A post-doctoral research associate position is available within the Center for High Resolution Electron Microscopy at Arizona State University. The position is sponsored by a consortium of semiconductor companies and the primary focus of the research will be the development and application of electron holography to industrially relevant semiconductor devices. Areas of particular interest include the study of lateral dopant diffusion and the effect of thermal annealing treatment on dopant diffusion. Candidate must have a Ph.D. in material science, or physics, with experience in characterization of electronic devices using electron holography. Experience with the techniques of high resolution imaging, and electron energy loss spectroscopy is preferred. Please submit your resume together and the names and addresses of 3 referees to: Professor David J. Smith, Director, Center for Solid State Science, Arizona State University, Tempe, AZ 85287-1704, Fax (480) 965-9004, email csss.director-at-asu.edu. AA/EOE
John C. Wheatley Lab Manager Arizona State University Center for Solid State Science PSA-213 BOX 871704 Tempe, AZ 85287-1704
We recently did an environmental health & safety assessment looking at this, although we were more concerned with the very toxic hydrides. None were detected with a standard portable gas monitor (the type which clips on a belt, used when changing As cylinders on MOVPE kits etc.) 3 inches above the griding paper (1200 grit SiC, grinding about 1 cm^2 of GaAs at ~500 rpm in continuous running water). I've never noticed a smell when grinding GaAs (and I've been doing it for ten years!). InP is another story - a very strong smell is noticable, and it was decided that fume extraction was a good idea (a lot easier than holding your breath!). The dose without ventilation was within UK occupation health limits as long as it was done for less than 30 minutes/day. (This only applies to relatively coarse grinding, where you're removing a lot of material. Polishing is okay.) As Mike says, why take chances when there are solutions available.
Richard
____________________________________________________________________________ ______ Mike Bode said:
I would definitely recommend some form of ventilation for all cutting and polishing operations. When we dimpled GaAs samples for TEMs, it was sometimes possible to smell the typical Garlic odor from the Arsenicoxide (and no, I had not been out eating Garlic soup the day before). Although I don't know at this time if Arsenicoxide is toxic and at what levels (not too low, as I am still writing this), why take the chances.
mike
} } } } } } } } } } WE HAVE MOVED { { { { { { { { { please make a note of the new address below
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Mary Mager [mailto:mager-at-interchange.ubc.ca] Sent: Tuesday, July 30, 2002 12:23 PM To: Armando Verdugo Cc: Microscopy-at-sparc5.microscopy.com
Dear Armando, When I had to organize the cleanup of a crystal-growing room that had left a lot of GaAs dust around, I looked up the MSDS in order to devise a cleanup protocol. The arsenic is only a problem if the material is acidified, so we mopped up with a little soap and water and discarded the wet paper towels and protective clothing as comtaminated waste. GaAs is fairly inert, but I would avoid allowing any dust out of your cutting or polishing operations. Clean up any fragments or dust and treat as any other arsenic-containing compound: avoid inhalation and skin contact. At 11:47 AM 07/29/2002 -0700, you wrote:
} Hello listers, } } I need to gather information on the toxicity and possible health risks that } may be encountered by my technicians in processing (i.e. Cross-Sectioning, } TEM/Pre-FIB, Backside Grinding, etc.) GaAs die sections in our applications } laboratory. } } Any policies from other labs would be appreciated, as well as points of } reference. } } Thank you all in advance. } } Regards, } } Armando Verdugo Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
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Dear Group - what is the preferred method to apply colloidial gold (40 nm) to silicon monoxide on formvar 200 mesh Cu grids? Right now I would like to use the colloidial gold as a reference when imaging nanoparticles. I have never used this colloidial gold before, so I was surprised to see it in a liquid form. Thanks Barbara
ok, as fred knows, i hate solving other peoples preoblems for them. i have enough of my own. here goes: the fault is in one or more of the following areas: either to short a time in OsO4, that is the membranes were not stabilized. to long a time in OsO4, it is rarely explained to newcomers, but OsO4 can leach out cellural componets. no more than 1 hour with no more than 2% OsO4.
to long a dehydration in ethanol assuming that is what you are using. no more than 10 minutes per dehydration step. for cells i always start at eith 50 ro 70%. always use a 2 15 min propylene oxide as an intermediate step. finaly what is your embedding media? you sould be using an epon subsitute.
in all my 20+ years of doing EM i have never lost a single membrane. tissue culture cells can be picky.
fred is correct, you need to take control of all your solutions. no one i repeat no should be using the solutions you are using. that is asking for trouble. always mix your own fixatives and embedding media, unless it is someone you trust completely.
you should send us you embedding schdule. it will make figuring out whats going on a lot simpler. ok my carpel tunnel is acting up so enough typing. good luck EM really isn't hard, it's just 100 times more excating that histo. John Hoffpauir
PS: fred we are on the final plans for our scope room. hows yours going?
With a heart full of tears I write to seek for your help at this time of grief in my family. I am the wife of Nigeria's former head of state, Late Gen. Sani Abacha [Alhaja Mariam Abacha].
Soon after the demise of my husband, the succeeding regime of Abdusallami Abubakar besieged my family and started probing every activity of my husband while in office. The present civilian government continued with probe to the extent of arresting my son Mohammed Abacha alongside with my husband's Chief Security Officer (CSO), major Hamza Mustapha. The two are currently facing trial in the court.
Several false allegation of murder of some politician, Activists/prodemocracy agents; misgovernance and vast loot of the Nations Treasury were leveled against my husband. As a result, all our properties have been seized and our Bank Account frozen leaving us with no means of li! velihood.
The only money left for us is a defaced cash sum of US$44M(Forty-Four Million U.S Dollars) contained In trunk boxes which I instructed my half brother to take out of the country immediately my husband died. The money has to be defaced in order to beat security operatives while on transit. My half brother was able to cross the box to Europe and deposited them with a Trust / Security Companys family Treasures before His death.
Right now, my family is in urgent need of having this money invested outside Nigeria because of several ongoing probes on ex-service men and also political instability in the country.
My request therefore is for you to give me necessary assistance to claim this money from the security company and transfer it to your personal account in your country. Our family lawyer will process the enabling documents in your name as the Trustee/Beneficiary of the fund to facilitate its withdrawal.
I am equally willing to compensat! e your effort with 30% of the money when it arrives your account.
Please note that government is still keeping surveillance over the activities of my family with regards to traveling and Telephone calls. Therefore you should treat this business with absolute confidentiality. All your correspondence to me must be strictly through my e-mail address.
The lawyer as my representative would be meeting you in due course on my behalf as the need may arise. Please contact him on his mobile phone number: 234-803-327-9244
I assure you that no risk of any kind is involved in this transaction.
With a heart full of tears I write to seek for your help at this time of grief in my family. I am the wife of Nigeria's former head of state, Late Gen. Sani Abacha [Alhaja Mariam Abacha].
Soon after the demise of my husband, the succeeding regime of Abdusallami Abubakar besieged my family and started probing every activity of my husband while in office. The present civilian government continued with probe to the extent of arresting my son Mohammed Abacha alongside with my husband's Chief Security Officer (CSO), major Hamza Mustapha. The two are currently facing trial in the court.
Several false allegation of murder of some politician, Activists/prodemocracy agents; misgovernance and vast loot of the Nations Treasury were leveled against my husband. As a result, all our properties have been seized and our Bank Account frozen leaving us with no means of li! velihood.
The only money left for us is a defaced cash sum of US$44M(Forty-Four Million U.S Dollars) contained In trunk boxes which I instructed my half brother to take out of the country immediately my husband died. The money has to be defaced in order to beat security operatives while on transit. My half brother was able to cross the box to Europe and deposited them with a Trust / Security Companys family Treasures before His death.
Right now, my family is in urgent need of having this money invested outside Nigeria because of several ongoing probes on ex-service men and also political instability in the country.
My request therefore is for you to give me necessary assistance to claim this money from the security company and transfer it to your personal account in your country. Our family lawyer will process the enabling documents in your name as the Trustee/Beneficiary of the fund to facilitate its withdrawal.
I am equally willing to compensat! e your effort with 30% of the money when it arrives your account.
Please note that government is still keeping surveillance over the activities of my family with regards to traveling and Telephone calls. Therefore you should treat this business with absolute confidentiality. All your correspondence to me must be strictly through my e-mail address.
The lawyer as my representative would be meeting you in due course on my behalf as the need may arise. Please contact him on his mobile phone number: 234-803-327-9244
I assure you that no risk of any kind is involved in this transaction.
Electron Microscopy Laboratory Manager at Purdue University
An opening is now available for an energetic individual to manage a multi-user electron microscopy facility in the Department of Biological Sciences at Purdue University. The successful candidate will have primary responsibility for the day-to-day operations of a laboratory specializing in transmission electron cryomicroscopy of biological macromolecules and their assemblies. Responsibilities include:
Overseeing the routine maintenance and use of a multi-user high-resolution transmission electron microscopy facility
Training new users in use of the microscopes and in specimen preparation techniques for high-resolution imaging of biological macromolecules
Evaluating equipment performance regularly to maintain maximum performance standards
Working with faculty, students, and visiting scientists on scientific experiments as time permits
Associates or higher degree and a minimum of 1 year of experience working in and/or managing a transmission electron microscopy laboratory required. Excellent written and oral communication skills are essential. Computer skills are required. Successful candidate will be trained in preparation and imaging techniques and necessary diagnostic skills for high-resolution electron cryomicroscopy.
Purdue University is home to outstanding researchers in the biological sciences as well as a world-reknown group of structural biologists. The Department of Biological Sciences provides a stimulating intellectual environment as well as outstanding modern scientific facilities including FEI CM300-FEG, CM200-FEG, and EM420 electron cryomicroscopes dedicated to structural studies and an EM410 for routine biological work. Purdue is home to over 50,000 students, faculty, and staff, and is located in West Lafayette, Indiana -- approximately 1 hour northwest of Indianapolis and 2 hours southeast of Chicago. The area provides the advantages associated with a major university while providing an affordable and attractive small town environment in which to live.
Interested individuals should contact: Prof. Amy McGough Department of Biological Sciences Purdue University West Lafayette, IN 47907-1392 USA fax: +1 (765) 496-1189
Purdue is an equal access/equal opportunity university
The Microscopy Group in the Analytical and Computational Technology Center of the Rohm and Haas Company, one of the world's largest specialty chemical companies, is seeking candidates for a senior scientist position. The position is located at our Spring House site, near Philadelphia. The senior scientist will use electron microscopy and related technologies included in our microscopy lab (SEM/EDS, TEM, Optical, AFM, etc.) to study materials from across the company including various polymers, coatings, colloids, catalysts, electronics materials, and other complex systems. The senior scientist will develop test methods, and explore new technologies or combinations of technologies to address issues in our product research departments. The senior scientist will often work in collaboration with other analytical and product area scientists and have responsibility for technical oversight of more routine work done by other researchers.
Applicants for this position must have a Ph.D. degree in Chemistry, Chemical Engineering, Materials Science, or a closely related discipline, with substantial experience in electron microscopy. Broader experience in other experimental techniques for materials characterization is desirable. Experience and theoretical knowledge in any of the following areas is desirable: polymer science, colloid science, catalysis, XRF, Surface Analysis, crystallography, and computer programming. The applicant must have good communication skills, strong initiative, and be able to succeed in a collaborative environment.
Rohm and Haas offers a highly competitive total compensation program involving base salary calibrated against the market and variable pay opportunity through an annual bonus program. Relocation assistance will be provided. We are committed to the professional development of our Technology staff. Candidates for this position should forward their resumes to: Dr. John R. Reffner, Rohm and Haas Company, 727 Norristown Road, P.O. Box 904, Spring House, PA 19477 or by fax to (215) 619-1607.
Rohm and Haas is an Equal Opportunity Employer.
NOTE: Interested candidates who will be at M&M 2002 can contact me during the week to meet during the conference. I can be reached at 1-800-232-8691 x 5283 (best) or by leaving a message with the M&M message center or email at e2jrr-at-iname.com
South Bay Technology, Inc., a leading Materials Processing Equipment manufacturer, has several positions available including:
Field Sales Engineer
Applications Laboratory Technician
In-House Sales
All of these positions will be located in our Southern California headquarters which is located midway between Los Angeles and San Diego in the beachfront community of San Clemente, CA
For complete job descriptions, please contact:
Human Resources South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA
TEL: +1-949-492-2600 FAX: +1-949-492-1499
Email: jobs-at-southbaytech.com
We will also have these jobs posted at the Microscopy & Microanalysis Meeting in Quebec City. If you plan to be there, please visit us in Booth 1032.
Best regards-
David -- David Henriks TEL: +1-949-492-2600 South Bay Technology, Inc. FAX: +1-949-492-1499 1120 Via Callejon San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
Dear EM Community, I've had a heavy response to this posting and so I've decided to send 1 set of cassettes and boxes to 3 different educational institutions. 1 set to U of Penn, 1 set to Oklahoma State and 1 set to the Georgia Institute of Technology. I believe this will be a better way to share the resource.
Regards,
Joe Goodhouse Confocal / EM Core Laboratory Department of Molecular Biology Princeton University 609-258-5432
Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
Do any of you ingenious types out there know if it is possible (and if it is, please tell me how) to rotate the 840 OM about an axis radial to the column so that the eyepiece points directly upwards rather than towards the operator?
I am using a CCD video camera to look into the eyepiece, and such a rotation would make it much easier to mount the camera.
It works well, incidentally, much more convenient to use than leaning forward.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
I have interceeded and blocked the rapidly escalating Email flame/war on this Listserver by 2 individuals with the subject line
Hello All,
Forgive me if my question is elementary, but I am just beginning to so some simple brightfield photomicroscopy. Is there a simple formula for calculating the total magnification achieved when a photo is printed? For example, if you have 10X eyepieces and use a 40X objective when the photo is exposed, and then print it to a size of 3" X 4" on a journal page, can you easily calculate the final magnification?
Also, if such formulae do exist, were they developed with the assumption that one is using a 35mm camera? I am using a Nikon D1X (their top of the line digital). This camera has a chip, and that chip is different from the one used in a Nikon Coolpix 990. The projector lens in the tube of my compound microscope is 1.6X.
Thank you for any light anyone can shed on how to do these calculations.
on 8/2/02 12:25 AM, "JLCastner-at-aol.com"-at-sparc5.microscopy.com at "JLCastner-at-aol.com"-at-sparc5.microscopy.com wrote:
} } Forgive me if my question is elementary, but I am just beginning to so } some simple brightfield photomicroscopy. Is there a simple formula for } calculating the total magnification achieved when a photo is printed? For } example, if you have 10X eyepieces and use a 40X objective when the photo is } exposed, and then print it to a size of 3" X 4" on a journal page, can you } easily calculate the final magnification? } } Also, if such formulae do exist, were they developed with the assumption } that one is using a 35mm camera? I am using a Nikon D1X (their top of the } line digital). This camera has a chip, and that chip is different from the } one used in a Nikon Coolpix 990. The projector lens in the tube of my } compound microscope is 1.6X. } } Thank you for any light anyone can shed on how to do these calculations. } Dear Jim, First, I would calibrate the magnification of the image by taking a picture of a standard slide, then I'd use the image processing program to draw a bar on the image file corresponding to a known distance (e.g., 10 micrometers). When the image is printed in the journal--at any size the journal chooses--that bar will still represent the same distance. If you want the actual magnification, measure the length of the bar as it appears on the journal page and divide by the distance. Good luck. Yours, Bill Tivol
Dear netters I am curious if there is a good way of separating submicron thin films from silicon or silica substrate for TEM observation? I guess that hydrfluoric acid would work for most materials even though it has problem of toxicity.
Jim, Here is what I have experienced, but the best thing would be to test this using an object of know dimension.
1. The magnification from object to image on the photosensor = M(objective) x M(projector lens) 2. The magnification of the image from photosensor to paper = Dimension(paper)/Dimension(sensor) 3. The overall magnifaction is the product of these two = M(o)xM(p)xD(p)/D(s)
The same formula works for magnification on your computer/video screen.
Everett Ramer Cellomics, Inc. Pittsburgh, PA
-----Original Message----- } From: "JLCastner-at-aol.com"-at-sparc5.microscopy.com [mailto:"JLCastner-at-aol.com"-at-sparc5.microscopy.com] Sent: Friday, August 02, 2002 12:26 AM To: Microscopy-at-sparc5.microscopy.com
Hello All,
Forgive me if my question is elementary, but I am just beginning to so some simple brightfield photomicroscopy. Is there a simple formula for calculating the total magnification achieved when a photo is printed? For example, if you have 10X eyepieces and use a 40X objective when the photo is
exposed, and then print it to a size of 3" X 4" on a journal page, can you easily calculate the final magnification?
Also, if such formulae do exist, were they developed with the assumption
that one is using a 35mm camera? I am using a Nikon D1X (their top of the line digital). This camera has a chip, and that chip is different from the one used in a Nikon Coolpix 990. The projector lens in the tube of my compound microscope is 1.6X.
Thank you for any light anyone can shed on how to do these calculations.
Jim, Here is what I have experienced, but the best thing would be to test this using an object of know dimension.
1. The magnification from object to image on the photosensor = M(objective) x M(projector lens) 2. The magnification of the image from photosensor to paper = Dimension(paper)/Dimension(sensor) 3. The overall magnifaction is the product of these two = M(o)xM(p)xD(p)/D(s)
The same formula works for magnification on your computer/video screen.
Everett Ramer Cellomics, Inc. Pittsburgh, PA
-----Original Message----- } From: "JLCastner-at-aol.com"-at-sparc5.microscopy.com [mailto:"JLCastner-at-aol.com"-at-sparc5.microscopy.com] Sent: Friday, August 02, 2002 12:26 AM To: Microscopy-at-sparc5.microscopy.com
Hello All,
Forgive me if my question is elementary, but I am just beginning to so some simple brightfield photomicroscopy. Is there a simple formula for calculating the total magnification achieved when a photo is printed? For example, if you have 10X eyepieces and use a 40X objective when the photo is
exposed, and then print it to a size of 3" X 4" on a journal page, can you easily calculate the final magnification?
Also, if such formulae do exist, were they developed with the assumption
that one is using a 35mm camera? I am using a Nikon D1X (their top of the line digital). This camera has a chip, and that chip is different from the one used in a Nikon Coolpix 990. The projector lens in the tube of my compound microscope is 1.6X.
Thank you for any light anyone can shed on how to do these calculations.
Hi, We are looking to buy a CCD camera and software for mostly fluorescent microscopy. We will need a cooled system and a highly sensitive camera. The main microscope we will use it on is a Leica MZ FLIII. Any recommendations on what to buy? As always, are price, performance and relative simplicity of interest.
TGIF greetings to everyone, Does Leica provide repair service for the LKB knifemakers? Are there other service providers out there? thanks, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) **********************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
We bought one for 10'220 Euro directly off the company (they are an OEM manufacturer for others, like Leica, but their software support is maybe worth a look at). --
Wolf Schweitzer MD, LEGAL MEDICINE FMH (Switzerland) Institute of Legal Medicine Winterthurerstrasse 190 8057 Zuerich, Switzerland Tel. ++41 1 635 56 22
When Bill said "standard slide" I hope he was refering to a stage micrometer. These can be purchased from most EM suppliers. Once you take a picture at each objective mag, you can calculate the negative mag. Please note that the eyepiece plays no part in the negative magnification unless you have an eyepiece in the image path of the camera. Is you do, it will be included in the calculated mag from the negative.
Mannie Steglich Tech Dir. Pathology E M Lab U T M D Anderson Cancer Center
We use carbon support films for TEM preparations which we normally prepare in diffusion pumped Denton and Ladd evaporators. We have recently added a turbopumped evaporator from another supplier, but have not been happy with manufacturer support when we have had problems. We run 24/7 with multiple users and frequent air/vacuum cycles, sometimes several per hour.
We would appreciate hearing user experience on various models and suggestions as to our upcoming replacement purchases. Please reply offline to tmckee-at-scilabs.com or call at 800-476-5227. I will be happy to compile the responses.
Thanks in advance for your help.
Tom McKee tmckee-at-scilabs.com Compliance Officer
Scientific Laboratories, Inc. check us out at www.scilabs.com 13635 Genito Rd. Midlothian, VA 23112 804-763-1200, Fax 804-763-1800
I'm new to the field and still have to get our recently acquired EM400T working before I can actually try my hand at TEMicroscopy. After months of preparations, last Tuesday was the big day when I finally threw the giant main power switch on the wall for the first time and hoped to maniacally proclaim, "IT'S ALIVE!!" Well, I'm glad I didn't invite spectators for this one because I didn't get very far, but at least the EM400 showed a few signs of life.
Once I took care of a nasty hose leak and got the pneumatic pressure up high enough, the one symptom that stalled my progress occurs the same way each time, always about 20 seconds after powering up the EM400 (rotary pvp running). Suddenly, valve V1 begins rapidly opening and closing at a pretty regular pace (somewhat better than once per second and sometimes faster), and this is also observable at the V1 LED on the pump system indicator schematic panel on right side of the lower cabinet.
I've looked at the pullout pcb that's supposed to control V1, and I've tested the capacitors, but not the other components yet. (The ten micro farad one didn't test correctly until I took it out of the circuit, but the numbers looked good on the others while in circuit, so I didn't take them out.)
Has anyone encountered this problem before or have an idea where I might look next? I don't think it is the valve itself unless the LED indicator is designed to monitor the valve rather than the electronics that control the valve.
Thanks much for any tips!
Best, -Eric -- Eric Anderson SCSU Physics Adjunct 203-392-6455 anderson_e-at-southernct.edu
From root Fri Aug 2 15:32:40 2002 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id PAA23711 for dist-Microscopy; Fri, 2 Aug 2002 15:11:47 -0500 (CDT) Received: from njz_spm_filter (sparc5 [206.69.208.10]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id PAA23708 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Fri, 2 Aug 2002 15:11:16 -0500 (CDT) Received: from mail1.superlink.net (mail1.superlink.net [209.236.128.96]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id PAA23701 for {Microscopy-at-sparc5.microscopy.com} ; Fri, 2 Aug 2002 15:11:04 -0500 (CDT) Received: (qmail 759 invoked from network); 2 Aug 2002 16:06:30 -0400 Received: from maxtnt02-39.ewrnj.fast.net (HELO micro) (209.92.205.39) by superlink.net with SMTP; 2 Aug 2002 16:06:30 -0400 Message-ID: {00c101c23a61$3b9a4580$c9cd5cd1-at-fast.net}
Just keep your Denton 502, it will last forever!!!!!!!!1 Regards, Markus F. Meyenhofer Microscopy Labs ----- Original Message ----- } From: "Tom McKee" {tmckee-at-scilabs.com} To: "Microscopy List Service" {Microscopy-at-sparc5.microscopy.com} Sent: Friday, August 02, 2002 11:19 AM
The most direct way is to divide the measured length of the photo image of a stage micrometer by its actual size. For example, if the photo image is 40 mm wide and the actual distance at the object plane 0.08 mm, then the actual magnification is X500 (i.e., 40 / 0.08 = 500).
Note that the multiplication sign precedes the magnification number for the image (e.g., X500), but comes after the magnification number for the objective (e.g., 40X) by convention.
Gary Gill
-----Original Message----- } From: Everett Ramer [mailto:eramer-at-cellomics.com] Sent: Friday, August 02, 2002 8:11 AM To: 'Microscopy-at-MSA.Microscopy.Com'
Jim, Here is what I have experienced, but the best thing would be to test this using an object of know dimension.
1. The magnification from object to image on the photosensor = M(objective) x M(projector lens) 2. The magnification of the image from photosensor to paper = Dimension(paper)/Dimension(sensor) 3. The overall magnifaction is the product of these two = M(o)xM(p)xD(p)/D(s)
The same formula works for magnification on your computer/video screen.
Everett Ramer Cellomics, Inc. Pittsburgh, PA
-----Original Message----- } From: "JLCastner-at-aol.com"-at-sparc5.microscopy.com [mailto:"JLCastner-at-aol.com"-at-sparc5.microscopy.com] Sent: Friday, August 02, 2002 12:26 AM To: Microscopy-at-sparc5.microscopy.com
Hello All,
Forgive me if my question is elementary, but I am just beginning to so some simple brightfield photomicroscopy. Is there a simple formula for calculating the total magnification achieved when a photo is printed? For example, if you have 10X eyepieces and use a 40X objective when the photo is
exposed, and then print it to a size of 3" X 4" on a journal page, can you easily calculate the final magnification?
Also, if such formulae do exist, were they developed with the assumption
that one is using a 35mm camera? I am using a Nikon D1X (their top of the line digital). This camera has a chip, and that chip is different from the one used in a Nikon Coolpix 990. The projector lens in the tube of my compound microscope is 1.6X.
Thank you for any light anyone can shed on how to do these calculations.
Sincerely,
Jim Castner
From root Fri Aug 2 17:02:52 2002 Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: (from daemon-at-localhost) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id QAA24628 for dist-Microscopy; Fri, 2 Aug 2002 16:38:23 -0500 (CDT) Received: from njz_spm_filter (sparc5 [206.69.208.10]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id QAA24026 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Fri, 2 Aug 2002 16:32:02 -0500 (CDT) Received: from mtiwmhc23.worldnet.att.net (mtiwmhc23.worldnet.att.net [204.127.131.48]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with ESMTP id QAA24015 for {Microscopy-at-sparc5.microscopy.com} ; Fri, 2 Aug 2002 16:31:51 -0500 (CDT) Received: from vitaly ([12.92.213.115]) by mtiwmhc23.worldnet.att.net (InterMail vM.4.01.03.27 201-229-121-127-20010626) with SMTP id {20020802212717.BKKN7441.mtiwmhc23.worldnet.att.net-at-vitaly} ; Fri, 2 Aug 2002 21:27:17 +0000 Message-ID: {009001c23a6b$30c195a0$73d55c0c-at-vitaly}
Hi Carl,
We used http://www.fli-cam.com/ , http://www.sbig.com/ for slow scan only, check software at http://www.cyanogen.com/index2.html . For slow scan/live video try http://www.dvcco.com/ . Results were pretty good with all these cameras. Many others do exist which we didn't try. Contact me off list for specific information.
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax (678)467-0012 mobile
This message is made of 100% recycled electrons. ----- Original Message ----- } From: Carl Dahlberg {carl.dahlberg-at-kmf.gu.se} To: {Microscopy-at-sparc5.microscopy.com} Sent: Friday, August 02, 2002 10:13 AM
Eric,
Is the V1 physically opens? You can see that with the naked eye. If yes, then proceed further.
First, you have to put a V1 control card (E-U12A) on the extender card, and check the V1 control signals- there are several, each one easily traceable to the point of origin- Philips manual has good schematics with the detailed explanation. You will see at least one signal going through the same cycle as the valve does. Use fast response meter with hold function, oscilloscope is even better, as the signal level may change in a matter of milliseconds back and forth. Very generally, switches and pneumatic components are more likely to fail as compared with electronic components. A number of time delays are incorporated into vacuum logic sequences. Problem may be in one of the delay circuits. You need to trace the faulty signal first.
If I have to guess what's wrong, here it is: 1) Pneumatic safety switch contacts are ringing (replace switch or use larger capacitor at it's input filter on the E-U13A card, or increase air pressure within the reasonable limits) 2) Pneumatic solenoid which controls V1 is defective 3) V1 end switch is not engaged, or is defective
Just a guess, could be something other.
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax (678)467-0012 mobile
This message is made of 100% recycled electrons. ----- Original Message ----- } From: Eric Anderson {andersone1-at-southernct.edu} To: 'microscopy-at-msa.microscopy.com' {microscopy-at-sparc5.microscopy.com} Sent: Friday, August 02, 2002 1:49 PM
Eric, I'm not familiar with the EM400T, but I've seen this kind of behavior if there is a pressure switch for detecting an air pressure failure. If the pressure is marginal, opening the valve drops the pressure enough to trip the air pressure failure circuit causing the valve to close. With no flow on the airline, the pressure recovers and resets the circuit and allows the valve to open again, dropping the air pressure. Try increasing the air pressure another 10 psi and see if a) the problem goes away or b) the frequency of valve operation changes. If nothing changes, then you probably have an electrical or electronic problem. If the frequency changes, look for restrictions in your air lines.
Ken Converse owner Quality Images third party SEM service Delta, PA
Eric Anderson wrote:
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There are two pneumatic valves located in the pump space, just to the right of the roughing pump and each of those valves has a small microswitch which is used to sense whether the valve is open or closed. The problem can frequently be eliminated by simply adjusting the position of the switch. This is accomplished by adjusting a small screw which is part of the switch itself.
Good luck and congratulations on resurrecting an EM-400. It is a fine instrument.
Alex Greene SCIENTIFIC INSTRUMENTATION SERVICES, INC. PMB-499, 1807 West Slaughter Lane, Number 200 Austin, Texas 78748-6200 Phone 512/282-5507 FAX 512/280-0702
Sustaining Member - MICROSCOPY SOCIETY OF AMERICA ----- Original Message ----- } From: "Eric Anderson" {andersone1-at-southernct.edu} To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-sparc5.microscopy.com} Sent: Friday, August 02, 2002 12:49 PM
Jondo;
When you say "submicron", how thin do you mean? I uM = 10,000 angstroms. Is it 100 angstroms or 5,000?
If the film is thick enough, you may be able to fracture a section off mechanically. I assume you want the film as a sheet of material rather than in x-section as one might get with a FIB for TEM samples.
You may attempt this chemically if your substrate will dissolve or etch in something that will not etch or change the characteristics of your film. You may be able to simply dissolve off the substrate material.
A SERIOUS CAUTION ABOUT USING HF.
Hydrofluoric acid is not just toxic but can cause very serious injury to human tissue and should only be used by someone that is trained and experienced in it's handling, disposal and all safety hazards. Please find out first what precautions to take such as skin and eye protection. HF, unlike acids such as sulfuric or nitric, will not initially burn and will simply feel like a skin irritation. You have only minutes to treat the affected site. In ADVANCE, you should set up with your safety officer a hospital emergency room that is prepared to treat you if there is an accident. They need to know the treatment protocol or you may lose a limb while the medical staff finds out what to do. I say all this from experience and a serious regard for safety. We use HF routinely for etching SiN, SiO2 etc.
Peter Tomic Anadigics, Inc.
-----Original Message----- } From: Jondo Yun À±Ážµµ [mailto:jdyun-at-kyungnam.ac.kr] Sent: Friday, August 02, 2002 4:04 AM To: MicroscopyListserver
Dear netters I am curious if there is a good way of separating submicron thin films from silicon or silica substrate for TEM observation? I guess that hydrfluoric acid would work for most materials even though it has problem of toxicity.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (alopes966-at-aol.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday, August 3, 2002 at 18:22:16 ---------------------------------------------------------------------------
Email: alopes966-at-aol.com Name: Alexandre Lopes
Organization: INESP - DivinÛpolis - Brasil
Education: Graduate College
Location: DivinÛpolis - Minas Gerais - Brasil
Question: Oi, one question. How photography in microscopes? I'm professor of photography in Brasil and i never make this. You have sugestion for a program of class for biology or wildlife for exemple. If you have same kind of catalog, brochure or text about your organization,or simple "old" photomagazines, please send for:
Alexandre Lopes Rua JosÈ de Alencar, 540 Nova Suissa Belo Horizonte Minas Gerais Brasil CEP 30480500
My interess is history, new photo-artists and contemporanean art photo, ok. Thank You Very Much
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ao_reu-at-ccmr.cornell.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday, August 3, 2002 at 06:48:45 ---------------------------------------------------------------------------
Question: Hello, I have been working on the preparation of equiatomic NiTi for EBSD analysis this summer. As a final polishing step, I have been trying to electropolish my samples. It seems to do a good job removing the metal from the sufrace, but sometimes it leaves a build-up of something on the surface of the samples. I was just wondering what this build up might be and how I might be able to avaoid this happening while I'm electropolishing or if there is some way (non-mechanical) of removing the layer after electropolishing. Any suggestions would be greatly appreciated as I am quickly running out of time to work on this.
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A couple of possible causes:
1. Your compressed air feed is just too low. As the valve moves, the pressure in the supply drops slightly, causing the microscope to close the valve. Try turning up the pressure ~0.5 bar.
2. There's a problem with the solenoid-driven valve that is switching the pneumatic lines. These valves are in a bank under the flat panel just to the right of the column. The electronics and the solenoid are probably OK but the pneumatic valve itself may be failing. To start with, simply switch the valve with another (the acctuating solenoids are easily slid off), to confirm the problem. On an old EM400T, you may need to replace all of these valves to get the vacuum system working reliably.
Send a message including the following: Sunscribe {Full name} to: LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU Here is there webb interface address as well. http://listserv.acsu.buffalo.edu/cgi-bin/wa
Hope this helps
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Fred Monson
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center c/o Geology/Astronomy West Chester University South Church Street and Rosedale Ave West Chester, Pennsylvania, USA, 19383 Phone: 610-738-0437 FAX: 610-738-0437 fmonson-at-wcupa.edu CASI URL: http://darwin.wcupa.edu/casi/ WCUPA URL: http://www.wcupa.edu/ Visitors URL: http://www.wcupa.edu/_visitors/
} ---------- } From: Mike Delannoy } Sent: Friday, August 2, 2002 9:43 AM } To: microscopy } Subject: re:confocal listserver } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear listserver, } Could someone post the address of the confocal listserver again? } } thanks } Mike D. } } }
I just reread your original post. Let me clarify my previous response. When you use a stage micrometer it doesn't matter what kind of camera you use. In your case, use your Coolpix and photograph the scale on the stage micrometer. Make a print of it the same size you make of anything else photographed using the same objective lens. Do not crop anything off of either print, print the entire frame. Pick a distance of say 2mm on the ruler on the print. You can then use a ruler and using the formula - #mm on the ruler you are measuring with (lets say 50) divided by the # of mm on the print (we said 2). This would give you a print magnification of x25. If you are using film, you can calculate the negative magnification for each objective by measuring the ruler on the negative. Then you would need to calculate the final magnification of the print using the negative mag times the enlargement magnification.
It is once again that time of year that many of us meet at the Microscopy and Microanalysis meeting. As in previous years we will be streaming video from the Computer workshop area. Feel free to login and virtually join the meeting even if it is just looking over someones shoulder. We will move the camera around at different times during the week.
I have a student with HIV infected tissue embedded in resin. Does anyone know whether the HIV survives through all the steps OsO4, UAc, EtOH, acetone, Epon-Araldite? A library and Web search yielded nothing useful.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (RUCHIKA781-at-INDIATIMES.COM) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, August 5, 2002 at 23:21:54 ---------------------------------------------------------------------------
Question: I am interested to know about a microscope that can provide 500x magnification covering at least 20mm field of view. What should be its specification?
Hi Diana, Treatment for EPON is very harsh for living tissues. I don't think HIV does survive this kind of treatment. It does'nt even survive freezing (source: an MD ), so imagine with fixative, osmium, ETOH, PO, EPON, heat... If you are too worried check with a virologist to be 100% sure. Emmanuelle
Ruchika no microscope available can cover a field as large as 20mm in a single image at 500x. The image would be 1 metre wide! A field width of 0.25 x 0.20 mm would be nearer the mark - if the final image magnification is 500 times when enlarged to fit onto 5"x4" film.
Many modern microscopes use digital cameras, and there are several software packages that can stitch multiple images together to cover larger areas than the microscope's limited field width. However, at 500x you would need to stitch several thousand images together (at 0.25x0.2mm, 80x100=8000 images), and the final file size (1 image = minimum 1Mb: 8000 images =8Gb)would defeat all but the most powerful computers. Certainly not a job for the old PC. Well, not mine anyway!
I would put the question back to you and ask why do you need to cover all of a 20mm specimen at that magnification? Chris
Date sent: Tue, 6 Aug 2002 08:49:31 -0500 To: microscopy-at-sparc5.microscopy.com } From: RUCHIKA781-at-INDIATIMES.COM (by way of MicroscopyListserver)
Hello list,
I'm looking for advices on how to move a Jeol JEM 100CX TEM.
I got a Jeol JEM 100CX this spring if I only moved it myself. After a lot of problems I finally have found a suitable place to put it in, so the next step is to move it. I will probably start picking it apart in 1-2 weeks time. The only problem is that I have never done it before and I don't have the budget to hire a technician to do it for me.
I'm doing this as a private hobby project to learn about EM, vacuum technology and just for fun. I really hate seeing good old instruments being scrapped. :-)
I'm not all by my self. I have a number of friends that have offered to help with all from repairing the electronics (the OL-current control is broken) to actually help lifting and driving the truck.
The plan is to use common sense, the mechanical drawings of the column and a digital camera to document every step when we take it apart. Then we might have a fighting chance to put it back again. I have talked with the Swedish representant of Jeol but they claimed they didn't have any documentation on how to move or put it together. They sounded so negative so I don't think I have much help to get there. Maybe they discovered that I wasn't a customer with a fat wallet. :-)
As I have learnt a lot from this list by lurking and reading the archives, (Thanks Nestor!) I now try to get some advice to how I should move the TEM. And more importantly, what I should avoid to do! I'm happy for any advice of horror story you want to share. If you have some documents to share, I could pay for copying cost and postage, just let me know.
Whatever the result is I haven't payed much for it, so if I fail to get it running again I will probably have learnt a lot and if I get it running I also get a TEM. I just can't loose! :-)
The diary and details of my EM adventure is documented on http://www.home.neab.net/gandalf/EM-lab/index.htm Anyone wanting to see the inside if the OL-current control? Just follow the link.
If you read this far you are either laughing or just shaking your head. Thanks anyhow.
Thanks to all for your advice and guidance. Once the pneumatic pressure was increased from 65 to about 80 psi (5.5 bar) as suggested, V1 stopped opening & closing. I also found and repaired a wire that had broken away from the tapered-rod-activated-microswitch on the pneumatic valve down below that's associated with V1. But now, the rotary pvp (a new Alcotel2012) just shuts off after only a couple of minutes running (Main display panel then has no PV's lit.), and after another couple of minutes, the power supply shuts down. (Just once, the pumping system prematurely switched from PV1 to PV2 for a minute or so before shutting off.)
This impressive piece of equipment was partly dismantled and in storage for a few years, plus has experienced a good many miles of bumpy roads and dirt on the way to its current home. If the budget allows, I think we'll soon have to hire an experienced EM400 technician to more efficiently carry out what I expect to be a fair amount of troubleshooting/alignment here. I'm just trying to solve a few of the simpler problems beforehand, before I get busy with the fall semester.
Thanks again for all of your help!
Kind regards, -Eric
Eric Anderson SCSU Physics Adjunct 203-392-6455 anderson_e-at-southernct.edu
------------------------------------------ Larry Stoter wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hello folks! } } } } I'm new to the field and still have to get our recently acquired EM400T } } working before I can actually try my hand at TEMicroscopy. After months of } } preparations, last Tuesday was the big day when I finally threw the giant } } main power switch on the wall for the first time and hoped to maniacally } } proclaim, "IT'S ALIVE!!" Well, I'm glad I didn't invite } } spectators for this one because I didn't get very far, but at least the } } EM400 showed a few signs of life. } } } } Once I took care of a nasty hose leak and got the pneumatic pressure up } } high } } enough, the one symptom that stalled my progress occurs the same way each } } time, } } always about 20 seconds after powering up the EM400 (rotary pvp running). } } Suddenly, valve V1 begins rapidly opening and closing at a pretty regular } } pace (somewhat better than once per second and sometimes faster), and this } } is also } } observable at the V1 LED on the pump system indicator schematic panel on } } right side of the lower cabinet. } } } } I've looked at the pullout pcb that's supposed to control V1, and I've } } tested the capacitors, but not the other components yet. (The ten micro } } farad one didn't test correctly until I took it out of the circuit, but the } } numbers looked good on the others while in circuit, so I didn't take them } } out.) } } } } Has anyone encountered this problem before or have an idea where I might } } look next? } } I don't think it is the valve itself unless the LED indicator is designed } } to monitor the valve rather than the electronics that control the valve. } } } } Thanks much for any tips! } } } } Best, } } -Eric } } -- } } Eric Anderson } } SCSU Physics Adjunct } } 203-392-6455 } } anderson_e-at-southernct.edu } } A couple of possible causes: } } 1. Your compressed air feed is just too low. As the valve moves, the } pressure in the supply drops slightly, causing the microscope to } close the valve. Try turning up the pressure ~0.5 bar. } } 2. There's a problem with the solenoid-driven valve that is switching } the pneumatic lines. These valves are in a bank under the flat panel } just to the right of the column. The electronics and the solenoid are } probably OK but the pneumatic valve itself may be failing. To start } with, simply switch the valve with another (the acctuating solenoids } are easily slid off), to confirm the problem. On an old EM400T, you } may need to replace all of these valves to get the vacuum system } working reliably. } } Regards, } -- } Larry Stoter--
I think you are on the right way: use your common sense and everything should be OK. What I would do in such case:
-turn ON microscope and let air enter the column/gun/camera using regular cycle. -turn OFF the microscope (key OFF), unplug from water/electricity/air sources. -remove the cover to have access to the column; - you have to disassemble column; -label all electrical connections - both sides which connected to the column and cabinets. -label all vacuum/air/water tubings - both sides - same as before; -label all parts you are going to disassemble - I would suggest you may make a few marks for clear indication of the correct orientation. I also suggest you will attach screws to the place where they were in some small plastic bag - it's easier than mark all screws! Just in case, mark plastic bag as well! - disconnect all electrical cables from the column and from the cabinets, checking that everything is marked at the both ends! - similarly disconnect water/air etc.
-Have a lot of heavy-duty alumina foil ready!
-Column, upper part at the level of the objective lens (so the lens would be in the lover part): detach the vacuum/air etc lines and diffusion pump, use some wood planks (strong enough) to secure the upper part of the column: put 4-5 planks around the column and secure them with some strong metal belts (from Home Depot if you have some?). This construction should be strong enough to hold the whole weight of the column. You'll use it to move the column (upper part). Unscrew the bolts holding this part of the column (between the segments of the column). Then: 5 strong men will gently move up this part of the column and carefully lay it on the floor with alumina foil. Use alumina foil to protect the openings in the column! Check, you don't lost O-ring. Be sure that you do not damage aperture assembly and valves/connections on the column when moved it. Cover all openings (even electrical connectors) with alumina foil!!!!!!
- use another sheet of the foil to protect opening on the lower part of the column/connections etc
- I don't remember exactly, but it seems that 100CX has a central console with column and two detachable cabinets. You need to try to detach left/right cabinets (checking for all connections/tubing) from the central console.
Finally you have to have the following separate parts:
-air compressor; -mechanicam pump assembly; -power supply; -central console with 1/2 column; -upper part of the column; -left and right cabinets.
Now, make measurements to insure you may move out all parts. If it's OK - you are very lucky! This scenario permits to move parts within the floor. You probably could not use the elevator, it's heavy. You have to check. If this parts are too big/heavy to move out, your business is probably hopeless. If you'll disassemble the column on the smaller pieces, you will need professional help with column's mechanical alignment.
When assemble: clean up all vacuum O-rings (and their sits) with hexane or 95% Ethanol, apply tiny amount of the Apiezone-N on the O-rings (very little) and assemble in reverse order. When installed upper part of the column - be sure you orient column correctly and move it very slow. otherwise you may damage O-ring. Be sure everything is clean and you do not spoil some dust into the lover part of the column when install upper part... I am sorry, I am trying to do my the best... I hope it helps. Good luck. Sergey.
P.S. Some remarks: you have to keep original orientation of the console and cabinets: do not let leak oil from DP or HV tank. Move vertical and horizontal only, do not tilt. Be careful with water lines - do not spoil the water on electronics etc... If you have questions - ask off line if you wish. I hope you'll enjoy playing with 100CX in the Lego. I like such games. Russians says: "Who is not risking, that person will not drink champagne at the end". I wish, you will.
At 03:36 PM 8/6/02, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Good morning all at the MSA listserver, I am looking for reference material that explains proper techniques for tissue collection and fixation. Currently I am receiveing tissue that is quite autolytic and I can't seem to find the words to get the idea that tissue ultrastructure, such as liver and kidney, is extremely vulnerable to hypoxia (such as when an animal is exsangunated), and delayed fixation. One concern is that some feel that tissues can be collected at room temperature in room temperature fixative, especially liver and kidney. In my training, these particular tissues needed to be collected cold! and placed in cold fixative to stop metabolism and autolysis. I need referenced help???
Thanks in advance, Connie
P.S. Hope everyone is having fun at MSA this year!!!!
HIV, like cell components, if prepared properly survives just fine in routine preparations for cell embedment. Many beautiful micrographs have been published of it. Run a search in PubMed or Medline for journal articles. It is also pictured in several virology atlases.
Sara Miller
Quoting Diana van Driel {dianavd-at-eye.usyd.edu.au} :
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have a student with HIV infected tissue embedded in resin. Does } anyone know whether the HIV survives through all the steps OsO4, UAc, } } EtOH, acetone, Epon-Araldite? A library and Web search yielded } nothing useful. } } Cheers, } } Diana } } }
In response to your request for information about moving a JEOL 100CX TEM there should not be a great deal of difficulty in moving the system or in getting it up and running. I have moved dozens of SEM and TEM (and other) instruments and with a little common sense and care the move should actually be quite easy. A couple months ago I moved a Philips EM100 (about the same vintage as the JEOL 100CX) and the tear down from running to leaving on the truck took about 5 hours with two people along with Johnson bars, some 4x4 and 2x4 wood sections and a pallet truck. I hope your move is an adventure rather than a nightmare.
The first order of business is to make sure that the TEM is shut down properly (as if for normal servicing) so that the column has been leaked up to air. If the center console unit will fit through the doors that you need to maneuver through then you might consider removing the gun assembly at the top of the column then putting on a blank-off and evacuating the remainder of the column. You could then move the system under vacuum and make the restart a much simpler operation. If you are going to need to move the system up and down stairways rather than using an elevator, then the tear-down will need to be much more involved and the re-assembly will be a lot more difficult.
Anyway, once the column has been leaked, you should first remove any and all decorative or cover panels from the column assembly and the electrical consoles to make the disassembly process easier. Mark each as to its location so that it will be simpler when reassembling the system. Next remove the gun assembly with its high voltage cable. Some of these are oil filled to prevent electrical arcing so you should take care to maintain the gun assembly in an upright position so as to avoid oil spillage. Then remove the other end of the cable from the high voltage tank (which I think is under the left hand wing console on a JEOL 100CX but am not certain). Take care not to short the high voltage contacts through this process as there may be residual high voltage in the tank. Put a plug (usually a screw cap included on the JEOL tanks) in the high voltage tube on the HT tank. If there is not a plug attached to the tank then use a rubber cork with some duct tape to hold it in place.
You will next need to remove the table top to expose the three separate console units for disassembly and drain any water from cooling lines to avoid spillage into the column or into the electronics assemblies.
At this point you will need to decide how far to tear down the column to make the move with the equipment you have available. We usually start by removing the left side wing console, removing all of the wiring connections (the harnesses should be kept intact) either from the wing console or from the column or right wing console (whichever is easier), sliding out the HT tank and unscrewing the console from the main unit. This should produce a relatively light weight console with some enclosed electronics, the HT tank and any panels that needed to be removed in order to get to the bolts holding things together. Next, remove all of the electrical connections to the electron beam column and to the main console and bundle these together with the right hand wing column (again, I may have the location of the HT tank reversed as to which console it is enclosed in (or it may be separate altogether but I don't remember that as being the case for the 100CX). Anyway, then remove the right hand wing console to produce another relatively light weight section that can be moved. This leaves the pumping system and the main console that includes the column. If you have the space for moving this section as a complete unit, that is the easiest way to make sure the system will be up and running in a relatively short time. If you need to make the whole thing lighter, start by removing the pumping system as a unit (if possible) or remove each pumping component separately and make sure you mark the locations of mating tubing, hoses, electrical connections, etc. With the pumping system removed you should have left only the main console, the column and any attached electronics (with the cables either bundled here or removed and bundled with one or the other of the wing consoles, whichever was easier). If the weight, height, etc. is still too great to move this section as is, you will need to remove each column section one at a time starting at the top, marking the positions of any electrical or water connections and keeping track of the o-ring that is associated with each break point (along with bagging and marking the screw or bolt set that you removed so they don't get confused with any others). You can remove everything down to the tabletop level if you need to but this, of course, makes the re-assembly a much more time-consuming process. This leaves you with the system stripped down about as far as possible unless you want to remove separately each of the electronics units.
All of the smaller components can usually be moved by one or two people with four wheeled dollies or simply by carrying. The HT tank may be on its own wheels. If not it can be elevated onto a four wheel dolly either by lifting (3-4 people is best) or using a Johnson bar and wood spacers to lift it high enough to slide under a wheeled dolly. The same is true of the main console. If you have a small fork lift available the whole assembly can simply be lifted by sliding the forks under the console being sure that the weight is distributed well to prevent tipping. You may need to use cross pieces of wood to make sure of this. If you need to lift the column console onto a pallet jack, start by lifting either front, back or sides with a Johnson bar and sliding in a 2x4. Repeat on the opposite side, and then repeat the process replacing the 2x4 with a 4x4 (or adding another 2x4) as many times as needed to slide under the wheeled pallet jack or other device you have available so that the section can be moved. If you are going to move up and down stairways you will need to strip virtually everything from the main console so that one or two people can attach the console to a furniture dolly (or more people can lift the assembly).
In all this, make sure that when you remove or lift something you are not in danger of breaking water or electrical connections or any other components that might be bolted onto or protruding from the item you are moving. Take your time and this should not be a problem. In almost all cases, when we have broken something on a system it was because we were rushing to get done instead of taking our time.
Sorry to hear that you didn't get much help from the Swedish JEOL group. Here in the USA, JEOL service is one of the finest in the world. They have always been very helpful to me personally and to almost everyone with whom I have discussed them. As far as a manual, there are some in-house JEOL documents but they never did issue detailed user manuals for installation as some of the other EM suppliers have done. They always felt that the installation was a part of the purchase and sent their engineers to do the work.
I wish you luck in your TEM moving endeavor. As for your electronics problem, I unfortunately just scrapped out a good deal of JEOL electronics from older systems and no longer have any available, just some of the parts and pieces that we saved. When you collect the system, make sure you also get all of the manuals that are available for the system in its present location and, if at all possible, get the JEOL service record log (they always write a detailed description of their repairs). With the log you will likely be able to find and diagnose electronics problems and/or vacuum problems much more quickly since you can look through the records and see if the same or similar problem was worked on in the past and how it was fixed.
Again, good luck!!
Drew Hirt President/Senior Scientist Materials Research Laboratories, Inc. Struthers, OH USA drew-at-hirt.com mrllab-at-raex.com www.mrllab.com
This is certainly an interesting concern for many of us, I thought it might be worthwhile to mention it on the Network.
I spent more than 3 years doing EM studies of several types of HIV (HIV-1, HIV-2) and SIV at Harvard AIDS Institute, and we have no evidence that these lentiviruses can survive the harsh condition used for EM processing. Based on what we know, HIV is in fact very fragile and does not survive 70% ethanol and formalin fixation. It is unlikely that they will survive glutaraldehyde and osmium.
I personally fixed, processed, embedded, and sectioned over a thousand HIV and SIV-infected cell samples and some autopsies, but never had any problems with HIV infection. The fact that my colleagues and I are still alive, healthy, and HIV-negative 10 years after that period is another good evidence that it is safe to do EM on HIV-infected samples.
Of course one has to be extremely careful about the potential infection when dealing with these AIDS related samples. All specimens must be properly fixed inside the hood at a P3 laboratory before being sent out for EM processing. Even good practice of common sense precautions will help. However, I do not remember anyone has been able to reinfect cells with HIV isolated from glut-fixed and epoxy embedded samples.
QC Yu
Qian-Chun Yu, MB, Ph.D. Director Cell Imaging Core Abramson Cancer Research Institute University of Pennsylvania 421 Curie Boulevard, Room 532 Philadelphia, PA 19104
Someone needs to define here what is meant by "survives". Are we talking about preservation of ultrastructure or capacity for infection of microscopists?
Marie
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology University of Connecticut Unit 2242 Storrs, CT 06269-2242 Phone: 860-486-3588 Fax: 860-486-6369
} From: Materials Research Laboratories, Inc. {mrllab-at-raex.com} } Date: Wed, 07 Aug 2002 11:02:26 -0500 } To: {microscopy-at-sparc5.microscopy.com} } Subject: RE: Moving a Jeol JEM 100CX TEM } } Göran Axelsson {axelsson-at-acc.umu.se} } } In response to your request for information about moving a JEOL 100CX TEM } there should not be a great deal of difficulty in moving the system or in } getting it up and running. I have moved dozens of SEM and TEM (and other) } instruments and with a little common sense and care the move should actually } be quite easy. A couple months ago I moved a Philips EM100 (about the same } vintage as the JEOL 100CX) and the tear down from running to leaving on the } truck took about 5 hours with two people along with Johnson bars, some 4x4 and } 2x4 wood sections and a pallet truck. I hope your move is an adventure rather } than a nightmare. } } The first order of business is to make sure that the TEM is shut down properly } (as if for normal servicing) so that the column has been leaked up to air. If } the center console unit will fit through the doors that you need to maneuver } through then you might consider removing the gun assembly at the top of the } column then putting on a blank-off and evacuating the remainder of the column. } You could then move the system under vacuum and make the restart a much } simpler operation. If you are going to need to move the system up and down } stairways rather than using an elevator, then the tear-down will need to be } much more involved and the re-assembly will be a lot more difficult. } } Anyway, once the column has been leaked, you should first remove any and all } decorative or cover panels from the column assembly and the electrical } consoles to make the disassembly process easier. Mark each as to its location } so that it will be simpler when reassembling the system. Next remove the gun } assembly with its high voltage cable. Some of these are oil filled to prevent } electrical arcing so you should take care to maintain the gun assembly in an } upright position so as to avoid oil spillage. Then remove the other end of } the cable from the high voltage tank (which I think is under the left hand } wing console on a JEOL 100CX but am not certain). Take care not to short the } high voltage contacts through this process as there may be residual high } voltage in the tank. Put a plug (usually a screw cap included on the JEOL } tanks) in the high voltage tube on the HT tank. If there is not a plug } attached to the tank then use a rubber cork with some duct tape to hold it in } place. } } You will next need to remove the table top to expose the three separate } console units for disassembly and drain any water from cooling lines to avoid } spillage into the column or into the electronics assemblies. } } At this point you will need to decide how far to tear down the column to make } the move with the equipment you have available. We usually start by removing } the left side wing console, removing all of the wiring connections (the } harnesses should be kept intact) either from the wing console or from the } column or right wing console (whichever is easier), sliding out the HT tank } and unscrewing the console from the main unit. This should produce a } relatively light weight console with some enclosed electronics, the HT tank } and any panels that needed to be removed in order to get to the bolts holding } things together. Next, remove all of the electrical connections to the } electron beam column and to the main console and bundle these together with } the right hand wing column (again, I may have the location of the HT tank } reversed as to which console it is enclosed in (or it may be separate } altogether but I don't remember that as being the case for the 100CX). } Anyway, then remove the right hand wing console to produce another relatively } light weight section that can be moved. This leaves the pumping system and } the main console that includes the column. If you have the space for moving } this section as a complete unit, that is the easiest way to make sure the } system will be up and running in a relatively short time. If you need to make } the whole thing lighter, start by removing the pumping system as a unit (if } possible) or remove each pumping component separately and make sure you mark } the locations of mating tubing, hoses, electrical connections, etc. With the } pumping system removed you should have left only the main console, the column } and any attached electronics (with the cables either bundled here or removed } and bundled with one or the other of the wing consoles, whichever was easier). } If the weight, height, etc. is still too great to move this section as is, you } will need to remove each column section one at a time starting at the top, } marking the positions of any electrical or water connections and keeping track } of the o-ring that is associated with each break point (along with bagging and } marking the screw or bolt set that you removed so they don't get confused with } any others). You can remove everything down to the tabletop level if you need } to but this, of course, makes the re-assembly a much more time-consuming } process. This leaves you with the system stripped down about as far as } possible unless you want to remove separately each of the electronics units. } } All of the smaller components can usually be moved by one or two people with } four wheeled dollies or simply by carrying. The HT tank may be on its own } wheels. If not it can be elevated onto a four wheel dolly either by lifting } (3-4 people is best) or using a Johnson bar and wood spacers to lift it high } enough to slide under a wheeled dolly. The same is true of the main console. } If you have a small fork lift available the whole assembly can simply be } lifted by sliding the forks under the console being sure that the weight is } distributed well to prevent tipping. You may need to use cross pieces of wood } to make sure of this. If you need to lift the column console onto a pallet } jack, start by lifting either front, back or sides with a Johnson bar and } sliding in a 2x4. Repeat on the opposite side, and then repeat the process } replacing the 2x4 with a 4x4 (or adding another 2x4) as many times as needed } to slide under the wheeled pallet jack or other device you have available so } that the section can be moved. If you are going to move up and down stairways } you will need to strip virtually everything from the main console so that one } or two people can attach the console to a furniture dolly (or more people can } lift the assembly). } } In all this, make sure that when you remove or lift something you are not in } danger of breaking water or electrical connections or any other components } that might be bolted onto or protruding from the item you are moving. Take } your time and this should not be a problem. In almost all cases, when we have } broken something on a system it was because we were rushing to get done } instead of taking our time. } } Sorry to hear that you didn't get much help from the Swedish JEOL group. Here } in the USA, JEOL service is one of the finest in the world. They have always } been very helpful to me personally and to almost everyone with whom I have } discussed them. As far as a manual, there are some in-house JEOL documents } but they never did issue detailed user manuals for installation as some of the } other EM suppliers have done. They always felt that the installation was a } part of the purchase and sent their engineers to do the work. } } I wish you luck in your TEM moving endeavor. As for your electronics problem, } I unfortunately just scrapped out a good deal of JEOL electronics from older } systems and no longer have any available, just some of the parts and pieces } that we saved. When you collect the system, make sure you also get all of the } manuals that are available for the system in its present location and, if at } all possible, get the JEOL service record log (they always write a detailed } description of their repairs). With the log you will likely be able to find } and diagnose electronics problems and/or vacuum problems much more quickly } since you can look through the records and see if the same or similar problem } was worked on in the past and how it was fixed. } } Again, good luck!! } } Drew Hirt } President/Senior Scientist } Materials Research Laboratories, Inc. } Struthers, OH USA } drew-at-hirt.com } mrllab-at-raex.com } www.mrllab.com
Excellent suggestion! I took the second "definition" for my response as I feel "survival" is more related to "life" or viability. For ultrastructure, we often use "preservation", though sometimes we do use "survive". A precise definition seems necessary here as we already see two types of answers. Regards,
QC Yu
Qian-Chun Yu, MB, Ph.D. Director Cell Imaging Core Abramson Cancer Research Institute University of Pennsylvania 421 Curie Boulevard, Room 532 Philadelphia, PA 19104
ok first off there is no one proceedure for tissue fixation. when i was doing clinical work we would get kidney tissue sent down on saline soaked gauzr, perfectly find with as much as an hour delay. i have always fixed at room temperature with no real ill effects. there are perhaps 100s of Em books out on the market. if you must articulate then just say what you wrote here. john
I have a customer that is interested in having the thermal decomposition of sodium bicarbonate "videotaped", while undergoing SEM at probably 500X, maybe 1000X. He would be interested in seeing the honeycomb structure appear from the block-like particles that are seen at RT. (Sodium bicarbonate decomposes at around 100 deg. C.)
I was thinking a heated stage with either low voltage or low pressure SEM. Is anyone interested in quoting this project? Sounds interesting.
Good afternoon, I'd like to hear from anyone that has experience in the microscopy of waxes. Specifically, I am interested in the means: LM, TEM, other, that have been successfully used to detect the presence of waxes whether present as a thin film or sub-micron domains within a host polymer matrix.
Thanks, Paul
Paul J. Gerroir Microscopy Materials Characterization Xerox Research Centre of Canada 2660 Speakman Drive Mississauga, Ontario L5K 2L1
{TT} Hi everybody out there! {BR} {BR} My department is planning to possibly get rid of the {BR} darkroom set up for processing negatives from the TEM {BR} and SEM. {BR} Does anyone have any suggestions on digital set ups {BR} that we could go with that would give us the same {BR} versitility? Also average costs? {BR} {BR} {BR} Thanks, {BR} {BR} Khara L. Scott {BR} Electron Microscopy Technologists II {BR} Biological Sciences Chicago State University {BR} Chicago, Il {BR} {BR} {/TT}
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It's almost a compromise: to use low or room temperature during the initial fixation. At higher temperature the fixer works faster (Arrenius? rule - the speed of chemical reaction will increase twice for each 10oC, so 0-20oC difference gives you x4 increase) - so your tissue supposed to fixed faster. Again, at higher temperature the fixer penetration is faster as well. From another hand, these rules applied for every reaction in the tissue, so the speed of autolisis will increase as well. So, we don't really know what is the balance between fixation and autolisis.
Personally, I almost perform all procedures for liver/kidney/spleen on ice. I also used fresh ice-cold EM grade fixer. The time between animal death and taking sample should be no longer than a few minutes. I usually prepare everything well ahead: ice box, Petri dish with fixer, sharp new scalpel, tweezers. We are working in pair: one person taking care of animal, and me - samples for EM. Working in duet, I am able to take 3x3 mm piece of liver/spleen or whole kidney in about 40 seconds after animal's death. I immediately transfer tissue into the Petri Dish with fixer and cut it on 1x1x1 mm cubes (may be a little bit bigger, you have to do it quick and not so precise) under the fixer. When all samples had taken (about 20 min later), I moved samples into the fresh cold fixer and cut tissue on 0.5x0.5x0.5 cubes. Fixed them for 1-2 more hours on ice. In most cases I am using 1.5 or 2% GA in 1x PBS (20 mM Na-Phosphate, 150 mM NaCl, pH 7.4).
This procedure will work for most tissue, but brain. For brain, I am using room temperature fixer and perfusion. We are working in duet again. We perfused 1x PBS first (RT) to wash out the blood and fixer is next. After perfusion I perform all procedures on ice if possible.
To check fixation quality, look on the mitochondria: if crysties is not bubbled and membranes are parallel, the matrix does not have 'holes' and uniform - the fixation is OK. Another criteria: nucleolus membrane - if both membranes are parallel and tight, without big spaces/bubbles between, nuclear pores are pronounced - it's a good sign. Expanded/bubbled/non-uniform rER usually is indication of the hypoxia or some metabolism problems in the cell (not necessary fixation).
I hope it helps. Sergey
At 04:24 AM 8/7/02, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
I assumed from the phrasing that it was meant the capacity for infection after fixation. If I'm wrong someone please give me your opinion on this anyways because I'm curious. Thanks, Joshua Steindler
If your tissue has been fixed in glutaraldehyde then you will not have any infectious material. Glutaraldehyde is the "Agent of Choice" by hospitals when sterilizing surfaces and instruments after HIV infected patients have been treated. Never mind that the staff eventually develop sensitisation to the glut. Regards JVN
josh (by way of MicroscopyListserver) wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I assumed from the phrasing that it was meant the capacity for } infection after fixation. If I'm wrong someone please give me your } opinion on this anyways because I'm curious. } Thanks, } Joshua Steindler } } }
-- John V Nailon Executive Officer and Operations Manager The Centre for Microscopy and Microanlaysis The University of Queensland St Lucia QLD 4072 Tel: +61-7-33654214 Fax: +61-7-33654422 WWW: http://www.uq.edu.au/nanoworld
An issue to strike terror into the hearts of scanning electron microscopists !
In the period since our ESEM last received service attention in April we have had some very nasty crystals develop quite extensively on an upward facing aluminium surface low down in the column. Unfortunately the technician who made the discovery did not alert me in time to photograph the crystal growths - he was spared lynching only by the fact that he scraped about 5mg of the material into a vial. We have tried various methods of chemical analysis without anything definitive emerging, just aluminium and oxygen.
The only clue that we have is that it appears that a specimen containing high levels of fluoride had been examined several weeks earlier. I am not an excuse for a chemist but I am informed that fluorine gas combined with the water vapour semi vacuum of the ESEM could have been the cause.
Can anyone shed any light on this phenomenon and its possible cause ?
Thanks
Tony
Tony Bruton University of Natal Pietermaritzburg South Africa
The US Army Research Laboratory has advertised a TEM position. US CITIZENS ONLY. You may find the posting at: http://jsearch.usajobs.opm.gov/summary.asp?OPMControl=TS1801. If that page has expired, I got that information by searching for the announcement # (ACU201641) at http://www.usajobs.opm.gov/. Here is what I copied from that site. Please send your applications to the address in the advertisement and not to me.
Vacancy Announcement
USAJOBS Control No. TS1801 FO
www.USAJOBS.opm.gov, the U. S. Government's official source of job information, provides this information to the public at no cost.
Announcement No: ACU201641
Opening Date: August 07, 2002
Closing Date: August 20, 2002
Position Title (Pay Plan-Series): MATERIALS RESEARCH ENGINEER (-0806)
Grade: 03
Comments: THIS IS A DELEGATED EXAMINING ANNOUNCEMENT, OPEN TO ALL US CITIZENS. THIS VACANCY IS NOT COVERED UNDER RESUMIX PROCEDURES. IN ORDER TO BE CONSIDERED FOR THIS POSITION YOU MUST FOLLOW THE DIRECTIONS UNDER HOW TO APPLY AND SUBMIT THE PROPER FOR MS.
YOU MUST SUBMIT A SEPARATE APPLICATION AND ATTACHMENTS FOR EVERY JOB ANNOUNCEMENT YOU ARE APPLYING FOR. PLEASE MAKE SURE YOUR RESUME/APPLICATION CONTAINS THE JOB ANNOUNCEMENT NUMBER AND YOUR SOCIAL SECURITY NUMBER. YOU MUST INCLUDE THE ANNOUNCEMENT NU MBER ON ALL DOCUMENTS SUBMITTED.
TENURE: Permanent.
NOTES: (1) This is a career-conditional appointment. Career and career-conditional employees selected under this announcement will be required to serve an initial probationary period of up to three years. (2) You must follow the procedure stated in th e "How to Apply" section to receive consideration under this announcement. (3) Employee will be required to obtain and maintain a Secret security clearance. (4) Incumbent will be required to submit an annual Financial Disclosure statement.
The U.S. Army Research Laboratory is participating in an alternative personnel system known as the Personnel Demonstration Project. Among other features, the Demonstration Project replaced GS grade levels with occupational families and paybands. The s alary range of the position being filled will not be decreased with the change to paybands.
FILING DEADLINE: APPLICATIONS MUST BE RECEIVED BY THE CLOSING DATE. LATE APPLICATIONS WILL NOT BE CONSIDERED. Number of vacancies to be filled by this announcement : One (1).
Salary: $55,694
Region: Northeast
Organization: US Army Research Laboratory Sensors and Electron Devices Directorate Radio Frequency and Electronics Division
Duty Station: Adelphi, MD
Area of Consideration: Opened to all applicants with or without Civil Service Status.
Duties: Work involves research into improved microanalysis techniques for electronic devices and microanalysis of new device types. Expertise, knowledge, and skills include materials science, semiconductor device technology and sensitive analytical tec hniques as well as related areas such as semiconductor processing technology and device physics.
Serves as an engineer responsible for carrying out advanced research and development activities involving complex equipment, emerging technologies or scientific phenomena. The work involves research, development or systems analysis of new equipment, mat erial, or concepts that significantly add to the understanding and usefulness of previously unexplained or untested phenomena or contribute to the solution of significant Army problems. Is considered to be a productive professional, providing technical advice and guidance to managers, supervisors, peers, and sponsors on various aspects of the work. In many instances, experimental data are nonexistent or controversial requiring the incumbent to develop interpretations and procedures to extend existing knowledge/methodology. Is responsible for technically defending and supporting ideas and proposals for concepts that are often controversial or novel. Technical contributions are recognized by management and peers as having signific! ant impact on ongoing projects and reflect originality and creativity. Attends and presents papers at conferences or professional society meetings, serves on technical committees within the agency, and coordinates with other professionals when working o n collaborative efforts. Incumbent is skilled in applying a range of scientific principals, techniques and methods in a specialty area. Investigates problems of considerable complexity and finds non-obvious solutions. Results of work make a considerable contribution in resolving Army problems; advance scientific knowledge and understanding or capability; or overcome technical obstacles recognized by other professionals as highly complex. Conceives and formulates ideas or produces work of such original ity, soundness and value as to have marked the incumbent as a significant contributor to the field. Guides and evaluates the design and development activities of contractors and others in achieving new products. Uses complex theoret! ical, experimental and investigative techniques to resolve bot
Performs other duties as assigned.
Qualification Requirements: Basic Education Requirement for Engineers: A. Candidates must show successful completion of a full four-year professional engineering curriculum leading to a bachelors or higher degree in an accredited college or university. Acceptable curriculums must be accredited by the Accreditation Board for Engineering and Technology (ABET) or include mathematics and engineering science or physics courses in the required areas. OR B. Candidates may substitute for the above basic requirement at least four years of college-level education, training or technical experience that furnished a knowledge and understanding of engineering science and techniques equivalent to that provided by a full four-year professional engineering curriculum. (The adequacy of such background must be demonstrated by (1) professional registration, (2) written test (EIT) examination), (3) specific academic courses or (4) related curriculum).
NOTE: Foreign Education: Foreign education must be evaluated for U.S. equivalency in order to be rated eligible for this position. Please include this information either in your resume or by furnishing a copy of your certificate in your application package.
In addition to the Basic Requirements stated above, applicant must possess one year of specialized experience equivalent to the GS-11 grade level in the federal service.
SPECIALIZED EXPERIENCE: Specialized experience is experience which has equipped the applicant with the knowledge, skills and abilities (KSAs) necessary to successfully perform the duties of the position and is typically in or related to materials scienc e, semiconductor device technology and sensitive analytical techniques as well as related areas such as semiconductor processing technology and device physics.
Selective Placement Factors/Knowledge Skills and Abilities (KSA's): KNOWLEDGE, SKILLS AND ABILITIES (KSAs): Candidates will be rated on their possession of the following knowledge, skills, and abilities. Candidates must address each of the KSAs specifi cally on plain bond paper and submit it along with the other application materials. Information may include experience, education, training and awards as it relates to each KSA. Since you will be rated based on your possession of the KSAs listed in thi s announcement and a ranking determination made which affects your chances for employment, it would benefit you to provide your responses to the KSAs on a separate sheet of paper and submit it with your application.
KSA 1. Ability to perform research and development in electronic material characterization, growth and device characterization with direct working knowledge of Transmission Electron Microscopy (TEM), X-Ray Diffraction and Metal Organic Chemical Vapor D eposition (MOCVD) tools and processes.
KSA 2. Ability to perform research and development in electronic material characterization, growth and device characterization with general knowledge of other analysis techniques such as Scanning Electron Microscopy (SEM), Secondary Ion Mass Spectromet ry (SIMS), Auger Electron Spectrometry (AES), etc.
KSA 3. Ability to communicate in writing.
Standard/Other Requirements: 1. Failure to provide all of the required information as stated in the announcement may result in an ineligible rating or may affect the overall rating. 2. Incumbent is required to file an annual financial statement. 3. Permanent change of station (PCS) funds will be authorized. 4. Selection for this position is contingent upon proof of U.S. citizenship. 5. Direct Deposit is REQUIRED : As a condition of employment, candidates appointed, competitively promoted or reassigned are required to enroll and participate in Direct Deposit/Electronic Funds Transfer within 60 days following the effective date of t hat action. 6. Application/Resume deadline: Application/Resume must be received by the Closing Date of the Vacancy Announcement. 7. Male applicants born after December 31, 1959, are required to complete a Pre-Employment Certification Statement for Selective Service registration prior to appointment. Failure to comply may be grounds for withdrawal of an offer of employment, or di smissal after appointment. 8. HOW TO APPLY: Submit the following documents (Numbers 1-4) to the address listed under Where To Submit Package:
1. OF612, Optional Application for Federal Employment (this form can be found at www.opm.gov/forms/word/of612.doc, or a Resume. The resume may be typed or legibly handwritten and must contain, at a minimum: Announcement Number; Name; Address; Social Sec urity Number; Position Title and Grade of the job you are applying for; your paid/unpaid work experience including job title, duties and accomplishments, employers name and address, supervisors name and phone number, starting and ending dates (Month and Year), hours worked per week and grade/salary; education.
2. Separate sheet(s) of bond paper describing how your experience, education, training, awards relate to the Knowledge, Skills, and Abilities (KSAs) listed in this announcement. Since you will be rated based on your possession of the KSAs listed in this announcement and a ranking determination made which affects your chances for employment, it would benefit you to submit your responses to the KSAs along with your application. Since failure to do so would result in the examiner having less pertinent j ob-related information in which to evaluate you, a lower rating could result.
3. College Transcripts. NOTE: IF EDUCATION IS BEING USED IN LIEU OF EXPERIENCE, A COPY OF YOUR TRANSCRIPTS MUST BE PROVIDED. (If you are a current Federal employee holding a position requiring the same basic qualifications as the position for which you are applying, a Notification of Personnel Action (SF-50) will be accepted in lieu of transcripts.)
4. Applicants claiming veterans' preference must CLEARLY do so in their resume/application. Applicants claiming 5-point preference must include specific, detailed information in their resume/application which supports their claim for veterans' prefere nce, e.g., actual dates of service, type of duty (active, reservist), campaign badges or medals awarded, rank at time of retirement, etc. If information needed to verify entitlement to veterans preference is not provided in the resume/application, prefe rence will be denied. Applicants claiming 10-point preference MUST submit a DD Form 214 AND supporting documentation, e.g., Letter from VA dated within one year. Failure to submit supporting documentation will result in loss of consideration for 10-po int preference. If veterans preference is awarded and the applicant selected, a DD Form 214 (Member-4 copy) is required at the time of appointment to verify preference. Failure to provide the DD Form 214 at the time of appointment! will result in the offer of employment being withdrawn.
NOTE FOR MILITARY SPOUSES: Spouse preference eligibles must provide a copy of sponsors Permanent Change of Station (PCS) orders AND clearly state in their resume that they are requesting Military Spouse Preference.
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Without going into a long "dissertation" like others, it sounds like the standard corrosion we get from any aluminum parts that are near an ocean.
Many of my customers are less than 10 miles from the beach & I notice corrosion problems with relays, connectors as well as panel parts. Other environmental problems have been with the deterioration of rubber hoses in the LA area (years ago). I am informed that the smog causes the rubber to crack.
Hope this helps,
Earl
----- Original Message ----- } From: "Tony Bruton" {Bruton-at-nu.ac.za} To: {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, August 08, 2002 3:09 AM
Thanks to all that answered on and off the list!
The amount of advices that I got was far more than I ever hoped for. Armed with all these advices and instructions I feel confident that the move will go without problems.
I also am sorry that I wrote the part about the Swedish JEOL office, I have only got positive comments about the service you get from JEOL, so I will give it a second try to see if they have any advices. Maybe I just got the wrong guy on the phone or I call at a bad time. One occasion isn't enough for forming an opinion and I should be old enough to know that. Anyhow, I have to give them credit to supporting this instrument for the last 20 years, because it's in perfect condition as far as I could see.
I will report back to the list when I have installed it and put it all together.
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on 8/6/02 12:44 PM, Chris Jeffree at cjeffree-at-srv0.bio.ed.ac.uk wrote:
Dear Ruchika and Chris,
} no microscope available can cover a } field as large as 20mm in a single image at 500x. } The image would be 1 metre wide!
Worse! The image would be 10 m wide.
} A field width of 0.25 x 0.20 mm would be nearer the mark - } if the final image magnification is } 500 times when enlarged to fit onto 5"x4" film. } } Many modern microscopes use digital cameras, } and there are several software } packages that can stitch multiple images together } to cover larger areas than the microscope's limited field width. } However, at 500x you would need to stitch several } thousand images together (at 0.25x0.2mm, 80x100=8000 images), } and the final file size } (1 image = minimum 1Mb: 8000 images =8Gb)would defeat } all but the most powerful computers. Certainly not a } job for the old PC. Well, not mine anyway! } In addition, someone would have to check the alignment to make sure that the images had been stitched together properly, since, at the rate of 1 image per second, it will take over 2 hrs just to collect the images, and temperature variations over the 20 mm field during that time can cause an automated stitching program to give poor results. Yours, Bill Tivol
If you have a light element EDS detector, you should be able to see fluorine if present in the company of aluminum. F should be detectable if it reacted with the Al (unless that compound is not stable in its environment and the F was lost as a gas in a continuing reaction of some sort???). Going on memory (did not look-up), aluminum fluoride is rather reactive itself. Maybe a chemist can expound on that...
When aluminum reacts in an aqueous environment it typically forms a white powder (aluminum oxide/hydroxide).
This would be a highly unusual situation for a high vacuum system, but not so surprising for a variable pressure system using water vapor.
Woody White McDermott Technology Inc.
http://woody.white.home.att.net
-----Original Message----- } From: Tony Bruton [mailto:Bruton-at-nu.ac.za] Sent: Thursday, August 08, 2002 6:10 AM To: Microscopy-at-sparc5.microscopy.com
Greetings all
An issue to strike terror into the hearts of scanning electron microscopists !
In the period since our ESEM last received service attention in April we have had some very nasty crystals develop quite extensively on an upward facing aluminium surface low down in the column. Unfortunately the technician who made the discovery did not alert me in time to photograph the crystal growths - he was spared lynching only by the fact that he scraped about 5mg of the material into a vial. We have tried various methods of chemical analysis without anything definitive emerging, just aluminium and oxygen.
The only clue that we have is that it appears that a specimen containing high levels of fluoride had been examined several weeks earlier. I am not an excuse for a chemist but I am informed that fluorine gas combined with the water vapour semi vacuum of the ESEM could have been the cause.
Can anyone shed any light on this phenomenon and its possible cause ?
Thanks
Tony
Tony Bruton University of Natal Pietermaritzburg South Africa
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I certainly agree with you. Although HIV can develop drug resistance, it is not that omni-resistant. As I mentioned in the other note, we raised that issue at Harvard way back in early 1989 and did a primitive test of several common disinfectant agents. Most of them, including aldehyde fixative and ethanol, can effectively kill these viruses. The test was never published since we thought it was just a simple "observation" and was only intended for better lab practice. But it is important that we should remind ourselves about the potential risk. Regards,
QCY
At 03:01 PM 8/8/2002 +1000, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Qian-Chun Yu, MB, Ph.D. Director Cell Imaging Core Abramson Cancer Research Institute University of Pennsylvania 421 Curie Boulevard, Room 532 Philadelphia, PA 19104
Bill You're right, of course - my abacus needs servicing! Chris
} Worse! The image would be 10 m wide. } } } A field width of 0.25 x 0.20 mm would be nearer the mark - } } if the final image magnification is } } 500 times when enlarged to fit onto 5"x4" film. } } } } Many modern microscopes use digital cameras, } } and there are several software } } packages that can stitch multiple images together } } to cover larger areas than the microscope's limited field width. } } However, at 500x you would need to stitch several } } thousand images together (at 0.25x0.2mm, 80x100=8000 images), } } and the final file size } } (1 image = minimum 1Mb: 8000 images =8Gb)would defeat } } all but the most powerful computers. Certainly not a } } job for the old PC. Well, not mine anyway! } } } In addition, someone would have to check the alignment to make sure that } the images had been stitched together properly, since, at the rate of 1 } image per second, it will take over 2 hrs just to collect the images, and } temperature variations over the 20 mm field during that time can cause an } automated stitching program to give poor results. } Yours, } Bill Tivol } }
Since we are on the topic of corrosion & EM, I would like to know if anyone near a body of salt water has noticed pitting on front surface coated mirrors in "optical" microscopes. To be specific, the type that uses visible light and the light is detected by the electromagnetic sensors symmetrically spaced about our noses.
I've never been in a lab. that was so close to an ocean that I can attribute any corrosion I've ever seen from salt air but I'd like to hear from anyone using optical tables [lasers/mirrors] or optical microscopes that knows this is a problem, and if shown to be, what is the solution? The only issue I have ever had with front surface coated mirror/optics is human fingerprints etching the surface or wet chemistry vapors in a fume hood doing the same.
Regards,
Peter Tomic Anadigics, Inc.
-----Original Message----- } From: Earl Weltmer [mailto:earlw-at-sbcglobal.net] Sent: Thursday, August 08, 2002 10:59 AM To: Tony Bruton; Microscopy-at-sparc5.microscopy.com
Hi Tony,
Without going into a long "dissertation" like others, it sounds like the standard corrosion we get from any aluminum parts that are near an ocean.
Many of my customers are less than 10 miles from the beach & I notice corrosion problems with relays, connectors as well as panel parts. Other environmental problems have been with the deterioration of rubber hoses in the LA area (years ago). I am informed that the smog causes the rubber to crack.
Hope this helps,
Earl
----- Original Message ----- } From: "Tony Bruton" {Bruton-at-nu.ac.za} To: {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, August 08, 2002 3:09 AM
Thank you to everyone who replied - many privately. I did mean capacity for infection, not ultrastructure. All who replied stated that infection was impossible, HIV being quite a fragile beastie.
Cheers,
Diana
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
--
Diana van Driel Department of Clinical Ophthalmology Sydney University GPO Box 4337 Sydney NSW AUSTRALIA 2001
You should attempt to excavate some material from the corrosion pit that undoubtedly resides at the point of attachment of one of these crystals. A fine tungsten needle works well for the job. Analyze what you extract and look for halogens and transition metals.
A likely cause is that fine particles of some transition metal halide landed on the aluminum and established a galvanic cell whereby the metal was reduced and aluminum halide was produced as a transitory product. Conversion of the aluminum halide to aluminum hydroxide liberates the halogen so that it re-enters the catalytic cycle of attack on the aluminum.
The seemingly stable silver chloride will provide a dramatic demonstration of this. A flake of silver chloride in contact with aluminum, even heavily anodized aluminum, will often result in run-away pitting such that the next morning a puddle of deliquescent aluminum chloride mixed with aluminum hydroxide "curd" remains where the chip was. In the high vacuum environment of the SEM the process would be slower and more likely result in a solid product.
A very small amount of chloride may be responsible for a much larger volume of corrosion because it is not consumed in the final products.
John Twilley Conservation Scientist
Tony Bruton wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Greetings all } } An issue to strike terror into the hearts of scanning electron } microscopists ! } } In the period since our ESEM last received service attention in April } we have had some very nasty crystals develop quite extensively on an } upward facing aluminium surface low down in the column. Unfortunately } the technician who made the discovery did not alert me in time to } photograph the crystal growths - he was spared lynching only by the fact } that he scraped about 5mg of the material into a vial. We have tried } various methods of chemical analysis without anything definitive } emerging, just aluminium and oxygen. } } The only clue that we have is that it appears that a specimen } containing high levels of fluoride had been examined several weeks } earlier. I am not an excuse for a chemist but I am informed that } fluorine gas combined with the water vapour semi vacuum of the ESEM } could have been the cause. } } Can anyone shed any light on this phenomenon and its possible cause ? } } Thanks } } Tony } } Tony Bruton } University of Natal } Pietermaritzburg } South Africa
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For a multi-faceted project involving many researchers, I wanted to document a polished sulfide surface for spacial notations and sharing ... I thought the least I could do is scan the surface with a flatbed. As those who may have tried this know, the result is very dark because the lamp & sensor are not in close enough angular alignment. This was a disappointment, because I wanted to try modifying the scanner with a polarized sheet of mylar.
My question for the list: Do I rule out ALL flatbed scanners for this? Has anyone encountered a scanner for which the alignment is close enough for measuring mirror-like (and anisotropic) relectances??
cheerios ... shAf :o) Avalon Peninsula, Newfoundland www.micro-investigations.com (in progress)
The responses from Sergey and Drew are pretty good and complete- follow them and you will be fine.
What exactly is broken in the OL current control? Is that one of the focusing switches/potentiometers at the front panel, or an OL control circuit itself? Please clarify, maybe we can find a replacement, or suggest a substitute.
It is surprising to hear that JEOL is not helpful. They are very good here in USA. Try again- perhaps they were just overworked at the moment...
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax (678)467-0012 mobile
This message is made of 100% recycled electrons. ----- Original Message ----- } From: Göran Axelsson {axelsson-at-acc.umu.se} To: {microscopy-at-sparc5.microscopy.com} Sent: Tuesday, August 06, 2002 6:36 PM
Hello list,
I'm looking for advices on how to move a Jeol JEM 100CX TEM.
I got a Jeol JEM 100CX this spring if I only moved it myself. After a lot of problems I finally have found a suitable place to put it in, so the next step is to move it. I will probably start picking it apart in 1-2 weeks time. The only problem is that I have never done it before and I don't have the budget to hire a technician to do it for me.
I'm doing this as a private hobby project to learn about EM, vacuum technology and just for fun. I really hate seeing good old instruments being scrapped. :-)
I'm not all by my self. I have a number of friends that have offered to help with all from repairing the electronics (the OL-current control is broken) to actually help lifting and driving the truck.
The plan is to use common sense, the mechanical drawings of the column and a digital camera to document every step when we take it apart. Then we might have a fighting chance to put it back again. I have talked with the Swedish representant of Jeol but they claimed they didn't have any documentation on how to move or put it together. They sounded so negative so I don't think I have much help to get there. Maybe they discovered that I wasn't a customer with a fat wallet. :-)
As I have learnt a lot from this list by lurking and reading the archives, (Thanks Nestor!) I now try to get some advice to how I should move the TEM. And more importantly, what I should avoid to do! I'm happy for any advice of horror story you want to share. If you have some documents to share, I could pay for copying cost and postage, just let me know.
Whatever the result is I haven't payed much for it, so if I fail to get it running again I will probably have learnt a lot and if I get it running I also get a TEM. I just can't loose! :-)
The diary and details of my EM adventure is documented on http://www.home.neab.net/gandalf/EM-lab/index.htm Anyone wanting to see the inside if the OL-current control? Just follow the link.
If you read this far you are either laughing or just shaking your head. Thanks anyhow.
Whoa. HIV is NOT infective after glutaraldehyde fixation.
I thought the original question was how to preserve it's ultrastructure for study. It's "morphology" looks just fine after routine fixation. It's "transmissibility" does not survive fixation. It is a conventional virus containing RNA and protein that are effectively cross-linked and inactivated by fixation.
On the other hand, prions (proteinaceous filaments not known to contain nucleic acids) are not killed by routine fixation. As a matter of fact, even routine autoclaving does not inactivated them. Killing them requires high heat for a long time, or chemical treatments like hydroxide, or formic acid.
Prions cause spongiform encephalopathies (e.g., Bovine Spongiform Encephalopathy (BSE or mad cow disease), Creutzfeld-Jacob Disease (CJD), scrapie, chronic wasting disease of deer/elk, and others). Thus, neural tissue should be handled with care.
I hope this clears up the question.
Sara
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Apologies for going off-topic, but I have a question for all you corrosion experts out there.
Will pure Au undergo grain boundary corrosion in a hot (100C) damp atmosphere? If not, would the presence of atomic nitrogen and/or fluorine on the surface make a difference?
I always ignorantly assumed that Au didn't corrode at all but I've just seen some which definately has.
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Michael, By default scanners work by indirect or "darkfield" illumination so there is no good way to scan by direct reflection. The only possible way this would work is to tilt the sample relative to the platen and by trial and error you may get a correct angle. The problem with this is that most of your sample will be above the platen and probably out of focus. I have no idea of the depth of field of a typical scanner. Russ Gillmeister Xerox ~~~~~~~~~~~~~
-----Original Message----- } From: michael shaffer [mailto:rarewolf-at-roadrunner.nf.net] Sent: Friday, August 09, 2002 8:37 AM To: MicroscopyList
For a multi-faceted project involving many researchers, I wanted to document a polished sulfide surface for spacial notations and sharing ... I thought the least I could do is scan the surface with a flatbed. As those who may have tried this know, the result is very dark because the lamp & sensor are not in close enough angular alignment. This was a disappointment, because I wanted to try modifying the scanner with a polarized sheet of mylar.
My question for the list: Do I rule out ALL flatbed scanners for this? Has anyone encountered a scanner for which the alignment is close enough for measuring mirror-like (and anisotropic) relectances??
cheerios ... shAf :o) Avalon Peninsula, Newfoundland www.micro-investigations.com (in progress)
on 8/8/02 10:09 PM, Peter Tomic at PTomic-at-anadigics.com wrote: } } Since we are on the topic of corrosion & EM, I would like to know if anyone } near a body of salt water has noticed pitting on front surface coated } mirrors in "optical" microscopes. To be specific, the type that uses } visible light and the light is detected by the electromagnetic sensors } symmetrically spaced about our noses. } } I've never been in a lab. that was so close to an ocean that I can attribute } any corrosion I've ever seen from salt air but I'd like to hear from anyone } using optical tables [lasers/mirrors] or optical microscopes that knows this } is a problem, and if shown to be, what is the solution? The only issue I } have ever had with front surface coated mirror/optics is human fingerprints } etching the surface or wet chemistry vapors in a fume hood doing the same. } Dear Peter, I don't know about salt air, but, when I was an undergraduate, there was a 1/10 scale model of the Palomar telescope whose (1st-surface Al) mirror was severely degraded by the ambient smog. Combined with the--fortunately temporary--degradation of the electromagnetic sensors, such as you describe, the overall performance was reduced by a couple of orders of magnitude. The only solution was to recoat the mirror frequently, but, if the mirror components are small enough, I'd think that storing them in a desiccator when not in use would improve the lifetime. A little activated charcoal might help with the smog, and a desiccant could help with the salt, unless it was in the form of dry dust--which might not induce corrosion. Since smog is more-or-less everywhere these days, it could easily be a more serious problem than salt air. Yours, Bill Tivol
Just an aside, but a curious one, to the recent discussions on corrosion.
I had a problem surface a couple of years ago in a couple of similar vintage, older SEMs (around 25 years old). The lenses used in the cameras, to image the record CRT on to the film, had developed serious pitting over the years. The only thing that I have been able to come up with is that the sulfide bearing negative fixing solutions were always kept near the camera assembly. The lenses were originally coated, but cleaning over the years had left scratches in the coatings, and the pitting clearly occurred along these scratches. Bare aluminum surfaces in the assembly also showed corrosion.
With more digital imaging these days, this will be less of a problem. But sometimes, given enough time, the most unexpected sources can cause problems.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174
} From: "Bill & Sue Tivol" {wtivol-at-earthlink.net} : on 8/8/02 10:09 PM, Peter Tomic at PTomic-at-anadigics.com wrote: : } : } Since we are on the topic of corrosion & EM, I would like to know if anyone : } near a body of salt water has noticed pitting on front surface coated : } mirrors in "optical" microscopes. To be specific, the type that uses : } visible light and the light is detected by the electromagnetic sensors : } symmetrically spaced about our noses. : } : } I've never been in a lab. that was so close to an ocean that I can attribute : } any corrosion I've ever seen from salt air but I'd like to hear from anyone : } using optical tables [lasers/mirrors] or optical microscopes that knows this : } is a problem, and if shown to be, what is the solution? The only issue I : } have ever had with front surface coated mirror/optics is human fingerprints : } etching the surface or wet chemistry vapors in a fume hood doing the same. : } : Dear Peter, : I don't know about salt air, but, when I was an undergraduate, there was : a 1/10 scale model of the Palomar telescope whose (1st-surface Al) mirror : was severely degraded by the ambient smog. Combined with the--fortunately : temporary--degradation of the electromagnetic sensors, such as you describe, : the overall performance was reduced by a couple of orders of magnitude. The : only solution was to recoat the mirror frequently, but, if the mirror : components are small enough, I'd think that storing them in a desiccator : when not in use would improve the lifetime. A little activated charcoal : might help with the smog, and a desiccant could help with the salt, unless : it was in the form of dry dust--which might not induce corrosion. Since : smog is more-or-less everywhere these days, it could easily be a more : serious problem than salt air.
A gold coating over the aluminum of the mirrors should protect them from corrosion for a longer period of time it the gold coating did not cause unacceptable changes in the reflected light due to the gold forming a non linear dielectric mirror. If the gold coating was very thin the problems should be acceptable for most things.
Gordon Gordon Couger gcouger-at-couger.com
I collect links on information related to light microscopes. http://www.couger.com/microscope/links/gclinks.html Please forward any links or information you think might be useful to others.
I'm not a corrosion expert and I'd love to hear a good definition of corrosion from a metallurgist, chemist, quantum physicist or whomever. However, I do know that Au is "corroded" and/or etched by the halides F-, Cl-, Br-, and I. I routinely use aqueous potassium iodide to strip Au metallization from GaAs microcircuits as opposed to "aqua regia" for failure analysis purposes. Cl is particularly aggressive. Etch rate for KI is about 20 Angstroms/sec. but will vary with density of the film and graininess.
Try this experiment. Place one of your devices that is not passivated with SiN, SiO2 or anything with exposed metal in a closed container with a common bleach that contains chlorine. You will see how aggressive Cl is to that gold surface and you may look at the effect on an SEM. It also tends to blacken the surface. I would imagine this is due to the pitting but have never had a reason to look deeply into it.
There is bromine in plastic mold compound material used in most commercial grade integrated circuits and is there as a flame retardant. The volume is low, ~ 15 PPM, but may not be evenly distributed throughout the plastic compound so high concentrations can exist in small regions of the compound. It also creates problems in Al interconnects.
If you are finding Au corrosion to be a reliability issue in your devices, please talk to me off-line.
Regards,
Peter Tomic Anadigics, Inc. Warren, New Jersey U.S.A.
-----Original Message----- } From: Richard Beanland [mailto:richard.beanland-at-bookham.com] Sent: Friday, August 09, 2002 1:06 PM To: 'Microscopy-at-MSA.Microscopy.Com'
Apologies for going off-topic, but I have a question for all you corrosion experts out there.
Will pure Au undergo grain boundary corrosion in a hot (100C) damp atmosphere? If not, would the presence of atomic nitrogen and/or fluorine on the surface make a difference?
I always ignorantly assumed that Au didn't corrode at all but I've just seen some which definately has.
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This sounds like the classic example of silver halide that I described in my previous email on this question. I don't know about the corrosion behavior of aluminum exposed to thiosulfate (the fixer is not normally a sulfide) or sulfur dioxide (gas released by oxidation of the fixer) but the silver chloride / silver bromide of the image layer is certainly going to corrode the aluminum if particles come off on it.
The same thing is a problem in X-ray diffraction labs that use powder cameras. Normally one uses a punch to create holes in a strip of film for the X-ray entry and exit collimators. The cutting edge of the hole punch routinely corrodes due to being driven into the silver halide emulsion and can begin to shed rust particles on the film, giving rise to annoying spots.
John Twilley
Allen Sampson wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Just an aside, but a curious one, to the recent discussions on corrosion. } } I had a problem surface a couple of years ago in a couple of similar } vintage, older SEMs (around 25 years old). The lenses used in the cameras, } to image the record CRT on to the film, had developed serious pitting over } the years. The only thing that I have been able to come up with is that } the sulfide bearing negative fixing solutions were always kept near the } camera assembly. The lenses were originally coated, but cleaning over the } years had left scratches in the coatings, and the pitting clearly occurred } along these scratches. Bare aluminum surfaces in the assembly also showed } corrosion. } } With more digital imaging these days, this will be less of a problem. But } sometimes, given enough time, the most unexpected sources can cause } problems. } } Allen R. Sampson } Advanced Research Systems } 317 North 4th. Street } St. Charles, Illinois 60174 } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com }
Hi - does anyone have ideas how to quantify metal distributions (Mn, Fe, Ni, Zn) on MnOx within biofilms? I am dealing with Mn oxidizing bacteria in an acid-mine drainage contaminated creek in AZ. I would like to be able to distinguish between the bacteria and algae that are present on glass slides put out in the creek, if possible, as well as determine the distributions of the metals (i.e., do they overlay the bacteria?). I am considering using the LIVE/DEAD BacLight kit from M.Probes w/ CSLM, but have heard that it stains ALL cells including protozoa, algae, fungi etc. Is there something that would stain bacteria only such as DAPI or acridine orange, and then couple that with something to detect the metals? I don't think there is a probe availabe to detect metals associated with MnOx. It seems like it may be a multi-step process, and not something that can be accomplished with just one microscopy technique.
Thanks,
Hanna Gilbert Hydrology and Water Resources University of Arizona
took the green filter out of a FITC filter block took the objective off the microscope put a dish of GFP expressing yeast or bacteria on the microscope zapped with blue light from the FITC filter block held the green filter up to our eyes like a monocle
This worked fine to see which areas of the plate were GFP positive and which were negative.
At 02:47 PM 7/19/2002 +1000, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jason-clark-at-uiowa.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, August 12, 2002 at 15:08:36 ---------------------------------------------------------------------------
Email: jason-clark-at-uiowa.edu Name: Jason Clark
Organization: University of Iowa
Education: Graduate College
Location: Iowa City, IA
Question: I am trying to visualize a sodium channel that has a short half life (~40 min) at the cell membrane. There may be a large pool of this channel intracellularly (maybe for recycling) and we are trying to distinguish between the two populations. What is the best way to view the membrane bound population while excluding the intracellular population?
Hi, Before I buy another copy of Adobe PhotoShop, I wanted to see what else people were using to handle their TEM data. Adobe is great for its versatility but is there another package that matches this versatility and that is better suited to microscopy (i.e. having a feature that allows one to measure the length of features, read pixel values, or generate gray-level line traces)? Alternatively, are there plug-ins available for Adobe that let you perform these tasks?
In a message dated 8/12/02 4:21:55 PM, David.Mathes-at-honeywell.com writes:
} Before I buy another copy of Adobe PhotoShop, I wanted to see what else } people were using to handle their TEM data. Adobe is great for its } versatility but is there another package that matches this versatility } and that is better suited to microscopy (i.e. having a feature that allows } one to measure the length of features, read pixel values, or generate gray-level } line traces)? Alternatively, are there plug-ins available for Adobe that } let you perform these tasks?
There is a complete set of Photoshop plugins that provide the kinds of processing and measurement tools appropriate for microscopy. Go to ReindeerGraphics.com and check out Fovea Pro (there is also an online tutorial that shows some of the functionality).
there are a number of alternative software packages available. Please check out our web site for more information on our product (analySIS). Other TEM-specific packages are for example from EmiSpec. Calibration, measurement, pixel values, line traces, report generation, etc. are all integrated part of the software.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
Disclaimer: We produce and sell the analySIS family of image analysis software, and therfore have considerable interest in the product. We are NOT affiliated with other software mentioned in this email.
-----Original Message----- } From: Mathes, David (TX95) [mailto:David.Mathes-at-honeywell.com] Sent: Monday, August 12, 2002 2:08 PM To: 'microscopy-at-sparc5.microscopy.com'
Hi, Before I buy another copy of Adobe PhotoShop, I wanted to see what else people were using to handle their TEM data. Adobe is great for its versatility but is there another package that matches this versatility and that is better suited to microscopy (i.e. having a feature that allows one to measure the length of features, read pixel values, or generate gray-level line traces)? Alternatively, are there plug-ins available for Adobe that let you perform these tasks?
As far as I know Fovea Pro is a set of plugins for Photoshop, which helps you with some of the image processing you want to do. Dr. John Russ is involved in the development of this software.
} -----Original Message----- } } From: Mathes, David (TX95) [mailto:David.Mathes-at-honeywell.com] } Sent: Monday, August 12, 2002 2:08 PM } To: 'microscopy-at-sparc5.microscopy.com' } Subject: software } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi, } Before I buy another copy of Adobe PhotoShop, I wanted to see what else } people were using to handle their TEM data. Adobe is great for its } versatility but is there another package that matches this versatility and } that is better suited to microscopy (i.e. having a feature that allows one } to measure the length of features, read pixel values, or generate gray-level } line traces)? Alternatively, are there plug-ins available for Adobe that } let you perform these tasks? } } Dave
Dear all, I have used the program CrystalKit for building supercells for the simulation of HREM images of GBs and planar defects. Is there any competition to this program, or is really the only package worth considering for this purpose.
} Does anyone know what the approximate attendance was at M&M in Quebec. } } Greg
Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, ICBR EM CORE University of Florida Ph. 352-392-1295 PO Box 118525 Fax 352-846-0251 Gainesville, FL 32611 http://www.biotech.ufl.edu/EM
I would like to purchase to following item for my coater: specimen holder, carbon thread or rod evaporator, arm complete and the 2 MC cables. Please contact me if you have these items or know where I might be able to purchase them. Thanks.
Phoebe J. Doss Manager/Adjunct Instructor Electron Microscope Lab Department of Physiological Sciences 264 McElroy Hall Oklahoma State University Stillwater, OK 74078 405-744-6765
We use Paintshop Pro from Jasc Software in our lab for image editing for light microscopy and SEM. I have been very pleased with its performance. It offers most of the functionality of Photoshop and is compatible with photoshop plug-ins. We use the Fovea Pro plug ins for image analysis and have also been pleased with them for routine measurements. -- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
} } Hi, } } Before I buy another copy of Adobe PhotoShop, I wanted to see what else } } people were using to handle their TEM data. Adobe is great for its } } versatility but is there another package that matches this versatility and } } that is better suited to microscopy (i.e. having a feature that allows one } } to measure the length of features, read pixel values, or generate gray-level } } line traces)? Alternatively, are there plug-ins available for Adobe that } } let you perform these tasks? } } } } Dave
Good day to all on the listserver, I have a question - we are currently having some problems with tissue being extremely autolyzed. What we have found out is that the person sending us the tissue uses premade Trumps fixative - they don't check the pH nor the osmolarity and the fixative comes to them unrefrigerated. I'm not sure how long they keep the fixative once they get it but I imagine it is longer than a week - could the wrong pH, hot temperature during shipment, and long storage be our only problem here? Supposably, they collect the samples within a reasonable time following exsanguination of the animal - I think bleeding has something to do with also - does anyone out there have suggestions or comments that could help us? Thanks in advance Connie A. Cummings, DVM, Phd
ImageJ provides all the functionality you mention - and much more. If you can program in Java, the versatility of this program exceeds almost any other imaging application. It is free and can bee obtained at http://rsb.info.nih.gov/ij/docs/index.html However, if you are doing batch processing involving a large number of images, you'll probably find it too slow.
"[ImageJ] runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine."
"ImageJ was designed with an open architecture that provides extensibility via Java plugins. Custom acquisition, analysis and processing plugins can be developed using ImageJ's built in editor and Java compiler. User-written plugins make it possible to solve almost any image processing or analysis problem."
Cheers, Paul Baggethun Pittsburgh, PA
-----Original Message----- } From: Mathes, David (TX95) [mailto:David.Mathes-at-honeywell.com] Sent: Monday, August 12, 2002 4:08 PM To: 'microscopy-at-sparc5.microscopy.com'
Hi, Before I buy another copy of Adobe PhotoShop, I wanted to see what else people were using to handle their TEM data. Adobe is great for its versatility but is there another package that matches this versatility and that is better suited to microscopy (i.e. having a feature that allows one to measure the length of features, read pixel values, or generate gray-level line traces)? Alternatively, are there plug-ins available for Adobe that let you perform these tasks?
I heard about 1500 (without vendors). About same as last year. I only heard this through the grapevine, no guarantees.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Greg Erdos [mailto:gwe-at-biotech.ufl.edu] Sent: Tuesday, August 13, 2002 10:27 AM To: Microscopy-at-sparc5.microscopy.com
} Does anyone know what the approximate attendance was at M&M in Quebec. } } Greg
Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, ICBR EM CORE University of Florida Ph. 352-392-1295 PO Box 118525 Fax 352-846-0251 Gainesville, FL 32611 http://www.biotech.ufl.edu/EM
There use to be several companies that sold lectins conjugated to colloidal gold. Does anyone know of a company that's still selling them? I appreciate any advice on this matter. Thanks, Beth Richardson
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) **********************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lillianft-at-aol.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, August 13, 2002 at 15:24:37 ---------------------------------------------------------------------------
Email: lillianft-at-aol.com Name: L. Domenico
Organization: CSE
Education: Undergraduate College
Location: City, State, Country
Question: I would like to use HMDS instead of using a critical point dryer for an SEM course I will be teaching. Experiments that HMDS is to be used is with plant parts, yeast, bacteria and animal tissue (mouse jejunum). Are there simple protocols used for HMDS for each of the specimens mentioned above.Thanks
You can get a "generic" carbon evaporation fixture from Ladd Research. www.laddresearch.com, catalog chapter 11. I don't know if it will fit your system. No commercial endorsement implied.
David Rothbard Earthborn Solutions rothbardD-at-netscape.net
pjdoss-at-cvm.okstate.edu wrote:
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Listers:
I would like to purchase to following item for my coater: specimen holder, carbon thread or rod evaporator, arm complete and the 2 MC cables. Please contact me if you have these items or know where I might be able to purchase them. Thanks.
Phoebe J. Doss Manager/Adjunct Instructor Electron Microscope Lab Department of Physiological Sciences 264 McElroy Hall Oklahoma State University Stillwater, OK 74078 405-744-6765
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Listers, We are trying to locate an antibody against elastin to use with sheep cartilage. We would prefer a poly-clonal if possible since we are looking for both unpolymerized and polymerized elastin. I would appreciate any leads as to where we can obtain this.
Thanks, Debby
Debby Sherman Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building West Lafayette, IN 47907
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id JAA10084 for dist-Microscopy; Wed, 14 Aug 2002 09:35:29 -0500 (CDT) Received: from njz_spm_filter (sparc5 [206.69.208.10]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id JAA10080 for "MicroscopyFilteredEmail1-at-msa.microscopy.com"; Wed, 14 Aug 2002 09:34:58 -0500 (CDT) Received: from mercury.uwe.ac.uk (mercury.uwe.ac.uk [164.11.132.23]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with ESMTP id JAA10073 for {Microscopy-at-sparc5.microscopy.com} ; Wed, 14 Aug 2002 09:34:46 -0500 (CDT) Received: from fas-fas533.uwe.ac.uk ([164.11.149.117]) by mercury.uwe.ac.uk (2.0.4/SMS 2.0.4-devel) with SMTP id PAA28830 for {Microscopy-at-sparc5.microscopy.com} ; Wed, 14 Aug 2002 15:29:21 +0100 (BST)
I have just enjoyed looking at the 2000 proceedings. Could someone post the volume and supplement no. for the 2001 proceedings?
Dave
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
The simplest starting point is to dehydrate to 100% ethanol as usual, keeping to the normal times. Then we use: ethanol:HMDS 2:1 ethanol:HMDS 1:2 3 washes in 100% HMDS
Remove from HMDS and leave to dry in fume cupboard. If too small, eg bacteria, let a drop dry on a coverslip.
Dave
On Tue, 13 Aug 2002 19:51:25 -0500 by way of Ask-A-Microscopist {lillianft-at-aol.com} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (lillianft-at-aol.com) from } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, } August 13, 2002 at 15:24:37 } --------------------------------------------------------------------------- } } Email: lillianft-at-aol.com } Name: L. Domenico } } Organization: CSE } } Education: Undergraduate College } } Location: City, State, Country } } Question: I would like to use HMDS instead of using a critical point } dryer for an SEM course I will be teaching. Experiments that HMDS is } to be used is with plant parts, yeast, bacteria and animal tissue } (mouse jejunum). Are there simple protocols used for HMDS for each } of the specimens mentioned above.Thanks } } --------------------------------------------------------------------------- }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
We have a Durst Laborator SM 183 enlarger. Does anyone have a list of lens configurations for the SM 183? We want to produce nice 8x10 electron micrographs but nothing we've come up with is satisfactory (ie. spherical aberration and uneven illumination). All of the lens configurations we have are for a 138 model. I believe we have objective lenses 180mm, 105mm, and 80mm and condenser lenses 240PT, 240, 200, 130, and 85.
I would really appreciate some help.
Thank you,
Jaclynn M. Lett, Research Technician Harold W. Siebens Hearing Research Center Central Institute for the Deaf 4560 Clayton Ave. St. Louis, MO 63110
InBios International 562 First Avenue South, Suite #600 Seattle, WA 98104 Phone: 206.344.5821 Fax: 206.344.5823
No financial interest in InBios.
Good luck,
Soumitra
************************************************************* Soumitra Ghoshroy Ph.D. Director, Electron Microscopy Lab Graduate Faculty, Department of Biology Box 3EML New Mexico State University Las Cruces, NM 88003 Tel: 505-646-3268 (office), 646-3283 (lab) Fax: 505-646-3282 e-mail:sghoshro-at-nmsu.edu URL:http://confocal.nmsu.edu/eml
On Tue, 13 Aug 2002, Beth Richardson wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } There use to be several companies that sold lectins conjugated to colloidal } gold. Does anyone know of a company that's still selling them? } I appreciate any advice on this matter. Thanks, } Beth Richardson } } ********************************************************************** } Beth Richardson } EM Lab Coordinator } Plant Biology Department } University of Georgia } Athens, GA 30602-7271 } } Phone - (706) 542-1790 & FAX - (706) 542-1805 } } "Between the two evils, } I always pick the one I never tried before". Mae West (1893-1980) } ********************************************************************** } } "And it's only the giving that makes you what you are". } Wond'ring Aloud, Jethro Tull (Aqualung) } } *************************************************************************** } } } }
Hi folks, I am looking for an EM facility within a few hundred miles of Dallas TX that would be willing to rent out time on their equipment to industry. Specifically, I would like access to a FIB and a TEM (preferably 200keV or better). Thanks Dave
Our scope starts to behave badly above 29 deg. C or so.
Ron L ----- Original Message ----- } From: "ATC SEM Laboratory" {atcsem-at-earthlink.net} To: {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, August 14, 2002 11:31 AM
I'm assuming you are asking about SEM equipment rather than protocols, specimens, etc.
Different manufacturers have their own set of environmental specs. I would presume that these are known-good ranges where no problems would occur. But each maker's units, and perhaps each unit, would have different max limits. If the SEM is computer controlled or even has processors inside, that is another dimension besides issues of regular electronics. Then, one has to consider whether the lens coils and/or HV supply are water cooled. The lens drivers are, as far as I know, always air cooled. Same for all the other electronics.
I run my SEM at room conditions between 68-72F, 25-38% RH. One time, when my A/C was dirty, temp got to 82F. SEM still worked. Here in CA, with the iffy power, early last year I installed two 6KVA double conversion UPS units--one for console, one for column. These run the system for about two hours with the console off. The issue was to keep the Zr/W FE gun running during power outages. The down side is that these UPS units increase heat load in the SEM room since they are in the same room. So, if the A/C were to fail, room temp would rise dramatically.
Nevertheless, I have not reached an upper temperature where the system quit working. And definitely not a limit where the system broke. So to answer your question-- it depends.
gary
At 08:31 AM 8/14/2002, you wrote:
} Does anybody knows the max operating room temp for SEM? } } Thanks, } Pavel
Our custom evaporation fixtures can be found on our web site as David pointed out. The direct address is http://www.laddresearch.com/General_Catalog/Chapter_11/chapter_11.html
I am sure ours could be used in your system with the proper feed through. Please contact me directly if you are interested.
Thanks,
John Arnott Ladd Research 83 Holly Court Williston, VT 05495
On-line Catalog: http://www.laddresearch.com
tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US) fax: 1-802-660-8859 e-mail: sales-at-laddresearch.com ----- Original Message ----- } From: "David Rothbard" {rothbardD-at-netscape.net} To: {Microscopy-at-sparc5.microscopy.com} Sent: Tuesday, August 13, 2002 9:37 PM
Hello Everyone, There has been an increasing number of enquiries regarding lighting systems for fluorescence emission viewing. Our lab is using a number of different systems from BLS, a Hungarian company with many years of experience in the manufacture of biological lab equipment. If you would like to visit the web site, this is the address; http://www.bls-ltd.com/ Regards to all, Stephen Black
Technical Manager Nagylab Rm. 881 Samuel Lunenfeld Research Institute Mount Sinai Hospital 600 University Ave. Toronto, M5G 1X5 Canada
Below is the result of your feedback form. It was submitted by (billiams-at-virgilio.it) on Wednesday, August 14, 2002 at 14:39:45 ---------------------------------------------------------------------------
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Soon after the M&M conference, I wish to give a detailed summary on the question of liquid He transferring kids.
1. It seems that continuous filling of liquid-He is impossible right now, since: } There are two main disadvantages with continuous filling, one is the helium } loss along a long transfer tube, the second is vibration and goniometer } movement problems. Because of the double walled design of transfer tubes, } they are quite stiff and during transfer there is a lot of turbulence at the } exhaust which will destroy any resolution (comments by Bobert Morrison).
2. Another good and detailed suggestion was given by Ron Doole:
} We do not loose vacuum when filling but we do support the transfer tube to } prevent too much strain on the holder. } The initial fill takes about 45 minutes. It is best for 2 people to set up } and remove the He transfer tube, although it is possible for a single } person to do it it just seems safer with two. } Depending on temperature the hold time is generally about 50 mins to 3 } hours (50 mins at 7K or 8K, 2 hours at 15K, 2.5 hours at 21K etc.).
} Subsequent transfers take about 30 minutes. } As we tend to use the holder infrequently and book a week of He stage work } when we want it we will always repump the vacuum jackets on the transfer } dewar and holder before each session.
} The most diffucult thing is to determine when the dewar is full and on the } first trial we shot most of a 50l dewar of He through the holder as we } could not tell the difference between the He gas plume and the He liquid } plume on the exhaust line. With experience we can now fill with about 5L } of He. } It is important to use the gas to cool the holder down to a reasonable } level (50K) to avoid loosing too much liquid.
3. Some points concering whether the holder is filled enough (provided by Robert Morrison): } It looks as if the holder is not being filled enough, as on test here the } hold time was over 1 hour at base temp. Is the dewar well pumped? In } principle when He is filled it should cryopump well so a good rotary pump } vacuum should be sufficient. Does the pressure relief valve blow off during } use showing a high heat load?
I greatly appreciate these kind suggestions and comments. And I wish the person who has also interest in the problem can share their experiences. Still, the question is open for more discussion.
With Best Regards,
Jinsong Wu Department of Physics and Astronomy Arizona State University Tempe, AZ 85287-1504
I am currently in the market for CCD camera for a TEM. I have obtained all the literature and pricing info regarding the various products, but would like some user opinions on performance and quality of service, (especially service.)
I would greatly appreciate any insight anyone may have to offer.
Sincerely,
Cavin Mooers, EM Facility Manager Vitreous State Laboratory The Catholic University of America Hannan Hall Washington, DC 20064 (202) 319-5346phone (202) 319-4469fax
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (fkriss-at-chem.udel.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday, August 14, 2002 at 14:03:17 ---------------------------------------------------------------------------
Email: fkriss-at-chem.udel.edu Name: Frank Kriss
Organization: Univ. of Delaware
Education: Undergraduate College
Location: Newark, DE, USA
Question: I am searching stains for amino acids, lysine, and NH3+ groups in TEM work. Does anyone know a good reference for this type of work?
Pressurex force indicating sensor film is now available in a new trial-size package. Immediately address your tactile surface stress issues by trying the Pressurex force indicating film sample kit for only $149.
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To order: Tel: 973.560.9092, Fax: 973.884.1699, Email: bebner-at-sensorprod.com
Bill Ebner Sensor Products Inc. USA www.sensorprod.com
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Pressurex force indicating sensor film is now available in a new trial-size package. Immediately address your tactile surface stress issues by trying the Pressurex force indicating film sample kit for only $149.
Pressurex sensor film is a thin, flexible sheet (physically similar in appearance to a magazine page) of microencapsulated mylar that instantaneously and permanently changes color in proportion to the amount of force applied upon it. Like Litmus paper, the resultant color changes are quantifiable and can be compared to a calibration table to determine actual PSI values. Applications include examination of gasketed surfaces, bolted joints and clamps, nip impressions, heat seals, lamination presses, composite layups, electronic packaging and more.
The sample kit consists of a one foot sheet of each of Pressurex's six PSI ranges, from 2 PSI to 19,000 PSI, an instruction manual and color calibration tables.
To order: Tel: 973.560.9092, Fax: 973.884.1699, Email: bebner-at-sensorprod.com
Bill Ebner Sensor Products Inc. USA www.sensorprod.com
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Pressurex force indicating sensor film is now available in a new trial-size package. Immediately address your tactile surface stress issues by trying the Pressurex force indicating film sample kit for only $149.
Pressurex sensor film is a thin, flexible sheet (physically similar in appearance to a magazine page) of microencapsulated mylar that instantaneously and permanently changes color in proportion to the amount of force applied upon it. Like Litmus paper, the resultant color changes are quantifiable and can be compared to a calibration table to determine actual PSI values. Applications include examination of gasketed surfaces, bolted joints and clamps, nip impressions, heat seals, lamination presses, composite layups, electronic packaging and more.
The sample kit consists of a one foot sheet of each of Pressurex's six PSI ranges, from 2 PSI to 19,000 PSI, an instruction manual and color calibration tables.
To order: Tel: 973.560.9092, Fax: 973.884.1699, Email: bebner-at-sensorprod.com
Bill Ebner Sensor Products Inc. USA www.sensorprod.com
} From time to time we will send you information about valuable new services, products and special offers from Sensor Products and our preferred business partners that we feel will be of interest to you.
If you do not want to receive these emails please click on the unsubscribe link below.
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I would think that it's the max. operating temperature of the microscopist that you need to concern yourself with. Your chiller should be able to keep the temp. stable for all inhabitable conditions. If it is too hot for the electronics, the microscopist will have been deceased long before an electronic failure.
Peter Tomic
-----Original Message----- } From: ATC SEM Laboratory [mailto:atcsem-at-earthlink.net] Sent: Wednesday, August 14, 2002 11:31 AM To: Microscopy-at-sparc5.microscopy.com
Does anybody knows the max operating room temp for SEM?
You have to decide first: are you going to use optical coupling camera or direct fiber-optic coupling? First type is faster and good for nearly video speed (real time imaging); second type is slower, but has better sensitivity and image quality (no distortions from optics). Second: do you want bottom or top (35 mm port) mount camera? Bottom mount camera is essential for high resolution work, but limited by small field. Top mount camera has bigger field and good for routine work. Third: retractable or not. If retractable - you may use film as well, if non-retractable bottom-mount - forget the film. Most modern cameras are retractable now. When I shop for my camera I find that this market is relatively small, so you don't expect fast service. I mean: they simply don't have enough people in most cases for quick response. And last: check software - you may find very good camera coming with terrible, user unfriendly soft... My advise: always ask for demo (sorry, camera guys) before final decision; took your sample and make your own pictures, compare images from different cameras side by side, you would be surprised to see how different they could be. You may contact me off line if need details. Sergey
At 05:07 PM 8/14/02, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
On Wed, 14 Aug 2002 14:27:44 -0400 Ron L'Herault {lherault-at-bu.edu} wrote: } } Our scope starts to behave badly above 29 deg. C or so. } } Ron L
Our users start to behave badly above 25 deg C.
Stu -------------------------------------------- Stuart Kearns Electron Microbeam Laboratories Department of Earth Sciences University of Bristol Queens Road Bristol BS8 1RJ UK tel: (0)117 954 5435 fax: (0)117 925 3385 e-mail: Stuart.Kearns-at-bristol.ac.uk http://eugf.gly.bris.ac.uk --------------------------------------------
We hit 38deg C last summer. Users complained of feeling sleepy. The service engineer said the electronics should not go above 40 deg. C. If it was 38 on the desk the electonics would be hotter.
Dave
On Thu, 15 Aug 2002 08:47:49 +0100 Stuart Kearns {Stuart.Kearns-at-bristol.ac.uk} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } On Wed, 14 Aug 2002 14:27:44 -0400 Ron L'Herault {lherault-at-bu.edu} } wrote: } } } } Our scope starts to behave badly above 29 deg. C or so. } } } } Ron L } } Our users start to behave badly above 25 deg C. } } Stu } -------------------------------------------- } Stuart Kearns } Electron Microbeam Laboratories } Department of Earth Sciences } University of Bristol } Queens Road } Bristol BS8 1RJ } UK } tel: (0)117 954 5435 } fax: (0)117 925 3385 } e-mail: Stuart.Kearns-at-bristol.ac.uk } http://eugf.gly.bris.ac.uk } -------------------------------------------- } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
A SEM is less critical than a TEM in respect to temperature.
A chiller is used to cool down parts of the microscope acting as an heatsink.
Every mechanical part is changing, as slightly as you want, dimensions with temperature.
Usually, all mechanical measuring instruments (and parts) are tested at the temperature of 20 degree Celsius (I do not know the Imperial standard for such a measurement).
Since this temperature is not exactly very comfortable for the human been, the best thing is to get the microscope in a room with about 24 - 25 degree Celsius, but the important thing is that this temperature is not changing very much and very fast.
Try to imagine the trouble to take a high magnification picture while the stage is warming up or cooling down changing its dimension of 500 nanometres in one hour, and on your screen 1 centimetre represents 50 nanometres.
The mechanical parts of the column too behave the same, but as stated at the beginning, a SEM is less sensitive than a TEM.
Marco Arienti
www.electronrescue.com
----- Original Message ----- } From: "ATC SEM Laboratory" {atcsem-at-earthlink.net} To: {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, August 14, 2002 5:31 PM
Cavin,
Sergey makes a lot of good points, but one point needs correction:
Bottom-mount cameras are usually attached BELOW the film chamber and thus do NOT impede the use of film, retractable or not. The only time you need a retractable bottom-mount camera is if you have other instrments attached at the bottom port and need to use both.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu] Sent: Wednesday, August 14, 2002 10:00 PM To: Cavin Mooers; Microscopy-at-sparc5.microscopy.com
Cavin
You have to decide first: are you going to use optical coupling camera or direct fiber-optic coupling? First type is faster and good for nearly video speed (real time imaging); second type is slower, but has better sensitivity and image quality (no distortions from optics). Second: do you want bottom or top (35 mm port) mount camera? Bottom mount camera is essential for high resolution work, but limited by small field. Top mount camera has bigger field and good for routine work. Third: retractable or not. If retractable - you may use film as well, if non-retractable bottom-mount - forget the film. Most modern cameras are retractable now. When I shop for my camera I find that this market is relatively small, so you don't expect fast service. I mean: they simply don't have enough people in most cases for quick response. And last: check software - you may find very good camera coming with terrible, user unfriendly soft... My advise: always ask for demo (sorry, camera guys) before final decision; took your sample and make your own pictures, compare images from different cameras side by side, you would be surprised to see how different they could be. You may contact me off line if need details. Sergey
At 05:07 PM 8/14/02, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
I've noticed several requests on the ListServer re: antibodies. I refer all of you to Linscott's Directory of Immunological and Biological Reagents (http://www.linscott'sdirectory.com or http://www.linscott'sdirectory.co.uk. It is an excellent source of monoclonal and polyclonal antibodies, etc. Plus, it will direct you to the suppliers of those antibodies, etc.
Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Laboratories of Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
That depends on a number of variables. If you rely on the dimensional certifiable calibration of the SEM, you may have limitations, depending on the circuit design. But the primary consideration is generally the combination of temperature, humidity and if water cooling or EDS is used.
High humidity is a limitation if an EDS system is in use, as the cryogenically cooled EDS 'snout' in the sample chamber will condense water vapor whenever the chamber is vented in a high humidity environment. This will give you problems in pump down times.
If any water cooling is used in the electronics or vacuum systems, the cooling lines can condense water that would be harmful to the systems. Trust me, I have charged thousands to repair damage caused by small amounts of water introduced to electronic circuits.
I have assumed (perhaps wrongly) that by high temperatures you mean a lack of control of the ambient environment. In other words, an area that doesn't have air conditioning. If the instrument is operated in a high temperature environment that doesn't also have a high humidity, you are only accelerating the normal lifetime of the electronics. When you bring a high humidity into the environment you are introducing a variety of effects that can bring the whole system down in ways you can't imagine.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174
On Wednesday, August 14, 2002 8:31 AM, ATC SEM Laboratory [SMTP:atcsem-at-earthlink.net] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Does anybody knows the max operating room temp for SEM? } } Thanks, } Pavel } } } }
While on the subject. Does anyone have any comments on humidity in and around a SEM?
Robert Fowler Quality Assurance Technician (Failure Analysis) TDK Components USA, Inc. Multilayer Ceramic Capacitor Division 1 TDK Boulevard Peachtree City GA 30269-2051 Telephone: (770) 631-0410 Ext.315 Fax: (770) 487-1460 email: rfowler-at-tdktca.com www.tdk.com
THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL INFORMATION.
If you are not the intended recipient, be advised that any use, dissemination, distribution or duplication of this transmission is strictly prohibited. If you received this transmission in error, please notify the sender immediately by electronic reply to this transmission or by phone (847-803-6100). Thank you.
At 07:02 AM 8/15/02, you wrote: } Cavin, } } Sergey makes a lot of good points, but one point needs correction: } } Bottom-mount cameras are usually attached BELOW the film chamber and thus do } NOT impede the use of film, retractable or not. The only time you need a } retractable bottom-mount camera is if you have other instrments attached at } the bottom port and need to use both. } } mike } } } Michael Bode, Ph.D. } Soft Imaging System Corp. } 12596 West Bayaud Avenue } Suite 300 } Lakewood, CO 80228 } =================================== } phone: (888) FIND SIS } (303) 234-9270 } fax: (303) 234-9271 } email: mailto:info-at-soft-imaging.com } web: http://www.soft-imaging.com } =================================== } } } } -----Original Message----- } From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu] } Sent: Wednesday, August 14, 2002 10:00 PM } To: Cavin Mooers; Microscopy-at-sparc5.microscopy.com } Subject: Re: TEM CCD Imaging - Seeking User Insights to Various } Manufacturers } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Could I please get everyone who handles clinical cases to pass on how they deal with the issue of CAP- proficiency testing. I am unaware of any "official testing" of clinical EM labs. Does anyone know of one? How do you address this issue in your Procedure Manual? Thanks for all your help.
Lauren E. Chesnut Technical Director Electron Microscopy Lab University of Texas Health- Science Center at San Antonio Dept. of Pathology (210)567-4052 chesnutl-at-uthscsa.edu
In regards to the web address of the linscott's directory make sure you type linscotts: www.linscottsdirectory.com
Karen L. Bentley, M.S.(previously Jensen) Associate Scientist & Project Manager Electron Microscope Research Core University of Rochester Medical Center Rochester, NY 14642 585-275-1954
Debby,
I've noticed several requests on the ListServer re: antibodies. I refer all of you to Linscott's Directory of Immunological and Biological Reagents (http://www.linscott'sdirectory.com or http://www.linscott'sdirectory.co.uk. It is an excellent source of monoclonal and polyclonal antibodies, etc. Plus, it will direct you to the suppliers of those antibodies, etc.
Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Laboratories of Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
Here is a interesting question. What is the average time to set up a complete electron Microscope Laboratory. This include portioning, workbenches, electrical connections, Gas connections as well as TEM, SEM, CLSM for both the biological as well as Material science field.
Furthermore what is a realistic timeframe to have it all commissioned, running smoothly and producing publications as well as getting the new academic community accustomed to the abilities?
Do any of the users of the Nikon Eclipse have a recommended cleaning procedure for the optics. I have a spot of dust or other material viewed through the eyepiece and camera This has been present after one month of purchase and I used it to "trademark" pictures taken from this scope. But it is now an annoyance. Another problem I had was my light source became darker over time. The problem was not in filament degradation but in the first optic which spreads or concentrates the beam. This optic had a haze of some sort. Easily cleaned off but now has me concerned about the high humidity present in the lab. Has anyone experienced this?
Robert Fowler Quality Assurance Technician (Failure Analysis) TDK Components USA, Inc. Multilayer Ceramic Capacitor Division 1 TDK Boulevard Peachtree City GA 30269-2051 Telephone: (770) 631-0410 Ext.315 Fax: (770) 487-1460 email: rfowler-at-tdktca.com www.tdk.com
THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL INFORMATION.
If you are not the intended recipient, be advised that any use, dissemination, distribution or duplication of this transmission is strictly prohibited. If you received this transmission in error, please notify the sender immediately by electronic reply to this transmission or by phone (847-803-6100). Thank you.
We found with our TE200 that a collection filter near the Hg arc lamp popped out because the epoxy holding it in got brittle. The images had dark areas. The precise location of the problem took us a long time to figure out because of the way it fell inside the lamp housing opening.
People who use too much oil cause it to drip onto the filter blocks. These dark spots are easily cleaned.
Basically, to find dirt, just go through the light path looking at every surface. Because of infinity correction, anything between the back of the objective and the camera can be particulary problematic.
Your question encompasses a great deal of ground so I will simply tell you my experience.
We moved into a second building in 1998 that was abandoned for years prior to us taking possession. That is, it was literally open to the sky with holes in the ceiling and had to be renovated from the ground up, everything but the concrete foundation which fortunately was nice and thick, 16 inches, and very stable. The process of restoring this building took a year but it was in deplorable condition. I will assume you are starting with a building that is inhabitable, clean and has climate/humidity control.
We had to do a site survey after the building was in order for electromagnetic fields and floor stability with a seismic instrument. This took just a few days. Fortunately there were no issues. This is not always the case if you are near large horsepower electric motors, electric closets, elevators or heavy vehicular traffic. You may need to mitigate these conditions if adverse and that may be time consuming if you need structural modifications [permits required in most places] to the building like a "floating" floor, structural members or electromagnetic isolation/shielding or getting someone to move a soda machine away from the SEM column. The soda machine people never like this and generally don't care about your SEM nor your scientific pursuits. Fortunately, in our case, none of these issues arose.
If you have a chiller for the SEM you won't have to deal with a chilled water supply. Chilled house water supply could be time consuming, plumbing, permits, etc. Gasses such as CDA [clean dry air], nitrogen and vacuum, if not already in the lab., may take several weeks to install depending on where the feeds are if there are any at all.
I purchased a Hitachi S4500/2 FESEM, dropped it into place and with the help of Hitachi's field service folks was able to use the instrument in 5 days. In short, after the utilities were in and the SEM delivered, we were functional with few problems that were facility related. Vacuum systems are another issue entirely.
The time to get an academic community accustomed to anything is essentially a function of the community and not the instrumentation or facility. I'd like to hear back from you in a year or so on that one.
I hope this is of some help and I would imagine others on this listserver have additional experiences to share.
Good luck.
Peter Tomic Anadigics, Inc.
-----Original Message----- } From: Coetzee, Mr S. H Physics Science [mailto:COETZEES-at-mopipi.ub.bw] Sent: Friday, August 16, 2002 11:30 AM To: Microscopy-at-sparc5.microscopy.com
Dear all
Here is a interesting question. What is the average time to set up a complete electron Microscope Laboratory. This include portioning, workbenches, electrical connections, Gas connections as well as TEM, SEM, CLSM for both the biological as well as Material science field.
Furthermore what is a realistic timeframe to have it all commissioned, running smoothly and producing publications as well as getting the new academic community accustomed to the abilities?
*This message was transferred with a trial version of CommuniGate(tm) Pro* Dear Ian, I am interested in HRTEM images simulation. I do not know if I can help you. I would like to know what kind of computer program you use after the generation of the supercell in order to simulate the HRTEM images. I have used EMS program, but first I have to generate a supercell file (where I indicate all the information present in a normal crystal file, which can be create with EMS itself) by myself: this file should have a well defined extension you have to respect in order to apply the sub-program to reproduce the effect of the microscope. Also with Cerius2 program you have to write a well defined extension before simulating the effect of the microscope. Could you please give me some more indication about the program you have used to create the supercell? It should be very useful for me because it is often necessary to use a supercell to simulate the real specimens of which the scientific world is interested. Thanks Marilena
Ian MacLaren wrote:
} *This message was transferred with a trial version of CommuniGate(tm) Pro* } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear all, } I have used the program CrystalKit for building supercells for the } simulation of HREM images of GBs and planar defects. Is there any } competition to this program, or is really the only package worth considering } for this purpose. } } Thanks } } Ian MacLaren } N.B. New address } TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung } Petersenstr. 23, 64287 Darmstadt, Germany } Tel: +49 6151 162894 } ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/
Micrograph Competition :: The Art of Microscopy A micrograph competition will be held at ICEM-15 to highlight the art of science. The competition will be open to all forms of microscopic imaging. Winners will be selected on artistic merit and general audience appeal. The image may be a single image or a montage of several images. The rules of entry are: Any individual may submit a (maximum) of two entries. Entries must have an overall dimension of 27.5cm x 35.0cm, and can be mounted vertically or horizontally. Entries must be affixed to a stiff lightweight support, such as 10mm foam board, and may be mounted so as to have borders. Each entry must have a separate text sheet 23cm x 30cm with a title centred at the top of the page, followed by two single carriage returns, and a 200 word, maximum, description of the image. Times Roman 14pt font is recommended. The entrants name, address and image title must be on the back of the micrograph mount. Entries must be brought to the congress and mounted on the competition display board by noon on Monday 2 September and must be removed between 09h00 and 12h00 on Friday morning. Micrographs remaining on the display board after this will be donated to local schools. Prizes for the winning micrographs will be awarded at the banquet on Thursday evening. So please don't forget to pack those pictures before you step on to the plane.
Luc Harmsen Marketing ICEM15 www.ICEM15.com 1-6 September 2002 Durban, South Africa
Morning All, I am looking for some last-minute, off-list, advice from any of you who have Technai 12's up and running. Ours is due in 3 weeks.
Thanks,
Fred
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center, Room SS024 West Chester University of Pennsylvania South Church Street & Rosedale Ave. West Chester, PA, 19383 Phone & FAX: 610-738-0437 eMail: fmonson-at-wcupa.edu http://darwin.wcupa.edu/casi/
I apologize for re-posting this message, I have had trouble with my e-mail. How do clinical labs deal with the College of American Pathologist inspection question about proficiency testing of the lab?
Lauren E. Chesnut Technical Director Electron Microscopy Lab University of Texas Health Science Center at San Antonio 7703 Floyd Curl Drive San Antonio, TX. 78229-3900 Dept. of Pathology (210)567-4052
I hope, this isn't a crossposting: I recently analyzed different silica modifications in meteorites. I got one spectra I could not identify. It isn't qtz, trd, crs, cs, stishovite, melanophlogite or moganite. From the spectra we can say that the modification should have low symmetry and low specific density. Therefore we think it could be keatite. This mineral often occurs in hydrothermal experiments between 300 and 600°C. The exact stability field is not known. I am know looking for a keatite sample, to take a raman spectra and compare it to the one I obtained. And there is my question: Can anyone lend me a sample of keatite? It is not important, whether it is within a thin section or a grain. The sample can be quite small, as we use microraman. The sample will not be destroyed and does not need any preparation and will certainly send back pretty soon.
It would obviously be better, if someone has a raman spectra of keatite, which I could have. I can also send my spectra to anyone who is interested and can maybe say something about it. Or - even better - does anyone has a raman spectra data base of different silica modifications? Who knows, what we found maybe isn't keatite at all ...
best regards,
Dominik Hezel
_____________ Dominik Hezel Institut für Mineralogie und Geochemie der Universität zu Köln Zülpicherstr. 49b 50674 Köln Tel.: +49 (0221) 470 32 28 e-mail: d.hezel-at-uni-koeln.de
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
I'm sure this is not very OSH-friendly, but when we do Au-Pd evaporation (we never do carbon-coating any more), the tungsten filament in the evaporator glows white-hot, and I suspect that it's not at all good for you. We have a plexiglass "implosion protection shield" - sort of a big cylinder with a closed upper end that fits over the evaporator's bell jar. This is supposed to contain flying shards of glass should the bell jar ever implode. I sincerely hope it never happens.... Anyway, as far as protecting your eyes from the bright light, we've simply placed a few strips of masking tape around the implosion protection shield at the level(s) that people would be looking through, and it does a good job of shielding the glare. You can still see what's going on during the evaporation process. You should probably avoid sticking anything to the bell jar itself - anything that may induce the slightest extra stress in a bell jar (especially an old one) under high vacuum is not good. No doubt some kind of dark safety glasses would be better, as a safety measure, I'm sure, but an actual welder's mask may be a bit of overkill. Of course, it would be good implosion protection....
Frank Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada
----- Original Message ----- } From: "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Monday, August 19, 2002 2:05 PM
Hello,
We have acquired a vintage but healthy Hitachi Vacuum Evaporator, model HUS-4. Since it requires all manual operation of valves etc., I would really like to find a copy of the operator's manual if one exists.
Please reply directly to me
Karen Rethoret Biology Department York University 4700 Keele St. Toronto, Ontario M3J 1P3
What you need to do, since CAP doesn¹t send out a specific QC test for EM, is to design a program yourself. The method is unspecified. They will accept a genuine effort on your part to examine your product and ensure that it is first class.
What we do is to send out micrographs, along with clinical histories, to a competent electron microscopist at other institutions. (CAP likes to see interaction between labs, rather than just asking a pathologist at your own institution to look at your work). This needs to be done every 6 months. We have 3 different folks who review our work (2-5 cases, one to 5 micrographs/case), each person once every 18 months (only one reviewer every 6 months). They send back a report that basically says that the work is acceptable for the purpose that it is designed. One is a pathologist, and the other two are PhD lab directors. Look at the CAP questions and design a questionnaire for the reviewer that asks whether certain standards are met. Make it as easy as possible for the reviewer; i.e., don¹t ask for a written report; just make a checklist of things like quality and correct diagnosis. Also, include a place for signature and title of the reviewer. Include a stamped, self-addressed envelope for return of the micrographs and questionnaire. Send it to another lab director or physician with some familiarity with the process.
Hope this helps.
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Arc welding glass works very well once the arc is struck and you can watch the carbon rods decrease very nicely. You can just buy the glass for the helmet and it works just fine. One of the EM supply houses (EMS or SPI?) has them in their catalog.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center P. O. Box 11472 (letters) Guys Run Rd. (packages) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8515 (fax)
-----Original Message----- } From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu] Sent: Monday, August 19, 2002 1:06 PM To: Microscopy-at-sparc5.microscopy.com
Dear Listers,
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
Hi: Our refrigerator/freezer broke while I was on vacation. The chemicals and samples felt warm to the touch upon return. They were without cold temps for 2-14 days. Can they be salvaged or should I just start over. A list of chem below:
Osmium tetroxide (100% crystal & 4% aqueous in ampules) Glutaraldehyde and paraformaldehyde (10-70% ampules) Goat Serum Various anti-bodies LR Whites resin
Thanks for any input, Charlie Murphy
Charles Murphy USDA, ARS, Electron Microscopy Unit Bldg. 177B, BARC East Beltsville, MD, 20705 (301) 504-8046 (301) 504-8923 fax murphyc-at-ba.ars.usda.gov
A welder's helmet would certainly do the job. Not only does it reduce the intensity but it removes lots of UV.
There are some new high-tech units that use a controllable LCD do adjust the amount of opacity. I presume that it too reduces UV. Welding supply houses carry these accessories.
gary
At 10:05 AM 8/19/2002, you wrote:
} Dear Listers, } } For many years, I've used nothing but self-discipline to avoid direct eye } contact with the white-hot electrodes resulting during a carbon evaporation } run. However, times have changed and now I am responsible for the safety of } student users. } } Does anyone know whether an arc-welder's face-shield would protects the eyes } from this type of intense light? My impression is that the face-shield } mainly protects the welder from emitted sparks, and I don't know whether the } light emission is similar to that from carbon evaporation. } } If not, what eye protection are others using out there in List-land? } } Thanks as always for your help. } } Ann } } ################### } Ann Hein Lehman } Assistant Director, EM Facility } Trinity College - LSC314 } 300 Summit Street } Hartford CT 06106 } v. 860-297-4289 } f. 860-297-2538 } e. ann.lehman-at-trincoll.edu } w. http://www.trincoll.edu/~alehman/ } Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm } } } -----Original Message----- } } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] } Sent: Monday, August 19, 2002 9:22 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Administrivia: June 2002 Archives are now On-Line } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I am posting the following message from Richard Cartun in case any of you knew David Knibbs and didn't hear about his death and in case any of you might be able to help Dr. Cartun out in finding someone who could carry on as lab director. If anyone would like more information, please contact Dr. Cartun at the address and phone below or at his e-mail address: Rcartun-at-harthosp.org
Regards, Karen Pawlowski, Ph.D. Research Scientist UT Dallas
Richard Cartun wrote: It is with great sadness that I announce the untimely death of my friend and colleague, David R. Knibbs, Ph.D. He collapsed and died after an evening run (one of his joys in life) on Monday, August 5th. David (49 years old) was our Director of Electron Microscopy and worked with me very closely here at Hartford Hospital. Like me, David completed his Ph.D. studies in Pathobiology at the University of Connecticut. We will miss him enormously.
Now, we must begin our search for a replacement (I'm not sure that is the right word since I don't believe David will ever be replaced) and I must turn to the Histonet for help. We feel it is imperative to maintain our quality EM service here at our hospital, but realize that finding a qualified director who will also be called upon to do all the technical work (David was a "one man show") will be most difficult. Please contact me if you have any suggestions or recommendations. Thank you.
I know this sound a bit rough, but here is our horror story. When I arrived here at the University of Botswana all our chemical was not in a fridge for 6 months. This include temperatures above 36 degrees! Sofar our LR white is still working fine after being refrigerated again as well as the Osmium and Glute.
At least some chemicals appear to be a bit more robust that suggested.
Hope this helps
-----Original Message----- } From: Charles Murphy [mailto:murphyc-at-ba.ars.usda.gov] Sent: Monday, August 19, 2002 11:09 PM To: Microscopy-at-sparc5.microscopy.com
Hi: Our refrigerator/freezer broke while I was on vacation. The chemicals and samples felt warm to the touch upon return. They were without cold temps for 2-14 days. Can they be salvaged or should I just start over. A list of chem below:
Osmium tetroxide (100% crystal & 4% aqueous in ampules) Glutaraldehyde and paraformaldehyde (10-70% ampules) Goat Serum Various anti-bodies LR Whites resin
Thanks for any input, Charlie Murphy
Charles Murphy USDA, ARS, Electron Microscopy Unit Bldg. 177B, BARC East Beltsville, MD, 20705 (301) 504-8046 (301) 504-8923 fax murphyc-at-ba.ars.usda.gov
Dear all, Has anyone done Lorentz microscopy in a Philips CM20? If so, have you any good practical tips as to how I should adjust the microscope for optimal performance in this purpose? How should I adjust the lenses to minimise the field at the sample, for instance?
Dear Microscopists, we have an old Reichert-Jung Cryostat Freezing Microtome Model 975C. The microtome still works perfect but the blade is getting dull. Does anyone know whom to contact for a re-sharpening of the blades or evenentually for a blade replacement? Thanks very much. Yours, Frank
_____________________________________
Frank Bungartz Lichen Herbarium Department of Plant Biology Arizona State University (ASU) PO Box 87 1601 Tempe, AZ 85287 - 1601 USA
For in house use we use goggles, which we also sell along with our evaporators. You can check them out at http://www.laddresearch.com/General_Catalog/Chapter_7/Safety_Aids/Personal_P rotection/personal_protection.html
JD Arnott
Ladd Research 83 Holly Court Williston, VT 05495
On-line Catalog: http://www.laddresearch.com
tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US) fax: 1-802-660-8859 e-mail: sales-at-laddresearch.com ----- Original Message ----- } From: "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Monday, August 19, 2002 1:05 PM
Dear Listers,
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
Our new detector has been damaged. I will try to install our old detector in JMS-840 SEM for a temporary use, before I can get a new detector. This old detector (Link Pentafet plus detector) was used in the same SEM around one and half years ago. Parameters of the old detector are given in the following:
Model: 6276 Det. Area: 10.0 mmxmm Window: 0.008 Resol.: 137 ev at 5.99 ekv Serial: 1080-3929 Att. No.: 32cc32088
The manual shows the EXCHANGE DETECTOR procedure. But this procedure does not indicate details on filling liquid nitrogen. If anybody of you has experience with installation of this type of detectors, I would like to have your opinions and thoughts. This detector has not used for one and half years. Also I have some questions on installation of the detector as follows.
1) When liquid nitrogen will be filled? I think that first the detector assembly will be fixed in the SEM, and then liquid nitrogen will be filled until the chamber vacuum is ready. 2) I don't know if the old detector can be thermally cycled. What procedure (warm up & cool down?) will be performed during filling liquid nitrogen? Or an easy way is just to fill liquid nitrogen, then and wait for the next calibration after four hours ?
Any suggestions and recommendation on installation of this old detector will be greatly appreciated. Thank you for your help.
Yuquan Ding Dept of Mechanical Engineering University of Waterloo Waterloo, ON Canada N2L 3G1 Tel: 519-888-4567 ext 3766
Dear Ann, For years I have used a pair of welder's goggles with my vacuum evaporator. Since there is no danger of sparks there is no need for full head protection and they are a little easier to wear than the full welder's helmet, so the students are more likely to put them on. You are correct in assuming that the UV from the arc is dangerous to the eyes and may contrubute to cataract formation. At 01:05 PM 08/19/2002 -0400, you wrote: } } } Dear Listers, } } For many years, I've used nothing but self-discipline to avoid direct eye } contact with the white-hot electrodes resulting during a carbon evaporation } run. However, times have changed and now I am responsible for the safety of } student users. } } Does anyone know whether an arc-welder's face-shield would protects the eyes } from this type of intense light? My impression is that the face-shield } mainly protects the welder from emitted sparks, and I don't know whether the } light emission is similar to that from carbon evaporation. } } If not, what eye protection are others using out there in List-land? } } Thanks as always for your help. } } Ann } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
I am a business man in Dallas Texas up until recently I have been a very successful one. please let me start off by telling you this is not a plea, I am not begging I am simply offering a great business opportunity to anyone who has the foresight and guts to take a chance here is a little of where I am coming from.
I am forty years old and I have worked hard all of my life I have a beautiful wife and three fantastic kids I have everything I need in life including having accepted Jesus Christ as my lord and savior. my children are all happy and healthy and my marriage is fantastic as a matter of fact we are celebrating our twentieth anniversary soon.
due to some tragedies in my life that I will explain if we talk I have taken a financial loss that I have not been able to get back up from. my back is against the wall I am paying over two thousand dollars a week to people I should not have borrowed from but I do pay it every week. I am able when I am not starting from this deep in the hole to make great amounts of money in short periods of time so here is my proposition.
I need forty thousand dollars to pay off all of the friends who have helped me all of the sharks I borrowed from and to live on until I get going again.
in return I will pay this loan back in installments of 2000.00 a month for forty months that is a one hundred percent return on your investment. this is not a scam I will meet you in person I have excellent character and business references that will tell you I am a man of my word. I am not asking for bank accounts or wires all transactions will be done in person if you can.
like I said, if you have foresight and guts, I promise you will not regret it please only serious inquires only this a legitimate business offer. I will only do this with one person. so if you are at all interested please email me at:
equitablems-at-eurosport.com
I know this sounds crazy but believe me I have tried everything else there is and until I get out of the hole I just aint gonna make it. thanking you in advance, LC
god bless everyone this gets to whether you are able to help or not.
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Arc welding glass works very well once the arc is struck and you can watch the carbon rods decrease very nicely. You can just buy the glass for the helmet and it works just fine. One of the EM supply houses (EMS or SPI?) has them in their catalog.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center P. O. Box 11472 (letters) Guys Run Rd. (packages) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8515 (fax)
-----Original Message----- } From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu] Sent: Monday, August 19, 2002 1:06 PM To: Microscopy-at-sparc5.microscopy.com
Dear Listers,
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dear Listers,
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi: Our refrigerator/freezer broke while I was on vacation. The chemicals and samples felt warm to the touch upon return. They were without cold temps for 2-14 days. Can they be salvaged or should I just start over. A list of chem below:
Osmium tetroxide (100% crystal & 4% aqueous in ampules) Glutaraldehyde and paraformaldehyde (10-70% ampules) Goat Serum Various anti-bodies LR Whites resin
Thanks for any input, Charlie Murphy
Charles Murphy USDA, ARS, Electron Microscopy Unit Bldg. 177B, BARC East Beltsville, MD, 20705 (301) 504-8046 (301) 504-8923 fax murphyc-at-ba.ars.usda.gov
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Hello,
We have acquired a vintage but healthy Hitachi Vacuum Evaporator, model HUS-4. Since it requires all manual operation of valves etc., I would really like to find a copy of the operator's manual if one exists.
Please reply directly to me
Karen Rethoret Biology Department York University 4700 Keele St. Toronto, Ontario M3J 1P3
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A welder's helmet would certainly do the job. Not only does it reduce the intensity but it removes lots of UV.
There are some new high-tech units that use a controllable LCD do adjust the amount of opacity. I presume that it too reduces UV. Welding supply houses carry these accessories.
gary
At 10:05 AM 8/19/2002, you wrote:
} Dear Listers, } } For many years, I've used nothing but self-discipline to avoid direct eye } contact with the white-hot electrodes resulting during a carbon evaporation } run. However, times have changed and now I am responsible for the safety of } student users. } } Does anyone know whether an arc-welder's face-shield would protects the eyes } from this type of intense light? My impression is that the face-shield } mainly protects the welder from emitted sparks, and I don't know whether the } light emission is similar to that from carbon evaporation. } } If not, what eye protection are others using out there in List-land? } } Thanks as always for your help. } } Ann } } ################### } Ann Hein Lehman } Assistant Director, EM Facility } Trinity College - LSC314 } 300 Summit Street } Hartford CT 06106 } v. 860-297-4289 } f. 860-297-2538 } e. ann.lehman-at-trincoll.edu } w. http://www.trincoll.edu/~alehman/ } Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm } } } -----Original Message----- } } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] } Sent: Monday, August 19, 2002 9:22 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Administrivia: June 2002 Archives are now On-Line } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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Hello everyone,
I am posting the following message from Richard Cartun in case any of you knew David Knibbs and didn't hear about his death and in case any of you might be able to help Dr. Cartun out in finding someone who could carry on as lab director. If anyone would like more information, please contact Dr. Cartun at the address and phone below or at his e-mail address: Rcartun-at-harthosp.org
Regards, Karen Pawlowski, Ph.D. Research Scientist UT Dallas
Richard Cartun wrote: It is with great sadness that I announce the untimely death of my friend and colleague, David R. Knibbs, Ph.D. He collapsed and died after an evening run (one of his joys in life) on Monday, August 5th. David (49 years old) was our Director of Electron Microscopy and worked with me very closely here at Hartford Hospital. Like me, David completed his Ph.D. studies in Pathobiology at the University of Connecticut. We will miss him enormously.
Now, we must begin our search for a replacement (I'm not sure that is the right word since I don't believe David will ever be replaced) and I must turn to the Histonet for help. We feel it is imperative to maintain our quality EM service here at our hospital, but realize that finding a qualified director who will also be called upon to do all the technical work (David was a "one man show") will be most difficult. Please contact me if you have any suggestions or recommendations. Thank you.
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Ann -
I'm sure this is not very OSH-friendly, but when we do Au-Pd evaporation (we never do carbon-coating any more), the tungsten filament in the evaporator glows white-hot, and I suspect that it's not at all good for you. We have a plexiglass "implosion protection shield" - sort of a big cylinder with a closed upper end that fits over the evaporator's bell jar. This is supposed to contain flying shards of glass should the bell jar ever implode. I sincerely hope it never happens.... Anyway, as far as protecting your eyes from the bright light, we've simply placed a few strips of masking tape around the implosion protection shield at the level(s) that people would be looking through, and it does a good job of shielding the glare. You can still see what's going on during the evaporation process. You should probably avoid sticking anything to the bell jar itself - anything that may induce the slightest extra stress in a bell jar (especially an old one) under high vacuum is not good. No doubt some kind of dark safety glasses would be better, as a safety measure, I'm sure, but an actual welder's mask may be a bit of overkill. Of course, it would be good implosion protection....
Frank Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada
----- Original Message ----- } From: "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Monday, August 19, 2002 2:05 PM
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Dear Charles
I know this sound a bit rough, but here is our horror story. When I arrived here at the University of Botswana all our chemical was not in a fridge for 6 months. This include temperatures above 36 degrees! Sofar our LR white is still working fine after being refrigerated again as well as the Osmium and Glute.
At least some chemicals appear to be a bit more robust that suggested.
Hope this helps
-----Original Message----- } From: Charles Murphy [mailto:murphyc-at-ba.ars.usda.gov] Sent: Monday, August 19, 2002 11:09 PM To: Microscopy-at-sparc5.microscopy.com
Hi: Our refrigerator/freezer broke while I was on vacation. The chemicals and samples felt warm to the touch upon return. They were without cold temps for 2-14 days. Can they be salvaged or should I just start over. A list of chem below:
Osmium tetroxide (100% crystal & 4% aqueous in ampules) Glutaraldehyde and paraformaldehyde (10-70% ampules) Goat Serum Various anti-bodies LR Whites resin
Thanks for any input, Charlie Murphy
Charles Murphy USDA, ARS, Electron Microscopy Unit Bldg. 177B, BARC East Beltsville, MD, 20705 (301) 504-8046 (301) 504-8923 fax murphyc-at-ba.ars.usda.gov
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Morning All, I am looking for some last-minute, off-list, advice from any of you who have Technai 12's up and running. Ours is due in 3 weeks.
Thanks,
Fred
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center, Room SS024 West Chester University of Pennsylvania South Church Street & Rosedale Ave. West Chester, PA, 19383 Phone & FAX: 610-738-0437 eMail: fmonson-at-wcupa.edu http://darwin.wcupa.edu/casi/
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Dear Microscopists, we have an old Reichert-Jung Cryostat Freezing Microtome Model 975C. The microtome still works perfect but the blade is getting dull. Does anyone know whom to contact for a re-sharpening of the blades or evenentually for a blade replacement? Thanks very much. Yours, Frank
_____________________________________
Frank Bungartz Lichen Herbarium Department of Plant Biology Arizona State University (ASU) PO Box 87 1601 Tempe, AZ 85287 - 1601 USA
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Micrograph Competition :: The Art of Microscopy A micrograph competition will be held at ICEM-15 to highlight the art of science. The competition will be open to all forms of microscopic imaging. Winners will be selected on artistic merit and general audience appeal. The image may be a single image or a montage of several images. The rules of entry are: Any individual may submit a (maximum) of two entries. Entries must have an overall dimension of 27.5cm x 35.0cm, and can be mounted vertically or horizontally. Entries must be affixed to a stiff lightweight support, such as 10mm foam board, and may be mounted so as to have borders. Each entry must have a separate text sheet 23cm x 30cm with a title centred at the top of the page, followed by two single carriage returns, and a 200 word, maximum, description of the image. Times Roman 14pt font is recommended. The entrants name, address and image title must be on the back of the micrograph mount. Entries must be brought to the congress and mounted on the competition display board by noon on Monday 2 September and must be removed between 09h00 and 12h00 on Friday morning. Micrographs remaining on the display board after this will be donated to local schools. Prizes for the winning micrographs will be awarded at the banquet on Thursday evening. So please don't forget to pack those pictures before you step on to the plane.
Luc Harmsen Marketing ICEM15 www.ICEM15.com 1-6 September 2002 Durban, South Africa
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Dear all,
I hope, this isn't a crossposting: I recently analyzed different silica modifications in meteorites. I got one spectra I could not identify. It isn't qtz, trd, crs, cs, stishovite, melanophlogite or moganite. From the spectra we can say that the modification should have low symmetry and low specific density. Therefore we think it could be keatite. This mineral often occurs in hydrothermal experiments between 300 and 600°C. The exact stability field is not known. I am know looking for a keatite sample, to take a raman spectra and compare it to the one I obtained. And there is my question: Can anyone lend me a sample of keatite? It is not important, whether it is within a thin section or a grain. The sample can be quite small, as we use microraman. The sample will not be destroyed and does not need any preparation and will certainly send back pretty soon.
It would obviously be better, if someone has a raman spectra of keatite, which I could have. I can also send my spectra to anyone who is interested and can maybe say something about it. Or - even better - does anyone has a raman spectra data base of different silica modifications? Who knows, what we found maybe isn't keatite at all ...
best regards,
Dominik Hezel
_____________ Dominik Hezel Institut für Mineralogie und Geochemie der Universität zu Köln Zülpicherstr. 49b 50674 Köln Tel.: +49 (0221) 470 32 28 e-mail: d.hezel-at-uni-koeln.de
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I apologize for re-posting this message, I have had trouble with my e-mail. How do clinical labs deal with the College of American Pathologist inspection question about proficiency testing of the lab?
Lauren E. Chesnut Technical Director Electron Microscopy Lab University of Texas Health Science Center at San Antonio 7703 Floyd Curl Drive San Antonio, TX. 78229-3900 Dept. of Pathology (210)567-4052
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*This message was transferred with a trial version of CommuniGate(tm) Pro* Dear Ian, I am interested in HRTEM images simulation. I do not know if I can help you. I would like to know what kind of computer program you use after the generation of the supercell in order to simulate the HRTEM images. I have used EMS program, but first I have to generate a supercell file (where I indicate all the information present in a normal crystal file, which can be create with EMS itself) by myself: this file should have a well defined extension you have to respect in order to apply the sub-program to reproduce the effect of the microscope. Also with Cerius2 program you have to write a well defined extension before simulating the effect of the microscope. Could you please give me some more indication about the program you have used to create the supercell? It should be very useful for me because it is often necessary to use a supercell to simulate the real specimens of which the scientific world is interested. Thanks Marilena
Ian MacLaren wrote:
} *This message was transferred with a trial version of CommuniGate(tm) Pro* } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear all, } I have used the program CrystalKit for building supercells for the } simulation of HREM images of GBs and planar defects. Is there any } competition to this program, or is really the only package worth considering } for this purpose. } } Thanks } } Ian MacLaren } N.B. New address } TU-Darmstadt, FB Materialwissenschaft, FG Strukturforschung } Petersenstr. 23, 64287 Darmstadt, Germany } Tel: +49 6151 162894 } ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/
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What you need to do, since CAP doesn¹t send out a specific QC test for EM, is to design a program yourself. The method is unspecified. They will accept a genuine effort on your part to examine your product and ensure that it is first class.
What we do is to send out micrographs, along with clinical histories, to a competent electron microscopist at other institutions. (CAP likes to see interaction between labs, rather than just asking a pathologist at your own institution to look at your work). This needs to be done every 6 months. We have 3 different folks who review our work (2-5 cases, one to 5 micrographs/case), each person once every 18 months (only one reviewer every 6 months). They send back a report that basically says that the work is acceptable for the purpose that it is designed. One is a pathologist, and the other two are PhD lab directors. Look at the CAP questions and design a questionnaire for the reviewer that asks whether certain standards are met. Make it as easy as possible for the reviewer; i.e., don¹t ask for a written report; just make a checklist of things like quality and correct diagnosis. Also, include a place for signature and title of the reviewer. Include a stamped, self-addressed envelope for return of the micrographs and questionnaire. Send it to another lab director or physician with some familiarity with the process.
Hope this helps.
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
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Ann,
For in house use we use goggles, which we also sell along with our evaporators. You can check them out at http://www.laddresearch.com/General_Catalog/Chapter_7/Safety_Aids/Personal_P rotection/personal_protection.html
JD Arnott
Ladd Research 83 Holly Court Williston, VT 05495
On-line Catalog: http://www.laddresearch.com
tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US) fax: 1-802-660-8859 e-mail: sales-at-laddresearch.com ----- Original Message ----- } From: "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Monday, August 19, 2002 1:05 PM
Dear Listers,
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dear all, Has anyone done Lorentz microscopy in a Philips CM20? If so, have you any good practical tips as to how I should adjust the microscope for optimal performance in this purpose? How should I adjust the lenses to minimise the field at the sample, for instance?
And I, I of the old school, use my genuine, official, AO-Polaroid, WWII, flying-ace, flight glasses. Whenever the C-sun gets brighter, I just turn the knob and cross my polarizers. And, they were free!!!!
For the students, there is nothing so flashy. They get good, pre-tested, welders goggles, but there is nothing like crossed and crosser polarizers.
Unfortunately, I've lost the optics from my submarine periscope and I can't use my goggles from around the corner of the room. I am now thinking of the old wooden box with two mirrors trick.
As for self-discipline, I lost that sometime during my wedding night, but I have been searching for it ever since. And, like so many [old(?)] men after 35+ years of marriage, I hurry home each night to buss the wife and smile my secret smiles - still clueless about it all. And while the Sry gene finally gives some meaning to the existence of the Y chromosome, we who have been thus pre-determined feel short-changed without the ability to choose, that having been taken from us by an arbitrary sequence of nucleotides for which we neither asked nor were given the power or right to exorcise. Yet, still, I humbly count my blessings. I could have been born a Medaca, with two Y's, and still been twice oppositely determined, in an estrogenized aquarium, to carry XX plumbing. Would that have been an example of one unfulfilled expectation, or would that count as two?
"And now", she says, "he feels content, having new company in his clueless abyss where no one knows what makes a man - or a woman". And, if your baby Medacas are born with "incorrect" chromosomes, you may merely have to spike the water to set things right!
FC Monson - THE STUDENTS ARE BACK!!!! Is there not a song with words like, "pre-meds every the morning, and pre-meds every night, ..."?
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center, Room SS024 West Chester University of Pennsylvania South Church Street & Rosedale Ave. West Chester, PA, 19383 Phone & FAX: 610-738-0437 eMail: fmonson-at-wcupa.edu http://darwin.wcupa.edu/casi/
-----Original Message----- } From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu] Sent: Monday, August 19, 2002 1:06 PM To: Microscopy-at-sparc5.microscopy.com
Dear Listers,
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
This is clearly (what we call in the States) "Too much information."
Earl
Original Message: ----------------- } From: Monson, Frederick C. fmonson-at-wcupa.edu
And I, I of the old school, use my genuine, official, AO-Polaroid, WWII, flying-ace, flight glasses. Whenever the C-sun gets brighter, I just turn the knob and cross my polarizers. And, they were free!!!!
For the students, there is nothing so flashy. They get good, pre-tested, welders goggles, but there is nothing like crossed and crosser polarizers.
Unfortunately, I've lost the optics from my submarine periscope and I can't use my goggles from around the corner of the room. I am now thinking of the old wooden box with two mirrors trick.
As for self-discipline, I lost that sometime during my wedding night, but I have been searching for it ever since. And, like so many [old(?)] men after 35+ years of marriage, I hurry home each night to buss the wife and smile my secret smiles - still clueless about it all. And while the Sry gene finally gives some meaning to the existence of the Y chromosome, we who have been thus pre-determined feel short-changed without the ability to choose, that having been taken from us by an arbitrary sequence of nucleotides for which we neither asked nor were given the power or right to exorcise. Yet, still, I humbly count my blessings. I could have been born a Medaca, with two Y's, and still been twice oppositely determined, in an estrogenized aquarium, to carry XX plumbing. Would that have been an example of one unfulfilled expectation, or would that count as two?
"And now", she says, "he feels content, having new company in his clueless abyss where no one knows what makes a man - or a woman". And, if your baby Medacas are born with "incorrect" chromosomes, you may merely have to spike the water to set things right!
FC Monson - THE STUDENTS ARE BACK!!!! Is there not a song with words like, "pre-meds every the morning, and pre-meds every night, ..."?
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center, Room SS024 West Chester University of Pennsylvania South Church Street & Rosedale Ave. West Chester, PA, 19383 Phone & FAX: 610-738-0437 eMail: fmonson-at-wcupa.edu http://darwin.wcupa.edu/casi/
-----Original Message----- } From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu] Sent: Monday, August 19, 2002 1:06 PM To: Microscopy-at-sparc5.microscopy.com
Dear Listers,
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
-------------------------------------------------------------------- mail2web - Check your email from the web at http://mail2web.com/ .
Your recent post has appalled me, and I suspect many others; Earl Weltmer let you down kindly and diplomatically. I routinely delete your posts which clog up my mailbox. Sorry, but you need to hear this.
Yours
Jeremy Sanderson
__________________________________________________ Do You Yahoo!? Everything you'll ever need on one web page from News and Sport to Email and Music Charts http://uk.my.yahoo.com
We are having some difficulty with getting good thick sections from feline retinal tissue. The sections look somewhat uneven in thickness (wavy), and in the nerve fiber layer and at the level of the inner limiting membrane there is vacuolization. There are also tears in some areas. Our standard processing procedures use 2% gluteraldehyde/2% paraformaldehyde in 0.1M cacodylate buffer, 1% osmium post-fix, 1% uranyl acetate en bloc stain, ethanol dehydration, and Spurr's/EPON resin. We are currently trying 2.5% gluteraldehyde in cacodylate (at 0.1 and 0.17M), as well as combining microwave fixation with our standard procedures.
Our current procedures---microwave and otherwise---work very well with mouse retinal tissue, but cats are being contrary. If anyone has any tried and true techniques, I'd be very grateful to hear about them. It could save us tons of time and frustration as we develop a new set of protocols.
Thanks very much.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
----- Original Message ----- } From: "Earl Weltmer" {earlw-at-sbcglobal.net} To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com} ; "Monson, Frederick C." {fmonson-at-wcupa.edu} ; {microscopy-at-sparc5.microscopy.com} Sent: Wednesday, August 21, 2002 10:46 AM
Dear Mr. Ding, As your old detector has been sitting without liquid nitrogen for several months, it must be considered to have been thermally cycled, that is, warmed up, at least once. For the health of the window, the best sequence is: 1. install the detector on the microscope, which is vented. 2. Fill the dewar with liquid nitrogen. This will increase the vacuum in the dewar, so there will be no chance of pressure on the wrong side of the window. 3. Pump down the SEM. 4. After four hours of liquid nitrogen fill, connect the cables from the analyser, then turn on the bias voltage to the detector. Wait at least one-half hour for the system to stabilize before taking spectra. 5. Calibrate the detector. You may have to find the coarse and fine pots to match the old detector to the analyser. The time your detector was not cold may have caused some degradation in the resolution or peak tailing, but maybe not too much. At 11:50 AM 08/20/2002 -0400, you wrote: } } Dear Listers: } } Our new detector has been damaged. I will try to install our } old detector in JMS-840 SEM for a temporary use, before I can get a new } detector. This old detector (Link Pentafet plus detector) was used in the } same SEM around one and half years ago. Parameters of the old detector are } given in the following: } } Model: 6276 } Det. Area: 10.0 mmxmm } Window: 0.008 } Resol.: 137 ev at 5.99 ekv } Serial: 1080-3929 } Att. No.: 32cc32088 } } The manual shows the "EXCHANGE DETECTOR" procedure. But this procedure } does not indicate details on filling liquid nitrogen. If anybody of you has } experience with installation of this type of detectors, I would like to have } your opinions and thoughts. This detector has not used for one and half } years. Also I have some questions on installation of the detector as } follows. } } 1) When liquid nitrogen will be filled? I think that first the detector } assembly will be fixed in the SEM, and then liquid nitrogen will be filled } until the chamber vacuum is ready. } 2) I don't know if the old detector can be thermally cycled. What procedure } (warm up & cool down?) will be performed during filling liquid nitrogen? Or } an easy way is just to fill liquid nitrogen, then and wait for the } next calibration after four hours ? } } Any suggestions and recommendation on installation of this old detector will } be greatly appreciated. Thank you for your help. } } Yuquan Ding } Dept of Mechanical Engineering } University of Waterloo } Waterloo, ON Canada N2L 3G1 } Tel: 519-888-4567 ext 3766 } Regards and good luck! Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm looking to add a digital camera to and I have gotten rather confused by all the choices out there. I could use some advice regarding what are the important things to look for.
Recently I have been making more use of this microscope and have been dissatisfied with the old Polaroid camera attachment used to take pictures of the samples. I would like to junk that and replace it with a PC based color digital camera. I still don't foresee heavy use for this microscope, but my needs (hair images) are more demanding than the previous rather casual use we made of this guy.
I have seen a number of choices up at the M&M show and am collecting literature and quotes. But I wonder about whether attaching a higher end consumer camera (I have literature for a Kodak DC290, for example) will be good enough or if I should spend more money on a more specialized camera, like a Spot Insight that a local vendor has demonstrated for me (seemed nice enough). What is important and what not?
And where does one get a C mount since we don't have one and the cameras don't necessarily seem to come with one.
Many thanks as always.
Richard Shalvoy Arch Chemicals, Inc. 350 Knotter Drive Cheshire, CT 06410 (203) 271-4394 rbshalvoy-at-archchemicals.com
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It has been a long time but, if I remember correctly (which I would not guarantee), I think that you can only do Lorentz microscopy in a standard CM in the low mag mode, when the objective is "off" (it actually has a small current for aberation correction). Unfortunately, this limits the maximum magnifaction to ~x3,000.
In the main mag modes, the objective lens field is on and much too strong, causing the specimens to completely saturate (and sometimes pulling them out of the holder). You will also require a 'small' objective aperture to generate Lorentz contrast - however, in the LM mode, this means a medium aperture by comparision with those normally used in the main mag ranges - 50 micron?
Theer are a few (3?) CMs around with special 'Lorentz' objectives, allowing imaging of magnetic domains at much higher magnifications (x30,000).
I must declare an interest, currently being an employee of JEOL UK. -- Larry Stoter
Hello, I've got a clay in a resin I would like to section, but ordinary water won't work. As soon as the clay hits water, it disperses and comes out of the resin. I used to make light microscope thin sections using kerosene for clays like these instead of water. Is there another fluid I could use for sectioning the samples other than water? Kerosene just jumps to the block and wets the whole face.
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ Gordon Ante Vrdoljak Electron Microscope Lab ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall gvrdolja-at-nature.berkeley.edu UC Berkeley phone (510) 642-2085 Berkeley CA 94720-3330 fax (510) 643-6207 cell (510) 290-6793
I will send you information about our cameras by mail, but I wanted to address a couple of the points you are raising here:
1) You mention the Kodak DC290. That camera is actually discontinued.
2) If you purchase a camera like the Kodak DC290, make sure that you can get uncompressed images into your computer. Most cameras compress the images using a JPG algorithm, which introduces artifacts. Depending on what you do, these artifacts may be severe enough to prevent further working with the images.
3) Live viewing with this type of camera may be restricted to the LCD monitor on the camera (or viewfinder), which is normally too small to do anything.
4) Some of the cheaper cameras may use a cheap lens, potentially adding to distortion and reducing resolution.
5) You need a special holder for these cameras, as they usually don't adhere to any standards.
6) I have never seen any specifications about the sensitivity and dynamic range of the consumer cameras. I suppose it's not that great.
7) C-mount: Most scientific cameras come with a C-mount, which is really nothing more thrilling than a certain thread and some geometric considerations on the camera side. If you want to connect this to your microscope, you need a c-mount adapter, which you can get from the microscope manufacturer. C-mount adapters come in different flavors, with different lenses. Depending on the chip-size of the camera you need a specific c-mount if you want to keep the field of view the same through binoculars and camera.
8) Resolution: There is a wide variety of scientific cameras with different resolutions. In general you need the higher resolution for a larger field of view. For example, an image taken on a 1300x1024 camera at 1000x is probably not worse than one taken with a 200x1500 camera, as the resolution is determined by the N.A. of the lens. On the other hand, at a lower magnification, the larger chip can show more details for the same field of view.
I hope I have not confused you more with this information. Give me a call or drop me an email if you have any questions.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Shalvoy, Richard B **CHES [mailto:RBShalvoy-at-archchemicals.com] Sent: Wednesday, August 21, 2002 12:14 PM To: Microscopy-at-sparc5.microscopy.com
I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm looking to add a digital camera to and I have gotten rather confused by all the choices out there. I could use some advice regarding what are the important things to look for.
Recently I have been making more use of this microscope and have been dissatisfied with the old Polaroid camera attachment used to take pictures of the samples. I would like to junk that and replace it with a PC based color digital camera. I still don't foresee heavy use for this microscope, but my needs (hair images) are more demanding than the previous rather casual use we made of this guy.
I have seen a number of choices up at the M&M show and am collecting literature and quotes. But I wonder about whether attaching a higher end consumer camera (I have literature for a Kodak DC290, for example) will be good enough or if I should spend more money on a more specialized camera, like a Spot Insight that a local vendor has demonstrated for me (seemed nice enough). What is important and what not?
And where does one get a C mount since we don't have one and the cameras don't necessarily seem to come with one.
Many thanks as always.
Richard Shalvoy Arch Chemicals, Inc. 350 Knotter Drive Cheshire, CT 06410 (203) 271-4394 rbshalvoy-at-archchemicals.com
Well, I've always been fascinated by what human society would be like if we behaved like coral trout. Would one gender treat the other with infinite understanding, or would we follow a pattern like school yard bullying and boot camp hazing? Opens up a huge range of possibilities in between, anyway.
Dont know how you find the time Fred, but I always enjoy your posts.
Sally Stowe
} } } "earlw-at-sbcglobal.net" {earlw-at-sbcglobal.net} 08/21/02 07:00PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
This is clearly (what we call in the States) "Too much information."
Earl
Original Message: ----------------- } From: Monson, Frederick C. fmonson-at-wcupa.edu
And I, I of the old school, use my genuine, official, AO-Polaroid, WWII, flying-ace, flight glasses. Whenever the C-sun gets brighter, I just turn the knob and cross my polarizers. And, they were free!!!!
For the students, there is nothing so flashy. They get good, pre-tested, welders goggles, but there is nothing like crossed and crosser polarizers.
Unfortunately, I've lost the optics from my submarine periscope and I can't use my goggles from around the corner of the room. I am now thinking of the old wooden box with two mirrors trick.
As for self-discipline, I lost that sometime during my wedding night, but I have been searching for it ever since. And, like so many [old(?)] men after 35+ years of marriage, I hurry home each night to buss the wife and smile my secret smiles - still clueless about it all. And while the Sry gene finally gives some meaning to the existence of the Y chromosome, we who have been thus pre-determined feel short-changed without the ability to choose, that having been taken from us by an arbitrary sequence of nucleotides for which we neither asked nor were given the power or right to exorcise. Yet, still, I humbly count my blessings. I could have been born a Medaca, with two Y's, and still been twice oppositely determined, in an estrogenized aquarium, to carry XX plumbing. Would that have been an example of one unfulfilled expectation, or would that count as two?
"And now", she says, "he feels content, having new company in his clueless abyss where no one knows what makes a man - or a woman". And, if your baby Medacas are born with "incorrect" chromosomes, you may merely have to spike the water to set things right!
FC Monson - THE STUDENTS ARE BACK!!!! Is there not a song with words like, "pre-meds every the morning, and pre-meds every night, ..."?
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center, Room SS024 West Chester University of Pennsylvania South Church Street & Rosedale Ave. West Chester, PA, 19383 Phone & FAX: 610-738-0437 eMail: fmonson-at-wcupa.edu http://darwin.wcupa.edu/casi/
-----Original Message----- } From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu] Sent: Monday, August 19, 2002 1:06 PM To: Microscopy-at-sparc5.microscopy.com
Dear Listers,
For many years, I've used nothing but self-discipline to avoid direct eye contact with the white-hot electrodes resulting during a carbon evaporation run. However, times have changed and now I am responsible for the safety of student users.
Does anyone know whether an arc-welder's face-shield would protects the eyes from this type of intense light? My impression is that the face-shield mainly protects the welder from emitted sparks, and I don't know whether the light emission is similar to that from carbon evaporation.
If not, what eye protection are others using out there in List-land?
Thanks as always for your help.
Ann
################### Ann Hein Lehman Assistant Director, EM Facility Trinity College - LSC314 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman/ Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
-----Original Message----- } From: J-H Lignot [mailto:J-H.Lignot-at-c-strasbourg.fr] Sent: Monday, August 19, 2002 9:22 AM To: Microscopy-at-sparc5.microscopy.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Colleagues...
The June 2002 archives of the Microscopy Listserver are now on-line.
at http://www.msa.microscopy.com/MicroscopyListserver
Cheers...
Nestor Your Friendly Neighborhood SysOp
Dr Jean-Hervé Lignot, Université Louis Pasteur / CNRS CEPE 23 rue Becquerel, 67087 Strasbourg, Cedex 2, France Fax: 00 33 0388106906 Tel: 00 33 0388106938
-------------------------------------------------------------------- mail2web - Check your email from the web at http://mail2web.com/ .
Randy: It has been a long time since I have done cat retina, but there were are few papers along the way. The only real difference I see in protocols (I don't think the glut/para vs glut is a problem--I generally use 2%pf + 1% glut, but have used straight glut as well) is that I have stayed with straight Epon (usually EMBED 812 from EM Sciences, but others have worked equally well) with little or no tearing or wrinkling. As for the vacuolization, are these eyes perfused or immersion fixed? My experience (from rats to cats to dogs to primates) is that vacuolization generally occurs during removal of the eye (due to too much force being applied or too much pulling) or the enucleation process. Another variable can be the trimming of the samples for EM--i.e. using a crushing cut rather than a slicing cut. Don't know if any of these are your problems. Just things I have had to work out over the years.
Roger Moretz -- Where the world is only slightly less weird than it actually is. } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Listers, } } We are having some difficulty with getting good thick sections from feline } retinal tissue. The sections look somewhat uneven in thickness (wavy), and in } the nerve fiber layer and at the level of the inner limiting membrane there is } vacuolization. There are also tears in some areas. Our standard processing } procedures use 2% gluteraldehyde/2% paraformaldehyde in 0.1M cacodylate buffer, } 1% osmium post-fix, 1% uranyl acetate en bloc stain, ethanol dehydration, and } Spurr's/EPON resin. We are currently trying 2.5% gluteraldehyde in cacodylate } (at 0.1 and 0.17M), as well as combining microwave fixation with our standard } procedures. } } Our current procedures---microwave and otherwise---work very well with mouse } retinal tissue, but cats are being contrary. If anyone has any tried and true } techniques, I'd be very grateful to hear about them. It could save us tons of } time and frustration as we develop a new set of protocols. } } Thanks very much. } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-5414 } Email: tindallr-at-missouri.edu } Web: http://www.biotech.missouri.edu/emc/ } } }
I think that Fred deserves another open opportunity to post.
This was not information overload. Rather, it was a dearth of information, as I see it. I think that he was caught at a bad time. S.... happens. Am I wrong or am I wright?
Only he knows.
gary g.
At 04:25 PM 8/21/2002, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I've embedded some cat retina, using my "basic" method, with fine results. The user fixes (I like cats too much!) - sometimes glut (2.5% in 0.1M cacodylate pH 7.4), sometimes Karnovsky's, immersion or infused. Then 2% Os 2 hrs, UAc 1hr, EtOH 10-15 mins each, 2x acetone 15 min each, Epon/Araldite embed. I know with human retina that too much vitreous really messes up embedding - could that be a problem with cats?
on 8/19/02 1:05 PM, Lehman, Ann at Ann.Lehman-at-trincoll.edu wrote: } } For many years, I've used nothing but self-discipline to avoid direct eye } contact with the white-hot electrodes resulting during a carbon evaporation } run. However, times have changed and now I am responsible for the safety of } student users. } } Does anyone know whether an arc-welder's face-shield would protects the eyes } from this type of intense light? My impression is that the face-shield } mainly protects the welder from emitted sparks, and I don't know whether the } light emission is similar to that from carbon evaporation. } } If not, what eye protection are others using out there in List-land? } } Thanks as always for your help. } Dear Ann, Even the most trouble-prone student is very unlikely to have an eye in direct contact with a white-hot electrode ;-). Welding goggles or a face shield will protect against the intense light. The light from the carbon rods and that from a welding arc are sufficiently similar that the welders' eye protection will work well. The welding arc does have more UV than the carbon rods--especially as the latter are inside a glass bell jar and plastic implosion shield. Now all you need to do is to be sure that the students actually wear the gear and lower the shield when they start up the current. Good luck. Yours, Bill Tivol
Colleagues, is anybody aware of a method to purify active HRP (horseradish peroxidase)from native tissue (e.g. horseradish). High purity of isolate would not be necessary. Thanks for any suggestion, Peter Heimann ********************************** peter.heimann-at-uni-bielefeld.de Dr. Peter Heimann Developmental Biology & Molecular Pathology; W7-107 University of Bielefeld D 33501 Bielefeld / Germany phone: xx49(0)521-106-5628 / 5627 FAX : " " - 5654 WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie/ WEB-Site: http://www.uni-bielefeld.de/SFB549 ***********************************
We have a digital camera attached to our light microscope with a coupler purchased from Thales-Optem in Fairport, NY. Their phone number is 716-223-2370 x 178 and the person to talk with about your questions is Patrick Harrington. I found him to be extremely helpful with my questions, and he even suggested a Canadian supplier as well, for a C mount for our dissecting scope.
Hope this helps,
Susan
Susan Carbyn Electron Microscopy Technician Atlantic Food and Horticulture Research Centre Agriculture and Agri-Food Canada 32 Main Street, Kentville, N.S. B4N 1J5 Canada
Phone (902) 679-5535 Fax (902) 679-2311
E-Mail: carbyns-at-em.agr.ca
} } } "Shalvoy, Richard B **CHES" {RBShalvoy-at-archchemicals.com} 08/21/02 03:14PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm looking to add a digital camera to and I have gotten rather confused by all the choices out there. I could use some advice regarding what are the important things to look for.
Recently I have been making more use of this microscope and have been dissatisfied with the old Polaroid camera attachment used to take pictures of the samples. I would like to junk that and replace it with a PC based color digital camera. I still don't foresee heavy use for this microscope, but my needs (hair images) are more demanding than the previous rather casual use we made of this guy.
I have seen a number of choices up at the M&M show and am collecting literature and quotes. But I wonder about whether attaching a higher end consumer camera (I have literature for a Kodak DC290, for example) will be good enough or if I should spend more money on a more specialized camera, like a Spot Insight that a local vendor has demonstrated for me (seemed nice enough). What is important and what not?
And where does one get a C mount since we don't have one and the cameras don't necessarily seem to come with one.
Many thanks as always.
Richard Shalvoy Arch Chemicals, Inc. 350 Knotter Drive Cheshire, CT 06410 (203) 271-4394 rbshalvoy-at-archchemicals.com
I'd be happy to solicit loans and grants from the folks on the listserver. Albeit I'm in New Jersey and not Nigeria but I can do a pretty good fake Nigerian accent, especially if money is involved.
I think folks need to lighten up and I doubt whether Fred intended to be insulting to any species. As a married man with kids I know that humor is the best remedy at times for things that ail us. I can start a listserver just on my own domestic frustra.
Peter
-----Original Message----- } From: Earl Weltmer [mailto:earlw-at-sbcglobal.net] Sent: Wednesday, August 21, 2002 1:56 PM To: Microscopy-at-sparc5.microscopy.com
----- Original Message ----- } From: "Earl Weltmer" {earlw-at-sbcglobal.net} To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com} ; "Monson, Frederick C." {fmonson-at-wcupa.edu} ; {microscopy-at-sparc5.microscopy.com} Sent: Wednesday, August 21, 2002 10:46 AM
http://www.unwords.com/sniglets/frustra.shtml
----- Original Message ----- } From: Peter Tomic {PTomic-at-anadigics.com}
Area code for this 716 area is now 585
-----Original Message----- } From: Susan Carbyn [mailto:CarbynS-at-agr.gc.ca] Sent: Thursday, August 22, 2002 8:52 AM To: Microscopy-at-sparc5.microscopy.com
Hi Richard,
We have a digital camera attached to our light microscope with a coupler purchased from Thales-Optem in Fairport, NY. Their phone number is 716-223-2370 x 178 and the person to talk with about your questions is Patrick Harrington. I found him to be extremely helpful with my questions, and he even suggested a Canadian supplier as well, for a C mount for our dissecting scope.
Hope this helps,
Susan
Susan Carbyn Electron Microscopy Technician Atlantic Food and Horticulture Research Centre Agriculture and Agri-Food Canada 32 Main Street, Kentville, N.S. B4N 1J5 Canada
Phone (902) 679-5535 Fax (902) 679-2311
E-Mail: carbyns-at-em.agr.ca
} } } "Shalvoy, Richard B **CHES" {RBShalvoy-at-archchemicals.com} 08/21/02 03:14PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm looking to add a digital camera to and I have gotten rather confused by all the choices out there. I could use some advice regarding what are the important things to look for.
Recently I have been making more use of this microscope and have been dissatisfied with the old Polaroid camera attachment used to take pictures of the samples. I would like to junk that and replace it with a PC based color digital camera. I still don't foresee heavy use for this microscope, but my needs (hair images) are more demanding than the previous rather casual use we made of this guy.
I have seen a number of choices up at the M&M show and am collecting literature and quotes. But I wonder about whether attaching a higher end consumer camera (I have literature for a Kodak DC290, for example) will be good enough or if I should spend more money on a more specialized camera, like a Spot Insight that a local vendor has demonstrated for me (seemed nice enough). What is important and what not?
And where does one get a C mount since we don't have one and the cameras don't necessarily seem to come with one.
Many thanks as always.
Richard Shalvoy Arch Chemicals, Inc. 350 Knotter Drive Cheshire, CT 06410 (203) 271-4394 rbshalvoy-at-archchemicals.com
I don't know how, but a previous was sent in rich rather than plain text. My apology.
The following URL is for a company that claims to produce new and have parts for Technicon processors. I have never contacted this company, but the URL has come up several times in searches on the net. For those who are interested, here it is.
http://www.datacut.com/gg/products.htm
Hope this helps some,
FCM
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center, Room SS024 West Chester University of Pennsylvania South Church Street & Rosedale Ave. West Chester, PA, 19383 Phone & FAX: 610-738-0437 eMail: fmonson-at-wcupa.edu http://darwin.wcupa.edu/casi/
Please specify which email that was sent in rich instead of plain text: the one about your wife or the "Technicon Processor".
Earl Weltmer
----- Original Message ----- } From: "Monson, Frederick C." {fmonson-at-wcupa.edu} To: "List-HistoPath (E-mail)" {histonet-at-pathology.swmed.edu} ; "List-Microscopy (E-mail)" {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, August 22, 2002 11:29 AM
Hello, Fellows,
We are planning to buy the GIF for our new JEOL 2010F, could somebody let us know what is the reasonable cost to buy this system?
Thanks a lot!
James
_________________________________________________________________ Chat with friends online, try MSN Messenger: http://messenger.msn.com
Does anybody know the cost to buy GIF 2000 (Gatan Image Filter) system?
Thanks a lot!
James
_________________________________________________________________ MSN Photos is the easiest way to share and print your photos: http://photos.msn.com/support/worldwide.aspx
If you just let the bell jar get good and dirty, and never clean it, after a while nobody will be able to see anything inside the bell jar, and you won't need goggles or anything.
John Mardinly Intel
-----Original Message----- } From: "MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com [mailto:"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com] Sent: Thursday, August 22, 2002 8:53 AM To: Peter Tomic Cc: 'Earl Weltmer'; Microscopy-at-sparc5.microscopy.com
http://www.unwords.com/sniglets/frustra.shtml
----- Original Message ----- } From: Peter Tomic {PTomic-at-anadigics.com}
I'm not sure that I would agree with this. If the coating was very thick, that might be OK. But we are dealing with both brightness and UV. How thick of a coating is needed to prevent UV damaging eye exposure? Who knows?
I would not risk it. A $75 hood is well worth my eyes for my lifetime. Who suffers during the build up of coating? Not me. Will you?
gary g.
At 06:32 PM 8/22/2002, you wrote:
} If you just let the bell jar get good and dirty, and never clean it, after a } while nobody will be able to see anything inside the bell jar, and you won't } need goggles or anything. } } John Mardinly } Intel } } } } -----Original Message----- } } From: "MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com } [mailto:"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com] } Sent: Thursday, August 22, 2002 8:53 AM } To: Peter Tomic } Cc: 'Earl Weltmer'; Microscopy-at-sparc5.microscopy.com } Subject: Re: RE: Eye Protection and Other Frustra } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Gary, UV is adsorbed by fairly thin plastic and unless the glass belljar is made of quartz no UV will penetrate through it.
JVN
Gary Gaugler wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I'm not sure that I would agree with this. If the coating } was very thick, that might be OK. But we are dealing } with both brightness and UV. How thick of a coating is } needed to prevent UV damaging eye exposure? Who } knows? } } I would not risk it. A $75 hood is well worth my eyes } for my lifetime. Who suffers during the build up of } coating? Not me. Will you? } } gary g. } } } At 06:32 PM 8/22/2002, you wrote: } } } If you just let the bell jar get good and dirty, and never clean it, } } after a } } while nobody will be able to see anything inside the bell jar, and you } } won't } } need goggles or anything. } } } } John Mardinly } } Intel } } } } } } } } -----Original Message----- } } } From: "MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com } } [mailto:"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com] } } Sent: Thursday, August 22, 2002 8:53 AM } } To: Peter Tomic } } Cc: 'Earl Weltmer'; Microscopy-at-sparc5.microscopy.com } } Subject: Re: RE: Eye Protection and Other Frustra } } } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } http://www.unwords.com/sniglets/frustra.shtml } } } } ----- Original Message ----- } } } From: Peter Tomic {PTomic-at-anadigics.com} } } Date: Thursday, August 22, 2002 7:40 am } } Subject: RE: Eye Protection and Other Frustra } } } } } ------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } } AmericaTo Subscribe/Unsubscribe -- Send Email to } } } ListServer-at-MSA.Microscopy.ComOn-Line Help } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html-------- } } } ---------------------------------------------------------------. } } } } } } } } } Earl; } } } } } } I'd be happy to solicit loans and grants from the folks on the } } } listserver.Albeit I'm in New Jersey and not Nigeria but I can do a } } } pretty good fake } } } Nigerian accent, especially if money is involved. } } } } } } I think folks need to lighten up and I doubt whether Fred intended } } } to be } } } insulting to any species. As a married man with kids I know that } } } humor is } } } the best remedy at times for things that ail us. I can start a } } } listserverjust on my own domestic frustra. } } } } } } Peter } } } } } } -----Original Message----- } } } } From: Earl Weltmer [mailto:earlw-at-sbcglobal.net] } } } Sent: Wednesday, August 21, 2002 1:56 PM } } } To: Microscopy-at-sparc5.microscopy.com } } } Subject: Fw: Eye Protection and Other Frustra } } } } } } } } } ------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } } AmericaTo Subscribe/Unsubscribe -- Send Email to } } } ListServer-at-MSA.Microscopy.ComOn-Line Help } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html-------- } } } ---------------------------------------------------------------. } } } } } } } } } } } } ----- Original Message ----- } } } } From: "Earl Weltmer" {earlw-at-sbcglobal.net} } } } To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com} ; "Monson, } } } Frederick C." } } } {fmonson-at-wcupa.edu} ; {microscopy-at-sparc5.microscopy.com} } } } Sent: Wednesday, August 21, 2002 10:46 AM } } } Subject: Re: Eye Protection and Other Frustra } } } } } } } } } } Well, I wasn't appalled but humored which we all need from time } } } to time. } } } } } } } } Which reminds me, it seem that the List has been rather } } } "listless" of } } } late. } } } } } } } } It seem that we don't get the numbers nor the level of info that I } } } normally } } } } receive. } } } } } } } } Is it real or just my perception? } } } } } } } } I was even looking forward to another letter from Nigeria asking } } } for my } } } bank } } } } account number. } } } } By the way, I have received so many "Nigerian Letters" that I } } } have been } } } } forwarding them to each other. Hopefully they can con each other. } } } } } } } } Maybe that "Businessman named Helen from Dallas" can use their help. } } } } } } } } } } } } Yes I do have a life but I look forward to the Listserver info } } } as well. } } } } } } } } Best Regards All, } } } } } } } } } } } } Earl } } } } } } } } ----- Original Message ----- } } } } From: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com} } } } } To: "Monson, Frederick C." {fmonson-at-wcupa.edu} ; } } } } {microscopy-at-sparc5.microscopy.com} } } } } Sent: Wednesday, August 21, 2002 5:30 AM } } } } Subject: Re: Eye Protection and Other Frustra } } } } } } } } } } } } } --------------------------------------------------------------- } } } --------- } } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society } } } of America } } } } } To Subscribe/Unsubscribe -- Send Email to } } } ListServer-at-MSA.Microscopy.Com} } On-Line Help } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } ---- } } } -------------------------------------------------------------------. } } } } } } } } } } } } } } } Dear Mr Monson, } } } } } } } } } } Your recent post has appalled me, and I suspect many } } } } } others; Earl Weltmer let you down kindly and } } } } } diplomatically. I routinely delete your posts which } } } } } clog up my mailbox. Sorry, but you need to hear this. } } } } } } } } } } Yours } } } } } } } } } } Jeremy Sanderson } } } } } } } } } } } } } } } } } } } } __________________________________________________ } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
-- John V Nailon Executive Officer and Operations Manager The Centre for Microscopy and Microanlaysis The University of Queensland St Lucia QLD 4072 Tel: +61-7-33654214 Fax: +61-7-33654422 WWW: http://www.uq.edu.au/nanoworld
Check out http://www.thales-optem.com and their digital camera adapters.
I think that is the route we are going to take as an additional back up for a couple of our microscopes. We have a few Nikon 990 3.34MP digital cameras in the department and for the $325 they ask for the ocular tube adapter is down right affordable.
And BTW - don't you mean 'Light' microscope. Most microscopes electron and visible wave length are "Optical" :D ;) (sorry couldn't help it)
Geoff Williams Microscopy Facility Supervisor
Checkout the new Biology Department Microscopy Facility web page. Version 1 is now On-Line: www.cst.cmich.edu/users/willi1gl/BDMF/BDMF.htm
} -----Original Message----- } From: Shalvoy, Richard B **CHES [mailto:RBShalvoy-at-archchemicals.com] } Sent: Wednesday, August 21, 2002 2:14 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: Adding a digital camera to an optical microscope } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have a lightly used 10 year old Nikon Optiphot-POL microscope that I'm } looking to add a digital camera to and I have gotten rather confused by } all } the choices out there. I could use some advice regarding what are the } important things to look for. } } Recently I have been making more use of this microscope and have been } dissatisfied with the old Polaroid camera attachment used to take pictures } of the samples. I would like to junk that and replace it with a PC based } color digital camera. I still don't foresee heavy use for this } microscope, } but my needs (hair images) are more demanding than the previous rather } casual use we made of this guy. } } I have seen a number of choices up at the M&M show and am collecting } literature and quotes. But I wonder about whether attaching a higher end } consumer camera (I have literature for a Kodak DC290, for example) will be } good enough or if I should spend more money on a more specialized camera, } like a Spot Insight that a local vendor has demonstrated for me (seemed } nice } enough). What is important and what not? } } And where does one get a C mount since we don't have one and the cameras } don't necessarily seem to come with one. } } Many thanks as always. } } Richard Shalvoy } Arch Chemicals, Inc. } 350 Knotter Drive } Cheshire, CT 06410 } (203) 271-4394 } rbshalvoy-at-archchemicals.com }
I have been working with rabbit retina that has been lased at different pulses/pulse durations, looking for damage in the epithelial layer. I have had excellent preservation of the retina (the normal adjacent areas as well as the damaged areas). I recently purchased a microwave to do fixation and processing, and ran a couple of duplicate samples to compare. I also got good results.
Because I haven't found a really inclusive protocol for microwave processing (I couldn't get my blocks to polymerize via the microwave), I resorted to using a conventional oven for that stage.
THE PROTOCOL:
1. The eyes were injected with Karnovsky's Fixative( usually, sometimes with 4% glut) after lasing and left overnight in the cold (this is to maintain the integrity of the structures).
2. The posterior segment of the eye is cut away and then put in fresh 4% Glutaraldehyde in 0.1M Cacodylate Buffer (pH 7.4) and stored in the cold. Note: these segments can remain in the cold indefinitely (i.e. two weeks).
3. The vitreous fluid is teased away gently so as not to disrupt the underlying retinal structures. The lased areas are dissected away from rest of posterior segment using an ophthalmological knife*. Again, to avoid disrupting the structures.
4. The lased segments (-at-1mm square) are put into 0.1M Cacodylate Buffer (pH 7.4) until processing.
Processing:
5. 3 X 15 minutes - 0.1M Cacodylate Buffer 6. Overnight fixation in 2% OsO4 in cacodylate buffer (in the cold) 7. 2 X 15 minutes - 0.1M Cacodylate Buffer 8. Dehydration: 25% ETOH, 50% ETOH (2 X 15 minute changes each); 70% ETOH (2 X 30 minute changes) This is a good stopping point: then I leave them in a 3rd change of 70% overnight in the cold. 9. 2 X 15 minutes - 95% ETOH 10. 4 X 15 minutes - 100% ETOH 11. 2 X 15 minutes - propylene oxide 12. 2:1 propylene oxide:epon (overnight or minimum 2 hours) 13. 1:2 propylene oxide:epon (overnight or minimum 2-3 hours) 14. 100% epon - all day and then embed at end of day. 15. Polymerize in 60 degree oven overnight (18 hours). (I have actually left them for 2 days in the oven and the blocks have been fine. I hesitate to leave them too long; the blocks then get brittle)
I order my embedding components from Tousimis (Rockville, MD): EPON 812 - Cat# 3131 DDSA - Cat# 3123 NMA - Cat# 3143 DMP-30 - Cat# 3103A
I store components under hood at rm. temperature and freeze aliquots of complete epon. I use aliquots for the interim steps (i.e.with propylene oxide) but make up fresh epon for embedding. I make up the epon on a stir plate, stirring after addition of each component. I then put it under vacuum to get rid of excess air bubbles.
I realize everyone's protocol is slightly different (judging from the responses you've received), but as long as you maintain the structure of the retina, you should have no problem with vacuoles or tears in the tissue.
Hope this helps. Now...if you could send me a protocol for microwave processing, I would greatly appreciate it!
Peggy Sherwood Lab Associate, Photopathology Wellman Laboratories of Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
} -----Original Message----- } From: Tindall, Randy D. [SMTP:TindallR-at-missouri.edu] } Sent: Wednesday, August 21, 2002 10:14 AM } To: microscopy-at-sparc5.microscopy.com } Subject: TEM/LM: Problems with cat retinal tissue } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Listers, } } We are having some difficulty with getting good thick sections from feline } retinal tissue. The sections look somewhat uneven in thickness (wavy), and } in the nerve fiber layer and at the level of the inner limiting membrane } there is vacuolization. There are also tears in some areas. Our standard } processing procedures use 2% gluteraldehyde/2% paraformaldehyde in 0.1M } cacodylate buffer, 1% osmium post-fix, 1% uranyl acetate en bloc stain, } ethanol dehydration, and Spurr's/EPON resin. We are currently trying 2.5% } gluteraldehyde in cacodylate (at 0.1 and 0.17M), as well as combining } microwave fixation with our standard procedures. } } Our current procedures---microwave and otherwise---work very well with } mouse retinal tissue, but cats are being contrary. If anyone has any } tried and true techniques, I'd be very grateful to hear about them. It } could save us tons of time and frustration as we develop a new set of } protocols. } } Thanks very much. } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-5414 } Email: tindallr-at-missouri.edu } Web: http://www.biotech.missouri.edu/emc/ } }
Is there someone who has or would be willing to provide me with 3mm diameter disk cut from optical microscope slip covers. 30-50 pieces would be nice. No problem paying a reasonable price for this service. Please contact me off line if you can help us out.
Bruce, contact www.ssoptical.thomasregister.com or (260)749-9614. Be specific in your requirements, as they are not a microscopy supplier.
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax (678)467-0012 mobile
This message is made of 100% recycled electrons. ----- Original Message ----- } From: Bruce Brinson {brinson-at-rice.edu} To: MSA Listserver {microscopy-at-sparc5.microscopy.com} Sent: Friday, August 23, 2002 5:31 PM
-----Original Message----- } From: Kuusisto, Ari [mailto:Ari.Kuusisto-at-PerkinElmer.com] Sent: Friday, August 23, 2002 9:36 AM To: MSA listserver
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Schmucker II Science Center, Room SS024 West Chester University of Pennsylvania South Church Street & Rosedale Ave. West Chester, PA, 19383 Phone & FAX: 610-738-0437 eMail: fmonson-at-wcupa.edu http://darwin.wcupa.edu/casi/
Hi,
I am sending this for a colleague of mine. He would be grateful if somebody can help him.
'Tetenal has been discontinued their camera varnish 100912. Does anyone know something similar varnish for gluing optical components?
__________________________________________________________ Mr Raimo Harju, M.Sc., Technology Lead, Photonics Instrument Development PerkinElmer Life Sciences Wallac Oy P.O.Box 10, FIN-20101 Turku, Finland Phone: +358 2 2678111, Direct: +358 2 2678224, Fax: +358 2 2678530 Mobile: +358 40 7171629 E-mail: raimo.harju-at-perkinelmer.com, Website:www.perkinelmer.com'
I am new to Scanning Electron Microscopy. We recently acquired a JEOL 5600LV. The specimens we work on are mainly diatoms and although we got a demonstration on how to operate, it is mainly up to me to try to find the ideal settings to obtain good imaging! So far I haven't been able to get sharp focused images at high magnification (} x5000). Is there anyone out there who is also working on diatoms and is willing to share experiences?
Thanks,
Renaat Dasseville Protistology & Aquatic Ecology Dept. Biology, University Gent Krijgslaan 281, S8 9000 Gent, B E L G I U M tel: +32 9 264 85 04 fax +32 9 264 85 99
If you can find an older version of Practical Electron Microscopy for Biologists, second ed. by Meek, 1976, check out pages 108 and 109, they may be of help.
Mannie Steglich Tech Dir E M Lab U T M D Anderson Cancer Center
We are in the market for automated filling equipment for the liquid nitrogen dewar of our EDS unit. Iím aware of the convenience having this device.... the fact that you do not have to remember to fill the unit, and vacation filling. I wonder what experience others may have on the downside of having this device, as well as a source for buying such a unit.
Stu Smalinskas Metallurgist SKF NATC 46815 Port Street Plymouth, Michigan 48170 (734) 414-6862
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Good morning, One of mine coworkers would like to find out the opprox. price for new SEM with same features as mine. EDS/WDS, backscattered detector, dot mapping, line profile.
Diatoms with cellular matter in place or skeletons?
John Twilley
Renaat Dasseville wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hello, } } I am new to Scanning Electron Microscopy. We recently acquired a JEOL } 5600LV. The specimens we work on are mainly diatoms and although we got a } demonstration on how to operate, it is mainly up to me to try to find the } ideal settings to obtain good imaging! So far I haven't been able to get } sharp focused images at high magnification (} x5000). Is there anyone out } there who is also working on diatoms and is willing to share experiences? } } Thanks, } } Renaat Dasseville } Protistology & Aquatic Ecology } Dept. Biology, University Gent } Krijgslaan 281, S8 } 9000 Gent, B E L G I U M } tel: +32 9 264 85 04 } fax +32 9 264 85 99
Because they activate automatically when unattended, they must allow for the rapid release of boil-off and displacement of gas that comes with refilling. This means that there is usually more of a problem with condensation and ice buildup near the vent, which necessarily does not fit as closely as a manual filler cap. Ice that falls into the cryostat and is constantly moved by nitrogen bubbles can become a cause of noise in the detector, increasing deadtime among other things. So you may exchange the need for frequent filling for the need to clean out the cryostat periodically.
There is also some chance that the valve will fail in the open position, leading to overflow. One consideration in that case is the possibility that someone responding to the malfunction in a confined or poorly ventilated area could be overcome by the lack of oxygen before they realized the danger.
John Twilley
Kestutis Smalinskas wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } We are in the market for automated filling equipment } for the liquid nitrogen dewar of our EDS unit. Iím } aware of the convenience having this device.... the } fact that you do not have to remember to fill the } unit, } and vacation filling. I wonder what experience others } may have on the downside of having this device, as } well as a source for buying such a unit. } } Stu Smalinskas } Metallurgist } SKF NATC } 46815 Port Street } Plymouth, Michigan 48170 } (734) 414-6862 } } __________________________________________________ } Do You Yahoo!? } Yahoo! Finance - Get real-time stock quotes } http://finance.yahoo.com
I use a JEOL 5800LV microscope. Though most of my subjects are conductive, I do - at times - use the LV feature for non-conductive samples. I assume diatoms are non-conductive and your question relates to using the LV mode.
A magnification of 5000X is a lot to ask from this instrument. To maximize resolution, important parameters to set are:
- High spot size. I set mine to maximum. - Use the smaller (150 micron) orifice for differential pressure. - Low working distance. - High accelerating voltage. - Low chamber pressure.
All these parameters need to be balanced against sample integrity, sample charging, and gain when acquiring an image.
Stu Smalinskas Metallurgist SKF NATC 46815 Port Street Plymouth, Michigan 48170 ph.(734) 414-6862
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I am having a problem attempting to scrape and collect primary macrophage grown on a 60mm plastic petri dish.
The cells are fixed in 4% paraformaldehyde, blocked with 0.05M glycine, buffer rinsed, then water rinsed. I am using a disposable cell scraper from Fisher and scraping them in a very small amount of water, almost dry. When I collect the water with a pipet and transfer to an eppendof tube to spin the pellet down, there seem to be no cells. I have tried spinning them very hard, or very long but to no avail. I use this protocol on other cell types and get wonderful pellets, with plenty of sample to work with.
It seems to be cell type specific and I was wondering if anyone had any ideas to help me get a usable, visible pellet. I am trying to do ultrathin cryosections, so I am slightly limited in my options.
Thanks in advance. Leslie
Leslie Gunther Cummins Analytical Imaging Facility Albert Einstein College of Medicine 1300 Morris Park Ave. Bronx, NY 10461 718-430-3547
Let's start by what your samples are like, what magnification and resolution you need, unless money is no object. What system are you flying at the moment?
Peter
-----Original Message----- } From: ATC SEM Laboratory [mailto:atcsem-at-earthlink.net] Sent: Monday, August 26, 2002 9:20 AM To: Microscopy-at-sparc5.microscopy.com
Good morning, One of mine coworkers would like to find out the opprox. price for new SEM with same features as mine. EDS/WDS, backscattered detector, dot mapping, line profile.
Years ago, I shot some images of diatoms, but I coated them with Au/Pd. Is there a good reason for not coating a diatom intended for SEM observation?
Ron ----- Original Message ----- } From: "Kestutis Smalinskas" {smalinskas-at-yahoo.com} To: {Microscopy-at-sparc5.microscopy.com} ; {renaat.dasseville-at-rug.ac.be} Sent: Monday, August 26, 2002 1:56 PM
Dear Colleagues,
I have developed a computer program to index electron diffraction patterns. IndED is a powerful tool for indexing the electron diffraction patters. For given lattice parameters, it calculates the most possible set of all symmetrically equivalent zones.
The demo programs can be downloaded from M & M software library or from the following home page: http://homepage.mac.com/benyam/
There is an EPMA job going at the University of Otago, Dunedin, New Zealand, for a geologist/microprobist. JXA-8600.
It's a Scientific Officer (ie non-academic) job, in the Department of Geology, ref GG02/581.
The website doesn't seem to work properly (at least for me) www.otago.ac.nz/jobs, but one can email the Human Resources Division katherine.van-der-vlier-at-stonebow.otago.ac.nz or the Head of Department, Prof Alan Cooper, alan.cooper-at-stonebow.otago.nz.
I thought I'd post this as over the years people have sometimes expressed an interest in working in NZ.
There are three EMPAs in the country - a 733 in Wellington, an 840a in Auckland, and the Otago 8600.
Applications close this Friday August 30, and don't forget that NZ is 12 hours ahead of GMT.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
I am writing about the terminology of crystallographic axis. The International Tables of Crystallography refers to the X-, Y-, Z-axes of a crystal with basis vectors a, b, and c along these axes, but not a-, b-, and c-axes. However, many authors use a-, b-, and c-axes in TEM papers instead of X, Y, and Z axes. I would like to know why electron crystallographers prefer to use the terminology of a, b, and c-axes. Is there any reasons related to crystallography or history of TEM? Who defined a, b, and c-axes?
I would appreciate any comments on the terminology.
Take a look at the VBS Safe Fill system. It can save as much as 50% LN2 over manual filling. And it is safe.
http://www.vbsflex.com/ADF10.pdf
gary g.
No financial interest.
At 06:06 AM 8/26/2002, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
We are looking to replace (upgrade) our digital image capture system on our Hitachi S570 SEM and would be interested in hearing from users of various systems.
What system do you have? Are you happy with it (performance and service-wise)? How much did it cost?
Many thanks for sharing your experience.
PS: commercial vendors are also invited to contact us as well.
John B.
############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
I used a JSM 840 SEM in the past for Diatom work and c as well as gold-palladium coating. We got beautiful results. In LV mode we did find with the ESEM fei that working distance and the correct vacuum is very important. Due to the beam scattering more and the detectors not being that efficient you will have to play a bit. Reduce working distance and surprisingly increase spot size. KV appeasers to prefer 12 above 10. In ESEM mode we got clear images at 20 000 times, even new students did not battle. I love the ESEM when it comes to biological samples.
Mr. S. H. Coetzee Electron Microscope Unit University of Botswana Private Bag 0022 Gabarone Botswana
-----Original Message----- } From: John Twilley [mailto:jtwilley-at-sprynet.com] Sent: Monday, August 26, 2002 4:51 PM To: Renaat Dasseville Cc: Microscopy Listserver
Renaat;
Diatoms with cellular matter in place or skeletons?
John Twilley
Renaat Dasseville wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hello, } } I am new to Scanning Electron Microscopy. We recently acquired a JEOL } 5600LV. The specimens we work on are mainly diatoms and although we got a } demonstration on how to operate, it is mainly up to me to try to find the } ideal settings to obtain good imaging! So far I haven't been able to get } sharp focused images at high magnification (} x5000). Is there anyone out } there who is also working on diatoms and is willing to share experiences? } } Thanks, } } Renaat Dasseville } Protistology & Aquatic Ecology } Dept. Biology, University Gent } Krijgslaan 281, S8 } 9000 Gent, B E L G I U M } tel: +32 9 264 85 04 } fax +32 9 264 85 99
We use a thermal printer (Sony Video Graphic Printer, Model UP-890MD) on our SEM. Last I heard, they have a Model 895 that cost $895, much less than the $6000 we paid four years ago.
I know its not a digital capture system youre asking about, but you may want to consider it. After the capital cost, prints are roughly 20 cents apiece, which can then be scanned.
I really like this system, since prints are nearly instantaneous with good resolution. The biggest convenience is that during my semming sessions I like to fire away with pictures, then pick and choose the images Ill present to my client.
Stu Smalinskas Metallurgist SKF NATC Plymouth, Michigan stu.smalinskas-at-skf.com
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We have a TOPCON SM-510 low vacuum SEM, it has a diffusion pump with an Edward's roughing pump. I have been having a problem with the info on the digital captured photo disappearing. The only way I can get the info to reappear is to shut the SEM down for about one hour then restarting it, I have called the repair people about that problem. Last weekend I shut the SEM down and when I tried to start it up Monday, no joy. the vacuum wouldn't go low enough to start. After checking the o-rings and vacuum hoses I noticed there were waves of oil in the vacuum hoses. After draining them and running some ethanol through the lines and changing the roughing pump vacuum pump oil I managed to get the SEM up and running. My question is about the roughing pump it has two plastic screw in lids, one to fill up the pump and the other one labeled ballast. What is the ballast lid for? Its spring loaded and can be left open or closed. I have been running the pump with it closed since that the way the SEM was set up, the company setup person didn't know what it was for either. Would this have anything to do with the oil back up? I suspect an o-ring slipped in the SEM valving system but I have always wondered what the ballast lid is for? Does anyone know? Terry Ellis Hallmark Cards Inc. tellis2-at-hallmark cards 816-545-6573
Hi Leslie, It will look like a silly question but have you looked at your "invisible pellet" under a microscope or just by looking at your tube and not seeing it? I remember working with cultured macrophages and while doing an immunolabeling "loosing" my sample (I could not see a pellet anymore at some point of the experiment). But after putting the "invisible pellet" on a slide (cytospots) and looking under a microscope the cells were there! For some reason they became invisible for the eyes but were still there. Emmanuelle
Have you thought about growing them on a coverslip and then processsing the cells intact--the final stage would involve inverting a beem capsule with epon on top of it, polymerizing it, and then using liquid nitrogen to pop the capsul off. Cells adhere nicely to the epon and then you can section.
Peggy Sherwood Lab Associate, Photopathology Wellman Laboratories of Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
} -----Original Message----- } From: Leslie Cummins [SMTP:gunther-at-aecom.yu.edu] } Sent: Monday, August 26, 2002 3:01 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: TEM - macrophage collection } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello Listers } } I am having a problem attempting to scrape and collect primary macrophage } grown on a 60mm plastic petri dish. } } The cells are fixed in 4% paraformaldehyde, blocked with 0.05M glycine, } buffer rinsed, then water rinsed. I am using a disposable cell scraper } from Fisher and scraping them in a very small amount of water, almost } dry. When I collect the water with a pipet and transfer to an eppendof } tube to spin the pellet down, there seem to be no cells. I have tried } spinning them very hard, or very long but to no avail. I use this } protocol } on other cell types and get wonderful pellets, with plenty of sample to } work with. } } It seems to be cell type specific and I was wondering if anyone had any } ideas to help me get a usable, visible pellet. I am trying to do } ultrathin } cryosections, so I am slightly limited in my options. } } Thanks in advance. } Leslie } } } } Leslie Gunther Cummins } Analytical Imaging Facility } Albert Einstein College of Medicine } 1300 Morris Park Ave. } Bronx, NY 10461 } 718-430-3547 } } http://www.aecom.yu.edu/aif/ }
I agree that your thermal printer is convenient solution to a problem. It is nice to get prints quickly and that cheaply. I also found the 895 you listed. I didn't check a lot of sites. The one I did check listed the unit for under $1400 which still beats your $6000 cost of 4 years ago.
First, I should say that not all old SEMs can support a video printer. Our JEOL-840A as first delivered did not have a video signal that could be digitized. We added an option that provided us a video signal, but it was pricey.
You also need to be available of the limitations of video printers. The printers may be capable of many pixels of resolution, but the limiting factor is the resolution and quality of the video signal. NTSC video only specifies about 500 lines of resolution. (Your printer specifies a maximum of 608 lines that it can render.) That is acceptable for many purposes, but most users will want more for their digitized SEMs.
There is also a quality issue. The signal-to-noise ratio for an SEM can be quite low when setup for high resolution imaging. Therefor, it is necessary to extend the acquisition time to integrate or average the signal over time. I don't know how that is done with your system. Our Hitachi has a digital frame store that can retain the entire image and display it at video speeds. But by the time we do that, we might as well use our regular digital means to record the image (Quartz PCI or Oxford Instruments AutoBeam). I know those systems can digitize with 8 bits of precision. I don't know if the video signal can afford that much precision. (I understand the Sony printer is spec'ed for 256 gray levels, 8-bits, assuming that such a good signal is provided to it.
Scanning presents another problem. It means one more link in the chain on the way to digital. Now there is the question of S/N in the SEM signal and its integration on the SEM side, the transmission of that signal to the Sony printer, the digitization of that signal by the printer, the faithful rendition of that level on the printer paper, and the digitization of that printed signal by a scanner. That is a LOT of steps. A normal digitization approach catches the signal early on in the SEM avoiding the other steps which can introduce errors.
Having said all that, the video printer sounds quite convenient and may work for some users, but it should probably not be classed as a digitization system.
Warren
At 05:56 AM 8/27/02 -0700, you wrote:
} John: } } We use a thermal printer (Sony Video Graphic Printer, } Model UP-890MD) on our SEM. Last I heard, they have a } Model 895 that cost $895, much less than the $6000 we } paid four years ago. } } I know it's not a digital capture system you're asking } about, but you may want to consider it. After the } capital cost, prints are roughly 20 cents apiece, } which can then be scanned. } } I really like this system, since prints are nearly } instantaneous with good resolution. The biggest } convenience is that during my semming sessions I like } to "fire away" with pictures, then pick and choose the } images I'll present to my client. } } Stu Smalinskas } Metallurgist } SKF NATC } Plymouth, Michigan } stu.smalinskas-at-skf.com
The ballast, as I recall, is essentially a leak valve that leaks into the first stage of the pump so that there is a constant flow of gas through the pump. The idea is that this flow keeps down the concentration of condensible gasses/fluids into the pump oil. Opening the ballast will increase the ultimate pressure of the vacuum attainable by your roughing pump, which is why it us usually kept closed unless needed. From your description, however, it sounds like the valve that has failed on your pump is the anti-suckback valve. That is supposed to seal the pump off from your roughing line vacuum when the pump shuts down; if this doesn't work, then the pressure difference between the air outside and the line vacuum inside ends up blowing most of the oil out of your pump and into your roughing line, just as you've seen. If you can stand keeping your roughing line under vacuum, then a decent short-term approach is to simply hardwire your roughing pump so it always runs; this keeps vacuum on both sides of the valve, so no suckback. The long term solution, however, is to fix the valve or replace the pump.
Cheers, Ben Simkin (simkin-at-egr.msu.edu) Michigan State University dpt. Chem. Eng. and Materials Science
Once again I am most impressed with this list. I have received a pile of good information on this topic and now merely {g} have to sift through the many options I have been presented and make a good choice. Gladly this list has provided me with the many good options as well as information to use in making the final decision.
Thanks!
Richard Shalvoy Arch Chemicals, Inc. 350 Knotter Drive Cheshire, CT 06410 (203) 271-4394 rbshalvoy-at-archchemicals.com
I'm attempting to view cross sections of feather barbs on the SEM. We need to analyze nano vacuole diameters. However, cutting the barbs with a blade smears the keratin at the surface, thus covering the vacuoles that we need to collect data from.
I havn't tried crude freeze fracture because I need a flat surface.
One procedure recomends infiltrating with styrene-methacrylate, sectioning a flat surface with a diamond knife and then removing the resin with toluene and acetone.
Has anyone out there worked with this resin?
Where can I find it?
Help! Tim Quinn University of Kansas Program Assistant/Microscopists/Histo-Lab Lab Manager Ornithology Dept. Natural History Museum and Biodiversity Research Center Dyche Hall Room 414 Lawrence, KS 6604-2454 785-864-4556/785-331-4107 tquinn-at-ku.edu
Dear Terry, The ballast valve on a rotary pump lets a small amount of air into the pump, the same as if you had a small leak in your system. It is used to clean solvents or water vapour out of the oil in the pump by bubbling air throught the oil for about 10 to 15 minutes. It is only used on brand new oil (when you change the oil in the pump) or when you suspect the oil has been exposed to a solvent. Leave it open for no more than 15 minutes. It will degrade the ultimate vacuum slightly, while it is open. For normal operation, leave it closed. I cannot see how this would have anything to do with your digital image information. That is probably a software switch somewhere that "burns" the info into the digital photo and that has not been set. Oil in the foreline means you are getting bad backstreaming into the system. I would recommend a foreline trap to prevent that. It can cause a lot of problems in the vacuum system. I also never turn the microsope off, except for maintenance. I just turn off the displays and monitors. At 07:59 AM 08/27/2002 -0500, you wrote: } We have a TOPCON SM-510 low vacuum SEM, it has a diffusion pump with an } Edward's roughing pump. I have been having a problem with the info on the } digital captured photo disappearing. The only way I can get the info to } reappear is to shut the SEM down for about one hour then restarting it, I } have called the repair people about that problem. Last weekend I shut the } SEM down and when I tried to start it up Monday, no joy. the vacuum } wouldn't go low enough to start. After checking the o-rings and vacuum } hoses I noticed there were waves of oil in the vacuum hoses. After draining } them and running some ethanol through the lines and changing the roughing } pump vacuum pump oil I managed to get the SEM up and running. } My question is about the roughing pump it has two plastic screw in } lids, one to fill up the pump and the other one labeled ballast. What is } the ballast lid for? Its spring loaded and can be left open or closed. I } have been running the pump with it closed since that the way the SEM was } set up, the company setup person didn't know what it was for either. Would } this have anything to do with the oil back up? I suspect an o-ring slipped } in the SEM valving system but I have always wondered what the ballast lid } is for? Does anyone know? } Terry Ellis } Hallmark Cards Inc. } tellis2-at-hallmark cards } 816-545-6573 } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mmcconne-at-engineering.uiowa.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, August 27, 2002 at 12:46:49 ---------------------------------------------------------------------------
Email: mmcconne-at-engineering.uiowa.edu Name: Michael McConney
Organization: University of Iowa
Education: Undergraduate College
Location: Iowa City, IA
Question: I was wondering if you had suggestions on types of microscopy to use. The project I am working on involves bonding collagen IV to a linker chemical which is bonded to a polymer surface. The collagen bonding is all over the surface of the polymer. I am interested in obtaining the conformation of the collagen on the surface of the polymer, not the bending of the protein, but the geometry of the arranged collagen on the surface. We have used atomic-force techniques with mixed succes. I was wondering if you knew of any alternatives we could use?
You might try thin CA (cyanoacrylate).....crazy glue, super glue, etc. But this is the thin style. Hobby shops have it.
gary g.
At 11:46 AM 8/27/2002, you wrote:
} Dear Listies, } } I'm attempting to view cross sections of feather barbs on the SEM. We need } to analyze nano vacuole diameters. However, cutting the barbs with a blade } smears the keratin at the surface, thus covering the vacuoles that we need } to collect data from. } } I havn't tried crude freeze fracture because I need a flat surface. } } One procedure recomends infiltrating with styrene-methacrylate, sectioning } a flat surface with a diamond knife and then removing the resin with toluene } and acetone. } } Has anyone out there worked with this resin? } } Where can I find it? } } Help! } Tim Quinn } University of Kansas } Program Assistant/Microscopists/Histo-Lab Lab Manager } Ornithology Dept. } Natural History Museum and Biodiversity Research Center } Dyche Hall Room 414 } Lawrence, KS 6604-2454 } 785-864-4556/785-331-4107 } tquinn-at-ku.edu
Why not use Scion Image (free updated version of NIH Image) and purchase a Scion frame grabber board?
gary g.
At 05:09 AM 8/27/2002, you wrote:
} I am looking for a video frame grabber card for a PC (PCI slot) that will do } integration and work with NIH Image. Thanks in advance for your help. } } Rich Fiore, EM & SPM } NC State University } Analytical Instrumentation Facility } 2410 Campus Shore Drive } 318 EGRC, Campus Box 7531 } Raleigh, NC 27695 } Tel: 919-515-2348 } Fax: 919-515-6965 } Email: rich_fiore-at-ncsu.edu }
The only version of NIH Image that I know of is "Scion Image." To work within Scion Image, you need a Scion framegrabber, probably something like their CG-7 board, which sells for about $1700.
Of course, you could get another framegrabber board that would allow you to capture images, then with Scion Image running you could open the captured pic to work with it. I think you need either a .bmp or .tif file format to work in Image in this fashion.
We've had good luck with Integral framegrabber boards. They make two versions, the only difference is the types of video input accepted. Their FlashBus MV Pro (about $895) accepts RGB, Composite Video and S-Video (a.k.a. Y/C video). The FlashBus MV Pro Lite (about $600) accepts Composite Video and S-Video. Other framegrabbers are also available from Integral that have multiple inputs, if you need to run more than one device (scanner + camera, etc.)
Between used and new, the cost could range between $1 to $500K,. not including shipping. Your post is rather ambiguous relative to exactly what you are looking for. Is this a SEM or FESEM? Big difference. Turbo or diff pump?, etc., etc., blah, blah.
gg
At 06:19 AM 8/26/2002, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hallo Folks, Here's a baffling thing for you to mull over. I'm after some pointers after scratching my head for a couple of days. Here goes. We have a JEOL 733 with 4 WD spectrometers. We've the simple task of analysing some olivine crystals for their major (Si, Mg, Fe) and trace (Ca, Ni, Mn) elements. This should be a very simple, rudimentary and stressless thing to do, and normally is. The sad thing is, things aren't going normally. Standardisation is as simple as possible with as few standards as possible, and checked rigorously using materials for this purpose mounted in our standard block. However, when we go to the actual unknowns, Fe is sytematically about 3 wt % lower than expected for a stoichiometric olivine assuming the Mg and Si are ok. We can make this assumption because for an olivine of a specified SiO2 content we would expect a specified MgO content, and this checks out. For the given FeO content we would expect lower MgO and SiO2 contents, and this would simply make the totals worse. I've checked out the hardware and the fact that the calibration works for unknowns on the standard block suggests to me that there is no problem with the analytical protocol we're using. I considered C coating but thought that this is more likely to affect attenuation of the lower energy X-rays such as Si and Mg. Has anyone some suggestions on what the cause of this strange behaviour for a very simple mineral could be? Thanks, Malc.
-- Dr MP Roberts Phone: [+27](0)46 603 8313 Dept of Geology Fax: [+27](0)46 622 9715 Rhodes University Cell: 083 4060 262 (try your luck!) 6140 Grahamstown e-mail: m.roberts-at-ru.ac.za SOUTH AFRICA
This question is a little off the norm but I thought someone may have done this before.
I am looking for a math routine that with take raw x-ray data in text format (counts vs. deg angle) and process it to give me the following:
1) replot of the curves 2) identify peak locations (angle) 3) do a background subtraction 4) find the area under the peak curves
I understand that this could possibly be done with Microsoft Excel which would be ideal. My calculus is a bit fuzzy so any help would be greatly appreciated.
Roy Beavers Southern Methodist University Department of Geological Sciences P.O. Box 750395 Dallas, Tx. 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-mail.smu.edu
Another option is to buy a Matrox Meteor-2 grabber ($545) and then import the captured image to Scion or any other compatible program.
gary
At 09:14 PM 8/27/2002, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I am looking into getting digital output from a Hitachi SN-2460 SEM. Has anyone out there converted this particular scope? Any suggestions?
Another idea a colleague and I tossed around was upgrading the EDS system and using it as a source of digital image files. We currently have a Noran Voyager system with a Sun Microsystems workstation. I am sure that a Windows based system is now available-does anyone know if it is possible, and at what price, to simply buy a new computer and software package and connect it to the existing detector? Any suggestions would be welcome.
Sincerely,
Todd
Todd A. Kostman, Ph.D. Assistant Professor of Biology and Microbiology Director, Electron Microscopy Facility University of Wisconsin Oshkosh 800 Algoma Blvd Oshkosh, WI 54901-8640 ph; (920) 424-7301 fax: (920) 424-1101
This question is relevant to light microscopes in general, but I am posing it in the context of epifluorescence microscopy using a fiber-optic light guide and imaging using a square CCD array:
The image of a uniform field will be brightest near the center and fade away at the edges and corners. Why is this?
I assumed this variation in brightness was due to uneven illumination caused by a finite light source and the design of the illuminator optics. However, a co-worker recently showed me an article that mentioned vignetting in astronomical telescopes---uniformly bright objects appear brighter in the center of the field because the cone of rays the telescope can accept from an on-axis object is larger than that from an off-axis object. To what extent might vignetting cause uneven illumination in light microscopes? To what extent might vignetting result in brightness variation in the image of a uniformly illuminated field?
} Date: Tue, 27 Aug 2002 16:19:31 -0400 } To: "Terry E Ellis" {tellis2-at-hallmark.com} } From: Wil Bigelow {bigelow-at-engin.umich.edu} } Subject: Re: SEM roughing pump problem } Cc: } Bcc: } X-Attachments: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-- Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan 3062 Dow Bldg.; 2300 Hayward St. Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
Does Boeckeler Instruments have anyone local to St. Louis, MO that can do preventative maintenance on RMC MT-X or MT-XL ultramicrotomes?
Does anyone know of any third-party technicians that will service them in this area?
I'll contact Boeckeler, but I'd like to know if I have any other options.
Thanks,
Jaclynn M. Lett Staff Technician, Electron Microscopy Core Facility Fay and Carl Simons Center for Biology of Hearing and Deafness Harolds W. Siebens Hearing Research Center Central Institute for the Deaf 4560 Clayton Ave. St. Louis, MO 63110
We would like feedback on digital camera systems being used on TEM's in Diagnostic Pathology service labs. We have a growing Renal pathology service and want to utilize digital imaging to decrease turn around time and increase throughput. We have a lot of literature from vendors but need "real world" experiences. Please reply directly to:
Mike Goheen Dept. of Pathology & Lab Medicine Indiana University School of Medicine
This is primarily directed at people who do TEM, though it could well apply to other histologists and confocal-users as well. For many years I was an EM (and other kinds of imaging) technician, and a friend of mine still is. In the last few years we have both developed something that seems like RSI. In both cases, the disc between cervical vertebrae 5 and 6 is compressed, causing inflamed tissue to impinge on the nerve. This causes a great deal of pain in the area around the right scapula and in the right shoulder and arm, with a focal point at the top of the forearm. Do others on this list have the same problem? If it is common among EM and microscopy people, and not in the rest of the population, it could be the result of the stresses involved in sectioning, various kinds of microscopy, use of computers for graphic work etc., for hours at a time. I am asking mainly out of curiosity, but if it does turn out to be an occupational hazard, then people could try to prevent it - it really is quite unpleasant. Sorry about the cross-posting, but I think the overlap among the groups is not 100%.
I know that Emispec Systems, Inc. has a PC based imaging/analyzer that has been interfaced to this particular model instrument. (www.emispec.com). You can directly plug your Noran detector into their digital pulse processor and acquire digital images and x-ray maps (spectrum imaging) into a Windows based computer.
Disclaimer: I have no financial interest in this company; just a satisfied user.
Bob Roberts EM Lab Services, Inc. 449 NW 62nd St Topeka, Kansas 66617-1780 785.246.1232 www.emlabservices.com
By X-ray data I assume you mean X-ray Diffraction data. There is a web page that has a bunch of diffraction software (http://www.ccp14.ac.uk/solution/peakprofiling/) There are links to free and commercial software.
Sincerely, James Talbot
K/T GeoServices, Inc. Bulk and Clay Mineralogy by X-ray Diffraction Argyle, Texas (940) 597-9076 web site: http://www.ktgeo.com/
Beavers, Roy wrote:
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Hi, everyone, Does anybody know if there is cryo-electron microscopy facility near the DC-Baltimore area? Such as in UMaryland, Upenn, Johns Hopkins, NIH, HHMI, Delaware, Virginia and etc.? We want to do some kind of this work, and looking for collaborators.
I have done as you mentioned on a Hitachi S570. That is, used the EDX system's imaging and beam control as a capture vehicle. The only issue at the time was the resolution of the captured image relative to acquisition time since the processing capability was based on an old microprocessor and motherboard in the EDX hardware. In the older SEMs such as the Hitachi S570, there is no readily available port to grab the horizontal and vertical scan information and hence the video board had to be physically spliced into and the electronic levels made compatible with the EDX electronics. It was a messy affair but ultimately did work. The system worked on a Win95 platform.
The cost to do this on your particular microscope will likely be a function of who will be doing it, EDX vendor or graduate student. If you have an electrical engineering dept., you may want to tap into that resource for a good EE type person that understands analog/digital interfaces.
Hope this is of some aid.
Peter Tomic
-----Original Message----- } From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu] Sent: Wednesday, August 28, 2002 12:58 PM To: Microscopy-at-sparc5.microscopy.com
Greetings all,
I am looking into getting digital output from a Hitachi SN-2460 SEM. Has anyone out there converted this particular scope? Any suggestions?
Another idea a colleague and I tossed around was upgrading the EDS system and using it as a source of digital image files. We currently have a Noran Voyager system with a Sun Microsystems workstation. I am sure that a Windows based system is now available-does anyone know if it is possible, and at what price, to simply buy a new computer and software package and connect it to the existing detector? Any suggestions would be welcome.
Sincerely,
Todd
Todd A. Kostman, Ph.D. Assistant Professor of Biology and Microbiology Director, Electron Microscopy Facility University of Wisconsin Oshkosh 800 Algoma Blvd Oshkosh, WI 54901-8640 ph; (920) 424-7301 fax: (920) 424-1101
If you mean analysis of XRD data ask around about a piece of sofware called Jade. I think it might be able to do these things. It can certainly import the data in a wide variety of different formats.
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Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457 Ansto Materials Division Fax: 61-2-9543-7179 PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au Australia www: http://www.ansto.gov.au/
Please look at our web site: {http://www.yt-economy.com/senke.htm} Best regards Zhang Qixing address: nantong road no.71 Yantai China Yantai senke co.ltd tel:86-0535-6675831 fax:86-0535-6675830 post code:264000 email:yt-zqx-at-sohu.com
Please look at our web site: {http://www.yt-economy.com/senke.htm} Best regards Zhang Qixing address: nantong road no.71 Yantai China Yantai senke co.ltd tel:86-0535-6675831 fax:86-0535-6675830 post code:264000 email:yt-zqx-at-sohu.com
} ... } ... We've the simple task of } analysing some olivine crystals for their major (Si, Mg, Fe) and trace } (Ca, Ni, Mn) elements. using materials for this } ... However, when we go to the actual } unknowns, Fe is sytematically about 3 wt % lower than expected for a } stoichiometric olivine assuming the Mg and Si are ok. ... } ... } I considered C coating but thought that this is more likely to affect } attenuation of the lower energy X-rays such as Si and Mg. } ...
If you suspect the carbon coating at all, you might suspect charging which will attenuate Fe more than the lighter elements. I have to admit, olivines are not known to be a polishing or coating problem, but there may be higher resistances at grain bountries. What beam current are you using? You might try something lower than reccommended (e.g., ~ 5nA) just for isolating this symptom.
cheerios ... shAf :o) Avalon Peninsula, Newfoundland www.micro-investigations.com (in progress)
} From you post, I infer that you are performing the analysis on uncoated insulating material at high vacuum. If these assumptions are correct, your error may be from specimen charging.
One important parameter in quantitation is the incident beam potential (kV). If your specimen is charging, the potential (kV value) of the charge will reduce the effective potential of the original beam.
The charge induced reduction is often variable and difficult to accommodate in calculations. The reduced *effective* kV can lower the Fe K lines response below theoretical for an unaffected beam. Lower energy lines from lighter elements would be affected less. The result would be lower than actual/expected values for Fe concentration.
For the best result, although not always practical, the standards and the specimen should have the same carbon coating thickness/density. This is often achieved by coating the standards and the unknown at the same time, adjacent to one another (for even distribution - depends on coater).
Woody White McDermott Technology Inc. McD: http://www.mtiresearch.com/ Mine: http://woody.white.home.att.net
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Hallo Folks, Here's a baffling thing for you to mull over. I'm after some pointers after scratching my head for a couple of days. Here goes. We have a JEOL 733 with 4 WD spectrometers. We've the simple task of analysing some olivine crystals for their major (Si, Mg, Fe) and trace (Ca, Ni, Mn) elements. This should be a very simple, rudimentary and stressless thing to do, and normally is. The sad thing is, things aren't going normally. Standardisation is as simple as possible with as few standards as possible, and checked rigorously using materials for this purpose mounted in our standard block. However, when we go to the actual unknowns, Fe is sytematically about 3 wt % lower than expected for a stoichiometric olivine assuming the Mg and Si are ok. We can make this assumption because for an olivine of a specified SiO2 content we would expect a specified MgO content, and this checks out. For the given FeO content we would expect lower MgO and SiO2 contents, and this would simply make the totals worse. I've checked out the hardware and the fact that the calibration works for unknowns on the standard block suggests to me that there is no problem with the analytical protocol we're using. I considered C coating but thought that this is more likely to affect attenuation of the lower energy X-rays such as Si and Mg. Has anyone some suggestions on what the cause of this strange behaviour for a very simple mineral could be? Thanks, Malc.
-- Dr MP Roberts Phone: [+27](0)46 603 8313 Dept of Geology Fax: [+27](0)46 622 9715 Rhodes University Cell: 083 4060 262 (try your luck!) 6140 Grahamstown e-mail: m.roberts-at-ru.ac.za SOUTH AFRICA
Scott, I was only looking for rough estimates. Here the info that I've received:
***************************************************************** Without knowing the model numbers of your equipment, it is not possible to give an exact estimate. I also do not know the seriousness of their interest.
I would guess the cost to be in the range of $250-400K. It depends on options as much as anything. I think decent SEMs can be purchased for around $150K, more if they are fitted with a FE gun. EDS can cost another $50-100K. I am less familiar with WDS. We had a system that cost $60K for a single spectrometer. I don't know what current prices are. ******************************************************** Here is my guess
New SEM $100K (base modle) EDS $50K WDS $70K BSE $10K *************************************************************
Pavel, You dont say what model or manufacture your microscope is, are you looking for replacement cost or a brandnew sem with basic features that you have listed. If the latter I would say a reasonable estimate for a sem with features you have noted would be in the order of 370K, depending on manufacturer and stage and chamber requirements,ie 100-120k for wds, -at-60k for eds, 150k for sem bsd Regards Mike Webber *************************************************************** Hello Pavel,
Since we are a commercial vendor of SEMs and accessories, I thought it would be more appropriate to answer your question off-line. First, I hope I am not being out of line by answering you and apologize if so. I will not try to sell you an SEM! Just give you a straightforward answer.
We represent (sell and service) SEMs from both CamScan Electron Optics in the UK, and Tescan, located in Brno, Czech Republic. The full range of SEMs that these companies manufacture range in price from about $75,000 list price to about $300,000 list price. The three main factors which affect the price of an SEM are:
1) Chamber and stage design: Large chamber SEMs are significantly more expensive, not only because the cost of the larger chamber itself, but also the cost of the larger vacuum system required to pump it. Stages can be simple in design, with fewer motions (e.g. X and Y translation only), or fully eucentric 6-axis designs with automated (motorized) control.
2) Conventional high vacuum SEM or Variable Pressure SEM (also called 'Low Vacuum' by some, or environmental SEM if it operates at a high enough pressure to image water stably at room temperature). Variable pressure SEMs have more complex vacuum system designs and are therefore more expensive (typically about $10,000 to $20,000 more than the conventional counterpart).
3) Electron source type: conventional thermionic (filaments are heated to high temperatures to emit electrons) tungsten hairpin guns are the cheapest and have the lowest performance in terms of beam brightness, ultimate resolution, and image S/N ratio. Thermionic LaB6 (Lanthanum Hexaboride) guns have about 5-10 times the beam brightness of tungsten and are generally about $10,000-$20,000 more expensive (they also require better gun vacuum and usually have independent ion pumps and gun isolation valves). Field Emission electron sources are the highest performance and these SEMs are significantly more expensive than the thermionic types. FESEMs have 100 times the brightness of tungsten guns, very good ultimate resolution, and very high image S/N. If you must work regularly at high magnifications (e.g. 50,000X to 100,000x or higher), especially at low kV's, then a FESEM is for you.
Adding a backscattered electron detector to a SEM will generally cost about $7500 to $15,000 depending on the detector type and vendor. Most variable pressure SEMs should include this detector since a conventional Everhart-Thornley secondary electron detector is unusable in the vacuum range of VPSEMs. You can also buy special low vacuum secondary electron detectors from most vendors which are capable of providing SE images in variable pressure mode - they are usually about $12,000 to $15,000.
To finish the answer, EDS systems are almost always in the range of $40,000 to $80,000 for new systems with light element detectors. For $50,000 or less, you can choose from among many commercial systems which will provide good energy resolution, qualitative elemental analysis, quantitative elemental analysis, and digital Xray mapping/line profile capabilities (many provide full spectrum mapping for this price). WDS systems are more complex and therefore more expensive, usually in the range of $120,000 or so for a full-range spectrometer (Noran sells a smallerand less expensive spectrometer which is optimized for only a few elements, which you can choose).
You can probably improve on the separate EDS/WDS prices by buying an combined or integrated system, available from some of the companies (e.g. Oxford Instruments, Noran, and I believe Edax has just introduced a WDS system).
I hope this has helped some. If you wish, please feel free to contact me offline if you have more questions. ------------------------------------------------------------------
} One of mine coworkers would like to find out the opprox. price for new SEM } with same features as mine. } EDS/WDS, backscattered detector, dot mapping, line profile.
Best regards,
Tony mailto:towens-at-camscan-usa.com
Tony Owens CamScan USA Inc. 508 Thomson Park Dr. Cranberry Twp., PA 16066 Tel: (724) 772-7433 Fax: (724) 772-7434 URL: www.camscan-usa.com *********************************************************************** Pavel -
You need to be more specific. The range is quite large. Is your gun W, LaB6, FEG, SFEG?
Load lock? Polaroid/Hi-Res monitor? System volume? Low Vac, ESEM, Enviro? Photo-printer? 3, 4, 5, or 6-axis stage? Motorized?
etc......
All of these can add large amounts of $.
JQ ********************************************************************** Between used and new, the cost could range between $1 to $500K,. not including shipping. Your post is rather ambiguous relative to exactly what you are looking for. Is this a SEM or FESEM? Big difference. Turbo or diff pump?, etc., etc., blah, blah.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi Leslie, } It will look like a silly question but have you looked at your "invisible pellet" under a microscope or just by looking at your tube and not seeing it? I remember working with cultured macrophages and while doing an immunolabeling "loosing" my sample (I could not see a pellet anymore at some point of the experiment). But after putting the "invisible pellet" on a slide (cytospots) and looking under a microscope the cells were there! For some reason they became invisible for the eyes but were still there. } Emmanuelle } } Emmanuelle Roux, PhD } Senior Scientist } Caprion Pharmaceuticals } 7150 Alexander Fleming } St-Laurent, H4S 2C8 } Quebec, Canada } Tel: 514-940-3600 ext. 3773 } Fax: 514-940-3620 } }
Eastman Chemical Company has a position in its corporate research and development labs for a microscopist. Extensive experience with both optical and electron microscopy is required and experience in AFM, computer programming, and/or particle size analysis would be a strong plus. Knowledge of polymer morphology and/or the morphology of coatings and inks is also highly desirable. A PhD is preferred. The laboratory is well equipped with several optical microscopes, an SEM, a TEM, an AFM, and a particle size analyzer. The successful candidate will be working with this equipment and other scientists and engineers at Eastman to develop new/improved polymers and chemicals and to help solve manufacturing problems. Candidates must be highly motivated, have good communication skills and be able to work with teams.
Eastman Chemical Company, a Fortune 500 company, is a major manufacturer of plastics, fibers, and specialty organic chemicals. This position is at the Kingsport, TN site. Kingsport is located in northeast Tennessee in the foothills of the Smoky Mountains. It is Eastman's policy to provide equal opportunity for all qualified persons; to prohibit unlawful discrimination in employment practices, compensation practices, personnel procedures, and the administration of benefit plans and other programs; and to promote a full realization of equal employment opportunity through continuing affirmative action throughout company establishments. Reference Requisition No. 723 in your cover letter. Send CV/Resume to lmotz-at-eastman.com {mailto:lmotz-at-eastman.com} or to Eastman Chemical Company, PO Box 1975, B-215 - Staffing, Attn: Laurie Motz, Kingsport, TN 37662.
} Louis T. Germinario (Lou) } Physical Chemistry Research Laboratory } Eastman Chemical Company } Lincoln Street, B-150B } P.O. Box 1972 } Kingsport, TN 37662-5150 } (423) 229-4047 } (423) 229-4558 (Fax) } mailto:germ-at-eastman.com } }
Peter, I don't believe the original message is proposing the method you used. Many companies (including my own) provide EDX/imaging upgrades that replace the EDX system except for the detector and detector preamp. On most detectors, these upgrades are very easy to install.
Regarding the imaging, older SEMs vary even within the same model number. The Hitachi S570 is sometimes equipped to readily accept an active-scan system. We've seen others that require the installation of a Hitachi digital beam control box. With an active-scan system, older SEMs can acquire high-resolution digital images of amazing quality. Prices vary greatly and typically depend on the software requirements (i.e. acquisition and analysis, versus just acquisition).
Beth Gregory 4pi Analysis, Inc.
} Todd; } } I have done as you mentioned on a Hitachi S570. That is, used the EDX } system's imaging and beam control as a capture vehicle. The only issue at } the time was the resolution of the captured image relative to acquisition } time since the processing capability was based on an old microprocessor and } motherboard in the EDX hardware. In the older SEMs such as the Hitachi } S570, there is no readily available port to grab the horizontal and vertical } scan information and hence the video board had to be physically spliced into } and the electronic levels made compatible with the EDX electronics. It was } a messy affair but ultimately did work. The system worked on a Win95 } platform. } } The cost to do this on your particular microscope will likely be a function } of who will be doing it, EDX vendor or graduate student. If you have an } electrical engineering dept., you may want to tap into that resource for a } good EE type person that understands analog/digital interfaces. } } Hope this is of some aid. } } Peter Tomic } } -----Original Message----- } } From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu] } Sent: Wednesday, August 28, 2002 12:58 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: conversion of SN-2460 to digital } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
You can also try growing them on a 100 mm plate. I have done this after the osmification and using a rubber cell scraper, gently removed enough cells to get a visible pellet. Also check the plate under phase microscopy to ensure cell numbers.
Mike D.
"Sherwood, Margaret" wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Have you thought about growing them on a coverslip and then processsing the } cells intact--the final stage would involve inverting a beem capsule with } epon on top of it, polymerizing it, and then using liquid nitrogen to pop } the capsul off. Cells adhere nicely to the epon and then you can section. } } Peggy Sherwood } Lab Associate, Photopathology } Wellman Laboratories of Photomedicine (W224) } Massachusetts General Hospital } 55 Fruit Street } Boston, MA 02114 } 617-724-4839 (voice mail) } 617-726-6983 (lab) } 617-726-3192 (fax) } msherwood-at-partners.org } } } -----Original Message----- } } From: Leslie Cummins [SMTP:gunther-at-aecom.yu.edu] } } Sent: Monday, August 26, 2002 3:01 PM } } To: Microscopy-at-sparc5.microscopy.com } } Subject: TEM - macrophage collection } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hello Listers } } } } I am having a problem attempting to scrape and collect primary macrophage } } grown on a 60mm plastic petri dish. } } } } The cells are fixed in 4% paraformaldehyde, blocked with 0.05M glycine, } } buffer rinsed, then water rinsed. I am using a disposable cell scraper } } from Fisher and scraping them in a very small amount of water, almost } } dry. When I collect the water with a pipet and transfer to an eppendof } } tube to spin the pellet down, there seem to be no cells. I have tried } } spinning them very hard, or very long but to no avail. I use this } } protocol } } on other cell types and get wonderful pellets, with plenty of sample to } } work with. } } } } It seems to be cell type specific and I was wondering if anyone had any } } ideas to help me get a usable, visible pellet. I am trying to do } } ultrathin } } cryosections, so I am slightly limited in my options. } } } } Thanks in advance. } } Leslie } } } } } } } } Leslie Gunther Cummins } } Analytical Imaging Facility } } Albert Einstein College of Medicine } } 1300 Morris Park Ave. } } Bronx, NY 10461 } } 718-430-3547 } } } } http://www.aecom.yu.edu/aif/ } }
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Beth, all,
Considering the fact that the SEM already has an EDS system attached, I would guess, that it is already equipped with the digital beam control interface. That would probably allow to hook up any of the digital acquisition systems, yours, ours, etc.
A possibly more cost efficient way might be to talk to Noran and see if they have a migration possibility from this Unix system to a PC based system. Noran is using our software on some of their systems, so that might be a possibility to get image acquisition on a PC and some processing capabilities at the same time. However, depending on the age of the system, that might not be possible, or only with additional hardware changes.
If only image acquisition is required, our ADDA II can be operated in parallel to an existing EDS system (I am sure your system can do that also), with options do to dot mapping.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
******* Disclaimer ******** As the manufacturer of digital image acquisition systems we DO have an interest (financial and otherwise) in the products mentioned above. Please make sure to get all information before making a decision. **************************
-----Original Message----- } From: Beth Gregory [mailto:gregory-at-4pi.com] Sent: Thursday, August 29, 2002 8:41 AM To: Microscopy-at-sparc5.microscopy.com
Peter, I don't believe the original message is proposing the method you used. Many companies (including my own) provide EDX/imaging upgrades that replace the EDX system except for the detector and detector preamp. On most detectors, these upgrades are very easy to install.
Regarding the imaging, older SEMs vary even within the same model number. The Hitachi S570 is sometimes equipped to readily accept an active-scan system. We've seen others that require the installation of a Hitachi digital beam control box. With an active-scan system, older SEMs can acquire high-resolution digital images of amazing quality. Prices vary greatly and typically depend on the software requirements (i.e. acquisition and analysis, versus just acquisition).
Beth Gregory 4pi Analysis, Inc.
} Todd; } } I have done as you mentioned on a Hitachi S570. That is, used the EDX } system's imaging and beam control as a capture vehicle. The only issue at } the time was the resolution of the captured image relative to acquisition } time since the processing capability was based on an old microprocessor and } motherboard in the EDX hardware. In the older SEMs such as the Hitachi } S570, there is no readily available port to grab the horizontal and vertical } scan information and hence the video board had to be physically spliced into } and the electronic levels made compatible with the EDX electronics. It was } a messy affair but ultimately did work. The system worked on a Win95 } platform. } } The cost to do this on your particular microscope will likely be a function } of who will be doing it, EDX vendor or graduate student. If you have an } electrical engineering dept., you may want to tap into that resource for a } good EE type person that understands analog/digital interfaces. } } Hope this is of some aid. } } Peter Tomic } } -----Original Message----- } } From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu] } Sent: Wednesday, August 28, 2002 12:58 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: conversion of SN-2460 to digital } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cbc-at-post.queensu.ca) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday, August 29, 2002 at 11:02:32 ---------------------------------------------------------------------------
Email: cbc-at-post.queensu.ca Name: Charlie Cooney
Organization: Queens University
Education: Graduate College
Location: Kingston, Ontario Canada
Question: I have been doing some housekeeping and came across a bottle of Dr. Vogel's Sparbeize. My recollection is that this was an additive to an electropolishing solution for metals but I cannot remember what the chemical compostion of this stuff is or what solution it was use in. Does anyone know how to use this stuff and what the compositon is?
I need to find a good adhesive to use as for plastic serial sections. One recommendation was a product called Pattex diluted with xylene. I am unable to locate this product.
Any other suggestions for accomplishing good serial sections? I'm using a diamond histo knife with big boat.
Thanks, Tim Quinn University of Kansas Program Assistant/Microscopists Ornithology Dept. Natural History Museum and Biodiversity Research Center Dyche Hall Room 414 Lawrence, KS 6604-2454 785-864-4556/785-331-4107 tquinn-at-ku.edu
Sparbeize is indeed an additive to other etching solutions. It was produced by a German company. (Max Hoeck o.H.G. in Duesseldorf). It was used for etching mainly stainless steel samples with a high Cr content. eg. 10 ccs HNO3 0.30 Vogels Sparbeize 100 ccs HCL 100 ccs dist H2O Etching either at room temp or 50 degrees C. As I can remember, the actual ingredients of Sparbeize were kept secret.
Cheers
Hans Brinkies Professional Officer, Electron Microscopy and Metallography Swinburne, University of Technology School of Engineering and Science Industrial Microscopy Laboratory P.O.Box 218 - Hawthorn - Vic -3122 - Australia Phone: +61 3 9214 8657 Fax: +61 3 9214 8264 Email: Hbrinkies-at-swin.edu.au
Thanks to everybody who replied to my question. I started off answering each reply individually, but there are just so many that I hope everybody will accept this blanket gratitude. It seems to be a common problem among EM people and other microscopists, so perhaps it could count as an occupational injury. If it is, please everybody, take care to prevent it, as far as you can.
pattex is a subdivision of Henkel / Germany. - I do not know if their products are available outside of Germany ... Take a look at their website http://www.pattex.de, probably the "Kraftkleber" is the one you are looking for?
Greetings Gunnar
} From: "Quinn, Tim Lee" {tquinn-at-ku.edu} To: "'Microscopy-at-MSA.MIcroscopy.com'" {Microscopy-at-sparc5.microscopy.com}
Hi, You might want to check this paper: 3A critical evaluation of the results of the '92 round robin microanalysis test(EDS & Peels) performed by the Ile de Frande TEMnetwork, in MMM 4, p387-399. (multiple authors) Analysis protocols were discussed in the instance of Olivine. If you want a sample we usedyou should contact J. Ingrin (he is now somewher in Toulouse). Gilles
} } Hallo Folks, } Here's a baffling thing for you to mull over. I'm after some } pointers after scratching my head for a couple of days. Here goes. We } have a JEOL 733 with 4 WD spectrometers. We've the simple task of } analysing some olivine crystals for their major (Si, Mg, Fe) and trace } (Ca, Ni, Mn) elements. This should be a very simple, rudimentary and } stressless thing to do, and normally is. The sad thing is, things aren't } going normally. Standardisation is as simple as possible with as few } standards as possible, and checked rigorously using materials for this } purpose mounted in our standard block. However, when we go to the actual } unknowns, Fe is sytematically about 3 wt % lower than expected for a } stoichiometric olivine assuming the Mg and Si are ok. We can make this } assumption because for an olivine of a specified SiO2 content we would } expect a specified MgO content, and this checks out. For the given FeO } content we would expect lower MgO and SiO2 contents, and this would } simply make the totals worse. I've checked out the hardware and the fact } that the calibration works for unknowns on the standard block suggests } to me that there is no problem with the analytical protocol we're using. } I considered C coating but thought that this is more likely to affect } attenuation of the lower energy X-rays such as Si and Mg. } Has anyone some suggestions on what the cause of this } strange behaviour for a very simple mineral could be? } Thanks, } Malc. } } -- } Dr MP Roberts Phone: [+27](0)46 603 8313 } Dept of Geology Fax: [+27](0)46 622 9715 } Rhodes University Cell: 083 4060 262 (try your luck!) } 6140 Grahamstown e-mail: m.roberts-at-ru.ac.za } SOUTH AFRICA
-- _______________________________________________________ Gilles Hug LEM, UMR 104, ONERA-CNRS, BP72 92322 Chatillon Cedex, France tel : +33 1 46 73 45 42 fax : +33 1 46 73 41 55 mailto:Gilles.Hug-at-onera.fr http://www.onera.fr/lem/anachistr/index.html _______________________________________________________ Office National d'Etudes et de Recherches Aerospatiales ------- & ------ Centre National de la Recherche Scientifique _______________________________________________________
Could some of you please provide me some information regarding where to purchase a 21" or larger low frequency shielded computer monitor? I would like to buy one but having trouble to find a supplier. The monitor will be used to replace the smaller one on our Tecnai TEM. Your help is greatly appreciated.
Thank you, Ping Li
-- Ping Li, Ph.D. Director, Scientific Imaging Suite Department of Biology Dalhousie University Halifax, NS B3H 4J1 Canada
To adhere serial setions together into a ribbon while sectioning, I'v used (with success) a small amount of a product called Tackiwax can be applied to the sides of the blockface at the top and bottom of the trapezoid. I think the reference is in M.A. Hayat's book on EM Techniques for Biological Electron Microscopy.
I ordered a tub (1 lb. container - a lifetime supply for ultramicrotomy) from Fisher Scientific, but other suppliers may be able to get it for you. Here's information off of the label:
Dr. Heather A. Owen, Director Electron Microscope Laboratory Department of Biological Sciences University of Wisconsin - Milwaukee Lapham Hall, P.O. Box 413 Milwaukee, WI 53210 USA
Ladd makes it. It's very nice when using plastic culture dishes. I use the same recipe as for epon 812. Let me know if you need specifics. Mary Gail Engle
At 08:41 AM 8/30/02 -0400, Lesley S. Bechtold wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Mary Gail Engle Sr. Research Laboratory Manager Electron Microscopy & Imaging Facility Health Sciences Research Bldg. 001 University of Kentucky Lexington, KY 40536-0305
The Sony Multiscan E500 (21") and most all of the Multiscan series are shielded.
A better course might be to look into 20" flat panel LCDs. I'm using a 20" RGB+sync unit made in Germany with a NEC LCD display unit. It works quite well. NEC also makes some 18" LCD displays that are very nice. I'm not sure if they make larger ones. The 18" one I have used is the 1850 and is the standard LCD display on the FEI Sirion FEGSEM.
gary
At 04:55 AM 8/30/2002, you wrote:
} Could some of you please provide me some information regarding where to } purchase a 21" or larger low frequency shielded computer monitor? I would } like to buy one but having trouble to find a supplier. The monitor will be } used to replace the smaller one on our Tecnai TEM. Your help is greatly } appreciated. } } Thank you, } Ping Li } } -- } Ping Li, Ph.D. } Director, Scientific Imaging Suite } Department of Biology } Dalhousie University } Halifax, NS B3H 4J1 } Canada } } Tel: 902-494-3309 } Fax: 902-494-3736 } E-mail: Ping.Li-at-Dal.Ca
LX-112 is the EPON replacement sold by LADD. We use is routinely and follow the traditional Luft formulations.
Frank
At 08:41 AM 8/30/02 -0400, Lesley S. Bechtold wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi all I am researching salaries. The problem I am having is my job is primarily failure analysis, but I am a technician so most surveys list quality technician and quality engineer. I have responsibilities in both areas. Any help off or online would be most appreciated. 9 years experience.
Apologies for off topic discussion
Robert Fowler Quality Assurance Technician (Failure Analysis) TDK Components USA, Inc. Multilayer Ceramic Capacitor Division 1 TDK Boulevard Peachtree City GA 30269-2051 Telephone: (770) 631-0410 Ext.315 Fax: (770) 487-1460 email: rfowler-at-tdktca.com www.tdk.com
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If you are not the intended recipient, be advised that any use, dissemination, distribution or duplication of this transmission is strictly prohibited. If you received this transmission in error, please notify the sender immediately by electronic reply to this transmission or by phone (847-803-6100). Thank you.
LX-112 is a Ladd Research product. It is used in wide variety of projects from EM research to the aerospace industry. Please reply directly with more details on your project, check our web site, http://www.laddresearch.com, or call the number listed below and ask for Dr. Charles Duvic our chemist and you can discuss it with him.
Thank you,
John Arnott
Ladd Research 83 Holly Court Williston, VT 05495
On-line Catalog: http://www.laddresearch.com
tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US) fax: 1-802-660-8859 e-mail: sales-at-laddresearch.com ----- Original Message ----- } From: "Lesley S. Bechtold" {lsb-at-jax.org} To: {microscopy-at-sparc5.microscopy.com} Sent: Friday, August 30, 2002 8:41 AM
Ah....OK. Can you tell us what the basic premise was for doing this? No condemnation, just wondering why you are doing this exercise. As you can imagine, there is a huge range of options, systems and prices.
I'm a cat....curious.
gary g.
At 05:22 AM 8/30/2002, you wrote: } I was not looking for purchase! Just the info } } } ----- Original Message ----- } From: "Gary Gaugler" {gary-at-gaugler.com} } To: "ATC SEM Laboratory" {atcsem-at-earthlink.net} } Cc: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com} } Sent: Thursday, August 29, 2002 9:25 PM } Subject: Re: New SEM opproximate price } } } } Excellent!! what did you recommend for the purchase? } } } } gg } } } } At 04:07 AM 8/27/2002, you wrote: } } } } } I appreciate the information. I think, I've got more than enough. } } } Thank you all very much for comprehensive answers. } } } } } } Pavel } }
Heather A. Owen wrote: ============================================================
To adhere serial setions together into a ribbon while sectioning, I'v used (with success) a small amount of a product called Tackiwax can be applied to the sides of the blockface at the top and bottom of the trapezoid. I think the reference is in M.A. Hayat's book on EM Techniques for Biological Electron Microscopy.
I ordered a tub (1 lb. container - a lifetime supply for ultramicrotomy) from Fisher Scientific, but other suppliers may be able to get it for you. Here's information off of the label:
============================================================== Could someone explain how this product Tackiwax apparently a product of Boekel Scientific would differ from a good dental wax, such as on URL www.2spi.com/catalog/knives/cavex-set-up-dental-wax-sh.shtml
which is also used pretty widely in the world for ultramicrotomy applications.
I am not suggesting that this common (but very high quality) dental wax is better for this application, but it is far more readily available from a large number of suppliers (such as one of our many competitors like Ted Pella, Inc.). The Cavex® brand of dental wax, unlike a lot of the dental wax products on the market, is actually approved for use in dentistry in most countries around the world. What is not clear to me is whether soft, medium or hard would be the preferred hardness for this particular application (to adhere serial sections into a ribbon).
The cost to place a small order for a single item, for many customers, can be staggering, so I ask this question because if it is not any different than a good dental wax, such as the Cavex wax, it could keep people in certain countries from placing a small order (because the shipping and other costs of importation may be many times larger than the fob value of the item itself). This is inherently a low cost item and as was pointed out previously, one pack can last virtually a life time.
Disclaimer: SPI Supplies is a major supplier of high quality dental wax for use in microtomy applications worldwide so naturally we would have a vested interest in having customers order this wax from SPI along with their other needed items.
Chuck
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Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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I am looking for a replacement part for our poloron sputter/coater. It is a model 0E5000, manufacturing date unknown. The part that is broken is a hollow glass cylinder that has a rubber seal at either end and acts as the sputtering chamber. Any recommendations on a supplier or glass manufacturer is welcomed or other ideas! Thank you in advance.
Wil Kunkel curari-at-asu.edu student of chemistry This email was sent with 100.00% recycled electrons.
The Polaron (check spelling) supplier in the US should be able to get this item for you. If not, that would be a good reason to avoid this brand of coater.
Why did it fail? This seems to me to be a very low failure rate item. The rubber boots do need to be replaced at some interval (like on my Anatech Hummer VII). But having the glass cylinder crack would be really disturbing to me. Did you miss-treat it?
gary g.
At 05:36 PM 8/31/2002, you wrote:
} Dear Listers } } I am looking for a replacement part for our poloron } sputter/coater. It } is a model 0E5000, manufacturing date unknown. The part that is broken is a } hollow glass cylinder that has a rubber seal at either end and acts as the } sputtering chamber. Any recommendations on a supplier or glass } manufacturer is } welcomed or other ideas! Thank you in advance. } } Wil Kunkel } curari-at-asu.edu } student of chemistry } This email was sent with 100.00% recycled electrons.
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