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Raj, was the cryopump made by Balzers? If not, I suspect it was made by CTI
Cryogenics - try www.ctivacuum.com for parts, service, or replacement. I
don't know for sure if they have used/rebuilt - they may. Also try
www.bidservice.com for used cryopumps and Helium compressors.

No interest in either one, just passing the information along.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Raj Patel {rpatel-at-umdnj.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 01, 2002 12:56 PM

Earl, try these:

1) Malin Co., 5400 Smith Road, Brook Park, OH 44142. Specialty wire. Tel.
(216)267-9080. I don't know if they have a web site.
2) Alfa Aesar, www.alfa.com , (800)343-0660, and (800)343-7276 for tech
support.
3) Little Falls Alloys, www.lfa-wire.com , (888)5329473 or (973)278-1666.

Alfa will probably have the purest Copper, as far as Oxygen and other
impurities go (the best is 99,9999% metals basis). Little Falls Alloys may
have more suitable specialty Copper alloy, especially for high temperature
apps.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Earl Weltmer (by way of MicroscopyListserver) {earlw-at-sbcglobal.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 01, 2002 7:36 PM

Mary
Isn't this backwards? Oxygenated blood appears redder in
transmitted or reflected light because it absorbs *blue*, and
transmits or reflects the remaining red.
} Basically, oxygen saturation is measured by
} passing 2 light beams through the finger, one red light (660 nm), the other
} infrared (940 nm). If the blood is oxygenated, it appears red because it
} absorbs red light, thus very little of the red beam passes through. If the
} blood has little oxygen, it appears blue & allows red light to pass. In
} both cases, the infrared beam will pass through. The amount of red vs
} infrared light detected on the other side of the finger is proportional to
} level of oxygen saturation.

Again I think you have this the wrong way round. Red Kool-Aid
looks red because it allows red light to pass through. Think of the
effect of shining a white torch beam through red KoolAid onto a
sheet of white paper. What colour is the beam? Red. Why,
because that is the predominant colour of light remaining in the
beam, blue and green having been removed by the KoolAid. A Blue
filter should attenuate a red laser more than a Red filter, assuming
the two are of the same colour strength.

The only way the red KoolAid can absorb more red light than the
blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
is present in the Blue KoolAid. This seems unlikely, but is not
impossible. Which flavour do you use for red, and which for blue?
The crimson effect of black cherry is almost certainly produced by
combining red and blue dyes, and there may be more blue dye in
black cherry than there is in ice-blue raspberry.

You might find the following web sites instructive

http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
y.html#dyestruct
http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
um.html
Chris

} I designed an experiment to show that a red laser pointer beam would be
} absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
} the laser beam darker if anything! PLEASE, can someone tell me what is
} going on? Is there any simple way to communicate this principle?? I
} appreciate your help.
}
} Mary
}
} ***************************************************
} Mary K.O'Connell
} Cardiovascular Biomechanics Research Lab
} MSLS: Room P224 - Surgery
} 1201 Welch Road
} Stanford, CA 94305
} (650) 723-1695
} (650) 498-6262 Fax
} E-mail: oconne1l-at-leland.stanford.edu
} (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} the first l, and an alphabetic "l" for the second.)
} *******************************************************
}

==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================

From daemon Thu May 2 09:13:29 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mary,

I think Chris is right. I got your message late last night and started
to reply that plants are green because they reflect green light, then I
in my sleepy state I started thinking that maybe something is different
about reflected versus transmitted light and I gave up trying to help.

However, this morning I still think the red should pass through red
Koolaid better than ultraviolet. I haven't had this stuff since my
undergrad about 20 years ago.

Karen Pawlowski, Ph.D.
UT Dallas

Chris Jeffree wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Mary
} Isn't this backwards? Oxygenated blood appears redder in
} transmitted or reflected light because it absorbs *blue*, and
} transmits or reflects the remaining red.
} } Basically, oxygen saturation is measured by
} } passing 2 light beams through the finger, one red light (660 nm), the other
} } infrared (940 nm). If the blood is oxygenated, it appears red because it
} } absorbs red light, thus very little of the red beam passes through. If the
} } blood has little oxygen, it appears blue & allows red light to pass. In
} } both cases, the infrared beam will pass through. The amount of red vs
} } infrared light detected on the other side of the finger is proportional to
} } level of oxygen saturation.
}
} Again I think you have this the wrong way round. Red Kool-Aid
} looks red because it allows red light to pass through. Think of the
} effect of shining a white torch beam through red KoolAid onto a
} sheet of white paper. What colour is the beam? Red. Why,
} because that is the predominant colour of light remaining in the
} beam, blue and green having been removed by the KoolAid. A Blue
} filter should attenuate a red laser more than a Red filter, assuming
} the two are of the same colour strength.
}
} The only way the red KoolAid can absorb more red light than the
} blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
} is present in the Blue KoolAid. This seems unlikely, but is not
} impossible. Which flavour do you use for red, and which for blue?
} The crimson effect of black cherry is almost certainly produced by
} combining red and blue dyes, and there may be more blue dye in
} black cherry than there is in ice-blue raspberry.
}
} You might find the following web sites instructive
}
} http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
} y.html#dyestruct
} http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
} um.html
} Chris
}
} } I designed an experiment to show that a red laser pointer beam would be
} } absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} } pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
} } the laser beam darker if anything! PLEASE, can someone tell me what is
} } going on? Is there any simple way to communicate this principle?? I
} } appreciate your help.
} }
} } Mary
} }
} } ***************************************************
} } Mary K.O'Connell
} } Cardiovascular Biomechanics Research Lab
} } MSLS: Room P224 - Surgery
} } 1201 Welch Road
} } Stanford, CA 94305
} } (650) 723-1695
} } (650) 498-6262 Fax
} } E-mail: oconne1l-at-leland.stanford.edu
} } (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} } the first l, and an alphabetic "l" for the second.)
} } *******************************************************
} }
}
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

From daemon Thu May 2 12:57:33 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ginger,

we do microtomy/TEM of bacterial samples with U and Tc in our lab. We take
special precautions at the block trimming part by collecting all the chips
on a piece of tape and disposing of them, as well as of the unused sections,
as a rad waste. I don't think my little routine of wiping the microtome
after finishing the work helps anything but my consciousness, however, it
may make you feel better, too. Since I am using a TEM that is NOT devoted to
the rad work (I think that will be also your case), I make a duplicate grid,
and run it through the scintillation counter. If the count doesn't exceed
background (most of the cases - imagine the dimensions of a section), I
consider it non rad. Your S35 beta should be a very safe bet.
Please feel free to contact me if you need any more info,
Alice.

Alice Dohnalkova
Environmental Microbiology
Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692 office
(509) 376-3654 TEM lab
fax (509) 376-1321

} From: Phoebe J Doss/app/Cvm [SMTP:pjdoss-at-cvm.okstate.edu]
Sent: Wednesday, May 01, 2002 2:23 PM
To: microscopy-at-sparc5.microscopy.com

"Ginger

Hendricks" To: "Doss, Phoebe and
Mike" {pjdoss-at-okstate.edu}
{grhendricks-at-cox. cc: (bcc: Phoebe J
Doss/app/Cvm)
net} Subject: please forward to
the MSA listserv

05/01/02 04:13 PM

Hello all,

I have a researcher who is interested in using autoradiography with nerve
tissue. His tech is researching methods and has come to me for advice. I
have never performed this procedure and am concerned about sectioning "hot"
(S35) tissue with the microtome. Any protocols, suggestions or direction
would be greatly appreciated.

My email address is grhendricks-at-cox.net

Thank you in advance,

Ginger

Ginger R. Hendricks
EM Lab Manager and Adjunct Instructor
OSU-CHS
1111 W. 17th St.
Tulsa, OK 74107
(918) 561-8232 work
(918) 699-8629 fax
grhendricks-at-cox.net
Website:
http://www.healthsciences.okstate.edu/research/rsp/electronmicroscopy/electr
on_microscopy_laboratory.htm

From daemon Thu May 2 13:08:27 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I suggest that you check out the following reference

Dickson, G.R., 1984; Chemical fixation and the preparation of calcified tissues for transmission electron microscopy
in: G.R. Dickson (ed) Methods of Calcified Tissue Research. New York, Elsevier, pp 79-148

Bob
Robert J. Schmitz
Department of Biology
University of Wisconsin Stevens Point
Stevens Point, WI 54481
715-346-2420
Email: rschmitz-at-uwsp.edu
http://biology.uwsp.edu/faculty/RSchmitz/home.html

} ----------
} From: Fernando Capela e Silva
} Sent: Wednesday, April 24, 2002 5:20 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: SEM and TEM for bone
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would like someone send me a general bone preparation scheme for SEM and
} TEM.
}
} With compliments
} * * * * * * * * * * * * * * * * * * * * * *
} Fernando Capela e Silva
} Laborat�rio de Biologia da Conserva��o
} Departamento de Biologia
} Universidade de �vora
} Apartado 94
} 7002-554 �vora
} PORTUGAL
}
} Phone: +351-266 760 800
} Fax: +351-266 711 231
} Email: fcs-at-uevora.pt
}
} http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm
}
}
}

From daemon Thu May 2 13:37:17 2002

Contents Retrieved from Microscopy Listserver Archives
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Thank you Michael. It is really nice of you!!!

Xianglin

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411

----- Original Message -----
} From: Michael Green {michael.green-at-UCL.AC.UK}

Hello all!!
I have a quick question for everyone.....We have a Hitachi
7500 TEM. We have been having a problem with "grainy" negatives, I
don't think the focus is as crisp as it should be. I have tried
smaller apertures (both obj. and cond. in various combinations). If
we take pictures of the same sample on a different microscope, the
micrographs are not grainy and the focus is much clearer (so it's not
the sample, the negatives, or the paper we're using....). I know the
optical density setting for the camera can be changed to increase
contrast, I have a feeling this would increase graininess as
well...is my hunch correct? The kicker is that we have had the same
optical density setting for weeks now and the graininess is just
getting worse (to my knowledge nothing has changed on the
microscope....). Would the use (or lack of use) of liquid Nitrogen
influence graininess? Any other ideas or comments?
Any help would be appreciated!!

Thanks,

Karli

From daemon Thu May 2 16:26:51 2002

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Check out a Beldon Cable catalog. They sell different gauges of a good grade of Copper cable (unshielded/unwrapped). The copper conductor in the cables is probably a good grade also, but it may not be OFHC (Oxygen-free High Conductivity). I have no idea whether Beldon has their catalog on the web or not.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

-----Original Message-----
} From: Vitaly Feingold [mailto:vitalylazar-at-worldnet.att.net]
Sent: Thursday, May 02, 2002 2:47 AM
To: Microscopy-at-sparc5.microscopy.com; Earl Weltmer (by way of
MicroscopyListserver)

Earl, try these:

1) Malin Co., 5400 Smith Road, Brook Park, OH 44142. Specialty wire. Tel.
(216)267-9080. I don't know if they have a web site.
2) Alfa Aesar, www.alfa.com , (800)343-0660, and (800)343-7276 for tech
support.
3) Little Falls Alloys, www.lfa-wire.com , (888)5329473 or (973)278-1666.

Alfa will probably have the purest Copper, as far as Oxygen and other
impurities go (the best is 99,9999% metals basis). Little Falls Alloys may
have more suitable specialty Copper alloy, especially for high temperature
apps.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Earl Weltmer (by way of MicroscopyListserver) {earlw-at-sbcglobal.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 01, 2002 7:36 PM

Heaven forbid that I should comment on something in the life science, so I won't. I don't think that Chris is correct in his explanation.

Let me site the example of a thin film of gold. If it is thin enough, when you look at the gold in transmission, it is green. In reflection it is gold because the gold is absorbing the green and allowing all other wavelengths of light to be reflected. If you make the gold thicker the intensity of the green getting through decreases exponentially with thickness until eventually it is opaque. A series of thickness of gold films from very thin to very thick illustrates the point very well.

I suspect that the absorption mechanism with the oxygen is related to the iron in the blood. Isn't it iron in the blood that carries the oxygen? With more oxygenated iron, a higher absorption occurs, i.e. the red light gets absorbed more. This is analogous to your beam getting darker. I would say that to show a less oxygenated example, you have to dilute your Cool-Aid.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

-----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
Sent: Thursday, May 02, 2002 6:45 AM
To: Mary K. O'Connell
Cc: microscopy-at-sparc5.microscopy.com

Mary
Isn't this backwards? Oxygenated blood appears redder in
transmitted or reflected light because it absorbs *blue*, and
transmits or reflects the remaining red.
} Basically, oxygen saturation is measured by
} passing 2 light beams through the finger, one red light (660 nm), the other
} infrared (940 nm). If the blood is oxygenated, it appears red because it
} absorbs red light, thus very little of the red beam passes through. If the
} blood has little oxygen, it appears blue & allows red light to pass. In
} both cases, the infrared beam will pass through. The amount of red vs
} infrared light detected on the other side of the finger is proportional to
} level of oxygen saturation.

Again I think you have this the wrong way round. Red Kool-Aid
looks red because it allows red light to pass through. Think of the
effect of shining a white torch beam through red KoolAid onto a
sheet of white paper. What colour is the beam? Red. Why,
because that is the predominant colour of light remaining in the
beam, blue and green having been removed by the KoolAid. A Blue
filter should attenuate a red laser more than a Red filter, assuming
the two are of the same colour strength.

The only way the red KoolAid can absorb more red light than the
blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
is present in the Blue KoolAid. This seems unlikely, but is not
impossible. Which flavour do you use for red, and which for blue?
The crimson effect of black cherry is almost certainly produced by
combining red and blue dyes, and there may be more blue dye in
black cherry than there is in ice-blue raspberry.

You might find the following web sites instructive

http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
y.html#dyestruct
http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
um.html
Chris

} I designed an experiment to show that a red laser pointer beam would be
} absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
} the laser beam darker if anything! PLEASE, can someone tell me what is
} going on? Is there any simple way to communicate this principle?? I
} appreciate your help.
}
} Mary
}
} ***************************************************
} Mary K.O'Connell
} Cardiovascular Biomechanics Research Lab
} MSLS: Room P224 - Surgery
} 1201 Welch Road
} Stanford, CA 94305
} (650) 723-1695
} (650) 498-6262 Fax
} E-mail: oconne1l-at-leland.stanford.edu
} (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} the first l, and an alphabetic "l" for the second.)
} *******************************************************
}

==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================

From daemon Thu May 2 17:12:47 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yeeess!, but haemoglobin is not reflective like gold. The gold
phenomenon is caused by strong forward reflection of red/yellow light,
and transmission of remaining green. Blood cells have no surface
reflectivity to speak of. Blood is more like an emulsion of a red
transparent phase in a pale yellow transparent phase. The oxygenated
rbcs absorb blue, transmitting red, but they also cause light
scattering, and some red light is forward scattered as a result.
Unlike a film of gold, blood is red in reflected and transmitted
light.

Now how do you explain the fact that colloidal gold appears red
against the light i.e. red is transmitted??
Chris

p.s. you're allowed - we're talking physics here
} Heaven forbid that I should comment on something in the life
science, so I won't. I don't think that Chris is correct in his
explanation.
}
} Let me site the example of a thin film of gold. If it is thin
enough, when you look at the gold in transmission, it is green. In
reflection it is gold because the gold is absorbing the green and
allowing all other wavelengths of light to be reflected. If you make
the gold thicker the intensity of the green getting through decreases
exponentially with thickness until eventually it is opaque. A series
of thickness of gold films from very thin to very thick illustrates
the point very well.
}
} I suspect that the absorption mechanism with the oxygen is related
to the iron in the blood. Isn't it iron in the blood that carries the
oxygen? With more oxygenated iron, a higher absorption occurs, i.e.
the red light gets absorbed more. This is analogous to your beam
getting darker. I would say that to show a less oxygenated example,
you have to dilute your Cool-Aid.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} P. O. Box 11472 (letters)
} Guys Run Rd. (packages)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8515 (fax)
}
}
}
} -----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
} Sent: Thursday, May 02, 2002 6:45 AM
} To: Mary K. O'Connell
} Cc: microscopy-at-sparc5.microscopy.com
} Subject: Re: Basic Science - oxygen saturation example
}
}
} --------------------------------------------------------------------
----
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
---.
}
}
} Mary
} Isn't this backwards? Oxygenated blood appears redder in
} transmitted or reflected light because it absorbs *blue*, and
} transmits or reflects the remaining red.
} } Basically, oxygen saturation is measured by
} } passing 2 light beams through the finger, one red light (660 nm),
the other
} } infrared (940 nm). If the blood is oxygenated, it appears red
because it
} } absorbs red light, thus very little of the red beam passes
through. If the
} } blood has little oxygen, it appears blue & allows red light to
pass. In
} } both cases, the infrared beam will pass through. The amount of red
vs
} } infrared light detected on the other side of the finger is
proportional to
} } level of oxygen saturation.
}
} Again I think you have this the wrong way round. Red Kool-Aid
} looks red because it allows red light to pass through. Think of the
} effect of shining a white torch beam through red KoolAid onto a
} sheet of white paper. What colour is the beam? Red. Why,
} because that is the predominant colour of light remaining in the
} beam, blue and green having been removed by the KoolAid. A Blue
} filter should attenuate a red laser more than a Red filter, assuming
} the two are of the same colour strength.
}
} The only way the red KoolAid can absorb more red light than the
} blue is if it contains more red-absorbing (i.e. cyan or blue) dye
than
} is present in the Blue KoolAid. This seems unlikely, but is not
} impossible. Which flavour do you use for red, and which for blue?
} The crimson effect of black cherry is almost certainly produced by
} combining red and blue dyes, and there may be more blue dye in
} black cherry than there is in ice-blue raspberry.
}
} You might find the following web sites instructive
}
} http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
} y.html#dyestruct
} http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
} um.html
} Chris
}
} } I designed an experiment to show that a red laser pointer beam
would be
} } absorbed by red Kool-Aid, while blue Kool-Aid would allow the red
beam to
} } pass through. Unfortunately, this is not the case!!! The red
Kool-Aid makes
} } the laser beam darker if anything! PLEASE, can someone tell me
what is
} } going on? Is there any simple way to communicate this principle??
I
} } appreciate your help.
} }
} } Mary
} }
} } ***************************************************
} } Mary K.O'Connell
} } Cardiovascular Biomechanics Research Lab
} } MSLS: Room P224 - Surgery
} } 1201 Welch Road
} } Stanford, CA 94305
} } (650) 723-1695
} } (650) 498-6262 Fax
} } E-mail: oconne1l-at-leland.stanford.edu
} } (Note: O'Connell in the E-mail address is spelled with a numeric
"1" for
} } the first l, and an alphabetic "l" for the second.)
} } *******************************************************
} }
}
}
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

From daemon Thu May 2 17:27:37 2002

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I don't want to make this more complicated as it is, but do you know, how
Koolaid gets its color?

Could be straight exitation and relaxation. Then Koolaid would absorb a
photon and emit it again. Since the emission should be into 4 Pi solid
angle, the effect would be, that fewer photons of that color reach the eye,
i.e., this color would be absorbed.

Could be a "fluorescence" effect, where Koolaid absorbs a high energy photon
(blue), then emits a red photon and an IR photon. In this case the red parts
of the spectrum would be enhanced.

Could be scattering, as in "why is the sky blue".

And then, the laser frequency may not be the one that is actually absorbed
by Koolaid. Red is not necesarily red.

I think, without knowing exactly what it is that makes Koolaid red it is
hard to predict what happens. And if that then can be applied to the color
of blood is another step.

It might be easier to start with something different than Koolaid.

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: Karen Pawlowski [mailto:kpawlow-at-swbell.net]
Sent: Thursday, May 02, 2002 7:59 AM
To: MSA listserver submission

Mary,

I think Chris is right. I got your message late last night and started
to reply that plants are green because they reflect green light, then I
in my sleepy state I started thinking that maybe something is different
about reflected versus transmitted light and I gave up trying to help.

However, this morning I still think the red should pass through red
Koolaid better than ultraviolet. I haven't had this stuff since my
undergrad about 20 years ago.

Karen Pawlowski, Ph.D.
UT Dallas

Chris Jeffree wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} Mary
} Isn't this backwards? Oxygenated blood appears redder in
} transmitted or reflected light because it absorbs *blue*, and
} transmits or reflects the remaining red.
} } Basically, oxygen saturation is measured by
} } passing 2 light beams through the finger, one red light (660 nm), the
other
} } infrared (940 nm). If the blood is oxygenated, it appears red because it
} } absorbs red light, thus very little of the red beam passes through. If
the
} } blood has little oxygen, it appears blue & allows red light to pass. In
} } both cases, the infrared beam will pass through. The amount of red vs
} } infrared light detected on the other side of the finger is proportional
to
} } level of oxygen saturation.
}
} Again I think you have this the wrong way round. Red Kool-Aid
} looks red because it allows red light to pass through. Think of the
} effect of shining a white torch beam through red KoolAid onto a
} sheet of white paper. What colour is the beam? Red. Why,
} because that is the predominant colour of light remaining in the
} beam, blue and green having been removed by the KoolAid. A Blue
} filter should attenuate a red laser more than a Red filter, assuming
} the two are of the same colour strength.
}
} The only way the red KoolAid can absorb more red light than the
} blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
} is present in the Blue KoolAid. This seems unlikely, but is not
} impossible. Which flavour do you use for red, and which for blue?
} The crimson effect of black cherry is almost certainly produced by
} combining red and blue dyes, and there may be more blue dye in
} black cherry than there is in ice-blue raspberry.
}
} You might find the following web sites instructive
}
} http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
} y.html#dyestruct
} http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
} um.html
} Chris
}
} } I designed an experiment to show that a red laser pointer beam would be
} } absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam
to
} } pass through. Unfortunately, this is not the case!!! The red Kool-Aid
makes
} } the laser beam darker if anything! PLEASE, can someone tell me what is
} } going on? Is there any simple way to communicate this principle?? I
} } appreciate your help.
} }
} } Mary
} }
} } ***************************************************
} } Mary K.O'Connell
} } Cardiovascular Biomechanics Research Lab
} } MSLS: Room P224 - Surgery
} } 1201 Welch Road
} } Stanford, CA 94305
} } (650) 723-1695
} } (650) 498-6262 Fax
} } E-mail: oconne1l-at-leland.stanford.edu
} } (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} } the first l, and an alphabetic "l" for the second.)
} } *******************************************************
} }
}
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Waddington Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JN, Scotland, UK
} Tel. #44 (0) 131 650 5554
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

From daemon Thu May 2 17:57:59 2002

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Karli

If it's focus problem, try to make 'focus series' - the same area with
1,2,3 clicks to the left and to the right. You have to see the changes on
the negs (and on the screen). I usually align microscope in the way that I
am able to see changes from underfocus to overfocus in 3 clicks (it's
JEM1200EX, coarse control) at x60-80K. So, 1st click is underfocus, next-
focus, next - overfocus. If you do not see it, you have, probably, problem
with alignment. I don't know how this related to your particular
problem. In biological applications we are usually using amplitude
contrast, so it's close to the actual focus. At this point you have
minimal visual contrast. From that you defocus (to increase contrast and
'granularity' will go up too) your image. The degree of defocus depends
what you want to see.

Personally, I would suspect film/developer/technician. So many things: bad
batch of the film, film stored at the high temp, contaminated developer,
unproper dilution etc. Try to make blank image (no sample in the
microscope), turn off automatic exposure and make a few shots with
different exposure time (around automatic measurements, say automatic is 2
sec, make 1, 1.5, 2, 3 sec) and develop altogether....

Sergey

At 12:56 PM 5/2/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Thu May 2 19:12:56 2002

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We have stained annelid embryos with FITC-conjugated anti-tubulin antibody
and propidium iodide. We are using a Biorad-MRC-600 and seem to be getting
almost identical signals(of nuclei and a few mitochondria) on both the 488
and 568 wavelengths, even though when viewed with epifluorescence, the
tubulin stain seems to distinctly define cell borders. I understood that
the propidium iodide emission would not be overlapping with that of
FITC--is this incorrect? I have other double-labelled samples which are
stained with different stains, but their signals are within the
appropriate emission spectra for both confocal and epifluorescence, so the
detection filters on the confocal do not seem to be the problem.

Any feedback is appreciated!

Pauline Yu
pcy-at-usc.edu
Manahan Lab
http://www.usc.edu/manahanlab

From daemon Thu May 2 19:54:02 2002

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I was considering purchasing a Kodak MDS 100 Digital camera to permanently
attach to one of my microscopes for examining specimens directly on my
computer CRT. Unfortunately as far as I can determine the software supplied
only supports Win 95 and 98. I'd like to know if anyone is currently using a
MDS 100 on ME or XP and how (or if) it works.
Thanks in advance
P Polsgrove

Pete Polsgrove
NAU Flagstaff, AZ.
pjp6-at-dana.ucc.nau.edu
micro2001p-at-netscape.net

From daemon Thu May 2 20:37:22 2002

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Karli, do grainy negatives look denser? If so, they may be overexposed (TEM
setting/exp.meter problem), or overdeveloped
(developer/temperature/time/stop bath). I assume here that the batch of film
is fine. If grainy negatives don't look denser, then the problem is likely
with TEM. It's difficult to say whether you are dealing with real photo
emulsion grain, or rather with sample/TEM artifact, without seeing the
negative.

Speaking of the emulsion grain- it will also appear on the normal density
negatives, in the following cases: film improperly stored or expired,
negatives were overexposed and underdeveloped or vice versa, in order to
keep density normal. Then, bad developer (contaminated/exhausted), or bad
film.

Use Sergey's advice and take a few blanks. It's hard to imagine a TEM
problem causing that, other than incorrect exposure meter reading.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Karli Fitzelle (by way of MicroscopyListserver) {fitzelle.1-at-osu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 02, 2002 3:56 PM

Mary,
As someone who has used a pulse oximeter before a double lung transplant, I
decided to dig out my manual. I am copying the following FYI:

"The pulse oximeter shines red and infrared light through the tissue and
detects the fluctuating signals caused by arterial blood pulses. The ratio
of the fluctuations of the red and infrared light signals received
determines the oxygen saturation content. Conditions such as steady venous
blood flow, skin thickness, finger nail thickness, etc. do not effect the
saturation reading because they are constant and do not cause fluctuations."

(the function is then given as a formula; it goes on saying)

"Note that the oximeter's readings do not depend upon the absolute light
intensity, rather upon the fluctuations in light intensity."

It goes on with other comments about skin pigmentation, if there is too
little light, etc.

"Pulse oximeters use two different wavelengths of light (red and infrared),
providing the ability to determine one component of the blood. The oximeter
is calibrated to closely approximate functional oxygen saturation values.
These values will closely approximate laboratory instrument functional
saturation values if the dysfunctional hemoglobin saturation levels are
negligible. If the dysfunctional hemoglobin is carboxyhemoglobin or
methemoglobin, the difference between the oxygen saturation value displayed
by the oximeter and the oxygen saturation values determined by the
laboratory instrument will be greater as the dysfunctional hemoglobin levels
rise approximately in accordance with the following formula: " (it then
gives the formula).

I wonder if the issue is not "red blood" but how the red and infrared light
interact with oxygenated and deoxygenated hemoglobin and not what we see as
arterial (red) blood or venous (blue) blood. I don't think that this is an
easy principle for 4th graders.

Chris gives a correct explanation about light absorption and the dyes used
in Kool-Aid.

PS I hope this is part of a "Don't smoke" program!!!

Damian

Damian Neuberger, PhD
Senior Research Scientist
Baxter Healthcare Corp
P.O. Box 490
Round Lake, IL 60073
Tel: 847.270.5888
Fax: 847.270.5897
damian_neuberger-at-baxter.com

} Need Your Help!!
}
} I am trying to teach 4th graders how an oxygen saturation meter works, &
my
} experiment is FAILING!! Basically, oxygen saturation is measured by
} passing 2 light beams through the finger, one red light (660 nm), the
other
} infrared (940 nm). If the blood is oxygenated, it appears red because it
} absorbs red light, thus very little of the red beam passes through. If the
} blood has little oxygen, it appears blue & allows red light to pass. In
} both cases, the infrared beam will pass through. The amount of red vs
} infrared light detected on the other side of the finger is proportional to
} level of oxygen saturation.
}
} I designed an experiment to show that a red laser pointer beam would be
} absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} pass through. Unfortunately, this is not the case!!! The red Kool-Aid
makes
} the laser beam darker if anything! PLEASE, can someone tell me what is
} going on? Is there any simple way to communicate this principle?? I
} appreciate your help.
}
} Mary
}
} ***************************************************
} Mary K.O'Connell
} Cardiovascular Biomechanics Research Lab
} MSLS: Room P224 - Surgery
} 1201 Welch Road
} Stanford, CA 94305
} (650) 723-1695
} (650) 498-6262 Fax
} E-mail: oconne1l-at-leland.stanford.edu
} (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} the first l, and an alphabetic "l" for the second.)
} *******************************************************
}
}

From daemon Thu May 2 21:54:49 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Peter,

Check with Calumet Photographic. Their web address is

www.calumetphoto.com

Damian Neuberger

} Folks;
}
} Does anyone know of a mfg. that makes a camera, for general photography,
} that will accomodate 4x5" film like Polaroid Type 57, that uses a Graflok
} back? I checked with Polaroid but they could offer no assistance.
}
} Thanks,
}
} Peter Tomic
} Anadigics, Inc.

From daemon Thu May 2 23:50:31 2002

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Scott and Chris

Talking about ultra-fine gold foil or gold colloidal suspension, we have to
keep in mind the'wave-nature' of the light. When thickness of material is
comparable to wavelength, so may magical things is happening: suspended
tiny gold particles colored solution in the red. Moreover, the color
depends from the particle size - you could make it brownish or greenish -
depends how much tannic acid you add to make collodial gold. Over
moreover: you could calculate the particles size from the known
spectrum. Similar things is happening with fine gold foil. I was a bad
student in the physic class. I do remember it's waves. If particle is
smaller than 1/2 wavelength, the wave will not interfere with it. Red
light has longer wavelength and pass gold particles when other has
adsorbed. I hope it's how they teach us... Sorry if I am wrong. Similar
to lasers. It's monochromatic and coherent. To filter it you should use
appropriate things: diffraction (pattern?) filters (they work similarly:
let pass wavelength longer then the size of the strips). If KoolAid is
looks red it does not mean it will be transparent to the laser
wavelength. I think the set of simple photographic filters (red/blue;
yellow/green) may do better job (I mean, no lasers).

Sergey

At 02:43 PM 5/2/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Fri May 3 01:15:42 2002

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From daemon Fri May 3 03:25:09 2002

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{bold} {color} {param} 0100,0100,0100 {/param} To the Microscopy & Microanalysi=
s community

{/bold} Ref.: Anonymous Message posted April 30,

Subj: R=D6NTEC USA Closes Down Operation

Ladies and Gentlemen:

Without any doubt, the Microscopy ListServer is a great facility for
the Microscopy&Microanalysis community to distribute and
exchange useful information in a very efficient way and everyone
involved in initiation and maintenance of this forum highly deserves
our community=92s credit.

However, it appears that at the same time this platform is a quite
powerful tool for anyone with hostile and bad enough intents to
defame and defile disliked entities. This is what we had to learn
when the unwarranted message regarding an alleged close down of
R=D6NTEC USA, Inc. was spread. It also appears that there is no
way to avoid such unfounded, false and misleading information to
be posted at the ListServer other than the commitment of all users
to the =93General Ground Rules on using the Microscopy
Listserver/Mailreflector=94 (see msa website), clearly a code of honor.
Whatever individual or organization posted this message in their
malicious attempt of damaging our company and good reputation
violated all those general ground rules. May everyone draw his/her
own conclusions concerning the nature of the information and the
integrity of the sender.

We may be allowed to correct the above-mentioned statements
about R=D6NTEC USA, Inc. as follows:

{paraindent} {param} out {/param} 1. There are no plans whatsoever to cease th=
e business of
R=D6NTEC USA, Inc. {/paraindent}

{paraindent} {param} out {/param} 2. The R=D6NTEC Group of companies =96 like =
so many others =96
has not remained unaffected by the overall downturn we
saw in the economy during the year 2001. The situation
within our group forced us to implement a restructuring
program in order to reduce operational costs (as was the
case throughout the industry =96 we believe we are in good
company here). This program was comprising all members
of our group, including RONTEC USA. The goal was not to
cease business, but exactly the opposite - to ensure its
continuation, in the best interest of our customers. {/paraindent}

{paraindent} {param} out {/param} 3. Our joint efforts have been successful. T=
he fact is
R=D6NTEC USA, Inc. has had its best sales year since the
company=92s founding in 1999 nearly doubling its revenue
over the previous year. Why, after having worked very hard
indeed to get things going and after having eventually
accomplished that stage, should we consider to
discontinue the business? Apparently there exists
someone or some company who is attempting to damage
our reputation. Be assured that R=D6NTEC USA is in a
better position than ever before and will continue to grow
and continue to support its customers. {/paraindent}

{paraindent} {param} out {/param} 4. We have never provided misleading informa=
tion on the
equipment we are offering. How long would we survive in
this business if we were to make promises we can=92t keep?
Due to our companies policy of verifying any information
before disclosing it to the public we earned ourselves a
reputation as a reliable business partner. {/paraindent}

{paraindent} {param} out {/param} 5. R=D6NTEC USA, Inc. does have an SEM for s=
ale, however
this has absolutely no bearing on the viability of the
company. {/paraindent}

Whoever has any questions or the need for more information on
this subject may please contact us directly, by phone or otherwise,
in the US or at our German headquarters. We at R=D6NTEC take
great pride in our products and services and we have nothing to
hide =96 in contrast to the sender of that unfounded message, who
chose not to disclose a name.

In the future may our community be spared such unwarranted
assaults.

Thomas Schuelein Paul Smith

President & CEO President & CEO

R=D6NTEC Holding AG R=D6NTEC USA, Inc.

{nofill}

From daemon Fri May 3 05:59:29 2002

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Hi folks - I'm familar with prep for TEM, SEM, etc., but not confocal -
would you send me a good protocol for this type of scope for tissue or
single cells?
Thanks
Barb

From daemon Fri May 3 05:59:34 2002

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Hi folks - I'm familar with prep for TEM, SEM, flow, etc., but not this
type of scope, would someone send me a good protocol for tissue and
single cells?
Thanks
Barb

From daemon Fri May 3 06:01:54 2002

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Yes, they do reflect green light. But they also transmit green light.
Chlorophylls a and b have two strong absorption peaks very roughly
between 400&500nm and between 650 and 700nm. The remaining
central part of the visible spectrum appears green, but also
contains cyan, yellow and orange.

} I think Chris is right. I got your message late last night and
started
} to reply that plants are green because they reflect green light, then I
} in my sleepy state I started thinking that maybe something is different
} about reflected versus transmitted light and I gave up trying to help.
}
} However, this morning I still think the red should pass through red
} Koolaid better than ultraviolet. I haven't had this stuff since my
} undergrad about 20 years ago.
}
} Karen Pawlowski, Ph.D.
} UT Dallas
}
} Chris Jeffree wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Mary
} } Isn't this backwards? Oxygenated blood appears redder in
} } transmitted or reflected light because it absorbs *blue*, and
} } transmits or reflects the remaining red.
} } } Basically, oxygen saturation is measured by
} } } passing 2 light beams through the finger, one red light (660 nm), the other
} } } infrared (940 nm). If the blood is oxygenated, it appears red because it
} } } absorbs red light, thus very little of the red beam passes through. If the
} } } blood has little oxygen, it appears blue & allows red light to pass. In
} } } both cases, the infrared beam will pass through. The amount of red vs
} } } infrared light detected on the other side of the finger is proportional to
} } } level of oxygen saturation.
} }
} } Again I think you have this the wrong way round. Red Kool-Aid
} } looks red because it allows red light to pass through. Think of the
} } effect of shining a white torch beam through red KoolAid onto a
} } sheet of white paper. What colour is the beam? Red. Why,
} } because that is the predominant colour of light remaining in the
} } beam, blue and green having been removed by the KoolAid. A Blue
} } filter should attenuate a red laser more than a Red filter, assuming
} } the two are of the same colour strength.
} }
} } The only way the red KoolAid can absorb more red light than the
} } blue is if it contains more red-absorbing (i.e. cyan or blue) dye than
} } is present in the Blue KoolAid. This seems unlikely, but is not
} } impossible. Which flavour do you use for red, and which for blue?
} } The crimson effect of black cherry is almost certainly produced by
} } combining red and blue dyes, and there may be more blue dye in
} } black cherry than there is in ice-blue raspberry.
} }
} } You might find the following web sites instructive
} }
} } http://www.dartmouth.edu/~chemlab/chem6/dyes/full_text/chemistr
} } y.html#dyestruct
} } http://www.dartmouth.edu/~chemlab/info/resources/spectrum/spectr
} } um.html
} } Chris
} }
} } } I designed an experiment to show that a red laser pointer beam would be
} } } absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} } } pass through. Unfortunately, this is not the case!!! The red Kool-Aid makes
} } } the laser beam darker if anything! PLEASE, can someone tell me what is
} } } going on? Is there any simple way to communicate this principle?? I
} } } appreciate your help.
} } }
} } } Mary
} } }
} } } ***************************************************
} } } Mary K.O'Connell
} } } Cardiovascular Biomechanics Research Lab
} } } MSLS: Room P224 - Surgery
} } } 1201 Welch Road
} } } Stanford, CA 94305
} } } (650) 723-1695
} } } (650) 498-6262 Fax
} } } E-mail: oconne1l-at-leland.stanford.edu
} } } (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} } } the first l, and an alphabetic "l" for the second.)
} } } *******************************************************
} } }
} }
} } ==========================================
} } Dr. Chris Jeffree
} } University of Edinburgh
} } BIOSEM - Biological Sciences Electron Microscope Facility
} } Institute of Cell and Molecular Biology
} } Waddington Building
} } King's Buildings, Mayfield Road
} } EDINBURGH, EH9 3JN, Scotland, UK
} } Tel. #44 (0) 131 650 5554
} } FAX. #44 (0) 131 650 6563
} } Mobile 07710 585 401
} } email c.jeffree-at-ed.ac.uk
} } =========================================
}
}

==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================

From daemon Fri May 3 07:12:57 2002

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} From a processing point of view:

Every time I have had a really excessive grain problem it was from using too
strong a concentration of developer, way too strong or I was underexposing
the film and over developing it. The first was usualy by accident and only
caused a problem when I was working with a completely unknown set of
conditions such as infra red photography, a new film and a mistake in
dilution where I had to establish exposure time, development time without
reliable tools to measure the intensity of the infra red light or working
with some new combination of film and some unconventional developer. I the
last case it was usualy intentionally trying to take pictures in light to
low for the film I had.

Gordon

} From: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
: Karli
:
: If it's focus problem, try to make 'focus series' - the same area with
: 1,2,3 clicks to the left and to the right. You have to see the changes on
: the negs (and on the screen). I usually align microscope in the way that I
: am able to see changes from underfocus to overfocus in 3 clicks (it's
: JEM1200EX, coarse control) at x60-80K. So, 1st click is underfocus, next-
: focus, next - overfocus. If you do not see it, you have, probably,
problem
: with alignment. I don't know how this related to your particular
: problem. In biological applications we are usually using amplitude
: contrast, so it's close to the actual focus. At this point you have
: minimal visual contrast. From that you defocus (to increase contrast and
: 'granularity' will go up too) your image. The degree of defocus depends
: what you want to see.
:
: Personally, I would suspect film/developer/technician. So many things:
bad
: batch of the film, film stored at the high temp, contaminated developer,
: unproper dilution etc. Try to make blank image (no sample in the
: microscope), turn off automatic exposure and make a few shots with
: different exposure time (around automatic measurements, say automatic is 2
: sec, make 1, 1.5, 2, 3 sec) and develop altogether....
:
: Sergey
:
: At 12:56 PM 5/2/02, you wrote:
: } ------------------------------------------------------------------------
: } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
: } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
: } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
: } -----------------------------------------------------------------------.
: }
: }
: } Hello all!!
: } I have a quick question for everyone.....We have a Hitachi 7500
: } TEM. We have been having a problem with "grainy" negatives, I don't
think
: } the focus is as crisp as it should be. I have tried smaller apertures
: } (both obj. and cond. in various combinations). If we take pictures of
the
: } same sample on a different microscope, the micrographs are not grainy
and
: } the focus is much clearer (so it's not the sample, the negatives, or the
: } paper we're using....). I know the optical density setting for the
camera
: } can be changed to increase contrast, I have a feeling this would
increase
: } graininess as well...is my hunch correct? The kicker is that we have had
: } the same optical density setting for weeks now and the graininess is
just
: } getting worse (to my knowledge nothing has changed on the
: } microscope....). Would the use (or lack of use) of liquid Nitrogen
: } influence graininess? Any other ideas or comments?
: } Any help would be appreciated!!
: }
: } Thanks,
: }
: } Karli
:
: _____________________________________
:
: Sergey Ryazantsev Ph. D.
: Electron Microscopy
: UCLA School of Medicine
: Department of Biological Chemistry
: Box 951737
: Los Angeles, CA 90095-1737
:
: Phone: (310) 825-1144
: FAX (departmental): (310) 206-5272
: mailto:sryazant-at-ucla.edu
:
:
:
:
:

From daemon Fri May 3 07:46:50 2002

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It's strange to see that one anonymous message could make so much damage to
the company. It seems to me that reputation of the company depends more on
the quality of the products/service etc nor on the one anonymous
opinion. From another hand, I think, this ListServer mostly is a place for
'customers' to exchange not only scientific ideas but to help each other to
survive in this 'capitalistic' world. Information about wrongdoing may
help others do not make similar mistake or spend money on bad quality
product/service. This is sort of our 'protection' against bad quality
service/products providers. Again, single decision even published here
could not damage the reputation of the good company with long well
established record of the service to EM community. I know, there are
places on the Internet where you could leave your opinion about some
particular business/company - it's perfectly legal and many institutions
used that lists for decision making. This discussion is more about ethics
- we all agree that we have rights openly speak here and others have the
rights to know who is speaking.

Sergey spoke.

At 02:15 AM 5/3/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Fri May 3 09:40:31 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Morning All,

To get a better sense of this thread of conversation I went looking for a
source of concentration on this specific subject. This continued an attempt
some time ago when the question came up on another 'List'.

As we learn from one another, sometimes it helps to find a source of
expertise who appears to have actually been involved in the engineering of
the type of device in question - or something like it. Further, the
following URL demonstrates that this conversation is ALL about physics

Thus, I recommend some outside reading at:
http://lfd.uiuc.edu/staff/maier/thesis/index.html

Is it widely known that some arctic fish travel contentedly sans RBCs? For
more information look in old 'Comparative Biochemistry' by Baldwin. Short
book from Methuen and a longer one from Cambridge. Also a reading from Sci.
Am.: http://oak.cats.ohiou.edu/~piccard/scientam/globins.html

Also, HIGHLY recommended is the translation of Zinoffsky's original paper on
hemoglobin: http://www.udel.edu/chem/white/teaching/CHEM342/ZinBkgd99.html

For me life is just a constant stream of history and future, mixed in the
present. As George Patton said (about something else!), "God help me, I do
love it so."

Regards,

Fred Monson

P.S. Award for best Commencement Address ever goes to Kurt Vonnegut (1997 -at-
MIT) even if he didn't give it:
http://www.tiac.net/users/sqltech/document/vonegut.txt

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

} ----------
} From: Mary K. O'Connell
} Sent: Wednesday, May 1, 2002 4:45 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Basic Science - oxygen saturation example
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Need Your Help!!
}
} I am trying to teach 4th graders how an oxygen saturation meter works, &
} my
} experiment is FAILING!! Basically, oxygen saturation is measured by
} passing 2 light beams through the finger, one red light (660 nm), the
} other
} infrared (940 nm). If the blood is oxygenated, it appears red because it
} absorbs red light, thus very little of the red beam passes through. If the
}
} blood has little oxygen, it appears blue & allows red light to pass. In
} both cases, the infrared beam will pass through. The amount of red vs
} infrared light detected on the other side of the finger is proportional to
}
} level of oxygen saturation.
}
} I designed an experiment to show that a red laser pointer beam would be
} absorbed by red Kool-Aid, while blue Kool-Aid would allow the red beam to
} pass through. Unfortunately, this is not the case!!! The red Kool-Aid
} makes
} the laser beam darker if anything! PLEASE, can someone tell me what is
} going on? Is there any simple way to communicate this principle?? I
} appreciate your help.
}
} Mary
}
} ***************************************************
} Mary K.O'Connell
} Cardiovascular Biomechanics Research Lab
} MSLS: Room P224 - Surgery
} 1201 Welch Road
} Stanford, CA 94305
} (650) 723-1695
} (650) 498-6262 Fax
} E-mail: oconne1l-at-leland.stanford.edu
} (Note: O'Connell in the E-mail address is spelled with a numeric "1" for
} the first l, and an alphabetic "l" for the second.)
} *******************************************************
}
}

From daemon Fri May 3 09:40:32 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try food coloring.

From daemon Fri May 3 09:41:21 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

At 09:37 PM 5/2/2002 -0500, Damian Neuberger wrote:
} I wonder if the issue is not "red blood" but how the red and infrared light
} interact with oxygenated and deoxygenated hemoglobin and not what we see as
} arterial (red) blood or venous (blue) blood. I don't think that this is an
} easy principle for 4th graders.

Yes, half of them will come away thinking that red Kool-Aid has
blood in it. :-) The good news is that 20% of them weren't
paying attention and won't retain this "fact".

- John

From daemon Fri May 3 11:28:16 2002

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Hi everyone,

I need to do flat embedding using LR White. A Teflon mold covered with ACLAR seems to be the answer. I am afraid that the over filled resin under ACLAR may not polimerize properly and become messy. I appreciate your experience.

Ann Fook Yang

From daemon Fri May 3 12:00:48 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All:
I am trying to convert an image and spectra from the Oxford AN 10000 EDS
to
a .tiff that is readable on a PC. I've done it in the past, but it has
been so long, I forget the exact
procedure. I hope that there is somone out there that does this more
regularly that can refresh my memory.
Thanks in advance,
Mike Coviello
UT Arlington

From daemon Fri May 3 12:08:13 2002

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Dear Microscopists,

The following TEM, which is capable of producing images with 0.17
nm resolution, but has gun/filament instability problems especially above
300 KV, is available to anyone willing to take responsibility for removing
it from its present location prior to August 2002. There is no charge
other than the costs involved with dismantling, moving, shipping and
reinstallation. It is a top-entry version of a JEOL 4000. The only
accessory is a Gatan image intensifier and TV camera. It is estimated that
it would take two weeks to dismantle and pack properly for reuse. It is
located in Ithaca, NY at Cornell University's Center for Materials Research.

John Hunt
CCMR Microscopy Facility
(607) 255-0108

From daemon Fri May 3 13:07:45 2002

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} Try food coloring.
Tried it. Didn't like it. Fact is green potatoes and bright purple
steak
just doesn't get eaten.

The take-home from this discussion, as pointed out by several
respondents, is that there are several ways to arrive at "red" in
Kool-Aid, inkjet inks, food colouring, whatever. For example, a red
which exactly matches the apparent colour of a laser pointer could be
produced by a single red dye, or, as in an inkjet printer, from a
blend of yellow and magenta dyes. A deeper cherry red from your
printer will be a blend of yellow, magenta and cyan dyes. What is
required is some data on the absorption/transmission characteristics
of different reds and blues. The best thing would be to run an
absorption spectrum using a spectrophotometer. If there is no chance
of access to one then it might be instructive to do some simple
chromatography. Place drops of concentrated solutions of fibre-tip
pen, fabric dye, food colouring or Kool-Aid one inch from the long
edge of a ~12" x 6" piece of filter paper or blotting paper. Curl
the paper into a cylinder, holding the short edges together with a
staple or paper clip and stand (the paper!) in 3/8" of water in a
suitable lidded container. 15-20 minutes later you will have a
colourful chromatographic separation of the main dyes in your samples.
Get the kids involved - they'll love it.

Chris

From daemon Fri May 3 13:59:22 2002

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Invitation and Call for Papers.

The First International Conference on Biomedical Spectroscopy:
} From Molecules to Men
7-10 July, 2002
Cardiff, Wales, United Kingdom

This conference covers spectroscopic as well as application of microscopic
techniques, such as AFM, for analysis of biological/biomedical systems.

Information regarding the conference is given below. You can also visit the
web-site of the conference:

http://www.dmu.ac.uk/In/biospectra/

Best wishes,
Dr P.I. Haris
Editor-in-chief: Spectroscopy - An International Journal
Editor: Biochemical Journal
Department of Biological Sciences,
De Montfort University, The Gateway, Leicester,
LE1 9BH, United Kingdom
E-Mail: pharis-at-dmu.ac.uk

First International Conference on Biomedical Spectroscopy:
} From Molecules to Men
7-10 July, 2002
Cardiff, Wales, United Kingdom

Aims and Scope of the Conference:

The first international conference on Biomedical Spectroscopy will bring
together spectroscopists and life scientists, from academia and
industry, engaged in solving problems of biological and biomedical
interest using diverse spectroscopic techniques. The conference is
dedicated to the memory of Professor Dennis Chapman FRS who introduced
many spectroscopic techniques to the study of biological systems.
The interdisciplinary nature of the conference will encourage scientific
interchange and cross-fertilisation of ideas.

Application of spectroscopic techniques such as
Mass spectrometry, CD, FTIR, NMR, EPR, ENDOR, NIR, SPR, Raman, EXAFS,
UV/Visible, Atomic Force Spectroscopy, Neutron Spectroscopy, Dielectric
Spectroscopy, Fluorescence Spectroscopy and so on,
for solving problems in diverse areas of life science including
-Biomedical and Clinical Science
-Biochemistry and Biophysics
-Biomaterials and Biosensors
-Proteomics and Pharmaceuticals
-Biotechnology and Biomedical Engineering

Call for Papers:

Scientists employing spectroscopic techniques in any area of life
science research are invited to submit proposals for lectures and poster
presentations. Papers presented at the conference will be reviewed and
published in a special issue of Spectroscopy - An International journal
(http://www.iospress.nl/site/navfr/navframe2.html)

Programme Structure:

The scientific programme will consist of invited lectures, as well as
contributed lectures selected from submitted contributions. There will
also be poster periods and time allocated for exhibition where
participants can discuss with spectroscopic manufacturers.

Deadlines:

Early Registration: 31 May 2002
Abstract Submission: 15 June 2002
Manuscript Submission for Publication in Spectroscopy: 8 July 2002

Conference Organising Committee Members :

Jose Luis Arrondo (University Pais Vasco, Spain)
Yoshinori Asakawa (Tokushima Bunri University, Japan)
Laurence D, Barron, (University of Glasgow, UK)
Andreas Barth (Johann Wolfgang Goethe Universitaet, Germany)
Lawrence J. Berliner (University of Denver, USA)
William Bonfield (University of Cambridge, UK)
Kevin M. Brindle (University of Cambridge, UK)
Chris Cooper (University of Essex, UK)
Gerard Cote (Texas A&M University, USA)
Lesley Davenport (CUNY, USA)
Martyn C Davies (University of Nottingham, UK)
Michael J. Davies (Heart Research Institute, Australia)
Peter J. Derrick (University of Warwick, UK)
David Gadian (Institute of Child Health, UK)
Parvez I. Haris (De Montfort University, UK)
Marcus A. Hemminga (Wageningen University, The Netherlands)
Larry Hench (Imperial College, London, UK)
Jan Johansson (Karolinska Institute, Stockholm, Sweden)
Mikio Kataoka (Nara Institute of Science & Technology, Japan)
Katrin Kneipp (Massachusetts Institute of Technology, USA)
Wlfgang Knoll (Max-Planck-Institut f�r Polymerforschung, Germany)
Kunihiro Kuwajima (University of Tokyo, Japan)
James M. McDonnell (University of Oxford, UK)
Henry H. Mantsch (Institute for Biodiagnostics, Canada)
Jeremy Nicholson (Imperial College, London, UK)
Niels Chr. Nielsen (University of Aarhus, Denmark)
Eric Oldfield (University of Illinois, USA)
Yukihiro Ozaki (Kwansei-Gakuin University, USA)
Hazime Saito (Himeji Institute of Technology, Japan)
Zhifeng Shao (University of Virginia School of Medicine, USA)
Gary Siuzdak (The Scripps Research Institute, USA)
Thomas G. Spiro (Princeton University, USA)
Gordon Tollin (University of Arizona, USA)
Bonnie Wallace (University of London, UK)

Conference Chair:

Dr P.I. Haris
Editor-in-Chief: Spectroscopy - An International Journal
http://www.iospress.nl/site/html/07124813.html
Editor: Biochemical Journal
http://www.biochemj.org/bj/bjedboard.htm
Editorial Advisor: Molecular Membrane Biology
http://www.tandf.co.uk/journals/boards/tmmb-edbrd.html
Treasurer: Protein and Peptide Science Group of Biochemical Society &
RSC
http://www.biochemistry.org/groups/ppsg/members.htm

Further information regarding the conference should be addressed to:

Dr Parvez I. Haris
Department of Biological Sciences,
De Montfort University, The Gateway, Leicester,
LE1 9BH, United Kingdom
E-Mail: pharis-at-dmu.ac.uk
Tel. 00-44-116-2506306 Fax: 00-44-116-2577287

REGISTRATION FORM:

Please send completed form by e-mail (pharis-at-dmu.ac.uk) to the
Conference Chair or by regular post: Dr P.I. Haris, Department of
Biological Sciences, De Montfort University, The Gateway, Leicester, LE1

9BH, United Kingdom, Fax: 00-44-116-2577287

Family name: .......................
Initials: .......................
First names: .......................
Institution: ..................................................
Department: ...............................................
Address: ....................................................
City/Postal Code: .............
Country: ..................
Telephone: .......................
Fax: .......................
E-mail: ........................................
Accompanying Person: .......................................

REGISTRATION FEES:

Amount
..........Early Registration. Payment received before 31 May 2002
(�400)
..........Late Registration. Payment received after 31 May 2002: (�500)
..........Student (�300)
..........Accompanying Person (�250)*
..........Total amount

Registration fee covers the following:
Accommodation for three nights in single rooms
Conference dinner
Lunch, tea, coffee for the duration of the conference
Conference bag, as well as the Abstract and Programme Book.
*Accompanying person registration fee covers accommodation for three
nights, conference dinner, lunch, tea and coffee.

PAYMENTS:

Registration fee can be directly paid by bank transfer: transfer should
be made to: 'Biospectra' Account, Account Number 02786668, Sort Code:
30-94-97, Lloyds TSB Bank, Leicester High Street Banch, Leicester, LE1
9FS, UK. The payments must be made in Sterling Pounds. Please state
your name and address.

Alternatively the registration fee can be paid by cheque or
international money order in Sterling Pounds, made payable to
'Biospectra'. The cheque should be sent by registered mail to Dr P.I.
Haris, Department of Biological Sciences, De Montfort University, The
Gateway, Leicester, LE1 9BH, United Kingdom.

Please send your payments by one of the above methods as soon as
possible. After receipt of your payment e-mail conformation will be
forwarded.

Accommodation:

Costs associated with accommodation are included within the registration
fee. Accommodation will be in single rooms for three nights covering
7-10 July 2002. Anyone requiring accommodation for additional nights
should contact the conference organiser in advance.

Information Regarding Arrival/Departure:

Date of arrival ........ / .......... / 2002
Date of departure ........ / .......... / 2002
*Need to be met at Cardiff Central Station: Yes/No
*Need to be met at Cardiff International Airport: Yes/No

*Please indicate time of arrival at the station or the airport by
e-mail.

Abstract Submission:

Any person registered to attend the conference may submit one Abstract
where he/she is the main author. It is possible submit additional
Abstracts where he/she is not the main author. The Organising committee
will select some of the Abstracts for oral presentation. Please
indicate if you are willing to present an oral presentation, if
selected.

The Abstract must be on one page, be single spaced and fit into an A4
size portrait-oriented rectangular space. Times New Roman fonts should
be used throughout the entire text of the abstract, including the title
and author sections. The name of the author who will present the paper
at the congress should be underlined. The Abstract should be prepared in
MS WORD for Windows. Abstracts should be sent by e-mail to
pharis-at-dmu.ac.uk or by regular post to the following address:
Dr P.I. Haris, Department of Biological Sciences, De Montfort
University, The Gateway, Leicester, LE1 9BH, United Kingdom. Fax:
00-44-116-2577287

Conference Publication:

Papers presented at the conference will be published in a special issue
of Spectroscopy - An International Journal. Manuscripts should be
submitted to the organisers at the Conference in Cardiff. Although
there are no specific length restrictions, papers should be of a length
appropriate to the material presented at the Conference. All papers
will be reviewed and are expected to be of a standard expected for a
paper published by the Journal in a regular issue.

The manuscripts should be prepared according to the instructions
available at http://www.iospress.nl/site/html/07124813_ita.html
The manuscript should be submitted at the conference in a diskette
containing the electronic version of the manuscript, together with two
paper copies.

The deadline for submission of manuscripts for publication in
Spectroscopy - An International Journal is 8 July 2002.

Exhibition:

Space will be reserved for exhibition of diverse spectroscopic
instrumentation, software, accessories, scientific journals and related
books. Companies interested in participating should take immediate
contact with the conference office.

Transportation:

Please let us know your travel plans so appropriate arrangements can be
made to meet you in either Cardiff Central Station or at Cardiff
International Airport.

London Heathrow Airport:

London's Heathrow Airport is the recommended route of arrival by air
into the UK. From here you can travel directly to Cardiff by National
Express coach (approximately 3 hours 15 minutes) or take the Heathrow
Express (15 minutes) to London's Paddington station and catch a First
Great Western The train journey to Cardiff Central station takes
approximately 2 hours. Members of the organising committee will be
available to meet the particpants at the Cardiff Central station.
Please indicate your arrival plans in advance.

London Gatwick Airport:

You can travel to Cardiff directly by National Express coach
(approximately 4 hours 45 minutes). Alternatively, you can take the
Gatwick Express train to London's Victoria Station (30 minutes) and
transfer to Victoria coach station from which the journey time is
approximately 3 hours 10 minutes. Alternatively from Victoria, use the
underground "Circle" line to reach Paddington Station and continue by
train as above.

London Stansted and London Luton Airports:

Budget flights are available from Europe into these airports which both
have rail and coach services into central London. For more information
on travel options visit the Stansted and Luton airport websites.

Cardiff International Airport:

Cardiff International Airport which is situated 12 miles west of the
city centre in Rhoose. Its main international connections are via
Amsterdam (KLM), Brussels and Paris (British Airways). British Airways
flights from Aberdeen, Edinburgh and Glasgow also serve the airport, as
well as Ryanair and British Airways flights from Dublin. Members of the
organising committee will be available to meet particpants arriving at
the airport. Please indicate your arrival plans in advance.

Eurostar/Train:

Arrival by Eurostar is to London Waterloo station. There is a direct
Great Western Train service from London Waterloo to Cardiff Central
Station with an approximate journey of 3 hours 10 minutes.
Alternatively, you may transfer at Waterloo to the underground
"Bakerloo" line to London Paddington where you can take the train as
above or take a coach from the Victoria station. Members of the
organising committee will be available to meet particpants arriving at
the Cardiff Central Station. Please indicate your arrival plans in
advance.

Information about Cardiff and Wales:

You can find out more details about Wales from various guides including
the Welsh Tourist Board (http://www.visitwales.com).

Cardiff, the capital city of Wales, boasts a number of tourist
attractions and has an extensive shopping centre. The civic centre
provides an interesting walking tour with many classical buildings
including Cardiff City Hall. Nearby are Cardiff Castle and the
Millennium Stadium. The National Museum (http://www.nmgw.ac.uk/) and
Gallery is also very popular with visitors. There are many eating and
drinking establishments situated in the city centre, ensuring that there
is something for everyone. Virtual Cardiff
(http://www.virtualcardiff.co.uk/) provides an indication of the wide
variety entertainment taking place in the city. Cardiff Bay, a
regenerated dock area, is a pleasant place to stroll around and can be
reached by a short train journey or 30 minutes walk from Cardiff City
centre. Cardiff, the Capital city is a lively, interesting and safe
city.

Further afield:

There are many interesting attractions in South Wales attractions
details of which can be found in various web-sites including Welsh
Tourist Board (http://www.visitwales.com). and Data Wales
(http://www.data-wales.co.uk/).

Activities:

Wales provides a wide variety of activities details of which can be
found in a number of websites including the Welsh Tourist Board
(http://www.visitwales.com). and Data Wales
(http://www.data-wales.co.uk/)

Useful Links:

Information regarding Wales: http://www.visitwales.com
Information Regarding Wales: http://www.data-wales.co.uk/
For UK train time tables visit www.railtrack.com
To book UK rail tickets outside the UK visit www.britcities.com
To book UK coach tickets visit www.gobycoach.com

Climate and Other Facts:

As Wales has a "temperate" climate, it generally never gets very hot or
very cold. July should be one of the sunniest and warmest months of the
year. However, it is advisable to bring a light rainproof coat and a
sweater, just in case, but you might possibly be in T-shirts. If you are
venturing into the mountains you will certainly need warmer clothing.
You can get a better idea nearer the time from the UK Meteorological
office (http://www.meto.gov.uk/).

From daemon Fri May 3 14:05:18 2002

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Unfortunately that is the case. Someone posts damaging material on this (or
any other) list, it get's sent around to some people a few times, and
suddenly it is "the official opinion of the microscopy specialists". I am
sure that can do substantial damage.

And it's not limited to the web or list servers. Audi (the German car
manufacturer) had a good business going in the US until someone decided to
start the engine and put it in forward (or backwards, I don't remember) and
run the car into a wall. There was a flaw, that allowed users to put the car
in gear without stepping on the brakes. Although that definitely was a flaw,
it was blown out of proportion and almost killed the Audi business in the
US. It took them 10 years to get their reputation back, even though I would
not consider this as dangerous as the tire problem that Firestone/Ford was
experiencing last year.

Bottom line: Everybody should be careful and check their sources when
posting potentially dmaging material on the listserver.

But that's just my 2 cents as a vendor.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Friday, May 03, 2002 6:40 AM
To: Microscopy-at-sparc5.microscopy.com

It's strange to see that one anonymous message could make so much damage to
the company. It seems to me that reputation of the company depends more on
the quality of the products/service etc nor on the one anonymous
opinion. From another hand, I think, this ListServer mostly is a place for
'customers' to exchange not only scientific ideas but to help each other to
survive in this 'capitalistic' world. Information about wrongdoing may
help others do not make similar mistake or spend money on bad quality
product/service. This is sort of our 'protection' against bad quality
service/products providers. Again, single decision even published here
could not damage the reputation of the good company with long well
established record of the service to EM community. I know, there are
places on the Internet where you could leave your opinion about some
particular business/company - it's perfectly legal and many institutions
used that lists for decision making. This discussion is more about ethics
- we all agree that we have rights openly speak here and others have the
rights to know who is speaking.

Sergey spoke.

At 02:15 AM 5/3/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Fri May 3 15:36:29 2002

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Obviously, one can only conclude that the person(s) who sent this email are
competitors of R�NTEC.

I am the owner of a small business & have always been in favor of fair
competition which benefits everyone.
Occasionally, we have someone resort to "badmouthing" us in order sway an
order.
I have never felt it necessary as we have always had an abundance of
business: relying on technical superiority & great customer relations.

I have found that equipment and Companies usually reflects the "personality"
of the people involved.
The same traits that I attribute to equipment (reliable, stable, solid)
reflects the personality of the people.
If the truth be known, I wonder about the integrity & reliability of
equipment the "anonymous Sender" may market.
It is obvious that he cannot sell based upon the equipment's technical
merits.

It is ashamed that a member of this Listserver would find it necessary to
resort to this "low-life" tactics.
I wonder if the anonymous Sender can look with pride at his accomplishments
& really take pride in himself for this feat.

My real name & my real Company for twenty years,

Earl Weltmer
Scanservice Corporation
----- Original Message -----
} From: Thomas Sch�lein
To: Microscopy-at-sparc5.microscopy.com
Sent: Friday, May 03, 2002 2:15 AM

Hello microscopists,
I am looking for a tried and true protocol for mouse bone. We are
interested in looking at the osteoblasts, and I am not sure how to
preserve them for examination of ultrathin sections in the TEM.
What is the best decalcification method? What type of resin for
embedding? Would a low viscosity resin, such as Spurr's, be the best?
I would appreciate any help you could give.
Thanks,
Jo Dee Fish

************************************************************
Jo Dee Fish
Research Technologist II
Microscopy Core
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 695-3720
Fax: (415) 285-5632
E-mail: jfish-at-gladstone.ucsf.edu

Mailing address:
Gladstone Institutes
P.O. Box 419100
San Francisco, CA 94141-9100
************************************************************

From daemon Fri May 3 18:26:36 2002

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Good morning everyone,

I am trying to round up a copy of a users manual for the following software:

HP Data Translation DT VEE 3.0

(The latest version of this software is now called AGLIENT VEE Pro.)

If anyone can help me out, it would be much appreciated.

Thanks!

John

John A. Rotole, Ph.D.
Project Engineer
Coatings & Surface Technology
Ispat Inland Product Research
3001 E. Columbus Drive
East Chicago, Indiana 46312
Tel: 219-399-6308
Fax: 219-399-6562

From daemon Fri May 3 18:52:09 2002

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At 01:28 PM 5/3/2002, you wrote:

[snip]

They were not totally anonymous since they had
a real(?) email address. A posting inquiry to that address
did not generate any response. So your point is
well taken.

gg

From daemon Fri May 3 22:23:23 2002

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Folks;

On a few occasions I have been handed reports from outside our organization
with EDX spectra that were saved as image files, e.g. .bmp. .tif, .jpg etc.
and pasted into a Word document or another report format. However, this
"cut and paste job" is only an image and in my mind isn't data at all but
simply information that can't be verified or refuted. A case in point is
when I received a spectra that only displayed energies up to 3.5 KeV and the
report identified Pt as Au which overlap at the lower energy region but
spread out higher up. Now without the raw data I cannot do anything but
take someone's word or politely interrogate the person that did the
analysis. My question is whether it would be appropriate to ask someone on
this listserver with the same EDX system to look at the raw data, if
available, provided they have the same platform?

These matters are generally not life and death like a forensics issue but
purely economic, but I'd rather be in jail than lose money.

Regards,
Peter

From daemon Sat May 4 08:17:26 2002

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Peter

The task of sharing spectra cross-platform was address a number
of years ago by the MSA/MAS Standards Committee.

You could ask the individual to send the raw data to you
in the MSA/MAS File format. You can then look at it on
any computer with something as simple as a spread sheet
program. In addition, a number of commerical manufacturers
have implemented a routine to read/write data to/from this
format, hence you might be able to look at the raw data on
your own system.

You can find the details on the MSA/MAS format for Spectra at

ftp://www.amc.anl.gov/AMC-3/ANLSoftwareLibrary/2-EMMPDL/Eels/emmff/

Nestor
Your Friendly Neighborhood SysOp

========================
Keywords :XEDS,EELS,AES,WDS,CLS,GAM,XRF,PES
Computer :IBM, MAC, DEC
Operating System :ALL
Programming Language :Fortran 77
Hardware Requirements :None
Author(s) :EMSA/MAS TASK FORCE
Ray Egerton ,Charles E. Fiori ,John A. Hunt,
Mike S. Isaacson,Earl J. Kirkland ,Nestor J. Zaluzec
Correspondence Address :R.F. EGERTON-CHAIRMAN
University of Alberta
Dept. of Physics
Edmonton, Alberta, Canada, T6G2J1
Abstract:

A simple format for the exchange of digital spectral data is
presented, and proposed as an EMSA/MAS standard. This format is readable by
both humans and computers and is suitable for transmission through various
electronic networks (BITNET, ARPANET), the phone system (with modems) or on
physical computer storage devices (such as floppy disks). The format is not
tied to any one computer, programming language or computer operating system.
The adoption of a standard format would enable different laboratories to freely
exchange spectral data, and would help to standarize data
analysis software. If equipment manufacturers were to support a common format,
the microscopy and microanalysis community would avoid duplicated effort in
writing data-analysis software. This version of EMSAMASFF contains two
subroutines which read and write spectral data files Version 1.0 data format.
The data are stored as simple ASCII characters at a user defined number of
columns per line for the length of the data file. The spectral data is
preceeded by a series of header lines, which tell the user about the
parameters of the spectrum. The header lines are identified by the first
character in the line being the symbol (#) followed by a descriptor and if
appropriate its units. An example of a data file format can be found in the
EMSAMASFF.DOC file.

From daemon Sat May 4 11:56:04 2002

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I use EDTA with NaOH to decalcify, it's slow and you have to keep it cold,
but it is gentle. Then dehydrate and infiltrate with long spells in each
change, and embed in a hard resin - I use Epon with DDSA and NMA and, to
embed, 2% DMP30. Use vacuum or a rotator (or both in turn) to force the
resin in, and after embedding leave it overnight at 37C, if possible under
vacuum (I use an old paraffin oven) before putting it into the 60C oven.
People do use microwave techniques, but I never had much luck with them.
Hope this helps.

Lesley Weston.

on 03/05/2002 1:36 PM, Jo Dee Fish at jfish-at-gladstone.ucsf.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello microscopists,
} I am looking for a tried and true protocol for mouse bone. We are
} interested in looking at the osteoblasts, and I am not sure how to
} preserve them for examination of ultrathin sections in the TEM.
} What is the best decalcification method? What type of resin for
} embedding? Would a low viscosity resin, such as Spurr's, be the best?
} I would appreciate any help you could give.
} Thanks,
} Jo Dee Fish
}
} ************************************************************
} Jo Dee Fish
} Research Technologist II
} Microscopy Core
} Gladstone Institute of Cardiovascular Disease
}
} Telephone: (415) 695-3720
} Fax: (415) 285-5632
} E-mail: jfish-at-gladstone.ucsf.edu
}
} Mailing address:
} Gladstone Institutes
} P.O. Box 419100
} San Francisco, CA 94141-9100
} ************************************************************
}

From daemon Sat May 4 15:49:35 2002

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I think that you have a need to trust your analyst to know what he/she is doing. Unfortunately, in the age of push button data collection on instruments that anyone can use, the training may or may not be up to snuff. Of course, you can rely on "certified" labs that are ISO-9000 qualified, but if you do, they may be just following a rote script. It is best to know that analyst's pedigree and their experience level.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Friday, May 03, 2002 11:20 PM
To: Microscopy-at-sparc5.microscopy.com

Folks;

On a few occasions I have been handed reports from outside our organization
with EDX spectra that were saved as image files, e.g. .bmp. .tif, .jpg etc.
and pasted into a Word document or another report format. However, this
"cut and paste job" is only an image and in my mind isn't data at all but
simply information that can't be verified or refuted. A case in point is
when I received a spectra that only displayed energies up to 3.5 KeV and the
report identified Pt as Au which overlap at the lower energy region but
spread out higher up. Now without the raw data I cannot do anything but
take someone's word or politely interrogate the person that did the
analysis. My question is whether it would be appropriate to ask someone on
this listserver with the same EDX system to look at the raw data, if
available, provided they have the same platform?

These matters are generally not life and death like a forensics issue but
purely economic, but I'd rather be in jail than lose money.

Regards,
Peter

From daemon Sat May 4 17:32:32 2002

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Scott;

Unfortunately sometimes the "analyst" is a few people removed in the food
chain of the organization I may have to deal with and that's sometimes a
customer so I must at all times be cordial and diplomatic. It turned out
that the individual that sent me the "pictorial" spectra, but no raw data,
was a "push button" let the machine identify everything type of analyst and
was not very conversant in where error comes from in EDX analyses. He also
had no real understanding of the device he was "analyzing" so I should
probably be a bit more forgiving. I would have preferred that his report
stated "these are the possible elements detected, Au, Pt etc."

I must take this opportunity to agree with you on ISO9000 certification and
speak my mind. ISO is not an education in engineering or science, it is an
education on how to be compliant with paperwork. If one documents a bad
recipe for a cake, one will get a consistently bad cake but will not be on
The Martha Stewart Show. I often wonder how many organizations filed for
bankruptcy but had wonderful ISO documentation and passed all their audits?
I really should look this up somewhere.

I hope my boss doesn't subscribe to this, naaaaah.

Peter

-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Saturday, May 04, 2002 4:25 PM
To: 'Peter Tomic'
Cc: Microscopy (E-mail)

Folks;

On a few occasions I have been handed reports from outside our organization
with EDX spectra that were saved as image files, e.g. .bmp. .tif, .jpg etc.
and pasted into a Word document or another report format. However, this
"cut and paste job" is only an image and in my mind isn't data at all but
simply information that can't be verified or refuted. A case in point is
when I received a spectra that only displayed energies up to 3.5 KeV and the
report identified Pt as Au which overlap at the lower energy region but
spread out higher up. Now without the raw data I cannot do anything but
take someone's word or politely interrogate the person that did the
analysis. My question is whether it would be appropriate to ask someone on
this listserver with the same EDX system to look at the raw data, if
available, provided they have the same platform?

These matters are generally not life and death like a forensics issue but
purely economic, but I'd rather be in jail than lose money.

Regards,
Peter

From daemon Sat May 4 19:07:50 2002

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At 12:01 PM -0700 on 5/3/02 you wrote:
} Hi All:
} I am trying to convert an image and spectra from the Oxford AN 10000 EDS
} to a .tiff that is readable on a PC. I've done it in the past, but it has
} been so long, I forget the exact procedure. I hope that there is
} somone out there that does this more regularly that can refresh my
} memory.
} Thanks in advance,
} Mike Coviello
} UT Arlington

Procedure for converting AN10000 spectrum screendumps to TIFF format
files on PC DOS disks

1) From the Analyser MAIN MENU select (4) PRINT/PLOT

2) Select (3) DUMP TO DISK
Enter filename FILENAME.IM
Message: File FILENAME.IM does not exist in STAFF/STUDENT.DR
Do you wish to create it? Press Y
The spectrum will now be saved to the working directory as FILENAME.IM

3) Return to the GO menu

4) Select (4) FILE MANAGEMENT

5) Select (16) DEMON/TIFF convert

6) Select (1) IMAGE filename. Enter FILENAME.IM

7) Select (2) TIFF filename. Enter FILENAME.TI

8) Select (3) LOOKUP table: MSDOSCV.LT

9) Select (8) IMAGE to TIFF
Overwrite file if it exists: Yes
Rescale pixels for grey image: Yes

10) Select (10) MSDOS convert
Insert DOS formatted disk in DM0
Source disk for DOS: DM0

11) Select (1) CONVERT files
Copy file to (MSDOS/DEMON): MSDOS
Filename please: FILENAME.TI
Any conversion type: B
Transfer will now take place, lasting approximately 3
minutes. The TIFF file will be a
grey-scale image. Use an image processing package to
change it to color.

12) Select (3) DEMON utilities

13) Select (2) DELETE DEMON files
Enter FILENAME.-
Message: Delete DEMON file FILENAME.TI? Press Y
Message: Delete DEMON file FILENAME.IM? Press Y
Press Blue

14) Select (20) EXIT

15) Select (20) EXIT

--
Barton Smith, Ph.D.
Advanced Lasers and Optics Group
Engineering Science and Technology Division
Oak Ridge National Laboratory

From daemon Sun May 5 04:22:02 2002

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If your opinion is being sought, then you should have available all raw
data. Whether it is a legalistic matter or not, a definitive opinion can
not be rendered on a synopsis of available data, only on original data.

Preferably, the original sample would be made available to you for
analysis. In this way, not only the interpretation, but also the
providence of that interpretation would be verifiable by you.

Your query whether it is appropriate to find someone with the same
equipment is moot. It is the interpretation of the results given the
analytical conditions that is far more important than the data delivered.
The data as delivered to you has already undergone the filters of the one
who sent it to you. You need to see past that and provide your own
interpretation of the truth, which can only be based on data that you know
to be based on some semblance of reality. Since the data collection of EDX
spectra is based on a variety of experimental conditions, the better they
are known, the better they can be corrected for.

In other words, you need to know not only the raw data but also the
experimental conditions it was collected under as well as the providence of
the sample. To provide an opinion on anything less would be a disservice
to those who ask as well as those who oppose the requesting party.
Assuming the desire to be an impartial source, there is no other course
than to do your own analysis..

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com

}
} Folks;
}
} On a few occasions I have been handed reports from outside our
organization
} with EDX spectra that were saved as image files, e.g. .bmp. .tif, .jpg
etc.
} and pasted into a Word document or another report format. However, this
} "cut and paste job" is only an image and in my mind isn't data at all but
} simply information that can't be verified or refuted. A case in point is
} when I received a spectra that only displayed energies up to 3.5 KeV and
the
} report identified Pt as Au which overlap at the lower energy region but
} spread out higher up. Now without the raw data I cannot do anything but
} take someone's word or politely interrogate the person that did the
} analysis. My question is whether it would be appropriate to ask someone
on
} this listserver with the same EDX system to look at the raw data, if
} available, provided they have the same platform?
}
} These matters are generally not life and death like a forensics issue but
} purely economic, but I'd rather be in jail than lose money.
}
} Regards,
} Peter
}

From daemon Sun May 5 06:57:11 2002

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Karli - by chance could your column be containmented or have a whisker?
I know sometimes if your image isn't good sometimes your filament could
be going, but probably not for weeks. Just a thought.
Barb

From daemon Sun May 5 14:35:37 2002

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Disclaimer: This is not a for sale posting.

I ran across an LSI-11 bus M7555 card which
seems to be in good condition. No idea what it
is or if it works. It has a ribbon connector (1)
on the pull handles end. It is a DEC card.

Free to a good home.

gary g.

From daemon Sun May 5 18:55:03 2002

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Allen;

Thank you. I am saving this email for reference in my next argument with
upper management.

Peter

-----Original Message-----
} From: Allen Sampson [mailto:ars-at-sem.com]
Sent: Sunday, May 05, 2002 7:11 AM
To: 'Peter Tomic'; Microscopy-at-sparc5.microscopy.com

Hi all,

I have a specific question regarding experiments in inert environment:

The experiment I did was: A small superalloy (SA) sample was taken
in a quartz tube and I placed the tube horizontally in a tube furnace. I
then ran Ar gas for a few minutes through it and under the assumption
that all oxygen is gone, heated the tube to 1200 C, held there for 50
min before cooling the apparatus to 200C. This I thought would avoid
any oxidation of the SA sample.
On cooling, to my disappointment, the SA surface
oxidized (shiny surface became black). In fact, the quartz tube became
black from inside. Note that throughout the experiment, I had inserted
a thermocouple in the quartz tube, which (the thermocouple) had a Nextel
Ceramic fiber insulation. This insulation also turned black.

The specific questions in my mind are:
1) How do I make sure that all the oxygen is flushed out? Is a
vacuum necessary before Ar gas is filled in the tube?
2) In my experiment, did the Thermocouple insulation emit any gas (from
the black color of the quartz tube and the nextel insulation)?
In that case, can i have a bare theomocouple wire in the tube at
1200C?

Thanks in advance

Rahul Panat
Dept. of Theoretical and Applied Mechanics
Univ of Illinois,
Urbana, IL

From daemon Sun May 5 22:50:44 2002

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on 5/5/02 10:11 PM, rahul padmakar panat at panat-at-students.uiuc.edu wrote:

}
} I have a specific question regarding experiments in inert environment:
}
} The experiment I did was: A small superalloy (SA) sample was taken
} in a quartz tube and I placed the tube horizontally in a tube furnace. I
} then ran Ar gas for a few minutes through it and under the assumption
} that all oxygen is gone, heated the tube to 1200 C, held there for 50
} min before cooling the apparatus to 200C. This I thought would avoid
} any oxidation of the SA sample.
} On cooling, to my disappointment, the SA surface
} oxidized (shiny surface became black). In fact, the quartz tube became
} black from inside. Note that throughout the experiment, I had inserted
} a thermocouple in the quartz tube, which (the thermocouple) had a Nextel
} Ceramic fiber insulation. This insulation also turned black.
}
} The specific questions in my mind are:
} 1) How do I make sure that all the oxygen is flushed out? Is a
} vacuum necessary before Ar gas is filled in the tube?
} 2) In my experiment, did the Thermocouple insulation emit any gas (from
} the black color of the quartz tube and the nextel insulation)?
} In that case, can i have a bare theomocouple wire in the tube at
} 1200C?
}
} Thanks in advance
}
} Rahul Panat
}
Dear Rahul,
If you had a long, thin quartz tube, the Ar would not necessarily flush
out all the O2, so, yes, I would evacuate the tube furnace, flush with Ar,
repeat until all the O2 is gone. (I'm thinking that the tube was not open
at both ends, and that the Ar was not directed at the end of the tube.) I'd
try the experiment again without the SA, but with a clean quartz tube and
new TC; if they again turn black, then it's something in the TC insulation.
If not, try again with the SA, and see if anything unexpected occurs. Good
luck.
Yours,
Bill Tivol

From daemon Mon May 6 00:51:18 2002

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Dear Ann Fook

We normally imbed in gelatin capsules. Polymerise in a oven. Use a hacksaw
(Very small) to cut and re-glue our specimens in the preferred orientation
before sectioning. That way our orientation is perfect every time!

Just a useful hint.

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana

Phone : +267 355 2426
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw

-----Original Message-----
} From: Ann-Fook Yang [mailto:yanga-at-EM.AGR.CA]
Sent: Friday, May 03, 2002 5:26 PM
To: microscopy-at-sparc5.microscopy.com

Hi everyone,

I need to do flat embedding using LR White. A Teflon mold covered with
ACLAR seems to be the answer. I am afraid that the over filled resin under
ACLAR may not polimerize properly and become messy. I appreciate your
experience.

Ann Fook Yang

From daemon Mon May 6 01:54:04 2002

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Dear all,

We have a Jeol 100CX TEM that, I am told, has developed the following
vacuum problem over the last 5-7 years. The problem, whatever its cause,
means that the viewing port into the observation chamber has to be removed
once a month or so to clean off the buildup of oil on the inside. I am
amazed that this was considered Ok for the microscope........ So, my
proposal is to replace all the rubber vacuum lines, and clean out, as best
as possible, the remaining components - metal hoses, etc. We're not sure
of the cause of this problem, whether it's oil from the rotary pumps or
from one of the diffusion pumps. Oh, and we need to clean, or at least
check, the column as well, of course......

My questions are:
Can we replace the original rubber tubing with wire-reinforced clear PVC
tubing (at least then we'd see any oil buildup in future)? It's a little
unclear how much vacuum the PVC tubing will take and this will be important
in the internals of the microscope.

How can we identify the source of the problem? I'm proposing to put
foreline traps on both rotary pumps, but if a diff pump is the problem,
this won't solve it. I'm also proposing to take out the diff pumps and
check them, change the oil, etc. I've had a look through Will Bigelow's
Vacuum Methods book (very useful!), and it's a bit like reading an index of
diseases and their symptoms - you think you have a whole variety of them!!
- I can see several incidents and problems that could have affected a
number of the vacuum components.

Thanks for any and all advice!
cheers,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au

From daemon Mon May 6 05:13:01 2002

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Yes, a changeover to the clear PVC hosing is appropriate - I love the
stuff. The problem you are experiencing is probably more a consequence of
the calibration of the vacuum system than anything else.

Anyone who has been here for awhile has probably seen postings from me on
the vacuum system calibrations of EMs regarding the cross-over points of
diffusion pumped systems. Too often, design engineered or field
engineering solutions result in a cross-over vacuum level from roughing to
diffusion pumps at too high a pressure level. When a diffusion pump is
opened to a pressure level too high, it can stall which results in the
breakdown of the normal laminar flow of oil vapor to a chaotic flow which
results in the influx of oil vapor into the sample chamber.

Your first step should be to determine the actual chamber pressure at which
the cross-over from roughing to diffusion pump occurs. At the least, you
can get a qualitative determination from the action of the chamber vacuum
when the diffusion pump kicks in. At that point, the vacuum level should
start a rapid increase to the ultimate vacuum. If, instead, the vacuum
level declines or holds steady for a few seconds, the cross-over point is
too high and the diffusion pump is stalling. Cross-over should normally
occur at 70 - 100 microns, most diffusion pumps will react well in this
area. I generally tend to set the cross-over at 70 microns - it will take
longer for the roughing time but when the diffusion pump kicks in it will
rapidly pull down to the ultimate vacuum and the sample chamber will remain
cleaner.

Field engineers don't normally carry independant vacuum measuring gear.
Instead, they depend on known timing characteristics of known good
systems. If your system doesn't match the characteristics expected, the
calibration will be off. The only offset to this is the use of a leak back
test, where the actual leak rate of the system can be tested. If the leak
back can be verified as within the manufacturer's specs, then the
calibration can be applied and expected to achieve specs.

If, however, there is no absolute measurement made of either actual vacuum
levels or actual leak rates, then no assumptions can possibly be made of
the vacuum levels achieved. Turbo-pumping systems actually make this
determination of vacuum levels more problematic as they are seldom capable
of closing a valve on the sample chamber to allow the determination of its
leak rate, making the use of accurate vacuum level measuring equipment
neccessary to determining the the calibration of system levels.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com

On Sunday, May 05, 2002 11:50 PM, Rosemary White
[SMTP:rosemary.white-at-csiro.au] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} We have a Jeol 100CX TEM that, I am told, has developed the following
} vacuum problem over the last 5-7 years. The problem, whatever its cause,
} means that the viewing port into the observation chamber has to be
removed
} once a month or so to clean off the buildup of oil on the inside. I am
} amazed that this was considered Ok for the microscope........ So, my
} proposal is to replace all the rubber vacuum lines, and clean out, as
best
} as possible, the remaining components - metal hoses, etc. We're not sure
} of the cause of this problem, whether it's oil from the rotary pumps or
} from one of the diffusion pumps. Oh, and we need to clean, or at least
} check, the column as well, of course......
}
} My questions are:
} Can we replace the original rubber tubing with wire-reinforced clear PVC
} tubing (at least then we'd see any oil buildup in future)? It's a little
} unclear how much vacuum the PVC tubing will take and this will be
important
} in the internals of the microscope.
}
} How can we identify the source of the problem? I'm proposing to put
} foreline traps on both rotary pumps, but if a diff pump is the problem,
} this won't solve it. I'm also proposing to take out the diff pumps and
} check them, change the oil, etc. I've had a look through Will Bigelow's
} Vacuum Methods book (very useful!), and it's a bit like reading an index
of
} diseases and their symptoms - you think you have a whole variety of
them!!
} - I can see several incidents and problems that could have affected a
} number of the vacuum components.
}
}
} Thanks for any and all advice!
} cheers,
} Rosemary
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} 61- 2 6246 5475 or
} 61- 0402 835 973
} rosemary.white-at-csiro.au
}
}
}
}
}

From daemon Mon May 6 06:04:52 2002

Contents Retrieved from Microscopy Listserver Archives
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From daemon Mon May 6 08:09:04 2002

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Dear Microscopists & Co.;

I received the below notice from our friendly IT people. I believe it was
incoming from the listserver and I though you may want to be cautious about
the "sender."

Regards,
Peter

-----Original Message-----
} From: MB10190 [mailto:MB10190-at-aol.com]
Sent: Sunday, May 05, 2002 1:40 PM
To: PTomic-at-anadigics.com

I would add; verify purity of your argon or use an argon purifier.

At 8:40 PM -0400 5/5/02, Bill & Sue Tivol wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html

From daemon Mon May 6 09:14:22 2002

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Ann-Fook,

My approaches in using LRWhite differ by the nature of the sample being
embedded:
Hard samples, such as silica and zeolites, are embedded in LRWhite (hard
grade) without accelerator. The sample is first vacuum degassed,
immersed in LRWhite in the mold and placed under vacuum again to
facilitate complete infiltration of the resin into the sample. The
embedded sample is then cured overnight at 75-90C. This gives a hard,
brittle block that is ideal for sectioning hard samples.
I try to avoid embeddment of polymers in LRWhite. Some polymers may be
partially to very soluble in the acrylic monomer and thus susceptible to
swelling by the resin. Furthermore, since the heat of curing is not
known to me, I choose not to take chances on the annealing samples
during exothermic curing. Rather, I generally choose to use one of the
generic Epon 812 replacements for these materials. Carefully controlled
curing conditions allow embedment and microtomy without concerns of
annealing.
This said, if I must embed polymers in LRWhite, I choose the correct
hardness of resin (soft, medium or hard) for the material being
embedded. The embedding mold is degassed for a bit under a nitrogen flow
and the mold surfaces are then wiped with the accelerant on a cotton
swab. This helps the resin in contact with the mold surface to more
fully cure. The samples are embedded in the resin/accelerator and
immediately placed under nitrogen flow until curing is complete.

Good luck,

Gary M. Brown

"Ann-Fook Yang"
{yanga-at-EM.AGR.C To: {microscopy-at-sparc5.microscopy.com}
A} cc:
Subject: Flat embedding of LR White

05/03/02 10:26
AM

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hi everyone,

I need to do flat embedding using LR White. A Teflon mold covered with
ACLAR seems to be the answer. I am afraid that the over filled resin under
ACLAR may not polimerize properly and become messy. I appreciate your
experience.

Ann Fook Yang

From daemon Mon May 6 09:42:07 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm glad someone else got around to this before I did! As
you can see, it's rather involved. I will add two
additional points:

1) One of my AN10000's doesn't have DEMON/TIFF convert as
an option (older software). I must use the "newer" version
of the QX200 Utilities software.
2) At step 9 below: both answers must be an uppercase 'Y'.
Again, possibly peculiar to my system/version.
Good luck!

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu

On Saturday, May 04, 2002 7:58 PM, Barton Smith
[SMTP:smithdb-at-ornl.gov] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} ml
}
----------------------------------------------------------
} -------------.
}
}
} At 12:01 PM -0700 on 5/3/02 you wrote:
} } Hi All:
} } I am trying to convert an image and spectra from the
} } Oxford AN 10000 EDS
} } to a .tiff that is readable on a PC. I've done it in
the
} } past, but it has
} } been so long, I forget the exact procedure. I hope that
} } there is
} } somone out there that does this more regularly that can
} } refresh my
} } memory.
} } Thanks in advance,
} } Mike Coviello
} } UT Arlington
}
}
} Procedure for converting AN10000 spectrum screendumps to
} TIFF format
} files on PC DOS disks
}
} 1) From the Analyser MAIN MENU select (4) PRINT/PLOT
}
} 2) Select (3) DUMP TO DISK
} Enter filename FILENAME.IM
} Message: File FILENAME.IM does not exist in
} STAFF/STUDENT.DR
} Do you wish to create it? Press Y
} The spectrum will now be saved to the working directory
} as FILENAME.IM
}
} 3) Return to the GO menu
}
} 4) Select (4) FILE MANAGEMENT
}
} 5) Select (16) DEMON/TIFF convert
}
} 6) Select (1) IMAGE filename. Enter FILENAME.IM
}
} 7) Select (2) TIFF filename. Enter FILENAME.TI
}
} 8) Select (3) LOOKUP table: MSDOSCV.LT
}
} 9) Select (8) IMAGE to TIFF
} Overwrite file if it exists: Yes
} Rescale pixels for grey image: Yes
}
} 10) Select (10) MSDOS convert
} Insert DOS formatted disk in DM0
} Source disk for DOS: DM0
}
} 11) Select (1) CONVERT files
} Copy file to (MSDOS/DEMON): MSDOS
} Filename please: FILENAME.TI
} Any conversion type: B
} Transfer will now take place, lasting approximately 3
} minutes. The TIFF file will be a
} grey-scale image. Use an image processing
} package to
} change it to color.
}
} 12) Select (3) DEMON utilities
}
} 13) Select (2) DELETE DEMON files
} Enter FILENAME.-
} Message: Delete DEMON file FILENAME.TI? Press Y
} Message: Delete DEMON file FILENAME.IM? Press Y
} Press Blue
}
} 14) Select (20) EXIT
}
} 15) Select (20) EXIT
}
}
}
} --
} Barton Smith, Ph.D.
} Advanced Lasers and Optics Group
} Engineering Science and Technology Division
} Oak Ridge National Laboratory

From root Mon May 6 10:08:06 2002
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Dear Rahul Panat

If it is possible link the tube to a vacuum system it will help. Flushing
with Ar a few times will improve it dramatically. It is worth to allow a
very slow flow of Ar during heating and cooling over your sample. This is
done by closing both ends of your quarts tube with a inlet and outlet pipe.
The outlet pipe is run into a holder with water. Increase the Ar flow until
a very small bubble rate is achieved. This will ensure a inert atmosphere
with a positive Ar pressure.

Yours Sincerely
Stephan H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}

Hi all,

I have a specific question regarding experiments in inert environment:

The experiment I did was: A small superalloy (SA) sample was taken
in a quartz tube and I placed the tube horizontally in a tube furnace. I
then ran Ar gas for a few minutes through it and under the assumption
that all oxygen is gone, heated the tube to 1200 C, held there for 50
min before cooling the apparatus to 200C. This I thought would avoid
any oxidation of the SA sample.
On cooling, to my disappointment, the SA surface
oxidized (shiny surface became black). In fact, the quartz tube became
black from inside. Note that throughout the experiment, I had inserted
a thermocouple in the quartz tube, which (the thermocouple) had a Nextel
Ceramic fiber insulation. This insulation also turned black.

The specific questions in my mind are:
1) How do I make sure that all the oxygen is flushed out? Is a
vacuum necessary before Ar gas is filled in the tube?
2) In my experiment, did the Thermocouple insulation emit any gas (from
the black color of the quartz tube and the nextel insulation)?
In that case, can i have a bare theomocouple wire in the tube at
1200C?

Thanks in advance

Rahul Panat
Dept. of Theoretical and Applied Mechanics
Univ of Illinois,
Urbana, IL

From root Mon May 6 15:37:48 2002
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Pauline,
Try decreasing the brighter labels concentration. Also run single label
counterparts as bleed through
controls (same concentrations as the double label, but use only the fitc and
PI separately). If you get
bleed with the single label samples (capture with both channels), you can
then do a backround subtract
to get the real signal. Good luck

Mike D.

pcy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} We have stained annelid embryos with FITC-conjugated anti-tubulin antibody
} and propidium iodide. We are using a Biorad-MRC-600 and seem to be getting
} almost identical signals(of nuclei and a few mitochondria) on both the 488
} and 568 wavelengths, even though when viewed with epifluorescence, the
} tubulin stain seems to distinctly define cell borders. I understood that
} the propidium iodide emission would not be overlapping with that of
} FITC--is this incorrect? I have other double-labelled samples which are
} stained with different stains, but their signals are within the
} appropriate emission spectra for both confocal and epifluorescence, so the
} detection filters on the confocal do not seem to be the problem.
}
} Any feedback is appreciated!
}
} Pauline Yu
} pcy-at-usc.edu
} Manahan Lab
} http://www.usc.edu/manahanlab

From daemon Mon May 6 16:38:29 2002

Contents Retrieved from Microscopy Listserver Archives
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} Date: Mon, 06 May 2002 14:39:09 -0700
} To: "ars-at-sem.com" {ars-at-sem.com}
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: RE: cleaning vacuum system
}
} The only things you have to keep in mind: PVC tubes are less flexible and
} may 'transfer' vibration from rotary pump to the microscope's column. For
} this reason, I love to use PVC on vacuum evaporators nor the microscopes.
}
} As for oil in the column - to me it looks like DP problem and water
} waffler, which is situate over DP. The reason I think so, that usually we
} do not pump columns down by RP very often. It happening on my microscope
} a few times over 15 years, I believe. So, it mean, that RP do not touch
} column frequently (except terrible downstream if it happening). I do
} believe that 100C/CX DPs have water wafflers, check does water go
} through. Another things: if DP oil is old (or overheated over some period
} of time) it may be deteriorated and boiling point may decreased (shorter
} molecules), so some low-weight components may not condensate effectively
} and will migrate into the column. I would suggest to check DP oil and
} replace it on Santovak-5 like type (and forget for 5+ years). If you
} decided to replace DP oil, I would suggest, you install oil traps on RPs
} and new tubings as well to protect fresh oil (you never know...). Good
} luck! Sergey
}
} At 05:04 AM 5/6/02 -0700, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From daemon Mon May 6 16:47:33 2002

Contents Retrieved from Microscopy Listserver Archives
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After all the cleaning, I would check the water lines & the water
temperature.

I have had problems similar to the one you describe. It turned out that
after moving the EM the input & output lines were reversed causing oil
backstreaming. In the second case, the water temperature had increased
overnight as it was connected to the tap water.

Another thought would be to check the pre-pump pressure to ensure that it is
switching over at the correct pressure.

Regards,

Earl

----- Original Message -----
} From: "Rosemary White" {rosemary.white-at-csiro.au}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, May 05, 2002 11:49 PM

Dear Rosemary

Once, in the darkness of time (1975-...), I used one of the first JEOL 100C
microscopes. That machine also developed with time severe contamination
problems that, I believe, may be similar to the ones you mention.
As far as we could find out, and as far as I can recall (forgive some
possible innacuracies) the trouble came from the particular design of the
vacum system, in which two diffusion pumps are linked together, the exit of
the upper pump being connected to the observation chamber. In these early
microscopes, that design seemed to lead to oil reflux from the exit of the
upper pump into the column, at least during some stages of the vacum
sequence. One cure for the problem was to close permanently the valve
connecting the observation chamber with the vacuum system. That was the only
measure that worked! Enough vacuum could still be achieved by the upper
connection alone.
As far as I was told newer microscopes (yours should be one of these) were
built with an improved automated vacuum sequence and traps that avoided that
problem. My guess is that either your oil traps are not operational
(obstructed water circulation?), either the vacuum sequence is incorrect
(check the pirani gauge settings that control the sequence against true
vacum values if you are able to measure them!). Some vacuum valve (the lower
connection to the column should be the primary suspect) may be stuck, or
electronics is producing a wrong sequence etc...
Anyhow if you have this problem, continuos cleaning does not seem to be an
answer. It was not for me.

Best wishes and good luck
Prof. Doctor A.P. Alves de Matos
Anatomic Pathology Department
Curry Cabral Hospital
Lisbon

-----Mensagem original-----
De: Rosemary White [mailto:rosemary.white-at-csiro.au]
Enviada: Segunda-feira, 6 de Maio de 2002 7:50
Para: Microscopy-at-sparc5.microscopy.com
Assunto: cleaning vacuum system

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dear all,

We have a Jeol 100CX TEM that, I am told, has developed the following
vacuum problem over the last 5-7 years. The problem, whatever its cause,
means that the viewing port into the observation chamber has to be removed
once a month or so to clean off the buildup of oil on the inside. I am
amazed that this was considered Ok for the microscope........ So, my
proposal is to replace all the rubber vacuum lines, and clean out, as best
as possible, the remaining components - metal hoses, etc. We're not sure
of the cause of this problem, whether it's oil from the rotary pumps or
from one of the diffusion pumps. Oh, and we need to clean, or at least
check, the column as well, of course......

My questions are:
Can we replace the original rubber tubing with wire-reinforced clear PVC
tubing (at least then we'd see any oil buildup in future)? It's a little
unclear how much vacuum the PVC tubing will take and this will be important
in the internals of the microscope.

How can we identify the source of the problem? I'm proposing to put
foreline traps on both rotary pumps, but if a diff pump is the problem,
this won't solve it. I'm also proposing to take out the diff pumps and
check them, change the oil, etc. I've had a look through Will Bigelow's
Vacuum Methods book (very useful!), and it's a bit like reading an index of
diseases and their symptoms - you think you have a whole variety of them!!
- I can see several incidents and problems that could have affected a
number of the vacuum components.

Thanks for any and all advice!
cheers,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au

From daemon Mon May 6 18:20:45 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Listed below is the table of contents of the May/June issue of
MICROSCOPY TODAY (MT) which is the newest publication of
the Microscopy Society of America (MSA), the host of the
Microscopy Listserver.

As a service to the Microscopy Community and begining with the
May/June issue of MT the table of contents of MT, will be routinely
submitted to the Listserver.

Issues of Microscopy-Today are free, thanks to our advertisers, for any
individuals who receive delivery of their copies in the USA.
Non-USA subscribers who are also members of MSA will also receive
free subscriptions. Non-USA, non-MSA members, who wish to receive
copies of MT will find subscription rates on the MT web site
(http://www.microscopy-today.com). In the transition from the
previous publisher (Don Grimes) into an MSA publication, we
have found there were a number of non-USA, paid subscribers, who are
also MSA members. We plan to credit these folks appropriately.

The entire MSA membership has been added to the MT subscriber list and
as many duplicates as possible have been removed. We are left with
potential duplicates for the case where two records have the same name
but different addresses. If you have moved and did not change your
address or if you are receiving two copies, please let me know
by Email at: microtoday-at-attglobal.net.

I plan to close the subscription list and generate address labels for
the May/June issue on May 8th. People who subscribe after that date
will begin to receive their issues in July.

All subscriptions and address changes should go to
http://www.microscopy-today.com directly or via "publications of the
society" in http://www.msa.microscopy.com .

Ron Anderson, Editor
Email: microtoday-at-attglobal.net
________________________________________________

MICROSCOPY TODAY, May/June 2002
__________________________
Index of Articles
__________________________

Food Under The Microscope
Stephen Carmichael, Mayo Clinic

Some Thoughts On Vibrations In EM Laboratories
Anthony J. Garratt-Reed, MIT

The Emergence of Aberration Correctors for Electron Lenses
John Silcox, Cornell University

Fast OIM
D. J. Dingley*, S. Wright and M. Nowell TSL
(a subsidiary of EDAX)

Fitting a Student Microscope with a Consumer Digital Camera
Theodore M. Clarke, Metallurgical F. A. Consultant

Microscopy in the Real World: A Manufacturer's Perspective
Michael M. Kersker, JEOL USA, Inc.

All That Glitters is not Gold: Approaches to Labeling for TEM
R.M. Albrecht and D.A. Meyer, University of Wisconsin

Low Voltage Scanning Electron Microscopy and Jack Ramsey's principle
Oliver C. Wells, IBM Research Division

More on the Calibration of TEMs
J. P. McCaffrey, N.R.C. and R. Beanland,
Bookham Technology PLC

Lateral Resolution in Scanning Force Microscopy
Brian A. Todd and Steven J. Eppell
Case Western Reserve University

Utilizing Original TEM Negatives and Micrographs For Teaching in the
Digital Domain
Jos� A. Mascorro, Tulane University

Plunge-Freezing into Slush Nitrogen
Philip Oshel, University of Wisconsin

Downloadable Photoshop Convolution Plug-In
John Russ, North Carolina State University

A Home-made Antifade Medium for Fluorescent Dyes
Tim Plummer, Mayo Clinic

From daemon Mon May 6 18:32:13 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for all of your replies!

A few people suggested checking the water cooling system - we had to
replace the chiller last year, and we're replacing the water lines now at
the same time as we replace the vacuum lines in this overhaul - there are a
couple of very slow water leaks and some of the old rubber water lines are
very stiff. Our water tends to be on the cool side rather than too hot -
never more than 20C, though we did have a period of about a week or two
after the new chiller went in when the water got up to 24-25C. And all the
EMs were on tap water for about 6 months while we waited for the new
chiller....... But the oil problem started long before this.

The amazing thing about this TEM is that the image quality is pretty good.
However, some oil (or something) has definitely got into the column because
during an EM maintenance training session last year, we took the top off
the column and cleaned the upper chamber and gun - the inside of the
chamber was brown! However, since this was its first clean in at least 12
years, perhaps this isn't surprising. The TEM itself is 25 years old (so
the diff pump oil is also 25 years old) and has been in this building for
12 years. It may be that the move to this building caused a minor leak
somewhere in the system.

The guys have just started taking the diff pumps out, and we can see oil
leaking out of the solenoids on the vacuum system at the back of the
column..... We're all going to have fun, I can see!!!

Once it's clean and put back together, we'll follow Allen Sampson's
suggestion re. checking the cross-over vacuum setting.

cheers,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au

From daemon Mon May 6 23:07:56 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear friends,

I met a problem about the AFM images. As you know, although there is
no real meaning of 3-D image in AFM, it is a good format to show the
AFM result.
But the thing is: How to make two images have the same Z value?

My AFM system is the ThermoMicroscope. The software I use is Proscan.
Could somebody help me to find out how to get the same Z value for
different images out of my system? Or could you introduce another
reasonable software for me?

Looking forward for your help!

Yours, Xianglin Li

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411

From daemon Tue May 7 00:26:42 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How often do you pump down the camera?

As far as the PVC tubing, I have rarely seen cases where the proper
installation of roughing pump tubing of any kind induces a significant
vibration problem. Far more common are the building vibrations induced
through the structural members of the EM. However, I did qualify that as a
proper installation of the tubing. Case in point - one manufacturer's
service rep who replaced the tubing from the optics table to the vibration
isolator (the lead or concrete filled block usually used to dampen
vibrations). He added around 8 feet of extra tubing that he left coiled
on the floor claiming that it was known that that length of tubing was
required to reduce vibrations. Needless to say, when the excess was
removed the customer was greatly relieved that his FESEM actually could
image above 20KX.

Old DP oil can be a problem and the original poster has admitted to an
inordinate amount of time between changes. I assumed changes around 5
years and am culpable once again for the assumption that the service
organizations are doing their business properly and an apparently aware
operator would be apprised of the timing of good maintenance. Mea culpa.

Santovac 5 another one of my favorites. Anyone who knows me knows that I
am reluctant to endorse any particular product or service, but you've hit
two of my favorites in one posting and I can't help it.

You also bring up another pet peeve of mine - improperly placed foreline
traps. A foreline trap is a device that is inserted between a mechanical
pump and the instrument that is designed to condense oil vapors
backstreaming from the mechanical pump. Ideally, they should be placed at
least a few inches from the inlet of the pump, to provide thermal isolation
from the pump. A trap at room temperature will do a better job than one at
an elevated temperature caused by close contact with a constantly operated
pump.

The trap should be placed vertically so that the condensed oils can be
drawn back into the pump by gravity. Nothing worse than being called in to
fix an instrument that won't pump down only to find the foreline trap
laying horizontal on the floor and it and the vacuum hose flooded with
collected oil.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com

On Monday, May 06, 2002 2:42 PM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
wrote:
} ------------------------------------------------------------------------
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}
}
}
} } Date: Mon, 06 May 2002 14:39:09 -0700
} } To: "ars-at-sem.com" {ars-at-sem.com}
} } From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} } Subject: RE: cleaning vacuum system
} }
} } The only things you have to keep in mind: PVC tubes are less flexible
and
} } may 'transfer' vibration from rotary pump to the microscope's column.
For
} } this reason, I love to use PVC on vacuum evaporators nor the
microscopes.
} }
} } As for oil in the column - to me it looks like DP problem and water
} } waffler, which is situate over DP. The reason I think so, that usually
we
} } do not pump columns down by RP very often. It happening on my
microscope
} } a few times over 15 years, I believe. So, it mean, that RP do not touch
} } column frequently (except terrible downstream if it happening). I do
} } believe that 100C/CX DPs have water wafflers, check does water go
} } through. Another things: if DP oil is old (or overheated over some
period
} } of time) it may be deteriorated and boiling point may decreased (shorter
} } molecules), so some low-weight components may not condensate effectively
} } and will migrate into the column. I would suggest to check DP oil and
} } replace it on Santovak-5 like type (and forget for 5+ years). If you
} } decided to replace DP oil, I would suggest, you install oil traps on RPs
} } and new tubings as well to protect fresh oil (you never know...). Good
} } luck! Sergey
} }
} } At 05:04 AM 5/6/02 -0700, you wrote:
}
} } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
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}
} } -----------------------------------------------------------------------.
} } }
} } }
} } } Yes, a changeover to the clear PVC hosing is appropriate - I love the
} } } stuff. The problem you are experiencing is probably more a consequence
of
} } } the calibration of the vacuum system than anything else.
} } }
} } } Anyone who has been here for awhile has probably seen postings from me
on
} } } the vacuum system calibrations of EMs regarding the cross-over points
of
} } } diffusion pumped systems. Too often, design engineered or field
} } } engineering solutions result in a cross-over vacuum level from roughing
to
} } } diffusion pumps at too high a pressure level. When a diffusion pump is
} } } opened to a pressure level too high, it can stall which results in the
} } } breakdown of the normal laminar flow of oil vapor to a chaotic flow
which
} } } results in the influx of oil vapor into the sample chamber.
} } }
} } } Your first step should be to determine the actual chamber pressure at
which
} } } the cross-over from roughing to diffusion pump occurs. At the least,
you
} } } can get a qualitative determination from the action of the chamber
vacuum
} } } when the diffusion pump kicks in. At that point, the vacuum level
should
} } } start a rapid increase to the ultimate vacuum. If, instead, the vacuum
} } } level declines or holds steady for a few seconds, the cross-over point
is
} } } too high and the diffusion pump is stalling. Cross-over should
normally
} } } occur at 70 - 100 microns, most diffusion pumps will react well in this
} } } area. I generally tend to set the cross-over at 70 microns - it will
take
} } } longer for the roughing time but when the diffusion pump kicks in it
will
} } } rapidly pull down to the ultimate vacuum and the sample chamber will
remain
} } } cleaner.
} } }
} } } Field engineers don't normally carry independant vacuum measuring gear.
} } } Instead, they depend on known timing characteristics of known good
} } } systems. If your system doesn't match the characteristics expected,
the
} } } calibration will be off. The only offset to this is the use of a leak
back
} } } test, where the actual leak rate of the system can be tested. If the
leak
} } } back can be verified as within the manufacturer's specs, then the
} } } calibration can be applied and expected to achieve specs.
} } }
} } } If, however, there is no absolute measurement made of either actual
vacuum
} } } levels or actual leak rates, then no assumptions can possibly be made
of
} } } the vacuum levels achieved. Turbo-pumping systems actually make this
} } } determination of vacuum levels more problematic as they are seldom
capable
} } } of closing a valve on the sample chamber to allow the determination of
its
} } } leak rate, making the use of accurate vacuum level measuring equipment
} } } neccessary to determining the the calibration of system levels.
} } }
} } }
} } }
} } } Allen R. Sampson
} } } Advanced Research Systems
} } } 317 North 4th. Street
} } } St. Charles, Illinois 60174
} } }
} } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
} } }
} } }
} } } On Sunday, May 05, 2002 11:50 PM, Rosemary White
} } } [SMTP:rosemary.white-at-csiro.au] wrote:
} } } }
------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
-----------------------------------------------------------------------.
} } } }
} } } }
} } } } Dear all,
} } } }
} } } } We have a Jeol 100CX TEM that, I am told, has developed the
following
} } } } vacuum problem over the last 5-7 years. The problem, whatever its
cause,
} } } } means that the viewing port into the observation chamber has to be
} } } removed
} } } } once a month or so to clean off the buildup of oil on the inside. I
am
} } } } amazed that this was considered Ok for the microscope........ So,
my
} } } } proposal is to replace all the rubber vacuum lines, and clean out,
as
} } } best
} } } } as possible, the remaining components - metal hoses, etc. We're not
sure
} } } } of the cause of this problem, whether it's oil from the rotary pumps
or
} } } } from one of the diffusion pumps. Oh, and we need to clean, or at
least
} } } } check, the column as well, of course......
} } } }
} } } } My questions are:
} } } } Can we replace the original rubber tubing with wire-reinforced clear
PVC
} } } } tubing (at least then we'd see any oil buildup in future)? It's a
little
} } } } unclear how much vacuum the PVC tubing will take and this will be
} } } important
} } } } in the internals of the microscope.
} } } }
} } } } How can we identify the source of the problem? I'm proposing to put
} } } } foreline traps on both rotary pumps, but if a diff pump is the
problem,
} } } } this won't solve it. I'm also proposing to take out the diff pumps
and
} } } } check them, change the oil, etc. I've had a look through Will
Bigelow's
} } } } Vacuum Methods book (very useful!), and it's a bit like reading an
index
} } } of
} } } } diseases and their symptoms - you think you have a whole variety of
} } } them!!
} } } } - I can see several incidents and problems that could have affected
a
} } } } number of the vacuum components.
} } } }
} } } }
} } } } Thanks for any and all advice!
} } } } cheers,
} } } } Rosemary
} } } }
} } } } Rosemary White
} } } } Microscopy Centre
} } } } CSIRO Plant Industry
} } } } GPO Box 1600
} } } } Canberra, ACT 2601
} } } } Australia
} } } }
} } } } 61- 2 6246 5475 or
} } } } 61- 0402 835 973
} } } } rosemary.white-at-csiro.au
} } } }
} } } }
} } } }
} } } }
} } } }
}
}
}
}

From daemon Tue May 7 00:40:39 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Rosemary, you've got a lot of 'xplaining to do.

Has this instrument had any service at all in all those years? These
instruments can be remarkable tolerant, especially if the proper supplies
are used such as a DP oil that will form a non-conductive film. However,
you have two choices - either make column cleanliness a regular affair
where small amounts of contamination are removed at regular intervals, or
make column cleanliness a rare but substantial affair where it is left
alone until a problem develops and a major cleaning is required.

I have some sympathy for the task you now have, but take solace in the fact
that you brought it upon yourself. What you have is one of the most
sensitive and sophisticated scientific instruments ever made. Treat it in
the future as the fine instrument it is and you will have many more years
of happy service from it.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com

On Monday, May 06, 2002 4:29 PM, Rosemary White
[SMTP:rosemary.white-at-csiro.au] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Thanks for all of your replies!
}
} A few people suggested checking the water cooling system - we had to
} replace the chiller last year, and we're replacing the water lines now at
} the same time as we replace the vacuum lines in this overhaul - there are
a
} couple of very slow water leaks and some of the old rubber water lines
are
} very stiff. Our water tends to be on the cool side rather than too hot -
} never more than 20C, though we did have a period of about a week or two
} after the new chiller went in when the water got up to 24-25C. And all
the
} EMs were on tap water for about 6 months while we waited for the new
} chiller....... But the oil problem started long before this.
}
} The amazing thing about this TEM is that the image quality is pretty
good.
} However, some oil (or something) has definitely got into the column
because
} during an EM maintenance training session last year, we took the top off
} the column and cleaned the upper chamber and gun - the inside of the
} chamber was brown! However, since this was its first clean in at least
12
} years, perhaps this isn't surprising. The TEM itself is 25 years old (so
} the diff pump oil is also 25 years old) and has been in this building for
} 12 years. It may be that the move to this building caused a minor leak
} somewhere in the system.
}
} The guys have just started taking the diff pumps out, and we can see oil
} leaking out of the solenoids on the vacuum system at the back of the
} column..... We're all going to have fun, I can see!!!
}
} Once it's clean and put back together, we'll follow Allen Sampson's
} suggestion re. checking the cross-over vacuum setting.
}
} cheers,
} Rosemary
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} 61- 2 6246 5475 or
} 61- 0402 835 973
} rosemary.white-at-csiro.au
}
}
}
}
}

From daemon Tue May 7 01:47:20 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Allen,

Yes, well this is the state the instrument was in when I arrived here 2
years ago, but was told "it's OK, it works fine, don't worry about the oil
on the viewing window". As the new kid on the block and with no
experience in EM maintenance, I found it hard to argue for this full
overhaul. And yes, you could get OK images from it, but I still worried
about the oil, so finally we are doing something about it.

The diff pumps are out now, and if any of you have looked at Fig. 5.15 in
Wilbur Bigelow's Vacuum Methods book you'll have an idea what their innards
looked like. The top pump had a little oil left in it, with big chunks of
black goop in - and it looks like some of this junk and oil has gone
further up the vacuum system. The hot parts had brown to black carbonised
oil burnt firmly onto the surface. The innards of the bottom diff pump
were coated in what looked like blackstrap molasses - what's left of the
original oil, I guess. There was less carbonisation than in the top pump.

So, we're in for a long session, by the looks.......

Thanks again to everyone for suggestions and comments.

cheers,
Rosemary

} Rosemary, you've got a lot of 'xplaining to do.
}
} Has this instrument had any service at all in all those years? These
} instruments can be remarkable tolerant, especially if the proper supplies
} are used such as a DP oil that will form a non-conductive film. However,
} you have two choices - either make column cleanliness a regular affair
} where small amounts of contamination are removed at regular intervals, or
} make column cleanliness a rare but substantial affair where it is left
} alone until a problem develops and a major cleaning is required.
}
} I have some sympathy for the task you now have, but take solace in the fact
} that you brought it upon yourself. What you have is one of the most
} sensitive and sophisticated scientific instruments ever made. Treat it in
} the future as the fine instrument it is and you will have many more years
} of happy service from it.
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au

From daemon Tue May 7 02:46:31 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Funny stuff, DP oil. Over time and use, it seems to both crack, break down
into lower molecular weight products, and polymerize into larger molecular
weight fractions. The polymerization generally occurs on the metallic
surfaces of the pump and can only be removed by vigorous abrasive
techniques (metal polish and a lot of elbow grease or sandblasting with
glass beads - I haven't tried starch or CO2, but they may work). The gooey
mess can normally be removed with a good detergent (Dawn dishwashing soap)
or tri-chlor (not generally available anymore in the US). Mechanical
buffing wheels can be helpful but be aware that some pump parts may be made
of aluminum and will suffer material thinning if such means are used.

Sorry you inherited this mess. But it is a good guide to future
maintenance requirements.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com

On Monday, May 06, 2002 11:43 PM, Rosemary White
[SMTP:rosemary.white-at-csiro.au] wrote:
} Dear Allen,
}
} Yes, well this is the state the instrument was in when I arrived here 2
} years ago, but was told "it's OK, it works fine, don't worry about the
oil
} on the viewing window". As the new kid on the block and with no
} experience in EM maintenance, I found it hard to argue for this full
} overhaul. And yes, you could get OK images from it, but I still worried
} about the oil, so finally we are doing something about it.
}
} The diff pumps are out now, and if any of you have looked at Fig. 5.15 in
} Wilbur Bigelow's Vacuum Methods book you'll have an idea what their
innards
} looked like. The top pump had a little oil left in it, with big chunks
of
} black goop in - and it looks like some of this junk and oil has gone
} further up the vacuum system. The hot parts had brown to black
carbonised
} oil burnt firmly onto the surface. The innards of the bottom diff pump
} were coated in what looked like blackstrap molasses - what's left of the
} original oil, I guess. There was less carbonisation than in the top
pump.
}
} So, we're in for a long session, by the looks.......
}
} Thanks again to everyone for suggestions and comments.
}
} cheers,
} Rosemary
}
} } Rosemary, you've got a lot of 'xplaining to do.
} }
} } Has this instrument had any service at all in all those years? These
} } instruments can be remarkable tolerant, especially if the proper
supplies
} } are used such as a DP oil that will form a non-conductive film.
However,
} } you have two choices - either make column cleanliness a regular affair
} } where small amounts of contamination are removed at regular intervals,
or
} } make column cleanliness a rare but substantial affair where it is left
} } alone until a problem develops and a major cleaning is required.
} }
} } I have some sympathy for the task you now have, but take solace in the
fact
} } that you brought it upon yourself. What you have is one of the most
} } sensitive and sophisticated scientific instruments ever made. Treat it
in
} } the future as the fine instrument it is and you will have many more
years
} } of happy service from it.
} }
} } Allen R. Sampson
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} }
} } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com
}
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} 61- 2 6246 5475 or
} 61- 0402 835 973
} rosemary.white-at-csiro.au
}
}
}
}

From daemon Tue May 7 02:50:20 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Unsubscribe

--
Dr Adam Papworth,
Senior Experimental Officer,
Department of Engineering,
The University of Liverpool,
Liverpool,
L69 3GH, UK.

Phone
(Work) 0151 794 4672
(Mobile) 0151 794 7587
07970 24 7587
(Home) 0151 283 8596
(FAX) 0151 794 4675
e-mail ajp5-at-liv.ac.uk

From daemon Tue May 7 03:15:24 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Clontech is offering a new red fluorescent Protein hcRed.
Has someone made any experience using this protein.
Of special interest is the question of oligomerizatin or aggregation in
living cells since the older red fluorescent proteins have this
tendency.

greetings
Reinhard

--
--------------------------------------------------------------------
Dr. Reinhard Windoffer phone ++49-(0)6131 39 23720
Universitaet Mainz fax ++49-(0)6131 39 23719
Anatomisches Institut internet windoff-at-mail.uni-mainz.de
Becherweg 13 www.uni-mainz.de/~windoff
55099 Mainz
--------------------------------------------------------------------

From daemon Tue May 7 07:38:49 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Xianglin;

I am, and I would imagine others, are a bit confused as to what you are
using the AFM for? It sounds as if you are doing a thermal map over a
surface and not z-height measurements. If you are doing thermal
measurements are you attempting to combine them with z-height on a surface
as overlapping images?

Please elaborate and I'm sure someone can help.

Regards,
Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Xianglin Li [mailto:Xianglin_Li-at-student.uml.edu]
Sent: Tuesday, May 07, 2002 12:00 AM
To: Microscopy-at-sparc5.microscopy.com

Dear friends,

I met a problem about the AFM images. As you know, although there is
no real meaning of 3-D image in AFM, it is a good format to show the
AFM result.
But the thing is: How to make two images have the same Z value?

My AFM system is the ThermoMicroscope. The software I use is Proscan.
Could somebody help me to find out how to get the same Z value for
different images out of my system? Or could you introduce another
reasonable software for me?

Looking forward for your help!

Yours, Xianglin Li

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411

From daemon Tue May 7 08:29:48 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike,
I had a slight memory lapse regarding Audi so I spent 5 minutes to refresh
my memory. Here is a quote from The Center for Auto Safety:
"With nearly 1800 reported unintended acceleration incidents in the 1980s,
Audi became synonymous with the term "sudden acceleration." Despite several
deaths, dozens of injuries, five related recalls and a Swedish defense
agency study showing cruise control malfunctions could cause sudden
acceleration, Audi claimed that "pedal misapplication" caused the incidents.
Audi lost 80% of its market share because it chose to blame its customers
for the problem."
http://www.autosafety.org/autodefects/AUDI.htm

If memory serves me correctly one of the accidents actually occurred in a
New Jersey emissions inspection station! Ouch that's likely to put a dent in
your corporate image. Seems to me the free market did its job. As Sergey
Ryazantsev mentioned in his reply, it is unlikely that a single negative
posting will damage a credible companies reputation. It is usually a very
simple matter to verify and correct misinformation posted to this site.
That being said, I further agree with Sergey that the list has rules
governing participation that should be observed and enforced.

} John A. Robson
} _____________________________________________________
}
} Boehringer Ingelheim Pharmaceuticals, Inc.
} Research and Development
} 900 Ridgebury Road / P. O. Box 368
} Ridgefield, CT 06877-0368
}
} phone: 203.798.5640
} fax: 203.798.5698
} email: jrobson-at-rdg.boehringer-ingelheim.com
}
}
}
}
} -----Original Message-----
} From: Mike Bode [SMTP:mb-at-Soft-Imaging.com]
} Sent: Friday, May 03, 2002 3:05 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE:RONTEC USA Inc., Correction
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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}
}
} Unfortunately that is the case. Someone posts damaging material on this
} (or
} any other) list, it get's sent around to some people a few times, and
} suddenly it is "the official opinion of the microscopy specialists". I am
} sure that can do substantial damage.
}
} And it's not limited to the web or list servers. Audi (the German car
} manufacturer) had a good business going in the US until someone decided to
} start the engine and put it in forward (or backwards, I don't remember)
} and
} run the car into a wall. There was a flaw, that allowed users to put the
} car
} in gear without stepping on the brakes. Although that definitely was a
} flaw,
} it was blown out of proportion and almost killed the Audi business in the
} US. It took them 10 years to get their reputation back, even though I
} would
} not consider this as dangerous as the tire problem that Firestone/Ford was
} experiencing last year.
}
} Bottom line: Everybody should be careful and check their sources when
} posting potentially dmaging material on the listserver.
}
} But that's just my 2 cents as a vendor.
}
} mike
}
} } } } } } } } } } } WE HAVE MOVED { { { { { { { { {
} please make a note of the new address below
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Friday, May 03, 2002 6:40 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: RONTEC USA Inc., Correction
}
}
} ------------------------------------------------------------------------
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}
}
} It's strange to see that one anonymous message could make so much damage
} to
} the company. It seems to me that reputation of the company depends more
} on
} the quality of the products/service etc nor on the one anonymous
} opinion. From another hand, I think, this ListServer mostly is a place
} for
} 'customers' to exchange not only scientific ideas but to help each other
} to
} survive in this 'capitalistic' world. Information about wrongdoing may
} help others do not make similar mistake or spend money on bad quality
} product/service. This is sort of our 'protection' against bad quality
} service/products providers. Again, single decision even published here
} could not damage the reputation of the good company with long well
} established record of the service to EM community. I know, there are
} places on the Internet where you could leave your opinion about some
} particular business/company - it's perfectly legal and many institutions
} used that lists for decision making. This discussion is more about ethics
}
} - we all agree that we have rights openly speak here and others have the
} rights to know who is speaking.
}
} Sergey spoke.
}
} At 02:15 AM 5/3/02, you wrote:
} } ------------------------------------------------------------------------
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} } {http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} http://www.m
} sa
} .microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } To the Microscopy & Microanalysis community
} }
} } Ref.: Anonymous Message posted April 30,
} } Subj: R�NTEC USA Closes Down Operation
} }
} } Ladies and Gentlemen:
} }
} } Without any doubt, the Microscopy ListServer is a great facility for the
} } Microscopy&Microanalysis community to distribute and exchange useful
} } information in a very efficient way and everyone involved in initiation
} } and maintenance of this forum highly deserves our community's credit.
} }
} } However, it appears that at the same time this platform is a quite
} } powerful tool for anyone with hostile and bad enough intents to defame
} and
} } defile disliked entities. This is what we had to learn when the
} } unwarranted message regarding an alleged close down of R�NTEC USA, Inc.
} } was spread. It also appears that there is no way to avoid such unfounded,
}
} } false and misleading information to be posted at the ListServer other
} than
} } the commitment of all users to the "General Ground Rules on using the
} } Microscopy Listserver/Mailreflector" (see msa website), clearly a code of
}
} } honor. Whatever individual or organization posted this message in their
} } malicious attempt of damaging our company and good reputation violated
} all
} } those general ground rules. May everyone draw his/her own conclusions
} } concerning the nature of the information and the integrity of the sender.
} }
} } We may be allowed to correct the above-mentioned statements about R�NTEC
} } USA, Inc. as follows: 1. There are no plans whatsoever to cease the
} } business of R�NTEC USA, Inc.
} } 2. The R�NTEC Group of companies - like so many others - has not
} } remained unaffected by the overall downturn we saw in the economy during
} } the year 2001. The situation within our group forced us to implement a
} } restructuring program in order to reduce operational costs (as was the
} } case throughout the industry - we believe we are in good company here).
} } This program was comprising all members of our group, including RONTEC
} } USA. The goal was not to cease business, but exactly the opposite - to
} } ensure its continuation, in the best interest of our customers.
} } 3. Our joint efforts have been successful. The fact is R�NTEC USA,
} } Inc. has had its best sales year since the company's founding in 1999
} } nearly doubling its revenue over the previous year. Why, after having
} } worked very hard indeed to get things going and after having eventually
} } accomplished that stage, should we consider to discontinue the business?
} } Apparently there exists someone or some company who is attempting to
} } damage our reputation. Be assured that R�NTEC USA is in a better position
}
} } than ever before and will continue to grow and continue to support its
} } customers.
} } 4. We have never provided misleading information on the equipment we
}
} } are offering. How long would we survive in this business if we were to
} } make promises we can't keep? Due to our companies policy of verifying any
}
} } information before disclosing it to the public we earned ourselves a
} } reputation as a reliable business partner.5. R�NTEC USA, Inc. does
} } have an SEM for sale, however this has absolutely no bearing on the
} } viability of the company.
} }
} }
} } Whoever has any questions or the need for more information on this
} subject
} } may please contact us directly, by phone or otherwise, in the US or at
} our
} } German headquarters. We at R�NTEC take great pride in our products and
} } services and we have nothing to hide - in contrast to the sender of that
} } unfounded message, who chose not to disclose a name.
} } In the future may our community be spared such unwarranted assaults.
} }
} }
} } Thomas Schuelein Paul Smith
} } President & CEO President & CEO
} } R�NTEC Holding AG R�NTEC USA, Inc.
} }
} }
} } {/blockquote} {/x-html}
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}

From daemon Tue May 7 08:38:34 2002

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-----Ursprungliche Nachricht-----
Von: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Gesendet: Tuesday, May 07, 2002 3:03 PM
An: NewSub-at-sparc5.microscopy.com
Betreff: Welcome to the Microscopy Listserver

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*********************************************
This Email contains Important Information about
the Microscopy Listserver. Please read it all then
SAVE a copy for future reference. - Nestor
****************************************
To: NewSub-at-MSA.Microscopy.Com
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}

Hi Peter:

I am very sorry to make you and other friends confused with my
question.

Actually, I am just doing the regular AFM, that is, using AFM to get
the topography information. It is the z height what you mentioned.

I want to put some images in my presentation. While, as you know, 3-D
works better than 2-D if you just want to "show" them. The problem is:
different 3-D images have their own Z range. If I want to compare
them, I'd better use the same Z range for every image, so that when
people look at them, they will have some idea immediately.

This is what I want to do: Keep same Z range for different sample's
image. But I failed when I tried to reach it by only changing the Z
magnification and offset.

But for lots of papers, which have AFM images included, their images
have the constant Z range for all the images. So I just wonder whether
some of you did this for your own images, and whether you could help
me out about this.

Thank you very much!

Sincerely, Xianglin Li

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411

Center for Advanced Material
Department of Chemical Engineering
University of Massachusetts, Lowell
Xianglin_Li-at-student.uml.edu
Tel: 978-934-3411

----- Original Message -----
} From: Peter Tomic {PTomic-at-Anadigics.com}

I am working with 27nm cobalt particles that are weakly ferromagnetic (MS =
95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
am thinking of also using a JEOL 840 SEM to analyze the aggregated
structures formed once the particles are dried onto a substrate. The
substrates I am using are carbon grids for the TEM and I am planning to use
silicon wafers for SEM. I am unsure of the adhesion of the particles to the
substrates, although suspect adhesion to the carbon grid will be better than
to the silicon (the particles are coated with an organic surfactant). I
would prefer to obtain the SEM images without coating the sample. What is
the possibility that these particles will be picked up by the lenses in the
EM? Are there other considerations I should keep in mind while planning
these experiments?

Thank you,

Steve Tripp

Dept. of Chemistry
Purdue University
West Lafayette, IN 47907

From daemon Tue May 7 15:27:50 2002

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In the early days of materials ultramicrotomy at my laboratory, we plastered
more than a few sections of steel onto the polepiece of our EM400T as soon
as the polepiece was activated in going from low mag to regular mode. One
minute they were there, then---poof! However in an old warhorse like that,
there was no noticeable effect on the TEM's performance. (Our
superconscientious tech did glare rather strongly just at the thought of
such an indiscretion, mind).

So I wouldn't worry too much. We got tired of losing metal thin sections
that were dried onto grids in any event, and found that collecting them on
the film side of a coated grid resulted in more than enough 'stick' to hold
them there. Of course, these sections had pretty huge aspect ratios
(thickness/width of several hundred) compared to a particle, but I think
your low mass should help out there.

Good luck.

Tom

Dr. Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada K1A 0G1

ph. 613-992-2310
FAX 613-992-8735

email: malis-at-nrcan.gc.ca
(currently on assignment as Science Advisor to DG/MTB, can be reached at
613-995-7358, same email)

} ----------
} From: Steven L. Tripp
} Sent: Tuesday, May 07, 2002 1:14 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: EM of magnetic particles
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am working with 27nm cobalt particles that are weakly ferromagnetic (MS
} =
} 95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
} and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
} am thinking of also using a JEOL 840 SEM to analyze the aggregated
} structures formed once the particles are dried onto a substrate. The
} substrates I am using are carbon grids for the TEM and I am planning to
} use
} silicon wafers for SEM. I am unsure of the adhesion of the particles to
} the
} substrates, although suspect adhesion to the carbon grid will be better
} than
} to the silicon (the particles are coated with an organic surfactant). I
} would prefer to obtain the SEM images without coating the sample. What is
} the possibility that these particles will be picked up by the lenses in
} the
} EM? Are there other considerations I should keep in mind while planning
} these experiments?
}
}
} Thank you,
}
} Steve Tripp
}
} Dept. of Chemistry
} Purdue University
} West Lafayette, IN 47907
}
}
}

From daemon Tue May 7 16:15:13 2002

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Yuck I've scrubbed DP's like that before, Sorry You inherited this mess but
they will be amazed at how good it works when you are done. yearly PM's are
well worth never having to do this again I'm sure you will agree by the time
you get it all put back together. Good Luck.

Kathy Napolitano

-----Original Message-----
} From: Rosemary White [mailto:rosemary.white-at-csiro.au]
Sent: Monday, May 06, 2002 11:43 PM
To: ars-at-sem.com
Cc: Microscopy-at-sparc5.microscopy.com

Dear Allen,

Yes, well this is the state the instrument was in when I arrived here 2
years ago, but was told "it's OK, it works fine, don't worry about the oil
on the viewing window". As the new kid on the block and with no
experience in EM maintenance, I found it hard to argue for this full
overhaul. And yes, you could get OK images from it, but I still worried
about the oil, so finally we are doing something about it.

The diff pumps are out now, and if any of you have looked at Fig. 5.15 in
Wilbur Bigelow's Vacuum Methods book you'll have an idea what their innards
looked like. The top pump had a little oil left in it, with big chunks of
black goop in - and it looks like some of this junk and oil has gone
further up the vacuum system. The hot parts had brown to black carbonised
oil burnt firmly onto the surface. The innards of the bottom diff pump
were coated in what looked like blackstrap molasses - what's left of the
original oil, I guess. There was less carbonisation than in the top pump.

So, we're in for a long session, by the looks.......

Thanks again to everyone for suggestions and comments.

cheers,
Rosemary

} Rosemary, you've got a lot of 'xplaining to do.
}
} Has this instrument had any service at all in all those years? These
} instruments can be remarkable tolerant, especially if the proper supplies
} are used such as a DP oil that will form a non-conductive film. However,
} you have two choices - either make column cleanliness a regular affair
} where small amounts of contamination are removed at regular intervals, or
} make column cleanliness a rare but substantial affair where it is left
} alone until a problem develops and a major cleaning is required.
}
} I have some sympathy for the task you now have, but take solace in the fact
} that you brought it upon yourself. What you have is one of the most
} sensitive and sophisticated scientific instruments ever made. Treat it in
} the future as the fine instrument it is and you will have many more years
} of happy service from it.
}
} Allen R. Sampson
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
}
} phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

61- 2 6246 5475 or
61- 0402 835 973
rosemary.white-at-csiro.au

From daemon Tue May 7 19:46:46 2002

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We are looking for a simple sub-stage illuminator for an old Olympus POS
vertical monocular microscope. Any suggestions would be appreciated.

Don Marshall

Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

781-275-4695 (Phone)
781-271-0252 (FAX)

dmrelion-at-aol.com

http://www.excitingelectrons.com

From daemon Tue May 7 21:25:58 2002

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Dear Sir,

We are glad to send the introductory to you in order to enter into
business relation with you.
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E-mail: chineseoilpress-at-hotmail.com

From daemon Wed May 8 02:32:28 2002

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Message-ID: {3CD8D25D.A48BB9E9-at-ru.ac.za}

Hi.
We're investigating various alternatives for the polishing of rocks
of various kinds. One of the suggestions is to go the Struers' MD-system
route, but as you can imagine this is the expensive option. Is there
anyone out there using this and how is it working for you? My particular
concerns are lif time of the discs and enbedding of foreign particles
(mineral frags that may chip off) into the polymer surface rendering
these unusable.
Thanks,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (try your luck!)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA

From daemon Wed May 8 02:51:22 2002

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Hi Steven,

Common sense is (as always) most important. However, as
most common sense comes with hindsight I'll give a few
thoughts.

I'll assume that you are not thinking of applying fields
in-situ or looking for magnetic contrast but if you are
there are other considerations.

Don't forget that unless you have a low field pole piece
the specimen will be sitting in a field when you analyse
it. In the TEM this will be a high field and may negate
your ex-situ experiment.

It seems sensible to use a long working distance in the
SEM to reduce the field but I have no idea what a SEM field
profile looks like.

In the TEM switch off the objective lens before inserting
the specimen and slowly increase the strength to avoid
sudden field changes.

Even if using a low field pole piece don't forget the
return path of the lens field is through the outer column
and there will be a high field as the specimen is inserted
through the airlock.

Keep your beam current low to prevent the possibility of
particle charging adding to the force to free the particles
from the substrate.

Don't forget to tap the specimens to free any loose
particles before inserting them into the microscope.

Check particle dispersion under an optical microscope
first, large agglomerations are more likely to give
problems. (You may not see the particles but you will see
large agglomerations of particles.)

Good luck,
Ron

On Tue, 7 May 2002 12:14:14 -0500 "Steven L. Tripp"
{tripp-at-purdue.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am working with 27nm cobalt particles that are weakly ferromagnetic (MS =
} 95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
} and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
} am thinking of also using a JEOL 840 SEM to analyze the aggregated
} structures formed once the particles are dried onto a substrate. The
} substrates I am using are carbon grids for the TEM and I am planning to use
} silicon wafers for SEM. I am unsure of the adhesion of the particles to the
} substrates, although suspect adhesion to the carbon grid will be better than
} to the silicon (the particles are coated with an organic surfactant). I
} would prefer to obtain the SEM images without coating the sample. What is
} the possibility that these particles will be picked up by the lenses in the
} EM? Are there other considerations I should keep in mind while planning
} these experiments?
}
}
} Thank you,
}
} Steve Tripp
}
} Dept. of Chemistry
} Purdue University
} West Lafayette, IN 47907
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk

From daemon Wed May 8 04:59:54 2002

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Dear all,
What programs are people using these days for calculating the line direction
of dislocations and other linear features (and thus also habit planes of
planar features) from measurements made on conventional TEM diffraction
contrast images?

Several years ago I did this with Diffract (for Mac) as a PhD student, but
no error was included in the analysis, and Diffract was always prone to
unexpected crashes. Maybe this is done better in Desktop Microscopist (the
successor to Diffract) but I don't know myself.

Or maybe there's some other program to recommend?

I remember seeing a routine published by Fu-Rong Chen and Alex King for
Hexagonal Materials which calculated a best fit to available data. Did
anyone make such a thing into a program? I could probably do it with Excel,
but if somebody has already done it then it saves the work.

Well, I look forward to hearing from you.

Best wishes

Ian MacLaren
N.B. New address
FB Materialwissenschaft - FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162893
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/

From daemon Wed May 8 07:47:42 2002

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Steve;

Would there be a problem in elevating your samples above the Fermi
Temperature and thereby make them non-magnetic? Or would this change the
characteristics of your alloy? I am interested since I have had a similar
problem with an iron alloy but solved it by coating and imaging as you have
mentioned.

With respect to these particles on a Si wafer in SEM, you may try depositing
Si3n4 [silicon nitride] over the particles while on the wafer if you have
access to a deposition system such as a PECVD. This can be a very thin
film, just a few hundred angstroms, but it will lock these particles to the
surface and still allow imaging even if they are irregular surfaces.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Steven L. Tripp [mailto:tripp-at-purdue.edu]
Sent: Tuesday, May 07, 2002 1:14 PM
To: Microscopy-at-sparc5.microscopy.com

I am working with 27nm cobalt particles that are weakly ferromagnetic (MS =
95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
am thinking of also using a JEOL 840 SEM to analyze the aggregated
structures formed once the particles are dried onto a substrate. The
substrates I am using are carbon grids for the TEM and I am planning to use
silicon wafers for SEM. I am unsure of the adhesion of the particles to the
substrates, although suspect adhesion to the carbon grid will be better than
to the silicon (the particles are coated with an organic surfactant). I
would prefer to obtain the SEM images without coating the sample. What is
the possibility that these particles will be picked up by the lenses in the
EM? Are there other considerations I should keep in mind while planning
these experiments?

Thank you,

Steve Tripp

Dept. of Chemistry
Purdue University
West Lafayette, IN 47907

From daemon Wed May 8 08:57:27 2002

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can anyone tell me where i might purchase uranyl formate in the US?

From daemon Wed May 8 08:57:27 2002

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can anyone tell me where i might purchase uranyl formate in the US?

From daemon Wed May 8 09:41:17 2002

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Good morning to all.

I was wondering if the group had any wisdom to impart that could help me
with my particle analysis work. I'm trying to analyse stack effluent
collected on polycarbonate filters during an airborne survey. I'm using WDS
because the sample contain Pb and S. The particles range from less than one
to ~ 10 um. My problem is that most of the are charging and therefore the
beam is drifting off of them during the course of analysis. The samples
polycarbonate filtres are attached to Al stubs using double stick carbon and
heavily carbon coated while on a planetary rotating table. I'm using 15 kV
(I need to see Fe)and 10-20 nA current. I've had some success overscanning
the particles (i.e analysing with a raster that is the same size as the
particle) but some of the larger particles are multiphase and i would like
to use a smaller beam to determine their chemistry. Any tips or comments
from people with experience in this type of work would be gladly accepted.

Thanks

Glenn
Glenn Poirier
Microbeam Specialist

Mine and Mineral Science Laboratories (CANMET)
Rm. 213b, 555 Booth st.
Ottawa, On, K1A 0G1
TEL: (613) 947-9833
glpoirie-at-nrcan.gc.ca

From daemon Wed May 8 15:02:48 2002

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In the below first paragraph below I mentioned "Fermi Temperature" and
should have said "Curie Temp."

My appologies.

Peter

-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Wednesday, May 08, 2002 8:44 AM
To: 'Steven L. Tripp'; Microscopy-at-sparc5.microscopy.com

Steve;

Would there be a problem in elevating your samples above the Fermi
Temperature and thereby make them non-magnetic? Or would this change the
characteristics of your alloy? I am interested since I have had a similar
problem with an iron alloy but solved it by coating and imaging as you have
mentioned.

With respect to these particles on a Si wafer in SEM, you may try depositing
Si3n4 [silicon nitride] over the particles while on the wafer if you have
access to a deposition system such as a PECVD. This can be a very thin
film, just a few hundred angstroms, but it will lock these particles to the
surface and still allow imaging even if they are irregular surfaces.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Steven L. Tripp [mailto:tripp-at-purdue.edu]
Sent: Tuesday, May 07, 2002 1:14 PM
To: Microscopy-at-sparc5.microscopy.com

I am working with 27nm cobalt particles that are weakly ferromagnetic (MS =
95 emu/g, HC = 175 Oe) and am attempting some experiments employing static
and dynamic magnetic fields. I am currently using a Philips EM400 TEM and
am thinking of also using a JEOL 840 SEM to analyze the aggregated
structures formed once the particles are dried onto a substrate. The
substrates I am using are carbon grids for the TEM and I am planning to use
silicon wafers for SEM. I am unsure of the adhesion of the particles to the
substrates, although suspect adhesion to the carbon grid will be better than
to the silicon (the particles are coated with an organic surfactant). I
would prefer to obtain the SEM images without coating the sample. What is
the possibility that these particles will be picked up by the lenses in the
EM? Are there other considerations I should keep in mind while planning
these experiments?

Thank you,

Steve Tripp

Dept. of Chemistry
Purdue University
West Lafayette, IN 47907

From daemon Wed May 8 15:30:52 2002

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We sold uranyl formate for many years but we discontinued it in 1995 and
have been unable to find a new source.

John Arnott
--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
FAX: 1-802-660-8859
e-mail: sales-at-laddresearch.com

Quality Since 1955

John Hoffpauir wrote:
}
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} can anyone tell me where i might purchase uranyl formate in the US?

From daemon Wed May 8 17:15:26 2002

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

John Hoffpauir wrote:
==================================================
can anyone tell me where i might purchase uranyl formate in the US?
==================================================
Try URL
http://www.2spi.com/catalog/chem/stain.shtml

The product is in stock.

Chuck

Disclaimer: SPI Supplies is a supplier of uranyl formate.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================

From daemon Wed May 8 20:07:14 2002

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We need to replace a Nikon Condenser Lens (MBL12100-AL1) from a Nikon Phase
Contrast Unit (1.25 59842) being used on a Nikon Labophot Microscope.
This microscope is being used for the phase contrast analysis of air
samples for fiber concentration.

We have been in touch with Nikon and one of their suppliers but so far
without too much success. Does anyone know of any other sources for this
lens or a replacement phase contrast unit?

Thanks .

Stanley H. Gelles
Principal Scientist
CC Technologies
6141 Avery Road
Columbus, OH 43220
614-761-1214
FAX 614761-1633
sgelles-at-cctlabs.com

From daemon Wed May 8 20:07:20 2002

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (alm958-at-lulu.it.northwestern.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
May 8, 2002 at 17:58:01
---------------------------------------------------------------------------

Email: alm958-at-lulu.it.northwestern.edu
Name: Tony Meier

Organization: Northwestern University

Education: Graduate College

Location: Evanston, IL ,USA

Question: Why are we getting a clear and focused image with our
infinity corrected objective attached directly to our vidicon camera,
without using a tube lens? I realize that we are not getting
anywhere near the 10x mag of the objective but I don't understand why
we are getting an image at all since we are not forming a real image
on our cameras sensor.

Thanks

---------------------------------------------------------------------------

From daemon Wed May 8 23:05:23 2002

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I am looking to purchase a fluorescent microscope ( I am partial to Olympus
and Leica but am open to other options). It needs to have oil (100X) and
phase contrast for LM, DIC would be nice too, and all three filter sets,
DAPI, FITC, and TRITC. A used one in good condition would be acceptable.

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112

From daemon Thu May 9 00:52:52 2002

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I am interested in the current negative-scanner technology, but I would
like to find a non-contact scanner. EM emulsions are very soft and very
high resolution, so since these negatives represent the basis for long
term archiving, I wouldn't want to sacrifice microscopic information by
placing it on a surface of glass (or any surface) with potentially
damaging particulate on the surface. Even projection holders for
Negatives have no glass that contact the emulsion.
So what is the current negative scanner technology?? Non-contact..?

Jeff Day/Texas
wa5ekh-at-juno.com

From daemon Thu May 9 05:49:33 2002

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During several years I used Ralph Knifes to section Glycol Methacrylate
embedded material (2-3 micrometer) for LM.
Has anyone experience in sectioning with disposable blades?

Thanks for the courtesy
Bruno Dore

From daemon Thu May 9 10:20:50 2002

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Many of our customers are using Microtek Artixscan 2500 and 1100 scanners
as well as their mechanical twins, the Agfa Duoscan T2500 and Hi-D. Agfa
has discontinued the Duoscan line but the Microteks are still available.
The 2500 has higher optical resolution, the 1100 has a wider dynamic range.
These scanners hold films in a glassless drawer much like a negative carrier
in an enlarger. Most are using the 4x5 opening to hold the 3.25 x 4 EM
negs.

More information is available at http://www.microtekusa.com/as2500.html for
the 2500, and
http://www.microtekusa.com/as1100.html for the 1100. We also have a PDF
available.

George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com

} I am interested in the current negative-scanner
} technology, but I would like to find a non-contact
} scanner.

From daemon Thu May 9 11:07:04 2002

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I just wondered if anybody heard about LaB6 filament reconditioning and if
it's worth it.

Thanks,
Pavel
atcsem-at-earthlink.net

From daemon Thu May 9 11:56:40 2002

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Life Science Research Assistant (LSRAI) (HR #001178). Range: 2P1.

DESCRIPTION: Life Sciences Research Assistant is sought for day to day
managing of the Cell Sciences Imaging Facility (CSIF). The CSIF is a
service center that specializes in the use of high technology fluorescent
microscopes (confocal, deconvolution, ratio imaging) in biomedical
research. LSRA duties include: training users in operation of microscopes,
providing ongoing technical supervision, performing routine
maintenance/cleaning and arranging for service of microscopes and
computers. Additionally, LSRA will provide some administrative support
such as assisting in setting up user accounts, updating user databases and
monthly billing routines.

All of CSIF microscopes are interfaced with computers for data collection
and analysis. Basic knowledge of operating systems (Windows, Mac, Unix ),
graphics programs (PhotoShop, Illustrator) and general computer usage
(transfer of files between platforms, file format differences) is
essential. LSRA should understand optical properties of fluorescence.
Preference will be given to applicants who have both basic microscopy and
computer experience, but a willingness to learn is key. Qualifications: BS
degree required, as well as good communication skills, patience, and the
ability to work independently. Good problem solving skills are essential.
This is a great opportunity learn state-of-the-art technology and be
exposed to current biomedical research.

Salary range $37,100 - 41,000, depending upon experience. There is no
relocation assistance; position is available immediately. Please send
resume directly to:

Jon Mulholland, Director
Cell Sciences Imaging Facility
Beckman Center B050
Stanford University School of Medicine
Stanford, CA 94305

650-725-4951 (f)

From daemon Thu May 9 12:33:00 2002

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To all subscribers:

I am looking to obtain a pore size distribution and a porosity estimate of a
sample from two scanning electron micrographs taken at a 10� offset from one
another. Both files are tiff format. All of the software I was able to find
uses z-stacking and not an angular offset. Does anyone have any
recommendations on software for generating a 3-D image and obtaining the
information? Thank you for your time.

Dan

****************************************************************************
******************************************************************
Daniel D. Smolko, Ph.D.
Senior Research Scientist & BioChemical Engineer
Nanogen, Incorporated
10398 Pacific Center Court
San Diego, CA 92121

Tel.# (858) 410-4798
Fax.# (858) 410-4848
e-mail: dsmolko-at-nanogen.com

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and may contain information that is privileged, confidential and is exempt
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have received this communication in error, please notify us immediately by
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From daemon Thu May 9 13:14:18 2002

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I would like to have my name removed from this listserve.
Thank You.

From daemon Thu May 9 13:29:10 2002

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Hello Listers,

We have a visiting scientist interested in learning FISH techniques -- are
there any class(es)/workshops out there being offered in the near future
(ie. this summer or fall)?

TIA,

John

John W. Mattila
Iowa State University
Bessey Microscopy Facility
Room 1, Bessey Hall
Ames, IA 50011-1020

Ph: 515 294-3872
Fax: 515 294-1337

From daemon Thu May 9 14:44:42 2002

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Hi Malc,
We polish many different crystals and materials here.
We also build our own lapping and polishing equipment.
I thought I might be able to assist you in making a cost-effective
choice in selecting equipment if I were able to learn just exactly
what you need to do. We routinely achieve surfaces with as low
as 3 to 4 Angstrom RMS roughness here.
Let me know if I can be of help to you.

Cheers,
Michael Urbanik
} From the U.S.A., the keeper of the Flame of Freedom.
www.crystalguru.com

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

} Hi.
} We're investigating various alternatives for the polishing of rocks
} of various kinds. One of the suggestions is to go the Struers' MD-system
} route, but as you can imagine this is the expensive option. Is there
} anyone out there using this and how is it working for you? My particular
} concerns are lif time of the discs and enbedding of foreign particles
} (mineral frags that may chip off) into the polymer surface rendering
} these unusable.
} Thanks,
} Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (try your luck!)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA

From daemon Thu May 9 16:31:40 2002

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Dan
You may find the Soft Imaging System web site relevant. There is a
module for AnalySIS
that calculates quantitative elevation maps based on stereo pair
images

http://www.soft-imaging.de/eng/products/modules/stereo/ste_e.php

Chris

----- Original Message -----
} From: "Smolko, Dan" {DSmolko-at-Nanogen.com}
To: "'Microscopy-at-MSA.Microscopy.Com'"
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 09, 2002 6:21 PM

To all subscribers:

I am looking to obtain a pore size distribution and a porosity
estimate of a
sample from two scanning electron micrographs taken at a 10� offset
from one
another. Both files are tiff format. All of the software I was able
to find
uses z-stacking and not an angular offset. Does anyone have any
recommendations on software for generating a 3-D image and obtaining
the
information? Thank you for your time.

Dan

**********************************************************************
******
******************************************************************
Daniel D. Smolko, Ph.D.
Senior Research Scientist & BioChemical Engineer
Nanogen, Incorporated
10398 Pacific Center Court
San Diego, CA 92121

Tel.# (858) 410-4798
Fax.# (858) 410-4848
e-mail: dsmolko-at-nanogen.com

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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recipient(s) entity to which it is addressed. This message is
confidential
and may contain information that is privileged, confidential and is
exempt
from disclosure under applicable law. If you are not the intended
recipient(s), you may not review, copy, or distribute this message. If
you
have received this communication in error, please notify us
immediately by
e-mail, or telephone and delete the original message. Thank you.
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From daemon Thu May 9 17:44:39 2002

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} During several years I used Ralph Knifes to section Glycol Methacrylate
} embedded material (2-3 micrometer) for LM.
} Has anyone experience in sectioning with disposable blades?
}
} Thanks for the courtesy
} Bruno Dore

Bruno -

You can hand-break Ralph knives from glass microscope slides. They're
cheap, good, and disposable.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

From daemon Thu May 9 17:54:13 2002

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Dan,

what you are looking for is a stereo reconstruction program. There are
several available, we make on too. If you want more information, please
contact me offline at the address below.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: Smolko, Dan [mailto:DSmolko-at-Nanogen.com]
Sent: Thursday, May 09, 2002 11:21 AM
To: 'Microscopy-at-MSA.Microscopy.Com'

To all subscribers:

I am looking to obtain a pore size distribution and a porosity estimate of a
sample from two scanning electron micrographs taken at a 10� offset from one
another. Both files are tiff format. All of the software I was able to find
uses z-stacking and not an angular offset. Does anyone have any
recommendations on software for generating a 3-D image and obtaining the
information? Thank you for your time.

Dan

****************************************************************************
******************************************************************
Daniel D. Smolko, Ph.D.
Senior Research Scientist & BioChemical Engineer
Nanogen, Incorporated
10398 Pacific Center Court
San Diego, CA 92121

Tel.# (858) 410-4798
Fax.# (858) 410-4848
e-mail: dsmolko-at-nanogen.com

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|||||||||||||||||||||||||||||
This e-mail message is intended only for use by the individual, or
recipient(s) entity to which it is addressed. This message is confidential
and may contain information that is privileged, confidential and is exempt
from disclosure under applicable law. If you are not the intended
recipient(s), you may not review, copy, or distribute this message. If you
have received this communication in error, please notify us immediately by
e-mail, or telephone and delete the original message. Thank you.
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From daemon Thu May 9 21:03:48 2002

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in addition to the SIS stereo reconstruction module, there is a similar
routine that is part of Fovea Pro. See
http://ReindeerGraphics.com/foveapro2/surface.html

From daemon Fri May 10 08:30:22 2002

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unsubscribe

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 JAH
1020 Locust Street
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cell
Timothy.Schneider-at-Mail.TJU.edu

From daemon Fri May 10 08:30:38 2002

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anyone out there have any experience using the edwards vacumm evaporator to
visualize macromolecules?
john

From daemon Fri May 10 08:46:37 2002

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Dear All,

We have a Philips EM400T in our lab. It is old but still works well. However, I
noticed that illumination can be observed as soon as High Tension is on. As a
result, the saturation point is very low, only two or three steps of filament
current. My question is why there is a clear emmission current without any
filament current? Is this caused by a gun fault? What can we do about it?
Thank you very much in advance!

Yanling

Dr.Y.L.Chen
MicroStructural Studies Unit
School of Engineering
The University of Surrey
Guildford, Surrey
GU2 7XH, England

---------------------------------------------
This message was sent using UNIS MailSystem.

From daemon Fri May 10 09:10:57 2002

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} } I am interested in the current negative-scanner
} } technology, but I would like to find a non-contact
} } scanner.

If you have a robust budget, (12-18K$) you might check the Imacon
scanners, either the Flextite 848 or Precision III.

I have no commercial interest in these products.
--
Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice

From daemon Fri May 10 11:18:07 2002

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I am looking for individuals who are willing to review and write a
reviewed article of the following books for the MSA Journal.

1. "Electron Microscopy and Analysis 2001" (A materials related
book). Edited by M. Aindow and C.J. Kiely
2. "Luminescence Biotechnology Instruments and Applications" Edited
by Knox Van Dyke, Christopher Van Dyke and Karen Woodfork
3. " Photography with a Microscope" Fred Rost and Ron Oldfield
4. " Selected Papers on Optical Low-Coherence Reflectometry &
Tomography" Edited by Barry R. Masters
5. "Near-Infrared Technology in the Agricultural and Food
Industries" Edited by Phil Williams and Karl Norris

If you are interested in participating please send me the title of the
book you would like to review and it will be sent to you ASAP. Selection
will be make on a first come first serve basis.

Thank you!

JoAn Hudson, PhD.
Institute of Neuroscience
222 Huestis Hall
University of Oregon,
Eugene, OR 97403
�
Telephone: (541) 346 4508
FAX: (541) 346 4548

From daemon Fri May 10 12:50:36 2002

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Dear Colleague,

Please find enclosed deadlines related to TNT2002 conference to be held in Santiago de Compostela (Spain): September 9-13, 2002:

!!May 17, 2002: Extended "Graduate grant request" deadline!!
!!May 24, 2002: Extended Abstract submission deadline!!
June 03, 2002: Notification to the authors by e-mail of the accepted papers for oral presentation or poster / Notification of graduate travel awards
June 17, 2002: Registration deadline
July 01, 2002: Preliminary programme
July 15, 2002: Final programme & "Extended Abstracts" booklet printing
September 09, 2002: Manuscript submission deadline (to be published in "Nanotechnology" journal)

40 Grants for students are available: 20 for US and 20 for Europe - please check http://www.cmp-cientifica.com/cientifica/frameworks/generic/public_users/TNT02/TNT02_grants.html for more info.

If you are interested in presenting an oral contribution, please send me (mailto:antonio-at-cmp-cientifica.com) as soon as possible a tentative title talk and an extended abstract (2 pages max including figures) - only a few slots are still available. Therefore, the committee will select papers for oral presentation and we will notify authors shortly. We encourage participants to present posters during TNT2002.

If you need more information, please do not hesitate to contact me. If you accept this invitation, please respond by email as soon as possible.

Hoping to receive a positive answer to our invitation.

Antonio Correia

-----------------------------------------------------------------------------------
Dr. Antonio CORREIA - Coordinator of the IST Nanoelectronics Network (PHANTOMS)
CMP Cientifica S.L.
Phone: +34 91 6407187 Fax: +34 91 6407186
mailto:antonio-at-cmp-cientifica.com
WEB site: http://www.cmp-cientifica.com/
PHANTOMS WEB site: http://www.phantomsnet.com/
TNT2002: http://www.cmp-cientifica.com/TNT2002.html

------------------------------------------------------------------------------------
Please find enclosed confirmed TNT02 Keynote Speakers (09/05/2002):

1. Masakasu Aono (Riken, Japan)
2. Phaedon Avouris (IBM, USA)
3. Yoshio Bando - National Institute for Materials Science (NIMS) (Japan)
4. Flemming Besenbacher (Aarhus University, Denmark)
5. Mark Blamire (University of Cambridge, UK)
6. Guillermo Bozzolo (NASA Glenn Research Center, USA)
7. George Bourianoff (Intel, USA)
8. Roberto Car (Princeton University, USA)
9. Ignacio Cirac (Max-Planck Institut fur Quantenoptik, Germany)
10. Dongmin Chen (Rowland Institute for Science, Cambridge, MA, USA)
11. Wonbong Choi (Samsung, Korea)
12. Harold Craighead (Cornell University, USA)
13. Supriyo Datta (Purdue University, USA)
14. Cees Dekker (Delft University, Netherlands)
15. Pedro Echenique (Donostia International Physics Center (DIPC), Spain)
16. Andreas Engel (Basel University, Switzerland)
17. Leo Esaki (Shibaura Institute of Technology, Japan) - Invited Lecture
18. Fernando Flores (Universidad Autonoma de Madrid, Spain)
19. Harald Fuchs (Munster University, Germany)
20. Christoph Gerber (IBM, Switzerland)
21. James Gimzewski (UCLA, USA)
22. Herb Goronkin (Physical Research Laboratory - Motorola Labs, USA)
23. Masahiko Hara (Tokyo Institute of Technology, Japan)
24. James Heath (UCLA, USA)
25. Kikuji Hirose (Osaka University, Japan)
26. Christian Joachim (CEMES/CNRS, France)
27. Sajeev John (University of Toronto, Canada)
28. Dieter Kern (Tuebingen University, Germany)
29. Uzi Landman (Georgia Institute of Technology, USA)
30. Stuart Lindsay (Arizona State University, USA)
31. Daniel Loss (Basel University, Switzerland)
32. Ramesh G. Mani (Harvard University, USA)
33. Meyya Meyyappan (NASA, USA)
34. Seizo Morita (Osaka University, Japan)
35. Rodolfo Miranda (Universidad Aut�noma de Madrid, Spain)
36. Gernot Pomrenke (Air Force Office of Scientific Research, USA)
37. Calvin Quate (Stanford University, USA)
38. Jan van Ruitenbeek (Leiden University, Netherlands)
39. Lars Samuelson (Lund University, Sweden)
40. Christian Schoenenberger (Basel University, Switzerland)
41. Ivan Schuller (University of California, USA)
42. Clivia Sotomayor Torres (Wuppertal University, Germany)
43. Shen Tsai (NEC Fundamental Research Laboratories, Japan)
44. Christian Urbina (CEA-Saclay, France)
45. Luis Vina (Universidad Autonoma de Madrid, Spain)
46. Mark Welland (University of Cambridge, UK)
47. Stanley Williams (HP, USA)

From daemon Fri May 10 14:33:32 2002

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Greetings all.

I have come across a Gatan model 628/2 single tilt hot stage (1300C) and
heating control unit that was purchased for a Hitachi H600 100Kv TEM but
was never used. It was apparently tucked away in the lab by my predecessor
immediately after its purchase and was recently discovered after having
already replaced this microscope. I would like to know if anyone with a
similar microscope is interested in actually using it.

Please contact me off-line to discuss in more detail.

George R. Munzing Jr.
Engelhard Corporation
25 Middlesex-Essex Tpk.
Iselin, NJ 08830
TELE 732-205-7030
FAX 732-205-5300

From daemon Fri May 10 15:34:23 2002

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I would appreciate hearing from people taking color digital images. Is
there another slide presentation software, other than Powerpoint, that one
can use to show histology or fluorescence micrographs in a lecture or
meeting. The problem is pasting high resolution images to a Powerpoint
slide - quite often we have to reduce the size or the resolution of the
images and the images appear washed out. It defeats the purpose of using
high resolution cameras to capture images. Any
comments/suggestions/recommendations would be greatly appreciated.

Cora Bucana

Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747

From daemon Fri May 10 17:10:19 2002

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Try this:
http://www.google.com/search?hl=en&ie=UTF8&oe=UTF8&q=edwards+vacuum+evaporator&btnG=Google+Search

At 06:24 AM 5/10/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Fri May 10 18:46:04 2002

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Dear Cora

If you need some program just to show already prepared images (slide show)
you may try InfanView - this freeware software were discussed recently on
ListServer and received very high marks. Again, it's only for
demonstration, you may not create PowerPoint-like presentation in it. You
may show already prepared (even in Powerpoint I believe, not sure)
images. It's very good 'viewer'. It has some very nice features like - it
may shrink your image to the screen size and it's so quick. I am using it
to show my EM pictures 20-50 Mb in size...
http://www.irfanview.com/
Sergey

At 12:22 PM 5/10/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Fri May 10 18:50:48 2002

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I would wager that the problem isn't with PowerPoint, but rather the projector. Consider that most of the projectors in use now are 1024 x 728 pixels. The whole PowerPoint slide takes that up. If your slide in PowerPoint is set up for 10" x 7.5" (the default) and your image is put on the slide as a 4"x5", then whatever resolution your image was taken at is still only projected at 512x364 pixels.

Now consider using overheads -still not the best, but let's say that you have a good printer that you can print at 300 dpi. (Sublimation dye printers can do this and good quality inkjets can come close to doing this with 256 levels per color.) A 4"x5" print on the slide can still have a size of 1200 x 1500 pixels without loosing anything.

Add to this, the problems of projecting the full color range with projectors. I have read about this, but am not qualified to discuss it.

My advice is to stay with overhead transparencies and forget the fancy slide effects that you can do with PowerPoint or other presentation software.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

-----Original Message-----
} From: Corazon D. Bucana [mailto:bucana-at-audumla.mdacc.tmc.edu]
Sent: Friday, May 10, 2002 3:22 PM
To: Microscopy-at-sparc5.microscopy.com

I would appreciate hearing from people taking color digital images. Is
there another slide presentation software, other than Powerpoint, that one
can use to show histology or fluorescence micrographs in a lecture or
meeting. The problem is pasting high resolution images to a Powerpoint
slide - quite often we have to reduce the size or the resolution of the
images and the images appear washed out. It defeats the purpose of using
high resolution cameras to capture images. Any
comments/suggestions/recommendations would be greatly appreciated.

Cora Bucana

Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747

From daemon Fri May 10 18:55:30 2002

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Hi Corazon....

You might try using the slideshow function available in Irfanview
freeware.

www.irfanview.com

Best,

Angela

-----------------------------------------
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
-----------------------------------------

On Fri, 10 May 2002, Corazon D. Bucana wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would appreciate hearing from people taking color digital images. Is
} there another slide presentation software, other than Powerpoint, that one
} can use to show histology or fluorescence micrographs in a lecture or
} meeting. The problem is pasting high resolution images to a Powerpoint
} slide - quite often we have to reduce the size or the resolution of the
} images and the images appear washed out. It defeats the purpose of using
} high resolution cameras to capture images. Any
} comments/suggestions/recommendations would be greatly appreciated.
}
} Cora Bucana
}
} Corazon D. Bucana, Ph.D.
} Dept. Cancer Biology
} UT M.D.Anderson Cancer Center
} 1515 Holcombe Blvd, Box 173
} Houston, Texas 77030
} Phone: 713-792-8106
} FAX 713-792-8747
}
}

From daemon Fri May 10 19:44:53 2002

Contents Retrieved from Microscopy Listserver Archives
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Hi Cora,
I've used a piece of software called "UPresent" - its very good for
multi-media work, but the last version I used was a little light on text and
outlining capabillities. Here is the URL:

http://www.codeblazer.com/products.html

-Brad

----------
From: Corazon D. Bucana
Sent: Friday, May 10, 2002 12:22 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: slide presentation software

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
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I would appreciate hearing from people taking color digital images.
Is
there another slide presentation software, other than Powerpoint,
that one
can use to show histology or fluorescence micrographs in a lecture
or
meeting. The problem is pasting high resolution images to a
Powerpoint
slide - quite often we have to reduce the size or the resolution of
the
images and the images appear washed out. It defeats the purpose of
using
high resolution cameras to capture images. Any
comments/suggestions/recommendations would be greatly appreciated.

Cora Bucana

Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747

From daemon Sat May 11 07:42:01 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everybody - using a JEOL 5900LV, recently replaced tungsten filament,
got great images, few days later image looked like I was in backscatter
mode instead of secondary. Stubs were coated 360ang of Au, stubs are
flush with sample holder; 20kv, WD 8mm, ss 37 - looking at diatoms
1)aligned gun
2)checked the wobbler
Any suggestions of what is going on?
Thanks
Barb

From daemon Sat May 11 10:44:55 2002

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} From you post, I get the impression that you are reducing
the resolution of the image before inserting into
Powerpoint. This is one way to make image fit into the slide
image area, but does decrease the resolution of the
projected image. You can insert the image at full
resolution, then size the image on the slide using the
Format Image function.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870

From daemon Sat May 11 19:49:21 2002

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I second Scott Walck's comments and add a thought.

The limitation is most likely the projector technology. You are limited to
1024x768 resolution input on the newer projectors. There might still be a
question as to whether all that input ends up as output. I have an adapter
to take computer input (maybe even up to 1280x1024) and convert it to make
it suitable for a video projector. But that projector can only handle NTSC
which is a little worse that VGA (640x480) resolution. So all I put in
definitely does not make it out there. I also know the color rendition is
rather poor.

Scott suggested overheads as an option. I agree and might suggest 35mm
slides as another alternative. Our campus has (or at least had) a
centralized slide maker that could take Power Point or any printed output
and render it at 4000x3000 pixels, IIRC.

But will your audience be able to resolve that much detail from where they
are sitting?

I would like to hear from someone who can speak authoritatively on the
physiology of the matter. My own sense is that anything more than 1024
pixels across will not be appreciated from normal viewing distance.
1024-pixel images may seem pixelated to someone standing a few feet away
from the screen, but I doubt that a viewer sitting in the front row would
notice.

For that reason, I take the approach of simply recording multiple images
from our SEMs at 1024-pixels across showing the appropriate level of detail
in each image. A 40-MB image certainly contains lots of information;
however, it can really only be appreciated one 1024-pixel image at a time.
I suggest that is the way the presentations will need to be prepared.

At 03:22 PM 5/10/02 -0400, you wrote:

} I would appreciate hearing from people taking color digital images. Is
} there another slide presentation software, other than Powerpoint, that one
} can use to show histology or fluorescence micrographs in a lecture or
} meeting. The problem is pasting high resolution images to a Powerpoint
} slide - quite often we have to reduce the size or the resolution of the
} images and the images appear washed out. It defeats the purpose of using
} high resolution cameras to capture images. Any
} comments/suggestions/recommendations would be greatly appreciated.
}
} Cora Bucana
}
} Corazon D. Bucana, Ph.D.
} Dept. Cancer Biology
} UT M.D.Anderson Cancer Center
} 1515 Holcombe Blvd, Box 173
} Houston, Texas 77030
} Phone: 713-792-8106
} FAX 713-792-8747

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking

From daemon Mon May 13 01:01:43 2002

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I am interested in opinions on how long it takes to prepare a routine
chemically fixed, resin embedded biological sample for TEM (by an average
technician). I need to work out how many samples could reasonably be
prepared by one person in a year.

Thanks
Cathy Gillespie

From daemon Mon May 13 01:01:48 2002

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Hi Chen,

I don't know the 420 but as there have been no other
responses maybe I can shed some light on your question.

} From memory, when we had our Philips 300 the filament was
always on at a low level, the filament control increased
this level but never switched it off. I guess it was
interlocked with the vacuum or something, I'm a little hazy
about the details. On the back of the console was a switch
(I think marked high and low) that would adjust the current
setting.

It sounds like your 420 is similar, ie. there is a heating
current even though the filament control is off. As soon
as you have HT you have emission. It may be that this batch
of filaments have slightly thinner wire or that the
filament is not set correctly in the Wenhelt. If the
filament is set correctly and there is a switch for the
filament current that is set on 'high' try setting it to
'low'. If you can still saturate the filament on that
setting then that's OK.

Maybe the quiescient current circuit is playing up and
giving too high a current?

If that is not the case then I have no idea how you can get
a beam without heating unless you have field emission from
a very sharp tip on the filament. Not that likely at 120kV
but seen at 1MeV on HVEMs.

Good luck,
Ron

On Fri, 10 May 2002 14:39:57 GB
"Y.Chen-at-surrey.ac.uk"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} We have a Philips EM400T in our lab. It is old but still works well. However, I
} noticed that illumination can be observed as soon as High Tension is on. As a
} result, the saturation point is very low, only two or three steps of filament
} current. My question is why there is a clear emmission current without any
} filament current? Is this caused by a gun fault? What can we do about it?
} Thank you very much in advance!
}
} Yanling
}
} Dr.Y.L.Chen
} MicroStructural Studies Unit
} School of Engineering
} The University of Surrey
} Guildford, Surrey
} GU2 7XH, England
}
}
} ---------------------------------------------
} This message was sent using UNIS MailSystem.
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk

From daemon Mon May 13 04:40:36 2002

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Kathy,
It'll depend, how much you'll pay for it. If you offer $50-60K, S/he will
make a LOT of samples to you!!! There is no 'average' technician in
EM. EM is very sophisticated area and in order to have necessary
knowledge, experience etc, you have to spend 2-3-5 years. I am not talking
about 'hands' - it comes from God. It's not easy to find such qualified
person. 'Average' person will kill your most valuable sample in two
minutes... Good luck. Sergey

At 03:46 PM 5/13/02 +1000, you wrote:
} ------------------------------------------------------------------------
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From daemon Mon May 13 07:24:49 2002

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Dear Listers,
I am looking for programmes (preferably free!)that
will enable me to read jpeg files recovered after
deletion, or corruption of the file marker. I have
tried a few of the better known jpeg file readers
without success. Thanks, Jeremy
Reply to jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com

From daemon Mon May 13 10:19:30 2002

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Hi All,

If you want a scanner for smaller negs(35mm or slides), we have a Nikon
Super Coolscan 4000 scanner that does quite well and costs less. I
think it cost between 1&2K. Less expensive scanners I've used tend
to put lines in the image or have missing pixels.

Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas

Gary Radice wrote:
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} } } I am interested in the current negative-scanner
} } } technology, but I would like to find a non-contact
} } } scanner.
}
} If you have a robust budget, (12-18K$) you might check the Imacon
} scanners, either the Flextite 848 or Precision III.
}
} I have no commercial interest in these products.
} --
} Gary P. Radice gradice-at-richmond.edu
} Associate Professor of Biology 804 289 8107 (voice)
} University of Richmond 804 289 8233 (FAX)
} Richmond VA 23173 http://www.science.richmond.edu/~radice

From daemon Mon May 13 10:29:57 2002

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Microscopists,
Does anyone know of a way (quant. or qualitative) to meause the
amount of thickness coating a protein sample with platinum in a metal
evaporation
run? I don't have a quartz thickness monitor, so is there some
extrapolation you can do based on starting and ending material? Right
now all we do is
use filter paper wedges to estimate shadow thickness (not empirically).
Thank you.

Mike D.

From daemon Mon May 13 11:29:27 2002

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Hi,

Funny you should ask. I wrote a post last night but wasn't going to post it until tomorrow.

In the late 1960s there were charts called nomographs to graphically estimate the thickness of films evaporated onto substrates. They were kind of a graphical slide rule to figure out how much metal to evaporate onto a substrate.
I can post a better email from home but this is the link to a nomograph on the web.

http://www.technology.niagarac.on.ca/courses/phtn1432/NotesOnPreloadingSources.html

The scale at the top is the bulk density of the element or alloy. It is not labeled with the number scale, only the elements. Neither is carbon (near Be), Ge and some alloys like Au-Pd shown.

The formula used for these nomograph charts and converted to use bulk densities is:

wt = [4 * Pi * d� * t * rho * (10^-5)]/(sin s)

wt to use is in mgs
d is the distance from the point source to the sample target in cms.
t is the thickness in angstroms, �.
rho is the bulk density, 21.45 grams per cm� for Pt.
10 EE -5 is to get the units right.
s is the shadowing angle measured at the target (as I recall) and that is subtended by the point source and the plane of the sample.

It is easy to derive this formula from surface area concepts and densities.

For straight down evaporations, sin 90� = 1. The above nomograph doesn't have a scale for shadowing angle. The formula will tell you how to correctly ESTIMATE the amount of material you used.

Hope this helps.

Paul Beauregard
Senior Research Associate
PPG Industries
Monroeville Technical Center
Monroeville, PA 15146

-----Original Message-----
} From: Mike Delannoy [mailto:delannoy-at-jhmi.edu]
Sent: Monday, May 13, 2002 11:20 AM
To: microscopylistserver

Microscopists,
Does anyone know of a way (quant. or qualitative) to meause the
amount of thickness coating a protein sample with platinum in a metal
evaporation run?
I don't have a quartz thickness monitor, so is there some
extrapolation you can do based on starting and ending material? Right
now all we do is use filter paper wedges to estimate shadow thickness (not empirically).
Thank you.

Mike D.

From daemon Mon May 13 14:50:50 2002

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Hello Jeremy

Is it possible to recover corrupted JPEG file specifically? Never hear
about that. I could talk about PC/Win platform only. Windows NT itself
(NTFS file system) has internal ability to recover corrupted files
(any). It's called 'checkdisk' I believe. It's in the standard Windows
package. Similar things should be in win2000. You may also run Norton Disk
Doctor on any Win platform - it works great. The things about that: all
these programs are not specific to the JPEG files. So, I am not sure how
it may help in your particular case. By the way, for PC/Win I would
strongly recommend to have Norton Utilities on HD: very useful stuff. Sergey

At 01:16 PM 5/13/02 +0100, you wrote:
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From daemon Mon May 13 15:59:45 2002

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Greetings Folks,

I'd like to echo Ron's advice regarding checking that the filament is set
up correctly in the wehnelt and that it meets the manufacturers specs
(thickness, material, etc). The type of problem you described can be caused
by any number of faults in the HT circuitry of your 400T, but your first
and most likely source, and easiest and least expensive to fix, would be a
filament exchange. If that doesn't cure your problem I would recommend
having a qualified service engineer in to analyse the situation. We once
had a similar problem on our CM12 (which has a nearly identical HT set) and
after cleaning the emission chamber, cleaning then replacing the gun, and
just before exchanging the HT generator tank, we were able to prove that
our cable was breaking down at voltages greater than 40KV. This created all
kinds of bias problems which manifested with the symptoms you have
described. When the problem first started occurring, it was suspected that
we had a malfunction in the emission section of our HT tank. The filaments
could be set very far back in the wehnelt assembly to achieve somewhat
"normal" heating and illumination behaviour, but changing the Emission
setting then had negligible impact on beam current. If these symptoms are
what you are noticing with your TEM, you might want to check the HT cable.
This is probably best accomplished with a meter that will measure
inductance, checking for high impedance "short circuits" amongst the three
conductors of the cable. Considerable disassembly may be required to do
this properly, (to isolate both ends of the cable) hence the
recommendation to call in the service representative.

Good Luck!!!

Lee

From daemon Mon May 13 17:49:04 2002

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On our Philips 400T we had a switch on the back of the left console
that was marked Low/High, the low settng was used for tungsten
filaments and the high setting for LaB6 filaments which required more
current to heat the crystal. It may be that this switch was
accidently bumped to the high position?

At 4:49 PM -0400 5/13/02, Leland G Hanna wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html

From daemon Mon May 13 18:29:39 2002

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Sergey points out some good facets of file recovery.

What is missing from your post is whether you are using
FAT16, FAT32 or NTFS. In a "normal" mode, the OS
does not delete the file, but rather, it "marks" it as deleted.
This is done by replacing the file name with an initial ? mark.
There are variations on this theme. But unless and until
the disk is defragmented, the original file is still on the drive.
(a good reason to use Norton WipeDisk if you don't want
it to remain there) But there are exceptions, of course.

I agree that Norton DiskDoctor is the way to go. This will
allow recovery (un-deletion) of files. But beware that if you
delete a file and do other disk actions, recovery may be
impossible. This is because the prior-used sectors are now
marked as available for use. So if you delete a file, then
add new files, all or portions of your deleted file's sectors
could be used--eliminating any possibility of recovery.

gary g.

At 12:58 PM 5/13/2002, you wrote:

} Hello Jeremy
}
} Is it possible to recover corrupted JPEG file specifically? Never hear
} about that. I could talk about PC/Win platform only. Windows NT itself
} (NTFS file system) has internal ability to recover corrupted files
} (any). It's called 'checkdisk' I believe. It's in the standard Windows
} package. Similar things should be in win2000. You may also run Norton
} Disk Doctor on any Win platform - it works great. The things about that:
} all these programs are not specific to the JPEG files. So, I am not sure
} how it may help in your particular case. By the way, for PC/Win I would
} strongly recommend to have Norton Utilities on HD: very useful stuff. Sergey
}
} At 01:16 PM 5/13/02 +0100, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From daemon Mon May 13 18:46:25 2002

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Thank you for all of your input!

I finally found the software I was looking for. This information was
forwarded to me by Michael Nesson, Ph.D. at OSU and a similar message was
sent by John Mardinly.

"The company is called Alicona Imaging GmbH. The software product is called
MeX. Try www.alicona.com for additional information."

A demo version is available online and the software looks very impressive
but the cost is several $K. Thank you for the help.

Dan

****************************************************************************
******************************************************************
Daniel D. Smolko, Ph.D.
Senior Research Scientist & BioChemical Engineer
Nanogen, Incorporated
10398 Pacific Center Court
San Diego, CA 92121

Tel.# (858) 410-4798
Fax.# (858) 410-4848
e-mail: dsmolko-at-nanogen.com

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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From daemon Mon May 13 23:24:30 2002

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Thank you to all who responded to my request for information regarding
other software for slide presentation. Some of the suggestions include:
(a) http://www.irfanview.com I downloaded this tonight and I find it very
easy to use.
(b) UPresent from http://www.codeblazer.com/products.html I have not
tried this yet
(c) Sliderite
(d) using HTML
(e) JPEGView
(f) Other suggestions deal with increasing the computer RAM and data
compression.

Our projector has a 1024 x768 resolution and we do paste the full image
onto the Powerpoint page and then adjust the size of the image but the
problem comes when we try to paste a composite consisting of more than 6
images. Quite often the images appear washed out - the images were
captured originally in Tiff format but as I indicated earlier we had to
compress the file before pasting.

Thank you all for the information. I will try some of the other
suggestions soon.

Cora Bucana

Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747

From daemon Tue May 14 07:11:15 2002

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Here here, i recently left clinical area for reseach. the head cyto tech
convinced the administrator that he could do the job as good as i could. in
six months he trashed the lab 3 diamond knives, and an LKB. an average EM
tech. is probly a bad em tech.
as for your question. i have seen labs that do 1000/year with just 1.5 techs.
i wouldn't want to work in one of those mills.
john

From daemon Tue May 14 07:58:40 2002

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Hi

Long time lurker, first time poster (well, almost).

We recently upgraded from a video camera to a cooled digital camera that is
very sensitive. While doing epifluorescence on our Nikon TE300, we noticed
some rather significant variations in the illumination from the mercury
lamp, specifically a hot spot filling about 1/4 of the screen. This is not
visible through the eyepiece and is not visible with the video camera. I
have tried re-aligning the lamp in various ways. This moves the hot spot
around but cannot make it go away. I even tried putting a diffuser in the
light path. The hot spot is still there. I've had the vendors in, who say
we might need a lamp with a bigger arc to fill the field of view.

Does anybody have any suggestions?

Eric

From daemon Tue May 14 09:54:12 2002

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Greetings,

The following note is posted for a friend who does not have access to the
list server;

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313

A colleague is looking for someone to install an Amray
3600 FESEM. Please reply directly to

James Greer
PVD Products
231 Andover St.
Wilmington, MA 01887
(978) 694-9455
jgreer-at-pvdproducts.com

Thanks,
Audrey Dow

From daemon Tue May 14 10:46:09 2002

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Eric,

You might be imaging a reflection off of the front of some part of the
camera/lens system. Check to make sure that there is no contamination on the
forward side of any of the accessible optical elements that could serve as a
source of scattered light that would be reflected by another optical element.

John Twilley

Eric Johnston wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi
}
} Long time lurker, first time poster (well, almost).
}
} We recently upgraded from a video camera to a cooled digital camera that is
} very sensitive. While doing epifluorescence on our Nikon TE300, we noticed
} some rather significant variations in the illumination from the mercury
} lamp, specifically a hot spot filling about 1/4 of the screen. This is not
} visible through the eyepiece and is not visible with the video camera. I
} have tried re-aligning the lamp in various ways. This moves the hot spot
} around but cannot make it go away. I even tried putting a diffuser in the
} light path. The hot spot is still there. I've had the vendors in, who say
} we might need a lamp with a bigger arc to fill the field of view.
}
} Does anybody have any suggestions?
}
} Eric

From daemon Tue May 14 13:30:23 2002

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (giummc-at-rpi.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, May
14, 2002 at 12:37:15
---------------------------------------------------------------------------

Email: giummc-at-rpi.edu
Name: Cindie Giummarra

Organization: Rensselaer Polytechnic Institute

Education: Graduate College

Location: Troy, New York, USA

Question: Hi,

I was wondering which techniques I could use to detect calcium in
15-40ppm amounts in an aluminum alloy?

I am interested in finding out what form the Ca is in and where it is
located in the microstructure.

Thank you.

---------------------------------------------------------------------------

From daemon Tue May 14 14:04:33 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

O.k. this is not mciroscopy directly BUT I hope someone out there has an
answer.

We're looking for an "adhesive" to glue #3 Whatman filter paper to
stainless steel (surface area of ~ 3 sq cm or less) but it must be: (1) sterile or
sterilizable, (2) water stable, (3) biologically non-toxic.

Thanks for your suggestions!

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

From daemon Tue May 14 14:05:06 2002

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HI Listers,

We have an old Nikon Labophot2 and we are in need of a trinoc head. It is currently a dual head scope but we need to convert it so it can handle a digital camera. Currently we have a TriPix RGB (hanging off one of the eyepieces with an adapter) but we're having trouble with dust and spots at 100X oil. I know this is not the optimum configuartion.

Does any one have a suggestion about digital cameras for light microscopes? We are working mostly with H&E (10 -40X) and some blood smears (100X oil). We will be saving images to a website.

Also, I'm looking for anybody who might have a old trinoc head for a Nikon Labophot2 sitting around that might want to sell it.

I'm welcome to all ideas, so send them along.

In the new land of tornadoes,

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

From daemon Tue May 14 17:18:25 2002

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Silicone or epoxy compound? Both of them are 'naturally' aseptic-
aggressive enough to kill any bacteria unless polymerized.... Silicone
when completely polymerized (be sure) is bio-compatible. I mean silicone
compound with clear silicone base and strong smell of acetic acid. Sergey

At 11:58 AM 5/14/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Tue May 14 17:49:12 2002

Contents Retrieved from Microscopy Listserver Archives
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I haven't had a chance to test this but I saw a very impressive
demonstration by Adobe using their Acrobat program (not the free Acrobat
Reader). Creating PDF files and editing them with this software is quite
easy and allows almost any program for creation.

From daemon Tue May 14 22:36:36 2002

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Also, properly cured Silicone compounds mostly compatible with hot water,
but not with hot steam on repeated exposure, especially pressure seals.
Sterilize by boiling, not autoclaving. Try www.nusil.com for more
information.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, May 14, 2002 6:10 PM

Surprising: silicone rubber (after polymerization of the silicone compound)
is extremely stable against the temperature. It widely used in high
temperature applications (simplest example: silicone compound for car
engines). 121oC which is typical autoclave temperature should not hurt
silicone at any instances even with overheated steam... I also check WEB
site you suggested and did not find anything about thermal instability of
the silicones... Moreover, the curing temperature they suggested is 150oC
for 5 hours, which is much longer than autoclave procedure (20-30 min
usually). If I remember correctly, people used silicone compound to make
molds for the metal... I am sorry, could not agree with you: as I know,
silicone is most thermally stable rubber suitable for biological/medical
use. Actually, my point was that if you perform procedure at the sterile
conditions (laminar cabinet with HEPA filter for instance) you don't have
to sterilize the compound. Compound is sterile itself. You DO have to
care about sterility of the other parts, but non-polymerized silicone. Sergey

At 08:26 PM 5/14/02, you wrote:
} Also, properly cured Silicone compounds mostly compatible with hot water,
} but not with hot steam on repeated exposure, especially pressure seals.
} Sterilize by boiling, not autoclaving. Try www.nusil.com for more
} information.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
} (678)467-0012 mobile
}
} This message is made of 100% recycled electrons.
} ----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, May 14, 2002 6:10 PM
} Subject: Re: Biosafe adhesive
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Silicone or epoxy compound? Both of them are 'naturally' aseptic-
} } aggressive enough to kill any bacteria unless polymerized.... Silicone
} } when completely polymerized (be sure) is bio-compatible. I mean silicone
} } compound with clear silicone base and strong smell of acetic acid. Sergey
} }
} } At 11:58 AM 5/14/02, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } O.k. this is not mciroscopy directly BUT I hope someone out
} there
} } } has an
} } } answer.
} } }
} } } We're looking for an "adhesive" to glue #3 Whatman filter paper
} to
} } } stainless steel (surface area of ~ 3 sq cm or less) but it must be: (1)
} } } sterile or
} } } sterilizable, (2) water stable, (3) biologically non-toxic.
} } }
} } } Thanks for your suggestions!
} } }
} } }
} } }
} } } Richard E. Edelmann, Ph.D.
} } } Electron Microscopy Facility Supervisor
} } } 352 Pearson Hall
} } } Miami University, Oxford, OH 45056
} } } Ph: 513.529.5712 Fax: 513.529.4243
} } } E-mail: edelmare-at-muohio.edu
} } } http://www.emf.muohio.edu
} } }
} } } "RAM disk is NOT an installation procedure."
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
} }

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Wed May 15 04:29:13 2002

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
We have a GIF here attached to a JEOL 3010 and something strange has
happened to the energy selecting slit. It is far too wide (like a measured
width of about 70 eV, when nominally set to 1eV). Is there any user
intervention that can fix this problem easily? (e.g. Does anyone understand
how to use the Slit Width Calibration function in Filter Control, and would
this help?)

Or, is this simply a case for calling the Gatan Service Engineer?

Thanks in advance for any help you can give.

Best wishes

Ian MacLaren
N.B. New address
FB Materialwissenschaft - FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162893
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/

From daemon Wed May 15 05:48:00 2002

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Dear all,
Has anybody out there installed a TV Camera for HREM imaging ABOVE the
normal viewing screen in a TEM?

We would like to install a TV Camera on a JEOL TEM for focussing HREM
images, but there's no space below the Camera due to other attachments being
there. Meanwhile, there are a couple of ports free above the viewing
screen.

If anyone has any ideas of good cameras / camera manufacturers for this
purpose, please contact me.

Thanks

Ian MacLaren
N.B. New address
FB Materialwissenschaft - FG Strukturforschung
Petersenstr. 23, 64287 Darmstadt, Germany
Tel: +49 6151 162893
ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/

From daemon Wed May 15 07:26:57 2002

Contents Retrieved from Microscopy Listserver Archives
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Previous and present ETEC owners,
Does anyone have an ETEC micromanipulator hidden away in a drawer or
cabinet? One of my customers has my only complete one and needs a
second for a project. Beg, borrow, rent or buy are all options. Thanks.

Ken Converse
owner
Quality Images
third party SEM service
16 Creek Rd.
Delta, PA 17314

717-456-5491

From daemon Wed May 15 07:46:10 2002

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Morning Eric,
Just a guess, but one of the things that is emitted in large
quantity is heat (ir?). In the old days when bright light in microscopy
always came from a carbon arc, the UV was filtered thru a 1"+ layer of
copper sulfate solution to cool off the light (remove the ir?).
Now, if I remember, most CCD detectors have a sweet spot in the near
or far red (ir?). My guess is that you may be 'seeing' with your CCD what
YOU cannot see during visual alignment.
I believe that there are barrier filters or N.D. filters in front of
the Hg lamp that will accomplish what you want.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

} ----------
} From: Eric Johnston
} Sent: Tuesday, May 14, 2002 8:50 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Hot spot in epifluorescence
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi
}
} Long time lurker, first time poster (well, almost).
}
} We recently upgraded from a video camera to a cooled digital camera that
} is
} very sensitive. While doing epifluorescence on our Nikon TE300, we
} noticed
} some rather significant variations in the illumination from the mercury
} lamp, specifically a hot spot filling about 1/4 of the screen. This is
} not
} visible through the eyepiece and is not visible with the video camera. I
} have tried re-aligning the lamp in various ways. This moves the hot spot
} around but cannot make it go away. I even tried putting a diffuser in the
} light path. The hot spot is still there. I've had the vendors in, who
} say
} we might need a lamp with a bigger arc to fill the field of view.
}
} Does anybody have any suggestions?
}
} Eric
}
}
}

From daemon Wed May 15 08:05:43 2002

Contents Retrieved from Microscopy Listserver Archives
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You may want to try SIMS [Scanning Ion Mass Spectroscopy] [EDX may only be
able to do about 1,000 ppm, but that is sample dependent and matrix
dependent.]

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: giummc-at-rpi.edu [mailto:giummc-at-rpi.edu]
Sent: Tuesday, May 14, 2002 2:20 PM
To: Microscopy-at-sparc5.microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (giummc-at-rpi.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, May
14, 2002 at 12:37:15
---------------------------------------------------------------------------

Email: giummc-at-rpi.edu
Name: Cindie Giummarra

Organization: Rensselaer Polytechnic Institute

Education: Graduate College

Location: Troy, New York, USA

Question: Hi,

I was wondering which techniques I could use to detect calcium in
15-40ppm amounts in an aluminum alloy?

I am interested in finding out what form the Ca is in and where it is
located in the microstructure.

Thank you.

---------------------------------------------------------------------------

From daemon Wed May 15 08:46:32 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

We have an EDS detector attached to a 200 kV TEM that is having its crystal
changed (to a Si(Li)).
The original resolution was 139 eV. Now, depending on the amount of the
repair, it could become 138 or 143 eV.
My question is : is 143 eV worse than 138 eV in practise ? Does one notice
the difference ?

I appreciate any ideas.

Isabel

Isabel Nogueira
Lab Technicien
Instituto Superior T�cnico
Materials Department
Avenida Rovisco Pais
1049-001 Lisboa
Portugal
tel.: +351 218418123
fax: +351 218418120
email: isabeln-at-popsrv.ist.utl.pt

From daemon Wed May 15 09:19:01 2002

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I suppose the issue depends on how you use your detector and which elements
you examine. If it is for elements up through arsenic, you should have
little trouble with overlaps and 143 eV resolution ought to be fine. If you
are looking at heavier metals with lots of overlaps, you may want to hold
out for the better resolution. For example, last week I was looking at a
Pb-Bi-Sn-Cd fusible alloy. That made for plenty of overlaps. Even so, I
think my results were good even without operating in the highest resolution
mode.

Our EDS offers 20, 10, and 5 eV/channel modes. Thus you are only talking
about a difference of 1/4th of a channel in the 20 eV/channel mode which we
use most of the time. I don't think you would notice it. But that is only
my guess.

Warren

At 02:39 PM 5/15/02 +0100, you wrote:

} Dear colleagues,
}
} We have an EDS detector attached to a 200 kV TEM that is having its crystal
} changed (to a Si(Li)).
} The original resolution was 139 eV. Now, depending on the amount of the
} repair, it could become 138 or 143 eV.
} My question is : is 143 eV worse than 138 eV in practise ? Does one notice
} the difference ?
}
} I appreciate any ideas.
}
} Isabel
}
} Isabel Nogueira
} Lab Technicien
} Instituto Superior T�cnico
} Materials Department
} Avenida Rovisco Pais
} 1049-001 Lisboa
} Portugal

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking

From daemon Wed May 15 09:35:06 2002

Contents Retrieved from Microscopy Listserver Archives
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} O.k. this is not mciroscopy directly BUT I hope someone out there
} has an
} answer. We're looking for an "adhesive" to glue #3 Whatman filter paper to
} stainless steel (surface area of ~ 3 sq cm or less) but it must be: (1)
} sterile or sterilizable, (2) water stable, (3) biologically non-toxic.
}
} Richard -

My son-in-law makes and repairs diamond scalpels (he started with diamond
EM knives, but decided that surgeons are less fussy & more clumsy than
microscopists). He, and the other manufacturers, cements the blades into
steel or titanium handles with a 2-part epoxy glue that can take autoclave
temperatures.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

From daemon Wed May 15 09:42:14 2002

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I have recently been thinking of purchasing the Nikpn Coolpix 995 for
digital photgraphy but I read some of the thread from last year
regarding attaching this camera to a microscope. I have attached it and
for what I was doing at the time it was suitable. Can anyone suggest
other digital cameras in the same price range that will do microscopy
work (compound and dissecting)(macro and micro), good outside and indoor
photos and can be used to photograph electrophoresis gels. Thanks
Debbie

From daemon Wed May 15 09:50:36 2002

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Cathy

As stated previously, your average tech would not be a good thing. I have been
in a clinical lab for the past 18 years. When I first came to this lab it had 3
techs, myself included with no less then 8 years EM experience each. We were
processing around 1850 specimens per year with a turn around time of less than
48 hours (the specimens had been in glut for at least overnight). About half of
these were cut and viewed for diagnosis. This is an average of 7 processed per
day and 4 per day being cut and viewed. Some days were more some less. I
remember at least one day where we processed 15 specimens. So as you can see,
the question you should ask is not how many specimens can be processed, but how
many can be cut and scoped successfully in a day. Anyone that can follow a
protocol can process but only an experienced tech can successfully cut, stain,
scope, and print a specimen. If s/he is the only tech in the lab, then IMHO the
most one could expect would be maybe 4 or 5 specimens per day if s/he were doing
everything (the processing, cutting etc) and this would be pushing the limit to
do everyday day in and day out. If the specimens were in blocks, then one may be
able to do 8 or more in a day depending on the number of pictures taken and the
speed of the print processor in the darkroom, again not something I would want
to do every day. If your images are acquired digitally, then that would need to
be accounted for in the scheme of things.

Mannie Steglich
Tech Dir E M Lab
U T M D Anderson Cancer Center

From daemon Wed May 15 10:00:31 2002

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I'm trying to help a colleague refine their technique for a 3D
reconstruction project. They work with kidney. They are trying to
reconstruct all of the thin loops of Henle (approx 15um in diameter)
through a depth of about 3-4 mm. In a nutshell, their current protocol is:

* formaldehyde fix & process kidney tissue for TEM (w/o OsO4)
* embed in Spurrs (normal hardness)
* cut 1um sections with a glass knife, sampling every 5um
* etch the epoxy from the sections and perform immunohistochemistry
* capture images of the sections and software align them for 3D reconstruction

Their biggest complaint is aligning the sections. My thought was that this
might be as a result of compression due to sectioning (the block faces are
pretty large). Occasional wrinkles in the plastic don't help matters.

As we talked about the problem there were a couple of issues that I
raised. I'd appreciate feedback from the list, since I've not done any 3D
work before.

* would a harder formulation of Spurrs be a better choice?
* would a different epoxy altogether be a better choice?
* if a different epoxy, how about something like LRGold (apologies to vendors
of competing products), a methacrylate-type plastic that could be embedded
at a lower temp and might be better overall for their immuno-reactions (but
might be more prone to shrinkage and/or compression artifacts)?

Yours,
Doug Cromey

....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: Cromey-at-Arizona.edu) :
:...................................................................:
http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"

From daemon Wed May 15 10:17:35 2002

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Hi all

wow, I got a lot of really useful replies.

The majority suggest that there is some kind of reflection on one or more
of the lens surfaces that's causing the hot spot, especially from the
camera adapter (in my case, the adapter has no lens so that's out).

The other suggestion was that the hot spot is due to IR, which CCDs can be
especially sensitive to. I need to find out if there is an IR blocker in
light path or on the camera itself.

Thanks for all the helpful suggestions. I'll keep you updated.

Eric

From daemon Wed May 15 10:55:40 2002

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} I have a relatively old Wild dissecting scope that needs
} repair/service. Does anyone know a good place to have that done in the
} Bay area, preferably near Stanford? Is this the kind of thing that
} Universities typically have standing contracts with someone for?

thanks,

Taylor

From daemon Wed May 15 12:40:34 2002

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Maybe more important than the quoted detector resolution is how you choose
to run the EDS system. Using small amplifier time constants and high
counting high rates can degrade the actual resolution a lot more than just a
few eV.

For historic reasons, EDS detector resolution is quoted at the energy of Mn
K-alpha x-rays (5.4 keV). However, the EDS resolution is actually has
energy dependent and energy independent contributions, so the x-ray peaks at
O-K, for example will be much narrower than those at Mn or Fe-K. Degrading
the detector resolution by adding (energy independent) electronic noise will
broaden the low-energy peaks much more than it does the high-energy ones.

Warren's comment about heavy elements and peak overlaps might need
clarification. For EDS of heavy elements in a TEM (as opposed to an SEM),
you can use the K or L-shell x rays with energies up to 20, 40 keV, or even
higher energies to avoid significant overlaps. The problem with peak
overlaps arises when you want to include light elements in the analyses and
the K-peaks from those elements overlap with higher-shell x-rays (e.g., L
or M) from the heavy elements in your sample. In that case, you might want
better resolution.

Larry

----------
From: Warren E Straszheim
Sent: Wednesday, May 15, 2002 7:11 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: Re: EDS detector resolution

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I suppose the issue depends on how you use your detector and which
elements
you examine. If it is for elements up through arsenic, you should
have
little trouble with overlaps and 143 eV resolution ought to be fine.
If you
are looking at heavier metals with lots of overlaps, you may want to
hold
out for the better resolution. For example, last week I was looking
at a
Pb-Bi-Sn-Cd fusible alloy. That made for plenty of overlaps. Even
so, I
think my results were good even without operating in the highest
resolution
mode.

Our EDS offers 20, 10, and 5 eV/channel modes. Thus you are only
talking
about a difference of 1/4th of a channel in the 20 eV/channel mode
which we
use most of the time. I don't think you would notice it. But that is
only
my guess.

Warren

At 02:39 PM 5/15/02 +0100, you wrote:

} Dear colleagues,
}
} We have an EDS detector attached to a 200 kV TEM that is having its
crystal
} changed (to a Si(Li)).
} The original resolution was 139 eV. Now, depending on the amount of
the
} repair, it could become 138 or 143 eV.
} My question is : is 143 eV worse than 138 eV in practise ? Does one
notice
} the difference ?
}
} I appreciate any ideas.
}
} Isabel
}
} Isabel Nogueira
} Lab Technicien
} Instituto Superior T�cnico
} Materials Department
} Avenida Rovisco Pais
} 1049-001 Lisboa
} Portugal

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of
materials
Computer applications and networking

From daemon Wed May 15 12:45:00 2002

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} O.k. this is not mciroscopy directly BUT I hope someone out there
} has an
} answer. We're looking for an "adhesive" to glue #3 Whatman filter paper to
} stainless steel (surface area of ~ 3 sq cm or less) but it must be: (1)
} sterile or sterilizable, (2) water stable, (3) biologically non-toxic.
}
} Richard -

My son-in-law makes and repairs diamond scalpels (he started with diamond
EM knives, but decided that surgeons are less fussy & more clumsy than
microscopists). He, and the other manufacturers, cements the blades into
steel or titanium handles with a 2-part epoxy glue that can take autoclave
temperatures.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

From daemon Wed May 15 14:59:01 2002

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Hello,
Our glow discharge unit is becoming unreliable, and we are thinking about
replacing it. We have contemplated having a dedicated stand alone unit versus
going with a large vacuum evaporator multi-function system. I send this
message out so that vendors can respond directly to me, and also as
a solicitation for what
technology has/hasn't worked for others.
Thanks in advance,
Randy Nessler
University of Iowa
Central Microscopy Research Facility
Iowa City, Iowa 52358
Phone 319-335-8142
Fax 319-384-4469

From daemon Wed May 15 17:38:25 2002

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Just another posting about digital microscope adaptors. I am looking for informatio leading to the ordering and purchase of an adaptor for the Olympus E20N digital camera.

At the college lab where I will be teaching as an adjunct this summer, a new toy has been purchased: an Olympus E20N digital camera. Some discussion has focused on purchase of a microscope attachment. The attachment that has been recommended by one of the staff attaches to the 62mm filter ring threads of the camera. I have two scopes that I use in teaching, each of them w/ c. 45. eyetube inclination angles; there is no trinocular tube available. I fear the forces of attaching a camera to a scope by the filter ring at such an angle would be unhealthy for the camera. The camera is a hefty one.

Alan Davis

--
Alan E. Davis
Science Department
Marianas High School
PMB 30, Box 10006,
Saipan, MP 96950
Northern Mariana Islands
adavis-at-saipan.com

"An inviscid theory of flow renders the screw useless, but the need
for one non-existent."
---Lord Raleigh(aka John William Strutt),or else
his son, Jr., who was also a scientist.

From daemon Wed May 15 17:51:34 2002

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Dear Debbie,
I recently saw a new Sony camera with a large lens that can focus down to
about one inch and claims to have better low-light performance than most
digitals. It is a 5 megapixel and sells for about what the Nikon 995 did
when it was first introduced (~$1650.00CAN). I'm sorry, I don't know the
model number, but it is hard to miss, since it looks funny with a tiny
digital camera body attached to a large, complex optical lens. It might best
suit your multiple needs.
At 10:35 AM 5/15/02 -0400, you wrote:

} I have recently been thinking of purchasing the Nikpn Coolpix 995 for
} digital photgraphy but I read some of the thread from last year
} regarding attaching this camera to a microscope. I have attached it and
} for what I was doing at the time it was suitable. Can anyone suggest
} other digital cameras in the same price range that will do microscopy
} work (compound and dissecting)(macro and micro), good outside and indoor
} photos and can be used to photograph electrophoresis gels. Thanks
} Debbie
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca

From daemon Wed May 15 18:08:05 2002

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Engineer Senior/Principal

Arizona State University Center for Solid State Science is seeking
qualified candidates to fill the position Engineer Senior or Principal.
Level and salary will be determined based on qualifications. This is a
full time, benefit eligible position. This position is responsible for
maintaining high levels of performance in analytical high-resolution
electron microscopes particularly electrical, magnetic and mechanical
stability, while concurrently developing these systems to achieve higher
levels of performance, including computer interfacing.

Complete qualifications and application information please refer to SR
108236 at www.asu.edu/hr/jobs/. Application deadline is May 28, 2002 and
every two weeks thereafter until search is closed.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704

Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu

From daemon Thu May 16 01:07:34 2002

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Hi

I am not at my office at the moment (using webmail) so I cannot check directly
but I have some filters from way back which I think I bought from Schott (I
have no connection with the company!).

Try going to

www.google.com

and searching on

optical glass filters

or something similar. Schott comes up as do many others of course.

Good luck

Gareth

Gareth Morgan MPhil MSc FIBMS,
Institute for Microbiology,
Pathology and Immunology (IMPI), H5,
Karolinska Institutet,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

OBS! Bes�ksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92

From daemon Thu May 16 03:05:30 2002

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----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 15, 2002 2:23 AM

I have seen today Panasonic Lumix DMC-LC5A with Leica lenses and looks
also very good.
3,9 Mega pixels
just for AUS dollars 1775
Ricardo
Keep care and be of good cheer

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
(title) 84 duke of Siebenl�gner

websites:
http://www.coleoptera.org. and
http://www.egroups.com/group/coleoptera

University of Sydney
The Wentworth Bldg., B 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ICQ: 13610107

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).

From daemon Thu May 16 07:51:42 2002

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Hi Alan,
At 100g without batteries, I would certainly NOT mount such a camera
without the added support of a mini-tripod, or macro-stand.

Here are some sites that have adapters:

http://www.marksimmons.org/closeup/adapter/adapter.html

http://www.marksimmons.org/closeup/adapter/adapter.html [for angle
viewing???]

http://www.mir.com.my/rb/photography/hardwares/classics/olympusom1n2/om1/om1
manual/index5.htm
[an old Olympus OM1 site with a 'massive' camera stand to isolate it
from scope]

http://www.lensadapter.com/optical_article.htm [here's a guy who has your
problem]
http://www.lensadapter.com/default.htm [looks like just what you need!!!!]

These last two were the best finds of the exercise. Hope it helps.

Fred

} ----------
} From: Alan E. Davis
} Sent: Wednesday, May 15, 2002 6:26 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: More digiterata: Microscope attachment for Olympus E20N?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Just another posting about digital microscope adaptors. I am looking for
} informatio leading to the ordering and purchase of an adaptor for the
} Olympus E20N digital camera.
}
} At the college lab where I will be teaching as an adjunct this summer, a
} new toy has been purchased: an Olympus E20N digital camera. Some
} discussion has focused on purchase of a microscope attachment. The
} attachment that has been recommended by one of the staff attaches to the
} 62mm filter ring threads of the camera. I have two scopes that I use in
} teaching, each of them w/ c. 45. eyetube inclination angles; there is no
} trinocular tube available. I fear the forces of attaching a camera to a
} scope by the filter ring at such an angle would be unhealthy for the
} camera. The camera is a hefty one.
}
} Alan Davis
}
} --
} Alan E. Davis
} Science Department
} Marianas High School
} PMB 30, Box 10006,
} Saipan, MP 96950
} Northern Mariana Islands
} adavis-at-saipan.com
}
}
} "An inviscid theory of flow renders the screw useless, but the need
} for one non-existent."
} ---Lord Raleigh(aka John William Strutt),or else
} his son, Jr., who was also a scientist.
}
}

From daemon Thu May 16 12:21:57 2002

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The Army Research Laboratory, Adelphi MD (http://www.arl.army.mil) is about
to advertise a position for an experienced Ph.D. level TEM specialist. We
are looking for someone with experience and publications in the TEM of
semiconductor materials/devices. Experience with or interest in Auger
electron spectroscopy, MOCVD, wide bandgap materials, and FIB sample
preparation would be beneficial. We have a JEOL 2010 and a JEOL 2010F with
EDX. Applicants MUST BE US CITIZENS. ARL is an equal opportunity
employer. Please e-mail resumes directly to me, although, this will not
constitute an official application for the position which must be done by
responding to the official posting.

Sincerely,
Matthew Ervin, Ph.D.
MErvin-at-ARL.Army.mil

M/S: AMSRL-SE-RL
US Army Research Laboratory
2800 Powder Mill Road
Adelphi, MD 20783-1197

Disclaimer: The opinions and views expressed above are those of the author
and do not necessarily represent those of the U.S. Army Research Laboratory
or any other government agency

From daemon Thu May 16 13:26:25 2002

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The camera you refer to is the Sony DSC-F707. It has 5MP resolution, a
Zeiss zoom lens, USB interface and uses Memory Stick media for storage.
http://www.sonystyle.com/digitalimaging/P_Feature_F707.shtml

} I recently saw a new Sony camera with a large lens that can focus } down to
about one inch and claims to have better low-light
} performance than most digitals.... ... it is hard to miss, since } it looks
funny with a tiny digital camera body attached to a
} large, complex optical lens.

The Nikon Coolpix 5000 is also a 5MP camera with a Nikon zoom lens, .75
inch minimum focus, and accepts compact flash media. It will also shoot
320x240 Quicktime movies(the Sony also has a "video" mode). Image quality
is excellent. In addition to standard exposure controls, the CP5000 also
has adjustments for contrast, noise reduction, sharpening and saturation.
An electronic release is also available to reduce potential vibration from
depressing the shutter or to do interval timing. Microscope couplers are
readily available for the 5000 to fit most microscopes.
http://www.nikonusa.com/usa_product/product.jsp?cat=1&grp=2&productNr=25501
or we have a PDF available.

} Can anyone suggest other digital cameras in the same price range } that
will do microscopy work (compound and dissecting)(macro and
} micro), good outside and indoor photos and can be used to photograph
electrophoresis gels.

George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com

From daemon Thu May 16 14:52:56 2002

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Hello all,
would anybody have any thoughts on the Retiga 1300 Monochromatic
cooled CCD camera (for fluorescent microscopy) a and its price: CAN$
12,300.00 (CAN$ = US$ 0.63).

Thank you for your help,
Sincerely,

Sarka Lhotak

Hamilton Regional Cancer Centre
McMaster University
Hamilton, Ontario, Canada

From daemon Thu May 16 15:18:13 2002

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Hi All,
We have a EMITech K850 Critical Point Dryer and a new tank of
Research Grade CO2, and we have problems.
The runs have all of a sudden become VERY inconsistent.

Ambient temp in the Lab is around 18oC
After cooling the chamber to 5oC and opening the Inlet valve, the
pressure rises to 750psi at which is will remain for at least an hour with
all valves closed.
With the Inlet valve open, however, and waiting for liquid CO2 to
show in the sight glass, the temperature begins to rise and does so to
around 10oC at which point the fluid begins to rise in the glass to about
1/8 of the 1cm diameter.
If I re-cool the chamber to 5oC, the fluid disappears.
If I wait for up to 3 minutes, one of two things happens.
1. the fluid reappears and rises to 1/2 the sight window,
or
2. I see nothing until I release the pressure and for a
brief period note the fluid level show at the very top of the sight glass
and fall and disappear very rapidly.
After the first run, which I cannot control, I cannot merely recyle
the unit. I have to wait an hour to demonstrate another unsatisfactory
test.

If anyone can help me ferret out this poltergeist, I would be most
appreciative. I must say that I am convinced that I am dealing with a
daemon from the PChem lab.

I attached my Polaron CPD, which is much simpler in design and saw
the same initial 750 psi but NO fluid accumulation after watching for 10min.

Hoping that it is NOT that terrible tank with the broken siphon (again after
all these years),

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

From daemon Fri May 17 02:40:15 2002

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Look at :

http://www.microscopy-uk.org.uk/mag/artaug01/vrcoolpix.html

It's a nice description of a home made adaptor to fit a Nikon CoolPix on a
microscope, using the tripod thread insted of the filter ring threads of
the camera. I don't know if the Olympus E20N has such a thread, but it
gives some idee how to build something which works well and is not
expensive.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Mat�riaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Thu, 16 May 2002, Alan E. Davis wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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}
}
} Just another posting about digital microscope adaptors. I am looking
} for informatio leading to the ordering and purchase of an adaptor for
} the Olympus E20N digital camera.
}
} At the college lab where I will be teaching as an adjunct this summer,
} a new toy has been purchased: an Olympus E20N digital camera. Some
} discussion has focused on purchase of a microscope attachment. The
} attachment that has been recommended by one of the staff attaches to
} the 62mm filter ring threads of the camera. I have two scopes that I
} use in teaching, each of them w/ c. 45. eyetube inclination angles;
} there is no trinocular tube available. I fear the forces of attaching
} a camera to a scope by the filter ring at such an angle would be
} unhealthy for the camera. The camera is a hefty one.
}
} Alan Davis
}
} --
} Alan E. Davis
} Science Department
} Marianas High School
} PMB 30, Box 10006,
} Saipan, MP 96950
} Northern Mariana Islands
} adavis-at-saipan.com
}
}
} "An inviscid theory of flow renders the screw useless, but the need
} for one non-existent."
} ---Lord Raleigh(aka John William Strutt),or else
} his son, Jr., who was also a scientist.
}

From daemon Fri May 17 09:07:20 2002

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I don't think you will see the difference between 143 and 138 resolution
there are many factors that come into play as previously mentioned. Another
one is are you replacing the window on your detector also. There are an
assortment of different windows that have different energy line curves and
strengths. Some may be more suited to your application that is if you are
doing something so touchy that 138 resolution to 143 resolution is going to
come into play. Why did you need to replace your crystal? Are you replacing
your F. E. T. also, as that will effect the detector resolution as well.

Daniel Connors

From daemon Fri May 17 10:21:30 2002

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Sorry for intruding with a non-microscopy question, but I am looking
for feedback on a nationwide ISP. My present ISP has dropped their
East Coast dial-ups, and I need access when I travel.

I am looking for the least-intrusive (i.e. not AOL) nationwide ISP.
Any suggestions?

Please reply privately unless you have some information you would
like the entire list to see.

Thanks

pbg

From daemon Fri May 17 11:05:03 2002

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Hi All,

For those who order CO2-w/siphon tube for critical point drying.

Do you order:

the 'Research Grade' CO2 (-at-$300) or

the standard grade CO2 (-at-$7)?

Thanks,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

From daemon Fri May 17 11:45:23 2002

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The Sony F707, Nikon 5000, Olympus E20N and Minolta Dimage 7 all use the
same 5 Mega Pixel Sony CCD. Reviews, and test photos comparing resolution,
color response and S/N characteristics can be found at this web site:
http://www.dpreview.com/
The way I read it, the Sony F707 beats them all quite handily. In addition,
the lens is internal focusing, so the end of the camera doesn't move during
focusing or poweron/poweroff. In addition, the body with the display on it
tilts so that it can be viewed conveniently while mounted vertically. This
would be a good to adapt to a microscope, but I do not know if anyone has
built a commercially available adapter.

John Mardinly

-----Original Message-----
} From: George Laing [mailto:scisales-at-ngscorp.com]
Sent: Thursday, May 16, 2002 11:18 AM
To: MSA Microscopy Society of America

The camera you refer to is the Sony DSC-F707. It has 5MP
resolution, a
Zeiss zoom lens, USB interface and uses Memory Stick media for storage.
http://www.sonystyle.com/digitalimaging/P_Feature_F707.shtml

} I recently saw a new Sony camera with a large lens that can focus } down to
about one inch and claims to have better low-light
} performance than most digitals.... ... it is hard to miss, since } it looks
funny with a tiny digital camera body attached to a
} large, complex optical lens.

The Nikon Coolpix 5000 is also a 5MP camera with a Nikon zoom lens,
.75
inch minimum focus, and accepts compact flash media. It will also shoot
320x240 Quicktime movies(the Sony also has a "video" mode). Image quality
is excellent. In addition to standard exposure controls, the CP5000 also
has adjustments for contrast, noise reduction, sharpening and saturation.
An electronic release is also available to reduce potential vibration from
depressing the shutter or to do interval timing. Microscope couplers are
readily available for the 5000 to fit most microscopes.
http://www.nikonusa.com/usa_product/product.jsp?cat=1&grp=2&productNr=25501
or we have a PDF available.

} Can anyone suggest other digital cameras in the same price range } that
will do microscopy work (compound and dissecting)(macro and
} micro), good outside and indoor photos and can be used to photograph
electrophoresis gels.

George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com

From daemon Fri May 17 15:26:12 2002

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Hi!

I am trying to interface a PC to an Oxford eXL/Lemas system on a
JEOL 6300 SEM in order to get external control of the stage. Has anyone
tried this and/or do you have any suggestions for getting this to work?

Connections:

(1) eXL (main Oxford control computer using Genie OS) {-{6 pin
"LaserBus" & 15 pin stage sync.}-} Lemas (stage motherboard and motor
drivers)

(2) Lemas {-{15 pin cable}-} joystick & keypad

(3) Lemas {-} stepper motors & limit

Thanks!
*************************************************************************
Frank E. Jones email: jonesfe-at-darkwing.uoregon.edu
Department of Physics URL: http://darkwing.uoregon.edu/~jonesfe
Office: 189b Klamath
Lonergan Lab (Chemistry)
University of Oregon
Office Phone: (541)346-0977
-------------------------------------------------------------------------

From daemon Fri May 17 21:29:46 2002

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Eric & Co.;

The I.R., in my opinion, seems the likely suspect for the hotspot. I took
an optical microscope and deliberately converted it into an IR.. reflected
light microscope to image through crystalline compounds like GaAs. What I
did was put a filter in the path that blocked the visible light but was a
bandpass to I.R. The I.R. naturally is generated by the illuminator. Of
course the human eye cannot see into the infra-red but a CCD camera can and
does and that is used for imaging and recording vis-a-vis a monitor. I
removed the I.R. blocking filter from a B/W camera [just in front of the CCD
array to make this work] I also have what you described as a "hot spot." I
am trying to diffuse this with a filter that will still transmit I.R. In
your case you may need an I.R. blocking filter as you stated. I am looking
at Edmund Scientific for a low budget solution.

Please let us know if, or when, you find a solution.

Regards,

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Eric Johnston [mailto:ericdj-at-seas.upenn.edu]
Sent: Wednesday, May 15, 2002 11:09 AM
To: Microscopy-at-sparc5.microscopy.com

Hi all

wow, I got a lot of really useful replies.

The majority suggest that there is some kind of reflection on one or more
of the lens surfaces that's causing the hot spot, especially from the
camera adapter (in my case, the adapter has no lens so that's out).

The other suggestion was that the hot spot is due to IR, which CCDs can be
especially sensitive to. I need to find out if there is an IR blocker in
light path or on the camera itself.

Thanks for all the helpful suggestions. I'll keep you updated.

Eric

From daemon Sat May 18 10:39:58 2002

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Greetings,

I have a dual gun Gatan ion thinner that runs a 1500W oil diffusion pump.

The rules at Brooklyn College require no heat down the drain and water
conservation.
Thus I need a recirculating chiller. Current lab chillers are $4,000 and
up
however the pump doesn't require constant temperature so I don't see why
an expensive controller, etc is necessary.

Any ideas for a recirculating chiller that is less expensive?

Also any US government labs with a surplus recirculator. It can be
donated to an American University.

Thanks,
Gordon Nord
Environmental Science Institute
Brooklyn College, Brooklyn, NY
gnord-at-mindspring.com

From daemon Sat May 18 14:11:58 2002

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I hope you don't mind my intrusion onto your list sever. It was
suggested
by the business office that I post my question here.

I am an amateur microscopist that has dabbling on and off since grade
school,
exploring the world of the miniature, and interesting views of the
ordinary.

I have recently become more serious about this "hobby", and have adapted
a
digital camera to the photo tube of an optical microscope.

I am hoping to find some type of amateur microscopy association(s) that
would
help enhance my work and enjoyment. My web search only turned up a
group in
the UK, and I was hoping to find something "closer to home" (Wisconsin,
USA).

Thank you kindly;

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

From daemon Sat May 18 17:43:04 2002

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Gordon,
I prefer the Neslab CFT 25 chiller, either digital or analog. The work
just fine for a small DP and are very reliable. Check out LABx or a
reseller like Bid Service. They usually have some in stock, around $1K.

Gary M. Easton
Scanners Corporation
Third Party SEM Service

No financial interest in any of the above companies(except mine of course).

----- Original Message -----
} From: "Gordon Nord" {gnord-at-mindspring.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, May 18, 2002 11:26 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kchamusco-at-mail.ifas.ufl.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
May 18, 2002 at 16:01:58
---------------------------------------------------------------------------

Email: kchamusco-at-mail.ifas.ufl.edu
Name: karen chamusco

Organization: U of FL

Education: Graduate College

Location: Gainesville FL

Question: I'm sorry if I should be usinf the list serve but this is
the only page I could find to ask a question. I have been working
with corn pollen and trying to embed it in LR White resin for
immunolabeling in TEM. I thought I had this problem solved, but,
apparently I don't. When I get my pollen through the EtOH series up
to 95% it looks very good, but when I try to embed it in the resin it
collapses. I have tried putting it in resin/EtOH at 20% steps and
directly embedding it in 100% resin. The 20% stepwise series seems to
be the least destructive and I can get partially collapsed pollen
sometimes. I really need to be able to see inside a "fluffy" whole,
uncollapsed pollen grain. Is there anyone who can help with this
before I pull out the rest of my hair? If necessary to know, I fix
in 4% paraformaldehyde + 0.5% gluteraldehyde + 4% sucrose + 0.3%
tween {to get the samples to sink}. I vacuum infiltrate for 5 minutes
then I leave them in the fridge overnight. I then rinse with PBS and
send through a 10% EtOH series{anything greater causes the pollen to
collapse}. Then the rest is as I described above. Thanks, Karen

---------------------------------------------------------------------------

From daemon Sun May 19 02:29:37 2002

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If you use a CCD or CMOS camera you need an IR filter some where. Most color
cameras have some filtering but not all. Many monochrome cameras do not have
any filtering.

IR light will be out of focus as well as causing hot spots. Silicone in a
CCD camera is sensitive into IR light to 1000 nm. With the sensitivity at
800 nm being high enough to cause some problems. A curve with and with out
IR blocking filters are given here:
http://www.edmundoptics.com/techsupport/DisplayArticle.cfm?articleid=266

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger
} From: "Peter Tomic" {PTomic-at-anadigics.com}
: Eric & Co.;
:
: The I.R., in my opinion, seems the likely suspect for the hotspot. I took
: an optical microscope and deliberately converted it into an IR.. reflected
: light microscope to image through crystalline compounds like GaAs. What I
: did was put a filter in the path that blocked the visible light but was a
: bandpass to I.R. The I.R. naturally is generated by the illuminator. Of
: course the human eye cannot see into the infra-red but a CCD camera can
and
: does and that is used for imaging and recording vis-a-vis a monitor. I
: removed the I.R. blocking filter from a B/W camera [just in front of the
CCD
: array to make this work] I also have what you described as a "hot spot."
I
: am trying to diffuse this with a filter that will still transmit I.R. In
: your case you may need an I.R. blocking filter as you stated. I am
looking
: at Edmund Scientific for a low budget solution.
:
: Please let us know if, or when, you find a solution.
:
: Regards,
:
: Peter Tomic
: Anadigics, Inc.
:
:
:
: } From: Eric Johnston [mailto:ericdj-at-seas.upenn.edu]
: :
: wow, I got a lot of really useful replies.
:
: The majority suggest that there is some kind of reflection on one or more
: of the lens surfaces that's causing the hot spot, especially from the
: camera adapter (in my case, the adapter has no lens so that's out).
:
: The other suggestion was that the hot spot is due to IR, which CCDs can be
: especially sensitive to. I need to find out if there is an IR blocker in
: light path or on the camera itself.
:
: Thanks for all the helpful suggestions. I'll keep you updated.
:
: Eric
:
:

From daemon Sun May 19 06:17:50 2002

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In about 6 months time I will have to move my microscopes into a newly
re-furbished two-storey building. The chillers for the FEI CM120 and
Hitachi 4700 may have to be located on the roof, at about 8.5 metres
from ground level. I would like to hear from anyone who has
considered this, and would particularly value the first-hand
experiences of anyone who has already tried it. Are the pumps in
standard chillers capable of dealing with an 8-metre head?

Chris

From daemon Sun May 19 20:02:40 2002

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Hi Chris,

We had a Nesslab unit with a pump in our 4th floor fan loft.
Our CM12 and SEM were located on the first floor.

I installed pressure gauges at the inlet and outlet lines where they enter
the labs. Typical readings on the first floor were 55 PSI on the inlet
(from Nesslab) and 15 PSI on the return outlet after going through our
scopes. This 15 psi 'back pressure' was simply the pressure of the water
standing in the pipe that was 3-4 floors or about 30-35 feet high.
The first floor inlet pressure was then 40 PSI + 15 PSI or 55 PSI to the
scopes. This is only a net pressure difference of 40 PSI across your scopes.

Upstairs on the outlet side of the Neslab, the pressure gauge at the unit
in the fan loft was 40 psi. The 'suction' or inlet at the Nesslab read
zero. The buffer tank is open to air. YOUR chiller will not even notice
any pressure difference but you will get a loss of 15 PSI to your
instruments as shown above.

The net effect was that we had an extra 15 PSI on the whole system on the
first floor even when the pump was turned OFF. Don't remove any lines on
instruments without shutting both the return and the supply lines OFF.

This pressure worked out to 5 PSI per floor for the three floors above us.
I think you will see about 15 PSI back pressure on your instruments on the
first floor.

We were forced to install a system that runs off our HVAC chilled water
through two heat exchangers in series. It has automatic city water
chilling for backup. The temperature of city water is not constant
throughout the year.
The last heat exchanger and pump is on the first floor and eliminates the
back pressure problem. You might consider a heat exchanger and pump on
your floor to eliminate the back preesure. My CM 12 can handle much higher
pressures. Your does too I believe.

As for your problems:
Run DI or distilled water on a CM series scope. Never use chloramine-T
because of chloride pit corrosion of SS fittings. Check with Philips
service.

Make sure you have a DI water pump to keep the recirc' cooling tank topped
off. If you have a leak on the first floor, then you will empty any open
buffer tank 'upstairs' fast.

We had a small pump tied into a big DI water tank to deliver enough water
to buy us some time to react to a leak problem. It automatically topped
off the buffer tank. Also if I drained any water out on the first floor to
change the water, it pumped in the required replacement amount without
going upstairs.

You need someone in the group to go around and check that all plastic
connections are water tight and secure to avoid draining the buffer tank
over a weekend, say. Use brass tubing inserts and hose clamps. Make sure
all plastic solvent welds are secure.

Use good rubber pressure hose where possible.

You might need a remote temperature light and readout in the first floor
hallway to tell someone when there is a problem. This wasn't that
effective an alarm system for us. People just walked by without noticing a
problem. SO.......

I also have in my lab a small cheap audible temp alarm that has the probe
fitted under the chilled water insulation. It is set to go off if any
minute temperature rise occurs. I bought this from McMaster-Carr Hardware
supply. If it even chirps, I go check the system light and temp readout
down the hall, check all pressure readings, and go up to the fan loft and
watch the freon compressor cycle. This alarm can also alert you to a
partial loss in freon before a sudden total system failure and it allows
one to shut down all instruments safely and to notify maintainence of a
slight loss in cooling. I highly recommend this alarm.

We originally installed L-type copper 1 inch manifold lines from the fan
loft to the first floor and the labs. We tore all that out and now use
CPVC dark gray plastic pipe, I believe. This was done to avoid green
copper carbonate build up in the RETURN--RETURN lines after diffusion pumps.

Hope this helps.

Paul Beauregard
Senior Research Associate

}
} In about 6 months time I will have to move my microscopes into a newly
} re-furbished two-storey building. The chillers for the FEI CM120 and
} Hitachi 4700 may have to be located on the roof, at about 8.5 metres
} from ground level. I would like to hear from anyone who has
} considered this, and would particularly value the first-hand
} experiences of anyone who has already tried it. Are the pumps in
} standard chillers capable of dealing with an 8-metre head?
}
} Chris
}
}
}
}

From daemon Mon May 20 07:25:53 2002

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Morning All,
For those who are interested in the responses to my last question,
here's the dope.

I received 9 responses from VERY reputable sources.
NONE used what I called 'Research Grade' CO2
NONE paid more than ~$40, and most paid {$20 / tank
Most ordered what they called "bone dry" CO2
One used a hot water pipe heating strip for 1hr before use
to insure access to entire liquid charge in tank.

Appreciation to all for your help and advice.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

} ----------
} From: Monson, Frederick C.
} Sent: Friday, May 17, 2002 11:58 AM
} To: 'List-Microscopy'
} Subject: CPD Question Two
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Hi All,
}
}
} For those who order CO2-w/siphon tube for critical point drying.
}
} Do you order:
}
} the 'Research Grade' CO2 (-at-$300) or
}
} the standard grade CO2 (-at-$7)?
}
} Thanks,
}
} Fred Monson
}
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} West Chester University
} South Church Street and Rosedale
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}

From daemon Mon May 20 08:33:07 2002

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At 11:58 AM -0400 5/17/02, Monson, Frederick C. wrote:
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From daemon Mon May 20 10:27:15 2002

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Sounds like you have a flow rate problem something is constricting the
flow of CO2 into the chamber. The chamber will get warm when you fill it (the
CO2 in the syphon tank is at 18 C vs the chamber at 5 C). When you cool
the chamber the CPD uses vented high pressure CO2 to cool the chamber
(PV=nRT), but since there is a restriction in the flow from the CO2 supply
tank, the CO2 back flows from the chamber dropping the CO2 liquid level.

I have found CO2 flow problems caused by:

- low CO2 supply tank

- tank valve must be openned fully (minus one 1/2 turn) - don't use the
tank valve as a regulator.

- clogged filters. I have worked with different setups with various line
filters (particle, oil, moisture, etc.) which get clogged and need replacing. I
have also found some CPDs have (scintered?) metal mesh filters for particle
filtration and these get clogged too.

- Worked with one instrument in which the internal plumbing lines were
just too small and if you flushed or cooled too fast the liquid level in the
chamber would drop and you had to be very carful and slow about things.

Hope it helps!

(Oh we use the cheap standard CO2)

On 16 May 2002, at 16:11, Monson, Frederick C. wrote:

}
} Hi All,
} We have a EMITech K850 Critical Point Dryer and a new tank of
} Research Grade CO2, and we have problems.
} The runs have all of a sudden become VERY inconsistent.
}
} Ambient temp in the Lab is around 18oC
} After cooling the chamber to 5oC and opening the Inlet valve, the
} pressure rises to 750psi at which is will remain for at least an hour with
} all valves closed.
} With the Inlet valve open, however, and waiting for liquid CO2 to
} show in the sight glass, the temperature begins to rise and does so to
} around 10oC at which point the fluid begins to rise in the glass to about
} 1/8 of the 1cm diameter.
} If I re-cool the chamber to 5oC, the fluid disappears.
} If I wait for up to 3 minutes, one of two things happens.
} 1. the fluid reappears and rises to 1/2 the sight window,
} or
} 2. I see nothing until I release the pressure and for a
} brief period note the fluid level show at the very top of the sight glass
} and fall and disappear very rapidly.
} After the first run, which I cannot control, I cannot merely recyle
} the unit. I have to wait an hour to demonstrate another unsatisfactory
} test.
}
} If anyone can help me ferret out this poltergeist, I would be most
} appreciative. I must say that I am convinced that I am dealing with a
} daemon from the PChem lab.

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If it is simply a matter of recirculation, it shouldn't be a problem even
it there was a difference of several stories. Whether the pump is on the
roof or in the EM room, since the inlet and outlet are at approximately the
same height, there should be no net elevation head to be concerned about.

You would have the extra elevation head generating increased pressure in
the line at its low point, but that it would only amount to about 15 psi or
one bar for that height difference.

There may be some issue due to the length of the loop, but I will leave
that to someone with more experience in that area.

Warren

At 12:11 PM 5/19/02 +0100, you wrote:
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From daemon Mon May 20 13:47:14 2002

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Thanks to all who tried to help me with my head problem, I mean my microscope head problem ;-)

The guys from the company whose camera we bought came down and fixed the problem we were having with the camera. Now it looks like we're needing to buy a new LM, one that has the proper Trinoc head to handle our camera.

Again thanks to all who assisted me.

Looking at tiny things on big microscopes,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

From daemon Mon May 20 14:37:23 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

University of Connecticut
Institute of Materials Science

Postdoctoral Positions

Two post-doctoral position are available immediately within the
Institute of Materials Science at the University of Connecticut. Both
positions require extensive hands-on experience in transmission electron
microscopy, scanning electron microscopy, and x-ray diffraction. These
positions are one-year appointments, with possible renewal for a second
year.

The first position is for work on characterization and synthesis of
ceramic coatings, thin films, and nanocomposites. Experience in the
areas of materials chemistry and synthesis/processing is highly
desirable. Contact: Prof. Nitin Padture at: nitin.padture-at-uconn.edu

The second position is for a study of the character and motion of
interfacial defects in titanium aluminide alloys. An individual with
prior experience in the use of HREM and analysis of interfacial
crystallography would be preferred. Contact Prof. Mark Aindow at
m.aindow-at-uconn.edu

To apply, please send a complete resume, together with a list of
publications and contact details for 3 referees to the appropriate
contact named above. Screening of applications will begin on June 1, and
will continue until the positions are filled. We encourage applications
from under-represented groups, including minorities, women and people
with disabilities.

--
*********************************************************

Mark Aindow, Associate Professor,
Department of Metallurgy and Materials Engineering
Institute of Materials Science,
97 North Eagleville Road, Unit 3136
University of Connecticut, Storrs,
CT 06269-3136, USA

Tel: +1 (860) 486-2644
FAX: +1 (860) 486-4745
Email: m.aindow-at-uconn.edu

**********************************************************

From daemon Mon May 20 15:09:47 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are closing a Neurobiology EM lab and have an extra working Zeiss 902
available. If you are interested look at the official MSA website and the
Surplus Listing.

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954

From daemon Tue May 21 07:27:01 2002

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Chris,
We have a Hitachi H7500, and a 3000-N. We use an air cooled
Haskaris chiller for both, and located the chiller in a well ventilated
rooftop service enclosure, approx. 12 metres above the EM room. In 2 years
we have had no problems with this set-up. Saves water, eliminates noise and
heat near the microscopes, plus the space savings.

David O'Neil
Institute for Marine Biosciences
National Research Council of Canada
1411 Oxford St.
Halifax, NS B3H 3Z1
ph. 902-426-8258
fax 902-426-9413
david.o'neil-at-nrc.ca
http://www.imb.nrc.ca/micros_e.html

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: May 19, 2002 8:11 AM
To: microscopy-at-sparc5.microscopy.com

In about 6 months time I will have to move my microscopes into a newly
re-furbished two-storey building. The chillers for the FEI CM120 and
Hitachi 4700 may have to be located on the roof, at about 8.5 metres
from ground level. I would like to hear from anyone who has
considered this, and would particularly value the first-hand
experiences of anyone who has already tried it. Are the pumps in
standard chillers capable of dealing with an 8-metre head?

Chris

From daemon Tue May 21 12:54:22 2002

Contents Retrieved from Microscopy Listserver Archives
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From daemon Tue May 21 12:54:27 2002

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I have a customer who needs to determine whether
his glass and crystal objects were polished by hand
or with machines. The objects are too big to fit
in microscope, so I am looking for advice how to
prepare replicas of their surfaces which will not
leave marks on the objects.

Thank you,

Vladimir Dusevich

From daemon Tue May 21 13:06:08 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Karen,
Thought I remembered something from history about pollen processing,
but......specifics are lost. I think I found a relevant reference.
----------------------------------------------------------------- Immunogold electron microscopy of soluble proteins: localization of Bet v I
major allergen in ultra-thin sections of birch pollen after anhydrous
fixation techniques M Grote Institute of Medical Physics, Munster University, Germany. To localize the highly water-soluble major allergen Bet v I in ultra- thin
sections of birch pollen, pollen grains were cracked, air-dried, and
processed for electron microscopy using one of the following preparation
techniques: fixation in aqueous p-formaldehyde + cetylpyridinium chloride;
fixation in p-formaldehyde vapor; fixation in benzoquinone vapor; inert
dehydration; or no fixation. Afterwards the pollen grains were embedded in
Lowicryl K4M resin at low temperature. Ultra-thin sections were cut and
incubated with a monoclonal antibody against Bet v I, followed by a
gold-labeled secondary antibody. In some experiments, commercial rabbit IgG
antibodies against birth pollen allergens were also used, followed by
incubation with the protein A- gold complex. Bet v I could be localized only
after vapor fixation and in the inert dehydrated specimens. Best
preservation of ultrastructure and antigenicity was obtained after
p-formaldehyde vapor fixation. Bet v I antibody binding sites were detected
only in the cytoplasmic matrix of the pollen grain, never in the pollen
wall. Commercial rabbit antibodies bound to cytoplasm and wall of all
prepared specimens, even after aqueous fixation. This might be explained by
the assumption that these antibodies recognize a variety of antigenic and
allergenic structures, not all of which are so highly soluble as Bet v I. Volume 39, Issue 10, pp. 1395-1401, 10/01/1991 Copyright � 1991 by The Histochemical Society

Hope this gives you another starting point.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

} ----------
} From: kchamusco-at-mail.ifas.ufl.edu
} Sent: Saturday, May 18, 2002 9:56 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: corn pollen
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (kchamusco-at-mail.ifas.ufl.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
} May 18, 2002 at 16:01:58
} --------------------------------------------------------------------------
} -
}
} Email: kchamusco-at-mail.ifas.ufl.edu
} Name: karen chamusco
}
} Organization: U of FL
}
} Education: Graduate College
}
} Location: Gainesville FL
}
} Question: I'm sorry if I should be usinf the list serve but this is
} the only page I could find to ask a question. I have been working
} with corn pollen and trying to embed it in LR White resin for
} immunolabeling in TEM. I thought I had this problem solved, but,
} apparently I don't. When I get my pollen through the EtOH series up
} to 95% it looks very good, but when I try to embed it in the resin it
} collapses. I have tried putting it in resin/EtOH at 20% steps and
} directly embedding it in 100% resin. The 20% stepwise series seems to
} be the least destructive and I can get partially collapsed pollen
} sometimes. I really need to be able to see inside a "fluffy" whole,
} uncollapsed pollen grain. Is there anyone who can help with this
} before I pull out the rest of my hair? If necessary to know, I fix
} in 4% paraformaldehyde + 0.5% gluteraldehyde + 4% sucrose + 0.3%
} tween {to get the samples to sink}. I vacuum infiltrate for 5 minutes
} then I leave them in the fridge overnight. I then rinse with PBS and
} send through a 10% EtOH series{anything greater causes the pollen to
} collapse}. Then the rest is as I described above. Thanks, Karen
}
} --------------------------------------------------------------------------
} -
}
}

From daemon Tue May 21 17:14:23 2002

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Vladimir -

A conservator or scientists at the Corning Museum of Glass can advise you.
Their main number is 800.732.6845.

James Martin
Conservation Scientist

----- Original Message -----
} From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
To: "microscopylistserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, May 21, 2002 1:45 PM

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Vladimir Dusevich wrote:
============================================================
I have a customer who needs to determine whether his glass and crystal
objects were polished by hand or with machines. The objects are too big to
fit in microscope, so I am looking for advice how to prepare replicas of
their surfaces which will not leave marks on the objects.
============================================================
We have done something very similar to this, the first time going back into
the mid-1970's. We used what was then the prototype of the SPI Supplies :
"Wet Replica Kit" as shown on URL
http://www.2spi.com/catalog/spec_prep/wet_rep_kits.shtml

I realize this is not a wet surface but the kit was originally developed for
the replication of a "wet" surface such as a plant leaf or even human skin.

Since it is a silicone, so long as the components are properly mixed, there
should be no residue left behind on the surface. And the lift-off is easy
enough that one does not generally need any kind of metal tools (e.g. a
scalpel blade or tweezers) to help get the left off started.

We have also tried the cellulose acetate replica approach, see URL
http://www.2spi.com/catalog/submat/cellulose-acetate-replicating-tape-sheets
.shtml
but we have had greater difficulty getting the cellulose acetate to lift off
without the use of some kind of sharp instrument to start the lift-off.

Another advantage of the Wet Replica kit is that the replica itself can be
replicated so that what goes into the SEM is something that spatially, looks
just like the actual surface.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================

From daemon Wed May 22 00:56:22 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

This question is on behalf of our OM operator.

We have a Leitz DMRBE OM and have just purchased a Leica DC 300F digital
camera to use with it. The engineer recommended we get a x0.63 C-mount
adaptor to use with the camera, particularly for fluorescence work "to
concentrate the low light levels".

When using the camera with the x0.63 adaptor we get a dark shading on one
side of the image(approximately 25% of the width of the image). This
shading is independent of the camera alignment (ie you can rotate the camera
and the shading doesn't rotate (although the image does)) and it also
appears to be independent of the alignment of the adaptor. (ie you can
rotate the adaptor and the shading doesn't rotate)

If you use a plain tube adaptor or a 1x adaptor there is no evidence of this
shading. We have also tried the camera on other microscopes with identical
results (ie shading only when using the x0.63 adaptor). We have also tried
two different x0.63 adaptors and the shading was the same.

In the manual it mentions that some vignetting MAY occur using the x0.63
adaptor. Is this what we are seeing? The manual doesn't offer a solution
other than not using the x0.63 adaptor. Does anyone have any ideas how we
may be able to get around this problem other than not using the adaptor?

Thank you very much in advance.

Colin Veitch

Instrumentation Scientist
Late Stage Innovation Group
CSIRO Textile and Fibre Technology
PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au
Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Fax: +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may not
copy, distribute or take any action in reliance on it. If you have received
this message in error, please telephone CSIRO Textile and Fibre Technology
on +61 3 5246 4000.

From daemon Wed May 22 02:53:07 2002

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Dear Vladimir,

I would like to offer you the opportunity to put your samples into our large
chamber SEM. There is enough space for glass and crystal objects up to a
volume of 1 cubic meter and 500 pounds of weight. Further more we can
analyze the chemical composition of the glass surfaces by EDS.

Especially the analytic capabilities might be an advantage in distinguishing
the production method.

Best regards
Martin Klein

VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen/Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: mklein-at-visitec-em.de
WWW: http://www.visitec-em.de
++++ Home of the world's largest SEM ++++

-----Urspr�ngliche Nachricht-----
Von: Dusevich, Vladimir [mailto:DusevichV-at-umkc.edu]
Gesendet: Dienstag, 21. Mai 2002 19:45
An: microscopylistserver
Betreff: Replicas of glass surface

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have a customer who needs to determine whether
his glass and crystal objects were polished by hand
or with machines. The objects are too big to fit
in microscope, so I am looking for advice how to
prepare replicas of their surfaces which will not
leave marks on the objects.

Thank you,

Vladimir Dusevich

From daemon Wed May 22 06:19:21 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone know of the cold mounting resisn called 'Scandiplast' and
who supplies it?

Thanks in advance,
Steven

--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk

From daemon Wed May 22 08:34:30 2002

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I have a user with the same basic need, only looking at marks on
ancient ceramics. These are museum pieces and cannot be altered. He
makes casts with latex or wax, usually latex.

Phil

} I have a customer who needs to determine whether
} his glass and crystal objects were polished by hand
} or with machines. The objects are too big to fit
} in microscope, so I am looking for advice how to
} prepare replicas of their surfaces which will not
} leave marks on the objects.
}
} Thank you,
}
} Vladimir Dusevich

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

From daemon Wed May 22 09:15:12 2002

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Colin,

You use a .63x tube normally to adapt the field of view of the camera to the
field of view through the binoculars. I am not sure what that has to do with
"concentrating the light". And the physical reason behind that is, that the
camera uses a chip that is bigger than the standard TV chips. Some
vignetting (darker edges) is indeed normal when you use such a setup, but
you may be able to minimize it by carfully adjusting all the parameters of
the microscope and illumination path. In particular, it should be symmetric.
Since you are using a digital camera, you can correct the images by using a
shading or background correction. That's a software feature, and you may
have to check if it is supported. If not, you can take a background image by
removing the sample and taking a picture. then take a normal picture with
your sample and divide or subtract the background image from the regular
image.

mike

} } } } } } } } } } WE HAVE MOVED { { { { { { { { {
please make a note of the new address below

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: "Colin.Veitch-at-csiro.au"-at-sparc5.microscopy.com
[mailto:"Colin.Veitch-at-csiro.au"-at-sparc5.microscopy.com]
Sent: Tuesday, May 21, 2002 11:46 PM
To: microscopy-at-sparc5.microscopy.com

Hi All,

This question is on behalf of our OM operator.

We have a Leitz DMRBE OM and have just purchased a Leica DC 300F digital
camera to use with it. The engineer recommended we get a x0.63 C-mount
adaptor to use with the camera, particularly for fluorescence work "to
concentrate the low light levels".

When using the camera with the x0.63 adaptor we get a dark shading on one
side of the image(approximately 25% of the width of the image). This
shading is independent of the camera alignment (ie you can rotate the camera
and the shading doesn't rotate (although the image does)) and it also
appears to be independent of the alignment of the adaptor. (ie you can
rotate the adaptor and the shading doesn't rotate)

If you use a plain tube adaptor or a 1x adaptor there is no evidence of this
shading. We have also tried the camera on other microscopes with identical
results (ie shading only when using the x0.63 adaptor). We have also tried
two different x0.63 adaptors and the shading was the same.

In the manual it mentions that some vignetting MAY occur using the x0.63
adaptor. Is this what we are seeing? The manual doesn't offer a solution
other than not using the x0.63 adaptor. Does anyone have any ideas how we
may be able to get around this problem other than not using the adaptor?

Thank you very much in advance.

Colin Veitch

Instrumentation Scientist
Late Stage Innovation Group
CSIRO Textile and Fibre Technology
PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au
Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Fax: +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may not
copy, distribute or take any action in reliance on it. If you have received
this message in error, please telephone CSIRO Textile and Fibre Technology
on +61 3 5246 4000.

From daemon Wed May 22 09:19:15 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everybody - what is the best method for prep on a dried out moth?
Just Au coat or if you have low vacuum mode available - go with that
uncoated?
Thanks
Barb

From daemon Wed May 22 10:39:43 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How difficult will it be to get up on the roof to check the chillers for
water level? I check mine weekly.
Will the water lines freeze in the winter?
Joyce Craig
Chicago State University

From daemon Wed May 22 10:45:03 2002

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Regarding a Toluidine Blue stain solution:

1% Toluidine Blue O
1% Sodium Tetraborate Dodecahydrate
30% Ethanol
in DDI

I've been using this stain for years on Epon-Araldite sections and Durcupan
sections with no problems in getting satisfactory staining. When I have
30-50mL remaining, I always make a new batch so it's ready the next time I
need it , but this time I forgot. I made a new solution and had to use it
right away. I stained my sections as usual, but the color of the sections
was bright blue instead of the more purple-blue we desire. Thinking that
I'd made a mistake in preparing the solution, I made another batch and
decided to wait overnight to try it, but again the sections were bright
blue.

I was never aware of the need to do this, but does this solution need to
"ripen" for a week or so before using? I can't think of any other reason.
The quality of our water hasn't changed, and everything else is the same as
I've always used.

Thank you for any help you can give me.

Jaclynn M. Lett, Research Technician
Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu
314-977-0257

From daemon Wed May 22 10:51:47 2002

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You could try dental impression materials, available from any dental
supplier. There are various types, I think the best type for your purpose
would be one of the silicone-based ones. This would give a negative
impression, of course. To get a positive I use epoxy resins: place the
silicone impression at the bottom of a small container and pour a de-bubbled
resin to make a layer 2 or 3 mm thick. After curing, the resin impression is
indistinguishable from the original in the SEM. Hope this helps.

Lesley Weston.

on 21/05/2002 10:45 AM, Dusevich, Vladimir at DusevichV-at-umkc.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a customer who needs to determine whether
} his glass and crystal objects were polished by hand
} or with machines. The objects are too big to fit
} in microscope, so I am looking for advice how to
} prepare replicas of their surfaces which will not
} leave marks on the objects.
}
} Thank you,
}
} Vladimir Dusevich
}

From daemon Wed May 22 12:34:20 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Butterlies and moths, and other scaly insects such as some beetles
etc. can be a right pain.
The overlapping scales make it almost impossible to put down a
continuous coating.
Even well-coated specimens may give problems with charging. If the
whole moth is the objective,
then the underside needs to be coated as well as the top. I
contributed a tip for doing that with hard objects like seeds etc.
some time ago,
but the method I described then will damage a moth. The simplest
thing
to do with a moth is to coat the underside before mounting. Once
mounted coat the top. The more angles you coat from the better, so
rotary coating will help, even in a sputter coater. Carbon seems to
reach the parts other coating materials cannot reach, so a
preliminary
rotary/tilting coating with carbon before sputtering may improve the
overall conductivity. I used to do that routinely, but it has gone
out
of favour, mostly for good reasons.
Low vac mode may help somewhat, but not if you need high res images
of
scale details. Keep the kV and beam current as low as possible, use
short exposures if you have enough signal to do so. In the worst-case
scenario collecting an image by frame-integration at tv rate may be
the only way, but image quality is rarely very good. Try a little
specimen bias if available. Use a FEG SEM if available. Use the lower
detector if there is a choice.

Chris

} ----- Original Message -----
} From: "barbara maloney" {bmalon01-at-fiu.edu}
} To: "'microscopy-at-msa.microscopy.com'"
} {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, May 22, 2002 3:10 PM
} Subject: SEM technique
}
}
}
} --------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
} ---.
} }
} }
} } Hi everybody - what is the best method for prep on a dried out
moth?
} } Just Au coat or if you have low vacuum mode available - go with
that
} } uncoated?
} } Thanks
} } Barb
} }
} }
}

From daemon Wed May 22 13:23:39 2002

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If specimen permits, I always coat it. Images are much
better for coated specimens.

Vladimir

} -----Original Message-----
} From: barbara maloney [mailto:bmalon01-at-fiu.edu]
} Sent: Wednesday, May 22, 2002 9:10 AM
} To: 'microscopy-at-msa.microscopy.com'
} Subject: SEM technique
}
}
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}
} Hi everybody - what is the best method for prep on a dried out moth?
} Just Au coat or if you have low vacuum mode available - go with that
} uncoated?
} Thanks
} Barb
}
}
}

From daemon Wed May 22 14:46:29 2002

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Would any one please share with use your user fees of your microscopic
facility including charges for imaging? I would appreciate your help. We
are in the process of establishing such charges and hope to find
willingness of your part to share this information.

Many thanks.

Anne-Marie Brun
Research Associate
Mol. Biology and Genetics
UNT HSC
Fort Worth, Texas

From daemon Wed May 22 15:33:48 2002

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Hi all

I have an update to my hotspot problem that unfortunately does not include
any solutions.

First of all, we don't get the problem on the transmitted side of the
scope. The cheap halogen lamp seems to work fine. Also, we have another
Nikon TE300 microscope in the lab with the same Coolsnap HQ camera and we
get exactly the same thing. That seems rather suspicious.

I think I can see this effect with a regular video camera if I threshold
the image.

Here is a list of things we have done so far. None of them have produced
any appreciable change.

1) 10 degree holographic diffuser in place of one of the neutral density
filters right after the lamp.
2) IR filter in the collector lens.
3) IR filter before the camera.
4) aligned and misaligned the lamp in every possible way.
6) focused and defocused the lamp in every possible way.
7) changed out filter block.
8) reduced the exposure time of the camera down to 1 ms.

So it doesn't seem to be IR.

I'll let you know if we figure it out.

Eric

Eric Johnston
Design Engineer and Research Specialist
Department of Bioengineering
University of Pennsylvania
5170 Vagelos
220 South 33rd Street
Philadelphia, PA 19104
215-573-6696
215-573-7616
(F) 215-573-2071
ericdj-at-seas.upenn.edu

From daemon Wed May 22 15:44:15 2002

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Mr. Klein,

You should be aware, as should anyone else who might attempt this, that certain glass and rock crystal artifacts are prone to catastrophic fracturing when subjected to pressure or humidity changes - crystal in the first case and glass in the second.

Rock crystal artifacts are often subject to considerable residual stress and must be protected from sudden temperature or pressure excursions. Certain glasses, many from archaeological origins but also some from the 19th Century, are unstable with respect to water and may have undergone partial hydrolysis with a resulting incorporation of hydrous silica in places formerly occupied by alkali-oxygen-silica links. Such glasses are sometimes visually unchanged but may shatter to powder when subjected to dehydrating conditions as under the vacuum of a SEM chamber.

While the advantages of your large chamber instrument are well known, the examination of replicas is safer unless a great deal is known about the behavior of the specific object.

John Twilley
Art Conservation Scientist

On Wed, 22 May 2002 09:39:39 +0200 Martin Klein {Visitec-at-t-online.de} wrote:

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Dear Vladimir,

I would like to offer you the opportunity to put your samples into our large
chamber SEM. There is enough space for glass and crystal objects up to a
volume of 1 cubic meter and 500 pounds of weight. Further more we can
analyze the chemical composition of the glass surfaces by EDS.

Especially the analytic capabilities might be an advantage in distinguishing
the production method.

Best regards
Martin Klein

VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen/Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: mklein-at-visitec-em.de
WWW: http://www.visitec-em.de
++++ Home of the world's largest SEM ++++

-----Urspr�ngliche Nachricht-----
Von: Dusevich, Vladimir [mailto:DusevichV-at-umkc.edu]
Gesendet: Dienstag, 21. Mai 2002 19:45
An: microscopylistserver
Betreff: Replicas of glass surface

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I have a customer who needs to determine whether
his glass and crystal objects were polished by hand
or with machines. The objects are too big to fit
in microscope, so I am looking for advice how to
prepare replicas of their surfaces which will not
leave marks on the objects.

Thank you,

Vladimir Dusevich

From daemon Wed May 22 16:12:26 2002

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Dear Jaclynn,

Try perhaps to control the pH of your Toluidine Blue stain solution.

If I remember well, Toluidine Blue Stain is metachromatic when it is acidic
(no real difference between pH 4 and 7) but it is no more (only blue) when
basic (for example at pH 8.2, at least for plant tissue embeded in JB4).

Hope it helps !

Chris Wuethrich
Beth Israel Deaconess Medical Center
330 brookline AVE
Boston, MA 02215
cwuethri-at-caregroup.harvard.edu

From daemon Wed May 22 18:39:21 2002

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In a message dated 05/21/2002 11:02:03 PM US Mountain Standard Time,
Colin.Veitch-at-csiro.au-at-sparc5.microscopy.com writes:

{ { When using the camera with the x0.63 adaptor we get a dark shading on one
side of the image(approximately 25% of the width of the image). This
shading is independent of the camera alignment (ie you can rotate the camera
and the shading doesn't rotate (although the image does)) and it also
appears to be independent of the alignment of the adaptor. (ie you can
rotate the adaptor and the shading doesn't rotate) } }

Colin,

I know the Leica DC500 has a "shading correction" routine you can use to even
out the illumination, but I don't know if the 300F has the same routine. We
had a similar problem with a 0.63X adapter on a DC500, and running the
"shading correction" took care of the problem.

If the camera can do this (check the manual), you will see a "shading"
function on the left side of the screen where you set the image size, auto
contrast, etc. Proceed as follows:

Click on "shading" and first set the black level: shut off all light to the
camera and click on "black level." After this, click on "shading" again and
then set the white level by sending light to the camera and making sure there
is no specimen or tissue in the field of view. Then, grab a "white level."
You may have to play with the "brightness" slider at the top of the window
until the camera accepts the bright level. It will inform you if the
illumination is too dark or too light.

Also, when you set the white level you will have to assign a name to it...you
can call it "10x," or "40x," or whatever, for the objective you are using, or
maybe "brightfield" or another name of your choice.

If you have access to this routine on the 300F, it should take care of
moderate differences in illumination such as hot spots and shadows.

If the shading is severe, it may be too much for the correction factor to
handle. If this is the case, you will probably either need to use a 1.0X
adapter or a Leica c-mount "vario-zoom" adapter, which allows you to set the
magnification from about 0.3 up to 1.3 or so, much like a zoom lens for a
35mm camera.

Good luck, let us know how things work out!

Cheers,

Bob Chiovetti

From daemon Wed May 22 21:23:28 2002

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Hi everybody - thanks to those who responded. Wanted to let you know,
we ended (before I received your responses) coating 367 Ang of Au for 2
min. Prior to coating we did view at low vacuum with no coat - got an
image, of course not as nice as when we coated at high vacuum. Just
used a large carbon tab on viewed ventral side of moth - really nice
images. Thanks gang.
Barb
P.S. I will try your suggestions. Thanks again

From daemon Wed May 22 22:42:55 2002

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barbara maloney wrote:

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}
} Hi everybody - what is the best method for prep on a dried out moth?
} Just Au coat or if you have low vacuum mode available - go with that
} uncoated?
} Thanks
} Barb

Hi Barb
I routinely deal with insects especially hairy and scaly specimens. What I
have found is,
firstly when you mount the specimen - I always mount the whole animal on a
pin - use carbon or silver impregnated glue to stick the moth onto the pin
or directly onto a stub.
Secondly if mounting directly onto an aluminum stub, I use a double sided
carbon sticky tab on the stub then apply the glue to the tab then the
specimen. This ensures good conductivity.
Thirdly when coating be careful not the coat too heavily with gold - you
will loose some fine detail on the scales, antennae etc. Coat the specimen
several times (short coating times) in differing positions - this will
ensure total coverage. With the specimen on a pin it makes coating the whole
specimen a lot easier.
Once coated I then recoat the pin with the carbon glue. This makes the pin
nonconductive and once in the SEM you do not see the pin. Thus the moth
looks like it is sitting in space.

We use a LEO 435VP SEM in high vacuum mode to do this work. The way I avoid
charging (if it is charging much at all) is to use the Robinson Backscatter
Detector. The images derived are fantastic. Quite contrasty!!!
Another thing is I often mix the Backscatter signal with the Secondary
signal. This allows me to enhance the background, bring out the edges, seta
etc that appear to be flattened in the backscatter image.

I hope this helps

Sue

--
Sue Lindsay

SEM Laboratory Manager
Scanning Electron Microscope Unit
The Australian Museum
6 College st
Sydney, 2010
NSW, Australia

ph 02 9320 6198
fax 02 9320 6059
Email suelind-at-austmus.gov.au

From daemon Wed May 22 22:42:56 2002

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From daemon Thu May 23 07:57:39 2002

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A student working in the lab is interested in using TEM to examine
thickness of the chitinous exoskeleton and the interface between muscle and
skeletal attachments in some insects (Heteroptera). Does someone have
experience in embedding and ultramicrotomy of this type of material? I
expect we will have some problems with the harder parts pulling out of the
resin. Are there any tricks we should know about? Any special fixation
steps or suggestions? Thanks.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2242
University of Connecticut
Storrs, CT 06269-2242
Phone: 860-486-3588
Fax: 860-486-6369

From daemon Thu May 23 08:22:14 2002

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} Morning Anne-Marie,
} When I need such information, I try searching Google for {core
} facility charges} . Pages and pages.
}
} Regards,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} West Chester University
} South Church Street and Rosedale
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
}
} ----------
} From: Anne-Marie Brun
} Sent: Wednesday, May 22, 2002 3:38 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Microscopic user fees
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Would any one please share with use your user fees of your microscopic
} facility including charges for imaging? I would appreciate your help. We
} are in the process of establishing such charges and hope to find
} willingness of your part to share this information.
}
} Many thanks.
}
} Anne-Marie Brun
} Research Associate
} Mol. Biology and Genetics
} UNT HSC
} Fort Worth, Texas
}
}
}

From daemon Thu May 23 08:30:53 2002

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Hi,
Here is the way we do the TB:
1% TB in H2O
Saturate with Na Borate (Na2B4O7.10H2O) (25g for 500ml)
Leave 24h at 4 C
Filter with Whatman No.1, 2 times
Leave at 4 C for a week before use.
Filter with a syringe filter before each use.
Keep at 4 C. Protect from light.
Hope it helps.
Emmanuelle.

Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620

From daemon Thu May 23 08:33:52 2002

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Hi Ann-Marie,
You can go to our website for the fees for all of the core facilities
here at Weill-Cornell:
www.med.cornell.edu/research/cores

specifically the EM core can be founds at the above address plus:
/core1/sop.html
and our oprical core facility is at: /core3/sop.html
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

From daemon Thu May 23 08:44:35 2002

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Hi All,
Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
please provide brief description and some citations.
Thanks!

John Shields
EM Lab
Univ. of Georgia
Athens, GA 30602

From daemon Thu May 23 08:54:50 2002

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Morning Jaclynn,

Well, let's see. What I know is that to preserve metachromasia -
Toluidine blue is one of the thazine dyes that is known for metachromatic
staining - one is admonished NOT to use ethanol - I use tertiary
butanol(TBA) - to dehydrate/differentiate a section. Of course, in
semi-thin staining, most procedures do not suggest anything but rinsing
with water followed by drying before mounting - if that is how you prepare
the semi-thin for viewing.

I have never put ethanol in my Toluidine blue stains. With the
above in mind as well as the normal semi-thin staining protocols with which
I am familiar, if I did add ethanol it would be to restrict/prevent
metachromasia in the preparation.

Why aging? I can't give an all-knowing response, but if the ethanol
slows some coordination of the buffer with the dye, then that would be an
explanation.

I presume your recipe is something like this:

1g TolBlue
1g Sodium borate
30ml 100% ethanol
70ml water(d.i.)

If so then we are on the same page. Perhaps what you need to do is
add the ethanol, if you continue to use it, sometime (hours?) after you make
up the aqueous dye-buffer solution.

My recipe comes from a small Lab Manual by: Giuseppe
Millonig(1976), Laboratory Manual of Biologic electron Microscopy, Mario
Saviolo - Editore, Vercelli C.P. 182, Italy.

Method 3(Millonig Ref: Richardson, K.C., Jarret, L, Finke,
E.H.(1960), Embedding in epoxy resins for ultrathin sectioning in electron
microscopy, Stain. tech., 35: 313)

0.5g sodium borate
50ml water

0.5g Toluidine Blue [O] - C.I. 52040) - You should check -
Lillie, R.D.(Ed.)(1977), H.J. Conn's Biological Stains(9th Ed.), Williams
and Wilkins, Baltimore - for information on contrast between old and new
dyes with this name.

Above sequence as given in Millonig.

I often use it fresh, and I can't ever remember a problem,
even though, like you, I tend to make it in advance of need, at least by a
day.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

} ----------
} From: Jaclynn Lett
} Sent: Wednesday, May 22, 2002 11:32 AM
} To: Microscopy Listserver (E-mail)
} Subject: Toluidine Blue question
}
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}
} Regarding a Toluidine Blue stain solution:
}
} 1% Toluidine Blue O
} 1% Sodium Tetraborate Dodecahydrate
} 30% Ethanol
} in DDI
}
} I've been using this stain for years on Epon-Araldite sections and
} Durcupan
} sections with no problems in getting satisfactory staining. When I have
} 30-50mL remaining, I always make a new batch so it's ready the next time I
} need it , but this time I forgot. I made a new solution and had to use it
} right away. I stained my sections as usual, but the color of the sections
} was bright blue instead of the more purple-blue we desire. Thinking that
} I'd made a mistake in preparing the solution, I made another batch and
} decided to wait overnight to try it, but again the sections were bright
} blue.
}
} I was never aware of the need to do this, but does this solution need to
} "ripen" for a week or so before using? I can't think of any other reason.
} The quality of our water hasn't changed, and everything else is the same
} as
} I've always used.
}
} Thank you for any help you can give me.
}
} Jaclynn M. Lett, Research Technician
} Electron Microscopy Core Facility
} Fay and Carl Simons Center for Biology of Hearing and Deafness
} Central Institute for the Deaf
} 4560 Clayton Ave.
} St. Louis, MO 63110
}
} jlett-at-cid.wustl.edu
} 314-977-0257
}
}

From daemon Thu May 23 09:01:52 2002

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Dear Listers,
I am asking for your input once again. I am looking for a
source to obtain
"a diamond knife that can be installed onto a compound microscope
like a lens so that it can be used to cut a tiny circle on a plastic
membrane on a glass slide. One would look for a particular cell or
tissue with the desired properties using a regular lens (40X or
100X), and then switch to the knife, which is just a little off
center, and turn the knife around the central axis to score the
around the sample. A little buffer would then float the cut sample,
which can then be picked up by a TEM grid for observation using TEM."
Rosemary

--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html

From daemon Thu May 23 09:11:56 2002

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Dear Barbara,

I would coat the dried moth with gold or gold/palladium and it should be
fine.

Good Luck,

Jackie Garfield
LifeCell Corp.

From daemon Thu May 23 09:31:16 2002

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It sounds like X-ray photoelectron spectroscopy (aka ESCA).

You use a monochromatic x-ray beam (usually Al or Mg target) to irradiate a
surface. The x-rays create photoelectrons which have characteristic
energies. Because the photoelectrons are low energy, the only ones to
escape come from the top 1-5nm of the sample. Hence the technique is a
surface measurement technique. By careful measurement of the electron
energies, the bonding state of the element can be determined, helping to
identify the chemical compound.

You might check out the book by Czenderna (or Czanderna) and articles by
M.P. Seah.

At 09:36 AM 5/23/02 -0400, you wrote:
} ------------------------------------------------------------------------
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Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.

From daemon Thu May 23 10:17:13 2002

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Sender: "Hendrik O. Colijn" {colijn.1-at-osu.edu}

} It sounds like X-ray photoelectron spectroscopy (aka ESCA).

} You might check out the book by Czenderna (or Czanderna) and articles
} by
} M.P. Seah.

And UK Surface Analysis Forum website at www.uksaf.org

Keith

----
Dr. Keith R. Hallam University of Bristol, Interface Analysis Centre,
Oldbury House, 121, St. Michael's Hill, Bristol, BS2 8BS, England
Telephone: + 44 (0)117 925 5666 | E-mail: k.r.hallam-at-bristol.ac.uk
Facsimile: + 44 (0)117 925 5646 | URL: http://eis.bris.ac.uk/~phkrh/

From daemon Thu May 23 11:05:22 2002

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Hi everybody - help!! I have a 2mm thick silicate glass sample - with
laser ablations in the diameter of ~50 um. We are expecting depth
measurement of ~70um. We want to view side of conical crater formed by
the laser, however after coating with 367 ang of Au, using SEI and BEI
not really seeing what we expected. On light scope, able to view the
conical crater. Any suggestions to what we can do to get a SEI image?
Thanks so much
Barb

From daemon Thu May 23 11:10:07 2002

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Vickerman's surface analysis book gives a pretty good overview of XPS,
AES, etc. The title is something like Surface Analysis: the Principal
Techniques.

Eve

On Thu, 23 May 2002 jshields-at-cb.uga.edu-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Hi All,
} Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
} please provide brief description and some citations.
} Thanks!
}
} John Shields
} EM Lab
} Univ. of Georgia
} Athens, GA 30602
}
}

From daemon Thu May 23 11:13:13 2002

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John,

Vickerman's book on surface analysis gives a pretty good overview of XPS,
AES, etc. The title is something like Surface Analysis: the Principal
Techniques.

Eve

On Thu, 23 May 2002 jshields-at-cb.uga.edu-at-sparc5.microscopy.com wrote:

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}
} Hi All,
} Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
} please provide brief description and some citations.
} Thanks!
}
} John Shields
} EM Lab
} Univ. of Georgia
} Athens, GA 30602
}
}

From daemon Thu May 23 12:37:30 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Looks like a typo to me. Likely should be X-ray Photoelectron Spectroscopy
(XPS) aka Electron Spectrscopy for Chemical Analysis (ESCA)

Excellent surface analysis technique for determining elemental
concentrations of surfaces (top few atomic layers) and some information on
chemical bonding environment. Many applications in polymer science,
microelectronic manufacturing, corrosion, and more. (I'm running some
samples this afternoon!)

For introductions explore the following web sites:
http://www.uksaf.org/home.html
http://www.uwo.ca/ssw/

There are many books; here are a few from my shelf:
Practical Surface Analysis, 2nd ed., Vol 1--Auger and X-ray Photoelectron
Spectroscopy. Edited by D. Briggs & M. P. Seah
Modern ESCA: The Principles and Practice of X-Ray Photoelectron
Spectroscopy. Tery L. Barr
Surface Analysis: The Principle Techniques. Edited by John C. Vickerman

Hope this helps!

Jim Passmore
Sr. Analytical Chemist
Cryovac Division, Sealed Air Corporation
Duncan, SC

"jshields-at-cb.u
ga.edu" To: Microscopy-at-sparc5.microscopy.com
cc:
05-23-02 09:36 Subject: x-ray photoctron?
AM

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hi All,
Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
please provide brief description and some citations.
Thanks!

John Shields
EM Lab
Univ. of Georgia
Athens, GA 30602

From daemon Thu May 23 13:43:20 2002

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Dear Rosemary
It's out of topic, but it seems to me, people use lasers for such
purpose. You could direct laser using the same lens you are using for
observation and use it as a 'laser blade'. I kind of pessimistic how you
could use proposed tool with x100 lens: distance between lens and sample is
too small. I know tnat our Medical School do have commercial apparatus for
such purpose. I believe it's Olympus (very expensive). Sergey

At 10:47 AM 5/23/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From daemon Thu May 23 13:44:08 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is it possible to buy a digital camera that can be used as a camera and by
using an adaptor also be mounted on any light microscope for use in
photomicrography? I've been asked by my dept. chairman to gather
information. His goal, a departmental camera for everyone to use. As
regards the light photomicrography it would be bright field and
fluorescence. Any information will be appreciated, I'm a complete newbie
in this digital stuff.

Tom Bargar
CEMRF-Univ. of Neb. Med. Ctr.
tbargar-at-unmc.edu
402-559-7347

From daemon Thu May 23 14:10:48 2002

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Hi John,
Sounds like a typo that should read x-ray photoelectron
spectroscopy. A web search for "XPS" or "ESCA" (electron spectroscopy for
chemical analysis) should give you all the info you need.
In brief, monochromatic soft x-rays (typically Mgka 1253.6eV or AlKa
1486.6eV) are used to photo-emit electrons from core atomic levels of
elements in a solid sample in high vacuum. The photoelectrons are energy
analyzed to give a spectrum which uniquely defines the elements present in
the sample (except H or He).
Subtle shifts in peak position for each element gives information on
the bonding of that element within the sample (hence "chemical analysis").
Another major advantage of the technique is that it is extremely
surface sensitive. Only the top ~100 Angstroms of the sample are analyzed
because although the x-rays penetrate much deeper the mean free path of
electrons of energy ~100eV-1486eV in a solid is typically {50A so only
electrons in the near-surface region can escape the material without
scattering.

All the Best,

Steve

Steve Buckingham
Excellatron Solid State LLC.
1640 Roswell St, Suite J.
Smyrna, GA 30080
Ph: 770 438 2201
Fax: 617 812 5920
sbuckingham-at-excellatron.com {mailto:sbuckingham-at-excellatron.com}

-----Original Message-----
} From: "jshields-at-cb.uga.edu"-at-sparc5.microscopy.com
[mailto:"jshields-at-cb.uga.edu"-at-sparc5.microscopy.com]
Sent: Thursday, May 23, 2002 9:37 AM
To: Microscopy-at-sparc5.microscopy.com

Hi All,
Anyone ever heard of x-ray "photoctron" spectroscopy? If so,
please provide brief description and some citations.
Thanks!

John Shields
EM Lab
Univ. of Georgia
Athens, GA 30602

From daemon Thu May 23 14:30:12 2002

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SPECIAL MEETING:

Thursday, June 6th

"Research At Longwood Gardens -
In search of the perfect horticultural display"

Jim Harbage,
Plant Physiologist/Propagator, Longwood Gardens

To be held at:
Longwood Gardens, Kennett Square PA

Please see the Announcements Section for important information regarding
electronic copies of this notice!
Meeting Details

Location: LONGWOOD GARDENS, Kennett Square PA
Schedule: 4:30 - 5:30: Guided tour of including the
production greenhouse and (hopefully) the pest management lab.
6:00 Buffet dinner at the Terrace Restaurant
7:00 Talk
Directions: Can be found at http://www.longwoodgardens.org/
Cost: Entrance to the park is $12. We are also collecting $10 to
help defray the cost of dinner.
For those who want to take the tour, we will be gathering at the
Conservatory main entrance at 4:30 (I know that's too early for some, but
that's the best we can do). For everyone else, we will have a table set up
at the Terrace Restaurant.
The talk, at 7:00, will be be presented at the Terrace Restaurant.
Reservations
Reservations required. We have been asked to give Longwood Gardens an idea
of how many people will attend. Please let me know if you will be attending
the tour or just the dinner and talk.
By E-Mail (preferred): Send your name and affiliation to
jrreffner-at-rohmhaas.com
By Phone: Call John Reffner, 215-619-5283
DEADLINE Monday, June 3th. I will confirm all e-mail reservations

About the Speaker

Jim Harbage is Plant Physiologist/Propagator and I work in the Research
Division at Longwood Gardens, Inc. He has been there about 2.5 years.
Prior to that he was an Associate Professor of Horticulture at South Dakota
State University for 6 years. He hold a BSc and MSc from University of
Maryland - College Park and a Ph.D from University of Wisconsin - Madison.
All are with majors in horticulture. His primary focus areas at Longwood
include conduct plant trials and plant improvement programs, optimize plant
culture, utilize tissue culture methods for propagation and virus
elimination.

Other Announcements
IMPORTANT NOTICE REGARDING EMAIL:
It is important that PSM has your email address. We are considering
switching our meeting notifications to email ONLY. This would save the
society some money and significantly reduce the work of the executive
committee. Also, and most importantly, we could communicate more often and
give members more advanced notice of upcoming events.

EASTERN ANALYTICAL SYMPOSIUM:IMPORTANT ANNOUNCEMENT
The Eastern Analytical Symposium will return to its Somerset, New Jersey
location starting with the 2002 Meeting
The Symposium Dates will be November 18-21, 2002 Garden State Exhibit
Center, Somerset, New Jersey

Philadelphia Society for Microscopy Executive Council, 2001
President Past President President-Elect Secretary-Treasurer and
Newsletter Editor Corporate Liaison
Robert Carlton Pat Connelly Robert Carlton John Reffner Open
(610)- 313-1360 (215)- 898-7145 (610)- 313-1360 (215) 619-5283

Robert A. Carlton
Elan Drug Delivery, Inc.
3500 Horizon Drive
King of Prussia, PA, 19406
610-313-1360
robert.carlton-at-elan.com

********************************************************************
This communication and any files transmitted with it
may contain information that is confidential, privileged
and exempt from disclosure under applicable law.
It is intended solely for the use of the individual or entity
to which it is addressed. If you are not the intended
recipient, you are hereby notified that any use,
dissemination or copying of this communication is strictly
prohibited. If you have received this communication in
error, please notify the sender. Thank you for your co-operation.
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From daemon Thu May 23 16:13:05 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all of you who responded to my email. You are a real help.
My new question now to you is: if you charge all that money, how fast is
your turn around time of samples? Would you please mention how many
people work in the lab, who actually do the work.
Thanks
Anne-Marie Brun-Zinkernagel

From daemon Thu May 23 16:38:09 2002

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Dear Listers;
Does anyone have suggestions on how to fit or couple a retail (like a Sony
or Cannon) digital video camera to a microscope? Are there any commercial
products to do this? I've been asked by a Prof who'd like to (on the cheap)
capture the activities of microscopic marine invertebrates for later editing
and compiling on his desktop computer. He has a Sony digital camcorder and
Apple G4 system. I'd really appreciate your feedback.

Thanks;
Richard Harris
Laboratory Supervisor
Imaging and Analysis
Department of Zoology
University of Western Ontario
London ON
CANADA N6A 5B7
(519) 661-2111 ext 86780
(519) 661-2014 Fax

From daemon Thu May 23 22:16:27 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In a message dated 05/23/2002 7:07:02 AM US Mountain Standard Time,
rw9-at-psu.edu writes:

{ { "a diamond knife that can be installed onto a compound microscope
like a lens so that it can be used to cut a tiny circle on a plastic
membrane on a glass slide. One would look for a particular cell or
tissue with the desired properties using a regular lens (40X or
100X), and then switch to the knife, which is just a little off
center, and turn the knife around the central axis to score the
around the sample. A little buffer would then float the cut sample,
which can then be picked up by a TEM grid for observation using TEM."
} }

Rosemary,

Most microscope manufacturers can provide a diamond slide marker or diamond
scribe that's used to mark slides...I suppose you could use it to scribe a
plastic section and then float off the scribed area.

The only difficulty would be a lack of control of the circle's size. You
could only scribe one size of circle, whatever the scribe comes equipped to
do. This would make it rather difficult to excise single cells if they are
too close together.

You might want to check out the Eppendorf website. They make a micro-chisel
that's designed for this type of thing. It mounts on the end of a
micromanipulator and also has a micropipette for flushing the chiseled-out
cell and collecting the material. Go to {www.eppendorf.com} and follow the
links: Products -} Cell Technology -} Microdissector.

You can also check with the microscope manufacturers for specialized
instruments that can do the same thing: laser microdissection (Leica), laser
capture (Arcturus), laser catapulting (Zeiss/PALM), etc. Very cool
technology, but also very pricey.

It would probably be quite difficult to pick up the excised single cell in
plastic and transfer to an EM grid, but it might be worth a try. I suppose
you could use either the Leica laser microdissection system of the PALM unit,
since they are non-contact methods, and collect the excised specimen onto a
drop of buffer.

The Arcturus probably wouldn't be suitable, since it depends on heating a
transfer film to make it sticky and then adhering the tissue or specimen to
the film.

Maybe there's a core lab or a research pathology lab in your area that has
one of the above instruments??

Good luck!

Cheers,

Bob Chiovetti

From daemon Thu May 23 23:28:27 2002

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Rosemary,

Could a dual beam FIB be used to cut your "tiny circle" ? How thick is the
plastic?

-Ed

Sergey Ryazantsev {sryazant-at-ucla.edu} on 05/23/2002 11:52:43 AM

To: Rosemary Walsh {rw9-at-psu.edu} , microscopy-at-sparc5.microscopy.com
cc:

Dear Rosemary
It's out of topic, but it seems to me, people use lasers for such
purpose. You could direct laser using the same lens you are using for
observation and use it as a 'laser blade'. I kind of pessimistic how you
could use proposed tool with x100 lens: distance between lens and sample is
too small. I know tnat our Medical School do have commercial apparatus for
such purpose. I believe it's Olympus (very expensive). Sergey

At 10:47 AM 5/23/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From daemon Fri May 24 00:28:41 2002

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Dear Barbara

If you still run into charging problems on your moth try a thin Carbon coat
under high vacuum prior to a AuPd coat. Rotary coating in the high vacuum
will be preferred during the carbon evaporation.
If you are fortunate to have a ESEM AuPd coating will not be needed. We had
some nice fun here and were rewarded with interesting pics ;-)

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gabarone
Botswana
{ {...OLE_Obj...} }

Phone : +267 355 2426
Mobile: +267 718 96 729
Fax : +267 585 097
e-mail : coetzees-at-mopipi.ub.bw {mailto:coetzees-at-mopipi.ub.bw}

From daemon Fri May 24 06:34:18 2002

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Hi,

I'm wondering whether our objective aperture is correctly positioned (or the
column
correctly aligned).
If I focus a diffusely scattering specimen at the eucentric height and then
switch to diffraction
mode with a relatively parallel beam (the beam covers the entire screen at a
magnification of
about 30K) I can't get a sharp (minimal) central spot and a sharp image of
the aperture
at the same time.
Is there something wrong with our microscope or with my idea of electron
optics?

sincerely,

Philip Koeck
Svdertvrns Hvgskola and
Karolinska Institutet
Dept. of Bioscience at Novum
S-14157 Huddinge
Sweden
phone: +46-8-6089186
fax: +46-8-6089290
http://www.biosci.ki.se/em

The phrase 'We have always done things this way.'
is as much a reason to change as a reason not to.
- Dartwill Aquila
_______________________________________

From daemon Fri May 24 06:57:02 2002

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Richard, Diagnostics Instrument, Inc. has a nice catalog of couplers for
all sorts of microscopes and all sorts of cameras. info-at-diaginc.com and
www.diaginc.com. I have no commercial interest in the company but have used
their products.

Don Marshall
RELION Industries
cathodoluminescence microscopy
mass spectroscopy
electron beam technology
PO Box 12
Bedford, MA 01730

781-275-4695 (Phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

http://www.excitingelectrons.com

In a message dated 05/24/2002 4:11:10 AM Eastern Daylight Time,
rjharris-at-uwo.ca writes:

{ { Subj: Fitting a digital video camera to a microscope
Date: 05/24/2002 4:11:10 AM Eastern Daylight Time
From: rjharris-at-uwo.ca (Richard Harris)
Reply-to: rjharris-at-uwo.ca
To:

(Microscopy Listserver)

Dear Listers;
Does anyone have suggestions on how to fit or couple a retail (like a Sony
or Cannon) digital video camera to a microscope? Are there any commercial
products to do this? I've been asked by a Prof who'd like to (on the cheap)
capture the activities of microscopic marine invertebrates for later editing
and compiling on his desktop computer. He has a Sony digital camcorder and
Apple G4 system. I'd really appreciate your feedback.

Thanks;
Richard Harris

From daemon Fri May 24 07:53:40 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard and Tom;

(Tom, I'm assuming that you have a sub $1,000 digital camera in mind, as
opposed to a full blown SLR type digital.)

I have coupled both a Sony still, and a Sony Camcorder to a light
microscope. There is a commercial adapter that can be found at:

http://www.lensadapter.com

In looking at this online, I noticed that there were no optics, and that
it was just an adapter tube. As a quick test, I took a number of
cameras from both work and home, and just held them to the eyepiece, and
lo, there was a useable image.

For the small and light still camera I have, I machined an adapter with
a slight press fit over the camera lens barrel, and a slip fit over the
microscope eyepiece. This works great. The machining needs to be close
on the fits, as any "wobble" results in vignetting of the image.

For the heavier still cameras and camcorders, I've used a tripod to
support the weight of the camera, but am working on an easier to adjust
stand (X, Y, Z adjustments) to align and support the camera.

The only real issue I've run into is focusing. For the most part, the
cameras auto focus works well for the camera itself, but in my case the
focus of the viewing tube and camera tube are not always exactly the
same. Trying to focus via the digital cameras LCD screen is less than
optimal, as the resolution is too low. You kind of have to go for the
"least blurry" image.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Richard Harris [mailto:rjharris-at-uwo.ca]
Sent: Thursday, May 23, 2002 4:39 PM

Dear Listers;
Does anyone have suggestions on how to fit or couple a retail (like a
Sony
or Cannon) digital video camera to a microscope? Are there any
commercial
products to do this? I've been asked by a Prof who'd like to (on the
cheap)
capture the activities of microscopic marine invertebrates for later
editing
and compiling on his desktop computer. He has a Sony digital camcorder
and
Apple G4 system. I'd really appreciate your feedback.

Thanks;
Richard Harris
Laboratory Supervisor
Imaging and Analysis
Department of Zoology
University of Western Ontario
London ON
CANADA N6A 5B7
(519) 661-2111 ext 86780
(519) 661-2014 Fax

-----Original Message-----
} From: "tbargar-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"tbargar-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Thursday, May 23, 2002 1:02 PM

Is it possible to buy a digital camera that can be used as a camera and
by
using an adaptor also be mounted on any light microscope for use in
photomicrography? I've been asked by my dept. chairman to gather
information. His goal, a departmental camera for everyone to use. As
regards the light photomicrography it would be bright field and
fluorescence. Any information will be appreciated, I'm a complete
newbie
in this digital stuff.

Tom Bargar
CEMRF-Univ. of Neb. Med. Ctr.
tbargar-at-unmc.edu
402-559-7347

From daemon Fri May 24 08:02:34 2002

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Philip and others,

The problem you face comes from the use of immersion lenses in the
modern TEM. The illumination may not be parallel simply because you
spread the beam.

Here are the rules: The true back focal plane can be found by looking
at the Kikuchi lines. When the Kikuchi lines are brought to best
sharpness with the diffraction focus control, you are looking at the
back focal plane. Make this adjustment with the illumination focused at
the specimen plane. If you put the objective aperture in now, it should
give a sharp shadow. If it does not, depending on the model of
microscope that you use, it may be appropriate to adjust the height of
the aperture. If you have the diffraction focus set this way, the beam
will be "parallel" when the spots are sharp.

On the other hand there is no good reason, most of the time, to work in
the back focal plane. Instead, spread the beam to cover the area you
want and make the spots sharp with the diffraction focus. The only
drawback with this is that the camera length is not calibrated.

Here are two publications that may (or may not) help.

'Skew Thoughts on Parallelism'
K. K. Christenson and J. A. Eades
Ultramicroscopy 26 (1988) 113-132

Topics in Electron Diffraction (TEM): A Tutorial
J. A. Eades
Microscopy and Microanalysis 7 Suppl 2 (2001) 764-765

Philip Koeck wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
}
} I'm wondering whether our objective aperture is correctly positioned (or the
} column
} correctly aligned).
} If I focus a diffusely scattering specimen at the eucentric height and then
} switch to diffraction
} mode with a relatively parallel beam (the beam covers the entire screen at a
} magnification of
} about 30K) I can't get a sharp (minimal) central spot and a sharp image of
} the aperture
} at the same time.
} Is there something wrong with our microscope or with my idea of electron
} optics?
}
} sincerely,
}
} Philip Koeck
} Svdertvrns Hvgskola and
} Karolinska Institutet
} Dept. of Bioscience at Novum
} S-14157 Huddinge
} Sweden
} phone: +46-8-6089186
} fax: +46-8-6089290
} http://www.biosci.ki.se/em
}
} The phrase 'We have always done things this way.'
} is as much a reason to change as a reason not to.
} - Dartwill Aquila
} _______________________________________

--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu

From daemon Fri May 24 09:26:23 2002

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Thanks all for the input.
} I figured it must be a typo for photoelectron, but wanted
} to make sure I wasn't missing some *new*
} technique/equipment out there... You never know these
} days. john

From daemon Fri May 24 09:26:24 2002

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,
Once again --thanks to all who responded. Here are the
responses to date:
Tom McGill, Leica
I saw your posting on the listserver just before I left for the day and
thought I would send you a quick email. What you are describing sounds a lot
like a slide marker or object marker.
I am a sales rep for Leica here in Toronto. We have a part # 11505059 and
this is called "Object Marker with thread M25"
It is not a knife per se, but it is a sharp diamond tip that scribes a
circular scratch into the coverslip of a normal slide. You find the area of
interest with the 40x, then swing in the diamond marker, pull down and
rotate the collar, thus marking the coverslip. Take the slide off of the
scope, then you rub India Ink over this scratch.... the ink goes into the
circle and leaves a nice circle so that interesting parts of the slide can
be high-lighted.... similar to the marks that cytotechs put on the slides so
that cytopathologists will know where to look.

Patty Jansma { Neurobiology, Arizona
I haven't heard of exactly what you are looking for but I have seen a
Zeiss "marker" lens similiar to what you are describing. You can flat
embed your cells and find the cell under the compound scope. After that
you rotate in the marker lens and cut the circle around the area of
interest. The difference is that you can remount this area and then
section it.

Sergey Ryazantsev, UCLA
It's out of topic, but it seems to me, people use lasers for such
purpose. You could direct laser using the same lens you are using
for observation and use it as a 'laser blade'. I kind of pessimistic
how you could use proposed tool with x100 lens: distance between lens
and sample is too small. I know tnat our Medical School do have
commercial apparatus for such purpose. I believe it's Olympus (very
expensive).

John Reber, Zeiss
Carl zeiss has a Diamond marker, 462960, $1,385.00. It screws into a standard
microscope nosepiece. The diameter of the circle may be set with a graduated
scale. Engraving takes place by pulling down the spring loaded diamond and
turning the knurled ring.
Regards, John Reber

Gretchen Ziegler, Leica
I believe our company, Leica Microsystems, can do this for you. I will
have a rep get in contact with you.

Chuck Garber, SPI Supplies
For your information we have sold some of our diamond scalpel blades, see
SPI's URL for similar soundingapplications.
However, one has to be really really careful about not damaging the end.
This is something that has happened, and has resulted in at least one
relatively unhappy customer. I don't know what more I could say about this
except I don't see how you could do this with the use of anything else.
Chuck, SPI Supplies

Bob Chiovetti
Most microscope manufacturers can provide a diamond slide marker or diamond
scribe that's used to mark slides...I suppose you could use it to scribe a
plastic section and then float off the scribed area.

The only difficulty would be a lack of control of the circle's size. You
could only scribe one size of circle, whatever the scribe comes equipped to
do. This would make it rather difficult to excise single cells if they are
too close together.

You might want to check out the Eppendorf website. They make a micro-chisel
that's designed for this type of thing. It mounts on the end of a
micromanipulator and also has a micropipette for flushing the chiseled-out
cell and collecting the material. Go to {www.eppendorf.com} and follow the
links: Products -} Cell Technology -} Microdissector.
You can also check with the microscope manufacturers for specialized
instruments that can do the same thing: laser microdissection (Leica), laser
capture (Arcturus), laser catapulting (Zeiss/PALM), etc. Very cool
technology, but also very pricey.
It would probably be quite difficult to pick up the excised single cell in
plastic and transfer to an EM grid, but it might be worth a try. I suppose
you could use either the Leica laser microdissection system of the PALM unit,
since they are non-contact methods, and collect the excised specimen onto a
drop of buffer.
The Arcturus probably wouldn't be suitable, since it depends on heating a
transfer film to make it sticky and then adhering the tissue or specimen to
the film. Good luck! Bob Chiovetti

Edward Principe
Could a dual beam FIB be used to cut your "tiny circle" ? How thick is the
plastic?

Rosemary

--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html

From daemon Fri May 24 09:26:58 2002

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--
Dear listers,

If there is anyone who doesn't mind sharing their fee
structure I would like to know what other electron microscopy
facilities are charging for clinical services. With and without
the professional component.

As always,
Thanks for your input,
Peggy Miller

From daemon Fri May 24 09:56:34 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The May issue of the MSA magazine "Microscopy Today" has a solid article
by Ted Clarke on fitting a retail digital camera to a microscope column.
In it he cites several equally useful articles on the subject. Richard
Harris: if you don't get Microscopy Today, send me a note and I'll send
you a copy of the May issue. You can subscribe at
www.microscopy-today.com.

Ron Anderson, MT Editor

-----Original Message-----
} From: Richard Harris [mailto:rjharris-at-uwo.ca]
Sent: Thursday, May 23, 2002 5:39 PM
To: Microscopy Listserver

Dear Listers;
Does anyone have suggestions on how to fit or couple a retail (like a
Sony
or Cannon) digital video camera to a microscope? Are there any
commercial
products to do this? I've been asked by a Prof who'd like to (on the
cheap)
capture the activities of microscopic marine invertebrates for later
editing
and compiling on his desktop computer. He has a Sony digital camcorder
and
Apple G4 system. I'd really appreciate your feedback.

Thanks;
Richard Harris
Laboratory Supervisor
Imaging and Analysis
Department of Zoology
University of Western Ontario
London ON
CANADA N6A 5B7
(519) 661-2111 ext 86780
(519) 661-2014 Fax

From daemon Fri May 24 15:05:35 2002

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Richard,

We have a universal adapter that will mount the camera on a microscope
trinocular head. This is much better than mounting on the eyepieces
because the camera is more stable. The adapter can screw onto the front
end of most cameras (some will require a step down ring).

It also has a parfocalization adjustment which will allow you to get the
camera and eyepieces in focus at the same time on the microscope.

Please feel free to email me or call if you have any questions or would
like additional information.

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
12901 Duckettown Road
Laurel, MD 20708
voice: (301) 809-6292
fax: (603) 676-2966
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

Richard Harris wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers;
} Does anyone have suggestions on how to fit or couple a retail (like a Sony
} or Cannon) digital video camera to a microscope? Are there any commercial
} products to do this? I've been asked by a Prof who'd like to (on the cheap)
} capture the activities of microscopic marine invertebrates for later editing
} and compiling on his desktop computer. He has a Sony digital camcorder and
} Apple G4 system. I'd really appreciate your feedback.
}
} Thanks;
} Richard Harris
} Laboratory Supervisor
} Imaging and Analysis
} Department of Zoology
} University of Western Ontario
} London ON
} CANADA N6A 5B7
} (519) 661-2111 ext 86780
} (519) 661-2014 Fax

--

From daemon Fri May 24 15:06:43 2002

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I am interested in speaking with someone about support medias (i.e. epoxies, etc.) for moisture and/or oxygen sensitive catalysts
that would allow me to cross-section the catalyst for elemental analysis.

From daemon Fri May 24 18:34:57 2002

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Amy,

to prevent the oxygen exposure, we do the processing and sectioning of
O2-sensitive samples in anaerobic glove bags (Coit, Inc.). We take
advantage of LR White resin properties to polymerize easily at anaerobic
conditions; I prefer the hard grade. I like to complain about the challenge
of anaerobic microtomy (the microtome is placed in a glove bag, and the
controls, as well as the binoculars, are accessed through the gloves /
plastic wall you press your nose against). However, we found out that even
those 15 minutes needed for thin sectioning can make a difference in oxygen
sensitive material chemistry. I guess there is no way to prevent the brief
period in room atmosphere while transferring the grid into the holder. I
transport the grid from the glove box to the TEM room in a sealed container.
Assuming there is no significant oxidation in the TEM vacuum. Make sure all
your processing reagents are anoxic (purged with a gas), as well as the
water in your knife.
Feel free to contact me for more info.
Alice.

Alice Dohnalkova
Environmental Microbiology
Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692 office
(509) 376-3654 TEM lab
fax (509) 376-1321

-----Original Message-----
} From: Weiskittel, Amy L. [SMTP:Amy.Weiskittel-at-Equistarchem.com]
Sent: Friday, May 24, 2002 1:01 PM
To: Microscopy-at-sparc5.microscopy.com

I am interested in speaking with someone about support medias (i.e. epoxies,
etc.) for moisture and/or oxygen sensitive catalysts
that would allow me to cross-section the catalyst for elemental analysis.

From daemon Fri May 24 22:28:38 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What is the best LM staining technique for phenolics in dense hardwoods (core
samples and sledge microtome cut sections)?

From daemon Sat May 25 03:47:58 2002

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Dear Listers,
Greetings of the day.
During a cleanup of our chemical stores I found a package of 1 gm ampules
of Osmium Tetroxide crystals in a large number (presumably they have been
there for over a decade plus). My question is; what is the shelf life
period of these ampules (refrigerated or unrefrigerated). Can they be used
now reliably to make a fixative solution?

A colleague told me that he used one of them in recent times and
complained that as soon as the crystals dissolve in buffer or distilled
water, the solution turns dark immediately (rather than after a prolonged
period)

Regards to all.

M. Yousuf Abdul-Rawoof
Department of Zoology
College of Science
King Saud University
POB 2455, Riyadh 11451
Saudi Arabia

mdyousuf-at-ksu.edu.sa

From daemon Sat May 25 10:48:21 2002

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} I am interested in speaking with someone about support medias (i.e.
} epoxies, etc.) for moisture and/or oxygen sensitive catalysts
} that would allow me to cross-section the catalyst for elemental analysis.

Amy -

Acrylic (LR White, hard) works for some catalysts, epoxy for others; you
can start here:

Csencsits, R., Schooley, C., and Gronsky, R. (1985) An improved method
for thin sectioning of particulate catalysts. J.E.M. Technique 2:643-644.

Ulan, J.G., Schooley, C., & Gronsky, R. (1990) Microtomy of large
particle zeolites for TEM. Mat. Res. Soc. Symp. Proc. 199:153-156

Ulan, J.G., Schooley, C., & Gronsky, R. (1990) Modified embedment
procedure for microtomy of large particle zeolites. J.E.M. Technique
16:254-255

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

From daemon Sun May 26 12:18:31 2002

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Listers,

In the past, a retiring professor donated some 100 1 gram vials that
had been stored for years in his attic, with the attendant harsh
swings in seasonal temperatures in such an environment. The osmium
worked well as I remember. Sure wouldn't hurt to try some of the
vials out and see what happens in water, then buffer. Leave critical
applications for OsO4 that you trust.

Chuck Butterick
Degussa Corporation
Borger, Texas

______________________________ Reply Separator _________________________________

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Dear Listers,
Greetings of the day.
During a cleanup of our chemical stores I found a package of 1 gm ampules
of Osmium Tetroxide crystals in a large number (presumably they have been
there for over a decade plus). My question is; what is the shelf life
period of these ampules (refrigerated or unrefrigerated). Can they be used
now reliably to make a fixative solution?

A colleague told me that he used one of them in recent times and
complained that as soon as the crystals dissolve in buffer or distilled
water, the solution turns dark immediately (rather than after a prolonged
period)

Regards to all.

M. Yousuf Abdul-Rawoof
Department of Zoology
College of Science
King Saud University
POB 2455, Riyadh 11451
Saudi Arabia

mdyousuf-at-ksu.edu.sa

From daemon Sun May 26 17:41:12 2002

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by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id RAA19624
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Philip, Alwyn and others,

I fully agree with Alwyn comments as long as you deal with diffraction. You
just need to recalibrate more often your camera length.

But the "reciprocal issue" that is not solved on modern microscopes with
"twin" lenses or equivalent short focus is the unability to put the
diffraction aperture in hte back focal plane of the objective because of
geometrical constraintgs.

It means that it is not possible to accurately select a diffraction spot,
i.e. to have a true bright field or a clean dark field from a
superstructure reflection.

Moreover at low mag. the shadow of the objective aperture covers the field
of view (it happen up to 100'000x for those tempted by short Cs lenses!).

Only solution: travel to whom has still this good old JEOL 200CX (or buy a
"biologist microscope" with long focal lengths/weak objective lenses if you
agree with 100 kV.

Regards

Philippe Buffat

At 08:56 24.05.2002, Alwyn Eades wrote:
} ------------------------------------------------------------------------
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_______________________________________________________________
Philippe A. Buffat
Ecole Polytechnique Federale de Lausanne (EPFL)
Centre Interdisciplinaire de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/

***NEW*** European Cells and Materials: Free, on-line, peer reviewed journal
http://www.eurocellmat.org.uk/
_______________________________________________________________

From daemon Mon May 27 04:34:12 2002

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I have read on the listserver that discoloured osmium
tetroxide can be regenerated by adding (I think - please
check archives!) hydrogen peroxide.

Dave

On Sun, 26 May 2002 11:50:14 -0500 Chuck Butterick
{cbutte-at-ameripol.com} wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers,
}
} In the past, a retiring professor donated some 100 1 gram vials that
} had been stored for years in his attic, with the attendant harsh
} swings in seasonal temperatures in such an environment. The osmium
} worked well as I remember. Sure wouldn't hurt to try some of the
} vials out and see what happens in water, then buffer. Leave critical
} applications for OsO4 that you trust.
}
} Chuck Butterick
} Degussa Corporation
} Borger, Texas
}
}
} ______________________________ Reply Separator _________________________________
} Subject: Osmium Tetroxide
} Author: mohammed y abdulrawoof / f40z006 75760 {mdyousuf-at-KSU.EDU.SA} at
} INTERNET-MAIL
} Date: 5/25/02 9:16 AM
}
}
} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} Greetings of the day.
} During a cleanup of our chemical stores I found a package of 1 gm ampules
} of Osmium Tetroxide crystals in a large number (presumably they have been
} there for over a decade plus). My question is; what is the shelf life
} period of these ampules (refrigerated or unrefrigerated). Can they be used
} now reliably to make a fixative solution?
}
} A colleague told me that he used one of them in recent times and
} complained that as soon as the crystals dissolve in buffer or distilled
} water, the solution turns dark immediately (rather than after a prolonged
} period)
}
} Regards to all.
}
} M. Yousuf Abdul-Rawoof
} Department of Zoology
} College of Science
} King Saud University
} POB 2455, Riyadh 11451
} Saudi Arabia
}
} mdyousuf-at-ksu.edu.sa
}
}
}
}
}
}
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"

From daemon Mon May 27 07:18:05 2002

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I think it was the buffer or bottle that contained organic matter causing osmium solution to turn black. My osmium crystal had been purchased several decades ago and there is no problem.

Ann Fook

} } } mohammed y abdulrawoof / f40z006 75760 {mdyousuf-at-KSU.EDU.SA} 05/25/02 08:16AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dear Listers,
Greetings of the day.
During a cleanup of our chemical stores I found a package of 1 gm ampules
of Osmium Tetroxide crystals in a large number (presumably they have been
there for over a decade plus). My question is; what is the shelf life
period of these ampules (refrigerated or unrefrigerated). Can they be used
now reliably to make a fixative solution?

A colleague told me that he used one of them in recent times and
complained that as soon as the crystals dissolve in buffer or distilled
water, the solution turns dark immediately (rather than after a prolonged
period)

Regards to all.

M. Yousuf Abdul-Rawoof
Department of Zoology
College of Science
King Saud University
POB 2455, Riyadh 11451
Saudi Arabia

mdyousuf-at-ksu.edu.sa

From daemon Mon May 27 12:03:19 2002

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SuperSTEM � the Second Workshop
Thursday 11th July 2002
part of RMS Microscience 2002, ExCel, London.

The UK SuperSTEM project aims to develop a UK national facility comprising a
suite of ultra high resolution imaging and analytical scanning transmission
electron microscopes (STEMs) at Daresbury Laboratories in Cheshire. The
principle of SuperSTEM is based on the computer controlled aberration
correction of a STEM objective lens using quadrupole and octupole lenses.
This allows the formation of a sub-Angstrom probe for simultaneous ultra
-high resolution high angle annular dark field (HAADF) imaging and
atomic-column electron energy loss spectroscopy (EELS) analysis of thin
specimens.

Following the success of the first SuperSTEM User Workshop at the EMAG 2001
conference at Dundee, the second workshop will be held as part of RMS
Microscience 2002 at the ExCel centre in London and will present the first
results from SuperSTEM I - the first aberration corrected machine which is
currently being tested at Cambridge prior to being moved to a purpose built
facility at Daresbury Laboratories in September 2002. Speakers include Dr
Pete Nellist from NION inc., USA and Dr Andrew Bleloch, technical director
of the SuperSTEM project. We encourage all interested parties to attend.

If you are interested in reserving a place on the workshop then please
email: abeckerl-at-liverpool.ac.uk or write to Mrs A Beckerlegge, SuperSTEM
Secretary, Materials Science and Engineering, University of Liverpool,
Ashton Building, Liverpool L69 3GH, UK.

The registration costs for this second workshop are �25 for the day � please
register with the Royal Microscopical Society
(http://www.microscience2002.org.uk). In addition to the workshop there will
also be other scientific sessions during the day (e.g. energy filtered TEM
imaging, focussed ion beam microscopy, variable pressure SEM and electron
backscattered diffraction) as well as Europe�s largest exhibition of
microscopes and scientific equipment. Further details can be obtained from
the Microscience website or by emailing info-at-rms.org.uk.

--
_____________________________
Dr. Rik Brydson,
Leeds Electron Microscopy and Spectroscopy (LEMAS) Centre
Department of Materials,
University of Leeds,
Leeds LS2 9JT, U.K.

Tel: 44 + (0)113 343 2369
Fax: 44 + (0)113 242 2531
Web: http://www.materials.leeds.ac.uk
Web: http://www.materials.leeds.ac.uk/lemas

New MSc in Nanoscale Science and Technology
Visit http://www.ee.leeds.ac.uk/nanomsc/
______________________________

Bedtime reading..........
Series: Microscopy Handbooks - Electron Energy Loss Spectroscopy by Rik
Brydson, BIOS
Published: September 2001 �29.99 ISBN: 1859961347
Paperback; 152 pages 39 line drawings and 10 b/w half tones

**************************
Join email discussion list for all aspects of
Electron Microscopy and Analytical Spectroscopy (LEMAS)

visit website
http://www.jiscmail.ac.uk/lists/lemas.html
and follow instructions
**************************

=====================================
EELS and X ray database : http://www.cemes.fr/~eelsdb/
=====================================

--

From daemon Mon May 27 12:29:41 2002

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Hi Greg,

I'm afraid it's impossible to answer your question. The number of of
polyphenol is so high and can be found in so many different forms in very
different cells compartment (walls, vacuoles) associated with proteins,
sugars ... that there no single, best stain which will give you the right
answer...

In my PhD, I tried to quantify the proteins, the tannins, amyloplasts in the
bark and the xylem (last increments) of 100 adult european beeches. I used
image analysis and ... fluorescence microscopy (without staining, only with
autofluorescence).

As preliminary tests I tried some light microscopy staining which could help
you:

First you can try some old tests such as the vanillin test, the nitrous test
(Reeve, 1951; Jensen, 1962)...

If you fix your samples in Glutaraldehyde / paraformaldehyde, you will see
condensed tannins (yellow) in the cells vacuoles. If you try to stain these
fixed sections with toluidine blue (pH 4.4), these vacuoles will stain in
blue-green and the proteins in blue (the difference is not easy to
see)...Look at FEDER et O'BRIEN 1968 or O'BRIEN et al. 1964.

Some polyphenols will be black if you stain them with DMCA
(di-methyl-amino-cinnamaldehyde, also used in fluorescence microscopy).

If you simply want to localise the heartwood on a core you can perhaps
simply try to stain with lugol (there are only few amyloplasts or starch in
the heartwood).

Some people on International Tree Ring Forum (ITRDBFOR-at-listserv.arizona.edu)
will certainly give you better advices than me. Look on the net to see how
to register...

Some of the references below could perhaps help you (I do no more work on
plants and I have no more access on these articles: I don't remember the
content of these articles).

Hope it helps !!!

Chris Wuethrich
Beth Israel Deaconess Medical Center
330 Brookline AVE
Boston, MA 02215
cwuethri-at-caregroup.harvard.edu

FEUCHT W, SCHMID PPS, CHRIST E. 1986. Distribution of Flavonols in
Meristematic and Mature Tissues of Prunus avium shoots. J. Plant Physiol.
125:1-8.

FEUCHT W, TREUTTER D, CHRIST E. 1997. Role of flavanols in yellowing beech
trees of the Black Forest. Tree Physiol. 17:335-340.

FRANCESCHI VR, KREKLING T, BERRYMAN AA, CHRISTIANSEN E. 1998. Specialized
phloem parenchyma cells in norway spruce (Pinaceae) bark are an important
site of defense reactions. Am. J. Bot. 85(5):601-615.

GETACHEW G, MAKKAR HP, BECKER K. 1998. Localisation and quantification of
tannins in multipurpose tree leaves using a histochemical approach coupled
with image analysis (http://ecsoc2.hcc.ru.poster/DP_TOP6/dp278/dp278.htm).

MANSFIELD JL, CURTIS PS, ZAK DR, PREGITZER KS. 1999. Genotypic variation for
condensed tannin production in trembling aspen (Populus tremuloides,
Salicaceae) under elevated CO2 and in high- and low-fertility soil. Am. J.
Bot. 86(8):1154-1159.

MUZIKA RM, PREGITZER KS. 1992. Effect of nitrogen fertilization on leaf
phenolic production of grand fir seedlings. Trees 6:241-244.
NAGARAJAN GR, SESHADRI TR. 1964. Flavonoid components of the heartwood of
Prunus domestica Linn. Phytochem. 3:477-484.

EL MODAFAR C, CLERIVET A, MACHEIX JJ. 1996. Flavan accumulation in stems of
Platanus x acerifolia seedlings inoculated with Ceratocystis fimbriata f.sp.
platani, the canker stain disease agent. Can. J. Bot. 74:1982-1987.

GROSSONI P, BUSSOTTI F, TANI C, GRAVANO E, SANTARELLI S, BOTTACI A. 1998.
Morpho-anatomical alterations in leaves of Fagus sylvatica L. and Quercus
ilex L. in different environmental stress conditions. Chemosphere
36(4-5):919-924.

From daemon Mon May 27 20:32:07 2002

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Hi,

About a year ago I asked if anyone had done any work on the in situ
generation of osmium tetroxide. I got a few replies but nobody had a
procedure.

I stained a lot of polymers with various chemicals in the past 12 years. I
did not like to store RuO4 or OsO4 ampoules in my lab. I had an RuO4
ampoule break open in my refrigerator once. I lab-packed the remaining
ones and switched to in situ generation of RuO4 as a safer alternative.

So, I recently investigated the reactions of osmium chloride and dioxide
with various oxidizers for use on physical science samples. In my
experiments, I used about 1-3 milligrams per reaction test in a Petri dish.
Five reaction routes worked.

Discussion:
An indicator to gauge the progress of the reaction was made by dissolving
Budene� 1207 (Goodyear Tire and Rubber Company) rubber in cyclohexane
solvent. This almost colorless gum stock rubber consisted of a butadiene
rubber containing plenty of double bonds to react. This very thick
solution (almost a gel) was smeared onto a glass slide and allowed to
evaporate. This clear fairly thin film indicator system turned
progressively darker as the staining reaction proceeded and more vapors of
OsO4 were generated. It ultimately turned the indicator black on overnight
exposures.

It was observed that osmium dioxide reacted slower than osmium chloride and
both were much slower than RuCL3.xH2O. I wanted the osmium dioxide to work
and it did. However, it was very slow and required overnight exposures at
the 1-2 mg level. Don't use hydrogen peroxide with osmium dioxide powder.
It created a vigorous decompositional foaming of the hydrogen peroxide with
little generated OsO4, if any.

Osmium chloride reacted with sodium periodate (fastest), sodium
hypochlorite and hydrogen peroxide (slowest) to stain the butadiene rubber
indicator. The use of osmium chloride and hypochlorite seemed the best
choice because both chemicals were the easiest to obtain. The periodate
was a powder one had to mix and the peroxide could cause serious burns of
the skin. On the other hand, if one spilled hypochlorite on your arm, not
much would happen. The osmium chloride was not as easy to handle as RuCl3.
Reading the OsCl3 MSDS sheet could give one heart failure. There was no
free lunch here. The use of gloves, avoidance of skin contact and the
prevention of airborne dusts were critical with osmium compounds also.

In order to help gauge the progress of the reaction, it helped to have a
couple of paper towels and lastly a poly-jean cloth under the covered
reaction dishes. This provided a nice white background to see a light
stain on the indicator slide.
If the dishes broke, then only a milliliter or two of hypochlorite with a
few milligrams of osmium tetroxide would soak into the cloth and paper
towels in your hood. Cleanup would not require a HAZMAT team.

This relatively safer reaction avoided storing large amounts of Os in
opened ampoules or jars containing unused osmium tetroxide. It also
avoided managing a larger amount of waste osmium.

Disclaimer: My employer and I are not responsible for your use or safety
in handling these highly toxic and hazardous materials. Only you can
manage your risks. You must use gloves, use a fume hood, read the MSDS
sheets, avoid dusts, avoid skin, lung and eye exposures, etc. With a TWA
exposure level 2000 times lower than phosgene gas, you should get the
message that these are not casual chemicals to use without training. Talk
to someone that has previously used these materials and is a safe worker.

A more detailed procedure for generating and handling RuO4 and OsO4 was
written but apparently can't be published without tons of clearances.

Paul Beauregard
Senior Research Associate Microscopist

From daemon Tue May 28 03:12:13 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

LM Need help on staining glucans in cell walls. Do you know any "specific"
stain for glucans?.

Thanks,

Jes�s Lamas

From daemon Tue May 28 06:51:09 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers:

I need to learn more about ion milling for TEM sample preparation. What is
considered to be the optimum milling condition with respect to voltage,
specimen current and temperature for materials which minimises beam damage
and maximises milling rate? Is it just by trial and error or are there
specific rules to consider for different classes of materials to determine
the optimum milling conditions?

----
R Divakar

From daemon Tue May 28 07:49:37 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
Like Ann, I think that the problem is your buffer or your bottle. Osmium reacts strongly with lipids and turns black right away. So to avoid that, we used extraclean bottles, always wear gloves before touching anything related to the osmium solution (you have lipids on your hands, plus osmium is highly toxic) and rinse really well with acetone the outside of the vials that contain osmium. Hope this helps.
Emmanuelle

Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620

From daemon Tue May 28 09:35:26 2002

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Hi Jesus,

Try 0.01-0.2% Ruthenium red in an acid solution (for example acetic acid or
S�rensen buffer) and rince with the same acidic solution or buffer. In
acidic solution ruthenium red will stain "specifically pectins. If I
remember well non methylated pectins. I guess there is another stain which
will stain more specifically the methylated pectins (try perhaps to look at
articles by Anne-Marie Catesson et al.).

As Ruthenium red is expensive, you can try to do the same with Astra Blue.
As I have seen (on 5�m beech sections embedded in JB4 resin), it will do the
same as Ruthenium red, but in bright blue...

Important notice: The fixation of the staining is reversible. In a basic
solution, the stain of the walls will migrate in the vacuoles (more acid)...
Be careful when you mont your slides that the medium is not too basic...

Hope it helps

Chris Wuethrich
Beth Israel Deaconess medical Center
330 brookline AVE
Boston, MA 02215
cwuethri-at-caregroup.harvard.edu

From daemon Tue May 28 10:11:57 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul,

Nice work. I, too, have worked extensively with in-situ production of RuO4
but never with OsO4. This will be very useful.

I applaud the sharing of your work on the list server.

Best regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com

Beauregard
{beaurega-at-westo To: microscopy-at-sparc5.microscopy.com
l.com} cc: peoshel-at-facstaff.wisc.edu
Subject: In situ generation of Osmium
Tetroxide for the Physical Sciences.
05/27/02 08:24
PM

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hi,

About a year ago I asked if anyone had done any work on the in situ
generation of osmium tetroxide. I got a few replies but nobody had a
procedure.

I stained a lot of polymers with various chemicals in the past 12 years. I
did not like to store RuO4 or OsO4 ampoules in my lab. I had an RuO4
ampoule break open in my refrigerator once. I lab-packed the remaining
ones and switched to in situ generation of RuO4 as a safer alternative.

So, I recently investigated the reactions of osmium chloride and dioxide
with various oxidizers for use on physical science samples. In my
experiments, I used about 1-3 milligrams per reaction test in a Petri dish.
Five reaction routes worked.

Discussion:
An indicator to gauge the progress of the reaction was made by dissolving
Budene� 1207 (Goodyear Tire and Rubber Company) rubber in cyclohexane
solvent. This almost colorless gum stock rubber consisted of a butadiene
rubber containing plenty of double bonds to react. This very thick
solution (almost a gel) was smeared onto a glass slide and allowed to
evaporate. This clear fairly thin film indicator system turned
progressively darker as the staining reaction proceeded and more vapors of
OsO4 were generated. It ultimately turned the indicator black on overnight
exposures.

It was observed that osmium dioxide reacted slower than osmium chloride and
both were much slower than RuCL3.xH2O. I wanted the osmium dioxide to work
and it did. However, it was very slow and required overnight exposures at
the 1-2 mg level. Don't use hydrogen peroxide with osmium dioxide powder.
It created a vigorous decompositional foaming of the hydrogen peroxide with
little generated OsO4, if any.

Osmium chloride reacted with sodium periodate (fastest), sodium
hypochlorite and hydrogen peroxide (slowest) to stain the butadiene rubber
indicator. The use of osmium chloride and hypochlorite seemed the best
choice because both chemicals were the easiest to obtain. The periodate
was a powder one had to mix and the peroxide could cause serious burns of
the skin. On the other hand, if one spilled hypochlorite on your arm, not
much would happen. The osmium chloride was not as easy to handle as RuCl3.
Reading the OsCl3 MSDS sheet could give one heart failure. There was no
free lunch here. The use of gloves, avoidance of skin contact and the
prevention of airborne dusts were critical with osmium compounds also.

In order to help gauge the progress of the reaction, it helped to have a
couple of paper towels and lastly a poly-jean cloth under the covered
reaction dishes. This provided a nice white background to see a light
stain on the indicator slide.
If the dishes broke, then only a milliliter or two of hypochlorite with a
few milligrams of osmium tetroxide would soak into the cloth and paper
towels in your hood. Cleanup would not require a HAZMAT team.

This relatively safer reaction avoided storing large amounts of Os in
opened ampoules or jars containing unused osmium tetroxide. It also
avoided managing a larger amount of waste osmium.

Disclaimer: My employer and I are not responsible for your use or safety
in handling these highly toxic and hazardous materials. Only you can
manage your risks. You must use gloves, use a fume hood, read the MSDS
sheets, avoid dusts, avoid skin, lung and eye exposures, etc. With a TWA
exposure level 2000 times lower than phosgene gas, you should get the
message that these are not casual chemicals to use without training. Talk
to someone that has previously used these materials and is a safe worker.

A more detailed procedure for generating and handling RuO4 and OsO4 was
written but apparently can't be published without tons of clearances.

Paul Beauregard
Senior Research Associate Microscopist

From daemon Tue May 28 10:16:05 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
Information on the Microscopical Society of Ireland Symposium for
2002 and abstract submission forms etc. is now up at
www.nuigalway.ie/msi

Sincerely,
Alexander Black

From daemon Tue May 28 10:25:44 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For ceramics and minerals the damage rate varies considerably.
I use 6 keV for most minerals except oxides.
Oxides require 0.5 keV for 24 hours and LN2 cooling stage to remove
surface damage.
Silicates on the other hand can thin at 6 keV and suffer minimum damage.

Temperatures can get fairly high so if you are interested in
transformation microstructures be aware.
A LN2 stage is very useful.

As a rule of thumb you get the best results it you start with a thin
material.
10 micrometers thick is a good start, if possible.

Your results will vary, speaking from would you believe, 30 years of
experience.

Gordon Nord
Environmental Sciences Laboratory
Brooklyn College

On Tuesday, May 28, 2002, at 07:43 AM, Divakar R wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers:
}
} I need to learn more about ion milling for TEM sample preparation. What
} is
} considered to be the optimum milling condition with respect to voltage,
} specimen current and temperature for materials which minimises beam
} damage
} and maximises milling rate? Is it just by trial and error or are there
} specific rules to consider for different classes of materials to
} determine
} the optimum milling conditions?
}
}
} ----
} R Divakar
}
}
}
}

From daemon Tue May 28 10:43:45 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jes�s, Do you want to stain beta 1,3 or 1,4 glucans. And then do you prefer
antibodies or fluoroscent stains. There are stains such as Calcofluor,
Aniline blue and Sirofluor stains either one of these or both glucans.
Monoclonal antibodies are also available from
http://www.biosupplies.com.au/product.html, an Australian company. Do you
want to use with plants, yeast or in human cells to study activation of
immune response involving macrophages. If you could be more specific with
detail may help answering better.

Shiv

Dr. Mayandi Sivaguru, Ph.D.,
Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2, Tucker Hall
University of Missouri
Columbia, MO 65211-7400
USA

Voice: 1-573-882-4895
Fax: 1-573-882-0123
Home: 1-573-445-6037

www.biotech.missouri.edu/mcc/

Following are the sample products from Biosupplies might be relevant to you.
Enzymes:
(1-3;1-4)-beta-D-Glucan Hydrolase from Bacillus subtilis (EC 3.2.1.73).
Specifically hydrolyses beta-D-glucans containing both (1-3)- and
(1-4)-beta-D-glucosidic linkages in linear sequences. Does not hydrolyse
(1-4)-beta-D-glucans or (1-3)-beta-D-glucans [Anderson & Stone (1975) FEBS
Letters 52: 202-207].

Substrates:
Pachyman [(1-3)-beta-D-Glucan] ex Poria cocus
Useful as a positive control in fluoroescence microscopy studies on callose
using the aniline blue fluorochrome or (1-3)-beta-D-glucan specific
monoclonal antibody.
Carboxymethyl-pachyman [(1-3)-beta-D-Glucan] (D.S. 0.31)
This water-soluble O-carboxymethyl ether of pachyman is a useful substrate
for assay of (1-3)-beta-D-glucan endo-hydrolases either reductometrically
or viscometrically.
(1-3;1-4)-beta-Glucan from Barley
A water-soluble polysaccharide from walls of barley endosperm cells. Useful
as a substrate for (1-3;1-4)-beta-D-glucan hydrolase and as a control in
immunohistochemical studies with the (1-3;1-4)-beta-D-glucan monoclonal
antibody [Meikle et al (1994) The Plant Journal 5: 1-9].
Arabino-(1-4)-beta-D-Xylan from wheat flour
A water-soluble polysaccharide from walls of wheat endosperm cells. Useful
as a substrate for (1-4)-beta-D-xylan hydrolase.

Histochemical Reagents:
Aniline Blue Fluorochrome for callose
An analytical probe for (1-3)-beta-D-glucans (callose). Highly specific for
(1-3)-beta-glucans [Evans et al. (1984) Carbohydrate Polymers 4: 215-230;
Stone et al. (1984) Protoplasma 122: 191-195]. May be used for the
quantitative determination of callose [Kauss (1989) Plant Physiol. 81:
171-176].
Yariv Reagents for arabinogalactan-proteins
Specific probes for the detection of arabinogalactan-proteins in tissue
sections [Anderson et al. (1977) Aust J. Plant Physiol. 4: 143-158]. May be
used to detect and quantify arabinogalactan-proteins in tissue extracts
[Van Holst & Clarke (1985) Anal Biochem. 148: 446-450] and to detect
arabinogalactan-proteins in crossed-electrophoretic separations [Van Holst
& Clarke (1986) Plant Physiol. 80: 786-798]

Immunohistochemical Reagents:
Monoclonal Antibodies (Murine)
to (1-3)-beta-D-Glucan
No cross-reactivity with (1-4)-beta-D-glucans or (1-3;1-4)-beta-D-glucans
[Meikle et al. (1991) Planta 188: 1-8].
to (1-3;1-4)-beta-D-Glucan
No cross-reactivity with (1-3)-beta-D-glucans [Meikle et al. (1994) The
Plant Journal 5: 1-9].
Each of these antibodies can be used with second stage, gold- or
fluorochrome-labelled rabbit, anti-mouse antibody for immunohistochemical
studies.

From daemon Tue May 28 11:06:00 2002

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,

On August 2 and 3, 2002, there will be a workshop on Characterization of
Non-Conductive or Charging Materials with Microbeam Analysis. This workshop
will be held at McGill University which is located in Montreal, Quebec,
Canada. It is still possible to register to this workshop, the deadline
have been postpone up to the end of July. For information about lodging and
update of the scientific program, please go to

http://www.minmet.mcgill.ca/charging

Also note that on Saturday, august 3 after 3h00 pm, it will be possible to
attendees to take a special bus to go to Quebec city. The cost will be 25$
canadian.

I will be please to meet you at McGill next august.

Raynald GAUVIN

From daemon Tue May 28 11:46:11 2002

Contents Retrieved from Microscopy Listserver Archives
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Hi, Friends,

I have an old link EDX detector made in England. Its series no is
23088-1380-01. ATT NO: 32CC6080D, 0120024. Model: 6547. Det. Area: 30
mmxmm. Winder: ATW2. Resolution: 136 at 5.9 KeV.

Currently, the X-ray count rate is quite low (less than 200 counts/sec)
even when beam was strong and sample was thick. This problem occurred
when our Link pulse processor (QX 2000) was replaced by a new pulse
processor produced by a new company. I thought the new pulse processor
did not get along with the Link detector, which caused the problem.
After the investigation of several months, an engineer from the company
told me that my detector suffered from hard X-rays or backscattered
electrons which hit the detector. The hard X-rays could frequently reset
the EDX system and cause the low count rate.
Therefore, the low rate may be related to both the detector and the
processor. First I would like to know your opinion about possible
reasons about the hard X-ray and back-scattering electrons. Second, I
need a Link pulse processor (XQ2000) (mine was dead) to see the
performance of the detector. Please let me know if you have one and no
longer use it. I want to identify the reasons one by one. Any other
suggestions are welcome.

I am looking forward to your response.

Jian-Guo Zheng
EPIC
Materials Science & Engineering
Northwestern University
2225N Campus Drive, 1156 Cook Hall
Evanston, IL 60208-3108, USA
Phone: (847) 491-7807, Fax: (847) 491-7820
E-mail: j-zheng3-at-northwestern.edu

From daemon Tue May 28 12:29:53 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'll second that
I also suggest this gets written up in Microscopy Today with Paul's
name on it
Chris
Paul,

Nice work. I, too, have worked extensively with in-situ production of
RuO4
but never with OsO4. This will be very useful.

I applaud the sharing of your work on the list server.

Best regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com

Beauregard
{beaurega-at-westo To:
microscopy-at-sparc5.microscopy.com
l.com} cc:
peoshel-at-facstaff.wisc.edu
Subject: In situ
generation of Osmium
Tetroxide for the Physical
Sciences.
05/27/02 08:24
PM

----------------------------------------------------------------------
--
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

Hi,

About a year ago I asked if anyone had done any work on the in situ
generation of osmium tetroxide. I got a few replies but nobody had a
procedure.

I stained a lot of polymers with various chemicals in the past 12
years. I
did not like to store RuO4 or OsO4 ampoules in my lab. I had an RuO4
ampoule break open in my refrigerator once. I lab-packed the
remaining
ones and switched to in situ generation of RuO4 as a safer
alternative.

So, I recently investigated the reactions of osmium chloride and
dioxide
with various oxidizers for use on physical science samples. In my
experiments, I used about 1-3 milligrams per reaction test in a Petri
dish.
Five reaction routes worked.

Discussion:
An indicator to gauge the progress of the reaction was made by
dissolving
Budene� 1207 (Goodyear Tire and Rubber Company) rubber in cyclohexane
solvent. This almost colorless gum stock rubber consisted of a
butadiene
rubber containing plenty of double bonds to react. This very thick
solution (almost a gel) was smeared onto a glass slide and allowed to
evaporate. This clear fairly thin film indicator system turned
progressively darker as the staining reaction proceeded and more
vapors of
OsO4 were generated. It ultimately turned the indicator black on
overnight
exposures.

It was observed that osmium dioxide reacted slower than osmium
chloride and
both were much slower than RuCL3.xH2O. I wanted the osmium dioxide to
work
and it did. However, it was very slow and required overnight
exposures at
the 1-2 mg level. Don't use hydrogen peroxide with osmium dioxide
powder.
It created a vigorous decompositional foaming of the hydrogen peroxide
with
little generated OsO4, if any.

Osmium chloride reacted with sodium periodate (fastest), sodium
hypochlorite and hydrogen peroxide (slowest) to stain the butadiene
rubber
indicator. The use of osmium chloride and hypochlorite seemed the
best
choice because both chemicals were the easiest to obtain. The
periodate
was a powder one had to mix and the peroxide could cause serious burns
of
the skin. On the other hand, if one spilled hypochlorite on your arm,
not
much would happen. The osmium chloride was not as easy to handle as
RuCl3.
Reading the OsCl3 MSDS sheet could give one heart failure. There was
no
free lunch here. The use of gloves, avoidance of skin contact and the
prevention of airborne dusts were critical with osmium compounds also.

In order to help gauge the progress of the reaction, it helped to have
a
couple of paper towels and lastly a poly-jean cloth under the covered
reaction dishes. This provided a nice white background to see a light
stain on the indicator slide.
If the dishes broke, then only a milliliter or two of hypochlorite
with a
few milligrams of osmium tetroxide would soak into the cloth and paper
towels in your hood. Cleanup would not require a HAZMAT team.

This relatively safer reaction avoided storing large amounts of Os in
opened ampoules or jars containing unused osmium tetroxide. It also
avoided managing a larger amount of waste osmium.

Disclaimer: My employer and I are not responsible for your use or
safety
in handling these highly toxic and hazardous materials. Only you can
manage your risks. You must use gloves, use a fume hood, read the
MSDS
sheets, avoid dusts, avoid skin, lung and eye exposures, etc. With a
TWA
exposure level 2000 times lower than phosgene gas, you should get the
message that these are not casual chemicals to use without training.
Talk
to someone that has previously used these materials and is a safe
worker.

A more detailed procedure for generating and handling RuO4 and OsO4
was
written but apparently can't be published without tons of clearances.

Paul Beauregard
Senior Research Associate Microscopist

From daemon Tue May 28 20:45:28 2002

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From daemon Tue May 28 22:41:21 2002

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The samples I am trying to prepare for HRTEM are nanocrystalline palladium.

Best Wishes,
Divakar

-----Original Message-----
} From: Peter Tomic [SMTP:PTomic-at-anadigics.com]
Sent: Wednesday, May 29, 2002 9:08 AM
To: Divakar R

Dear Listers:

I need to learn more about ion milling for TEM sample preparation. What is
considered to be the optimum milling condition with respect to voltage,
specimen current and temperature for materials which minimises beam damage
and maximises milling rate? Is it just by trial and error or are there
specific rules to consider for different classes of materials to determine
the optimum milling conditions?

----
R Divakar

From daemon Wed May 29 03:36:42 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jian-Guo,

A few thoughts for you.

An important question is - why has this problem started
since you changed the processor, surely the problem woud
have existed before if it is a microscope or a detector
shielding problem?

You do not say what instument your detector is on. From the
crystal size I would guess that it is on a TEM is this
correct? If not then you can discount hard X-rays and
backscattered electrons. Can you operate at a much lower
voltage and improve the problem, x-rays can only be as
energetic as the accelerating voltage?

I assume that the objective apertures and all other
possible sources of BSEs and x-rays in the secimen region
are out. Do you have a 'hard x-ray aperture' above the
specimen or in the condensor strip that should be used? Are
you in a mag range and not in low mag when the objective
lens is weak and may allow more electrons to hit the
detector?

If you get high energy x-rays or electrons into the
detector you will get low counts but very high dead time as
the detector is spending most of it's time recognising and
rejecting unsuitable events (not in the 0-20kV range).

However, I would expect the operation without the
microscope beam on to be fine, that is the zero strobe or
noise peak should be a few hundred cps, good resolution (50
to 60eV in your case) and no other signals.

If this changes when the beam is on but the specimen is not
in it may be high energy x-rays. Retracting the detector
should change (but may not eliminate) this.

If you get a high dead time with low counts when the
specimen is in can you reduce the beam current and reduce
the counts? Does changing the processing time change the
deadtime significantly? If counts increase as the beam
current drops then you have too many counts and should keep
reducing the beam current to get a good operating condition.

It is possible to recognise electrons or high energy x-rays
if you can look at the processor ramp with an oscilloscope.
In an EDX detector the crystal operates as a reverse bias
diode, when is has no x-ray input there will be a voltage
ramp that is reset at a certain voltage. This will be seen
as a sawtooth wave form with a period of milliseconds
(maybe several 100s of ms). When an x-ray is detected the
ramp has a jump equivalent to the energy of the x-ray. If
an electron enters the ramp may see the negative charge of
the electron at the leading edge of the jump. If either an
electron or a high energy x-ray enters the jump height will
be very large. This causes the ramp to reset very often and
gives a high deadtime. Your engineer, who is telling you
that it is a high energy or x-ray problem, should be able
to demonstrate this to you and determine which it is.

If it really is caused by electrons or high energy x-rays
this is best discussed with you microscope manufacturer. I
still wonder why it was not a problem before, maybe a look
in the column is needed, collimator damaged, lost specimen
blocking x-ray beam etc. and probe is very strong as you
don't have a 'real' x-ray sgnal from your specimen.

Good luck,
Ron

On Tue, 28 May 2002 11:36:39 -0500 Jian-Guo Zheng
{j-zheng3-at-northwestern.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, Friends,
}
} I have an old link EDX detector made in England. Its series no is
} 23088-1380-01. ATT NO: 32CC6080D, 0120024. Model: 6547. Det. Area: 30
} mmxmm. Winder: ATW2. Resolution: 136 at 5.9 KeV.
}
} Currently, the X-ray count rate is quite low (less than 200 counts/sec)
} even when beam was strong and sample was thick. This problem occurred
} when our Link pulse processor (QX 2000) was replaced by a new pulse
} processor produced by a new company. I thought the new pulse processor
} did not get along with the Link detector, which caused the problem.
} After the investigation of several months, an engineer from the company
} told me that my detector suffered from hard X-rays or backscattered
} electrons which hit the detector. The hard X-rays could frequently reset
} the EDX system and cause the low count rate.
} Therefore, the low rate may be related to both the detector and the
} processor. First I would like to know your opinion about possible
} reasons about the hard X-ray and back-scattering electrons. Second, I
} need a Link pulse processor (XQ2000) (mine was dead) to see the
} performance of the detector. Please let me know if you have one and no
} longer use it. I want to identify the reasons one by one. Any other
} suggestions are welcome.
}
} I am looking forward to your response.
}
} Jian-Guo Zheng
} EPIC
} Materials Science & Engineering
} Northwestern University
} 2225N Campus Drive, 1156 Cook Hall
} Evanston, IL 60208-3108, USA
} Phone: (847) 491-7807, Fax: (847) 491-7820
} E-mail: j-zheng3-at-northwestern.edu
}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk

From daemon Wed May 29 15:44:07 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

We have a Hummer VII sputter coater, and I know the oil has not been changed in many years. I wanted to change the oil, but have no instructions on how to do this - and the person who was responsible for this machine is now gone. The drain and fill points are obvious, but are there any things that I need to be aware of before doing this? How do I go about ballasting the oil? Please bear with the questions - as in the past - I was only a user of the equipment - not someone who has experience in maintaining it.

Thanks for any information!

Susan

Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, N.S. B4N 1J5
Canada

Phone (902) 679-5535
Fax (902) 679-2311

E-Mail: carbyns-at-em.agr.ca

From daemon Wed May 29 16:01:44 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Regarding my Toluidine Blue stain solution, which should be staining animal
tissue varying shades of blue-purple (which I want), but is now staining
bright blue instead (which I don't want):

1% Toluidine Blue O
1% Sodium Tetraborate Dodecahydrate
30% Ethanol
in DDI

(1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)

I've received several suggestions to change the pH--some to acidic, some to
basic. Which direction should it be changed and what chemical do I use to
change it?

Thank you again,

Jaclynn M. Lett, Research Technician
Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu
314-977-0257

From daemon Wed May 29 16:39:06 2002

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Depends on the type of sections. You only need the tetraborate (which makes
the stain very alkaline) if you are trying to stain through epoxy (Epon,
Araldite, Spurr) sections. If you want metachromasia (varing shades of
blue/purple/red), you must have an acidic solution. If you are staining wax or
frozen sections, eliminate the tetraborate, eliminate the alcohol, and cut the
stain concentration to 0.1% or less in distilled water (I use 0.05%), rinse in
water and dehydrate in acetone, not alcohol, to preserve the metachromasia. You
can use various buffers to get the pH lower than 4 for even more selective
staining.
If you are staining epoxy sections, keep the tetraborate but eliminate the
alcohol. Alcohol destroys metachromasia, at least in TB stained tissue.

Jaclynn Lett wrote:

} Regarding my Toluidine Blue stain solution, which should be staining animal
} tissue varying shades of blue-purple (which I want), but is now staining
} bright blue instead (which I don't want):
}
} 1% Toluidine Blue O
} 1% Sodium Tetraborate Dodecahydrate
} 30% Ethanol
} in DDI
}
} (1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)
}
} I've received several suggestions to change the pH--some to acidic, some to
} basic. Which direction should it be changed and what chemical do I use to
} change it?
}
} Thank you again,
}
} Jaclynn M. Lett, Research Technician
} Electron Microscopy Core Facility
} Fay and Carl Simons Center for Biology of Hearing and Deafness
} Central Institute for the Deaf
} 4560 Clayton Ave.
} St. Louis, MO 63110
}
} jlett-at-cid.wustl.edu
} 314-977-0257

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************

From daemon Wed May 29 17:35:13 2002

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There are a number of things that are important with respect to ion milling. Since you are doing HREM, you want to minimize surface damage and since you want to work with nanocrystalline material, you want to minimize beam heating to prevent grain growth or phase changes. As you increase the milling angle, you increase the rate of etching up to a maximum at about 20 degrees, however, several significant things will happen, 1) the sample heating goes up, 2) the surface damage by ion implantation goes up, and 3) topographic features are enhanced and can grow. Going to a lower energy at these high angles can improve things, but not by much. The best thing to do is to go to low angles and low voltage. Both of these will minimize the effects above and the low angle will "smooth" features out and actually polish the sample, but at the expense of time. That is why the low angle ion mills need and have higher current ion guns than the older ion mills.

To speed things up a little, you can go to a higher angles to mill for awhile, drop the angle to the lowest that you can go (and still hit the desired area), and then finish at a lower energy. On the newer mills, I would not go above 10 degrees to start. I typically will start at 6-8 degrees and drop to about 4 degrees. This is the lowest that my dimple + sample holder will allow me. Since I use a single sided dimple, I can have the gun on the flat side of the sample at a little lower angle. Fortunately, my samples are glass and I can see the samples fluoresce when the beam hits them. My samples are also thinned as thin as they permit and still be able to handle them so that the dimple depth is as shallow as possible. This also decreases the lowest possible angle. I really don't like to go above 5 kV, but when I am in a bit more of a hurry, I will start at 6 kV. I finish at about 2.5 kV at 4 degrees. One thing that you should remember, that most of the guns on commercially
available ion mills lose their focus at lower voltages, i.e. the beam broadens and the current density decreases. The one exception is the Gentle Mill from South Bay Technology that uses Arpa Barna's low energy guns. The main thing to realize for your HREM application is that the damage layer on top and bottom of your sample will be minimized the lower the voltage and the lower that angle. There is a considerable difference in the available mills on the market with respect to the milling rates at different voltages and lowest possible angles.

I would recommend that you take a piece of soda lime glass (ordinary window glass) and prepare a sample to the same thickness and dimple it to the same dimensions that you will use on your sample. Then lower the ion mill angle to the lowest that you can and still see the center of the dimple lit up. With a dimple in the sample, you will actually see a shadow come across the center of the dimple when the shoulder of the dimple is shadowing the beam. This will give you an indication for the lowest angle that you can get to. A glass sample will also serve as an alignment sample for the ion guns. Nothing slows down ion milling better than guns that are out of alignment. This sample is best if you just use about a 100 um thick sample that is parallel sided.

I also suggest that you get a hold of the third installment of the MRS books on TEM sample preparation and look at Arpa Barna's paper. In the words of Ron Anderson, "He taught the world how to ion mill."

I hope this helps.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

-----Original Message-----
} From: Divakar R [mailto:divakar-at-igcar.ernet.in]
Sent: Tuesday, May 28, 2002 7:44 AM
To: microscopy-at-sparc5.microscopy.com

Dear Listers:

I need to learn more about ion milling for TEM sample preparation. What is
considered to be the optimum milling condition with respect to voltage,
specimen current and temperature for materials which minimises beam damage
and maximises milling rate? Is it just by trial and error or are there
specific rules to consider for different classes of materials to determine
the optimum milling conditions?

----
R Divakar

From daemon Wed May 29 18:57:59 2002

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G'day All,
For epoxy sections you require high pH Tol. Blue stain and heat ~60c.
After staining, rinse the sections and dry the slide before
coversliping.
Do not use alcohol or excessive heat to dry the slide, both will destroy
the metachromacy. You require some water in the section for metachromacy
to remain viable.
Best to mount the coverslip in a water based mounting media, this
prevents the bleaching of the tol.blue stain that so often happens with
xylene based styrene mountants.
Regards
JVN

Geoff McAuliffe wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Depends on the type of sections. You only need the tetraborate (which makes
} the stain very alkaline) if you are trying to stain through epoxy (Epon,
} Araldite, Spurr) sections. If you want metachromasia (varing shades of
} blue/purple/red), you must have an acidic solution. If you are staining wax or
} frozen sections, eliminate the tetraborate, eliminate the alcohol, and cut the
} stain concentration to 0.1% or less in distilled water (I use 0.05%), rinse in
} water and dehydrate in acetone, not alcohol, to preserve the metachromasia. You
} can use various buffers to get the pH lower than 4 for even more selective
} staining.
} If you are staining epoxy sections, keep the tetraborate but eliminate the
} alcohol. Alcohol destroys metachromasia, at least in TB stained tissue.
}
} Jaclynn Lett wrote:
}
} } Regarding my Toluidine Blue stain solution, which should be staining animal
} } tissue varying shades of blue-purple (which I want), but is now staining
} } bright blue instead (which I don't want):
} }
} } 1% Toluidine Blue O
} } 1% Sodium Tetraborate Dodecahydrate
} } 30% Ethanol
} } in DDI
} }
} } (1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)
} }
} } I've received several suggestions to change the pH--some to acidic, some to
} } basic. Which direction should it be changed and what chemical do I use to
} } change it?
} }
} } Thank you again,
} }
} } Jaclynn M. Lett, Research Technician
} } Electron Microscopy Core Facility
} } Fay and Carl Simons Center for Biology of Hearing and Deafness
} } Central Institute for the Deaf
} } 4560 Clayton Ave.
} } St. Louis, MO 63110
} }
} } jlett-at-cid.wustl.edu
} } 314-977-0257
}
} Geoff
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************

--
John V Nailon
Executive Officer and Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld

From daemon Wed May 29 21:14:22 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hummer coaters usually have the rotary pumps installed in the console.

If you can remove the oil using a tube of some type: great.

If not, there are four nuts holding the pump down that can be easily
accessed from the bottom.

Once the nuts & the pumping hose on the top of the pump are removed the pump
can be easily removed for servicing.

Regards,

Earl

----- Original Message -----
} From: "Susan Carbyn" {CarbynS-at-EM.AGR.CA}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 29, 2002 11:59 AM

Regarding my Toluidine Blue stain solution, which should be staining animal
tissue varying shades of blue-purple (which I want), but is now staining
bright blue instead (which I don't want):

1% Toluidine Blue O
1% Sodium Tetraborate Dodecahydrate
30% Ethanol
in DDI

(1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)

I've received several suggestions to change the pH--some to acidic, some to
basic. Which direction should it be changed and what chemical do I use to
change it?

Thank you again,

Jaclynn M. Lett, Research Technician
Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

jlett-at-cid.wustl.edu
314-977-0257

From daemon Wed May 29 21:53:46 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear microscopists,

The page with microscopy Producers & Vendors at my Petr's Microscopy
Resources (http://www.petr.isibrno.cz/microscopy/production.php) has
been modified and extended.

New links (not only in Production & Sale category) will be
appreciated. If your URL (laboratory, company, congress, journal,
..,) isn't in the Petr's Microscopy Resources or if you have found an
interesting URL, please fill in the simple form at the
http://www.petr.isibrno.cz/microscopy/PMRform.php. Your submission
will be reviewed and included within one day.

Regards,

Petr Schauer

+--------------------------------------------------------------------+
| Dr. Petr Schauer tel.: (+420 5) 41514313 |
| Head of Electron Microscopy Laboratory fax : (+420 5) 41514404 |
| INSTITUTE OF SCIENTIFIC INSTRUMENTS (+420 5) 41514402 |
| ACADEMY OF SCIENCES OF THE CZECH REPUBLIC e-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno petr-at-dp.fce.vutbr.cz |
| Czech Republic www: http://www.petr.isibrno.cz/ |
+--------------------------------------------------------------------+

From daemon Thu May 30 05:09:34 2002

Contents Retrieved from Microscopy Listserver Archives
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Greetings All

If anyone has a spare or redundant PC1916 Computer Board for our ailing
Oxford eXLII system we would really like to hear about it.

I am told that there is one available at Oxford in the UK but the cost
is beyond us.

Our serial number for the board is 1490 - i1J
ass no. W - 0140276
PC1916 1128 - 184 - V3

Numbers for the system : Model no. 110 - 012
Serial no. 16112
ass no. W - 0140530

Thanks in anticipation.

Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website: www.nu.ac.za/microscopy.asp
Email: bruton-at-nu.ac.za
Postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa

From daemon Thu May 30 07:10:07 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (volfova-at-pobox.sk) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 30, 2002 at 04:10:19
---------------------------------------------------------------------------

Email: volfova-at-pobox.sk
Name: Volfova Petra

Organization: Faculty of Chemical and Food Technology,Slovak
University of Technology,Department Plastics and Rubber

Education: Graduate College

Location: Bratislava, Slovak republic

Question: I have got some problems with the staining of
polystyrene/poly(butyl acrylate) core/shell particles with OsO4 and
uranyl acetate UA combination. There are not contrast between core
and shell. When I used only either UA or OsO4, I obtained the same
particles without contrast. Only when I functionalized either core or
shell of particles for example carboxyl groups, there were contrast
between core and shell, if I used the UA only. I predict reactions
between -COOH and UA. But where I do the mistake? I thik the time of
staining is very important factor. What do you think?
Thank you very much.

---------------------------------------------------------------------------

From daemon Thu May 30 08:04:13 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,
sorry to go a bit off topic and stray into X-ray land, but I have an
offer (and a question) which I hope someone can help me with.

First, I have a microfiche set of 1987 JCPDS crystal data files (with
microfiche reader) which is free for anyone to have if they want it. Too
expensive to throw away. If you want it you will have to collect/pay for
someone to collect it for you.

Second, I am looking for crystallographic data on TiW and TiW(N), which I do
not have even in my more recent CD-ROM JCPDS data files. I would like
details of lines seen up to 85 degrees 2 theta with Cu Kalpha radiation. I
suspect that there is no data on this, but would prefer to be proved
wrong...

Many thanks,

Richard

_______________________________
Richard Beanland
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com

=======================================================================
This e-mail is intended for the person it is addressed to only. The
information contained in it may be confidential and/or protected by
law. If you are not the intended recipient of this message, you must
not make any use of this information, or copy or show it to any
person. Please contact us immediately to tell us that you have
received this e-mail, and return the original to us. Any use,
forwarding, printing or copying of this message is strictly prohibited.

No part of this message can be considered a request for goods or
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From daemon Thu May 30 08:20:20 2002

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lillianft-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 30, 2002 at 08:07:21
---------------------------------------------------------------------------

Email: lillianft-at-aol.com
Name: lillian Domenico

Organization: College of St. Elizabeth

Education: Undergraduate College

Location: City, State, Country Morristown, NJ

Question:
I will be teaching an introductory course on SEM in the Fall. I am
in the process of looking for simple procedures for tissue
preparations. Do you have any lab manuals you could recommend?
Thanks
Lillian Domenico

---------------------------------------------------------------------------

From daemon Thu May 30 10:54:45 2002

Contents Retrieved from Microscopy Listserver Archives
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A Philips EM400 STEM is available for sale in Riverside, CA.
It is a 24 years old instrument with W filament, �60� goniometer tilt, with
EDAX EDS system PV9100 and Si(Li) detector. The scope is not operational due
to minor vacuum problem. The V7 valve needs to be fixed or replaced.

We are open to discuss any offers.

Krassimir Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel 909 787 2998
Fax 909 787 4324

From daemon Thu May 30 11:46:44 2002

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Dear Susan,
We had an old Hummer for years, I think it was a Hummer I, and I recall that
the oil drain could have a small hose attached to drain the oil. Just drain
it all out and add new to the right place on the site glass. To "ballast"
the oil just means to run a bit of air through it to drive off all the water
vapour or other solvent vapours that might be in the fresh oil. Don't pump
on a wide open inlet, just a little air leak for about ten minutes to clean
the oil. I recall that my Hummer had an air leak valve that you could
control to bleed a little air into the chamber.
Hope this helps.
At 02:59 PM 05/29/2002 -0400, you wrote:
}
} Hi!
}
} We have a Hummer VII sputter coater, and I know the oil has not been
changed in many years. I wanted to change the oil, but have no instructions
on how to do this - and the person who was responsible for this machine is
now gone. The drain and fill points are obvious, but are there any things
that I need to be aware of before doing this? How do I go about ballasting
the oil? Please bear with the questions - as in the past - I was only a
user of the equipment - not someone who has experience in maintaining it.
}
} Thanks for any information!
}
} Susan
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca

From daemon Thu May 30 11:59:48 2002

Contents Retrieved from Microscopy Listserver Archives
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Volfova,

I recommend that you stain with ruthenium tetroxide. If my memory serves me
correctly, polystyrene is stained heavily with RuO4 whereas
poly(butylacrylate) is not. By comparison, OsO4 stains neither molecule
particularly well.

My recommendation is that you cut cryosections of the material and stain
these for several minutes in the vapors of an RuO4 solution. Allow the
stained sample to degas in the hood for a bit prior to handling and
microscopy. Alternatively, one might consider include staining the block
face for 30 minutes to several hours (just a guess on the staining
duration) followed by cryogenic sectioning for TEM. Only experimentation
will allow you to determine the best method for you materials. I also
recommend that you develop staining protocols on majority/minority blends
of known volume fraction. This ensures that you know beyond a doubt the
reactivity of the stain to each blend component. Once this is known, you
can attack the sample of concern with confidence in your ability to
properly interpret the results.

OsO4 and RuO4 stain by different mechanisms. Therefore, take care not to
overstain the material with RuO4. RuO4 may rapidly stain many materials in
a matter of minutes to hours whereas the same materials appear relatively
impervious to overstaining with OsO4 regardless of staining duration.

I wish you the best,

Gary M. Brown
phone: (281) 834-2387

volfova-at-pobox.s
k (by way of To: Microscopy-at-sparc5.microscopy.com
Ask-A-Microscop cc:
ist) Subject: Ask-A-Microscopist: staining of
polystyrene

05/30/02 06:59
AM

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (volfova-at-pobox.sk) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 30, 2002 at 04:10:19
---------------------------------------------------------------------------

Email: volfova-at-pobox.sk
Name: Volfova Petra

Organization: Faculty of Chemical and Food Technology,Slovak
University of Technology,Department Plastics and Rubber

Education: Graduate College

Location: Bratislava, Slovak republic

Question: I have got some problems with the staining of
polystyrene/poly(butyl acrylate) core/shell particles with OsO4 and
uranyl acetate UA combination. There are not contrast between core
and shell. When I used only either UA or OsO4, I obtained the same
particles without contrast. Only when I functionalized either core or
shell of particles for example carboxyl groups, there were contrast
between core and shell, if I used the UA only. I predict reactions
between -COOH and UA. But where I do the mistake? I thik the time of
staining is very important factor. What do you think?
Thank you very much.

---------------------------------------------------------------------------

From daemon Thu May 30 12:13:04 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Morning All,

For those among you who are wise, you should probably immediately
jump or GOTO END!

This is difficult to put into words without chancing a gross error
or causing some offence, but here goes anyway.

When we "fix" a specimen taken from a biological source, we NEVER
quite know what we are doing. The entire process is 'outcome-based'
(Ugh!!!). How does it look? Does the fixative 'coagulate' or 'cross-link'
the myriad of chemical entities within the sample? What is the effect of
specimen size? What about temperature? How long to kill? How long to
achieve minimum preservation? Preservation of what? What will constitute
comparable samples that permit statistical comparison of specimens of mouse
and elephant liver? We preserve or fix or kill or maintain color or get rid
of it. Behind every act there lies a reason, and in consequence of every
act there is produced an uncountable number of variables which only
REPRESENT what existED in the original, living, organism. Everyone says
that s/he already knows this!

Metachromasia is part physical chemistry, part biochemistry (and by
reason of those two, for many, little more than pure magic!). Metachromasia
has been extensively used, extensively studied and extensively reported
upon. Several items of information are clear [This may be an
exaggeration!].

1. The polychrome effect noted in the use of several related dyes
of the thiazine (quinone-imine/methylene blue) family is based on the
polymerization of dye molecules and the distances between adjacent acidic
groups on the substrate.

2. A loss of the polychrome effect could be attributed to*:
a. decrease in the concentration of some tissue/substrate
component
b. decarboxylation [- or - esterification with alcohol?]
c. degradation of the substrate to diffusible constituents.

[* see Pearse, A.G.E.(1985), Histochemistry, Theoretical and
Applied(4th Ed.), Vol II, Churchill Livingstone, NY,NY, pp701-710. ISBN:
0-443-02997-0.

3. In order for the metachromatic color. for (Toluidine Blue O,
that is purple [red + blue]) to be observed, the appropriately spaced acid
moieties must be deprotonated, i.e. negative in charge. Otherwise, the
orthochromatic color is observed.

In other words, in order for there to be metachromasia of a
particular component of the tissue, that component must have appropriately
spaced negatively charged groups.

4. At pH 7, most 'acid' moieties in biological systems are
deprotonated, and thus, negatively charged. The isoelectric points of most
macromolecules are in the vicinity of pH 5 [This IS an exaggeration!] Where
pH can determine dye binding, pH can be used to partition objects in the
specimen space along a pH gradient [also in Pearse, same pages, but see
Methylene Blue Extinction (MBE) methods in many compendia]. [MBE used to
distinguish among histologic 'acid' mucosubstances (GAG's, etc.).]

5. A buffer may stabilize substances, react with them, or promote
changes in them (i.e. oxidation).

6. A bottle of dye, used for 5 years to produce a result, may, at
some moment, STOP behaving as it should. Pearse mentions that one of the
reasons that metachromasia was such a confused subject prior to the 50-60's,
was due to the fact that so many studies failed to use pure dyes.

RULE: If a dye fails to function as it should. Try a different batch or
make up a fresh batch, or purchase a new supply. I have found that a simple
0.1% solution of the dye gives rise to regular, reproducible metachromasia
which survives dehydration in absolute ethanol, but never in 95% ethanol.
That having been said, when I have performed the Azure B, pH 4.0 for
ethanol-acetic acid(3:1)(Clark) fixed nucleic acids (Flax and Himes, 1952),
I follow their protocol for dehydration and use tertiary butanol.
Preparations I made in the mid-60's still show metachromasia, albeit with
some overall loss of color in my personally prepared Damar-xylene mountant.

The questions about the failure of any regularly used dyeing
procedure amount to a scientific challenge that are best addressed by the
one who is having the problem. Since the variables are many, the sources of
failure are also many. One has to learn how to perform a component analysis
in order to efficiently address such a problem. Since much of what one does
in dyeing/staining is by prescribed protocol, it should be clear that if
anything has changed, it is the operator who is in the best position to DO
the troubleshooting. When I ran the Flax and Himes procedure, I always
retained the blocks of previously sectioned material. Each sectioned block
was dipped in paraffin to cover the exposed tissue and stored carefully
away. I was especially careful of those specimens, prepared for any
particular purpose, in the event I ever required a 'known' tissue source of
a good result. Even so, I was aware that the stored specimens would not be
the same in two years as those I sectioned yesterday. I learned this when I
addressed the issue of saving tissues labeled with tritiated thymidine.
Why, I asked parenthetically, should I be able to determine that loss of
radioactivity by disintegration was not going to be augmented by loss due to
progressive destruction of DNA, if I had no specific knowledge of how much
DNA/nucleus/section was present in the starting material? So, I learned
that I would not be able to have compete faith, even in the best of my
archived specimens, 5 or 10 years in the future.

NOTE: Acridine orange was one of the first fluorescent dyes used by
virologists in the late 50's/early 60's. One was able to distinguish
between single stranded RNA and double-stranded DNA until someone noted the,
then recent, discovery of the RNA viruses. "Nuts!" Even now, there is an
extensive literature on the use of AO fluorescence for double-dyeing the
nucleic acids in cell nuclei.

An absolute obligation of old windbags is a SUMMARY: If there is a
single point in all of this, it is this. In the application of
metachromasia there are considerations of mass action, pH, purity of the
dye, and treatments preceding and following the application of the dye. If
each adds an order of magnitude to the number of variables involved, there
are 4-5 such ordinal magnifications through the process. If the physical
and chemical bases of histologic methods are understood at less than optimal
levels, then troubleshooting will be a problem that has little hope for
success. On the other hand, even one who has only the recipe to which s/he
can refer can be taught component analysis of that recipe.

Mom used to say, "If you don't know what kind of flour to put in
your bread, try any flour and the bread will let you know if you were right
or wrong. If you were wrong, and really want bread, then you will have to
try another flour, and another, until the bread tells you that you are
finally right. Just don't change any other part of the recipe while you are
testing the flour." Ah! If only more of us had learned to bake when we
were young.

END: Respectfully submitted,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

From daemon Thu May 30 12:14:38 2002

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Afternoon Susan,
I think you can read the information you want at the following site.
It's simple, straight forward, and I couldn't find anything wrong with the
procedure to replace the oil.

URL: http://safescience.ucsc.edu/Equipinst/vacpump.html

You might also want to know that I was recently given a nice pump that
hadn't been used for a while. Along with it came a case of oil and a case
of what was called Pump Flush. I used 4 quarts of the flush before the
cleaning was done. You might expect the same from small amounts of oil.

If you are interested in the flushing fluid, contact Savant Industries at
1-800-634-8886, and ask for information on "Savant Vacuum Pump flushing
Fluid", catalog No., SFF-1. I think that it works REALLY well.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

} ----------
} From: Susan Carbyn
} Sent: Wednesday, May 29, 2002 2:59 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Maintenance question
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi!
}
} We have a Hummer VII sputter coater, and I know the oil has not been
} changed in many years. I wanted to change the oil, but have no
} instructions on how to do this - and the person who was responsible for
} this machine is now gone. The drain and fill points are obvious, but are
} there any things that I need to be aware of before doing this? How do I
} go about ballasting the oil? Please bear with the questions - as in the
} past - I was only a user of the equipment - not someone who has experience
} in maintaining it.
}
} Thanks for any information!
}
} Susan
}
}
}
} Susan Carbyn
} Electron Microscopy Technician
} Atlantic Food and Horticulture Research Centre
} Agriculture and Agri-Food Canada
} 32 Main Street, Kentville, N.S. B4N 1J5
} Canada
}
} Phone (902) 679-5535
} Fax (902) 679-2311
}
} E-Mail: carbyns-at-em.agr.ca
}
}
}

From daemon Thu May 30 12:31:47 2002

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Folks;

Has anyone found an optical microscopy solution to measuring microelectronic
bond wire loop height and length in one instrument? Our problem is that we
want to measure the length of a wire [.001" diameter] that has been formed
into a loop connecting two points in a ckt. This is generally not a
critical measurement but at our frequency range small variations in this
formation can mean large electrical parasitic effects.

Regards,

Peter Tomic
Anadigics, Inc.

From daemon Thu May 30 13:48:42 2002

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Greetings:

Our near and dear to my heart ETEC autoscan is going to be replaced this
summer by a more up to date SEM. The ETEC is in working order and comes
with a complete set of spare parts.

Please contact me directly if interested. The scope is located in NE Iowa.

Cheers,

Robert

Robert Fitton
Teaching Associate/Director of Laboratories
Luther College
Department of Biology
700 College Drive
Decorah, IA 52101

Voice 563-387-1559
FAX 563-387-1080

From daemon Thu May 30 14:47:04 2002

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Hello,
We will be de-commissioning a Neuron Tracing System, by Eutectics
Electronics, available to anyone who can make use of it, or who might
need it for parts. It is fully functional.

Free to a good home for the cost of shipping.

HP 2-pen plotter, lots of pens
Gateway 80486 computer, with monitor
Mark 4 stage with joystick and controller, extra old-style joystick and
stage motors
4 K vector graphics processor
Vector graphics display
Focus drive
NTS software version 5
sorry, we are keeping the venerable Zeiss WL microscope.

Regards,
Glen

--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************

From daemon Thu May 30 18:21:43 2002

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Fred

I don't understand your point about Acridine Orange (AO)? How discovery of
the RNA-viruses affects the staining and our knowledge about this
particular staining agent? As I remember, AO staining is based on polar
interactions, mostly with "nucleic acids". The 'color' of fluorescence
depends from the energy transfer efficiency between donor (AO) and acceptor
(which may be, may be not nucleic acid). I expect, the 'color' from AO
stained double strands RNA would be similar to DNA. Another point here, as
far as I know, RNA viruses in most cases contain "double-stranded RNA
segments", so it's a mixture single/double-stranded RNA. I could not
predict how it will fluorescence with AO. Nevertheless, such viruses are
very small and I don't believe you could even see their fluorescence with
AO on the autofluorescence background of the typical cell. The bottom line
here: the discovery of the new "RNA-viruses" is not affected our knowledge
of basic properties of AO and AO would work (I believe) at the same manner
with this 'double-stranded RNA' as with other stuff. I don't see big
difference between single/double-stranded-RNA and DNA. It's well known,
that most RNAs tends to form secondary structure (call it double-stranded
RNA fragments) under the physiological conditions. Moreover, my personal
experience shows that it's extremely difficult to make RNA completely
'single-stranded': even at the presence of 8MUrea+formamide,

Sergey

At 09:56 AM 5/30/02, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From daemon Thu May 30 20:04:02 2002

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Could anyone respond with typical uptime for
the Philips XL-30 high vac system? This is the
FESEM version. At issue are multiple users,
stupid things that can happen, cost of the stupid
things, frequency thereof, etc.

would appreciate all feedback. Private off-line is
preferred.

tnx,
gary g.

From daemon Thu May 30 21:06:38 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Status:
}
} Hi,
}
} A colleague asked an interesting question the other day: were there
} any legal requirements for microscope images?
}
} The only issues which I have seen are the following:
} 1. The standard format set by MSA is TIFF.
} 2. Ethically, an image can be processed for improved publication but
} not to any extent which corrupts data. Better microscopy is
} strongly preferred over processing.
}
} Do any of you know of any other legal ramifications?
}
} Thanks in advance for any input.
}
} Best regards,
} Barbara Foster
} Microscopy/Microscopy Education
} 125 Paridon Street, Suite 102
} Springfield, MA 01118
} PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
}

From daemon Fri May 31 05:59:20 2002

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Dear All,
Can anyone please point me in the direction of easily
understandable information on the nature of Moire
fringes. I have a good article from Scientific
American dated 1963(!), but have almost no other
information.
I have tried Google and a search, but need to sort out
the good from the bad/misleading. Any leads would be
much appreciated.
Thanks, Jeremy
jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com

From daemon Fri May 31 06:27:01 2002

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Processing is not ethical if it corrupts data?? Hmm--- strictly, any
processing *at all* corrupts the primary image data.
Most people would accept a bit of sharpening or noise reduction or
contrast enhancement as part of the normal image preparation
process - except of course where the absolute values of pixels are
important, as in ratio imaging for example. Even conventional silver
photographers do this routinely, by choosing a development
protocol and printing paper grade to optimise image contrast, or by
dodging or holding back during printing, or by spotting out dust and
scratches. Even some sharpening or blurring was possible using
conventional non-digital technique, by choosing appropriate
condensors in the enlarger, etc. The names of the digital
equivalents of these manipulations derive from their applications in
silver imaging - viz. the dodging tool and unsharp mask sharpening
in Photoshop, so there is nothing new about a bit of image
processing in the interests of optimal data presentation.

Furthermore, what is the status of image processing techniques for
noise reduction such as image averaging / integration, fourier
transformation / summation / filtration / reconstruction. What about
ratio and subtraction images - are these to be regarded as corrupt
data too? Most people regard these as techniques for data
acquisition and enhancement rather than data corruption, and thus
the end-process of the image processing IS the original primary
image data.

So another definition of corruption of data is required. In my view,
the issue should not be whether the image has been processed or
not. If there is a role for a legal definition at all, the question should
be "at what point does processing cross the line (if there is such a
thing) into image manipulation intended to misrepresent or
mislead?". My guess is that it will always be very hard to provide a
single answer to that, as to so many other legal questions. Why
else do we have juries?

Chris

} } Hi,
} }
} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is
} } strongly preferred over processing.
} }
} } Do any of you know of any other legal ramifications?
} }
} } Thanks in advance for any input.
} }
} } Best regards,
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
}

==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Waddington Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JN, Scotland, UK
Tel. #44 (0) 131 650 5554
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================

From daemon Fri May 31 07:12:35 2002

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My .02....

Typically, the ultimate vacuum achieved will be reduced while the ballast
valve is open. Vacuum should improve on closure.

I back-fill my coaters (as well as the SEM) with the off-gas from my liquid
nitrogen cryo. My vacuum pump oil must be treated as both radioactive and a
hazmat, therefore changes are expensive and infrequent. Given this reality,
I "ballast" the pumps often using the dry N2. I have not done a study to
quantify the improvement by using dry N2 in "flushing" the oil of
contaminants, but it should be more effective than air (especially in humid
environments).
_W_

Woody White
McDermott Technology Inc.
McD: http://www.rdd.mcdermott.com/
Mine: http://woody.white.home.att.net

-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Thursday, May 30, 2002 12:39 PM
To: Susan Carbyn
Cc: Microscopy-at-sparc5.microscopy.com

Dear Susan,
We had an old Hummer for years, I think it was a Hummer I, and I recall that
the oil drain could have a small hose attached to drain the oil. Just drain
it all out and add new to the right place on the site glass. To "ballast"
the oil just means to run a bit of air through it to drive off all the water
vapour or other solvent vapours that might be in the fresh oil. Don't pump
on a wide open inlet, just a little air leak for about ten minutes to clean
the oil. I recall that my Hummer had an air leak valve that you could
control to bleed a little air into the chamber.
Hope this helps.
At 02:59 PM 05/29/2002 -0400, you wrote:
}
} Hi!
}
} We have a Hummer VII sputter coater, and I know the oil has not been
changed in many years. I wanted to change the oil, but have no instructions
on how to do this - and the person who was responsible for this machine is
now gone. The drain and fill points are obvious, but are there any things
that I need to be aware of before doing this? How do I go about ballasting
the oil? Please bear with the questions - as in the past - I was only a
user of the equipment - not someone who has experience in maintaining it.
}
} Thanks for any information!
}
} Susan
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca

From daemon Fri May 31 07:41:15 2002

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Barbara;

With respect to legal requirements of images, and by that I assume you mean
ownership, I would suggest reviewing copyright laws that apply. We often ask
our clients/customers to sign NDA's [Non-Disclosure Agreements] since many
of our analytical reports contain proprietary information and images.

I would also suggest checking with some forensic people since images,
electronic or otherwise, in criminal investigations is critical. There was
someone at an MSA conference in Monterey, CA from FBI back in ~ 1993 that
gave a good talk on this subject.

Hope this helps some.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: Ask-A-Microscopist [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Thursday, May 30, 2002 9:56 PM
To: Microscopy-at-sparc5.microscopy.com

} Status:
}
} Hi,
}
} A colleague asked an interesting question the other day: were there
} any legal requirements for microscope images?
}
} The only issues which I have seen are the following:
} 1. The standard format set by MSA is TIFF.
} 2. Ethically, an image can be processed for improved publication but
} not to any extent which corrupts data. Better microscopy is
} strongly preferred over processing.
}
} Do any of you know of any other legal ramifications?
}
} Thanks in advance for any input.
}
} Best regards,
} Barbara Foster
} Microscopy/Microscopy Education
} 125 Paridon Street, Suite 102
} Springfield, MA 01118
} PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
}

From daemon Fri May 31 07:51:33 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Barbara,
A number of years ago I had a customer in a state crime lab and he told
me that any digital images had to be captured on a WORM drive (Write
Once, Read Many), now CD-R. This was considered the rough equivalent of
a photographic negative in court.

Also, there were several threads over the past couple of years about
enhancing (altering) data. I believe the main thrust was that you had
to have the original data and also be able to describe (preferably with
equations) what was done to alter the data to its final state. The
threads go into much more detail.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Status:
} }
} } Hi,
} }
} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is strongly
} } preferred over processing.
} }
} } Do any of you know of any other legal ramifications?
} }
} } Thanks in advance for any input.
} }
} } Best regards,
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
}
}

From daemon Fri May 31 08:17:58 2002

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Peter,
We have an optical based micrometer attached to our Zeiss Axiophot and
Axiovert stage. this gives Z in 1 micron increments. The units are made by
Boeckeler Instruments who did the modification / attachment. The sensitivity
will of course depend on the quality of the stage drive.
Russ Gillmeister
Xerox
~~~~~~~~~~~~~

-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
Sent: Thursday, May 30, 2002 1:30 PM
To: Microscopy Listserver (E-mail)

Folks;

Has anyone found an optical microscopy solution to measuring microelectronic
bond wire loop height and length in one instrument? Our problem is that we
want to measure the length of a wire [.001" diameter] that has been formed
into a loop connecting two points in a ckt. This is generally not a
critical measurement but at our frequency range small variations in this
formation can mean large electrical parasitic effects.

Regards,

Peter Tomic
Anadigics, Inc.

From daemon Fri May 31 09:10:46 2002

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To all,

There is a typographical error in the meeting notice for the Philadelphia
Society of Microscopy notice for our meeting at Longwood Gardens on June
6th. The email address should read

jreffner-at-rohmhaas.com

We put too many r's in the notice. Alternatively, you can simply reply to
me at this email address.

Our apologies for the inconvenience.

Robert A. Carlton
Elan Drug Delivery, Inc.
3500 Horizon Drive
King of Prussia, PA, 19406
610-313-1360
robert.carlton-at-elan.com

********************************************************************
This communication and any files transmitted with it
may contain information that is confidential, privileged
and exempt from disclosure under applicable law.
It is intended solely for the use of the individual or entity
to which it is addressed. If you are not the intended
recipient, you are hereby notified that any use,
dissemination or copying of this communication is strictly
prohibited. If you have received this communication in
error, please notify the sender. Thank you for your co-operation.
********************************************************************

From daemon Fri May 31 09:28:47 2002

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Have you seen.....

Reyes-Gasga J., Tehuacanero S., and Yacaman M. Jose. 1998. Moire
Patterns in High Resolution Electron Microscopy Images of MoS2.
Microsc. Res. Tech. 40:2-9, 1998.
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================

Jeremy Sanderson wrote:

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-----------------------------------------------------------------------.

Dear All,
Can anyone please point me in the direction of easily
understandable information on the nature of Moire
fringes. I have a good article from Scientific
American dated 1963(!), but have almost no other
information.
I have tried Google and a search, but need to sort out
the good from the bad/misleading. Any leads would be
much appreciated.
Thanks, Jeremy
jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com

From daemon Fri May 31 11:17:35 2002

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This thread seems to reflect a common phenomenon that accompanies
technological progress: in effect, that when a more objective and
quantifiable means of accomplishing something becomes possible, it is held to
a higher standard than the technology or prior art that preceded it. For
example, DNA evidence has been subjected to a level of quantified review that
fingerprinting has generally not. Newly synthesized drugs are routinely
evaluated prior to legalization in ways that botanicals have seldom been
scrutinized.

Since digitized images are readily subject to quantifiable changes that, as
Ken says, may be described numerically, it doesn't strike us as odd to suggest
that changes made to them should be scrutinized in that way. And yet every
step in conventional photography is susceptible to substantial variations, the
sum of which is probably beyond quantifying. (Imagine! One is steeping a
sheet of film in chemical after chemical in an attempt to produce a stain that
resembles something seen by the eye!)

What of that hallowed original negative? I wonder how often photographic
evidence has been subjected to a rigorous review of how each step in its
storage and development affected its response (log e) characteristics,
contrast, density range, etc. And what about color?!. How long did time and
storage temperature act on the silver halide grain ripening? Were water
quality, time, temperature and agitation quantified at each step of
development, rinsing and fixing? What was the age of each of the baths and
the number of prior images processed at the time of this development? Was the
image tested for residual fixer or halide? How has this stained piece of
plastic and gelatin been stored since? How much highlight fading has it
undergone as a result of sulfur-containing gases and peroxides acting on the
image silver? Now, if dealing with a print instead of a negative, simply
repeat each of these variables and add them to the calculation. Next, if
there was intentional manipulation of the development in order to increase the
capabilities of the film, quantify that.

Leaving aside the enormous variations in color stability among different film
types, what of the inherent ability of dyes and pigments to render color? As
every critical photographer knows, for every film / print combination there
are certain colors that cannot be rendered accurately. Similarly, the means
by which manufacturers bias the intrinsic sensitivities of their CCD chips to
render an approximation of what the eye sees are not perfect (nor accessible
to the user, as they often form the basis for proprietary advancements in
their equipment.)

Along with prompting a review of what may be deemed acceptable within the
potentials of new imaging technology these advances should, perhaps, provoke
some thought about what the real level of accountability has been in regard to
the older technology.

John Twilley
Conservaton Scientist

Ken Converse wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Barbara,
} A number of years ago I had a customer in a state crime lab and he told
} me that any digital images had to be captured on a WORM drive (Write
} Once, Read Many), now CD-R. This was considered the rough equivalent of
} a photographic negative in court.
}
} Also, there were several threads over the past couple of years about
} enhancing (altering) data. I believe the main thrust was that you had
} to have the original data and also be able to describe (preferably with
} equations) what was done to alter the data to its final state. The
} threads go into much more detail.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} Delta, PA
}
} Ask-A-Microscopist wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } } Status:
} } }
} } } Hi,
} } }
} } } A colleague asked an interesting question the other day: were there
} } } any legal requirements for microscope images?
} } }
} } } The only issues which I have seen are the following:
} } } 1. The standard format set by MSA is TIFF.
} } } 2. Ethically, an image can be processed for improved publication but
} } } not to any extent which corrupts data. Better microscopy is strongly
} } } preferred over processing.
} } }
} } } Do any of you know of any other legal ramifications?
} } }
} } } Thanks in advance for any input.
} } }
} } } Best regards,
} } } Barbara Foster
} } } Microscopy/Microscopy Education
} } } 125 Paridon Street, Suite 102
} } } Springfield, MA 01118
} } } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} } }
} }
} }

From daemon Fri May 31 14:00:40 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, All-

The Education Committee of the Microscopy Society of America has recently
formed a new subcommittee on the ethics of digital image manipulation,
which intends to promote discussion of this issue. At M&M2002 in Quebec
City I'll be bringing it up during the Problem Solving with the Experts
session, which is scheduled for Tuesday at 8:30 am.

Currently, as far as I know, there are no legal or ethical standards for
digital image presentation except in clinical and forensic fields where
images may be used in legal cases. If anyone knows of any other standards,
please let me know.

I have some opinions and ideas, and I had planned to bring it up in this
list soon so I could get an idea how all of you felt, and then summarize
and invite more discussion at the meeting. I welcome any and all input!

I'll have a lot more to say as soon as I have time to write, but this is
as good a time as any to let the debate begin!

} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is
} } strongly preferred over processing.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

From daemon Fri May 31 14:00:42 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jeremy,
I just did a google on this. Second item at the top of the search list:
http://don.sci.mu.edu/classes/L1980210.htm

{- it's about gratings but for crystals it's the same principle. Moire fringes are interference patterns produced by two overlapping lattices or gratings.

Hope this helps,

Max
__________________________________
Max Sidorov
Materials Technology Development
Advanced Micro Devices

max.sidorov-at-amd.com

-----Original Message-----
} From: Jeremy Sanderson [mailto:jb_sanderson-at-yahoo.com]
Sent: Friday, May 31, 2002 3:49 AM
To: Microscopy-at-sparc5.microscopy.com

Dear All,
Can anyone please point me in the direction of easily
understandable information on the nature of Moire
fringes. I have a good article from Scientific
American dated 1963(!), but have almost no other
information.
I have tried Google and a search, but need to sort out
the good from the bad/misleading. Any leads would be
much appreciated.
Thanks, Jeremy
jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
http://uk.my.yahoo.com

From daemon Fri May 31 14:12:22 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The real test is that the individual that is introducing the photo must be able to testify that the image "truly and accurately depicts what it is purported to represent." That is the question that we are asked when a photo is being introduced in court. If you can testify in court that it does so it will probably be introduced. Even an image taken with a wide angle or telephoto lens does not meet this criteria if being introduced to show size relative to distance, but would for some other uses.

A lot of people have developed rules they believe the courts would want, like the one stated here, but I am unaware of any standard that has been set down by the courts themselves (appellate or above in published opinions) other than the "accurately depicts" test. If your testimony were challenged on that issue it would be of great help to have an original that you could prove had not been altered in any way. Our department is going to a great deal of trouble to set up a system that provides this. But introduction of the image does not require that, at least with-in the jurisdictions I've worked in.

Jim

James L. Roberts
Supervising Forensic Scientist
Comparative Analysis Section
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 654-2308

James.Roberts-at-mail.co.ventura.ca.us

} } } "Ken Converse" {qualityimages-at-netrax.net} 05/31/02 05:44AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Barbara,
A number of years ago I had a customer in a state crime lab and he told
me that any digital images had to be captured on a WORM drive (Write
Once, Read Many), now CD-R. This was considered the rough equivalent of
a photographic negative in court.

Also, there were several threads over the past couple of years about
enhancing (altering) data. I believe the main thrust was that you had
to have the original data and also be able to describe (preferably with
equations) what was done to alter the data to its final state. The
threads go into much more detail.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Status:
} }
} } Hi,
} }
} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is strongly
} } preferred over processing.
} }
} } Do any of you know of any other legal ramifications?
} }
} } Thanks in advance for any input.
} }
} } Best regards,
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
}
}

From daemon Fri May 31 14:40:31 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are looking to purchase a digital camera system for our TEM and
would like some feedback from those of you who already utilize such systems.
Some questions I'd like to ask are:

* What make & model camera system do you have?
* What type, make & model printer(s) do you use, (e.g. inkjet,
dye-sublimation, silver halide)?
* Do you feel you get micrograph-quality resolution and images
utilizing that system?
* Ease of use--is it user-friendly or cumbersome?
* Ease and/or quality of service--are any of the components
serviceable by you? How quickly & easily is it to get a service rep?
* How long have you had this system?
* Would you recommend this system--why or why not?
* What, if anything, might you have done differently?

I'd greatly appreciate any responses. Thank-you in advance for your
help.

Donna R. Clarkson

From daemon Fri May 31 15:44:36 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

James Roberts wrote:
=====================================================
The real test is that the individual that is introducing the photo must be
able to testify that the image "truly and accurately depicts what it is
purported to represent." That is the question that we are asked when a
photo is being introduced in court. If you can testify in court that it does
so it will probably be introduced. Even an image taken with a wide angle or
telephoto lens does not meet this criteria if being introduced to show size
relative to distance, but would for some other uses.

A lot of people have developed rules they believe the courts would want,
like the one stated here, but I am unaware of any standard that has been set
down by the courts themselves (appellate or above in published opinions)
other than the "accurately depicts" test. If your testimony were challenged
on that issue it would be of great help to have an original that you could
prove had not been altered in any way. Our department is going to a great
deal of trouble to set up a system that provides this. But introduction of
the image does not require that, at least with-in the jurisdictions I've
worked in.
==========================================================
One must always remember that the expert giving such testimony is rendering
professional judgements and opinions and if one or another party to a
dispute should believe or try to construe that the independent expert made a
professional error, which resulted in economic loss to them, then that
expert can be sued for professional malpractice just as a physician or any
other professional can be sued in our courts by anyone who feels they have
been harmed by the professional negligence of the expert.

This reality becomes a powerful force to make sure sure that any image
enhancement or alteration is done in ways that do not distort the outcome of
one's conclusions. Remember,just as for physicians and lawyers, one does
not really have to have made a profesisonal error, since someone needs only
to construe that an error was made, for them to be on the receiving end of a
law suit.

We all know that lawyers and physicians pay a great deal of money for
insurance to fund such losses. But people rendering opinions on microscopy
results tend to forget about the need for such insurance coverage, even
though many times they put their own personal assets at risk when they
render such testimony (without professional liablity insurance coverage).

Do laboratories get sued? You bet. Anyone doing this type of work should
have some kind of a program of loss prevention and risk analysis in order to
reduce the risk that any of their laboratory's results could be
misinterpreted or misconstrued by others.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================

From daemon Fri May 31 16:02:41 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Everyone,

We are using an Hitachi S-570 SEM for examination of rock thin sections
mounted on 1" x 3" glass microscope slides. Up until now, we have been
securing the slides to a large circular (50 mm) specimen holder with
copper and/or carbon tape. We would like a holder that would not require
the use of tape, carbon paint, etc. Does anyone know if there are any
commercially available holders (similar, for example, to that found in a
Cameca Microprobe, where a metal frame secures and grounds the slide to
the base) that would work in our microscope?

Any design ideas would also be appreciated since our machine shop will
fabricate what we need as long as nothing is commercially available.

Thanks for any help,

Heather Owen

Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall, P.O. Box 413
Milwaukee, WI 53210
USA

Phone: (414)229-6816

From daemon Fri May 31 17:29:54 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

{fontfamily} {param} Times_New_Roman {/param} Cerius users: 5/31/02

{/fontfamily} {fontfamily} {param} Times {/param}

I am having troubles with image simulations along low-index
orientations using Cerius program (Cerius 2 Ver.4).

I redefined unit-cell and changed the orientation that I want to
calculate to a primary axis. I divided the new cell into several slices
and I calculated projected potential for each slice. However, the
resulting ED patterns have extra spots that dose not match to the
reciprocal lattice points of the original unit-cell. I thought the
extra spots are from high order Laue zone, but the spacing does not
match to that I expect in some cases.

What I would like to ask are:

(1) How can I interpret the calculated ED patterns, assuming that
Cerius does not go wrong?

(2) If Cerius code has troubles, at what conditions does it go wrong?

(3) Is there any other programs that allow us to calculate low-index
orientation images?

The msi files of the structures, log files, and the calculated
diffraction patterns, and copies of comments I received from a user and
the developer are available upon request.

I would appreciate any comments you might have.

Hiromi konishi hkonishi-at-asu.edu {smaller}

{/smaller} {/fontfamily}

From daemon Fri May 31 18:10:45 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

The real test is that the individual that is introducing the photo must be
able to testify that the image "truly and accurately depicts what it is
purported to represent." That is the question that we are asked when a
photo is being introduced in court. If you can testify in court that it
does so it will probably be introduced. Even an image taken with a wide
angle or telephoto lens does not meet this criteria if being introduced to
show size relative to distance, but would for some other uses.

A lot of people have developed rules they believe the courts would want,
like the one stated here, but I am unaware of any standard that has been
set down by the courts themselves (appellate or above in published
opinions) other than the "accurately depicts" test. If your testimony were
challenged on that issue it would be of great help to have an original that
you could prove had not been altered in any way. Our department is going to
a great deal of trouble to set up a system that provides this. But
introduction of the image does not require that, at least with-in the
jurisdictions I've worked in.

Jim

James L. Roberts
Supervising Forensic Scientist
Comparative Analysis Section
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 654-2308

James.Roberts-at-mail.co.ventura.ca.us

} } } "Ken Converse" {qualityimages-at-netrax.net} 05/31/02 05:44AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Barbara,
A number of years ago I had a customer in a state crime lab and he told
me that any digital images had to be captured on a WORM drive (Write
Once, Read Many), now CD-R. This was considered the rough equivalent of
a photographic negative in court.

Also, there were several threads over the past couple of years about
enhancing (altering) data. I believe the main thrust was that you had
to have the original data and also be able to describe (preferably with
equations) what was done to alter the data to its final state. The
threads go into much more detail.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Status:
} }
} } Hi,
} }
} } A colleague asked an interesting question the other day: were there
} } any legal requirements for microscope images?
} }
} } The only issues which I have seen are the following:
} } 1. The standard format set by MSA is TIFF.
} } 2. Ethically, an image can be processed for improved publication but
} } not to any extent which corrupts data. Better microscopy is strongly
} } preferred over processing.
} }
} } Do any of you know of any other legal ramifications?
} }
} } Thanks in advance for any input.
} }
} } Best regards,
} } Barbara Foster
} } Microscopy/Microscopy Education
} } 125 Paridon Street, Suite 102
} } Springfield, MA 01118
} } PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
} }
}
}

From daemon Fri May 31 18:14:29 2002

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