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From: Dmrelion-at-aol.com
Date: Sat, 1 Sep 2001 06:51:29 EDT
Subject: spectroscopy and spectrometry

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I have followed this discussion with some interest. A similar situation
exists in the field of mass spectroscopy.

At one time there were many mass spectrographs and mass spectrometers being
used. Mass spectrographs were those instruments in which the detected ions
were spread out along a focal plane and a significant portion of the mass
range was collected simultaneously on a photographic plate. Mass
spectrometers were those instruments in which the detected ions were moved
across a detector slit (by varying magnetic field or ion accelerating
potential) and collected/detected, one m/q at a time, by either a Faraday
cup or electron multiplier after the slit, providing a readout on an
electronic device (electrometer usually). Mass spectroscope or mass
spectroscopy was used as the general term to include either type of
instrument.

Mass spectrographs are rarely seen any more and even if they are used, the
photographic plate would probably be replaced by some type of CCD detector
providing electronic readout so the original clear distinction becomes
blurred.

I'm sure that an analogous situation exists in optical spectroscopy.

Don Marshall



Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Sat Sep 1 07:17:06 2001



From: Dmrelion-at-aol.com
Date: Sat, 1 Sep 2001 08:10:07 EDT
Subject: spectrometers and spectroscopes

Contents Retrieved from Microscopy Listserver Archives
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I have followed this discussion with some interest. A similar situation
exists in the field of mass spectroscopy.

At one time there were many mass spectrographs and mass spectrometers being
used. Mass spectrographs were those instruments in which the detected ions
were spread out along a focal plane and a significant portion of the mass
range was collected simultaneously on a photographic plate. Mass
spectrometers were those instruments in which the detected ions were moved
across a detector slit (by varying magnetic field or ion accelerating
potential) and collected/detected, one m/q at a time, by either a Faraday
cup or electron multiplier after the slit, providing a readout on an
electronic device (electrometer usually). Mass spectroscope or mass
spectroscopy was used as the general term to include either type of
instrument.

Mass spectrographs are rarely seen any more and even if they are used, the
photographic plate would probably be replaced by some type of CCD detector
providing electronic readout so the original clear distinction becomes
blurred.

I'm sure that an analogous situation exists in optical spectroscopy.

Don Marshall



Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Sat Sep 1 10:29:40 2001



From: Beauregard :      beaurega-at-westol.com
Date: Sat, 01 Sep 2001 11:18:30 -0400
Subject: Re: TEM - embedding silica

Contents Retrieved from Microscopy Listserver Archives
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Hi Pippa,

You do not state the exact type of silica you are embedding. SO...

In general methacrylates, whether LR White or such, will shrink much more
than an epoxy like one of the EPON CLONE formulations. If I recall 20-25%
shrinkage is what you get with LR White and a lot of polymers. Epoxy
formulas are more like 5%. This lower shrinkage is preferred by me so that
I can argue to customers that I have not changed the microstructrure
through severe shrinkage during the polymerization. LR White is faster
(with an accelerator) curing, if you are in a hurry. The EPON clone stuff
takes overnight curing in an oven at 60 to 70 C. It's no big deal because
you just prepare the samples at the end of the day and start sectioning in
the morning.

I have personnaly done precipitated silicas, arc silicas, and fumed silicas
using Epon. Silica fume should be no problem. I do not vacuum embbed
these materials. Ppt'd silicas have a surface area of about 154-250 meters
squared per gram and the epon will wet almost every pore between the
primary or ultimate particles without vacuum. Capillary forces are strong
enough on our products and others to wet almost 100% of what is there. One
of the components in my EPON kit foams under partial vacuum and that is
another reason I don't use vacuum.
You might have to use vacuum in your case. However, test each EPON kit
component separately to see how much vacuum it can tolerate before foaming.

We have made various sizes of agglomerated spheres of these particles since
the mid 70s as I recall. Anyway, they can be fragile but epon does not
break up the microstructure. One can section throgh the 'micro-dust' on
the surface of these spheres without loss of material.

As you can see, EPON works on a wide range of 'porous silica' samples.
There must be a reason why EM people like it? It works on a wide range of
materials, wets well, sticks fairly well to them, and sections well.

Eponate 12 releases from polyethelene molds, bottle caps, etc. quite well
for 1-4 cycles of mold use with EPON, FYI.

Hope this helps.

Paul Beauregard
Senior Research Associate
PPG Industries
Monroeville Technical Center
Monroeville, PA 15146

Opinions given are my own and not those of PPG Industries.


} Dear all
}
} I have a sample of dried porous silica that I would like to embed and
} thin section. Could anyone give me any advice on the best resin and
} protocol to use? The idea of using a methacrylate resin has been thrown
} about (the idea, not the resin - I can assure you), however I would be
} very grateful for any advice regarding any of the resins available. If
} this is a bit specific then reply off-line to the address below.
}
} Thanks
} Pippa
} ----------------------
} Pippa Hawes, EMU
} School of Chemistry
} University of Bristol
} Cantocks Close
} Bristol UK
} BS8 1TS
} Pippa.Hawes-at-bristol.ac.uk
}
}
}
}



From daemon Sat Sep 1 10:57:01 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 01 Sep 2001 11:53:04 -0500
Subject: YAG and YAP scintillators

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Earl Weltmer and Brad Johnson wrote:
============================================================
} Hello,
} I need to replace the SE scintillator on my SEM (JEOL 5900), and I was
} considering using a YAP scintillator instead of the typical phosphorous
} scintillator. Does anyone have any experience or suggestions on this?
++++Brad Johnson

About ten years ago I ran some tests for signal on several scintillator
types: YAP, YAG, standard aluminum coated scintillator & "uncoated"
scintillator.
The result at that time were surprising.

Using a JEOL 35C SEM & running the same specimen current & PMT voltage with
each scintillator the best signal was given by the original aluminum coated
scintillator.

The second best was the "uncoated" scintillator & it was only 80% of the
signal the standard aluminum coated scintillator.
The YAP & YAG gave only 60% of the standard aluminum scintillator.

I was very disappointed as the YAP & YAG were significantly more than the
standard scintillator.
The conclusion that I came to was that the YAP & YAG would probably give
more longevity & could justify their extra cost but they were not fit for
high resolution. The YAP & YAG would probably be suitable for probe work.

Keep in mind this experiment was done about ten years ago. Perhaps the
crystals have improved in recent years & the data may need a re-evaluation.
I am sure that there is someone who would be willing to criticize my results
(maybe in Northern Calif.?) but this the data that I received at the time.
+++++ Earl Weltmer
============================================================
We have offered P-47, YAG and YAP scintillators for SEM applications for
more then twenty years. Earl is correct that the P-47 performance, **but
when first installed**, does probably give a superior performance. However
, not all YAG and YAP crystals come out of the "same cookie cutter." From
what we have been able to deduce, a high quality YAG or YAP single crystal
scintillator will perform so close to that of the P-47 that one usually has
difficulty detecting the difference. However, as everyone knows, the P-47
once installed, in terms of performance, goes only down. The only question
is how fast it will go down. And that rate of deterioration of performance
depends among other things on the type of work being done. For example, the
higher x-ray fluxes associated with EDS (and especially WDS) tend to cause a
more rapid deterioration.

Also, if Earl's experiment was done as described, and if a YAG was tested
against P-47 but using the same PMT, then one would expect to find an
inferior performance, because the PMT that is optimum for P47 is not the
same one that would have been optimum for YAG. Now this might be less of a
problem today than it would have been ten years ago, but if the PMT was not
changed, then the right test (IMHO) was not the one being performed. To
make the comparison, one would have had to have taken out the S11 style and
replaced it with an S20 PMT. This point is further explained on our website
URL
http://www.2spi.com/catalog/scintill/spi-yag.html

When a YAG or YAP scintillator is installed, its performance level is
constant and does not deteriorate. So while the comparison might suggest
some superiority at the very beginning, in most instances that we have seen,
or have been led to believe, it is not long that the SEM running on YAG or
YAP in general, is operating at a higher level of performance. And the SEM
is not subjected to the downtime associated with the need to change a P-47
powder scintillator from time to time.

For those operating at low KV in the BSE mode (and using YAG or YAP), and
high magnifications, the image is clearly less noisy. A collection of ten
different references from different laboratories around the world, which
have been collected on URL
http://www.2spi.com/catalog/scintill/sem-tem.html
at least to some, pretty much document the superiority of the single crystal
scintillators over the powder scintillators. I guess one could always try
to do more studies, but anyone that is familiar with these publications
finds their conclusions pretty persuasive.

We have generally recommended that the SPI single crystal scintillators
offered many advantages over the more traditional P-47 scintillator. We
have been under the impression that those who have made the switch have been
pretty happy with their results, though we do hear from time to time of
someone who would not agree with that statement. Of course, the performance
is related to the nature of the doping of the crystal, and as I said, not
all these crystals come out of the same cookie cutter. Not all YAG's and
YAP's are created equal. So when making comparisons, and statements about
their scintillators, it would be helpful for one to state the brand of their
scintillator crystal. These crystals are certainly not a generic entity
where all are the same irrespective of the source.

Disclaimer: SPI Supplies is a major supplier of scintillators for SEM
applications including P-47's, YAGs and YAPs.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sat Sep 1 10:58:33 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 1 Sep 2001 08:53:38 -0700
Subject: Re: TEM - embedding silica

Contents Retrieved from Microscopy Listserver Archives
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}
} I have a sample of dried porous silica that I would like to embed and
} thin section. Could anyone give me any advice on the best resin and
} protocol to use? The idea of using a methacrylate resin has been thrown
} about (the idea, not the resin - I can assure you), however I would be
} very grateful for any advice regarding any of the resins available. If
} this is a bit specific then reply off-line to the address below.
}
Pippa -

You'll probably do fine with LR White, Hard grade. Its low viscosity is a
big help, but if the sample floats anyway you may need vacuum. If you've
never used that resin, be cautious when selecting the embedding mold; it
will dissolve some plastics & it won't harden in flat molds unless air is
excluded. Gelatin capsules work.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sat Sep 1 21:00:35 2001



From: Dmrelion-at-aol.com
Date: 09/01/2001
Subject: spectoscopy and spectrometry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Subj: spectrometers and spectroscopes

I have followed this discussion with some interest. A similar situation
exists in the field of mass spectroscopy.

At one time there were many mass spectrographs and mass spectrometers being
used. Mass spectrographs were those instruments in which the detected ions
were spread out along a focal plane and a significant portion of the mass
range was collected simultaneously on a photographic plate. Mass
spectrometers were those instruments in which the detected ions were moved
across a detector slit (by varying magnetic field or ion accelerating
potential) and collected/detected, one m/q at a time, by either a Faraday
cup or electron multiplier after the slit, providing a readout on an
electronic device (electrometer usually). Mass spectroscope or mass
spectroscopy was used as the general term to include either type of
instrument.

Mass spectrographs are rarely seen any more and even if they are used, the
photographic plate would probably be replaced by some type of CCD detector
providing electronic readout so the original clear distinction becomes
blurred.

I'm sure that an analogous situation exists in optical spectroscopy.

Don Marshall



Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Sun Sep 2 00:09:15 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sun, 2 Sep 2001 15:02:52 +1000
Subject: RE: FE-SEM: Historical Question

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"jim-at-proscitech.com"
{jim-at-proscitech.com} ,
"'Angela Klaus'" {avklaus-at-amnh.org} ,
"microscopy-at-sparc5.microscopy.com" {microscopy-at-sparc5.microscopy.com}


Reinhard Rachel also wrote on this back-channel and so I expect that you are
right: I confused the early FE-SEM and FE-TEM.
I saw the Siemens instrument in the Karlsruhe application lab in October 76. I
was there for two other instruments but the large FE instrument was of course
rather eye-catching. 25 years later I recall that it had a short, but large
diameter column and so thought of it in the long-term as a FE-SEM.
Hope that this was not a complete waste of time as these contributions have
placed other FE instruments in a historical context.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, September 01, 2001 6:18 AM, Alan Nicholls [SMTP:nicholls-at-uic.edu]
wrote:
} Jim
}
} I think you mean FE-STEMs not FE-SEMs. The first VG HB5 STEM was delivered
} in 1973 to University of London.
}
} Regards
}
} Alan
}
} At 09:55 AM 8/31/2001 +1000, Jim at Proscitech wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } In 1976 both, Siemens and Vacuum Generator marketed FESEMs. The Vacuum
} } Generator unit was considerably cheaper and possibly better too.
} } Incidentally, the Siemens unit became a fiasco and likely was a major reason
} } for the company's board to abandon the building of electron microscopes a
} } year
} } or two later. Siemens had been the first commercial manufacturer of electron
} } microscopes and built the best instruments in the 50th and early 60th. The
} } Philips EM300 was the first serious challenge to Siemens.
} } Michael Beer of Chicago published a great deal of FESEM developments in the
} } early 70th.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Thursday, August 30, 2001 9:43 PM, Angela Klaus [SMTP:avklaus-at-amnh.org]
} } wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi Listers...
} } }
} } } Does anyone know when the first cold field emission microscope became
} } } commercially available?
} } }
} } } Thanks and best,
} } }
} } } Angela
} } }
} } } Angela V. Klaus
} } }
} } } Director, Interdepartmental Laboratories
} } } American Museum of Natural History
} } } Central Park West at 79th Street
} } } New York, NY 10024 USA
} } }
} } } Email: avklaus-at-amnh.org
} } } Tel: 212-769-5977
} } }
}
} Alan W Nicholls, PhD
} Director of Research Service Facility (Electron Microscopy)
} Research Resources Center - East (M/C 337)
} Room 100 Science and Engineering South Building
} The University of Illinois at Chicago
} 845 West Taylor St
} Chicago, IL 60607-7058
}
} Tel: 312 996 1227
} Fax: 312 996 8091
} Office: Room 110
}
} Web site www.rrc.uic.edu


From daemon Sun Sep 2 02:00:47 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sun, 02 Sep 2001 10:53:41 -0400
Subject: Re: Kudo's to Nestor

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Dear Gary,
I will receive my Axiopklan only on next week - so I have still no
experience and cannot help.
With best wishes
Jiri Kalvoda
----- Original Message -----
} From: Gary Gaugler {gary-at-gaugler.com}
To: {jsharp-at-zeiss.com}
Cc: MSA listserver {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 22, 2001 5:09 AM


Here, here! (or is it hear , hear?)
Ken Converse
Quality Images

Paula Sicurello wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers,
}
} Nestor's little slip and his apology should make us realize how valuable Nestor and his service of the listserver is to us. I just want to say Thank You Nestor for all the hard work you do to keep us on-line and spam free. You help all of us to stay connected in a nice environment where we can ask our questions and communicate our ideas.
}
} Thank You Nestor!
}
}
} Paula :-)
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax
}
}
}
}



From daemon Sun Sep 2 15:28:20 2001



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Sun, 2 Sep 2001 16:19:46 -0400
Subject: NYMS Abbe award to Joseph I. Goldstein

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-----Original Message-----
} From: John A. Reffner [mailto:JAReffner-at-compuserve.com]


On Tuesday, October 2, 2001, Dr. Joseph I. Goldstein will receive the New
York Microscopical Society's Ernst Abbe Memorial Award at a special
symposium in Atlantic City, New Jersey. The Symposium is part of the
Eastern Analytical Symposium & Exposition. The session starts at 9:00-am
with the presentation of the Abbe Award. The program includes:

Joseph I. Goldstein Advances in Scanning Electron Microscopy and
X-Ray
Microanalysis

Charles E. Lyman Quantitative X-Ray Microanalysis in the
Environmental SEM

David B. Williams Microbeam Analysis of Metallic Meteorites

Eric Lifshin Pushing the Limits of Spatial
Resolution in X-Ray
Microanalysis

Come and join the celebration Dean Goldstein's contributions to science of
microscopy and educating so many microscopists.

The EAS meeting will be held in the Atlantic City Convention Center,
October 1-4, 2001. For more information contact the EAS Homepage:(
http://www.eas.org).

John A. Reffner, President NYMS



From daemon Mon Sep 3 05:59:20 2001



From: Divakar :      divakar-at-igcar.ernet.in
Date: Mon, 3 Sep 2001 16:16:35 +0530
Subject: [MatSci] [HRTEM] Semper image file created by EMS

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I have run into a file format problem between Semper for Windows(image
processing software from Synoptics, UK running on Microsoft Windows 98)
and EMS (image simulation software by Prof Stadelmann running on a
Silicon Graphics workstation). The EMS suite of programs for image
simulation has a routine se1 that writes the R type images (created by
the im1 routine, in the present case) in Semper file format which is
essentially a Fortran unformatted output. This file is to be read into
Semper using the unformatted option of the read command. However I get
a

Message: illegal structure for unformatted file
?129: File I/O error 6419 on unit 1 file
'd:\users\divakar\semper\images\rd1082.unf'

error on Semper for Windows and a similar message on Semper for DOS
version 6.4. I would appreciate help / advise from members of this list
in this regard. Is this because the binary code on Unix and DOS
/Windows is different? Or is this a version problem? Does somebody have
software / image file / disc file format details which can be used to
inter-convert between these operating systems and / or hardware?

With Best Regards,
----
Divakar R
Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----






From daemon Mon Sep 3 06:42:45 2001



From: jesper.v.carstensen-at-risoe.dk
Date: Mon, 03 Sep 2001 13:36:55 +0200
Subject: Electroscan E3 SEM question

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Dear Gordon,

We have an Electroscan ESEM E3, on which we have had different problems with
the stage. In one case it was necessary to change some cables to fix the
problem, but occasionally the stage won't move in the X-Y directions, and
the problem is solved simply by changing the magnification on the
microscope!! We haven't yet been able to come up with an explanation for
this strange behaviour, but I hope for you, that your problems may be solved
in this simple way.

Kind regards,
Jesper
________________________________
Jesper Vejloe Carstensen
Electron Microscopy & Microanalysis
Risoe National Laboratory, Materials Research Department
P.O. Box 49, DK-4000 Roskilde, DENMARK
Phone: 46 77 57 76, Fax: 46 77 57 58
E-mail: jesper.v.carstensen-at-risoe.dk
Web: www.risoe.dk/afm




-----Original Message-----
} From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU]
Sent: 31. august 2001 18:24
To: Microscopy-at-sparc5.microscopy.com


Hello,
I've been going through setting up an Electroscan ESEM E3 in our
laboratory and vacuum problems aside, we now have a stage that doesn't
want to move. Anyone ever come across stage problems and have some
suggestions that I may try for this instrument?

The stage will actually move if you give it a gentle push along a gear
arm, but it often locks up in the Z direction, and sometimes X/Y. Auto
calibrate allows the stage to move in X/Y, but it never calibrates the Z
direction.
Thanks.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
\
Gordon Ante Vrdoljak Electron Microscope
Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Mon Sep 3 11:11:35 2001



From: Martin Roe :      Martin.Roe-at-nottingham.ac.uk
Date: Mon, 03 Sep 2001 17:02:31 +0100
Subject: Re: EDS Detector sensitivity problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




Dear fellow list server members,
We have a Si(Li) detector with Be/UT window attached to a SEM. Some time before I started my job here there was a vacuum fault where pump oil backstreamed into the SEM column -my understanding is that the Be window was in position at the time. We are now seeing low counts for light elements when used in thin window mode and quantitative data reflect this, even though the oil streamed in when the Be window was being used. The manufacturer has suggested the likely source is ice on the crystal (from figures below). This problem I am assured only started after the vacuum/oil problem which indicates oil is the likely problem. I include K/L line data collected from a copper sample at 10, 15, 20 kV with both the Be and UT windows in place. The counts represent the peak windows/ROI of the K and L lines.
What do you make of these figures below? As I have no reference data or anything to compare with. I would appreciate any input or suggestions you have.

Beam Window Cu L Cu K K/L ratio
20 Be 47461 156947 3.3
20 utw 84033 110570 1.3
15 Be 18357 24336 1.3
15 utw 34930 18172 0.5
10 Be 15225 1070 0.07
10 utw 28005 880 0.03

Mant thanks.

Martin Roe
Electron Microscopist
Materials/Engineering Department
Wolfson Building
Nottingham University
University Park
Nottingham
NG7 2RD
tel +44 (0) 115 9513768
tel =44 (0) 115 9513871
fax +44 (0) 115 9513764
email: martin.roe-at-nottingham.ac.uk



From daemon Mon Sep 3 21:10:33 2001



From: Paiboon NUANNIN :      npaiboon-at-ratree.psu.ac.th
Date: Tue, 4 Sep 2001 08:59:13 +0700 (ICT)
Subject: Re: EDS Detector sensitivity problem

Contents Retrieved from Microscopy Listserver Archives
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Dear Martin

Due to you didn't mention anything about live time, beam
condition, i.e, beam current, beam alignment, working distance and
ect.. so it is quite difficult to suggest anything about your
results. However, it seems likely that at 15kv (Be) and 20k(utw)
are OK. Normally, at 20kV, K/L ratio is close to 1, it depends on
the condition of the detector.


Try Ni K/L to confirm that, K/L ratio would be about 1 at 20 kV.

Regards,

Paiboob Nuannin
Dept of Physics
Faculty of Science
Prince of Songkla University
Hatyai 90110
Thailand





On Mon, 3 Sep 2001, Martin Roe wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear fellow list server members,
} We have a Si(Li) detector with Be/UT window attached to a SEM. Some time before I started my job here there was a vacuum fault where pump oil backstreamed into the SEM column -my understanding is that the Be window was in position at the time. We are now seeing low counts for light elements when used in thin window mode and quantitative data reflect this, even though the oil streamed in when the Be window was being used. The manufacturer has suggested the likely source is ice on the crystal (from figures below). This problem I am assured only started after the vacuum/oil problem which indicates oil is the likely problem. I include K/L line data collected from a copper sample at 10, 15, 20 kV with both the Be and UT windows in place. The counts represent the peak windows/ROI of the K and L lines.
} What do you make of these figures below? As I have no reference data or anything to compare with. I would appreciate any input or suggestions you have.
}
} Beam Window Cu L Cu K K/L ratio
} 20 Be 47461 156947 3.3
} 20 utw 84033 110570 1.3
} 15 Be 18357 24336 1.3
} 15 utw 34930 18172 0.5
} 10 Be 15225 1070 0.07
} 10 utw 28005 880 0.03
}
} Mant thanks.
}
} Martin Roe
} Electron Microscopist
} Materials/Engineering Department
} Wolfson Building
} Nottingham University
} University Park
} Nottingham
} NG7 2RD
} tel +44 (0) 115 9513768
} tel =44 (0) 115 9513871
} fax +44 (0) 115 9513764
} email: martin.roe-at-nottingham.ac.uk
}
}



From daemon Tue Sep 4 00:37:36 2001



From: marienti-at-tiscalinet.it
Date: Tue, 4 Sep 2001 07:30:38 +0200
Subject: =?iso-8859-1?Q?TEM=20CM10=20Help=21?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers!

This is a request of information about a CM10.

We tried a stretch alignment of the column to verify the basic astigmatism.

In low mag, specially in the central and lower magnification range (between
50 and 250) we see a ?blade? cutting the field of view only without any
condenser aperture inserted.

This blade is the objective aperture support.

I suppose it is a normal thing, but I like to be sure about it.

Is the same on your machine?

If it is so, please let me know.

Thanks a lot!



P.S. Thanks Nestor!



Marco Arienti






__________________________________________________________________
Abbonati a Tiscali!
Con VoceViva puoi anche ascoltare ed inviare email al telefono.
Chiama VoceViva all' 892 800 http://voceviva.tiscali.it






From daemon Tue Sep 4 00:39:21 2001



From: Marco Arienti :      marienti-at-tiscalinet.it
Date: Tue, 4 Sep 2001 07:32:34 +0200
Subject: TEM CM10 Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers!

This is a request of information about a CM10.

We tried a stretch alignment of the column to verify the basic astigmatism.

In low mag, specially in the central and lower magnification range (between
50 and 250) we see a "blade" cutting the field of view only without any
condenser aperture inserted.

This blade is the objective aperture support.

I suppose it is a normal thing, but I like to be sure about it.

Is the same on your machine?

If it is so, please let me know.

Thanks a lot!



P.S. Thanks Nestor!



Marco Arienti







From daemon Tue Sep 4 01:08:19 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 03 Sep 2001 23:05:07 -0700
Subject: Update Zeiss LM Service....

Contents Retrieved from Microscopy Listserver Archives
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Regarding my recent experiences with my used Axioplan, I
consider the issue to be closed. Zeiss sent a factory technician
to my site and corrected the problem at no charge.

gary g.



From daemon Tue Sep 4 02:15:28 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 4 Sep 2001 08:16:15 +0100 (GMT Daylight Time)
Subject: Re: EDS Detector sensitivity problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Martin,

I have little experience with SEM and EDX detector
performance, however, I have used a range of Be, thin
window and windowless detectors on TEMs and there are a
couple of comments I would like to make.

If the degradation in performance occurred directly after
the oil backstreaming then I agree that oil is quite likely
to be the cause but otherwise it is quite possibly ice on
the crystal.

Has the detector liquid nitrogen always been kept well
filled? If the internal 'cryo pump' starts to warm up then
you could get icing on the crystal.

Why not condition the detector anyway? Certainly on my
Oxford Instruments (ex Link) detectors it is easy to do and
does not seem to degrade the performance at all. I
regularly condition the windowless detector on my JEOL2010
TEM (about every 6 months) to get the light element
performace back. However, I have not had to condition the
SATW detector (on another TEM) in over two years so I guess
the water comes from the microscope and not the detector.

Regards,
Ron

On Mon, 03 Sep 2001 17:02:31 +0100 Martin Roe
{Martin.Roe-at-nottingham.ac.uk} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear fellow list server members,
} We have a Si(Li) detector with Be/UT window attached to a SEM. Some time before I started my job here there was a vacuum fault where pump oil backstreamed into the SEM column -my understanding is that the Be window was in position at the time. We are now seeing low counts for light elements when used in thin window mode and quantitative data reflect this, even though the oil streamed in when the Be window was being used. The manufacturer has suggested the likely source is ice on the crystal (from figures below). This problem I am assured only started after the vacuum/oil problem which indicates oil is the likely problem. I include K/L line data collected from a copper sample at 10, 15, 20 kV with both the Be and UT windows in place. The counts represent the peak windows/ROI of the K and L lines.
} What do you make of these figures below? As I have no reference data or anything to compare with. I would appreciate any input or suggestions you have.
}
} Beam Window Cu L Cu K K/L ratio
} 20 Be 47461 156947 3.3
} 20 utw 84033 110570 1.3
} 15 Be 18357 24336 1.3
} 15 utw 34930 18172 0.5
} 10 Be 15225 1070 0.07
} 10 utw 28005 880 0.03
}
} Mant thanks.
}
} Martin Roe
} Electron Microscopist
} Materials/Engineering Department
} Wolfson Building
} Nottingham University
} University Park
} Nottingham
} NG7 2RD
} tel +44 (0) 115 9513768
} tel =44 (0) 115 9513871
} fax +44 (0) 115 9513764
} email: martin.roe-at-nottingham.ac.uk
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Sep 4 06:52:39 2001



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Tue, 04 Sep 2001 08:25:05 -0700
Subject: Re: YAG and YAP scintillators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Gordon-

I'll second what Jesper says. Our E3 also occasionally does the
"lock-up" thing but can always be freed up simply by changing the
magnification. Why this should fix it is, I think, one of the great
mysteries of the universe. We've also occasionally experienced slow movement
of the stage in the XY direction. This can make acquiring a digital image a
real problem at times. Usually you can stop this drift by changing the
pressure in the chamber.....Great Mystery of the Universe #2.....

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.Berkeley.EDU}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 31, 2001 1:23 PM


Charles Garber in his recent posting wrote:
} Not all YAG's and
} YAP's are created equal. So when making comparisons, and statements about
} their scintillators, it would be helpful for one to state the brand of their
} scintillator crystal. These crystals are certainly not a generic entity
} where all are the same irrespective of the source.

I am a bit puzzled by this. I was under the impression that all of the
YAG and YAP scintillators offered for sale in this country originate
from the same ultimate supplier in Europe. Has this changed? Or
perhaps there are different grades of crystal?

Fred Schamber
Aspex LLC


"Garber, Charles A." wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Earl Weltmer and Brad Johnson wrote:
} ============================================================
} } Hello,
} } I need to replace the SE scintillator on my SEM (JEOL 5900), and I was
} } considering using a YAP scintillator instead of the typical phosphorous
} } scintillator. Does anyone have any experience or suggestions on this?
} ++++Brad Johnson
}
} About ten years ago I ran some tests for signal on several scintillator
} types: YAP, YAG, standard aluminum coated scintillator & "uncoated"
} scintillator.
} The result at that time were surprising.
}
} Using a JEOL 35C SEM & running the same specimen current & PMT voltage with
} each scintillator the best signal was given by the original aluminum coated
} scintillator.
}
} The second best was the "uncoated" scintillator & it was only 80% of the
} signal the standard aluminum coated scintillator.
} The YAP & YAG gave only 60% of the standard aluminum scintillator.
}
} I was very disappointed as the YAP & YAG were significantly more than the
} standard scintillator.
} The conclusion that I came to was that the YAP & YAG would probably give
} more longevity & could justify their extra cost but they were not fit for
} high resolution. The YAP & YAG would probably be suitable for probe work.
}
} Keep in mind this experiment was done about ten years ago. Perhaps the
} crystals have improved in recent years & the data may need a re-evaluation.
} I am sure that there is someone who would be willing to criticize my results
} (maybe in Northern Calif.?) but this the data that I received at the time.
} +++++ Earl Weltmer
} ============================================================
} We have offered P-47, YAG and YAP scintillators for SEM applications for
} more then twenty years. Earl is correct that the P-47 performance, **but
} when first installed**, does probably give a superior performance. However
} , not all YAG and YAP crystals come out of the "same cookie cutter." From
} what we have been able to deduce, a high quality YAG or YAP single crystal
} scintillator will perform so close to that of the P-47 that one usually has
} difficulty detecting the difference. However, as everyone knows, the P-47
} once installed, in terms of performance, goes only down. The only question
} is how fast it will go down. And that rate of deterioration of performance
} depends among other things on the type of work being done. For example, the
} higher x-ray fluxes associated with EDS (and especially WDS) tend to cause a
} more rapid deterioration.
}
} Also, if Earl's experiment was done as described, and if a YAG was tested
} against P-47 but using the same PMT, then one would expect to find an
} inferior performance, because the PMT that is optimum for P47 is not the
} same one that would have been optimum for YAG. Now this might be less of a
} problem today than it would have been ten years ago, but if the PMT was not
} changed, then the right test (IMHO) was not the one being performed. To
} make the comparison, one would have had to have taken out the S11 style and
} replaced it with an S20 PMT. This point is further explained on our website
} URL
} http://www.2spi.com/catalog/scintill/spi-yag.html
}
} When a YAG or YAP scintillator is installed, its performance level is
} constant and does not deteriorate. So while the comparison might suggest
} some superiority at the very beginning, in most instances that we have seen,
} or have been led to believe, it is not long that the SEM running on YAG or
} YAP in general, is operating at a higher level of performance. And the SEM
} is not subjected to the downtime associated with the need to change a P-47
} powder scintillator from time to time.
}
} For those operating at low KV in the BSE mode (and using YAG or YAP), and
} high magnifications, the image is clearly less noisy. A collection of ten
} different references from different laboratories around the world, which
} have been collected on URL
} http://www.2spi.com/catalog/scintill/sem-tem.html
} at least to some, pretty much document the superiority of the single crystal
} scintillators over the powder scintillators. I guess one could always try
} to do more studies, but anyone that is familiar with these publications
} finds their conclusions pretty persuasive.
}
} We have generally recommended that the SPI single crystal scintillators
} offered many advantages over the more traditional P-47 scintillator. We
} have been under the impression that those who have made the switch have been
} pretty happy with their results, though we do hear from time to time of
} someone who would not agree with that statement. Of course, the performance
} is related to the nature of the doping of the crystal, and as I said, not
} all these crystals come out of the same cookie cutter. Not all YAG's and
} YAP's are created equal. So when making comparisons, and statements about
} their scintillators, it would be helpful for one to state the brand of their
} scintillator crystal. These crystals are certainly not a generic entity
} where all are the same irrespective of the source.
}
} Disclaimer: SPI Supplies is a major supplier of scintillators for SEM
} applications including P-47's, YAGs and YAPs.
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================


From daemon Tue Sep 4 08:45:16 2001



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Tue, 04 Sep 2001 08:45:20 -0700
Subject: RE: FE-SEM: Historical Question

Contents Retrieved from Microscopy Listserver Archives
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Jim

I think you mean FE-STEMs not FE-SEMs.

The first VG HB5 FE-STEM was delivered in 1973 to University of London.

Both AEI and Seimens got into dedicated STEM shortly before they pulled out
of the EM market in the early 70's. VG was able to attract a number of the
main characters who had worked at AEI to form the core of the VG
Microscopes unit.

The first electron microscope VG produced was a W thermal sourced SEM
(Miniscan) for which they were awarded the Queens award for technological
innovation in 1970. They then went on to produce the HB5 High Vacuum
FE-STEM and HB50 High Vacuum FE-SEM. All of the latter were sold with
Auger spectrometers as a Scanning Auger Microprobe.

Regards

Alan

At 09:55 AM 8/31/2001 +1000, Jim at Proscitech wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Tue Sep 4 09:38:54 2001



From: Bruce Ingber :      bingber-at-srrc.ars.usda.gov
Date: Tue, 04 Sep 2001 09:31:04 -0500
Subject: Electroscan E3 SEM question

Contents Retrieved from Microscopy Listserver Archives
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Dear Bruce

This is normal!

On both the E3 and 2020 the stage movement with the Joy Stick is linked to
magnification. The higher the mag the slower the stage speed. Above about
8000 X the stage motors are disabled and beam shift is active with the Joy
Stick.

The apparent movement of the image should remain the same at all mags. If
the mag is above about 8KX then the amount of image movement is limited by
the beam shift. To continue searching the magnification has to be lowered.

For ElectroScan ESEM questions (E2,E3,Explorer, 2010 or 2020) world wide
contact me directly at dsimpson-at-ma.feico.com
For XL ESEM (XLFEG ESEM, XLLab6 ESEM or XLTMP ESEM in North America contact
me directly at dsimpson-at-ma.feico.com
For XL ESEM (XLFEG ESEM, XLLab6 ESEM or XLTMP ESEM for the rest of the world
contact their local Service group.


Regards
Derek Simpson
ESEM Technical Support Manager
FEI Company
One Corporation Way #2
Peabody, MA 01960
E-mail: dsimpson-at-feico.com
Voice: 1.978.538.6700
Fax: 1.978.531.9648


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Gordon,

We have an Electroscan ESEM E3, on which we have had different problems with
the stage. In one case it was necessary to change some cables to fix the
problem, but occasionally the stage won't move in the X-Y directions, and
the problem is solved simply by changing the magnification on the
microscope!! We haven't yet been able to come up with an explanation for
this strange behaviour, but I hope for you, that your problems may be solved
in this simple way.

Kind regards,
Jesper
________________________________
Jesper Vejloe Carstensen
Electron Microscopy & Microanalysis
Risoe National Laboratory, Materials Research Department
P.O. Box 49, DK-4000 Roskilde, DENMARK
Phone: 46 77 57 76, Fax: 46 77 57 58
E-mail: jesper.v.carstensen-at-risoe.dk
Web: www.risoe.dk/afm


-----Original Message-----
} From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU]
Sent: 31. August 2001 18:24
To: Microscopy-at-sparc5.microscopy.com


Hello,
I've been going through setting up an Electroscan ESEM E3 in our
laboratory and vacuum problems aside, we now have a stage that doesn't
want to move. Anyone ever come across stage problems and have some
suggestions that I may try for this instrument?

The stage will actually move if you give it a gentle push along a gear
arm, but it often locks up in the Z direction, and sometimes X/Y. Auto
calibrate allows the stage to move in X/Y, but it never calibrates the Z
direction.
Thanks.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
\
Gordon Ante Vrdoljak Electron Microscope
Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793




From daemon Tue Sep 4 10:36:10 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Tue, 4 Sep 2001 11:30:30 -0400
Subject: Bench Top Turbo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Krassimer,

An alternative you may want to consider is the Polaron E6700 Evaporator,
manufactured by Thermo VG Scientific. We are their distribution and service
agent in the U.S. and would be happy to supply you with more information.

Best regards,

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
mnesta-at-ebsciences.com
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: K.N. Bozhilov [mailto:bozhilov-at-citrus.ucr.edu]
Sent: Friday, August 31, 2001 12:27 PM
To: microscopy-at-sparc5.microscopy.com


Hi All,

I would greatly appreciate if you could share your opinion about performance
and reliability of the Bench Top Turbo III vacuum evaporator from Denton
Vacuum, also alternative suggestions are more than welcome. We are looking
currently to purchase a vacuum evaporator for general EM preparation capable
of carbon and metal evaporation, glow discharge and thickness control.
Cleanliness of the vacuum is our main concern and especially the fact that
the Bench Top model does not have LN trap.

Thank you,

Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel 909 787 2998
Fax 909 787 4324





From daemon Tue Sep 4 11:31:04 2001



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Tue, 4 Sep 2001 12:24:16 -0400
Subject: Oil diffusion pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


List,

We are parting with a used oil diffusion pump (w/ LN2 Trap) that has approx.
2 years wear and tear. The pump has been in storage for the last 10 years.
The pump was manufactured by DAIA vacuum Engineering corp, model # DPF4Zs.
The mounting flange is approx. 7.25 inches with an 8 hole bolt pattern. It
was removed from a Hitachi S-4000 FESEM for replacement by a cleaner turbo
model. If you are interested please contact me off-line. The pump is free
but you must assume all shipping costs. Thanks, jr

Particulars: 570 l/sec
100V / 500W
Pressure = 10 -7torr
Oil capacity 150cc


John A. Robson
} _____________________________________________________
}
} Boehringer Ingelheim Pharmaceuticals, Inc.
} Research and Development
} 900 Ridgebury Road / P. O. Box 368
} Ridgefield, CT 06877-0368
}
} phone: 203.798.5640
} fax: 203.798.5698
} email: jrobson-at-rdg.boehringer-ingelheim.com
}
}
}


From daemon Tue Sep 4 11:36:02 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 04 Sep 2001 11:30:35 -0500
Subject: Re: [MatSci] [HRTEM] Semper image file created by EMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I will hazard one guess being unfamiliar with either of the programs or
formats that you describe. I suspect that there is a difference in how the
two systems format text files. Under DOS and Windows, text lines normally
end with a carriage return and line feed characters, ASCII codes 13 and 10.
Under UNIX, lines often end with one or the other, but not both; I think
carriage return is the norm.

I know that WS-FTP has an option for converting text files to the proper
standard when transferring between the two different platforms. It might be
able to help, but you will need to tell it that the file are text. It
ordinarily runs in auto-detect mode and determines file type based on the
extension. Unknown extensions default to binary format. Presuming you have
to ship your files anyway, this might eliminate the need for a translator
program.

There are also a number of utilities around that could give you a look at
the binary codes for your file to see what characters are being used to
indicate the end of the line. I have some I could recommend on the PC side
if you don't already have one. They are relatively available on the
shareware sites and could help verify what I have said. Some even serve as
editors so you could make changes to one file manually to see if this is
the source of your problem.

Warren

At 04:16 PM 9/3/2001 +0530, you wrote:

} I have run into a file format problem between Semper for Windows(image
} processing software from Synoptics, UK running on Microsoft Windows 98)
} and EMS (image simulation software by Prof Stadelmann running on a
} Silicon Graphics workstation). The EMS suite of programs for image
} simulation has a routine se1 that writes the R type images (created by
} the im1 routine, in the present case) in Semper file format which is
} essentially a Fortran unformatted output. This file is to be read into
} Semper using the unformatted option of the read command. However I get
} a
}
} Message: illegal structure for unformatted file
} ?129: File I/O error 6419 on unit 1 file
} 'd:\users\divakar\semper\images\rd1082.unf'
}
} error on Semper for Windows and a similar message on Semper for DOS
} version 6.4. I would appreciate help / advise from members of this list
} in this regard. Is this because the binary code on Unix and DOS
} /Windows is different? Or is this a version problem? Does somebody have
} software / image file / disc file format details which can be used to
} inter-convert between these operating systems and / or hardware?
}
} With Best Regards,
} ----
} Divakar R
} Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
} Kalpakkam 603102, India
} ----

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Tue Sep 4 12:00:14 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Tue, 04 Sep 2001 12:54:41 -0400
Subject: Re: Bench Top Turbo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Krassimir,

It is Ladd's belief that the ideal vacuum evaporator should include a
mechanical and a diffusion pump to get you to the 10(-7) range and
should have a LN trap. The evaporation system should have a protective
shield for cleanliness.
If you are doing chromium evaporation then a turbo pump would be
required.

John Arnott

Disclaimer: Ladd Research manufactures and sells vacuum evaporation
systems.
--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
FAX: 1-802-660-8859
e-mail: sales-at-laddresearch.com

Quality Since 1955


K.N. Bozhilov wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Hi All,
}
} I would greatly appreciate if you could share your opinion about performance
} and reliability of the Bench Top Turbo III vacuum evaporator from Denton
} Vacuum, also alternative suggestions are more than welcome. We are looking
} currently to purchase a vacuum evaporator for general EM preparation capable
} of carbon and metal evaporation, glow discharge and thickness control.
} Cleanliness of the vacuum is our main concern and especially the fact that
} the Bench Top model does not have LN trap.
}
} Thank you,
}
} Krassimir N. Bozhilov
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} Tel 909 787 2998
} Fax 909 787 4324


From daemon Tue Sep 4 12:00:19 2001



From: Joyce Craig :      bafpjec-at-csu.edu
Date: Tue, 04 Sep 2001 11:55:20 -0500
Subject: knifebreaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


It is possible to break the 8mm glass, but it can be very frustrating.
If the users want wider knives, try to talk them into purchasing a
diamond knife for thick sections. It is less expensive than diamonds
for thin sectioning, and I think it is a good buy because of time saved
making and changing knives and attaching water catchers.
The only downside is that once there is a nick in the edge it is there
until resharpening time.



From daemon Tue Sep 4 12:58:01 2001



From: =?iso-8859-1?Q?Patr=EDcia?= Reis :      pmreis-at-morango.esb.ucp.pt
Date: Tue, 4 Sep 2001 12:52:58 -0500
Subject: buffer preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi everyone,

I would like to prepare 0.05 M Cacodylate buffer pH 7.4, but do not
have the recipe and protocol necessary for its preparation.
Would it be possible to help me out?

Thanks in advance.

Patrícia Reis
____________________________
Escola Superior Biotecnologia
Universidade Católica Portuguesa
Rua Dr. António Bernardino Almeida
4200-072 Porto
PORTUGAL
Tel.: 225580044
Fax.: 225090351
e.mail: {mailto:pmreis-at-mail.esb.ucp.pt} pmreis-at-mail.esb.ucp.pt


From daemon Tue Sep 4 13:42:26 2001



From: Judy Trogadis :      TrogadisJ-at-smh.toronto.on.ca
Date: Tue, 04 Sep 2001 09:13:22 -0400
Subject: imaging courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists:

I would like to attend a course on microscopy and imaging techniques which would include the latest developments in this field. Apart from the ones at MBL, Cold Spring Harbor and Jim Pawley's course in June, does anyone know of others. Being quite keen, I would prefer something this winter but any suggestions would be highly appreciated.

Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Tue Sep 4 14:49:06 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Tue, 04 Sep 2001 12:40:36 -0700
Subject: Minot Microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
A colleague recently inherited an IEC Minot Rotary Microtome. Does anyone
have a manual or parts? It is missing the speciman holder, mounting bar
for knife height adjustment and means of measuring knife angle.

Thanks in advance,
Glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************




From daemon Tue Sep 4 18:56:39 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 4 Sep 2001 17:36:41 -0600
Subject: Re: [MatSci] [HRTEM] Semper image file created by EMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Could also be a processor related problem. There is a difference in the way
the Intel and Motorola processors store information. One of the two writes
the LSB (least significant byte) first, the other the MSB (most significant
byte). The best bet would probably be to use a format that can be read by
both. TIF, for example, can be normally read by both as the file itself
contains information about the byte order.

Can you transform your images into TIF?

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Warren E Straszheim [mailto:wesaia-at-iastate.edu]
Sent: Tuesday, September 04, 2001 10:31 AM
To: divakar-at-igcar.ernet.in
Cc: Microscopy-at-sparc5.microscopy.com


I will hazard one guess being unfamiliar with either of the programs or
formats that you describe. I suspect that there is a difference in how the
two systems format text files. Under DOS and Windows, text lines normally
end with a carriage return and line feed characters, ASCII codes 13 and 10.
Under UNIX, lines often end with one or the other, but not both; I think
carriage return is the norm.

I know that WS-FTP has an option for converting text files to the proper
standard when transferring between the two different platforms. It might be
able to help, but you will need to tell it that the file are text. It
ordinarily runs in auto-detect mode and determines file type based on the
extension. Unknown extensions default to binary format. Presuming you have
to ship your files anyway, this might eliminate the need for a translator
program.

There are also a number of utilities around that could give you a look at
the binary codes for your file to see what characters are being used to
indicate the end of the line. I have some I could recommend on the PC side
if you don't already have one. They are relatively available on the
shareware sites and could help verify what I have said. Some even serve as
editors so you could make changes to one file manually to see if this is
the source of your problem.

Warren

At 04:16 PM 9/3/2001 +0530, you wrote:

} I have run into a file format problem between Semper for Windows(image
} processing software from Synoptics, UK running on Microsoft Windows 98)
} and EMS (image simulation software by Prof Stadelmann running on a
} Silicon Graphics workstation). The EMS suite of programs for image
} simulation has a routine se1 that writes the R type images (created by
} the im1 routine, in the present case) in Semper file format which is
} essentially a Fortran unformatted output. This file is to be read into
} Semper using the unformatted option of the read command. However I get
} a
}
} Message: illegal structure for unformatted file
} ?129: File I/O error 6419 on unit 1 file
} 'd:\users\divakar\semper\images\rd1082.unf'
}
} error on Semper for Windows and a similar message on Semper for DOS
} version 6.4. I would appreciate help / advise from members of this list
} in this regard. Is this because the binary code on Unix and DOS
} /Windows is different? Or is this a version problem? Does somebody have
} software / image file / disc file format details which can be used to
} inter-convert between these operating systems and / or hardware?
}
} With Best Regards,
} ----
} Divakar R
} Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
} Kalpakkam 603102, India
} ----

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of
materials
Computer applications and networking



From daemon Tue Sep 4 19:46:29 2001



From: Long Miao :      lmiao-at-bio.fsu.edu
Date: Tue, 04 Sep 2001 20:41:52 -0400
Subject: How to refill the micromanipulator system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear lister?
Our Narishige WR-90 micromanipulator does not work properly because the
the water inside the system has leaked out slowly. Does anyone in the list
know how to refill it? Any suggestion is highly appreciated.
Thanks,
Long
-----------------------------------
Miao, Long
Dept of Biological Science
334 Bio. Unit1
Florida State University
Tallahassee, FL32306-4370
email: lmiao-at-bio.fsu.edu
Voice: (850)644-9817
FAX : (850)644-0481

-----------------------------------



From daemon Tue Sep 4 20:28:24 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 4 Sep 2001 20:23:07 -0500
Subject: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
If any of you have removed a jammed film cassette from a JEOL
1200EXII, I
would like to know how you dislodged it. We will eventually have a service
technician to help but I cannot figure out how to remove the plate. I did
manage to remove the film and yes the plate was inserted right side up.
Thanks in advance.
Rosemary


From daemon Tue Sep 4 22:49:37 2001



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Tue, 4 Sep 2001 23:42:32 -0400
Subject: imaging courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


A one day short course on "Digital Image Capture and Management in Light
Microscopy" by Dr. Mary McCann & Dr. John McCann is being offered on Sunday
September 30,2001 at the Eastern Analytical Symposium and Exhbition in
Atlantic City, NJ.

Don O'Leary

-----Original Message-----
} From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca]
Sent: Tuesday, September 04, 2001 9:13 AM
To: Microscopy-at-sparc5.microscopy.com
Cc: Judy Trogadis


Fellow Microscopists:

I would like to attend a course on microscopy and imaging techniques which
would include the latest developments in this field. Apart from the ones at
MBL, Cold Spring Harbor and Jim Pawley's course in June, does anyone know of
others. Being quite keen, I would prefer something this winter but any
suggestions would be highly appreciated.

Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca






From daemon Wed Sep 5 02:42:47 2001



From: :      mcmouldk-at-203.193.14.120
Date: Wed, 5 Sep 2001 15:33:33 +0800
Subject: JEOL Camera Jams

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Rosemary,

I have experience many jams. Generally occurring with old age. Anyway,
JEOL camera mechanisms are wonderful mechanical
beasts. Two pushing nails in the mechanism catch the plates. If you look
at the plates, on one side there are two small
rectangles, which are cut out. The nails use these to capture the plates
and push them through the mechanism. These
nails, together with the plates wear down and eventually sometimes they
miss and do not mate. Generally jamming in the
open slot where the plates come out of the box.

So, to dislodge a jam there are several solutions:

1) Waggle the mechanisms in the hope it clears - not a good choice.
2) Make a piece of stiff plastic sheet the width of a plate and the length
around 15". Try to insert the sheet above
the canisters but below the top mechanisms so as to lift the nails free of
the canisters. You can see the nails when you
look into the camera above the boxes on both sides. They look like small
arms with a piece of copper alloy on the end.
Pull camera out if nails are free.
3) Remove the whole camera mechanism. Simply remove the bolts from
below the camera chamber (6 I think), and then pull
the camera mechanism out in one piece. The jam can then be freed by
rotating the mechanism a wee bit.

If you have frequent jams, consider replacing the pushing nails (first) then the
plates. Also a good idea to replace
the Teflon sheet bearings, but get a JEOL engineer to do this otherwise the
position of the mechanism could be lost.

Good luck,

Keith.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. K. Moulding.

Materials Characterisation and Preparation Facility
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Wed Sep 5 06:26:35 2001



From: Alan Davis :      adavis-at-saipan.com
Date: Wed, 5 Sep 2001 21:16:23 +1000
Subject: Camera Lucida drawing tube (Zeiss) questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have gotten ahold of a Zeiss camera lucida drawing tube. It has a mirror at an angle that allows one to draw underneath the inclined eye tube. Works for a Zeiss Standard 16. I am getting the hang of it a bit. However, I have at least one question:

What is the collar for (referred to as "ring clamp" below)? It is somewhat eccentric, but I cannot imagine any purpose for it. The device doesn't fit over it, but nearly does. It *is* useful for attaching it to a wider eyepiece tube.

This plastic collar fits snugly inside the eyepiece tube of an Olympus SZH; this is good. Also, it fits over the end of the standard 16 eye tube, with a barrier that doesn't allow it to slip down over the tube. i have some documentation for a similar drawing tube, but there is a slight difference in the picture (there is a knurled knob at the point where the mirror/prism fits over the eyepiece. Mine doesn't have that, it fits snug). Here is the relevant section from the documentation for the pictured device:

1. Focus microscope.

2. Remove eyepiece and slip ring clamp on to tube. Replace eyepiece
and fix with ring clamp. [I referred to the "ring clamp" as a collar.]

3. Clamp drawing prism on the eyepiece. [I assume this refers to the knurled
screw that isn't present on my device---mine doesn't need to be clamped on.]



} From reading the description, though, I don't know what it is for. Is it a spacer to move the eyepiece up higher? Which Zeiss is it really designed for?

Also, I would greatly appreciate any tips. The tips I found in MICSCAPE were helpful. Can I initiate some correspondence with someone who has some experience with one of these fantastic devices?

Alan Davis
Marianas High School

--
adavis-at-saipan.com 1-670-235-6580
Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI

I have steadily endeavored to keep my mind free, so as to give up any
hypothesis, however much beloved -- and I cannot resist forming one
on every subject -- as soon as facts are shown to be opposed to it.
-- Charles Darwin (1809-1882)






From daemon Wed Sep 5 07:33:24 2001



From: JHumenansky-at-phi.com
Date: Wed, 5 Sep 2001 07:16:26 -0500
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Rosemary, I believe that the JEOL 1200 camera chamber is the same as the
JEOL 100CX. You can slide out the film tray by making two metal strips
measuring about 1" to 2" wide and about 10" to 12" long. Bend one end of
each strip in the shape of an allen wrench to use as a sort of handle.
These metal strips are used to raise up the two little arms that are
locking up the film tray. While sitting on the floor so that the open
camera chamber is at eye level and using a bright light you can look into
the top area of the camera chamber just above where the film boxes are
located. On each side and above the film boxes you will see these arms
sticking down. You can use the metal strips to release these arms be
sliding the metal strips straight in.
Be sure that the film tray is all the way in so that there is no force on
these arms holding them down. They are spring loaded and should raise up
with only gently pushing on the metal strips. Be careful and do not apply
a lot of force. You should be able to see these arms raise when you push
in the metal strip. When both arms are raised the drawer should pull out
easily.
Again, don't use force. Hope that this helps.

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387



From daemon Wed Sep 5 08:15:36 2001



From: Patton Steve T Contr AFRL/MLBT :      Steve.Patton-at-wpafb.af.mil
Date: Wed, 5 Sep 2001 09:08:55 -0400
Subject: position opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


To all. Note the following opportunity.

Steve Patton

Systran Federal Corporation in Dayton, Ohio, is managing an "on-site" Contract for the Materials and Manufacturing Directorate (ML), Air Force Research Laboratory (AFRL), at Wright Patterson Air Force Base, in Dayton, Ohio. Candidates are sought for a research position at AFRL/ML, and this position entails research and development on understanding surface forces and surface chemistry of MEMS devices and MEMS materials:

a) study surface forces of MEMS using scanning probe microscopes and special adhesion/friction testers as a function of environment (humidity, temperature, vacuum); develop an understanding of how surface forces, and hence friction and wear may be controlled using surface treatments including monolayers and solid coatings
b) study surface chemistry, tribochemistry, and coating microstructure to elucidate the mechanisms controlling friction and wear in MEMS systems under extreme environments
c) research lubricant transport behavior and dynamics in MEMS systems.

The successful candidate will have knowledge/experience of MEMS devices, surface chemistry, surface forces and tribology. Expertise in surface chemical probes such as scanning Auger microscopy, X-ray photoelectron spectroscopy, FTIR microscopy / grazing angle FTIR, micro Raman, and SEM/EDS are highly desirable. The candidate will use apparatus to study surface forces at the nano scale (scanning probe microscopes, special adhesion testers, and nano/micro friction measurement devices). Working knowledge of monolayer and solid coatings is also highly desirable. The focus of the research effort is to understand the chemistry and physics controlling friction and wear and to use that understanding to develop effective technologies leading to reliable MEMS operation in extreme environments. Analytical instrumentation is currently available, but the successful candidate must be capable of modifying instrumentation as part of the research program.

The advertised position is a full-time job with complete benefits (paid vacation, health and dental coverage, 401K, etc.). Interested candidates should respond to this advertisement by sending their resumes along with a listing of technical publications to the contact person listed below.

Dr. V. ("Nagu") Nagarajan
V.P. & Program Manager
Systran Federal Corporation
4027 Colonel Glenn Highway, Suite 210
Dayton, OH 45431-1672

Tel: (937)-429-9008, ext. 353
Fax: (937)-429-9461

E-mail: nagu-at-systranfederal.com
Web: http://www.systranfederal.com




From daemon Wed Sep 5 09:26:06 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Wed, 5 Sep 2001 09:16:48 -0500
Subject: carbon sticky tab arrays

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Dear Listers,

We have an SEM application which requires an array of samples to be loaded
into the microscope. For producing the array, we need to affix sticky tabs
to an aluminum block (48 tabs in a 6 x 8 arrangement on a block about 3
inches by 4 inches).

Presently, we are producing these arrays by hand, but it would be much more
efficient to produce the arrays in this form by simply pressing a sheet onto
the block, then peel it off to give us all of the 48 tabs properly located
on the plate in one shot.

Does anyone have a name of the manufacturer(s) of carbon sticky tabs, so we
can contact them about this. Our supplier is not willing to provide this
information.

Thank You,

Wharton Sinkler
UOP LLC
Des Plaines, IL



From daemon Wed Sep 5 12:14:20 2001



From: Axel Blau :      axel-at-caltech.edu
Date: Wed, 5 Sep 2001 10:03:28 -0700
Subject: LOMO water-immersion/dipping lenses for 160 mm-corrected scopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On our search for high N.A. water-dipping/immersion objectives for 160
mm-corrected microscopes we came across some lenses made by a Russian
company called 'LOMO Optics' (www.lomooptics.com), which we tried out on our
Nikon Optiphot 2 two-photon scope, and on a Nikon Measurescope MM-11 (bottom
illumination). Their performance is quite astonishing. And since we had to
look for quite a while to find such lenses, we wanted to let the rest of the
community know that you do not have to give away your old 160 mm-corrected
scope yet. Prices are quite reasonable too. At LOMO they are willing to let
you try out the lenses for yourself. But do not be surprised to get a phone
call every day during that time. (And do not forget to negotiate!)

[1] Type EAF-A30-1, TT079, 30x water-immersion, N.A. 0.9, w.d. 1.3 mm, 0.17
mm coverslip correction (performance does not seem to suffer without
coverslip), built-in iris, outer diameter: 0.545" at the tip for about 0.2",
and 0.825" thereafter (the objective has a cap of that diameter, which (at
least in our case) could be removed, giving an even smaller outer diameter
of 0.710"), overall length when installed: ~1.050".

[2] Type 96001, 70x water-dipping (apparently no coverslip correction).,
N.A. 1.23, w.d. 220 µm, outer diameter -at- the tip: 0.426" to 0.585" conical
for about 0.2", 0.800" above,overall length when installed: 1.26".

[3] Type 83127, 85x water-immersion., N.A. 1.0, w.d. between 200 and 230 µm
(depending on the coverslip correction setting, which ranges from 100 µm to
200 µm), outer diameter -at- the tip: 0.733" for about 0.2", 0.825" above,
overall length when installed: 1.26".

[4] Apparently they also sell a 100x water-immersion lens by now, which we
have not seen yet.

With best regards,

Axel

__________________________________

Axel Blau
California Institute of Technology
Division of Biology 156-29
1200 E. California Blvd.
Pasadena, CA 91125

(626) 395-6787 phone
(626) 564-8709 fax

http://wecoyote.caltech.edu/~axelb/
http://www.caltech.edu/~pinelab/pinelab.html



From daemon Wed Sep 5 13:00:23 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 5 Sep 2001 13:50:41 -0400
Subject: carbon sticky tab arrays

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I was at a talk where the carbon sticky dots were first presented. I wish I could remember who was speaking. I know that SPI has the exclusive distribution rights to them. I believe that they were developed by a group at Dupont. I would check the literature and see if they published. Try back issues of the meeting abstracts. I think that the talk that I went to was about 10 years ago.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Wednesday, September 05, 2001 10:17 AM
To: 'Microscopy-at-sparc5.microscopy.com'
Cc: Kuechl, Dorothy



Dear Listers,

We have an SEM application which requires an array of samples to be loaded
into the microscope. For producing the array, we need to affix sticky tabs
to an aluminum block (48 tabs in a 6 x 8 arrangement on a block about 3
inches by 4 inches).

Presently, we are producing these arrays by hand, but it would be much more
efficient to produce the arrays in this form by simply pressing a sheet onto
the block, then peel it off to give us all of the 48 tabs properly located
on the plate in one shot.

Does anyone have a name of the manufacturer(s) of carbon sticky tabs, so we
can contact them about this. Our supplier is not willing to provide this
information.

Thank You,

Wharton Sinkler
UOP LLC
Des Plaines, IL



From daemon Wed Sep 5 14:02:17 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 5 Sep 2001 14:44:36 -0400 (EDT)
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I can't help you get it out, but just wanted to warn you that it might be
possible for you to get your fingers crushed or severed if you're fishing
around in the chamber and the jam suddenly frees itself. BE CAREFUL.
I'm not sure if this is the case with your model, but it was on an older
JEOL that we had. I'd wait for the tech or at least get his advice
before reaching in.

We had some that would get caught, and the only thing to do with them
after they were freed was to toss them. Don't try to straighten them.

Sara

On Tue, 4 Sep 2001, Rosemary Walsh wrote:

} Date: Tue, 4 Sep 2001 20:23:07 -0500
} From: Rosemary Walsh {rw9-at-psu.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: removing jammed TEM film cassettes
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} If any of you have removed a jammed film cassette from a JEOL
} 1200EXII, I
} would like to know how you dislodged it. We will eventually have a service
} technician to help but I cannot figure out how to remove the plate. I did
} manage to remove the film and yes the plate was inserted right side up.
} Thanks in advance.
} Rosemary
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Sep 5 14:22:09 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 5 Sep 2001 15:22:45 -0400
Subject: RE: Plate Jams

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The jamming of the plate drive mechanisms in electron microscopes is
nothing new. I remember back in the 1960s when I was using our two
JEM-6A TEMs in my classes on electron Microscopy. With an average
enrollment of 25 to 30 students per term, coming from all disciplins
across campus, and having all degrees of mechanical aptitude, plate
jams were not an uncommon event. The easiest way to clear up a really
bad plate jam in the JEM-6As was to reach inside the plate chamber
and wiggle the plate carrier around a bit until things worked loose.
Unfortunately, however, the chamber opening was too small for my
hands to get in deep enough to take care of a bad jam, and so on
several occasions I had to enlist the help of my 5-year-old son, who
eventually became quite expert at taking care of the problem. Times
do change, but digital cameras have their problems too!

Good luck, and keep smiling,
WCB
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Wed Sep 5 14:48:27 2001



From: Paul.Nolan-at-alcan.com
Date: Wed, 5 Sep 2001 14:42:43 -0500
Subject: Vibrating Engraver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know where I could purchase a battery powered vibrating
engraving tool?
We have the corded type but I need a portable one to use out on a factory
floor.
Vendor replies welcome


Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com


From daemon Wed Sep 5 14:48:28 2001



From: =?iso-8859-1?Q?Patr=EDcia?= Reis :      pmreis-at-morango.esb.ucp.pt
Date: Wed, 5 Sep 2001 14:43:48 -0500
Subject: about working with fixatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all
I would like an advice. Can anyone suggest a better type of dishes to
use with glutheraldehyde and/or osmium tetroxide during fixation
time. Which bottles are better to store these fixatives?
Many thanks.

Patricia
____________________________
Patrícia João Reis (PhD student)
Escola Superior Biotecnologia
Universidade Católica Portuguesa
Rua Dr. António Bernardino Almeida
4200-072 Porto
PORTUGAL
Tel.: 225580044
Fax.: 225090351
e.mail: {mailto:pmreis-at-mail.esb.ucp.pt} pmreis-at-mail.esb.ucp.pt


From daemon Wed Sep 5 15:33:10 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Wed, 5 Sep 2001 16:24:05 -0400
Subject: Bio. Fixation: What is Champy's Fluid?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




"Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed by
ETOH dehydration, CPD, mounting and SEM viewing.


O.k., we give up, searched numberous books, papers, catalogs, web
sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
"Champy's Solution"? Please?

Thank you much!

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Wed Sep 5 15:52:17 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Wed, 5 Sep 2001 16:50:49 -0400
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Are you referring to the fingers?


} We had some that would get caught, and the only thing to do with them
} after they were freed was to toss them. Don't try to straighten them.
}

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Wed Sep 5 17:38:32 2001



From: Neelima Shah :      shahn-at-mail.med.upenn.edu
Date: Wed, 05 Sep 2001 18:38:24 -0400
Subject: TEM of x-ray film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Anyone out there knows how to run up an X-ray film (exposed as well as
unexposed) for TEM?????
Neelima Shah..............
Biomedical Imaging Core Facility
Uni of Pennsylvania
Philadelphia, Pa.




http://www.MED.upenn.edu/morphlab/



From daemon Wed Sep 5 18:36:20 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 05 Sep 2001 16:30:49 -0700
Subject: Re: LOMO water-immersion/dipping lenses for 160 mm-corrected

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


LOMO (Leningrad's Opto-Mechanics Consortium) is well known manufacturer of
the fine optics in the Russia. Their microscopes are cheap (relatively)
and very suitable for students etc. LOMO also known as an manufacturer of
the biggest single-piece 6 meters diameter glass mirror for the telescope
situated in Caucasus mountains. That mirror was a biggest one in the world
at the time of inventing. I believe, they did also first full-quartz-optic
microscope for UV investigations and their MB-1/2 fluorescence microscopes
with "opak-illumination" were great.

I don't have any financial interest in this company.

Sergey Ryazantsev

At 10:03 AM 9/5/01 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Sep 5 19:20:18 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Wed, 5 Sep 2001 18:13:55 -0600 (MDT)
Subject: Re: Bio. Fixation: What is Champy's Fluid?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Ah, the wonders of a Google search! I found a wonderful histo manual at

http://131.229.114.77/histo/Manual.pdf

The recipe is given on p. 17 of this pdf but it is in code - the author
lists standard histology stock solutions and then gives the recipes for a
variety of fixatives by coding them from the stock solutions. Champy's is:
"35G; 20K; 12M; 24Q(9)" which means: 35 ml of 1% chromic acid (aq.), 20 ml
of 2% OsO4 (aq.), 12 ml of sat. potassium dichromate (9 g/ 100ml, aq.), 24
ml of distilled water, make up just before use. Sounds like nasty stuff.

I'm glad you asked - I'd have never found this pdf otherwise! (It is 113
pages and I haven't been able to figure out from where it originates -
some histology course). If anyone knows who wrote it, I'd like to know!

Tamara

On Wed, 5 Sep 2001, Richard Edelmann wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} "Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed by
} ETOH dehydration, CPD, mounting and SEM viewing.
}
}
} O.k., we give up, searched numberous books, papers, catalogs, web
} sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
} "Champy's Solution"? Please?
}
} Thank you much!
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Wed Sep 5 20:27:58 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 05 Sep 2001 21:19:57 -0500
Subject: Sticky dots and Tacky Dots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I think I saw some at "Fredericks" out here on the West Coast.
Contact me offline for the number.

Earl

----- Original Message -----
} From: {"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, September 05, 2001 12:42 PM


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Scott Walck wrote:
===================================================
I was at a talk where the carbon sticky dots were first presented. I wish I
could remember who was speaking. I know that SPI has the exclusive
distribution rights to them. I believe that they were developed by a group
at Dupont. I would check the literature and see if they published. Try
back issues of the meeting abstracts. I think that the talk that I went to
was about 10 years ago.
===================================================
Let me shed some light on this: SPI Supplies was (and is) the exclusive
licensee for the manufacturing and distribution of Tacky Dot™ Slides. The
patent holder was DuPont and the inventor was Dr. Alan Cairncross, still at
DuPont. I am guessing, but the paper you heard presented (at an MSA meeting
) which could have been about ten years ago (how time flies), probably was
presented by Dr. Cairncross (and other authors all at DuPont). Full
technical details are disclosed in US Patent # 5,356,751.

These should not be confused however with what are often times called by
others as "carbon sticky dots" or "double sided conductive adhesive tabs".
These are offered in various forms by SPI as well as the other main
worldwide distributors of consumable supplies for SEM applications.

The original poster (Wharton Sinkler) asked about adhesive "tabs" but did
not specify size. The largest dot size of the Tacky Dot Slides manufactured
by SPI Supplies is 300 µm in an orthogonal array on 2000 µm centers. I
think that Wharton was in essence asking about the smallest "carbon tab" or
"disc" that is available but the smallest that I know of are are 9 mm and
there are technical reasons why one could not go too much smaller than that
(in our opinion).

Chuck


===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Wed Sep 5 21:26:23 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Thu, 06 Sep 2001 12:25:13 +1000
Subject: Re:Champy's Fluid?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Champy:

1% chromic acid 7 ml
3% K2Cr2O7 7 ml
2% OsO4 4 ml

from "Notes on microscopical technique for zoologists" CFA Pantin Cambridge
Univ. Press 1962


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400
http://srv.emunit.unsw.edu.au/


From daemon Wed Sep 5 23:26:45 2001



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wednesday, September 05, 2001 7:26 PM
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The business of un-jamming JEOL plate camera doesn't have to be that gory.
Just remove a fuse located a couple inches left of the camera door (make
sure that little red light next to the fuse turns on), before reaching
inside, and no fingers will be shed.

Assuming that the camera mechanism is not damaged- simplest thing to do to
avoid jams- do not load film boxes to a full capacity(say, load 5 or 10
holders
less), make sure that film holders are in perfect shape - ditto on Sara's
comment, toss the damaged holders- and that the film holders are loaded
correctly. Also, little Teflon
rollers on the sides of the plate boxes must rotate freely (at a slight
touch, no friction) and must have spotless surface (no defects, scratches,
etc.). Replacement rollers are available from JEOL.
If you are going to unscrew 6 screws underneath the camera compartment in
order to pull the mechanism out (as was suggested in one of the previous
messages)- beware that those screws are separating camera vacuum from the
atmosphere. Make sure that the o-rings, screws, and seats are clean before
replacing the screws, to avoid vacuum leak.

If the mechanism is damaged- repair options will depend on you mechanical
aptitude. You are welcome to contact me off-line for further advice, yet I
agree that your best bet is to contact JEOL service representative.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Marie E. Cantino {cantino-at-uconnvm.uconn.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}





From daemon Thu Sep 6 02:34:51 2001



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 6 Sep 2001 00:24:47 -0700 (PDT)
Subject: Re: Bio. Fixation: What is Champy's Fluid?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Champy's (1913) fixative formula is as follows:

sat. aqu. sol. phenol 200, 40% formaldehyde 48, trichloroacetic acid 3.6

Reference: 'The Microtomist's Formulary and Guide' by Peter Gray.
Blakiston Company, Inc., New York/Toronto. 1954:192 (794 pp.)

Cheers!
Ken

--------------
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

On Wed, 5 Sep 2001, Richard Edelmann wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} "Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed by
} ETOH dehydration, CPD, mounting and SEM viewing.
}
}
} O.k., we give up, searched numberous books, papers, catalogs, web
} sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
} "Champy's Solution"? Please?
}
} Thank you much!
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}





From daemon Thu Sep 6 06:30:09 2001



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 6 Sep 2001 12:21:47 +0000
Subject: Scanning Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know what has happened to the journal Scanning Microscopy?

The latest part we have is Volume 10 pt.4 1998 and
our supplier cannot trace the publisher etc. all mail being marked
return to sender. Has the journal ceased publishing?

Chris Jeffree

==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Thu Sep 6 07:49:33 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Thu, 6 Sep 2001 07:37:49 -0500
Subject: RE: Sticky dots and Tacky Dots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Actually, the size of the tabs is not the issue. Sorry about the confusion.

What I was asking about are indeed double-stick carbon tabs about 1 cm
diameter. I'd like to evenly space 48 of them on an aluminum plate, and am
looking for a less tedious way than by hand.

An alternative would be to simply cover the entire plate with a double-stick
conductive carbon layer, then depsoit the samples in the proper locations.
This may be cheaper than getting something custom made. Does anyone know
who might offer unperforated double stick conductive carbon material in
large dimensions (about 3 x 4 inches)?

Thanks,
Wharton

} -----Original Message-----
} From: Garber, Charles A. [SMTP:cgarber-at-2spi.com]
} Sent: Wednesday, September 05, 2001 9:20 PM
} To: MICROSCOPY BB
} Subject: Sticky dots and Tacky Dots
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Scott Walck wrote:
} ===================================================
} I was at a talk where the carbon sticky dots were first presented. I wish
} I
} could remember who was speaking. I know that SPI has the exclusive
} distribution rights to them. I believe that they were developed by a
} group
} at Dupont. I would check the literature and see if they published. Try
} back issues of the meeting abstracts. I think that the talk that I went
} to
} was about 10 years ago.
} ===================================================
} Let me shed some light on this: SPI Supplies was (and is) the exclusive
} licensee for the manufacturing and distribution of Tacky Dot(tm) Slides.
} The
} patent holder was DuPont and the inventor was Dr. Alan Cairncross, still
} at
} DuPont. I am guessing, but the paper you heard presented (at an MSA
} meeting
} ) which could have been about ten years ago (how time flies), probably was
} presented by Dr. Cairncross (and other authors all at DuPont). Full
} technical details are disclosed in US Patent # 5,356,751.
}
} These should not be confused however with what are often times called by
} others as "carbon sticky dots" or "double sided conductive adhesive tabs".
}
} These are offered in various forms by SPI as well as the other main
} worldwide distributors of consumable supplies for SEM applications.
}
} The original poster (Wharton Sinkler) asked about adhesive "tabs" but did
} not specify size. The largest dot size of the Tacky Dot Slides
} manufactured
} by SPI Supplies is 300 µm in an orthogonal array on 2000 µm centers. I
} think that Wharton was in essence asking about the smallest "carbon tab"
} or
} "disc" that is available but the smallest that I know of are are 9 mm and
} there are technical reasons why one could not go too much smaller than
} that
} (in our opinion).
}
} Chuck
}
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================
}


From daemon Thu Sep 6 08:31:41 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Thu, 6 Sep 2001 09:24:09 -0400
Subject: Re: Bio. Fixation: What is Champy's Fluid? Found.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thank you for all who replied we now have a recipe to try out.

Champy's:

=========

1% chromic acid 7 ml
3% K2Cr2O7 7 ml
2% OsO4 4 ml

from "Notes on microscopical technique for zoologists" CFA Pantin
Cambridge
Univ. Press 1962

========

"[3% aqueous potassium dichromate; 1% aqueous chromic acid; 2%
aqueous solution of osmic acid (osmium tetroxide); (2:2:1)]" Page 804-805
Samuel Thompson's "Selected Histochemical and Histopathological
Methods", 1966.

==========

"35G; 20K; 12M; 24Q(9)" which means: 35 ml of 1% chromic acid (aq.), 20
ml of 2% OsO4 (aq.), 12 ml of sat. potassium dichromate (9 g/ 100ml, aq.),
24ml of distilled water, make up just before use. Sounds like nasty stuff.

===========



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Thu Sep 6 09:25:41 2001



From: Jeff Hedlesky :      jhedlesky-at-edgebb.net
Date: Thu, 06 Sep 2001 09:18:06 -0500
Subject: Feigl's Solution?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone out there know: a) where to buy this stuff? b) How to
make this stuff? or c) where I can find any articles, mentions or
instructions on the use of this stuff? (other than the Fisheries
Science article that comes up in Google) I've been told that it's a
useful marker for the aragonite polymorph of CaCO3. (Thanks, John Hunt
at CCMR!) Any feedback, however oblique, would be appreciated.

Jeff

--
Jeff Hedlesky
Aladdin Companies
151 N. Main, Suite 700
Wichita, KS 67202

316.265.8913 voice
316.265.7014 FAX
jhedlesky-at-edgebb.net



From daemon Thu Sep 6 09:25:44 2001



From: Bradley Starcevich :      microscopist-at-excite.com
Date: Thu, 6 Sep 2001 07:17:39 -0700 (PDT)
Subject: Lomo optics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


My experience with LOMO microscopes and optics
has been positive as well. Their products are
an incredible value, the quality is very good.

I'd buy another LOMO product in a heartbeat.

I do not represent LOMO. I have no financial
interest in the company.

Bradley K. Starcevich
Director, Research and Development
The Cougar Group
1215 Atlantic Drive
West Chicago, IL USA 60185





_______________________________________________________
http://inbox.excite.com




From daemon Thu Sep 6 11:29:05 2001



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Thu, 6 Sep 2001 11:18:09 -0500
Subject: SEM: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
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Hello to the list.

It's been a while since I've been on the list, and since I've been working
as a microscopist.

If I image a sample in the SEM with the sample tilted, then want to make
measurements from the images, what is the equation to use to correct for the
tilt? I know it involves the cosine of the tilt angle, but beyond that, I'm
drawing a blank.

Thanks for your assistance.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Thu Sep 6 12:30:25 2001



From: Axel Blau :      axel-at-caltech.edu
Date: Thu, 6 Sep 2001 10:22:08 -0700
Subject: Re: LOMO water-immersion/dipping lenses for 160 mm-correctedscopes

Contents Retrieved from Microscopy Listserver Archives
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Thanks to the two of you for the information. The website -at-
www.comet.net/gek/ is very interesting, and I did not know that there was
some other distributor in the US. I have to agree that the quality of the
lenses we got is outstanding for the price we paid.

With best regards,

Axel

----- Original Message -----
} From: Jeff Hedlesky
To: Sergey Ryazantsev
Cc: Axel Blau ; Microscopy-at-sparc5.microscopy.com
Sent: Thursday, September 06, 2001 6:56 AM





From daemon Thu Sep 6 12:34:07 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 6 Sep 2001 13:28:48 -0400
Subject: SEM: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
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For a flat sample with tilted to an angle, theta, the image perpendicular to the tilt axis is foreshortened by cos(theta). Take your measurement on the tilted image and divide by cos(theta).

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Nancy Zjaba [mailto:nzjaba-at-alfalight.com]
Sent: Thursday, September 06, 2001 12:18 PM
To: 'microscopy-at-msa.microscopy.com'


Hello to the list.

It's been a while since I've been on the list, and since I've been working
as a microscopist.

If I image a sample in the SEM with the sample tilted, then want to make
measurements from the images, what is the equation to use to correct for the
tilt? I know it involves the cosine of the tilt angle, but beyond that, I'm
drawing a blank.

Thanks for your assistance.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Thu Sep 6 12:49:30 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 06 Sep 2001 12:42:18 -0500
Subject: Re: SEM: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
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After tilting, the feature you want to measure will form the hypotenuse of
a triangle. The horizontal projection you see will be the side of the
triangle adjacent to the tilt angle. One side of the feature will be
elevated with respect to the other and that amount will be the length of
the side opposite the tilt angle.

The cosine of an angle is the ratio of the length of the adjacent side to
the length of the hypotenuse. Therefore, you would use the following formula.

hypotenuse = adjacent/cosine, or

real length - observed length/cosine(tilt angle).

At 11:18 AM 9/6/2001 -0500, you wrote:

} Hello to the list.
}
} It's been a while since I've been on the list, and since I've been working
} as a microscopist.
}
} If I image a sample in the SEM with the sample tilted, then want to make
} measurements from the images, what is the equation to use to correct for the
} tilt? I know it involves the cosine of the tilt angle, but beyond that, I'm
} drawing a blank.
}
} Thanks for your assistance.
}
} Nancy Zjaba, Technical Staff
} Alfalight Inc.
} 1832 Wright Street
} Madison, WI 53704
} (608) 240-4875
} nzjaba-at-alfalight.com



From daemon Thu Sep 6 13:28:30 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 06 Sep 2001 11:21:58 -0700
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I was working with JEOL microscopes (100B, 100C, 100CX, 1200EX) for a few
decades. Over all this time I do remember just a few accidents related to
the camera mechanics in the microscopes. In all that cases the "human
factor" was involved and usually the problem was improperly loaded into
cassette the piece of the film. If film is not in place, sometime it cover
one of the square slots on the cassette and "fork" could not catch the
cassette properly. In this case the forks usually stick in the gap between
two set of rails in the camera mechanism. As it was mentioned in the most
replies, the solution is to move up those forks out of the gap. Usually I
am doing so using flexible metal ruler with fuse OFF (great comment
Vitaly). The bad things about JEOL camera design that it has extremely
powerful motor with huge gear which is able to bent and ruin all rails in
the camera if working improperly. Magically, it was never happens with my
microscopes, but when I look inside the camera and see that huge gears I am
worrying anyway.

Sergey

At 12:12 AM 9/6/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Sep 6 14:35:40 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 06 Sep 2001 15:26:37 -0500
Subject: Large Double Sided Adhesive Conductive Carbon Sheets

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Wharton Sinkler wrote:
=================================================
Actually, the size of the tabs is not the issue. Sorry about the confusion.

What I was asking about are indeed double-stick carbon tabs about 1 cm
diameter. I'd like to evenly space 48 of them on an aluminum plate, and am
looking for a less tedious way than by hand.

An alternative would be to simply cover the entire plate with a double-stick
conductive carbon layer, then depsoit the samples in the proper locations.
This may be cheaper than getting something custom made. Does anyone know
who might offer unperforated double stick conductive carbon material in
large dimensions (about 3 x 4 inches)?
==================================================
We at SPI Supplies offer as a standard off-the-shelf product a sheet that is
500mm x 500 mm. You could cut out from that standard size sheet whatever
size you might require. This is described on URL
http://www.2spi.com/catalog/spec_prep/cond_adhes-sheets.html

This is the "master material" from which the double sided conductive carbon
adhesive discs are fabricated.

We have heard of another customer who has made a template in the orthogonal
arrangement required and then by hand is affixing (on large sheets) what
they want to attach through the use of the template, which is suspended
slightly above the adhesive and never actually contacts the adhesive. That
would be the cheapest of the various alternatives.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Thu Sep 6 14:53:19 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 6 Sep 2001 15:46:24 -0400
Subject: Re: About working with fixatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
with a Teflon lined screw cap. The bottle is then held in the refrigerator
in a small plastic jar (available from all lab distributors - mine from
Fisher or S/P) with a screw cap . In this way, the plastic becomes
osmicated NOT the refrigerator. I have done this for years with no untoward
results.

I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass or
plastic. Osmication is done in glass vials with Teflon lined screw caps
using a minimum of OsO4 to do the job.

I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
Teflon lined cap. I then make up an appropriate amount of fixative using
the 10% glu and 10X buffer concentrate where possible. The 10% Glu is kept
in a plastic jar in the refrigerator.

I find that double containers are safe and reduce contamination
considerably.

Hope this helps.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



From daemon Thu Sep 6 15:45:37 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Thu, 06 Sep 2001 13:37:41 -0700
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




From daemon Thu Sep 6 16:25:34 2001



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu
Date: Thu, 6 Sep 2001 16:18:19 -0500
Subject: Drosophila brain fixation

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,
I have a researcher here that is having some difficulty with getting good
histology at the EM level. She is fixing Drosophlia brains with 4%para/0.5%
glut in phosphate buffer pH7.2, no osmium post fix, ethanol dehaydration,
propylene oxide as a transitional solvent to polybed 812, then performing post
sectioning immunolabeling. The tissue has "holes" in it when viewed at the
TEM. They are not holes in the section, but appear to be voids in the tissue.
We have tried leaving out the propylene oxide, we have osmicated, and have
looked at osmotic strength of the fixative (and many variations of the above,
to no avail). Can anyone offer advice?
Randy Nessler
Univ. of Iowa



From daemon Thu Sep 6 16:25:52 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 6 Sep 2001 16:19:20 -0500
Subject: RE: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
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R = T/(M*cosA)

R - "real measurements"
T - measurements on a tilted specimen
A - angle
M - magnification

But you can use it only if your specimens is flat.
Otherwise you should use stereo measurements.

Good luck,

Vladimir



Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Nancy Zjaba [mailto:nzjaba-at-alfalight.com]
} Sent: Thursday, September 06, 2001 11:18 AM
} To: 'microscopy-at-msa.microscopy.com'
} Subject: SEM: Tilt correction factor
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hello to the list.
}
} It's been a while since I've been on the list, and since I've
} been working
} as a microscopist.
}
} If I image a sample in the SEM with the sample tilted, then
} want to make
} measurements from the images, what is the equation to use to
} correct for the
} tilt? I know it involves the cosine of the tilt angle, but
} beyond that, I'm
} drawing a blank.
}
} Thanks for your assistance.
}
} Nancy Zjaba, Technical Staff
} Alfalight Inc.
} 1832 Wright Street
} Madison, WI 53704
} (608) 240-4875
} nzjaba-at-alfalight.com
}
}
}
}


From daemon Thu Sep 6 16:55:02 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 06 Sep 2001 16:47:21 -0500
Subject: Ethanol vs. Acetone for critical point drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Recently, a collegue was having problems with a white crystaline
substance covering his samples of ear tissue after they were removed
from the critical point dryer. It was believed to be from a
contaminant in the ethanol and it was recommended to switch to acetone
dehydration as it was less likely to have this problem. I haven't done
SEM in years, so I haven't been paying attention to anything like this
on this listserve. I was wondering if other people experiencing this
problem. If you are using acetone, have you ever seen this?

Thanks for your comments,

Karen Pawlowski, Ph.D.


From daemon Thu Sep 6 18:03:13 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 6 Sep 2001 18:26:24 -0500
Subject: Re: Ethanol vs. Acetone for critical point drying

Contents Retrieved from Microscopy Listserver Archives
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Depends upon the geometry of the sample: try making the calculations for a
sphere at a 45 degree angle.

My guess is that the measurements would be the same as if the sphere were at
0 degrees or 90 degrees.

For purely planar sample the equations would be valid.

regards,

Earl

----- Original Message -----
} From: "Nancy Zjaba" {nzjaba-at-alfalight.com}
To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, September 06, 2001 9:18 AM


Karen,

One possible source for this contaminant is from the use of drying
agents such as molecular sieves or other crystals. Unless one is
extremely careful, the fine crystals get suspended in the liquid
phase and dry down onto the specimen. We no longer use drying agents
in our ethanol but use absolute ethanol directly from small (400-500
ml) bottles. If the bottles have been opened for awhile, then we use
the alcohol to prepare a dehydration series since it is no longer
anhydrous.

You CAN still use molecular sieves, etc. but we prepare them one
month before use and take care not to jostle the bottles to any
extent.

Another source for the crystalline material may be from prior
contamination of the chamber by someone who had a dirty sample or
contaminated ethanol.

JB



} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Sep 6 18:35:58 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 06 Sep 2001 21:57:47 -0400
Subject: Un-jammed and thanks

Contents Retrieved from Microscopy Listserver Archives
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The address for your LOMO dealer is:
Microscopy Labs
Box 338
Red Bank, NJ 07701
732 747 6228
fax 732 758 9142
----- Original Message -----
} From: "Bradley Starcevich" {microscopist-at-excite.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, September 06, 2001 10:17 AM


Once again, my plea for help to you friendly and knowlegeable folks on
this listserve produced twelve hints, warnings to protect my fingers and
encouragement to keep smiling through equipment hassles. I have compiled
them into a lengthy word document and would be happy to send it to anyone
requesting it. The following were the most important bits of advice I
received from all of you and from a discussion with one of JEOL's excellent
service techs working out of Pittsburgh
a) the camera mechanics are pretty much the same (100, 1200, 2010 even)
b) if an error message indicating the "no film loaded" appears on the
computer screen, remove the fuse which turns the mechanism's motor off
(preventing the motor from burning out and your fingers should you elect
to remove film + plate)
c) examine plates--this one was "bowed" and showed a wear pattern
d) don't load the film box to capacity
e) dispose of damaged plates, even those with minor bends
f) examine the teflon rollers on the film boxes, replace if they do not
rotate freely

Thanks again to: Sergey Ryazantsev, UCLA; Robin Cross, Rhodes University,
South Africa; Keith Moulding, Hong Kong University of Science and
Technology, Hong Kong; Deb Stenzel Queensland Univ. of Tech, Brisbane,
Australia: . John Hunt, Cornell: John Humenansky/Staff Scientist Physical
Electronics, Inc. (PHI); Malcolm Haswell, UK; Richard Beanland, Structural
Analysis Lab,Caswell Technology; Sara Miller, Duke University; Wilbur C.
Bigelow, University of Michigan; Marie E. Cantino, University of
Connecticut; Vitaly Feingold Scientific Instruments and Applications.

Rosemary Walsh, PSU







From daemon Fri Sep 7 04:43:07 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 7 Sep 2001 10:30:55 +0100 (GMT Daylight Time)
Subject: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


While we are on the topic of making up fixatives I have a
supplementary question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a
glass rod. This is nerve-wracking, tedious (they do not
break readily) and not as safe a procedure as I would like.

The ampoules used to have a weakened neck which could be
sawn off but they do not come in this form from our
supplier any more.

Is there an easier way?

Dave


On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
} with a Teflon lined screw cap. The bottle is then held in the refrigerator
} in a small plastic jar (available from all lab distributors - mine from
} Fisher or S/P) with a screw cap . In this way, the plastic becomes
} osmicated NOT the refrigerator. I have done this for years with no untoward
} results.
}
} I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass or
} plastic. Osmication is done in glass vials with Teflon lined screw caps
} using a minimum of OsO4 to do the job.
}
} I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
} HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
} Teflon lined cap. I then make up an appropriate amount of fixative using
} the 10% glu and 10X buffer concentrate where possible. The 10% Glu is kept
} in a plastic jar in the refrigerator.
}
} I find that double containers are safe and reduce contamination
} considerably.
}
} Hope this helps.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Sep 7 04:43:11 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 7 Sep 2001 19:35:43 +1000
Subject: RE: Ethanol vs. Acetone for critical point drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Using clean ethanol (does not need to be "dried" for CPD) would be a lot easier
than replacing seals - if the valves in your CPD don't like acetone.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, September 07, 2001 7:47 AM, Karen Pawlowski
[SMTP:kpawlow-at-swbell.net] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.


From daemon Fri Sep 7 05:35:35 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 6 Sep 2001 16:53:08 -0400
Subject: RE: Camera Lucida drawing tube (Zeiss) questions

Contents Retrieved from Microscopy Listserver Archives
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I would first like to thank the list members for the responses I have
received. Thanks are also due to software support at Synoptics for a
prompt and informative reply to my e-mail. I provide here a brief
description of how the problem was resolved finally, in the hope that
the info may be useful to somebody someday, just as I have learned a
lot from this list.

On checking the binary file from the SGI computer in a hex viewer, I
confirmed that the bytes were not in the format required by Semper for
a Fortran unformatted file. Also confirmed that this was not due to
file transfer by explicitly setting the transfer mode in ftp to binary.
The e-mail from Synoptics informed that the Fortran unformatted format
was not intended to be portable across operating systems and different
hardware and that the input / output commands in Semper supersede the
read / write commands. I discovered that the format is not even
portable from the DOS version to the Windows version of Semper. The
main suggested solution was to convert the image to one of the standard
binary image formats such as tiff that can be read into Semper.

Two solutions are possible:
(i) the tv1 program in EMS can be used to display the EMS image and the
screen can be exported to Semper format. This export file has the
extension eix and is an ASCII file with the image pixel data as
integers. When read into Semper it is available as a byte class image.
Problem with this approach is that the pixel data floating point values
are converted to 8-bit values and I am not sure if this affects further
quantification. Also, it requires selecting the area to be saved and I
tend to end up with odd sized images which included bits of the screen
background requiring further cropping with Semper.

(ii) To change the source code of se1 such that all unformatted Fortran
write statements are replaced with formatted write with the format
strings as per Semper file format specifications. On compiling and
running, this results in an ASCII file which can be read into Semper as
a floating point image, that is, without any change in the pixel
values. This is the solution I have adopted. The resulting file size is
larger by about 3.5 times but this is not a limitation for me. I am
still left with the minor problem that the title of image is getting
written as a string of blank characters. My Fortran is a bit rusty and
I hope to solve this soon.

Best Wishes to all.

-----Original Message-----
} From: Divakar [SMTP:divakar-at-igcar.ernet.in]
Sent: Tuesday, September 04, 2001 10:33 AM
To: 'Microscopy-at-sparc5.microscopy.com'


Hi Alan,
Go get em! I have a Camera Lucida for a Leitz Ortholux that I have
sitting next to me in my office.
The collar that I have fits over the narrow diameter eyepiece tube
on my scope and sets my Camera Lucida at the exit pupil of the ocular. This
permits very close concordance of the two images transmitted by the prism.
Of course, my camera Lucida is an old thing with a 4x6" mirror hanging off
to the right. My drawing surface must be inclined, because my oculars are
inclined on my binocular headed microscope. I had to construct the
inclined table, but it works anyway.
Here's a project. Take a piece of paper and mark it off in half
centimeters (i.e., every 5mm, Ho! Ho!). Using a high mag objective look at
something small like a diatom. Using the graduations on the fine focus knob
change focus by one graduation and move the paper one 5mm increment drawing
the outline of the in-focus diatom at each point. You will quickly see how
to limit your lines so they don't overlap. Try to predict what the result
will be after you draw several outlines.
The key to getting everything right is to balance the illumination
of the paper with that of the scope, but you probably noticed that already.
Send me a photo by direct eMail. The listserver doesn't convey
attachments.

You are welcome to ask questions any time or to discuss,
What kind of scope(S) do you have? Illuminators? Use high intensity on the
paper!


Regards,

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail (Work): fmonson-at-wcupa.edu
eMail (Home): monson_fc-at-msn.com
Please call before visiting



} ----------
} From: Alan Davis
} Sent: Wednesday, September 5, 2001 7:16 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Camera Lucida drawing tube (Zeiss) questions
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have gotten ahold of a Zeiss camera lucida drawing tube. It has a
} mirror at an angle that allows one to draw underneath the inclined eye
} tube. Works for a Zeiss Standard 16. I am getting the hang of it a bit.
} However, I have at least one question:
}
} What is the collar for (referred to as "ring clamp" below)? It is
} somewhat eccentric, but I cannot imagine any purpose for it. The device
} doesn't fit over it, but nearly does. It *is* useful for attaching it to
} a wider eyepiece tube.
}
} This plastic collar fits snugly inside the eyepiece tube of an Olympus
} SZH; this is good. Also, it fits over the end of the standard 16 eye
} tube, with a barrier that doesn't allow it to slip down over the tube. i
} have some documentation for a similar drawing tube, but there is a slight
} difference in the picture (there is a knurled knob at the point where the
} mirror/prism fits over the eyepiece. Mine doesn't have that, it fits
} snug). Here is the relevant section from the documentation for the
} pictured device:
}
} 1. Focus microscope.
}
} 2. Remove eyepiece and slip ring clamp on to tube. Replace eyepiece
} and fix with ring clamp. [I referred to the "ring clamp" as a
} collar.]
}
} 3. Clamp drawing prism on the eyepiece. [I assume this refers to the
} knurled
} screw that isn't present on my device---mine doesn't need to be
} clamped on.]
}
}
}
} } From reading the description, though, I don't know what it is for. Is it
} a spacer to move the eyepiece up higher? Which Zeiss is it really
} designed for?
}
} Also, I would greatly appreciate any tips. The tips I found in MICSCAPE
} were helpful. Can I initiate some correspondence with someone who has
} some experience with one of these fantastic devices?
}
} Alan Davis
} Marianas High School
}
} --
} adavis-at-saipan.com 1-670-235-6580
} Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI
}
} I have steadily endeavored to keep my mind free, so as to give up any
} hypothesis, however much beloved -- and I cannot resist forming one
} on every subject -- as soon as facts are shown to be opposed to it.
} -- Charles Darwin (1809-1882)
}
}
}
}
}
}


From daemon Fri Sep 7 06:20:46 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 6 Sep 2001 17:57:48 -0400
Subject: Re: Plate Jam is Tasty

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


According to Prof Bigelow, one clears such a jam with a wiggle! But
from the Eastern Hemisphere and Dr. Moulding we hear that such jams are
freed by a waggle. Now what needs to be established is whether one should
wiggle or waggle, or whether one must learn to do both, AND whether TEM's
behave differently toward wiggling and waggling in different places (We all
know JEOL is 100V no matter where you find it.).
Having been raised in North America, I have been exposed to what has
always been described as typical wiggling, and I would hope that if I ever
saw someone waggle, I would know that waggling was being done. One might be
terribly embarrassed if one were to characterize a wiggle as a waggle. For
example, is there wiggling or waggling in typical down-under dancing? If
one stands in Hong Kong ogling the girls as they pass by, is one traumatized
by the wiggle or the waggle?
We know that one can wiggle a nose, but conversely one can only wag
one's tail - in North America. But then, we all have seen buns wiggle.
Perhaps
the relationship is something like this. A waggle could be a small wag, and
since we know that Prof. Bigelow was not suggesting that one should wag the
plate
to free it, rather he suggested that we just give it a little wiggle. In
this way one could assert no difference between a wiggle and a waggle, both
being, similarly, small wags. One would not say, "Wag the plate a little."
But one could "Waggle it!" or just "wag it a little!" This would all be so
much more clear if dogs in
North America were known to "wig their tails" when they are happy. Perhaps
what we have is an hemispheric etymological inversion such that in the
West, wiggle is to wag, where as, in the East, waggle is to wig!
We here in the Western Hemisphere have learned to watch worms
wiggle, but what do they do elsewhere, I wonder? Might a waggle merely be
out of phase with a wiggle? Sine! Cosine! Do these differences occur,
because of the differences between 50 and 60 Hz? Perhaps there might be NSF
or WHO money for such a question.

Oh well, ruminating like a mad sow, he demurred, "No rumen in the old sow at
all."

If anyone can clear any of this up, I certainly would like to know!

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



From daemon Fri Sep 7 07:30:36 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 7 Sep 2001 08:21:24 -0400
Subject: RE: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Always an interesting question.

While I have usually come to a comfortable resolution with respect
to this kind of safety issue, this one has proved elusive. So.....

My normal procedure is to unwrap and clean the ampoule as I would my
best glassware. That is, I treat it to a "biochemistry lab" cleaning,
finishing with 3X rinses in sgdd(single glass-distilled deionized) HOH.
Between the last two waters AND WEARING FRESH POWDER-FREE GLOVES, I score
the ampoule three times with an ampoule file while holding it on a folded
paper towel in the bottom of a shallow heavy-glass bowl. I then transfer
the ampoule to an appropriate 100ml bottle (I use a serum bottle, because it
is constructed of thick glass, shows imperfections readily (especially
around the bottom), and can withstand the breaking of the ampoule). The
scored ampoule breaks more readily than an unscored one, and three scores
(~1cm apart) provide sufficient weakness for breakage to occur with relative
ease. To further facilitate breakage, I add only some of the total amount
of solvent HOH (for me, 25ml) sufficient to keep the ampoule from floating
while providing enough for initial immersion of the crystals. Finally, I
rest the serum bottle on the same folded paper towel in the same thick glass
bowl to dissipate the shock of the breaking regimen. NOTE: Since it is at
this part of the process at which failure has occurred (twice in 25 years
the bottom of the bottle has separated from the barrel), I am still working
in the hood, but with a substantial amount of 95% EtOH at hand to render the
OsO4 harmless if I have an accident.

If the above smacks of science, please forgive the allusion. The
method and approach are the result of what has evolved over time through
trial and error and the application of common sense to a continuing, natty
problem.

I have always hoped that someone would supply a FINAL solution so we
can all be more comfortable when "Stock solution" time arrives.

Regards,

Fred Monson




} ----------
} From: Patton, David
} Sent: Friday, September 7, 2001 5:30 AM
} To: Monson, Frederick C.
} Cc: 'Microscopy Listserver'
} Subject: Question about preparing osmium tetroxide
}
} While we are on the topic of making up fixatives I have a
} supplementary question.
}
} How do you open glass osmium tetroxide ampoules?
}
} I put mine in a 100ml glass bottle and then break it with a
} glass rod. This is nerve-wracking, tedious (they do not
} break readily) and not as safe a procedure as I would like.
}
} The ampoules used to have a weakened neck which could be
} sawn off but they do not come in this form from our
} supplier any more.
}
} Is there an easier way?
}
} Dave
}
}
} On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
} {fmonson-at-wcupa.edu} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I dissolve 1g OsO4 in 25ml double distilled water and store it in a
} bottle
} } with a Teflon lined screw cap. The bottle is then held in the
} refrigerator
} } in a small plastic jar (available from all lab distributors - mine from
} } Fisher or S/P) with a screw cap . In this way, the plastic becomes
} } osmicated NOT the refrigerator. I have done this for years with no
} untoward
} } results.
} }
} } I fix with aldehydes in screw cap scintillation vials (20ml or 5ml)
} glass or
} } plastic. Osmication is done in glass vials with Teflon lined screw caps
} } using a minimum of OsO4 to do the job.
} }
} } I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml
} dd
} } HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with
} a
} } Teflon lined cap. I then make up an appropriate amount of fixative
} using
} } the 10% glu and 10X buffer concentrate where possible. The 10% Glu is
} kept
} } in a plastic jar in the refrigerator.
} }
} } I find that double containers are safe and reduce contamination
} } considerably.
} }
} } Hope this helps.
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging(CASI)
} } West Chester University of Pennsylvania
} } Schmucker Science Center II
} } South Church Street
} } West Chester, PA, 19383
} } eMail: fmonson-at-wcupa.edu
} } http://darwin.wcupa.edu/casi/
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}


From daemon Fri Sep 7 08:02:19 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 7 Sep 2001 14:48:48 +0200
Subject: Re: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Depends also from the measurement direction. The formula is right in the
tilt direction, but the real tilt angle of a straight line on flat sample
varies from nominal tilt angle in tilt direction to zero in the
perpendicular direction. In the other directions, the formula is right
too, but you must estimate the real tilt angle in the measurement
direction, in respect to the tilt direction.

And of course, don't forget than in a SEM the perspective is not "conique"
(I don't know the right terms in english) but "cavalière" . A tilted
square looks like a rectangle and not a trapezoid. Try with a TEM grid
with square holes, and try too with a grid with hexagonal holes. And
rotate it ... You may feel dizzy, with high tilt angles. Our brain is not
build for such a perspective ! But it can give nice pictures.



J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Thu, 6 Sep 2001, Earl Weltmer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Depends upon the geometry of the sample: try making the calculations for a
} sphere at a 45 degree angle.
}
} My guess is that the measurements would be the same as if the sphere were at
} 0 degrees or 90 degrees.
}
} For purely planar sample the equations would be valid.
}
} regards,
}
} Earl
}
} ----- Original Message -----
} } From: "Nancy Zjaba" {nzjaba-at-alfalight.com}
} To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, September 06, 2001 9:18 AM
} Subject: SEM: Tilt correction factor
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello to the list.
} }
} } It's been a while since I've been on the list, and since I've been working
} } as a microscopist.
} }
} } If I image a sample in the SEM with the sample tilted, then want to make
} } measurements from the images, what is the equation to use to correct for
} the
} } tilt? I know it involves the cosine of the tilt angle, but beyond that,
} I'm
} } drawing a blank.
} }
} } Thanks for your assistance.
} }
} } Nancy Zjaba, Technical Staff
} } Alfalight Inc.
} } 1832 Wright Street
} } Madison, WI 53704
} } (608) 240-4875
} } nzjaba-at-alfalight.com
} }
} }
} }
}
}



From daemon Fri Sep 7 08:12:57 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Fri, 7 Sep 2001 09:11:37 -0400
Subject: Re: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The method we use, which I teach to all the students, is to wrap the vial
with a short length of paper towel (usually about four or five turns around
the bottle), grasp the vial at both ends, and gently exert pressure with
the thumbs. Then carefully unwrap the package and throw both vial halves
in warm distilled water. I've never had any problem, either with crystals
falling out in the paper or with glass poking through the paper towels. I
was also taught the whack method, and found that my aim was not adequate to
the job.

We use the same wrapping method with glutaraldehyde ampoules, but instruct
the students to be sure to keep them upright.

Marie
}
} While we are on the topic of making up fixatives I have a
} supplementary question.
}
} How do you open glass osmium tetroxide ampoules?

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Fri Sep 7 08:17:08 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 7 Sep 2001 14:14:45 +0100 ()
Subject: Re: SEM: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



To Nancy Zjaba and all Listers,

Regarding tilt correction, I enclose this from an earlier reply: it is a
warning against the use of the tlit correction control on the SEM.

*** one thing is that [certain] morphology is generally quite low-profile
and vague under SEM, _unless_ you tilt the specimen: I generally tilt at
54^ = ARC COS (1 / SQRT(3)), the "magic" angle which ensures that x, y,
and z come out isometric, with the axes at 120^ to each other: if there
is any orientation (say in an injection moulding or drawn specimen, then
while the specimen is still flat-on, make sure that x and y are both at
45^ to the tilt axis. Then you will get isometric projection of all three
axes. But NEVER use the tilt-correction control, or you will get
distortion: for example, it makes spheres (which should appear circular at
any angle) come out as ellipsoids.


+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+







From daemon Fri Sep 7 08:32:58 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 7 Sep 2001 08:26:14 -0500
Subject: Re: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have a diamond pen or scribe (bought mine from EMS) that I normally
use for indelibly etching numbers on glass slides. I use it to
carefully scratch a circular line completely around the narrowmost
point of the ampoule. I usually go around several times to ensure a
good scratch. I rinse the vial off with 95% ethanol and place inside
a sturdy glass bottle (I use a Schott-brand glass bottle with orange
plastic cap - available from major supply houses like fischer). I
then shake the bottle vigorously (inside the fume hood, while
silently praying it doesn't break!) It generally breaks within a few
good snaps of the wrist. I add 50 mls of water and then store this
bottle inside a plastic jar in my fume hood. The inside top of the
plastic cap of the Schott bottle and the inside of the plastic jar go
black with time and I periodically (yearly) replace them. I would
never trust putting this stuff in my refrigerator. It leaks out the
first bottle - why would one think it doesn't then leak out the
second? I use it up usually within 3-4 months and never had a
problem with room temperature storage over the last 20 years. If you
use ampoules with pre-scored lines (easy snap open style) there is
another trick to consider i used to use in the old days but it comes
with a potential hazard that safety officers and lawyers advise
against so I won't recommend it. Place about one cm of liquid
nitrogen in a 50 ml disposable beaker in the hood. Slowly lower the
ampoule into the liquid nitrogen. this causes the osmium to contract
and break free from the glass. Snap the ampoule and pour the
contents into water. the low temp also reduces fumes during pouring.
this method avoids glass in your stock solution. the danger is that
if there is a crack in your vial, the nitrogen would enter, and when
warmed, potentially explode the vial. I never tried dry ice but i
suspect it would work and avoid this hazard.

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Sep 7 08:54:28 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 7 Sep 2001 08:52:19 -0500
Subject: Re: Ethanol vs. Acetone for critical point drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Karen,

I have done CPD for years, and never seen this problem with EtOH
dehydration (or acetone). The only times I've gotten anything like
this is from the specimen container used for drying, or salt
precipitation when the buffer was incompletely washed off. Another
possibility is the CO2. Many CO2 tanks are full of water and crud. Is
there a molecular sieve filter (for the water) and a membrane filter
(for the crud) on the line from the tank to the CPD?

Mind, it contaminant *could* be from the EtOH, but that's just a
reason to use better EtOH, not to switch to acetone.

Phil

} Hi,
}
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Sep 7 10:30:21 2001



From: Krueger, Eugene W. :      krueger.eugene-at-mayo.edu
Date: Fri, 7 Sep 2001 10:23:26 -0500
Subject: 3-d reconstructions from serial sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
I was wondering what computer programs people are using for 3-D
reconstruction of TEM serial sections. I am interested in peoples opinions
of the software (i.e. ease of use, learning curve, cross-platform
functionality, cost, etc.). Feel free to email me off list if you choose.
Also vendors feel free to send me any info you might have on the products
you sell.

Thanks to all,

-EK

Eugene W. A. Krueger
Sr. Research Technologist in Cell Biology II
Center for Basic Research in Digestive Diseases
Mayo Clinic and Foundation
(507)284-0580 (lab)
(507)284-0762 (fax)
krueger.eugene-at-mayo.edu

see our web page at http://www.mayo.edu/mcniven_lab/


From daemon Fri Sep 7 13:54:49 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Fri, 7 Sep 2001 14:45:06 -0400
Subject: Re: Ethanol vs. Acetone for critical point drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Karen:

Another source that we have come across is sodium phosphate buffer
crystals. If fixation is carried out with sodium phosphate buffered soluitons,
and then immediately transittioned to dehydration solvent (either EtOH or
Acetone) then we have seen samples coated with fine crystals. We use
either serveral water rinses between fixation and dehydration or an alternative
buffer (i.e. Na Cacodylate)

}
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Fri Sep 7 14:13:11 2001



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Fri, 07 Sep 2001 12:05:01 -0700
Subject: Electron Microscopes: Electric Trains, Buses, Subway

Contents Retrieved from Microscopy Listserver Archives
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I would be interested in hearing from people anywhere in the world who have
had PERSONAL experiences with the installation of electron microscopes or
other sensitive equipment within 800 meters of electrically powered light
rail, trains, buses or subway systems which have created vibrations or
stray field problems for these instruments. I am specifically interested
in hearing from those who have had prior experiences with light rail or bus
transportation units powered by electricity. I would like to hear positive
(no known problems for sensitive instruments) as well as negative
experiences you have had or are having.

If you are aware that one or more of these types of transportation systems
is within the 800 meter range of your equipment and specific measures were
taken to eliminate vibration or stray fields, I will like to know that too.


You may wish to respond to me personally. Thank you.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Fri Sep 7 14:55:48 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Friday, September 7, 2001 4:30 AM
Subject: Fwd: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dave,
I personally would not use a supplier who does not pack Osmium in easy open vials or ampoules such as the ones which have the thin neck that can be snapped open. It is just too dangerous to try to break the vials as you described.

Often the osmium crystals will adhere tightly to the glass. If you dip the vial into liquid nitrogen while holding it by the neck with forceps, the crystals will release from the glass surface. They can then be shaken down to one end of the vial.

You can purchase silicon ampoule breakers from some of the major EM supply houses. They are flexible molded silicon with a depression the shape ofthe ampoule. Just put the ampoule in, bend the mold and the ampoule will break. Then remove the portion with the crystals and pore them into your osmium container. You end up with the two large piece of glass which should be disposed of carefully since there might be traces of osmium remaining. The osmium crystals will normally dissolve into water (or buffer) if left overnight at room temperature....in a hood.

I normally make osmium up double strength in water and it will keep for months in the refrigerator, remaining a clear light amber solution. I also recommend double bottling to help prevent escape of vapors. However, we do not store any biologicals in the same refrigerator with fixatives. Regardless of how careful you try to be, the odds are that some fixative vapors will escape.

Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907



--------------------------------------


While we are on the topic of making up fixatives I have a
supplementary question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a
glass rod. This is nerve-wracking, tedious (they do not
break readily) and not as safe a procedure as I would like.

The ampoules used to have a weakened neck which could be
sawn off but they do not come in this form from our
supplier any more.

Is there an easier way?

Dave


On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
} with a Teflon lined screw cap. The bottle is then held in the refrigerator
} in a small plastic jar (available from all lab distributors - mine from
} Fisher or S/P) with a screw cap . In this way, the plastic becomes
} osmicated NOT the refrigerator. I have done this for years with no untoward
} results.
}
} I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass or
} plastic. Osmication is done in glass vials with Teflon lined screw caps
} using a minimum of OsO4 to do the job.
}
} I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
} HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
} Teflon lined cap. I then make up an appropriate amount of fixative using
} the 10% glu and 10X buffer concentrate where possible. The 10% Glu is kept
} in a plastic jar in the refrigerator.
}
} I find that double containers are safe and reduce contamination
} considerably.
}
} Hope this helps.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"





From daemon Fri Sep 7 16:02:46 2001



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Fri, 7 Sep 2001 15:54:34 -0500
Subject: Thanks for tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who responded to this question. The most often-cited equation
was:

Real length = Observed length/cosine (tilt angle)

Several people noted that sample geometry plays a role, and that this
equation is useful only for flat samples.

Have a good weekend, everyone.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Fri Sep 7 16:10:39 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 7 Sep 2001 17:05:33 -0400
Subject: RE: Bio. Fixation: Which Champy's?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Gray, Peter, 1975, Microtomist's Formulary and Guide, R. Krieger,
Huntington, NY. (Still available for the hardy bibliophile at
http://WWW.WEB4U.COM/cgi-bin/full_page?0-88275-247-2) reports FOUR fixative
mixtures that are attributed directly to Champy:

1. Sat. aq. phenol(200ml)+40%HCHO(48ml)+Trichloroacetic
acid(3.6g)

2. HOH(20)+2% CrO3(50)+pyrolignious acid (35)(URL:
http://www.ayrshirehistory.org.uk/AcidWorks/acidworks.htm#Pyroligneous%20Aci
d)

3. HOH(33)+2% OsO4(22)+2%CrO3(20)+7.5% K2Cr2O7(16)

4. HOH(60)+7.5% K2Cr2O7(40)

My favorite, of course, is number 2!!! whose last component provides a
wonderful bit of chemical history. We see how Bayer must have started.
Dupont.

Regards, and I know that this doesn't help at all!

But it is all part of the art.

Fred Monson









} ----------
} From: Richard Edelmann
} Reply To: edelmare-at-muohio.edu
} Sent: Wednesday, September 5, 2001 4:24 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Bio. Fixation: What is Champy's Fluid?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} "Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed
} by
} ETOH dehydration, CPD, mounting and SEM viewing.
}
}
} O.k., we give up, searched numberous books, papers, catalogs, web
} sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
} "Champy's Solution"? Please?
}
} Thank you much!
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}


From daemon Fri Sep 7 16:37:45 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Fri, 07 Sep 2001 17:35:51 -0400
Subject: Re: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


EMS sells an ampoule breaker , Cat# 60600 for $12. We've used them for
breaking ampoules of paraformaldehyde, glutaraldehyde, acrolein and osmium
within seconds. The soft silicon molds can be twisted to achieve a quick
break. I usually tap the osmium tetroxide crystals to the bottom and use
forceps to lift the open ampoule into my prepared 50 ml of distilled water
to make our stock 2%. I have no financial interest in this company but am
a satisfied customer for many years. Phone number is: (800) 523-5874.
Rosemary Walsh,
EMF for the Life Sciences
PSU



From daemon Fri Sep 7 16:43:16 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 7 Sep 2001 16:39:49 -0500
Subject: Wiggl vs. waggle

Contents Retrieved from Microscopy Listserver Archives
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Fred:
Have you given any though to the fact that Coriolis' forces are
different in the southern hemispher? Water drains from a basin in a
counterclockwise motion south of the Equator and clockwise north of the
Equator. Maybe wiggles and waggles are affected thesame way between the
eastern and western hemispheres.

Regards,

Sam Purdy



Technical Center
National Steel Corp.
1745 Fritz Dr.
Trenton MI
Voice:734-676-2682
FAX: 734-676-2682
spurdy-at-nationalsteel.com


From daemon Fri Sep 7 17:28:59 2001



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Fri, 7 Sep 2001 18:25:23 -0400
Subject: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Use a triangular metal file to score around the neck, then use the
usual precautions (protect fingers, open in hood , etc.) to pop open the
vial. The EMS brand I use breaks open easily by wrapping paper towel/ gauze
around top and gentle application of pressure.


Becky Garrison
I am not an employee of any EM supplier.

-----Original Message-----
} From: Patton, David [mailto:David.Patton-at-uwe.ac.uk]
Sent: Friday, September 07, 2001 5:31 AM
To: Monson, Frederick C.
Cc: 'Microscopy Listserver'


While we are on the topic of making up fixatives I have a
supplementary question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a
glass rod. This is nerve-wracking, tedious (they do not
break readily) and not as safe a procedure as I would like.

The ampoules used to have a weakened neck which could be
sawn off but they do not come in this form from our
supplier any more.

Is there an easier way?

Dave


On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
} with a Teflon lined screw cap. The bottle is then held in the
refrigerator
} in a small plastic jar (available from all lab distributors - mine from
} Fisher or S/P) with a screw cap . In this way, the plastic becomes
} osmicated NOT the refrigerator. I have done this for years with no
untoward
} results.
}
} I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass
or
} plastic. Osmication is done in glass vials with Teflon lined screw caps
} using a minimum of OsO4 to do the job.
}
} I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
} HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
} Teflon lined cap. I then make up an appropriate amount of fixative using
} the 10% glu and 10X buffer concentrate where possible. The 10% Glu is
kept
} in a plastic jar in the refrigerator.
}
} I find that double containers are safe and reduce contamination
} considerably.
}
} Hope this helps.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Sep 7 18:10:29 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 07 Sep 2001 19:04:54 -0500
Subject: Breaking osmium tetroxide ampoules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

David Patton wrote:
========================================================
While we are on the topic of making up fixatives I have a supplementary
question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a glass rod.
This is nerve-wracking, tedious (they do not break readily) and not as safe
a procedure as I would like.

The ampoules used to have a weakened neck which could be sawn off but they
do not come in this form from our supplier any more.

Is there an easier way?
==========================================================
I am a bit surprised to hear that you are getting osmium tetroxide that is
not in prescored ampoules. So far as I know, all of the major suppliers of
EM chemicals, like SPI, have long since supplied their ampoules in the pre-
scored form. The only time I have seen in recent years ampoules not in pre-
scored form are those that are destined for other markets and applications.

Also, SPI, as well as the other major suppliers have long offered ampoule
neck breakers, such as are described on URL
http://www.2spi.com/catalog/tools/smtol21.html
These are meant to be used one time and discarded. They are inexpensive and
seem to provide an extra element of safety.

Using only prescored ampoules and an ampoule neck breaker might be easier on
the nerves.....

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sat Sep 8 11:38:40 2001



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Tuesday, September 04, 2001 4:09 AM
Subject: TEM CM10 Help!

Contents Retrieved from Microscopy Listserver Archives
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Marco,

Below are the two answers to your question, since I am not sure whether you
have
obj. aperture inserted in the beam, or you don't. Both assuming that the
field of view that you mentioning is the image of the sample in the holder,
rather than an illumination circle.

1. If obj. apertures holder is inserted in the beam.
Objective aperture should not be used in LM range. In Philips TEMs starting
with EM400 series , the main optical configuration difference between LM and
M modes is very distinct and as followed: in LM mode, the objective lens
current is very low (few hundreds milliAmps, depending on HT set), and is
fixed- hence magnification of obj. lens is also low (between 1 and 10,
closer to 1- just a guess). Focusing in LM mode is done by diffraction lens.
Almost all magnification is accomplished by lenses below the objective lens.
Obj. aperture is located between the specimen and that lens system, much
closer to the specimen, than to the first magnifying (diffraction) lens, and
is blocking most of the image if inserted in the beam.

In M mode, the obj. lens current is much higher (several Amps, depending on
the particular TEM model and the HT set), making magnification of the obj.
lens in M mode much higher (say, 50 or so). You can use obj. aperture now,
because of the significant part of the magnification happens very close to
the obj. aperture, plus much smaller area of the specimen is forming first
(objective lens) image. Focusing in M mode is done by the obj. lens.
Diffraction lens current in M mode is fixed and depends on the HT and
magnification set.

This two modes are "toggled" when TEM magnification is switched LM to M and
vice versa, in a single magnification step.

2. If obj. apertures holder is withdrawn from the beam.
When the condenser aperture is out of the beam, field of view in the lower
LM range increases. Nevertheless, obj. aperture holder shall not be visible
when withdrawn from the beam (control lever to the right). If obj. apertures
holder is still visible, then either the column alignment, or the obj.
aperture mechanical alignment (or both...) are incorrect.

If you are referring to illumination circle with the specimen holder
withdrawn- I wouldn't care much about obj. aperture holder visible on the
periphery of the illumination circle at the lowest LM steps, as long as it
is not blocking the sample image at the corresponding extreme of the sample
travel, and as long as everything else looks normal.

If you need to align the "out" position of any aperture holder on this TEM,
turn little Allen set screw located on the right side of the appropriate
aperture holder mechanism housing.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: "marienti-at-tiscalinet.it"-at-sparc5.microscopy.com
{"marienti-at-tiscalinet.it"-at-sparc5.microscopy.com}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}




From daemon Sun Sep 9 18:56:36 2001



From: Lyn Waterhouse :      lyn.waterhouse-at-adelaide.edu.au
Date: Mon, 10 Sep 2001 09:09:57 +0930
Subject: Australian 2002 EM Conference

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,

How would you like to escape the northern winter this year?

Yes? Then join us for the 17th Australian Conference on Electron
Microscopy, to be held in Adelaide, South Australia, from February 4 -
8, 2002. Encompassing the areas of Electron, Scanned Probe and Confocal
Microscopies, this important meeting will provide an opportunity to
explore the many applications of these techniques, to keep abreast of
the latest developments and to meet and mix with many of Australia's and
the world's eminent leaders in the field.
This meeting was last held in Adelaide in 1988. A lot has changed in
this city in the past 14 years. Adelaide has emerged as a vital city
which can offer the visitor a range of exciting opportunities. The local
wine and food have attained international recognition. Tourists are
welcomed and well catered for. The conference will be held in the brand
new Adelaide Convention Centre which is conveniently located in the CBD,
close to accommodation and other facilities.
International visitors should take some extra time either before or
after the conference to explore Adelaide and South Australia. Tours can
be arranged to take you to the sights of Adelaide, the nearby
picturesque Adelaide Hills, and the wine growing regions of McLaren
Vale, the Barossa Valley and Clare Valley. Each of these areas has its
own unique history, heritage sites and gourmet delights! Adelaide's
clean white beaches are within easy reach of the city. Experience the
wildlife, flora and landscape of Kangaroo Island. Adelaide is also the
perfect stepping-off point to locations such as the beautiful Flinders
Ranges, where centuries-old Aboriginal art and ancient geological
formations can be seen, Uluru, the opal mining town of Coober Pedy, and
the Outback. The current exchange rates ensure that travel in Australia
has never been more economical for the overseas tourist.
A series of pre-conference workshops will be held to allow participants
to gain hands-on experience in the latest techniques and applications.
Many of the keynote speakers will participate as presenters, providing a
wonderful opportunity for interaction with these noted scientists. Be
sure to book early, as places in some of the workshops is limited.
During the week, delegates will have the chance to mingle and relax
after hours in several of Adelaide's noted heritage locations. A range
of accommodation choices, from five-star to budget, ensures choices
suitable for all.
With the deadline for abstract submission fast approaching, (October 6),
start making plans now to join us in February. Abstracts should be
submitted electronically, and the registration form can be downloaded
from the website. Full details can be obtained at
http://www.adelaide.edu.au/CEMMSA/acem17 Keep an eye on the website for
regular updates.
We look forward to seeing you at ACEM17.

ACEM17 Organising Committee


CEMMSA
Centre for Electron Microscopy and Microstructure Analysis
Adelaide University
Adelaide SA 5005
Ph: (08) 8303 4074
Fax: (08) 8303 4356
Website: http://www.adelaide.edu.au/CEMMSA


From daemon Sun Sep 9 19:03:01 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 10 Sep 2001 10:04:48 +1000
Subject: Re: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
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At 10:30 7/09/01 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


we 1.
thouroughly clean off any label and label glue from the vial

2. put a scratch right around the vial

3. THEN it becomes easier to smash in the bottle, as you do it.

AND if you want, you can promote a crack around the line of the scratch by
touching a redhot glass rod to the scratch.

AND if you want, you can distribute the OsO4 in a thinner layer around the
wall of the vial by warming the (unscratched) vial in hot water, then
rolling the vial as the OsO4 cools.

This accelerates solution.......

AND if your OsO4 starts to go dark, you can refresh it almost completely by
adding hydrogen peroxide dropwise and thus titrate it to a clear solution
again. Oten you can restore it to straw colour, but we will use even a
pale purple solution as it will have enough tetroxide in it to work.






From daemon Sun Sep 9 20:13:36 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Sun, 9 Sep 2001 21:11:32 -0400
Subject: Re: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Upon reading subsequent posts about breaking OsO4 vials, I realized I must
amend my suggestion of wrapping the neck with paper towels and gently
applying pressure to break at the neck. This method works fine on
PRE-SCORED vials, but almost certainly is unsafe or ineffective when used
on unscored vials. I wasn't aware that unscored vials were still being
sold.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Sun Sep 9 20:56:06 2001



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Mon, 10 Sep 2001 11:55:32 +1000
Subject: Re: Electron Microscopes: Electric Trains, Buses, Subway

Contents Retrieved from Microscopy Listserver Archives
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Hi John,

I have an Hitachi H7100FA TEM on the second floor of a building built
almost directly over an underground electric railway. When I moved
in, bringing the EM with me, there was the choice of various
locations. I spent very little time standing on a wooden floor
feeling it shake under me. I spent considerably more time sitting on
a concrete floor with a saucer of water watching for vibrations (we
had no money to pay experts). Apart from having someone ask me if I
needed medical help, I was left in peace with my saucer and am now
quite happy with the chosen location. I don't work at REALLY high
mags, but the resolution test was accomplished without any vibrations
and I've coexisted happily with the trains for 3 years. Before that I
was on the 2nd floor of a concrete building next to a busy road and
it was dreadful. The building construction and location within it was
the important aspect for me.

Diana

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Mon Sep 10 00:56:38 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 10 Sep 2001 15:55:51 +1000
Subject: Oxford ISIS computer

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

We are currently running an Oxford ISIS EDS system and an Oxford MonoCL
cathodoluminescence system from the same computer and on the same SEM. We
want to split the Cathodoluminescence system off onto another column so we
need another ISIS board enclosure (box).

We would be pleased to hear from anyone upgrading their system who would
have an ISIS box that is spare. (The box that houses the ISIS boards, not
the PC.) We do not specially need the ISIS boards.

We will pay a reasonable amount (say US$5,000) plus shipping for an ISIS
box in working order.




Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400
http://srv.emunit.unsw.edu.au/


From daemon Mon Sep 10 02:10:34 2001



From: Alistair Smith :      A.Smith-at-macaulay.ac.uk
Date: Mon, 10 Sep 2001 08:06:21 +0100
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Alistair Smith
Head of Analytical Group
The Macaulay Institute
Craigiebuckler
Aberdeen AB15 8QH
Scotland UK

Tel: +44(0) 1224 498228
Fax: +44(0) 1224 498208

e-mail: a.smith-at-macaulay.ac.uk

Web sites:
http://www.macaulay.ac.uk
http://www.macaulay.ac.uk/analytc.htm




From daemon Mon Sep 10 06:25:39 2001



From: Erasmus, Willem (WJ) :      willem.erasmus-at-sasol.com
Date: Mon, 10 Sep 2001 13:16:15 +0200
Subject: Epoxy question

Contents Retrieved from Microscopy Listserver Archives
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Dear microscopists

I'd like to prepare catalyst particles for TEM viewing by ultramicrotomy.
The problem is that I want to study the location and nature of carbon in
these particles and I am not certain how to distinguish the carbon from the
epoxy. We currently use Spurr's resin, but is there an epoxy I can use that
is not carbon based ?

TIA
Willem Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]




From daemon Mon Sep 10 07:11:36 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Mon, 10 Sep 2001 22:05:26 +1000
Subject: Fw: Carnoy 1.0: scientific imaging for Mac OS X and Windows

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Could be interesting for you...
----- Original Message -----
} From: "Steven Dessein" {steven.dessein-at-BIO.KULEUVEN.AC.BE}
Dear Taxacomers,

The Laboratory of Plant Systematics (K.U.Leuven, Belgium) today released
Carnoy. Carnoy is an easy-to-use image analysis program, designed to carry
out measurements on digital images and especially on micrographs obtained
from light or electron microscopes.

Carnoy features a unique one-click calibration option: click on the scale
bar, enter its length and you are ready to carry out real-world measurements
on your micrographs.
Carnoy measures length and surface area of regular and irregular objects. It
also performs automated particle analysis (counting and measuring). The
program can handle BMP, PICT, Photoshop, JPEG, GIF, PNG, SGI, TGA, TIFF and
QuickTime image files and can convert between these file types.
Measurements are stored in a list that smartly manages all data. Every
measurement can be annotated. Measurements can be exported to a
tab-delimited file, which can be read by any text editor, word processor,
database or spreadsheet, for further analysis.
The program's clean and easy-to-use interface enables you to be productive
within minutes.

Carnoy can be used in the following fields: taxonomy, morphology, ecology,
physiology, molecular cell biology, agricultural sciences, paleontology,
archaeology, among others.
Carnoy requires a Macintosh running Mac OS X or a PC with at least 64 MB RAM
and Windows 95 / 98 / ME / NT / 2000 / XP. A resolution of 1024 x 768 pixels
(or higher) is recommended.

Carnoy is $15 shareware (if you cannot afford this, please, contact
peter.schols-at-bio.kuleuven.ac.be). It is available for download on the Carnoy
website.
http://www.carnoy.org

Kind regards,


P. Schols, S. Dessein & E. Smets


Steven Dessein
Laboratory of Plant Systematics
Institute of Botany and Microbiology
Kardinaal Mercierlaan 92
B-3001 Leuven
Belgium

tel. ++ 32 16 321536
fax. ++ 32 16 321968

http://www.kuleuven.ac.be/bio/sys
http://www.kuleuven.ac.be/bio/sys/mactaxon

e-mail: steven.dessein-at-bio.kuleuven.ac.be




From daemon Mon Sep 10 07:42:24 2001



From: pmoore-at-wfubmc.edu (Paula Moore)
Date: Mon, 10 Sep 2001 08:33:25 -0400
Subject: Re: Breaking osmium tetroxide ampoules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Folks,

I'm not sure if our Osmium vials are prescored or not, but we've never had any
problems or the nerve wracking experiences that you have mentioned.
We score around the vial a couple of times with a diamond pen(made especially
for marking on glass). The side where we want the break is scored a couple
times more. In the fume hood we wrap the vial in a paper towel and snap it
facing toward the back of the hood.
Hope this helps.

Paula Moore
Wake Forest University School of Medicine
Pathology/EM Lab
pmoore-at-wfubmc.edu


}
} How do you open glass osmium tetroxide ampoules?
}
} I put mine in a 100ml glass bottle and then break it with a glass rod.
} This is nerve-wracking, tedious (they do not break readily) and not as safe
} a procedure as I would like.
}
} The ampoules used to have a weakened neck which could be sawn off but they
} do not come in this form from our supplier any more.
}
} Is there an easier way?
} ==========================================================
}
}
} Using only prescored ampoules and an ampoule neck breaker might be easier on
} the nerves.....
}
} Chuck
}
} ==================================================



From daemon Mon Sep 10 08:56:17 2001



From: Melissa Troost :      m-troost-at-northwestern.edu
Date: Mon, 10 Sep 2001 08:49:20 -0500
Subject: SEM available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Amray 1610 SEM with PGT EDS free to a good home---interested party pays all
shipping charges. Please send queries off-list.
Thanks!

Melissa Troost
Electron Probe Instrumentation Center
Northwestern University
Evanston, Illinois, USA
847-491-3439
m-troost-at-northwestern.edu


________________________________
Legal Notice: This message is confidential is intended for the stated
addressee(s) only.
Access to this e-mail by anyone else is unauthorized. If you are not the
intended recipient, please inform the sender immediately.



From daemon Mon Sep 10 08:58:53 2001



From: Mark Germani :      mgermani-at-micromaterialsresearch.com
Date: Mon, 10 Sep 2001 08:56:39 -0500
Subject: SEM photos of microbiologicals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Does anyone have or know of a source of SEM photomicrographs of
bacteria, mold and yeast. I am asking on behalf of a colleague who will
pay a royalty for the use of the photos.


Mark Germani, Ph.D.
MicroMaterials Research, Inc.
136 Shore Drive, #200
Burr Ridge, IL 60521

(630) 325-8170
(630) 325-8178 fax

mgermani-at-micromaterialsresearch.com




From daemon Mon Sep 10 12:26:26 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 10 Sep 2001 13:17:49 -0400
Subject: Re: TEM of x-ray film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html






Anyone out there knows how to run up an X-ray film (exposed as well as
unexposed) for TEM?????


Dear Neelima,
What do you mean by "run up"? We are lucky to have our LoDose and
MRF32 x-ray films cut to the appropriate size--6.5 x 9 cm--and we treat
them like other EM film EXCEPT, we load and develop them in total darkness,
and we use x-ray film developer in the tank instead of D-19. Our darkroom
is in the same room as the scope, so transport can occur in total darkness.
If this is not the case for your facility, and you have to run the film up
to your darkroom, get a film transport bag from a photo supply store, put
the film in its original box, put the box in the bag, and carry it to your
darkroom. The bags are designed to be light tight. Good luck, and if you
mean something else by "run up", let the list know, and I'll try to give
you a better answer.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Mon Sep 10 13:51:57 2001



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Mon, 10 Sep 2001 15:03:42 -0400
Subject: RE: Ethanol vs. Acetone for critical point drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Karen:
Stick to absolute ethyl alcohol (Et0H). Never use acetone with any critical
point drying apparatus that have glass viewing windows held in place with a
epoxy resin that would be attacked by acetone.
The white crystals may very well be buffer salts. You can start dehydrating
with a larger ratio of water to ethyl alcohol and let stand for a longer
time to remove buffer salts while working up to absolute strength ethanol.
For some samples such as tissue culture monolayers the critical point
dryers can be avoided altogether by proceeding from the absolute ethyl
alcohol to 1,1,1,3,3,3-hexamethyldisilazane to air dry. I would recommend
the 99.9% pure solution.

Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
State University of New York-Binghamton
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu

} ---Original Message------------------
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."



From daemon Mon Sep 10 14:49:28 2001



From: yan.hu :      yan.hu-at-brunel.ac.uk
Date: Tue, 11 Sep 2001 02:35:37 -0500
Subject: ask help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I got a problem when preparing TEM foils of Al-alloy by means of
electro-polishing. A dark film produced on the specimen surface when it
was been polishing. I think, the polishing condition is right and
listed below:

Solution, 25 % Nitric acid plus 75 % Mechenol
Temperature, about -30 degree C
Voltage, 15-20V

I would be pleased to hear from anyone who has an idea to sort out this
problem.


Regards

Yan
----------------------

yan.hu-at-brunel.ac.uk


From daemon Mon Sep 10 14:49:30 2001



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Tue, 11 Sep 2001 02:36:21 -0500
Subject: Re: Drosophila brain fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Randy,
Drosophila brains are very hard to fix. The neurons are very
delicate and lose their structure when not well preserved. What age
are the bugs? You might try some adding some acrolein (we used 1%
acrolein and 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.1)
and leave out the paraformaldehyde. See if you still have
immuno-reactivity You don't need to use propylene oxide. You can
use 100% EtOH instead. It is miscible with resins, as long as it is
totally dry. Also, don't store your specimens in buffer, but process
straight through. I do microwaving these days, so time is never an
issue anymore. Good luck!


JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856

04:18 PM 09/06/2001 -0500, you wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Mon Sep 10 16:29:20 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Mon, 10 Sep 2001 14:20:25 -0700
Subject: Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


In conjunction with the Pacific Northwest Microscopy Society,
The University of Washington Core for Communication Research, Center on
Human Development and Disability and the W. M. Keck Center for Advanced
Studies in Neural Signaling present:

Microscopy and Digital Imaging: Advances & Applications

September 27, 2001
12:30 - 5 p.m.
Location: CD 150

12:30 "Multiphoton and Multispectral Imaging in Vertebrates"
Mary Dickinson, Caltech

1:15 "Some New Approaches to Intracellular Imaging"
Bob Fern, University of Washington

2:00 FRET imaging with the confocal microscope
Kolja Wawrowsky, Leica

2:45 Break

3:15 "In Vivo Microscopy of Murine Brain"
Liz Yoder, UCSD

4:00 "Deconvolution Methods in Wide-field and Confocal Microscopy"
Paul Goodwin, Applied Precision Inc

5:00 Reception

The Veteran's Administration Hospital and Medical Center Morphological
Analysis Core Facility will have an open house and talks on September
28, 2001.

Speakers sponsored by Applied Precision, BioRad, Leica, and Zeiss
The reception is sponsored by Bartels and Stout, Inc.

Contact Paulette Brunner pbrunner-at-u.washington.edu or Glen MacDonald
glenmac-at-u.washington.edu for information.

Regards,
Glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************

C:} The box said "Requires Windows95 or better". So I bought a
Macintosh.
**************************************************************************





From daemon Mon Sep 10 17:27:38 2001



From: sbcook-at-bcpl.net ()
Date: Tue, 11 Sep 2001 05:22:19 -0500
Subject: Ask-A-Microscopist:Germs and Science Projects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sbcook-at-bcpl.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
September 9, 2001 at 22:52:34
---------------------------------------------------------------------------

Email: sbcook-at-bcpl.net
Name: Katelyn Cook

Organization: St. Michael the Archangel

Education: 6-8th Grade Middle School

Location: Baltimore, MD

Question: I am planning to do a science fair project about germs and
the importance of hand washing. I want to collect germs from common
public places such as doorknobs, handrails, telephones, sink faucets,
etc. What is the best way to collect the germs? Will I need to grow
the germs in a petri dish after I collect them? Will I be able to
identify different types of germs using my microscope?

---------------------------------------------------------------------------


From daemon Mon Sep 10 17:40:42 2001



From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Mon, 10 Sep 2001 15:35:20 -0700
Subject: Re: Drosophila brain fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have seen cellular voids from poor fixation as well as from genetic
mutations. The biggest impediment I have when fixing drosophila brains is
getting the fixative to the tissue. I recommend cutting the neck as well as
the proboscis and then using gentle centrifugation (microcentrifuge
~2000rpm for one minute) to get the heads to sink. Due to air in the brain
cavity this last step does not always work. If you use 2% glutaraldehyde
and osmium post fixation do you get good ultrastructure? In a project I did
some time ago I had the best results using 0.07 M sodium cacodylate buffer
at pH 7.5. That gives an osmolarity of about 187mOsm. However, I routinely
use 0.1 M sodium cacodylate and find that adequate.

At 04:18 PM 9/6/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Sep 10 18:15:45 2001



From: Ubirajara Pereira Rodrigues Filho :      uprf-at-iqsc.sc.usp.br
Date: Mon, 10 Sep 2001 20:11:26 -0300
Subject: SEM of tempers in potteries ( specially shells )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Liststers;
}
} I'm working with SEM of pre-colonial potteries from Pantanal at the =
} Brazilian West border with Bolivia. In some samples we've found shell =
} and sponge spicule in the bulk of potteries. We would appreciate any =
} article or book with micrographies of similar materials in pottery ? =
} Also, if someone could provide me references on clay with sponge =
} spicule, would be very helpful.
}
} Ubirajara Pereira Rodrigues-Filho
Instituto de Química de São Carlos
Universidade de São Paulo
São Carlos, Brazil



From daemon Mon Sep 10 18:40:16 2001



From: Corvos-at-aol.com
Date: Mon, 10 Sep 2001 18:34:50 -0500
Subject: Re: Cameca MBX Microprobe is available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


All,

Cameca MBX Microprobe is available at this time.
4 wavelength spectrometers (includes PC2 crystal for Lt ele. spec.)
Kevex Sigma EDS
New GW Backscattered system
PC Based operating system
Diff pump vacuum system
Cathodoluminescence system (OEM)
System is about 17 years old and in good condition (at last service call).
Has been turned off for 1 year.

Please send inquires to E-MAC
Walter Protheroe
corvos-at-aol.com

All inquires will be passed along.


From daemon Mon Sep 10 18:47:36 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Mon, 10 Sep 2001 16:39:59 -0700
Subject: Re: SEM photos of microbiologicals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Mark:

You may want to try the following website at Utah State University:

http://bioweb.usu.edu/emlab/Galleries.html

They have many great SEM images. A contact person there to talk to
would
be:

Bill McManus
TEL: (435) 797-1920

I hope this helps.

Best regards-

David

Mark Germani wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Does anyone have or know of a source of SEM photomicrographs of
} bacteria, mold and yeast. I am asking on behalf of a colleague who will
} pay a royalty for the use of the photos.
}
} Mark Germani, Ph.D.
} MicroMaterials Research, Inc.
} 136 Shore Drive, #200
} Burr Ridge, IL 60521
}
} (630) 325-8170
} (630) 325-8178 fax
}
} mgermani-at-micromaterialsresearch.com

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Mon Sep 10 18:55:52 2001



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Mon, 10 Sep 2001 18:50:17 -0500
Subject: Re: Drosophila brain fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Randy,
Drosophila brains are very hard to fix. The neurons are very
delicate and lose their structure when not well preserved. What age
are the bugs? You might try some adding some acrolein (we used 1%
acrolein and 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.1)
and leave out the paraformaldehyde. See if you still have
immuno-reactivity You don't need to use propylene oxide. You can
use 100% EtOH instead. It is miscible with resins, as long as it is
totally dry. Also, don't store your specimens in buffer, but process
straight through. I do microwaving these days, so time is never an
issue anymore. Good luck!


JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856

04:18 PM 09/06/2001 -0500, you wrote:

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello everyone,
I have a researcher here that is having some difficulty with getting good
histology at the EM level. She is fixing Drosophlia brains with 4%para/0.5%
glut in phosphate buffer pH7.2, no osmium post fix, ethanol dehaydration,
propylene oxide as a transitional solvent to polybed 812, then performing post
sectioning immunolabeling. The tissue has "holes" in it when viewed at the
TEM. They are not holes in the section, but appear to be voids in the tissue.
We have tried leaving out the propylene oxide, we have osmicated, and have
looked at osmotic strength of the fixative (and many variations of the above,
to no avail). Can anyone offer advice?
Randy Nessler
Univ. of Iowa


From daemon Mon Sep 10 20:27:05 2001



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Mon, 10 Sep 2001 19:16:43 -0600
Subject: RE: ask help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Yan,
Assuming (sorry for that) that the polishing conditions are correct, and you
are producing well dished samples with thin area and the only problem is
sludge on the surface, try two things before changing any other conditions
and try them one at a time so as not to confuse the issue.
1: Try increasing the jet speed. If this doesn't work.
2: Try increasing the temperature about 5 degs.
It would be helpful to know what type of jet thinner you are using and
exactly what the alloy is.
Good Luck!
Dorrance

} ----------
} From: yan.hu
} Sent: Tuesday, September 11, 2001 12:35 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: ask help
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} I got a problem when preparing TEM foils of Al-alloy by means of
} electro-polishing. A dark film produced on the specimen surface when it
} was been polishing. I think, the polishing condition is right and
} listed below:
}
} Solution, 25 % Nitric acid plus 75 % Mechenol
} Temperature, about -30 degree C
} Voltage, 15-20V
}
} I would be pleased to hear from anyone who has an idea to sort out this
} problem.
}
}
} Regards
}
} Yan
} ----------------------
}
} yan.hu-at-brunel.ac.uk
}
}
}



From daemon Tue Sep 11 00:14:03 2001



From: Nelson Conti :      NelsonC51-at-excite.com
Date: Mon, 10 Sep 2001 22:07:06 -0700 (PDT)
Subject: Re: Ask-A-Microscopist:Germs and Science Projects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



On Tue, 11 Sep 2001 05:22:19 -0500, sbcook-at-bcpl.net wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Below is the result of your feedback form (NJZFM-ultra-55). It was
| submitted by (sbcook-at-bcpl.net) from
| http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
| September 9, 2001 at 22:52:34
|
---------------------------------------------------------------------------
|
| Email: sbcook-at-bcpl.net
| Name: Katelyn Cook
|
| Organization: St. Michael the Archangel
|
| Education: 6-8th Grade Middle School
|
| Location: Baltimore, MD
|
| Question: I am planning to do a science fair project about germs and
| the importance of hand washing. I want to collect germs from common
| public places such as doorknobs, handrails, telephones, sink faucets,
| etc. What is the best way to collect the germs? Will I need to grow
| the germs in a petri dish after I collect them? Will I be able to
| identify different types of germs using my microscope?
|
|
---------------------------------------------------------------------------
|

Dear Katelyn Cook -
While I was teaching as a graduate teaching assistant for a general
biology class, I had the opportunity to lead a group of non-
science college students on an examination of the microbial world around
them. What I did was to have each of them use a sterile, cotton-
tipped swab, moisten it a bit with sterile water (perhaps boiled water will
do just as well), swab the surface of whatever object they wanted to
explore, and then swab it onto the surface of a petri plate that already had
bacterial medium. They then left all the plates for me to seal and
incubate at room temperature for several days (or, at most, 1 week). When
they got the plates back, they then could see with the naked eye
the various colonies that formed and their various colors. If a microscope
is available, then it is possible to identify individual bacteria by using
a "wet mount" (that is, a slide with a small drop of sterile water and a
very small amount of whatever colony is chosen).
Although I don't have any experience with grade school students, I believe
that my approach could work. So... to answer your
questions: (1) A very easy way to collect bacteria is to first get some
sterile swabs. The fact that they are sterile is important because
any box of ordinary (or household) swabs is likely to already have bacteria
on them, and obviously that would defeat the purpose of your
experiment. As a result, you'll need some sterile swabs -- quite likely,
they can be bought at a pharmacy near you when you look for dressings,
bandages, etc. (2) Assuming you have sterile swabs, you could conceivably
boil some water, let it cool to room temperature, and let each
student lightly wet a swab (or more as needed). Why boil the water? For the
same reason as needing sterile swabs -- because ordinary tap
water will very likely contain bacteria (even if it looks perfectly clear).
One alternative is to use distilled water if boiling isn't feasible.
(3) Yes, you will need a petri plate -- the plate needs to have some kind of
bacterial medium capable of supporting their growth; otherwise,
your swabbing exercise will be fruitless. I do not know where such plates
can be obtained, though, and you may want to seek others on
this listserver; finally, (4) The use of a microscope is needed only if
you're interested in examining individual cells. The typical colonies that
are seen on the surface of a plate tend to vary in terms of both color and
the type of colony produced. That is, a colony could be white or
yellow, but its 'type" could be of a sticky nature or a more "liquid-like"
nature. That part could be revealing on its own, or perhaps not.
If you feel that a microscope is needed, then a simple way to see individual
cells is to simply add a small drop of boiled / sterile water onto a
slide, use an inoculating loop or needle, and gently pick up a small amount
of a colony, and spread it around within to the drop. Place a coverslip
over the slide, and put it onto the stage of a microscope. Try to use the
highest objective lens magnification that is possible -- if there are a 10X,
20X and
100X objectives, use the 20X as the individual cells are very small in size
-- and, if it's possible, try to set the microscope under non-brightfield
light conditions such as phase-contrast. Bacterial cells are, generally,
transparent to light, and as a result are often very hard to see because
they have very little contrast with the background light.
Anyway .. I hope you have some success with your experiment for these
grade-school students. It is my my belief that microscopic, living
things (such as the "germs" and, more specifically, bacteria, algae, etc.)
are always fascinating things for kids to see, and I hope it turns out
very well.
Good luck !
Nelson Conti
Former graduate student at San Francisco State University
(in San Francisco, CA)
A Bachelor's and Master's degree in Microbiology





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Tue Sep 11 02:13:28 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 11 Sep 2001 08:14:32 +0100 (GMT Daylight Time)
Subject: Re: Oxford ISIS computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Mel,

Have you contacted Oxford Instruments? With the INCA doing
as well as they tell me it is they must be taking in quite
a few ISIS towers on upgrades (they've had one of ours).
Of course if you expect a full manufacturer's warrantee it
might be pricey but if you took the same 'as seen'
conditions that you would accept from another user then
they might deal.

Regards,
Ron

On Mon, 10 Sep 2001 15:55:51 +1000 Mel Dickson
{m.dickson-at-unsw.edu.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} We are currently running an Oxford ISIS EDS system and an Oxford MonoCL
} cathodoluminescence system from the same computer and on the same SEM. We
} want to split the Cathodoluminescence system off onto another column so we
} need another ISIS board enclosure (box).
}
} We would be pleased to hear from anyone upgrading their system who would
} have an ISIS box that is spare. (The box that houses the ISIS boards, not
} the PC.) We do not specially need the ISIS boards.
}
} We will pay a reasonable amount (say US$5,000) plus shipping for an ISIS
} box in working order.
}
}
}
}
} Dr. Mel Dickson,
} Deputy Director, The Electron Microscope Unit,
} Adjunct Associate Professor, School of Microbiology & Immunology
} The University of New South Wales
} UNSW SYDNEY 2052
} Australia.
} Phone +612 9385 6383 Fax +612 9385 6400
} http://srv.emunit.unsw.edu.au/
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Sep 11 02:38:09 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Fri, 07 Sep 2001 17:35:51 -0400
Subject: Re: Question about preparing osmium tetroxide

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EMS sells an ampoule breaker , Cat# 60600 for $12. We've used them for
breaking ampoules of paraformaldehyde, glutaraldehyde, acrolein and osmium
within seconds. The soft silicon molds can be twisted to achieve a quick
break. I usually tap the osmium tetroxide crystals to the bottom and use
forceps to lift the open ampoule into my prepared 50 ml of distilled water
to make our stock 2%. I have no financial interest in this company but am
a satisfied customer for many years. Phone number is: (800) 523-5874.
Rosemary Walsh,
EMF for the Life Sciences
PSU




From daemon Tue Sep 11 02:38:24 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 07 Sep 2001 19:04:54 -0500
Subject: Breaking osmium tetroxide ampoules

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

David Patton wrote:
========================================================
While we are on the topic of making up fixatives I have a supplementary
question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a glass rod.
This is nerve-wracking, tedious (they do not break readily) and not as safe
a procedure as I would like.

The ampoules used to have a weakened neck which could be sawn off but they
do not come in this form from our supplier any more.

Is there an easier way?
==========================================================
I am a bit surprised to hear that you are getting osmium tetroxide that is
not in prescored ampoules. So far as I know, all of the major suppliers of
EM chemicals, like SPI, have long since supplied their ampoules in the pre-
scored form. The only time I have seen in recent years ampoules not in pre-
scored form are those that are destined for other markets and applications.

Also, SPI, as well as the other major suppliers have long offered ampoule
neck breakers, such as are described on URL
http://www.2spi.com/catalog/tools/smtol21.html
These are meant to be used one time and discarded. They are inexpensive and
seem to provide an extra element of safety.

Using only prescored ampoules and an ampoule neck breaker might be easier on
the nerves.....

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From daemon Tue Sep 11 02:38:26 2001



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Fri, 7 Sep 2001 18:25:23 -0400
Subject: Question about preparing osmium tetroxide

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Use a triangular metal file to score around the neck, then use the
usual precautions (protect fingers, open in hood , etc.) to pop open the
vial. The EMS brand I use breaks open easily by wrapping paper towel/ gauze
around top and gentle application of pressure.


Becky Garrison
I am not an employee of any EM supplier.

-----Original Message-----
} From: Patton, David [mailto:David.Patton-at-uwe.ac.uk]
Sent: Friday, September 07, 2001 5:31 AM
To: Monson, Frederick C.
Cc: 'Microscopy Listserver'


While we are on the topic of making up fixatives I have a
supplementary question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a
glass rod. This is nerve-wracking, tedious (they do not
break readily) and not as safe a procedure as I would like.

The ampoules used to have a weakened neck which could be
sawn off but they do not come in this form from our
supplier any more.

Is there an easier way?

Dave


On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
} with a Teflon lined screw cap. The bottle is then held in the
refrigerator
} in a small plastic jar (available from all lab distributors - mine from
} Fisher or S/P) with a screw cap . In this way, the plastic becomes
} osmicated NOT the refrigerator. I have done this for years with no
untoward
} results.
}
} I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass
or
} plastic. Osmication is done in glass vials with Teflon lined screw caps
} using a minimum of OsO4 to do the job.
}
} I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
} HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
} Teflon lined cap. I then make up an appropriate amount of fixative using
} the 10% glu and 10X buffer concentrate where possible. The 10% Glu is
kept
} in a plastic jar in the refrigerator.
}
} I find that double containers are safe and reduce contamination
} considerably.
}
} Hope this helps.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Tue Sep 11 02:38:26 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 7 Sep 2001 16:39:49 -0500
Subject: Wiggl vs. waggle

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Fred:
Have you given any though to the fact that Coriolis' forces are
different in the southern hemispher? Water drains from a basin in a
counterclockwise motion south of the Equator and clockwise north of the
Equator. Maybe wiggles and waggles are affected thesame way between the
eastern and western hemispheres.

Regards,

Sam Purdy



Technical Center
National Steel Corp.
1745 Fritz Dr.
Trenton MI
Voice:734-676-2682
FAX: 734-676-2682
spurdy-at-nationalsteel.com



From daemon Tue Sep 11 02:38:28 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Fri, 7 Sep 2001 14:45:06 -0400
Subject: Re: Ethanol vs. Acetone for critical point drying

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Karen:

Another source that we have come across is sodium phosphate buffer
crystals. If fixation is carried out with sodium phosphate buffered soluitons,
and then immediately transittioned to dehydration solvent (either EtOH or
Acetone) then we have seen samples coated with fine crystals. We use
either serveral water rinses between fixation and dehydration or an alternative
buffer (i.e. Na Cacodylate)

}
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."



From daemon Tue Sep 11 02:38:31 2001



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Fri, 7 Sep 2001 15:54:34 -0500
Subject: Thanks for tilt correction factor

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Thanks to all who responded to this question. The most often-cited equation
was:

Real length = Observed length/cosine (tilt angle)

Several people noted that sample geometry plays a role, and that this
equation is useful only for flat samples.

Have a good weekend, everyone.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com




From daemon Tue Sep 11 02:38:33 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 7 Sep 2001 17:05:33 -0400
Subject: RE: Bio. Fixation: Which Champy's?

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Gray, Peter, 1975, Microtomist's Formulary and Guide, R. Krieger,
Huntington, NY. (Still available for the hardy bibliophile at
http://WWW.WEB4U.COM/cgi-bin/full_page?0-88275-247-2) reports FOUR fixative
mixtures that are attributed directly to Champy:

1. Sat. aq. phenol(200ml)+40%HCHO(48ml)+Trichloroacetic
acid(3.6g)

2. HOH(20)+2% CrO3(50)+pyrolignious acid (35)(URL:
http://www.ayrshirehistory.org.uk/AcidWorks/acidworks.htm#Pyroligneous%20Aci
d)

3. HOH(33)+2% OsO4(22)+2%CrO3(20)+7.5% K2Cr2O7(16)

4. HOH(60)+7.5% K2Cr2O7(40)

My favorite, of course, is number 2!!! whose last component provides a
wonderful bit of chemical history. We see how Bayer must have started.
Dupont.

Regards, and I know that this doesn't help at all!

But it is all part of the art.

Fred Monson









} ----------
} From: Richard Edelmann
} Reply To: edelmare-at-muohio.edu
} Sent: Wednesday, September 5, 2001 4:24 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Bio. Fixation: What is Champy's Fluid?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} "Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed
} by
} ETOH dehydration, CPD, mounting and SEM viewing.
}
}
} O.k., we give up, searched numberous books, papers, catalogs, web
} sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
} "Champy's Solution"? Please?
}
} Thank you much!
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}



From daemon Tue Sep 11 02:45:27 2001



From: Macatangay, Peggy J., Celanese/US :      PJMckarns-at-celanese.com
Date: Thu, 23 Aug 2001 13:13:47 -0500
Subject: SEM-EDS: Help with choosing a new system

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I am investigating EDS systems to integrate with a JEOL-6100 SEM. I'm
leaning towards PGT's Spirit system. Other considerations are Oxford's INCA
and Thermo Noran's Vantage. Does anyone have particularly positive or
negative experience with PGT? I don't often hear much about that company.
Any comments about the other possibilities would be appreciated, as well.

Thanks!

-Peggy

Peggy McKarns Macatangay, PhD
Project Analyst 2
Celanese Corpus Christi Technical Center




From daemon Tue Sep 11 02:45:32 2001



From: john_bruss-at-bose.com ()
Date: Thu, 23 Aug 2001 13:45:37 -0500
Subject: Ask-A-Microscopist: how to suspend a spider in a clear cube

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (john_bruss-at-bose.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
August 22, 2001 at 17:00:52
---------------------------------------------------------------------------

Email: john_bruss-at-bose.com
Name: John Bruss

Organization: Bose Corp.

Education: Graduate College

Location: San Diego, CA, USA

Question: What materials and process would an amateur use to suspend
a spider in a clear cube for unmagnified artistic and historic use?
Where are those materials available in small quantities?

---------------------------------------------------------------------------



From daemon Tue Sep 11 02:46:21 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 23 Aug 2001 12:32:20 -0500
Subject: RE: Spectral Imaging with ISIS

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I am pretty sure that Alwyn is not talking about x-ray mapping. If that was
all he wanted, I suppose the key disk is all that would be needed if he
already has the other imaging applications and hardware installed.

Someone suggested using Auto to scan an image for multiple spectra. Indeed,
the software and hardware should allow it. The spectra might then be
exported and processed by some other package to perform the type of
component analysis that has been described at MSA over the last few years.
My current ISIS x-ray application (version 3.32) has the option of storing
the x-ray data in single column MSA format. Previous versions placed
multiple channels on a single line.

One hitch would be the limitation on the number of spectra per job. I think
there is a limit of 1000 or so built into the database used for managing
the data. I suppose it could be changed to another number, but we bumped
into it some time back.

That would mean you could do a 32x32 raster of points saving a spectrum at
each. A four second acquisition per point would mean a bit more than an
hour for acquisition. That would not be too bad, but the spatial resolution
would probably be too low to be very useful. But the data should readily
fit onto hard drives with capacities of a few gigabytes.

I would be interested in seeing how the data processing is handled. If
someone makes it work, I would be very interested in hearing the details.

Warren

At 10:33 AM 8/23/2001 -0400, you wrote:
} -----------------------------------------------------------------------.
} This is correct, the keydisk is all that is required for
} the software to be enabled. However, there may be hardware
} required, too, at least a cable interface (RS232?) to the
} microscope. I believe the ISIS Autobeam likes to select
} its own scan rates. You may need to speak with Oxford re:
} compatibility with your particular microscope.
}
} Matt
}
} Matthew J. Lynn
} Center for Advanced Microscopy
} University of Miami
} (305)284-4736
} mlynn-at-miami.edu
}
}
} On Wednesday, August 22, 2001 9:23 PM, Fred Pearson
} [SMTP:eoptics-at-mcmail.cis.mcmaster.ca] wrote:
} }
} } Alwyn:
} }
} } By spectral images, do you mean xray mapping? A "key" disk for the ISIS
} } software program is required to activate the xray mapping facility. Of,
} } course this will cost a bit of money to purchase from Oxford. When the
} } ISIS program is loaded initially, all the software required to use the
} } program is there, but activating the unpaid for parts of the program is
} } the thing.
} }
} } Fred
} }
} } On Wed, 22 Aug 2001, Alwyn Eades wrote:
} } } We have an Oxford ISIS EDS system. As it stands it is not set up to
} } } acquire spectral images. We would like to be able to do spectral image
} } } acquisition on this machine. Is there anyone out there who knows how
} } } the system may be modified (macros, interfaces, whatever) to do it? We
} } } do have Digital Micrograph (on a Mac whereas ISIS is on a PC), if that
} } } helps.
} } } ..........
} } } Alwyn Eades
} } } Department of Materials Science and Engineering
} } } Lehigh University
} } } 5 East Packer Avenue
} } } Bethlehem
} } } Pennsylvania 18015-3195
} } } Phone 610 758 4231
} } } Fax 610 758 4244
} } } jae5-at-lehigh.edu




From daemon Tue Sep 11 02:49:04 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 25 Aug 2001 15:57:15 -0400
Subject: Re: Which is Correct, EDS or EDX?

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Mike,
My guess is that either will do the trick, but I would take EDX as a
shortened version of Energy Dispersive Analysis of X-rays, which, of
course, is EDAX. I've also seen EDXA which would keep you out of
trouble with EDAX. One really is doing a specific type of analysis,
spectroscopy, which would cause me to lean towards Energy Dispersive
Spectroscopy.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ingram, Mike wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Not a serious question here, more of a curiosity.
}
} I use EDS when talking about x-ray analysis. I see many using EDX. Which
} is correct?
}
} Mike Ingram
}
}
}




From daemon Tue Sep 11 04:29:04 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Tue, 11 Sep 2001 10:20:48 +0000 (GMT)
Subject: Debye-Sherrer cameras

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Dear Listers,
In a clear out of a cupboard I have found a pair of Debye-Sherrer cameras and accessories. If anyone would like them, please contact me - otherwise they're skip fodder. I used to demo this as an undergraduate lab some years ago, but I guess no-one uses them any more?

Regards,

Richard Beanland

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and
confidential information intended for the eyes of the individual or
entity named above. If the reader of this message is not the
intended recipient, you are hereby notified that any dissemination,
distribution or copying of this message is strictly prohibited.
If you have received this message in error, please notify us
immediately by telephone."
Marconi Optical Components Limited
Registered in England No. 4113798 Registered Office: One Bruton Street London
W1J 6AQ





From daemon Tue Sep 11 07:03:32 2001



From: Wang, Dashan :      Dashan.Wang-at-nrc.ca
Date: Tue, 11 Sep 2001 07:56:14 -0400
Subject: looking for chips

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Dear Listers,

The CCD camera (model 679) in our CM20 TEM does not work any more. It is
the fist commercial CCD camera of Gatan. The company is experiencing hard
time to find the right replacing part. A programmable chip ( TEK1B2FV9
made by ACTEL) for the camera controller is needed. Then they can possibly
figure out what problems with the camera.

If you have this part or any information which can help us to sole this
problem please let me know. I can be reached at (613) 990-1403 or
dashan.wang-at-nrc.ca

Thank you!

Dashan Wang


From daemon Tue Sep 11 07:05:01 2001



From: hagglund.kw-at-pg.com
Date: Tue, 11 Sep 2001 07:58:19 -0400
Subject: Re: SEM of tempers in potteries ( specially shells )

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Try Prudence Rice's book Pottery Analysis: A Sourcebook (1987). This was the
text on archaeological ceramics a few years back, and would be a good starting
point. It is available for $65 on amazon.com.

Karl Hagglund
(513) 634-0146



From daemon Tue Sep 11 07:55:58 2001



From: Jill Verlander Reed :      verlaj-at-medicine.ufl.edu
Date: Tue, 11 Sep 2001 08:44:59 -0500
Subject: Re: Ask-A-Microscopist:Germs and Science Projects

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Dear Katelyn,
I will only add to Nelson Conti's remarks that you should be able to buy
"blood-agar plates," which are Petri dishes already filled with a
sterile, general bacterial culture medium, as well as sterile
cotton-tipped swabs, from a medical supply store in your area. My
daughter did a project along the same lines as yours and that's where we
got the supplies. The dishes came in packs of 10, I think and they only
cost a few dollars.
We kept the inoculated dishes in a warm place. Around body temperature
(37 degrees C, 98-99 degrees F) is ideal is you can find such a place.
If you find bacterial colonies that have a clear ring around them,
instead of the red of the rest of the blood agar, that is an indication
that the bacteria in that colony are pathogenic (disease-producing).
Good luck, and have fun.

Jill Verlander Reed, D.V.M.
Associate Scientist
Director, College of Medicine Electron Microscopy Core Facility
University of Florida
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299


From daemon Tue Sep 11 08:53:16 2001



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Tue, 11 Sep 2001 08:50:44 -0700
Subject: Re: Electron Microscopes: Electric Trains, Buses, Subway

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John

At UIC we are less than 800m from the Blue Line of the cta L. When the lab
was set up we bought an EMF cancellation system, in case. However, it
turned out we did not need it despite doing high resolution annular dark
field STEM with sub 0.2nm resolution.

While I was working for VG we did install a HB501 STEM in Halle,
Germany. Here it was a different story. The main streetcar line through
the city ran less than 100m from the site and to make matters worse a main
feed for the system ran perpendicular to the line along a second wall of
the building (probably 50m away). You could see each and every streetcar
entering the stretch of line powered by the feed, it caused a sideways
image jump! This was fixed by putting a Hall effect coil in the room at
microscope bench level with a feed into the alignment coils.

If this is a new transit system then you are likely to suffer less problems
with vibration and magnetic fields than you are with an old system where
maintenance and standards of construction are likely to be poorer.

Alan

At 12:05 PM 9/7/2001 -0700, John C. Wheatley wrote:
} ------------------------------------------------------------------------
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Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Tue Sep 11 09:05:51 2001



From: evgenia.pekarskaya-at-exxonmobil.com
Date: Tue, 11 Sep 2001 09:57:52 -0400
Subject: Re: ask help

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Yan,

Make sure that you withdraw the specimen from the polishing solution as
quickly as possible (!) and rinse immediately (!). I don't know what you
are using to rinse your specimens. If your electropolishing solution is
based on methanol, than it is a good idea to rinse it in methanol. I
normally rinse my specimens twice in two different beakers with methanol
or ethanol.
Good luck!

Evgenia

**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355




"yan.hu"
{yan.hu-at-brunel. To: microscopy-at-sparc5.microscopy.com
ac.uk} cc:
Subject: ask help

09/11/01 03:35
AM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers,

I got a problem when preparing TEM foils of Al-alloy by means of
electro-polishing. A dark film produced on the specimen surface when it
was been polishing. I think, the polishing condition is right and
listed below:

Solution, 25 % Nitric acid plus 75 % Mechenol
Temperature, about -30 degree C
Voltage, 15-20V

I would be pleased to hear from anyone who has an idea to sort out this
problem.


Regards

Yan
----------------------

yan.hu-at-brunel.ac.uk







From daemon Tue Sep 11 09:48:27 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 11 Sep 2001 09:46:46 -0500
Subject: Facility support survey redux

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Micro listers,

This is the second round. I have not received enough responses to
give any meaningful data. If you are interested in the results of
this survey, but have not responded for your facility, please do so.
Thanks!

This is a survey that was passed out at the Facility Managers Meeting
at this year's M&M meeting. We've only gotten 6 responses so far, and
many more are needed for valid results (although obviously only one
answer per facility). Please take a couple of minutes to fill this
form in, and email back to me. Thank you!

Phil

MICROSCOPY FACILITY SUPPORT

This survey will help compile the nature and amount of support
provided to microscopy facilities by universities, colleges, and
research institutes. We would like to determine how much facility
support is provided by the institution, and how much must be
generated by user fees or other sources, on a per cent basis.

If we get enough responses to have valid data, we will post the
results on the microscopy listserver and send them to the MSA
bulletin.
Names of institutions will not be included in the reported
results.

Institution:
Location:
Nature of institution (Academic, Commercial, private research, etc.)

Type of Facility (institution core, satellite, departmental, etc):

(Place an 'X' by the appropriate answer)
Predominately:
Biological
Materials
Both

User base:
Service
Multi-user
Both

Type of microscopes:
SEM
FESEM
ESEM or LV
TEM
FETEM
Major optical (Specify)
Scanning Probe (specify)
Other (specify)

Approximately what *per cent* of the funds for each of the following
is provided by
A) your institution, B) user fees, or C) grants

Salaries:
A)
B)
C)

Instrument Maintenance (Service contracts, instrument insurance, etc):
A)
B)
C)

Replacement of existing equipment:
A)
B)
C)

Purchase of new equipment (not replacement for but addition to
existing equipment):
A)
B)
C)

Supplies:
A)
B)
C)

Other operating expenses:
A)
B)
C)

Are there other microscope facilities at your institution? if
so, please indicate approximate number having electron
microscopes. Ideally we would like a form filled out for each
major facility (multiple instruments) at your institution if
there are more than one.

Please include additional information such as novel funding
ideas for your facility or for others at your institution.

Please email your responses to Philip Oshel
peoshel-at-facstaff.wisc.edu
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Tue Sep 11 10:21:12 2001



From: yan.hu :      yan.hu-at-brunel.ac.uk
Date: Tue, 11 Sep 2001 16:14:29 +0100 (BST)
Subject: electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Many thanks to everyone who responded to my question about
electropolishing. The details of my experiment are:
1. it is an Al-Mg-Si alloy.
2. it is a twin jet-polish by Tenupole
3. The flow rate I used was 6.
4. The black film possibly appeares at the beginning of the polishing
not after the foil prepared.
5. I noted that the current is more than 500 mA this time, not about
100 mA as before. This is a difference. Is it due to the problem of the
instruments.

Yan Hu
----------------------

yan.hu-at-brunel.ac.uk



From daemon Tue Sep 11 14:01:53 2001



From: stacey andringa :      andrina-at-email.uc.edu
Date: Tue, 11 Sep 2001 14:52:06 -0400
Subject: mouse ears

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone done serial sections of decalcified mouse ears? I have been
given some that were decalcified in EDTA for about 10 days,
post-osmicated, dehydrated, infiltrated and embedded in Spurr resin. A
grad student did the orienation for embedding, but along what plane I do
not know. Each orientation is a little different. Can anyone recommend
a consistent method of embedding?

Stacey.Andringa-at-uc.edu



From daemon Wed Sep 12 02:38:41 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Wed, 12 Sep 2001 09:19:42 +0200
Subject: Black Tuesday

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear friends and colleagues

This message is not relevant to our normal topics of discussion but
I hope that under the circumstances you, Nestor, and others will
forgive me for this once-off breach of the rules.

I am sure I speak on behalf of all subscribers to the MSA listserver
when I express heartfelt shock and indignation at yesterdays
tragedies in New York City, Washington DC and near Pittsburg.
Those of us from elsewhere in the world assure our colleagues in
the US that we share your grief, and offer our support at this
difficult time. We extend our sincere condolences to those who
have lost family members, friends and colleagues and we wish a
speedy recovery to those who have been injured.

God bless, vasbyt and sterkte*.

Rob

* loosely translated = "hang in there and be strong"


=======================================================
Robin H Cross
Director : Electron Microscopy Unit, and
Chairman : 15th International Congress on Electron Microscopy (ICEM-15)
Rhodes University, PO Box 94, Grahamstown, South Africa
tel: +27 46 603 8168/9, fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm
http://www.icem15.com
===========================================================================
Remember that ICEM-15 takes place in Durban, South Africa in September 2002
===========================================================================


From daemon Wed Sep 12 04:29:32 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 12 Sep 2001 10:34:19 +0100
Subject: Re: Ask-A-Microscopist:Germs and Science Projects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have watched this conversation with interest and must admit that I am
a very nervous about a student in a High School isolating pathogens on
blood agar at 37 deg C. This pre-supposes that there are trained
microbiologists, safe handling facilities and disposal methods for
unknown human pathogens.

If someone was to perform a risk assessment on this activity would they
even consider letting an unsupervised first year degree student isolate
unknown pathogens in the microbiology department?

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Jill Verlander Reed wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Katelyn,
} I will only add to Nelson Conti's remarks that you should be able to buy
} "blood-agar plates," which are Petri dishes already filled with a
} sterile, general bacterial culture medium, as well as sterile
} cotton-tipped swabs, from a medical supply store in your area. My
} daughter did a project along the same lines as yours and that's where we
} got the supplies. The dishes came in packs of 10, I think and they only
} cost a few dollars.
} We kept the inoculated dishes in a warm place. Around body temperature
} (37 degrees C, 98-99 degrees F) is ideal is you can find such a place.
} If you find bacterial colonies that have a clear ring around them,
} instead of the red of the rest of the blood agar, that is an indication
} that the bacteria in that colony are pathogenic (disease-producing).
} Good luck, and have fun.
}
} Jill Verlander Reed, D.V.M.
} Associate Scientist
} Director, College of Medicine Electron Microscopy Core Facility
} University of Florida
} P.O. Box 100215
} Gainesville, FL 32610
} verlaj-at-medicine.ufl.edu
} Phone: (352) 846-0820
} Fax: (352) 846-3299

===============================================================
ORIGINAL MESSAGE:

Email: sbcook-at-bcpl.net
Name: Katelyn Cook

Organization: St. Michael the Archangel

Education: 6-8th Grade Middle School

Location: Baltimore, MD

Question: I am planning to do a science fair project about germs and
the importance of hand washing. I want to collect germs from common
public places such as doorknobs, handrails, telephones, sink faucets,
etc. What is the best way to collect the germs? Will I need to grow
the germs in a petri dish after I collect them? Will I be able to
identify different types of germs using my microscope?

---------------------------------------------------------------------------


From daemon Wed Sep 12 07:48:03 2001



From: =?iso-8859-1?q?Valeria=20L=20Burgos?= :      valaburg-at-yahoo.com.ar
Date: Wed, 12 Sep 2001 09:38:51 -0300 (ART)
Subject: fixatives - a quick question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone! I wonder if this is the right place for my doubt... I
need if someone could tell me whether a major and important
difference exists between Paraformaldehyde 4% vs Formaldehyde4%
as a fixative for brain tissue.
Thank you
:)
Valeria Burgos

_________________________________________________________
¿Lo probaste?
Correo gratis y para toda la vida en http://correo.yahoo.com.ar


From daemon Wed Sep 12 07:54:55 2001



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 12 Sep 2001 07:49:17 -0500
Subject: mouse ears

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I embed and section mouse cochleas on a regular basis (anywhere between 1
and 4 microns in thickness). Is your tissue dissected down to the
spiral-shaped bone of the cochlea itself? Do you want sections along the
plane of the modiolus (the center columnar structure of the cochlea) so that
you get a cross-sectional view of three to five organ of Corti profiles?
The cochleas I embed still have some vestibular structures remaining.

If so, I embed the cochleas in flat silicone molds with the flat surface of
bone (which faces the brain) facing the bottom of the mold and the spiral
surface up. After the resin has been polymerized and the blocks removed
from the mold, I remount the block onto flat-ended cylinder blanks. (These
are made by closing the cap of a BEEM capsule, cutting off the pyramidal
tip, placing them with the closed cap down, filling them to the rim with
resin, polymerizing and removing the BEEM capsule.) The block is remounted
(I usually use SuperGlue) onto the blank with the cochlea side down (the
curved surface now faces down and the flat bony surface faces up). Excess
resin is removed by trimming the block as usual. When I'm cutting into the
block, I continually check the orientation. I know I'm close the proper
orientation if I start to get profiles of the maculae and/or cristae in the
vestibular structures and a base-to-apex view of the modiolus. Continue to
adjust until you get the desired orientation.

I hope this gets you started.

Jaclynn M. Lett, Research Technician III jlett-at-cid.wustl.edu

Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110

voice: 314-977-0257 fax: 314-977-0030

-----Original Message-----
} From: stacey andringa [mailto:andrina-at-email.uc.edu]
Sent: Tuesday, September 11, 2001 1:52 PM
To: Microscopy-at-sparc5.microscopy.com


Has anyone done serial sections of decalcified mouse ears? I have been
given some that were decalcified in EDTA for about 10 days,
post-osmicated, dehydrated, infiltrated and embedded in Spurr resin. A
grad student did the orienation for embedding, but along what plane I do
not know. Each orientation is a little different. Can anyone recommend
a consistent method of embedding?

Stacey.Andringa-at-uc.edu



From daemon Wed Sep 12 12:08:40 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Wed, 12 Sep 2001 11:59:08 -0500
Subject: LM - counting agglutinated blood cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all,

We have been working with hemagglutination tests lately (something
new for our EM lab), and are looking for a quick and easy way to
score fields captured using a standard LM with a digital camera.

We have tried some of the image analysis packages available, but have
not found them very easy to use. Also we have some questions about
the images themselves - how many blood cells have to be stuck
together before it's considered to be a clump? A clump is a 3
dimensional shape, so this has to be taken into account, doesn't it?

If anyone is doing this routinely and wouldn't mind giving us a few
hints (or references), we'd really appreciate it.

Thanks in advance for any help you can give.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca


From daemon Wed Sep 12 13:26:33 2001



From: Wang, Zhiyu :      Zhiyu_Wang-at-Maxtor.com
Date: Wed, 12 Sep 2001 13:18:26 -0500
Subject: RE: Spectral Imaging with ISIS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all:

The term of "Spectral Imaging" is not well defined yet. If it means the
distribution of certain number of elements on the surface of sample, that is
the X-ray mapping. If it means all of possibility on the element table
(means energy spectrum) on the entire surface of sample (mapping), I do not
think the Oxford ISIS can do it. The simple way is to use the "Area
Analysis" in stead of point analysis. That will give you all element
signature on the selected area.

In ISIS 300, there is an EBSD package attached (need to get key, again),
this package can do the similar thing. It collects the electron backscatter
diffraction pattern (EBSP) on a single point and compares it with a signed
crystallographic pattern (one needs to be known and signed). It does this
on an array point by point and displays the matrix as a map. The final
result shows the contrast of crystallography on the surface layer ( {50nm).
This can be called as the imaging of diffraction property of given crystal.
It is a very good tool for the study of grain size and boundary.

It is up to user's interest, what is your goal for "spectral imaging". ISIS
has another software package, call Particle Analyzer (Gun shot analyzer).
Again, user needs to give information about the most possible present
elements, and the software can do the rest to see if these elements are
shown up. Without these pre-defined information, software will have no
direction to go and have no goal to be achieved.

Regards,

Zhiyu Wang
Maxtor Corp.



-----Original Message-----
From: Zhiyuw [mailto:zhiyuw-at-home.com]
Sent: Tuesday, September 11, 2001 9:51 PM
To: zhiyu_wang-at-maxtor.com
Subject: Fw: Spectral Imaging with ISIS


----- Original Message -----
From: "Warren E Straszheim" {wesaia-at-iastate.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, August 23, 2001 10:32 AM
Subject: RE: Spectral Imaging with ISIS


}
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} The Microscopy ListServer -- Sponsor: The Microscopy
Society of America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
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}
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}
}
}
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Society of America
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}
-----------------------------------------------------------------------.
}
}
} I am pretty sure that Alwyn is not talking about x-ray
mapping. If that
was
} all he wanted, I suppose the key disk is all that would be
needed if he
} already has the other imaging applications and hardware
installed.
}
} Someone suggested using Auto to scan an image for multiple
spectra.
Indeed,
} the software and hardware should allow it. The spectra
might then be
} exported and processed by some other package to perform
the type of
} component analysis that has been described at MSA over the
last few years.
} My current ISIS x-ray application (version 3.32) has the
option of storing
} the x-ray data in single column MSA format. Previous
versions placed
} multiple channels on a single line.
}
} One hitch would be the limitation on the number of spectra
per job. I
think
} there is a limit of 1000 or so built into the database
used for managing
} the data. I suppose it could be changed to another number,
but we bumped
} into it some time back.
}
} That would mean you could do a 32x32 raster of points
saving a spectrum at
} each. A four second acquisition per point would mean a bit
more than an
} hour for acquisition. That would not be too bad, but the
spatial
resolution
} would probably be too low to be very useful. But the data
should readily
} fit onto hard drives with capacities of a few gigabytes.
}
} I would be interested in seeing how the data processing is
handled. If
} someone makes it work, I would be very interested in
hearing the details.
}
} Warren
}
} At 10:33 AM 8/23/2001 -0400, you wrote:
}
} -----------------------------------------------------------------------.
} } This is correct, the keydisk is all that is required for
} } the software to be enabled. However, there may be
hardware
} } required, too, at least a cable interface (RS232?) to the
} } microscope. I believe the ISIS Autobeam likes to select
} } its own scan rates. You may need to speak with Oxford
re:
} } compatibility with your particular microscope.
} }
} } Matt
} }
} } Matthew J. Lynn
} } Center for Advanced Microscopy
} } University of Miami
} } (305)284-4736
} } mlynn-at-miami.edu
} }
} }
} } On Wednesday, August 22, 2001 9:23 PM, Fred Pearson
} } [SMTP:eoptics-at-mcmail.cis.mcmaster.ca] wrote:
} } }
} } } Alwyn:
} } }
} } } By spectral images, do you mean xray mapping? A "key"
disk for the
ISIS
} } } software program is required to activate the xray
mapping facility.
Of,
} } } course this will cost a bit of money to purchase from
Oxford. When
the
} } } ISIS program is loaded initially, all the software
required to use the
} } } program is there, but activating the unpaid for parts
of the program
is
} } } the thing.
} } }
} } } Fred
} } }
} } } On Wed, 22 Aug 2001, Alwyn Eades wrote:
} } } } We have an Oxford ISIS EDS system. As it stands it
is not set up to
} } } } acquire spectral images. We would like to be able
to do spectral
image
} } } } acquisition on this machine. Is there anyone out
there who knows
how
} } } } the system may be modified (macros, interfaces,
whatever) to do it?
We
} } } } do have Digital Micrograph (on a Mac whereas ISIS is
on a PC), if
that
} } } } helps.
} } } } ..........
} } } } Alwyn Eades
} } } } Department of Materials Science and Engineering
} } } } Lehigh University
} } } } 5 East Packer Avenue
} } } } Bethlehem
} } } } Pennsylvania 18015-3195
} } } } Phone 610 758 4231
} } } } Fax 610 758 4244
} } } } jae5-at-lehigh.edu
}
}
}


From daemon Wed Sep 12 13:45:41 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Wed, 12 Sep 2001 14:38:22 -0400
Subject: Postdoctoral Position Available immediately

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all,

I am posting this for a colleague.

Paula

___________________________________________________________

Postdoctoral Position Available

Wageningen University
Dept Agrotechnology and Food Sciences

Postdoctoral Research Position in Cell Wall Microscopy

A vacancy for a Postdoctoral Position is available in the area of microscopy and functionality of cell walls as part of the research program of Dr. Henk Schols, Laboratory of Food Chemistry. The successful candidate will be studying serum separation in tomato products and the influence of processing at the cell wall level. Ideally immunolocalization techniques will be used to localise specific polysaccharides in these samples.

Candidates must originate from outside The Netherlands and hold a recent Ph.D. in a related field. The appointment is for 9 months and is available immediately.

For more information, interested candidates should contact:


Dr. Henk Schols
Wageningen University
Dept Agrotechnology and Food Sciences
Laboratory of Food Chemistry
Bomenweg 2
6703 HD Wageningen
The Netherlands

tel 31 317 482239
fax 31 317 484893

E-mail: Henk.Schols-at-chem.fdsci.wag-ur.nl
http://www.ftns.wau.nl/lmt/lmc/


¯---------------------------------------------------------------------------------------------------------------------

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Wed Sep 12 14:43:57 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 12 Sep 2001 15:35:10 -0400
Subject: Re: Black Tuesday

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thank You Randy.

Here is Washington, DC things are starting to get back to normal. Though what is normal has now changed. Our city was shut down for one day by cowardly terrorist acts. We are back at work and are carrying on. We will not let this break our spirit. The "Sleeping Giant" has been aroused and it is much larger than the perpetrators of this act realized as the Giant includes the global community.

Courage,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

} } } "R. Cross" {r.cross-at-ru.ac.za} 09/12/01 03:19AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear friends and colleagues

This message is not relevant to our normal topics of discussion but
I hope that under the circumstances you, Nestor, and others will
forgive me for this once-off breach of the rules.

I am sure I speak on behalf of all subscribers to the MSA listserver
when I express heartfelt shock and indignation at yesterdays
tragedies in New York City, Washington DC and near Pittsburg.
Those of us from elsewhere in the world assure our colleagues in
the US that we share your grief, and offer our support at this
difficult time. We extend our sincere condolences to those who
have lost family members, friends and colleagues and we wish a
speedy recovery to those who have been injured.

God bless, vasbyt and sterkte*.

Rob

* loosely translated = "hang in there and be strong"


=======================================================
Robin H Cross
Director : Electron Microscopy Unit, and
Chairman : 15th International Congress on Electron Microscopy (ICEM-15)
Rhodes University, PO Box 94, Grahamstown, South Africa
tel: +27 46 603 8168/9, fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm
http://www.icem15.com
===========================================================================
Remember that ICEM-15 takes place in Durban, South Africa in September 2002
===========================================================================




From daemon Wed Sep 12 14:57:09 2001



From: Dmrelion-at-aol.com
Date: Wed, 12 Sep 2001 15:52:09 EDT
Subject: Wild M5A microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Anyone know if there was ever a trinocular head available for a Wild M5A
binocular microscope and, if so, where to get a used one?

Thanks,

Don

Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Wed Sep 12 15:07:56 2001



From: Thimothy Schneider :      timothy.schneider-at-mail.tju.edu
Date: Wed, 12 Sep 2001 16:05:57 -0400
Subject: BLACK TUESDAY

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers:

I work on the University side of a large Medical center and yesterday after
the attack in New York and Washington we received a broadcast e mail from
the administration asking for blood. A couple of hours later I got to the
blood bank and found a huge mob of medical workers in line to donate blood.
Today the line was more then three hours long. I am going to wait a couple
of days before donating blood. I think its realalistic that the demand for
blood will be very high for at least a few weeks to come. I urge all
healthy adults to join me in donating blood as its the only real thing that
private citizens can do to help in this unprecedented catastrophe.
Sincerely, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 JAH
1020 Locust Street
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cell
Timothy.Schneider-at-Mail.TJU.edu



From daemon Wed Sep 12 15:45:48 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Wed, 12 Sep 2001 15:39:05 -0500
Subject: Re: mouse ears

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Stacey,

There are a couple of ways to reduce the orientation variation. One way
is to use flat-bottomed Beem capsules for embedding molds (I haven't got
the order info on these with me, but I can get it to you later, or you
may get a reply from someone who has the info handy). These molds have
a small enough surface area at the bottom that you can place the bulla
or
inner ear in them, say medial side down and they don't rock or float
out of position. (Fill the mold before manipulating the specimen.)

Another way I have for embedding the cochlea is to split it in half,
down the modiolus just prior to embedding and place the cut surface
face down in the molds. I use half of a double edged razor blade to
split it under a dissecting scope and I orient the cut from the apex
to the base with the round window membrane perpendicular to the top
surface. If you process the cochlea to this point before you split it,
it is much firmer and tougher to work with and you won't lose as much
tissue, but you do lose some. Hope this helps.

Karen Pawlowski, Ph.D.

stacey andringa wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Has anyone done serial sections of decalcified mouse ears? I have been
} given some that were decalcified in EDTA for about 10 days,
} post-osmicated, dehydrated, infiltrated and embedded in Spurr resin. A
} grad student did the orienation for embedding, but along what plane I do
} not know. Each orientation is a little different. Can anyone recommend
} a consistent method of embedding?
}
} Stacey.Andringa-at-uc.edu


From daemon Wed Sep 12 16:00:50 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Wed, 12 Sep 2001 15:56:02 -0500
Subject: Re: Ask-A-Microscopist:Germs and Science Projects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Malcolm,

I find your comment interesting as I have volunteered to judge several
Regional and National Science Fairs for high school aged students and
have seen this particular experiment carried out by a number of the
students. The students that I choose to judge are actually the younger
group, who are "middle school" aged and I doubt the majority of them
are getting much outside help, other than their science teachers or
they would have picked a topic that was more unique (or geared to what
the expert was doing).

I'm sure the petri dishes never leave the science lab. and the students
were gloves while handling the cultures, but that is probably the
extent of it....

Karen Pawlowski, Ph.D.


Malcolm Haswell wrote:
}
} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have watched this conversation with interest and must admit that I am
} a very nervous about a student in a High School isolating pathogens on
} blood agar at 37 deg C. This pre-supposes that there are trained
} microbiologists, safe handling facilities and disposal methods for
} unknown human pathogens.
}
} If someone was to perform a risk assessment on this activity would they
} even consider letting an unsupervised first year degree student isolate
} unknown pathogens in the microbiology department?
}
} Malcolm Haswell
} e.m. unit
} School of Sciences
} University of Sunderland
} UK
}
} Jill Verlander Reed wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Dear Katelyn,
} } I will only add to Nelson Conti's remarks that you should be able to buy
} } "blood-agar plates," which are Petri dishes already filled with a
} } sterile, general bacterial culture medium, as well as sterile
} } cotton-tipped swabs, from a medical supply store in your area. My
} } daughter did a project along the same lines as yours and that's where we
} } got the supplies. The dishes came in packs of 10, I think and they only
} } cost a few dollars.
} } We kept the inoculated dishes in a warm place. Around body temperature
} } (37 degrees C, 98-99 degrees F) is ideal is you can find such a place.
} } If you find bacterial colonies that have a clear ring around them,
} } instead of the red of the rest of the blood agar, that is an indication
} } that the bacteria in that colony are pathogenic (disease-producing).
} } Good luck, and have fun.
} }
} } Jill Verlander Reed, D.V.M.
} } Associate Scientist
} } Director, College of Medicine Electron Microscopy Core Facility
} } University of Florida
} } P.O. Box 100215
} } Gainesville, FL 32610
} } verlaj-at-medicine.ufl.edu
} } Phone: (352) 846-0820
} } Fax: (352) 846-3299
}
} ===============================================================
} ORIGINAL MESSAGE:
}
} Email: sbcook-at-bcpl.net
} Name: Katelyn Cook
}
} Organization: St. Michael the Archangel
}
} Education: 6-8th Grade Middle School
}
} Location: Baltimore, MD
}
} Question: I am planning to do a science fair project about germs and
} the importance of hand washing. I want to collect germs from common
} public places such as doorknobs, handrails, telephones, sink faucets,
} etc. What is the best way to collect the germs? Will I need to grow
} the germs in a petri dish after I collect them? Will I be able to
} identify different types of germs using my microscope?
}
} ---------------------------------------------------------------------------


From daemon Wed Sep 12 20:22:17 2001



From: Gerry Nash :      gerry.nash-at-antdiv.gov.au
Date: Thu, 13 Sep 2001 11:13:51 +1000
Subject: For the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


To all our Amercian fellow microscopists and friends,
The Australian Society for Electron Microscopy Inc sends its deepest
heartfelt condolences to you all and your loved ones at this time of
such tragic and unbelievable loss.
Our warmest thoughts and prayers are with you all.
Gerry Nash
President ASEM Inc
--
Geraldine (Gerry) Nash
Electron Microscopist

EM Unit
Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050
Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323 351

Visit our EM Unit web site:
http://www.aad.gov.au/science/ResearchResources/em_unit/default.asp
and
Australian Antarctic Division web site: http://www.aad.gov.au/


From daemon Wed Sep 12 20:25:22 2001



From: Gerry Nash :      gerry.nash-at-antdiv.gov.au
Date: Thu, 13 Sep 2001 11:19:25 +1000
Subject: For the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


To all our Amercian fellow microscopists and friends,
The Australian Society for Electron Microscopy Inc sends its deepest
heartfelt condolences to you all and your loved ones at this time of
such tragic and unbelievable loss.
Our warmest thoughts and prayers are with you all.
Gerry Nash
President ASEM Inc
--
Geraldine (Gerry) Nash
Electron Microscopist

EM Unit
Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050
Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323 351

Visit our EM Unit web site:
http://www.aad.gov.au/science/ResearchResources/em_unit/default.asp
and
Australian Antarctic Division web site: http://www.aad.gov.au/


From daemon Thu Sep 13 07:22:29 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Thu, 13 Sep 2001 08:07:53 -0400 (EDT)
Subject: Re: For the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thank you, Gerry.

Best regards,

Angela

Angela V. Klaus

Director, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977


On Thu, 13 Sep 2001, Gerry Nash wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To all our Amercian fellow microscopists and friends,
} The Australian Society for Electron Microscopy Inc sends its deepest
} heartfelt condolences to you all and your loved ones at this time of
} such tragic and unbelievable loss.
} Our warmest thoughts and prayers are with you all.
} Gerry Nash
} President ASEM Inc
} --
} Geraldine (Gerry) Nash
} Electron Microscopist
}
} EM Unit
} Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050
} Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323 351
}
} Visit our EM Unit web site:
} http://www.aad.gov.au/science/ResearchResources/em_unit/default.asp
} and
} Australian Antarctic Division web site: http://www.aad.gov.au/
}
}



From daemon Thu Sep 13 07:47:10 2001



From: Alexander Black :      alexander.black-at-nuigalway.ie
Date: Thu, 13 Sep 2001 13:31:51 +0100
Subject: Heartfelt sympathy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


To all our American colleagues:
On behalf of the Microscopical Society of Ireland, I would like to
extend
deepest sympathy and condolences to those who have been affected
directly or
indirectly by the atrocious terrorist attacks on the American Nation.

Sincerely,
Alexander Black,
President,
Microscopical Society of Ireland



Department of Anatomy
National University of Ireland, Galway.





From daemon Thu Sep 13 09:26:10 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 13 Sep 2001 10:16:02 -0400
Subject: RE: fixatives - a quick question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Morning Valeria,

There is a great difference. Paraformaldehyde is a POLYMER of
formaldehyde(HCHO). Paraformaldehyde will yield HCHO if you heat the dry
powder to around 90 degrees C (NOT RECOMMENDED!!!). I am sending a document
under separate cover that will explain, but briefly, there are three words
that are important here. Formalin (commercial, 40% HCHO solution in HOH,
that contains from 5-15% methanol to prevent the HCHO from polymerizing),
Paraformaldehyde (the polymer, a white powder), and formaldehyde solutions
(generated from paraformaldehyde suspensions in water or buffer, which I
designate as "pHCHO"). The attachment to the following email will explain
in greater detail.
Good question!!!

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Thu Sep 13 11:40:28 2001



From: tea meulia :      meulia.1-at-osu.edu
Date: Thu, 13 Sep 2001 12:39:35 -0500
Subject: anti-dsDNA antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for antibodies against double-stranded DNA, and eventually
antibodies against histones. The two companies that I contacted, Biogenex
and Pierce, discontinued its productions. Does anybody know for a supplier?

Tea


***************************************
Tea Meulia
Research Scientist and Director
Molecular and Cellular Imaging Center
Ohio State University/OARDC
1680 Madison Ave.
Wooster OH 44691

tel.: 330-263-3836 or -3828
fax: 330-202-3563

http://www.oardc.ohio-state.edu/mcic

*****************************************




From daemon Thu Sep 13 17:39:01 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Thu, 13 Sep 2001 15:24:01 -0700
Subject: Announcement correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
We have had a last minute change in the program for our program for
Microscopy and Digital Imaging on Sept. 27, 2001

1:15 "Imaging Tissue Morphogenesis in Zebrafish Embryos."
Mark Cooper, University of Washington

Regards,
Glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************

C:} The box said "Requires Windows95 or better". So I bought a
Macintosh.
**************************************************************************





From daemon Thu Sep 13 21:32:38 2001



From: Edward_Principe-at-amat.com
Date: Thu, 13 Sep 2001 19:20:38 -0700
Subject: Re: For the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



In regard to what is "appropriate", I share my own view.

I agree that science and this list server under these circumstances should
be divorced from any shade of political statement.

The science community (emphasis on the community) is not divorced from
emotion, thank goodness.

I believe we graciously accept the simple gestures of sympathy and not
imply insult or rejection.

Justification: It is a historical, if not still surreal and forever
infamous, time for world civilization.

With best regards and appreciation,
Ed


Angela Klaus {avklaus-at-amnh.org} on 09/13/2001 05:07:53 AM


To: Gerry Nash {gerry.nash-at-antdiv.gov.au}
cc: microscopy-at-sparc5.microscopy.com


Thank you, Gerry.

Best regards,

Angela

Angela V. Klaus

Director, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977


On Thu, 13 Sep 2001, Gerry Nash wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To all our Amercian fellow microscopists and friends,
} The Australian Society for Electron Microscopy Inc sends its deepest
} heartfelt condolences to you all and your loved ones at this time of
} such tragic and unbelievable loss.
} Our warmest thoughts and prayers are with you all.
} Gerry Nash
} President ASEM Inc
} --
} Geraldine (Gerry) Nash
} Electron Microscopist
}
} EM Unit
} Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050
} Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323
351
}
} Visit our EM Unit web site:
} http://www.aad.gov.au/science/ResearchResources/em_unit/default.asp
} and
} Australian Antarctic Division web site: http://www.aad.gov.au/
}
}






From daemon Fri Sep 14 09:30:58 2001



From: Glenn Poirier :      glennp-at-eps.mcgill.ca
Date: 14 Sep 2001 10:11:51 -0400
Subject: Wehnalt cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
About a year ago there was a discussion about using ammonia to clean
Wehnelts. I dutifully saved all the emails because the technique looked
interesting. Of course, when I went looking for these emails I couldn't
find them. I looked on the list archives but was not able to come up
with anything. Could someone who uses this technique contact me off
list?

Thanks
--
Glenn

===============================================================================
Glenn Poirier 3450 University St, rm. 238
MicroAnalytical Laboratory Montreal, Qc
Earth and Planetary Sciences tel (514) 398 6774
McGill University fax (514) 398 4680
email: glennp-at-eps.mcgill.ca http://castaing.eps.mcgill.ca

++ Millenium hand and shrimp ++
===============================================================================



From daemon Fri Sep 14 09:48:18 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 14 Sep 2001 10:38:30 -0400
Subject: Re: For the USA

Contents Retrieved from Microscopy Listserver Archives
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for dist-Microscopy; Fri, 14 Sep 2001 09:42:47 -0500 (CDT)
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Importance: Normal


Thank you, all, for your support. I would like to share an article I
received
with everyone, especially my fellow Americans. I hope nobody is offended
by it, for I do not intend, or wish, for that to happen.

Darrell

} I know you are all receiving a lot of emails like this today...but read
} on:
}
} Subject: This, from a Canadian newspaper, is worth sharing.
}
} America: The Good Neighbor.
}
} Widespread but only partial news coverage was given recently to
} remarkable editorial broadcast from Toronto by Gordon Sinclair,
} a Canadian television commentator.
} What follows is the full text of his trenchant remarks as
} printed in the Congressional Record:
}
} "This Canadian thinks it is time to speak up for the Americans as
} the most generous and possibly the least appreciated people
} on all the earth.
} Germany, Japan and, to a lesser extent, Britain and Italy were
} lifted out of the debris of war by the Americans who poured in
} billions of dollars and forgave other billions in debts.
} None of these countries is today paying even the interest
} on its remaining debts to the United States.
}
} When France was in danger of collapsing in 1956, it was the
} Americans who propped it up, and their reward was to be insulted
} and swindled on the streets of Paris. I was there. I saw it.
}
} When earthquakes hit distant cities, it is the United States
} that hurries in to help. This spring, 59 American communities
} were flattened by tornadoes. Nobody helped.
}
} The Marshall Plan and the Truman Policy pumped billions of dollars
} into discouraged countries. Now newspapers in those countries are
} writing about the decadent, warmongering Americans.
}
} I'd like to see just one of those countries that is gloating over
} the erosion of the United States dollar build its own airplane.
} Does any other country in the world have a plane to equal
} the Boeing Jumbo Jet, the Lockheed Tri-Star, or the Douglas DC10?
} If so, why don't they fly them?
} Why do all the International lines except Russia fly American
} Planes?
}
} Why does no other land on earth even consider putting a man or
} woman on the moon?
} You talk about Japanese technology, and you get radios.
} You talk about German technology, and you get automobiles.
}
} You talk about American technocracy, and you find men on the
} moon-not once, but several times-and safely home again.
} You talk about scandals, and the Americans put theirs right
} in the store window for everybody to look at.
}
} Even their draft-dodgers are not pursued and hounded. They are
} here on our streets, and most of them, unless they are breaking
} Canadian laws, are getting American dollars from ma and pa at home
} to spend here.
}
} When the railways of France, Germany and India were breaking down
} through age, it was the Americans who rebuilt them.
} When the Pennsylvania Railroad and the New York Central went broke,
} nobody loaned them an old caboose.
} Both are still broke.
}
} I can name you 5000 times when the Americans raced to the help of
} other people in trouble. Can you name me even one time when
} someone else raced to the Americans in trouble?
} I don't think there was outside help even during the San Francisco
} earthquake.
}
} Our neighbors have faced it alone, and I'm one Canadian who is
} damned tired of hearing them get kicked around.
} They will come out of this thing with their flag high.
} And when they do, they are entitled to thumb their nose at
} the lands that are gloating over their present troubles. I hope
} Canada is not one of those."
}
} Stand proud, America!
}
} I would hope that each of you would send this to as many people
} as you can and emphasize that they should send it to as many
} of their friends until this letter is sent to every person on the web.



From daemon Fri Sep 14 10:17:02 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 14 Sep 2001 11:16:01 -0400
Subject: RE: Cleaning WWehnelt cylinders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Various methods for cleaning Wehnelt cylinders, and other internal
parts of vacuum systems, are discussed in some detail in Section
2.10.4c (see bottom of page 72) of the book Vacuum Methods in
Electron Microscopy (for info about this book see:
http://www.2spi.com/catalog/books/book48.html and
http://pup.princeton.edu/titles/6484.html).

Disclaimer: I make about $1.50 from each copy of this book sold.
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Fri Sep 14 10:44:19 2001



From: steven wintonick :      crimsem-at-hotmail.com
Date: Fri, 14 Sep 2001 11:34:24 -0400
Subject: Thanks from NYC and USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


To all those people who have expressed their concerns,

I would like to thank all of those people who have sent their heartfelt
messages and words of encouragement. Especially now in our time of need, it
is uplifting to know all of our fellow microscopists from other nations are
expressing their concern and support. During this time, when it is difficult
to sort out our emotions, all of your words have helped to restore some of
my faith in humanity.

Thanks you.
Steve

_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp



From daemon Fri Sep 14 12:10:57 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 14 Sep 2001 12:59:31 -0400
Subject: Please accept my apology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I must humbly apologize for the "article" I sent. A coworker
has informed me that it is a hoax. My first instinct was to not
send it. I made the mistake of trusting the source, and allowing
the video images of crowds of people cheering the deaths of
tens of thousands of Americans in the matter of a few hours,
cloud my better judgment.

I was, and am, sincere in my thanks for the support expressed
on the list, and from all other sources.

Darrell

(These are my opinions. You can blame no one else for them.)



From daemon Fri Sep 14 12:19:17 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com (by way of Nestor J.
Date: Fri, 14 Sep 2001 11:53:14 -0500
Subject: Have VP-SEM with tensile stage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

Usually we do our own SEM work, but a special job requirement may arise
which I cannot accommodate using our equipment. It may be desirable to
tensile fracture a thin ceramic sheet (predominately zirconia) and examine
the fracture surface without breaking vacuum. EDS would likely be required
as well. Magnification requirements *should* range from about 20x to 5000x.
It would not surprise me, however, if 30-50 Kx might provide useful
information.

If you have the equipment and take "outside" work, please contact me by
direct email or phone.

Direct line to my office and lab: 434.522.6111 ...If A/C 434 fails, try
the old one: 804

Thanks,

Woody White
McDermott Technology, Inc.
Lynchburg Research Center
Lynchburg, VA


McDermott site: http://www.mtiresearch.com/
Personal site: http://woody.white.home.att.net


From daemon Fri Sep 14 15:04:51 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 14 Sep 2001 12:54:51 -0700
Subject: Re: Wehnalt cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Glenn

I am not sure is it original recipe or it's my modification, but anyway,
the following works for me. I am sonicate Wehnelt cup in 1:1 diluted
ammonia solution (stock ammonia solution I believe is 30%) for 10-15 min,
rinse with deionized water and dry at +60 in the oven.

Good luck. Sergey



At 10:11 AM 9/14/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Sep 14 17:40:54 2001



From: Kristof Kovacs :      kris-at-almos.vein.hu
Date: Fri, 14 Sep 2001 09:09:09 +0200
Subject: Sorrow and sympathy

Contents Retrieved from Microscopy Listserver Archives
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Wehnelt caps are usually made from stainless steel (304).

For JEOL wehnelts, I have had customers use "ammonium hydroxide hydrogen
peroxide 30% by volume". I am not a chemist so I can't give you the
particulars but this works great as it attacks everything except the
stainless steel. I usually let the cap soak in this solution overnight.


Regards,

Earl weltmer
----- Original Message -----
} From: "Glenn Poirier" {glennp-at-eps.mcgill.ca}
To: "Microscopy Listserver" {microscopy-at-sparc5.microscopy.com}
Sent: Friday, September 14, 2001 7:11 AM


Dear American Fellow Microscopists,
After the first moments of shock and grief let us express our deepest
sorrow and sympathy to the American microscopists in particular and to the
American people in general on behalf of all Hungarian microscopists. We
have so many friends and colleagues there...
To overcome these hard days and weeks let us wish you all strength both
physically and in your soul, work and fight together for a better world!
Kristof Kovacs

-----------------------------------------------------------
Dr. Kristof Kovacs
President, Hungarian Society for Microscopy
University of Veszprem
Veszprem, P.O.Box 158
H-8201 HUNGARY
Phone: +36-(88)-421-684, +36-(30)-930-3931
Fax: +36-(88)-423-091



From daemon Sun Sep 16 09:49:38 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Sun, 16 Sep 2001 10:29:20 -0400
Subject: Food Structure and Functionality Symposium 2002 - First

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Food Structure & Functionality Symposium 2002
May 5 - 8, 2002, Palais des Congrès de Montréal o Montréal, Québec, Canada.

An international symposium leading Food Structure & Functionality studies through the 21st century
"webaddress at the AOCS site (http://www.aocs.org/member/division/fsff/index.htm)"

Being held in conjunction with the 93nd AOCS Annual Meeting & Expo (www.aocs.org)

The symposium has two themes:
* new and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure-function relationships in foods;
* food system studies covering any part of the processing chain - from the raw material to the final product, and including trouble shooting.

Tentative Program Schedule
Sunday, May 5th
Short Course - Understanding structure-function relationships in food systems through specific localisation methods and microscopy. Contact: Marcel Paques (Paques-at-nizo.nl)

Monday, May 6th
Morning

Opening of symposium
Plenary Speaker
Dairy Applications Session.
Chairs: Mark Auty (mauty-at-moorepark.teagasc.ie) and Harjinder Singh (H.Singh-at-massey.ac.nz)
Lunch break
Afternoon
Colloidal and Interfacial Sciences Session.
Chairs: Marcel Paques (Paques-at-nizo.nl) and David Pechak (Dpechak-at-kraft.com)

Dedicated Poster Session
Division Board Meeting

Tuesday, May 7th
Morning
Agricultural Applications of Microscopy and Imaging Session./ joint with Feed Microscopy Division. Topic/Tentative title (will probably be revised): New Microscopic Techniques for Identifying Food/Feed Constituents and Contaminants
contacts: Mark Auty (mauty-at-moorepark.teagasc.ie) and Kim Koch

Lunch break: Division Luncheon and round table (expert) discussion. Topic TBA
Afternoon
Microbiology and Food Session.
Chairs Judy Arnold (jarnold-at-saa.ars.usda.gov) and Ida Yates (iyates-at-ars.usda.gov)

Food Structure and Functionality Forum - Division Members Meeting

Wednesday, May 8th
Morning
Ingredients and Food Processing Session.
Chairs: Diana Kittleson (dkittleson-at-pillsbury.com) and Bernhard Tauscher (bernhard.tauscher-at-bfe.uni-karlsruhe.de)

Lunch break
Afternoon
New Methods and Techniques for Food Structure and Functionality Analysis Session.
Chairs: Kathy Groves Kgroves-at-LFRA.co.uk and Maud Langton maud.langton-at-sik.se

Closure of Symposium.

*--------------------------------------------------------------------------------------------------------------------
For further information, please go to the websites indicated, contact session chairs listed above, or myself.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Sun Sep 16 19:03:32 2001



From: Cathy Gillespie :      cathy.gillespie-at-anu.edu.au
Date: Mon, 17 Sep 2001 09:55:36 +1000
Subject: TEM of wool

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Can anyone recommend a method for preparing wool from sheep for TEM? Is it
easy to section?

Thanks

Cathy Gillespie



From daemon Sun Sep 16 19:06:08 2001



From: Peter Jordan :      emsi-at-pe.net
Date: Sun, 16 Sep 2001 16:57:12 -0700
Subject: Re: LETTER TO A TERRORIST...

Contents Retrieved from Microscopy Listserver Archives
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Well, you hit the World Trade Center, but you missed America. You hit the
Pentagon, but you missed America. You used helpless American bodies, to take
out other American bodies, but like a poor marksman, you STILL
missed America.

Why? Because of something you guys will never understand. America isn't
about a building or two, not about financial centers, not about military
centers, America isn't about a place, America isn't even about a bunch of
bodies. America is about an IDEA. An idea, that you can go someplace where
you can earn as much as you can figure out how to, live for the most part
like you envisioned living, and pursue Happiness. (No guarantees that you'll
reach it, but you can sure try!)

Go ahead and whine your terrorist whine, and chant your terrorist litany:
"If you cannot see my point, then feel my pain." This concept is alien to
Americans. We live in a country where we don't have to see your point. But
you're free to have one. We don't have to listen to your speech. But you're
free to say one. Don't know where you got the strange idea that everyone has
to agree with you. We don't agree with each other in this country, almost as
a matter of pride.

We're a collection of guys that don't agree, called States. We united our
individual states to protect ourselves from tyranny in the world. Another
idea, we made up on the spot. You CAN make it up as you go, when it's your
country. If you're free enough. Yeah, we're fat, sloppy, easygoing goofs
most of the time. That's an unfortunate image to project to the world, but
it comes of feeling free and easy about the world you live in. It's
unfortunate too, because people start to forget that when you attack
Americans, they tend to fight like a
cornered badger. The first we knew of the War of 1812 was when England
burned Washington, DC, to the ground. Didn't turn out like England thought
it was going to, and it's not going to turn out like you think, either.

Sorry, but you're not the first bully on our shores, just the most recent.
No Marquis of Queensbury rules for Americans, either. We were the FIRST
and, so far, only country in the world to use nuclear weapons in anger.
Horrific
idea, nowadays? News for you bucko, it was back then, too, but we used it
anyway. Only had two of them in the whole world and we used 'em both.

Grandpa Jones worked on the Manhattan Project. Told me once that right up
until they threw the switch, the physicists were still arguing over whether
the Uranium alone would fission, or whether it would start a fissioning
chain reaction that would eat everything. But they threw the switch anyway,
because we had a War to win. Does that tell you something about American
Resolve?
}
So who just declared War on us? It would be nice to point to some real
estate, like the good old days. Unfortunately, we're probably at war with
random camps, in far-flung places. Who think they're safe. Just like the
Barbary Pirates did. Better start sleeping with one eye open. There's a
spirit that tends to take over people who come to this country, looking for
opportunity, looking for liberty, looking for freedom. Even if they misuse
it. The Marielistas that Castro emptied out of his prisons, were overjoyed
to find out how much freedom there was. First thing they did when they hit
our shores, was run out and buy guns. The ones that didn't end up dead,
ended up in prisons. It was a big PITA then (especially in south Florida),
but you're only the newest PITA, not the first.

You guys seem to be incapable of understanding that we don't live in
America, America lives in US! American Spirit is what it's called. And
killing a few thousand of us, or a few million of us, won't change it. Most
of the time, it's a pretty happy-go-lucky kind of Spirit. Until we're
crossed in a cowardly manner, then it becomes an entirely different kind of
Spirit.

Wait until you see what we do with that Spirit, this time. Sleep tight, if
you can. We're coming. And when we arrive, well, let's just say it won't
be pretty.




From daemon Sun Sep 16 20:13:03 2001



From: Gao Yihua :      GAO.Yihua-at-nims.go.jp
Date: Tue, 17 Sep 2013 10:06:04 +0900
Subject: thermochemical data of Ge3N4

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleages,

Anyone knows which book or paper contains the thermochemical data of
Ge3N4 material.

Thank you very much in advance.


Yours sincerely

Gao Yihua


From daemon Mon Sep 17 05:46:46 2001



From: Martin Saunders :      martin-at-cmm.uwa.edu.au
Date: Mon, 17 Sep 2001 18:40:16 +0800
Subject: TEM sample prep: Ti extraction replica

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I have been given a piece of titanium that contains small
precipitates of unknown composition and asked to prepare a TEM sample
so that the precipitates can be analysed. As it is only the
precipitates that are of interest I thought that the best approach
would be to make an extraction replica. However, to do that need to
find a way to etch away the titanium matrix while leaving behind the
unknown precipitates.

I would be grateful for any recommendations for a suitable etchant
that will preferentially remove the titanium matrix so that the
extraction replica can be made.

Regards,

Martin Saunders.
--

*****************************************

Dr. Martin Saunders,
Lecturer,
Centre for Microscopy and Microanalysis,
University of Western Australia,
Crawley,
Western Australia 6009,
Australia.

Phone: +61 8 9380 8092
Fax: +61 8 9380 1087
E-mail: martin-at-cmm.uwa.edu.au

*****************************************


From daemon Mon Sep 17 07:43:58 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Mon, 17 Sep 2001 08:35:23 -0400
Subject: Responsible reactions

Contents Retrieved from Microscopy Listserver Archives
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Colleagues and Friends

It has been very positive to see, on the microscopy listserver, messages
from microscopy communities in other nations addressed to those of us
who live and work in the United States offering sympathy for the tragic
events of last Tuesday. These messages have, without exception, been
measured, responsible and generous. We are grateful for them.

Speaking for myself, I want to say how offended I was to read, on the
microscopy listserver, "Letter to a Terrorist" from Peter Jordan.
There are no terrorists on this list server. This is not an appropriate
place for this kind of polemic. I hope that those who take exception
to Mr. Jordan's remarks will refrain from responding to the substance of
what he says. The last thing we want is for the listserver to be taken
over by a political debate on the issues raised by the attacks on New
York and Washington.

Alwyn
--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Mon Sep 17 07:44:02 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Mon, 17 Sep 2001 22:37:30 +1000
Subject: to solute fat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues

I like to dissolve fat in insects bodies what could be the best chemical
. benzine, carbon tetrachloride, or???

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).




From daemon Mon Sep 17 08:00:58 2001



From: Haeseong Lee :      haeseong-at-hanyang.ac.kr
Date: Mon, 17 Sep 2001 07:55:50 -0500
Subject: SEM imaging on CNT

Contents Retrieved from Microscopy Listserver Archives
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??
Hello.

Could anybody recommend the good method of sample preparation for SEM
imaging on carbon nano tubes?
The sample is a powder type including single wall carbon nanotubes (SWCNTs).
Thank you very much in advance.

Haeseong Lee

{mailto:haeseong-at-hanyang.ac.kr} haeseong-at-hanyang.ac.kr


From daemon Mon Sep 17 09:10:52 2001



From: efosten-at-mmm.com
Date: Mon, 17 Sep 2001 09:02:44 -0500
Subject: Re: For the USA

Contents Retrieved from Microscopy Listserver Archives
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""} Subject: This, from a Canadian newspaper, is worth sharing.
}
} America: The Good Neighbor.
}
} Widespread but only partial news coverage was given recently to
} remarkable editorial broadcast from Toronto by Gordon Sinclair,
} a Canadian television commentator.
} What follows is the full text of his trenchant remarks as
} printed in the Congressional Record:""

Gordon Sinclair's piece was first broadcast in 1973 - see:

http://www.tysknews.com/Depts/Our_Culture/americans.htm


But it still applies.

Ev Osten

efosten-at-mmm.com
651-736-0104
fax: 651-733-0648

3M Company
Corporate Analytical Technology Center
3M Center, 201-BE-16
St. Paul, MN 55144-1000
U.S.A.



From daemon Mon Sep 17 09:54:00 2001



From: Ubirajara Pereira Rodrigues Filho :      uprf-at-iqsc.sc.usp.br
Date: Mon, 17 Sep 2001 11:42:57 -0300
Subject: thanks to answers on SEM of tempers in potteries ( specially shells )

Contents Retrieved from Microscopy Listserver Archives
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Dear collegues;

I'd like to thanks to all who has collaborate for the answers on tempers
studies on potteries by SEM. I hope in brief get in touch with some of them.

I was trying to don't say a word on the terrorism subject, that because
sometimes for me when something so horrible happens with me I prefer stay
quiet on my side in order to recover my balance to overcome such pain.
However, I would like to expreess my deep feelings to American people and
gouvernement. Since the attacks I could not stop to think how terrible the
human been can be, even if sometimes the streets can show us terrible facts.
I've watched several chanels, brazilian and foreigners, and still can
understand what hapens and why that people has died. I would like to remark
my sympathy to your gouvernement which, in my oppinion, is doing their job
in this difficult situation as well as possible. We hope you can find find a
pacific way to solve this problem, however, if you can't don't mistake
several other countries around the world, including Brazil, and I belive
some arabian countries too, is supporting you and almost ready to stay
beside you. You know the American spirit is not living only in US as well
stand by Peter Jordan, this is the spirit that you can see in many other
countries who respect the Democracy, The Human Rights, The Freedom, and have
been proclamed in the France Revolution, US Constitution as well as in many
other constitutions and UN Declarations.
Again, here in Brazil we're deeply touched by your pain and we hope help you
as better as possible to overcome this terrible incident.


Ubirajara Pereira Rodrigues-Filho



From daemon Mon Sep 17 10:09:56 2001



From: John Olesik :      olesik.2-at-osu.edu
Date: Mon, 17 Sep 2001 11:03:39 -0400
Subject: Electron Microscopist Job Opening at Ohio State University

Contents Retrieved from Microscopy Listserver Archives
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Electron Microscopist Position

The Ohio State University Microscopic and chemical Analysis Research Center
(MARC) anticipates an opening for an electron microscopist beginning October
1 to operate a scanning electron microscope (SEM) and an electron microprobe
(EPMA). The position includes working with researchers, teaching students
the basic theory and practical SEM and EPMA measurement techniques,
collaborating on research projects and directing staff assistants. We seek
an excellent microscopist who enjoys the exciting atmosphere of a major
research university and will enjoy collaborating with scientists on a broad
variety of projects. The MARC serves the entire Ohio State University as
well as other institutions, with projects from diverse research areas
including geology, materials science, environmental sciences, biological
and biomedical sciences, dentistry, textiles. The MARC focuses on elemental
analysis using from microscale to bulk analysis using SEM, EPMA, inductively
coupled plasma optical emission spectrometry and inductively coupled mass
spectrometry with laser ablation or solution sampling. The MARC also
teaches traditional or short courses on each of the techniques. The
position is a full time, hard money funded job.

If you know of a suitable candidate for this position, please contact Dr.
John Olesik, MARC Director, at olesik.2-at-osu.edu {mailto:olesik.2-at-osu.edu} or
(614) 292-6954. Excellent candidates with a range of experience and
potential to grow will be considered.

----------------------------------------------------------------------------
--
John Olesik
Adj. Assoc. Professor, Research Scientist
MARC Director
Microscopic and chemical Analysis Research Center
Ohio State University
125 S. Oval Mall
275 Mendenhall Laboratory
Columbus, OH 43210

Office: 026D Mendenhall
Phone: (614) 292-6954
FAX: (614) 292-7688
E-mail: olesik.2-at-osu.edu
Web page: www.geology.ohio-state.edu/marc
----------------------------------------------------------------------------
--



From daemon Mon Sep 17 10:33:56 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Mon, 17 Sep 2001 11:24:56 -0400
Subject: Re: my apology - Thank You!

Contents Retrieved from Microscopy Listserver Archives
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Yes, this is abusing the List. It will be my last abuse, but I could
not leave my inaccurate statement sit.

Thank you for the many emails of support and information.

First. As it was the World Trade Center, along with the large
quantity of Americans, there were many citizens of many countries
that died Tuesday.

Second. Mr. Sinclair was a very real person, and he really did
make those very true statements, albeit back in 1973. If you are
curious, check out:

http://www.rcc.ryerson.ca/schools/rta/ccf/personal/hof/sincla_g.html

Or:

http://www.tysknews.com/Depts/Our_Culture/americans.htm

Darrell



From daemon Mon Sep 17 12:07:57 2001



From: NPGSlithography-at-aol.com
Date: Mon, 17 Sep 2001 12:58:45 EDT
Subject: Re: For the USA

Contents Retrieved from Microscopy Listserver Archives
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Dear Darrell,

The "article" you forwarded is a hoax in that it was originally broadcast in
1973 and the author Gordon Sinclair died in 1984. However, many will agree
that the ideas in the broadcast apply as well today as they did then.

The original version can be read (and heard) at:

http://www.rcc.ryerson.ca/schools/rta/ccf/news/unique/am_text.html

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Mon Sep 17 12:19:04 2001



From: Norman C Miller :      Norman_C_Miller-at-raytheon.com
Date: Mon, 17 Sep 2001 12:14:18 -0500
Subject: used SEM/EDS available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


All,

We have a used Amray 1000A with a PGT IMIX EDS that we will part with, and
are seeking best offer. The equipment details are on the used equipment
page of the MSA web site.

N. Carl Miller


From daemon Mon Sep 17 12:20:26 2001



From: adam.isle-at-hs-scientific.co.uk ()
Date: Mon, 17 Sep 2001 12:16:10 -0500
Subject: Position Opening - Bristol

Contents Retrieved from Microscopy Listserver Archives
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Email: adam.isle-at-hs-scientific.co.uk
Name: Adam Isle

Organization: Hudson Shribman Scientific Recruitment

Location: London, England

I am currently recruiting for a Bristol (South West England) based
high technology company which is seeking an experienced microscopist
to join its pioneering team as a Lab Manager. I would like to know
whether or not you might know of anybody suitably qualified who may
be interested. My client will be happy to arrange Working Visa
details. Regards.

---------------------------------------------------------------------------


From daemon Mon Sep 17 13:06:51 2001



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Mon, 17 Sep 2001 10:59:34 -0700
Subject: RE: SEM imaging on CNT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello Haeseong,
If you are just looking at the nanotubes, it would be easy to place some
of the powder on double-sided carbon tape or double sided Cu tape, and just put
the sample in the SEM. The important thing is to have the machine very well
aligned, and astigmatism corrected for a small spot size and a voltage of about
5kV or less. You should start seeing useable information at a magnifcation
range of 30-50kx and above.

-Brad

----------
From: Haeseong Lee
Sent: Monday, September 17, 2001 5:55 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: SEM imaging on CNT

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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??
Hello.

Could anybody recommend the good method of sample preparation for SEM
imaging on carbon nano tubes?
The sample is a powder type including single wall carbon nanotubes
(SWCNTs).
Thank you very much in advance.

Haeseong Lee

{mailto:haeseong-at-hanyang.ac.kr} haeseong-at-hanyang.ac.kr




From daemon Mon Sep 17 19:02:33 2001



From: Richard Thrift :      Richard_Thrift-at-SkyePharma.com
Date: Mon, 17 Sep 2001 17:24:36 -0700
Subject: Re: general method to extract fat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I seriously doubt that Mr. Jordan meant or implied that there were
terrorists in the Listserver.

Much like the lamentations I hear on the radio seemingly directed at
terrorists about how the terrorists have "awakened a sleeping giant" etc. I
doubt the author meant that everyone listening to the radio is a terrorist.

I am sure it was meant as a public message to vent all of our outrage at
this terrible event.

Regards,


Earl Weltmer


----- Original Message -----
} From: "Alwyn Eades" {jae5-at-lehigh.edu}
To: "EMNET" {microscopy-at-sparc5.microscopy.com}
Sent: Monday, September 17, 2001 5:35 AM


I'm not familiar with recent reviews or specific insect protocols, but there's an older book by Morris Kates called techniques in lipidology -part of a series of lab -type manuals. One of the more universal ways to dissolve / extract lipids is the Bligh-Dyer method (Can J Biochem & Physiol 1959 37:911-917 , paraphrased from Kates:

Extract the specimen with one volume of chloroform:methanol:water 1:2:0.8, which efficiently extracts lipids. the extract is then diluted with one volume each of chloroform and water tp form the two-phased system, chloroform and Methanol/water (1.0:0.9). Any water-soluble contaminants are thus partitioned into the methanol-water phase.

Carbon tet is probably NOT the best to extract lipids with -they are more soluble in chloroform

Richard

} "Vr. Richard Bejsak-Colloredo-Mansfeld" {ricardo-at-ans.com.au} 9/17/01 5:37:30 AM } } }
Dear Colleagues

I like to dissolve fat in insects bodies what could be the best chemical
. benzine, carbon tetrachloride, or???

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld



From daemon Mon Sep 17 19:49:31 2001



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Mon, 17 Sep 2001 19:43:59 -0500
Subject: lanthanum

Contents Retrieved from Microscopy Listserver Archives
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Hello folks. I am trying to fix and stain with lanthanum nitrate and
have followed procedures in Hyatt's book. It mentions that lanthanum
will precipitate in the cold, and when mixed with phosphate buffer.
I have used cacodylate buffer, room temperature solutions and checked
the pH of my buffer(7.2), but I am seeing a lot of ppt in all the
solutions (glut fix, wash and osmium fix). I am wondering if anyone
has any suggestions about how to alleviate this problem. Is the
precipitate harmful to the tissue? Can I filter the solutions to get
rid of big clumps? Is this normal? I would appreciate any feedback
from someone who has used the stuff. Thanks, JoAnn

JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856


From daemon Tue Sep 18 00:40:01 2001



From: Jacob Bastacky :      JBastacky-at-chori.org
Date: Mon, 17 Sep 2001 22:30:31 -0700
Subject: TEM need B&W Print Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Anyone taking down a darkroom or willing to donate a good
photographic processor for black and white prints to a Children's
Hospital?



--
Jacob Bastacky, M.D.
Research Physician
Children's Hospital Oakland Research Institute
5700 Martin Luther King Jr. Way
Oakland, California 94609
Telephone: 510.450.7639
email: jbastacky-at-CHORI.org
FAX: 510.450.7910

Senior Scientist, Visiting
National Center for Electron Microscopy
Lawrence Berkeley National Laboratory
University of California
Berkeley, California 94720
sjbastacky-at-lbl.gov


From daemon Tue Sep 18 04:51:29 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Tue, 18 Sep 2001 11:42:47 +0200
Subject: Quest. about Idefix EDS System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear collegues

I would like to hear some opinions about the Idefix EDS system,
commercialized by SamX in France. Are they (probably french) List Members
who know about it ? Please contact me off-list by mail.

Thanks

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Tue Sep 18 10:54:37 2001



From: Chaoying Ni :      cni-at-udel.edu
Date: Tue, 18 Sep 2001 11:25:58 -0400 (EDT)
Subject: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

Hope you are not knocked off from your normal life by the tragic events.

Anyway, I have a question for you gurus: we have a 2010F TEM sitting in a
regular sized room. Somehow we feel there is a bit above normal acoustic
echos going on in the lab which interferes with the TEM. So we hope to
find some kind of insulation to be put on the wall and/or around the TEM to
improve the performance.

Any thoughts and suggestions are highly appreciated.

Thanks and take care.

*********************************
Chaoying Ni
Electron Microscopy Facility
College of Engineering
University of Delaware
Newark, DE 19716
*********************************



From daemon Tue Sep 18 11:00:26 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 18 Sep 2001 08:49:54 -0700
Subject: Re: TEM of wool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cathy,
When I was at the Australian Electron Microscopy Meeting there were a lot of
papers about wool, including extensive TEM. Perhapps Mel Gibson or someone
at CSIRO (www.csiro.au) could help.
At 09:55 AM 9/17/01 +1000, you wrote:
} Hi,
}
} Can anyone recommend a method for preparing wool from sheep for TEM? Is it
} easy to section?
}
} Thanks
}
} Cathy Gillespie
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Sep 18 11:05:13 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 18 Sep 2001 08:57:18 -0700
Subject: Re: TEM sample prep: Ti extraction replica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Martin,
Although I have not tried an extraction replica on Ti alloys myself, my
Vander Voort lists several Ti alloy etchants, most based on combinations of
HF asnd other strong mineral acids. The first is:
"20 ml HCl
40 ml HF
40 ml H2O
Immerse specimen in solution at 120 - 150 degrees F for 20-30 min. If smut
forms immerse in 30% H2SO4 for 3 min."
The simplest is:
"50 ml HCl
50 ml H2O
General-purpose macroetch (Ogden anbd Holden)."
Hope this helps.
At 06:40 PM 9/17/01 +0800, you wrote:
} Dear all,
}
} I have been given a piece of titanium that contains small
} precipitates of unknown composition and asked to prepare a TEM sample
} so that the precipitates can be analysed. As it is only the
} precipitates that are of interest I thought that the best approach
} would be to make an extraction replica. However, to do that need to
} find a way to etch away the titanium matrix while leaving behind the
} unknown precipitates.
}
} I would be grateful for any recommendations for a suitable etchant
} that will preferentially remove the titanium matrix so that the
} extraction replica can be made.
}
} Regards,
}
} Martin Saunders.
} --
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Sep 18 11:48:58 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Tue, 18 Sep 2001 12:41:47 -0400
Subject: Re: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html






Anyway, I have a question for you gurus: we have a 2010F TEM sitting in a
regular sized room. Somehow we feel there is a bit above normal acoustic
echos going on in the lab which interferes with the TEM. So we hope to
find some kind of insulation to be put on the wall and/or around the TEM to
improve the performance.

Any thoughts and suggestions are highly appreciated.

Dear Chaoying,
One of the other listers will surely know a company which produces
sound-dampening material, but while you wait for it to be delivered, try
using either styrofoam or bubble wrap as a temporary solution. The
styrofoam peanuts would probably work best, but they will take a lot of
labor to install--although you might be able to put up something in front
of the walls and backfill with them. Cloth drapes also work to dampen
sound. Furthermore, you only need to be concerned with the frequency range
which matches resonances in your scope, so when you purchase the
insulation, be sure it will absorb those frequencies. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Tue Sep 18 17:32:13 2001



From: NPGSlithography-at-aol.com
Date: Tue, 18 Sep 2001 18:19:34 EDT
Subject: Re: Sinclair broadcast 1973

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Siegfried,

Your comments about the European aviation industry are certainly true and I
would expect that everyone on the list knows about the widespread use of the
Airbus. Consequently, I did not think it was necessary to explain that I did
not mean that the parts of the radio broadcast which include specific
references to the "American technocracy" which are so clearly outdated should
be considered as applicable today.

I completely agree with you that care must be taken regarding references to
the Sinclair broadcast. In fact, in my original posting, I said "many will
agree", which implies that "not all will agree". Also, I referred to the
"ideas" of the broadcast, not the "specifics" of the broadcast. But as your
e-mail points out, the ambiguity of the "ideas" can still lead to confusion,
since they are left to personal interpretation.

Actually, I hesitated to respond to the original posting at all since it was
clearly off-topic, however, the magnitude of the attack and the historical
foundations of the "hoax", made me think that a short clarification regarding
the original radio broadcast may be of general interest. I certainly did not
mean to imply anything negative regarding the European (or any other
country's) patriotism or spirit and I will be more careful regarding postings
in the future that may have any nationalistic interpretations.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Tue Sep 18 17:33:28 2001



From: Lou Ross :      RossLM-at-missouri.edu
Date: Tue, 18 Sep 2001 17:28:11 -0500
Subject: CSMMS Fall Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


For more information or to register, contact Lou Ross at rosslm-at-missouri or
(573) 882-4777.

Central States Microscopy and Microanalysis Society Fall Meeting
Friday, October 19, 2001
Adams Conference Center
Veterinary Medicine Building
University of Missouri
Columbia, Mo 65211

8 - 8:45 Registration

8:50 Welcome

9:00 Tobias Baskin, University of Missouri-Columbia, "Ultrastructure of the
Plant Cell Wall Analyzed with Field-Emission Scanning Electron Microscopy"

9:15 Nadia Navarrete-Tindall, University of Missouri-Columbia, "Morphology
of the Endangered Legume Amorpha nitens Boynton and its Rhizobia Symbiont"

9:30 Heide Schatten, University of Missouri-Columbia, "Cytoskeletal
Dynamics in Apicomplexan Parasites"

9:45 Joe Simmons, University of Missouri-Columbia, "Idiopathic Interstitial
Pneumonia in Laboratory Rats"

10:00 Marty Katz, University of Missouri-Columbia, "Stem Cell
Transplantation Therapy for Inherited Neurodegenerative Disorders"

10:15 Tom Phillips, University of Missouri-Columbia, "Fixation of
Biological Tissues - Science or Art?"

10:30 - 10:45 BREAK

10:45 Robert Hall, Associate Vice Provost of Research, University of
Missouri-Columbia, "The University of Missouri: Taking the Lead in National
Research Funding"

11:00 Keynote speaker: Kenneth Moore, University of Iowa, MSA Tour Speaker,
"Applications of Microscopy Techniques to the Study of Genetic Therapy
Research"

12:00 - 1:30 Lunch

1:30 John Bozzola, SIU-Carbondale, "Multidisciplinary Approach to Teaching
Electrion Microscopy"

1:45 Howard Wilson, University of Missouri-Columbia, "Creating a Scientific
Poster Presentation using Microsoft Powerpoint"

2:00 Scott Walck, PPG, "Low Angle Cleavage - A New Specimen Preparation
Method for TEM"

2:15 Louis Ross, University of Missouri-Columbia, "Exciting Times in
Scanning Electron Microscopy and X-ray Microanalysis"

2:30 Vladimir Dusevich, University of Missouri-KC, "Practical ESEM"

2:45 Jeff Speakman, University of Missouri-Columbia, "Laser Ablation ICP-MS
as a Tool for Characterization of Archaeological Materials"

3:00 - 3:15 BREAK

3:15 Cammy Bright, University of Missouri-Columbia, "A Laser Ablation
ICP-MS and SEM Study of Rare Earth and Trace Elements in Conodonts"

3:30 Mike Amspoker, Westminster College, "A Light and Scanning Electron
Microscopy Study of the Freshwater Diatom Desmogonium rabenhorstianum
Grunow"

3:45 Heather Ramsay, University of Missouri-Columbia, "New Applications of
SEM in Osteological Morphology Research"

4:00 Dickerson Moreno, University of Missouri, "Development of N-Type
Diamond Semiconductor Through Field Enhanced Diffusion by Optical
Activation"

4:15 Alejandro Suarez, University of Missouri, "A New Method to Fabricate
Polycrystalline Diamond as a P-Type Semiconductor"

4:30 Closing Remarks

4:45 CSMMS Business Meeting







Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.missouri.edu/~geosclmr/ebaf.html


From daemon Tue Sep 18 19:45:27 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 19 Sep 2001 12:32:46 GMT+1200
Subject: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hello, friends

I've just had the misfortune to smash the glass bell jar on my
Edwards 306 coater. About 305mm dia, 400mm high.

While I can and maybe should buy a glass replacement, I'm tempted to
make one from a 400mm length of 305mm diameter stainless-steel pipe
with a flat top of 10mm thick polycarbonate.

I've never liked having my face too close to the glass one when it's
in use, anyway.

Anyone got any comments/recommendations?

Anyone ever had a glass one implode in use?

cheers

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed Sep 19 02:18:49 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 19 Sep 2001 08:15:22 +0100 (GMT Daylight Time)
Subject: Re: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ritchie,

I have not known a glass tube to implode but we use an
outer cover just in case (perspex tube). Tubes are checked
for cracks before use and previous problems have been
obvious, usually by the many bits all over the floor!

My worry would be the 10mm polycarbonate top, at 300mm dia
that's over 1500 pounds (750Kg?) force. I would want
something thicker or stronger.

I also like being able to look in the side to see my
coating set up after evacuation and the evaporation
(through appropriate goggles).

Regards,
Ron

} I've just had the misfortune to smash the glass bell jar on my
} Edwards 306 coater. About 305mm dia, 400mm high.
}
} While I can and maybe should buy a glass replacement, I'm tempted to
} make one from a 400mm length of 305mm diameter stainless-steel pipe
} with a flat top of 10mm thick polycarbonate.
}
} I've never liked having my face too close to the glass one when it's
} in use, anyway.
}
} Anyone got any comments/recommendations?
}
} Anyone ever had a glass one implode in use?
}
} cheers
}
} rtch
}
}
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Sep 19 02:31:44 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 19 Sep 2001 08:34:08 +0100 (GMT Daylight Time)
Subject: Re: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chaoying,

There are two sources of noise, externally and internally
generated. You have specificcally asked about echos so I
assume that your problems stem from the rotary pumps,
cooling fans, etc. on equipment that is essential to have
operating in the room.

Try to get the rotary pumps and power supply rack into an
area that can be screened off from the instrument column
but remember that it will still need air flow for cooling.

For the desk and computer fans use some blackout curtains
around the walls. This is readily available and is usually
black one side and coloured the other so you can have a
nice Oxford blue showing into the room:-) Make sure that it
is at least 1.5 lengths, preferably 2x - ie. 7.5m of
curtaining is a minimum for a 5m wall 10m is best. If you
get the rotary pump and power supply at the back of the
room you can hide them behind 3 layers of the same
curtaining.

We have found this very effective for our HREM instruments
and our FEGTEM.

Noise from outside the room needs dealing with outside the
room.

Regards,
Ron

} Dear Listers,
}
} Hope you are not knocked off from your normal life by the tragic events.
}
} Anyway, I have a question for you gurus: we have a 2010F TEM sitting in a
} regular sized room. Somehow we feel there is a bit above normal acoustic
} echos going on in the lab which interferes with the TEM. So we hope to
} find some kind of insulation to be put on the wall and/or around the TEM to
} improve the performance.
}
} Any thoughts and suggestions are highly appreciated.
}
} Thanks and take care.
}
} *********************************
} Chaoying Ni
} Electron Microscopy Facility
} College of Engineering
} University of Delaware
} Newark, DE 19716
} *********************************
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Sep 19 03:35:21 2001



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Wed, 19 Sep 2001 09:27:07 +0100
Subject: Re: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chaoying,

We have a 2010F in a similar sized room and we use floor to ceiling
black curtaining - the heavier the better (ours are double thickness
and lined) - and it makes a big difference.
Hope this helps
Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365 Mob: 07796 055149
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://borg.shef.ac.uk/fegtem/index.htm
*********************************************


From daemon Wed Sep 19 07:30:15 2001



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 19 Sep 2001 08:18:26 -0400
Subject: Re: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Ritchie,

Don't use a polycarbonate end plate to cover the end of your vacuum
chamber. A flat plate of polycarbonate isn't strong enough to span the
opening.

Edwards did sell glass cylinders with metal endplates on some of their 306
coaters--in particular their old ion beam sputtering system. The unit I
have here has a 1/2 inch thick Aluminum plate spanning a 12 inch diameter
glass vacuum cylinder. The glass cylinder is the same type of glass you
find on a normal bell jar. It just has an L-gasket on both the top and
bottom. Be careful that you use an adequate grade of aluminum for the top
plate.

The advantage of the cylinder with metal top plate is that you can have
feedthroughs to the vacuum chamber from the top as well as through the base
plate.

I suspect that you can get the glass cylindrical glass vacuum vessels and
L-gaskets from the usual vacuum supply houses.

cheers,
Henk Colijn

At 12:32 PM 9/19/01 +0000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Wed Sep 19 09:24:22 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 19 Sep 2001 09:59:59 -0400
Subject: Re: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html






Hi Ritchie,

I have not known a glass tube to implode but we use an
outer cover just in case (perspex tube). Tubes are checked
for cracks before use and previous problems have been
obvious, usually by the many bits all over the floor!

My worry would be the 10mm polycarbonate top, at 300mm dia
that's over 1500 pounds (750Kg?) force. I would want
something thicker or stronger.

I also like being able to look in the side to see my
coating set up after evacuation and the evaporation
(through appropriate goggles).

Regards,
Ron

} I've just had the misfortune to smash the glass bell jar on my
} Edwards 306 coater. About 305mm dia, 400mm high.
}
} While I can and maybe should buy a glass replacement, I'm tempted to
} make one from a 400mm length of 305mm diameter stainless-steel pipe
} with a flat top of 10mm thick polycarbonate.
}
} I've never liked having my face too close to the glass one when it's
} in use, anyway.
}
} Anyone got any comments/recommendations?
}
} Anyone ever had a glass one implode in use?
}
} cheers
}


Dear Ritchie,

I have a polycarbonate lid for a vacuum apparatus with the same
diameter as your coater. It is about 45 mm thick, and it has not
given me any trouble. I agree with Ron that 10 mm is not thick
enough.

Yours,


Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Wed Sep 19 09:55:35 2001



From: Russell E. Cook :      recook-at-anl.gov
Date: Wed, 19 Sep 2001 09:48:53 -0600
Subject: Re: How to insulate noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chaoying:

We've had to contend with noisy rooms in nearly all of our electron
microscope laboratories. Here is a list of things to do:

* Try to eliminate the source of the noise.
* Call in the microscope manufacturer to provide a noise spectrum (dB
versus Hz). Ask which frequencies are a problem (usually low frequencies).
* Look for devices and materials which will attenuate the noise. Pay
particular attention to their effectiveness in attenuating the problem
frequencies.

Here are a few companies which manufacture items you may need:
* Kinetics Noise Control, http://www.kineticsnoise.com
* Industrial Acoustics Company, http://www.industrialacoustics.com
* Industrial Noise Control, http://www.industrialnoisecontrol.com

I've worked several times with David Mitchell, VP at The Huff Co., Lake
Bluff, IL (Chicago area), 1-847-362-7440, to ameliorate our noise problems.
Such companies will evaluate the site, recommend solutions, and even
install the various devices and materials from the manufacturers.

Some of the things we've done are:
* glued "egg-crate" foam on the walls
* installed fabric-covered fiberglass panels on the walls
* installed duct silencers in air ducts
* wrapped air ducts with fiberglass batting backed with "loaded vinyl"
* hung fiberglass noise-absorbing curtains
* installed isolation pads under vibrating equipment

If you install wall panels or foam, it is usually not necessary to cover
all of the wall surfaces. There are lots of things to consider, so
evaluate your site carefully.

..Russ Cook
----------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov

} -----------------------------------------------------------------------.
} Dear Listers,
}
} Hope you are not knocked off from your normal life by the tragic events.
}
} Anyway, I have a question for you gurus: we have a 2010F TEM sitting in a
} regular sized room. Somehow we feel there is a bit above normal acoustic
} echos going on in the lab which interferes with the TEM. So we hope to
} find some kind of insulation to be put on the wall and/or around the TEM to
} improve the performance.
}
} Any thoughts and suggestions are highly appreciated.
}
} Thanks and take care.
}
} *********************************
} Chaoying Ni
} Electron Microscopy Facility
} College of Engineering
} University of Delaware
} Newark, DE 19716
} *********************************





From daemon Wed Sep 19 10:39:15 2001



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 19 Sep 2001 11:26:59 +0200
Subject: Immunogold / using mouse antibodies on mouse tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Is it common to use mouse antibodies (monoclonals) on mouse tissue for
immunogold localizations? Is a control using the secondary antibody
(anti-mouse-IgG-gold-conjugate) alone sufficient to make sure that one
is detecting specificly the primary antibody and not tissue-internal
mouse antibodies?
Does somebody know examples from the literature where people used mouse
antibodies (monoclonals) on mouse tissue for immunogold localizations?
I am a botanist, so until now I never ran into that sort of problems.

Thanks,

Stefan


°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
Universität zu Köln
Botanisches Institut
Gyrhofstr. 15
50931 Köln
Germany
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Wed Sep 19 11:04:57 2001



From: Hank Adams :      hpadams-at-bcm.tmc.edu
Date: Wed, 19 Sep 2001 11:00:52 -0500
Subject: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I didn't get to the MSA meeting this summer, but I heard through the
grapevine about Gatan's new UltraScan 1000 and saw their poster. I was very
impressed with the 4K x 4k montage taken at 5k mag. It looked
publishable.I'd be interested in hearing comments/impressions from anyone
who a chance to use it.
Thanks,

Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030



From daemon Wed Sep 19 11:20:09 2001



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Wed, 19 Sep 2001 12:09:32 -0400
Subject: Re: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Chaoying,

We are about to install a Tecnai 20 TEM. In addition to isolating rotary
pumps and power supply as suggested by Ron Doole, we are covering the walls
with Armstrong Sound Soak Panels.

Frank

At 11:25 AM 9/18/01 -0400, Chaoying Ni wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Sep 19 12:28:57 2001



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 19 Sep 2001 13:17:01 +0200
Subject: Immunogold / using mouse antibodies on mouse tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Is it common to use mouse antibodies (monoclonals) on mouse tissue for
immunogold localizations? Is a control using the secondary antibody
(anti-mouse-IgG-gold-conjugate) alone sufficient to make sure that one
is detecting specificly the primary antibody and not tissue-internal
mouse antibodies? Or are there other things I can doo as a good control?

Does somebody know examples from the literature where people used mouse
antibodies (monoclonals) on mouse tissue for immunogold localizations?
I am a botanist, so until now I never ran into that sort of problems.

Thanks,

Stefan


°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
Universität zu Köln
Botanisches Institut
Gyrhofstr. 15
50931 Köln
Germany
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Wed Sep 19 12:31:33 2001



From: Thimothy Schneider :      timothy.schneider-at-mail.tju.edu
Date: Wed, 19 Sep 2001 13:29:42 -0400
Subject: BELL JAR

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello to the person from down under with the broken bell jar:

I have been using a Denton 502A carbon evaporator for the past decade and
have also had some visions of it imploding. The model I have has a metal
mesh cover over the jar and while it would not stop little pieces of flying
glass it would catch the big ones. As far as using a home made unit goes, I
would be vary cautious. At the very least consult with some kind of
structural engineer who is familiar with vacuum systems. Be careful. Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 JAH
1020 Locust Street
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cell
Timothy.Schneider-at-Mail.TJU.edu



From daemon Wed Sep 19 14:46:30 2001



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Wed, 19 Sep 2001 15:57:40 -0400
Subject: Re: How to insulat(e) noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Sara, actually the total package for the UltraScan 1000 is about $100K. The
UltraScan 4000 is the one you've quoted and that is a 4kx4k camera. The 1000
has the 2kx2k camera and montages the field requiring 4 exposures. I believe
that is the way it works.

Hank

-----Original Message-----
} From: Sara Miller [mailto:saram-at-duke.edu]
Sent: Wednesday, September 19, 2001 1:58 PM
To: Hank Adams


Dear Chaoying,
One unlikely source of noise we recently found came from the
diffusion pump on our CM 300. It was crackling like diffusion pumps
due, but we could see our HRTEM image shake with the big crackles.
The solution was deduced by our excellent FEI field engineer. It was
to reduce the temperature in the diffusion pump by reducing the
amount of oil charge. If you have noise problems that go snap,
crackle, pop in the dark, try checking your oil.
Ciao for now,
Ken


From daemon Wed Sep 19 18:53:38 2001



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Wed, 19 Sep 2001 18:45:24 -0500
Subject: Re: How to insulat(e) noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chaoying,
One unlikely source of noise we recently found came from the
diffusion pump on our CM 300. It was crackling like diffusion pumps
due, but we could see our HRTEM image shake with the big crackles.
The solution was deduced by our excellent FEI field engineer. It was
to reduce the temperature in the diffusion pump by reducing the
amount of oil charge. If you have noise problems that go snap,
crackle, pop in the dark, try checking your oil.
Ciao for now,
Ken


From daemon Wed Sep 19 19:44:56 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 19 Sep 2001 17:39:29 -0700
Subject: Re: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


10 mm polycarbonate? I would suggest much thicker. Have no idea how much
thick. I think the problem is that your pipe has a huge diameter. The
force on your polycarbonate will be proportional to the size of area (1
kg/cm2 x 706 cm2=approx 700 kg, 1600 lb). I afraid, the total cost of the
pipe etc would be comparable to the cost of Bell Jar. You may find Bell
Jar on used equipment sale for about $300.
Sergey

At 12:32 PM 9/19/01 +0000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Sep 19 19:56:52 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 19 Sep 2001 17:51:05 -0700
Subject: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


This is incredible. I would suggest that this is a first EM digital camera
which produces comparable to the film image quality. The chip is huge, so
they have 80-90% area of the film on the chip. The images are very sharp.
The technical problem there was to read information from such huge
chip. They used new technology when chip (virtually?) splitted on quarters
and read in parallel. They did a great job. The camera is very expensive
by the way.

Sergey

At 11:00 AM 9/19/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Sep 19 20:14:55 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 19 Sep 2001 18:09:46 -0700
Subject: RE: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hank,

I think you wrong. They do have two new cameras 2x2K and 4x4K. This is
real number of pixels on the chip. I also believe, Ultrascan1000 is much,
much more expensive than you suggest. As per our conversation with Gatan
people, they expect to start selling that cameras at the end of the year,
so it's very unlikely the price is known for now (I did not ask them
directly about the price, but have impression that it's much more expensive
that previous models, which were in the $70K range). Current Gatan's
cameras (600W/CW Multiscan for instance) already has capacity for multiple
exposure I believe. Actually, it's not a properties of camera but
software. It's called "Auto-montage" and you could "montage" as many
pictures as you want. By the way, Multiscan cameras has 2-4x (not sure in
exact number) bigger area of view than film, which sometime is very
useful. Over last few month I was studying EM digital cameras on the
makket very hard, so if you have questions, may be I could help.

Sergey.

At 02:42 PM 9/19/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Sep 20 06:40:31 2001



From: Andreas Brech :      andreas.brech-at-bio.uio.no
Date: Thu, 20 Sep 2001 13:30:57 +0200
Subject: TEM: fluorescence micrsocopy and TEM on same sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate any advice on the following question. I am planning to do
fluorescence microscopy and electron microscopy on the same sample. I would
like to do immunolabelling on cells grown in a culture dish followed by plastic
embedding of the same sample and further preparation for EM.
In order to find the right area of cells to be sectioned for EM (previously
detected by immuno fluorescence) I suppose one has to have some kind of grid
on the culture dish. I am aware of the fact that there are gridded coverslips
available, however, I always had a lot of trouble to remove the coverslip from
the glass after embedding (used lN2 and so on). So it would be very helpful if
anyone out there could provide any other working recipes, references for
methods or sources for culture dishes with some kind of grid on them.


Dr. Andreas Brech
EM-Unit, Dept. of Biology,
University of Oslo.
P.O.Box 1062 Blindern, Room U 139
Street address: Moltke Moes vei 32
N-0316 Oslo 3
Norway
Tel.: + 47-22 85 47 25/61 89 (work)
+ 47-22 43 83 23 (privat)
Fax.: + 47-22 85 47 26
e-mail.: abrech-at-bio.uio.no


From daemon Thu Sep 20 06:51:53 2001



From: Keith Ryan :      kpr-at-mba.ac.uk
Date: Thu, 20 Sep 2001 12:47:31 +0100
Subject: LM - acridine orange - help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hello Listers

I have a visitor who wants to use acridine orange (0.1%) in seawater (or artificial seawater) to stain nuclei/DNA in limpet eggs and sperm during fertilisation. It is done by other workers, but so far we've found no information as to how long it takes to stain things. Any suggestions? Any pH considerations?

Also, does it work on fresh (i.e. marine, seawater)and formalin-fixed material with equal efficiency.

Finally, we understand that the fluorescence is short-lived - any comments as to how long a time period would be helpful.


Many thanks

Keith Ryan
Marine Biological Association
& University of Plymouth
UK

PLymouth



From daemon Thu Sep 20 07:00:43 2001



From: DrJohnRuss-at-aol.com
Date: Thu, 20 Sep 2001 07:56:12 EDT
Subject: 2nd Announcement: Image Processing and Measurement Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The three-day intensive hands-on workshop on Image Processing and Measurement
presented by John Russ through the North Carolina State University Department
of Continuing and Professional Education is now in its 19th year. The course
will be presented October 30 - November 1, in Raleigh, NC. This course has
generated highly favorable reviews from the thousands of previous students.
The primary focus is on images from various types of microscopy, with
practical guidance in correcting imaging defects, enhancing the images for
presentation and measurement, and performing stereological meaningful
measurements on them. Textbooks and computer software are provided to
attendees. Lab sessions with an opportunity to bring your own images makes
this course immediately useful and highly productive.

For full information on the course, including outlines, faculty information,
a downloadable brochure, and on-line registration, go to

http://members.AOL.com/IPCourse/

or call Cindy Allen, 919-515-8171, at North Carolina State University Dept.
of Continuing and Professional Education.


From daemon Thu Sep 20 11:07:14 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Thu, 20 Sep 2001 10:37:47 -0500
Subject: Enviornmental SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

I am gathering data on enviornmental SEMs. Hopefully I'll be setting up a
research facility within the coming months.

Does anyone have preference to systems?

What, if any differences are there for maintinence compared to a standard
SEM?

COST? (ouch)

Thanks,

Tim Quinn
University of Kansas
Natural History Museum &
Biodiversity Research Center
1345 Jayhawk Blvd 414A
Lawrence, KS 66045
785-864-4556
tquinn-at-ku.edu


From daemon Thu Sep 20 11:39:53 2001



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Thu, 20 Sep 2001 09:37:11 -0700
Subject: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


That *is* incredible.
Chip has length of 4080 pixels by 15 micron pixel pitch = 61.2 mm. Area is then
37.45 square cm or 5.8 square inches. Plate (film) size is 3.25 inches by 4.00
inches = 13 square inches. Chip area is 45% of film area...
-Mike

Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} This is incredible. I would suggest that this is a first EM digital camera
} which produces comparable to the film image quality. The chip is huge, so
} they have 80-90% area of the film on the chip. The images are very sharp.
} The technical problem there was to read information from such huge
} chip. They used new technology when chip (virtually?) splitted on quarters
} and read in parallel. They did a great job. The camera is very expensive
} by the way.
}
} Sergey
}
} At 11:00 AM 9/19/01 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I didn't get to the MSA meeting this summer, but I heard through the
} } grapevine about Gatan's new UltraScan 1000 and saw their poster. I was very
} } impressed with the 4K x 4k montage taken at 5k mag. It looked
} } publishable.I'd be interested in hearing comments/impressions from anyone
} } who a chance to use it.
} } Thanks,
} }
} } Hank Adams
} } Integrated Microscopy Core
} } Molecular and Cellular Biology
} } Baylor College of Medicine
} } Houston, Tx 77030
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu



From daemon Thu Sep 20 12:44:24 2001



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Thu, 20 Sep 2001 13:39:02 -0400
Subject: NYSEM Presidential Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The New York Society of Experimental Microscopists
Announcing the 50th Anniversary
Presidential Symposium

"FRONTIERS in MICROSCOPY"

Caspary Auditorium, Rockefeller University
October 11, 2001 9:00 AM-4:00 PM
Free and open to the Public

50 Years of NYSEM
C. DeLemos-Chiarandini, New York University

George Palade, and the Early Days of Electron Microscopy in New York
P. Satir, Albert Einstein College of Medicine

EM Tomography Reveals Cell Structure at 6nm Resolution
J. R. McIntosh, University of Colorado

Multiphoton Imaging of the Molecular Dynamics of Life Processes
W.Webb, Cornell University

Intravital Imaging of Tumor Cells
J.Condeelis, Albert Einstein College of Medicine

Total Internal Reflectance Microscopy Visualizes Membrane Fusion Events
S. Simon, Rockefeller University

Imaging Biochemistry Inside Cells
P. Bastiaens, EMBL, Heidelberg

The Ribosome: Snapshots of a Molecular Machine in Motion
J. Frank, HHMI, Wadsworth Center

Imaging RNA in Living Cells
R. Singer, Albert Einstein College of Medicine

High Temporal and Spectral Resolution using Acousto-Optical Tunable Filters
D. Farkas, Carnegie-Mellon University

****************************************************************************
Frank Macaluso tel: 718-430-3547
Analytical Imaging Facility fax: 718-430-8996
Albert Einstein College of Medicine e-mail: macaluso-at-aecom.yu.edu
1300 Morris Park Avenue http://www.aecom.yu.edu/aif/
Bronx, NY 10461
****************************************************************************



From daemon Thu Sep 20 12:52:13 2001



From: Gang Ning :      gning-at-mcw.edu
Date: Thu, 20 Sep 2001 12:41:07 -0500
Subject: Re: TEM: fluorescence micrsocopy and TEM on same sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Brech:

You can culture your cells on plastic coverslips and embed them in resin. The
embedded cells are good for both IFM and iEM but you have to work out the
conditions. The coverslip is called Thermanox from EMS.

Greg


Gang Ning, M.D., Ph.D.
Electron Microscopy Facility
Medical College of Wisconsin

Andreas Brech wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I would appreciate any advice on the following question. I am planning to do
} fluorescence microscopy and electron microscopy on the same sample. I would
} like to do immunolabelling on cells grown in a culture dish followed by plastic
} embedding of the same sample and further preparation for EM.
} In order to find the right area of cells to be sectioned for EM (previously
} detected by immuno fluorescence) I suppose one has to have some kind of grid
} on the culture dish. I am aware of the fact that there are gridded coverslips
} available, however, I always had a lot of trouble to remove the coverslip from
} the glass after embedding (used lN2 and so on). So it would be very helpful if
} anyone out there could provide any other working recipes, references for
} methods or sources for culture dishes with some kind of grid on them.
}
} Dr. Andreas Brech
} EM-Unit, Dept. of Biology,
} University of Oslo.
} P.O.Box 1062 Blindern, Room U 139
} Street address: Moltke Moes vei 32
} N-0316 Oslo 3
} Norway
} Tel.: + 47-22 85 47 25/61 89 (work)
} + 47-22 43 83 23 (privat)
} Fax.: + 47-22 85 47 26
} e-mail.: abrech-at-bio.uio.no



From daemon Thu Sep 20 15:17:09 2001



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Thu, 20 Sep 2001 16:08:46 -0400
Subject: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi Listers,
I have a question about a schottky tipped FE Microscope. Specifically, what
is the best way to shut the beam off at the end of the day. We will be using
probably everyday for long sessions, so would beam blanking be preferable to
shutting off the extraction voltage. I am wondering about the effects of tip
life, beam stability, and contamination. Any advice or past experience would
be greatly appreciated.
Thanks again
Nick Aitken

Nicol Aitken
Sample Preparation and Imaging Specialist
Research and Development
Semiconductor Insights Inc.
email:nicol-at-semiconductor.com
(613)599-6500 ext.4300


From daemon Thu Sep 20 16:47:12 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 20 Sep 2001 14:41:01 -0700
Subject: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ingo

Yes, I did check your WEB-site in the beginning of my search a few month
ago as well as many others. My initial criteria were the following:

-35 mm port mount;
-price;
-ability to have demo for the camera;
-ability to have reasonable service in time.

You product was excluded from initial list for the following reasons:

- your Biocam camera, which was possible candidate is bottom-mounted;

- on your WEB-site at http://www.tvips.com/Contact_en.htm I did not find
any information about your headquarter in US or contact phones, so I was
not able to talk to your representative (I was using the telephone call as
a first "try" of the customer service quality: if I was on hold for more
than 5 min, I excluded the candidate from my list).

-location of your head office in Germany makes me skeptical about customer
service quality company could provide in US.

I would like to mention that my criteria is subjective and based on my own
way to make a business and spent my money. My decision is not related to
the real quality of your products.

I would mention also that a few companies refused to give me demo on their
cameras (or delayed with answer) and were excluded from the list as well.

Sergey.

At 02:30 PM 9/20/01 +0200, you wrote:
} Dear Mr. Sergey Ryazantsev,
}
} I have read in your email, that you have studied cameras for TEM.
} Have you studied our cameras as well?
}
} For example, this year we have had the following installations in California:
} - Scripps in San Diego, F-224 at a CM-200FEG (Ron Milligan's lab)
} - UC in San Diego, F-224/FastScan at a JEM-200EX (Mark Ellisman's lab)
} - Quantum Dot Corporation in Hayward, TemCam-0124 at JEM-200CX
} - Additionally just this month Bridget Carragher (together with her group) has
} moved from Illinois to the Scripps Institute. She will mount another F-224
} at a
} Tecnai-20FEG.
}
} If you want to know more about our US installations and our cameras,
} please let
} me know.
}
} Best wishes,
} Ingo Daberkow
}
} BTW: We are in contact with Phoebe Stewart from the Department of Molecular &
} Medical Pharmacology, UCLA School of Medicine
}
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} Dr. Ingo Daberkow
} Tietz Video and Image Processing Systems GmbH
} Herbststrasse 7
} D-82131 Gauting, Germany
} Tel: +49-89-8506567
} FAX: +49-89-8509488
} Internet: http://www.tvips.com/
} Email: ingo.daberkow-at-tvips.com
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
}
} Sergey Ryazantsev wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hank,
} }
} } I think you wrong. They do have two new cameras 2x2K and 4x4K. This is
} } real number of pixels on the chip. I also believe, Ultrascan1000 is much,
} } much more expensive than you suggest. As per our conversation with Gatan
} } people, they expect to start selling that cameras at the end of the year,
} } so it's very unlikely the price is known for now (I did not ask them
} } directly about the price, but have impression that it's much more expensive
} } that previous models, which were in the $70K range). Current Gatan's
} } cameras (600W/CW Multiscan for instance) already has capacity for multiple
} } exposure I believe. Actually, it's not a properties of camera but
} } software. It's called "Auto-montage" and you could "montage" as many
} } pictures as you want. By the way, Multiscan cameras has 2-4x (not sure in
} } exact number) bigger area of view than film, which sometime is very
} } useful. Over last few month I was studying EM digital cameras on the
} } makket very hard, so if you have questions, may be I could help.
} }
} } Sergey.
} }
} } At 02:42 PM 9/19/01 -0500, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Sara, actually the total package for the UltraScan 1000 is about
} $100K. The
} } } UltraScan 4000 is the one you've quoted and that is a 4kx4k camera.
} The 1000
} } } has the 2kx2k camera and montages the field requiring 4 exposures. I
} believe
} } } that is the way it works.
} } }
} } } Hank
} } }
} } } -----Original Message-----
} } } } From: Sara Miller [mailto:saram-at-duke.edu]
} } } Sent: Wednesday, September 19, 2001 1:58 PM
} } } To: Hank Adams
} } } Subject: Re: Gatan's new UltraScan 1000
} } }
} } }
} } } Micrograph was lovely. Price tag was in range of $275 K--ouch! They
} didn't
} } } have one there on demo that I saw.
} } }
} } } Sara E. Miller, Ph. D.
} } } P. O. Box 3712
} } } Duke University Medical Center
} } } Durham, NC 27710
} } } Ph: 919 684-3452
} } } FAX: 919 684-3265
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Sep 20 16:47:48 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 20 Sep 2001 14:44:00 -0700
Subject: RE: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hank
Thanks for the comment.

Steve Pfeiffer of Gatan just E.mail to me that price range for Ultrascan
1000 is about $100K. Which is much less than I expect to see for such camera.

Sergey

At 10:06 AM 9/20/01 -0500, you wrote:
} Sergey, I just talked to Gatan about their UltraScan 1000 that tiles 4
} images together. The entire package with installation/training and the
} Automontage plug-in minus the computer/monitor is about $100K. Using their
} specs for the computer add another $4800 which includes a 21" monitor. The
} CCD magnifies the image 1.3x-1.5x compared to film.
}
} Hank
} -----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Wednesday, September 19, 2001 8:10 PM
} To: Hank Adams; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Gatan's new UltraScan 1000
}
}
} Hank,
}
} I think you wrong. They do have two new cameras 2x2K and 4x4K. This is
} real number of pixels on the chip. I also believe, Ultrascan1000 is much,
} much more expensive than you suggest. As per our conversation with Gatan
} people, they expect to start selling that cameras at the end of the year,
} so it's very unlikely the price is known for now (I did not ask them
} directly about the price, but have impression that it's much more expensive
} that previous models, which were in the $70K range). Current Gatan's
} cameras (600W/CW Multiscan for instance) already has capacity for multiple
} exposure I believe. Actually, it's not a properties of camera but
} software. It's called "Auto-montage" and you could "montage" as many
} pictures as you want. By the way, Multiscan cameras has 2-4x (not sure in
} exact number) bigger area of view than film, which sometime is very
} useful. Over last few month I was studying EM digital cameras on the
} makket very hard, so if you have questions, may be I could help.
}
} Sergey.
}
} At 02:42 PM 9/19/01 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Sara, actually the total package for the UltraScan 1000 is about $100K. The
} } UltraScan 4000 is the one you've quoted and that is a 4kx4k camera. The
} 1000
} } has the 2kx2k camera and montages the field requiring 4 exposures. I
} believe
} } that is the way it works.
} }
} } Hank
} }
} } -----Original Message-----
} } } From: Sara Miller [mailto:saram-at-duke.edu]
} } Sent: Wednesday, September 19, 2001 1:58 PM
} } To: Hank Adams
} } Subject: Re: Gatan's new UltraScan 1000
} }
} }
} } Micrograph was lovely. Price tag was in range of $275 K--ouch! They
} didn't
} } have one there on demo that I saw.
} }
} } Sara E. Miller, Ph. D.
} } P. O. Box 3712
} } Duke University Medical Center
} } Durham, NC 27710
} } Ph: 919 684-3452
} } FAX: 919 684-3265
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Sep 20 17:04:22 2001



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 20 Sep 2001 17:59:41 -0400
Subject: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Nicol,

For the past 4 years we have run the thermal emitter in our Zeiss LEO
DSM982 almost everyday Monday-Friday and off on weekends and vacations. The
previous emitter (Zeiss OEM) lasted 14,900 hours over 3 years. The present
emitter (from FEI) has 3,000 hours since November 2000. We routinely image
at } 100kx with great results.
What microscope do you have?

Jim







Nicol Aitken wrote:

Hi Listers,
I have a question about a schottky tipped FE Microscope. Specifically, what
is the best way to shut the beam off at the end of the day. We will be using
probably everyday for long sessions, so would beam blanking be preferable to
shutting off the extraction voltage. I am wondering about the effects of tip
life, beam stability, and contamination. Any advice or past experience would
be greatly appreciated.
Thanks again
Nick Aitken

Nicol Aitken
Sample Preparation and Imaging Specialist
Research and Development
Semiconductor Insights Inc.
email:nicol-at-semiconductor.com
(613)599-6500 ext.4300

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Fri Sep 21 06:00:29 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 21 Sep 2001 11:58:12 +0100 (GMT Daylight Time)
Subject: Re: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Nicol and Jim,

There may be a difference in TEM and SEM FEG use that I am
not familiar with, I am primarily a TEM guy, however we
keep our emission running as much as possible.

With our JEOL3000F FEGTEM we run the tip down maybe 4 times
a year, other than that it is on and the valve between the
gun and the column is closed when the instrument is not
being used. There are several reasons for this.

It takes a couple of hours to run up and condition the HT
at 300kV and then another couple of hours to run up the
emission. This makes it impossible to switch on the
emission for a day's use.

Having run up the emission it is not stable, the emission
current keeps dropping. This exponential decrease takes
several hours to stabilise (we allow 6 hours before we
think it is really stable). Of course if you don't need a
stable current (eg. for HREM imaging) you can still use it.

The most likely time for any damage to the tip (apart fom
accidents) is when it is heated or cooled so we don't want
to do that more than neccessary.

I guess that there is finite life of the tip so it would be
pointless to keep it running for a long time if the
instrument is not being used. Does anyone know why a tip
should eventually fail if operating conditions are
optimised?

Our present tip (the first) has been running during factory
tests and since installation in Oxford in 1998 and has run
for over 21,500 hours.

Regards,
Ron
ps. If our tip now fails I'm going to blame you guys for
raising the subject.



} Nicol,
}
} For the past 4 years we have run the thermal emitter in our Zeiss LEO
} DSM982 almost everyday Monday-Friday and off on weekends and vacations. The
} previous emitter (Zeiss OEM) lasted 14,900 hours over 3 years. The present
} emitter (from FEI) has 3,000 hours since November 2000. We routinely image
} at } 100kx with great results.
} What microscope do you have?
}
} Jim
}
}
} Hi Listers,
} I have a question about a schottky tipped FE Microscope. Specifically, what
} is the best way to shut the beam off at the end of the day. We will be using
} probably everyday for long sessions, so would beam blanking be preferable to
} shutting off the extraction voltage. I am wondering about the effects of tip
} life, beam stability, and contamination. Any advice or past experience would
} be greatly appreciated.
} Thanks again
} Nick Aitken
}
} Nicol Aitken
} Sample Preparation and Imaging Specialist
} Research and Development
} Semiconductor Insights Inc.
} email:nicol-at-semiconductor.com
} (613)599-6500 ext.4300
}
} James S. Romanow
} The University of Connecticut
} Physiology and Neurobiology Department
} Electron Microscopy Facility
} Unit-2131
} Storrs, CT 06269-2131
} bsgphy3-at-uconnvm.uconn.edu
} 860 486-2914 voice
} 860 486-1936 fax
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri Sep 21 07:33:02 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 21 Sep 2001 08:23:56 -0400
Subject: RE: LM - acridine orange - help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Morning (for me!) Keith,

For the most complete information on fluorescence probes (which you may
already know), try URL: http://www.probes.com/; Search: "Acridine Orange"
for a plethora (my son accused me of a "plethora of harassment", the other
night!) of information and citations.

You might also consult Hayat, MA, Stains and Cytochemical Methods, Plenum,
1993, ISBN: 0-306-44294-9 for an informative, though not explicit,
description of AO and its applications and properties.

For the reason that AO has been used extensively in flow cytometry, you
should also look into the several chapters that are relevant to your
question in: Methods in Cell Biology, vol 33, Flow Cytometry, Darzynkiewicz
and Crissman(Eds.)Academic Press, 1990, ISBN: 0-12-564133-8 or
0-12-203050-8.

pH is important to the fluorescent properties of the dissolved dye, but I
have not observed such effects in my experience which has been confined to
viewing virus-infected cultured cells (vital, but usually fixed/dead). My
original histochemical use of AO fluorescence - early '60's - was to
distinguish between DNA and RNA - until it was pointed out by virologists
that there were some single-stranded DNA viruses that could interfere with
interpretation. Now we know that AO can be used to intercalate
preferentially in transcribing DNA, thus providing an indication of RNA
production.

I hope this helps, and

Good luck,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



} ----------
} From: Keith Ryan
} Reply To: kpr-at-mba.ac.uk
} Sent: Thursday, September 20, 2001 7:47 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM - acridine orange - help
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello Listers
}
} I have a visitor who wants to use acridine orange (0.1%) in seawater (or
} artificial seawater) to stain nuclei/DNA in limpet eggs and sperm during
} fertilisation. It is done by other workers, but so far we've found no
} information as to how long it takes to stain things. Any suggestions? Any
} pH considerations?
}
} Also, does it work on fresh (i.e. marine, seawater)and formalin-fixed
} material with equal efficiency.
}
} Finally, we understand that the fluorescence is short-lived - any comments
} as to how long a time period would be helpful.
}
}
} Many thanks
}
} Keith Ryan
} Marine Biological Association
} & University of Plymouth
} UK
}
} PLymouth
}
}
}


From daemon Fri Sep 21 08:05:28 2001



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 21 Sep 2001 08:51:01 -0400
Subject: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


hi-

my experience is the same as jim's. i've got about 10Khours on an fei
emitter now...still works great at } 500KX. its "on" all week and "off"
most weekends.

b-


} X-Comment: UConnVM.UConn.Edu: Mail was sent by d40h44.public.uconn.edu
} Date: Thu, 20 Sep 2001 17:59:41 -0400
} To: Microscopy-at-sparc5.microscopy.com
} From: Jim Romanow {bsgphy3-at-uconnvm.uconn.edu}
} Subject: Thermal FE
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Fri Sep 21 08:49:40 2001



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Fri, 21 Sep 2001 15:41:32 +0200
Subject: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Sergey,

Thanks for the fast reaction, *but* you have forwarded my direct email to you to
the listserver as well.

Do you really want to discuss this matter at the listserver?

Of course, I am willing to discuss the advantage/disadvantage of our cameras in
publicity, buy I fear this might be regarded as advertisement!?

Therefore, at first, I want to know the opinion of the list server community!
Nestor, maybe do you can comment it?

Best wishes,
Ingo


Sergey Ryazantsev wrote:

} Dear Ingo
}
} Yes, I did check your WEB-site in the beginning of my search a few month
} ago as well as many others. My initial criteria were the following:
}
} -35 mm port mount;
} -price;
} -ability to have demo for the camera;
} -ability to have reasonable service in time.
}
} You product was excluded from initial list for the following reasons:
}
} - your Biocam camera, which was possible candidate is bottom-mounted;
}
} - on your WEB-site at http://www.tvips.com/Contact_en.htm I did not find
} any information about your headquarter in US or contact phones, so I was
} not able to talk to your representative (I was using the telephone call as
} a first "try" of the customer service quality: if I was on hold for more
} than 5 min, I excluded the candidate from my list).
}
} -location of your head office in Germany makes me skeptical about customer
} service quality company could provide in US.
}
} I would like to mention that my criteria is subjective and based on my own
} way to make a business and spent my money. My decision is not related to
} the real quality of your products.
}
} I would mention also that a few companies refused to give me demo on their
} cameras (or delayed with answer) and were excluded from the list as well.
}
} Sergey.
}



From daemon Fri Sep 21 08:57:45 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 21 Sep 2001 14:52:55 +0100 (GMT Daylight Time)
Subject: Re: Enviornmental SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Only the FEI ESEM allows you to have water in the chamber.
If I had the money I would get the FEGESEM.

Re maintainance, we do not have any extra problems with our
XL30 ESEM as our wet bullet apertures are replaced at
service. Our contract gives us 4 services a year. It would
be useful to get comments from a user without a service
contract.

Dave



On Thu, 20 Sep
2001 10:37:47 -0500 "Quinn, Tim Lee" {tquinn-at-ku.edu} wrote:

}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
Society of America } To Subscribe/Unsubscribe -- Send
Email to ListServer-at-MSA.Microscopy.Com } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
} } Dear Listservers,
} } I am gathering data on enviornmental SEMs. Hopefully
I'll be setting up a } research facility within the coming
months. }
} Does anyone have preference to systems? }
} What, if any differences are there for maintinence
compared to a standard } SEM?
} } COST? (ouch)
} } Thanks,
} } Tim Quinn
} University of Kansas } Natural History Museum &
} Biodiversity Research Center } 1345 Jayhawk Blvd 414A
} Lawrence, KS 66045 } 785-864-4556
} tquinn-at-ku.edu }

----------------------------------------
Patton, David Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Sep 21 10:10:32 2001



From: Michelle_M_Adams-at-Brown.EDU (Michelle Adams)
Date: Fri, 21 Sep 2001 11:01:08 -0400
Subject: Immungold question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in subscribing to the microscopy listserver.

I have a question about immunogold labeling.
I am interested in surface labeling receptors and examining patterns of
internalization.
Has anyone tried to label the surface receptor with a primary antibody in
the live cell, induce internalization, fix and embed the cells. Then thin
section the preparation and do postembedding with a secondary antibody
tagged with a gold particle? Do you know if the primary antibody will
survive the embedding process with low-termperature embedding with
lowicryl?
Thanks for your help.
Michelle Adams




From daemon Fri Sep 21 10:29:33 2001



From: Christopher Gilpin :      Christopher.Gilpin-at-utsouthwestern.edu
Date: Fri, 21 Sep 2001 10:22:54 -0500
Subject: Field Cancelling systems and Mu Metal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I hope to be installing a new TEM soon and unfortunately have some concerns about magnetic field interference with the column.

Being a new "immigrant" to the USA I am not up to speed with vendors on this side of the Atlantic.
I need to know who supplies field canceling systems in the USA - calls from vendors welcome. I would also be interested in colleagues experiences with individual systems. I may have to deal with particularly high field strengths in the vicinity of the microscope so would be interested in what is the maximum field strength that such systems can compensate.
Another alternative would be to look into Mu metal lining for the microscope room. Again info on vendors would be most useful as well as anyone's experience of using this method of shielding.

Many thanks


Chris



Christopher Gilpin Ph.D.
Assistant Professor
Director, Molecular and Cellular Imaging Facility
Cell Biology Department
University of Texas Southwestern Medical Center at Dallas
Dallas, TX, 75390-9039
phone +1 214 648 2827
fax +1 214 648 6408
christopher.gilpin-at-utsouthwestern.edu



From daemon Fri Sep 21 10:57:48 2001



From: Glenn Fried :      gfried-at-uiuc.edu
Date: Fri, 21 Sep 2001 10:53:51 -0500
Subject: Fwd: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On a related note we just purchased a camera from Ingo. We have been
extremely happy with the performance. The camera was installed about one
mouth after tvips received the purchase order and the instating went very
smoothly. If anyone has questions please contact me.

Glenn




} Sergey,
}
} Thanks for the fast reaction, *but* you have forwarded my direct email to
} you to
} the listserver as well.
}
} Do you really want to discuss this matter at the listserver?
}
} Of course, I am willing to discuss the advantage/disadvantage of our
} cameras in
} publicity, buy I fear this might be regarded as advertisement!?
}
} Therefore, at first, I want to know the opinion of the list server community!
} Nestor, maybe do you can comment it?
}
} Best wishes,
} Ingo
}
}
} Sergey Ryazantsev wrote:
}
} } Dear Ingo
} }
} } Yes, I did check your WEB-site in the beginning of my search a few month
} } ago as well as many others. My initial criteria were the following:
} }
} } -35 mm port mount;
} } -price;
} } -ability to have demo for the camera;
} } -ability to have reasonable service in time.
} }
} } You product was excluded from initial list for the following reasons:
} }
} } - your Biocam camera, which was possible candidate is bottom-mounted;
} }
} } - on your WEB-site at http://www.tvips.com/Contact_en.htm I did not find
} } any information about your headquarter in US or contact phones, so I was
} } not able to talk to your representative (I was using the telephone call as
} } a first "try" of the customer service quality: if I was on hold for more
} } than 5 min, I excluded the candidate from my list).
} }
} } -location of your head office in Germany makes me skeptical about customer
} } service quality company could provide in US.
} }
} } I would like to mention that my criteria is subjective and based on my own
} } way to make a business and spent my money. My decision is not related to
} } the real quality of your products.
} }
} } I would mention also that a few companies refused to give me demo on their
} } cameras (or delayed with answer) and were excluded from the list as well.
} }
} } Sergey.
} }

Glenn Fried
Imaging Technology Group
Beckman Institute University of Illinois
phone 217 333 5493
Fax 217 244 6219



From daemon Fri Sep 21 12:56:45 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 21 Sep 2001 12:41:34 -0500
Subject: RE: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I shut extraction voltage off only when
I was going on a long vacation and when I was told
there could be a possibility of power outage (I put a
microscope in a stand-by mode). Thermo cycling is the
second worst thing you can do to the tip after
power outage.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Nicol Aitken [mailto:nicol-at-semiconductor.com]
} Sent: Thursday, September 20, 2001 3:09 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Thermal FE
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} Hi Listers,
} I have a question about a schottky tipped FE Microscope.
} Specifically, what
} is the best way to shut the beam off at the end of the day.
} We will be using
} probably everyday for long sessions, so would beam blanking
} be preferable to
} shutting off the extraction voltage. I am wondering about the
} effects of tip
} life, beam stability, and contamination. Any advice or past
} experience would
} be greatly appreciated.
} Thanks again
} Nick Aitken
}
} Nicol Aitken
} Sample Preparation and Imaging Specialist
} Research and Development
} Semiconductor Insights Inc.
} email:nicol-at-semiconductor.com
} (613)599-6500 ext.4300
}
}
}


From daemon Fri Sep 21 16:12:35 2001



From: Brian J Laughlin :      brjlau18-at-US.ibm.com
Date: Fri, 21 Sep 2001 17:02:33 -0400
Subject: Cron deamon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have been getting mail from root-at-mail.advancelink.com all afternoon.
Twenty or more of these emails. Other (I think that are on this list) have
been getting the same email but from my email address. I have not sent
these messages.

I have this bad feeling that this is a virus and it I have gotten from the
listserver somehow. Could the admin of this listserver check this out.

I am sorry if I am making false accusations.

Sincerely,

Brian Laughlin

FIB/TEM Engineer
IBM, Burlington, VT
Microelectronics Division
Surface and Materials Science Laboratory (Dept. GP8)


Lab: Bldg. 967-1 N18, (802) 769-1596
Fax: (802) 769-1220
Mail: IBM Burlington, 1000 River St., Essex Junction, VT 05452 Mailstop
967L




From daemon Fri Sep 21 17:34:36 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 21 Sep 2001 15:18:35 -0700
Subject: Re: Thermal FE discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


What's the rationale for turning it off over weekends?

My system uses, I believe, a Denka ZrO/W filament in my
Amray 305FE gun assembly. I never shut the gun off.
The main problem with this particular SEM is that the
gun valve is manual. If the gun is on and the gun valve
is closed, there is most likely to be an air burp into the
gun chamber and will poison the filament. This process
also typically will burn the valves O-rings due to the high
intensity FE emitter beam. So the valve is never closed
until after turning off the filament.

The only reason I have found to worry about closing the
gun valve is if there is a power failure. That would shut
down the filament and all pumps (mech, turbo, column and
gun ion pumps). I solved this by using two Toshiba 1400XL+
6KVA dual conversion UPS units. Now, I shut the console
off but keep it leaves the pumps and gun running. Plus, the
UPS units

I log gun and column vacuum every few days along with
extractor current. Then, about every month, I measure
gun brightness. Running the way I am now, brightness
is +- no more than about 0.4nA. Iext may change 3-6uA
but brightness is way less affected by changes in Iext.
When Iext and brightness start to drop or waver, that is
an onerous sign that the gun is starting to fail.

I would tend to stick with this pattern since I know that
bringing up a new gun takes about a week for the
Iext and brightness to stabilize. I suspect that there would
be some proportional time to stabilize if shut down over
a weekend or for some extended time. Since I don't use
the SEM every day, if shutting off the filament during some
periods of time would extend its life, that is very desirable.

I have 9,020 hours on this gun since newly installed.
It is very stable.

gary g.

At 05:51 AM 9/21/2001, you wrote:

} hi-
}
} my experience is the same as jim's. i've got about 10Khours on an fei
} emitter now...still works great at } 500KX. its "on" all week and "off"
} most weekends.
}
} b-
}
}
} } X-Comment: UConnVM.UConn.Edu: Mail was sent by d40h44.public.uconn.edu
} } Date: Thu, 20 Sep 2001 17:59:41 -0400
} } To: Microscopy-at-sparc5.microscopy.com
} } From: Jim Romanow {bsgphy3-at-uconnvm.uconn.edu}
} } Subject: Thermal FE
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Nicol,
} }
} } For the past 4 years we have run the thermal emitter in our Zeiss LEO
} } DSM982 almost everyday Monday-Friday and off on weekends and vacations. The
} } previous emitter (Zeiss OEM) lasted 14,900 hours over 3 years. The present
} } emitter (from FEI) has 3,000 hours since November 2000. We routinely image
} } at } 100kx with great results.
} } What microscope do you have?
} }
} } Jim
} }
} }
} }
} }
} }
} }
} }
} } Nicol Aitken wrote:
} }
} } Hi Listers,
} } I have a question about a schottky tipped FE Microscope. Specifically, what
} } is the best way to shut the beam off at the end of the day. We will be using
} } probably everyday for long sessions, so would beam blanking be preferable to
} } shutting off the extraction voltage. I am wondering about the effects of tip
} } life, beam stability, and contamination. Any advice or past experience would
} } be greatly appreciated.
} } Thanks again
} } Nick Aitken
} }
} } Nicol Aitken
} } Sample Preparation and Imaging Specialist
} } Research and Development
} } Semiconductor Insights Inc.
} } email:nicol-at-semiconductor.com
} } (613)599-6500 ext.4300
} }
} } James S. Romanow
} } The University of Connecticut
} } Physiology and Neurobiology Department
} } Electron Microscopy Facility
} } Unit-2131
} } Storrs, CT 06269-2131
} } bsgphy3-at-uconnvm.uconn.edu
} } 860 486-2914 voice
} } 860 486-1936 fax
} }
} }
} }
} }
} }
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875



From daemon Fri Sep 21 18:29:07 2001



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Fri, 21 Sep 2001 17:21:57 -0600
Subject: Re: Random mail from your server to me

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I received this reply from a person about the cron daemon problem this
afternoon. If anyone has continuing problems, I suggest you contact them
directly.

--John
chandler-at-colostate.edu
Colorado State University



} From: "Kim M. Shiu" {kmshiu-at-mail.advancelink.com}
} Subject: Re: Random mail from your server to me
} To: chandler-at-lamar.colostate.edu (John Chandler)
} Date: Fri, 21 Sep 101 16:23:52 -0700 (PDT)
} MIME-Version: 1.0
} Status: RO
}
} Hi,
}
} There was a problem with a program on our server. It was not intentional
} to send multiple copies or spam anyone. The problem has been corrected
} and we apologize for any inconvenience it caused.
}
} Thanks.
}
} Kim Shiu
} Advancelink
}
} }
} } I sent this message to postmaster-at-advancelink.com and the messages continue
} } to be sent. Please attend to this immediately.
} }
} } --John
} }
} } FYI, I have receive 7 messages from your mail server this afternoon similar
} } or identical to the one below. This might indicate a situation that
} } requires your attention.
} }
} } John Chandler
} } Colorado State University
} } chandler-at-lamar.colostate.edu
} }
} }
} }
} } ============================================================================== }
} } }
} } Received: from mail.advancelink.com ([207.214.173.11]) by
} } lamar.ColoState.EDU (AIX4.3/8.9.3/8.8.8) with ESMTP id NAA237230 for
} } {john.chandler-at-colostate.edu} ; Fri, 21 Sep 2001 13:28:56 -0600
} } Received: (from root-at-localhost)
} } by mail.advancelink.com (8.8.5/8.8.5) id LAA29356;
} } Fri, 21 Sep 2001 11:35:00 -0700 (PDT)
} } Date: Fri, 21 Sep 2001 11:35:00 -0700 (PDT)
} } Message-Id: {200109211835.LAA29356-at-mail.advancelink.com}
} } From: root-at-mail.advancelink.com (Cron Daemon)
} } To: coccust-at-mail.advancelink.com
} } Subject: Cron {coccust-at-mail} csh -x ~/bin/get-dist } get-dist.log
} } X-Cron-Env: {SHELL=/bin/sh}
} } X-Cron-Env: {HOME=/usr/home/coc/coccust}
} } X-Cron-Env: {LOGNAME=coccust}
} } X-Cron-Env: {USER=coccust}
} } Status:
} }
} } cd /usr/home/coc/coccust
} } grep [a-z] /usr/home/coc/cocinfo/MAILING-LISTS/CURRENT-LIST.txt
} } chown coccust .forward
} } chown: Command not found.
} } chown coc .forward
} } chown: Command not found.
} } chmod 664 .forward
} } exit
} }
} }
} }
}




From daemon Sat Sep 22 11:06:21 2001



From: Karl Garsha :      keg-at-csd.uwm.edu
Date: Sat, 22 Sep 2001 11:03:05 -0500
Subject: Re: Immunogold / using mouse antibodies on mouse tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Stefan,
My initial reaction is that it is not common to use murine (mouse)
monoclonal antibodies on mouse tissue. The reason for this is that if these
murine antibody hybridomas were derived from mouse B-cells, and these
B-cells produced antibodies to a mouse protein, one would expect an
autoimmune response in the original mouse. In other words, it not be
logical to produce an antibody against mouse proteins in a mouse because
either: 1.) a secondary immune response would not be observed, or 2.) the
ensuing autoimmune response would kill or at least severly debilitate the
mouse. I guess recombinant monoclonal murine antibodies against mouse
proteins could be produced however. A more likely scenario is that these
mouse monoclonal antibodies are against a protein antigen derived from
another species.
A couple of important controls are in order. The simplest is to assess
non-specific binding of your secondary (gold-conjugate) antibody. To do
this you might block your tissue as usual, apply PBS or murine pre-immune
sera to the tissue in place of the primary antibody, and then apply your
secondary antibody.
Another important control is to asses non-specific binding of your
primary antibody. This is an isotype control. You should be able to find
out the isotype of your monoclonal antibody from the source (eg. IgG K2B).
Then obtain a monoclonal antibody of the same isotype which is not specific
for any mouse protein (eg. anti-dansyl or something). This is used in place
of the mouse specific monoclonal to assess non-specific binding due to
charge properties. An isotype control for the secondary antibody could be
conducted as well to assess the presence of tissue internal antibodies.
-Karl G.
_________________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
Room B650J
University of Illinois at Urbana-Champaign
Urbana, IL 61801
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Sat Sep 22 11:29:25 2001



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Sat, 22 Sep 2001 11:32:31 -0500
Subject: fouled objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers,
As someone new to the challenge of managing multiuser equipment, I have
come to realize that it will be important to devise an effective strategy to
minimize wear and tear on instrumentation. It will also be important to
attempt to try to salvage the results of the inevetable mistakes that users
make.
I am hoping some of the seasoned veterans out there can offer some
advice. My question concerns how best to try to clean non-oil objectives
which have been used with oil. I was previously under the impression that
attempting this was futile, however I am hoping that I'm wrong. A collegue
of mine suggested sonicating in acetone for a couple of minutes, but I'm
hoping a gentler approach will suffice. Would the use o