspring.com] } wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hello, } } } } I have aquired the remnants of an ISI-40 sem. } } } } I'm new to this thing, and I'm going to need some sort } } of technical manual just to identify the components. } } } } So, do these exist? Did they ever? } } } } And, if so, where might I start looking for them? } } } } And also, this thing looks mighty old, is this model still used } } by anyone, and are there technicians still alive who could work } } on it? } } } } Thanks, } } Rick } } } } } } } } Allen R. Sampson, Owner } Advanced Research Systems } 317 North 4th. Street } St. Charles, Illinois 60174 } voice 630.513.7093 fax 630.513.7092
-- John Nailon Operations Manager The Centre for Microscopy and Microanlaysis The University of Queensland St Lucia QLD 4072 Tel: +61-7-33654214 Fax: +61-7-33654422 WWW: http://www.uq.edu.au/nanoworld
I would pick up sections on the carbon-plastic-coated grid and then put second layer of carbon over. In such "sandwich", extra couple of nanometers of carbon does not harm your resolution. Carbon should work perfectly to protect your sections. Secondly, I would try higher HT, like 80 kV.
Good luck, Sergey
At 11:12 AM 2/22/01 -0800, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
A few years ago I did quite a bit of immunogold labelling on LR White sections on uncoated 400 mesh copper thin-bar grids (washed thoroughly with acetone) at 80-100 kV and imaging at 40-100K. I usually "baked" the sections a bit at low mag before imaging at high mag - as suggested by Tamara Howard. They were rather unstable, but I would usually be able to count gold particles and/or take a photo before the section blew up. As you say, keeping the beam intensity low prolonged their life a bit, enough to get the information needed. So I didn't solve the problem, but just worked around it as much as possible. Also, I cut thickish sections - dark gold, expanding to pale gold with chloroform vapour waved over the knife bath. Longer polymerisation might help, but then you may lose antibody access to the tissue, if that's what you're interested in. I used medium or hard grade resin polymerised ~16 hours at 55 C. Over 4 years, we did have a couple of dud batches that didn't polymerise very well.
good luck, Rosemary White } } I'm having a stabiliy problem with LR White sections. They curl, expand, } split, etc. during examination on the TEM. My operating conditions are } 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity } low. In the past I've tried vac. evap. a layer of carbon which helped some. } I was also thinking of coating the grids with another layer of formvar to } "sandwich" the sections. I would appreciate anyone's thoughts and what they } have tried in solving the stability problem. thanks. } } Tom Bargar } EM Lab, UNMC } Omaha, NE } (402)559-7347
Rosemary White Microscopy Centre CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
The objectives of our Leitz DURIMET Micro-Hardness tester was damaged during class experiments last year. Has anyone an idea where I can purchase a x10 (A 0.18 C HM25~) and a x40 (A 0.7 C HM6.3~) objective that suits a Leitz DURIMET.
Thanks
Hans Brinkies Senior Lecturer Swinburne, University of Technology School of Engineering and Science P.O.Box 218 - Hawthorn - Vic -3122 - Australia Phone: +61 3 9214 8657 Fax: +61 3 9214 8264 Email: Hbrinkies-at-swin.edu.au
{HTML} {BODY} {!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2//EN"} {HTML}
{HEAD} {SCRIPT LANGUAGE=3D"JavaScript1.1"}
{!-- Begin function right(e)
{
var msg =3D "Not Available";
if (navigator.appName =3D=3D 'Netscape' && e.which =3D=3D 3)
{
alert(msg); // Delete this line to disable but not alert user
return false;
}
else
if (navigator.appName =3D=3D 'Microsoft Internet Explorer' && event.button= =3D=3D2)
{
alert(msg); // Delete this line to disable but not alert user
return false;
}
return true;
} var mclick =3D 0; function cy(form) { var mthanks =3D 'Thank You For Your Interest'; var mclick =3D mclick+1; var remmsg =3D "Error! Missing Data."; if (!form.removeemail.value) { var remmsg =3D" Error! Please enter an email address"; alert(remmsg); return; } else { form.submit(); return; }
} // End of function validate_remove
function cyorder(form) { var x=3D1; var ordermsg =3D "Error! Missing Data";
if (!form.Phone.value) { var ordermsg =3D "Error! Please enter your phone number."; x=3D2; } if (!form.State.value) { var ordermsg =3D "Error! Please enter your state."; x=3D2; } if (!form.City.value){ var ordermsg =3D "Error! Please enter your city."; x=3D2; } if (!form.Country.value){ var ordermsg =3D "Error! Please enter your country."; x=3D2; } if (!form.Name.value){ var ordermsg =3D "Error! Please enter your name."; x=3D2; } if (x=3D=3D2) { alert(ordermsg); return false; }else{ form.submit(); return true; }
} // End of function validate_remove
document.onmousedown =3D right;
// End --}
{/SCRIPT}
{META NAME=3D"GENERATOR" Content=3D"Visual Page 1.0 for Windows"} {META HTTP-EQUIV=3D"Content-Type" CONTENT=3D"text/html;CHARSET=3Diso-8859= -1"} {TITLE} This Automatic Money Magnet is Incredibley Easy 8215 {/TITLE} {/HEAD}
{BODY BGCOLOR=3D"#FFFFFF"}
{P} {TABLE BORDER=3D"0" WIDTH=3D"553"} {TR} {TD WIDTH=3D"481"} {P} {FORM ACTION=3D"mailto:getitdone2001-at-underwriters.com?subject=3DPlea= seCallandSendVideo" METHOD=3D"POST" ENCTYPE=3D"text/plain"} {FONT COLOR=3D"#FF0000"} {B} FREE DOW JONES INVESTMENT VIDEO! {BR} {/B} {/FONT} {FONT COLOR=3D"#0033FF"} {B} Build the future you want, with t= he index you can trust! {BR} {/B} {/FONT} {FONT COLOR=3D"#000000"} {BR} Are you looking to protect yourself from a market correction {BR} or to protect your overall portfolio? {B} Invest in something people {BR} USE EVERYDAY {/B} and will continue to use everyday... {B} COMMODITIES. {= /B} {BR} {BR} {B} Don't just invest in stocks {/B} , diversify your portfolio with futur= es {BR} and futures options and accomplish your investment objectives! {BR} There has never been a better time, and the correct information {BR} has never been more readily available! Just look at the information {BR= } you can find on the internet! {BR} {BR} People were making money with commodities long before the internet {BR} was even invented! {/FONT} {FONT COLOR=3D"#0033FF"} {B} This an inexpensiv= e alternative to "just stock" {BR} ownership that can improve your overall portfolio by participating {BR} in a broader worldwide market . {/B} {/FONT} {FONT COLOR=3D"#000000"} {BR} {BR} Can you imagine, for just one day, how people would react {BR} worldwide if they thought could not have their first cup of {BR} coffee or even some orange juice in the morning? {BR} {/FONT} {FONT COLOR=3D"#DD0000"} {B} What would happen to prices? {BR} {/B} {/FONT} {FONT COLOR=3D"#000000"} {BR} {B} Do you watch the news and pay attention to the signs around you? {BR= } {/B} At just a hint of a cold front, heating oil prices jump. {BR} With just a hint of a frost in Florida or California, {BR} orange prices jump. With just a hint of a hurricane heading {BR} our way, plywood prices jump. Every year as vacation season {BR} begins, gasoline prices jump. {BR} {BR} {B} This is the world of supply and demand...BUY LOW and SELL HIGH! {/B} = {BR} Do you remember when sugar prices went through the roof? {BR} A lot of early investors in sugar made absolute fortunes! {BR} {BR} Commodities are used by people in literally every country {BR} in the world. Such things as gasoline, heating oil, coffee, {BR} sugar, corn, wheat, soybeans, apples, oranges, fish, beef, {BR} and pork, are some of the more well known. You've probably {BR} even heard of the infamous Pork Bellies! Let us show you {BR} how you can participate and profit in this lucrative market {BR} in the the new millenium! {BR} {BR} {B} Examples: {BR} {/B} 1) Due to oversupplies, combined the current lack of Asian demand, {= BR} {B} the prices of corn wheat, and soybeans are at their LOWEST {BR} prices in 20 years! {/B} However, with a significant drought in some maj= or grain {BR} growing areas and early signs of economic recovery in Asia, grain {BR} prices could increase substantailly soon. Learn how to speculate in {BR} the grain markets by leveraging 25,000 bushels of corn, wheat, or soybe= ans! {BR} {BR} 2) You can also profit NOW from today's high gas prices! {BR} On a $5000 investment you can control over 420,000 gallons {BR} of gasoline by using options. {B} Just a 5 cent movement in your favor {B= R} means thousands of dollars of profit for you! {/B} {BR} {BR} "Oil, a century old commodity, is behaving like an upstart {BR} internet company." Wall Street Journal, April 2000 {BR} {BR} Just ask Bill Lakewoth in Florida who said... {BR} "I clicked and {B} made $95,000 in less than 90 days! {/B} {BR} Boy, do I like those Oil Markets!" {BR} {BR} We are a small independent USA firm that really does cater to our clien= ts. {BR} We have 25 years of experience and our results are second to none. {BR} Learn how our clients get the best results and receive the most {BR} professional care in the investment world today. {BR} {BR} {/FONT} {FONT COLOR=3D"#0033FF"} {B} You have nothing to lose and everythi= ng to gain! {/B} {/FONT} {FONT COLOR=3D"#000000"} {BR} {BR} {/FONT} {FONT COLOR=3D"#FF0000"} {B} Get your FREE VIDEO NOW! {/B} {/FONT} {= FONT COLOR=3D"#000000"} {BR} {BR} {B} Just type in the following required information and we {BR} will call you back to confirm your request! {/B} {BR} {/FONT} {BR}
{TABLE BORDER=3D"0" WIDTH=3D"89%"} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} Full Name {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"Name" SIZE=3D"29" MAXL= ENGTH=3D"35"} {/TD} {TD WIDTH=3D"16%"} {FONT COLOR=3D"#FF0000"} Required {/FONT} {/TD} {/TR} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} Address {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"Address" SIZE=3D"29" M= AXLENGTH=3D"35"} {/TD} {TD WIDTH=3D"16%"} {/TD} {/TR} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} City {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"City" SIZE=3D"29" MAXL= ENGTH=3D"35"} {/TD} {TD WIDTH=3D"16%"} {FONT COLOR=3D"#FF0000"} Required {/FONT} {/TD} {/TR} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} State {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"State" SIZE=3D"29" MAX= LENGTH=3D"35"} {/TD} {TD WIDTH=3D"16%"} {FONT COLOR=3D"#FF0000"} Required {/FONT} {/TD} {/TR} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} Country {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"Country" SIZE=3D"29" M= AXLENGTH=3D"35"} {/TD} {TD WIDTH=3D"16%"} {FONT COLOR=3D"#FF0000"} Required {/FONT} {/TD} {/TR} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} Zip Code {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"Zip" SIZE=3D"14" MAXLE= NGTH=3D"14"} {/TD} {TD WIDTH=3D"16%"} {/TD} {/TR} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} Phone Number {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"Phone" SIZE=3D"25" MAX= LENGTH=3D"35"} {/TD} {TD WIDTH=3D"16%"} {FONT COLOR=3D"#FF0000"} Required {/FONT} {/TD} {/TR} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} Best Time To Contact You {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"Best_Time" SIZE=3D"25"= MAXLENGTH=3D"35"} {/TD} {TD WIDTH=3D"16%"} {/TD} {/TR} {TR} {TD WIDTH=3D"36%"} {P ALIGN=3D"RIGHT"} E-Mail {/TD} {TD WIDTH=3D"48%"} {INPUT TYPE=3D"TEXT" NAME=3D"Email" SIZE=3D"25" MAX= LENGTH=3D"35"} {/TD} {TD WIDTH=3D"16%"} {/TD} {/TR} {/TABLE} {INPUT TYPE=3D"HIDDEN" NAME=3D"Subject" SIZE=3D"-1" VALUE=3D"Auth for Dow = Jones"} {A NAME=3D"order"} {/A} {/P} {P ALIGN=3D"CENTER"} {B} Please click only once it may take up to 30 seco= nds. {/B} {/P} {CENTER} {P} {INPUT TYPE=3D"SUBMIT" NAME=3D"Submit" VALUE=3D"I Authorize" onClick= =3D"cyorder(this.form)"} {/P} {/CENTER} {P} {FONT COLOR=3D"#000000"} **The Information submitted is treated as st= rictly confidential {BR} and will be used for program package verification only. {BR} {BR} *There is a significant risk of loss in all commodity trading. {BR} Only risk capital should be used. Carefully consider your {BR} financial position before trading. {/FONT} {/P}
{P} {FONT COLOR=3D"#000000"} {B} {BR} (REMOVAL INSTRUCTIONS) {BR} {/B} This mailing is done by an independent marketing company. {BR} Please do not use the reply to this e-mail, an e-mail {BR} reply cannot be read! If you would like to be removed from {BR} our mailing list, just click below and send us a remove request email. {= BR} {BR} {/FONT} {BR} {B} Please click only once it may take up to 30 seconds. {/B} {BR} {/FORM} {FORM ACTION=3D"mailto:notme750-at-europe.com?subject=3DRemoveDowVideoPromo" = METHOD=3D"POST" ENCTYPE=3D"text/plain"} {INPUT TYPE=3D"HIDDEN" NAME=3D"RemoveIt" SIZE=3D"-1" VALUE=3D"Remove My Email = } From Any Future Dow Jones Mailings" ."} {TABLE BORDER=3D"0" WIDTH=3D"398" BGCOLOR=3D"#DDDDDD"} {TR} {TD WIDTH=3D"100%" HEIGHT=3D"44" VALIGN=3D"TOP"} {INPUT TYPE=3D"TEXT" = NAME=3D"removeemail" SIZE=3D"34"} {INPUT TYPE=3D"SUBMIT" NAME=3D"Submit" VA= LUE=3D"Remove" onClick=3D"cy(this.form)"} {/TD} {/TR} {/TABLE}
Depending on what you call cheap, Ted Pella sells 4" wafers precut to either 5x7 or 5x5mm chips for $61.00 US.
Dale
On Thu, 22 Feb 2001, JIM ROMANOW wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, } Can someone please give me a source for free or inexpensive Si wafer } rejects? I break them into small squares to use as SEM substrates for nano } particles. } Thank you, } Jim } } James S. Romanow } The University of Connecticut } Physiology and Neurobiology Department } Electron Microscopy Facility } Unit-2131 } Storrs, CT 06269-2131 } bsgphy3-at-uconnvm.uconn.edu } 860 486-2914 voice } 860 486-1936 fax } } }
Dale Shumaker G9 WBSB 725 N Wolfe Street Baltimore, MD 21205
Wilson Lab; Johns Hopkins University School of Medicine, Cell Biology and Anatomy 410-614-2654 410-955-4129 (fax)
Hi Tom! LR-White behaves sometimes a little bit "excentric" ... I would like to give you some hints drawn from my personal experience with LR-White:
-I use medium grade LR-W. That´s not because I tried other grades, but this works well with me
- The way of LRW polymerization is critical. If you do it in the heat, at 55-60°C, with or without vacuum, plastic gets very hard and sometimes brittle. Although, it´s easy to cut but the sections tend to be unstable in the beam, they suffer badly during immunoprocedures (sometimes only 1 out of 4 survives...), they are destroyed by alcoholic UA, contrast is sometimes awfully low and so on ...
- I polymerize LR-White in the cold at -20° by addition of 15microlitres of accelerator to 1ml of fresh resin which is in good-sealing microtubes (air prevents polymerization). What I get is not that good to cut because is softer than heat-polymerized LR-W, but it comes off the knife without wrinkles, is very stable during processing and in the beam at even 80kV. Contrast is sufficient, even if you only stain with (aquaeous) Uranyl and omit the lead. Immunolabeling works fine if you add 0.025 % Tween-20 to washing buffer and secondary gold-probe.
- Yes its true, the older this stuff gets even in your fridge, the poorer polymerization (especially by the use of the accelerator) will be. One year should be a batch´s life more or less.
- I use simply formvar coated nickle/copper grids. That is sufficient. If you have the facilities to coat the grids with carbon, it might work even better, everything is more robust during immunolabeling.
Why don´t you try it with the accelerator. Important: be quick as polymerization sets on immeadiatly, for that cool down the resin together with the samples (-20°C), don´t polymerize more than a handful of samples at one time. Use some kind of plastic microtubes (eppendorf, sarstedt and so on ...) which seal tight! After adding accelerator to the cold resin, shake vigorously and than hurry up. Make a paper copy of sample numbers and use them, laser-printouts get smeary and unreadable after LRW polymerziation.
If you have further questions, don´t hesitate to contact me.
Good luck, Michael
Michael Reiner University of Cologne, Germany Dept. of Anatomy I
} I'm having a stabiliy problem with LR White sections. They curl, expand, } split, etc. during examination on the TEM. My operating conditions are } 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity } low. In the past I've tried vac. evap. a layer of carbon which helped some. } I was also thinking of coating the grids with another layer of formvar to } "sandwich" the sections. I would appreciate anyone's thoughts and what they } have tried in solving the stability problem. thanks. } } Tom Bargar } EM Lab, UNMC } Omaha, NE } (402)559-7347
Have you tried making carbon/metal grids (Collodion or Forvar removed). I'm wondering if the metal might help dissipate the heat. Additionally, can you increase your HT (even though the potential energy is higher) which will decrease wavelength which I have found helps with beam sensitive materials. Although, maybe you require low KV for contrast.
I have been using Pella's precut wafers for making cross section samples. I only use them as blanks to build up the cross section. They come with an adhesive sheet that binds them together. When I get further into the middle of the wafer, the edges of the piece have a tendency to chip because of the adhesive. Does anyone know how to release them without this occurring. I am concerned about solvents contaminating them and preventing adhesion.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
-----Original Message----- From: Dale Shumaker [mailto:dshumake-at-mail.jhmi.edu] Sent: Friday, February 23, 2001 12:49 AM To: JIM ROMANOW Cc: microscopy-at-sparc5.microscopy.com Subject: Re: Si wafers wanted
--------------------------------------------------------------- --------- The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html --------------------------------------------------------------- --------.
Depending on what you call cheap, Ted Pella sells 4" wafers precut to either 5x7 or 5x5mm chips for $61.00 US.
Dale
On Thu, 22 Feb 2001, JIM ROMANOW wrote:
} --------------------------------------------------------------- --------- } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html }
--------------------------------------------------------------- --------. } } } Hello, } Can someone please give me a source for free or inexpensive Si wafer } rejects? I break them into small squares to use as SEM substrates for nano } particles. } Thank you, } Jim } } James S. Romanow } The University of Connecticut } Physiology and Neurobiology Department } Electron Microscopy Facility } Unit-2131 } Storrs, CT 06269-2131 } bsgphy3-at-uconnvm.uconn.edu } 860 486-2914 voice } 860 486-1936 fax } } }
Dale Shumaker G9 WBSB 725 N Wolfe Street Baltimore, MD 21205
Wilson Lab; Johns Hopkins University School of Medicine, Cell Biology and Anatomy 410-614-2654 410-955-4129 (fax)
we appreciate very much all responses about our request related to a negative analysis of asbestos in building materials. We still might ask some of you "off line" for details or further comments. Thanks a lot!
Silvia Montoro Centro Regional de Investigaciones y Desarrollo de Santa Fe Santa fe Argentina
Try: Roels, F, et al: J. Histochem. Cytochem. 22:442-4,1974 Roels, F, et al: J. Histochem. Cytochem. 27: 1471-7, 1979 Roels, F, et al: Am. J. Med. Genet. 25:257-71, 1986
One of these might have the recipe, however, I have used a procedure published by De Craemer, et al in the Journal of Histochemistry and Cytochemistry, 17:675-680, 1969. They use Tetra-HCl buffer which is more commonly called Tris-HCl buffer. I labeled catalase in liver peroxisomes. I immersion fixed 2mm thick sections of liver 6 hours in 3% Glut made up in 0.1M Cacodylate buffer, then vibratomed 50 um tissue slabs. I used 5-20mg DAB(10mg is good) diluted in 0.1M Tris-HCl buffered to 9.0 pH, add 0.02% H2O2, then incubate 30-60 (you can extend to 120) min at 37C. Rinse in Tris buffer and post-fix in glut, osmicate, dehydrate and embedd in epoxy resin. This was in normal rat liver and the label was good. Make sure you are using really fresh H2O2. Some of the older published articles have EM pictures with very dark label, but these days you should expect less DAB intensity. I have talked to a biochemistry/liver researcher about the decrease in intensity of DAB label, and she was certain that the changes were do to modern manufactured food supplements which are different from what was fed to rats back in the 60's and 70's. One last thing about liver peroxisomes, they are more numerous in the centrolobular areas of the liver.
Good Luck!
} ---------- } From: Tom Januszewski[SMTP:tom.januszewski-at-email.swmed.edu] } Reply To: tom.januszewski-at-email.swmed.edu } Sent: Thursday, February 22, 2001 1:39 PM } To: microscopy-at-msa.microscopy.com } Subject: Teorell-StenhagenBuffer } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi Listservers, } } I'm trying to track down a } formulation for Teorell and } Stenhagen buffer. A 2.5mM } concentration of diaminobenzidine } is used with this buffer and } hydrogen peroxide to selectively } stain the catalase in peroxisomes. } The references that I have found } all seem to use this buffer and } reference an article in a German } journal dating to 1938. } Is anyone out there familiar with } this buffer? If so, can you supp;y } the recipe? Is there another buffer } that I can use to effectively stain peroxisomes? } As always, thanks in advance for } your replies. } } Tom Januszewski } Senior Electron Microscopist } UT Southwestern Medical Center at Dallas } Dallas, TX 75390-9039 } 214-648-7291 } FAX: 214-648-6408 } Email: tom.januszewski-at-UTSouthwestern.edu }
I have to correct a mistake, I realized as soon as I sent the e-mail response.......I'm sorry, I have a really bad cold and I mistakenly said there was a change in DAB labeling intensity, which is not what I wanted to say!!
The change is in the number of peroxisomes present in the rat liver due to the dietary changes from the 60's to present day.
} ---------- } From: Tom Januszewski[SMTP:tom.januszewski-at-email.swmed.edu] } Reply To: tom.januszewski-at-email.swmed.edu } Sent: Thursday, February 22, 2001 1:39 PM } To: microscopy-at-msa.microscopy.com } Subject: Teorell-StenhagenBuffer } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi Listservers, } } I'm trying to track down a } formulation for Teorell and } Stenhagen buffer. A 2.5mM } concentration of diaminobenzidine } is used with this buffer and } hydrogen peroxide to selectively } stain the catalase in peroxisomes. } The references that I have found } all seem to use this buffer and } reference an article in a German } journal dating to 1938. } Is anyone out there familiar with } this buffer? If so, can you supp;y } the recipe? Is there another buffer } that I can use to effectively stain peroxisomes? } As always, thanks in advance for } your replies. } } Tom Januszewski } Senior Electron Microscopist } UT Southwestern Medical Center at Dallas } Dallas, TX 75390-9039 } 214-648-7291 } FAX: 214-648-6408 } Email: tom.januszewski-at-UTSouthwestern.edu }
We have a question regarding a trouble shooting of chatter/compression on brain sections.
The vibratome we used is VIBRATOME 3000, automated with refrigeration, the mouse brain tissue was fixed in 4% paraformaldehyde, the thickness of section cut was 30-50 um. We have being adjusting either speed or amplitude, however, it doesn't seem to solve the problem.
We would appreciate anyone who would kindly help us to solve this problem.
Many thinks.
Xinran
*********************************************************** Xinran Liu, M.D., Ph.D. Center for Basic Neuroscience UT Southwestern Medical Center 6000 Harry Hines Blvd., NA4. 214A Dallas, TX 75390-9111
I would like to know if there are training courses anywhere in North America on the statistics of quantitative microscopy or stereology. If so, where, or where can I search? Pmiller-at-mbt.com
Dear Bruce, I have not done this etch myself, but my copy of Van der Voort has an etch specifically for SEM of stainless steels: "5 ml Acetic acid 5 ml HNO3 15 ml HCl Aqua regia plus acetic acid. For ferritic grades (of stainless steel). Swab sample 15 s. Deep etch for SEM after 45 s." I hope this helps. You wrote: Question: What solution and method would one use to deep-etch Type 316 STAINLESS STEEL to reveal the inclusions and/or precipitates present in relief. This will be used for SEM analysis. Thankyou in advance for your assistance. Bruce
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
In a message dated 2/23/01 1:26:35 PM, pmiller-at-mbt.com-at-sparc5.microscopy.com writes:
} I would like to know if there are training courses anywhere in North } America on the statistics of quantitative microscopy or stereology. If } so, where, or where can I search? Pmiller-at-mbt.com
The N. C. State Univ. course on quantitative image processing and stereology has been taught for 20 years now. The next session is May 9-11 and there are still some openings available. You can get full information at http://members.aol.com/IPCourse including syllabus, lab materials and books, on-line registration, and a downloadable brochure, or call Cindy Allen at 919 515 8171
I am in the wonderful position of purchasing a new inverted metallograph with digital image acquisition for our laboratory.
I have pretty much decided on the Nikon Epiphot 200 metallograph with brightfield and polarized light modules. Also chosen were the Plan Achromat objectives. Any comments on this choice?
Now the real question. Which digital camera would you buy? I know these things have been discussed greatly here in the past and I have followed them with great interest.
One of our primary wants is live image preview(} 15 fps).
We are leaning towards the Spot RT, although the Insight looks to be equally promising.
Any comments for or against either of these would be appreciated. Also, if anyone has another preference I would appreciate hearing your comments.
Thanks
William T. Giles Sr. Electron Microscopist Met. Lab. Coordinator Henderson Technical Laboratory TIMET PO Box 2128 Henderson NV 89009 Ph: (702)566-4436 Fax: (702)564-9038 E-mail: Bill.Giles-at-timet.com
I know this isn't a microscopy question but suspect many of the microscopy core types on the listserver are responsible for the large, poster-sized format printers. I plan to buy one and would appreciate recommendations (both good and bad ones!). If you have any comments on the workload to maintain and use them, that would also be useful. TIA, tom
-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
Hi, I am wondering if anyone knows of a staining method that might allow one to distiguish between a region of proton implanted GaAs and an un-implanted region in a TEM?
Also, does anyone have a URL for a microscopy web forum?
-- David Mathes Department of Materials Science University of Virginia 116 Engineer's Way Charlottesville, VA 22904 804 982 5683
Just a few quick comments on the polymerisation of LR White:
I've been using microwave oven curing of LR White for over two years now, I and others who use this resin for gold labeling studies. The blocks section VERY easily, silver after silver section just roll off the ol' diamond knife edge.
Like others have reported, we also coat grids (300# nickel in these cases, sonicated before use to clean in 10% acetone, 10% HCl, 80% DW, plus two short rinses in acetone) with Formvar, then coat with evaporated carbon. THEN use them to pick up sections. They are very stable under the beam and we have expeienced virtually no stability problems.
Can't really say if microwave curing aids the stability of the sections or not. We do that primarily because it saves time, and the resulting blocks section easily.
We use the Formvar support film primarily to increase stability of sections because of the many gold labeling solutions and rinses the sections on grids have to go through, the carbon coating is primarily for stability under the TEM beam.
For deep etching of austenitic stainless steels use 10% bromine in methanol. see E.M. Mahla and N.A. Nielsen, Carbide Precipitation in Type 304 Stainless Steel-An Electron Microscope Study, Trans. ASM 43:290-322 (1951).
We just got an Epson 9500 large format printer (44 inch). Works great and was highly rated.
Best,
Angela
At 12:14 PM 02/23/2001 -0600, Tom Phillips wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Director, Core Imaging Facility American Museum of Natural History Central Park West at 79th Street New York, NY 10024-5192 USA
Has anyone used InSpeck calibration beads from Molecular Probes? I recently purchased InSpeck green and I tried several combinations of microscope and CCD camera settings but I can only read intensity values from 3 concentrations, i.e. if I adjust for the highest concentration I can only image (get readings)3 concentrations and the same is true if I start at the lowest concentration. Also the values I obtained for the 3 concentrations is not linear. Does anyone know if there are other beads available or how else can one calibrate the fluorescence intensity in a fluorescence microscope equipped with a 100W HBO lamp? I would appreciate comments or suggestions.
Thanks,
Cora Bucana Corazon D. Bucana, Ph.D. Dept. Cancer Biology UT M.D.Anderson Cancer Center 1515 Holcombe Blvd, Box 173 Houston, Texas 77030 Phone: 713-792-8106 FAX 713-792-8747
To observe inclusions it is better do not etch steel at all, because etching could hide them (especially deep etch). It is true for both polished cross sections and fracture surfaces. EDS and/or BSE detector could be helpful in the beginning when you are learning how to distinguish inclusions from other features of your sample.
Vladimir
} Question: What solution and method would one use to deep-etch Type 316 } STAINLESS STEEL to reveal the inclusions and/or precipitates } present in } relief. This will be used for SEM analysis. } Thankyou in advance for your assistance. } Bruce } } -------------------------------------------------------------- } ------------- } } }
Tom, I don't know your level of expertise but I'll give you a protocol that I've used for those antibodies on pancreatic cells grwon on coverslips. the protocol is pretty standard with LRW. I use 0.05M tris saline with 1%BSA + 0.05% Tween 20 for all solutions including washes. Place grids section side down on 30 ul drops. Filter all solutions. Note: I use different variations of this protocol but this one is easy to trouble shoot.
1. 15-20 min incubation in 5 to 10 %normal serum of animal used for the secondary antibody. 2. 1- 2 hrs in primary antibody. Try various dilutions, i.e, 1:50 to 1:500. This depends on a number of factors such as background, avidity of the antibody, etc. 1:50 is always a good one to include for one hour. It gives you a feel what changes you may need the next time. In cases where background is too high go overnight with higher dilutions in frig -at- 4C. 3. wash by placing grids on several drops of buffer, i.e. 6 drops 4. 30 -60min in 1:40, 5 or 10nm gold conjugated anti-IgG of the animal that the primary was produced in. 5. wash grids first with 5 drops of buffer followed by 5 drops of distilled water. 6. If you are going to dual label repeat the steps above but now your primary is from a different animal so your first step or blocking step is then different. Also you will used a different size gold particle conjugated secondary. 7. Counter stain with UA and lead. Contrary to what has been said I prefer staining very briefly in UA as a saturated soln in 50% ETOH followed by washing in a descending series of ETOH (50%,25% 10%) to water. I prefer the appearance of the chromatin stained with alcoholic UA. The only problems that I have is over staining if I go much beyond 5 to 10secs. In your case I would try it if you don't feel confident using alcoholic UA used 1% aqueous but stain longer like a minute The staining depends on your preferences. The only problem if you go to long you can't see the gold. Stain with 30 to 60 secs. I have been doing immunolabelling with LRW in everything from seeds to cells to mammary gland without too many problems. You have to be careful at all steps of the way but it works fine. I also stay away from filmed grids if I can and use 300 to 400 mesh nickel grids for many reasons especially when I know structures containing my epitope are common like what you are looking for and the descreased viewable area due to the grids bars is not a factor. LRW in our hands survives well the numerous staining steps. If you have any more questions email me. Good luck.
Hank Adams
Integrated Microscopy Core Molecular and Cellular Biology Baylor College of Medicine Houston, TX 77030
-----Original Message----- } From: Tom W Bargar [SMTP:tbargar-at-unmc.edu] Sent: Friday, February 23, 2001 6:17 PM To: Microscopy-at-sparc5.microscopy.com
Anyone have protocols for immunogold labelling of insulin and glucagon in pancreatic islet cells?
Also, many thanks to everyone who responded about the LR White stability problem.
Hallo All! I've noticed a bit of electronic noise starting to creep into our pulse processing electronics, and to eliminate it have turned the gain down a little accordingly. I have some ideas of where it may be coming from but thought I pick the brains of the wealth of experience out there. So, guys n' galls what gives rise to electronic noise in pulse processing electronics. Of course, I could just look up such elimentary stuff on some web page or other, but where's the fun in that? Malc.
-- Dr MP Roberts Phone: [+27](0)46 603 8313 Dept of Geology Fax: [+27](0)46 622 9715 Rhodes University Cell: 083 4060 262 (usually off) 6140 Grahamstown e-mail: m.roberts-at-ru.ac.za SOUTH AFRICA
Available with the Center for High Resolution Electron Microscopy at Arizona State University. Position is sponsored by major chemical company and primary focus of the research will be the development and application of environmental electron microscopy to industrially relevant catalyst systems. Areas of particular interest include the study of phase transformations under reaction environments, in situ polymerization, mobility and dynamic microstructural changes. Candidate will have a Ph.D. in material science, material physics, solid-state chemistry or chemical engineering, with extensive experience in catalyst characterization by transmission electron microscopy. Experience in the areas of catalyst synthesis, testing and characterization is preferred. Please submit your resume together and the names of 3 referees to: Dr. Peter A. Crozier, Industrial Associates Program, Center for Solid State Science, Arizona State University, Tempe, AZ 85287-1704, Fax (480) 965-9004, email: crozier-at-asu.edu.
John C. Wheatley Lab Manager Arizona State University Center for Solid State Science PSA-213 BOX 871704 Tempe, AZ 85287-1704
This thread and a conversation with a colleague here in Dallas recently has gotten me wondering. Has anyone else come across a situation where a formulation for a polymer works fine in one area of the country, but you can't get it to work in another? For instance, years ago when I was working in Minnesota, we used a soft formula for Spurs with fairly good results yet here in Dallas, Tx., I have to use the hardest formula to get a block that is hard enough to section and still some- times the blocks are soft. I also remember that we always had a whole different set of problems to deal with in Minnesota depending on whether it was in the middle of the very dry winter or in the humid spring and summer.
My colleague here in Dallas is trying to use a two part polymer for casting. He has used it successfully some years ago in another part of the country and now he is getting too soft results. I don't know about his protocol, but I have tried adding drierite to the chamber that my blocks are polymerized in, but it has met with limited success.
I also remember a story my old boss told me about his bringing some already cured "epon" blocks that had already been successfully sectioned in Minnesota with him on sabbatical. The blocks were like goo when he tried to section them the second time in Sweden. (This was in the late '60s, early 70's.)
Could the success that you see, Gib, and the lack of success the others are having be related to your location and the "elements"? I have never used a microwave for curing, so maybe that's the difference. Does that shorten the cure time significantly?
Anybody care to comment?
Karen Pawlowski
Gib Ahlstrand wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi Microlisters, } } Just a few quick comments on the polymerisation of LR White: } } I've been using microwave oven curing of LR White for over two years now, I } and others who use this resin for gold labeling studies. The blocks section } VERY easily, silver after silver section just roll off the ol' diamond knife } edge. } } Like others have reported, we also coat grids (300# nickel in these cases, } sonicated before use to clean in 10% acetone, 10% HCl, 80% DW, plus two } short rinses in acetone) with Formvar, then coat with evaporated carbon. } THEN use them to pick up sections. They are very stable under the beam and } we have expeienced virtually no stability problems. } } Can't really say if microwave curing aids the stability of the sections or } not. We do that primarily because it saves time, and the resulting blocks } section easily. } } We use the Formvar support film primarily to increase stability of sections } because of the many gold labeling solutions and rinses the sections on grids } have to go through, the carbon coating is primarily for stability under the } TEM beam. } } Gib Ahlstrand
I was analyzing some foam samples and observed small disks (about 25 microns) on the cell walls on one sample. Does anyone out there know what they are? The foam is non-ridged.
I'm Okugawa from Nikon Japan. The adapter is available in China. Could you contact the following woman who is representative in Beijing office.
Product: MXA29005 Contact: Ms. Wang, phone: 010-6515-5637 or 5639
Hisashi Okugawa 1st design department Instrument company in Nikon Corporation Phone: 81-45-853-8568 Fax: 81-45-853-8475 e-mail: okugawa.h-at-nikon.co.jp ----- Original Message ----- } From: "cenpok" {dyy-at-cenpok.net} To: {microscopy-at-sparc5.microscopy.com} Sent: Saturday, February 24, 2001 11:38 AM
Dear Dr.Dong,
This is Okugawa again.
What application do you like to use NIR microscope for? How long wavelength are you interested? Objective effects the image performance. Most of our standard objectives transmit and make good images until 1100nm. Many of them transmits more than 50% and some transmit more than 70%. I may help you technically with your requests. Please contact the e-mail below personally.
Hisashi Okugawa 1st design department Instrument company in Nikon Corporation Phone: 81-45-853-8568 Fax: 81-45-853-8475 e-mail: okugawa.h-at-nikon.co.jp ----- Original Message ----- } From: "cenpok" {dyy-at-cenpok.net} To: {microscopy-at-sparc5.microscopy.com} Sent: Saturday, February 24, 2001 1:17 PM
How would you like to take all of your credit cards, reduce or eliminate the interest, pay 70% less per month, and pay them off 70% sooner?
In addition to the polarized light module, I would suggest getting the DIC (Nomarski) module. This will provide contrast to low contrast etched microstructures such as martensite and acicular structures. We find it very useful for low carbon steels.
Sam Purdy Technical Center National Steel Corp. Trenton, MI spurdy-at-nationalsteel.com
The Department of Materials, University of Oxford, UK, has three vacancies in its Electron Microscope Facility - one senior Engineer and two technicians. The details can be found at http://www.materials.ox.ac.uk/vacancies/vacancieshome.html#SupportStaff.
Closing date for applications is 23rd March 2001.
Please bring this to the attention of anyone who may be interested.
Thanks Ron
---------------------- Mr. R.C. Doole Department of Materials, University of Oxford. Parks Road, Oxford. OX1 3PH. UK. Phone +44 (0) 1865 273701 Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
Dear Gib and All, We get calls from all over the country and the increase in technical calls with problems is different for some areas. The time of year and weather conditions can and do play a part in how somethings react and polymerize. It is difficult to tell when or where this will happen and often we recommend drying with dissicant at one point and adding moisture for others. This sounds a little strange and as if the question is being avoided however, you bring up a good point and the changes in the environment should be considered when getting a protocol from a colleague in another area or country. It has caused people to become very angry when they think something is left out or not discussed. in actuality the problem can be the change in environment or even pH of the distilled, DI, or tap water. Histology has these problems often with season changes and the addition of heat or cooling of the laboratory air. Pam Marcum
-----Original Message----- } From: Karen Pawlowski [mailto:Karen.Pawlowski-at-worldnet.att.net] Sent: Saturday, February 24, 2001 1:18 PM To: Gib Ahlstrand Cc: Microscopy-at-sparc5.microscopy.com
Hi Gib and all,
This thread and a conversation with a colleague here in Dallas recently has gotten me wondering. Has anyone else come across a situation where a formulation for a polymer works fine in one area of the country, but you can't get it to work in another? For instance, years ago when I was working in Minnesota, we used a soft formula for Spurs with fairly good results yet here in Dallas, Tx., I have to use the hardest formula to get a block that is hard enough to section and still some- times the blocks are soft. I also remember that we always had a whole different set of problems to deal with in Minnesota depending on whether it was in the middle of the very dry winter or in the humid spring and summer.
My colleague here in Dallas is trying to use a two part polymer for casting. He has used it successfully some years ago in another part of the country and now he is getting too soft results. I don't know about his protocol, but I have tried adding drierite to the chamber that my blocks are polymerized in, but it has met with limited success.
I also remember a story my old boss told me about his bringing some already cured "epon" blocks that had already been successfully sectioned in Minnesota with him on sabbatical. The blocks were like goo when he tried to section them the second time in Sweden. (This was in the late '60s, early 70's.)
Could the success that you see, Gib, and the lack of success the others are having be related to your location and the "elements"? I have never used a microwave for curing, so maybe that's the difference. Does that shorten the cure time significantly?
Anybody care to comment?
Karen Pawlowski
Gib Ahlstrand wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi Microlisters, } } Just a few quick comments on the polymerisation of LR White: } } I've been using microwave oven curing of LR White for over two years now, I } and others who use this resin for gold labeling studies. The blocks section } VERY easily, silver after silver section just roll off the ol' diamond knife } edge. } } Like others have reported, we also coat grids (300# nickel in these cases, } sonicated before use to clean in 10% acetone, 10% HCl, 80% DW, plus two } short rinses in acetone) with Formvar, then coat with evaporated carbon. } THEN use them to pick up sections. They are very stable under the beam and } we have expeienced virtually no stability problems. } } Can't really say if microwave curing aids the stability of the sections or } not. We do that primarily because it saves time, and the resulting blocks } section easily. } } We use the Formvar support film primarily to increase stability of sections } because of the many gold labeling solutions and rinses the sections on grids } have to go through, the carbon coating is primarily for stability under the } TEM beam. } } Gib Ahlstrand
I read the literature available on the indicated web site and have a couple of questions.
There seems to be some conflicting information. Under specifications: Image size 3840 x 3072 CCD 1.34 M pixels Document text: 12 million pixels (3046 x 3072) ???????
Is my math wrong or am I missing some pertinent information?
How do you obtain RGB? What does the color array on the chip look like? Or does the camera use filters?
What's the s/n ratio?
Pixel size?
Are there twain drivers available or a Photoshop plugin?
Is any annotaion, image enhancement and/or image analysis software included?
BTW, the Spot RT is $7k also, not twice that price.
William T. Giles Sr. Electron Microscopist Met. Lab. Coordinator Henderson Technical Laboratory TIMET PO Box 2128 Henderson NV 89009 Ph: (702)566-4436 Fax: (702)564-9038 E-mail: Bill.Giles-at-timet.com
} -----Original Message----- } From: Lawrence Kordon [SMTP:nikon-at-jagunet.com] } Sent: Friday, February 23, 2001 1:38 PM } To: Giles, Bill } Cc: 'Microscopy-at-MSA.Microscopy.Com' } Subject: Re: Metallograph and Digital Camera for Light Microscopy } } William, } } Excellent choice on the Nikon Epiphot 200! However, why are you not } considering } the Nikon DXM1200 Digital Camera? It is a whopping 12 Million pixels with } a live } Video Output to a PC and cost under $7000. That is about the half the } price of } the Spot-RT. Also, why would you want to have a cooled CCD anyway? } Metallurgy; } even with Fluorescence and high Mag DIC (Pol) does not require it. Not to } mention this camera can do extremely low light anyway! This Camera is } literally } the hottest product in Microscopy today! We have sold hundreds already } "sight/unseen" and are first shipping them. All the major Image Analysis } companies have or are writing Drivers and it is so popular we have put it } on } other brands microscopes as well! By the way, the US MINT and some NIST } Labs } here is Washington, DC have it. Please check out the specs at.... } http://www.nikonusa.com/usa_product/product.jsp?cat=5&grp=26&productNr=DXM } 1200 } and call you local Nikon Dealer for a Demonstration and Quotation. } } Good Luck, } } Lawrence Kordon } Nikon Instruments, Inc. } Columbia, MD } nikon-at-jagunet.com } } } } "Giles, Bill" wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } Good Day Listers, } } } } I am in the wonderful position of purchasing a new inverted metallograph } } with digital image acquisition for our laboratory. } } } } I have pretty much decided on the Nikon Epiphot 200 metallograph with } } brightfield and polarized light modules. Also chosen were the Plan } Achromat } } objectives. Any comments on this choice? } } } } Now the real question. Which digital camera would you buy? I know these } } things have been discussed greatly here in the past and I have followed } them } } with great interest. } } } } One of our primary wants is live image preview(} 15 fps). } } } } We are leaning towards the Spot RT, although the Insight looks to be } equally } } promising. } } } } Any comments for or against either of these would be appreciated. Also, } if } } anyone has another preference I would appreciate hearing your comments. } } } } Thanks } } } } William T. Giles } } Sr. Electron Microscopist } } Met. Lab. Coordinator } } Henderson Technical Laboratory } } TIMET } } PO Box 2128 Henderson NV 89009 } } Ph: (702)566-4436 } } Fax: (702)564-9038 } } E-mail: Bill.Giles-at-timet.com
I've been asked to distribute the following announcement of an available position for a TEM technician at the University of Utah. Anyone who's interested should contact Dr. Kendal Broadie.
Ed King ____________________________
Electron Microscopy Lab Technician
The ideal candidate will have a bachelor degree in a biological science and experience in electron microscopy. Exposusure to neuroscience and/or genetics would be a benefit. The job includes preparing specimens for microscopy, operation of electron microscopes, producing photographic and digital micrographs and analyzing ultrastructural data. Training will be provided. If interested, please submit an application at the University of Utah Human Resources. If you would like more information about job specifics, please contact me (Kendal Broadie) at e-mail: broadie-at-biology.utah.edu
Dr. Kendal S. Broadie (801) 585-9426 (office; 448 Skaggs) Department of Biology (801) 585-9425 (main lab; 450 Skaggs) University of Utah (801) 585-1301 (lab II; 460 Skaggs) 257 South 1400 East (801) 581-4668 (fax) Salt Lake City, Utah 84112-0840 broadie-at-biology.utah.edu
I am looking for micro-crystalline quartz crystals, up to 100 microns in size. Does anybody know of a source? Thank you, Peggy. --
Dear Peggy, I have seen some small quarz crystals while examining river sediment. They're about 1 micron in size, EDS shows lots of Si, and no Na, Al, K, Ca, Fe or Ti--the most common other elements in silicates. I'm also not sure how best to separate them from the other material, although I would think that floating the sediment on an appropriate liquid would work. The Handbook of Chemistry and Physics has a table of Heavy Liquids for Mineral Separation on page E-376 (62nd Edition). Good luck. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
Two newly funded positions are available to study intracellular protein trafficking using LM and EM immunocytochemistry. Ideally, these positions will be filled by either two postdoctoral fellows or one postdoc and one senior research technician. The projects use similar approaches but are funded by separate sources.
The first project involves using confocal microscopy and electron microscopy, to visualize lipoproteins, lipid synthesizing enzymes, and components of the ubiquitin-proteasome system in hepatic and intestinal cells. Wild-type and heterologous cells will be used to map the location of these proteins in cell compartments and the secretory pathway. Live cell recording using transgenic cells with GFP-labeled constructs are part of this study.
The second project examines the intracellular trafficking and storage of transgenic proteins in maize and other plant seed tissues. The ideal candidate would have experience in plant microscopy and/or light and electron microscopic immunocytochemistry.
Individuals with experience in electron microscopy and prior training in ultramicrotomy are especially encouraged to apply for either position.
Please send a curriculum vitae, cover letter and the names of three references to Dr. Tom Phillips, Division of Biological Sciences, 3 Tucker Hall, University of Missouri, Columbia, MO 65211 7400. Enquires can also be directed to PhillipsT-at-missouri.edu.
-- Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
For your Nikon adapter I would suggest you contact McCrone Microscopes & Accessories from Westmont, IL 60559. They can be contacted on the web at www.mccrone.com or e-mail address info-at-mccrone.com or phone 630-887-7100 or Fax 630-887-7764. If they don't have it they should be able to direct you to who can.
As far as the 1992 Microscopy Analysis of Feedstuffs you can contact the American Oilseed Chemist Society at www.aocs.org. Please contact them and they will be happy to help.
George Falb AOCS/Feed Microscopy Division Chair
----- Original Message ----- } From: "cenpok" {dyy-at-cenpok.net} To: {microscopy-at-sparc5.microscopy.com} Sent: Friday, February 23, 2001 9:38 PM
dear all, does anyone know where the microscope adapter for nikon coolpix 990 could be available?
does anyone know where can I get mannual of microscopial analysis of feedstuffs, 1992 3rd edition, the americian association of feed microscopists?
I'm doing my master in materials science and recently a BSE detector was
installed in the SEM. I'm working with it and I want to know how the detector physically works. The manual just said that the detector is made of semiconducor material. The detector is a GW Electronics system 47 solid state detector. Could some body help me with that description ??
Xinran, I have not had too much experience in vibratoming mouse brains, but I do vibratome rat brains on a regular basis. I have only had problems when the tissue has not been fixed long enough, or when I am cutting 25-30 um sections. Usually turning down the amplitude to a very low setting has worked. Sorry that I couldn't be much more help. Contact me if you have any other specific questions. KElly Randall Henry Ford Hospital kellyr-at-neuro.hfh.edu
On Fri, 23 Feb 2001, Xinran Liu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, colleagues, } } We have a question regarding a trouble shooting of chatter/compression on } brain sections. } } The vibratome we used is VIBRATOME 3000, automated with refrigeration, the } mouse brain tissue was fixed in 4% paraformaldehyde, the thickness of } section cut was 30-50 um. We have being adjusting either speed or amplitude, } however, it doesn't seem to solve the problem. } } We would appreciate anyone who would kindly help us to solve this problem. } } Many thinks. } } Xinran } } *********************************************************** } Xinran Liu, M.D., Ph.D. } Center for Basic Neuroscience } UT Southwestern Medical Center } 6000 Harry Hines Blvd., NA4. 214A } Dallas, TX 75390-9111 } } Phone: (214) 648-1830 } Fax: (214) 648-1801 } E-mail: Xinran.Liu-at-UTsouthwestern.edu } } }
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Pathology lab. listers: are there any new/and/or superior techniques for processing previously formalin fixed tissue for TEM? Thanks, Peggy Harger-Allen |TAB|email:harger-allen-at-indianapolis.va.gov
by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id PAA03591 for dist-Microscopy; Mon, 26 Feb 2001 15:55:23 -0600 (CST) Received: from no_more_spam.com (sparc5 [206.69.208.10]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with SMTP id PAA03588 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Mon, 26 Feb 2001 15:54:53 -0600 (CST) Received: from smtp.rcsis.com (smtp.rcsis.com [208.45.228.18]) by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id PAA03581 for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 26 Feb 2001 15:54:41 -0600 (CST) Received: from ggaugler.gaugler.com (241.dsl108148.rcsis.com [63.108.148.241]) by smtp.rcsis.com (8.9.3/8.9.3) with ESMTP id NAA13323; Mon, 26 Feb 2001 13:49:30 -0800 Message-Id: {5.0.2.1.2.20010226132955.00a8f900-at-pop.calweb.com} X-Sender: gaugler-at-pop.calweb.com (Unverified) X-Mailer: QUALCOMM Windows Eudora Version 5.0.2
The Nikon MX1200 is native 1.2M pixels. It, like current generation digital microscope cameras, do a "mechanical" interpolation to increase final pixel count. One way is to physically move the CCD imager. Each movement results in new image data for that position. This is a bad way to gather more pixels, however. The other way is to keep the CCD fixed but scan the image across it multiple times at different angles.
Zeiss has tried to do mechanical shifting and not done all that good of a job. Looks like Nikon is trying it too. They are up against a basic patent by Pixera. Pixera uses what they call a "Diractor" to scan the image across a fixed CCD.
The Pixera is available as a 1.5M pixel unit or as a 5.8M pixel Diractor unit. Each are also available with Peltier cooling. Like the Nikon, the Pixera uses a dedicated PCI card for PC or Mac. This card, camera and software will do live focus at 12-15 fps on a 733MHz system. It does manual and automatic exposure and also provides a focus indication bar. Also provided is automatic or manual white balance, selectable black balance and multi-size spot exposure metering.
The best way to find out how well a camera performs is to take a high resolution shot at different lighting extremes and do an FFT on the images. Artifacts and garbage will show up if the system produces high pixel count at the expense of image quality.
I use a Pixera Penguin 600CL and like it very much. It is easy to take single shots either as one exposure or averaged or integrated up to 256 exposures. Averaging and Peltier cooling greatly reduces ultimate noise in the image. For simple documentation purposes, this is not necessary. But for images which will undergo further processing, noise and artifacts must be reduced or eliminated. The Pixera has selectable ISO from 50 to 400. I run it at ISO 100. No info on what the Nikon does.
The Pixera also supports Twain interfacing.
gary g.
At 08:57 AM 2/26/01, you wrote:
} Lawrence, } } Thanks for your response. } } I read the literature available on the indicated web site and have a couple } of questions. } } There seems to be some conflicting information. } Under specifications: } Image size 3840 x 3072 } CCD 1.34 M pixels } Document text: 12 million pixels (3046 x 3072) ??????? } } Is my math wrong or am I missing some pertinent information? } } How do you obtain RGB? What does the color array on the chip look like? Or } does the camera use filters? } } What's the s/n ratio? } } Pixel size? } } Are there twain drivers available or a Photoshop plugin? } } Is any annotaion, image enhancement and/or image analysis software included? } } BTW, the Spot RT is $7k also, not twice that price. } } William T. Giles } Sr. Electron Microscopist } Met. Lab. Coordinator } Henderson Technical Laboratory } TIMET } PO Box 2128 Henderson NV 89009 } Ph: (702)566-4436 } Fax: (702)564-9038 } E-mail: Bill.Giles-at-timet.com } } } -----Original Message----- } } From: Lawrence Kordon [SMTP:nikon-at-jagunet.com] } } Sent: Friday, February 23, 2001 1:38 PM } } To: Giles, Bill } } Cc: 'Microscopy-at-MSA.Microscopy.Com' } } Subject: Re: Metallograph and Digital Camera for Light Microscopy } } } } William, } } } } Excellent choice on the Nikon Epiphot 200! However, why are you not } } considering } } the Nikon DXM1200 Digital Camera? It is a whopping 12 Million pixels with } } a live } } Video Output to a PC and cost under $7000. That is about the half the } } price of } } the Spot-RT. Also, why would you want to have a cooled CCD anyway? } } Metallurgy; } } even with Fluorescence and high Mag DIC (Pol) does not require it. Not to } } mention this camera can do extremely low light anyway! This Camera is } } literally } } the hottest product in Microscopy today! We have sold hundreds already } } "sight/unseen" and are first shipping them. All the major Image Analysis } } companies have or are writing Drivers and it is so popular we have put it } } on } } other brands microscopes as well! By the way, the US MINT and some NIST } } Labs } } here is Washington, DC have it. Please check out the specs at.... } } http://www.nikonusa.com/usa_product/product.jsp?cat=5&grp=26&productNr=DXM } } 1200 } } and call you local Nikon Dealer for a Demonstration and Quotation. } } } } Good Luck, } } } } Lawrence Kordon } } Nikon Instruments, Inc. } } Columbia, MD } } nikon-at-jagunet.com } } } } } } } } "Giles, Bill" wrote: } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } Good Day Listers, } } } } } } I am in the wonderful position of purchasing a new inverted metallograph } } } with digital image acquisition for our laboratory. } } } } } } I have pretty much decided on the Nikon Epiphot 200 metallograph with } } } brightfield and polarized light modules. Also chosen were the Plan } } Achromat } } } objectives. Any comments on this choice? } } } } } } Now the real question. Which digital camera would you buy? I know these } } } things have been discussed greatly here in the past and I have followed } } them } } } with great interest. } } } } } } One of our primary wants is live image preview(} 15 fps). } } } } } } We are leaning towards the Spot RT, although the Insight looks to be } } equally } } } promising. } } } } } } Any comments for or against either of these would be appreciated. Also, } } if } } } anyone has another preference I would appreciate hearing your comments. } } } } } } Thanks } } } } } } William T. Giles } } } Sr. Electron Microscopist } } } Met. Lab. Coordinator } } } Henderson Technical Laboratory } } } TIMET } } } PO Box 2128 Henderson NV 89009 } } } Ph: (702)566-4436 } } } Fax: (702)564-9038 } } } E-mail: Bill.Giles-at-timet.com
I am going off my recollection, which can sometimes be a dangerous thing, but it should be a start and may be adequate.
I believe your GW BSE detector is very similar in principle to solar voltaic cells. Incident photons create electron-hole pairs which generate a current/voltage. You will find that such detectors are swamped when the SEM chamber light is turned on or the chamber is opened to room light.
In the other camp are Robinson style BSE detectors. These are better considered as scintillator type detectors. Incident electrons generate a pulse of light which is directed down a light pipe to a photo-multiplier tube. This kind can generate a signal suitable for TV-rate imaging, but may not be very sensitive to low signal levels. The former kind may be more sensitive, but are slower responding and suitable primarily for slow scans.
The most basic of SEM books should describe the difference between backscattered and secondary electrons. The intensity of the BSE signal is directly related to the atomic number of the phase under the beam. Therefore, the BSE signal gives indications of compositional variation even without EDX. I also remember seeing some articles that talked of doing spectroscopy on the BSE signal (i.e., measuring energy of the scattered electrons) and relating that to the material doing the scattering. However, basic BSE systems are not setup for such measurements.
Hope this gets you started.
At 11:05 AM 2/26/2001 -0600, you wrote:
} Hello all, } } I'm doing my master in materials science and recently a BSE detector was } } installed in the SEM. I'm working with it and I want to know how the } detector } physically works. The manual just said that the detector is made of } semiconducor material. The detector is a GW Electronics system 47 solid } state detector. Could some body help me with that description ?? } } } Laura Lopez } CCMC-UNAM } Ensenada, B.C. Mexico
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
Laura, The BSE detector is basically a solar cell and is able to generate a current from the energy released by backscattered eleectrons impinging on its surface. It is fairly fragile, so don't run a specimen up into it. Also GW has done a lot of work on this and a regular solar cell won't work because it has too much capacitance. These are very thin. I also seem to remember that they went to a cell that was doped opposite to the doping normally found in solar cells (P instead of N or vice versa). The signal is then run through a preamplifier and on to the main amplifier. For TV rates (if that was set up) I believe they reverse bias the cell to further reduce capacitance and increase gain (sensitivity). Secondary electrons don't have nearly enough energy to cause the cell to react.
Ken Converse owner Quality Images Delta, PA
Laura Lopez wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } } Hello all, } } I'm doing my master in materials science and recently a BSE detector was } } installed in the SEM. I'm working with it and I want to know how the } detector } physically works. The manual just said that the detector is made of } semiconducor material. The detector is a GW Electronics system 47 solid } state detector. Could some body help me with that description ?? } } } Laura Lopez } CCMC-UNAM } Ensenada, B.C. Mexico } } } }
There VERY OLD, however they were manufactured by the Cambridge Instrument Company, now part of LEO. (Cambribge SEMs). They may be able to help you. At one stage the Huxley Mk2 were distributed by LKB, now part of the Leica Instruments Company, they also may be able to help you. Regards JVN
Steve Widing wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hello List, } } Does anyone have a contact or supplier for parts for a Huxley } Ultra Microtome MK2? } } Thanks, } } Steve Widing } Temple University
-- John Nailon Operations Manager The Centre for Microscopy and Microanlaysis The University of Queensland St Lucia QLD 4072 Tel: +61-7-33654214 Fax: +61-7-33654422 WWW: http://www.uq.edu.au/nanoworld
-----Original Message----- } From: Ronald Austin [mailto:rla-at-mindspring.com] Sent: Monday, February 26, 2001 4:39 PM To: "HARGER-ALLEN.MARGARET_J-at-INDIANAPOLIS.VA.GOV"-at-sparc5.microscopy.com
Pathology lab. listers: are there any new/and/or superior techniques for processing previously formalin fixed tissue for TEM? Thanks, Peggy Harger-Allen |TAB|email:harger-allen-at-indianapolis.va.gov
I was wondering if anyone might have an extra filter cube holder laying around for an older Nikon Diaphot inverted microscope.
Any help would be greatly appreciated.
Thanks,
Raj
********************************************** Dr. Raj Lartius, CEO NovaScan Technologies Iowa State University Research Park 2501 North Loop Drive Ames, Iowa USA 50010
Email: rlartius-at-novascan.com Voice: 515-795-3164 Fax: 515-795-4414 ********************************************** "Innovative Tools to Explore the Microworld"
Email: risports-at-aol.com Name: Keenan Kelly School: Veterans Memorial HS
Question: I recently bought an American Optical Model 150 microscope used on eBay and would like to add phase contrast to it.
Question 1.) Why can't I find a web site or other company information about American Optical on the web?
Question 2.) Are objectives and condensers interchangeable between microscopes from different manufacturers? If not all, then how do you find out which are?
Email: maegan-at-ualr.edu Name: Marilyn Egan School: University of Arkansas at Little Rock
Question: Are you familure with dispersion staining techniques? I need information regarding microscope set up and grain orientation with respect to the annular and central stops.
Fix in glutaraldehyde, dehydrate through a gradient ethanol and acetone series, critical point dry, glue to a stub and sputter-coat or --attach to a specimen mount for cryo-sem, freeze, etch by raising the temperature to -100C, sputter-coat and image.
I am looking for a nice glue to fix the specimen to the tripod polisher for wedge polishing. The "super glue" stuff does not work well. The specimen falls off too easily.
I would appreciate very much if anyone could send me some good idea.
This week, our CPD was broken. I was planning to CPD some samples that day. The samples are now in acetone. My question : how long can these samples stay in acetone ? The samples are epithelial cells from duodenum grown on glass.
Nikon DXM1200 has 1.3M pixels CCD. With 3 x 3 times "Diractor", it makes 12M pixels images. Because of the color array, 2 pixels should be moved totally. DXM1200 moves two times every two thirds pixels. Pixera moves 1 time every one pixel or three times every half pixels. Please visit the following web to get more information
http://www.microscopyu.com/
Hisashi Okugawa 1st design department Instrument company in Nikon Corporation Phone: 81-45-853-8568 Fax: 81-45-853-8475 e-mail: okugawa.h-at-nikon.co.jp ----- Original Message ----- } From: "Gary Gaugler" {gary-at-gaugler.com} To: "Giles, Bill" {William.Giles-at-TIMET.com} Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com} Sent: Tuesday, February 27, 2001 6:52 AM
Dear Sam,
DXM1200 is high sensitive enough to get good DIC images or some fluorescence images.
Hisashi Okugawa 1st design department Instrument company in Nikon Corporation Phone: 81-45-853-8568 Fax: 81-45-853-8475 e-mail: okugawa.h-at-nikon.co.jp ----- Original Message ----- } From: "Purdy, Sam" {SPurdy-at-nationalsteel.com} To: {Microscopy-at-sparc5.microscopy.com} Sent: Monday, February 26, 2001 11:28 PM
If those cells were for TEM sections, I would be very concerned and re-hydrate them back to 70% alcohol immediately. For SEM lipid extractions matters less but you may well get some shrinkage. Its less urgent, but I would take those cells back to 70%. At that and refrigerated you SHOULD see no real change for some weeks. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Tuesday, February 27, 2001 7:56 PM, Bart De Pauw [SMTP:Bart.DePauw-at-rug.ac.be] wrote: } } Hello Everyone, } } This week, our CPD was broken. I was planning to CPD some samples that } day. The samples are now in acetone. My question : how long can these } samples stay in acetone ? The samples are epithelial cells from duodenum } grown on glass. } } greetings, } } De Pauw Bart } Belgium }
I have been given mouse eyes in 2% paraformaldehyde/2.5% glutaraldehyde buffered with Millonig's phosphate buffer. I was asked to rate the lens for opacity and then to fix the eyes in tact and section them. Any suggestions would be appreciated. Thanks.
{html} {DIV} Hi all, {/DIV} {DIV} I'm from the university of Massachusetts, Lowell. We are looking for a microtome to replace the old one we have. does anyone have a old microtome they want to sell off or just trying to get rid of? {/DIV} {DIV} Praveena {/DIV} {DIV} Department of Chemical Engineering {/DIV} {DIV} Unversity of Massachusetts, Lowell {/DIV} {DIV} Lowell , MA {/DIV} {DIV} PH: 978-934-3411 {/DIV} {DIV} {/DIV} {br clear=all} {hr} Get Your Private, Free E-mail from MSN Hotmail at {a href="http://www.hotmail.com"} http://www.hotmail.com {/a} . {br} {/p} {/html}
Dear rad0, I have looked at fungus and fungus spores on my SEM and they are very simple to prepare. Just stick them to a stub by sticky tab or glue and then gold coat. They are very robust and they don't need any fixing or dehydration. At 06:06 PM 2/26/01 -0600, you wrote: } Hello, } } Forgive me, I haven't tried to find this on my own yet... } } But have any of you used an electron microscope to get a } close-up of a fungus? Can this be done? } } Thanks... } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
You might try exposing your non-aquatic sample (bread molds, mushrooms, etc. ) to vapors of Osmium tetroxide for several hours ( a few drops of 4% OsO4 on the lid of a very small container with your sample inside or the lid over the sample directly), then air dry, mount, coat and view. As with any sample, the drying artifacts vary with the fungus. Some things look great.
good luck
Steve } } Hello, } } Forgive me, I haven't tried to find this on my own yet... } } But have any of you used an electron microscope to get a } close-up of a fungus? Can this be done? } } Thanks...
___________________________________________________ Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619) 594-4523 fax: (619) 594-5676
Chairman, Educational Outreach subcommittee promoting microscopy instruction and increased access to microscopes Microscopy Society of America http://www.msa.microscopy.com/
There has been a lot of discussion over the past on this subject. The boys from IBM are the experts. (There's your plug, Ron.) Since nobody has answered this yet, I'll give you my summary. Ron, If I say anything wrong, please correct me.
Don't use any type of super glue gel! It doesn't work, you have to use the thin stuff.
The IBM folks (Ron et al.) recommend Ross super glue, but that is sometimes difficult to locate in other parts of the country.
I have had good success with the Alpha superglue from Electron Microscopy Sciences and with Loctite superglue available almost anywhere. Buy it from a place that has a high turnover rate of it so that it is fresh. The IBM guys recommend keeping the stuff unopened in the refrigerator for any length of time. Once it has been opened, you can't use it for very long (a day or two). I have used the Loctite a little longer because it has a very good sealing top.
I think that pressure on the sample while it cures is important, but the IBM guys just wick the extra stuff away from the sample and don't put a lot of pressure on the sample.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
-----Original Message----- From: Csaba Cserhati [mailto:cserhati-at-delfin.klte.hu] Sent: Tuesday, February 27, 2001 4:22 AM To: Microscopy-at-sparc5.microscopy.com Subject: Tripod Polisher
--------------------------------------------------------------- --------- The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
I am looking for a nice glue to fix the specimen to the tripod polisher for wedge polishing. The "super glue" stuff does not work well. The specimen falls off too easily.
I would appreciate very much if anyone could send me some good idea.
At 9:37 AM -0500 2/27/01, stacey andringa wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
*********************** Hi Stacey,
If you must leave the eyes intact, I would suggest REALLY prolonging everything: at least 30 minutes in each alcohol, and take about a day & a half for the infiltration. Use a rotator during all steps. I've had better luck using Epon-Araldite for eyes, although I've also used Spurr's.
If its at all possible, find out if you (or the person you are doing this for) can make a few small holes through the sclera to give better access of solutions to the internal structures. eyes are very complex, with lots of layers of different cell types and different consistencies, so you really need to get your reagents to penetrate thoroughly. The vitreous, in the center, is frequently problematic and one of the main reasons for needing the long steps.
Eyes are not easy, but when you've don a good job, they are beautiful. Stain your 1 micron sections with methylene blue...gorgeous!
Good luck, Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
What are some good demonstration specimens that clearly show phase halo and shading off? I am especially interested in demonstrating shading off.
Bob
_____________________________________________________________ C. Robert Bagnell Jr., Ph.D., Res.Assoc.Prof. Microscopy Services Laboratory Department of Pathology & Laboratory Medicine CB #7525 UNC-CH, Chapel Hill, N.C. 27599 ph 919-966-2413 fx 919-966-6718 http://www.pathology.med.unc.edu/path/microscopy/welcome.htm
CONFOCAL MICROSCOPIST POSITION OPEN AT GENZYME CORPORATION (FRAMINGHAM, MA)
Position: Scientist- Staff I
Description of Duties:
This individual will be in charge of Genzyme's confocal microscopy facility, and will interact with multiple research groups across the Company who utilize this technology. The individual will also be a key player in research efforts within the Pathology department, and will be a leader in fluorescent microscopy, image acquisition and image analysis of cell culture and tissue models of disease.
Experience/Skills/Education required:
Expertise in confocal microscopy and fluorescent microscopy required. The individual must have a good working knowledge of molecular biology, cell biology and cell culture, and histology. Strong computer skills are a must, with expertise in digital image acquisition and image analysis using Metamorph, Image Pro Plus and Photoshop. Highly motivated self-starter, with effective supervising skills. Excellent interpersonal skills, written and oral presentation skills and team approach to research are a necessity, as this individual will be interfacing with multiple teams throughout the company. Ph.D. in cell and molecular biology, with 0-3 years post-doctoral experience. Prior industrial experience a plus.
Please send resumes by US mail ONLY. Please do not reply to this message. Resumes sent by email will NOT be considered.
______________________________ B. Thurberg, M.D., Ph.D. Associate Director of Pathology Preclinical Biology Genzyme Corporation One Mountain Road P.O. Box 9322 Framingham, MA 01701-9322
I have looked extensively at a wild strain of Aspergillus many years ago. I grew the fungus on sterilized glass slides with half-strength saubourod-dextrose agar for at t least two days and up to 21 days. I cut out pieces of the agar culture and fixed in 1% osmium fumes fix overnight under cover, then a standard dehydration in ETOH, two changes of amyl acetate and then critical point drying. These were sputter coated several times as these were difficult to view without charging. I never published this, but I dragged the paper out recently after 22 or so years to see if I could do SEM on Candida. Funny you should also ask! Good luck and if you have any other question, you can reach me at Bplowman-at-sfuop.edu.
Barbara L. Plowman Univ. of the Pacific School of Dentistry 2155 Webster Rm 632 San Francisco, CA 94115 ph: 415-929-6692 FAX: 415-929-6654 email: Bplowman-at-sfuop.edu
Can anyone tell me if the required currents for the different brands of LaB6 filaments are significantly different?
The reason that I am asking this is that it appears that on my JEOL 2000FX that I can not fully saturate the LaB6 filament when it is set up correctly, i.e. tip to whenelt distance, centering, biasing. There appears to be a limit at which no more current is available as you turn up the filament current. If I lower the bias, I can saturate the filament, but the emission is too low. I seem to recall a discussion some time ago about whether the filament power on some of the older 2000FX had enough juice to do the job, but I may be mistaken.
I am using a new Denka filament. I have a new titanium wehnelt. It is doing the same as a used and a new Denka filament that I had in using the old SS wehnelt assembly. There are no problems when a tungsten filament is used. My thought is that if another brand of filament requires less current to heat (say about the same as the tungsten filament) that I might be able to use that.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Group, At the upcoming M&M Conference in Long Beach (5/9 August), Microscopy Today will again hold a "Just For Fun Micrograph Contest". Prizes will be $300, $200 and $100 respectively for first second and third prizes. If interested in participating, kindly contact me off line and I will provide complete information. See you in Long Beach, Don Grimes, Microscopy Today
I was wanting to know whether anyone has used methyl salicylate to clear fixed slices of plant tissue, and if so, what in their experience is the best method to use.
a colleague would like to simulate HRES images to compare with those recorded using our JEOL 2000fxII TEM. For this he needs to know the objective lens spherical aberration coefficient (Cs). The polepiece we have is the AHP20L. Does anyone know what the Cs value should be for this instrument?
Another parameter he requires is the beam semi-convergence (in mrad). How is this parameter defined and how can we measure it?
I would appreciate any advice you can offer. Cheers,
Mark Blackford TEM Group Materials Division, ANSTO PMB 1, Menai, N.S.W. Australia 2234
Phone 61 2 9717 3027 Fax 61 2 9543 7179
Disclaimer: The views expressed in this E-mail message do not necessarily represent the official views of ANSTO from which this message was conveyed.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Microscopists, } } I am looking for a nice glue to fix the specimen to the tripod polisher for } wedge polishing. The "super glue" stuff does not work well. The specimen falls } off too easily. } } I would appreciate very much if anyone could send me some good idea. } } Best Regards } Csaba Cserhati } Can you fix your specimen with epoxy resin?
I like to glue specimens with epoxy resin because the substance does not harden too fast, and you always have time to find the best location for your sample, so that the latter does not break.
I once read a study which claimed 100% was better that the usual 70%. I cannot remember the authors etc.
Dave
On Tue, 27 Feb 2001 21:39:29 +1000 Jim at ProSciTech {jim-at-proscitech.com} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } If those cells were for TEM sections, I would be very concerned and re-hydrate } them back to 70% alcohol immediately. For SEM lipid extractions matters less } but you may well get some shrinkage. Its less urgent, but I would take those } cells back to 70%. At that and refrigerated you SHOULD see no real change for } some weeks. } Cheers } Jim Darley } ProSciTech Microscopy PLUS } PO Box 111, Thuringowa QLD 4817 Australia } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com } Great microscopy catalogue, 500 Links, MSDS, User Notes } ABN: 99 724 136 560 www.proscitech.com } } On Tuesday, February 27, 2001 7:56 PM, Bart De Pauw } [SMTP:Bart.DePauw-at-rug.ac.be] wrote: } } } } Hello Everyone, } } } } This week, our CPD was broken. I was planning to CPD some samples that } } day. The samples are now in acetone. My question : how long can these } } samples stay in acetone ? The samples are epithelial cells from duodenum } } grown on glass. } } } } greetings, } } } } De Pauw Bart } } Belgium } } } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Mark A long time ago I needed to clear corn roots with attached parasitic roots to make it possible to trace vascular connections between host and parasite. If I remember correctly the process I used employed xylol instead of salicylate:
Decolorize and soften material in 1% hypochlorite bleach or in 0.5M NaOH. Time (hours to days) and choice of bleach or alkali depends on thickness and colour of the material.
After material is sufficiently translucent, dehydrate in ethanol to 100% and clear by immersing in xylol (take all precautions with this). My recollection is that some materials became glassy clear, to such an extent that it was necessary to stain the vascular bundles with a trace of Safranin O during the 100% ethanol step.
Jan
MARK JEFFREY TALBOT wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi, } } I was wanting to know whether anyone has used methyl salicylate to clear } fixed slices of plant tissue, and if so, what in their experience is the } best method to use. } } Thanks in advance, } } Mark Talbot.
-- Prof Jan Coetzee Lab for Microscopy and Microanalysis University of Pretoria, South Africa. Tel: 012-420-2075, Fax 012-362-5150 www.up.ac.za/academic/electron/emunit1.htm
I am interested in a SEM that automatically can detect and quantify the number and chemical composition of inclusions present on a polished steel surface.
The SEM should manage to scan an area automatically and detect all the inclusions, which fulfill some user defined requirements e.g. a size over a certain limit or a special chemical composition
Does anybody have experience of such system and what manufactures are there ?
The Society of Electron Microscope Technology (SEMT), is holding a one day meeting in London (England) on 28th March 2001 to include the following topics:
Cryo TEM Low Vacuum/Low Voltage Microscopy GFP Stereology Confocal and 2-Photon Microscopy Digital Imaging in Microscopy
All are welcome and further details are shown on the attachment. As a committee member I can supply more information but it might be quicker to go direct to the secretary David McCarthy, e-mail address also on the attachment,
Most EDS manufacturers offer this function as a part of their software. Defining the inclusion positions is a key issue. Most often this is done using BSE imaging by choosing a grayscale range (from histogram) of inclusions while excluding the matrix. It may be an option for some. The most simple method uses beam sweep control by the EDS/imaging system. Some "integrated" EDS/SEM systems may also offer stage control. Haven't looked in detail at the various manufacturer's specs for a while, so hesitate to be more detailed. They, I am sure, would be glad to give you the details!
Woody
} ---------------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } } I am interested in a SEM that automatically can detect } and quantify the number and chemical composition of } inclusions present on a polished steel surface. } } The SEM should manage to scan an area automatically } and detect all the inclusions, which fulfill some user defined } requirements e.g. a size over a certain limit or a special } chemical composition } } Does anybody have experience of such system and } what manufactures are there ? } } Best regards } Joakim Fagerlund } } } } }
I agree with Scott (below). I've mangled a number of samples, in various ways, learning the tripod technique but have never had a complete (rare partial de-wets can be repaired) glue failure using the Loctite product. It's available in a "pen" type applicator which seems to have the best bench life of any of the applicators I've used. My tube usually is swiped off the bench long before it goes bad. One of the advantages to the cyanoacrylic cement is it dissolves, in a reasonable time, in acetone. If you were to use a crosslinked epoxy, you'll need to devise a cleaning scheme that will exhaustively remove the epoxy without altering your sample. Good luck.
John
John W. Heckman MSM Department 3505 Engineering Building Michigan State University East Lansing, MI 48824-1226 heckman-at-pilot.msu.edu
517-353-9719 (work) 517-353-9842 (dept. fax)
"Walck, Scott D." wrote:
} } There has been a lot of discussion over the past on this subject. The boys from IBM are the experts. (There's your plug, Ron.) Since nobody has answered this yet, I'll give you my summary. Ron, If I say anything wrong, please correct me. } } Don't use any type of super glue gel! It doesn't work, you have to use the thin stuff. } } The IBM folks (Ron et al.) recommend Ross super glue, but that is sometimes difficult to locate in other parts of the country. } } I have had good success with the Alpha superglue from Electron Microscopy Sciences and with Loctite superglue available almost anywhere. Buy it from a place that has a high turnover rate of it so that it is fresh. The IBM guys recommend keeping the stuff unopened in the refrigerator for any length of time. Once it has been opened, you can't use it for very long (a day or two). I have used the Loctite a little longer because it has a very good sealing top. } } I think that pressure on the sample while it cures is important, but the IBM guys just wick the extra stuff away from the sample and don't put a lot of pressure on the sample. } } -Scott } } Scott D. Walck, Ph.D. } PPG Industries, Inc. } Glass Technology Center } Guys Run Rd. (packages) } P. O. Box 11472 (letters) } Pittsburgh, PA 15238-0472 } } Walck-at-PPG.com } } (412) 820-8651 (office) } (412) 820-8161 (fax) }
I responded to the requester off line and said that most any super glue works well. I should have added that "super glue" is defined as a "cyanoacrylate," which should be printed on the bottle. We've never tried a "gel' but if Scott doesn't like it that's good enough for us. The main emphasis of my response was the absolute requirement for a clean surface for the glue to bond to. Lack of cleanliness is the biggest cause of specimens "falling off too easily." The bonding surfaces of the tool and the specimen *must* be free of any contamination films. Inspect the surfaces with reflected light. Water from the tap and impure solvents are to blame.
With regard to the glue. We stopped using Ross awhile back. Currently we have Loctite and ND Industries Super Glue in the lab.
Ron
Don't use any type of super glue gel! It doesn't work, you have to use the thin stuff.
The IBM folks (Ron et al.) recommend Ross super glue, but that is sometimes difficult to locate in other parts of the country.
I have had good success with the Alpha superglue from Electron Microscopy Sciences and with Loctite superglue available almost anywhere. Buy it from a place that has a high turnover rate of it so that it is fresh. The IBM guys recommend keeping the stuff unopened in the refrigerator for any length of time. Once it has been opened, you can't use it for very long (a day or two). I have used the Loctite a little longer because it has a very good sealing top.
I think that pressure on the sample while it cures is important, but the IBM guys just wick the extra stuff away from the sample and don't put a lot of pressure on the sample.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
-----Original Message----- From: Csaba Cserhati [mailto:cserhati-at-delfin.klte.hu] Sent: Tuesday, February 27, 2001 4:22 AM To: Microscopy-at-sparc5.microscopy.com Subject: Tripod Polisher
--------------------------------------------------------------- --------- The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
I am looking for a nice glue to fix the specimen to the tripod polisher for wedge polishing. The "super glue" stuff does not work well. The specimen falls off too easily.
I would appreciate very much if anyone could send me some good idea.
I would like to ask some basic things of those cameras that scan the CCD to get more resolution than the specific CCD really offers. I can understand that this can work with color CCDs that have the color mask (array or mosaic or whatever it is called) on the chip and cannot reach the full resolution of the CCD. However, according to my knowledge the "scanning" cannot make the resolution better than with the same chip used as black-and-white camera (without the color mask). So the scanning is done just to compensate against the loss of resolution caused by the color mask. Or has somebody really invented some new rules. Could somebody please advise me, if I have missed something here.
-----Original Message----- From: Hisashi Okugawa [SMTP:okugawa.h-at-nikon.co.jp] {mailto:[SMTP:okugawa.h-at-nikon.co.jp]} Sent: Tuesday, February 27, 2001 12:18 PM To: Giles, Bill; Gary Gaugler Cc: MSA listserver Subject: Re: Metallograph and Digital Camera for Light Microscopy
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html {http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}
Nikon DXM1200 has 1.3M pixels CCD. With 3 x 3 times "Diractor", it makes 12M pixels images. Because of the color array, 2 pixels should be moved totally. DXM1200 moves two times every two thirds pixels. Pixera moves 1 time every one pixel or three times every half pixels. Please visit the following web to get more information
Hisashi Okugawa 1st design department Instrument company in Nikon Corporation Phone: 81-45-853-8568 Fax: 81-45-853-8475 e-mail: okugawa.h-at-nikon.co.jp {mailto:okugawa.h-at-nikon.co.jp} ----- Original Message ----- } From: "Gary Gaugler" {gary-at-gaugler.com {mailto:gary-at-gaugler.com} } To: "Giles, Bill" {William.Giles-at-TIMET.com {mailto:William.Giles-at-TIMET.com} } Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com {mailto:Microscopy-at-sparc5.microscopy.com} } Sent: Tuesday, February 27, 2001 6:52 AM Subject: RE: Metallograph and Digital Camera for Light Microscopy
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html {http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } -----------------------------------------------------------------------. } } } The Nikon MX1200 is native 1.2M pixels. It, like current } generation digital microscope cameras, do a "mechanical" } interpolation to increase final pixel count. One way is } to physically move the CCD imager. Each movement } results in new image data for that position. This is a bad } way to gather more pixels, however. The other way is to } keep the CCD fixed but scan the image across it multiple } times at different angles. } } Zeiss has tried to do mechanical shifting and not done } all that good of a job. Looks like Nikon is trying it too. } They are up against a basic patent by Pixera. Pixera } uses what they call a "Diractor" to scan the image } across a fixed CCD. } } http://www.pixera.com/PixeraCatalog/Penguin/Penguin.htm {http://www.pixera.com/PixeraCatalog/Penguin/Penguin.htm} } } The Pixera is available as a 1.5M pixel unit or as a 5.8M pixel } Diractor unit. Each are also available with Peltier cooling. } Like the Nikon, the Pixera uses a dedicated PCI card for } PC or Mac. This card, camera and software will do } live focus at 12-15 fps on a 733MHz system. It does } manual and automatic exposure and also provides a focus } indication bar. Also provided is automatic or manual } white balance, selectable black balance and multi-size } spot exposure metering. } } The best way to find out how well a camera performs is } to take a high resolution shot at different lighting extremes } and do an FFT on the images. Artifacts and garbage will } show up if the system produces high pixel count at the } expense of image quality. } } I use a Pixera Penguin 600CL and like it very much. It } is easy to take single shots either as one exposure or } averaged or integrated up to 256 exposures. Averaging } and Peltier cooling greatly reduces ultimate noise in the } image. For simple documentation purposes, this is } not necessary. But for images which will undergo } further processing, noise and artifacts must be reduced } or eliminated. The Pixera has selectable ISO from 50 to 400. } I run it at ISO 100. No info on what the Nikon does. } } The Pixera also supports Twain interfacing. } } gary g. } } At 08:57 AM 2/26/01, you wrote: } } } Lawrence, } } } } Thanks for your response. } } } } I read the literature available on the indicated web site and have a couple } } of questions. } } } } There seems to be some conflicting information. } } Under specifications: } } Image size 3840 x 3072 } } CCD 1.34 M pixels } } Document text: 12 million pixels (3046 x 3072) ??????? } } } } Is my math wrong or am I missing some pertinent information? } } } } How do you obtain RGB? What does the color array on the chip look like? Or } } does the camera use filters? } } } } What's the s/n ratio? } } } } Pixel size? } } } } Are there twain drivers available or a Photoshop plugin? } } } } Is any annotaion, image enhancement and/or image analysis software included? } } } } BTW, the Spot RT is $7k also, not twice that price. } } } } William T. Giles } } Sr. Electron Microscopist } } Met. Lab. Coordinator } } Henderson Technical Laboratory } } TIMET } } PO Box 2128 Henderson NV 89009 } } Ph: (702)566-4436 } } Fax: (702)564-9038 } } E-mail: Bill.Giles-at-timet.com {mailto:Bill.Giles-at-timet.com} } } } } } -----Original Message----- } } } From: Lawrence Kordon [SMTP:nikon-at-jagunet.com] {mailto:[SMTP:nikon-at-jagunet.com]} } } } Sent: Friday, February 23, 2001 1:38 PM } } } To: Giles, Bill } } } Cc: 'Microscopy-at-MSA.Microscopy.Com' } } } Subject: Re: Metallograph and Digital Camera for Light Microscopy } } } } } } William, } } } } } } Excellent choice on the Nikon Epiphot 200! However, why are you not } } } considering } } } the Nikon DXM1200 Digital Camera? It is a whopping 12 Million pixels with } } } a live } } } Video Output to a PC and cost under $7000. That is about the half the } } } price of } } } the Spot-RT. Also, why would you want to have a cooled CCD anyway? } } } Metallurgy; } } } even with Fluorescence and high Mag DIC (Pol) does not require it. Not to } } } mention this camera can do extremely low light anyway! This Camera is } } } literally } } } the hottest product in Microscopy today! We have sold hundreds already } } } "sight/unseen" and are first shipping them. All the major Image Analysis } } } companies have or are writing Drivers and it is so popular we have put it } } } on } } } other brands microscopes as well! By the way, the US MINT and some NIST } } } Labs } } } here is Washington, DC have it. Please check out the specs at.... } } }
} } } 1200 } } } and call you local Nikon Dealer for a Demonstration and Quotation. } } } } } } Good Luck, } } } } } } Lawrence Kordon } } } Nikon Instruments, Inc. } } } Columbia, MD } } } nikon-at-jagunet.com {mailto:nikon-at-jagunet.com} } } } } } } } } } } } } "Giles, Bill" wrote: } } } } } } } ------------------------------------------------------------------------ } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html {http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } } } } -----------------------------------------------------------------------. } } } } } } } } Good Day Listers, } } } } } } } } I am in the wonderful position of purchasing a new inverted metallograph } } } } with digital image acquisition for our laboratory. } } } } } } } } I have pretty much decided on the Nikon Epiphot 200 metallograph with } } } } brightfield and polarized light modules. Also chosen were the Plan } } } Achromat } } } } objectives. Any comments on this choice? } } } } } } } } Now the real question. Which digital camera would you buy? I know these } } } } things have been discussed greatly here in the past and I have followed } } } them } } } } with great interest. } } } } } } } } One of our primary wants is live image preview(} 15 fps). } } } } } } } } We are leaning towards the Spot RT, although the Insight looks to be } } } equally } } } } promising. } } } } } } } } Any comments for or against either of these would be appreciated. Also, } } } if } } } } anyone has another preference I would appreciate hearing your comments. } } } } } } } } Thanks } } } } } } } } William T. Giles } } } } Sr. Electron Microscopist } } } } Met. Lab. Coordinator } } } } Henderson Technical Laboratory } } } } TIMET } } } } PO Box 2128 Henderson NV 89009 } } } } Ph: (702)566-4436 } } } } Fax: (702)564-9038 } } } } E-mail: Bill.Giles-at-timet.com {mailto:Bill.Giles-at-timet.com} } }
An Email attachment got through the filter this AM.
Although it was a microscopy relevant posting it still contained an attachment. This message about a meeting in London managed to find it's way through a loop-hole in the filter software. I believe that I have plugged that hole now. (I guess I should thank you Terry, since your posting allowed me to find the loop-hole).
As a safety issue let me remind you that you should scan all Email attachments for viruses before you open them as it is possible for viruses to be attached to messages even if the author did not intend it.
As a safety issue, we "try" to reject all postings having attachments and succeed most times. Please remember to send all your Email messages as simple ASCII text embedded in the body of your message. Do not embedd HTML in your messages as some Email programs (MS Outlook in particular) converts this to an attachment in many situations, if this happens then as an attachment the HTML embedded message will then get rejected.
In case your curious, of the 2, 234 messages have triggered the Filter and been rejected in the last 2 years. The vast majority of these were true junk mail.
Remember if you post a message and it gets caught and tagged by the filter as suspect mail, READ THE REJECTION MESSAGE and then follow the directions so that I can sort out the problem related to your posting . Most of the rejections are true junk mail, but a few of you will get caught by accident, this is the price we all have to pay due to the junk mailers.
I apologize for this message. I have not been receiving messages from the list since the second week in February and I am trying to establish why. Again my apologies for cluttering your email boxes.
____________________________________________ Massimo Sassaroli Dept. of Physiology and Biophysics, Box 1218 Mount Sinai School of Medicine One Gustave L. Levy Place New York, NY 10029 Tel:(212) 241-9512 FAX:(212) 860-3369 email: massimo-at-inka.mssm.edu
On the convergence semi-angle, you can measure this in the diffraction pattern IF you are working close to crossover (i.e. beam at crossover on the specimen). Then, the diffraction pattern will consist of disks, and the semi-angle for convergence is the radius of the disks, which you can convert to angle in radians by scaling with respect to the other reflections in the pattern (when you index, you get the Bragg angle if the lattice parameters are known). This angle will scale with the condenser aperture radius. (For more detail, see O'Keefe and Sanders, Acta Cryst A31 (1975) p. 307.)
It is likely that on a modern TEM you are not working very close to beam crossover on the sample. You may safely assume that the convergence at crossover is an upper limit. However, when you decondense the beam you are producing a situation where incident angle varies as a function of position on the sample, and locally the convergence may be much smaller than the total convergence over the entire illuminated area. In the back focal plane, each disk now contains an image of the sample, which goes together with the previous statement.
If you refocus the diffraction pattern so that each spot is a sharp filament image you will see that decondensing demagnfies the filament, so it is (locally) decreasing the convergence angle significantly. The convergence angle now scales not with the condenser aperture but with the spot size. To measure it you may refocus the diffraction pattern to make each spot an in-focus filament image, then measure the semi-angle of the filament images.
I hope this is not more confusing here than helpful!
Regards,
Wharton Sinkler UOP LLC Des Plaines, IL
} -----Original Message----- } From: Mark Blackford [SMTP:mgb-at-ansto.gov.au] } Sent: Tuesday, February 27, 2001 9:43 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: JEOL 2000fxII aberration coefficients } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi All, } } a colleague would like to simulate HRES images to compare with those } recorded using our JEOL 2000fxII TEM. For this he needs to know the } objective lens spherical aberration coefficient (Cs). The polepiece } we have is the AHP20L. Does anyone know what the Cs value should be } for this instrument? } } Another parameter he requires is the beam semi-convergence (in mrad). } How is this parameter defined and how can we measure it? } } I would appreciate any advice you can offer. Cheers, } } Mark Blackford } TEM Group } Materials Division, ANSTO } PMB 1, } Menai, N.S.W. } Australia } 2234 } } Phone 61 2 9717 3027 } Fax 61 2 9543 7179 } } Disclaimer: } The views expressed in this E-mail message do not necessarily } represent the official views of ANSTO from which this message was } conveyed.
I am interested in doing quantitative stereo microscopy using a TEM and am looking for software that may be helpful in such an endeavor. I anticipate the primary application will be examining the spatial arrangement of linear defects. I have not been able to find a software designed directly for quantitative stereo TEM. I have come across one commercial software that can be used for SEM surface topography that may be applicable. It seems like there must be other software packages out there used for applications such as surface mapping, electron tomography, or even astronomy that could be useful. Also, as I am new to stereo microscopy, any words of wisdom would be appreciated.
I would like to ask some basic things of those cameras that scan the CCD to get more resolution than the specific CCD really offers. I can understand that this can work with color CCDs that have the color mask (array or mosaic or whatever it is called) on the chip and cannot reach the full resolution of the CCD. However, according to my knowledge the "scanning" cannot make the resolution better than with the same chip used as black-and-white camera (without the color mask). So the scanning is done just to compensate against the loss of resolution caused by the color mask. Or has somebody really invented some new rules. Could somebody please advise me, if I have missed something here.
Dear Ari, You are, indeed, missing something. I expect that there is a less obscure referrence, but the one that I know of is a paper by Atsushi Imiya in Vol. 101 (1997) of Advances in Imaging and Electron Physics. The paper, Formal Polynomials for Image Processing, goes from p. 99-142, and includes a section from p. 125-135 on subpixel resolution and pyramid transforms. The author's address is Department of Information and Computer Sciences, Faculty of Engineering, Chiba University, Yayoi-cho, Chiba 260, Japan. If you can't find the volume, you can write for a reprint. Yours,
Bill Tivol Wadsworth Center Albany NY (518) 473-7399 WFT02-at-health.state.ny.us
Could anyone please recommend me a database with manufacturers / suppliers for a polarizing optical microscope with reflectance mode and Nomarski attachment? Any experience of a recent purchase would also be welcome.
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
I can't help you with your Cs value, but the incident-beam convergence semi-angle can be measured from a diffraction pattern taken with the beam adjusted as for imaging. With luck you should see a disk at each diffraction spot [1,2], and the radius of the disk is the semiangle. If you use a specimen with a known d-spacing in a known orientation, you will be able to scale the diffraction pattern in angle -- remember that 2.theta = n.lambda/d and get the semiangle in milliradian. Then you need to decide if the disks have uniforn illumination across them, or if the intensity is greater in the center. If you think that the intensity distribution is more like a 2-D Gaussian, use a HREM-simulation program that uses a Gaussian model for the convergence. If you think the disks are uniform, then use a HREM-simulation program that uses a "top-hat" model for the convergence. If the disks are uniform, but you are stuck with a HREM-simulation program that uses a Gaussian model for the convergence, make sure that the std.dev. of the Gaussian (its sigma) is set to 0.77 times the disk radius [2]. [1] "n-beam lattice images, VI. Degradation of image resolution by a combination of incident-beam divergence and spherical aberration", M.A. O'Keefe and J.V. Sanders, Acta Cryst. A31 (1975) 307-310. [2] "Using convergence and spread-of-focus parameters to model spatial and temporal coherence in HRTEM image simulations", Jan-Olle Malm and Michael A. O'Keefe, in 51st Ann Proc. MSA, Cincinnatti, Ohio (1993) 974-975. Cheers, Mike O'Keefe
Mark Blackford wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi All, } } a colleague would like to simulate HRES images to compare with those } recorded using our JEOL 2000fxII TEM. For this he needs to know the } objective lens spherical aberration coefficient (Cs). The polepiece } we have is the AHP20L. Does anyone know what the Cs value should be } for this instrument? } } Another parameter he requires is the beam semi-convergence (in mrad). } How is this parameter defined and how can we measure it? } } I would appreciate any advice you can offer. Cheers, } } Mark Blackford } TEM Group } Materials Division, ANSTO } PMB 1, } Menai, N.S.W. } Australia } 2234 } } Phone 61 2 9717 3027 } Fax 61 2 9543 7179 } } Disclaimer: } The views expressed in this E-mail message do not necessarily } represent the official views of ANSTO from which this message was } conveyed.
http://www.amc.anl.gov/Docs/NonANL/ComSites.html#Catag } scan down the list to the light/optical/confocal listings.
howwever, this listing is NOT differentiated by the specific criteria you asked. It only represents vendors that have submitted information and is therefore not necessairly comprehensive.
Nestor
} } } } } } } } Could anyone please recommend me a database with manufacturers / suppliers } } for a polarizing optical microscope with reflectance mode and Nomarski } } attachment? Any experience of a recent purchase would also be welcome. } } } } +------------------------------------------------------------------------+ } } | Robert H.Olley Phone: | } } | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | } } | University of Reading {University internal extension 7867 | } } | Whiteknights Fax +44 (0) 118 9750203 | } } | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | } } | England URL: http://www.reading.ac.uk/~spsolley | } } +------------------------------------------------------------------------+
=========================================== Dr. Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov =========================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ===========================================
The box said ... "This program requires Win 95/98/NT or better..." So I bought a G3 Mac !
I'd like to get a closer look on coatings of silica-fibers using SEM. Has anybody ever tried to polish cross- and/or longitudinal sections?
Thanks for any information! Werner _______________________________________________________________________________ Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE: http://web.de Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP: http://freemail.web.de
While the simpler and less time consuming suggestions already mentioned in this thread may work in some cases, I found out a long time ago that the only way to deal with the "fungal jungle", eg. fungi cultured on agar plates, is to hit 'em with the ol' OTO method, preceded by glutaraldehye fixation. Quick outline is as follows:
Glut. fixation, your favorite buffer Buffer rinses Osmium tetroxide, 2% Water rinses Thiocarbohydrazide, sat. soln.,filtered water rinses Osmium tetroxide, 2% Water rinses dehydration series, EtOH, or acetone CPD metal coating? Maybe, maybe not. Try without first.
This eliminates the charging, and fixation is quite good.
Classic reference for this method:
"Ligand-mediated osmium binding:Its application in coating biological specimens for scanning electron microscopy". Kelley, Robert O., A.F. Dekker and John G. Bluemink. J. Ultrastrucute Research 45:254-258 (1973).
See also:
"Non-coating techniques to render biological specimens conductive/1980 update". Judith A. Murphy. Scanning Electron Microscopy/1980 Vol.I, p. 209-220.
You might try exposing your non-aquatic sample (bread molds, mushrooms, etc. ) to vapors of Osmium tetroxide for several hours ( a few drops of 4% OsO4 on the lid of a very small container with your sample inside or the lid over the sample directly), then air dry, mount, coat and view. As with any sample, the drying artifacts vary with the fungus. Some things look great.
good luck
Steve } ----------------------------------------------------------------- } Hello, } } Forgive me, I haven't tried to find this on my own yet... } } But have any of you used an electron microscope to get a } close-up of a fungus? Can this be done? } } Thanks...
___________________________________________________ Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619) 594-4523 fax: (619) 594-5676
-- Gib Ahlstrand Electron Optical Facility, University of Minnesota, CBS Imaging Center, 35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454 (612)624-1799 FAX, giba-at-puccini.cdl.umn.edu http://www.cbs.umn.edu/ic/
You've addressed my concerns exactly. Then again, you're not trying to sell me a camera.
I tend to be wary of 12 megapixel output from a 1.34 megapixel chip, even if "my" math is wrong.
I too am interested in how the interpolation is done in these cameras.
It appears that some manufactures are using controlled optics to shift the image on the CCD, whilst others are shifting the CCD. How that interpolation then happens would be most informative. Here again, real info is difficult to come by on the "sales" oriented sites.Pixera seems to offer more info than others.
It would seem that by using the Bayer mask, which uses 2 green tinted pixels for 1 each of blue and red, that the resolution of the chip would decrease fourfold.
Here again, i'm just an inquiring mind that would like to know how the technology that we, the buying public, are being asked to believe.
William T. Giles Sr. Electron Microscopist Met. Lab. Coordinator Henderson Technical Laboratory TIMET PO Box 2128 Henderson NV 89009 Ph: (702)566-4436 Fax: (702)564-9038 E-mail: Bill.Giles-at-timet.com
} -----Original Message----- } From: Kuusisto, Ari [SMTP:Ari.Kuusisto-at-perkinelmer.com] } Sent: Wednesday, February 28, 2001 6:56 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Nikon DXM1200 and "mechanical" interpolation to increase the } fina l pixel count } Importance: High } } Hi all, } } I would like to ask some basic things of those cameras that scan the CCD } to } get more resolution than the specific CCD really offers. I can understand } that this can work with color CCDs that have the color mask (array or } mosaic } or whatever it is called) on the chip and cannot reach the full resolution } of the CCD. However, according to my knowledge the "scanning" cannot make } the resolution better than with the same chip used as black-and-white } camera } (without the color mask). So the scanning is done just to compensate } against } the loss of resolution caused by the color mask. Or has somebody really } invented some new rules. Could somebody please advise me, if I have missed } something here. }
I'm preparing a talk which is to include a timeline giving dates and names for some relevant TEM developments. I'm trying to find answers to two trivia questions with great importance to microscopy:
1) Who was the first to knowingly image a dislocation in a TEM, when was it done and where was it published? On this, I have found references as far back as an article by Heidenreich in J. Appl. Phys, 1949, but don't have this journal going back that far.
2) Who developed the solid state detector for energy analysis of x-rays (EDS), when and where published?
I have found some references to books on TEM history by Marton (1968) and Hawkes (1985) but don't have these on hand.
Thanks,
Wharton
*********************** Wharton Sinkler UOP LLC Des Plaines, IL
----- Original Message ----- } From: Sinkler, Wharton {WSinkler-at-uop.com} To: {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, March 01, 2001 3:45 AM
This is a forensic microscopy question. I would like a stain(s) that differentiates between skin/protein particles and cellulose/starch particles. I would like the stain to be evident in the compound microscope under normal incident lighting. The particles are filtered from a solution of IPA and captured on a .2um polycarbonate filter. The information I have come across would suggest Ninhydrin for the staining of peptides in skin, but I have not found any info on preferentially staining cellulose/paper products.
1mm? For that I'll use a ruler. One of our stage micrometers is 0.1mm long and has 0.002mm divisions. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Thursday, March 01, 2001 2:53 AM, Seijo, Edward R. [SMTP:SeijoER-at-moffitt.usf.edu] wrote: } } } Looking for a stage micrometer that will allow me to calibrate down to the } micrometer level. Everything I have found thus far is 1mm. } } Thanks
That would be new to me. I would like to see that study. It also would be counter-intuitive since conc alcohol and acetone are known to extract lipids, whereas 50 to 70% hardly does. And since membranes are mostly lipids . . . Also during dehydration the greatest specimen shrinkage occurs above 80% alcohol. So it makes sense to have more and smaller steps at the upper end, but that is mostly due to physical effects. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes ABN: 99 724 136 560 www.proscitech.com
On Wednesday, February 28, 2001 8:01 PM, Patton, David [SMTP:David.Patton-at-uwe.ac.uk] wrote: } } Re what % ethanol to use for storage. } } I once read a study which claimed 100% was better that the } usual 70%. I cannot remember the authors etc. } } Dave } } } On Tue, 27 Feb 2001 21:39:29 +1000 Jim at ProSciTech } {jim-at-proscitech.com} wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } If those cells were for TEM sections, I would be very concerned and re- } } hydrate } } them back to 70% alcohol immediately. For SEM lipid extractions matters less } } } } but you may well get some shrinkage. Its less urgent, but I would take those } } } } cells back to 70%. At that and refrigerated you SHOULD see no real change } } for } } some weeks. } } Cheers } } Jim Darley } } ProSciTech Microscopy PLUS } } PO Box 111, Thuringowa QLD 4817 Australia } } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com } } Great microscopy catalogue, 500 Links, MSDS, User Notes } } ABN: 99 724 136 560 www.proscitech.com } } } } On Tuesday, February 27, 2001 7:56 PM, Bart De Pauw } } [SMTP:Bart.DePauw-at-rug.ac.be] wrote: } } } } } } Hello Everyone, } } } } } } This week, our CPD was broken. I was planning to CPD some samples that } } } day. The samples are now in acetone. My question : how long can these } } } samples stay in acetone ? The samples are epithelial cells from duodenum } } } grown on glass. } } } } } } greetings, } } } } } } De Pauw Bart } } } Belgium } } } } } } } } } ---------------------------------------- } Patton, David } Email: David.Patton-at-uwe.ac.uk } "University of the West of England" }
THIRD ANNOUNCEMENT: UBC LIVE-CELL COURSE: Please register by March 1!!
(Note: I know that today is the deadline but there were still some vacancies last night and we will accept applications as long as this remains true. In addition, there is a Waiting List to replace those who find they cannot come for medical or financial reasons. JP)
Hello all,
We have two additions to the faculty for the 2000 UBC 3D Live-cell Microscopy Course:
* Alan Hibbs from BioCon in Melbourne (who was really on the list to start with but I somehow missed on the previous announcements. Sorry Alan!)
* Mark Cannell from U. Auckland NZ. Well known for developments in multiphoton microscopy but also for deconvolving 3D data from confocal and multiphoton microcopes. Mark will replace Jason Swedlow from U. Dundee who had schedule conflicts (Thanks for your help the last 3 years Jason!)
Other faculty include:
o Stephen Adams University of California-SD o Ping Chin Cheng SUNY, Buffalo o Elaine Humphrey Univ. of British Columbia o Stephan Hell Max Planck, Goettingen o Ted Inoué Universal Imaging, PA o Iain Johnson Molecular Probes, OR o Larry Keenan Cell Robotics, NM o Ernst Keller Carl Zeiss, Thornwood, NY o Andreas Kriete University of Geissen o Felix Margadant University of Sydney o Tim Murphy University of British Columbia o Jim Pawley University of Wisconsin-Madison o Steve Potter California Institute of Tech o Michael Weis Agriculture Canada
Basic info is below but you can get the entire brochure, including links to faculty home pages, at
Organized by Prof. James Pawley, (University of Wisconsin-Madison)
in association with Dr. Elaine Humphrey UBC BioSciences Microscopy Facility: University of British Columbia Vancouver, BC, Canada
DATES
Applications must be received by March 1, 2001 Deposit due April 15, 2001 Registration 5:00 - 7:00 PM Sunday, June 17, 2001 First Lecture 7:30 PM Sunday, June 17, 2001 Live-cell Course ends, noon Thursday, June 28, 2001 3D Image Processing Wksp Sat. June 30 - Monday, July 2
APPLICATIONS DUE BY MARCH 1, 2001
More information at : http://www.cs.ubc.ca/spider/ladic/course/bulletin.html
or
REGARDING COURSE ORGANIZATION:
Contact: Prof. James B. Pawley, JBPAWLEY-at-FACSTAFF.WISC.EDU
REGARDING APPLICATIONS
Dr. Elaine Humphrey, Biosciences EM Facility Biosciences Building Univ. of British Columbia 6270 University Blvd. Vancouver, BC, V6T-1Z4
THE PURPOSE OF THE COURSE
Modern methods of 3D light microscopy promise a revolutionary improvement in our ability to view living cells. To help convert this promise to reality for a wider selection of biological scientists, an intensive eleven-day residential course concentrating on all aspects of 3D Microscopy of Living Cells will be held at the University of British Columbia, in June of 2001. The course includes 4 days on 2D techniques, 6 days of 3D techniques and a summary presentation. It covers basic microscopy to the highest level confocal microscopy. (A half-day Pre-course is offered for any who may need to brush up on basic optics!).
Topics include: o Quantitative 2D light microscopy o How to keep your cells alive o 3D imaging in confocal microscopy o Widefield/deconvolution techniques o Two-photon excitation microscopy o Fluorescent and backscattered light signals o Dye design, characteristics and use o Pixelation: The Nyquist Criterion o Lasers and laser tweezers o Objectives and aberrations o Scanning-systems: AODs and mirrors o Optimal pinhole size/photon efficiency o Detectors: operation and performance o Video-rate confocal imaging o Measuring ion concentrations o Display and measurement of 3D data
Morning lecture/demonstrations lead to hands-on laboratory exercises each afternoon that will utilize most of the commercial instruments currently available for 3D microscopic imaging. Students will work in groups of 3 or 4 throughout the discussion and laboratory sessions, and may complete a live-cell 3D study on their own specimens. In the first five years, over 130 students from 23 countries have attended. Last year, 11 separate, 3D microscopical workstations were available for student use under the supervision of a 17-member international faculty. We expect to have even more workstations in 2001. Including manufacturers representatives, the teacher/ student ratio will be almost 2:1.
INTERNATIONAL FACULTY
o Stephen Adams University of California-SD o Ping Chin Cheng SUNY, Buffalo o mark Cannell Univ. of Auckland o Elaine Humphrey Univ. of British Columbia o Alan Hibbs BioCon, Melbourne o Stephan Hell Max Planck, Goettingen o Elaine Humphrey University of British Columbia o Iain Johnson Molecular Probes, OR o Larry Keenan Cell Robotics, NM o Ernst Keller Carl Zeiss, Thornwood, NY o Andreas Kriete University of Geissen o Felix Margadant University of Sydney o Glen MacDonald University of Washington o Tim Murphy University of British Columbia o Jim Pawley University of Wisconsin-Madison o Steve Potter California Institute of Tech o Michael Weis Agriculture Canada
TUITION
Course tuition is $2,150 US and includes lunches. On receipt of 50% deposit, students will receive preliminary group assignments and the textbook, Handbook of Biological Confocal Microscopy, (Plenum, 1995). The tuition fee includes the textbook, a binder of handouts, and tickets for the Opening Reception, the Manufacturer's Reception and the Beach Party. Accommodations and meals other than lunch are not included in the tuition fee. The Pre-course is $100 US.
APPLICATIONS
Applicants will complete a questionnaire to assess knowledge level and field of interest. Enrollment is limited to about 24 participants. Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with basic texts on request to read before the course begins and are encouraged to take the Pre-course on the afternoon of June 18.
Application forms, and other course information from this and past years, can be downloaded from the WWW site at:
Dr. Elaine Humphrey, Biosciences EM Facility Biosciences Building Univ. of British Columbia 6270 University Blvd. Vancouver, BC, V6T-1Z4 Phone: 1-604.822-3354 FAX: 1-604.265-5315 Email: ech-at-unixg.ubc.ca.
Application deadlines:
Application forms are due by March 1, 2000! Deadlines are early to facilitate setting up groups. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 2000 to reserve your position. In general, deposit refunds are only be possible if your position can be filled from the waiting list. The remainder of the fees is due at registration.
DATES
Applications must be received by March 1, 2001 Deposit due April 15 2001 Registration 5:00 - 7:00 PM Sunday, June 17, 2001 First Lecture 7:30 PM Sunday, June 17, 2001 Live-cell Course ends, noon Thursday, June 28, 2001
TEACHING PHILOSOPHY
As befits teaching in an area at the boundary of "what is now known," lecturers have been chosen based on their expertise as scientists working in the field rather than because they all agree. They are encouraged more to be provocative than to be prosaic. Students should expect discussion in areas where differences of opinion exist.
Prior to the course, students will be organized into groups and encouraged to communicate by email/phone, about the "Living-cell" group projects that they will pursue during the course and that will be presented to the class on the last day of the course. It has been found that group interactions make best use of students' prior experience and can be very effective in teaching the practical skills covered in a hands-on course of this type.
ARRANGEMENTS FOR LIVE SAMPLES
Students must contact Dr. Elaine Humphrey to make necessary arrangements for the transport and maintenance of cell lines etc. needed for their projects. Organisms linked in any way with human disease are not permitted because of safety considerations. Transport and customs arrangements for living specimens are entirely the responsibility of the student. Students also attending the 3D Image Processing Course, may be able to analyze, process and display some of the 3D data collected from their specimens
The workshop will cover 3D image processing for measurement and display. Enrollment is limited to those attending the 3D Microscopy course. Tuition : $850 US (lunches and snacks incl.)
WHO SHOULD ATTEND?
The course is designed for biologists working with multidimensional and possibly multicolor microscopical data sets. Getting the data is only half of the battle. Image data in 3, 4 or even 5 dimensions may be difficult to store let alone analyze or display. This course is to help students understand the hardware and software aspects of this problem and give them the techniques they need to make the best use of their data.
The course is designed for biologists who need to make measurements on 3D microscopical data sets and then display the results in an effective manner. The course will be taught on SGI, Macintosh and IBM-compatible computers. A wide variety of software designed for the 3D microscopy market will be described, demonstrated and available for use.
Workshop Organizers
o Andreas Kriete Giessen University, DE o Felix Margadant University of Sydney, Au o Ping Chin Cheng State U. of New York, Buffalo o Elaine Humphrey University of British Columbia o Glen MacDonald University of Washington
PLAN OF INSTRUCTION Classes will meet from 8:30-12:00 and 1:00-6:00 with lecture-demonstrations followed immediately by hands-on laboratory sessions using a variety of workstations. Students will "learn-by-doing" with two to a machine. Lab handouts will describe some specific exercises to be performed on "canned" data sets. Facilities and supervision will be available until 11:00 PM, for students to work on their own data. -- **************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39
$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$ From daemon Sat Mar 3 12:46:08 2001
I would like to thank those colleagues who kindly provided their advice and shared their experience with us regarding the problems of vibratome brain sectioning that I posted on the list server a while ago.
We now use double-edge feather blades that we purchased from Ted Pella, the quality of sections are much improved, also sometimes it seems helpful at low amplitude rather than high one.
Xinran Liu, M.D., Ph.D.
Center for Basic Neuroscience UT Southwestern Medical Center 6000 Harry Hines Blvd., NA4. 214A Dallas, TX 75390-9111
Arron: Try using Araldrite 502 for your resin. It does not contain NMA. This should eliminate your concern with using KMnO4. The mixture I use is a 1-1 Araldrite 502 and DDSA, mix well then add your DMP-30 and mix again. Don't worry about the air bubbles.
Ron Austin (Research Associate) Dept of Pathology LSU Medical Ct. Shreveport, LA rla-at-mindspring.com
-----Original Message----- } From: "PostMaster {rla-at-mindspring.com} "-at-sparc5.microscopy.com [mailto:"PostMaster {rla-at-mindspring.com} "-at-sparc5.microscopy.com] Sent: Saturday, March 03, 2001 2:43 PM To: rla-at-mindspring.com
-----Original Message----- } From: Ronald Austin [mailto:rla-at-mindspring.com] Sent: Saturday, March 03, 2001 2:41 PM To: Microscopy Society of America
Email: pjp6-at-dana.nau.edu Name: Pete Polsgrove School: Northern Arizona University
Question: I'm looking for an protocol for imaging fecal pellets. The pellets are from a hydrobiid snail found in highly mineralized warm desert springs and Posos in Mexico. I am interested primarily in the bacteria found on and in them but would also like to be able to inventory the entire contents of the pellets to help get a better picture of what part they play in their isolated ecosystem. I've done some light and SEM on samples but need to get inside to determine if the bacteria that break them down are carried internally or are picked up environmentally from the water. For sample preparation and sectioning I need to consider other contents like diatoms and stromatolite pieces and the high concentration of mineral solids in the samples. To start I plan to fix with glut/OsO4, dehydrate with acetone, infiltrate with propylene oxide and go into embed 812. After sections are imaged for non organic I planned on staining with lead citrate and comparing. Being new to TEM and not having a good idea of the precise osmolarity of the sample has left me puzzling over buffer selection (sodium cacodylate vs. phosphate), concentration, and times. We have an excellent EM technician here at NAU but it seems more my responsibility to make these determinations so any suggestions or references you could offer would be greatly appreciated. Thanks Pete Polsgrove
1) The camera is of course intended for general purpose photography. Snapshots, indoors and outdoors. It has a miniature flash which is good for about ten feet distance. For microscopy, of course the flash is turned off.
2)There are some macro zoom situations where the camera does a nice job. But for compound transmitted and reflected work, I have not found it to be satisfactory. Optem has made a high quality interface for the CoolPix which interfaces to Zeiss, Olympus and many other 'scopes. I have a common optical tube with C-mount to CoolPix for Axioskop and Olympus. I cannot fault this adapter system at all. But as you point out, the native CoolPix optics are not intended for discriminatory microscopy work. At about $700 for the Optem adapter, the CoolPix package with remote release and AC adapter is not cheap.
I recently took 765 pix with the CoolPix on a trip to Australia. It did a super job. I used two 128MB AVL CF modules. Some images were in highest JPEG resolution and some were in XGA fine. All print on an Epson Stylus Photo 2000P with outstanding results. And, of course, they go to the Web without any problem.
The Pixera Penguin 600CL is quite a different camera. It is highly suitable for microscopy, but also will do excellent macro work with an inexpensive C-mount zoom lens. Optem also makes the adapter for this camera to the Zeiss and Olympus microscopes. Same high quality and fine results. I have done multiple slices and stitched them together for a final TIFF file size of 110MB.
The Pixera is non-interpolated 5.8M pixels using their Diractor prism device. At 2776x2074 pixels, it generates a 17.5MB TIFF file. In 16-bit mode, the size is twice that. As I mentioned in a prior post, their software supports multiple capture averaging and integration. It also does variable sized spot metering and average metering. The CL version has Peltier cooling and exhibits exceptionally low thermal noise. Four frame averaging reduces noise even further.
The Pixera is 10-bits per color while the Nikon MX1200 is 8-bits per color. The word on the street is that the next release of the MX1200 will be 10-bits per color. Whether this is worth anything to a particular user is their own decision.
Being a professional Nikon shooter for many years, I am no longer an intransigent fan. In fact, I have dumped nearly all of my Nikon SLR equipment in favor of Contax. This of course has nothing to do with microscopy. But the point is that in my extended experience, Nikon is struggling to develop new products, which by specifications, leapfrog their competition--while in practice, they do not.
Try before buy is a good operative in this respect. I really don't care who makes what, as long as it is good stuff. Sorting through all the marketing hype is a chore. Sometimes even working with the equipment is a chore. But the actual results of using products is much more telling than sales pitches or product brochures.
gary g.
http://photoweb.net
At 06:54 AM 3/3/01, you wrote:
} Gary, } } let me make two observations and see if you agree: } } 1) The Coolpix (and any other camera like it) is originally designed for } snapshots. That means, that the emphasis is on "nice" pictures under normal } (outside or flash) conditions. This does not necessary mean, that one can } take good scientific images. This may be the reason that you had problems } with exposure time. The camera may take a weighted measurement to calculate } exposure time, for example only from the middle. If you take a snapshot } outside, the most important feature is likely to be found somewhere around } the center of the image. So it's important to get that part right. That } might not apply to photography on a microscope. } } 2) The quality of the picture is affected by the weakest link in the chain. } My suspicion is, that the lens on the camera is far inferior to anything you } put on a microscope. For example, we spend a lot of time (and money) to } develop and make our own lenses for our TEM cameras. I don't see the point } of spending thousands of Dollars on good microscope lenses and then have all } that negated by an inferior lens on the camera. } } One word to Nikon: I am not trying to knock this camera, it is probably a } very good camera (I don't know it myself). My remarks are general and apply } to other camera manufacturers (Kodak, Canon, Fuji, etc.). This was just a } thread which mentioned the Nikon camera. Plus, all these ramblings are my } personal opinion. } } Michael Bode, Ph.D. } Soft Imaging System Corp. } 1675 Carr St., #105N } Lakewood, CO 80215 } =================================== } phone: (888) FIND SIS } (303) 234-9270 } fax: (303) 234-9271 } email: mailto:info-at-soft-imaging.com } web: http://www.soft-imaging.com } =================================== } } } } -----Original Message----- } } From: Gary Gaugler [mailto:gary-at-gaugler.com] } Sent: Friday, March 02, 2001 10:20 PM } To: Leonardo Lagoeiro } Cc: MSA listserver } Subject: Re: Nikon CoolPix 990 } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } Subject: } } } } SEM views of gold paint } } } } Content: } } } } Does anyone have any views of modern gold flake (real gold, not brass } } flake) used in making paintable substitutes for gold leaf? } } } } John Twilley } } Conservation Scientist }
VACANCY FOR POST-DOCTORAL RESEARCH SCIENTIST AT AGFA-BELGIUM
In a world in which nanostructured materials are of growing importance for a variety of industrial applications, Agfa’s R&D laboratories are increasingly oriented towards research on nanophased systems. In order to decrease development time for new products based on functional nanomaterials, a significant part of the research effort is spent on an understanding of the basic working mechanisms in a variety of nanostructured systems.
To reinforce this research-team, Agfa-Gevaert N.V. in Mortsel (Belgium) has a 2-year vacant position for a postdoctoral scientist who will be involved in an advanced characterisation programme of nanostructured building blocks for new imaging and other functional materials, including photo-, electro- and thermoresponsive systems. The objective is to use advanced microscopical and analytical equipment to characterise nanoscaled systems, with the aim of providing an increased understanding in the relationship between nanostructuration and properties. Moreover, this research work will also provide clues for a controlled synthesis of nanostructured systems.
The candidate will be working in a diverse industrial R&D-environment with several links to university labs. He/she will be involved in a multidisciplinary team working on several nanostructured applications. Main focus of the work will be on microscopical and crystallographic (diffraction) techniques. In order to meet the technical requirements, the candidate should have a PhD in materials science, experience with TEM- and diffraction-techniques, and have a background in crystallography of inorganic materials. He/she should be a good teamplayer to function in a multi-disciplinary team, and be fluent in English and/or Dutch.
Candidates please apply to the following persons :
Dr. Christiaan Van Roost R&D Materials/Physics and Analytics Septestraat 27 B-2640 Mortsel Belgium Phone : (32)03 444 37 00 E-mail : christiaan.vanroost.cv-at-belgium.agfa.com
For selection procedures, eligibility criteria and so on, please see homepage Marie Curie Industry Host Fellowships: http://www.cordis.lu/improving. Please note in particular that candidate fellows must be nationals of an EU Member or Associated State, or have resided in the EU for at least five years immdiately prior to their selection by Agfa.
I don't want to wake up a recent discussion (I was away in the last weeks), but I take advantage of this subject to askh a question about an other aspect of this subject. Sometimes you have so old material and maintenance services are laughing when they see what your are working with and/or on the other hand (with new materials also) it's faster and cheaper to do cleaning and (basic) maintenance yourself (and I like to do it myself !).
My question : What kind of grease/oil, where, on a LM ?
Manufacterer and maintenance services don't like to answer such a question. They say its complicate, there are much type of greases etc etc... So, does someone know something about that.
Thanks
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess 67037 Strasbourg CEDEX France
I was quite surprised to see the problems that are being discussed in relation to TEM focus, particularly as I was with Hitachi in the HU11 days, lets keep it simple and try to help?
When setting the image focus you should be aware of a number of problems that may complicate matters.
1. To try to focus at condenser cross over is absolutely the wrong thing to do! i) At crossover there is the poorest illumination coherence, this will lead to soft images, not out of focus but not optimised either. ii) At crossover there is a space charge effect (too many electrons in a small area and they repel each other) which may cause a false focus. (Just look at any image at crossover and then overfocus the condenser - you can see more detail!) iii) To focus at crossover and then change to a lower intensity is asking for trouble as the change in heating effect is likely to cause specimen drift and flexing which may change the contrast of material science specimens.
2. You MUST focus at the photographic magnification. Focus is the matching of the focal lengths of the objective and diffraction lenses. If you focus at a higher level and there is a diffraction lens change when dropping the magnification, you will find a matching objective correction will be required or your image will be out of focus.
3. What is best FOCUS, not always true focus? To a materials scientist it will almost certainly be true focus, no Fresnel fringes, as determined by the focus wobbler. They will set their intensity in advance as an intensity change may cause the material to flex and change in contrast. To the biological scientist it will be a degree of underfocus determined by the specimen's organelle density, its thickness, the kV, the objective aperture size and most important, the magnification; this is optimum under focus, the defocus where the (white) Fresnel fringes enhance the high contrast areas!
When taking a photograph the ideal conditions (as used in all the manufacturer's demo laboratories that I have worked with, Hitachi, JEOL and ISI) require the photograph to be taken at the same intensity level as when the focus is set. WHY? Well, once an intensity is set the operator may focus and during the focus procedure they should be concentrating sufficient to spot any image drift; it is most important to see the image under the conditions that you are going to photograph. So what will the excuses be?
1. "It is too dim to focus" - then i) increase the emission current, many people run at too low a current and make the task of optimising the instrument settings much more difficult ii) increase the kV which would increase the intensity too ii) re calibrate the photographic system to allow a focus intensity that is suitable for most operators
2. "The negatives are to dark" - i) the denser the negative the higher the contrast, so may be you could increase the kV, have a more stable specimen and an even brighter image?
3. Difficult one this - "the pathologists like a very bright image" - yes I know I guess it comes from looking down light microscopes all day? i) try 1 or 2 above - "yes but the pathologists like a high screen contrast" - yes I know but you should explain that no one publishes the screen, it it the photographic record that is the most important item(?) and we can get stacks of contrast from modern printing/publishing systems.
Regarding the reported overlap in currents between condenser and objective it is true, however the result is usually more of an image shift than a focal change in my observations, but yet another reason to focus and stigmate at the photographic intensity.
So to conclude I would
1. Always focus and stigmate at the photographic illumination level, if a biologist determine and plot out the optimum under focus for your material over your normal magnification range. 2. Always use the second condenser overfocus from crossover for high coherence and combine this with a 2 micron spot size for biology. 3. Set up the instrument's photographic procedure to suit as many operators as possible so that they too may focus at the photographic level. 4. Increase the emission current to give a higher brightness making point 2 far easier to use 5. Always use a higher kV (if 60, wow please go to 80, if 80 try 100) and work to obtain the best results on a photograph not trying to optimise for the screen. 6. To focus at low magnifications try removing the objective aperture and focus without a binocular, or a wobbler, looking for the very strong minimum contrast effect.
Well I hope this helps, its standard information on our courses, guess we do too much SEM in the States!
Regards
Steve Chapman Senior Consultant Protrain For consultancy and training by professionals World Wide Tel +44 1280 814774 Fax +44 1280 814007 www.emcourses.com
} } I have several questions for an AFM microscopist; } } 1. Can AFM really measure surface modulus?
Probably - but there are a number of factors which may have to be taken into account. These will include the nature of sample, any additional interactions between the sample and the scanning probe tip, the nature of the tip and SPM cantilever and so on.
} } 2. If yes, what is the mode of operation or measurement? } In terms of imaging modes (i.e. those where you are obtaining an image along with surface property information) there are two that may provide some surface modulus information.
i) Phase imaging (see for e.g.http://www.di.com/AppNotes/Phase/PhaseMain.html) is capable of providing a surface map based on the viscoelastic properties of the surface. Therefore the image will probably be a convolution between both the surface modulus and other surface properties (for example elasticity and whether the sample has any interactions with the tip). In this mode the probe is tapped into the surface and the lag between the voltage driving the tip oscillation and the actual tip oscillation provides this information. (It is particularly difficult, if not impossible to quantise the results obtained).
ii) Pulsed force should be able to deconvolute between sample stiffness and sample adhesion. http://www.thermomicro.com/tech/modes/pfm.htm should provide more information about this mode. However whether true values could be measured would again be a matter of some discussion.
Further mode of scanning probe microscopes are the spectroscopic ones in which the probe tip is move towards and away from the sample and the tip response measured. In AFM this mode is force-distance (see for e.g. http://www.thermomicro.com/tech/modes/fvsd.htm). This should again provide some information regarding the surface modulus. And with some tip calibration may provide something akin to a quantitative measurement.
With all of these mode the cantilever type will have an effect on the amount of information available. If the cantilever is less stiff than the surface of interest your measurement will generally be of the Young's modulus of the cantilever.
Nanoindenters may provide a more accurate measurement. http://freespace.virgin.net/micro.materials/ is an example. There are some companies that offer methods of converting commercial SPMs towards a nanoindenter.
} 3. Is it a quantitative measurement? Is it an absolute measurement } or a relative measurement?
My cynical opinion would be that most measurements available from a more qualitative than quantitative, though many would disagree. For example in this case, the area of tip-sample contact is unlikely to be able to be measured accurately but will have a major effect on your measurements, You may however be able to obtain some numbers that can be converted to an absolute measurement after careful consideration.
} } 4. Can the results be correlated to the (bulk) modulus measured by } rheometer?
This would, I presume, depend upon the material.
I hope this is of some use.
Giles
---------------------------------------------------------------------------- --------------------------- Dr. Giles Sanders Zeneca / SmithKline Beecham Centre for Analytical Sciences Chemistry Department Imperial College of Science, Technology and Medicine London SW7 2AY