I hope this doesn't start off a thorny side debate about the pros and cons of turning our microscopes off or not when not in use, but I think that Ritchie is correct. I don't know your local situation but unless there is some really compelling reason to turn the electron optics console off on weekends then you should ideally leave it on permanently as you do the vacuum system. (Just blank out or turn down the brightness if you are worried about "burn marks" from characters on the TV monitor screens?) There are at least two elements to this:
1. Even if leaving the electronics on all of the time means that you have to replace the monitor screens every few years, the risk of other much more serious (read expensive) things going wrong over the microscope's service life as a result of cycling the power hundreds of times, is probably a lot more significant. The replacement costs for the monitor screens would generally be peanuts compared with the costs of diagnosing and replacing some of the other dozens of bits and pieces in the electronics console, especially any one of a large number of individual boards that could fail. Add to that the cost of the down time while you wait for replacement parts. Usually you get a fair bit of warning from an ageing monitor screen via gradual loss of picture quality, so you can change it at leisure, and replacement costs should only be in the order of hundreds of dollars or less rather than the all-up costs in the thousands of dollars or more for many components in the rest of the machine.
2. In any case there is a risk that turning the electron optics console on and off hundreds of times could abruptly shorten the life of the monitor tubes anyway -- even more than simply leaving them on, because thermal cycling of the filament will place mechanical stresses on the filament itself, the other gun components, and metal-glass seals etc.
I suppose the disclaimer is that of course the optimum on/off regime is dependent on one's particular local situation.
} } Reading you question ,Ritchie, I thought you might be right. The } electronics probably would last longer if I don't turn on and off the } console 50 times a year, but I think TV monitors, would burn out. } } Regards, } Pavel
Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457 Ansto Materials Division Fax: 61-2-9543-7179 PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au Australia www: http://www.ansto.gov.au/
Listers, I am interested in recirculating chillers that can be attached to electropolishing units. These 'chillers' pump coolant to the electropolishing unit and the temperatures range that I require needs to be as far down as -40°C. If someone has one could they send me the brand/make?
Listers, I am interested in recirculating chillers that can be attached to electropolishing units. These 'chillers' pump coolant to the electropolishing unit and the temperatures range that I require needs to be as far down as -40°C. If someone has one could they send me the brand/make?
Thanks in advance, Steven
-- Dr. Steven Celotto Department of Engineering Materials Science & Engineering University of Liverpool Brownlow Hill Liverpool L69 3BX UNITED KINGDOM
I booked my flight right into Quebec City. Check your arrangements. Montreal is a long way from Quebec City.
Lee
At 8:36 AM -0400 6/28/02, Corazon D. Bucana wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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I have been using the older Leica Lynx tissue processor (Now distributed by Electron Microscopy Sciences under the name Vision EMS processor) for 6 years and have been extremely pleased with the reliability, efficiency, and quality. There are a few potential short falls inherent with any automated tissue processor versus processing by hand which include the incorporation of air bubbles which can get trapped in the processing baskets and prevent good fluid exchange with the tissue. Also there is the potential problem of vials jamming if the vials are not seated securely in the carousel or if the vials are not symetrically round. I have not experienced jamming vials but I know this can be a problem if you are not careful. The automated tissue processors are extremely helpful in our lab because we process thousands of samples a year and the overall sample preservation is good.
David Cugier Associate Cellular and Molecular Biologist Abbott Labs 847-938-6725 David.Cugier-at-Abbott.com
kwalters {kwalters-at-blue.weeg. To: Microscopy-at-sparc5.microscopy.com uiowa.edu} cc: Subject: Lynx el Tissue Processor for EM samples 06/27/02 04:02 PM
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Hi,
Has anyone had experience with this automated processor of EM samples? Thanks for any information you can provide me.
The key to having command of any tool is to understand how it works! The microscope IS a tool!
Regards,
Fred Monson
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center West Chester University South Church Street and Rosedale West Chester, Pennsylvania, USA, 19383 Phone: 610-738-0437 FAX: 610-738-0437 fmonson-at-wcupa.edu CASI URL: http://darwin.wcupa.edu/casi/ WCUPA URL: http://www.wcupa.edu/ Visitors URL: http://www.wcupa.edu/_visitors/
} ---------- } From: danthony38-at-cogeco.ca } Sent: Friday, June 28, 2002 9:04 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Ask-A-Microscopist: Polarizer and Crystals in LM } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (danthony38-at-cogeco.ca) from } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday, } June 26, 2002 at 13:32:34 } -------------------------------------------------------------------------- } - } } Email: danthony38-at-cogeco.ca } Name: don anthony } } Education: 9-12th Grade High School } } Location: peterborough ont canada } } Question: I have seen beautiful photos of, (as I was told)chemicals } crystalized on glass slides. As it was explained to me, a polorizer } was placed in front of the light source,and a second polorizer, above } the crystals being photographed. The profusion of colour was } unbelieveable, and when the polorizer was moved slightly, the colours } changed, creating a different picture. My question is,after trying to } duplicate this proceedure, I'm getting no where, can anyone help? } Also what chemicals make for the best crystals. Many thanks Don } } -------------------------------------------------------------------------- } - } }
I have been using the older Leica Lynx tissue processor (Now distributed by Electron Microscopy Sciences under the name Vision EMS processor) for 6 years and have been extremely pleased with the reliability, efficiency, and quality. There are a few potential short falls inherent with any automated tissue processor versus processing by hand which include the incorporation of air bubbles which can get trapped in the processing baskets and prevent good fluid exchange with the tissue. Also there is the potential problem of vials jamming if the vials are not seated securely in the carousel or if the vials are not symetrically round. I have not experienced jamming vials but I know this can be a problem if you are not careful. The automated tissue processors are extremely helpful in our lab because we process thousands of samples a year and the overall sample preservation is good.
David Cugier Associate Cellular and Molecular Biologist Abbott Labs 847-938-6725 David.Cugier-at-Abbott.com
kwalters {kwalters-at-blue.weeg. To: Microscopy-at-sparc5.microscopy.com uiowa.edu} cc: Subject: Lynx el Tissue Processor for EM samples 06/27/02 04:02 PM
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi,
Has anyone had experience with this automated processor of EM samples? Thanks for any information you can provide me.
I have been using the older Leica Lynx tissue processor (Now distributed by Electron Microscopy Sciences under the name Vision EMS processor) for 6 years and have been extremely pleased with the reliability, efficiency, and quality. There are a few potential short falls inherent with any automated tissue processor versus processing by hand which include the incorporation of air bubbles which can get trapped in the processing baskets and prevent good fluid exchange with the tissue. Also there is the potential problem of vials jamming if the vials are not seated securely in the carousel or if the vials are not symetrically round. I have not experienced jamming vials but I know this can be a problem if you are not careful. The automated tissue processors are extremely helpful in our lab because we process thousands of samples a year and the overall sample preservation is good.
David Cugier Associate Cellular and Molecular Biologist Abbott Labs 847-938-6725 David.Cugier-at-Abbott.com
kwalters {kwalters-at-blue.weeg. To: Microscopy-at-sparc5.microscopy.com uiowa.edu} cc: Subject: Lynx el Tissue Processor for EM samples 06/27/02 04:02 PM
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi,
Has anyone had experience with this automated processor of EM samples? Thanks for any information you can provide me.
I have been using the older Leica Lynx tissue processor (Now distributed by Electron Microscopy Sciences under the name Vision EMS processor) for 6 years and have been extremely pleased with the reliability, efficiency, and quality. There are a few potential short falls inherent with any automated tissue processor versus processing by hand which include the incorporation of air bubbles which can get trapped in the processing baskets and prevent good fluid exchange with the tissue. Also there is the potential problem of vials jamming if the vials are not seated securely in the carousel or if the vials are not symetrically round. I have not experienced jamming vials but I know this can be a problem if you are not careful. The automated tissue processors are extremely helpful in our lab because we process thousands of samples a year and the overall sample preservation is good.
David Cugier Associate Cellular and Molecular Biologist Abbott Labs 847-938-6725 David.Cugier-at-Abbott.com
kwalters {kwalters-at-blue.weeg. To: Microscopy-at-sparc5.microscopy.com uiowa.edu} cc: Subject: Lynx el Tissue Processor for EM samples 06/27/02 04:02 PM
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi,
Has anyone had experience with this automated processor of EM samples? Thanks for any information you can provide me.
} Morning Don, } } Please begin with: } } http://micro.magnet.fsu.edu/primer/techniques/polarized/polarizedhome.html } } Then, to get your colors from polarized light, you must understand } the phenomenon: } } http://micro.magnet.fsu.edu/primer/virtual/polarizing/ } } The key to having command of any tool is to understand how it works! The } microscope IS a tool! } } Regards, } } Fred Monson } } Frederick C. Monson, PhD } Center for Advanced Scientific Imaging } Schmucker II Science Center } West Chester University } South Church Street and Rosedale } West Chester, Pennsylvania, USA, 19383 } Phone: 610-738-0437 } FAX: 610-738-0437 } fmonson-at-wcupa.edu } CASI URL: http://darwin.wcupa.edu/casi/ } WCUPA URL: http://www.wcupa.edu/ } Visitors URL: http://www.wcupa.edu/_visitors/ } } } ---------- } From: danthony38-at-cogeco.ca } Sent: Friday, June 28, 2002 9:04 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Ask-A-Microscopist: Polarizer and Crystals in LM } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (danthony38-at-cogeco.ca) from } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday, } June 26, 2002 at 13:32:34 } -------------------------------------------------------------------------- } - } } Email: danthony38-at-cogeco.ca } Name: don anthony } } Education: 9-12th Grade High School } } Location: peterborough ont canada } } Question: I have seen beautiful photos of, (as I was told)chemicals } crystalized on glass slides. As it was explained to me, a polorizer } was placed in front of the light source,and a second polorizer, above } the crystals being photographed. The profusion of colour was } unbelieveable, and when the polorizer was moved slightly, the colours } changed, creating a different picture. My question is,after trying to } duplicate this proceedure, I'm getting no where, can anyone help? } Also what chemicals make for the best crystals. Many thanks Don } } -------------------------------------------------------------------------- } - } } }
We have a problem here, whereby the picture taken using the confocal microscopy is distorted in MetaMorph.The picture taken is 1mm by 1mm, but when the planes are rotated(90° angle), the x and y axis seem distored. I have been trying to calibrate the xy distances, but it doesn't seem to work. I was wondering if anyone can inform me the exact ways of calibrating the distances, or the reasons for the pictures being distorted.
Easiest way to do this is contacting UIC at http://www.universal-imaging.com and follow the links to support, they have on site scientific to help you out. "Abdul Rani, Suriani" {suriani-at-erc.montana.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi! } } We have a problem here, whereby the picture taken using the confocal } microscopy is distorted in MetaMorph.The picture taken is 1mm by 1mm, but } when the planes are rotated(90° angle), the x and y axis seem distored. I } have been trying to calibrate the xy distances, but it doesn't seem to work. } I was wondering if anyone can inform me the exact ways of calibrating the } distances, or the reasons for the pictures being distorted. } } Thank you for your help. } } Suriani Abdul Rani } Control Lab } CBE,MSU } Montana } } suriania-at-erc.montana.edu } }
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I would have to say that the blame is not so much on NT as it is on typical user practices. The security of any system relies a great deal on the security practices of its users.
That said - there are, not surprisingly, a number of solutions to the problem along the lines of what you suggest. Since system administration is a secondary part of many readers' job descriptions (myself included), I feel emphasis ought to be on easy to configure/implement. (I don't know any details on scripting a command to execute after a specified period without input; perhaps "at")
I should mention first that I don't use auditing or auto-logout, so I can't claim first-hand experience that might allow me to point out possible pitfalls. I do, however, always set the system to lock after a specified period (see below).
If the system is controlling instrumentation, or runs processes that take a long time (users likely to get coffee/lunch while waiting), I would suggest that the system be set to lock, rather than logoff. An auto-logoff will either hang waiting for applications to terminate, or terminate all applications if properly configured (losing unsaved data). This could be a real problem for some instruments, and upsetting for some users. If the system is set to lock, and a user forgets to logoff, anyone with administrative privileges (presumably you) can unlock the workstation in order to properly logoff the last user.
The easiest way I know to setup a local session timeout is to setup a password-protected screensaver. The first step here is to make sure users can't change the screensaver settings. This is best done using the NT system policy editor (NOT the Win 9x version). This is included with NT Server, and perhaps with the Resource Kit as well - I don't know of other ways to get it, but there may be. It can be installed on an NT workstation, using the NT Server disk.
If you have no way to obtain the policy editor, access to the screensaver tab (as well as just about anything else) can also be restricted by manually editing the registry. After restricting access to the screensaver settings, proceed as below:
To setup a lock timeout, just go to the screensaver tab, set the wait time reasonably short, and fill the "password protected" check-box -- doesn't get any easier!
To setup an auto-logoff timeout, you'll need to install and configure the winexit screensaver (included in the resource kit), as well as edit some permissions to keep it from hanging when trying to shut down programs.
My apologies to all if this has begun to wander a bit from the scope of this list, but many of us have to deal with these issues without proper background or support. (i.e. "winging it")
Ed: if you need further details I would suggest contacting me off the list.
Best,
Kevin Frischmann
Microscopy & Imaging Facility American Museum of Natural History Central Park West at 79th Street New York, NY 10024-5192 USA
To: Kevin Frischmann {kfrisch-at-amnh.org} Cc: Microscopy-at-sparc5.microscopy.com
Judy,
I believe you can obtain the information you need without installing any additional software. Windows NT has built-in tools for tracking users' logons/logoffs (among other things), though it's not enabled by default.
Click Start -} Programs -} Administrative Tools (Common) -} User Manager Select the Policies menu -} Audit..., and you'll find a window that allows you to enable auditing, and select which events are audited and logged. In your case, I'd recommend just enabling auditing for successful logons and logoffs. Everything selected will be logged to the Security Event Log: C:\WINNT\system32\config\SecEvent.Evt (Assuming %SystemRoot% is C:\WINNT\).
In order to view, filter, sort, and archive the log, as well as change settings like maximum file size and overwrite policy, use the Event Viewer: Click Start -} Programs -} Administrative Tools (Common) -} Event Viewer
Use Log -} Security to select the Security Log for viewing. I think you'll find the View -} Filter Events... selection will allow you to do everything you need, without having to import to a spreadsheet.
This is the easiest to configure option I can think of; If you need something a bit more sophisticated, and/or need to audit a large number of workstations, Fred Monson's suggestions are certainly more appropriate.
Best Regards,
Kevin Frischmann
Microscopy & Imaging Facility American Museum of Natural History Central Park West at 79th Street New York, NY 10024-5192 USA
At 05:06 PM 6/27/02 -0400, Judy Trogadis wrote: This topic may have been discussed, if so, I will look at the archives if you tell me an approximate date.
Some of the microscopes in our facility are sometimes left in a sorry state by inexperienced or hasty users. I would like to keep track of users who are actually capturing images, therefore using the computer (if fingerprint sensors exist, let me know, I can just dust the microscope knobs).
Has anyone used software that keeps a record of login ID's and hours of use? We are on NT operating systems.
Thank you
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 8Queen 30 Bond St. Toronto, ON M5B 1W8 Canada
The responses from the request for comments about the Lynx EM processor were extremely helpful, so I thought I'd go out on a limb and see if anyone had comments about the Leica EM processor.
I have collected the Lynx responses in a file and would be glad to send it on if anyone needs this information.
The responses from the request for comments about the Lynx EM processor were extremely helpful, so I thought I'd go out on a limb and see if anyone had comments about the Leica EM processor.
I have collected the Lynx responses in a file and would be glad to send it on if anyone needs this information.
i'm looking at some daguerreotypes (early photographs on Ag coated Cu plates) in the sem to find out about the silver tarnish that forms on the surfaces. as expected i find sulphur in it, but not in all the areas that look tarnished. i suspect that since the AgS layer may be thin when compared to the beam penetration, and that the underlying material is all Ag (with some Au toning agent) i may be swamping the signal from the tarnish layer. curiously the areas with the most intense interference colors are not the highest in S (no O present either). i've used low HV as well as high. since this is on museum materials i can't prep a cross section to see what's going on.
so the questions: will a tarnish layer on silver form a wedge of varying thickness around an aperture to the ambient air? and if a wedge forms will the interference colors indicate the local thicknesses accurately? does light play any role in tarnishing (some areas beneath a matte paper look untarnished, while adjacent areas outside the matte look heavily tarnished)? and as for the vanishing S signal...any ideas? is there a better way to probe the tarnish for thickness and composition (like SIMS)?
thx in advance. b-
---------------------------------------------- Brian McIntyre Electron Microscopy Lab- River Campus Univ of Rochester Rochester, NY 14627 716-275-3058/4875
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Listers, I am trying to find a source for heavy copper braid wire to repair some accessories used for metal and carbon evaporation. This is the stiff but flexible wire that would go between the connectors in a high vacuum evaporator and, in this case, the carbon evaporation apparatus. Does anyone have a source in the USA who is likely to sell small quantities? I just need a couple of feet at most.
Thanks, Debby
Debby Sherman Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building West Lafayette, IN 47907
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id JAA18989 for dist-Microscopy; Wed, 3 Jul 2002 09:19:56 -0500 (CDT) Received: from njz_spm_filter (sparc5 [206.69.208.10]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id JAA18984 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Wed, 3 Jul 2002 09:19:26 -0500 (CDT) Received: from mail.mcn.org (pop3.mcn.org [204.189.12.13]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with ESMTP id JAA18975 for {Microscopy-at-sparc5.Microscopy.Com} ; Wed, 3 Jul 2002 09:19:14 -0500 (CDT) Received: from [63.193.12.169] (ha-1o-men-p4-m13.mcn.org [63.193.12.169]) by mail.mcn.org (8.12.5/8.12.5) with ESMTP id g63EEOJK020723; Wed, 3 Jul 2002 07:14:28 -0700 (PDT) X-Sender: schooley-at-mail.mcn.org Message-Id: {v03007800b948b09743f4-at-[63.193.12.100]} Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
We are considering to purchase a Nikon SuperCoolscan 8000. Our TEM negative size is 8.0x9.9 cm and from reading of previous postings on this subject it appears that the film holder needs a bit of modification for TEM negatives to fit in without going through the hassle of trimming them (I am aware that the maximum area that I can scan is about 6.0x9.0, I just want to avoid trimming down hundreds of negatives).
I would really appreciate it if colleagues who have tried it (modifying the holder) successfully could please share their experience with me. I have only seen the 120/220 film holder and it did not seem to be very easy to modify.
Regards majid
..................................................... Majid Ghoddusi, DVM PhD Senior Microscopist Muscle Development Unit Children's Medical Research Institute locked Bag 23 Wentworthville NSW 2145 Australia http://www.cmri.com.au ........................................................
We are considering to purchase a Nikon SuperCoolscan 8000. Our TEM negative size is 8.0x9.9 cm and from reading of previous postings on this subject it appears that the film holder needs a bit of modification for TEM negatives to fit in without going through the hassle of trimming them (I am aware that the maximum area that I can scan is about 6.0x9.0, I just want to avoid trimming down hundreds of negatives).
I would really appreciate it if colleagues who have tried it (modifying the holder) successfully could please share their experience with me. I have only seen the 120/220 film holder and it did not seem to be very easy to modify.
Regards majid
.................................................... Majid Ghoddusi, DVM PhD Senior Microscopist Muscle Development Unit Children's Medical Research Institute locked Bag 23 Wentworthville NSW 2145 Australia http://www.cmri.com.au .......................................................
Try any electronics store, this kind of material is commonly used for attaching earth grounds, buss bars and the like. It also is used as a solder removal aid, or wick. (In the latter case, it is held against a connection that is to be de-soldered and heated with a soldering iron. The solder melts and wicks its way into the briad toward the heat, leaving the joint exposed.)
Too thin for your use? Braid three strands together, etc.
John Twilley
Debby Sherman wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Listers, } I am trying to find a source for heavy copper braid wire to repair some accessories used for metal and carbon evaporation. This is the stiff but flexible wire that would go between the connectors in a high vacuum evaporator and, in this case, the carbon evaporation apparatus. Does anyone have a source in the USA who is likely to sell small quantities? I just need a couple of feet at most. } } Thanks, } Debby } } } Debby Sherman Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } West Lafayette, IN 47907 } } } } }
At 08:45 AM 7/3/2002 -0500, Debby Sherman wrote: } I am trying to find a source for heavy copper braid wire to repair some accessories used for metal and carbon evaporation. This is the stiff but flexible wire that would go between the connectors in a high vacuum evaporator and, in this case, the carbon evaporation apparatus. Does anyone have a source in the USA who is likely to sell small quantities? I just need a couple of feet at most.
Unless you have special requirements for purity, just go to a well-equipped hardware store and ask for "copper grounding wire", a heavy gauge (6?) braid.
If you haven't already done so, I would suggest that you consult the people in the Image Permanence Institute of your own university. There are individuals there, or formerly associated with it, who have done considerable work in this area. Irving Pobboravsky comes to mind as one of the few contemporary practitioners who might be able to provide examples that could be sacrificed.
Just what the image forming structures are in a daguerreotype, and what interferes with their optical effects, continue to be a subject of research. The physical state of the surface has much to do with the scattering of light, and tarnish effects lead to short range redistribution of material in addition to the expected chemical reactions. One might expect to find some silver amalgam present as well.
SIMS certainly would seem to be a valuable option. The material would easily lend itself to ESCA or Auger techniques, as well.
John Twilley Conservation Scientist
Brian McIntyre wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi listers- } } i'm looking at some daguerreotypes (early photographs on Ag coated Cu } plates) in the sem to find out about the silver tarnish that forms on the } surfaces. as expected i find sulphur in it, but not in all the areas that } look tarnished. i suspect that since the AgS layer may be thin when } compared to the beam penetration, and that the underlying material is all } Ag (with some Au toning agent) i may be swamping the signal from the } tarnish layer. curiously the areas with the most intense interference } colors are not the highest in S (no O present either). i've used low HV as } well as high. since this is on museum materials i can't prep a cross } section to see what's going on. } } so the questions: will a tarnish layer on silver form a wedge of varying } thickness around an aperture to the ambient air? and if a wedge forms will } the interference colors indicate the local thicknesses accurately? does } light play any role in tarnishing (some areas beneath a matte paper look } untarnished, while adjacent areas outside the matte look heavily } tarnished)? and as for the vanishing S signal...any ideas? is there a } better way to probe the tarnish for thickness and composition (like SIMS)? } } thx in advance. } b- } } } ---------------------------------------------- } Brian McIntyre } Electron Microscopy Lab- River Campus } Univ of Rochester } Rochester, NY 14627 } 716-275-3058/4875 } } }
A significant component of this work could involve microscopy
Dear colleague,
A postdoctoral fellowship is available at the Centre for Visual Sciences, Australian National University. It would be appreciated if you could bring the attached advertisement to the attention of potential candidates.
yours sincerely
Gert Stange
ANUTECH Pty Ltd
and
AUSTRALIAN NATIONAL UNIVERSITY
Postdoctoral Fellow in Neurophysiology/Biorobotics
The Centre for Visual Sciences is seeking to fill a position to work on a challenging research project investigating the principles of visual flight control in insects. The research project, funded by a major aerospace organization, aims at identifying the spatial transfer characteristics of the optics and the spatiotemporal characteristics of the neuronal circuitry associated with the simple eyes (ocelli) of dragonflies. The successful applicant will apply optical, neuroanatomical, electrophysiological and behavioural techniques, with specific attention to object/motion detection mechanisms in the ocellar system and their integration with neuronal flight control circuitry. The biological results will be applied to the development of concepts for novel attitude control systems capable of being implemented in ultra-light hardware for application to micro-unmanned aerial vehicles.
Salary range will be $A 45,000 to $A 55,000. The position is for 1 year initially, with prospects for extension for a further 2 years subject to satisfactory performance and availability of project funding. Enquiries should be directed to Dr. Gert Stange, ph +61 2 6125 5089, email gert.stange-at-anu.edu.au. The selection criteria and duty statement can be obtained from or from Beverley Cooper, ph +61 2 6125 5865, email .
Applications close on Friday 17 August, 2002.
Applications, including the names and contact details of 3 referees, should be sent to Ms. Beverley Cooper, ANUTECH Pty Ltd., GPO Box 4, Canberra ACT 2601, fax +61 2 6257 1433.
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Thanks for all the replies regarding sources for copper braid. Most of the braid is tinned on the outside and can be either flat or round. A few of the responses are below.
1) If you disassemble co-axial cable you will find some copper braid wire within. Just remove this, attach crimp-on ends and you ae all set.
2) some local hardware stores corry it as it is used for grounding wire. It also is occasionally used to "wick" solder away from joins.
3) Newark Electronics sells what you want but you might have to buy 50-100 feet at about $30-$100. In Newark catalog 119, Beldon's braid is on page 1175 and Alpha's is on page 1239. Alpha calls theirs "tinned copper flat braid". My Beldon braid # 8660 carried 14.5 amps and had a cross section of about 3800 circular mils.
6) Ham radio folks often use braided wire as grounding wire.
7) This wire is often used in Physiology experiments where "noise" is often a problem. You might find it in a shop that repairs this equipment on your campus.
8) Try commercial dealers who sell High Vacuum equipment. They might be willing to sell some of the braid.
Debby
Debby Sherman Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building West Lafayette, IN 47907
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id JAA23882 for dist-Microscopy; Thu, 4 Jul 2002 09:57:11 -0500 (CDT) Received: from njz_spm_filter (sparc5 [206.69.208.10]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id JAA23879 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Thu, 4 Jul 2002 09:56:40 -0500 (CDT) Received: from crdsf1.arcride.edu.ar (crdsf1.arcride.edu.ar [200.9.237.3]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id JAA23872 for {Microscopy-at-sparc5.microscopy.com} ; Thu, 4 Jul 2002 09:56:28 -0500 (CDT) Received: from arcride.edu.ar by crdsf1.arcride.edu.ar id aa17645; 4 Jul 2002 11:54 EST Message-ID: {BasiliX-1.1.0-10257937973d245f05e7d34-at-wwwmail.ceride.gov.ar} X-Mailer: BasiliX 1.1.0 X-SenderIP: 200.9.237.5
Hello all, Please inform if theres someone who knows about the internal electronics of the detecting unit of a x-ray fluorescence which characteristics are:
EDAX PV9740/05
MODEL PV9500 165-17
ACTIVE AREA 10 MM2
AMPLIFIER MODEL 183 A
The problem is that some LED or PHOTODIODE of said detector burnt out. The detector is a vacuum packed unit and this diagnostic is the result of measurements on the detector connection pins guided by an electric circuit scheme.
If someone can give me advice, please contact me,
Tic. Ppal Elbio Martmnez CERIDE - CONICET G|emes 3450 3000 - Santa Fe Argentina email: edmarti-at-ceride.gov.ar
Hello all, Please inform if theres someone who knows about the internal electronics of the detecting unit of a x-ray fluorescence which characteristics are:
EDAX PV9740/05
MODEL PV9500 165-17
ACTIVE AREA 10 MM2
AMPLIFIER MODEL 183 A
The problem is that some LED or PHOTODIODE of said detector burnt out. The detector is a vacuum packed unit and this diagnostic is the result of measurements on the detector connection pins guided by an electric circuit scheme.
If someone can give me advice, please contact me,
Tic. Ppal Elbio Martmnez CERIDE - CONICET G|emes 3450 3000 - Santa Fe Argentina email: edmarti-at-ceride.gov.ar
Hello all, Please inform if there's someone who knows about the internal electronics of the detecting unit of a x-ray fluorescence which characteristics are:
EDAX PV9740/05
MODEL PV9500 165-17
ACTIVE AREA 10 MM2
AMPLIFIER MODEL 183 A
The problem is that some LED or PHOTODIODE of said detector burnt out. The detector is a vacuum packed unit and this diagnostic is the result of measurements on the detector connection pins guided by an electric circuit scheme.
If someone can give me advice, please contact me,
Tic. Ppal Elbio Martmnez CERIDE - CONICET G|emes 3450 3000 - Santa Fe Argentina email: edmarti-at-ceride.gov.ar
Hello All, I'm looking for the original article of Abrikosov( in russian or in english) in which he introduces the Type-II superconductor. I can't find it on the internet. Does anybody have the text or know where to look for it? Reference: A.A. Abrikosov, Zh. Eksperim. i Teor. Fiz. 32, 1442 (1957) [Sov. Phys. -- JETP 5, 1174 (1957)]
Thanks R. Almeida
************************************ Rogerio Lucio de Almeida
Does anyone have or know where I can buy a Leaf Lumina or MicroLumina camera. I understand the limitations of the technology, but for the price I am hard pressed to find a camera of equal resolution/dynamic range. Unless any one you have suggestions?
Thank you. Ken
-- Ken Tiekotter, Adjunct Professor The University of Portland Department of Biology 5000 N. Willamette Blvd. Portland, OR 97203
I guess you've already approached your friendly local Philips/EDAX agent?
I may be wrong, but I've always thought that the manufacturer is in a far better position to service these things than is anyone else.
Any interesting opinions out there?
cheers
rtch
} Date: Thu, 04 Jul 2002 11:43:17 ART } From: elbio martinez {edmarti-at-ceride.gov.ar} } Reply-to: edmarti-at-ceride.gov.ar } Subject: XRF Detector Unit PV9500 } To: Microscopy-at-sparc5.microscopy.com
} -------------------------------------------------------------------- } ---- The Microscopy ListServer -- Sponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------- } ---. } } } Hello all, } Please inform if theres someone who knows about the internal } electronics of the detecting unit of a x-ray fluorescence which } characteristics are: } } EDAX PV9740/05 } } MODEL PV9500 165-17 } } ACTIVE AREA 10 MM2 } } AMPLIFIER MODEL 183 A } } The problem is that some LED or PHOTODIODE of said detector burnt } out. The detector is a vacuum packed unit and this diagnostic is the } result of measurements on the detector connection pins guided by an } electric circuit scheme. } } If someone can give me advice, please contact me, } } } } Tic. Ppal Elbio Martmnez } CERIDE - CONICET } G|emes 3450 } 3000 - Santa Fe } Argentina } email: edmarti-at-ceride.gov.ar } Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
One would like to think so, but we're often disappointed in life.
I don't have the schematics on that particular unit, but the manufacturer would. Whether they would want to share that or not is another question. The item in question is probably used to reset the detector. Normally, the components within the vacuum would be only the detector diode and the FET used as a preamplifier, the remaining preamplifier parts are usually outside of the vacuum in a small metal box attached to the detector. However, even if they are inside the vacuum of the dewar cold finger assembly, that can be opened, repaired and re-pumped - if you have the right vacuum fitting. Again, the manufacturer could supply you with that, if they so desire.
The problem with manufacturers is often three fold - they often have difficulty holding on to good techs, their self-interest is often to sell you the latest system and they often see others working on their instruments (even needy customers) as cutting into their profits. A lot of oftens that occasionally add up to what is often seen as poor customer service.
Individuals will generally remain loyal to a manufacturer as long as they are able to meet that person's needs. If you've had a good result there, consider yourself lucky. At any particular time, there is at least one manufacturer that can actually provide good service with a view of their customer's needs. Unfortunately, none has ever kept that up for more than a few years. A customer's best friend is a good, local, service tech who stays with the manufacturer for a long time. Someone who has a talent for the work, good knowledge of the instruments, experience in working on them and the manufacturer's backing for parts and information, is a great asset.
I'm most aquainted with SEMS. Twenty years ago, ETEC, after being a market leader for many years, decided to get out of the business. Within the last couple of years, we've seen that again with Amray. Those are two extremes that used one business as a stepping stone to another. But in the ensuing years, every manufacturer out there has enjoyed periods of high sales, good customer relations, eroding sales, shifts in thoughts of service as a profit center rather than a vital part of design engineering and marketing leading to increases in turn-over rate and reduced knowledge and experience on the part of their service engineers.
In XRF, HNU comes to mind as an extreme of the first kind. Every other manufacturer as examples of the latter.
In many ways, it is a matter of the life cycle experienced by all businesses. This natural life cycle has recently become a factor in thought processes on business and the stock market. It is true across the board, no matter what sector a business is in. It is the reason every business has to be constantly re-inventing itself.
Now, Ritchie, to the heart of the matter, at least in a market the size of America. We have here technicians with decades of experience in such instrumentation. Many of them (I try to keep track of them as referrals) have gone into business for themselves. This is generally a desire to continue the work they love, without interference from corporations whose mergers, acquisitions and buy-outs continually dilute and obfuscate the customer's needs.
I am happy to here state that I am one of those who left that melee over twenty years ago. I have never really advertised my services, but have survived in business on word of mouth and benign exposure on the internet. In every case, my customers have sought me out. There is only one possible reason for that - the manufacturer has not provided the value that the customer expects. Frankly, there is no one more knowledgeable on what manufacturers are doing wrong, but not one has come to ask me what I would so willingly tell them.
I do make my share of mistakes, but I have more experience on more different systems from more manufacturers than just about anyone out there. But that breadth of experience, itself, brings real life practical knowledge that no manufacturer's tech can equal with his or hers limited experience and knowledge.
I've tooted my own horn enough, but actually, I've been tooting the horn of other independents out there. When you need us, it's good to know we're there. When you don't, you should be glad that there are others who do, because, in our own small way, we keep the manufacturer's feet on the ground. I could tell you some real horror stories of what manufacturers have pulled when they think that they have no competition, but I'll save that for another day.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174
On Friday, July 05, 2002 4:45 AM, Ritchie Sims [SMTP:r.sims-at-auckland.ac.nz] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } I guess you've already approached your friendly local Philips/EDAX } agent? } } I may be wrong, but I've always thought that the manufacturer is in a } far better position to service these things than is anyone else. } } Any interesting opinions out there? } } cheers } } rtch } } } } } } } } Date: Thu, 04 Jul 2002 11:43:17 ART } } From: elbio martinez {edmarti-at-ceride.gov.ar} } } Reply-to: edmarti-at-ceride.gov.ar } } Subject: XRF Detector Unit PV9500 } } To: Microscopy-at-sparc5.microscopy.com } } } -------------------------------------------------------------------- } } ---- The Microscopy ListServer -- Sponsor: The Microscopy Society } } of America To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------- } } ---. } } } } } } Hello all, } } Please inform if theres someone who knows about the internal } } electronics of the detecting unit of a x-ray fluorescence which } } characteristics are: } } } } EDAX PV9740/05 } } } } MODEL PV9500 165-17 } } } } ACTIVE AREA 10 MM2 } } } } AMPLIFIER MODEL 183 A } } } } The problem is that some LED or PHOTODIODE of said detector burnt } } out. The detector is a vacuum packed unit and this diagnostic is the } } result of measurements on the detector connection pins guided by an } } electric circuit scheme. } } } } If someone can give me advice, please contact me, } } } } } } } } Tic. Ppal Elbio Martmnez } } CERIDE - CONICET } } G|emes 3450 } } 3000 - Santa Fe } } Argentina } } email: edmarti-at-ceride.gov.ar } } } Ritchie Sims Phone : 64 9 3737599 ext 7713 } Department of Geology Fax : 64 9 3737435 } The University of Auckland email : r.sims-at-auckland.ac.nz } Private Bag 92019 } Auckland } New Zealand } } }
Does anyone of you have some experience you want to share about the Zeiss AxioCam HR? We are planning to buy one, but first I would like to know the positiv and negativ things about it. Thanks in advance,
On Fri, 5 Jul 2002 01:53:31 -0700 Allen Sampson {ars-at-sem.com} wrote: } The problem with manufacturers is often three fold - they often have } difficulty holding on to good techs, their self-interest is often to sell } you the latest system and they often see others working on their } instruments (even needy customers) as cutting into their profits. A lot of } oftens that occasionally add up to what is often seen as poor customer } service. } } Individuals will generally remain loyal to a manufacturer as long as they } are able to meet that person's needs. If you've had a good result there, } consider yourself lucky. At any particular time, there is at least one } manufacturer that can actually provide good service with a view of their } customer's needs. Unfortunately, none has ever kept that up for more than } a few years.
I wish to report that in the 25 years I have worked here we have had continuous good service from a manufacturer.
During that time there have been a series of local engineers most of whom have been replaced as they progressed within the company. I do not feel that we have ever been penalised for carrying out our own service, indeed they have been helpful in supplying components, information and even redundant items from scrapped instruments to keep older instruments running. We have also told them of alternative sources that we have found for their repairs or components.
I accept that the situation may be different in other parts of the world and that the original comments probably refer to the USA but I wish to correct the impression that manufacturers cannot keep up a high standard of service for more than a few years. It is possible.
With any complex piece of equipment there is the possiblilty of problems, the most important thing is for the customer, the supplier and the service organisation to have trust and faith in each other.
It takes hard work from all sides to build up this sort of relationship, but it is worth it.
Ron Disclaimer: I probably buy the engineers as many beers as they buy me so I don't feel under any obligation.
---------------------- Mr. R.C. Doole Department of Materials, University of Oxford. Parks Road, Oxford. OX1 3PH. UK. Phone +44 (0) 1865 273701 Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
Perhaps something about wanting to remain on good terms with a customer with the visibility of Oxford University has something to do with it? A firm named "Widget Tool Alloys" might be perceived somewhat differently by a manufacturer.
Certainly in the U.S. the attitude of many OEMs often becomes "That's a really old model... I'm sure we don't have any documentation on that. Most of our customers have migrated to the _____ platform". While that is probably true in the case of rapidly changing data handling systems, it is less true of other systems. In my experience this has been particularly galling when it comes to mechanical systems (for which nothing short of abuse should cause them to wear out) and for detector electronics where the the nature of the detection process and the signal conditioning requirements remained static for about two decades.
Disclaimer: If Widget Tool Alloys actually exists out there somewhere, my apologies. No disrespect is intended.
John Twilley
Ron Doole wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } On Fri, 5 Jul 2002 01:53:31 -0700 Allen Sampson } {ars-at-sem.com} wrote: } } The problem with manufacturers is often three fold - they often have } } difficulty holding on to good techs, their self-interest is often to sell } } you the latest system and they often see others working on their } } instruments (even needy customers) as cutting into their profits. A lot of } } oftens that occasionally add up to what is often seen as poor customer } } service. } } } } Individuals will generally remain loyal to a manufacturer as long as they } } are able to meet that person's needs. If you've had a good result there, } } consider yourself lucky. At any particular time, there is at least one } } manufacturer that can actually provide good service with a view of their } } customer's needs. Unfortunately, none has ever kept that up for more than } } a few years. } } I wish to report that in the 25 years I have worked here we } have had continuous good service from a manufacturer. } } During that time there have been a series of local } engineers most of whom have been replaced as they } progressed within the company. I do not feel that we have } ever been penalised for carrying out our own service, } indeed they have been helpful in supplying components, } information and even redundant items from scrapped } instruments to keep older instruments running. We have also } told them of alternative sources that we have found for } their repairs or components. } } I accept that the situation may be different in other } parts of the world and that the original comments probably } refer to the USA but I wish to correct the impression that } manufacturers cannot keep up a high standard of service for } more than a few years. It is possible. } } With any complex piece of equipment there is the } possiblilty of problems, the most important thing is for } the customer, the supplier and the service organisation to } have trust and faith in each other. } } It takes hard work from all sides to build up this sort of } relationship, but it is worth it. } } Ron } Disclaimer: I probably buy the engineers as many beers as } they buy me so I don't feel under any obligation. } } ---------------------- } Mr. R.C. Doole } Department of Materials, } University of Oxford. } Parks Road, Oxford. OX1 3PH. UK. } Phone +44 (0) 1865 273701 } Fax +44 (0) 1865 283333 } ron.doole-at-materials.ox.ac.uk
Someone has asked me to do some work for them on mineral specimens. The samples will be mounted in resin and polished and we will then coat with carbon. Obviously we will set our software to deconvolute carbon from the analyses. We have the option to coat samples with either gold or silver and then look at the carbon content. I suppose the questions I would like to have answered are:
(1) Heavy elements like gold or silver will absorb some of the light element (inc. carbon) X-rays when used as coatings. Is there any way of correcting for this to get an accurate quantitative analysis of carbon content?
(2) Is there any way of removing either gold/silver or carbon coatings from such samples except for the obvious method of regrinding/polishing the coating off?
Thanks in advance.
Regards,
Chris Peppiatt
============================================ Dr. Chris Peppiatt (Experimental Officer), The National Centre for Biomedical Engineering Science, Science & Engineering Technology Building, National University of Ireland Galway, Galway City, Co. Galway, Republic of Ireland. chris.peppiatt-at-nuigalway.ie Phone: +353 91 512157 Fax: +353 91 750596 =============================================
Someone has asked me to do some work for them on mineral specimens. The samples will be mounted in resin and polished and we will then coat with carbon. Obviously we will set our software to deconvolute carbon from the analyses. We have the option to coat samples with either gold or silver and then look at the carbon content. I suppose the questions I would like to have answered are:
(1) Heavy elements like gold or silver will absorb some of the light element (inc. carbon) X-rays when used as coatings. Is there any way of correcting for this to get an accurate quantitative analysis of carbon content?
(2) Is there any way of removing either gold/silver or carbon coatings from such samples except for the obvious method of regrinding/polishing the coating off?
Thanks in advance.
Regards,
Chris Peppiatt
============================================ Dr. Chris Peppiatt (Experimental Officer), The National Centre for Biomedical Engineering Science, Science & Engineering Technology Building, National University of Ireland Galway, Galway City, Co. Galway, Republic of Ireland. chris.peppiatt-at-nuigalway.ie Phone: +353 91 512157 Fax: +353 91 750596 =============================================
} } Does anyone have or know where I can buy a Leaf Lumina or MicroLumina } camera. I understand the limitations of the technology, but for the price } I am hard pressed to find a camera of equal resolution/dynamic range. } Unless any one you have suggestions? } } Thank you. } Ken } } -- Ken, Check Electron Microscopy Sciences. No financial interest, just a long-time customer. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Morning Debby, When I had this problem, I went to Pep Boys [no stock or other relationship] and purchased some stranded copper battery cable. I had to 'trim' off the connectors, but the solution was satisfactory. Another choice is any electric supply store that sells to contractors or a 'Home Depot' [no stock or other relationship], though may be more likely to find stranded Al than Cu there. I have always found stranded Cu at one or the other of these places. Contractor supplies and Home Depot are usually less expensive, because the cable is sold by the foot. Such cable is used in many high wattage situations.
Hope this helps,
Fred Monson
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center West Chester University South Church Street and Rosedale West Chester, Pennsylvania, USA, 19383 Phone: 610-738-0437 FAX: 610-738-0437 fmonson-at-wcupa.edu CASI URL: http://darwin.wcupa.edu/casi/ WCUPA URL: http://www.wcupa.edu/ Visitors URL: http://www.wcupa.edu/_visitors/
} ---------- } From: Debby Sherman } Reply To: Debby Sherman } Sent: Wednesday, July 3, 2002 9:45 AM } To: message to: MSA list } Subject: copper braid } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Listers, } I am trying to find a source for heavy copper braid wire to repair some } accessories used for metal and carbon evaporation. This is the stiff but } flexible wire that would go between the connectors in a high vacuum } evaporator and, in this case, the carbon evaporation apparatus. Does } anyone have a source in the USA who is likely to sell small quantities? I } just need a couple of feet at most. } } Thanks, } Debby } } } Debby Sherman Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } } Purdue University E-mail: dsherman-at-purdue.edu } } S-052 Whistler Building } West Lafayette, IN 47907 } } } }
I would be quite cautious in trying to directly measure carbon. We have wanted to for years, but I tell clients not to expect much.
We can easily see carbon in carbonate minerals. I just do not try to quantitate it directly. (The times I tried, I got very doubtful numbers. I would like my results to be consistent within 50% relative error.) If you are looking for carbon in lesser amounts, I am doubtful that it would work.
You are aware that the carbon is strongly absorbed by most other elements. I understand that it is a big part of the problem. Even if you can get a good carbon signal above background, you still have to deal with uncertainty in the absorption coefficients.
There would also be problems if any of your resin smears over.
Maybe you can tell us a bit more what you would like to measure and we can provide more information.
I recall a discussion a couple of months back about removing Au and Ag by means other that polishing. It should be in the list archives.
Warren
At 11:57 AM 7/8/02 +0100, you wrote: } Dear All, } } Someone has asked me to do some work for them on mineral specimens. The } samples will be mounted in resin and polished and we will then coat with } carbon. Obviously we will set our software to deconvolute carbon from the } analyses. We have the option to coat samples with either gold or silver } and then look at the carbon content. I suppose the questions I would like } to have answered are: } } (1) Heavy elements like gold or silver will absorb some of the light } element (inc. carbon) X-rays when used as coatings. Is there any way of } correcting for this to get an accurate quantitative analysis of carbon content? } } (2) Is there any way of removing either gold/silver or carbon coatings } from such samples except for the obvious method of regrinding/polishing } the coating off? } } Thanks in advance. } } Regards, } } Chris Peppiatt
------------------------------------------- No files should be attached to this message ------------------------------------------- Warren E. Straszheim, Ph.D. Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
Listers: If any of you have experience with a major retrofit to an ElectroScan E-3 ESEM for fully automated control (stage, gas pressure, imaging, EDS, etc.), I would like to have your thoughts - both good news and bad. Thanks, Bill
William A. Heeschen, Ph.D. Microscopy, Digital Imaging The Dow Chemical Company Midland, Mi 48667 waheeschen-at-dow.com
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Upon some serious cleaning we have discovered three LKB glass knife makers that we do not need to keep. We have one Model 7800 and two Model 7801 Type As that are in good working order. If you are interested please reply to me off line with an offer. Tom
Thomas Moninger (thomas-moninger-at-uiowa.edu) University of Iowa Central Microscopy Research Facility (www.uiowa.edu/~cemrf) View expressed are my own.
Upon some serious cleaning we have discovered three LKB glass knife makers that we do not need to keep. We have one Model 7800 and two Model 7801 Type As that are in good working order. If you are interested please reply to me off-line with an offer. Tom
Thomas Moninger (thomas-moninger-at-uiowa.edu) University of Iowa Central Microscopy Research Facility (www.uiowa.edu/~cemrf) View expressed are my own.
I am forwarding this as information about a volume that serves as a 'limited access' ramp to the pre-1950's protocols and literature on staining and microtechnique. The price I have been given for one is $84.50+. The other relevant information is below in the remainder of the thread.
The last of Peter Gray is on the horizon. He has lasted longer than most. This book has been discussed on the Histopathology Net List recently. You can search at: http://www.histosearch.com/histonet.html
I have no business relationship with Krieger.
Regards,
Fred Monson
} ---------- } From: Krieger } Sent: Monday, July 8, 2002 6:06 PM } To: Monson, Frederick C. } Subject: Re: Gray, Microtomist's Formulary and Guide } } Dear Dr. Monson: } We are selling the remaining stock we have. At the present time we have } approximately 113 copies available. If you are interested in purchasing } large quantities (over 50 copies) we can offer you special discounts. } Sincerely, } Cheryl Stanton } Krieger Publishing Company } P.O. Box 9542 } Melbourne, FL 32902-9542 } Tel: (321) 724-9542 } Fax: (321-951-3671 } 1-800-724-0025 } E-mail: info-at-krieger-publishing } www.krieger-publishing.com } } -----Original Message----- } From: Monson, Frederick C. {fmonson-at-wcupa.edu} } To: 'Krieger' {info-at-krieger-publishing.com} } Date: July 08, 2002 1:52 PM } Subject: RE: Gray, Microtomist's Formulary and Guide } } } } Hi Cheryl, } } I know that you are modifying your web site, but when I called up } } your list of books, this one was NOT included. If I am to recommend the } } book, I must know its current disposition. For example, do you consider } it } } a current, and future, publication? Are you simply selling off the last } of } } the last run? } } } } Thanks, } } } } Fred Monson } } } } } ---------- } } } From: Krieger } } } Sent: Monday, July 8, 2002 11:47 AM } } } To: Monson, Frederick C. } } } Subject: Re: Gray, Microtomist's Formulary and Guide } } } } } } Dear Dr. Monson: } } } } } } Please be advised this book is available at a list price of $84.50 } plus } } } $5.00 for shipping via Ground UPS. Please advise if you require } further } } } information. } } } } } } Sincerely, } } } Cheryl Stanton } } } Krieger Publishing Company } } } P.O. Box 9542 } } } Melbourne, FL 32902-9542 } } } Tel: (321) 724-9542 } } } Fax: (321-951-3671 } } } 1-800-724-0025 } } } E-mail: info-at-krieger-publishing } } } www.krieger-publishing.com } } } } } } -----Original Message----- } } } From: Monson, Frederick C. {fmonson-at-wcupa.edu} } } } To: 'info-at-krieger-publishing.com' {info-at-krieger-publishing.com} } } } Date: July 08, 2002 11:35 AM } } } Subject: Gray, Microtomist's Formulary and Guide } } } } } } } } } } Is this one now out of print for good? } } } } } } } } Thanks for help, } } } } } } } } Frederick C. Monson, PhD } } } } Center for Advanced Scientific Imaging } } } } Schmucker II Science Center } } } } West Chester University } } } } South Church Street and Rosedale } } } } West Chester, Pennsylvania, USA, 19383 } } } } Phone: 610-738-0437 } } } } FAX: 610-738-0437 } } } } fmonson-at-wcupa.edu } } } } CASI URL: http://darwin.wcupa.edu/casi/ } } } } WCUPA URL: http://www.wcupa.edu/ } } } } Visitors URL: http://www.wcupa.edu/_visitors/ } } } } } } } }
Dear All, Here is the problem we had. we are trying to localize the metals(Zn and Cd) within the planttissues (Barley). Prior work has shown that if you fix the tissue in FAA or gluteraldehyde-type fixes and continue with dehydration steps, the metals leach or diffuse out of the plant into the fix or dehydrating agent.Metal localization has mostly been done using cryofixation and with a cryostage. As the cryostage is not avilable at our EM Center. We fixed the samples conventionally (gluteraldehyde, ethanol dehydration)and cryofractured in liquid N and subjected them to EDS system, but it could barely pick up the metal in the tissue. but the analytical studies (ICP) showed theres enough metal accumulation. some of the alternatives we are thinking is- Treating the tissue samples with chemicals to precipitate the metals and continue with conventional procedures with minimum dehydration and fixation steps. Do any of you know of stains for metals (Zn& Cd) that would allow us to stain the cryosections specifically for metals and view them by LM? Or do you have any better or alternative suggestions? We would appreciate any input and suggestions you may have. thanks in advance B.B.M.Sridhar Graduate Research Assistant Diagnostic Instrumentation and Analysis Laboratory (DIAL) & Department of Forest Products Mississippi State University Starkville, MS -39759 Phone(office)- 662-325-9044
Dear All, Here is the problem we had. we are trying to localize the metals(Zn and Cd) within the planttissues (Barley). Prior work has shown that if you fix the tissue in FAA or gluteraldehyde-type fixes and continue with dehydration steps, the metals leach or diffuse out of the plant into the fix or dehydrating agent.Metal localization has mostly been done using cryofixation and with a cryostage. As the cryostage is not avilable at our EM Center. We fixed the samples conventionally (gluteraldehyde, ethanol dehydration)and cryofractured in liquid N and subjected them to EDS system, but it could barely pick up the metal in the tissue. but the analytical studies (ICP) showed theres enough metal accumulation. some of the alternatives we are thinking is- Treating the tissue samples with chemicals to precipitate the metals and continue with conventional procedures with minimum dehydration and fixation steps. Do any of you know of stains for metals (Zn& Cd) that would allow us to stain the cryosections specifically for metals and view them by LM? Or do you have any better or alternative suggestions? We would appreciate any input and suggestions you may have. thanks in advance B.B.M.Sridhar Graduate Research Assistant Diagnostic Instrumentation and Analysis Laboratory (DIAL) & Department of Forest Products Mississippi State University Starkville, MS -39759 Phone(office)- 662-325-9044
I recall that over the past couple of years several people have inquired about ways to control the growth of algae in recirculating cooling water systems.
I just received a catalog from an outfit called Home Improvements that lists a device called 'Power Clear' for controlling algal growth in garden pools and ponds. It is essentially a 15 x 5 x 5" chamber with hose connections at each end. When the circulator's hose is connected to it the circulating water flows over a quartz-sleeved ultra violet bulb, which is said to kill parasites, mold spores, bacteria and fungi in the water.
It sounds as though this would be very suitable to use in water recirculators for electron microscopes and related instruments. The cost is only about $130, with replacement bulbs priced at $30. Check www.ImprovementsCatalog.com, or call 1-800-642-2112.
-- Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan 3062 Dow Bldg.; 2300 Hayward St. Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (brandon.k.rice-at-uwrf.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, July 8, 2002 at 11:20:50 ---------------------------------------------------------------------------
Question: I am using a .50 NA objective and three lenses to image 1.6 micron silica spheres onto a camera. The focal lengths of the three lenses after the objective are 125mm, 38.1mm, and 90mm respectively. I am utilizing Kohler illumination to illuminate the sample. The image of the spheres appear very stretched out in the vertical direction; the spheres look more like ellipses. I have attempted to realign the objective and lenses over and over, but this does not seem to affect the image. What do you recommend I try to make the spheres look spherical?
My colleague asks: Is it true or is a hoax that there is a software which makes it possible to get focussed light microscopy images from pairs of under- and overfocussed micrographs? If it si true, what is the name of the software and where is it available? Thank you. M. Kalab
on 7/9/02 11:34 AM, Maruthi Sridhar Balaji Bhaskar at sb183-at-Ra.MsState.Edu wrote:
} Here is the problem we had. we are trying to localize the metals(Zn } and Cd) within the planttissues (Barley). Prior work has shown that if you } fix } the tissue in FAA or gluteraldehyde-type fixes and continue with dehydration } steps, the metals leach or diffuse out of the plant into the fix or } dehydrating } agent.Metal localization has mostly been done using cryofixation and with a } cryostage. As the cryostage is not avilable at our EM Center. We fixed the } samples conventionally (gluteraldehyde, ethanol dehydration)and cryofractured } in liquid N and subjected them to EDS system, but it could barely pick up the } metal in the tissue. but the analytical studies (ICP) showed theres enough } metal accumulation. } some of the alternatives we are thinking is- } Treating the tissue samples with chemicals to precipitate the metals and } continue with conventional procedures with minimum dehydration and fixation } steps. } Do any of you know of stains for metals (Zn& Cd) that would allow us to stain } the cryosections specifically for metals and view them by LM? Or do you have } any better or alternative suggestions? } We would appreciate any input and suggestions you may have. } thanks in advance Dear Maruthi, Can you cryo-fix, then lyophylize at low temperatures (-90 C)? This should retain the metals, but much of the structure will be hard to visualize. If you can recognize the structures where the metals appear, you can get a rough idea of the location of the metals, then try other techniques to pin down the metals to better resolution if needed. Good luck. Yours, Bill Tivol
Has anybody out there experience with using chicken ab´s as primary ab´s for immunogold labeling? Do they have disatvantages compared with rabbit, mouse ab´s. I can remember that a couple of years ago there was the word that the available anti-chicken-gold-conjugates are not as "good" as anti-rabbit- or mouse-gold-conjugates.
Thanks for your help,
Stefan
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° Stefan Geimer MCDB Dept. Yale University P.O. Box 208103 New Haven, CT 06520-8103 U.S.A.
The recirculating cooling water systems problem that we have the most problem trying to get a handle on is controlling the pH of the cooling water to prevent corrosion. Since the end of the good old days when we were allowed to use Sodium Chromate and Sodium Bicarbonate we have not yet found a satisfactory replacement.
Satisfactory includes; effectiveness at pH control, life time before depletion, safe handling and disposal, cost, availablility.
Trying to get good advice also seems to like the proverbial hens teeth.
Anyone out there come up with any good products recently.
Allan
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-- ------------------------------------------------- Allan Mitchell Technical Manager Otago Centre for Electron Microscopy C/-Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
The recirculating cooling water systems problem that we have the most problem trying to get a handle on is controlling the pH of the cooling water to prevent corrosion. Since the end of the good old days when we were allowed to use Sodium Chromate and Sodium Bicarbonate we have not yet found a satisfactory replacement.
Satisfactory includes; effectiveness at pH control, life time before depletion, safe handling and disposal, cost, availablility.
Trying to get good advice also seems to like the proverbial hens teeth.
Anyone out there come up with any good products recently.
Allan
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-- ------------------------------------------------- Allan Mitchell Technical Manager Otago Centre for Electron Microscopy C/-Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Has anybody out there experience with using chicken ab´s as primary ab´s
for immunogold labeling? Do they have disatvantages compared with rabbit, mouse ab´s. I can remember that a couple of years ago there was the word that the available anti-chicken-gold-conjugates are not as "good" as anti-rabbit- or anti-mouse-gold-conjugates.
Thanks for your help,
Stefan
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° Stefan Geimer c/o Rosenbaum Lab. MCDB Dept. Yale University P.O. Box 208103 New Haven, CT 06520-8103 U.S.A.
I have the following problem: I am observing Epon sections, stained with uranyl acetate and lead citrate in an Philips EM 201. The sections show a really severe contamination with electron dense grains. These grains are always associated with embedded structures (membranes, microtubules etc.).The problem started after I changed the cathode. The contamination looks kinda like lead-stain granularity and I think it has something to do with the lead. A section stained with uranyl acetate only looks fine (but I need the lead to get enough stain). The crazy thing is that I don´t have the problem when I use our other scope, a Zeiss EM 10. So if I take one of my sections and have a look at it in the Zeiss EM 10, everything is perfect. The same section observed in the Philips EM 201 shows this contamination with electron dense grains. Both scopes operated at comparable conditions (80 kv, cold trap etc.). So the contamination seems to be lead citrate and scope dependend. Anybody ever had that problem and might have an idea how to solve it?
Stefan
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° Stefan Geimer MCDB Dept. Yale University P.O. Box 208103 New Haven, CT 06520-8103 U.S.A.
Does anyone know of US sources for the Sony UD-890 and UD-895CE video printers? I'd like to see if they would interface to NTSC 640x480 composite video.
} Date: Tue, 09 Jul 2002 16:12:38 -0400 } From: Wil Bigelow {bigelow-at-engin.umich.edu} } Subject: Stop algae growth } X-Sender: bigelow-at-srvr5.engin.umich.edu } To: Microscopy Listserver {microscopy-at-sparc5.microscopy.com} } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-- Dr. Lawrence F. Allard Distinguished Research Staff Member High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 PO Box 2008 Oak Ridge, TN 37831-6064
I've noticed a rash of double posting lately. If you are getting an error message on your posting please contact me. Do not simply try posting a second time.
Nestor Your Friendly Neighborhood SysOp -- Nestor J. Zaluzec Argonne National Lab Materials Science Division.
We looked into the use of UV sterilization (both those that generate ozone and those that do not) for purposes of reducing or eliminating algae growth in fountains containing artworks that are susceptible to staining or corrosion by some of the chemical agents typically used for the same purpose. I've also had to deal with the problem in closed recirculating cooling systems. The problem is that the effects of UV are limited to the flow-through cell and algae can still colonize the walls of the rest of the system. When bits of the biofilm come off, they can block filter screens and or the aperture that typically lies behind the anode of a fine-focus X-ray tube. It doesn't matter that the debris got sterilized on its trip through the UV lamp housing.
My experience with closed systems has been best when using only reverse osmosis purified water with a very small amount of biocide. If there is no source of minerals, the growth is retarded and cleaning may be limited to three or four year intervals. Often, the nylon mesh of the intake filter was the limiting factor, beginning to embrittle and give way, thereby introducing its own problems.
John Twilley
Wil Bigelow wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I recall that over the past couple of years several people have } inquired about ways to control the growth of algae in recirculating } cooling water systems. } } I just received a catalog from an outfit called Home Improvements } that lists a device called 'Power Clear' for controlling algal growth } in garden pools and ponds. It is essentially a 15 x 5 x 5" chamber } with hose connections at each end. When the circulator's hose is } connected to it the circulating water flows over a quartz-sleeved } ultra violet bulb, which is said to kill parasites, mold spores, } bacteria and fungi in the water. } } It sounds as though this would be very suitable to use in water } recirculators for electron microscopes and related instruments. The } cost is only about $130, with replacement bulbs priced at $30. Check } www.ImprovementsCatalog.com, or call 1-800-642-2112. } } -- } Wilbur C. Bigelow, Prof. Emeritus } Materials Sci. & Engr., University of Michigan } 3062 Dow Bldg.; 2300 Hayward St. } Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; } Fx:734-763-4788; Ph:734-662-5237
Quantitative analysis of Carbon by EDX is nearly impossible because of the very shallow penetration depth of the Carbon X-rays. You really just measure the surface. Surface analysis techniques such as XPS and Auger also show that thin carbon films love to form on surfaces. If you have an XPS you use your ion gun to sputter off the carbon surface scum to see the real surface.
Dry ashing in a plasma cleaner can also remove the carbon surface layer. Sputter etching can be used to remove gold and silver coatings.
Ron Vane XEI Scientific 3124 Wessex Way, Redwood City, CA 94061 650-369-0133 www.SEMCLEAN.com
Note: XEI Scientific makes the EVACTRON plasma cleaning system for Electron Microscope Chambers and FIBs, but does not make desk top plasma dry ashers or sputter etchers.
-----Original Message----- } From: Chris Peppiatt {chris.peppiatt-at-nuigalway.ie} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
In the same idee, what about mesurements on boron carbide ? Carbon AND boron concentrations ! I see nice spectras, without anything else ( a bit O, some times Al and N). And I have variations between samples in the B/C ratio. In such a case can I mesure only ratio variation, or is it possible to try some quantification (with standards). The sample is bulk B4C and laser ablation thick layers (with dropplets). Primary energy 3 to 5 keV.
I think it's a bit pretentious to quantify. What is other's opinion ?
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess 67037 Strasbourg CEDEX France
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Chris and all: } } Quantitative analysis of Carbon by EDX is nearly impossible because of the } very shallow penetration depth of the Carbon X-rays. You really just measure } the surface. Surface analysis techniques such as XPS and Auger also show } that thin carbon films love to form on surfaces. If you have an XPS you use } your ion gun to sputter off the carbon surface scum to see the real surface. } } Dry ashing in a plasma cleaner can also remove the carbon surface layer. } Sputter etching can be used to remove gold and silver coatings. } } Ron Vane } XEI Scientific } 3124 Wessex Way, Redwood City, CA 94061 } 650-369-0133 } www.SEMCLEAN.com } } Note: XEI Scientific makes the EVACTRON plasma cleaning system for Electron } Microscope Chambers and FIBs, but does not make desk top plasma dry ashers } or sputter etchers. } } -----Original Message----- } } From: Chris Peppiatt {chris.peppiatt-at-nuigalway.ie} } To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com} } Date: Monday, July 08, 2002 3:22 PM } Subject: Carbon Quantitation by EDX } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Dear All, } } } } Someone has asked me to do some work for them on mineral specimens. The } } samples will be mounted in resin and polished and we will then coat with } } carbon. Obviously we will set our software to deconvolute carbon from the } } analyses. We have the option to coat samples with either gold or silver and } } then look at the carbon content. I suppose the questions I would like to } } have answered are: } } } } (1) Heavy elements like gold or silver will absorb some of the light } } element (inc. carbon) X-rays when used as coatings. Is there any way of } } correcting for this to get an accurate quantitative analysis of carbon } content? } } } } (2) Is there any way of removing either gold/silver or carbon coatings from } } such samples except for the obvious method of regrinding/polishing the } } coating off? } } } } Thanks in advance. } } } } Regards, } } } } Chris Peppiatt } } } } } } ============================================ } } Dr. Chris Peppiatt (Experimental Officer), } } The National Centre for Biomedical Engineering Science, } } Science & Engineering Technology Building, } } National University of Ireland Galway, } } Galway City, Co. Galway, } } Republic of Ireland. } } chris.peppiatt-at-nuigalway.ie } } Phone: +353 91 512157 Fax: +353 91 750596 } } ============================================= } } } } } } } }
When you take a "contaminated" section from the Philips does it then look OK in the Zeiss?
Dave
On Tue, 09 Jul 2002 20:04:58 +0200 Stefan Geimer {stefan.geimer-at-yale.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have the following problem: } I am observing Epon sections, stained with uranyl acetate and lead } citrate in an Philips EM 201. The sections show a really severe } contamination with electron dense grains. These grains are always } associated with embedded structures (membranes, microtubules etc.).The } problem started after I changed the cathode. The contamination looks } kinda like lead-stain granularity and I think it has something to do } with the lead. A section stained with uranyl acetate only looks fine } (but I need the lead to get enough stain). } The crazy thing is that I don´t have the problem when I use our other } scope, a Zeiss EM 10. So if I take one of my sections and have a look at } it in the Zeiss EM 10, everything is perfect. The same section observed } in the Philips EM 201 shows this contamination with electron dense } grains. Both scopes operated at comparable conditions (80 kv, cold trap } etc.). So the contamination seems to be lead citrate and scope } dependend. } Anybody ever had that problem and might have an idea how to solve it? } } Stefan } } } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } Stefan Geimer } MCDB Dept. } Yale University } P.O. Box 208103 } New Haven, CT 06520-8103 } U.S.A. } } Tel.: 203/432-3473 } Fax.: 203/432-6210 } } e-mail: stefan.geimer-at-yale.edu } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Dobry den, Milosi, metoda se jmenuje "deconvolution" a je k mani v cele rade image analysis softwares. Napr. Northern Eclipse od Empix Imaging a mnohe dalsi. Jak je v Ottawe? Jeste pisete pro Neviditelneho Psa? Uz ho nectu, protoze na to naveseli tolik reklam, ze to muj staricky pocitac nezvlada. Mnoho pozdravu, Sarka Lhotakova
On Tue, 9 Jul 2002, Ann-Fook Yang wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } My colleague asks: } Is it true or is a hoax that there is a software which makes it } possible to get focussed light microscopy images from pairs of under- } and overfocussed micrographs? If it si true, what is the name of the } software and where is it available? } Thank you. } M. Kalab } } Please reply to: } scimat-at-magma.ca } }
Wil, I would think this would kill the algae in the water and the UV chamber but other areas are still vulnerable. We made the mistake of installing a cooling water tap off our Haskris system on an EM400 for digital camera cooling by using clear tubing. The algae growth took off. I contacted Philips to determine how we could treat the coolant to prevent this algae growth. They recommended a 50 / 50, water / ethylene glycol. We did this and had no further problems with algae even with the clear tubing in place. I would not do this without the OK from you scope vendor. Russ Gillmeister Xerox ~~~~~~~~~~~~~
-----Original Message----- } From: Wil Bigelow [mailto:bigelow-at-engin.umich.edu] Sent: Tuesday, July 09, 2002 4:13 PM To: Microscopy Listserver
I recall that over the past couple of years several people have inquired about ways to control the growth of algae in recirculating cooling water systems.
I just received a catalog from an outfit called Home Improvements that lists a device called 'Power Clear' for controlling algal growth in garden pools and ponds. It is essentially a 15 x 5 x 5" chamber with hose connections at each end. When the circulator's hose is connected to it the circulating water flows over a quartz-sleeved ultra violet bulb, which is said to kill parasites, mold spores, bacteria and fungi in the water.
It sounds as though this would be very suitable to use in water recirculators for electron microscopes and related instruments. The cost is only about $130, with replacement bulbs priced at $30. Check www.ImprovementsCatalog.com, or call 1-800-642-2112.
-- Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan 3062 Dow Bldg.; 2300 Hayward St. Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
We are trying to localize iron in yeast with light and electron microscopy . Has anybody know of stains for iron at the light microscope and also for electron microscope.
We would appreciate any input and suggestions you may have. Thanks for your help, Gilles
If there is anyone out there who has recently designed a new EM lab in new construction, I would appreciate hearing what special instructions you gave to the architects and engineers to assure the appropriate environmental conditions for operation of the instruments.
Thanks, Greg Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, ICBR EM CORE University of Florida Ph. 352-392-1295 PO Box 118525 Fax 352-846-0251 Gainesville, FL 32611 http://www.biotech.ufl.edu/EM
If there is anyone out there who has recently designed a new EM lab in new construction, I would appreciate hearing what special instructions you gave to the architects and engineers to assure the appropriate environmental conditions for operation of the instruments.
Thanks, Greg
Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, ICBR EM CORE University of Florida Ph. 352-392-1295 PO Box 118525 Fax 352-846-0251 Gainesville, FL 32611 http://www.biotech.ufl.edu/EM
1) Deconvolution. This technique takes several images (a stack) of images taken at different focus positions, and calculates the "blurring" in each image and finally removes it for a focused image. There are different types of deconvolution (no neighbor, nearest neighbor, blind iterative). These algorithms are fairly computation intense and can take a while to complete, especially on large images. You also need information about the microscope to get the best results.
2) Extended Focal Imaging (we call it that way, other manufacturers have different names). This is a simpler approach, where the software simply "collects" the focused parts from each image and combines them into a new image. This usually works faster than deconvolution and you don't need any other information from the microscope, but there might be artifacts in areas where you find no structure on the image.
If you want to get more information, you can check "deconvolution" on the internet, or go to our web site and look for "ride" (rapid image deconvolution) and EFI (Extended Focal Imaging). As I mentioned above, other manufacturers have similar software.
mike
} } } } } } } } } } WE HAVE MOVED { { { { { { { { { please make a note of the new address below
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Ann-Fook Yang [mailto:yanga-at-agr.gc.ca] Sent: Tuesday, July 09, 2002 2:47 PM To: microscopy-at-sparc5.microscopy.com
My colleague asks: Is it true or is a hoax that there is a software which makes it possible to get focussed light microscopy images from pairs of under- and overfocussed micrographs? If it si true, what is the name of the software and where is it available? Thank you. M. Kalab
I tried a lot of stuff to solve my "pepper" problem either on the "stainig side", like shorter lead stain, different batch of lead citrate, prolonged washes after fix and osmium, I don´t use phosphate buffers, etc, etc or on the scope side (Philips EM 201) like using different objektive/condensor apertures, low kv, low emission, etc. etc. Nothing helped.
If I take a section I worked with a couple of weeks ago (and then it was ok in the Philips EM 20, I took dozens of nice negs) and put it in the same Philips EM 201 now (exactly the same scope settings) I get this "pepper" and it is really bad. If I take such a "peppered" section to the Zeiss EM 10 I still can see the "pepper", so it is not a problem of not being able to see the contamination in the Zeiss.
It drives me crazy.
Stefan
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° Stefan Geimer MCDB Dept. Yale University P.O. Box 208103 New Haven, CT 06520-8103 U.S.A.
But do you have the problem with a section that is taken from the Philips to the Zeiss?
Thanks,
Fred Monson
} ---------- } From: Stefan Geimer } Sent: Tuesday, July 9, 2002 2:04 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: TEM, Epon sections, problem with "pepper" } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have the following problem: } I am observing Epon sections, stained with uranyl acetate and lead } citrate in an Philips EM 201. The sections show a really severe } contamination with electron dense grains. These grains are always } associated with embedded structures (membranes, microtubules etc.).The } problem started after I changed the cathode. The contamination looks } kinda like lead-stain granularity and I think it has something to do } with the lead. A section stained with uranyl acetate only looks fine } (but I need the lead to get enough stain). } The crazy thing is that I don´t have the problem when I use our other } scope, a Zeiss EM 10. So if I take one of my sections and have a look at } it in the Zeiss EM 10, everything is perfect. The same section observed } in the Philips EM 201 shows this contamination with electron dense } grains. Both scopes operated at comparable conditions (80 kv, cold trap } etc.). So the contamination seems to be lead citrate and scope } dependend. } Anybody ever had that problem and might have an idea how to solve it? } } Stefan } } } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } Stefan Geimer } MCDB Dept. } Yale University } P.O. Box 208103 } New Haven, CT 06520-8103 } U.S.A. } } Tel.: 203/432-3473 } Fax.: 203/432-6210 } } e-mail: stefan.geimer-at-yale.edu } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } } }
I'm not sure what your design goals are, but the following may give you some ideas...
We designed a lab, with focus on atom-resolution TEM and SPM, to occupy half of the first floor of a 3 story building. There was a writeup about it in the November 1999 issue (vol 4 no 11) of Laboratory Design. A brief quote from the article said: "After reviewing geotechnical studies, designers decided to place the electron microscopy lab on the first floor, as far away as possible from the arterial road. Mechanical equipment, as well as the microscope's generator, were located on the first floor in the office wing, which is separated from the lab block by a 2-in. seismic joint. A second 2-in. expansion joint isolates the lab wing from the adjacent Benton Hall. The microscopy lab rests on a dedicated 2-ft-thick concrete foundation, with theater-wall construction further mitigating vibration." I'm not sure what they mean here by "the microscope's generator", but the elastic barriers they describe extend through all 3+1 floors of the facility.
Electrical wiring and air handling systems were designed to minimize stray fields and asymmetric convection around electron microscope columns, separate air conditioning controls supplied the HREM room (allowing shut-down to test it's effects on vibration if necessary) along with a ceiling crane for column disassembly and a wall feedthrough allowing the scope's low voltage power supplies to be located in an adjacent, vibrationally separated, area. Hall closets for chillers, transformers, etc. were located well away from more sensitive instruments. Dust generating areas (e.g. specimen prep) were located away from the scope rooms, and specimen handling areas were given single-color floor tiles to make small dropped objects easier to find.
Lastly, since we knew we couldn't do much after the fact if building design goals were compromised during construction, we made it a practice to attend weekly construction meetings, and to schedule vibration isolation tests at appropriate times during construction. I remember painfully one in particular, when the only way we could figure how to measure vibration attenuation between the 2-foot thick slab, and the newly- poured floor surrounding, was for me to jump down from a 4-foot retaining wall onto the new floor hitting with maximum impact (that was the bad part) while others monitored on-slab and off-slab vibration. We got numbers for amplitude attentuation (I think somewhere between a factor of 10 and 100, in the 10 Hz frequency range), but the next day I could barely get out of bed. Of course, it was less the results of the tests than the fact that everyone knew we were actively doing them that, we felt, worked in our favor.
Most elements of the strategy worked (provided I don't mention light leaks in the darkroom). Under normal contact mode operating conditions sitting out in the room on a vibration table, our Nanoscope III SPM has stationary-tip vibration amplitudes of about half an Angstrom, and our 300 kV Philips SuperTwin continues to reliably deliver contrast in the sub-2A range even on amorphous materials using the lowest possible bias setting. If you have further questions or would like more details, contact me off of the list server.
Cheers. /pf
Phil Fraundorf UM-StL Physics and Astronomy St. Louis MO 63121 office: (314)516-5044 lab: (314)516-5024 fax: (314)516-6152 http://www.umsl.edu/~fraundor
*********** REPLY SEPARATOR ***********
On 7/10/2002 at 9:00 AM you? wrote:
} If there is anyone out there who has recently designed a new EM lab in new } construction, I would appreciate hearing what special instructions you } gave } to the architects and engineers to assure the appropriate environmental } conditions for operation of the instruments. } } Thanks, Greg } } Gregory W. Erdos, Ph.D. } Assistant Director, Biotechnology Program } Scientific Director, ICBR EM CORE } University of Florida Ph. 352-392-1295 } PO Box 118525 Fax 352-846-0251 } Gainesville, FL 32611 http://www.biotech.ufl.edu/EM
Hi Stephan, I haven't yet worked (soon but not yet, so I can't tell you much about protocoles) with IgY but I can tell you where to buy secondary Ab to IgY conjugated to gold: BIO/CAN Scientific (Jackson) 1-800-387-8125 or 905-828-2455 They are located in Ontario, Canada. Donkey anti-chicken IgY - 6 nm cat# 703--195-155 Donkey anti-chicken IgY - 12 nm cat# 703--205-155 Donkey anti-chicken IgY - 18 nm cat# 703--215-155 Hope it helps
} Hi Stephan, } I haven't yet worked (soon but not yet, so I can't tell you much about protocoles) with IgY but I can tell you where to buy secondary Ab to IgY conjugated to gold: BIO/CAN Scientific (Jackson) 1-800-387-8125 or 905-828-2455 } They are located in Ontario, Canada. } Donkey anti-chicken IgY - 6 nm cat# 703--195-155 } Donkey anti-chicken IgY - 12 nm cat# 703--205-155 } Donkey anti-chicken IgY - 18 nm cat# 703--215-155 } Hope it helps } } Emmanuelle } } } } Emmanuelle Roux, PhD } Senior Scientist } Caprion Pharmaceuticals } 7150 Alexander Fleming } St-Laurent, H4S 2C8 } Quebec, Canada } Tel: 514-940-3600 ext. 3773 } Fax: 514-940-3620 }
I have a little problem. I am staining thin sections of LR White embedded plant tissue with a 1% aqueous solution of Congo Red (Sigma) for 1 min, with three 'copious' washes from a distilled water wash bottle. Instead of the cell wall labelling, I get a 'negative' image of the wall, and the cytoplasm stains strongly. I don't know why this is so; I made up a mew batch of the stain, but that didn't improve the labelling, and it's never happened before. I checked the pH, and it's around 9. Am I going mad, or is there something about Gongo Red that I should know about?
Thanks for you help in advance,
Mark.
Mark Talbot Department of Biological Sciences University of Newcastle Callaghan NSW 2308 AUSTRALIA
I am curious to hear the answer to this question from Jacques and I have something to add. Once I performed EDS analyses on a sample of BC particles on C film in order to test a new EDS analysis system installed on a new FEG 200KeV TEM. After selecting the correct time constant in the EDS software I could get nice distinguishable C and B peaks. Later I tried doing a similar EDS analysis on large FeB2 particles in
a Fe matrix. I mention large here to stress that the particles went through the specimen foil so most of the X-rays were being emitted directly from the particles
without passing through the Fe matrix before reaching the detector. I never saw even a hint of a B peak. These ppts should have contained 33at%B. Was I doing something wrong or is that an indication of how easily boron X-rays are absorbed within a sample containing large z atoms?
Faerber Jacques wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi all } } In the same idee, what about mesurements on boron carbide ? Carbon AND } boron concentrations ! I see nice spectras, without anything else ( a bit } O, some times Al and N). And I have variations between samples in the B/C } ratio. In such a case can I mesure only ratio variation, or is it possible } to try some quantification (with standards). The sample is bulk B4C } and laser ablation thick layers (with dropplets). Primary energy 3 to 5 } keV. } } I think it's a bit pretentious to quantify. What is other's opinion ? } } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess } 67037 Strasbourg CEDEX } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)0 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } On Tue, 9 Jul 2002, Ronald Vane wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Dear Chris and all: } } } } Quantitative analysis of Carbon by EDX is nearly impossible because of the } } very shallow penetration depth of the Carbon X-rays. You really just measure } } the surface. Surface analysis techniques such as XPS and Auger also show } } that thin carbon films love to form on surfaces. If you have an XPS you use } } your ion gun to sputter off the carbon surface scum to see the real surface. } } } } Dry ashing in a plasma cleaner can also remove the carbon surface layer. } } Sputter etching can be used to remove gold and silver coatings. } } } } Ron Vane } } XEI Scientific } } 3124 Wessex Way, Redwood City, CA 94061 } } 650-369-0133 } } www.SEMCLEAN.com } } } } Note: XEI Scientific makes the EVACTRON plasma cleaning system for Electron } } Microscope Chambers and FIBs, but does not make desk top plasma dry ashers } } or sputter etchers. } } } } -----Original Message----- } } } From: Chris Peppiatt {chris.peppiatt-at-nuigalway.ie} } } To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com} } } Date: Monday, July 08, 2002 3:22 PM } } Subject: Carbon Quantitation by EDX } } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } Dear All, } } } } } } Someone has asked me to do some work for them on mineral specimens. The } } } samples will be mounted in resin and polished and we will then coat with } } } carbon. Obviously we will set our software to deconvolute carbon from the } } } analyses. We have the option to coat samples with either gold or silver and } } } then look at the carbon content. I suppose the questions I would like to } } } have answered are: } } } } } } (1) Heavy elements like gold or silver will absorb some of the light } } } element (inc. carbon) X-rays when used as coatings. Is there any way of } } } correcting for this to get an accurate quantitative analysis of carbon } } content? } } } } } } (2) Is there any way of removing either gold/silver or carbon coatings from } } } such samples except for the obvious method of regrinding/polishing the } } } coating off? } } } } } } Thanks in advance. } } } } } } Regards, } } } } } } Chris Peppiatt } } } } } } } } } ============================================ } } } Dr. Chris Peppiatt (Experimental Officer), } } } The National Centre for Biomedical Engineering Science, } } } Science & Engineering Technology Building, } } } National University of Ireland Galway, } } } Galway City, Co. Galway, } } } Republic of Ireland. } } } chris.peppiatt-at-nuigalway.ie } } } Phone: +353 91 512157 Fax: +353 91 750596 } } } ============================================= } } } } } } } } } } } } }
-- Dr. Steven Celotto Department of Engineering Materials Science & Engineering University of Liverpool Brownlow Hill Liverpool L69 3BX UNITED KINGDOM
For the LM either "Prussian Blue" reaction or "Turnbull's Blue" reaction depending on whether your iron is +3 (ferric) or +2 (ferros). Theses are very simple and in any histotechnique text (ferro- or ferri- cyanide in HCl) . You should run a control for verification. At the EM level I would think that iron deposits would be electron dense and not require any staining. In fact, they might be more visible in an unstained section.
Gilles Grondin wrote:
} We are trying to localize iron in yeast with light and electron microscopy } . Has anybody know of stains for iron at the light microscope and also for } electron microscope. } } We would appreciate any input and suggestions you may have. Thanks for your } help, } Gilles
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
I have to ask the obvious, just to make sure that your staining technique is meticulous.
1. are you doing the lead citrate stain in a low Co2 environment? KOH, sucks up CO2 a lot better than NaOH... so try that... in a glass petri dish.
2. Use ultra clean boiled water to make up the lead citrate stain to drive out CO2.
3. Wash the living hell out of the grids after staining. I bang them up and down 60 times in half a liter of ultra pure water in 3 separate beakers, after every stain. [I can stain about 35 grids on one of those rubber grid holders with the slits in them to hold the grids... they are nice. You can cut more slits to hold even more grids.]
4. Never use the lead citrate near the bottom of the vial. Every time I try that, I end up with peppering, and make sure that your stain is fresh.
The reason that you don't see the artifact in one microscope probably just means that you dont' have the same contrast between the 2 machines... maybe different obj. aperture sizes or a lot of other reasons. But trust me, it's probably still there.
I have to ask the obvious, just to make sure that your staining technique is meticulous.
1. are you doing the lead citrate stain in a low Co2 environment? KOH, sucks up CO2 a lot better than NaOH... so try that... in a glass petri dish.
2. Use ultra clean boiled water to make up the lead citrate stain to drive out CO2.
3. Wash the living hell out of the grids after staining. I bang them up and down 60 times in half a liter of ultra pure water in 3 separate beakers, after every stain. [I can stain about 35 grids on one of those rubber grid holders with the slits in them to hold the grids... they are nice. You can cut more slits to hold even more grids.]
4. Never use the lead citrate near the bottom of the vial. Every time I try that, I end up with peppering, and make sure that your stain is fresh.
The reason that you don't see the artifact in one microscope probably just means that you dont' have the same contrast between the 2 machines... maybe different obj. aperture sizes or a lot of other reasons. But trust me, it's probably still there.
I seem to recall reading somewhere about a method of removing the top cell membranes from a cell culture layer. It seems to have involved placing a layer of material over the cultured cells and stripping it away, but I can't remember anything about where I saw the reference.
Can somebody help jog my memory? Or did I dream this? I figure if ANYONE knows, you folks will!
Thanks!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
Has anyone out there ever silver enhanced 20nm gold particles? I would like to see by light microscopy the distribution in rat lung of inhaled gold particles. I have done 1nm gold particle enhancement but that was for EM viewing. Would it involve the same methodology?
Thanks!
Karen L. Bentley, M.S.(previously Jensen) Associate Scientist & Project Manager Electron Microscope Research Core University of Rochester Medical Center Rochester, NY 14642 585-275-1954
I have to ask the obvious, just to make sure that your staining technique is meticulous.
1. are you doing the lead citrate stain in a low Co2 environment? KOH, sucks up CO2 a lot better than NaOH... so try that... in a glass petri dish.
2. Use ultra clean boiled water to make up the lead citrate stain to drive out CO2.
3. Wash the living hell out of the grids after staining. I bang them up and down 60 times in half a liter of ultra pure water in 3 separate beakers, after every stain. [I can stain about 35 grids on one of those rubber grid holders with the slits in them to hold the grids... they are nice. You can cut more slits to hold even more grids.]
4. Never use the lead citrate near the bottom of the vial. Every time I try that, I end up with peppering, and make sure that your stain is fresh.
The reason that you don't see the artifact in one microscope probably just means that you dont' have the same contrast between the 2 machines... maybe different obj. aperture sizes or a lot of other reasons. But trust me, it's probably still there.
the following is copied from our HR group announcement. The official bits are listed below. I can answer some questions but I'm not the decision maker so please don't bombard me with emails!
cheers, JSV ******************
Would you like to work at a National Laboratory? The Pacific Northwest National Laboratory is looking for a Microscopist. If you are interested, please apply by visiting our website: http://jobs.pnl.gov/jobs.asp?req=104165 PNNL is an EEO/AA employer and values diversity in the workplace. F/M/D/V are encouraged to apply.
Materials Interfaces and Characterization Materials Science Division Science & Engineering Associate II Min. Salary: 40K/yr - Max. Salary: 59K/yr (salary is commensurate with education and experience).
This position requires experience in electron microscopy and a 4-year degree in a field of science or its equivalent. Background should be in metal and ceramic sample preparation and in the operation of transmission and scanning electron microscopes. Working experience with the use of analytical electron microscopes is highly desired. Background in the handling, preparation and examination of materials is also required. Will lead technical activities dealing with material preparation and characterization for PNNL scientists and engineers in support of a variety of projects within the technical group. Will be responsible for performing analytical characterization of materials, using transmission and scanning electron microscopy. Will contribute research data for publications in refereed technical journals and in reports to various sponsors. Excellent oral and written communication skills and ability to establish positive working relationships with other technical staff is required. This position will report to the Materials Interfaces and Characterization Technical Group Manager. ***********************
******** John S. Vetrano Sr. Research Scientist Pacific Northwest National Laboratory MSIN P8-16 P.O. Box 999 Richland, WA 99352 Phone: (509)372-0724 Fax: (509)376-6308 Email: mailto:john.vetrano-at-pnl.gov
} Hi all; } } the following is copied from our HR group announcement. The official bits } are listed below. I can answer some questions but I'm not the decision } maker so please don't bombard me with emails! } } cheers, JSV } ****************** } } Would you like to work at a National Laboratory? The Pacific Northwest } National Laboratory is looking for a Microscopist. If you are interested, } please apply by visiting our website: } http://jobs.pnl.gov/jobs.asp?req=104165 } PNNL is an EEO/AA employer and values diversity in the workplace. F/M/D/V } are encouraged to apply. } } Materials Interfaces and Characterization } Materials Science Division } Science & Engineering Associate II } Min. Salary: 40K/yr - Max. Salary: 59K/yr (salary is commensurate with } education and experience). } } This position requires experience in electron microscopy and a 4-year } degree in a field of science or its equivalent. Background should be in } metal and ceramic sample preparation and in the operation of transmission } and scanning electron microscopes. Working experience with the use of } analytical electron microscopes is highly desired. Background in the } handling, preparation and examination of materials is also required. Will } lead technical activities dealing with material preparation and } characterization for PNNL scientists and engineers in support of a variety } of projects within the technical group. Will be responsible for } performing analytical characterization of materials, using transmission } and scanning electron microscopy. Will contribute research data for } publications in refereed technical journals and in reports to various } sponsors. Excellent oral and written communication skills and ability to } establish positive working relationships with other technical staff is } required. This position will report to the Materials Interfaces and } Characterization Technical Group Manager. } *********************** } } } ******** } John S. Vetrano } Sr. Research Scientist } Pacific Northwest National Laboratory } MSIN P8-16 } P.O. Box 999 } Richland, WA 99352 } Phone: (509)372-0724 Fax: (509)376-6308 } Email: mailto:john.vetrano-at-pnl.gov }
If anyone out there has a Kevex Analyst 8000 in "excess storage" we would be interested in a pulse processor (4460). Who knows? We may be looking for other modules in the near future. Please contact me offline.
Warm Regards, Annie --
+++++++++++++++++++++++++++++
R. Ann Bliss Technician, Chemistry and Materials Science Materials Science and Technology Division Lawrence Livermore National Laboratory
We have a TMC isolation platform for a TEM that we wish to sell as our new scope will not fit on this platform. Currently used for a JEOL 100C. Unit purchased in 1994 and in excellent condition. Interested parties should contact
Joe Goodhouse Confocal / EM Core Laboratory Manager Department of Molecular Biology Princeton University 609-258-5432 jgoodhouse-at-molbio.princeton.edu
Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
HI Randy, We've done this with cell cancer lines incubated onto a bone surface or with bacteria incubated on a polished planchet of hornblende. I placed double stickey C tabs cut into thin slivers onto an alumium stub, remove the tab cover, inverted the aluminum, stub with the exposed sticky +conductive surface over a dried /coated and previously imaged sample, touching it lightly to the surface before pulling it away---the idea being to Au/Pd coat both stubs, and then check them for the cells, cell content or for the pit formed when the cells anchored into the layer of bone / hornblende. As might be expected, I had fewer problems with the hornblende surface than with the bone slice. I hope that your ultrasmall gold course went well. Rosemary
.At 02:38 PM 7/10/02 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Haskris Water to Air Chiller, puchased in 1994 to cool one Jeol 840 SEM and a Jeol 100C TEM, from 1994 to 1996. 1996 to present only used for the TEM. Unit is in very good condition. Asking $20000.00 or Best offer plus removal and shipping costs. Contact
Joe Goodhouse Confocal / EM Core Laboratory Manager Department of Molecular Biology Princeton University 609-258-5432 jgoodhouse-at-molbio.princeton.edu
Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
on 7/9/02 7:37 PM, Allan Mitchell at allan.mitchell-at-stonebow.otago.ac.nz wrote:
} } Hi Wilbur, Thanks for the update } } The recirculating cooling water systems problem that we have the most } problem trying to get a handle on is controlling the pH of the } cooling water to prevent corrosion. Since the end of the good old } days when we were allowed to use Sodium Chromate and Sodium } Bicarbonate we have not yet found a satisfactory replacement. } } Satisfactory includes; effectiveness at pH control, life time before } depletion, safe handling and disposal, cost, availablility. } } Trying to get good advice also seems to like the proverbial hens teeth. } } Anyone out there come up with any good products recently. } Dear Allan, Back at the HVEM, we used Aqua Treet 42 for anti-corrosion and adjusted the pH to between 8.0 and 8.5 with NaOH. The Aqua Treet--from Aqua Labs somewhere in NJ as I recall--works well at that pH. We checked the concentration with a color kit once each month, and had to add more only occasionally; it is very safe (I didn't drink any, but it is not corrosive, etc.); the cost was under $100 for a 5 gal tub, which has lasted for many years, and is nowhere near the bottom. Last time I checked, Aqua Labs was reachable by phone and was on the web. Good luck. Yours, Bill Tivol
on 7/9/02 7:46 PM, rcmoretz-at-att.net at rcmoretz-at-att.net wrote:
} Altho' not directly involved in the } work, I do remember that the fixative was hydrogen } sulfide saturated glutaraldehyde (really noxious mixture- } -in more ways than one!). There is also some literature } related to Cd localization, but I can't dredge up } names. } Roger Moretz } -- } Where the world is only slightly } less weird than it actually is.
Dear Roger, Sulfide should precipitate Cd as well as Zn. Yours, Bill Tivol
Randy There was a presentation at a South African EM Society meeting in 1983 on this subject: Hughes, F & Rijkenberg, F H J - 1983: An epidermis removal technique for studying infection processes of Puccinia sorghi in Maize leaves. Proc. Electron Microsc. Soc. South Afr. 13, 17-18.
The basics are: Fix and dehydrate specimens, then CPD, mount onto SEM stub. A second stub, onto which double-sided sticky tape has been affixed, is pressed to the specimen surface and pulled away. After coating both stubs, the inner and outer surfaces of the specimen can be studied.
If required, I can fax you a copy of the two pages.
Regards Jan Coetzee
"Tindall, Randy D." wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear listers, } } I seem to recall reading somewhere about a method of removing the top cell membranes from a cell culture layer. It seems to have involved placing a layer of material over the cultured cells and stripping it away, but I can't remember anything about where I saw the reference. } } Can somebody help jog my memory? Or did I dream this? I figure if ANYONE knows, you folks will! } } Thanks! } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-5414 } Email: tindallr-at-missouri.edu } Web: http://www.biotech.missouri.edu/emc/
-- Jan Coetzee Lab for Microscopy and Microanalysis University of Pretoria, South Africa. Tel: 012-420-2075, Fax 012-362-5150 www.up.ac.za/academic/electron/emunit1.htm
Some weeks ago, there was a discussion about the effects of pH and many other factors on the stainings (in that case toluidine blue). The conclusion was, if I can say, that we can never know ...., only try.
Regarding your problem, I had a similar experiment with ruthenium red on JB4 embedded European beeches sections. At neutral and basic pH, the stain is in the cytoplasm (or in the vacuoles?). At pH {= 5, the pectins (near the walls) stained. It remained me an experiment I did on practical work (when I was student) in which we experimented that the staining of the walls migrates to the vacuols when the pH is changed from acidic to basic...
You can try quickly the same with your sections:
1) stain them as you do 2) rince them with an acidic buffer or solution and mount them in that buffer (non permanent mounting)
3) look at them, perhaps you will see after some time that the staining in, or near the walls
4) if it works, one conclusion would be : the pH of rinsing is as (or perhaps in some cases) more important than the pH of the staining itself. But I stop here, because I'm sure there will be many other opinions...
Another problem could perhaps be that embedding was not optimal (did you your staining on the same sections than when it worked ?)...
Hope it hels
Chris Wuethrich Beth Israel Deaconess medical Center 330, Brookline AVE Boston, MA 02215
PCan someone please explain why the solutions still concentrate on the stain and its condition rather than taking the tack that the problem lies with the Philips scope?
Fred Monson
} ---------- } From: Monson, Frederick C. } Sent: Wednesday, July 10, 2002 11:28 AM } To: 'Stefan Geimer' } Cc: 'List-Microscopy' } Subject: RE: TEM, Epon sections, problem with "pepper" } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } But do you have the problem with a section that is taken from the Philips } to } the Zeiss? } } Thanks, } } Fred Monson } } } ---------- } } From: Stefan Geimer } } Sent: Tuesday, July 9, 2002 2:04 PM } } To: Microscopy-at-sparc5.microscopy.com } } Subject: TEM, Epon sections, problem with "pepper" } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I have the following problem: } } I am observing Epon sections, stained with uranyl acetate and lead } } citrate in an Philips EM 201. The sections show a really severe } } contamination with electron dense grains. These grains are always } } associated with embedded structures (membranes, microtubules etc.).The } } problem started after I changed the cathode. The contamination looks } } kinda like lead-stain granularity and I think it has something to do } } with the lead. A section stained with uranyl acetate only looks fine } } (but I need the lead to get enough stain). } } The crazy thing is that I don´t have the problem when I use our other } } scope, a Zeiss EM 10. So if I take one of my sections and have a look at } } it in the Zeiss EM 10, everything is perfect. The same section observed } } in the Philips EM 201 shows this contamination with electron dense } } grains. Both scopes operated at comparable conditions (80 kv, cold trap } } etc.). So the contamination seems to be lead citrate and scope } } dependend. } } Anybody ever had that problem and might have an idea how to solve it? } } } } Stefan } } } } } } } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } Stefan Geimer } } MCDB Dept. } } Yale University } } P.O. Box 208103 } } New Haven, CT 06520-8103 } } U.S.A. } } } } Tel.: 203/432-3473 } } Fax.: 203/432-6210 } } } } e-mail: stefan.geimer-at-yale.edu } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } } } } } } } } }
Check the work of Richard G Anderson (Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas).
One example of publication which I think described that technique : Moore MS, Mahaffey DT, Brodsky FM, Anderson RG. Assembly of clathrin-coated pits onto purified plasma membranes. Science 1987 May 1;236(4801):558-63.
"Tindall, Randy D." wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear listers, } } I seem to recall reading somewhere about a method of removing the top cell membranes from a cell culture layer. It seems to have involved placing a layer of material over the cultured cells and stripping it away, but I can't remember anything about where I saw the reference. } } Can somebody help jog my memory? Or did I dream this? I figure if ANYONE knows, you folks will! } } Thanks! } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-5414 } Email: tindallr-at-missouri.edu } Web: http://www.biotech.missouri.edu/emc/
-- Marc Pypaert Department of Cell Biology, Center for Cell and Molecular Imaging and Ludwig Institute for Cancer Research, Yale University School of Medicine 333 Cedar Street New Haven CT 06520 Tel : (203) 785 3681 Fax : (203) 785 7446
I plan to upgrade my film scanner for TEM 3.25x4 in. negatives. (Currently I am using Microtek ScanMaker 8700 and Agfa Duoscan T2500). I have located two scanners that fit into my requirement. One is Minolta Dimage Scan multi Pro, the other one is Nikon Super Coolscan 8000ED. Minolta scanner has a slightly higher optical resolution (4800 dpi) and Dynamic range of 4.8.
Can anybody who has experience with these scanners help me to make a decision? Thanks a lot.
Xinran Liu
Xinran Liu, M.D., Ph.D. Center for Basic Neuroscience UT Southwestern Medical Center at Dallas Phone: 214-648-1830 Fax: 214-648-1801 E-mail: xinran.liu-at-utsouthwestern.edu
The reason why I took this position is because I have sometimes seen peppering as a result of faulty staining technique with lead citrate. I have not see this sort of "peppering" as a result of microscope contamination. In the case of microscope contamination, it shows more as a general density increase over a specific area of the grid....like a burn.
That said, I've never used a Philips EM, but I have used Jeol 1010, Hitachi 7000, AEI 6B and AEI 801, Zeiss 109, and a Zeiss 10CR and an old Zeiss 9 I believe. In my 18 years of experience, I've never seen peppering as a result of a microscope contamination. It doesn't mean that it can't happen, just that in my experience, I haven't seen it. I can only talk about what I've seen, or haven't seen.
PCan someone please explain why the solutions still concentrate on the stain and its condition rather than taking the tack that the problem lies with the Philips scope?
Fred Monson
} ---------- } From: Monson, Frederick C. } Sent: Wednesday, July 10, 2002 11:28 AM } To: 'Stefan Geimer' } Cc: 'List-Microscopy' } Subject: RE: TEM, Epon sections, problem with "pepper" } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } But do you have the problem with a section that is taken from the Philips } to } the Zeiss? } } Thanks, } } Fred Monson } } } ---------- } } From: Stefan Geimer } } Sent: Tuesday, July 9, 2002 2:04 PM } } To: Microscopy-at-sparc5.microscopy.com } } Subject: TEM, Epon sections, problem with "pepper" } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I have the following problem: } } I am observing Epon sections, stained with uranyl acetate and lead } } citrate in an Philips EM 201. The sections show a really severe } } contamination with electron dense grains. These grains are always } } associated with embedded structures (membranes, microtubules etc.).The } } problem started after I changed the cathode. The contamination looks } } kinda like lead-stain granularity and I think it has something to do } } with the lead. A section stained with uranyl acetate only looks fine } } (but I need the lead to get enough stain). } } The crazy thing is that I don´t have the problem when I use our other } } scope, a Zeiss EM 10. So if I take one of my sections and have a look at } } it in the Zeiss EM 10, everything is perfect. The same section observed } } in the Philips EM 201 shows this contamination with electron dense } } grains. Both scopes operated at comparable conditions (80 kv, cold trap } } etc.). So the contamination seems to be lead citrate and scope } } dependend. } } Anybody ever had that problem and might have an idea how to solve it? } } } } Stefan } } } } } } } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } Stefan Geimer } } MCDB Dept. } } Yale University } } P.O. Box 208103 } } New Haven, CT 06520-8103 } } U.S.A. } } } } Tel.: 203/432-3473 } } Fax.: 203/432-6210 } } } } e-mail: stefan.geimer-at-yale.edu } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } } } } } } } } }
Haskris Water to Air Chiller, purchased in 1994 to cool one Jeol 840 SEM and a Jeol 100C TEM, from 1994 to 1996. 1996 to present only used for the TEM. This is a Model R150 Unit. 208/230 volts , 1 Phase 60 Hertz. Condenser is 1.75 HP. with lbs. refrigerant R-22 charge ,1/3 HP water pump with a 14 gallon tank. Unit is in very good condition. Asking $2,000.00 or Best offer plus removal and shipping costs. Contact
Joe Goodhouse Confocal / EM Core Laboratory Department of Molecular Biology Princeton University 609-258-5432 jgoodhouse-at-molbio.princeton.edu
Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (agodl-at-o2.pl) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday, July 11, 2002 at 11:30:16 ---------------------------------------------------------------------------
Email: agodl-at-o2.pl Name: Godlewski Andrzej
Education: Graduate College
Location: Medical University of Lodz, Lodz, Poland
Question: Hallo, In the autometallography the Timm method is used for revealing cations (eg zinc, cobalt)in different biological materials. The idea of This method is simply from chemical point of view: treatment of sample with Na2S (high pH)[sulphide of cation present in sample], wash, next AgNO3 in H2O (1%?)[substitution by silver other cattion ] and photographic developer [for silver revealing].The method is simply, accurate but not specific. The question: What is original recipe of Timm method? Best regard A.Godlewski MD PhD D Sci
Electron Microscopist Electron Probe Instrumentation Center (EPIC) Northwestern University, USA
Job description:
Research, collaboration and training of students and users of EPIC in all aspects of electron microscopy: particularly specimen preparation and FIB, SEM and TEM analysis.
Specific duties include:
(1) Teach, help and actively collaborate with users in preparing TEM/SEM samples and their observation by SEM/TEM. (2) Assist and conduct laboratory teaching in UG and graduate courses related to specimen preparation and electron microscopy. (3) Develop collaborative and independent research projects and topics related to advanced electron microscopy and nanostructures. (4) Maintain and develop sample preparation laboratory and equipment such as FIB, ultramicrotome, IBTs, PIPS, electrojet polishers, cutting saw, wire saw, grinder, vacuum evaporator, sputter coater, liquid nitrogen and other accessories. (5) Help maintain and develop computer facility within EPIC.
Qualifications and Needs:
A technical degree in physical science/engineering or bioscience is needed. Actual hands-on experience in specimen preparation of hard and soft materials, and electron microscopy (SEM/TEM) is required. Need to be familiar with modern computers and basic user programs. Laboratory teaching experience is highly desirable.
Excellent prospects for personal and professional growth.
Send Resume and 3 References directly to:
Prof. Vinayak P. Dravid Materials Science & Engineering Director, Electron Probe Instrumentation Center (EPIC) 2225 N. Campus Drive, 1133 MLSF Northwestern University, Evanston, IL 60208, USA Ph.: (847) 467-1363, Fax: (847) 467-6573 E-mail: v-dravid-at-northwestern.edu http://vpd.ms.northwestern.edu http://epic.ms.northwestern.edu **********************************************************
Electron Microscopist Electron Probe Instrumentation Center (EPIC) Northwestern University, USA
Job description:
Research, collaboration and training of students and users of EPIC in all aspects of electron microscopy: particularly specimen preparation and FIB, SEM and TEM analysis.
Specific duties include:
(1) Teach, help and actively collaborate with users in preparing TEM/SEM samples and their observation by SEM/TEM. (2) Assist and conduct laboratory teaching in UG and graduate courses related to specimen preparation and electron microscopy. (3) Develop collaborative and independent research projects and topics related to advanced electron microscopy and nanostructures. (4) Maintain and develop sample preparation laboratory and equipment such as FIB, ultramicrotome, IBTs, PIPS, electrojet polishers, cutting saw, wire saw, grinder, vacuum evaporator, sputter coater, liquid nitrogen and other accessories. (5) Help maintain and develop computer facility within EPIC.
Qualifications and Needs:
A technical degree in physical science/engineering or bioscience is needed. Actual hands-on experience in specimen preparation of hard and soft materials, and electron microscopy (SEM/TEM) is required. Need to be familiar with modern computers and basic user programs. Laboratory teaching experience is highly desirable.
Excellent prospects for personal and professional growth.
Send Resume and 3 References directly to:
Prof. Vinayak P. Dravid Materials Science & Engineering Director, Electron Probe Instrumentation Center (EPIC) 2225 N. Campus Drive, 1133 MLSF Northwestern University, Evanston, IL 60208, USA Ph.: (847) 467-1363, Fax: (847) 467-6573 E-mail: v-dravid-at-northwestern.edu http://vpd.ms.northwestern.edu http://epic.ms.northwestern.edu **********************************************************
This is my experience with Minolta Scan Multi Pro film scanner. After a couple of months using it I had to adapt the way I take my TEM pictures to the scanner. The reason is that the 8x10cm negatives can't be rotated even using the multi format film holder. And the largest area it can scan is something around 6x9cm. If you are scanning a small area in the negative this in not a problem. But in my case some of the pictures I take is at low mag (less than 5k), and cover almost the entire negative. What I do is choose a magnification that can fit everything of interest in the "scannable" area. About the dynamical range, I still don't see much difference between a negative scanned using the Minolta film scanner or the old Epson tabletop scanner we have (supposed to be 3.0). But maybe it is just a matter of getting used to the new scanner. BTW, if you download the manual of this scanner from Minolta, you will notice in the Specification section a notice about the Dynamical Range, "4.2 (tested value)". Maybe this 4.8 is obtained under some "special" condition. :P
The Nikon one should not be much different, I suppose. :)
Regards,
Carlos
On Thu, 11 Jul 2002, Xinran Liu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Colleagues, } } I plan to upgrade my film scanner for TEM 3.25x4 in. negatives. } (Currently I am using Microtek ScanMaker 8700 and Agfa Duoscan T2500). I } have located two scanners that fit into my requirement. One is Minolta } Dimage Scan multi Pro, the other one is Nikon Super Coolscan 8000ED. } Minolta scanner has a slightly higher optical resolution (4800 dpi) and } Dynamic range of 4.8. } } Can anybody who has experience with these scanners help me to make a } decision? Thanks a lot. } } Xinran Liu
o-------------------------------------------------------o | Carlos Kazuo Inoki | | Department of Physics - University at Albany | | 1400 Washington Ave.- Albany - NY - 12222 | o-------------------------------------------------------o
I could not imagine the situation when the SAME section will looks dirty in one microscope (does not matter Phillips or not) and perfectly fine in another... Only one explanation: the 'good' microscope is misaligned (sorry EM10) in such degree that you do see practically nothing (the dirty things becomes invisible). I am so sorry for such rude interpretation. I would more believe to the 'worse' microscope and will pay attention to the staining procedure to avoid precipitates.
Sergey
At 09:30 AM 7/11/02, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
I used a Philips 201 for a number of years and got great results as long as I did not dwell too long in a particular area or a single grid. However, I always used LN2 whenever I examined or photographed samples because of the issue associated with vacuum contamination. You could place a specimen in the field of view and literally watch the contamination particles form!
The 201 had a mercury/oil diffusion pump and although this may not have been the entire issue, it was a problem. The other issue was the age of the instrument. My question would be: how many filament hours are you getting out of the 201? This is frequently a choice method for determining scope operation and vacuum function.
I have used and am still using a Zeiss EM10CA since purchased new in 1986. In addition to the oil diffusion pump, the scope has twin getter pumps: I always use LN2 when viewing or imaging specimens. I can sit there for 15 minutes and never see contamination. This is true for tissue embedded in LR White, Epon, Epon substitutes, Spurrs, etc. Furthermore, I get an average of 300 hours on Zeiss supplied tungsten filaments: yes, this is true!!
So, Fred, I believe you are correct in your deduction!
Sincerely, Ken
------ Ken Tiekotter, The University of Portland Department of Biology 5000 N. Willamette Blvd. Portland, OR 97203 USA
Director, MicroImaging Center, G50 Legacy Portland Hosptials Legacy Holladay Park Medical Center 1225 NE 2nd Avenue Portland, OR 97232 USA
Tel.: 503-413-5391
On Thu, 11 Jul 2002, Monson, Frederick C. wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } PCan someone please explain why the solutions still concentrate on the stain } and its condition rather than taking the tack that the problem lies with the } Philips scope? } } Fred Monson } } } ---------- } } From: Monson, Frederick C. } } Sent: Wednesday, July 10, 2002 11:28 AM } } To: 'Stefan Geimer' } } Cc: 'List-Microscopy' } } Subject: RE: TEM, Epon sections, problem with "pepper" } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } But do you have the problem with a section that is taken from the Philips } } to } } the Zeiss? } } } } Thanks, } } } } Fred Monson } } } } } ---------- } } } From: Stefan Geimer } } } Sent: Tuesday, July 9, 2002 2:04 PM } } } To: Microscopy-at-sparc5.microscopy.com } } } Subject: TEM, Epon sections, problem with "pepper" } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } I have the following problem: } } } I am observing Epon sections, stained with uranyl acetate and lead } } } citrate in an Philips EM 201. The sections show a really severe } } } contamination with electron dense grains. These grains are always } } } associated with embedded structures (membranes, microtubules etc.).The } } } problem started after I changed the cathode. The contamination looks } } } kinda like lead-stain granularity and I think it has something to do } } } with the lead. A section stained with uranyl acetate only looks fine } } } (but I need the lead to get enough stain). } } } The crazy thing is that I don´t have the problem when I use our other } } } scope, a Zeiss EM 10. So if I take one of my sections and have a look at } } } it in the Zeiss EM 10, everything is perfect. The same section observed } } } in the Philips EM 201 shows this contamination with electron dense } } } grains. Both scopes operated at comparable conditions (80 kv, cold trap } } } etc.). So the contamination seems to be lead citrate and scope } } } dependend. } } } Anybody ever had that problem and might have an idea how to solve it? } } } } } } Stefan } } } } } } } } } } } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } } Stefan Geimer } } } MCDB Dept. } } } Yale University } } } P.O. Box 208103 } } } New Haven, CT 06520-8103 } } } U.S.A. } } } } } } Tel.: 203/432-3473 } } } Fax.: 203/432-6210 } } } } } } e-mail: stefan.geimer-at-yale.edu } } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } } } } } } } } } } } } } } } }
i wonder if there are people out there who already possess the improved Inlens detector for the LEO Gemini 15xx or the Zeiss DSM 982.
According to their sales people, the detection efficiency was increased by 250 .. 300% and the new the detector material is supposed to be "radian hard" ... so far that is what they told us - Sounds to good to be true?
We would like to get hold of comparable user-image material which shows the same sample observed a)with your old and b)with the improved Inlens detector. (... parameter's like acquisition time, detector settings and so on should be provided if possible)
"Any" thougths, suggestions and experiences are welcome!
Dear Group - what would be the best procedure to prep a copper TEM grid with nanoparticles? What glue or adhesive would you suggest? I'm actually using a STEM holder (to get TEM image) on a SEM. Thanks Barb
New York Microscopical Society 30 North Mountain Avenue Montclair, NJ 07042
Bernard Friedman Memorial Workshop
Use of the Microscope September 21, 28, October 5, 12, 2002
A basic course on light microscopy which will cover the following topics: Theory of microscopy Kohler Illumination Diffraction Theory Contrast Methods Polarized light Phase Contrast Interference . . . . Hoffman contrast, Rheinberg, Dark-field & oblique Illumination, etc.
The workshop will consist of four consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of microscopy. The course instructors include Jan Hinsch of Leica, Inc., Dennis O’Leary of Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of SensIR Technologies, Inc. and N.Y.M.S. Instructor Don O'Leary.
WHEN: September 21, 28, October 5, 12, 2002 from 10 A.M. to 4 P.M.
WHERE: 30 N. Mountain Avenue, Montclair, NJ, Phone (973) 744-0043 ( parking available, accessible by public transportation. Information on car pools and transportation will be provided.)
COST: $325 for N.Y.M.S. members, $355 for non-members (includes membership) Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: Beginners and experienced users who wish to learn more about the proper use of a microscope.
HOW: Register using the form below. Limited to the first 12 registrants. Send form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.
FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 E-mail donoleary-at-worldnet.att.net Fax (425) 988-1415
PLEASE POST ---------------------------------------------------------------------------- ------------------- Registration Form Use of the Microscope 2002
Timing is sometimes NOT everything, BUT in this case it was a fault. My apology for the timing of my general question which was directed generally BUT in time appeared as a direct response to Garry's suggestion.
In any case, for those who address my question, including Garry, I appreciate the discussion and the enlightenment from those who have spent much more time in the driver's seat than I.
I often pose questions in a way that can cause others to bristle, but my questions are only intended to seek an answer. You see, I'm not certain what 'peppering' even refers to when one is discussing artifact on a 60-90nm section. I know that if I have such a problem a year from now that I will take the grid to the SEM to try to determine whether the contamination is in or on the section. On the other hand, if nothing else counts in this business, experience does, and I listen most intently to those with experience when they speak.
Respectfully yours,
Fred Monson
} ---------- } From: Garry Burgess } Sent: Thursday, July 11, 2002 12:30 PM } To: 'Monson, Frederick C.'; 'List-Microscopy' } Subject: RE: ???TEM, Epon sections, problem with "pepper" } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } The reason why I took this position is because I have sometimes seen } peppering as a result of faulty staining technique with lead citrate. I } have not see this sort of "peppering" as a result of microscope } contamination. In the case of microscope contamination, it shows more as } a } general density increase over a specific area of the grid....like a burn. } } That said, I've never used a Philips EM, but I have used Jeol 1010, } Hitachi } 7000, AEI 6B and AEI 801, Zeiss 109, and a Zeiss 10CR and an old Zeiss 9 I } believe. In my 18 years of experience, I've never seen peppering as a } result of a microscope contamination. It doesn't mean that it can't } happen, } just that in my experience, I haven't seen it. I can only talk about what } I've seen, or haven't seen. } } } PCan someone please explain why the solutions still concentrate on the } stain } and its condition rather than taking the tack that the problem lies with } the } Philips scope? } } Fred Monson } } } ---------- } } From: Monson, Frederick C. } } Sent: Wednesday, July 10, 2002 11:28 AM } } To: 'Stefan Geimer' } } Cc: 'List-Microscopy' } } Subject: RE: TEM, Epon sections, problem with "pepper" } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } But do you have the problem with a section that is taken from the } Philips } } to } } the Zeiss? } } } } Thanks, } } } } Fred Monson } } } } } ---------- } } } From: Stefan Geimer } } } Sent: Tuesday, July 9, 2002 2:04 PM } } } To: Microscopy-at-sparc5.microscopy.com } } } Subject: TEM, Epon sections, problem with "pepper" } } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } -----------------------------------------------------------------------. } } } } } } } } } I have the following problem: } } } I am observing Epon sections, stained with uranyl acetate and lead } } } citrate in an Philips EM 201. The sections show a really severe } } } contamination with electron dense grains. These grains are always } } } associated with embedded structures (membranes, microtubules etc.).The } } } problem started after I changed the cathode. The contamination looks } } } kinda like lead-stain granularity and I think it has something to do } } } with the lead. A section stained with uranyl acetate only looks fine } } } (but I need the lead to get enough stain). } } } The crazy thing is that I don´t have the problem when I use our other } } } scope, a Zeiss EM 10. So if I take one of my sections and have a look } at } } } it in the Zeiss EM 10, everything is perfect. The same section } observed } } } in the Philips EM 201 shows this contamination with electron dense } } } grains. Both scopes operated at comparable conditions (80 kv, cold } trap } } } etc.). So the contamination seems to be lead citrate and scope } } } dependend. } } } Anybody ever had that problem and might have an idea how to solve it? } } } } } } Stefan } } } } } } } } } } } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } } Stefan Geimer } } } MCDB Dept. } } } Yale University } } } P.O. Box 208103 } } } New Haven, CT 06520-8103 } } } U.S.A. } } } } } } Tel.: 203/432-3473 } } } Fax.: 203/432-6210 } } } } } } e-mail: stefan.geimer-at-yale.edu } } } °°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°° } } } } } } } } } } } } } } } } } } }
Assuming you really do mean nano particles and not micron sized then just drop them onto a carbon support filmed Cu grid and they will usually stick. Larger particles will tend to come off or charge and fly off but most nano and 10s of nanometre particles will be OK. Of course if they are magnetic the field might strip them off and if they are non conductive charging will be worse etc.
The alternative is to suspend them (in ethanol) and drop a drop of it onto the carbon support grid resting on a filter paper. They will also usually stick but beware of contaminating the surface by using dirty pipette, alcohol etc. Nothing should be stored in plastic and wash the utensils before use then you should be OK.
Good luck, Ron
On Fri, 12 Jul 2002 11:20:10 -0400 Barbara Maloney {bmalon01-at-fiu.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Group - what would be the best procedure to prep a copper TEM grid } with nanoparticles? What glue or adhesive would you suggest? I'm } actually using a STEM holder (to get TEM image) on a SEM. } Thanks } Barb } }
---------------------- Mr. R.C. Doole Department of Materials, University of Oxford. Parks Road, Oxford. OX1 3PH. UK. Phone +44 (0) 1865 273701 Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
Many thanks to all who replied to my question about removing cell membranes for SEM! Lots of leads, references, and a couple complete protocols to keep me busy for awhile.
What a great list!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
primary application is imaging of Pt coated (2-3nm) resist lines of 100nm and less. standard scope parameters under 100,000x have been 10kv spot2. now find that i must run at 20-30kv to image clearly, and image degrades over time (as little as ½ hour). FEI boosted µA to 334 and performed alignments. what amazes me is that i am no longer damaging my samples. its as if the beam lacks the "oomph" to bend over the lines like it used to at even 10kv. is this a "beam density" issue?
Mark Riggs SEM Metrology ASML, Wilton, CT mark.riggs-at-asml.com ph:203-761-6856 lab:203-761-4403
We have a TMC MICRO-g Vibration Isolation System Model # 65-17171-01 that we wish to sell. The platform is 86" wide by 71" deep with a seating cut out that is -at- 30" wide narrowing down to 12" and is about 38" deep. System has 4 isolation feet. With the feet, it requires an area that is 97" x 82". The weight of unit is between 2200 - 2500 lbs. Asking $4,000.00 or best offer plus shipping and handling charges. Interested parties should contact
Joe Goodhouse Confocal / EM Core Laboratory Manager Department of Molecular Biology Princeton University 609-258-5432 jgoodhouse-at-molbio.princeton.edu
Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
I have a question: With TEM how can I be sure that the feature I am looking at is/are dislocation(s)? Can you suggest any reading on dislocation imaging..Thanks
Aman Haque
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Aman Haque Dept of Mechanical & Industrial Engg University of Illinois at Urbana Champaign
Dopes anyone have a FEI Quanta working that s/he would talk to me about on the QT?
Sure would appreciate the info.
Thanks all and have a nice weekend,
Fred
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center West Chester University South Church Street and Rosedale West Chester, Pennsylvania, USA, 19383 Phone: 610-738-0437 FAX: 610-738-0437 fmonson-at-wcupa.edu CASI URL: http://darwin.wcupa.edu/casi/ WCUPA URL: http://www.wcupa.edu/ Visitors URL: http://www.wcupa.edu/_visitors/
Dislocations have very specific visibility criteria in a TEM, which are basically determined by their burgers vector. I am not close to my TEM books right now, so I can't be more specific, but I am sure there are other people here who are very familiar with dislocations. To read more about dislocations and TEM, I would suggest to take a look at Sir Peter Hirsch's book . I think, it's called "Electron Microscopy of Thin Crystals".
mike
} } } } } } } } } } WE HAVE MOVED { { { { { { { { { please make a note of the new address below
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: mdhaque [mailto:mdhaque-at-students.uiuc.edu] Sent: Friday, July 12, 2002 1:13 PM To: Microscopy-at-sparc5.microscopy.com
Hi,
I have a question: With TEM how can I be sure that the feature I am looking at is/are dislocation(s)? Can you suggest any reading on dislocation imaging..Thanks
Aman Haque
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Aman Haque Dept of Mechanical & Industrial Engg University of Illinois at Urbana Champaign
Yes, Steve, the much higher absorption coefficient for FE prevents the very soft Boron X-rays from being emitted from FeB2 in sufficient quantity to be detected.
Ron Vane XEI Scientific
-----Original Message----- } From: Steven Celotto {s.celotto-at-liverpool.ac.uk} Cc: Microscopy Society of America {Microscopy-at-sparc5.microscopy.com}
Another book that you might want to look at is Williams and Carter's book, Transmission Electron Microscopy. It is available from Plenum.
One slick method to determine the Burgers vector of a dislocation is to set up a multi-beam convergent beam diffraction pattern (e.g. three beams) with the beams at or very nearly at the exact Bragg condition and place the spot over the dislocation. You also have to have dynamical diffraction conditions so that you can see the HOLZ lines in the disks. If you don't see the HOLZ lines, then lower the voltage of the microscope. You will see that there are nodes in the HOLZ lines that appear to split the lines. If you know the g for a HOLZ line, the number of nodes in the line tells you the value of g dot b for that g (e.g. g * b=1,2,3,etc.). With three such disks, you have three equations and three unknowns and you can determine b from one pattern without the need to tilt the sample to other orientations to find g dot b = 0 conditions where the dislocation will disappear. See Spence and Zuo's book, Electron Microdiffraction, also available from Plenum.
If you use the tilting to find two-beam conditions where the dislocation disappears, two such g dot b =0 conditions, where the g's are not collinear, will give you b because it is mutually perpendicular to both g's.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center P. O. Box 11472 (letters) Guys Run Rd. (packages) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8515 (fax)
-----Original Message----- } From: Mike Bode [mailto:mb-at-Soft-Imaging.com] Sent: Friday, July 12, 2002 7:18 PM To: 'Microscopy-at-MSA.Microscopy.Com'
Dislocations have very specific visibility criteria in a TEM, which are basically determined by their burgers vector. I am not close to my TEM books right now, so I can't be more specific, but I am sure there are other people here who are very familiar with dislocations. To read more about dislocations and TEM, I would suggest to take a look at Sir Peter Hirsch's book . I think, it's called "Electron Microscopy of Thin Crystals".
mike
} } } } } } } } } } WE HAVE MOVED { { { { { { { { { please make a note of the new address below
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: mdhaque [mailto:mdhaque-at-students.uiuc.edu] Sent: Friday, July 12, 2002 1:13 PM To: Microscopy-at-sparc5.microscopy.com
Hi,
I have a question: With TEM how can I be sure that the feature I am looking at is/are dislocation(s)? Can you suggest any reading on dislocation imaging..Thanks
Aman Haque
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Aman Haque Dept of Mechanical & Industrial Engg University of Illinois at Urbana Champaign
.. And let's not forget Hull and Bacon, "Introduction to Dislocations" from Pergamon Press. And I think, there's also information in L. Reimer, "Transmission Electron Microscopy" from Springer.
mike
} } } } } } } } } } WE HAVE MOVED { { { { { { { { { please make a note of the new address below
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Walck, Scott D. [mailto:walck-at-ppg.com] Sent: Friday, July 12, 2002 7:25 PM To: Microscopy (E-mail) Cc: 'Mike Bode'
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
The following news item was in the July 13-14, 2002 International Herald Tribune, sandwiched between articles about Enron and Worldcom:
SEMICONDUCTOR BUY: Veeco Instruments, Inc. said it would purchase FEI Co., a maker of gear for the semiconductor industry, for $1 billion in stock in a deal that would make it the sixth-largest U. S. maker of chip-manufacturing equipment.
The acquisition is the latest in a string of acquisitions by Veeco, a maker of precision instruments and electronic testing products, and signals further consolidation in the semiconductor equipment market.
The purchase will give Veeco a larger presence in the business of microscopic measurement systems used to make semiconductors, data storage devices and other electronic products.
The Amsterdam-based Philips Electronics NV, the largest holder of FEI shares with a 25% stake, will own about 15% of the combined company, a spokesman said.
Reuters, Bloomberg
I thought at least some members of this listserver would find this information quite interesting. We might also pause and ask whether this kind of consolidation is positive, negative, or neutral for the future our industry and market place.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
Sorry to post this but we have been having troubles posting and it may be fixed now. So I am sending this as my staff have nothing actually to post at the moment.
~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~- Darryn Capes-Davis BE IT Manager Children's Medical Research Institute 214 Hawkesbury Road Westmead, NSW 2145 Australia Tel. +61 2 9687 2800 Fax +61 2 9687 2120 Email dcapes-davis-at-cmri.usyd.edu.au ~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-~-
The exhaustive treatment of the "classical", two beam diffraction contrast of dislocations in TEM is contained in the book of A.K.Head, P.Humble, L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron micrographs and defect identification". On the ground of the minimum theoretical knowledge that any electron microscopist has to hold, it is presented in all details, computer codes in Fortran included, the method that can generate by computation the "classical" contrast of a rectilinear dislocation and a complex of two dislocations and three stacking faults. There are in use such computer programs, commercial and free ones, that either are applying the codes given in that book or are providing more advanced treatment dealing with the same problem. It is surprising to see that the old "classical" knowledge of the diffraction contrast interpretation has faded away during the HRTEM offensive and that the necessity of looking back to the "simple" two beam diffraction contrast is more and more a request.
Corneliu Sarbu, PhD Department of Metallurgy and Applied Materials Science (MTM Dept.) Catholic University of Leuven (KULeuven) Kasteelpark ARENBERG nr. 44 B-3001 Heverlee-Leuven, Belgium **************************************************************** Phone: +32-16-32.1241 - office +32-16-32.1264 - secretary of department Fax: +32-16-32.1992 or +32-16-32.1270 e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be ****************************************************************
At 14:13 12/07/02 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I suspect history will repeat itself yet again. The two other EM manufacturers that come to mind that sold because of their semiconductor production products are ETEC and Amray.
ETEC's SEM business quietly folded twenty years ago, just a couple of years after being sold to Perkin-Elmer primarily because of their electron beam lithography products. ETEC was the original American SEM manufacturer, had major market share here for years and put out a fine instrument. But, in the end, sold out entirely to a manufacturer who wanted to augment their optical stepper line of lithography equipment with the electron beam mask makers from ETEC.
Amray's history in this regard is probably recent enough that most here have at least heard some of it. While they have not pulled completely out of the general EM business, what steps they have taken would seem to point to their long term commitment to.
In both cases, customers were hurt. Not just by the loss of the manufacturers, but also by the way their EM businesses were casually tossed aside. Perhaps, this time, someone will at least make an effort to make it painless for their current customers. I hesitate to think that anyone would actually consider making an effort to maintain and improve their general EM business, or spin it off as a separate division or company.
Given FEI's broader base of EM instruments, perhaps it will happen. I wouldn't bet on it though.
There, a gauntlet thrown down to anyone from Veeco who may be reading this. Please prove me wrong and keep this business going. You have a lot of very loyal customers out there.
As far as whether such change is good, bad or indifferent, I think it's just natural. Over the last few decades we've seen EM emerge from the research labs to applications that intersect virtually every industry. The growth of electron beam instruments has branched in many, often overlaying, directions. This provides opportunities for some companies that can pio neer new applications - applications that can often prove more profitable.
The good news is that EM has become such a pervasive and useful set of technologies. Add to that the relatively high profit margin for manufacturer's (particularly those just starting out), and you have a formula for a constantly competitive environment. The more the industry tries to consolidate, I believe, the more new manufacturer's we'll see. The unfortunate thing is trying to keep up with all the name changes.
(A little disclaimer might be in order here. As many of you know, I am a rather outspoken third-party service provider for EM and other instruments. I never particularly liked the corporate environment of Philips in general, but they have apparently done a very good job of keeping their customers satisfied. In over twenty years of business, I have yet to work on one of their instruments, and that's very rare. They've done a good job, and I hate to see the good ones go.)
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174
On Saturday, July 13, 2002 11:03 PM, Garber, Charles A. [SMTP:cgarber-at-2spi.com] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } The following news item was in the July 13-14, 2002 International Herald } Tribune, sandwiched between articles about Enron and Worldcom: } } SEMICONDUCTOR BUY: } Veeco Instruments, Inc. said it would purchase FEI Co., a maker of gear for } the semiconductor industry, for $1 billion in stock in a deal that would } make it the sixth-largest U. S. maker of chip-manufacturing equipment. } } The acquisition is the latest in a string of acquisitions by Veeco, a maker } of precision instruments and electronic testing products, and signals } further consolidation in the semiconductor equipment market. } } The purchase will give Veeco a larger presence in the business of } microscopic measurement systems used to make semiconductors, data storage } devices and other electronic products. } } The Amsterdam-based Philips Electronics NV, the largest holder of FEI shares } with a 25% stake, will own about 15% of the combined company, a spokesman } said. } } Reuters, Bloomberg } } } I thought at least some members of this listserver would find this } information quite interesting. We might also pause and ask whether this } kind of consolidation is positive, negative, or neutral for the future our } industry and market place. } } Chuck } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President 1-800-2424-SPI } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.2spi.com } ######################## } ============================================ } } } }
Amray is still alive and kicking. Even that they are part of KLA-Tencor. The difference with them is that they no longer support thermionic systems--just 305FE Schottky gun systems. Since this is the same gun used in the KLA CD-SEM systems, my hope is that SEM support will continue for some time. I believe that KLA made a major strategic blunder by exiting the general purpose SEM market in favor of the high cost CD-SEMs. These giant systems are all over the place at auctions and bone yards now as the semi market has taken a nose dive. On the other hand I don't see many used SEMs of any type come up for sale at the rate of CD-SEMs. If KLA had kept the service going for the non-FESEMs, they would have had a nice cash flow instead of a loss.
They do not make lab SEMs any more. But the do service the existing FESEMs. That is better than nothing. Mine is very reliable and rarely needs service. Mostly, it is cleaning and aperture replacement, and holder cleaning. I've heard that all of the SEM technology is moving from MA to CA. Perhaps parts supply will stay in MA. I'm not sure what the motivation of this action is, but hopefully it will work out.
I'm working on a new XL-30 Sirion and am impressed by how nicely it is built. But for me, the Amray is much easier to use. Fast and efficient. Nice balance of computer control and user interface. Probably just a personal preference.
Let's see what happens. You can bet that if the manufacturers suffer economically, we users will too.
gary g.
At 01:56 PM 7/14/2002, you wrote:
} I suspect history will repeat itself yet again. The two other EM } manufacturers that come to mind that sold because of their semiconductor } production products are ETEC and Amray. } } ETEC's SEM business quietly folded twenty years ago, just a couple of years } after being sold to Perkin-Elmer primarily because of their electron beam } lithography products. ETEC was the original American SEM manufacturer, had } major market share here for years and put out a fine instrument. But, in } the end, sold out entirely to a manufacturer who wanted to augment their } optical stepper line of lithography equipment with the electron beam mask } makers from ETEC. } } Amray's history in this regard is probably recent enough that most here } have at least heard some of it. While they have not pulled completely out } of the general EM business, what steps they have taken would seem to point } to their long term commitment to. } } In both cases, customers were hurt. Not just by the loss of the } manufacturers, but also by the way their EM businesses were casually tossed } aside. Perhaps, this time, someone will at least make an effort to make it } painless for their current customers. I hesitate to think that anyone } would actually consider making an effort to maintain and improve their } general EM business, or spin it off as a separate division or company. } } Given FEI's broader base of EM instruments, perhaps it will happen. I } wouldn't bet on it though. } } There, a gauntlet thrown down to anyone from Veeco who may be reading this. } Please prove me wrong and keep this business going. You have a lot of } very loyal customers out there. } } As far as whether such change is good, bad or indifferent, I think it's } just natural. Over the last few decades we've seen EM emerge from the } research labs to applications that intersect virtually every industry. The } growth of electron beam instruments has branched in many, often overlaying, } directions. This provides opportunities for some companies that can pio } neer new applications - applications that can often prove more profitable. } } The good news is that EM has become such a pervasive and useful set of } technologies. Add to that the relatively high profit margin for } manufacturer's (particularly those just starting out), and you have a } formula for a constantly competitive environment. The more the industry } tries to consolidate, I believe, the more new manufacturer's we'll see. } The unfortunate thing is trying to keep up with all the name changes. } } (A little disclaimer might be in order here. As many of you know, I am a } rather outspoken third-party service provider for EM and other instruments. } I never particularly liked the corporate environment of Philips in } general, but they have apparently done a very good job of keeping their } customers satisfied. In over twenty years of business, I have yet to work } on one of their instruments, and that's very rare. They've done a good } job, and I hate to see the good ones go.) } } } Allen R. Sampson } Advanced Research Systems } 317 North 4th. Street } St. Charles, Illinois 60174 } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com } } } On Saturday, July 13, 2002 11:03 PM, Garber, Charles A. } [SMTP:cgarber-at-2spi.com] wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } } } The following news item was in the July 13-14, 2002 International Herald } } Tribune, sandwiched between articles about Enron and Worldcom: } } } } SEMICONDUCTOR BUY: } } Veeco Instruments, Inc. said it would purchase FEI Co., a maker of gear } for } } the semiconductor industry, for $1 billion in stock in a deal that would } } make it the sixth-largest U. S. maker of chip-manufacturing equipment. } } } } The acquisition is the latest in a string of acquisitions by Veeco, a } maker } } of precision instruments and electronic testing products, and signals } } further consolidation in the semiconductor equipment market. } } } } The purchase will give Veeco a larger presence in the business of } } microscopic measurement systems used to make semiconductors, data storage } } devices and other electronic products. } } } } The Amsterdam-based Philips Electronics NV, the largest holder of FEI } shares } } with a 25% stake, will own about 15% of the combined company, a spokesman } } said. } } } } Reuters, Bloomberg } } } } } } I thought at least some members of this listserver would find this } } information quite interesting. We might also pause and ask whether this } } kind of consolidation is positive, negative, or neutral for the future } our } } industry and market place. } } } } Chuck } } } } ============================================ } } } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } } President 1-800-2424-SPI } } SPI SUPPLIES FAX: 1-610-436-5755 } } PO BOX 656 e-mail:cgarber-at-2spi.com } } West Chester, PA 19381-0656 USA } } Cust.Service: spi2spi-at-2spi.com } } } } Look for us! } } ######################## } } WWW: http://www.2spi.com } } ######################## } } ============================================ } } } } } } } }
One of our EM users is likely to need a heart pacemaker soon. Just to make sure, we'd like to ask if there are any known problems associated with pacemakers operating in the vicinity of TEMs and SEMs?
There are several variants in pacemakers. I worked on them back when in the early models, fixed rate type [RCA/Cordis]. However, I would suggest to the EM/TEM user that he consult the mfg. on the specific type he has and its' precautions. If I'm not mistaken some have radio transmitters embedded for remote monitoring purposes. EMP [Electromagnetic Pulse] is an issue but I assume you aren't working on weapons systems or susceptibility of weapons systems. From an operators perspective, just turning knobs and focusing, I can't think of a reason why operating either instrument should be a problem.
Peter Tomic Anadigics, Inc.
-----Original Message----- } From: Sally Stowe [mailto:stowe-at-rsbs.anu.edu.au] Sent: Sunday, July 14, 2002 6:34 PM To: microscopy-at-sparc5.microscopy.com
One of our EM users is likely to need a heart pacemaker soon. Just to make sure, we'd like to ask if there are any known problems associated with pacemakers operating in the vicinity of TEMs and SEMs?
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We have a TMC MICRO-g Vibration Isolation System Model # 65-17171-01 that we wish to sell. The platform is 86" wide by 71" deep with a seating cut out that is -at- 30" wide narrowing down to 12" and is about 38" deep. System has 4 isolation feet. With the feet, it requires an area that is 97" x 82". The weight of unit is between 2200 - 2500 lbs. Asking $4,000.00 or best offer plus shipping and handling charges. Interested parties should contact
Joe Goodhouse Confocal / EM Core Laboratory Manager Department of Molecular Biology Princeton University 609-258-5432 jgoodhouse-at-molbio.princeton.edu
Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
} } } Yes, Steve, the much higher absorption coefficient for FE } prevents the very } soft Boron X-rays from being emitted from FeB2 in sufficient } quantity to be } detected. } } Ron Vane } XEI Scientific } } -----Original Message----- } } From: Steven Celotto {s.celotto-at-liverpool.ac.uk} } Cc: Microscopy Society of America {Microscopy-at-sparc5.microscopy.com} } Date: Wednesday, July 10, 2002 10:21 PM } Subject: Re: Carbon Quantitation by EDX } } } } ------------------------------------------------------------- } ----------- } } The Microscopy ListServer -- Sponsor: The Microscopy } Society of America } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------- } ----------. } } } } } } I am curious to hear the answer to this question from } Jacques and I have } } something to add. } } Once I performed EDS analyses on a sample of BC particles on } C film in } order to } } test a new EDS analysis system installed on a new FEG 200KeV } TEM. After } selecting } } the correct time constant in the EDS software I could get nice } distinguishable C } } and B peaks. Later I tried doing a similar EDS analysis on large FeB2 } particles in } } } } a Fe matrix. I mention large here to stress that the } particles went through } the } } specimen foil so most of the X-rays were being emitted } directly from the } particles } } } } without passing through the Fe matrix before reaching the } detector. I never } saw } } even a hint of a B peak. These ppts should have contained 33at%B. } } Was I doing something wrong or is that an indication of how } easily boron } X-rays } } are absorbed within a sample containing large z atoms? } } } } Faerber Jacques wrote: } } } } } } -------------------------------------------------------------- } ---------- } } } The Microscopy ListServer -- Sponsor: The Microscopy } Society of America } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } -------------------------------------------------------------- } ---------. } } } } } } Hi all } } } } } } In the same idee, what about mesurements on boron carbide } ? Carbon AND } } } boron concentrations ! I see nice spectras, without } anything else ( a bit } } } O, some times Al and N). And I have variations between } samples in the B/C } } } ratio. In such a case can I mesure only ratio variation, or is it } possible } } } to try some quantification (with standards). The sample is bulk B4C } } } and laser ablation thick layers (with dropplets). Primary } energy 3 to 5 } } } keV. } } } } } } I think it's a bit pretentious to quantify. What is } other's opinion ? } } } } } } J. Faerber } } } IPCMS-GSI } } } (Institut de Physique et Chimie des Matériaux de Strasbourg } } } Groupe Surface et Interfaces) } } } 23, rue de Loess } } } 67037 Strasbourg CEDEX } } } France } } } } } } Tel 00 33(0)3 88 10 71 01 } } } Fax 00 33(0)0 88 10 72 48 } } } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } } } On Tue, 9 Jul 2002, Ronald Vane wrote: } } } } } } } } } -------------------------------------------------------------- } ---------- } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } -------------------------------------------------------------- } ---------. } } } } } } } } } } } } Dear Chris and all: } } } } } } } } Quantitative analysis of Carbon by EDX is nearly } impossible because of } the } } } } very shallow penetration depth of the Carbon X-rays. You } really just } measure } } } } the surface. Surface analysis techniques such as XPS and } Auger also } show } } } } that thin carbon films love to form on surfaces. If you } have an XPS you } use } } } } your ion gun to sputter off the carbon surface scum to } see the real } surface. } } } } } } } } Dry ashing in a plasma cleaner can also remove the carbon surface } layer. } } } } Sputter etching can be used to remove gold and silver coatings. } } } } } } } } Ron Vane } } } } XEI Scientific } } } } 3124 Wessex Way, Redwood City, CA 94061 } } } } 650-369-0133 } } } } www.SEMCLEAN.com } } } } } } } } Note: XEI Scientific makes the EVACTRON plasma cleaning } system for } Electron } } } } Microscope Chambers and FIBs, but does not make desk top } plasma dry } ashers } } } } or sputter etchers. } } } } } } } } -----Original Message----- } } } } } From: Chris Peppiatt {chris.peppiatt-at-nuigalway.ie} } } } } To: Microscopy-at-sparc5.microscopy.com } {Microscopy-at-sparc5.microscopy.com} } } } } Date: Monday, July 08, 2002 3:22 PM } } } } Subject: Carbon Quantitation by EDX } } } } } } } } } } } } } } ------------------------------------------------------------- } ----------- } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } } } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } } } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ------------------------------------------------------------- } ----------. } } } } } } } } } } } } } } } Dear All, } } } } } } } } } } Someone has asked me to do some work for them on } mineral specimens. } The } } } } } samples will be mounted in resin and polished and we } will then coat } with } } } } } carbon. Obviously we will set our software to } deconvolute carbon from } the } } } } } analyses. We have the option to coat samples with either gold or } silver and } } } } } then look at the carbon content. I suppose the } questions I would like } to } } } } } have answered are: } } } } } } } } } } (1) Heavy elements like gold or silver will absorb some } of the light } } } } } element (inc. carbon) X-rays when used as coatings. Is } there any way } of } } } } } correcting for this to get an accurate quantitative } analysis of carbon } } } } content? } } } } } } } } } } (2) Is there any way of removing either gold/silver or } carbon coatings } from } } } } } such samples except for the obvious method of } regrinding/polishing the } } } } } coating off? } } } } } } } } } } Thanks in advance. } } } } } } } } } } Regards, } } } } } } } } } } Chris Peppiatt } } } } } } } } } } } } } } } ============================================ } } } } } Dr. Chris Peppiatt (Experimental Officer), } } } } } The National Centre for Biomedical Engineering Science, } } } } } Science & Engineering Technology Building, } } } } } National University of Ireland Galway, } } } } } Galway City, Co. Galway, } } } } } Republic of Ireland. } } } } } chris.peppiatt-at-nuigalway.ie } } } } } Phone: +353 91 512157 Fax: +353 91 750596 } } } } } ============================================= } } } } } } } } } } } } } } } } } } } } } } } } } } } -- } } Dr. Steven Celotto } } Department of Engineering } } Materials Science & Engineering } } University of Liverpool } } Brownlow Hill } } Liverpool L69 3BX } } UNITED KINGDOM } } } } Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692 } } Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675 } } email: s.celotto-at-liv.ac.uk } } } } } } } } } } }
Your posting reminded me of the software available at the MSA/MAS library. There is a public domain software program that is called "oneDis" (I think) that will simulate the image of a dislocation after providing the program with the imaging parameters. I would recommend that you donwload the software and "play" with it to learn what dislocations look like at different imaging conditions. It is quite useful and it will help with the original question that was posted.
You can get to the EMMPDL library from the MSA website. Here are instructions that I copied
EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National Laboratory. The following FTP Site give you access to these Software Libraries. Access is by anonymous Login:
Host : WWW.AMC.ANL.GOV or the mirror site WWW.MSA.Microscopy.Com
Login: Username = Anonymous Password = Your Email Address
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center P. O. Box 11472 (letters) Guys Run Rd. (packages) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8515 (fax)
-----Original Message----- } From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be] Sent: Sunday, July 14, 2002 1:31 PM To: Microscopy-at-sparc5.microscopy.com
The exhaustive treatment of the "classical", two beam diffraction contrast of dislocations in TEM is contained in the book of A.K.Head, P.Humble, L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron micrographs and defect identification". On the ground of the minimum theoretical knowledge that any electron microscopist has to hold, it is presented in all details, computer codes in Fortran included, the method that can generate by computation the "classical" contrast of a rectilinear dislocation and a complex of two dislocations and three stacking faults. There are in use such computer programs, commercial and free ones, that either are applying the codes given in that book or are providing more advanced treatment dealing with the same problem. It is surprising to see that the old "classical" knowledge of the diffraction contrast interpretation has faded away during the HRTEM offensive and that the necessity of looking back to the "simple" two beam diffraction contrast is more and more a request.
Corneliu Sarbu, PhD Department of Metallurgy and Applied Materials Science (MTM Dept.) Catholic University of Leuven (KULeuven) Kasteelpark ARENBERG nr. 44 B-3001 Heverlee-Leuven, Belgium **************************************************************** Phone: +32-16-32.1241 - office +32-16-32.1264 - secretary of department Fax: +32-16-32.1992 or +32-16-32.1270 e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be ****************************************************************
At 14:13 12/07/02 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Title: Optical Microscopy and Imaging in the Biomedical Sciences
When: October 9 - October 18, 2002
Where: Marine Biology Laboratory, Woods Hole, MA, USA
Tuition: $2200 (Includes room and board, text, handouts, supplies)
Application Deadline: July 25, 2002
Admission application and information: Carol Hamel, Admissions Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543-1015 (508) 289-7401 Internet: admissions-at-mbl.edu WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)
Course Director: Colin S. Izzard, State University of New York -at- Albany Phone: [518] 442 - 4367 EMail: csizzard-at-csc.albany.edu
Course Description:
For Whom: Designed primarily for research scientists, physicians, postdoctoral trainees and advanced graduate students in animal, plant, medical and material sciences. Non-biologists seeking a comprehensive introduction to microscopy and video-imaging will benefit greatly from this course as well. There are no specific prerequisites, but an understanding of the basic principles of optics is desirable. Limited to 24 students.
The eight day course consists of lectures, laboratory demonstrations, exercises and discussions that will enable the participant to obtain and interpret microscope images of high quality, to perform quantitative optical
measurements, and to produce photographic and video records for documentation and analysis.
Topics to be covered include: principles of microscope design and image formation bright field, dark field, phase contrast, polarized light, differential interference contrast, interference reflection, and fluorescence microscopy confocal scanning microscopy, multiphoton excitation fluorescence microscopy and image deconvolution digital image restoration and 3-D reconstruction video imaging, recording, enhancement, and intensification analog and digital image processing and analysis fluorescent probes and ratiometric-imaging laser tweezers and laser scissors
Applications to live cells will be emphasized; other specimens will be covered as well.
Students will have direct hands-on experience with state-of-the-art microscopes, video and digital cameras, recorders and image processing equipment provided by major optical and electronics companies. Instruction will be provided by experienced staff from universities and industry.
STUDENTS ARE ENCOURAGED TO BRING THEIR OWN BIOLOGICAL (PRIMARY CULTURES, CELL LINES, PREPARED SLIDES, ETC.) AND MATERIAL SPECIMENS AND TO USE THEM FOR COURSE EXERCISES, WHERE APPROPRIATE. Cell culture facilities are available. Students are highly encouraged to discuss individual research problems with the academic and commercial faculty.
For faculty list and additional information, see: http://www.mbl.edu
} Hmm--- strictly, any } processing *at all* corrupts the primary image data.
Looking at something corrupts it's nature and asking a question about it eliminates other perpectives. Cats, electrons and trees falling in the forest are all phenomena that illustrate the point that detection of absolute truth is inherently flawed.
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
Then by all means, let us stop looking and asking questions lest we corrupt truth...
----- Original Message ----- } From: Michael Cammer {cammer-at-aecom.yu.edu}
Greetings all,
I routinely have to look at semiconductor devices from top down for failure analysis. All has been fine with our images until we started imaging with our new microscope.
To give some background, what we are looking at is aluminum metal lines with tungsten interconnects standing proud of the aluminum. When we image what we see is significant darkening of the image around groups of interconnects and, in extreme cases, around individual interconnects.
We see this primarily when imaging in backscatter, but sometimes also using In-lens and E-T secondary detectors. does anybody have a reasonable explanation to what this phenomenon is?
Unsubscribe jfmjfm-at-umich.edu -- John Mansfield PhD MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42! 16' 48" Long. 83! 43' 48"
Currently we are looking for a microtome machine for our lab. Till now, we use a microtome machine in another lab which is SORVAU & CAT 15350. It has light source of 15400. We also like to have same type of microtome with reasonable price.
So, any of you have any idea about the microtome, please let us know. We will appreaciate that very much.
The Texas Society for Microscopy (http://www.texasmicroscopy.org/) will have its Fall Meeting on Oct. 24-26, 2002 in Austin, TX at the Embassy Suites Austin North Hotel .
The Thursday afternoon workshop will be on "TEM materials preparation tools", presented by Dr. Rocco Cerchiara, Applications Laboratory Manager, E. A. Fischione.
The Friday afternoon workshop will be "Cryo-microtomy, with emphasis on TEM sample preparation", presented by Dr. Gregory Becker, Product Manager, RMC Products by Boeckeler.
Workshops sponsored by ASI, Inc. and TSM
There is also a call for papers for this meeting. All technologies and disciplines involving microscopy in any form are invited to contribute.
Please see our website, http://www.texasmicroscopy.org/ , for details, registration, additional information, and author's instructions
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-598-1291 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
It sounds as though you're getting a shadowing effect from the interconnects. If a significant fraction of the backscattered electrons otherwise received by your detector is blocked by the interconnects, then this will show up as a darkening around these 'taller' features. This would be more pronounced for W shadowing Al, as the BSEs coming off Al would tend to be comparatively low energy, and thus more easily blocked by the W. Assuming the geometry of the objects being imaged is the same as in your previous 'scope, then it sounds as though either: 1) your collection geometry for the new detector arrangement is larger (giving a larger solid angle of collection in which some part of the signal is blocked), or 2) your new detector has a different energy sensitivity as compared to the 'old' detector (less sensitivity to lower energy electrons, OR a lower detection threshold energy), or possibly even 3) the magnetic field strength in your chamber is high enough to cause electrons emitted from your sample to spiral up about the field lines to your detector, giving the same effect as if you had a larger solid angle of collection. This last sounds like a possibility because of your mention of an in-lens detector, which would tend to need a pretty strong Z-axis magnetic field in order to operate.
Cheers,
Ben Simkin (simkin-at-egr.msu.edu)
} Greetings all, } } I routinely have to look at semiconductor devices from top down for } failure analysis. All has been fine with our images until we started } imaging with our new microscope. } } To give some background, what we are looking at is aluminum metal lines with } tungsten interconnects standing proud of the aluminum. When we image what we } see is significant darkening of the image around groups of interconnects } and, in extreme cases, around individual interconnects. } } We see this primarily when imaging in backscatter, but sometimes also using } In-lens and E-T secondary detectors. does anybody have a reasonable } explanation to what this phenomenon is? } } Thanks for you time } } Nick
Hum...what is the new scope? What is the old scope?
What mag and KV are you using? Are the specimens coated? With what and how much? What feature sizes are you dealing with? Underneath the Al interconnects should be TiW barrier metal if that process required it. Z contrast will be high. If you are shooting with high KV, large spot size and large aperture, I'd suspect charging. Make sure that the specimen is grounded and is coated. And use low KV (2-5) and a small probe diameter.
Posting a couple of sample images to your web site would be helpful.
gary g.
At 01:12 PM 7/15/2002, you wrote:
} Greetings all, } } I routinely have to look at semiconductor devices from top down for } failure analysis. All has been fine with our images until we started } imaging with our new microscope. } } To give some background, what we are looking at is aluminum metal lines with } tungsten interconnects standing proud of the aluminum. When we image what we } see is significant darkening of the image around groups of interconnects } and, in extreme cases, around individual interconnects. } } We see this primarily when imaging in backscatter, but sometimes also using } In-lens and E-T secondary detectors. does anybody have a reasonable } explanation to what this phenomenon is? } } Thanks for you time } } Nick
Hello, I am looking for a Split Data Rotation unit (Part PW6762) for a Philips 515 SEM. If you have such a device or know where I can get one I would appreciate hearing from you.
ourselves (e.g. personal biases, the fact that we haven't considered all possible explanations of the results etc.)
An honest and realistic scientist realises that he/she throws up as many questions as he/she answers. Maybe in science, we just hope for better and better approximations to the truth...
Of course, all this assumes that there is such an entity as "the truth", independent of us, our thoughts, and our observations. Many philosophers would deny that. I see no way that we can do science without this assumption and we have to part company with some current philisophy as a result. This assumption is of course deeply embedded in western culture and very much part of the Judaeo-Christian and Muslim traditions.
----- Original Message ----- } From: {"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com} To: "Michael Cammer" {cammer-at-aecom.yu.edu} Cc: {microscopy-at-sparc5.microscopy.com} Sent: Monday, July 15, 2002 6:59 PM
Nicol;
What is the surface passivated with? If it is polyimide or bisbenzocyclobutene you may get a little "burning" and definitely charging, if uncoated. Also, hydrocarbons in your sample chamber will be, so to speak, "painted" on the surface and look like the region you just rastered on the SEM when you back out in mag.
A little more info. and someone may be able to help you with this.
Regards,
Peter Tomic Anadigics, inc.
-----Original Message----- } From: Nicol Aitken [mailto:nicol-at-semiconductor.com] Sent: Monday, July 15, 2002 4:12 PM To: microscopy-at-sparc5.microscopy.com
Greetings all,
I routinely have to look at semiconductor devices from top down for failure analysis. All has been fine with our images until we started imaging with our new microscope.
To give some background, what we are looking at is aluminum metal lines with tungsten interconnects standing proud of the aluminum. When we image what we see is significant darkening of the image around groups of interconnects and, in extreme cases, around individual interconnects.
We see this primarily when imaging in backscatter, but sometimes also using In-lens and E-T secondary detectors. does anybody have a reasonable explanation to what this phenomenon is?
Thank you Ian for a thoughtful and insightful contribution -- indeed, one that allows us to continue to believe in the usefulness of our endeavors to uncover various aspects of the truth that we hope is out there.
Pragmatically, we can believe that this world is below the shadow of a dream, and taught by time take it so -- excepting always steam.*
*Rudyard Kipling's "McAndrew's Hymn"
----- Original Message ----- } From: "Ian MacLaren" {maclaren-at-tu-darmstadt.de}
Michael Cammer wrote: } } Hmm--- strictly, any } } processing *at all* corrupts the primary image data. } } Looking at something corrupts it's nature and asking a question about it } eliminates other perpectives. Cats, electrons and trees falling in the } forest are all phenomena that illustrate the point that detection of } absolute truth is inherently flawed.
The last year we installed ENCA 200 system in our JSM 840 SEM. Last week when we repaired our SEM, liquid nitrogen in dewar was emptied to remove water. Then we re-filled new liquid nitrogen through "warm up" and "cool down" procedures. After that, we tried to calibrate this ISIS system. When making "discriminators only", a message showed "ISIS calibration unable to measure strobe peak. Abandoning calibration". At somebody's suggestion, several times of conditioner were conducted, but the situation is the same. We couldn't do anything now. The system seems to be locked. Also when running EDS, no any peaks could be found, showing very high deadtime (99%). Even without beam, the deadtime is also very high. It was suspected that the detector system was damaged. I suspect that the strobed zero peak is generated in the XP2 pulse processor, not in the detector. Why "discriminators only" didn't work?
If anybody of you has experience with this type of problems, I would like to have your opinions and thoughts. Thanks!
Our system No. 05426-2390-414-51, Model 6506, att 1: 32804c802, att2: 0120024, window: ATW2 det. area 10 mmxmm.
Yuquan Ding Materials Labs Dept. of Mechanical Engineering University of Waterloo Waterloo, ON N2L 3G1 519-888-4567 x3766 Fax: 519-888-6197 Email: yding-at-uwaterloo.ca
OneDis is the program developed by Head et al. It may be still in Fortran and it may still have its awkward input format (emulating the old punch card format). Prof. Veli-Tapani Kuokkala has repackaged the code into a user-friendly Windows format for PC. The program can still be download from his webpage.
http://www.tut.fi/units/ms/elm/enindex.htm
"Walck, Scott D." wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Your posting reminded me of the software available at the MSA/MAS library. There is a public domain software program that is called "oneDis" (I think) that will simulate the image of a dislocation after providing the program with the imaging parameters. I would recommend that you donwload the software and "play" with it to learn what dislocations look like at different imaging conditions. It is quite useful and it will help with the original question that was posted. } } You can get to the EMMPDL library from the MSA website. Here are instructions that I copied } } EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National Laboratory. The following FTP Site give you access to these Software Libraries. Access is by anonymous Login: } } Host : WWW.AMC.ANL.GOV or } the mirror site WWW.MSA.Microscopy.Com } } Login: } Username = Anonymous } Password = Your Email Address } } -Scott } } Scott D. Walck, Ph.D. } PPG Industries, Inc. } Glass Technology Center } P. O. Box 11472 (letters) } Guys Run Rd. (packages) } Pittsburgh, PA 15238-0472 } } Walck-at-PPG.com } } (412) 820-8651 (office) } (412) 820-8515 (fax) } } -----Original Message----- } } From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be] } Sent: Sunday, July 14, 2002 1:31 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: Re: TEM Dislocations } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } The exhaustive treatment of the "classical", two beam diffraction contrast } of dislocations in TEM is contained in the book of A.K.Head, P.Humble, } L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron } micrographs and defect identification". On the ground of the minimum } theoretical knowledge that any electron microscopist has to hold, it is } presented in all details, computer codes in Fortran included, the method } that can generate by computation the "classical" contrast of a rectilinear } dislocation and a complex of two dislocations and three stacking faults. } There are in use such computer programs, commercial and free ones, that } either are applying the codes given in that book or are providing more } advanced treatment dealing with the same problem. } It is surprising to see that the old "classical" knowledge of the } diffraction contrast interpretation has faded away during the HRTEM } offensive and that the necessity of looking back to the "simple" two beam } diffraction contrast is more and more a request. } } Corneliu Sarbu, PhD } Department of Metallurgy and Applied Materials Science (MTM Dept.) } Catholic University of Leuven (KULeuven) } Kasteelpark ARENBERG nr. 44 } B-3001 Heverlee-Leuven, Belgium } **************************************************************** } Phone: +32-16-32.1241 - office } +32-16-32.1264 - secretary of department } Fax: +32-16-32.1992 or +32-16-32.1270 } e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be } **************************************************************** } } At 14:13 12/07/02 -0500, you wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hi, } } } } I have a question: With TEM how can I be sure that the feature I am } } looking at } } is/are dislocation(s)? Can you suggest any reading on dislocation } } imaging..Thanks } } } } Aman Haque } } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } Aman Haque } } Dept of Mechanical & Industrial Engg } } University of Illinois at Urbana Champaign } } } } Tel: 217-244-2760, Fax: 217-244-6534
-- Dr. Steven Celotto Department of Engineering Materials Science & Engineering University of Liverpool Brownlow Hill Liverpool L69 3BX UNITED KINGDOM
One of our investigators wants to do TEM on a very minute quantity of sperm, 40 of them to be exact. Does anyone have any suggestions for preparing such a minute sample with the goal of actually being able to find them upon ultrathin sectioning? So far we have tried suspending them in a tiny drop of BSA, crosslinking the BSA with glutaraldehyde, and treating it like a piece of tissue from that point on. Unfortunately, looking for the sperm in the resulting tissue block is like trying to find a pollywog in an ocean. Any helpful hints out there?
Thanks for your help.
Dotty
Dotty Sorenson Microscopy and Image Analysis Laboratory Department of Cell and Developmental Biology University of Michigan Medical School Ann Arbor, Michigan (734)763-1170 FAX (734)763-1166 dsoren-at-umich.edu c
Princeton University's Department of Molecular Biology is seeking a Transmission Electron Microscopist to run a new LEO912AB. Duties will include: (1) teaching and training undergraduate and graduate students, post-docs and faculty on instrument operation, (2) preparation and sectioning of diverse biological specimens including yeast, virally infected mammalian cells, brain slices and Drosophila embryos, using a variety of methods including high-pressure freezing and freeze substitution techniques, (3) daily microscope system checks and scheduling of users, (4) general lab maintenance and supply. The candidate should have a BS/MS in a biological field and several years of TEM experience. Knowledge of ultra-structural and immuno-labeling protocols, the operation and care of microtomes and other equipment needed for specimen preparation, a basic understanding of digital imaging and the ability to manage and administer digital workstations and image archives is essential. The candidate must be highly interactive, willing to collaborate on diverse projects and able to identify and research the best methods of specimen preparation and examination. Rank and salary are dependent upon qualifications and experience. Please send curriculum vitae, a list of references and representative samples of your work to Professor Mark Rose, Chair, Search Committee, Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014. Princeton University is an Equal Opportunity/Affirmative Action Employer
Joe Goodhouse Confocal / EM Core Lab Manager Department of Molecular Biology Princeton University
Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
It is with sadness that I learned today of the death on July 10th of Walter McCrone. Many of you will know of his work in the area of applied light microscopy, particuliarly in the area of materials analysis and problem solving as well as the number of books/short course etc.. which bear his name .
Additional details about his life and work at the following WWW Site
http://www.mccrone.org/WalterMcCrone.html
Walter McCrone is survived by his wife, Lucy, who is also an accomplished microscopist.
Contributions can be made in his name to the Walter C. McCrone Scholarship Fund for Advanced Microscopy Studies, c/o McCrone Research Institute, 2820 S. Michigan Avenue, Chicago, IL 60616.
Sounds like you either have a dead detector or the front end processing electronics have failed. Dead time of 99% indicates something is radically wrong. We had a similar situation recently with a EDX detector. The cause was found to be a cracked detector window.
Peter
-----Original Message----- } From: Yuquan Ding [mailto:yding-at-uwaterloo.ca] Sent: Tuesday, July 16, 2002 3:51 PM To: microscopy-at-sparc5.microscopy.com
Dear Listers:
The last year we installed ENCA 200 system in our JSM 840 SEM. Last week when we repaired our SEM, liquid nitrogen in dewar was emptied to remove water. Then we re-filled new liquid nitrogen through "warm up" and "cool down" procedures. After that, we tried to calibrate this ISIS system. When making "discriminators only", a message showed "ISIS calibration unable to measure strobe peak. Abandoning calibration". At somebody's suggestion, several times of conditioner were conducted, but the situation is the same. We couldn't do anything now. The system seems to be locked. Also when running EDS, no any peaks could be found, showing very high deadtime (99%). Even without beam, the deadtime is also very high. It was suspected that the detector system was damaged. I suspect that the strobed zero peak is generated in the XP2 pulse processor, not in the detector. Why "discriminators only" didn't work?
If anybody of you has experience with this type of problems, I would like to have your opinions and thoughts. Thanks!
Our system No. 05426-2390-414-51, Model 6506, att 1: 32804c802, att2: 0120024, window: ATW2 det. area 10 mmxmm.
Yuquan Ding Materials Labs Dept. of Mechanical Engineering University of Waterloo Waterloo, ON N2L 3G1 519-888-4567 x3766 Fax: 519-888-6197 Email: yding-at-uwaterloo.ca
Just a short comment: the Fortran (i.e. the punch card) format is not awkward at all. Besides, as long as the graphics is available in Fortran too, I don't see why we should abandon the good old language which is still very appropriate for scientific "FORmula TRANslation". There are a huge amount of very good programs written in that language, still in use in the scientific world. As an evidence I can mention a version of the codes given in the book of Head et al., I have written myself, in Fortran, with screen display of the graphica result included (written in Fortran too). Its advantage is that it can be run even on old computers, under DOS. And they are still a lot of "old" computers all over the world.
Corneliu Sarbu, PhD Department of Metallurgy and Applied Materials Science (MTM Dept.) Catholic University of Leuven (KULeuven) Kasteelpark ARENBERG nr. 44 B-3001 Heverlee-Leuven, Belgium **************************************************************** Phone: +32-16-32.1241 - office +32-16-32.1264 - secretary of department Fax: +32-16-32.1992 or +32-16-32.1270 e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be ****************************************************************
At 20:45 16/07/02 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
----- Original Message ----- } From: "Steven Celotto" {s.celotto-at-liverpool.ac.uk} Cc: "Microscopy (E-mail)" {microscopy-at-sparc5.microscopy.com} Sent: Tuesday, July 16, 2002 9:45 PM
Perhaps I was not clear in saying what was awkward. It is their input format being like that of a punch-card, not the Fortran language. When I used their programs I had to make ascii/text files with the program parameters (beam direction, line direction, operating, w, anno etc) in the 82 (?) character line like when they had to input the data via punch cards. It was easy for them because because they were 'brought up' with that format. I was amazed that they could glance through the almost continuous strings of text and find which parameter they wanted (and my mistakes). I had to count characters all the time.
Corneliu Sarbu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Just a short comment: the Fortran (i.e. the punch card) format is not } awkward at all. Besides, as long as the graphics is available in Fortran } too, I don't see why we should abandon the good old language which is still } very appropriate for scientific "FORmula TRANslation". There are a huge } amount of very good programs written in that language, still in use in the } scientific world. As an evidence I can mention a version of the codes given } in the book of Head et al., I have written myself, in Fortran, with screen } display of the graphica result included (written in Fortran too). Its } advantage is that it can be run even on old computers, under DOS. And they } are still a lot of "old" computers all over the world. } } Corneliu Sarbu, PhD } Department of Metallurgy and Applied Materials Science (MTM Dept.) } Catholic University of Leuven (KULeuven) } Kasteelpark ARENBERG nr. 44 } B-3001 Heverlee-Leuven, Belgium } **************************************************************** } Phone: +32-16-32.1241 - office } +32-16-32.1264 - secretary of department } Fax: +32-16-32.1992 or +32-16-32.1270 } e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be } **************************************************************** } } At 20:45 16/07/02 +0100, you wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } OneDis is the program developed by Head et al. It may be still in Fortran } } and it may still have its awkward input format (emulating the old punch } } card format). Prof. Veli-Tapani Kuokkala has repackaged the code into a } } user-friendly Windows format for PC. The program can still be download } } from his webpage. } } } } http://www.tut.fi/units/ms/elm/enindex.htm } } } } "Walck, Scott D." wrote: } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } Your posting reminded me of the software available at the MSA/MAS } } library. There is a public domain software program that is called } } "oneDis" (I think) that will simulate the image of a dislocation after } } providing the program with the imaging parameters. I would recommend } } that you donwload the software and "play" with it to learn what } } dislocations look like at different imaging conditions. It is quite } } useful and it will help with the original question that was posted. } } } } } } You can get to the EMMPDL library from the MSA website. Here are } } instructions that I copied } } } } } } EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National } } Laboratory. The following FTP Site give you access to these Software } } Libraries. Access is by anonymous Login: } } } } } } Host : WWW.AMC.ANL.GOV or } } } the mirror site WWW.MSA.Microscopy.Com } } } } } } Login: } } } Username = Anonymous } } } Password = Your Email Address } } } } } } -Scott } } } } } } Scott D. Walck, Ph.D. } } } PPG Industries, Inc. } } } Glass Technology Center } } } P. O. Box 11472 (letters) } } } Guys Run Rd. (packages) } } } Pittsburgh, PA 15238-0472 } } } } } } Walck-at-PPG.com } } } } } } (412) 820-8651 (office) } } } (412) 820-8515 (fax) } } } } } } -----Original Message----- } } } } From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be] } } } Sent: Sunday, July 14, 2002 1:31 PM } } } To: Microscopy-at-sparc5.microscopy.com } } } Subject: Re: TEM Dislocations } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } The exhaustive treatment of the "classical", two beam diffraction contrast } } } of dislocations in TEM is contained in the book of A.K.Head, P.Humble, } } } L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron } } } micrographs and defect identification". On the ground of the minimum } } } theoretical knowledge that any electron microscopist has to hold, it is } } } presented in all details, computer codes in Fortran included, the method } } } that can generate by computation the "classical" contrast of a rectilinear } } } dislocation and a complex of two dislocations and three stacking faults. } } } There are in use such computer programs, commercial and free ones, that } } } either are applying the codes given in that book or are providing more } } } advanced treatment dealing with the same problem. } } } It is surprising to see that the old "classical" knowledge of the } } } diffraction contrast interpretation has faded away during the HRTEM } } } offensive and that the necessity of looking back to the "simple" two beam } } } diffraction contrast is more and more a request. } } } } } } Corneliu Sarbu, PhD } } } Department of Metallurgy and Applied Materials Science (MTM Dept.) } } } Catholic University of Leuven (KULeuven) } } } Kasteelpark ARENBERG nr. 44 } } } B-3001 Heverlee-Leuven, Belgium } } } **************************************************************** } } } Phone: +32-16-32.1241 - office } } } +32-16-32.1264 - secretary of department } } } Fax: +32-16-32.1992 or +32-16-32.1270 } } } e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be } } } **************************************************************** } } } } } } At 14:13 12/07/02 -0500, you wrote: } } } } ------------------------------------------------------------------------ } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } -----------------------------------------------------------------------. } } } } } } } } } } } } Hi, } } } } } } } } I have a question: With TEM how can I be sure that the feature I am } } } } looking at } } } } is/are dislocation(s)? Can you suggest any reading on dislocation } } } } imaging..Thanks } } } } } } } } Aman Haque } } } } } } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } } } Aman Haque } } } } Dept of Mechanical & Industrial Engg } } } } University of Illinois at Urbana Champaign } } } } } } } } Tel: 217-244-2760, Fax: 217-244-6534 } } } } -- } } Dr. Steven Celotto } } Department of Engineering } } Materials Science & Engineering } } University of Liverpool } } Brownlow Hill } } Liverpool L69 3BX } } UNITED KINGDOM } } } } Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692 } } Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675 } } email: s.celotto-at-liv.ac.uk
-- Dr. Steven Celotto Department of Engineering Materials Science & Engineering University of Liverpool Brownlow Hill Liverpool L69 3BX UNITED KINGDOM
No, as far as I know. I have the Fortran/DOS versions for the cubic, tetragonal and hexagonal versions
in my draw. That is as low as they ever needed. However, they show in their book how to modify the programs for tetragonal and hexagonal crystals. In section 10.6 they explain how they designed the program code so that modify it for other crystals can be done without massive amounts of
rewriting. And they explain how to do it giving the tetragonal and hexagonal versions as examples. On the elastic constants in the ANCALC subroutine and the 'crystallography package' of the code needed to be modified. If someone has the time and energy they could do it.
Ian MacLaren wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear all } Did anyone ever rework these programs to handle dislocations in low symmetry } structures, e.g. monoclinic or triclinic? } } Thanks } } Ian MacLaren } N.B. New address } TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung } Petersenstr. 23, 64287 Darmstadt, Germany } Tel: +49 6151 162894 } ian.maclaren-at-physics.org / http://members.lycos.co.uk/IanMacLaren/ } } ----- Original Message ----- } } From: "Steven Celotto" {s.celotto-at-liverpool.ac.uk} } Cc: "Microscopy (E-mail)" {microscopy-at-sparc5.microscopy.com} } Sent: Tuesday, July 16, 2002 9:45 PM } Subject: Re: TEM Dislocations } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } OneDis is the program developed by Head et al. It may be still in Fortran } and it may still have its awkward input format (emulating the old punch card } format). Prof. Veli-Tapani Kuokkala has repackaged the code into a } user-friendly Windows format for PC. The program can still be download from } his webpage. } } } } http://www.tut.fi/units/ms/elm/enindex.htm } } } } "Walck, Scott D." wrote: } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } Your posting reminded me of the software available at the MSA/MAS } library. There is a public domain software program that is called "oneDis" } (I think) that will simulate the image of a dislocation after providing the } program with the imaging parameters. I would recommend that you donwload } the software and "play" with it to learn what dislocations look like at } different imaging conditions. It is quite useful and it will help with the } original question that was posted. } } } } } } You can get to the EMMPDL library from the MSA website. Here are } instructions that I copied } } } } } } EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National } Laboratory. The following FTP Site give you access to these Software } Libraries. Access is by anonymous Login: } } } } } } Host : WWW.AMC.ANL.GOV or } } } the mirror site WWW.MSA.Microscopy.Com } } } } } } Login: } } } Username = Anonymous } } } Password = Your Email Address } } } } } } -Scott } } } } } } Scott D. Walck, Ph.D. } } } PPG Industries, Inc. } } } Glass Technology Center } } } P. O. Box 11472 (letters) } } } Guys Run Rd. (packages) } } } Pittsburgh, PA 15238-0472 } } } } } } Walck-at-PPG.com } } } } } } (412) 820-8651 (office) } } } (412) 820-8515 (fax) } } } } } } -----Original Message----- } } } } From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be] } } } Sent: Sunday, July 14, 2002 1:31 PM } } } To: Microscopy-at-sparc5.microscopy.com } } } Subject: Re: TEM Dislocations } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } The exhaustive treatment of the "classical", two beam diffraction } contrast } } } of dislocations in TEM is contained in the book of A.K.Head, P.Humble, } } } L.M.Clarebrough, A.J.Morton and C.T.Forwood on "Computed electron } } } micrographs and defect identification". On the ground of the minimum } } } theoretical knowledge that any electron microscopist has to hold, it is } } } presented in all details, computer codes in Fortran included, the method } } } that can generate by computation the "classical" contrast of a } rectilinear } } } dislocation and a complex of two dislocations and three stacking faults. } } } There are in use such computer programs, commercial and free ones, that } } } either are applying the codes given in that book or are providing more } } } advanced treatment dealing with the same problem. } } } It is surprising to see that the old "classical" knowledge of the } } } diffraction contrast interpretation has faded away during the HRTEM } } } offensive and that the necessity of looking back to the "simple" two } beam } } } diffraction contrast is more and more a request. } } } } } } Corneliu Sarbu, PhD } } } Department of Metallurgy and Applied Materials Science (MTM Dept.) } } } Catholic University of Leuven (KULeuven) } } } Kasteelpark ARENBERG nr. 44 } } } B-3001 Heverlee-Leuven, Belgium } } } **************************************************************** } } } Phone: +32-16-32.1241 - office } } } +32-16-32.1264 - secretary of department } } } Fax: +32-16-32.1992 or +32-16-32.1270 } } } e-mail: Corneliu.Sarbu-at-mtm.kuleuven.ac.be } } } **************************************************************** } } } } } } At 14:13 12/07/02 -0500, you wrote: } } } } } ------------------------------------------------------------------------ } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } -----------------------------------------------------------------------. } } } } } } } } } } } } Hi, } } } } } } } } I have a question: With TEM how can I be sure that the feature I am } } } } looking at } } } } is/are dislocation(s)? Can you suggest any reading on dislocation } } } } imaging..Thanks } } } } } } } } Aman Haque } } } } } } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } } } Aman Haque } } } } Dept of Mechanical & Industrial Engg } } } } University of Illinois at Urbana Champaign } } } } } } } } Tel: 217-244-2760, Fax: 217-244-6534 } } } } -- } } Dr. Steven Celotto } } Department of Engineering } } Materials Science & Engineering } } University of Liverpool } } Brownlow Hill } } Liverpool L69 3BX } } UNITED KINGDOM } } } } Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692 } } Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675 } } email: s.celotto-at-liv.ac.uk } } } } } }
-- Dr. Steven Celotto Department of Engineering Materials Science & Engineering University of Liverpool Brownlow Hill Liverpool L69 3BX UNITED KINGDOM
Hmm, must be a Canadian problem, because I have a somewhat similar situation. We also have an Inca system with a SATW window. The JEOL serviceman was just here for the routine on our 5600, and when he vented the column and started taking it apart, we noticed a thin film of condensation on the outside portion of the detector finger. The metal here is just cool to the touch - certainly not cold enough to freeze. We didn't have any significant increase in boiling of the LN2, and consumption is pretty much the rate it has always been. At first, I wasn't too alarmed (humidity is nearly 100% in the Maritime summer), but then I got to thinking (dangerous ground, I know) - why does this only occur when the scope is vented? I've never noticed this condensation before, but normally the detector is inserted fully into the column, and I don't see much of the finger on the outside of the column in that instance.
I'm thinking that we have a pinhole leak or entirely broken window, and I hadn't noticed it before because I normally do specimen exchanges very quickly, and use the EDS relatively infrequently.
With the scope pumped back down, we got the same high deadtime, no peaks in the spectra behavior described below, but this subsided after a few minutes. Low energy peaks were somewhat depressed, but were restored after running the conditioning routine. Strangely, I've had this high deadtime, no peak behavior before, even a month or so after installation, usually shortly after specimen exchange. My questions to this oh so experienced and wise group are:
1. Do I have a leak in the window? I probably know the answer to this one.
2. What are the long term effects on the detector of operating in this manner? With normal use, I'm not so far appreciably affecting pumpdown time of the scope, so I think with brief exchanges I'm not condensing that much inside the scope.
3. We use the EDS system fairly infrequently. Would it be better to warm the system up and only cool it down when we need it? Or would it be better to keep it cold and run the conditioning circuit before use?
Any help would be greatly appreciated. I'd like to dash right out and fix the thing - but there is absolutely no money in the budget in the forseeable future for such a repair. Any brave souls out there who have attempted repairing broken windows themselves?
Thanks in advance,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
We have had similar problems with an 18month old Inca system this year. In our case it was a cracked window and dead FET. The detector had to go back to Oxford for repair.
Alan
At 03:50 PM 7/16/2002 -0400, Yuquan Ding wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Alan W Nicholls, PhD Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 100 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
} } } Hello, } } One of our investigators wants to do TEM on a very minute quantity of } sperm, 40 of them to be exact. Does anyone have any suggestions for } preparing such a minute sample with the goal of actually being able to } find them upon ultrathin sectioning? So far we have tried suspending them } in a tiny drop of BSA, crosslinking the BSA with glutaraldehyde, and } treating it like a piece of tissue from that point on. Unfortunately, } looking for the sperm in the resulting tissue block is like trying to find } a pollywog in an ocean. Any helpful hints out there? } } Thanks for your help. } } Dotty ************************ Hi Dotty, Your question is most timely from my perspective. I just ran up two samples of sperm for a colleague. Initially, we tried agar, with the same results you had with the BSA. My second, successful try consisted simply of spinning the little guys down in an eppendorf tube. Granted, I was using a 200 microliter suspension of sperm in fix, and had many more than 40, but this worked well. I have a small, table top centrifuge that I used at around 900g to bring them to a pellet each time I changed solutions, and then an old Beckman ultrafuge that holds the tubes horizontally to bring the pellet to the very bottom when I put them into the resin. After osmium, I could actually see the sample. With so few, you might want to pellet them first, and then overlay them with a very small volume of the BSA, just to hold them in place.
Good luck! Lee
-- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
I've never heard about an adaptation of this program for a lower symmetry than the tetragonal one (which can be found in the original book by Head et al.). As one who has implemented the Onedis code I have the feeling that it is not very difficult to adapt it for such a lower symmetry. You have just to look at the subroutines that are involved in the calculation of the mechanical strain field and do the necessary modifications. The components of that strain field are much more numerous as the symmetry gets lower, but it can be done. The code given in the book of Head et al. is a good starting point, in as much as the basic principles of the calculations of each of the subroutines are thoroughly presented.
Corneliu Sarbu!
At 11:27 17/07/02 +0200, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
James, It sure sounds like you have a pinhole leak in your window. We developed one in our Kevex Quantum detector on a JEOL 840A years ago. We only noticed it when we had to vent the whole chamber for large samples. We normally used the airlock and so the specimen chamber and detector were kept under vacuum.
However, once we vented the column, we also vented the detector, at least partially. That led to 99% deadtime until the air molecules could be pumped back out the pinhole. Then things seemed to operate fine. I suppose a person could keep on operating like that as routine, but contamination might eventually build up on the crystal. We bit the bullet and shipped the detector back for a window replacement that cost us somewhere between $2000 and 3000. It has been fine these many years since.
We normally see droplets of oil build up on our detector snout over time. (That has been discussed here before.) After several months we take the detector off the scope and clean it. We drizzle freon down the snout and over the window. But you should check with your manufacturer to make sure you don't do anything to damage your window (more) or dissolve the cement holding the window in place.
I suppose water droplets could just as well build up. Iowa is humid, too, but we vent with dry nitrogen and normally don't leave the chamber open all that long, and the snout is not all that cool to the touch.
Warren
At 08:51 AM 7/17/02 -0300, you wrote: } ------------------------------------------------------------------------ } } Hmm, must be a Canadian problem, because I have a somewhat similar situation. } We also have an Inca system with a SATW window. The JEOL serviceman } was just here for the routine on our 5600, and when he vented the column and } started taking it apart, we noticed a thin film of condensation on the outside } portion of the detector finger. The metal here is just cool to the touch - } certainly } not cold enough to freeze. We didn't have any significant increase in } boiling of the } LN2, and consumption is pretty much the rate it has always been. At first, } I wasn't } too alarmed (humidity is nearly 100% in the Maritime summer), but then I } got to } thinking (dangerous ground, I know) - why does this only occur when the } scope is } vented? I've never noticed this condensation before, but normally the } detector is } inserted fully into the column, and I don't see much of the finger on the } outside of } the column in that instance. } } I'm thinking that we have a pinhole leak or entirely broken window, and I } hadn't } noticed it before because I normally do specimen exchanges very quickly, } and use } the EDS relatively infrequently. } } With the scope pumped back down, we got the same high deadtime, no peaks } in the spectra behavior described below, but this subsided after a few } minutes. Low } energy peaks were somewhat depressed, but were restored after running the } conditioning } routine. Strangely, I've had this high deadtime, no peak behavior before, } even a month } or so after installation, usually shortly after specimen exchange. My } questions to this oh so } experienced and wise group are: } } 1. Do I have a leak in the window? I probably know the answer to this one. } } 2. What are the long term effects on the detector of operating in this manner? } With normal use, I'm not so far appreciably affecting pumpdown time of the } scope, } so I think with brief exchanges I'm not condensing that much inside the scope. } } 3. We use the EDS system fairly infrequently. Would it be better to warm } the system } up and only cool it down when we need it? Or would it be better to keep } it cold and run the conditioning circuit before use? } } Any help would be greatly appreciated. I'd like to dash right out and fix } the thing - but } there is absolutely no money in the budget in the forseeable future for } such a repair. } Any brave souls out there who have attempted repairing broken windows } themselves? } } Thanks in advance, } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/~jehrman } } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Dear Listers: } } } } The last year we installed ENCA 200 system in our JSM 840 SEM. Last week } } when we repaired our SEM, liquid nitrogen in dewar was emptied to remove } } water. Then we re-filled new liquid nitrogen through "warm up" and "cool } } down" procedures. After that, we tried to calibrate this ISIS system. When } } making "discriminators only", a message showed "ISIS calibration unable to } } measure strobe peak. Abandoning calibration". At somebody's suggestion, } } several times of conditioner were conducted, but the situation is the same. } } We couldn't do anything now. The system seems to be locked. Also when } } running EDS, no any peaks could be found, showing very high deadtime (99%). } } Even without beam, the deadtime is also very high. It was suspected that the } } detector system was damaged. I suspect that the strobed zero peak is } } generated in the XP2 pulse processor, not in the detector. Why } } "discriminators only" didn't work? } } } } If anybody of you has experience with this type of problems, I would like to } } have your opinions and thoughts. Thanks! } } } } Our system No. 05426-2390-414-51, Model 6506, att 1: 32804c802, att2: } } 0120024, window: ATW2 det. area 10 mmxmm. } } } } Yuquan Ding } } Materials Labs } } Dept. of Mechanical Engineering } } University of Waterloo } } Waterloo, ON N2L 3G1 } } 519-888-4567 x3766 } } Fax: 519-888-6197 } } Email: yding-at-uwaterloo.ca
------------------------------------------- No files should be attached to this message ------------------------------------------- Warren E. Straszheim, Ph.D. Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
If I may be so bold to stick my nose into what is normally Nestor's business, I offer these sections taken from the list FAQ. The link to the FAQ is given at the top of each posting.
Since Nestor's reminder to unsubscribe from the list rather than to simply return "out of office" messages during absences, there have been a few commands sent to the list to unsubscribe or to switch to digest mode. That isn't the way to do it.
First, the list itself does not handle commands. Some implementations of mailing lists do - many do not. This list simply is the latter form. See note #2 below.
Second, there is no digest mode at this time. There may be later and I am sure Nestor will tell us if digest mode becomes available.
Thanks, Nestor, for all your investment in this list. It is a wonderful aid.
Warren ------------------------------ How do I unsubscribe?
Send an Email Message to "Listserver-at-MSA.Microscopy.Com" in the body of your message include the line Unsubscribe Microscopy "USERID-at-EMailAddress" Replace the "USERID-at-EMailAddress" with your real Email address. Or use our WWW based form at: http://www.msa.microscopy.com/MicroscopyListserver/UnSubscribeMicroscopy.html IMPORTANT NOTE#1. To unsubscribe you must supply the original address which you used when you first submitted your subscription. Over the last few years I have found that many users have their mail forwarded to new addresses, or use an alias. When they then try to unsubscribe, they forget where there mail is being sent to (or forwarded from). This causes me lots of headaches. So just remember in order to unsubscribe you from the list, the listserver must know the your original subscription address to remove it! Please be considerate. I am not a mindreader, and don't really enjoy playing detective over the Internet. I have too many other things to do. IMPORTANT NOTE #2: Do not send subscribe/unsubscribe message to "Microscopy-at-MSA.Microscopy.Com". When you do this ALL members of the listserver receive your message. All administrative issues should be sent to "Listserver-at-MSA.Microscopy.Com" and not to the general mailing list.
Is there a Digest Mode?
Digest Mode is not available on the present server. It is an option which will be added in the future. Digest Mode compresses all message from a single day into one long message which is then sent to all subscribers. It is a nice option, and I intend to get it running eventually.
------------------------------------------- No files should be attached to this message ------------------------------------------- Warren E. Straszheim, Ph.D. Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
Dorothy, Some possibilities a. fix and wick the cells into a cellulose fiber similar to the one produced by Microdyn (microfiltration cartridges)--will send website directly b. fix and drop cells onto a disk of nitrocellulose paper, add second disk of np to sandwich cells and process in wells of a staining dish c. filter using polycarbonate filer, fix, process, flat embedd or use a Chen mold
Rosemary At 04:37 PM 7/16/02 -0400, Dorothy Roak Sorenson wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I'm in a mountain cabin in Utah, looking at my e-mail on a laptop.
That's a tough problem. I could imagine something like the following: Make up plastic embedding monomer and add a little dye to it. Polymerize a block of it and then make tiny shavings or particles of it. Put some sticky substance (polylysine, etc.?) on the surface of the shavings. Put one or more shaving in with the sperm in a way that would cause the sperm to attach to the shaving(s). You would have to decide whether the sperm should be fixed before or after they attached to the shaving. When fixed sperm are on the shaving(s), dehydrate them, and then put a shaving or tight cluster of shavings into embedding medium at one end of a flat embedding block. Polymerize the block. You should be able to see the shaving(s) because of the dye, and can section them and see the sperm that are on theeir surface.
Kent
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A. Kent Christensen, Professor Emeritus Department of Cell and Developmental Biology, Medical Science II Building University of Michigan Medical School, Ann Arbor, MI 48109-0616 Tel (work) (734) 763-1287, Fax (work) (734) 763-1166 akc-at-umich.edu http://www.umich.edu/~akc/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, } } One of our investigators wants to do TEM on a very minute quantity of } sperm, 40 of them to be exact. Does anyone have any suggestions for } preparing such a minute sample with the goal of actually being able to } find them upon ultrathin sectioning? So far we have tried suspending them } in a tiny drop of BSA, crosslinking the BSA with glutaraldehyde, and } treating it like a piece of tissue from that point on. Unfortunately, } looking for the sperm in the resulting tissue block is like trying to find } a pollywog in an ocean. Any helpful hints out there? } } Thanks for your help. } } Dotty } } } Dotty Sorenson } Microscopy and Image Analysis Laboratory } Department of Cell and Developmental Biology } University of Michigan Medical School } Ann Arbor, Michigan } (734)763-1170 } FAX (734)763-1166 } dsoren-at-umich.edu } c }
You might try mixing a small drop of stain such as Toluidine Blue in the agar for easier visualization of your sample.
At 04:37 PM 7/16/02 -0400, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Larry Ackerman Howard Hughes Medical Institute UCSF, Box 0725, Rm U226 533 Parnassus Avenue San Francisco, CA 94143 USA
-----Original Message----- } From: Dorothy Roak Sorenson [mailto:dsoren-at-umich.edu] Sent: Tuesday, July 16, 2002 1:38 PM To: microscopy-at-sparc5.microscopy.com
Hello,
One of our investigators wants to do TEM on a very minute quantity of sperm, 40 of them to be exact. Does anyone have any suggestions for preparing such a minute sample with the goal of actually being able to find them upon ultrathin sectioning? So far we have tried suspending them in a tiny drop of BSA, crosslinking the BSA with glutaraldehyde, and treating it like a piece of tissue from that point on. Unfortunately, looking for the sperm in the resulting tissue block is like trying to find a pollywog in an ocean. Any helpful hints out there?
Thanks for your help.
Dotty
Dotty Sorenson Microscopy and Image Analysis Laboratory Department of Cell and Developmental Biology University of Michigan Medical School Ann Arbor, Michigan (734)763-1170 FAX (734)763-1166 dsoren-at-umich.edu c
Hi Everybody! I was wondering if any of you have used Hepes buffer for electron microscopy fixation. We used 2% glut in Hepes and we found fixation unsatisfactory. Did any of you experienced any problems using this system? Thanks Dorota
A flat embedding problem. We have cryofixed and cryosubstituted some Pisolithus tinctorius (a fungi) for one of our users and have run into a a bit of a problem.
We cryosubsituted in 2% Osmium Tetroxide in Acetone for 72 hours in a Leica AFS. After gradually bringing the samples to room temperature and rinsing in absolute acetone we very slowly infiltrated into Quetol 651 resin (formulation used; Quetol 651 10 g, NSA 10g, MNA 10g DMP-30 0.45g). We then flat embedded the fungi between two sheets of Melinex film (Agar Scientific catalogue number L4103) and polymerised overnight at 60oC.
We have used Melinex film on numerous occasions in the past to flat embed vibratomed sectioned brain slices, monolayers of culture cells etc. We have used Melinex film with 'conventional' epoxy resins (ie TAAB TK3, Agar 100 resin) and with LR White. We have not in the past used Melinex with Quetol 651.
When we came to seperate the Melinex to remove the flat embedded fungi the Melinex crazed and fractured , it wouldn't peel apart.
We ran a trial with old stock Melinex and some fairly new stuff. Not wanting to get caught again we also trialed with some Alcar, Thermonx and Mylar film. We tried the Quetol 651 again and some Agar 100.
Interestingly the Meinex (both the old stock and new stuff) reacted in the same way both with the Quetol and the Agar 100 (which has nevcer happened to us before). The Alcar, Thermonx and Mylar film were all fine with the two resins.
Is, or has, anyone else out experienced the same problem and come up with an answer ?
While on the subject, does anybody know at what temperature the osmium in the acetone based cryosubstitution medium actual fixes at ?
It is my understanding that the idea is that the acetone/ osmium fully infiltrates the tissue at the lower temperature, replacing the cell water, but the osmium doesn't actually fix until the sample is warmed up to a particular temperature (warmed up still being somewhat lower than zero degress C).
Thanks in advance
Allan
-- ------------------------------------------------- Allan Mitchell Technical Manager Otago Centre for Electron Microscopy C/-Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
We have a student interested in the ultrastructure of boab nuts....can anyone help with the interpretation of micrographs?
cheers Sally
Dr Sally Stowe Facility Coordinator, ANU Electron Microscopy Unit Research School of Biological Sciences Australian National University Canberra ACT0200 AUSTRALIA stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218 ph 02 6125 2743 http://www.anu.edu.au/EMU
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id IAA26258 for dist-Microscopy; Thu, 18 Jul 2002 08:50:26 -0500 (CDT) Received: from njz_spm_filter (sparc5 [206.69.208.10]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id IAA26250 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Thu, 18 Jul 2002 08:49:56 -0500 (CDT) Received: from magic.wcupa.edu (magic.wcupa.edu [144.26.94.241]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with ESMTP id IAA26243 for {Microscopy-at-sparc5.microscopy.com} ; Thu, 18 Jul 2002 08:49:44 -0500 (CDT) Received: from mail.WCUPA.EDU (mail.wcupa.edu [144.26.92.199]) by magic.wcupa.edu (8.11.2/8.11.2) with ESMTP id g6IDj4W09403; Thu, 18 Jul 2002 09:45:05 -0400 (EDT) Received: by mail.wcupa.edu with Internet Mail Service (5.5.2650.21) id {3N5PX6XC} ; Thu, 18 Jul 2002 09:44:13 -0400 Message-ID: {B47C27775351D41197DC00805FEA78A70602DD41-at-mail.wcupa.edu}
Morning,
When you changed your buffer from the old(?) to HEPES, what change, if any, did you record in its osmolarity? Also, there ARE non-trivial effects recorded for different buffering systems on various tissues. Every change should be followed by an experiment to define new optima for the tissues under consideration, and the best way to determine new optima is to start with semi-thin section comparisons followed by ultrastructural analysis.
If it was easy, we'd all be paid much more for our work.
Regards,
Fred Monson
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center West Chester University South Church Street and Rosedale West Chester, Pennsylvania, USA, 19383 Phone: 610-738-0437 FAX: 610-738-0437 fmonson-at-wcupa.edu CASI URL: http://darwin.wcupa.edu/casi/ WCUPA URL: http://www.wcupa.edu/ Visitors URL: http://www.wcupa.edu/_visitors/
} ---------- } From: Dorota Wadowska } Sent: Wednesday, July 17, 2002 2:54 PM } To: microscopy-at-sparc5.microscopy.com } Subject: TEM-hepes buffer } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi Everybody! } I was wondering if any of you have used Hepes buffer for electron } microscopy fixation. We used 2% glut in Hepes and we found } fixation unsatisfactory. Did any of you experienced any problems } using this system? } Thanks } Dorota } }
I've used HEPES buffer with eubacteria, cyanobacteria, several algae (green and golden brown) and land plants with good success. I did a side-by-side comparison of cacodylate, phosphate and PIPES (a different organic buffer) early in my career - an increasingly more scary number of years ago - and PIPES was by far the best. I tried HEPES on the advice of a collegue that worked on dinoflagellates, and have been using it ever since. I have not had much experience with animal tissue, with the exception of some tissue cultured cells. We have used HEPES in these situations when it was the buffer system the cells were grown in, and have had no trouble with it.
Heather Owen
Dr. Heather A. Owen, Director Electron Microscope Laboratory Department of Biological Sciences University of Wisconsin - Milwaukee Lapham Hall, P.O. Box 413 Milwaukee, WI 53210 USA
Electron Microscopy Core Facility at the Yale University School of Medicine Seeks experienced electron microscopist to assist Principal Investigator and other research scientists as consultant for independent experiments; overseeing the operation of the facility, preparation of specimens, conducting immunocytochemical labeling, sample analysis and statistical analysis. Candidate will be expected to conduct independent research projects in consultation of PI; design experiments, interpret results and present proposals for future experiments. Will also supervise lab personnel and other users of the facility as well as teach EM techniques to users on a 1:1 basis or formal courses organized biannually.
Position requires a Master’s degree in the biological sciences and one year prior experience in related position; or the equivalent combination of education and experience. Previous experience in microscopy and in the conduct of independent research is required; also strong computer literacy is required.
Qualified candidates send resume and cover letter referencing source code EASEM10769 to Ms. S. Greer at jobs-at-yale.edu. Fax: 203-432-9817. Mail: PO Box 208344, New Haven, CT 06520-8344. For more information about this position and Yale’s outstanding benefits package visit www.yale.edu.
Yale University is an Affirmative Action, Equal Opportunity Employer.
-- Marc Pypaert Department of Cell Biology, Center for Cell and Molecular Imaging and Ludwig Institute for Cancer Research, Yale University School of Medicine 333 Cedar Street New Haven CT 06520 Tel : (203) 785 3681 Fax : (203) 785 7446
Dorota, We obtained good fixation of Arabidopsis thaliana buds using the protocol from the following publication: Owen HA and Makaroff CA. Ultrastructure of microsporogenesis and microgametogenesis in Arabidopsis thaliana (L.) Heynh. ecotype Wassilewskija (Brassicaceae). Protoplasma 185:7-21, 1995. I don't have concentrations available to me at the moment but the fixatives are fully described in this article. It was a great find for us and we have used it on other plants as well. Rosemary
I am looking for a broken TEM sample holder that you may no longer need. Please contact me if you have one - thanks in advance.
With best regards. ----------------------------------------------------------------- Lu-Chang Qin, ScD Department of Physics and Astronomy & Curriculum in Applied and Materials Sciences University of North Carolina at Chapel Hill Room 178 Phillips Hall, CB#3255 Chapel Hill, NC 27599-3255 Phone: (919) 843-3575
I was surprised to find that some people in EM Labs used 0.1 M phosphate buffer as a "PBS". From my course of biochemistry/cell biology PBS stands for Phosphate Buffered Saline and is 20 mM phosphate buffer, pH 7.4 and 150 mM NaCl. If you replace 20 mM phosphate on 20 mM of any other buffer, HEPES for instance, it's very unlikely the total osmomolarity will changed... BUT, if you are using 0.1 (?) M HEPES it may be a HUGE DIFFERENCE... You have to understand clearly, which buffer system you are using. In biochemistry, we never ever use 100 mM range buffers itself (like 0.1 M phosphate/HEPES/MOPS etc). The purpose of the 'buffer' is to provide necessary pH and sodium or potassium salts were used to provide necessary osmosity... GA itself affect osmosity seriously and formally speaking, osmosity should be corrected. From the 'chemical' point of view, HEPES should not affect GA efficiency. Moreover, HEPES is the best buffer for GA crosslinking because of it's huge buffer capacity in the neutral pH range. As I understand, some 'buffer systems' like cacodylate helps to wash out some content of the cell matrix and therefore makes some structural details more pronounced. Mistakenly, some people called it 'good fixation'. If you are using 100 mM range buffers, such 'washing effect' supposed to be very pronounced. HEPES is good to preserve the matrix integrity, not for washing out it's components. I am sorry for such obvious comment. Best wishes, Sergey.
At 09:44 AM 7/18/02 -0400, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Rather than putting animals (such as mouse pups) under a stereoscope that can detect GFP, has anyone ever heard of a hand-held device that can be used to see if the pups are green?
Thank you in adance...
Lesley
Lesley S. Bechtold Supervisor, Biological Imaging The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6191
I have a project requiring that I identify cells grown in culture that were originally taken from porcine tissue. The cells are suspected to be either smooth muscle cells, endothelium, or fibroblasts. I am unfamiliar with which antibodies would work for these cells. There are many antibodies for these types of cells, but will they work in porcine tissue? Help.....
Not at all Fred. I was commenting the original message, not yours. No worry. I think, we both agree that in order to get good answer, we have to ask good questions. For instance the definition of the 'HEPES buffer' to me mean that people talking about 10-20 mM concentration of HEPES. 0.1 M HEPES is a stock solution to me. Others may think differently. As a result there is misunderstanding may occur (exactly what you mean). Sergey
At 01:29 PM 7/18/02, you wrote: } Did I say something wrong? I tried not to. But I can tell from what you } wrote that we agree. My response was my nasty way to say that there was } insufficient information to draw any conclusion and frame any answer. } } Hope you agree. } } Fred } } } } ---------- } } From: Sergey Ryazantsev } } Sent: Thursday, July 18, 2002 3:02 PM } } To: Monson, Frederick C.; microscopy-at-sparc5.microscopy.com } } Subject: RE: TEM-HEPES buffer } } } } I was surprised to find that some people in EM Labs used 0.1 M phosphate } } buffer as a "PBS". From my course of biochemistry/cell biology PBS } } stands } } for Phosphate Buffered Saline and is 20 mM phosphate buffer, pH 7.4 and } } 150 } } mM NaCl. If you replace 20 mM phosphate on 20 mM of any other buffer, } } HEPES for instance, it's very unlikely the total osmomolarity will } } changed... BUT, if you are using 0.1 (?) M HEPES it may be a HUGE } } DIFFERENCE... You have to understand clearly, which buffer system you are } } } } using. In biochemistry, we never ever use 100 mM range buffers itself } } (like } } 0.1 M phosphate/HEPES/MOPS etc). The purpose of the 'buffer' is to } } provide } } necessary pH and sodium or potassium salts were used to provide necessary } } osmosity... GA itself affect osmosity seriously and formally speaking, } } osmosity should be corrected. From the 'chemical' point of view, HEPES } } should not affect GA efficiency. Moreover, HEPES is the best buffer for } } GA } } crosslinking because of it's huge buffer capacity in the neutral pH range. } } } } As I understand, some 'buffer systems' like cacodylate helps to wash out } } some content of the cell matrix and therefore makes some structural } } details } } more pronounced. Mistakenly, some people called it 'good fixation'. If } } you } } are using 100 mM range buffers, such 'washing effect' supposed to be very } } pronounced. HEPES is good to preserve the matrix integrity, not for } } washing out it's components. I am sorry for such obvious comment. Best } } wishes, Sergey. } } } } At 09:44 AM 7/18/02 -0400, you wrote: } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } Morning, } } } } } } When you changed your buffer from the old(?) to HEPES, what } } change, } } } if any, did you record in its osmolarity? Also, there ARE non-trivial } } } effects recorded for different buffering systems on various tissues. } } Every } } } change should be followed by an experiment to define new optima for the } } } tissues under consideration, and the best way to determine new optima is } } to } } } start with semi-thin section comparisons followed by ultrastructural } } } analysis. } } } } } } If it was easy, we'd all be paid much more for our work. } } } } } } Regards, } } } } } } } } } Fred Monson } } } } } } Frederick C. Monson, PhD } } } Center for Advanced Scientific Imaging } } } Schmucker II Science Center } } } West Chester University } } } South Church Street and Rosedale } } } West Chester, Pennsylvania, USA, 19383 } } } Phone: 610-738-0437 } } } FAX: 610-738-0437 } } } fmonson-at-wcupa.edu } } } CASI URL: http://darwin.wcupa.edu/casi/ } } } WCUPA URL: http://www.wcupa.edu/ } } } Visitors URL: http://www.wcupa.edu/_visitors/ } } } } } } } } } } ---------- } } } } From: Dorota Wadowska } } } } Sent: Wednesday, July 17, 2002 2:54 PM } } } } To: microscopy-at-sparc5.microscopy.com } } } } Subject: TEM-hepes buffer } } } } } } } } } } ------------------------------------------------------------------------ } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } America } } } } To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com } } } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } -----------------------------------------------------------------------. } } } } } } } } } } } } Hi Everybody! } } } } I was wondering if any of you have used Hepes buffer for electron } } } } microscopy fixation. We used 2% glut in Hepes and we found } } } } fixation unsatisfactory. Did any of you experienced any problems } } } } using this system? } } } } Thanks } } } } Dorota } } } } } } } } } } } }
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Does anyone out there have any experience to share about the Philips XL-30 (Sirion or other) with EDAX relative to potential consequences of a diffusion pump versus a turbo? It seems to me that a turbo pump would go a long way to prevent contamination of the detector window. I am not sure about this. The Sirion isolates the chamber from the diffusion pump during specimen exchange. Plus, it has a chilled water trap. So this may not be a problem.
Haven't tried this yet, but a blue fluorescent lamp, maximum emission around 480 or so nm, may excite GFP enough to be visible in a dark room. We're planning to test this to see if we can screen thousands of GFP-expressing seeds. Could observe the fluorescence through a long-pass theatre filter of some kind, like one of the Lee green or yellow filters, which have quite sharp wavelength cutoffs (have used various Lee filters to get different wavelength illumination for prac classes looking at photomorphogenesis in plants). good luck, Rosemary } } } Hi Everyone, } } Rather than putting animals (such as mouse pups) under a } stereoscope that can detect GFP, has anyone ever heard of a hand-held } device that can be used to see if the pups are green? } } Thank you in adance... } } Lesley } } } } Lesley S. Bechtold } Supervisor, Biological Imaging } The Jackson Laboratory } 600 Main St. } Bar Harbor, ME 04609 } 207-288-6191
Rosemary White Microscopy Centre CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia rosemary.white-at-csiro.au fax 61- 2 6246 5000 ph. 61- 2 6246 5475 or mob. 61- 0402 835 973
We have two XL30s. One is 8 years old with a diffusion pump and one is 1 year old with a turbo pump. Both have Edax EDS systems, and we have not seen any evidence of contamination on the x-ray detectors. The vacuum systems on our Philips/FEI microscopes have been very reliable.
Joe Neilly, Research Investigator Abbott Laboratories R45M, AP31 200 Abbott Park Rd. Abbott Park, IL 60064-6202
Voice: 847-938-5024 Fax: 847-938-5027
Gary Gaugler {gary-at-gaugler To: MSA listserver {Microscopy-at-sparc5.microscopy.com} .com} cc: Subject: XL-30 Sirion x-ray & diffusion pump 07/18/02 09:23 PM
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Does anyone out there have any experience to share about the Philips XL-30 (Sirion or other) with EDAX relative to potential consequences of a diffusion pump versus a turbo? It seems to me that a turbo pump would go a long way to prevent contamination of the detector window. I am not sure about this. The Sirion isolates the chamber from the diffusion pump during specimen exchange. Plus, it has a chilled water trap. So this may not be a problem.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dpu-at-spdc.ti.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on Thursday, July 18, 2002 at 13:16:32 ---------------------------------------------------------------------------
Email: dpu-at-spdc.ti.com Name: Dennis Pu
Organization: Texas Instruments
Education: Graduate College
Location: Dallas, Texas 75243
Question: Hello, This is not exactly a question for school, but I hope we're all here to learn and explore. My question deals with using microscopy to view detailed images of the inside of vinyl record grooves. My desire is to research what it might take to build an optical record player. I'm trying to determine whether it is more practical to utilize some kind of optical tracking of the groove for real-time play or if scanning the whole record in at once and using software to recognize the grooves and to produce the sound would be better. My first step is just to determine ways I can use microscopy to even see inside groove. I'd consider one economical option that hobbyists could tinker with at home, and one "best" solution archivists can use for transcribing old media (vinyl, wax cylinders, acetates, etc...) without having to use destructive mechanical means. I'd appreciate any suggestions you have to offer.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cf_reu-at-ccmr.cornell.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday, July 18, 2002 at 10:00:16 ---------------------------------------------------------------------------
Email: cf_reu-at-ccmr.cornell.edu Name: Chris Fanning
Organization: Cornell University
Education: Undergraduate College
Location: Ithaca, Ny, US
Question: I am involved in undergraduate materials research on Nickel-Titanium (50.5% ni). I have prepared an ~1cm cube of nitinol potted in epoxy to be viewed in an SEM. Our goal is to obtain an ODF via an EBSD scan. to accomplish this we need to get well defined patterns in the EBSD. We are getting patterns now but they are not good enough. We are considering etching and need recomendations for etching solutions. Many people suggest an HF solution. Are there alternatives to HF?
Our material prep was, ... -cut with a slow speed (200 rpm) precision saw, diamond blade -rough polish with 240, 320, 480(?), 600, 1200 Si-carbide paper by hand -fine polish with 9u, 3u, 1u on nylon paper - polishing wheel, 200rpm -fine polish with colloidal silica, by hand -4 hrs on vibraory polisher with colloidal silica
any advice would be much appreciated! sorry for the long email,
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (julycola-at-biof.ufrj.br) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday, July 18, 2002 at 09:15:20 ---------------------------------------------------------------------------
Organization: Federal University of Rio de Janeiro
Education: Graduate College
Location: Rio de Janeiro, Brazil
Question: We are not succeeding in preparing 16% formaldehyde from EMS Paraformaldehyde. The final mixture is turbid no matter how much NaOH we add. What could be the cause? We have not had this kind of trouble in many years of preparation. Do you advise pH adjustment of the freshly prepared solution? Thank You for the help.
I disagree with your comment that a buffer has to be used in the 10-20 mM range, and that at 0.1 M it should be considered a stock solution. Clearly the concentration has to be proportional to the buffering capacity required. When using large concentrations of PFA (4-8 %), high concentration of HEPES is required to maintain the pH neutral. We and others use routinely 250 mM Hepes "buffer" with PFA fixation and get great morphology (as defined by an absence of swelling of internal membranes and lack of extraction of cytoplasmic components). Similarly, I know of many EM groups who use routinely 100 or 200 mM phosphate solutions for fixation for both GA and PFA fixation. As Fred said, what matters is to try various techniques and identify the optimal conditions. Of course, what someone calls great fixation can be seen as poor fixation by someone else. Since nobody really knows what a cell is supposed to look like at the microscopical level without having to kill it first, remove all its water content and saturate it with heavy metals, discussing what is a good or poor morphology can be a futile exercise. According to what I consider reasonnable criteria (some of which are listed above), I and others have found that fixation in 200 or 250 mM solutions of HEPES, Pipes or phosphate actually works well. Best regards
Marc
Sergey Ryazantsev wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Not at all Fred. I was commenting the original message, not yours. No } worry. I think, we both agree that in order to get good answer, we have to } ask good questions. For instance the definition of the 'HEPES buffer' to } me mean that people talking about 10-20 mM concentration of HEPES. 0.1 M } HEPES is a stock solution to me. Others may think differently. As a result } there is misunderstanding may occur (exactly what you mean). Sergey } } At 01:29 PM 7/18/02, you wrote: } } Did I say something wrong? I tried not to. But I can tell from what you } } wrote that we agree. My response was my nasty way to say that there was } } insufficient information to draw any conclusion and frame any answer. } } } } Hope you agree. } } } } Fred } } } } } } } ---------- } } } From: Sergey Ryazantsev } } } Sent: Thursday, July 18, 2002 3:02 PM } } } To: Monson, Frederick C.; microscopy-at-sparc5.microscopy.com } } } Subject: RE: TEM-HEPES buffer } } } } } } I was surprised to find that some people in EM Labs used 0.1 M phosphate } } } buffer as a "PBS". From my course of biochemistry/cell biology PBS } } } stands } } } for Phosphate Buffered Saline and is 20 mM phosphate buffer, pH 7.4 and } } } 150 } } } mM NaCl. If you replace 20 mM phosphate on 20 mM of any other buffer, } } } HEPES for instance, it's very unlikely the total osmomolarity will } } } changed... BUT, if you are using 0.1 (?) M HEPES it may be a HUGE } } } DIFFERENCE... You have to understand clearly, which buffer system you are } } } } } } using. In biochemistry, we never ever use 100 mM range buffers itself } } } (like } } } 0.1 M phosphate/HEPES/MOPS etc). The purpose of the 'buffer' is to } } } provide } } } necessary pH and sodium or potassium salts were used to provide necessary } } } osmosity... GA itself affect osmosity seriously and formally speaking, } } } osmosity should be corrected. From the 'chemical' point of view, HEPES } } } should not affect GA efficiency. Moreover, HEPES is the best buffer for } } } GA } } } crosslinking because of it's huge buffer capacity in the neutral pH range. } } } } } } As I understand, some 'buffer systems' like cacodylate helps to wash out } } } some content of the cell matrix and therefore makes some structural } } } details } } } more pronounced. Mistakenly, some people called it 'good fixation'. If } } } you } } } are using 100 mM range buffers, such 'washing effect' supposed to be very } } } pronounced. HEPES is good to preserve the matrix integrity, not for } } } washing out it's components. I am sorry for such obvious comment. Best } } } wishes, Sergey. } } } } } } At 09:44 AM 7/18/02 -0400, you wrote: } } } } ------------------------------------------------------------------------ } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } -----------------------------------------------------------------------. } } } } } } } } } } } } Morning, } } } } } } } } When you changed your buffer from the old(?) to HEPES, what } } } change, } } } } if any, did you record in its osmolarity? Also, there ARE non-trivial } } } } effects recorded for different buffering systems on various tissues. } } } Every } } } } change should be followed by an experiment to define new optima for the } } } } tissues under consideration, and the best way to determine new optima is } } } to } } } } start with semi-thin section comparisons followed by ultrastructural } } } } analysis. } } } } } } } } If it was easy, we'd all be paid much more for our work. } } } } } } } } Regards, } } } } } } } } } } } } Fred Monson } } } } } } } } Frederick C. Monson, PhD } } } } Center for Advanced Scientific Imaging } } } } Schmucker II Science Center } } } } West Chester University } } } } South Church Street and Rosedale } } } } West Chester, Pennsylvania, USA, 19383 } } } } Phone: 610-738-0437 } } } } FAX: 610-738-0437 } } } } fmonson-at-wcupa.edu } } } } CASI URL: http://darwin.wcupa.edu/casi/ } } } } WCUPA URL: http://www.wcupa.edu/ } } } } Visitors URL: http://www.wcupa.edu/_visitors/ } } } } } } } } } } } } } ---------- } } } } } From: Dorota Wadowska } } } } } Sent: Wednesday, July 17, 2002 2:54 PM } } } } } To: microscopy-at-sparc5.microscopy.com } } } } } Subject: TEM-hepes buffer } } } } } } } } } } } } } ------------------------------------------------------------------------ } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } } America } } } } } To Subscribe/Unsubscribe -- Send Email to } } } ListServer-at-MSA.Microscopy.Com } } } } } On-Line Help } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } } -----------------------------------------------------------------------. } } } } } } } } } } } } } } } Hi Everybody! } } } } } I was wondering if any of you have used Hepes buffer for electron } } } } } microscopy fixation. We used 2% glut in Hepes and we found } } } } } fixation unsatisfactory. Did any of you experienced any problems } } } } } using this system? } } } } } Thanks } } } } } Dorota } } } } } } } } } } } } } } } } } } _____________________________________ } } Sergey Ryazantsev Ph. D. } Electron Microscopy } UCLA School of Medicine } Department of Biological Chemistry } Box 951737 } Los Angeles, CA 90095-1737 } } Phone: (310) 825-1144 } FAX (departmental): (310) 206-5272 } mailto:sryazant-at-ucla.edu
-- Marc Pypaert Department of Cell Biology, Center for Cell and Molecular Imaging and Ludwig Institute for Cancer Research, Yale University School of Medicine 333 Cedar Street New Haven CT 06520 Tel : (203) 785 3681 Fax : (203) 785 7446
Renaat The purpose of the peltier cold stage is to cool the specimen, thereby reducing the vapour pressure of any volatile liquids, such as water, oils, that it contains. At the temperatures attainable with a peltier module (perhaps -30oC?) water or ice cannot be completely stabilized, unless you can match the partial pressure of water vapour in the chamber atmosphere to the vapour pressure above ice at the specimen temperature employed. This may require ESEM and may not be possible using LVSEM. However, the rate of outgassing of wet specimens may be greatly reduced. You can also stabilize some low melting point materials, such as waxes, chocolate or butterfat in food samples by reducing their temperature. Chris
} From: "Renaat Dasseville" {renaat.dasseville-at-rug.ac.be} To: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}
Kathy:
Try the SEROTEC company at www.serotec.co.uk or Veterinary Medical Research & Development at www.vmrd.com or Upstate Cell Signaling Solutions at www.upstate.com
Karen L. Bentley, M.S.(previously Jensen) Associate Scientist & Project Manager Electron Microscope Research Core University of Rochester Medical Center Rochester, NY 14642 585-275-1954
-----Original Message----- } From: kwalters [mailto:kwalters-at-blue.weeg.uiowa.edu] Sent: Thursday, July 18, 2002 4:47 PM To: microscopy
Hi All,
I have a project requiring that I identify cells grown in culture that were originally taken from porcine tissue. The cells are suspected to be either smooth muscle cells, endothelium, or fibroblasts. I am unfamiliar with which antibodies would work for these cells. There are many antibodies for these types of cells, but will they work in porcine tissue? Help.....
I don't have experience of the XL30, but so far I know, the XL30 serie uses Edwards Diffstack diff pumps, which are very good pumps, and don't backstream a lot. We use it (with Santovac oil) on UHV surface science instruments without any contamination probleme (and Auger is sensitiv to carbon...). As we know, contamination comes from the rouhing pump, and a turbo pump stops better oil from it than a diff one. The question is : how is the SEM chamber pumped from air pressure down to 1E-2mbar ? Is the diff pump by pased by an oil seeled rouhing pump ? If so, you will have a source of contamination independently from the diff or turbo pump.
To avoid all sources of contamination, you have two designs possible : -a turbo pump backed by a scroll. The two pumps are stopped when the specimen chamber is vented, and are put on together to pumpd down again. I think Leo does it this way. -a fast entry lock, pumped by a scroll pump, and idealy a turbo and a scroll on the chamber, or less expensive, a diff and a rotary vane pump (with alumina foreline trap). I would prefer this design, because I don't thing it's a good idee for a HR-SEM to put the whole chamber to air at each specimen exchange.
What is meaningsless is to put a turbo pump as secondary pump, to avoid contamination and than use a oil sealed pump to pump down the entry lock, or do the primary vaccum with it, by passing the turbo. Of coarse you can put a turbo on the fast entry lock too !
In all cases, if you use a rotary vane pump, put a foreline trap on it.
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess 67037 Strasbourg CEDEX France
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Does anyone out there have any experience to share } about the Philips XL-30 (Sirion or other) with EDAX relative } to potential consequences of a diffusion pump versus } a turbo? It seems to me that a turbo pump would } go a long way to prevent contamination of the } detector window. I am not sure about this. The } Sirion isolates the chamber from the diffusion pump } during specimen exchange. Plus, it has a chilled } water trap. So this may not be a problem. } } gg } }
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, } } We recently aquired a JSM5600LV SEM, with a Peltier cold stage. What is the } purpose of using this decive? All info on this is welcome! } } Thanks } } Renaat Dasseville } Protistology & Aquatic Ecology } Dept. Biology, University Gent } Krijgslaan 281, S8 } 9000 Gent, B E L G I U M } } tel: +32 9 264 85 04 } fax +32 9 264 85 99 } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
We are trying to conduct immunofluorescent labeling of tissue arrays of paraffin sections of multiple tissue types (from Abcam).....we are having problems reducing the background fluorescence in some tissues....liver and lung to name just a few. These are blaring green with FITC secondary antibodies alone! Would anyone have good (low background noise) protocols for labeling these difficult tissue types?
I thank you in advance for any advice we can get on this. Sophie
Sophie Dahan, Ph.D. Senior Scientist, Microscopy Lab Caprion Pharmaceuticals, Inc. Montreal, Quebec Canada
Microscopy and Digital Imaging: Advances and Applications. An afternoon with Dr. Alan Hibbs, Biocon, Ltd., Melbourne, Australia Thursday, August 1, 2002, Health Sciences Building Room G-417
1:30-2:15 Medical diagnosis using miniaturized confocal microscopes and a review of current confocal instruments for research.
2:30-3:15 Culture on the stage - growing cells and tissues on the microscope for live cell imaging.
3:30-4:30 Refreshments and a roundtable discussion of audience questions regarding microscopy, confocal microscopy, live cell imaging or related issues: with the audience, Dr. Hibbs, invited UW faculty and staff.
Dr. Hibbs provides training and consulting throughout the world for confocal microscopy and live cell microscopy.
Presented by: The University of Washington Core for Communication Research - V.M. Bloedel Hearing Research Center; Cellular Imaging Facility - Center for Human Development and Disability; W.M. Keck Center for Advanced Studies in Neural Signaling.
Refreshments are provided courtesy of Intelligent Imaging Innovations, Denver, CO www.intelligent-imaging.com
Please contact Glen MacDonald for more information. -- Glen MacDonald Microscopy and Imaging Facility University of Washington Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 glenmac-at-u.washington.edu (206) 616-4156 (206) 616-1828 fax ************************************************************************** C:} The box said "Requires Windows95 or better". So I bought a Macintosh. **************************************************************************
Many thanks for the many very creative approaches to preparing minute quantities of sperm for TEM. I'll pass them along to the investigator and give some of them a try.
Dotty
Dotty Sorenson Microscopy and Image Analysis Laboratory Department of Cell and Developmental Biology University of Michigan Medical School Ann Arbor, Michigan (734)763-1170 FAX (734)763-1166 dsoren-at-umich.edu
Dear TEM Community, We have a Jeol 100C 100 KV TEM serial # EM156009-05, that we are willing to donate to a nonprofit or educational institution. The instrument was built in 1974 and is in fair condition. Scope has 3 sets of 50 plate film cassettes and boxes with built in vacuum film desiccator. It has been under service contract with Jeol for the last 10 years. We will be dismantling the scope at the end of this month It will be the donee's responsibility to come and take the microscope. Interested parties should send statement of purpose and reasons why we should select them as the donee for this microscope. Please contact
Joe Goodhouse Confocal / EM Core Laboratory Manager Department of Molecular Biology Princeton University jgoodhouse-at-molbio.princeton.edu 609-258-5432
Joe Goodhouse Confocal / EM Core Laboratory Department of Molecular Biology Princeton University 609-258-5432
Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
Try contacting Acton Technologies in Pennsylvania, USA, "www.actontech.com." I didn't see an etch specfically for Ni-Ti but they may offer some solutions. They make up custom mixtures as well as off-the-shelf products.
Peter Tomic Anadigics, Inc.
-----Original Message----- } From: cf_reu-at-ccmr.cornell.edu [mailto:cf_reu-at-ccmr.cornell.edu] Sent: Friday, July 19, 2002 9:49 AM To: microscopy-at-sparc5.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cf_reu-at-ccmr.cornell.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday, July 18, 2002 at 10:00:16 ---------------------------------------------------------------------------
Email: cf_reu-at-ccmr.cornell.edu Name: Chris Fanning
Organization: Cornell University
Education: Undergraduate College
Location: Ithaca, Ny, US
Question: I am involved in undergraduate materials research on Nickel-Titanium (50.5% ni). I have prepared an ~1cm cube of nitinol potted in epoxy to be viewed in an SEM. Our goal is to obtain an ODF via an EBSD scan. to accomplish this we need to get well defined patterns in the EBSD. We are getting patterns now but they are not good enough. We are considering etching and need recomendations for etching solutions. Many people suggest an HF solution. Are there alternatives to HF?
Our material prep was, ... -cut with a slow speed (200 rpm) precision saw, diamond blade -rough polish with 240, 320, 480(?), 600, 1200 Si-carbide paper by hand -fine polish with 9u, 3u, 1u on nylon paper - polishing wheel, 200rpm -fine polish with colloidal silica, by hand -4 hrs on vibraory polisher with colloidal silica
any advice would be much appreciated! sorry for the long email,
} } Email: dpu-at-spdc.ti.com } Name: Dennis Pu } } Organization: Texas Instruments } } Education: Graduate College } } Location: Dallas, Texas 75243 } } Question: Hello, } This is not exactly a question for school, } but I hope we're all here to learn and explore. } My question deals with using microscopy to view } detailed images of the inside of vinyl record } grooves. My desire is to research what it might } take to build an optical record player. } I'm trying to determine whether it is } more practical to utilize some kind of optical } tracking of the groove for real-time play or } if scanning the whole record in at once and using } software to recognize the grooves and to produce } the sound would be better. } My first step is just to determine ways I } can use microscopy to even see inside groove. I'd } consider one economical option that hobbyists } could tinker with at home, and one "best" solution } archivists can use for transcribing old media } (vinyl, wax cylinders, acetates, etc...) without } having to use destructive mechanical means. } I'd appreciate any suggestions you have } to offer. } } Thanks, } Dennis Pu } } --------------------------------------------------------------------------- } Dear Dennis, One problem you'll have is that vinyl doesn't reflect very well, so it will be hard to use optical microscopy to see the groove shape. Also, the contrast will be inherently low. Trying to get the 3-D shape of both sides of the groove will be a lot harder than determining this mechanically with a macroscopic version of the atomic-force microscope--aka stylus and cartridge. It might be possible to coat the grooves with a very thin layer of a reflective metal and get a stereo view; a CD reader must do something like this, but the information is probably stored in a different manner to facilitate optical reading. Good luck. Yours, Bill Tivol
I collect and restore wind up phonographs. I remember reading about a project where someone scanned Edison wax cylinder records with a laser(s) and created recordings that contained more sounds than could be heard through playing the records on the original phonographs. The information got recorded by the original mechanical means, but could not be heard through mechanical playing. They may have done it with early 78's, also, but I can't remember. It was a long time ago. FYI, the cylinder records are "vertically cut", as opposed to "horizontally cut" like the 78's and the later vinyl records.
Anyway, you might try searching for the information under phonos, records, etc.
Regards, Darrell
dpu-at-spdc.ti.com (by way of Nestor J. Zaluzec) on 07/19/2002 09:50:36 AM
To: microscopy-at-sparc5.microscopy.com cc:
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dpu-at-spdc.ti.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on Thursday, July 18, 2002 at 13:16:32 ---------------------------------------------------------------------------
Email: dpu-at-spdc.ti.com Name: Dennis Pu
Organization: Texas Instruments
Education: Graduate College
Location: Dallas, Texas 75243
Question: Hello, This is not exactly a question for school, but I hope we're all here to learn and explore. My question deals with using microscopy to view detailed images of the inside of vinyl record grooves. My desire is to research what it might take to build an optical record player. I'm trying to determine whether it is more practical to utilize some kind of optical tracking of the groove for real-time play or if scanning the whole record in at once and using software to recognize the grooves and to produce the sound would be better. My first step is just to determine ways I can use microscopy to even see inside groove. I'd consider one economical option that hobbyists could tinker with at home, and one "best" solution archivists can use for transcribing old media (vinyl, wax cylinders, acetates, etc...) without having to use destructive mechanical means. I'd appreciate any suggestions you have to offer.
An interesting assignment. However, you should be aware that the grooves are an analog of the frequency and magnitude of the sounds recorded. If you image the grooves of a record, say on an electron microscope, you will notice that it not only has waves horizontally, but waves in the "Z" axis as well [vertically]. The two surfaces represent the left and right channels of a stereo recording, and are thus separated.
Your assignment seems to deal with a non-destructive method of getting that analog data off the surface by some means of "imaging." Electron microscopy, it seems to me, may image a small area, but generating "numbers" from that surface will be daunting. And it's numbers you need, not another analog representation. You may look into "ultrasonic" imaging, at least for the Z axis, something that works with "Time-of-flight" like radar, but acoustically. I have an instrument in the laboratory called a "Sonoscan" made by Sonoscan Corp. that can generate images of surfaces non-destructively. There is also AFM, [Atomic Force Microscope] but the scales you need are much more gross. You don't need to measure angstroms, but probably 10's of microns. One pass of a record needle on that vinyl and you've just milled off many angstroms. One question you will need to answer is what the dynamic range of those grooves are from peak to trough so that you can faithfully reconstruct the sound range in magnitude and the other question is the rate of change in the grooves per linear measure to reconstruct the frequency or pitch of the sound.
If you'd like, I would be more than happy to take a piece of vinyl record and image it in our SEM to give you some notion of the surface you are dealing with. However, you may have to supply the record, or at least a piece of it. My wife will not like me sawing up her Frank Sinatra records for such lofty scientific endeavors, even if it's for a student.
Good luck and publish your results.
Peter Tomic Anadigics, Inc.
-----Original Message----- } From: dpu-at-spdc.ti.com [mailto:dpu-at-spdc.ti.com] Sent: Friday, July 19, 2002 9:51 AM To: microscopy-at-sparc5.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dpu-at-spdc.ti.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on Thursday, July 18, 2002 at 13:16:32 ---------------------------------------------------------------------------
Email: dpu-at-spdc.ti.com Name: Dennis Pu
Organization: Texas Instruments
Education: Graduate College
Location: Dallas, Texas 75243
Question: Hello, This is not exactly a question for school, but I hope we're all here to learn and explore. My question deals with using microscopy to view detailed images of the inside of vinyl record grooves. My desire is to research what it might take to build an optical record player. I'm trying to determine whether it is more practical to utilize some kind of optical tracking of the groove for real-time play or if scanning the whole record in at once and using software to recognize the grooves and to produce the sound would be better. My first step is just to determine ways I can use microscopy to even see inside groove. I'd consider one economical option that hobbyists could tinker with at home, and one "best" solution archivists can use for transcribing old media (vinyl, wax cylinders, acetates, etc...) without having to use destructive mechanical means. I'd appreciate any suggestions you have to offer.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (drands-at-avuhsd.k12.ca.us) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July 19, 2002 at 22:18:04 ---------------------------------------------------------------------------
Email: drands-at-avuhsd.k12.ca.us Name: Dave Rands
Organization: Lancaster High School/Special Education Department
Education: 9-12th Grade High School
Location: Lancaster, California, Los Angeles County
Question: We are having a difficult time getting our paraffin wax to fill in around our speciman. We have tried several things and have asked the other science teachers with no success. Can you help?
Hello everyone, I read the item about a hand-held system for GFP emission. Our lab has been using a number of lighting systems for GFP, YFP and RFP that are manufactured in Hungary by BLS ( http://www.bls-ltd.com/ ). Some results can be seen at ( http://www.mshri.on.ca/nagy ).Mr. Lajos Laszlo has many years of experience in the development of biological equipment. Regards, Stephen Black
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (nuruh-at-uccmail.co.tz) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July 19, 2002 at 09:54:16 ---------------------------------------------------------------------------
Email: nuruh-at-uccmail.co.tz Name: Saleh R
Organization: university of dar es salaam
Education: Graduate College
Location: Dar es salaam, Tanzania
Question: I want to index some spots of an electron diffraction patterns, which web sites and/or books can help me on this?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bjbecker-at-ucsd.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July 19, 2002 at 20:01:15 ---------------------------------------------------------------------------
Email: bjbecker-at-ucsd.edu Name: Bonnie Becker
Organization: Scripps Institution of Oceanography
Education: Graduate College
Location: San Diego, CA USA
Question: I work with larval mussels (on the order of 100 microns), using laser ablation technology to analyze their chemical structures. I would like to stick them on a microscope slide such that
1. They don't pop off 2. I can put them on the slide in water and let the water dissolve without handling them individually. 3. If possible (doubt it), it should be trace metal free.
So, I am looking for a coated microscope slide, but I don't know if I need poly-l-lysine, silane, or something else? The shells are CaCO3, and they are on the order of 100 microns.
Schroedinger's cat aside, some ethical and legal issues about scientific images will be raised at M&M 2002 in Quebec.
First, at the Presidential Happenings symposium on Monday at 5:00, Colin Smith of Adobe Systems, Inc. in Canada will present "Harnessing the Power of Photoshop 7", demonstrating the new and powerful features of this latest release of Adobe Photoshop. Those of you who saw Julianne Kost's excellent presentation at M&M 2001 in Long Beach will remember the audience gasping at both the wonderful features of Photoshop 6 they may not have known about, and also at the power to potentially manipulate image data beyond all recognition.
On Tuesday the Problem Solving with the Experts program, beginning at 8:00 am, will be Addressing Issues in Digital Imaging for the Microscopist: II. In addition to Jose Mascorro speaking about converting film negatives to digital files and Judy Murphy explaining her solution for handling huge numbers of digital files in the microscopy laboratory, I will be presenting a talk, "Adobe Photoshop for Image Adjustment: How to Start and When to Stop". For the first part I will demonstrate the usual steps required to prepare digital micrographs and combine them into a figure plate for publication. Then I hope to stimulate a discussion on the kinds of manipulation that can be performed on images and the effects those may have on data, and what this might mean for truth and ethics in scientific imaging.
On Thursday, beginning at 10:30 am, the Technologists' Forum Roundtable Discussion will be about the Legal and Ethical Issues of Data Ownership, focusing on copyrights of images and other forms of data. Barbara M. Knoppers, a recognized expert in ethics and the law, and other representatives of academia and industry will be on the panel.
In addition, there may also be a Public Policy Committee session on Wednesday at 2:00 pm about the copyright and legal issues, so watch the program and daily newsletter for an announcement.
The MSA Education Committee has recently added a subcommittee on the Ethics of Digital Image Processing, with the purpose of drafting a white paper of recommendations.
As you can see, there are lots of opportunities for lively discussion!
See you all in Quebec, Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Listers, Here finally are the results of the facility support survey first put out at last year's M&M meeting, then on the microscopy and confocal listservers. Alltheusualexcuses for the delay. Including formatting the results in ascii for the email servers. I did try to think of wise things to say about this, but any comments would take far too much space for the listservers, so I've decided to simply present the raw numbers. I hope there will be room to run these results in Microscopy Today as graphs or something, but I can make no promises. %s of course don't add to 100 because of rounding errors.
The analyses of the returned surveys were done by Barbara Foster's crew MME ( mme-at-mail.map.com ). Many thanks, Barbara and gang.
There is no heading #1 as that contained information that would identify individual institutions. I discarded that information before I sent the surveys to MME or indeed did anything else. This information was collected solely in case of duplicate responses, and was trashed as soon as it was no longer needed for this purpose.
There is the usual problem with sample size. I hope the information will be useful in spite of this. I also hope some vestige of legible organization survives the email process ... Phil
2. Nature of institution COUNT % Academic 50 89 Other 4 7 Private research 2 4 Commercial 1 2 56 Total Respondents 57 Total Responses
3. Type of facility COUNT % Institution core 32 58 Departmental 19 35 Other 5 9 Satellite 1 2 55 Total Respondents 57 Total Responses 4. Predominate work COUNT % Biological 53 87 Materials 27 44 61 Total Respondents 80 Total Responses
5. User Base COUNT % Multi - user 56 93 Service 47 78 60 Total Respondents 103 Total Responses
6. Type of microscopes COUNT % TEM 48 80 SEM 36 60 Optical 18 30 Confocal 18 30 Other 18 30 Other Optical 16 27 FESEM 12 20 ESEM or LV 12 20 Other Confocal 5 8 Scanning Probe 5 8 FETEM 3 5 Other Scanning Probe 3 5 60 Total Respondents 194 Total Responses
7. Salaries COUNT % 100% Your Institution 31 50 80% Your Institution 6 10 50% Your Institution 5 8 100% User fees 4 6 20% Grants 4 6 100% Grants 4 6 10% Your Institution 3 5 20% User fees 3 5 25% User fees 3 5 30% User fees 3 5 50% User fees 3 5 25% Grants 3 5 70% Your Institution 2 3 75% Your Institution 2 3 10% User fees 2 3 10% Grants 2 3 50% Grants 2 3 Other 2 3 20% Your Institution 1 2 30% Your Institution 1 2 60% Your Institution 1 2 90% Your Institution 1 2 Other 1 2 40% User fees 1 2 60% User fees 1 2 80% User fees 1 2 Other User fee percentage 1 2 30% Grants 1 2 40% Grants 1 2 70% Grants 1 2 75% Grants 1 2 Other Grants 1 2 62 Total Respondents 98 Total Responses
8. Instrument maintenance COUNT % 100% User fees 19 31 100% Your Institution 18 30 50% Your Institution 6 9 50% Grants 4 6 10% Your Institution 3 5 90% Your Institution 3 5 50% User fees 3 5 60% User fees 3 5 20% Your Institution 2 3 40% Your Institution 2 3 Other 2 3 10% User fees 2 3 80% User fees 2 3 90% User fees 2 3 10% Grants 2 3 20% Grants 2 3 100% Grants 2 3 25% Your Institution 1 2 30% Your Institution 1 2 25% User fees 1 2 30% User fees 1 2 60% Grants 1 2 75% Grants 1 2 Other 1 2 61 Total Respondents 84 Total Responses
9. Replacement of existing equipment COUNT % 100% Grants 16 30 100% Your Institution 11 20 50% Your Institution 6 11 50% Grants 5 9 60% Grants 5 9 20% Your Institution 4 7 30% Your Institution 4 7 10% User fees 4 7 100% User fees 4 7 25% Your Institution 3 5 40% Your Institution 3 5 20% User fees 3 5 50% User fees 3 5 10% Your Institution 2 4 70% Grants 2 4 75% Grants 2 4 90% Grants 2 4 40% User fees 1 2 75% User fees 1 2 80% User fees 1 2 90% User fees 1 2 10% Grants 1 2 40% Grants 1 2 80% Grants 1 2 Other 1 2 56 Total Respondents 87 Total Responses
10. Purchasing of new equipment COUNT % 100% Grants 21 37 100% Your Institution 9 16 50% Your Institution 6 11 50% Grants 6 11 20% Your Institution 5 9 30% Your Institution 5 9 70% Grants 4 7 20% User fees 3 5 60% Grants 3 5 80% Grants 3 5 10% Your Institution 2 4 25% Your Institution 2 4 40% Your Institution 2 4 75% Your Institution 2 4 10% User fees 2 4 30% User fees 2 4 100% User fees 2 4 10% Grants 2 4 90% Grants 2 4 60% Your Institution 1 2 70% Your Institution 1 2 40% User fees 1 2 25% Grants 1 2 40% Grants 1 2 75% Grants 1 2 57 Total Respondents 89 Total Responses
11. Supplies COUNT % 100% User fees 28 46 100% Your Institution 9 15 50% Grants 5 8 80% Your Institution 4 7 10% Your Institution 3 5 50% Your Institution 3 5 50% User fees 3 5 90% User fees 3 5 10% Grants 3 5 90% Your Institution 2 3 10% User fees 2 3 30% User fees 2 3 20% Grants 2 3 100% Grants 2 3 20% Your Institution 1 2 25% Your Institution 1 2 30% Your Institution 1 2 40% Your Institution 1 2 60% Your Institution 1 2 20% User fees 1 2 25% User fees 1 2 40% User fees 1 2 60% User fees 1 2 80% User fees 1 2 40% Grants 1 2 70% Grants 1 2 90% Grants 1 2 Other 1 2 61 Total Respondents 85 Total Responses
12. Operating expenses COUNT % 100% User fees 19 40 100% Your Institution 16 33 50% Your Institution 6 12 50% User fees 6 12 100% Grants 3 6 20% Your Institution 1 2 40% Your Institution 1 2 60% Your Institution 1 2 80% Your Institution 1 2 40% User fees 1 2 60% User fees 1 2 80% User fees 1 2 20% Grants 1 2 Other 1 2 48 Total Respondents 59 Total Responses -- }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{ Philip Oshel Supervisor, AMFSC and BBPIC microscopy facilities Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax)
Will the transactions at the conference be available either electronically or via print? This would be most interesting.
I'm curious about the distinction between ethics and legal issues. Image analysis technology can produce legal data in cases where it was not possible in the past. I don't see that this has anything to do with ethics. It seems to me to be an issue of what can technology do and then what will the legal system accept? There are numerous instances of this issue. So far as I know, technology won.
when I saw your query, I thought I had seen such a technology mentioned in a magazine. If you look at the websites below there are companies which are already using laser scanning technology to read vinyl LPs.
http://www.stereotimes.com/turn030300.shtm
http://avconvert.com/laserturntable/
This would suggest that this is a mature technology but the 'kit' is very expensive.
Malcolm Haswell
"by way of Nestor J. Zaluzec" wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (dpu-at-spdc.ti.com) from } http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on } Thursday, July 18, 2002 at 13:16:32 } --------------------------------------------------------------------------- } } Email: dpu-at-spdc.ti.com } Name: Dennis Pu } } Organization: Texas Instruments } } Education: Graduate College } } Location: Dallas, Texas 75243 } } Question: Hello, } This is not exactly a question for school, } but I hope we're all here to learn and explore. } My question deals with using microscopy to view } detailed images of the inside of vinyl record } grooves. My desire is to research what it might } take to build an optical record player. } I'm trying to determine whether it is } more practical to utilize some kind of optical } tracking of the groove for real-time play or } if scanning the whole record in at once and using } software to recognize the grooves and to produce } the sound would be better. } My first step is just to determine ways I } can use microscopy to even see inside groove. I'd } consider one economical option that hobbyists } could tinker with at home, and one "best" solution } archivists can use for transcribing old media } (vinyl, wax cylinders, acetates, etc...) without } having to use destructive mechanical means. } I'd appreciate any suggestions you have } to offer. } } Thanks, } Dennis Pu
What kind of specimens are you working with? I'm not sure what you meant by " {/color} We are having a difficult time getting our paraffin wax to fill in around our speciman" - do you mean the wax won't literally surround your specimen, or do you mean the wax won't go {italic} inside {/italic} the specimen? If you mean it won't go inside the specimen, you can try placing your specimen, together with the wax, under a vacuum overnight. If this still doesn't work.... maybe your tissue isn't fixed properly?
Hope this helps! {color} {param} 0100,0100,0100 {/param}
{nofill}
"When life hands you a lemon ... bring out the tequila and salt."
The liver is a biochemical factory with one of its functions focused on detoxification. It's association with degradation of hemoglobin is the source of your consternation. If you want to defeat the autofluorescence, you will have to post-process the molecules to "quench" them chemically without destroying any of the cellular antigens. Since the source of this background is most likely large quantities of resonant molecules, I would think a bath in bromine (additive) might do the trick, or a hydrogenation (additive too but perhaps more dangerous), or a hydration, but the last is usually hydrolytic. This starts as a problem in organic chemistry and ends with a choice of a relatively mild alternative.
The folks at Yale seem to have some experience here, you might ask them directly for help on the above.
I think!
Good luck,
Fred Monson
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center West Chester University South Church Street and Rosedale West Chester, Pennsylvania, USA, 19383 Phone: 610-738-0437 FAX: 610-738-0437 fmonson-at-wcupa.edu CASI URL: http://darwin.wcupa.edu/casi/ WCUPA URL: http://www.wcupa.edu/ Visitors URL: http://www.wcupa.edu/_visitors/
} ---------- } From: Sophie Dahan } Sent: Friday, July 19, 2002 12:35 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: Immunofluorescence labeling of liver and lung paraffin } sections } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, } } We are trying to conduct immunofluorescent labeling of tissue arrays of } paraffin sections of multiple tissue types (from Abcam).....we are having } problems reducing the background fluorescence in some tissues....liver and } lung to name just a few. These are blaring green with FITC secondary } antibodies alone! } Would anyone have good (low background noise) protocols for labeling these } difficult tissue types? } } I thank you in advance for any advice we can get on this. } Sophie } } Sophie Dahan, Ph.D. } Senior Scientist, Microscopy Lab } Caprion Pharmaceuticals, Inc. } Montreal, Quebec } Canada } } }
Here's the REAL dope, answering your question with a photo and a URL.
Hope it helps: http://members01.chello.se/christer.hamp/phono/poliak.html
Regards,
Fred Monson
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center West Chester University South Church Street and Rosedale West Chester, Pennsylvania, USA, 19383 Phone: 610-738-0437 FAX: 610-738-0437 fmonson-at-wcupa.edu CASI URL: http://darwin.wcupa.edu/casi/ WCUPA URL: http://www.wcupa.edu/ Visitors URL: http://www.wcupa.edu/_visitors/
} ---------- } From: dpu-at-spdc.ti.com } Sent: Friday, July 19, 2002 9:50 AM } To: microscopy-at-sparc5.microscopy.com } Subject: Ask-A-Microscopist: Imaging Grooves in Records } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (dpu-at-spdc.ti.com) from } http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on } Thursday, July 18, 2002 at 13:16:32 } -------------------------------------------------------------------------- } - } } Email: dpu-at-spdc.ti.com } Name: Dennis Pu } } Organization: Texas Instruments } } Education: Graduate College } } Location: Dallas, Texas 75243 } } Question: Hello, } This is not exactly a question for school, } but I hope we're all here to learn and explore. } My question deals with using microscopy to view } detailed images of the inside of vinyl record } grooves. My desire is to research what it might } take to build an optical record player. } I'm trying to determine whether it is } more practical to utilize some kind of optical } tracking of the groove for real-time play or } if scanning the whole record in at once and using } software to recognize the grooves and to produce } the sound would be better. } My first step is just to determine ways I } can use microscopy to even see inside groove. I'd } consider one economical option that hobbyists } could tinker with at home, and one "best" solution } archivists can use for transcribing old media } (vinyl, wax cylinders, acetates, etc...) without } having to use destructive mechanical means. } I'd appreciate any suggestions you have } to offer. } } Thanks, } Dennis Pu } } -------------------------------------------------------------------------- } - } }
This is not a technical problem. I am seaching for graphics software for creating\printing posters and banners. We have poster sessions several times a year for the students. We were using an old DOS program but our computer lab has been upgraded(OS + network printers) and I can't get the program to work with the network printers.
Does anyone have any suggestions for this software application? It has to be user friendly because of the students. And it has to be cheap because of the budget.
Thanks,
Steve Widing EM Tech / Computer Labs Manager Biology Department Temple University Philadelphia, PA
Course Announcement for Microscopy Workshop, west coast Deadline to enroll August 7, 2002
The seminar / workshop will be an intensive lecture/laboratory series that will enable participants to develop theoretical and hands-on expertise with light microscopes. Attendees will closely interact with the instructors while using modern research grade microscopes, cameras, and computers.
The Integrated Microscopy Facility, operated by the Department of Molecular, Cellular, and Developmental Biology (MCDB) and the Neuroscience Research Institute (NRI), is offering a workshop on modern techniques in microscopy and electronic imaging. This 5-day workshop will be offered from September 9th thro