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From: Germinario, L T - Eastman :      germ-at-eastman.com
Date: Thu, 29 Aug 2002 09:41:09 -0400
Subject: Microscopist Opening: Eastman Chemical Company

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{Microscopy-at-sparc5.microscopy.com}



Job Posting:

Microscopist Opening

Eastman Chemical Company has a position in its corporate research and
development labs for a microscopist. Extensive experience with both optical
and electron microscopy is required and experience in AFM, computer
programming, and/or particle size analysis would be a strong plus.
Knowledge of polymer morphology and/or the morphology of coatings and inks
is also highly desirable. A PhD is preferred. The laboratory is well
equipped with several optical microscopes, an SEM, a TEM, an AFM, and a
particle size analyzer. The successful candidate will be working with this
equipment and other scientists and engineers at Eastman to develop
new/improved polymers and chemicals and to help solve manufacturing
problems. Candidates must be highly motivated, have good communication
skills and be able to work with teams.

Eastman Chemical Company, a Fortune 500 company, is a major manufacturer of
plastics, fibers, and specialty organic chemicals. This position is at the
Kingsport, TN site. Kingsport is located in northeast Tennessee in the
foothills of the Smoky Mountains. It is Eastman's policy to provide equal
opportunity for all qualified persons; to prohibit unlawful discrimination
in employment practices, compensation practices, personnel procedures, and
the administration of benefit plans and other programs; and to promote a
full realization of equal employment opportunity through continuing
affirmative action throughout company establishments. Reference Requisition
No. 723 in your cover letter. Send CV/Resume to lmotz-at-eastman.com
{mailto:lmotz-at-eastman.com} or to Eastman Chemical Company, PO Box 1975,
B-215 - Staffing, Attn: Laurie Motz, Kingsport, TN 37662.


} Louis T. Germinario (Lou)
} Physical Chemistry Research Laboratory
} Eastman Chemical Company
} Lincoln Street, B-150B
} P.O. Box 1972
} Kingsport, TN 37662-5150
} (423) 229-4047
} (423) 229-4558 (Fax)
} mailto:germ-at-eastman.com
}
}


From daemon Thu Aug 29 09:50:49 2002



From: Beth Gregory :      gregory-at-4pi.com
Date: Thu, 29 Aug 2002 10:40:38 -0400
Subject: RE: conversion of SN-2460 to digital

Contents Retrieved from Microscopy Listserver Archives
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Peter,
I don't believe the original message is proposing the method you used. Many
companies (including my own) provide EDX/imaging upgrades that replace the
EDX system except for the detector and detector preamp. On most detectors,
these upgrades are very easy to install.

Regarding the imaging, older SEMs vary even within the same model number.
The Hitachi S570 is sometimes equipped to readily accept an active-scan
system. We've seen others that require the installation of a Hitachi
digital beam control box. With an active-scan system, older SEMs can
acquire high-resolution digital images of amazing quality. Prices vary
greatly and typically depend on the software requirements (i.e. acquisition
and analysis, versus just acquisition).

Beth Gregory
4pi Analysis, Inc.

} Todd;
}
} I have done as you mentioned on a Hitachi S570. That is, used the EDX
} system's imaging and beam control as a capture vehicle. The only issue at
} the time was the resolution of the captured image relative to acquisition
} time since the processing capability was based on an old microprocessor and
} motherboard in the EDX hardware. In the older SEMs such as the Hitachi
} S570, there is no readily available port to grab the horizontal and vertical
} scan information and hence the video board had to be physically spliced into
} and the electronic levels made compatible with the EDX electronics. It was
} a messy affair but ultimately did work. The system worked on a Win95
} platform.
}
} The cost to do this on your particular microscope will likely be a function
} of who will be doing it, EDX vendor or graduate student. If you have an
} electrical engineering dept., you may want to tap into that resource for a
} good EE type person that understands analog/digital interfaces.
}
} Hope this is of some aid.
}
} Peter Tomic
}
} -----Original Message-----
} } From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu]
} Sent: Wednesday, August 28, 2002 12:58 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: conversion of SN-2460 to digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Thu Aug 29 10:31:04 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Thu, 29 Aug 2002 11:13:19 -0400
Subject: Re: TEM - macrophage collection

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You can also try growing them on a 100 mm plate. I have done this after the
osmification
and using a rubber cell scraper, gently removed enough cells to get a visible
pellet. Also check
the plate under phase microscopy to ensure cell numbers.

Mike D.

"Sherwood, Margaret" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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}
} Have you thought about growing them on a coverslip and then processsing the
} cells intact--the final stage would involve inverting a beem capsule with
} epon on top of it, polymerizing it, and then using liquid nitrogen to pop
} the capsul off. Cells adhere nicely to the epon and then you can section.
}
} Peggy Sherwood
} Lab Associate, Photopathology
} Wellman Laboratories of Photomedicine (W224)
} Massachusetts General Hospital
} 55 Fruit Street
} Boston, MA 02114
} 617-724-4839 (voice mail)
} 617-726-6983 (lab)
} 617-726-3192 (fax)
} msherwood-at-partners.org
}
} } -----Original Message-----
} } From: Leslie Cummins [SMTP:gunther-at-aecom.yu.edu]
} } Sent: Monday, August 26, 2002 3:01 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: TEM - macrophage collection
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello Listers
} }
} } I am having a problem attempting to scrape and collect primary macrophage
} } grown on a 60mm plastic petri dish.
} }
} } The cells are fixed in 4% paraformaldehyde, blocked with 0.05M glycine,
} } buffer rinsed, then water rinsed. I am using a disposable cell scraper
} } from Fisher and scraping them in a very small amount of water, almost
} } dry. When I collect the water with a pipet and transfer to an eppendof
} } tube to spin the pellet down, there seem to be no cells. I have tried
} } spinning them very hard, or very long but to no avail. I use this
} } protocol
} } on other cell types and get wonderful pellets, with plenty of sample to
} } work with.
} }
} } It seems to be cell type specific and I was wondering if anyone had any
} } ideas to help me get a usable, visible pellet. I am trying to do
} } ultrathin
} } cryosections, so I am slightly limited in my options.
} }
} } Thanks in advance.
} } Leslie
} }
} }
} }
} } Leslie Gunther Cummins
} } Analytical Imaging Facility
} } Albert Einstein College of Medicine
} } 1300 Morris Park Ave.
} } Bronx, NY 10461
} } 718-430-3547
} }
} } http://www.aecom.yu.edu/aif/
} }



From daemon Thu Aug 29 12:25:50 2002



From: LT4725n42-at-aol.com
Date: Thu, 29 Aug 0102 16:01:04 +0100
Subject: Join The Fun ....

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From: LT4725n42-at-aol.com
Date: Thu, 29 Aug 0102 16:01:04 +0100
Subject: Join The Fun ....

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From daemon Thu Aug 29 14:22:11 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 29 Aug 2002 13:08:00 -0600
Subject: RE: conversion of SN-2460 to digital

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Beth, all,

Considering the fact that the SEM already has an EDS system attached, I
would guess, that it is already equipped with the digital beam control
interface. That would probably allow to hook up any of the digital
acquisition systems, yours, ours, etc.

A possibly more cost efficient way might be to talk to Noran and see if they
have a migration possibility from this Unix system to a PC based system.
Noran is using our software on some of their systems, so that might be a
possibility to get image acquisition on a PC and some processing
capabilities at the same time. However, depending on the age of the system,
that might not be possible, or only with additional hardware changes.

If only image acquisition is required, our ADDA II can be operated in
parallel to an existing EDS system (I am sure your system can do that also),
with options do to dot mapping.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

******* Disclaimer ********
As the manufacturer of digital image acquisition systems we DO have an
interest (financial and otherwise) in the products mentioned above. Please
make sure to get all information before making a decision.
**************************


-----Original Message-----
} From: Beth Gregory [mailto:gregory-at-4pi.com]
Sent: Thursday, August 29, 2002 8:41 AM
To: Microscopy-at-sparc5.microscopy.com


Peter,
I don't believe the original message is proposing the method you used. Many
companies (including my own) provide EDX/imaging upgrades that replace the
EDX system except for the detector and detector preamp. On most detectors,
these upgrades are very easy to install.

Regarding the imaging, older SEMs vary even within the same model number.
The Hitachi S570 is sometimes equipped to readily accept an active-scan
system. We've seen others that require the installation of a Hitachi
digital beam control box. With an active-scan system, older SEMs can
acquire high-resolution digital images of amazing quality. Prices vary
greatly and typically depend on the software requirements (i.e. acquisition
and analysis, versus just acquisition).

Beth Gregory
4pi Analysis, Inc.

} Todd;
}
} I have done as you mentioned on a Hitachi S570. That is, used the EDX
} system's imaging and beam control as a capture vehicle. The only issue at
} the time was the resolution of the captured image relative to acquisition
} time since the processing capability was based on an old microprocessor and
} motherboard in the EDX hardware. In the older SEMs such as the Hitachi
} S570, there is no readily available port to grab the horizontal and
vertical
} scan information and hence the video board had to be physically spliced
into
} and the electronic levels made compatible with the EDX electronics. It was
} a messy affair but ultimately did work. The system worked on a Win95
} platform.
}
} The cost to do this on your particular microscope will likely be a function
} of who will be doing it, EDX vendor or graduate student. If you have an
} electrical engineering dept., you may want to tap into that resource for a
} good EE type person that understands analog/digital interfaces.
}
} Hope this is of some aid.
}
} Peter Tomic
}
} -----Original Message-----
} } From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu]
} Sent: Wednesday, August 28, 2002 12:58 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: conversion of SN-2460 to digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Thu Aug 29 15:03:22 2002



From: cbc-at-post.queensu.ca ()
Date: Thu, 29 Aug 2002 14:52:30 -0500
Subject: Ask-A-Microscopist:what is Dr. Vogel's Sparbeiz

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cbc-at-post.queensu.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
August 29, 2002 at 11:02:32
---------------------------------------------------------------------------

Email: cbc-at-post.queensu.ca
Name: Charlie Cooney

Organization: Queens University

Education: Graduate College

Location: Kingston, Ontario Canada

Question: I have been doing some housekeeping and came
across a bottle of Dr. Vogel's Sparbeize. My
recollection is that this was an additive to
an electropolishing solution for metals but I
cannot remember what the chemical compostion of
this stuff is or what solution it was use in.
Does anyone know how to use this stuff and what
the compositon is?

Thanks


---------------------------------------------------------------------------


From daemon Thu Aug 29 17:06:27 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Thu, 29 Aug 2002 16:57:48 -0500
Subject: Adhesive for binding plastic serial sections

Contents Retrieved from Microscopy Listserver Archives
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Listies

I need to find a good adhesive to use as for plastic serial sections. One
recommendation was a product called Pattex diluted with xylene. I am unable
to locate this product.

Any other suggestions for accomplishing good serial sections? I'm using a
diamond histo knife with big boat.

Thanks,
Tim Quinn
University of Kansas
Program Assistant/Microscopists
Ornithology Dept.
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556/785-331-4107
tquinn-at-ku.edu



From daemon Thu Aug 29 18:45:33 2002



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au (by way of
Date: Thu, 29 Aug 2002 18:33:29 -0500
Subject: Sparbeize

Contents Retrieved from Microscopy Listserver Archives
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Charlie.

Sparbeize is indeed an additive to other etching solutions. It was
produced by a German company. (Max Hoeck o.H.G. in Duesseldorf).
It was used for etching mainly stainless steel samples with a high
Cr content. eg.
10 ccs HNO3
0.30 Vogels Sparbeize
100 ccs HCL
100 ccs dist H2O
Etching either at room temp or 50 degrees C.
As I can remember, the actual ingredients of Sparbeize were kept secret.

Cheers

Hans Brinkies
Professional Officer, Electron Microscopy and Metallography
Swinburne, University of Technology
School of Engineering and Science
Industrial Microscopy Laboratory
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au


From daemon Thu Aug 29 19:23:57 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Thu, 29 Aug 2002 17:15:36 -0700
Subject: Re: Occupational injury?

Contents Retrieved from Microscopy Listserver Archives
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Thanks to everybody who replied to my question. I started off answering each
reply individually, but there are just so many that I hope everybody will
accept this blanket gratitude. It seems to be a common problem among EM
people and other microscopists, so perhaps it could count as an occupational
injury. If it is, please everybody, take care to prevent it, as far as you
can.

Lesley Weston



From daemon Thu Aug 29 20:32:00 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 29 Aug 2002 18:25:08 -0700
Subject: Re: New SEM opproximate price

Contents Retrieved from Microscopy Listserver Archives
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Excellent!! what did you recommend for the purchase?

gg

At 04:07 AM 8/27/2002, you wrote:

} I appreciate the information. I think, I've got more than enough.
} Thank you all very much for comprehensive answers.
}
} Pavel



From daemon Fri Aug 30 02:33:03 2002



From: Gilles Hug :      gilles.hug-at-onera.fr
Date: Fri, 30 Aug 2002 12:20:41 +0200
Subject: Re: Probe analytical problem

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Hi,

pattex is a subdivision of Henkel / Germany. - I do not know if their
products are available outside of Germany ...
Take a look at their website http://www.pattex.de, probably the
"Kraftkleber" is the one you are looking for?

Greetings
Gunnar


} From: "Quinn, Tim Lee" {tquinn-at-ku.edu}
To: "'Microscopy-at-MSA.MIcroscopy.com'"
{Microscopy-at-sparc5.microscopy.com}



Hi,
You might want to check this paper:
3A critical evaluation of the results of the '92 round robin
microanalysis test(EDS & Peels) performed by the Ile de Frande
TEMnetwork, in MMM 4, p387-399. (multiple authors)
Analysis protocols were discussed in the instance of Olivine. If you
want a sample we usedyou should contact J. Ingrin (he is now somewher
in Toulouse).
Gilles

}
} Hallo Folks,
} Here's a baffling thing for you to mull over. I'm after some
} pointers after scratching my head for a couple of days. Here goes. We
} have a JEOL 733 with 4 WD spectrometers. We've the simple task of
} analysing some olivine crystals for their major (Si, Mg, Fe) and trace
} (Ca, Ni, Mn) elements. This should be a very simple, rudimentary and
} stressless thing to do, and normally is. The sad thing is, things aren't
} going normally. Standardisation is as simple as possible with as few
} standards as possible, and checked rigorously using materials for this
} purpose mounted in our standard block. However, when we go to the actual
} unknowns, Fe is sytematically about 3 wt % lower than expected for a
} stoichiometric olivine assuming the Mg and Si are ok. We can make this
} assumption because for an olivine of a specified SiO2 content we would
} expect a specified MgO content, and this checks out. For the given FeO
} content we would expect lower MgO and SiO2 contents, and this would
} simply make the totals worse. I've checked out the hardware and the fact
} that the calibration works for unknowns on the standard block suggests
} to me that there is no problem with the analytical protocol we're using.
} I considered C coating but thought that this is more likely to affect
} attenuation of the lower energy X-rays such as Si and Mg.
} Has anyone some suggestions on what the cause of this
} strange behaviour for a very simple mineral could be?
} Thanks,
} Malc.
}
} --
} Dr MP Roberts Phone: [+27](0)46 603 8313
} Dept of Geology Fax: [+27](0)46 622 9715
} Rhodes University Cell: 083 4060 262 (try your luck!)
} 6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
} SOUTH AFRICA


--
_______________________________________________________
Gilles Hug
LEM, UMR 104, ONERA-CNRS, BP72
92322 Chatillon Cedex, France
tel : +33 1 46 73 45 42 fax : +33 1 46 73 41 55
mailto:Gilles.Hug-at-onera.fr
http://www.onera.fr/lem/anachistr/index.html
_______________________________________________________
Office National d'Etudes et de Recherches Aerospatiales
------- & ------
Centre National de la Recherche Scientifique
_______________________________________________________


From daemon Fri Aug 30 07:04:53 2002



From: Ping Li :      pli-at-dal.ca
Date: Fri, 30 Aug 2002 08:55:59 -0300
Subject: Re: LF shielded computer monitor

Contents Retrieved from Microscopy Listserver Archives
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Could some of you please provide me some information regarding where to
purchase a 21" or larger low frequency shielded computer monitor? I would
like to buy one but having trouble to find a supplier. The monitor will be
used to replace the smaller one on our Tecnai TEM. Your help is greatly
appreciated.

Thank you,
Ping Li

--
Ping Li, Ph.D.
Director, Scientific Imaging Suite
Department of Biology
Dalhousie University
Halifax, NS B3H 4J1
Canada

Tel: 902-494-3309
Fax: 902-494-3736
E-mail: Ping.Li-at-Dal.Ca




From daemon Fri Aug 30 07:30:40 2002



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Fri, 30 Aug 2002 08:41:02 -0400
Subject: Mystery Resin

Contents Retrieved from Microscopy Listserver Archives
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I was not looking for purchase! Just the info


----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "ATC SEM Laboratory" {atcsem-at-earthlink.net}
Cc: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
Sent: Thursday, August 29, 2002 9:25 PM


Hi Everyone,

We got a brief protocol that listed a resin for TEM called
LX-112. Has anyone ever heard of it? Does anyone have a protocol?

Thanks!

Lesley



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Fri Aug 30 09:55:54 2002



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 30 Aug 2002 09:46:46 -0500 (CDT)
Subject: Re: Adhesive for binding plastic serial sections

Contents Retrieved from Microscopy Listserver Archives
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To adhere serial setions together into a ribbon while sectioning, I'v used
(with success) a small amount of a product called Tackiwax can be applied
to the sides of the blockface at the top and bottom of the trapezoid. I
think the reference is in M.A. Hayat's book on EM Techniques for
Biological Electron Microscopy.

I ordered a tub (1 lb. container - a lifetime supply for ultramicrotomy)
from Fisher Scientific, but other suppliers may be able to get it for you.
Here's information off of the label:

Boeker Tackiwax
Cat. No. 11444000
Boekel Scientific
855 Pennsylvania Blvd.
Feasterville, PA 19053

Heather Owen


Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall, P.O. Box 413
Milwaukee, WI 53210
USA

Phone: (414)229-6816



From daemon Fri Aug 30 10:33:48 2002



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Fri, 30 Aug 2002 11:22:42 -0400
Subject: Re: Mystery Resin

Contents Retrieved from Microscopy Listserver Archives
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Ladd makes it. It's very nice when using plastic culture dishes. I use the
same recipe as for epon 812. Let me know if you need specifics.
Mary Gail Engle

At 08:41 AM 8/30/02 -0400, Lesley S. Bechtold wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-9700


From daemon Fri Aug 30 11:03:07 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 30 Aug 2002 08:55:22 -0700
Subject: Re: LF shielded computer monitor

Contents Retrieved from Microscopy Listserver Archives
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The Sony Multiscan E500 (21") and most all of the Multiscan
series are shielded.

A better course might be to look into 20" flat panel LCDs.
I'm using a 20" RGB+sync unit made in Germany with
a NEC LCD display unit. It works quite well. NEC also
makes some 18" LCD displays that are very nice. I'm
not sure if they make larger ones. The 18" one I
have used is the 1850 and is the standard LCD display
on the FEI Sirion FEGSEM.

gary


At 04:55 AM 8/30/2002, you wrote:

} Could some of you please provide me some information regarding where to
} purchase a 21" or larger low frequency shielded computer monitor? I would
} like to buy one but having trouble to find a supplier. The monitor will be
} used to replace the smaller one on our Tecnai TEM. Your help is greatly
} appreciated.
}
} Thank you,
} Ping Li
}
} --
} Ping Li, Ph.D.
} Director, Scientific Imaging Suite
} Department of Biology
} Dalhousie University
} Halifax, NS B3H 4J1
} Canada
}
} Tel: 902-494-3309
} Fax: 902-494-3736
} E-mail: Ping.Li-at-Dal.Ca



From daemon Fri Aug 30 11:26:02 2002



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Fri, 30 Aug 2002 12:20:15 -0400
Subject: Re: Mystery Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Lesley,

LX-112 is the EPON replacement sold by LADD. We use is routinely and
follow the traditional Luft formulations.

Frank

At 08:41 AM 8/30/02 -0400, Lesley S. Bechtold wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Aug 30 13:14:34 2002



From: robert.fowler-at-tdktca.com
Date: Fri, 30 Aug 2002 14:10:05 -0400
Subject: salary survey

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi all
I am researching salaries. The problem I am having is my job is primarily
failure analysis, but I am a technician so most surveys list quality
technician and quality engineer. I have responsibilities in both areas. Any
help off or online would be most appreciated. 9 years experience.

Apologies for off topic discussion

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com


THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY
TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL
INFORMATION.

If you are not the intended recipient, be advised that any use,
dissemination, distribution or duplication of this transmission is strictly
prohibited. If you received this transmission in error, please notify the
sender immediately by electronic reply to this transmission or by phone
(847-803-6100). Thank you.




From daemon Fri Aug 30 15:42:26 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 30 Aug 2002 18:22:12 -0700
Subject: Re: New SEM opproximate price

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Lesley,

LX-112 is a Ladd Research product. It is used in wide variety of projects
from EM research to the aerospace industry.
Please reply directly with more details on your project, check our web site,
http://www.laddresearch.com, or call the number listed below and ask for Dr.
Charles Duvic our chemist and you can discuss it with him.

Thank you,

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Lesley S. Bechtold" {lsb-at-jax.org}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 30, 2002 8:41 AM


Ah....OK. Can you tell us what the basic premise was
for doing this? No condemnation, just wondering why
you are doing this exercise. As you can imagine,
there is a huge range of options, systems and prices.

I'm a cat....curious.

gary g.

At 05:22 AM 8/30/2002, you wrote:
} I was not looking for purchase! Just the info
}
}
} ----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
} To: "ATC SEM Laboratory" {atcsem-at-earthlink.net}
} Cc: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
} Sent: Thursday, August 29, 2002 9:25 PM
} Subject: Re: New SEM opproximate price
}
}
} } Excellent!! what did you recommend for the purchase?
} }
} } gg
} }
} } At 04:07 AM 8/27/2002, you wrote:
} }
} } } I appreciate the information. I think, I've got more than enough.
} } } Thank you all very much for comprehensive answers.
} } }
} } } Pavel
} }



From daemon Sat Aug 31 08:47:15 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 31 Aug 2002 09:35:50 -0500
Subject: Tackiwax vs. dental wax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Heather A. Owen wrote:
============================================================

To adhere serial setions together into a ribbon while sectioning, I'v used
(with success) a small amount of a product called Tackiwax can be applied to
the sides of the blockface at the top and bottom of the trapezoid. I think
the reference is in M.A. Hayat's book on EM Techniques for Biological
Electron Microscopy.

I ordered a tub (1 lb. container - a lifetime supply for ultramicrotomy)
from Fisher Scientific, but other suppliers may be able to get it for you.
Here's information off of the label:

Boeker Tackiwax
Cat. No. 11444000
Boekel Scientific
855 Pennsylvania Blvd.
Feasterville, PA 19053

==============================================================
Could someone explain how this product Tackiwax™ apparently a product of
Boekel Scientific would differ from a good dental wax, such as on URL
www.2spi.com/catalog/knives/cavex-set-up-dental-wax-sh.shtml

which is also used pretty widely in the world for ultramicrotomy
applications.

I am not suggesting that this common (but very high quality) dental wax is
better for this application, but it is far more readily available from a
large number of suppliers (such as one of our many competitors like Ted
Pella, Inc.). The Cavex® brand of dental wax, unlike a lot of the dental
wax products on the market, is actually approved for use in dentistry in
most countries around the world. What is not clear to me is whether soft,
medium or hard would be the preferred hardness for this particular
application (to adhere serial sections into a ribbon).

The cost to place a small order for a single item, for many customers, can
be staggering, so I ask this question because if it is not any different
than a good dental wax, such as the Cavex wax, it could keep people in
certain countries from placing a small order (because the shipping and other
costs of importation may be many times larger than the fob value of the item
itself). This is inherently a low cost item and as was pointed out
previously, one pack can last virtually a life time.

Disclaimer: SPI Supplies is a major supplier of high quality dental wax for
use in microtomy applications worldwide so naturally we would have a vested
interest in having customers order this wax from SPI along with their other
needed items.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sat Aug 31 19:50:48 2002



From: curari-at-asu.edu
Date: Sat, 31 Aug 2002 17:36:16 -0700 (MST)
Subject: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers

I am looking for a replacement part for our poloron sputter/coater. It
is a model 0E5000, manufacturing date unknown. The part that is broken is a
hollow glass cylinder that has a rubber seal at either end and acts as the
sputtering chamber. Any recommendations on a supplier or glass manufacturer is
welcomed or other ideas! Thank you in advance.

Wil Kunkel
curari-at-asu.edu
student of chemistry
This email was sent with 100.00% recycled electrons.


From daemon Sat Aug 31 22:56:59 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 31 Aug 2002 20:49:33 -0700
Subject: Re: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The Polaron (check spelling) supplier in the US should
be able to get this item for you. If not, that would be a
good reason to avoid this brand of coater.

Why did it fail? This seems to me to be a very low
failure rate item. The rubber boots do need to be
replaced at some interval (like on my Anatech Hummer
VII). But having the glass cylinder crack would be really
disturbing to me. Did you miss-treat it?

gary g.


At 05:36 PM 8/31/2002, you wrote:

} Dear Listers
}
} I am looking for a replacement part for our poloron
} sputter/coater. It
} is a model 0E5000, manufacturing date unknown. The part that is broken is a
} hollow glass cylinder that has a rubber seal at either end and acts as the
} sputtering chamber. Any recommendations on a supplier or glass
} manufacturer is
} welcomed or other ideas! Thank you in advance.
}
} Wil Kunkel
} curari-at-asu.edu
} student of chemistry
} This email was sent with 100.00% recycled electrons.



From daemon Sun Sep 1 04:53:12 2002



From: Zhang Qixing :      yt-zqx-at-sohu.com
Date: Sun, 1 Sep 2002 17:43:22 +0800
Subject: 12v dc saving energy light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

solar lamps
Please look at our web site:
{http://www.yt-economy.com/senke.htm}
Best regards
Zhang Qixing
address: nantong road no.71 Yantai China
Yantai senke co.ltd
tel:86-0535-6675831
fax:86-0535-6675830
post code:264000
email:yt-zqx-at-sohu.com


{ {---ÒÔÉÏÓʼþÄÚÈÝÓëÈí¼þ¿ª·¢ÉÌÎÞ¹Ø---} }
-----------------------------------------------
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ÏÂÔØµØÖ·: http://www.ehoosoft.com
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²¡¶¾Óʼþ¿ËÐÇ: ÒÚ»¢Email°²È«´óʦ
......


From daemon Sun Sep 1 04:53:13 2002



From: Zhang Qixing :      yt-zqx-at-sohu.com
Date: Sun, 1 Sep 2002 17:43:01 +0800
Subject: 12v dc saving energy light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


solar lamps
Please look at our web site:
{http://www.yt-economy.com/senke.htm}
Best regards
Zhang Qixing
address: nantong road no.71 Yantai China
Yantai senke co.ltd
tel:86-0535-6675831
fax:86-0535-6675830
post code:264000
email:yt-zqx-at-sohu.com


{ {---ÒÔÉÏÓʼþÄÚÈÝÓëÈí¼þ¿ª·¢ÉÌÎÞ¹Ø---} }
-----------------------------------------------
»¶Ó­Ê¹ÓÃÒÚ»¢EmailϵÁÐÈí¼þ
ÏÂÔØµØÖ·: http://www.ehoosoft.com
ÉÌÎñÓÊÏäËÑË÷: ÒÚ»¢EmailËÑË÷´óʦ
ÓʼþȺ·¢ÌØ¿ì: ÒÚ»¢EmailÓʲî
²¡¶¾Óʼþ¿ËÐÇ: ÒÚ»¢Email°²È«´óʦ
......



From daemon Sun Sep 1 04:53:14 2002



From: Zhang Qixing :      yt-zqx-at-sohu.com
Date: Sun, 1 Sep 2002 17:42:47 +0800
Subject: 12v dc saving energy light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


solar lamps
Please look at our web site:
{http://www.yt-economy.com/senke.htm}
Best regards
Zhang Qixing
address: nantong road no.71 Yantai China
Yantai senke co.ltd
tel:86-0535-6675831
fax:86-0535-6675830
post code:264000
email:yt-zqx-at-sohu.com


{ {---ÒÔÉÏÓʼþÄÚÈÝÓëÈí¼þ¿ª·¢ÉÌÎÞ¹Ø---} }
-----------------------------------------------
»¶Ó­Ê¹ÓÃÒÚ»¢EmailϵÁÐÈí¼þ
ÏÂÔØµØÖ·: http://www.ehoosoft.com
ÉÌÎñÓÊÏäËÑË÷: ÒÚ»¢EmailËÑË÷´óʦ
ÓʼþȺ·¢ÌØ¿ì: ÒÚ»¢EmailÓʲî
²¡¶¾Óʼþ¿ËÐÇ: ÒÚ»¢Email°²È«´óʦ
......



From daemon Sun Sep 1 11:03:54 2002



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Sun, 01 Sep 2002 10:53:47 -0500
Subject: Re: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Wil,

I recently had the same problem, and I went to the university's glass
shop in the chemistry department. Even though the size I needed was a
special order item, it still only cost $16, instead of the $120 the
sputter-coater company wanted.
Yours would cost more, since you would need a groove for the o-ring
ground into it, but you'd still save a lot of money.
If your department doesn't have a glass shop anyway (a lot of
university bean counters have closed the glass shops), then try the
big EM facility in mat sci and engineering (I forget the exact
department/institute, sorry, but someone on the list will know) --
they have a good-sized machine shop and might be able to make the
chamber.

Phil

} Dear Listers
}
} I am looking for a replacement part for our poloron sputter/coater. It
} is a model 0E5000, manufacturing date unknown. The part that is broken is a
} hollow glass cylinder that has a rubber seal at either end and acts as the
} sputtering chamber. Any recommendations on a supplier or glass
} manufacturer is
} welcomed or other ideas! Thank you in advance.
}
} Wil Kunkel
} curari-at-asu.edu
} student of chemistry
} This email was sent with 100.00% recycled electrons.

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Sun Sep 1 22:38:56 2002



From: curari-at-asu.edu
Date: Sun, 01 Sep 2002 20:24:02 -0700 (MST)
Subject: Re: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thank you everyone for the advice and help.

Wil

This email was sent with 100.00% recycled electrons.

On Sun, 1 Sep 2002, Philip Oshel wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Wil,
}
} I recently had the same problem, and I went to the university's glass
} shop in the chemistry department. Even though the size I needed was a
} special order item, it still only cost $16, instead of the $120 the
} sputter-coater company wanted.
} Yours would cost more, since you would need a groove for the o-ring
} ground into it, but you'd still save a lot of money.
} If your department doesn't have a glass shop anyway (a lot of
} university bean counters have closed the glass shops), then try the
} big EM facility in mat sci and engineering (I forget the exact
} department/institute, sorry, but someone on the list will know) --
} they have a good-sized machine shop and might be able to make the
} chamber.
}
} Phil
}
} } Dear Listers
} }
} } I am looking for a replacement part for our poloron sputter/coater. It
} } is a model 0E5000, manufacturing date unknown. The part that is broken is a
} } hollow glass cylinder that has a rubber seal at either end and acts as the
} } sputtering chamber. Any recommendations on a supplier or glass
} } manufacturer is
} } welcomed or other ideas! Thank you in advance.
} }
} } Wil Kunkel
} } curari-at-asu.edu
} } student of chemistry
} } This email was sent with 100.00% recycled electrons.
}
} --
} }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
} Philip Oshel
} Supervisor, AMFSC and BBPIC microscopy facilities
} Department of Animal Sciences
} University of Wisconsin
} 1675 Observatory Drive
} Madison, WI 53706 - 1284
} voice: (608) 263-4162
} fax: (608) 262-5157 (dept. fax)
}
}



From daemon Mon Sep 2 02:26:11 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 02 Sep 2002 00:17:30 -0700
Subject: Re: LF shielded computer monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Follow-up on shielded computer monitor.

These are the specs for my out of production E500:

http://www.ita.sel.sony.com/support/displays/legacy/specs/cpde500.pdf

I also use Sony Multiscan sfII shielded monitors on PCs and Macs.

Look at Emission/EMI and ELF/VLF specs for prospective units.

This is their new 21" monitor:
http://www.sonystyle.com/home/item.jsp?hierc=9683&itemid=8241

This is their 24" monitor (100 pounds--heavy!!):
http://www.sonystyle.com/home/item.jsp?hierc=9683&catid=9710&itemid=6971

These are shielded.

NEC makes good monitors too. Many of them are also shielded.
Check before you buy.

gary

At 04:55 AM 8/30/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Sep 2 17:12:32 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 2 Sep 2002 17:00:41 -0500
Subject: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


A faculty member needs to produce some extremely high resolution 24 x
35 mm negatives or masks to demonstrate the diffraction phenomenon
and FFT. He has generated around 24 PostScript files that consist of
a "unit cell" (such as an Escher figure) that is repeated numerous
times to mimic a crystalline lattice. The goal is to project a laser
through the negative masks and generate diffraction patterns as part
of the demonstration.

We have attempted to generate hi res 35 mm negs by means of a
LaserGraphics 8,000 line film recorder but the resolution is
inadequate. He needs the equivalent of 16,000 x 24,000 pixels in the
24 x 35 mm area. This is about 17,000 ppi!

Any suggestions? Any other practical ways to produce these masks?

I had considered micro-lithography but do not have access to the technology.

Many thanks,

John B.




##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Sep 3 03:11:31 2002



From: =?iso-8859-1?Q?Krzysztof_Jan_H=FCbner?= :      hubner-at-IOd.krakow.pl
Date: Tue, 3 Sep 2002 09:47:17 +0200
Subject: Fw: MIRAGE 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


John
Sounds too demanding for a demonstration.
Is it not possible to get the same value from the demonstration by
using a less complex image, with lower definition and fewer repeats?
Chris

----- Original Message -----
} From: "John J. Bozzola" {bozzola-at-siu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, September 02, 2002 11:00 PM





} } Dear colleagues and friends,
} }
} } please find below the ascii version of the first Call for Paper of the
}
} } conference MIRAGE'2003; please feel free to distribute it and to
} advertise.
} }
} } The website of the conference is http://telin.rug.ac.be/mirage2003
} }
} } Looking forward to meeting you in Rocquencourt,
} }
} } Jacques Blanc-Talon and Andre Gagalowicz
} }
} } } ---
} }
} } MIRAGE 2003
} }
} } Call for Papers
} }
} } Computer Vision / Computer Graphics
} } Collaboration for
} } Model-based Imaging,
} } Rendering,
} } image Analysis and
} } Graphical special Effects
} }
} } 10-12 March 2003
} } INRIA Rocquencourt, France
} }
} } MIRAGE 2003 is designed as a very peculiar conference in the field of
}
} } Information Technology: it is dedicated to computer vision and
}
} } computer graphics collaboration studied as a tool for the production
}
} } of mirages of the reality. In the domain of computer vision, this
}
} } collaboration may take the form of model-based approaches with
}
} } feedback, as models are essential to process, analyze, understand and
}
} } generate complex still and animated images in a stable manner. Such
}
} } models must be comprehensive and their parameters updated according to
}
} } the difference between the expectation (real image) and the
}
} } synthesized results. In the computer graphics domain, a special
}
} } emphasis will be set upon techniques using real images as input for
}
} } realistic rendering, visualization and special effects.
} }
} } MIRAGE 2003 is going to be an international conference where authors
}
} } are encouraged to submit papers on theoretical, computational,
}
} } experimental and industrial aspects of model-based image analysis and
}
} } synthesis. MIRAGE'2003 will be held in the new conference room of the
}
} } INRIA Rocquencourt research centre on March 10-12, 2003.
} }
} } Topics include (but are not limited to)
} }
} } * Model-based imaging and analysis
} } * Model-based vision approaches
} } * Model-based indexing and database retrieval
} } * Model-based object tracking in image sequences
} } * Image-based rendering and inverse rendering techniques
} } * New trends in model-based (and intrinsic) video compression
} techniques
} } * Virtual and augmented reality
} } * Generation of special effects
} } * Model-based evaluation of computer vision techniques, Image
} quality
} } * Applications:
} } o Human-computer interaction, haptics
} } o Virtual prototyping
} } o Post-production, computer animation
} } o Multimedia applications (including face and gesture
} recognition),
} } multimedia databases
} } o Military applications
} } o Medical and biomedical applications
} } o Applications of fractals and multifractals
} }
} } Venue
} }
} } The Symposium will take place within the INRIA Rocquencourt research
}
} } centre which can be easily reached by car, by train or by bus, either
}
} } by taking public transportations or the free INRIA facilities. The
}
} } different routes are fully detailed on the INRIA special webpage.
} }
} } Paper submission and review process
} }
} } Prospective authors should prepare a full paper and submit it
}
} } electronically. The paper should consist of 5-10 pages in A4 format
}
} } with 11 pt font and should conform to the style guidelines outlined on
}
} } the MIRAGE 2003 website. LaTeX style sheets, MSWord templates and more
}
} } detailed information on the submission process can be found on the
}
} } MIRAGE 2003 website.
} }
} } All submissions will be reviewed by at least 2 members of the Program
}
} } Committee; additional reviewers will be consulted if necessary. The
}
} } papers should provide sufficient background information and should
}
} } clearly indicate the original contribution. They should state and
}
} } discuss the main results and provide adequate references.
} }
} } Confererence proceedings
} }
} } Accepted papers will be published in the CD-Rom conference proceedings
}
} } in pdf-format. Authors will be encouraged to include additional
}
} } multimedia content like sound files, demo's and video sequences,
}
} } subject to space availability. Authors wishing to take advantage of
}
} } this possibility should contact the Steering Committee for additional
}
} } instructions.
} }
} } Important deadlines
} }
} } November 12, 2002 Full paper submission
} } December 20, 2002 Notification of acceptance
} } February 10, 2003 Camera-ready papers due
} } January 6, 2003 Early registration deadline
} } February 10, 2003 Late registration deadline
} } 10-12 March 2003 MIRAGE 2003
} }
} } Registration
} }
} } Registration will be available online at the beginning of
}
} } 2003. However, due to security policy, the number of seats is strictly
}
} } limited: thus, early registration is strongly recommended.
} }
} } Conference Chair
} }
} } Jacques Blanc-Talon, CTA, Arcueil, France
} } Andre Gagalowicz, INRIA, Rocquencourt, France
} }
} } Steering Committee
} }
} } Jacques Blanc-Talon, CTA, Arcueil, France
} } Andre Gagalowicz, INRIA Rocquencourt, France
} } Philippe Gérard, INRIA Rocquencourt, France
} } Wilfried Philips, Ghent University, Gent, Belgium
} } Dominique Potherat INRIA Rocquencourt, France
} }
} } Honorary Chair
} }
} } Hojjat Adeli, Ohio State University, Colombus, USA
} }
} } Program committee
} }
} } Ruzena Bajcsy, UC Berkeley, USA
} } Philippe Bolon, ESIA, Annecy, France
} } Mike Brooks, University of Adelaide, Australia
} } Knut Conradsen, IMM, Denmark Technical University, Denmark
} } Kostas Daniilidis, U-Penn, USA
} } Charles R. Dyer, University of Wisconsin-Madison, USA
} } Andrew Glassner, Coyote Wind, Seattle, USA
} } Cedric Guiard, Duran Duboi, France
} } Adrian Hilton, University of Surrey, UK
} } David Hogg, University of Leeds, UK
} } Xiaoyi Jiang, Technical University of Berlin, Germany
} } Denis Laurendeau, Laval University, Quebec City, Canada
} } Franz Leberl, Graz University of Technology, Austria
} } Ales Leonardis, Ljubljana University, Slovenia
} } Vittorio Murino, Verone University, Italy
} } Jean-Claude Paul, LORIA, France
} } Hichem Sahli, Vrije Universiteit Brussel, Belgium
} } Mubarak Shah, University of Central Florida, USA
} } Gilles Simon, LORIA, Vandoeuvre-les-Nancy, France
} } Franck Solina, Ljubljana University, Slovenia
} } Seah Hock Soon, NTU, Singapore
} } Geoff West, Curtin University, Australia
} } Geoff Wyvill, University of Otago, New Zealand
} } Luc Van Gool, ESAT, Leuven, Belgium (ETH, Zurich, Switzerland)
} }
} }
} --------------------------------------------------------------------------
--
}
}
}
}
} best regards!
}
} André Gagalowicz
}
}



From daemon Tue Sep 3 04:25:07 2002



From: dzemik-at-eko.lublin.pl
Date: Tue, 3 Sep 2002 11:16:33 +0100
Subject: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello all !
Maybe somebody knows how to analyse samples of clinoptilolite containing strontium on EDX detector. Strontium and Silicon have both peak in one line. Please help me. This is my doc work.




From daemon Tue Sep 3 10:22:36 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 3 Sep 2002 09:07:26 -0600
Subject: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


John,

I suppose you need the small periodicity to achieve larger "bragg angles" so
you can show the diffraction pattern. If you make your pattern bigger (fewer
dots per inch), your diffraction angles are reduced, but could you perhaps
make up for that by using a longer "camera length", i.e., project the
results on a screen further away, or use some optics? Perhaps you could use
an old slide projector in some inventive fashion.

By the way, what resolution is an 8000 line film recorder? 24 mm / 8000
lines = 3 microns per line or about 8000 dpi?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Monday, September 02, 2002 4:01 PM
To: Microscopy-at-sparc5.microscopy.com


A faculty member needs to produce some extremely high resolution 24 x
35 mm negatives or masks to demonstrate the diffraction phenomenon
and FFT. He has generated around 24 PostScript files that consist of
a "unit cell" (such as an Escher figure) that is repeated numerous
times to mimic a crystalline lattice. The goal is to project a laser
through the negative masks and generate diffraction patterns as part
of the demonstration.

We have attempted to generate hi res 35 mm negs by means of a
LaserGraphics 8,000 line film recorder but the resolution is
inadequate. He needs the equivalent of 16,000 x 24,000 pixels in the
24 x 35 mm area. This is about 17,000 ppi!

Any suggestions? Any other practical ways to produce these masks?

I had considered micro-lithography but do not have access to the technology.

Many thanks,

John B.




##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Sep 3 11:13:09 2002



From: Kevin Frischmann :      kfrisch-at-amnh.org
Date: Tue, 03 Sep 2002 12:05:28 -0400
Subject: Re: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


John,

A couple of details in your post raised some flags, though I'm not sure of the exact numbers below:

} He needs the equivalent of 16,000 x 24,000 pixels in the 24 x 35 mm area

- Though it's still a topic of debate, I believe the estimate for "pixel equivalent" of a fine-grained 35mm film shot with a high quality lens is around 20 million total pixels (~4000 dpi), which you've already exceeded by quite a bit with the 8,000 line LFR. Even if my memory on this issue is off by millions of pixels, I'm quite sure there is no way 35mm film could support 16,000 x 24,000 pixels.

As far as I can tell, you could get close by upgrading your Lasergraphics film recorder to their 16,000 line "Mark VI" model (16384 x 13448 addressable pixels), and use the 4" x 5" (~ 16k x 20k pixel equivalent film) camera back. You may want to see if some other manufacturer has a film recorder with more than 16,000 lines if you truly need 16,000 x 24,000 pixels.
We have the same 8,000 line LFR in our lab, so I can't give any insight on what the upgrade might cost.

I have no knowledge of or experience with producing masks, so I don't know if 4" x 5" is a viable option.

Kevin Frischmann
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: (212) 313-7975
Fax: (212) 496-3480
email: kfrisch-at-amnh.org
------------------------------------------------------------------------


At 05:00 PM 9/2/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Sep 3 13:02:01 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Tue, 3 Sep 2002 14:31:06 -0400
Subject: Re: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


John;

As a follow on, at 17,000 DPI, are you approaching (or passing) the
resolution of the 35mm film?

Does anybody know the size of the Silver Halide Molecules on the film?

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Tuesday, September 03, 2002 2:59 AM
To: John J. Bozzola
Cc: microscopy-at-sparc5.microscopy.com


Aperio's scanner will do this easily - see http://www.aperio.com

-----Original Message-----
} From: Kevin Frischmann [mailto:kfrisch-at-amnh.org]
Sent: Tuesday, September 03, 2002 12:05 PM
To: Microscopy-at-sparc5.microscopy.com


John,

A couple of details in your post raised some flags, though I'm not sure of
the exact numbers below:

} He needs the equivalent of 16,000 x 24,000 pixels in the 24 x 35 mm area

- Though it's still a topic of debate, I believe the estimate for "pixel
equivalent" of a fine-grained 35mm film shot with a high quality lens is
around 20 million total pixels (~4000 dpi), which you've already exceeded by
quite a bit with the 8,000 line LFR. Even if my memory on this issue is off
by millions of pixels, I'm quite sure there is no way 35mm film could
support 16,000 x 24,000 pixels.

As far as I can tell, you could get close by upgrading your Lasergraphics
film recorder to their 16,000 line "Mark VI" model (16384 x 13448
addressable pixels), and use the 4" x 5" (~ 16k x 20k pixel equivalent film)
camera back. You may want to see if some other manufacturer has a film
recorder with more than 16,000 lines if you truly need 16,000 x 24,000
pixels.
We have the same 8,000 line LFR in our lab, so I can't give any insight on
what the upgrade might cost.

I have no knowledge of or experience with producing masks, so I don't know
if 4" x 5" is a viable option.

Kevin Frischmann
Microscopy & Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Phone: (212) 313-7975
Fax: (212) 496-3480
email: kfrisch-at-amnh.org
------------------------------------------------------------------------


At 05:00 PM 9/2/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Tue Sep 3 13:41:32 2002



From: Lois Anderson :      landers-at-jhmi.edu
Date: Tue, 03 Sep 2002 14:35:24 -0400
Subject: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone ever trained an inexperienced EM tech and if so do you have
any suggested tactics?


From daemon Tue Sep 3 15:18:37 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 3 Sep 2002 15:09:49 -0500
Subject: Sorvall ultramicrotome available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Sorvall MT-2B Ultramicrotome for sale. Includes manual and accessories. If interested, contact me via e-mail.

Martin L. Katz, PH.D.
University of Missouri
Ophthalmology, Genetics, Pathobiology & Molecular Biology
Mason Eye Institute
One Hospital Drive
Columbia, MO 65212
(573) 882-8480
FAX (573) 884-4100
katzm-at-health.missouri.edu

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Tue Sep 3 15:35:29 2002



From: Giles, Bill :      William.Giles-at-timet.com
Date: Tue, 3 Sep 2002 14:28:44 -0600
Subject: RE: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Try looking at 14.164 Kev, should be a K line down there.

William Giles
Senior Electron Microscopist
Metallography Lab Coordinator
P.O. Box 2128
Henderson, Nevada 89009
(702) 566-4436
(702) 564-9038 (Fax)
Bill.Giles-at-timet.com

RE:
Hello all !
Maybe somebody knows how to analyse samples of clinoptilolite containing
strontium on EDX detector. Strontium and Silicon have both peak in one line.
Please help me. This is my doc work.



*


From daemon Tue Sep 3 16:53:58 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 03 Sep 2002 14:48:04 -0700
Subject: RE: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I missed on this one too. The fellow is looking for making a
slide, not scanning one. I think the highest resolution is probably
via a microcircuit stepper mask (5"x5" chrome on quartz).
The image size can be just about anything up to the size of
the plate. Price is big too.

gary

At 11:31 AM 9/3/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Sep 3 16:56:15 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 3 Sep 2002 17:56:10 -0400
Subject: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


John;

Photolithography, as is used in semiconductors, would certainly give you the
spatial resolution you desire but is an expensive proposition for a
demonstraion.

Peter Tomic
Anadigics, Inc.

-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Monday, September 02, 2002 6:01 PM
To: Microscopy-at-sparc5.microscopy.com


A faculty member needs to produce some extremely high resolution 24 x
35 mm negatives or masks to demonstrate the diffraction phenomenon
and FFT. He has generated around 24 PostScript files that consist of
a "unit cell" (such as an Escher figure) that is repeated numerous
times to mimic a crystalline lattice. The goal is to project a laser
through the negative masks and generate diffraction patterns as part
of the demonstration.

We have attempted to generate hi res 35 mm negs by means of a
LaserGraphics 8,000 line film recorder but the resolution is
inadequate. He needs the equivalent of 16,000 x 24,000 pixels in the
24 x 35 mm area. This is about 17,000 ppi!

Any suggestions? Any other practical ways to produce these masks?

I had considered micro-lithography but do not have access to the technology.

Many thanks,

John B.




##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Sep 3 17:05:21 2002



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 3 Sep 2002 15:42:21 -0700
Subject: Re: LF shielded computer monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


John,

there is no simple answer to your question, as the speed of the film has a
big influence on the resolution (faster films use larger grains). Also
development will change the number of pixels. I did a quick search on the
internet and I found 2 sites with specific answers. I have included excerpts
below, there is more information on those sites.

It seems, the answer is somewhere between 10 Million and 20 Million pixels,
but that is of course only a qualitative answer. A full answer would have to
take into account the modulation transfer function, dynamic range, etc. The
second link below goes into that a bit. For example, the author says that a
certain film can resolve 3500 line pairs (7000 pixels) over the width of the
film (35mm), but that only means, that at that resolution one can
distinguish a white line from a black line with some accuracy. In digital
parlance that would probably mean a dynamic range of 1 bit (black or white).
If you want to have a better dynamic range (to distinguish for example black
from dark gray), you would have to look at larger areas, thus reducing the
resolution.

mike



Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================


http://pic.templetons.com/brad/photo/pixels.html
How many pixels are there in a frame of 35mm film?

(an FAQ on digital photography)

This is a somewhat controversial question, and there are many possible
answers. Film is an analog medium, so it doesn't have "pixels" per se,
though film scanners have pixels and a specific resolution.

Today the one thing most people agree on is that it's a lot more than any
current consumer digital camera. The debate is about how much resolution the
digitals will have to reach to start matching the film.

The very short answer is that there are around 20 million "quality" pixels
in a top-quality 35mm shot. That's a shot with a tripod, mirror-up, with a
top-rate lens and the finest-grained film, in decent light. 12 million are
more typical for "good" shots. There may be as few as 4 million "quality"
pixels in a handheld shot with a point-and-shoot camera or camera with a
poor lens. And of course if focus is poor, or light is poor, or the camera
was not held steady, the number will drop down below the 1-2 million pixels
of the modern consumer digicam. Of course, one can have a bad shot with a
digital camera too, not using all its resolving ability. However, few pick
their gear with the plan of shooting badly.
..


http://www.bytesmiths.com/Services/scanning.html
The dimensions of a digital image are often confused with its resolution,
but it is actually a count of pixels (width by height) that is independent
of any unit of measure. A highest-quality, 35mm film scan will have
dimensions of about 3600x2400 pixels.
..





-----Original Message-----
} From: John W. Raffensperger, Jr. [mailto:johnr-at-helwigcp.com]
Sent: Tuesday, September 03, 2002 11:51 AM
To: microscopy-at-sparc5.microscopy.com


John;

As a follow on, at 17,000 DPI, are you approaching (or passing) the
resolution of the 35mm film?

Does anybody know the size of the Silver Halide Molecules on the film?

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Tuesday, September 03, 2002 2:59 AM
To: John J. Bozzola
Cc: microscopy-at-sparc5.microscopy.com


http://www.themonitoroutlet.com
has every monitor known to man.

John Mardinly
Intel

-----Original Message-----
} From: Ping Li [mailto:pli-at-dal.ca]
Sent: Friday, August 30, 2002 4:56 AM
To: Microscopy-at-sparc5.microscopy.com


Could some of you please provide me some information regarding where to
purchase a 21" or larger low frequency shielded computer monitor? I would
like to buy one but having trouble to find a supplier. The monitor will be
used to replace the smaller one on our Tecnai TEM. Your help is greatly
appreciated.

Thank you,
Ping Li

--
Ping Li, Ph.D.
Director, Scientific Imaging Suite
Department of Biology
Dalhousie University
Halifax, NS B3H 4J1
Canada

Tel: 902-494-3309
Fax: 902-494-3736
E-mail: Ping.Li-at-Dal.Ca




From daemon Tue Sep 3 20:36:05 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 4 Sep 2002 13:26:19 +1200
Subject: Re: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} Hello all !
} Maybe somebody knows how to analyse samples of clinoptilolite
} containing strontium on EDX detector. Strontium and Silicon have both
} peak in one line. Please help me. This is my doc work.
}
}
}

WDS would be better, but if you just can't get to it, maybe you could
bang the accelerating voltage up to 25 or even 30 kV and analyse
on the Sr Ka line at about 15 kV. I haven't done it for Sr, but I did
use 25 kV for Mo Ka to avoid SKa, worked OK.

good luck

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Sep 3 23:38:50 2002



From: Regina Himmelspach :      Himmelspach-at-rsbs.anu.edu.au
Date: Wed, 04 Sep 2002 14:27:17 +1000
Subject: TEM; need help on LR gold embedding in the Leica EM AFS freeze

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi all,

I would appreciate some advice on low temperature embedding in the Leica EM
AFS freeze substitution unit using LR gold. Sorry, since I am a beginner
(concerning TEM) some of my questions might sound a little odd.

My aim is to do immunogold labelling, mainly on cell walls, with sections
from Arabidopsis roots. The plan is to high pressure freeze Arabidopsis
roots, freeze substitute them, and embed them in LR gold at low temperature
(-25 degrees).

At the moment I am running a few tests before I start the real experiment.
And I am having trouble with the LR gold embedding.

This is what I tried so far:
I followed the protocol for "FS and low temperature embedding in
LEICA-capsules" from the AFS handbook (5.2, p.35). I was using LR gold +
0.5% Benzil (w/v) as embedding medium. I started polymerisation at -25
degrees with the UV lamp (Leica EM UV). After 24h the resin was not yet
solidified, only the bottom half of the capsules looked like it was solid.

My questions:
Does anybody have experience with low temperature embedding using LR gold
in the Leica EM AFS? Any ideas how long UV-polymerisation might take? (24h
was the information I got from the supplier for the LR gold). Or is there an
easier way of getting my specimens embedded, preferably in the Leica EM
AFS?

Thanks a lot for your help,

Regina



-------------------------------------------------------------------
Dr. Regina Himmelspach
Plant Cell Biology Group
Research School of Biological Sciences
The Australian National University
GPO Box 475
Canberra ACT 2601
Australia

ph: (+02) 6125-5561
fax: (+02) 6125-4331
himmelspach-at-rsbs.anu.edu.au




From daemon Tue Sep 3 23:42:29 2002



From: Allen Sampson :      ars-at-sem.com
Date: Tue, 3 Sep 2002 23:39:43 -0700
Subject: RE: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Interesting question, particularly now when EMs are found in such a wide
range of applications. I'll share what seems to work for me, although I
make no claim that this is the way training should be done.

I've trained quite a number of operators. I want them to first know those
things that can cause damage (i.e. - don't touch that TEM phosphor screen).
The instruments are frightening at first, and having some limits will
actually be comforting to them - they can focus on those things and relax
with the rest. Don't overload them with don't do's - just cover the things
that may cause expensive or time consuming damage.

My own desire is always to have the operator understand the physics behind
the instrument, and I always explain things in those terms. Knowing that
increasing the condenser current in an EM decreases the spot size gives you
little. Knowing that it reduces the chromatic spread or aberration of the
beam, and how, gives a broader understanding of the mechanism and the
result. While the distinction may not be terribly helpful in normal use it
gives them something that may help in the unusual circumstances.

That's what you really have to plan for in training, the unusual
circumstances. I've seen a number of customers in recent years who have
absolutely no knowledge of the instruments. 'Checklist operators' I call
them, and that's literally how they work. The person who bought the
instrument is long gone from the company, but he left a checklist for the
operation of the instrument that has been faithfully followed since. It's
a minor annoyance for me since I get service calls when someone
accidentally touches the wrong knob or they get a different type of sample
where the standard setup won't work. That stuff is minor, though, and
truly the extreme.

These are some really great tools, kind of like a hammer. When you need to
drive a nail, nothing works better. When you need to look at something
with a magnification greater than a light microscope, nothing beats EM.
But they are getting more trivialized as more production use is made of
them. These are more than a simple tool - they are the most sophisticated
equipment you'll find in a lab. Their proper and useful use requires
operators who know their limitations as well as their operation. One of
the things I always want to ensure is that the operators, if not the
management, understand what to really expect regarding the application and
interpretation of EMs.

All of this is in a lecture setting - I don't expect them to consciously
remember what I've told them over the hour or two it takes. Rather, I hope
that when they need it, they'll connect the dots with something I said and
figure out for themselves what's needed.

Then it's on to hands on training. This is generally very short - I can
teach someone to take a usable picture in five minutes. A little more time
looking over their shoulder while they look around. But then it's time to
get out.

The next two steps are the most important, bar none. Give them time to
play and learn for themselves the questions to ask, and follow up.

It takes time to get comfortable operating an instrument like these. It
takes time to play around with different samples and conditions. It takes
time to discover the questions you need help answering. The potential
operators simply have to spend a lot of time playing, not working, with an
EM to become good operators. If they are required to immediately start
working, you may get a good 'checklist operator' for the samples you
regularly run, and that may be enough. But if you want someone who really
understands the instrument, its use and its limitations, you have to
encourage them to explore the envelope.

Follow up - you have to follow up. Don't do it too quick, give them some
time to play. You have to take the initiative to query the potential
operator regarding the questions they have accumulated (suggest they write
them down) and the samples and conditions they have explored. This is your
chance to add details and refine their knowledge of the use and limitations
of the instrument. They're starting to learn the questions to ask - they
need to know that there is someone there to help with these problems. If
you're not there, then they will come to their own conclusions. Sometimes
they might be right, sometimes they might be wrong - in either case, if you
don't follow up you'll be missing out on your best possibility to really
train.

Have fun!


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Tuesday, September 03, 2002 11:35 AM, Lois Anderson
[SMTP:landers-at-jhmi.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Has anyone ever trained an inexperienced EM tech and if so do you have
} any suggested tactics?
}



From daemon Wed Sep 4 06:46:56 2002



From: Frank Thomas :      thomasf-at-gsca.NRCan.gc.ca
Date: Wed, 4 Sep 2002 08:32:37 -0300
Subject: LaB6 cathodes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Listers -

Does anyone know of any companies out there that rebuild LaB6 cathodes?
Has anyone had good/bad experiences with them?
Vendors, please reply off-list....

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada



From daemon Wed Sep 4 07:32:59 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 4 Sep 2002 08:31:17 -0400
Subject: RE: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The price is ~ $5K [U.S.] per photomask.

Peter

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Tuesday, September 03, 2002 5:48 PM
To: microbill-at-mohawk.net
Cc: MSA listserver


I missed on this one too. The fellow is looking for making a
slide, not scanning one. I think the highest resolution is probably
via a microcircuit stepper mask (5"x5" chrome on quartz).
The image size can be just about anything up to the size of
the plate. Price is big too.

gary

At 11:31 AM 9/3/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Sep 4 07:45:24 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 4 Sep 2002 08:44:07 -0400
Subject: RE: Strontium on Clinoptilolite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Bill;

It's true that Si & Sr overlap at ~ 1.8 KeV. The Sr major La1 line is at
1.806 KeV and the Si Ka1 major line is at 1.739 KeV. However, Sr is a much
more complex atom and has a Ka1 major line at 14.164 KeV as Bill Giles
mentioned below. Sr should be easily discernable from Si. Of course you
will have to have an incident electron energy of about 1.5 to 2X of the Sr
Ka1 energy to see it. I assume you can run your survey at 25 to 30 KeV?

Peter Tomic
Anadigics, Inc.



-----Original Message-----
} From: Giles, Bill [mailto:William.Giles-at-timet.com]
Sent: Tuesday, September 03, 2002 4:29 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Try looking at 14.164 Kev, should be a K line down there.

William Giles
Senior Electron Microscopist
Metallography Lab Coordinator
P.O. Box 2128
Henderson, Nevada 89009
(702) 566-4436
(702) 564-9038 (Fax)
Bill.Giles-at-timet.com

RE:
Hello all !
Maybe somebody knows how to analyse samples of clinoptilolite containing
strontium on EDX detector. Strontium and Silicon have both peak in one line.
Please help me. This is my doc work.



*


From daemon Wed Sep 4 07:51:48 2002



From: JHoffpa464-at-aol.com
Date: Wed, 04 Sep 2002 08:44:46 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


are you talking about training from scratch? ie fixation, sectioning etc? if so there is a long row to hoe. the best bet is always start from the somplest to the more complicated, and train in the order they would normaly do things, such as embedding then triming then sections (thicks then thins) staining then finaly to the scope.
always include the theory in with the practical. depending on how motivated the person is and how big an ego they have traing could take anywhere from a week to the person never getting it.
when i learned Em it was in a class room setting with the prof showing us once how to do things then for the rest of the semester we were on our own, lots of students droped the course. the few that stuck it out became very good and advanced techs. this is not i repeat not the recommended method of training someone. i still have thoughts of how to get even with the prof.
john


From daemon Wed Sep 4 08:57:07 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 4 Sep 2002 09:40:58 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


}
} Has anyone ever trained an inexperienced EM tech and if so do you have
} any suggested tactics?

***************
I have taught and EM course a number of times and have trained
technicians who had no background. Slow and steady wins the race. I
have found that taking the time to explain WHY things are done, and
WHAT the aims of each procedure are is very helpful. You can just
make people memorize techniques, but without understanding they will
always be automatons and won't be able to deal with anything
unexpected. So, don't just give them a dehydration protocol, explain
why you need to extract the water, etc. Also, take it one step at a
time, don't try to teach the person everything, tissue to grid, in
one fell swoop.

The good ting is that you can teach this person how to do things the
way you like, and they don't have any conflicting ideas.

Good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Sep 4 08:57:12 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Wed, 4 Sep 2002 10:22:25 -0400
Subject: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Allen touched on a significant point. I haven't had the pleasure of
working with an EM, but the concept applies to other technology as well.

As the "front ends" of technological devices get "better" and easier to
use, it becomes easier and easier to "get results". Just about anyone
can get some type of reading/image/measurement from a piece of modern
equipment.

Getting a result, and getting a MEANINGFUL result are two very different
things. Without a background understanding of the fundamental concepts
involved in how a particular device/technology works, the operator is at
risk of getting a result that may appear meaningful, but is beyond the
capabilities of the equipment (and/or sample preparation techniques).

An example I can directly relate to is on a 3-D Coordinate Measuring
Machine. In the "old days", the interfaces were crude, and you needed
to have a pretty thorough understanding of 3-D geometry just to get a
reading. Now, with the graphical interfaces, just about anyone can walk
up, use the joystick and touch the part in a few places, and get a
reading. But without the fundamental understanding, they may not have
set a meaningful reference plane and axis. As a result, they did indeed
get a result, but a meaningless one.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: Allen Sampson [mailto:ars-at-sem.com]
Sent: Wednesday, September 04, 2002 1:40 AM
To: 'Lois Anderson'; microscopy-at-sparc5.microscopy.com


Dear Wil,

We are the authorized Polaron dealer in the U.S. Please contact us at
ebs-at-ebsciences.com or by phone on (800) 992-9037 and we will be happy to
help you with any parts, service or new instrument requirements you have.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: "curari-at-asu.edu"-at-sparc5.microscopy.com
[mailto:"curari-at-asu.edu"-at-sparc5.microscopy.com]
Sent: Saturday, August 31, 2002 8:36 PM
To: Microscopy


Dear Listers

I am looking for a replacement part for our poloron sputter/coater.
It
is a model 0E5000, manufacturing date unknown. The part that is broken is a
hollow glass cylinder that has a rubber seal at either end and acts as the
sputtering chamber. Any recommendations on a supplier or glass manufacturer
is
welcomed or other ideas! Thank you in advance.

Wil Kunkel
curari-at-asu.edu
student of chemistry
This email was sent with 100.00% recycled electrons.




From daemon Wed Sep 4 09:55:34 2002



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Wed, 4 Sep 2002 10:47:56 -0400
Subject: Poloron Sputterer replacement parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Wil,

We are the authorized Polaron dealer in the U.S. Please contact us at
ebs-at-ebsciences.com or by phone on (800) 992-9037 and we will be happy to
help you with any parts, service or new instrument requirements you have.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: "curari-at-asu.edu"-at-sparc5.microscopy.com
[mailto:"curari-at-asu.edu"-at-sparc5.microscopy.com]
Sent: Saturday, August 31, 2002 8:36 PM
To: Microscopy


Dear Listers

I am looking for a replacement part for our poloron sputter/coater.
It
is a model 0E5000, manufacturing date unknown. The part that is broken is a
hollow glass cylinder that has a rubber seal at either end and acts as the
sputtering chamber. Any recommendations on a supplier or glass manufacturer
is
welcomed or other ideas! Thank you in advance.

Wil Kunkel
curari-at-asu.edu
student of chemistry
This email was sent with 100.00% recycled electrons.




From daemon Wed Sep 4 11:27:50 2002



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 4 Sep 2002 12:19:09 -0400
Subject: RE: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Lois,

We train 8-16 students how to use the SEM and TEM a year here. In
general 99% of them have no clue where to begin. I do follow pretty
much a line similar to what Allan Sampson talked about. BUT I don't go
deep into the physics of the system until they get out of the intro
class and into the advanced class. We have a 3 check out system. First
one is 2 hours with 2 students and me. Sample loading - what you can do
to screw it up - "review the checklist" - and generally go over the
basics - sample moving, mag, focus, collecting an image. The second
checkout is 2 hours with 1 student. I sit down and spend 15 minutes
going over everything with them and then leave them there with
instructions to collect 3-4 images of what ever they want (sample
already in). I check on them through the 2 hours - answer questions,
offer pointers, generally lightly supervise them. The final session is
basically instruction free. A test if you will. One so that they can
demonstrate to me that they understand enough about what they are doing
so as not to break anything and then they get keys to the rooms.
Instruction is carried out in the classes on specific areas with
demonstrations and lab write-ups. But even for student researchers that
are not enrolled in the class, they receive very similar instruction,
but tailored more to their individual project. The emphasis is always -
ask questions! Make sure they realize that if they are stuck then need
to ask questions rather than waste an hour or more of beam time trying
to figure something out themselves.

The standard checklist protocol is critical, but it can't be a
substitute for hands on instructions. Scare them but not enough that
they are afraid to touch stuff, reassure them so they have confidence
but not enough that they are more likely to break something. Emphasize
that if they DO do something resulting in a failure or a problem that
they document exactly what they did leading up to the problem and that
they won't get in trouble and that it is essential that what happened
leading up to the problem be fully disclosed so that the problem can be
fixed as fast as possible.

Hope this helps - it works for us here.

Geoff Williams
Microscopy Facility Supervisor

Checkout the new Biology Department Microscopy Facility web page.
Version 1 is now On-Line:
www.cst.cmich.edu/users/willi1gl/BDMF/BDMF.htm

} -----Original Message-----
} From: Lois Anderson [mailto:landers-at-jhmi.edu]
} Sent: Tuesday, September 03, 2002 2:35 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: trainee
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} Has anyone ever trained an inexperienced EM tech and if so do you have
} any suggested tactics?



From daemon Wed Sep 4 12:51:48 2002



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 4 Sep 2002 12:43:49 -0500
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


In training or help to train dozens of people over the years, I have run across one phenomenon that has always puzzled me. I first noticed this when showing people how to do specimen exchanges in TEM's in which a premature turning of the specimen arm before sufficient pumping on the airlock causes the high-voltage to shut down. Sometimes it would take around 20 minutes to get the scope back on line, and there can actually be introduction of a bit of oil into the column.

Needless to say, I wanted to warn trainees about this, so I first started telling them something like "Insert the specimen arm, BUT DO NOT TURN IT UNTIL....." Almost invariably, this resulted in exactly what I wanted to avoid, usually the first or second time I wasn't there to watch. Finally, I switched to saying "Insert the specimen arm and push it into place with the flat of your hand, then after the light goes out, turn it clockwise". That seems to work.

The point is, I guess, that in training people about things that can potentially damage equipment, I have found it best NOT to say "Don't ever do this!" It works much better (for me) to say "Do it like this." and demonstrate a way that minimizes the chance of an accident. Later on, after the habit has been established, I can explain the reasons without causing bad things to happen.

Has anyone else experienced this? Is this Edgar Allen Poe's "Imp of the Perverse" or just, gulp, me?

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




From daemon Wed Sep 4 12:51:48 2002



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Wed, 4 Sep 2002 13:49:20 -0400
Subject: M&M 2002 Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Is it too early to inquire as to the status of mailings of the proceedings?
If not, could someone direct me to the appropriate individual or committee
responsible for the mailings so that I could determine when I could expect
mine to arrive. I seem to remember that last year there were many postings
to the list on the subject of late deliveries. I haven't seen any this
year, so maybe I'm the exception. Please disregard this note if my copy is
already in my mailbox.





From daemon Wed Sep 4 14:28:11 2002



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Wed, 4 Sep 2002 15:22:10 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




Randy,
This is called Neuro-Linguistic Programming (NLP) by some.
http://www.nlptraining.com/index.asp

Disclaimer: The above link is for your information only.



} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} X-OriginalArrivalTime: 04 Sep 2002 17:43:49.0465 (UTC)
}
}
} In training or help to train dozens of people over the years, I have run
} across one phenomenon that has always puzzled me. I first noticed this
} when showing people how to do specimen exchanges in TEM's in which a
} premature turning of the specimen arm
} before sufficient pumping on the airlock causes the high-voltage to shut
} down. Sometimes it would take around 20 minutes to get the scope back on
} line, and there can actually be introduction of a bit of oil into the
} column.
}
} Needless to say, I wanted to warn trainees about this, so I first started
} telling them something like "Insert the specimen arm, BUT DO NOT TURN IT
} UNTIL....." Almost invariably, this resulted in exactly what I wanted to
} avoid, usually the first or second
} time I wasn't there to watch. Finally, I switched to saying "Insert the
} specimen arm and push it into place with the flat of your hand, then after
} the light goes out, turn it clockwise". That seems to work.
}
} The point is, I guess, that in training people about things that can
} potentially damage equipment, I have found it best NOT to say "Don't ever
} do this!" It works much better (for me) to say "Do it like this." and
} demonstrate a way that minimizes the
} chance of an accident. Later on, after the habit has been established, I
} can explain the reasons without causing bad things to happen.
}
} Has anyone else experienced this? Is this Edgar Allen Poe's "Imp of the
} Perverse" or just, gulp, me?
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
354 Mansfield Road
Beach Hall, Room 129
Storrs, CT 06269-2242

860 486-2914 voice
860 486-6369 fax
james.romanow-at-uconn.edu




From daemon Wed Sep 4 15:42:30 2002



From: Kim Rensing :      krensing-at-interchange.ubc.ca
Date: Wed, 04 Sep 2002 13:33:41 -0700
Subject: Re: TEM; need help on LR gold embedding in the Leica EM AFS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


} Hi all,
}
} I would appreciate some advice on low temperature embedding in the Leica EM
} AFS freeze substitution unit using LR gold. Sorry, since I am a beginner
} (concerning TEM) some of my questions might sound a little odd.
}
} My aim is to do immunogold labelling, mainly on cell walls, with sections
} from Arabidopsis roots. The plan is to high pressure freeze Arabidopsis
} roots, freeze substitute them, and embed them in LR gold at low temperature
} (-25 degrees).
}
} At the moment I am running a few tests before I start the real experiment.
} And I am having trouble with the LR gold embedding.
}
} This is what I tried so far:
} I followed the protocol for "FS and low temperature embedding in
} LEICA-capsules" from the AFS handbook (5.2, p.35). I was using LR gold +
} 0.5% Benzil (w/v) as embedding medium. I started polymerisation at -25
} degrees with the UV lamp (Leica EM UV). After 24h the resin was not yet
} solidified, only the bottom half of the capsules looked like it was solid.
}
} My questions:
} Does anybody have experience with low temperature embedding using LR gold
} in the Leica EM AFS? Any ideas how long UV-polymerisation might take? (24h
} was the information I got from the supplier for the LR gold). Or is there an
} easier way of getting my specimens embedded, preferably in the Leica EM
} AFS?
}
} Thanks a lot for your help,
}
} Regina

Regina,

LR Gold won't polymerize completely if there is oxygen present. The AFS is
supposed to exclude O2 by evaporating some of the LN2, but if there is a lot
of air movement around the machine (i.e., lots of people walking by, or in
front of an air vent) then it doesn't work so well. Make sure the inside
glass is in place during UV exposure.

If the bottom of the capsule is hard, you can pour off the top liquid, cut
off the goopy layer and use the hard bottom layer containing your specimen.

You should also make sure you are not introducing water (frost or
condensation) during processing. That will also prevent polymerization. You
may also retain some from the specimen if substitution is not complete.

Ask if you have more questions.

Good luck,

Kim

-------
Kim Rensing Ph.D.
Dept. of Botany, UBC
6270 University Blvd
Vancouver, BC, Canada
V6T 1Z4

phone: 604-822-5223
fax: 604-822-6089



From daemon Wed Sep 4 17:55:17 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 4 Sep 2002 18:43:06 -0400
Subject: RE: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


My procedure is to get out a box of razor blades and a bigger box of
Band-Aids. Then I demonstrate how to trim a block - bloodlessly. Then I do
it again, and again. The third trapezoid always has a base that is between
.5 and .75 mm. Then, I explain that I cut myself twice in the first week
and never again.

By the time I have finished and watched the first 10 minutes, I know whether
there is going to be a need for transfusions or not. If not, I walk away
for 20 minutes before I come back and inspect their work. I always make a
point to repair those attempts that are not 'up top snuff' and gush over
those that are. Then I make the gushers help the hindered until everyone is
on the same page.

One lab that way, usually the second, and sectioning always goes 50% better
than if I neglected to bring the Band-Aids. The first lab is always spent
cleaning the microscope.

Train 'em up good.

I had a sign on the outer back of the revolving darkroom door, which was
exposed whenever anyone entered and revolved it to all of those who looked
when they heard its noise. "Please don't enter the darkroom with the lights
on." And, I never fixed it or moved it. Somehow, after the first week, no
one ever left those light on either!

Hope you are well. I'm getting new scopes within next two months. New TEM
(FEI Technai 12T) and new ESEM (FEI Quanta 400) w/EDS. These days such
riches remind me of the poor man who won a Lincoln but had no money for gas.
There I'll sit for weeks after my Technai 12T is up and running, daintily
cutting grids from mesh with dull iris scissors. Then I'll have to go to
three Mile Island for uranium salts and to West Philadelphia for lead
tailings under old exterior window sills. And while I'm doing those
collections, I'll take a few hits by random gammas and then get mugged.
Microscopy is wonderful. Well, all won't be sad. I can analyze the skin on
my finger tips for contamination of Pb and U for no cost and under only
slight vacuum in my Quanta, and they won't dry out and I won't get
beam-burned.

The pre-installation costs are mounting to around $25,000 for three 208V (1
x 60 and 2 x 20A) supplies, removal of 30 ft. of base cabinetry, capping
plumbing of three sinks, running a dead 60A line for 15 ft, removing 1
fluorescent and 2 incandescent fixtures, and installing 50' of sound
proofing to reduce acoustic noise (of my screams!). We thankfully have
avoided the cost of jacking up our end of the building to determine the
cause of any vibrations, because there were none.

Cheers,

Fred

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/


} ----------
} From: Leona Cohen-Gould
} Sent: Wednesday, September 4, 2002 9:40 AM
} To: Lois Anderson; microscopy-at-sparc5.microscopy.com
} Subject: Re: trainee
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} }
} } Has anyone ever trained an inexperienced EM tech and if so do you have
} } any suggested tactics?
}
} ***************
} I have taught and EM course a number of times and have trained
} technicians who had no background. Slow and steady wins the race. I
} have found that taking the time to explain WHY things are done, and
} WHAT the aims of each procedure are is very helpful. You can just
} make people memorize techniques, but without understanding they will
} always be automatons and won't be able to deal with anything
} unexpected. So, don't just give them a dehydration protocol, explain
} why you need to extract the water, etc. Also, take it one step at a
} time, don't try to teach the person everything, tissue to grid, in
} one fell swoop.
}
} The good ting is that you can teach this person how to do things the
} way you like, and they don't have any conflicting ideas.
}
} Good luck,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}


From daemon Wed Sep 4 19:26:02 2002



From: Seth Ness :      seth.ness-at-mssm.edu
Date: Wed, 04 Sep 2002 20:04:58 -0400
Subject: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


hi,

I am isolating a rare monocyte population by FACS and trying to
look at it by TEM. on the first go around i isolated 8500 cells and
the pathologists were unable to get images of any cells becuase
the pellet was too small to see.

Are there any techniques to allow the imaging of very small
numbers of cells?


thanks,
---
Seth L. Ness M.D., Ph.D
Fellow in Human Genetics
Department of Human Genetics
Mount Sinai School of Medicine

Phone:212-241-6947
Fax:212-860-3316
sln8-at-columbia.edu

Ness Gadol Haya Sham



From daemon Wed Sep 4 19:59:44 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 04 Sep 2002 17:54:42 -0700
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


At 11:35 AM 9/3/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Flog them until they get with the program. However, this does
not adhere to ISO-9000 principles. But, who knows, perhaps
this could someday be included?

gg



From daemon Wed Sep 4 20:07:44 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 4 Sep 2002 21:06:35 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Jim;

You are not in the ether alone. I believe what happens is that sometimes
when an operator, or anyone for that matter, reads instructions, they
anticipate the next step in the procedure and sometimes they are wrong. We
have all done this in simple things like assembling toys or anything with
mundane instructions that seem intuitive when they aren't necessarily and
have several outcomes or degrees of freedom. I have been scolded on more
occasions than I want to recall by my wife for "NOT READING THE INSTRUCTIONS
ALL THE WAY FIRST!" Me being an engineer has yet to impress her in these
matters. She mentioned nothing about neuro-lingusitic programming whilst
yelling this at me. Needless to say I had to take the thing apart again.

Peter



-----Original Message-----
} From: Jim Romanow [mailto:bsgphy3-at-uconnvm.uconn.edu]
Sent: Wednesday, September 04, 2002 3:22 PM
To: microscopy-at-sparc5.microscopy.com




Randy,
This is called Neuro-Linguistic Programming (NLP) by some.
http://www.nlptraining.com/index.asp

Disclaimer: The above link is for your information only.



} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} X-OriginalArrivalTime: 04 Sep 2002 17:43:49.0465 (UTC)
}
}
} In training or help to train dozens of people over the years, I have run
} across one phenomenon that has always puzzled me. I first noticed this
} when showing people how to do specimen exchanges in TEM's in which a
} premature turning of the specimen arm
} before sufficient pumping on the airlock causes the high-voltage to shut
} down. Sometimes it would take around 20 minutes to get the scope back on
} line, and there can actually be introduction of a bit of oil into the
} column.
}
} Needless to say, I wanted to warn trainees about this, so I first started
} telling them something like "Insert the specimen arm, BUT DO NOT TURN IT
} UNTIL....." Almost invariably, this resulted in exactly what I wanted to
} avoid, usually the first or second
} time I wasn't there to watch. Finally, I switched to saying "Insert the
} specimen arm and push it into place with the flat of your hand, then after
} the light goes out, turn it clockwise". That seems to work.
}
} The point is, I guess, that in training people about things that can
} potentially damage equipment, I have found it best NOT to say "Don't ever
} do this!" It works much better (for me) to say "Do it like this." and
} demonstrate a way that minimizes the
} chance of an accident. Later on, after the habit has been established, I
} can explain the reasons without causing bad things to happen.
}
} Has anyone else experienced this? Is this Edgar Allen Poe's "Imp of the
} Perverse" or just, gulp, me?
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
354 Mansfield Road
Beach Hall, Room 129
Storrs, CT 06269-2242

860 486-2914 voice
860 486-6369 fax
james.romanow-at-uconn.edu




From daemon Thu Sep 5 02:17:04 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 5 Sep 2002 08:07:22 +0100
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
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} (FEI Technai 12T) and new ESEM (FEI Quanta 400) w/EDS. These days
such
} riches remind me of the poor man who won a Lincoln but had no money
for gas.
} There I'll sit for weeks after my Technai 12T is up and running,
daintily
} cutting grids from mesh with dull iris scissors. Then I'll have to
go to
} three Mile Island for uranium salts and to West Philadelphia for
lead
} tailings under old exterior window sills.

Luxury! We're so poor we have to shrink chicken wire in the washing
machine to make grids!
C.

} And while I'm doing those
} collections, I'll take a few hits by random gammas and then get
mugged.
} Microscopy is wonderful. Well, all won't be sad. I can analyze the
skin on
} my finger tips for contamination of Pb and U for no cost and under
only
} slight vacuum in my Quanta, and they won't dry out and I won't get
} beam-burned.
}
} The pre-installation costs are mounting to around $25,000 for three
208V (1
} x 60 and 2 x 20A) supplies, removal of 30 ft. of base cabinetry,
capping
} plumbing of three sinks, running a dead 60A line for 15 ft, removing
1
} fluorescent and 2 incandescent fixtures, and installing 50' of sound
} proofing to reduce acoustic noise (of my screams!). We thankfully
have
} avoided the cost of jacking up our end of the building to determine
the
} cause of any vibrations, because there were none.
}
} Cheers,
}
} Fred
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging
} Schmucker II Science Center
} c/o Geology/Astronomy
} West Chester University
} South Church Street and Rosedale Ave
} West Chester, Pennsylvania, USA, 19383
} Phone: 610-738-0437
} FAX: 610-738-0437
} fmonson-at-wcupa.edu
} CASI URL: http://darwin.wcupa.edu/casi/
} WCUPA URL: http://www.wcupa.edu/
} Visitors URL: http://www.wcupa.edu/_visitors/
}
}
} } ----------
} } From: Leona Cohen-Gould
} } Sent: Wednesday, September 4, 2002 9:40 AM
} } To: Lois Anderson; microscopy-at-sparc5.microscopy.com
} } Subject: Re: trainee
} }
}
} --------------------------------------------------------------------
----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
---.
} }
} }
} } }
} } } Has anyone ever trained an inexperienced EM tech and if so do you
have
} } } any suggested tactics?
} }
} } ***************
} } I have taught and EM course a number of times and have trained
} } technicians who had no background. Slow and steady wins the race.
I
} } have found that taking the time to explain WHY things are done,
and
} } WHAT the aims of each procedure are is very helpful. You can
just
} } make people memorize techniques, but without understanding they
will
} } always be automatons and won't be able to deal with anything
} } unexpected. So, don't just give them a dehydration protocol,
explain
} } why you need to extract the water, etc. Also, take it one step at
a
} } time, don't try to teach the person everything, tissue to grid,
in
} } one fell swoop.
} }
} } The good ting is that you can teach this person how to do things
the
} } way you like, and they don't have any conflicting ideas.
} }
} } Good luck,
} } Lee
} } --
} } Leona Cohen-Gould, M.S.
} } Sr. Staff Associate
} } Director, Electron Microscopy Core Facility
} } Manager, Optical Microscopy Core Facility
} } Joan & Sanford I. Weill Medical College
} } of Cornell University
} } voice (212)746-6146
} } fax (212)746-8175
} }
} }
}



From daemon Thu Sep 5 02:39:45 2002



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Thu, 05 Sep 2002 09:32:38 +0200
Subject: Long life-time fluorophore

Contents Retrieved from Microscopy Listserver Archives
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Hi listers,

First of all,
Sorry if some of you receive this email more than once, I'm sending this to
3 mailinglists at the same time...
I have a question about a fluorophore. I'm looking for a secondary
fluorescent antibody that has a really long life-time. The purpose is to
inject fixed cells (staining before fixation) into a mice so I can measure
the number of fluorescent cells in an organ after 60 days. I was thinking
about carboxyfluorescein, but does it really has a long fluorescent
life-time and/or are there any better secundary antibodies?
Thank you in advance,

Sven Terclavers



From daemon Thu Sep 5 08:04:21 2002



From: JHoffpa464-at-aol.com
Date: Thu, 05 Sep 2002 08:52:17 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
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floging could be part of you ISO-9000, if you include a proper porceedure.


From daemon Thu Sep 5 09:26:37 2002



From: Mike Peters :      petersmc-at-stanford.edu
Date: Thu, 05 Sep 2002 07:17:33 -0700
Subject: Re: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
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Greetings
With 8500 cells it sounds like the pathologists lost them while processing
the tissue (fixation, dehydration and infiltration). One way to circumvent
this is to "embed" the recovered cells in a pellet of albumin and
crosslinking the albumin with glutaraldehyde. Basically, you gently
centrifuge the cells of interest (after fixation and osmication) down
(6-800xG) in an eppendorf and resuspend in a tiny volume of buffer (say
20uL). Then, at the same time, add 50uL of 5% BSA and 50uL of 2%
glutaraldehyde. The glut fixes and cross links the BSA with the cells of
interest embedded within. Kind of like a cocoon. This is then spun down
and can then be transferred from the eppendorf, dehydrated and embedded in
EPON. It turns the 8500 cells into a tissue chunk. The BSA serves as a
"carrier" that impedes the steady loss of cells after each sequential
step. I was taught this technique back in the late 80's by a very well
respected microscopist by the name of Wille or Willy. Look for references
from UIC back in the 80's. Oh, the BSA doesn't interfere with images
because it is not osmicated. I plan on doing some EM on FACS samples later
this fall. Let me know what you decide to do or if I can be of further
assistance.

Mike



At 08:04 PM 9/4/2002 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Sep 5 09:36:14 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 05 Sep 2002 07:32:02 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
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At 04:39 AM 9/5/2002, you wrote:
} Hi XLrs,
}
} I frequently get "Unable to Allocate Memory" errors when trying to save
} images from MCTRL. Restarting Windows (NT) is the only way I have
} discovered will fix it. Anyone know what is going on?
} --
} ---
} Regards, Cameron.

This is highly suspicious of a defective app... not WinNT. Unlike
Win98, WinNT 4 & 5 handles memory totally differently, and very
well. If the app does a malloc() and does not release the memory
allocated, it is hung up. WinNT cannot perform garbage collection.
I would suggest reporting this to the vendor as an app bug.

gary g.



From daemon Thu Sep 5 09:36:14 2002



From: Materials Research Laboratories, Inc. :      mrllab-at-raex.com
Date: Thu, 05 Sep 2002 10:31:12 -0400
Subject: Re: Strontium on Clinoptilolite

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on 9/4/02 8:44 AM, Peter Tomic at PTomic-at-anadigics.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
}
A few additional comments - be sure to run a standard first for the Si.
Peak broadening would be expected at the lower Sr location (+ the Si), so if
you have a decent program, the separation can usually be done. As the others
mentioned, the higher energy lines will confirm the Sr - you did not mention
in your note if quant was desired; it sounds as though it is not necessary.
If just the presence of the Sr is of interest, use those heavier lines...as
Peter suggests, make sure the operating voltage is no lower than
25KeV..You'll need roughly double the energies to get your signal on these.
One other question..you say 'on' (the Si). Knowing the depth of the Sr is
important, too.

on 9/4/02 8:44 AM, Peter Tomic at PTomic-at-anadigics.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Bill;
}
} It's true that Si & Sr overlap at ~ 1.8 KeV. The Sr major La1 line is at
} 1.806 KeV and the Si Ka1 major line is at 1.739 KeV. However, Sr is a much
} more complex atom and has a Ka1 major line at 14.164 KeV as Bill Giles
} mentioned below. Sr should be easily discernable from Si. Of course you
} will have to have an incident electron energy of about 1.5 to 2X of the Sr
} Ka1 energy to see it. I assume you can run your survey at 25 to 30 KeV?
}
} Peter Tomic
} Anadigics, Inc.
}
}
}
} -----Original Message-----
} } From: Giles, Bill [mailto:William.Giles-at-timet.com]
} Sent: Tuesday, September 03, 2002 4:29 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE: Strontium on Clinoptilolite
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Try looking at 14.164 Kev, should be a K line down there.
}
} William Giles
} Senior Electron Microscopist
} Metallography Lab Coordinator
} P.O. Box 2128
} Henderson, Nevada 89009
} (702) 566-4436
} (702) 564-9038 (Fax)
} Bill.Giles-at-timet.com
}
} RE:
} Hello all !
} Maybe somebody knows how to analyse samples of clinoptilolite containing
} strontium on EDX detector. Strontium and Silicon have both peak in one line.
} Please help me. This is my doc work.
}
}
}
} *
}
}




From daemon Thu Sep 5 11:58:39 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 5 Sep 2002 11:49:27 -0500
Subject: RE: trainee

Contents Retrieved from Microscopy Listserver Archives
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Over the years I have taught a score of students to use an
ultramicrotome. I have evolved my approach so that I know start with
a trimmed block that is mounted and ready to cut. Once they get
sections, I back up the knife and start with the the simplest
alignment along the y axis, then i move on to x-axis and finally
z-axis alignment. when they get good at that, i switch to blocks
that need progressively more trimming. I have learned that the final
steps in microtomy are really the simplest if everything is perfect.
When students understand where they are going, it is easier for them
to learn the steps on how to get there. For example, it is hard for
them to know how much to trim off or why a small block face is better
if they have never seen a good block size or tried to thin section
one that is too big.


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu Sep 5 13:16:00 2002



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Thu, 5 Sep 2002 14:06:43 -0400
Subject: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
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Seth,
I have sectioned cell fractions by putting the sample into a clean
polypropylene eppendorf tube, centrifuge it well and do the total
fixation -} embedding without disturbing the "pellet". Care must be
taken to totally exchange the solutions. Repeat a step if there is a
question. After the resin (epon) is hard the next morning, pop it
out of the tube (I usually fill 1/4 of the tube with the final resin).
The pellet should be visible on the side of the cone at the bottom -
try using a dissecting microscope with the light under the the block.
It is usually necessary to cut the cone to a flat side and glue it
onto a blank so that it can be sectioned.
Pat

Patricia Stranen Connelly
The University of Pennsylvania
Department of Biology
Philadelphia, PA 91904-6018

215-898-7145
psconnel-at-sas.upenn.edu


From daemon Thu Sep 5 14:30:23 2002



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Thu, 5 Sep 2002 15:20:50 -0700
Subject: Re: imaging small samples

Contents Retrieved from Microscopy Listserver Archives
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Seth-
This is a small number for EM preparation. If I were you, I would
attach them to the bottom of one well in a 48-well plate using
poly-lysine to afix them. Then, you could embed them by making a
cast of the dish in epoxy resin. You need somebody reasonably adept
in the lab to do this, but it isn't hard.
Carol Heckman
(Bowling Green State University)



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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Sep 5 14:33:29 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 5 Sep 2002 15:32:19 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
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To be ISO compliant, one must have the proper documentation in place as well
as a gaggle of ISO type people to do the audits.

-----Original Message-----
} From: "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com
[mailto:"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com]
Sent: Thursday, September 05, 2002 8:52 AM
To: gary-at-gaugler.com; landers-at-jhmi.edu
Cc: Microscopy-at-sparc5.microscopy.com


floging could be part of you ISO-9000, if you include a proper porceedure.


From daemon Thu Sep 5 14:44:55 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 05 Sep 2002 12:40:24 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
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I'm working with a Sirion XL30 with Edax. MCTRL is running under
WinNT4 and is integrated with Soft Imaging analySIS. Host
computer is a Umax server. Never had a crash or memory
problem or had to reboot.

I still think it is a MCTRL version problem. And, any system
running Win 3.1 is an antique. If it is under contract, I would
have expected FEI to upgrade the system (they would have to
provide a new computer box to do this effectively since WinNT
is set up for the PCI cards. The 3.1 system is most certainly
ISA cards.) They probably don't want to put out the cost of
upgrading.

I upgraded my Amray SEM control from Win 3.1 to Win95 and
then to Win98SE. I used an ASUS motherboard with Pentium II
450MHz, 384MB RAM, dual 40G drives, Zip, floppy and
SCSI Jaz drive. The motherboard has one ISA slot which I
needed for the Nibblenet interface card. All of the other
cards are PCI. The Robinson BSE control program works fine
on any of the Win OS versions. Fortunately, it communicates
via serial port.

gary g.


At 11:48 AM 9/5/2002, you wrote:
} I had the same problem with my XL. FEI gave me no answers. I believe it
} has something to do with the EDAX integration. I got the error after heavy
} use of the EDAX software.
}
} Jon
}
} Gary Gaugler wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } At 04:39 AM 9/5/2002, you wrote:
} } } Hi XLrs,
} } }
} } } I frequently get "Unable to Allocate Memory" errors when trying to save
} } } images from MCTRL. Restarting Windows (NT) is the only way I have
} } } discovered will fix it. Anyone know what is going on?
} } } --
} } } ---
} } } Regards, Cameron.
} }
} } This is highly suspicious of a defective app... not WinNT. Unlike
} } Win98, WinNT 4 & 5 handles memory totally differently, and very
} } well. If the app does a malloc() and does not release the memory
} } allocated, it is hung up. WinNT cannot perform garbage collection.
} } I would suggest reporting this to the vendor as an app bug.
} }
} } gary g.



From daemon Thu Sep 5 16:46:01 2002



From: paul r hazelton, PhD :      paul_hazelton-at-umanitoba.ca
Date: Thu, 05 Sep 2002 16:37:06 -0500
Subject: Re: imaging small samples

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having had to visualize pellet sizes of below 1000 peripheral blood
monocytes in one earlier study of chlymidial infection, i can only say
that i had mixed success in looking for essentially non-visable
pellets. it may be a bit harsh to suggest that the pathologists simply
lost the cells during processing. even after embedding in either
agarose or albumin, the pellets, or embedded cores, will probably be
only marginally visable, at best.



From daemon Thu Sep 5 18:23:54 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 05 Sep 2002 16:17:30 -0700
Subject: Re: Memory allocation

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What is the computer system configuration? CPU, speed,
physical memory, motherboard, disk, etc?

gg

At 03:03 PM 9/5/2002, you wrote:
} Thought it might be appropriate to share our experience with the Memory
} Allocation Error............
}
} Our XL-40 was installed 3/97 as a stand alone 3.11 system. In 4/98 we
} completed conversion to NT 4.0 then in 9/98 embedded our EDS system. Very
} shortly thereafter (1 week), we began getting the dreaded Memory
} Allocation Error. The error occured with varying frequency, ranging from
} twice a day to twice a month. We found that a full PC shut down was
} required to clear the error. Over the years we tried many things to get
} rid of this error; to no avial. Following is a list of things we tried
} which did NOT appear to affect the frequency of the error:
}
} 1. save images directly to the C drive
} 2. save images directly to a network drive
} 3. added more memory
} 4. upgraded to XL v. 5.7
} 5. upgraded to service pack 4.0
} 6. changed out the PC (new Acer 11000)
} 7. changed out the hard drive
} 8. upgraded to service pack 5.0
} 9. upgraded to service pack 6a
} 10. installed multi user login shell (account log)
}
} We found experimentally that the error could be induced by alternately
} saving images and EDS spectra (about 20 each would usually do it). In
} early May of this year, we acquired a new EDS-WDS system, which was
} installed stand-alone (not embedded). We have not had a Memory Allocation
} Error since!
}
} For what it's worth....................
}
} Karen Dye
}
}
} } } } begg.4-at-osu.edu 09/05/02 04:39AM } } }
} Hi XLrs,
}
} I frequently get "Unable to Allocate Memory" errors when trying to
} save images from MCTRL. Restarting Windows (NT) is the only way I
} have discovered will fix it. Anyone know what is going on?
} --
} ---
} Regards, Cameron.



From daemon Thu Sep 5 19:43:33 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Thu, 05 Sep 2002 20:35:18 -0400
Subject: MicroZ-II XEDS detector

Contents Retrieved from Microscopy Listserver Archives
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Second try

} } Hi there guys,
} }
} } I have a Tracor Northern MicroZ-II XEDS detector sat in my lab that I
} } thought I might once be able to use. It has been kept cold since I
} } inherited it about 6 years ago and I finally have to admit that I am never
} } going to use it. So the question is, does anyone want it? It is a model
} } D-1830 with the serial number 02880935. It has a microscope flange attached
} } to it but I am unsure of the instrument that it came from.
} } "Buyer" collects or pays shipping and no whining about it not working, it is
} } as is. I haven't ever used it and so I have no idea if it works. The guy
} } who gave it to me assured me that it worked when it came off his SEM but as
} } I say that was 6 years ago.
} }
} } Any interest? Otherwise it goes to UM Property Disposition!
} }
} }
} } --
} } John Mansfield PhD MInstP
} } North Campus Electron Microbeam Analysis Laboratory
} } 417 SRB, University of Michigan
} } 2455 Hayward, Ann Arbor MI 48109-2143
} } Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} } (Leaving a phone message at 936-3352 is preferable to 834-3913)
} } Email: jfmjfm-at-engin.umich.edu
} } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} } Location: Lat. 42! 16' 48" Long. 83! 43' 48"
} }



From daemon Fri Sep 6 07:55:10 2002



From: JHoffpa464-at-aol.com
Date: Fri, 06 Sep 2002 08:43:41 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
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i love big block faces, 3mm wide are the best.


From daemon Fri Sep 6 07:55:21 2002



From: JHoffpa464-at-aol.com
Date: Fri, 06 Sep 2002 08:48:32 -0400
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
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you computer is over 5 years old, it's time to upgrade, by that i maean a whole new computer. with prices the way they are today for pcs it should be painless.


From daemon Fri Sep 6 10:49:14 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Fri, 06 Sep 2002 11:36:26 -0400
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
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That is all very well, but when you computer runs a microscope that is under
service contract and it is a microscope that is in use 20 hours per day,
then upgrading it is not just a question of going to Best Buy and getting
the latest e-Machines piece of you know what. FEI will not specify that
their system will run with the latest computers and you also need one with
an ISA slot and one that supports the video card they use to work with the
overlay card that exists in the ISA slot. (I am talking legacy equipment
here, the newer FEI systems use all PCI and have different configurations).
Most of you know that having a service contract on the instrument permits
you to have a gun change (a ten or so thousand dollar fix), if you have a
tip go bad in a field emission instrument (your $26,000 a year service
contract covers that). But it does not cover replacing the computer when it
becomes so slow in comparison with the other systems in the lab that you
think you are working on a Sinclair Spectrum!

On 9/6/02 8:48 AM, ""JHoffpa464-at-aol.com"-at-sparc5.microscopy.com"
{"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} you computer is over 5 years old, it's time to upgrade, by that i maean a
} whole new computer. with prices the way they are today for pcs it should be
} painless.
}
}

--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"




From daemon Fri Sep 6 10:49:19 2002



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 06 Sep 2002 10:05:35 -0500
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I hope you all understand that Microsoft isn't exactly telling the truth
when it pops up a memory allocation error. Okay, maybe it is telling the
truth, but the question is what truth?

Memory errors can pop up due to insufficient RAM on the computer (the kind
you can add more of), insufficient GDI or USER space (more about that
later), or even insufficient disk space (usually not a problem and easy to
correct). I have seen all three labeled as "memory" errors and usually
without a lot of hint as to which kind it was. The first and third issues
are fairly easy to verify. The second can be more of a challenge.

For the first, you can hit Ctrl-Alt-Delete under Windows NT or 2000 and
bring up the Task Manager to see how much memory is actually in use and how
much remains free. Windows 9x (and 3.x, I think) have a utility program
called SYSMON that can be installed to provide the same information. Both
types of Windows provide a swap file on disk to virtually extend your
memory beyond what can be held in the actual chips. It is slower to use the
swap file than real memory, but it should allow your system to keep running
even when it is allocating far more memory than is provided by the chips.

For the third, you should be able to open up a Windows Explorer window and
see how much free space you have left on your disk(s). Admittedly, this is
not the usual meaning of a memory error. (Disks are cheap now so there is
USUALLY an abundance of space. However, older Windows 3 machines might be
cramped for space.) However, I ran into one obscure program that needed
workspace on a hard drive and the drive that workspace was on was filling
up. The program itself, rather than Windows, coughed up the insufficient
memory problem. But Windows could generate such an error if disk space is
running low and Windows would like to extend the swap file but can't due to
a lack of space.

The second issue could be more troublesome to track down but even more of a
limitation. Windows allocates a small amount of memory, 64 kilobytes (not
megabytes), to various bookkeeping tasks. Under Windows 3, a total of 64 kB
was split between GDI (graphics stuff) and User (other various uses)
stacks. Microsoft realized that wasn't enough so they gave each their own
64 kB chunk under Windows 9.x - but that can still be rather stingy. They
also provided a tool, RSRCMTR.EXE, to track the use of that memory.
(Rsrcmtr SEEMS to show a third type of resource, System, but that is really
only the smaller of GDI and User.) Note that this small amount of memory is
set aside by Windows and it doesn't matter if your machine is loaded with
512 MB of SDRAM. You only get 64 KB each for GDI and User memory!!
(I would like to say something about Windows NT here, but I have recently
made the transition and cannot authoritatively say how they handle the
issue. It might be better.)

BTW, when GDI and/or User resources are depleted, any number of strange
symptoms can appear: icons can lose their appearance, icon names can
disappear or get corrupted, dialog boxes can turn illegible, etc.
Basically, you will notice.

As programs are loaded, GDI and User and regular memory will get consumed.
Some ill-written programs can allocate any or all of these three types of
memory and forget to free them up when they are done with them. This can
happen as the program runs continuously and documents are opened and
closed, or as the program is repeatedly started and stopped and restarted.
I have encountered many such programs over the years - even earlier
versions of Excel and Eudora were notorious for such behavior (chewing up
GDI and/or User memory). If it is a problem, it falls to the author to root
out the bug and fix it, and most authors do just that.

All that said, I don't know which flavor of memory problem you have.
Hopefully, you can determine that for yourself from my comments above and
pin down the problem. If you have further questions, feel free to ask.

Now I would like to hear from any NT gurus out there - what are the rules
for GDI and User space under NT and is there a good utility for tracking
their level? I have recently jumped to Windows 2000 and could find myself
facing the same issues as the original poster.

Warren

At 04:17 PM 9/5/02 -0700, you wrote:

} What is the computer system configuration? CPU, speed,
} physical memory, motherboard, disk, etc?
}
} gg
}
} At 03:03 PM 9/5/2002, you wrote:
} } Thought it might be appropriate to share our experience with the Memory
} } Allocation Error............
} }
} } Our XL-40 was installed 3/97 as a stand alone 3.11 system. In 4/98 we
} } completed conversion to NT 4.0 then in 9/98 embedded our EDS
} } system. Very shortly thereafter (1 week), we began getting the dreaded
} } Memory Allocation Error. The error occured with varying frequency,
} } ranging from twice a day to twice a month. We found that a full PC shut
} } down was required to clear the error. Over the years we tried many
} } things to get rid of this error; to no avial. Following is a list of
} } things we tried which did NOT appear to affect the frequency of the error:
} }
} } 1. save images directly to the C drive
} } 2. save images directly to a network drive
} } 3. added more memory
} } 4. upgraded to XL v. 5.7
} } 5. upgraded to service pack 4.0
} } 6. changed out the PC (new Acer 11000)
} } 7. changed out the hard drive
} } 8. upgraded to service pack 5.0
} } 9. upgraded to service pack 6a
} } 10. installed multi user login shell (account log)
} }
} } We found experimentally that the error could be induced by alternately
} } saving images and EDS spectra (about 20 each would usually do it). In
} } early May of this year, we acquired a new EDS-WDS system, which was
} } installed stand-alone (not embedded). We have not had a Memory
} } Allocation Error since!
} }
} } For what it's worth....................
} }
} } Karen Dye
} }
} }
} } } } } begg.4-at-osu.edu 09/05/02 04:39AM } } }
} } Hi XLrs,
} }
} } I frequently get "Unable to Allocate Memory" errors when trying to
} } save images from MCTRL. Restarting Windows (NT) is the only way I
} } have discovered will fix it. Anyone know what is going on?
} }
} } Regards, Cameron.

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Fri Sep 6 10:49:36 2002



From: Doug Keene :      drk-at-shcc.org
Date: Fri, 06 Sep 2002 08:37:44 -0700 (Pacific Daylight Time)
Subject: corrective eye surgery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists,

I hope to hear from anyone with good or bad personal
experience with lasik or other corrective eye surgery. I
am particularly anxious to know if the surgery adversely
affected vision through microscope binoculars, and the
difficulties with loosing near vision (I take my glasses
off for close work).

Many thanks,

Doug

----------------------
Douglas R. Keene
Micro-Imaging Center
Shriners Hospital for Children
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97239
Phone: 503-221-3434
FAX: 503-412-6894



From daemon Fri Sep 6 11:31:11 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Fri, 06 Sep 2002 09:23:49 -0700
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I always used to start by teaching them how to make glass knives. This
conveys the idea that we're dealing with very small things that need
enormous attention to detail. From there, I used to show them how to process
tissue, then how to trim a block and then move on to thick sections, thin
sections, staining, and finally using the microscope. The continuity of
following their own work from beginning to end made it more interesting, and
therefore memorable, for them. I also took care to explain why we needed to
do each individual action, and what would happen if we didn't. I gave each
one a copy of my lab manual, but I also insisted that they add their own
thoughts as we proceeded.
All this is in the past tense because I took early retirement as soon as
I could. This, along with endless patience and a sense of humour, is also
necessary.

Lesley Weston.



From daemon Fri Sep 6 11:39:02 2002



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Fri, 06 Sep 2002 09:29:44 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Well said, John. This is an issue that has been creeping up on us since
microscopes became intimately attached to computers. My 1983 Philips 410LS
runs pretty well with no RS232 and a stand-alone Gatan CCD. But my 1998
Hitachi SEM is a bit slow since it is tied to Windows 95. Hitachi informs
me that an upgrade to Windows NT 4.0 (that's right; not even Windows 2002
or XP) will cost close to $20K. We benefit from the versatility of a
computer interface but the manufacturer benefits from selling machines that
have the life span of a computer.

Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
http://katie.ucdavis.edu
raharris-at-ucdavis.edu


At 11:36 AM 9/6/2002 -0400, John F. Mansfield wrote:
} ----------------------------------------------------------.
}
}
} That is all very well, but when you computer runs a microscope that is under
} service contract and it is a microscope that is in use 20 hours per day,
} then upgrading it is not just a question of going to Best Buy and getting
} the latest e-Machines piece of you know what. FEI will not specify that
} their system will run with the latest computers and you also need one with
} an ISA slot and one that supports the video card they use to work with the
} overlay card that exists in the ISA slot. (I am talking legacy equipment
} here, the newer FEI systems use all PCI and have different configurations).
} Most of you know that having a service contract on the instrument permits
} you to have a gun change (a ten or so thousand dollar fix), if you have a
} tip go bad in a field emission instrument (your $26,000 a year service
} contract covers that). But it does not cover replacing the computer when it
} becomes so slow in comparison with the other systems in the lab that you
} think you are working on a Sinclair Spectrum!
}
} On 9/6/02 8:48 AM, ""JHoffpa464-at-aol.com"-at-sparc5.microscopy.com"
} {"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } you computer is over 5 years old, it's time to upgrade, by that i maean a
} } whole new computer. with prices the way they are today for pcs it should be
} } painless.
} }
} }
}
} --
} John Mansfield PhD MInstP
} North Campus Electron Microbeam Analysis Laboratory
} 417 SRB, University of Michigan
} 2455 Hayward, Ann Arbor MI 48109-2143
} Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} (Leaving a phone message at 936-3352 is preferable to 834-3913)
} Email: jfmjfm-at-engin.umich.edu
} URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} Location: Lat. 42! 16' 48" Long. 83! 43' 48"



From daemon Fri Sep 6 12:43:44 2002



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Fri, 6 Sep 2002 13:34:53 -0400
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


} Now I would like to hear from any NT gurus out there - what are the rules
} for GDI and User space under NT and is there a good utility for tracking
} their level? I have recently jumped to Windows 2000 and could find myself
} facing the same issues as the original poster.

Any good programmer should be able to identify a leaking program. Tracking
the leak back to the source code can be time consuming but is not
impossible. Checking for memory leaks is (or should be) part of any
software program testing procedure.


Here's a good starting place for Win32 programs.

http://msdn.microsoft.com/msdnmag/issues/01/03/leaks/leaks.asp

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Fri Sep 6 13:20:37 2002



From: Albert Coritz :      cactusgrower-at-earthlink.net
Date: Fri, 6 Sep 2002 14:12:56 -0400
Subject: RE: corrective eye surgery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Doug:

I had Lasix in February with spectacular results, it was worth every penny
I spent. I have 20/20 distance vision now plus good vision up close (8
inch WD) for fine work. I have some slight "Starburst" refraction at night
but it is does not effect my night vision. I am active in Astronomy too
with a large telescope at my disposal and notice no detrimental effects
from the surgery. As for the work at the Microscope or Microtome I think
it is much better than before I have the corrective surgery.

The most important thing is to choose a Lasix center that does the
procedure under "real" operating room conditions vs clean room environments
like some of the "Vi son Mills" in Malls have. I took my time and shopped
around not for the best price but the best center & surgeon.

--- Albert Coritz
--- cactusgrower-at-earthlink.net
Sales Manager EMS/ Diatome USA


} [Original Message]
} From: Doug Keene {drk-at-shcc.org}
} To: {Microscopy-at-sparc5.microscopy.com}
} Date: 9/6/2002 11:37:44 AM
} Subject: corrective eye surgery
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Microscopists,
}
} I hope to hear from anyone with good or bad personal
} experience with lasik or other corrective eye surgery. I
} am particularly anxious to know if the surgery adversely
} affected vision through microscope binoculars, and the
} difficulties with loosing near vision (I take my glasses
} off for close work).
}
} Many thanks,
}
} Doug
}
} ----------------------
} Douglas R. Keene
} Micro-Imaging Center
} Shriners Hospital for Children
} 3101 S.W. Sam Jackson Park Road
} Portland, Oregon 97239
} Phone: 503-221-3434
} FAX: 503-412-6894
}




From daemon Fri Sep 6 15:47:31 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 6 Sep 2002 15:37:41 -0500
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Scott,
That's fine if you are the software developer. I am a little
old academic using programs like MS word, netscape, photohop, acrobat
reader, and a few others on my computer. Nothing too wild. Well these
programs definitely leak memory. Once in a while I must restart the
computer after getting bogus memory errors. I suspect the Microsoft
products. But I havn't found any way for an ordinary user such as
myself to figure out *which* program is actually leaking.

Tobias

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Sep 6 21:57:04 2002



From: Alan Davis :      adavis-at-saipan.com
Date: Thu, 5 Sep 2002 20:48:49 +1000
Subject: photo strobe in microscope base?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I would be interested in hearing from anyone who has put a photographic strobe into a microscope stand, on the cheap, for better photos. Reflected light on Stereomicroscope is obvious; I am thinking of kohler illumination. My limited experiments, wven with a digital camera, have revealed exposure times to be limiting, not to mention vibration.

Alan Davis
Marianas High School,
Saipan

--
adavis-at-saipan.com 1-670-322-6580
Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI

I have steadily endeavored to keep my mind free, so as to give up any
hypothesis, however much beloved -- and I cannot resist forming one
on every subject -- as soon as facts are shown to be opposed to it.
-- Charles Darwin (1809-1882)

The right to search for truth implies also a duty; one must not
conceal any part of what one has recognized to be true.
-- Albert Einstein

As we enjoy great advantages from the inventions of others we should
be glad of an opportunity to serve others by any invention of
ours, and this we should do freely and generously.
-- Benjamin Franklin





From daemon Sat Sep 7 13:35:18 2002



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Sat, 07 Sep 2002 13:22:39 -0700
Subject: Re: Memory allocation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The problem of software compatibility between machines and operating system
versions is one which plagues not only users, but also manufacturers.

I began my career in this industry writing assembly code for the "minicomputers"
that were just starting to be tied to pulse-height analyzers in the early 1970s.
The challenge, of course, was to perform useful work in a 4K environment where not
even disk storage was available (and yes, I mean 4K, not 4M or 4G). When we added
peripherals, we had to write our own drivers -- and even mathematical algorithms
needed to be coded from scratch since computations generally had to be done in
"fixed point". The upside was that a programmer knew EXACTLY what was in the
computer and, after sufficient testing, could feel pretty confident about how the
code would perform under all plausible circumstances. Sure, we still introduced
our "bugs" -- and I introduced my share (as some members of this listserver can
personally testify) -- but we could figure them out by duplicating the
circumstances back in the factory. In extreme cases, you could take your debugging
right down to the individual machine instruction -- and frequently did.

Life has changed. Today, I'm sure that there is not a single human who fully
understands all of the intricacies of the Windows operating system (and some days I
think mankind in aggregate is clueless) -- not to mention all of the complexities
that occur with the addition of peripherals, utility software, and software
development tools such as compilers, linkers, library functions etc. -- look at a
typical desktop PC and there are probably thousands of people who have had a role
in establishing the integrity of the code that it is running. Sure, modern
operating systems impose barriers and structures such that programs run
independently, at least in principle, and IN PRINCIPLE, it should be possible to
freely switch between computers and add peripherals and capabilities without
incurring any problems. Some day that may be possible -- but that day hasn't yet
fully arrived.

The reality is that there remain many subtle ways in which programs can behave
properly in one environment, and not in another. With appropriate care in
programming practice, problems due to these things are rare, but when they do
occur, they are extremely difficult to isolate -- and costly, especially when they
need to be figured out in the customer's lab. Sometimes, the problem isn't even
the fault of the application programmer -- anyone who does this stuff (or manages
it) can tell you horror stories about undocumented bugs in compilers, operating
systems, and device drivers. The programmer's challenge in many cases is figuring
out how to get his/her stuff to run properly when the tools she/he must work with
aren't doing what they're supposed to.

Back in the days when instruments were "hard wired", they simply went obsolete in a
relatively short time (5 years wasn't unusual), and the user knew he/she had to
purchase a new one to get the latest/greatest features. As we have moved into a
"software" age, manufacturers have been able to provide software updates that add
features and prolong the life of the hardware. But as software environments have
grown more complicated and user expectations more demanding (and properly so),
manufacturers' software development and support costs have also grown
exponentially. Keep in mind that manufacturers of EM equipment don't enjoy the
luxury of large sales volumes, and it is easy to see that hiring a battery of
people to test software under every possible variant of computer, operating system,
and peripheral configuration just isn't feasible. So the only real practical
approach is to warrant system performance as it leaves the factory. Though most of
us would love to be nice guys and say -- "sure, just upgrade to a new computer when
you feel like it", users have a way of becoming testy and accusative when they
can't get the instrument to work properly with the new hardware (at least I am when
I find myself in this situation). One solution is to tell the user "upgrade at
your own risk" -- but this tends to generate ill will. The only realistic
alternative is for the manufacturer to take responsibility for the configuration --
and this is an expensive proposition.

So, though I appreciate and share the pain of having to buy expensive factory
upgrades (remember, we manufacturers buy stuff from other vendors and have similar
problems), I just want to point out that the presumption that the "manufacturer
benefits from selling machines that
have the life span of a computer" isn't the "gold mine" that it might appear to be
(ah -- if only that were the case!)

Fred Schamber
ASPEX LLC

Rick Harris wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Well said, John. This is an issue that has been creeping up on us since
} microscopes became intimately attached to computers. My 1983 Philips 410LS
} runs pretty well with no RS232 and a stand-alone Gatan CCD. But my 1998
} Hitachi SEM is a bit slow since it is tied to Windows 95. Hitachi informs
} me that an upgrade to Windows NT 4.0 (that's right; not even Windows 2002
} or XP) will cost close to $20K. We benefit from the versatility of a
} computer interface but the manufacturer benefits from selling machines that
} have the life span of a computer.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu
}
} At 11:36 AM 9/6/2002 -0400, John F. Mansfield wrote:
} } ----------------------------------------------------------.
} }
} }
} } That is all very well, but when you computer runs a microscope that is under
} } service contract and it is a microscope that is in use 20 hours per day,
} } then upgrading it is not just a question of going to Best Buy and getting
} } the latest e-Machines piece of you know what. FEI will not specify that
} } their system will run with the latest computers and you also need one with
} } an ISA slot and one that supports the video card they use to work with the
} } overlay card that exists in the ISA slot. (I am talking legacy equipment
} } here, the newer FEI systems use all PCI and have different configurations).
} } Most of you know that having a service contract on the instrument permits
} } you to have a gun change (a ten or so thousand dollar fix), if you have a
} } tip go bad in a field emission instrument (your $26,000 a year service
} } contract covers that). But it does not cover replacing the computer when it
} } becomes so slow in comparison with the other systems in the lab that you
} } think you are working on a Sinclair Spectrum!
} }
} } On 9/6/02 8:48 AM, ""JHoffpa464-at-aol.com"-at-sparc5.microscopy.com"
} } {"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com} wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } you computer is over 5 years old, it's time to upgrade, by that i maean a
} } } whole new computer. with prices the way they are today for pcs it should be
} } } painless.
} } }
} } }
} }
} } --
} } John Mansfield PhD MInstP
} } North Campus Electron Microbeam Analysis Laboratory
} } 417 SRB, University of Michigan
} } 2455 Hayward, Ann Arbor MI 48109-2143
} } Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
} } (Leaving a phone message at 936-3352 is preferable to 834-3913)
} } Email: jfmjfm-at-engin.umich.edu
} } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} } Location: Lat. 42! 16' 48" Long. 83! 43' 48"



From daemon Sat Sep 7 19:13:49 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Sat, 07 Sep 2002 17:02:03 -0400
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


on 9/4/02 1:43 PM, Tindall, Randy D. at TindallR-at-missouri.edu wrote:

} In training or help to train dozens of people over the years, I have run
} across one phenomenon that has always puzzled me. I first noticed this when
} showing people how to do specimen exchanges in TEM's in which a premature
} turning of the specimen arm before sufficient pumping on the airlock causes
} the high-voltage to shut down. Sometimes it would take around 20 minutes to
} get the scope back on line, and there can actually be introduction of a bit of
} oil into the column.
}
} Needless to say, I wanted to warn trainees about this, so I first started
} telling them something like "Insert the specimen arm, BUT DO NOT TURN IT
} UNTIL....." Almost invariably, this resulted in exactly what I wanted to
} avoid, usually the first or second time I wasn't there to watch. Finally, I
} switched to saying "Insert the specimen arm and push it into place with the
} flat of your hand, then after the light goes out, turn it clockwise". That
} seems to work.
}
} The point is, I guess, that in training people about things that can
} potentially damage equipment, I have found it best NOT to say "Don't ever do
} this!" It works much better (for me) to say "Do it like this." and
} demonstrate a way that minimizes the chance of an accident. Later on, after
} the habit has been established, I can explain the reasons without causing bad
} things to happen.
}
} Has anyone else experienced this? Is this Edgar Allen Poe's "Imp of the
} Perverse" or just, gulp, me?
}
Dear Randy,
Sue and I have a saying that the universe doesn't hear "not". I would
say, "Insert the specimen arm, then stop." I could then explain that one
has to wait for the vacuum to be established, etc. (In microscopes with a
vacuum-ready indicator, you could say, "...stop until the light goes on.")
On a humid day, you could explain that the electrons need a vacuum on the
inside of the column, and the trainee needs the air on the outside, so the
pumpdown is necessary to go from outside to inside, during the time you are
waiting to introduce the next step.
Yours,
Bill Tivol



From daemon Sun Sep 8 12:00:24 2002



From: David Knecht :      knecht-at-uconn.edu
Date: Sun, 8 Sep 2002 12:51:09 -0400
Subject: sticky shutter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


We have a shutter that has become sticky opening and closing. Does
anyone have any suggestions concerning an appropriate lubricant to
apply to the leaves to try and smooth its motion. Thanks- Dave



From daemon Sun Sep 8 16:06:18 2002



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Sun, 8 Sep 2002 19:13:47 -0400 (EDT)
Subject: Re: sticky shutter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Oils should not be used to lubricate leaf shutters or iris diaphragms.
Paradoxically, instead of acting as a lubricant the surface tension of
the oil film
will bind the leaves together and prevent them from sliding freely.
Problems with sticking are likely caused by dust or a particle of
grit,
a buildup of crud somewhere, excessive wear or mechanical damage.
Careful cleaning of the shutter may be a better bet.
Chris

----- Original Message -----
} From: "David Knecht" {knecht-at-uconn.edu}
To: "microscopy" {microscopy-at-sparc5.microscopy.com}
Sent: Sunday, September 08, 2002 5:51 PM


If it is a Vincent Assoc. Uniblitz shutter, don;t apply anything to it.
Call them.

___________________________________
WORK: http://www.aecom.yu.edu/aif/


On Sun, 8 Sep 2002, David Knecht wrote:
} We have a shutter that has become sticky opening and closing. Does
} anyone have any suggestions concerning an appropriate lubricant to
} apply to the leaves to try and smooth its motion. Thanks- Dave
}
}
}



From daemon Mon Sep 9 01:42:04 2002



From: Stacey Andringa :      Stacey.Andringa-at-uc.edu
Date: Mon, 09 Sep 2002 08:17:58 -0400
Subject: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dave,

Contact Miller-Stephenson Chemical Company at (203)743-4447 or
(818)896-4714. Ask
for the MS-143V or MS-145 for brush, not the aerosol. They provide free
samples. You may specify the amount of dry lubricant in the fluid. 1% to 5%
is all you need.

Both these products are used as dry lubricants and mold release agents. Both
are solids suspended in volatile fluid. High vacuum compatible. I use those
on JEOL TEMs mechanical shutter blades (pretty large blades) under the
viewing screen, as well as on other mechanisms.

Make sure that the shutter blades are squeaky clean, before applying these
products. No dust, oil, fibers, etc.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile

This message is made of 100% recycled electrons.
----- Original Message -----
} From: David Knecht {knecht-at-uconn.edu}
To: microscopy {microscopy-at-sparc5.microscopy.com}
Sent: Sunday, September 08, 2002 12:51 PM


Does anyone have a method to de-plasticize sections in Spurr resin that
can allow immunohist to be done on them?
Thanks.
Stacey Andringa



From daemon Mon Sep 9 08:27:35 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Mon, 9 Sep 2002 08:18:45 -0500
Subject: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


My name is Terry Ellis I work for Hallmark Cards Inc. and I run their
SEM-EDX equipment and also use a metallurgical, stereo and regular
microscope for sample prep and various projects. I am a diabetic and have
had several eye problems and surgeries.
one, the Doctor used a laser several to tack down my retina this has
reduced my night vision some but hasn't affected my microscope use.
Two, I had a detached retina in one eye then a year later a blood
vessel burst in the other eye, both times the doctor used microsurgery to
remove the fluid in my eye and lasers to correct the problems. Thank
goodness I still can see fine with both eyes, I have had to use a hat and
dark glasses since the body fluid that replaced the old fluid gel is much
clearer and I am more sensitive to light, on the microscopes I just use
less light.
Three, I had to have the lenses in both eyes replaced due to
cataracts in them, one lenses for distance and the other lens for close-up,
I have set up the binocular microscopes for my eyes by setting one eye
piece in focus then setting the other eye piece, which has an separate
focus knob, in focus then I can use the microscopes focus knobs to bring
it all in focus, which means that no one else can use them but I have a
camera and monitor that I move and set up for each microscope when some one
else needs to see what I see.
Four, I developed glaucoma in one eye and another Doctor put in what
he called a valve in it to relieve the pressure. I have lost some vision in
that eye but have been able to adjust the binoculars so I can almost see as
good with the bad eye but I generally use just one eye since I tend to get
headaches when I use both a lot.
During the surgery's I could only see with one eye and my boss still
calls me the one eyed microscopist.
Terry Ellis
tellis2-at-hallmark.com
816-545-6573



From daemon Mon Sep 9 11:06:29 2002



From: rcmoretz-at-att.net
Date: Mon, 09 Sep 2002 15:56:36 +0000
Subject: Re: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Stacey:
The deplasticization techniques using sodium methoxide
(do a quick search at Pubmed, or check some of the
classic biological TEM books for the exact recipes) is
the only way I know to remove any of the epoxies from
samples. Whether or not you are going to have any
antigen expression left can only be determined by doing
the experiment.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
Ridgefield, CT
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone have a method to de-plasticize sections in Spurr resin that
} can allow immunohist to be done on them?
} Thanks.
} Stacey Andringa
}
}


From daemon Mon Sep 9 16:14:50 2002



From: Steve Barlow :      sbarlow-at-sciences.sdsu.edu
Date: Mon, 9 Sep 2002 13:56:00 -0700
Subject: re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have started taping myself with a digital video camera running
through a procedure such as critical point drying or preparing
formvar coated grids. I transfer this session to my Mac and use
iMovie to edit the session, then burn it to a CD-ROM. My students
are given this custom-made CD before meeting me in the lab. Since
they have now seen the procedure demonstrated in my lab using my
protocols, I quiz them on the details of the procedure, then dismiss
those who fail the quiz and have them return at a later date to quiz
again. Those who pass are then required to demonstrate the
procedure. I can then give them individualized help on anything they
have a problem with during the procedure. Upon successfully and
safely completing the procedure, the user is considered 'checked out'
on the procedure and able to use that procedure or equipment
unsupervised.

The CD enables users to review a given procedure. It is often
difficult for new trainees to make detailed enough notes that capture
all the nuances. With the CD, however, they can easily back up and
review complex sections. If they have been off cloning for the last 4
months and forgotten all the steps of a procedure, they have the
CD-ROM to review.

This approach has cut down on my demo-as-training time and is
changing how I teach my TEM protocols lab. The time I used to spend
doing demo-training is shifting more toward protocol check-out. My
first attempts using these customized CD-ROMS were enthusiastically
encouraged by my SEM students last semester.

Steve


--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/


From daemon Mon Sep 9 20:29:39 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 9 Sep 2002 21:25:46 -0400
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I hope this is not some notion of a joke. It's a bad subject to joke about.
This all sounds highly improbable. I doubt whether Ray Charles would have
this many issues with vision.

PT

-----Original Message-----
} From: Terry E Ellis [mailto:tellis2-at-hallmark.com]
Sent: Monday, September 09, 2002 9:19 AM
To: microscopy-at-sparc5.microscopy.com


My name is Terry Ellis I work for Hallmark Cards Inc. and I run their
SEM-EDX equipment and also use a metallurgical, stereo and regular
microscope for sample prep and various projects. I am a diabetic and have
had several eye problems and surgeries.
one, the Doctor used a laser several to tack down my retina this has
reduced my night vision some but hasn't affected my microscope use.
Two, I had a detached retina in one eye then a year later a blood
vessel burst in the other eye, both times the doctor used microsurgery to
remove the fluid in my eye and lasers to correct the problems. Thank
goodness I still can see fine with both eyes, I have had to use a hat and
dark glasses since the body fluid that replaced the old fluid gel is much
clearer and I am more sensitive to light, on the microscopes I just use
less light.
Three, I had to have the lenses in both eyes replaced due to
cataracts in them, one lenses for distance and the other lens for close-up,
I have set up the binocular microscopes for my eyes by setting one eye
piece in focus then setting the other eye piece, which has an separate
focus knob, in focus then I can use the microscopes focus knobs to bring
it all in focus, which means that no one else can use them but I have a
camera and monitor that I move and set up for each microscope when some one
else needs to see what I see.
Four, I developed glaucoma in one eye and another Doctor put in what
he called a valve in it to relieve the pressure. I have lost some vision in
that eye but have been able to adjust the binoculars so I can almost see as
good with the bad eye but I generally use just one eye since I tend to get
headaches when I use both a lot.
During the surgery's I could only see with one eye and my boss still
calls me the one eyed microscopist.
Terry Ellis
tellis2-at-hallmark.com
816-545-6573



From daemon Mon Sep 9 22:17:08 2002



From: David Knecht :      knecht-at-uconn.edu
Date: Mon, 9 Sep 2002 23:10:24 -0400
Subject: Microscope table

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


We are being asked for information about tables to put a microscope
system on in a new lab being built. I wonder if anyone has suggestions
on what to look for in a microscope table or specific brands if known.
We are not planning an air table for this system. I should note that
when Leica spec'd our new SP2 confocal, they claimed we did not need an
air table, and so far they have been proven right. Hopefully the new
building will be as stable. Right now, the microscope in question
(Zeiss axio 200) is on a heavy duty generic metal table, on which we
have placed a heavy stone benchtop slab supported by antivibration pads
(audiophile variety). That has worked OK so far, but any suggestions
given the chance to purchase something new are welcome. Thanks- Dave



From daemon Tue Sep 10 03:49:36 2002



From: moritz-andreas.meyer-at-amd.com
Date: Tue, 10 Sep 2002 10:39:54 +0200
Subject: =?iso-8859-1?Q?glass_or_ceramics_rod_for_400=B0C_experiment_in?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi there,

here is what I am after. We have a self-made heating stage for an SEM, that can go up to 400°C. For thermal insulation the heater is mounted on a glass rod. When trying to mount everything I have broken the rod &-[.

Does anyone know where I can order glass or ceramics rods (or any oder material tha can take 400°C). Our former supplier is not available anymore.
I need the following size: diameter 10 mm and lenght 120 mm.
Is there a supplier in germany ?

Thanks for the help.

Andreas M.


Moritz Andreas Meyer
Dipl.-Ing. (FH), MSc.
Materials Analyst
Materials Analysis Laboratory

AMD Saxony Limited Liability Company & Co. KG
Wilschdorfer Landstraße 101
M/S E23-MA
D-01109 Dresden
F. R. Germany

Tel. +49-351-277-4149
Fax +49-351-277-9-4149
moritz-andreas.meyer-at-amd.com





From daemon Tue Sep 10 07:07:24 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 10 Sep 2002 07:56:22 -0400
Subject: RE: Microscope table

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Morning David,

We have an Olympus FV-300 confocal system for which we initially
used tables a la carte. The arrangement took up much space and left parts
of the system inaccessible. We have been forced to move the confocal system
to a smaller room which cannot accommodate the three tables on which we had
the system spread. Ten feet of space has dictated the table/rack
configuration and selection. The table on which we are locating the
microscope is sized to take the anti-vibration slab on which the microscope
and detector/confocal box are located. Then a second table for the computer
boxes and monitor and a third for the laser rack and power supplies. I
worked directly with the primary supplier (NOT the company from which we are
buying!) to insure that the design was consistent with our needs. The
circuitous purchase route was required by the fact that the primary supplier
expected payment on delivery (from an educational establishment?) whereas
the microscope supplier knew better.
My point is that for us the space to which we moved the system
dictated the support system and its distribution.
Finally, our three tables/racks ARE being custom made and were
purchased ($2,000) through the company that supplied the confocal system
itself. Something similar can be seen on the back page of the Fluoview
brochure which can be downloaded as PDF from:

http://www.olympusamerica.com/seg_section/seg_product.asp?p=&sc=&product=133
&fl=10

Beyond the issues enumerated, I agree, that nothing special is required. I
ended up with hand-drawn plans for the pieces which I attached to the quote
as specifications.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

THINKING IS MUCH MORE DIFFICULT THAN MEMORIZING.


} ----------
} From: David Knecht
} Sent: Monday, September 9, 2002 11:10 PM
} To: microscopy
} Subject: Microscope table
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We are being asked for information about tables to put a microscope
} system on in a new lab being built. I wonder if anyone has suggestions
} on what to look for in a microscope table or specific brands if known.
} We are not planning an air table for this system. I should note that
} when Leica spec'd our new SP2 confocal, they claimed we did not need an
} air table, and so far they have been proven right. Hopefully the new
} building will be as stable. Right now, the microscope in question
} (Zeiss axio 200) is on a heavy duty generic metal table, on which we
} have placed a heavy stone benchtop slab supported by antivibration pads
} (audiophile variety). That has worked OK so far, but any suggestions
} given the chance to purchase something new are welcome. Thanks- Dave
}
}
}


From daemon Tue Sep 10 07:39:45 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Tue, 10 Sep 2002 07:32:00 -0500
Subject: really no joke

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Peter:
No its all true, I wish it wasn't but it is true. I have been a type
1 Diabetic since 1963. Check out Diabetes and its long term complications.
I didn't mention it but I have also been diagnosed with the start of renal
kidney failure. I am on a vegetarian diet to slow down the failure rate and
with a couple of pills it has slowed down. I also didn't mention a bad
sprain that I did about 5 months ago that didn't heal and stayed swollen
and the foot doctor said this has caused the calcium to be leached out of
my foot so he has had me on crutches for the past two months until the foot
heals and the bones in my foot recalcifly.
I posted this in reply to a question about lasic surgery and meant
to encourage the questioner to do what he needed to do. Really the fact
that I work with microscopes has been a plus since I have been able to make
up for my eye problems and still do my job to my bosses satisfaction. With
a lot of other jobs I would have been out of work.
No Joke
Terry Ellis



From daemon Tue Sep 10 08:39:20 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Tue, 10 Sep 2002 09:25:12 -0400
Subject: Re: Microscope table

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


David,
We have several Vibraplane air tables (nitrogen) by Kinetic Systems
which we are very
happy with.
Mike D.

David Knecht wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are being asked for information about tables to put a microscope
} system on in a new lab being built. I wonder if anyone has suggestions
} on what to look for in a microscope table or specific brands if known.
} We are not planning an air table for this system. I should note that
} when Leica spec'd our new SP2 confocal, they claimed we did not need an
} air table, and so far they have been proven right. Hopefully the new
} building will be as stable. Right now, the microscope in question
} (Zeiss axio 200) is on a heavy duty generic metal table, on which we
} have placed a heavy stone benchtop slab supported by antivibration pads
} (audiophile variety). That has worked OK so far, but any suggestions
} given the chance to purchase something new are welcome. Thanks- Dave



From daemon Tue Sep 10 08:52:34 2002



From: Anaspec Luc Harmsen :      luc-at-anaspec.co.za
Date: Tue, 10 Sep 2002 15:43:28 +0200
Subject: Thanks to all who attended ICEM15

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi all
I would like to thank, on behalf of the ICEM15 committee, all those attended
the ICEM 15 conference held in Durban, South Africa last week.
We realised that getting people to come down South to South Africa would be
a first for most, but we were overwhelmed with the numbers that did attend.
It also caught the manufacturers, who hosted brilliant social functions, a
little off guard too

We have started to change the website to accommodate the "after party
pictures" and have copies of the programme etc available to you.
Please let us know if we can assist in any way.

Again thanks to all who attended and see you all in Japan in 2006.

P.S. Nestor, do you think that the photo of you and me at the meet and greet
function could be worth money?

Luc Harmsen
Marketing ICEM15
www.icem15.com






From daemon Tue Sep 10 09:42:22 2002



From: Ron L'Herault :      lherault-at-bu.edu
Date: Tue, 10 Sep 2002 10:32:32 -0400
Subject: One eyed micorocopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


} }
} } I have only one functioning eye (since birth) and was asked by the
} } researcher who wanted to hire me if it would be a problem running the
} } scanning microscope. I told him I could watch TV for hours without a
} } problem. The screen of the scope was not much different so I was and
am a
} } successful SEM operator. I can't evaluate stereo images or make use of
a
} } stereo microscope but I can still use them.
} }
} } Ron
} } ----- Original Message -----
} } } From: "Terry E Ellis" {tellis2-at-hallmark.com}
} } To: {microscopy-at-sparc5.microscopy.com}
} } Sent: Tuesday, September 10, 2002 8:32 AM
} } Subject: really no joke
} }
} }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Peter:
} } } No its all true, I wish it wasn't but it is true. I have been a
type
} } } 1 Diabetic since 1963. Check out Diabetes and its long term
complications.
} } } I didn't mention it but I have also been diagnosed with the start of
renal
} } } kidney failure. I am on a vegetarian diet to slow down the failure
rate
} } and
} } } with a couple of pills it has slowed down. I also didn't mention a
bad
} } } sprain that I did about 5 months ago that didn't heal and stayed
swollen
} } } and the foot doctor said this has caused the calcium to be leached out
of
} } } my foot so he has had me on crutches for the past two months until the
} } foot
} } } heals and the bones in my foot recalcifly.
} } } I posted this in reply to a question about lasic surgery and
meant
} } } to encourage the questioner to do what he needed to do. Really the
fact
} } } that I work with microscopes has been a plus since I have been able to
} } make
} } } up for my eye problems and still do my job to my bosses satisfaction.
With
} } } a lot of other jobs I would have been out of work.
} } } No Joke
} } } Terry Ellis
} } }
} } }
} } }
} }
}
}



From daemon Tue Sep 10 09:42:27 2002



From: msteglic-at-mail.mdanderson.org
Date: Tue, 10 Sep 2002 09:37:39 -0500
Subject: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




I have had good results doing immuno on Epon-Araldite embedded tissue by etching
the plastic with sodium metaperiodate using the method described in the article
"Ultrastructural Localization of Antigenic Sites on Osmium-fixed Tissues
Applying the Protein A-Gold Technique" by Bendayan and Zollinger as published in
The Journal of Histochemistry and Cytochemistry, Vol 31:1, pp101-109, 1983.




Stacey Andringa {Stacey.Andringa-at-uc.edu} on 09/09/2002 07:17:58 AM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Mannie Steglich/MDACC)


Does anyone have a method to de-plasticize sections in Spurr resin that
can allow immunohist to be done on them?
Thanks.
Stacey Andringa








From daemon Tue Sep 10 11:48:26 2002



From: Bill Miller :      microbill-at-mohawk.net
Date: Tue, 10 Sep 2002 12:40:55 -0400
Subject: Re: One eyed micorocopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The HIROX HiScope makes 3-D discernable on a flat screen with only one eye
by utilizing kineopsis instead of stereopsis. see
http://www.hirox-usa.com

Bill Miller

At 10:32 AM 9/10/2002 -0400, Ron L'Herault wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Sep 10 12:22:27 2002



From: joe fu :      jofu-at-nist.gov
Date: Tue, 10 Sep 2002 13:15:30 -0400
Subject: SEM give away

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...
After 20 some years calibrating SRM484,our VG HB-50A UHV SEM come to the
end. We are planning to dismantle the this cold FE, UHV microscope. Does
any University or non-profit organization want this baby (still function)
for free? Please contact me.

Joseph Fu
National Institute of Standards & Technology
100 Bureau drive Stop 8212
Gaithersburg, MD. 20899-8212
Tel: 301-975-3495
Fax: 301-869-0822
Email: jofu-at-nist.gov




From daemon Tue Sep 10 13:36:07 2002



From: msteglic-at-mail.mdanderson.org
Date: Tue, 10 Sep 2002 13:29:20 -0500
Subject: de-plasticizing Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html






________________________

I do not use Spur's so I don't know if this will work but I have had good
results doing immuno on Epon-Araldite embedded tissue by etching the plastic
with sodium metaperiodate using the method described in the article
"Ultrastructural Localization of Antigenic Sites on Osmium-fixed Tissues
Applying the Protein A-Gold Technique" by Bendayan and Zollinger as published in
The Journal of Histochemistry and Cytochemistry, Vol 31:1, pp101-109, 1983.



Stacey Andringa {Stacey.Andringa-at-uc.edu} on 09/09/2002 07:17:58 AM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Mannie Steglich/MDACC)


Does anyone have a method to de-plasticize sections in Spurr resin that
can allow immunohist to be done on them?
Thanks.
Stacey Andringa










Stacey Andringa {Stacey.Andringa-at-uc.edu} on 09/09/2002 07:17:58 AM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Mannie Steglich/MDACC)


Does anyone have a method to de-plasticize sections in Spurr resin that
can allow immunohist to be done on them?
Thanks.
Stacey Andringa








From daemon Tue Sep 10 14:27:34 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 10 Sep 2002 15:26:10 -0400
Subject: really no joke

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


My apologies to you and everyone else I may have offended. I truly did not
mean to do that and thought someone was making light of a serious medical
condition.

You must tell me what Hallmark needs an SEM for. I'm just awfully curious
as to its' application in that business.

Peter

-----Original Message-----
} From: Terry E Ellis [mailto:tellis2-at-hallmark.com]
Sent: Tuesday, September 10, 2002 8:32 AM
To: microscopy-at-sparc5.microscopy.com


Peter:
No its all true, I wish it wasn't but it is true. I have been a type
1 Diabetic since 1963. Check out Diabetes and its long term complications.
I didn't mention it but I have also been diagnosed with the start of renal
kidney failure. I am on a vegetarian diet to slow down the failure rate and
with a couple of pills it has slowed down. I also didn't mention a bad
sprain that I did about 5 months ago that didn't heal and stayed swollen
and the foot doctor said this has caused the calcium to be leached out of
my foot so he has had me on crutches for the past two months until the foot
heals and the bones in my foot recalcifly.
I posted this in reply to a question about lasic surgery and meant
to encourage the questioner to do what he needed to do. Really the fact
that I work with microscopes has been a plus since I have been able to make
up for my eye problems and still do my job to my bosses satisfaction. With
a lot of other jobs I would have been out of work.
No Joke
Terry Ellis



From daemon Tue Sep 10 17:46:14 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 10 Sep 2002 15:35:02 -0700
Subject: Re: Microscope table

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


TMC makes nice floating granite stable tables using
the same principle as for their SEM stabilizing platforms.

These babies are HEAVY. The one I've used is about
6" thick....solid granite. Floats like a feather. Has an
Olympus MX-50 confocal on it.

gary g.


At 08:10 PM 9/9/2002, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Sep 10 20:44:49 2002



From: Damian Neuberger :      neuberger1234-at-attbi.com
Date: Tue, 10 Sep 2002 20:35:17 -0500
Subject: Re:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Peter,

I doubt that it is a joke as it sound entirely plausable. I have some
friends who deal with numerous life threatening health problem on a daily
basis, fortunately I'm not one of them. I'm not making light of Terry
Ellis's eye issues but some of these people make this the short list of what
can go wrong. I'm not sure the human body was built on sound engineering
principles; sometimes it's a miracle that it works as well as it does for as
long as it does.

Damian Neuberger
double lung transplant recipient
}
} I hope this is not some notion of a joke. It's a bad subject to joke
about.
} This all sounds highly improbable. I doubt whether Ray Charles would have
} this many issues with vision.
}
} PT
}
} My name is Terry Ellis I work for Hallmark Cards Inc. and I run
their
} SEM-EDX equipment and also use a metallurgical, stereo and regular
} microscope for sample prep and various projects. I am a diabetic and have
} had several eye problems and surgeries.
} one, the Doctor used a laser several to tack down my retina this has
} reduced my night vision some but hasn't affected my microscope use.
} Two, I had a detached retina in one eye then a year later a blood
} vessel burst in the other eye, both times the doctor used microsurgery to
} remove the fluid in my eye and lasers to correct the problems. Thank
} goodness I still can see fine with both eyes, I have had to use a hat and
} dark glasses since the body fluid that replaced the old fluid gel is much
} clearer and I am more sensitive to light, on the microscopes I just use
} less light.
} Three, I had to have the lenses in both eyes replaced due to
} cataracts in them, one lenses for distance and the other lens for
close-up,
} I have set up the binocular microscopes for my eyes by setting one eye
} piece in focus then setting the other eye piece, which has an separate
} focus knob, in focus then I can use the microscopes focus knobs to bring
} it all in focus, which means that no one else can use them but I have a
} camera and monitor that I move and set up for each microscope when some
one
} else needs to see what I see.
} Four, I developed glaucoma in one eye and another Doctor put in
what
} he called a valve in it to relieve the pressure. I have lost some vision
in
} that eye but have been able to adjust the binoculars so I can almost see
as
} good with the bad eye but I generally use just one eye since I tend to get
} headaches when I use both a lot.
} During the surgery's I could only see with one eye and my boss still
} calls me the one eyed microscopist.
} Terry Ellis
} tellis2-at-hallmark.com
} 816-545-6573
}
}
}




From daemon Wed Sep 11 05:46:58 2002



From: EasyDownline :      besthomebiz-at-subdimension.com
Date: Wed, 11 Sep 2002 06:51:24 -0500
Subject: 1,500+ paid members in my downline in 30 days 9/11/02 6:51:24 AM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


This Program put over 1500 paid members in my downline
in 30 days without much of my own efforts.

It's the most effective recruiting system for the internet.
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We belong to the same opt-in list. But if you wish to have your email
address REMOVE from our database please click here {/b} {/a}
{/html}




From daemon Wed Sep 11 09:02:09 2002



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Wed, 11 Sep 2002 09:45:21 -0400
Subject: re:3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
This is a two part (unrelated) question:
1. Is there a good software package that will construct 3D images
from TEM serial sections?
2. Is artificial z stretching of very small moderately bright
flourescent spots common when 3D movies are rotated? We are
getting elongated elipses from deconvolved images (0.2 um
stage steps, proper OTF from PSF for 100X lens), rather than
spheres.

Thanks,
Mike D.



From daemon Wed Sep 11 09:02:15 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Wed, 11 Sep 2002 08:55:20 -0500
Subject: What Hallmark does with SEM-EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello:
A short list of stuff that I have been asked to do.
I get asked this question a lot at meetings and such. When we owned a
plating company I used metallurgical procedures and our metallurgical
microscope to cross-section a lot of samples and to measure their
thickness'. The samples that were to thin to measure on the microscope I
used the SEM to measure the thickness., I also used the EDX system to
determine the composition of the different plating layers and core
materials on all the samples. I still do this on plated items that we now
buy from outside companies. I also have used these procedures on different
broken machine parts, plant support ( such as cooling tower tubes and
sprinkler piping with holes in them ), embossing dyes etc.
We make our own gravvure and flexo printing plates and I have used
the SEM-EDX to determine what caused plating defects in the gravvure
printing plates and to evaluate different materials and procedures used to
make flexo plates.
We have several paper, ink, adhesive and printing chemists and I
have supported their work with the SEM-EDX and light microscopes. Papers -
their coatings, fillings, and type of paper fibers all have had o be known
to get them to print right the SEM-EDX has helped a lot..
Inks - have to be matched with the right papers and printing process and
their shapes, sizes ( usually done by a different procedure ) and
composition, the SEM-EDX has been able to help determine possible problems.
I could go on a long time about papers, inks and printing but I
better stop since I have been told that I can be boring about stuff most
people don't care about.
I also use the SEM-EDX to determine particle sizes and composition of
employees dust exposure in manufacturing, and office areas. Government
regulations require special equipment when particle sizes fall below 10
microns. I also get samples of unknown bags of powder from all over - what
it is type of questions. My favorite samples are white stuff scraped from
around bathroom stools in hotels and plants that we are responsible for,
although oversprayed paint on cars parked in our parking lots are cool too.

Terry Ellis.
Hallmark Cards Inc.



From daemon Wed Sep 11 12:57:42 2002



From: kmccourt-at-nrcan.gc.ca ()
Date: Wed, 11 Sep 2002 12:44:37 -0500
Subject: Ask-A-Microscopist: Parent Needs Help with Olympus Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kmccourt-at-NRCan.gc.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
September 11, 2002 at 12:08:12
---------------------------------------------------------------------------

Email: kmccourt-at-NRCan.gc.ca
Name: Stuart McCourt

Organization: Carleton University

Education: Undergraduate College

Location: Ottawa, Ontario Canada

Question: I am writing this for my son. We have an Olympus BHB
microscope. We have been trying to get the manuals for this
instrument but so far no luck. We have may questions. Que. there is a
lens mounted below the condenser that moves with the condensor and
plugs into the microscope. What is it for and when should it be used?
Que. I have been told that the BHB uses short barrel objective lens
not long barrel since I have lenses from a Wild microscope on it and
they do not work correctly how do I know which lens to obtain? Que
the microscope has a phase contrast unit again what objectives should
I be looking at getting? I am looking a gettting a dark feild
condenser will other dark feild condensers work if they fit into the
condenser holder?

Thank you for your help

Regards Keith McCourt

---------------------------------------------------------------------------


From daemon Wed Sep 11 13:05:53 2002



From: Paul.Nolan-at-alcan.com
Date: Wed, 11 Sep 2002 13:59:16 -0400
Subject: Re: What Hallmark does with SEM-EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Remember we are microscopists too so we enjoy the boring stuff MOST people
don't care about.
I did a lot of work at the university i used to work at looking at paper.
The art conservation students had old paper with neat paints and i got to
find out what stuff the used for dyes and pigments.
It was way cool.

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



"Terry E Ellis"
{tellis2-at-hallmar To: microscopy-at-sparc5.microscopy.com
k.com} cc:
Subject: What Hallmark does with SEM-EDX?
09/11/02 09:55
AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

snip


I could go on a long time about papers, inks and printing but I
better stop since I have been told that I can be boring about stuff most
people don't care about.

Terry Ellis.
Hallmark Cards Inc.








From daemon Wed Sep 11 14:32:17 2002



From: PHILLIPS,NANCY (HP-Corvallis,ex1) :      nancy_phillips-at-hp.com (by way
Date: Wed, 11 Sep 2002 14:20:20 -0500
Subject: HP TEM Engineering Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Please do not contact me about this position, but follow the instructions
below.

Nancy Phillips

Hewlett-Packard Company

The Hewlett-Packard company is a leading global provider of computing and
imaging solutions and services for businesses and homes. The company focuses
on capitalizating on the opportunities of the Internet and the proliferation
of electronic services.
HP has 85,400 employees worldwide in 120 countries. The total revenue from
continuing operations was $42,5 billion in the 1999 fiscal year.
For detailed information about HP, its products and services can be found on
the World Wide Web at http://www.hp.com.

HP is an equal opportunity employer dedicated to affirmative action and
workforce diversity.
For more information about employment opportunities at HP, visit
http://jobs.hp.com

Job Number : 810270
Job Title : TEM Engineer
Department : Research & Development

United States : Oregon - Corvallis

Job Description:

In this job you will be working in an analytical laboratory within an HP
development site. We have a world-class analytical laboratory with a wide
variety of equipment. Our purpose is to support our HP Inkjet and emerging
business partners with world-class analytical information to help solve
complex materials, process, and product issues.

This is a Transmission Electron Microscopy (TEM) engineering position to
work with a new state of the art TEM w/Field Emission, EDS, PEELS, and dual
beam FIB sample preparation facilities. You will be developing and applying
TEM, EELS, and other related applications as assigned. This will involve
client consultations, sample preparation, running analysis on the
appropriate tool/s, interpreting the results, providing a written report to
the client with a review, lead or work in project teams delivering and
documenting new applications or technologies. You will also be expected to
serve as the primary TEM technical contact and develop working partnerships
with other departments within the organization and HP. At times you will be
asked to lead teams for initiating and developing existing and/or new
technology. You will be expected to use statistical concepts to develop and
execute experiments to support R&D and manufacturing, and maintain current
knowledge of technology trends and developments in the Electron Microscopy
community, specifically as this applies to TEM techniques.


Minimum Qualification :

Ph.D in Material Science, Physics, or related disciplines.

Experience includes: two-five years TEM experience including but not limited
to: STEM, EELS (GIF, EFTEM), EDS, and Diffraction studies. With experience
on semiconductors, metals, polymers, ceramics, in the form of bulk,
thinfilms, and single crystals using a variety of sample prep techniques
including FIB. Ultramicrotomy experience desired. General knowledge of
SEM/EDS, Light Microscopy, XRD, Low dose EM and Tomography, and other
analytical techniques. Ability to think outside the analytical box, good
communicator, advanced computer skills, and self-motivated.


How do you apply ?

The quickest and most efficient way for us to process your resume is that
you submit your resume to Hewlett-Packard by using the on-line application
form. You will easily find the application form on http//www.jobs.hp.com.

please include the specific job ID number 810270 on top of your resume, or
in your cover letter when applying for this function.
If your interest in Hewlett-Packard was instigated by either a recruitment
event or an advertisement, you should also include the specific event ID#
(if it was provided to you at the event) or the advertisement ID#..
An advertisement ID# is typically part of the text within the Ad.

For information about additional job opportunities at HP, we recommend you
use our on-line keyword search engine.This tool is available on
http://www.jobs.hp.com
You will be contacted if your resume fits the current position(s).If not,
your application is put into a central database, accessible by any manager
location.
Each time we have a job opening, your information will be checked to see if
it matches the requirements for the open positions.
Your information will remain on our central database for six months. This
means that you will only have to apply to us every six months. (You will be
contacted if your resume fits the current position(s).If not, your
application is put into a central database, accessible by any manager
location).


From daemon Wed Sep 11 15:32:38 2002



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Wed, 11 Sep 2002 15:24:46 -0500
Subject: Hallmark SEM-EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


To all;
I forgot - from Hallmarks perspective we can protect our trade
secrets and patents from disclosure or discovery and can control the timing
of when the projects need to be done based on production needs.
Terry



From daemon Wed Sep 11 16:39:31 2002



From: Zhiping Luo :      luo-at-mic.tamu.edu
Date: Wed, 11 Sep 2002 16:09:57 -0500
Subject: Re: trainee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

Training of new users is an interesting work. During the past year since I
moved to the Texas A&M, I have been training more than a dozen new users of
graduate students, undergraduate students and postdocs on the JEOL 2010 TEM.
My experience is to keep the training as simple as possible.

In the beginning, in order to let the new users have a basic TEM theoretical
background, they are required to read some chapters and respond to a
questionnaire. Then for the hands-on training, I use a simple protocol to
follow up and work with the new users until they feel comfortable working
alone. Usually only about 5 sessions are needed for a new user. While I
still keep myself available to assist them to solve some particular
problems.

Since such a training is simple and the users are only responsible for few
things to do with the operation, I get more and more users with the
microscope.

Best regards,

Zhiping Luo
Research Scientist
Microscopy and Imaging Center, BSBW
Texas A&M University, College Station, TX 77843-2257
Phone: (979) 845-1129 FAX: (979) 847-8933
E-mail: luo-at-mic.tamu.edu http://www.tamu.edu/mic




From daemon Thu Sep 12 07:32:23 2002



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 12 Sep 2002 08:18:25 -0600
Subject: Re: 3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mike,

For the 3-D reconstruction, you could try Volocity™
(Version 1.4.1, Improvision). We used it succesfully to
produce 3-D images of Toxoplasma Gondii parasites
(Ref.: Nature 2002 Aug 1;418(6897):548-52 - check
the movies in the supplemental materials).


The software is for use mainly with confocal light
microscopy, but can be adapted easily for EM. It
is quite expensive (} 20K), so only worth it if you
intend to use it a lot.

Best

Marc

Mike Delannoy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
} This is a two part (unrelated) question:
} 1. Is there a good software package that will construct 3D images
} from TEM serial sections?
} 2. Is artificial z stretching of very small moderately bright
} flourescent spots common when 3D movies are rotated? We are
} getting elongated elipses from deconvolved images (0.2 um
} stage steps, proper OTF from PSF for 100X lens), rather than
} spheres.
}
} Thanks,
} Mike D.

--
Marc Pypaert
Department of Cell Biology,
Center for Cell and Molecular Imaging and
Ludwig Institute for Cancer Research,
Yale University School of Medicine
333 Cedar Street
New Haven CT 06520
Tel : (203) 785 3681
Fax : (203) 785 7446




From daemon Thu Sep 12 07:32:28 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 12 Sep 2002 08:25:44 -0400
Subject: What Hallmark does with SEM-EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Terry;

Thank you for the explanation. Today I can say I learned something.

Peter

-----Original Message-----
} From: Terry E Ellis [mailto:tellis2-at-hallmark.com]
Sent: Wednesday, September 11, 2002 9:55 AM
To: microscopy-at-sparc5.microscopy.com


Hello:
A short list of stuff that I have been asked to do.
I get asked this question a lot at meetings and such. When we owned a
plating company I used metallurgical procedures and our metallurgical
microscope to cross-section a lot of samples and to measure their
thickness'. The samples that were to thin to measure on the microscope I
used the SEM to measure the thickness., I also used the EDX system to
determine the composition of the different plating layers and core
materials on all the samples. I still do this on plated items that we now
buy from outside companies. I also have used these procedures on different
broken machine parts, plant support ( such as cooling tower tubes and
sprinkler piping with holes in them ), embossing dyes etc.
We make our own gravvure and flexo printing plates and I have used
the SEM-EDX to determine what caused plating defects in the gravvure
printing plates and to evaluate different materials and procedures used to
make flexo plates.
We have several paper, ink, adhesive and printing chemists and I
have supported their work with the SEM-EDX and light microscopes. Papers -
their coatings, fillings, and type of paper fibers all have had o be known
to get them to print right the SEM-EDX has helped a lot..
Inks - have to be matched with the right papers and printing process and
their shapes, sizes ( usually done by a different procedure ) and
composition, the SEM-EDX has been able to help determine possible problems.
I could go on a long time about papers, inks and printing but I
better stop since I have been told that I can be boring about stuff most
people don't care about.
I also use the SEM-EDX to determine particle sizes and composition of
employees dust exposure in manufacturing, and office areas. Government
regulations require special equipment when particle sizes fall below 10
microns. I also get samples of unknown bags of powder from all over - what
it is type of questions. My favorite samples are white stuff scraped from
around bathroom stools in hotels and plants that we are responsible for,
although oversprayed paint on cars parked in our parking lots are cool too.

Terry Ellis.
Hallmark Cards Inc.



From daemon Thu Sep 12 07:35:52 2002



From: alicia.ortega-at-colorado.edu (by way of Ask-A-Microscopist)
Date: Thu, 12 Sep 2002 07:26:06 -0500
Subject: Ask-A-Microscopist: etch samples of polycrystalline NiTi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (alicia.ortega-at-colorado.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
September 11, 2002 at 22:14:48
---------------------------------------------------------------------------

Email: alicia.ortega-at-colorado.edu
Name: Alicia Ortega

Organization: CU Boulder

Education: Undergraduate College

Location: Westminster, CO, USA

Question: I have been trying to etch samples of polycrystalline NiTi
to bring out grain boundaries so I can determine the grain size.
Before etching I have been preparing the samples by polishing them
down to a 0.25 micon diamond paste. The etchant I am using is
3HNO3+2H2O+1HF.

I tried leaving the etchant on for anywhere from 1 sec to 2 min, but
nothing seems to work. I was wondering if anyine knows about or has
any experience with ethcing NiTi and might know how long this
particular etchant should take?

Thanks so much for any help you can provide!

regards
Alicia Ortega

---------------------------------------------------------------------------


From daemon Thu Sep 12 07:40:28 2002



From: Kevran44-at-aol.com
Date: Thu, 12 Sep 2002 08:34:20 EDT
Subject: looking for Jeol SEM & TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am looking to buy JEOL used SEM & TEM. If you know of any good used
840,845,848, or newer SEM or 1200 EX , 1010, 2000 JEOL TEM or STEM please
contact Dan at.

Daniel F. Connors
321-726-0669
321-544-5754
danieleds-at-aol.com


From daemon Thu Sep 12 08:00:10 2002



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Thu, 12 Sep 2002 08:51:49 -0400
Subject: SEM Upgrade PGT System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
Does anyone out there uses Avalon 8000 or Spirit system by PGT? I am trying
to get some positives and negatives about the systems.
Or any other suggested systems that have EDS, Digital Imaging, X-ray
mapping, pc based. The upgrade for Amray 1830I with EDS and WDS detectors.

Any info is appreciated.

Pavel Lozovyy
atcsem-at-earthlink.net



From daemon Thu Sep 12 08:02:29 2002



From: zaluzec-at-microscopy.com
Date: Thu, 12 Sep 2002 07:52:48 -0500
Subject: Surplus Equipment : Re: looking for Jeol SEM & TEM:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Daniel

You can also look at the Surplus Equipment pages

http://www.msa.microscopy.com/SurplusEquipment

Nestor
Your Friendly Neighborhood SysOp




From daemon Thu Sep 12 10:40:51 2002



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Thu, 12 Sep 2002 10:32:25 -0500
Subject: re:3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Mike,
Regarding your first question; the IMOD software package comes to mind
(http://bio3d.colorado.edu/imod/). It is a free software package developed
for SGI UNIX which has also been ported to Linux (standard PC hardware) and
probably compiles on other UNIX platforms also. A place to look for other
UNIX/Linux based freeware/shareware solutions for processing
multidimensional data sets is http://www.cs.ubc.ca/spider/ladic/software.html.
MS Windows native solutions tend to be proprietary (VoxBlast is
one package which comes to mind), and I'm not familiar with most of them,
although I believe ImageJ has some image alignment algorithm plugins, and
it has a number of stack manipulation utilities. Once the images are
aligned, the stack can be processed to a number of formats depending on
what is required by your volume rendering software.
Artificial stretching of spherical fluorescent sources in the
z-axis can arise from a number of sources. The first thing that comes to
mind is the point spread function--if the sample in question is mounted in
a media with a refractive index significantly different from that which the
point spread function was determined with then spherical abberation may
distort the resultant image.
Another source of distortion can be the fluorescent sphere
itself-if the sphere is large enough, and is composed of a material of a
refractive index different from the surrounding media, the fluorescent bead
can act like a ball lens and weird images result.
Hardware can always be a culprit. If the stage position feedback
is encoded by the stepper motor only then it is difficult to tell how much
the stage is really moving. In other words, if the stepper recieves a
signal to move 2microns, and it turns 2 microns but the coupling to the
stage z rack slips, then the stage just moved less than it was supposed
to. On a couple of our microscopes the focus drive is actually a bearing
friction coupling and it is capable of slipping. The computer, however,
thinks the stage moved the correct distance, so the spacing between
successive images in a stack is off. So if the computer thinks it moved 8
microns but it only really moved 6 microns then the image will be
stretched. If a linear encoder is providing feedback to the software this
problem should be minimized.
Lastly, the rendering software needs to have the correct
information-if the spacing between individual images in a stack is somehow
entered incorrectly, the voxels may be stretched.
Hope this helps.
Regards,
Karl G.


At 09:45 AM 9/11/2002 -0400, Mike Delannoy wrote:
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_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Thu Sep 12 11:54:53 2002



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Subject: How's It Goin' ..... ?

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From daemon Thu Sep 12 12:28:33 2002



From: Louise_Harner-at-albint.com
Date: Thu, 12 Sep 2002 13:22:01 -0400
Subject: re:3d software and zstretch

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Mike -

I downloaded the demo version of "3-D Doctor" but haven't yet had time to
try it out on my images. You might want to look at their website and see if
it looks promising for your work. I think the software package runs about
$5000.
http://www.ablesw.com/3d-doctor

Other sites that might be of interest (again I haven't had time to check
these out):
http://bio3d.colorado.edu/imod/ IMOD - freeware works in LINUX or on
Silicon Graphics computer
www.isi.uu.nl/people/michael/vr.htm VolumeJ - freeware Java
(multiplatform, Windows compatible)

Neither I nor the company I work for have any financial interest in any of
the above. I've just been looking around for ways to do reconstructions.

- Louise


Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com



|---------+----------------------------}
| | Mike Delannoy |
| | {delannoy-at-jhmi.ed|
| | u} |
| | |
| | 2002/09/11 09:45 |
| | AM |
| | |
|---------+----------------------------}
} --------------------------------------------------------------------------------------------------------------|
| |
| To: microscopy listserver {Microscopy-at-sparc5.microscopy.com} |
| cc: |
| Subject: re:3d software and zstretch |
} --------------------------------------------------------------------------------------------------------------|




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Hello,
This is a two part (unrelated) question:
1. Is there a good software package that will construct 3D images
from TEM serial sections?
2. Is artificial z stretching of very small moderately bright
flourescent spots common when 3D movies are rotated? We are
getting elongated elipses from deconvolved images (0.2 um
stage steps, proper OTF from PSF for 100X lens), rather than
spheres.

Thanks,
Mike D.









From daemon Thu Sep 12 13:11:36 2002



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 12 Sep 2002 11:00:44 -0400
Subject: High-pressure freezer vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,
I will be purchasing a high-pressure freezer. I know that Balzers makes
one, but I do not know whether there are other vendors. If anyone
(including/especially vendors) has experience with either Balzers or other
brands of high-pressure freezer, would you please contact me off-list? TIA.
Yours,
Bill Tivol



From daemon Thu Sep 12 14:17:32 2002



From: Steve Beck :      becks-at-sunynassau.edu
Date: Thu, 12 Sep 2002 15:08:56 -0400
Subject: Philips EM 300 Service

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

Can anyone suggest an independent service engineer who could perform a
routine maintenance/PM on our Philips EM 300 (we are located just east of
New York City on Long Island)? The scope is currently down with vacuum
problem that I believe is related to poor water circulation (I have cleaned
out a couple of filters in the plumbing down behind the panel in the front
leg space of the scope without success). It should be noted that our
greatest problem with service personnel is the Nassau County requirement
that the individual carry $1,000,000 in liability insurance in order to
work on campus.

Please contact me offline if you have any leads!

Regards,

Steve

Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Thu Sep 12 14:57:24 2002



From: Karin Pruessner :      kprue-at-mse.ufl.edu
Date: Thu, 12 Sep 2002 15:50:37 -0400
Subject: TEM: field cancellation

Contents Retrieved from Microscopy Listserver Archives
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Dear list members,

we have some problems with electromagnetic AC and DC fields in the room
where our JEOL 2010F is installed. Therefore, I am currently looking into
field cancellation systems. Installation of a Helmholtz cage has been
recommended by the company who initially did the site survey. I would like
to hear about any user's experience (positive and negative) with it and
possible alternatives. I would be especially interested, if such a system
can deal with interference from multiple and changing fields. Any
advice/comment on this would be greatly appreciated, please contact me
off-line if appropriate. Vendors suggestions are also welcome.

Karin Pruessner, University of Florida



From daemon Thu Sep 12 15:38:52 2002



From: Larry Hanke :      hanke-at-mee-inc.com
Date: Thu, 12 Sep 2002 15:29:46 -0500
Subject: Re: Ask-A-Microscopist: etch samples of polycrystalline NiTi

Contents Retrieved from Microscopy Listserver Archives
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Alicia:

We etch NiTi alloys with a solution of 10 ml HF, 25 ml HNO3,
and 150 ml water. Etching time is 15 to 30 sec. I suspect
that your solution may be more uniformly dissolving the
alloy without delineating the structure. Also, you should
etch this alloy very quickly after polishing. Natural
passivation will inhibit etching if you delay too long
between final polish and etching. A fine oxide polish may
also be needed to get a really good polish that will show a
true structure.

Good luck.
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870




From daemon Thu Sep 12 16:08:51 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 12 Sep 2002 14:03:28 -0700
Subject: Re: SEM Upgrade PGT System

Contents Retrieved from Microscopy Listserver Archives
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You may want to dig into this more deeply. If I recall,
the 1830i has integrated x-ray and does not support
a separate system. There was some special thing done
to PC10 frame buffer that caused this.

Make sure your SEM will accept a separate x-ray. If
J9 is not used or if it has the External Beam Interface
plate installed, you might have a chance.

gary


At 05:51 AM 9/12/2002, you wrote:
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From daemon Thu Sep 12 16:31:13 2002



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 12 Sep 2002 15:18:01 -0600
Subject: RE: 3d software and zstretch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Mike,

if you are intrested in trying our analySIS software with the 3D module,
please contact me offline. The module allows reconstruction from serial
sections. Check our web site for more information.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,
This is a two part (unrelated) question:
1. Is there a good software package that will construct 3D images
from TEM serial sections?
2. Is artificial z stretching of very small moderately bright
flourescent spots common when 3D movies are rotated? We are
getting elongated elipses from deconvolved images (0.2 um
stage steps, proper OTF from PSF for 100X lens), rather than
spheres.

Thanks,
Mike D.









From daemon Fri Sep 13 07:06:56 2002



From: Microshaw-at-aol.com
Date: Fri, 13 Sep 2002 07:58:51 -0400
Subject: Ben Franklin & Flash Photos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


} } In a message dated Thu, 5 Sep 2002 20:48:49 +1000, adavis-at-saipan.com writes:
} }
} } } As we enjoy great advantages from the inventions of others we should
} } } be glad of an opportunity to serve others by any invention
} } } of
} } } ours, and this we should do freely and generously.
} } } -- Benjamin Franklin
} } }
} }
} } Alan-
} } I'll start out by saying that Benjamin Franklin solved the
} } controversy over patenting DNA sequences by major pharmaceutical
} } firms and research companies. These companies point out that they
} } have spent millions in research and want to make proprietary their
} } DNA sequence results. I've always said, as did Franklin here, that
} } the hundred years of scientific research, and all of the government
} } funding and grants that laid the foundations of microbiology were
} } accessed at no cost by the pharmaceutical companies and research
} } companies. Should they not pay the estates of Pasteur, Koch, Semmelweis, and
} } Lister, as well as Rosalind Carter who died of cancer from her work
} } in x-ray crystallography to discover the DNA molecule? Many people
} } contributed to the eventual result published by Watson and Crick-
} } those who weighed the 4 bases that comprise DNA, those who
} } determined it's components. Ben Franklin had it right. I have no
} } problem with pharmaceutical companies or private research companies
} } c!
} harging as much as they want for the products of their research- but
} they ought not restrict the knowledge.
} }
} } Now- about a flash unit in base. Easiest way is to get a small
} } mirror and rig it just above the light in the base. Mount your
} } flash pointed at it, and trip it via camera or manually. If the
} } mirror is small enough, you'll still get enough light from the
} } base-lamp up through the condenser to focus and compose the shot.
} } The flash (set it on auto) will give you what you need on the film.
} } If you want to see how I did mine- look at
} } http://members.aol.com/moresciencestuff/microstuff.html
} } bottom of the page.
} }
} } Good luck-
} } Mike Shaw
} } New Jersey


From daemon Fri Sep 13 07:36:13 2002



From: JHoffpa464-at-aol.com
Date: Fri, 13 Sep 2002 08:25:46 -0400
Subject: Re: Philips EM 300 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


my advice call FEI service. it will cost but will be worth it in the long run.
john


From daemon Fri Sep 13 10:50:02 2002



From: Margaret M. Miller :      millermm-at-uthscsa.edu
Date: Fri, 13 Sep 2002 10:40:10 -0500
Subject: EM plate film processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I am interested in any information concerning darkroomless processing of
4489 EM film. I know of 2 processors on the market-one stand alone and the
other water connected.
Your knowledge and input on automated film processing of 4489 film is much
appreciated.

Peggy Miller
UTHSCSA



From daemon Fri Sep 13 11:38:57 2002



From: rcmoretz-at-att.net
Date: Fri, 13 Sep 2002 16:31:04 +0000
Subject: Re: Ben Franklin & Flash Photos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Actually, the name was not Carter, it was Franklin.

Roger Moretz
--
Where the world is only slightly
less weird than it actually is.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } } In a message dated Thu, 5 Sep 2002 20:48:49 +1000, adavis-at-saipan.com writes:
} } }
} } } } As we enjoy great advantages from the inventions of others we should
} } } } be glad of an opportunity to serve others by any invention
} } } } of
} } } } ours, and this we should do freely and generously.
} } } } -- Benjamin Franklin
} } } }
} } }
} } } Alan-
} } } I'll start out by saying that Benjamin Franklin solved the
} } } controversy over patenting DNA sequences by major pharmaceutical
} } } firms and research companies. These companies point out that they
} } } have spent millions in research and want to make proprietary their
} } } DNA sequence results. I've always said, as did Franklin here, that
} } } the hundred years of scientific research, and all of the government
} } } funding and grants that laid the foundations of microbiology were
} } } accessed at no cost by the pharmaceutical companies and research
} } } companies. Should they not pay the estates of Pasteur, Koch, Semmelweis, and
} } } Lister, as well as Rosalind Carter who died of cancer from her work
} } } in x-ray crystallography to discover the DNA molecule? Many people
} } } contributed to the eventual result published by Watson and Crick-
} } } those who weighed the 4 bases that comprise DNA, those who
} } } determined it's components. Ben Franklin had it right. I have no
} } } problem with pharmaceutical companies or private research companies
} } } c!
} } harging as much as they want for the products of their research- but
} } they ought not restrict the knowledge.
} } }
} } } Now- about a flash unit in base. Easiest way is to get a small
} } } mirror and rig it just above the light in the base. Mount your
} } } flash pointed at it, and trip it via camera or manually. If the
} } } mirror is small enough, you'll still get enough light from the
} } } base-lamp up through the condenser to focus and compose the shot.
} } } The flash (set it on auto) will give you what you need on the film.
} } } If you want to see how I did mine- look at
} } } http://members.aol.com/moresciencestuff/microstuff.html
} } } bottom of the page.
} } }
} } } Good luck-
} } } Mike Shaw
} } } New Jersey
}


From daemon Fri Sep 13 13:07:00 2002



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Fri, 13 Sep 2002 10:53:08 -0700
Subject: Leica service on West Coast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Does anyone offer service for a Leitz Aristoplan on the West Coast? We
have an Aristoplan with the Variophot photohead that generates its own
dirt and haze in the sealed optics, and needs the focus re-lubed. I'm
tired of shipping it to Leica in New Jersey just to have the internal
surfaces lined with thumbprints and new dirt to replace the old.

Regards,
glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************


From daemon Fri Sep 13 14:18:07 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 13 Sep 2002 14:10:12 -0500
Subject: LM - uneven field in brightfield

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have an uneven field of illumination in my microscope when I do
bright field microscopy. I have tried to align the bulb to even out
the field but I am not having much luck. I am using 20x and 63x Plan
Apo objectives and the scope is in "perfect" Kohler illumination. I
know how to subtract this variation with image processing but it
would obviously be better to minimize this variation in the original.
What I am wondering is how much variability should I expect in a well
aligned, clean microscope? If one takes a digital image of a blank
field using either a 20x 0.5 NA objective or a 63x PlanApo oil NA
1.25 objective, how wide of a pixel intensity would you find
acceptable? Is there something I should be concerned with other than
the bulb alignment? TIA, Tom


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Sep 13 14:33:19 2002



From: Margaret M. Miller :      millermm-at-uthscsa.edu
Date: Fri, 13 Sep 2002 14:26:31 -0500
Subject: 4489 film processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I am interested in any information concerning darkroomless processing of
4489 EM film. I know of 2 contained, auto processors on the market-one
stand alone and the other water connected.
Your knowledge and input on automated film processing of 4489 film will be
appreciated.

Peggy Miller
UTHSCSA



From daemon Fri Sep 13 15:47:06 2002



From: Margaret Brannigan :      brannign-at-asrr.arsusda.gov
Date: Fri, 13 Sep 2002 16:41:02 -0400
Subject: TEM: Need gold conjugate source

Contents Retrieved from Microscopy Listserver Archives
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--
Hi,

I'm trying to find a commercial source of goat anti-chicken (serum
proteins, not egg) antibody conjugated to 10 nm gold particles. I
would deeply appreciate any recommendations anyone can give me. If
you have personal knowledge of the product's quality, that would be
even better, but not necessary--at this point, I'm just trying to
find out who offers it.

Thank you,

Margaret Dienelt

Plant Pathologist/Electron Microscopist


Floral and Nursery Plants Research Unit
National Arboretum
Agricultural Research Service, USDA

Beltsville Agriculural Research Center
Bldg. 010A Rm. 248
10300 Baltimore Avenue
Beltsville Maryland 20705

(301) 504-6097



From daemon Fri Sep 13 17:36:46 2002



From: sstouden-at-thelinks.com
Date: Fri, 13 Sep 2002 17:28:12 -0500 (CDT)
Subject: Re: Ben Franklin & Flash Photos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I would like to forword this entire email to members of a legal list
server regards copyrights and patents.

Who that has the authority could give me permission to do?

also from where did the Franklin quote originate?
sterling

On Fri, 13 Sep 2002 rcmoretz-at-att.net-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Actually, the name was not Carter, it was Franklin.
}
} Roger Moretz
} --
} Where the world is only slightly
} less weird than it actually is.
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } } } In a message dated Thu, 5 Sep 2002 20:48:49 +1000, adavis-at-saipan.com writes:
} } } }
} } } } } As we enjoy great advantages from the inventions of others we should
} } } } } be glad of an opportunity to serve others by any invention
} } } } } of
} } } } } ours, and this we should do freely and generously.
} } } } } -- Benjamin Franklin
} } } } }
} } } }
} } } } Alan-
} } } } I'll start out by saying that Benjamin Franklin solved the
} } } } controversy over patenting DNA sequences by major pharmaceutical
} } } } firms and research companies. These companies point out that they
} } } } have spent millions in research and want to make proprietary their
} } } } DNA sequence results. I've always said, as did Franklin here, that
} } } } the hundred years of scientific research, and all of the government
} } } } funding and grants that laid the foundations of microbiology were
} } } } accessed at no cost by the pharmaceutical companies and research
} } } } companies. Should they not pay the estates of Pasteur, Koch, Semmelweis, and
} } } } Lister, as well as Rosalind Carter who died of cancer from her work
} } } } in x-ray crystallography to discover the DNA molecule? Many people
} } } } contributed to the eventual result published by Watson and Crick-
} } } } those who weighed the 4 bases that comprise DNA, those who
} } } } determined it's components. Ben Franklin had it right. I have no
} } } } problem with pharmaceutical companies or private research companies
} } } } c!
} } } harging as much as they want for the products of their research- but
} } } they ought not restrict the knowledge.
} } } }
} } } } Now- about a flash unit in base. Easiest way is to get a small
} } } } mirror and rig it just above the light in the base. Mount your
} } } } flash pointed at it, and trip it via camera or manually. If the
} } } } mirror is small enough, you'll still get enough light from the
} } } } base-lamp up through the condenser to focus and compose the shot.
} } } } The flash (set it on auto) will give you what you need on the film.
} } } } If you want to see how I did mine- look at
} } } } http://members.aol.com/moresciencestuff/microstuff.html
} } } } bottom of the page.
} } } }
} } } } Good luck-
} } } } Mike Shaw
} } } } New Jersey
} }
}



From daemon Fri Sep 13 22:49:28 2002



From: Chaoying Ni :      cni-at-udel.edu
Date: Fri, 13 Sep 2002 23:39:57 -0400 (EDT)
Subject: FESEM - comments sought

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello lister,

I was told that at this past M&M meeting, both Hitachi and JEOL
demonstrated their newest FESEM models - Hitachi S-4800 and JSM-7400F. I
was wondering if any of you could make comments concerning these two or
other FESEM versions. I'm especially interested in the quality of
corresponding cold FEGs, data base management, and proprietary
technologies such as ExB, energy filter, and in-lens or semi in-lens
detectors. Both on and off line responses are welcome. Thanks in advance!

****************************************
Chaoying Ni
The W.M. Keck Electron Microscopy Facility
College of Engineering
University of Delaware
Newark, DE 19716

(302) 831-8354 (O); -2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************



From daemon Sat Sep 14 09:07:02 2002



From: Chaoying Ni :      cni-at-udel.edu
Date: Sat, 14 Sep 2002 09:52:54 -0400 (EDT)
Subject: TEM: rough edge of FEG beam crossover at high mag

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

On our recently installed FETEM, I have noticed that when the
magnification is x800k-1.5M, the beam crossover loses its smoothness of
the edge. The shape also becomes off-circular. The alignment doesn't seem
to be an issue (checked both by myself and service engineer). My question
is whether this is normal or acceptable (scope is still under warranty).
Can anyone shed some light on this? Thanks!

****************************************
Chaoying Ni
The W.M. Keck Electron Microscopy Facility
College of Engineering
University of Delaware
Newark, DE 19716

(302) 831-8354 (O); -2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************



From daemon Sat Sep 14 12:59:23 2002



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Sat, 14 Sep 2002 13:50:09 -0400
Subject: Re: FESEM - comments sought

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I would like to see a summary of the responses, if you
would not mind.

Thank you,
Darrell




From daemon Sun Sep 15 23:58:46 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 15 Sep 2002 22:13:21 -0700
Subject: Re: Thanks to all who attended ICEM15

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Luc
It was pleasure to be at ICEM-15. I was impressed how well everything were
organized and program was great. Thanks! Sergey

At 03:43 PM 9/10/02 +0200, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Sun Sep 15 23:58:52 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 15 Sep 2002 22:00:26 -0700
Subject: Fwd: High-pressure freezer vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello Bill

I am not an expert in this area, but just completed Cryo-EM course and had
chance to work on Balzers and Leica's instruments. Leica's is compact, use
a fraction of LN2 Balzers used and more easy to operate. Could not say
anything about sample's quality - I could not get them from Elanie for a
few month. I would suggest you ask for demo and compare results
side-by-side. Best wishes, Sergey

} Date: Thu, 12 Sep 2002 11:00:44 -0400
} From: Bill & Sue Tivol {wtivol-at-earthlink.net}
} Subject: High-pressure freezer vendors
} To: microscopy list {microscopy-at-sparc5.microscopy.com}
} User-Agent: Microsoft Outlook Express Macintosh Edition - 5.01 (1630)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Sep 16 05:24:38 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 16 Sep 2002 05:23:11 -0700 (PDT)
Subject: Re: Leica service on West Coast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chaoying,

Such distortion of the beam is usually caused by charging of contaminants on
the surface of the condenser aperture. Changing or cleaning the aperture may
help. When the beam diameter is very small (like the one needed for the
magnification range you mentioned) such roughness of the beam edge is
considered normal. Try switching the condenser apertures and see if there is
big difference in the beam shape between different apertures. If one of the
apertures is "dirty" there will be big difference in distortion between it
and the other apertures. If all the apertures show comparable roughness -
don't bother to change or clean them ... the situation may worsen.
The problem of condenser aperture charging is much more severe for FEG than
LaB6 guns. The FEG is much brighter source producing very small crossover
(high coherency). If you were using LaB6 in the magnification range you
mention you'll usually work with the beam in crossover.

Hope this helps.

Best regards,

Rado

---------------------------------------------------------------------
Radostin Danev, Ph.D.
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
phone: 0564-55-7813
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------

----- Original Message -----
} From: "Chaoying Ni" {cni-at-udel.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, September 14, 2002 10:52 PM


Have you considered Otto Breiner Instruments? He's
well known here in the Detroit area. Here's the
business card information:

246 Heather Heights
Monrovia, CA 91016
ph 818-357-3859
fax 818-358-1209

Stu Smalinskas
SKF North American Technical Center
Plymouth, Michigan

__________________________________________________
Do You Yahoo!?
Yahoo! Finance - Get real-time stock quotes
http://finance.yahoo.com


From daemon Mon Sep 16 07:31:44 2002



From: Emmanuelle Roux :      eroux-at-caprion.com
Date: Mon, 16 Sep 2002 08:24:35 -0400
Subject: gold conjugate anti-chicken

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Margaret,
You will find donkey anti-chicken IgY (H+L) conjugated to gold at Bio/Can Scientific (Jackson Immunoresearch); they have 6, 12 and 18 nm gold conjugates. You can reach them at one of these numbers: 800-367-5296; 610-869-4024; 800-387-8125;
or websites: www.jacksonimmuno.com or www.biocan.com
I haven't use those so I can't tell you much more than that.
Good luck!
Emmanuelle

Emmanuelle Roux, PhD
Senior Scientist
Caprion Pharmaceuticals
7150 Alexander Fleming
St-Laurent, H4S 2C8
Quebec, Canada
Tel: 514-940-3600 ext. 3773
Fax: 514-940-3620



From daemon Mon Sep 16 12:45:44 2002



From: Anna Logvinova :      alogvinova-at-buckinstitute.org
Date: Mon, 16 Sep 2002 10:35:16 -0700
Subject: Job posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Fellow or Research Associate - Morphology Core

The Buck Institute for Age Research aims to increase the healthy years of life through clinically relevant biomedical research on the biology of aging and age-associated diseases. Established as an independent, nonprofit research facility, the Buck Institute is located in Marin County north of the San Francisco and brings a new focus to the science of aging with a world-class Faculty.

Candidates with a strong commitment to understanding aging and the causes of age-related disease are sought for the technical position of a Postdoctoral Fellow or Research Associate to join its Morphology Core facility. Duties will include sample preparation using basic histology and immunochemistry techniques, image analysis, and minor equipment maintenance. Successful candidate will instruct and assist the users of the facility and will actively participate in the ongoing research projects. Applicants should have experience in the basic histology/immunocytochemistry, fluorescence and transmitted light microscopy, and some image analysis. Experience in electron microscopy will be a plus. Excellent communication and organizational skills and the ability to manage a large workload are a must. Salary will depend upon qualification and experience. Some training will be provided. We offer a competitive salary, excellent benefits, dynamic work environment, and new state-of-the-art facility. To apply, send cover letter and resume with MC/RA on the subject line of the e-mail, to hr-at-buckinstitute.org
You are welcome to contact me if you have any questions about this position.

Anna Logvinova, M.D.
Morphology Core Manager
Buck Institute
8001 Redwood Blvd
Novato, CA 94948
www.buckinstitute.org

alogvinova-at-buckinstitute.org


From daemon Mon Sep 16 12:54:17 2002



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Mon, 16 Sep 2002 12:48:10 -0500
Subject: MM02: proceedings and reprints.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I still did not received proceedings and reprints from MM'02 meeting.
What is the status of their mailing?

Regards,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy




From daemon Mon Sep 16 13:08:36 2002



From: tbargar-at-unmc.edu
Date: Mon, 16 Sep 2002 13:01:37 -0500
Subject: Telemicroscopy for the classroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




__________________

I run the core EM facility at UNMC in Omaha, NE. and I would like to set up
some sort of teleconferencing or telemicroscopy link with the local biology
classes. The teachers and myself of course have no experience in this. I
would like to hear from anyone who has been involved in this sort of
arrangement. All help and advice will be greatly appreciated.

Tom Bargar
402-559-7347
tbargar-at-unmc.edu




From daemon Mon Sep 16 15:52:22 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 16 Sep 2002 14:05:15 -0700
Subject: Re: how to produce extremely hi res templates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chris
To test/calibrate my laser diffractometer I used to use copper/nickel
150-300 mesh sheets. We used that material, also, to punch 3 mm EM grids in
the early era of EM. Originally it comes from electronic industry where
used in 'magnetron' production. You probably could obtain similar material
from EM grids suppliers (may be expensive if special order). You could
print such 'mesh' by contact printing on hi-res film. Such sample
delivered very nice diffraction pattern. It's not such funny as Esher
figures but nice test-sample for laser diffractometer. Another idea: to use
old friend - protection technique. Make Hi-res print-out of your funny
images, use conventional film enlarger in 'opposite' way: load unexposed
film in the film-holder and project your print-out on the film. It'll take
some experimenting with focusing, illumination, exposure etc. You may also
use convention SLR 35mm camera with hi-res/hi contrast sci grade film to
take pictures. Your drawings should be large enough. I think, it's
simplest way to do so. If need details, you may contact me off-line. Good
luck, Sergey.

At 08:58 AM 9/3/02 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Mon Sep 16 18:14:00 2002



From: Walsh, Sharon :      Sharon.Walsh-at-bannerhealth.com (by way of
Date: Mon, 16 Sep 2002 18:01:59 -0500
Subject: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in purchasing a scanner as a back-up in the event our
CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
have checked on the Agfa Duoscan and found that it is no longer in
production. Is anyone using another reasonably priced scanner and if
so, which one? Any information would be greatly appreciated.

Thank-you.

Sharon Walsh
System Technical Specialist
Laboratory Sciences of Arizona
Good Samaritan Medical Center
Phoenix, Az.
(602)239-2343


From daemon Mon Sep 16 21:06:50 2002



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 16 Sep 2002 19:00:51 -0700
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


At 04:01 PM 9/16/2002, you wrote:

} I am interested in purchasing a scanner as a back-up in the event our CCD
} camera should fail or to scan old 3 1/4 x 4 negatives, if necessary. We
} have a Philips EM208S TEM and an AMT 2K CCD camera. I have checked on the
} Agfa Duoscan and found that it is no longer in production. Is anyone
} using another reasonably priced scanner and if so, which one? Any
} information would be greatly appreciated.
}
} Thank-you.
}
} Sharon Walsh
} System Technical Specialist
} Laboratory Sciences of Arizona
} Good Samaritan Medical Center
} Phoenix, Az.
} (602)239-2343


Check out the Nikon CoolScan 8000. It will just barely
handle TEM negs. But will definitely handle 35mm and MF formats.

gary g.



From daemon Tue Sep 17 07:28:58 2002



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 17 Sep 2002 08:21:53 -0400
Subject: RE: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The Microtek Artixscan 2500f is the mechanical twin of the
Agfa Duoscan T2500. It has been updated with both IEEE1394
(Firewire) and SCSI interfaces and an improved dynamic range.
The carrier design, like the Duoscan is glassless. The 4x5
carrier works well to hold 3.25x4 TEM negs. We have a PDF I am
happy to send or visit http://www.microtekusa.com/as2500f.html

The Artixscan 1100 has a similar design but with lower
optical resolution and SCSI interface only.
http://www.microtekusa.com/as2500f.html

We have customers very happy with both.

George

George Laing
National Graphic Supply
ph 800.223.7130 x3109
f 800.832.2205
email scisales-at-ngscorp.com



I am interested in purchasing a scanner as a back-up in the event our
CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
have checked on the Agfa Duoscan and found that it is no longer in
production. Is anyone using another reasonably priced scanner and if
so, which one? Any information would be greatly appreciated.

Thank-you.

Sharon Walsh
System Technical Specialist
Laboratory Sciences of Arizona
Good Samaritan Medical Center
Phoenix, Az.
(602)239-2343






From daemon Tue Sep 17 07:28:59 2002



From: Susan Carbyn :      CarbynS-at-agr.gc.ca
Date: Tue, 17 Sep 2002 08:12:54 -0400
Subject: Disposable Lab Coats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I thought I'd send a message for a quick response (hopefully I don't get daggers thrown at me) - with respect to disposable lab coats. I was asked about the possibility of using them, for people coming into the lab to work on equipment, or visitors wanting to observe techniques. As part of the New Safety regulations, I have to make sure people have access to proper protective clothing. It has been argued that lab coats are contaminated when a person goes into our lab, because of the arsenic, and other contaminants we use. I know this question sounds a bit ridiculous to some, but it was suggested to me, that we have disposable lab coats for students, visitors, and any one else entering the lab.

Does anyone have any helpful thoughts on this, and could provide some helpful advice?

Thanks in advance,

Susan

Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, N.S. B4N 1J5
Canada

Phone (902) 679-5535
Fax (902) 679-2311

E-Mail: carbyns-at-em.agr.ca



From daemon Tue Sep 17 07:48:22 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 17 Sep 2002 08:46:54 -0400
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




"Walsh, Sharon (by way of MicroscopyListserver)" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am interested in purchasing a scanner as a back-up in the event our
} CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
} necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
} have checked on the Agfa Duoscan and found that it is no longer in
} production. Is anyone using another reasonably priced scanner and if
} so, which one? Any information would be greatly appreciated.
}
} Thank-you.
}
} Sharon Walsh
} System Technical Specialist
} Laboratory Sciences of Arizona
} Good Samaritan Medical Center
} Phoenix, Az.
} (602)239-2343

Get an Epson 2450 with the transparency adapter. Does a great job for about
$400.
The Nikon 8000 (and Minolta Dimage Scan Multi Pro) will only do a 6cm x 9cm
portion of a
TEM negative.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Sep 17 08:13:20 2002



From: Davis Baird :      db-at-sc.edu
Date: Tue, 17 Sep 2002 08:45:38 -0400
Subject: STM/AFM: History

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

I am a philosopher/historian of science/technology, with a particular
interest in scientific instruments. I am now starting research into
the history of the development of scanning tunneling microscopy and
atomic force microscopy. I am interested in the range of issues that
involve these developments, but I am particularly interested in (and
having some difficulty finding information about) how various
commercial instrument makers picked up and developed these
technologies. Here I would like to know who did this, how they
interacted with the academic and commercial research communities, and
how innovations were exchanged between these interacting communities.

Thanks for whatever help members can provide.

Davis Baird
--
********************************************************************
Davis Baird
db-at-sc.edu
http://www.cla.sc.edu/PHIL/faculty/baird/INDEX.HTM
Editor, Techne: Journal of the Society for Philosophy and Technology
Professor and Chair
Department of Philosophy
University of South Carolina
Columbia, SC 29208 USA
(803) 777-4166
FAX (803) 777-9178
********************************************************************


From daemon Tue Sep 17 09:03:44 2002



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Tue, 17 Sep 2002 15:09:53 +0100
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Sharon

Microtek supply a whole range of glassless scanners of the Agfa duoscan
type (i'm not sure if they just took over some of the Agfa Duoscan
range).

The scanners of most interest to you are probably:
Duoscan style with conventional flatbed and seperate glassless holders
for up to about 5x10 or 8x10 inches (not cms)
Microtek Scanmaker 8700 cheapest - comes as a basic model or PRO version
with better software
Microtek Artixscan 1100
Microtek Artixscan 2500f most expensive
There are more but they seem to go up to A3 or do other things I don't
want.

dedicated large format film scanner :
(will take negatives up to 4x5 inches)
Microtek Artixscan 4500t

All have some glassless holders and range from 1200 dpi up to 2500 dpi
with Dmax from 3.4 up to 3.9; colour depth is 42 bit which should
translate to 14 bit B&W. Interfaces vary from SCSI, USB and firewire.
Prices appear to be from 600 UK pounds to 3,100 UK pounds.

You could start with the website at http://www.microtekusa.com (check
out products and the 3 ranges of scanners) and perhaps run a search
engine for more background information.

I don't have a Microtek at present and I have no connection with the
company but I am probably going to get one of the cheaper ones. If
anyone has any user experience of these scanners I would be interested
in their opinions.


Good luck.

Malcolm Haswell
E.M. Unit
School of Health, Natural and Social Sciences
University of Sunderland
UK







"Walsh, Sharon (by way of MicroscopyListserver)" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am interested in purchasing a scanner as a back-up in the event our
} CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
} necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
} have checked on the Agfa Duoscan and found that it is no longer in
} production. Is anyone using another reasonably priced scanner and if
} so, which one? Any information would be greatly appreciated.
}
} Thank-you.
}
} Sharon Walsh
} System Technical Specialist
} Laboratory Sciences of Arizona
} Good Samaritan Medical Center
} Phoenix, Az.
} (602)239-2343


From daemon Tue Sep 17 09:26:13 2002



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Tue, 17 Sep 2002 09:19:40 -0500
Subject: Ahesive for serial sections- simple solution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listies;

I recently posted a querry requesting a good source of adhesive for serial
sections of plastic blocks.

I found that scotch tape dissolved in xylene works fine.

Thanks contributors,
Tim Quinn
University of Kansas
Program Assistant/Microscopists/Lab Mgr
Ornithology Dept.
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556/785-331-4107
tquinn-at-ku.edu


From daemon Tue Sep 17 09:40:19 2002



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 17 Sep 2002 10:33:27 -0400
Subject: RE: Need gold conjugate source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Margaret,

A invaluable source for finding antibodies is Linscott's Directory. Their
address is 815 Whitney Way, Petaluma, CA 94954 (707-763-7098).
http://www.linscottsdirectory.com

They list not only the antibodies, but where to order them. There is a fee
for the catalog and/or the online directory. I highly recommend it.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

} -----Original Message-----
} From: Margaret Brannigan [SMTP:brannign-at-asrr.arsusda.gov]
} Sent: Friday, September 13, 2002 4:41 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM: Need gold conjugate source
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} --
} Hi,
}
} I'm trying to find a commercial source of goat anti-chicken (serum
} proteins, not egg) antibody conjugated to 10 nm gold particles. I
} would deeply appreciate any recommendations anyone can give me. If
} you have personal knowledge of the product's quality, that would be
} even better, but not necessary--at this point, I'm just trying to
} find out who offers it.
}
} Thank you,
}
} Margaret Dienelt
}
} Plant Pathologist/Electron Microscopist
}
}
} Floral and Nursery Plants Research Unit
} National Arboretum
} Agricultural Research Service, USDA
}
} Beltsville Agriculural Research Center
} Bldg. 010A Rm. 248
} 10300 Baltimore Avenue
} Beltsville Maryland 20705
}
} (301) 504-6097
}


From daemon Tue Sep 17 09:48:46 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 17 Sep 2002 10:34:21 -0400
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} I am interested in purchasing a scanner as a back-up in the event
} our CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
} necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera.
} I have checked on the Agfa Duoscan and found that it is no longer in
} production. Is anyone using another reasonably priced scanner and
} if so, which one? Any information would be greatly appreciated.
} ***************

hi Sharon,
I have an EPSONExpression 1600 Pro with a transilluminator. I have
had good results with it. It has true optical resolution of 1600
dpi, was very easy to install (has Fire Wire, USB and SCSI2
interfaces). It was also VERY reasonably priced, which was good for
me because I had $4500 with which to upgrade my whole
computer/printer/scanner system. I does not have a film holder for
EM plates, but it does have a focus adjustment to account for the
film lying on the scanner bed and i have not had any trouble with
moire lines, etc.
I"m sure the more expensive scanners have better options/feature, but
this was very good for its price.
Lee

}


--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Sep 17 10:31:16 2002



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 17 Sep 2002 10:20:25 -0500
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I like the Epson 1680 - it has a Dmax of 3.6 - you need the highest
Dmax you can get when you are working with EM negatives. Remember
this is a log scale so each 0.3 difference is 10x better. We scan in
the 16 bit mode, then adjust levels using Photoshop and then convert
to 8 bit so that we get max contrast range without all those annoying
gaps between pixel densities.


--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Sep 17 13:11:41 2002



From: James Talbot :      james-at-ktgeo.com
Date: Tue, 17 Sep 2002 13:04:06 -0500
Subject: ESEM or SEM in DFW Area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear List-

I have a potential client who is looking to rent time on an ESEM
(preferably) or SEM in the Dallas Fort Worth Texas (preferred) or
Houston Texas area. He has a corroded copper plug ( { 1 inch in
diameter) that he would like to get some EDS data on. If you are
interested please reply off-line.

Thank you,
James Talbot

K/T GeoServices, Inc.
Bulk and Clay Mineralogy by X-ray Diffraction
(940) 597-9076
web site: http://www.ktgeo.com/




From daemon Tue Sep 17 15:31:51 2002



From: JHoffpa464-at-aol.com
Date: Tue, 17 Sep 2002 16:21:27 -0400
Subject: Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


hi. was interested to know if anyone has used the lynx tissue processor to stain grids. if so, any comments or suggestions.
john


From daemon Tue Sep 17 15:31:57 2002



From: JHoffpa464-at-aol.com
Date: Tue, 17 Sep 2002 16:22:30 -0400
Subject: lynx tissue processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


hi, was interested to know if anyone has used the lynx tissue processor to stain grids. if so, any comments or suggestions.
john



From daemon Tue Sep 17 15:32:36 2002



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 17 Sep 2002 15:25:36 -0500
Subject: Software: speech recognition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I apologize is this is not strictly a microscopy question. A
microscopist colleague/friend of mine is interested in purchasing a
high quality speech recognition software. The individual is
handicapped and does not full use of their hands and would be using
the software for scientific writing (primarily describing microscopy
findings). Although I favor the Macintosh system (since they would
also use the computer for image processing and publishing), I am
looking for the best software to assist this individual. Any
recommendations would be appreciated. Many thanks.

JB

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Sep 17 17:17:47 2002



From: jerzy.gazda-at-amd.com
Date: Tue, 17 Sep 2002 17:08:42 -0500
Subject: TEM automated sample prep tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample preparation in our TEM laboratory and would like to hear your opinions on the following semi-automated approaches. The lab processes nearly a 1000 TEM samples per year, mostly cross-sections of CMOS devices on Si wafers. We use manual polishing, dimpling, ion mills, and FIBs tools, however we don't have enough people to handle the load and will not see an increase in headcount in the near future. I would appreciate any and all input on the following combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From root Tue Sep 17 17:47:07 2002
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Dear Listers,

We are forced to evaluate methods of improving efficiency of sample preparation in our TEM laboratory and would like to hear your opinions on the following semi-automated approaches. The lab processes nearly a 1000 TEM samples per year, mostly cross-sections of CMOS devices on Si wafers. We use manual polishing, dimpling, ion mills, and FIBs tools, however we don't have enough people to handle the load and will not see an increase in headcount in the near future. I would appreciate any and all input on the following combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From daemon Tue Sep 17 19:13:24 2002



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 17 Sep 2002 19:14:31 -0400
Subject: RE: Telemicroscopy for the classroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tom,

Short of getting instruments that are pre-pared for remote, you can
do the following.

Start at Oak Ridge to see the high end of your dreams.

http://www.ms.ornl.gov/htmlhome/mauc/default.htm

Then take a trip to BUGSCOPE:

http://bugscope.beckman.uiuc.edu/

And finally, stop off at the HOME of BugScope:

http://www.itg.uiuc.edu/technology/, where you can find
other outreach programs to emulate.

Finally, finally, a real favorite, and a very worthwhile stop:

http://www.sci.sdsu.edu/EM_Facility/index.html

Hope this helps to answer some of your questions.

Regards,

Fred Monson


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson-at-wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/

THINKING IS MUCH MORE DIFFICULT THAN MEMORIZING.


} ----------
} From: "tbargar-at-unmc.edu"-at-sparc5.microscopy.com
} Sent: Monday, September 16, 2002 2:01 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Telemicroscopy for the classroom
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} __________________
}
} I run the core EM facility at UNMC in Omaha, NE. and I would like to set
} up
} some sort of teleconferencing or telemicroscopy link with the local
} biology
} classes. The teachers and myself of course have no experience in this. I
} would like to hear from anyone who has been involved in this sort of
} arrangement. All help and advice will be greatly appreciated.
}
} Tom Bargar
} 402-559-7347
} tbargar-at-unmc.edu
}
}
}
}


From daemon Tue Sep 17 19:29:03 2002



From: Dwight Arrieche (IIBCA) :      darriech-at-cumana.sucre.udo.edu.ve (by way
Date: Tue, 17 Sep 2002 19:17:54 -0500
Subject: LM lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Dear List Servers

I need some help for cleaning or removing oil from LM lenses.
What is the recommended method and solvents for this task?

Thanks

D. Arrieche
IIBCA-UDO
darriech-at-cumana.sucre.udo.edu.ve


From daemon Tue Sep 17 20:12:42 2002



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 18 Sep 2002 13:04:42 +1200
Subject: Re: scanner (and for geological thinsections)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




This reply may not be relevant to your query, but I have recently found
out how good current domestic flatbed scanners are.

I bought a CanoScan D1250UF, the F means it has a little fluorescent
light built in to the lid, for scanning 35mm film/slides. Less than
US$200.

The holder can easily accept two pieces of polaroid film as well as a
standard petrological thinsection, and, with the thing set for 2400 dpi, in
a few minutes (less, if your computer has USB-2), you have a digital
image of the whole thinsection in transmitted light with crossed polars,
at good enough quality to blowup to A4 size!

Sure makes it easy to find your way around when the thinsection is in
the EPMA, bound to be other uses as well eg depicting textures.

cheers

rtch

}
} I am interested in purchasing a scanner as a back-up in the event our
} CCD camera should fail or to scan old 3 1/4 x 4 negatives, if
} necessary. We have a Philips EM208S TEM and an AMT 2K CCD camera. I
} have checked on the Agfa Duoscan and found that it is no longer in
} production. Is anyone using another reasonably priced scanner and if
} so, which one? Any information would be greatly appreciated.
}

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Sep 18 07:26:24 2002



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Wed, 18 Sep 2002 13:15:33 +0100
Subject: TEM automated sample prep tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jerzy,
we have a similar workload to you, but in III-V's (we have looked at
475 TEM samples since April 1). We have only 2 people (myself and one
other) doing all the sample prep, TEM and report writing. Much of our work
is straightforward thickness measurements and cross-sections, and we have no
FIB.

Do you have a Gatan PIPS or similar low-angle ion mill, with the ability
to mill from predominantly one direction? I've found a great way of making
cross sections using this machine combined with precision(ish) cleaving,
cutting out several stages from the 'standard' way of doing things. Typical
InP cross sections take less than an hour to do; with the right kind of
sample (i.e. not site specific in 2 dimensions) it is possible to stack them
up and do up to 10 at a time. It's straightforward to hit features down to
20um in size, but if you want to keep the specimen you really have to do one
at a time. (It's impossible to get several specific sites thin at the same
time, meaning you have to look at the first region that becomes electron
transparent, then destroy it while getting the next one thin, etc..) Si
takes longer since it ion mills so much more slowly, but the few I've done
still take less than 90 mins from getting the sample to looking at it in the
TEM.

I've been thinking of writing this up and publishing it somewhere, since
it has saved us a huge amount of time and effort. I'm sure that if you were
working with Si all the time you'd be able to optimise the procedure still
further. I'll try to get some words together describing the procedure, with
pictures, if you like.

FIB is great for very specific sites (less than 10um or so, depending on the
structure) and I do use one in a tame academic's lab every so often, but for
fast cross sections with lots of thin area I can't beat 'standard' methods.
However I appreciate that Si geometries are often several orders of
magnitude smaller and more complex than III-Vs, and site specific samples
are likely to be a much bigger proportion of your workload.

Good luck,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com




-----Original Message-----
} From: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Sent: 17 September 2002 23:09
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample
preparation in our TEM laboratory and would like to hear your opinions on
the following semi-automated approaches. The lab processes nearly a 1000 TEM
samples per year, mostly cross-sections of CMOS devices on Si wafers. We use
manual polishing, dimpling, ion mills, and FIBs tools, however we don't have
enough people to handle the load and will not see an increase in headcount
in the near future. I would appreciate any and all input on the following
combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with
those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White
Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





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From daemon Wed Sep 18 07:57:58 2002



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 18 Sep 2002 08:55:11 -0400
Subject: fish embryos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear List:

Does anyone have experience fixing and embedding fish embryos in
plastics (epoxies or methacrylates) for serial sectioning? Advice,
references and warnings are all appreciated!

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Sep 18 10:34:50 2002



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Wed, 18 Sep 2002 08:21:31 -0700 (PDT)
Subject: Re: Software: speech recognition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi John,

I purchased "Via Voice" because I wanted to use it on a Mac and of course
the choices for Mac were more limited. I also didn't want to spend a lot
of money. For me, this seems to be a farely sophisticated program for a
very reasonable price. It is, however, difficult to use if there is other
noise in the room. I can only use it in a private office.

Bob
Research Scientist
U of Washington

On Tue, 17 Sep 2002, John J. Bozzola wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I apologize is this is not strictly a microscopy question. A
} microscopist colleague/friend of mine is interested in purchasing a
} high quality speech recognition software. The individual is
} handicapped and does not full use of their hands and would be using
} the software for scientific writing (primarily describing microscopy
} findings). Although I favor the Macintosh system (since they would
} also use the computer for image processing and publishing), I am
} looking for the best software to assist this individual. Any
} recommendations would be appreciated. Many thanks.
}
} JB
}
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}
}



From daemon Wed Sep 18 10:34:51 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 18 Sep 2002 11:17:56 +0200
Subject: Philips EM 201 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

Can anyone suggest an independent service engineer who could perform a
routine maintenance on our Philips EM 201 (we are located in New Haven,
Connecticut)? The scope is currently down with a vacuum problem.

Please contact me offline if you have any leads!

Regards,

Stefan


°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6210

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Wed Sep 18 10:34:52 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Wed, 18 Sep 2002 17:24:30 +0200
Subject: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Does anyone have any good electropolishing recipes for Co-Ni alloys? We
have a Struers Tenupol. Suggestions for voltage and temperature would
be good too.

Thanks in advance


--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Wed Sep 18 10:44:29 2002



From: Oakley, Jeff :      oakleyj-at-rayovac.com
Date: Wed, 18 Sep 2002 10:37:30 -0500
Subject: book search / plating anomalies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a reference that documents various anomalies associated with electroplating and electroless plating of metals such as gold, copper, nickel, zinc, etc., as seen through the SEM.

I took a tour through Amazon's bookshelves but didn't see anything that matched what I was looking for.

TIA,

Jeff Oakley


From daemon Wed Sep 18 11:07:22 2002



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Wed, 18 Sep 2002 09:54:14 -0500
Subject: Re: LM lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Dwight,
I posted a very similar on the MSA Listserver about this time last
year. The listserv archives can be searched using the interface at
http://www.msa.microscopy.com/MicroscopyListserver/SearchMLArchive.html,
however I can't seem to get the results I'm looking for using it--I posted
a summary of the responses I received last year on 9/26.
I managed to pull up the whole exchange in my Eudora archives, so
I've appended the summary in the hope it provides you with the info you are
after. I have been using spectroscopic grade MeOH dried with 4 angstrom
molecular sieves in conjunction with cotton tipped swabs and filter
paper. Usually a confident swab in one direction across the lens while
simultaneously turning the shaft of the swab removes contamination. I use
a new swab for every wipe, and the swab is dampened with only enough MeOH
so that I can watch the trailing edge of the solvent rapidly evaporate
behind the swab as I move it across the lens. Contamination should be
trapped in the cotton tip of the swab--a similar process can be performed
using strips of lens tissue dragged across the lens if you are afraid the
swab might scratch the lens surface. I observe the whole operation with
the objective placed under a dissecting microscope so that I can see what
I'm doing, and whether it has been effective.
The lens cleaning summary follows:

Greetings,
I would like to thank all who offered their advise regarding the
cleaning of objectives, and remedies for misused optics. I had included
the suggestion of sonicating in acetone for comic relief (the rest of my
friend's suggestion was that at least then I could put a hook through the
hole where the lens used to be and use it as an ornament), however I do
appreciate the concern which was expressed by many of you. Your
suggestions have been very helpful and are much appreciated. I even learned
how to make purple Sparkle glass cleaner clear (apparently the purple dye
is photolabile).
It appears that lens cleaning is a craft, the art of which is
developed through trial and experience. There are many different
approaches to the cleaning of objective lenses, however the underlying
philosophies may be grouped under three major headings: 1.) physical
interaction with the lens surface, 2.) the use of aqueous or water soluble
cleaning solutions, and 3.) the dark art of organic solvents.
An interesting non-solvent method I have come across is to
fracture a piece of expanded polystyrene (packaging material) and to use
the freshly exposed surface to clean the lens (press into the lens and
rotate). This method was recommeded by Leitz (now Leica) in the
past. Apparently the method is effective and will not scratch the lens.
For light duty cleaning, various formulations of aqueous
solvent/detergent mixtures seem to be popular. These cleaning solutions
include Kodak lens cleaner, Windex, and Sparkle. Some people recommend
cutting the concentration of these solutions with distilled water
(50%). Sparkle seems to have somewhat of a cult following-it is alcohol
and ammonia free-and it is what we have been using here for some time. In
all cases, care must be taken to drag the contamination and grit away from
the lens using lens tissue. Work from the inside of the lens out, and make
sure not to smear contamination across areas which you have just cleaned
(use the lens tissue to trap the contamination, not just move it
around). The aqueous detergent methods seem to be safe for lens
coatings. Oil objectives do not need to be cleaned with detergent each
use-wiping the oil off with lens tissue should suffice as long as different
formulations of immersion oil are not being used on the same
objectives. Apparently, immersion oils from different sources can react to
produce a sort of sludge which is then more difficult to remove. Also,
old-fashioned natural immersion oils such as pine oil have a volatile
component which will evaporate and leave a gummy residue behind. Inverted
microscopes may benefit from the use of little "diapers" for the objective
lenses which absorb excess oil and prevent it from leaking back into the
objective. Preventative maintenence is the goal here.
The use of organic solvents is not for the faint of heart, however
some brave souls manage to use these methods without incident. The less
fortunate tell tales of leaking lenses (a problem particularly on inverted
scopes) and even lens components dropping out of the barrel of the
objective. Attention to proper technique is important. High quality
cotton tipped applicators are favored for cleaning objectives with strong
solvents-some people make their own and some people purchase them. A
common theme is to begin at the center of the lens and work outward, slowly
rotating the cotton swab so as to pick up the contamination rather than
drag it about the lens. Using a dry applicator or reusing any portion of
the applicator can scratch the lens. The cement which holds the lens
together is easily dissolved using some of these solvents, and so great
care must be taken not to "drown" the lens in solvent. The applicator
should be neither dry nor saturated with solvent. It is important not to
touch the cotton and contaminate it with oil from one's fingers. In an
alternative method, one gently and unidirectionally drags a single-use
strip of lens paper dampened in one spot with the solvent across the
lens. Favored solvents include: lighter fluid (apparently used by some
service reps), anhydrous ether, a mixture of diethylether and benzene,
spectroscopy grade chloroform, isopropanol, methanol and xylene. There are
others, to be sure. A common final touch is to fog the lens using
distilled water from one's breath, and polish with one unidirectional swipe
of a high quality lens paper.
I hope this summary helps those of you who have expressed interest
in the subject of objective cleaning and once again I thank everyone for
thier input.
Best Regards,
Karl G.

At 07:17 PM 9/17/2002 -0500, Dwight Arrieche (IIBCA)" (by way wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_______________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illiniois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Wed Sep 18 11:19:01 2002



From: Lesley Weston :      lesley-at-vancouverbc.net
Date: Wed, 18 Sep 2002 09:12:10 -0700
Subject: Re: LM lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


You could try a 50:50 mix of Windex (or any clear window-cleaning fluid) and
distilled water. Use Kimwipes or cotton buds or cloth, never Kleenex, with
many gentle wipes rather than a few vigorous ones, and be careful to dry the
lens at the end. Hope this helps.

Lesley Weston.



on 17/09/2002 5:17 PM, Dwight Arrieche (IIBCA) (by way of
MicroscopyListserver) at darriech-at-cumana.sucre.udo.edu.ve wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear List Servers
}
} I need some help for cleaning or removing oil from LM lenses.
} What is the recommended method and solvents for this task?
}
} Thanks
}
} D. Arrieche
} IIBCA-UDO
} darriech-at-cumana.sucre.udo.edu.ve
}



From daemon Wed Sep 18 12:21:23 2002



From: Tom Moninger :      thomas-moninger-at-uiowa.edu
Date: Wed, 18 Sep 2002 12:12:42 -0500
Subject: Whole lung

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone experience with embedding whole human lung in paraffin or
methacrylate, especially if the lung was previously air-dried while inflated?
Thanks, Tom

Thomas Moninger (thomas-moninger-at-uiowa.edu)
University of Iowa Central Microscopy Research Facility (www.uiowa.edu/~cemrf)
View expressed are my own.



From daemon Wed Sep 18 12:36:32 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 18 Sep 2002 13:27:15 -0400
Subject: TEM automated sample prep tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Richard,
I would like to remind you that there will be a TEM symposium at next year's M&M meeting. If you want to write it up, that may be the perfect place.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Richard Beanland [mailto:richard.beanland-at-bookham.com]
Sent: Wednesday, September 18, 2002 8:16 AM
To: 'jerzy.gazda-at-amd.com'
Cc: Microscopy-at-sparc5.microscopy.com


Hi Jerzy,
we have a similar workload to you, but in III-V's (we have looked at
475 TEM samples since April 1). We have only 2 people (myself and one
other) doing all the sample prep, TEM and report writing. Much of our work
is straightforward thickness measurements and cross-sections, and we have no
FIB.

Do you have a Gatan PIPS or similar low-angle ion mill, with the ability
to mill from predominantly one direction? I've found a great way of making
cross sections using this machine combined with precision(ish) cleaving,
cutting out several stages from the 'standard' way of doing things. Typical
InP cross sections take less than an hour to do; with the right kind of
sample (i.e. not site specific in 2 dimensions) it is possible to stack them
up and do up to 10 at a time. It's straightforward to hit features down to
20um in size, but if you want to keep the specimen you really have to do one
at a time. (It's impossible to get several specific sites thin at the same
time, meaning you have to look at the first region that becomes electron
transparent, then destroy it while getting the next one thin, etc..) Si
takes longer since it ion mills so much more slowly, but the few I've done
still take less than 90 mins from getting the sample to looking at it in the
TEM.

I've been thinking of writing this up and publishing it somewhere, since
it has saved us a huge amount of time and effort. I'm sure that if you were
working with Si all the time you'd be able to optimise the procedure still
further. I'll try to get some words together describing the procedure, with
pictures, if you like.

FIB is great for very specific sites (less than 10um or so, depending on the
structure) and I do use one in a tame academic's lab every so often, but for
fast cross sections with lots of thin area I can't beat 'standard' methods.
However I appreciate that Si geometries are often several orders of
magnitude smaller and more complex than III-Vs, and site specific samples
are likely to be a much bigger proportion of your workload.

Good luck,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356398
http://www.bookham.com




-----Original Message-----
} From: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Sent: 17 September 2002 23:09
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample
preparation in our TEM laboratory and would like to hear your opinions on
the following semi-automated approaches. The lab processes nearly a 1000 TEM
samples per year, mostly cross-sections of CMOS devices on Si wafers. We use
manual polishing, dimpling, ion mills, and FIBs tools, however we don't have
enough people to handle the load and will not see an increase in headcount
in the near future. I would appreciate any and all input on the following
combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with
those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White
Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





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From daemon Wed Sep 18 12:39:10 2002



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 18 Sep 2002 13:32:39 -0400
Subject: TEM automated sample prep tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am curious. If you have an FIB, why don't you set up several sample areas to be milled overnight, come in a clean them up, and then use the pluck-out system? If you have multiple samples, you could cut them down or cleave them and mount them on a holder and then use the auto milling program.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: "jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com
[mailto:"jerzy.gazda-at-amd.com"-at-sparc5.microscopy.com]
Sent: Tuesday, September 17, 2002 6:09 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,

We are forced to evaluate methods of improving efficiency of sample preparation in our TEM laboratory and would like to hear your opinions on the following semi-automated approaches. The lab processes nearly a 1000 TEM samples per year, mostly cross-sections of CMOS devices on Si wafers. We use manual polishing, dimpling, ion mills, and FIBs tools, however we don't have enough people to handle the load and will not see an increase in headcount in the near future. I would appreciate any and all input on the following combinations of methods and equipment.

* wafering saw and FIB

vs.

* semi-automated polisher and ion mill

vs.

* precision cleaving and FIB

Thank you in advance for your opinions and accounts of your experiences with those approaches.

Regards,

Jerzy



******************************************************
Jerzy Gazda, Ph.D. Advanced Micro Devices
Supervising Engineer 5204 E. Ben White Blvd. - MS 613
PCAL - AIM Section Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453 FAX: (512) 602-7470
jerzy.gazda-at-amd.com
******************************************************





From daemon Wed Sep 18 13:03:38 2002



From: Jiang Liu :      jiangliu24680-at-hotmail.com
Date: Wed, 18 Sep 2002 13:56:07 -0400
Subject: AFM phase imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:

I have been working on phase imaging of high impact polystyrene (HIPS), a
composite of polystyrene and polybutadiene rubber particles, with DI AFM
TappingMode. I have tried different microtoming conditions to optimize the
cutting and therefore the imaging from those specimens, but with limited
success. I have also found cutting temperature (tried from 0 C to -80 C) is
very important but same temperature results in different cutting qualities,
primarily chatters and local compressions on polystyrene matrix, from
different specimens. However, -40 C is good for any specimens of impact
copolymer of propylene and ethylene.

Does anybody have any experiences, suggestions or comments? Many thanks in
advance.

Jiang Liu, PhD
Research and Technology Center
ATOFINA Petrochemicals
(281) 884-0529
Jiang.liu-at-atofina.com


_________________________________________________________________
Chat with friends online, try MSN Messenger: http://messenger.msn.com



From daemon Wed Sep 18 13:38:26 2002



From: Frida.Maiers-at-co.hennepin.mn.us
Date: Wed, 18 Sep 2002 13:29:59 -0500
Subject: gloves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Working in a clinical lab of a hospital we are under pressure to eliminate
latex gloves in favor of nitrile and now vinyl. Does anyone have
appropriate references to support any of these, none of these or some of
these? Thanks in advance for your help.
F. Maiers




From daemon Wed Sep 18 15:05:17 2002



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Wed, 18 Sep 2002 15:56:40 -0400
Subject: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Sharon,
We have been using an EPSON Expression 1600.
The film holders did not come in 3.5x4 but I use the holder for four
- 4x5s and with 3 sides supported, all is well.
The machine works well, the limitation is my lack of knowledge in
working in Adobe to make the images look their best.
Pat Connelly
Univ. of Pennsylvania, Biology Dept.


From daemon Wed Sep 18 15:12:34 2002



From: JHoffpa464-at-aol.com
Date: Wed, 18 Sep 2002 16:05:47 -0400
Subject: Re: Philips EM 201 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


why an independant service. fei does a great job.
john


From daemon Wed Sep 18 15:39:36 2002



From: robert.fowler-at-tdktca.com
Date: Wed, 18 Sep 2002 16:36:37 -0400
Subject: Re: book search / plating anomalies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



You may want to try "The Electrodeposition Of Tin And Its Alloys" by Dr
Manfred Jordan ISBN 3-87480-118-7 This is an excellent reference with lots
of SEM pictures.

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



"Oakley, Jeff"
{oakleyj-at-rayov To: "MSA listserver"
ac.com} {Microscopy-at-sparc5.microscopy.com}
cc:
09/18/2002 Subject: book search / plating anomalies
11:37 AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am looking for a reference that documents various anomalies associated
with electroplating and electroless plating of metals such as gold, copper,
nickel, zinc, etc., as seen through the SEM.

I took a tour through Amazon's bookshelves but didn't see anything that
matched what I was looking for.

TIA,

Jeff Oakley






From daemon Wed Sep 18 15:57:53 2002



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 18 Sep 2002 16:45:53 +0200
Subject: Re: Philips EM 201 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,

by asking for an independent service I did not want to imply that I ever
was dissatisfied with the Fei/Philips service. In my lab in Germany we
have a CM 10 which is serviced by Fei/Philips for over 10 years and they
always did a great job and sometimes even more than that!
Looking for an independent service for the 201 has more to do with the
age of the instrument. I don´t know if Fei services instruments that
old, but was in the process of contacting them and get this information.

Stefan





From daemon Wed Sep 18 16:21:10 2002



From: evgenia.pekarskaya-at-exxonmobil.com
Date: Wed, 18 Sep 2002 17:13:46 -0400
Subject: Postdoc position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Post doctoral position available at the University of New Orleans/ Advanced
Materials Research Institute (AMRI)

Requirements : Background in Materials Sciences and experience in the
operation of a TEM

Project :
Li-ion batteries are used as power source in portable electronics and
medical devices. After high cycle number the capacity of these batteries
decreases which is partly due to structural instabilities of the cathode
(LiCoO2). The study the crystal structure and lattice defects in LiCoO2
before and after electrochemical cycling is aimed at a better understanding
of the failure mechanisms leading to the reduced performance.

Contact :
Heike Gabrisch
Assistant Professor of Chemistry
Department of Chemistry/AMRI
University of New Orleans
New Orleans, LA 70148

hgabrisc-at-uno.edu... phone (504 )280-1122 ... fax (504) 280-3185 ...


**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355




From daemon Wed Sep 18 16:49:03 2002



From: David Henriks :      henriks-at-southbaytech.com
Date: Wed, 18 Sep 2002 14:32:49 -0700
Subject: Re: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Ian:

I have a recipe I can share from Bernie Kestel which was used on our
Model
550 Jet Polisher. I'm sure it won't translate exactly to your
equipment,
but it may be a good starting point. I hope it helps.


Material: Ni-10Co

Electrolyte: 10% HClO4
90% ethanol

Temperature: 0 degrees C

Jet Height: 3.9mm

Pump Speed: 2-4

Volts: 20

Current: 35 mA

Comments: After termination, rinse specimen immediately.

This is from an Argonne National Lab Publication (ANL-80-120) titled
"Polishing Methods for Metallic and Ceramic Transmission Electron
Micrscopy
Specimens" by Bernie Kestel.

I don't know if it is available from Argonne anymore, but I can supply
you
with a copy of the booklet (about 60 pages) if you think it would be
useful. It discusses many different jet polishing techniques although
the
majority of it is done with one of our Model 550 Jet Polishers.

Best regards-

David

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


Ian MacLaren wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear all,
} Does anyone have any good electropolishing recipes for Co-Ni alloys? We
} have a Struers Tenupol. Suggestions for voltage and temperature would
} be good too.
}
} Thanks in advance
}
} --
} Ian MacLaren
} Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
} ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/


From daemon Wed Sep 18 16:52:57 2002



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Wed, 18 Sep 2002 16:33:57 -0500
Subject: Re: LM lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello Dwight,
I compiled answers to your question from a previous inquiry a year
or so ago.
Let me know if it helps. I have pasted it in at the end of this
message.
Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242-1324

At 07:17 PM 9/17/2002 -0500, you wrote:
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


BEST SOLVENT FOR REMOVING IMMERSION OIL FROM OBJECTIVE LENS

I asked if xylene was the best solvent for cleaning immersion oil off a
non-immersion objective. Here is the array of responses I received. The
diversity may interest you.

1. We regularly use xylene with a cotton tipped applicator to clean our
lenses and remove immersion oil. This was recommended to us by the
supplier of our lenses and microscopes for a number of reasons. The
primary reason is that xylene effectively 'cuts' the oil, without damaging
the lens. Acetone will affect the lens coating, and isopropyl alcohol only
rinses the oil without effectively removing it.

2. Use alcohol on lens paper. Never use xylene or toluene; these solvents
can dissolve the cement used to seat the various lens elements.

3. I would recommend Sparkle Glass Cleaner. I only use solvents as a very
last resort. The cements are soluble in most solvents. With intractable
grime, I use a cotton tipped applicator moistened with either xylenes or
toluene and shake all excess off. The tip must be moist, not wet or dry,
and make single passes until the grime come off. Use only enough solvent
on the applicator to dampen the surface, not enough to wet.

4. For day-to-day cleaning of lenses, including oil on the 40X, invert an
ocular and examine the surface for cleanliness. If it is clean, don't
clean it. Dust off any loose debris. Check the lens again. Moisten a
cotton tipped applicator in Sparkle and wipe the lens once with a rolling
action to present a new surface and lift off any grit. Discard. Take a
dry applicator and remove the film. Check the lens with an inverted ocular
to see if it is clean. Do no more than is necessary.

5. The care instructions for my immersion oil suggest cleaning with a soft
cloth or lens tissue (no Kimwipes) moistened with ether/alcohol (7:3) or
xylenes. My microscope manuals recommend removing fingerprints using alcohol

6. We use 70 % isopropanol to clean immersion oil off of our lenses.

7. Cleaning with xylene is a little drastic. If the lens eventually gets
xylene in behind the cement it could do some damage and you would have to
send it in for repair. I use Kodak lens cleaner and some lens cleaning
tissues (not Kimwipes) to get the oil off, constantly checking under a
dissecting scope to make sure that it is all removed. This works well on
some expensive lens that we have here.

8. We use Green Soap from the pharmacy. It works best with ultra pure water.

9. Alcohol is usually safer than xylene, but safer still is diluted
detergent, such as "Joy" or similar, followed by water. Use alcohol only
if that doesn't work. Fumes from xylene, toluene, etc. are best avoided.

10. Back in the old days, toluene was a recommended solvent for cleaning
lenses of immersion oil. These days we wipe off excess oil with lens
tissue and then clean the lens with Kodak lens cleaner solution.

11. The best way to clean immersion oil from a lens is to use lighter
fluid. First remove as much oil from the lens with lens paper. Be gentle;
don't rub. Then dip a cotton swab in the lighter fluid and lightly wipe it
across the lens. All the oil will be removed and it will evaporate very
quickly without leaving a residue or streaks. Xylene is not recommended as
it can dissolve the adhesives holding the lens in place.



From daemon Wed Sep 18 17:48:18 2002



From: Greg Mulhollan :      mulhollan-at-extremedevices.com
Date: Wed, 18 Sep 2002 17:40:21 -0500
Subject: Commercial Cathodoluminescence Lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Microscopy Folks,
I am trying to find a commercial or perhaps university lab which will
allow us to so some SEM cathodoluminescence measurements on carbon
field emitters. Any pointers? Thanks,
Greg M.


Gregory Mulhollan, Ph.D.
Senior Scientist
Extreme Devices Inc.
3500 ComSouth Drive
Austin, TX 78744
(512)439-3512 voice
(512)439-3487 fax
mulhollan-at-extremedevices.com



From daemon Wed Sep 18 18:25:49 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 18 Sep 2002 18:18:52 -0500
Subject: back issues of JCB for the asking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Microscopists,
I have a run of Journal of Cell Biology from 1987 through
2001 that I would like to give away. Our library doesn't want them.
Anyone out there interested? Or know of a connection to libraries
somewhere that might be interested?

Thanks,
Tobias Baskin
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Wed Sep 18 23:18:03 2002



From: Donald Werder :      aettinger1984-at-comcast.net
Date: Thu, 19 Sep 2002 00:02:40 -0400
Subject: Re: book search / plating anomalies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Jeff,
I only have experience in imaging electroless copper by TEM. TEM shows
small bubbles in the metal. I don't remember the details as this was
some time ago. I believe they are produced by Hydrogen, which tends to
make the metals more brittle.

Don Werder

Oakley, Jeff wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Sep 19 06:55:37 2002



From: Oparowski, Joseph :      Joseph_Oparowski-at-bose.com
Date: Thu, 19 Sep 2002 07:47:54 -0400
Subject: book search / plating anomalies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Jeff,

You may want to check publications from ASM international
(www.asminternational.org). Start with Volume 5 (10th edition) of the
Metals Handbooks-Surface Engineering. Volume 11 deals with Failure Analysis
and Prevention and may have some examples. Good Luck.


Joseph

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313


-----Original Message-----
} From: Oakley, Jeff [mailto:oakleyj-at-rayovac.com]
Sent: Wednesday, September 18, 2002 11:38 AM
To: MSA listserver


I am looking for a reference that documents various anomalies associated
with electroplating and electroless plating of metals such as gold, copper,
nickel, zinc, etc., as seen through the SEM.

I took a tour through Amazon's bookshelves but didn't see anything that
matched what I was looking for.

TIA,

Jeff Oakley


From daemon Thu Sep 19 07:53:12 2002



From: robert.fowler-at-tdktca.com
Date: Thu, 19 Sep 2002 13:05:59 -0400
Subject: hf antidote gel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

You might want to contact Dr. Fred Hossler at hossler-at-ETSU.Edu. He is very
helpful in these matters.

JD Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Tom Moninger" {thomas-moninger-at-uiowa.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, September 18, 2002 1:12 PM



Hi Folks I am curious if any of you using HF are utilizing the hf antidote
gel. If you are where can I find some in US. Thank You

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com


THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY
TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL
INFORMATION.

If you are not the intended recipient, be advised that any use,
dissemination, distribution or duplication of this transmission is strictly
prohibited. If you received this transmission in error, please notify the
sender immediately by electronic reply to this transmission or by phone
(847-803-6100). Thank you.




From daemon Thu Sep 19 12:11:23 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 19 Sep 2002 12:01:47 -0500
Subject: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
We, being non-material people, need some help with a material sample.
The sample is a high impact polystyrene (HIPS) that contains butadiene
rubber moieties. We need to be able to identify the location of these
rubber moieties and understand that they will stain upon exposure to osmium.
Does anyone have any experience with this type of sample. Would it be
advisable to stain by floating thin sections (on grids) on liquid osmium or
by using osmium vapors? What type of grids would be most desirable to
minimize corrosion by the osmium?

Also, we have a diamond knife made by Diatech in Tennessee. I need to
contact them to obtain information about this knife. Does anyone have a
phone number or other contact information?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Thu Sep 19 12:12:51 2002



From: Mick Thomas :      mgt3-at-ccmr.cornell.edu
Date: Thu, 19 Sep 2002 13:08:29 -0400
Subject: SF6 safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists,

I would be very grateful if someone could offer me advice on how to ensure
that a microscopy room is set up to safely handle a leak of SF6.

Thank you very much for your time and help.

Sincerely,

Mick

-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu



From daemon Thu Sep 19 13:38:07 2002



From: Kai Lorcharoensery :      kai-at-lehigh.edu
Date: Thu, 19 Sep 2002 14:35:47 -0400
Subject: Re: hf antidote gel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The gel is calcium gluconate. You can buy a few tubes from Fisher (HF
antidote gel) or Pharmascience.

Kai Lorcharoensery
Materials Science & Engineering
Lehigh University
5 E. Packer Ave.
Bethlehem, PA 18015

"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com wrote:

} Hi Folks I am curious if any of you using HF are utilizing the hf antidote gel. If you are where can I find some in US. Thank You
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}



From daemon Thu Sep 19 14:17:05 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Thu, 19 Sep 2002 15:15:47 -0400
Subject: Commercial Cathodoluminescence Lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Greg;

One of our folks in my dept. worked at Ohio State in the group that used
cathodeluminescence.

The contact he gave me at Ohio State is below.

Regards,

Peter Tomic
Anadigics, Inc.

Professor Len Brillson
Department of Electrical Engineering



-----Original Message-----
} From: Greg Mulhollan [mailto:mulhollan-at-extremedevices.com]
Sent: Wednesday, September 18, 2002 6:40 PM
To: Microscopy-at-sparc5.microscopy.com


Hi Microscopy Folks,
I am trying to find a commercial or perhaps university lab which will
allow us to so some SEM cathodoluminescence measurements on carbon
field emitters. Any pointers? Thanks,
Greg M.


Gregory Mulhollan, Ph.D.
Senior Scientist
Extreme Devices Inc.
3500 ComSouth Drive
Austin, TX 78744
(512)439-3512 voice
(512)439-3487 fax
mulhollan-at-extremedevices.com



From daemon Thu Sep 19 14:17:42 2002



From: Nancy Cherim :      nac-at-cisunix.unh.edu
Date: Thu, 19 Sep 2002 15:15:18 -0400
Subject: Hitachi H600 available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html





Hello listers,
The University of New Hamphsire has a Hitachi H600 100 kV STEM with an
H-6010 scanning system (working), an Oxford thin window EDS (working) and a
PGT IMIX-PC system that we are in the process of replacing. The microscope
was installed in 1986 and is not operational due to a high voltage buffer
problem (we think). Also, the PGT Sun system needs a new hard drive (we
think). The microscope has 2 sets of 30 plate film cassettes and boxes with
vacuum film desiccator. We will be dismantling the scope soon. Interested
parties looking for microscope parts, or interested in getting this one to
work, please contact me with inquiries/offers off-line. It will be the
donee's responsibility to come and take the microscope or pay removal,
shipping and handling charges. I am open to discuss any offers.
I will also post details on Nestor's surplus equipment list at the MSA
website.

Thanks!


Dr. Jeff Simpson
Electron Microscope Facility
University Instrumentation Center
University of New Hampshire
Kendall Hall
Durham, NH 03824
603-862-2457
jeff.simpson-at-unh.edu

Visit us at http://www.unh.edu/instrumentation-center/about.html



From daemon Thu Sep 19 15:03:38 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 19 Sep 2002 14:55:37 -0500
Subject: SEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I am looking for a very fine grained film to use for capture of SEM
images. We want to use this film in place of the Polaroid Type 55P/N film.
Kodak recommends the Tmax 100 4x5 sheet film.
I would appreciate other recommendations or confirmation that this would
be the film of choice.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Thu Sep 19 15:24:23 2002



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 19 Sep 2002 15:16:00 -0500
Subject: Re: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Debbie,
There is some literature suggesting that ruthenium tetroxide
is better than osmium for this kind of thing. There is a paper by
Trent, Scheinbeim, and Couchaman 1983 Macromols 16: 589-598 that is
very informative. My understanding is that it is usual to vapor
stain this kind of sample.
Hope this helps,
Tobias
}
}
}
} Listers,
} We, being non-material people, need some help with a material sample.
} The sample is a high impact polystyrene (HIPS) that contains butadiene
} rubber moieties. We need to be able to identify the location of these
} rubber moieties and understand that they will stain upon exposure to osmium.
} Does anyone have any experience with this type of sample. Would it be
} advisable to stain by floating thin sections (on grids) on liquid osmium or
} by using osmium vapors? What type of grids would be most desirable to
} minimize corrosion by the osmium?
}
} Also, we have a diamond knife made by Diatech in Tennessee. I need to
} contact them to obtain information about this knife. Does anyone have a
} phone number or other contact information?
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Thu Sep 19 16:21:26 2002



From: Gillies,Concettina :      Gillies-at-up.uchc.edu
Date: Thu, 19 Sep 2002 17:13:27 -0400
Subject: EM Technologist position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Opening for an experienced EM tech. at the Univ. of Connecticut Health
Center.
Prefer candidate with clinical experience.
Details available online at http://employ.uchc.edu/


From daemon Thu Sep 19 16:55:12 2002



From: Tom Murray :      murraytm-at-u.washington.edu
Date: Thu, 19 Sep 2002 14:48:38 -0700
Subject: Philips 300 HT Tank

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Sorry if this is a duplicate, but the first time didn't seem to go through.

I have a HT tank for a Philips 300 which I have no use for. The part number
is 4022 185 00651. It is already crated. I do not know if it is in working
order. The crate has a RMA number on it, but I don't know it was fixed and
returned or if it was never sent in for repairs.

Available to anyone who will cover shipping.

If I don't hear from any interested parties by the end of next week, I will
pursue scrapping it.

Tom
--
Thomas M. Murray Phone: (206)543-2836
Electron Microscopy Center Manager Fax: (206)543-3100
Materials Science & Engineering email: murraytm-at-u.washington.edu
Roberts Hall 130
University of Washington
Seattle, WA 98195



From daemon Thu Sep 19 18:07:22 2002



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 19 Sep 2002 15:55:55 -0700
Subject: Re: SEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Debby,

I would certainly consider Kodak Tech Pan 4415. Speed is dependent on
development but will be slower than T-Max, around 25-32 and grain structure
is very fine.

Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
http://katie.ucdavis.edu
raharris-at-ucdavis.edu




} Listers,
} I am looking for a very fine grained film to use for capture of SEM
} images. We want to use this film in place of the Polaroid Type 55P/N film.
} Kodak recommends the Tmax 100 4x5 sheet film.
} I would appreciate other recommendations or confirmation that this would
} be the film of choice.
}
} Thanks,
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907



From daemon Thu Sep 19 19:21:18 2002



From: HWHAMILT-at-UTMB.EDU (by way of Ask-A-Microscopist)
Date: Thu, 19 Sep 2002 19:07:22 -0500
Subject: Ask-A-Microscopist: Hobby LM Purchase

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (HWHAMILT-at-UTMB.EDU) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
September 19, 2002 at 15:24:08
---------------------------------------------------------------------------

Email: HWHAMILT-at-UTMB.EDU
Name: Dub Hamilton

Organization: Utmb Galveston

Education: Graduate College

Location: galveston Texas

Question: I am interested in purchasing a microscope for 'serious
hobby use'. A number are available at about $500 with a 1.25
adjustable abbe condensers.(looking at Accu-scopy a3025 and LW
Scientific Observer IV) Questions:
1. Is this about right for me?
2. Is Kohler illumination possible with this condenser or is
something eles required?
3.Is achromatic objectives ok for my purpose?

thanks

---------------------------------------------------------------------------


From daemon Thu Sep 19 21:27:23 2002



From: Ian Kaplin :      ian-at-mail.emu.usyd.edu.au
Date: Fri, 20 Sep 2002 12:16:24 +1000
Subject: Os staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


The method we use is to stain in Os vapour for 30 min to an hour on
ultrathin sections. We normally use copper grids without any problems.

Put a couple of drops of 2% stock Os solution in a jar, position the
grids above the liquid (we use an old flexible plastic grid holder)
and leave capped in fumehood for the time.

Leave the jar open to air out after removal of Os at least overnight
before discarding the jar and leave the grids open to air for awhile
before putting in microscope to get rid of Os smell.

--

Ian Kaplin

Electron Microscope Unit
Madsen Bldg F09
The University of Sydney
NSW 2006
Australia

Tel : 61 2 9351 3302
Fax : 61 2 9351 7682


From daemon Thu Sep 19 23:52:43 2002



From: sstouden-at-thelinks.com
Date: Thu, 19 Sep 2002 23:40:17 -0500 (CDT)
Subject: Re: Software: speech recognition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


there is a product called Reachout which was designed forthis.
I used it for a person totally disabled back in 1998.. he had an eyeball
controlled keyboard and could use windows nt very well with it from his
always "on the bak possition. he was paralyzed from his neck down.21
years old at time.
you might search fo it I believe the people that made it were in Florida.
if you need more help let me know.

sterling

On Tue, 17 Sep 2002, John J. Bozzola wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I apologize is this is not strictly a microscopy question. A
} microscopist colleague/friend of mine is interested in purchasing a
} high quality speech recognition software. The individual is
} handicapped and does not full use of their hands and would be using
} the software for scientific writing (primarily describing microscopy
} findings). Although I favor the Macintosh system (since they would
} also use the computer for image processing and publishing), I am
} looking for the best software to assist this individual. Any
} recommendations would be appreciated. Many thanks.
}
} JB
}
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}



From daemon Fri Sep 20 01:38:45 2002



From: =?ISO-8859-2?Q?Old=F8ich_Benada?= :      benada-at-biomed.cas.cz
Date: Fri, 20 Sep 2002 08:30:15 +0200
Subject: Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,
C2 apertures in our CM12 are heavily contaminated. Does anybody could suggest how
to clean them? Heating in molybdenum boat does not work.
Thanks for any responses. O. Benada



+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-41062399
Fax: +420-2-41062347
WEB: http://www.biomed.cas.cz/mbu/lem113/lem.htm



From daemon Fri Sep 20 02:56:56 2002



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 20 Sep 2002 08:49:05 +0100
Subject: Re: SEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Debby
I have used Kodak TMax 100 in 35mm and 120 (6x7cm) formats for many
years and can thoroughly recommend it.
In 6x7 cm images the film can record all of the data available from
the SEM record monitor. Images are suitable for enlargement to at
least 16x20", often 20x30" and at these mags film grain is much less
important than image noise. I doubt if you would gain very much by
using 5"x4", and the negatives would be much more trouble to process.
However, there is significant loss of image quality when 35mm format
is used. This may be unimportant for images of the size required in
scientific publications, but degrades the quality of exhibition
images.
Chris

} Debby,
}
} I would certainly consider Kodak Tech Pan 4415. Speed is dependent
on
} development but will be slower than T-Max, around 25-32 and grain
structure
} is very fine.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu
}
}
}
}
} } Listers,
} } I am looking for a very fine grained film to use for capture of
SEM
} } images. We want to use this film in place of the Polaroid Type
55P/N film.
} } Kodak recommends the Tmax 100 4x5 sheet film.
} } I would appreciate other recommendations or confirmation that
this would
} } be the film of choice.
} }
} } Thanks,
} } Debby
} }
} } Debby Sherman, Manager Phone: 765-494-6666
} } Life Science Microscopy Facility FAX: 765-494-5896
} } Purdue University E-mail: dsherman-at-purdue.edu
} } S-052 Whistler Building
} } West Lafayette, IN 47907
}
}



From daemon Fri Sep 20 04:05:13 2002



From: Ian MacLaren :      maclaren-at-tu-darmstadt.de
Date: Fri, 20 Sep 2002 10:57:06 +0200
Subject: TEM heating/deformation stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
We are interested in purchasing a heating/tensile stage for our Phillips
CM20 (Supertwin lens). We are intending to use this for studying
deformation in thin films.

I would appreciate information and approximate prices from manufacturers.

I would also appreciate comments from users of such stages.

Thanks in advance

--
Ian MacLaren
Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany
ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/



From daemon Fri Sep 20 05:24:19 2002



From: Dr Adam Papworth :      ajp5-at-liverpool.ac.uk
Date: Fri, 20 Sep 2002 11:21:53 +0100
Subject: VG HB601 UX problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear All VG Users,

Here at Liverpool we have a VG HB 601 UX, which has developed a major
fault within the way the probe is scanned. However, before the scanning
fault developed, there were other intermittent faults and I feel that
all these faults are connected.
The first intermittent fault was an error message informing;

18:09:42 L9
Column Crate Interface
Reset
followed by
18:09:42 L9
Column Crate Interface
18:09:42 L9
Column Crate Interface
+15V Failure
This failure resulted in the stage translates not working.
By rebooting both computers the fault would be cleared, this fault would
happen once a week and then it progressed to once a day.

After rebooting sometimes the scanning would not be correct, i.e.
instead of an image appearing we would just get parallel lines or the
image of the VOA would be twisted and distorted.
The faults have now progressed to a point of not being able to form an
image at all in image mode, in Lo Mag Mode the image sometimes forms,
however we can see it flick on and off without touching the console. In
diffraction mode, sometimes we can see the objective aperture but
usually no image of the ADF detector
We have now lost all power to the middle and right hand side of the
control panel and the console will not boot up anymore.

All help and ideas on where to start in rectifying these faults would be
greatly welcome.

Adam

Please could all replies be to my e-mail address: ajp5-at-liv.ac.uk


--
Dr Adam Papworth,
Senior Experimental Officer,
Department of Engineering,
The University of Liverpool,
Liverpool,
L69 3GH, UK.

Phone
(Work) 0151 794 4672
(Mobile) 0151 794 7587
07970 24 7587
(Home) 0151 283 8596
(FAX) 0151 794 4675
e-mail ajp5-at-liv.ac.uk




From daemon Fri Sep 20 06:54:04 2002



From: John Peters :      edwhard465tom-at-estpak.ee
Date: Fri, 20 Sep 2002 04:44:22 -1900
Subject: Did You Get It yet 7950

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


HOW TO 'PICK UP' BEAUTIFUL WOMEN by John Eagan,
THE BOOK THAT IS GETTING NOTICED!

PENTHOUSE MAGAZINE promoted the author, John Eagan,
as a "Miracle Worker."

Penthouse Magazine's July '99 cover says "Getting
Beautiful Girls - Self Styled Pick Up Guru Shares His
'Super Techniques.'" According to Penthouse Magazine, John
Eagan had to take out an individual named "Bill."
Penthouse described it as follows -(page 132) "John's got
his work cut out for him with Bill who is in his 40's,
balding, wears glasses and sports an ill advised goatee."
Yet Penthouse goes on to say that Bill, using John Eagan's
techniques, (page 133) "obtained 3 women's phone numbers
that night."

On November 20th '99, John Eagan - author of the book
"How To Pick Up Beautiful Women" - appeared as a guest
on the Howard Stern Radio Show, CBS TV. According to
Howard Stern, John Eagan was asked to go out with the E!
crew and their hidden cameras to get dates for some of
their interns. Howard said the interns were the ugliest
guys he could find. John Eagan had to coach the interns
with his techniques by speaking into the interns ear
pieces. Using John Eagan's techniques, these interns got
dates with beautiful girls. Howard said "This guy is no
bull, I've seen him in action. He's the real deal."

Call for information call: 1-800-4-SECRET
OR to receive more info by email click on link below.
Mailto:kirkp4618-at-excite.com?Subject=tellmemore

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Women, Secrets Every Man Should Know" by John Eagan was
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Men's Fitness Magazine decided to take on the
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less than NUMBER 1.

The author, John Eagan has acquired two college degrees
writes a popular sex and dating column for "Muslemag
International" and hosts his own radio show. He has
interviewed over 2000 beautiful women and incorporates
that with the latest university studies on dating for
this powerful book. He has also appeared on many national
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Call for information call: 1-800-4-SECRET
OR to receive more info by email click on link below.
Mailto:kirkp4618-at-excite.com?Subject=tellmemore

} } HOWARD STERN said "This guy is no bull, he's the real
deal. I've seen him in action." "Wait until you see how
amazing this guy is."

} } GERALDO RIVERA was quoted as saying, "This book can
get men over their shyness."

} } ROLLING STONE MAG called John's techniques "genius."

} } DANNY BONADUCE said, "John Eagan is a genius, you
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} } SALLY JESSY RAPHAEL was quoted as calling the
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} } GORDON ELLIOTT told his audience, "Take this man's
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} } HARD COPY was quoted as saying, "This book can make
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like to read the Men's Fitness article for yourself and
the introduction to the book along with what other
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If you want a free copy of the Men's Fitness Magazine
article which rated this book number 1, give us a call.

Call for information: 1-800-4-SECRET
OR
Mailto:kirkp4618-at-excite.com?Subject=tellmemore

The Book, "How To 'Pick Up' Beautiful Women..."
by John Eagan can be ordered as follows:

PRICE: $14.95 plus $3.00 s/h (within the US).
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You can order this book with a VISA, MasterCard or American
Express by calling us at 1-800-4-SECRET and an operator
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If this email has reached you in error, we appologize.
Just click on the link below and you will be removed.
MailTo:donnyr6237-at-yahoo.com?Subject=remove-me



From daemon Fri Sep 20 07:22:17 2002



From: robert.fowler-at-tdktca.com
Date: Fri, 20 Sep 2002 08:19:35 -0400
Subject: Re: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Maybe this is the location you want?
















Dia-Tech Corporation
6408 Clinton Highway, Powell, TN 37849
(865) 938-2720









Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



Debby Sherman
{dsherman-at-purd To: "message to: MSA list"
ue.edu} {microscopy-at-sparc5.microscopy.com}
cc:
09/19/2002 Subject: OS staining of rubber
01:01 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
We, being non-material people, need some help with a material sample.
The sample is a high impact polystyrene (HIPS) that contains butadiene
rubber moieties. We need to be able to identify the location of these
rubber moieties and understand that they will stain upon exposure to
osmium.
Does anyone have any experience with this type of sample. Would it be
advisable to stain by floating thin sections (on grids) on liquid osmium or
by using osmium vapors? What type of grids would be most desirable to
minimize corrosion by the osmium?

Also, we have a diamond knife made by Diatech in Tennessee. I need to
contact them to obtain information about this knife. Does anyone have a
phone number or other contact information?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907







From daemon Fri Sep 20 07:35:02 2002



From: zaluzec-at-microscopy.com
Date: Fri, 20 Sep 2002 07:24:34 -0500
Subject: Re: Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Plasma Cleaning is another solution which I have used
on Condensor Apertures. Make sure you use the most
reactive species possible... i.e. an Oxygen Rich Plasma.

If they are badly contaminated you may have to go
to the high power Plasma Ashing regime.

However, if they are very heavily contaminated even that may
not work since some residue may remain. In this case you will
just have to replace them.


Disclaimer: My Employer ANL holds the Patent on Plasma Cleaning
Technology for application to Electron Microscopy Specimens &
In-situ Microscope applications. There are also commerical
organizations which may be able to help you such as SPI, XEI, South
Bay Tech and Fischione, all of whom are licensee's of the ANL Patent.
Links to their respective WWW pages are on the WWW page below.

http://www.amc.anl.gov/Docs/ANL/TechTrans/PlasmaCleaning.html



Cheers...

Nestor
Your Friendly Neighborhood SysOp



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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Sep 20 07:47:23 2002



From: zaluzec-at-microscopy.com
Date: Fri, 20 Sep 2002 07:32:34 -0500
Subject: Re: VG HB601 UX problems

Contents Retrieved from Microscopy Listserver Archives
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Adam

Check the 10/20 volt power supplies in the electronics racks. The
symptoms you describe I have seen many times.

Look both at the fuses as well as the thermal sensors on each
of the power supplies. Both of which are easily checked by measuring
the resistance with an Ohm meter. Both of which have failed on my system
at various times. I would tend to suspect the thermal sensor.

These units are located on my system (603Z) on the back of the Electronic
Power Supply Rack, underneath the Objective Power Supply, on the
601 they might be elsewhere.

You can tell immediately if you have power to these units by looking for the
"red" LED's on each of the units. They should be lit. If off, then power down
the machine and pull the suspect unit and start checking each one. Start
with the higher voltage supplies (20V) since they also supply power to a few
of the lower voltage (10V) supplies.

Cheers....

Nestor
Your Friendly Neighborhood SysOp.






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Sep 20 07:59:43 2002



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 20 Sep 2002 08:52:11 -0400
Subject: Re: SEM film

Contents Retrieved from Microscopy Listserver Archives
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I have always felt that Polaroid P/N 55 was a very good choice for SEM
image capture. While the positive print is nothing to write home about,
the negative is of very high quality (in terms of sharpness, line to line
resolution, and so on - granted it is rather low contrast). When printed
on the old grade 4 Ilfobrom paper it gave excellent prints. I am assuming
you are using fresh film, of course. A number of years ago I experimented
with switching from Type 55 to conventional film, but my users wouldn't use
it, and in the end I gave up on it myself. The fiddle of loading film
holders and developing and printing wasn't worth the cost saving to most
users. In the meantime they had working copies of all their images in the
Polaroid positives. Not only that, but the conventional process required
MUCH more support from me and my staff (how many of today's students knows
the first thing about conventional B&W photography?)

The resolution and graininess of large format films for SEM images is
usually quite unimportant, because the limiting factor in the image quality
is the screen of the CRT. Even a relatively coarse-grain film like the old
Kodak Tri-X can resolve better than 50 lines/mm (fine-grain films can
resolve over 300 lines/mm). To generate this amount of information, on a
10cm^2 image, would require 10,000x10,000 pixels. I don't know of a CRT
that can resolve that!

Of course, other labs' priorities may not be that same as mine, and there
may be excellent reasons to switch from Polaroid, but my choice was to not
follow that route.

Tony.

At 02:55 PM 9/19/2002 -0500, Debby Sherman wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Fri Sep 20 08:13:16 2002



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Fri, 20 Sep 2002 09:12:24 -0400
Subject: Re: hf antidote gel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Kai;

We routinely use concentrated HF in our lab. and keep calcium gluconate gel
for first treatment. However, be aware that it is a first treatment after a
water rinse and you should get to a hospital ASAP or risk losing some tissue
or worse.

Here are the details:

Manufactured by:

Calium Gluconate Gel

Calgonate Corp.
Rhode Island, USA

Distributed by:

Pharmascience Inc.
Montreal, Canada

You may call 1-800-363-8805 for info.

Regards,

Peter Tomic
Anadigics, Inc.
Warren, New Jersey
The United States of America



-----Original Message-----
} From: Kai Lorcharoensery [mailto:kai-at-lehigh.edu]
Sent: Thursday, September 19, 2002 2:36 PM
To: microscopy-at-sparc5.microscopy.com


The gel is calcium gluconate. You can buy a few tubes from Fisher (HF
antidote gel) or Pharmascience.

Kai Lorcharoensery
Materials Science & Engineering
Lehigh University
5 E. Packer Ave.
Bethlehem, PA 18015

"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com wrote:

} Hi Folks I am curious if any of you using HF are utilizing the hf antidote
gel. If you are where can I find some in US. Thank You
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}



From daemon Fri Sep 20 08:34:46 2002



From: Greg Mulhollan :      mulhollan-at-extremedevices.com
Date: Fri, 20 Sep 2002 08:27:38 -0500
Subject: RE: SF6 safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Mick Thomas was inquiring about SF6 safety. Ah, that brings back such
fond memories. Back in my days at SLAC, we were using SF6 as the high
voltage insulating gas for our polarized electron gun. We spent a good
deal of time determining risk levels since the gun operated in a
relatively unventilated underground tunnel separated from the other
sections of the accelerator by large doors. Here are a few of the items
we determined:
1. SF6 is an asphyxiant and not a poison. In its pure form it is pretty
unreactive.
2. SF6 is quite heavy and will immediately sink to floor level if there
are in intervening flow barriers. You can consider SF6 as being trapped
somewhere if you think of the analogous amount of water being poured
from or contained in a vessel. In other words, if you have a big vessel
filled with SF6 and open the top, it will retain a good deal of the SF6
for quite some time and still contain very little oxygen!
3. Filling our gun injector room with one complete large bottle of SF6
resulted in only about one inch of an oxygen depleted layer at floor
level.
4. SF6 after having been in the presence of high voltage arcing, will
break down forming some pretty nasty fluorimers, but generally at low
concentrations.

In summary: SF6, due to its weight and (usual) inert qualities presents
little risk, except if it is trapped in something or is broken down to
its sub-components. Hope that helps.

Greg M.

} Fellow microscopists,
}
} I would be very grateful if someone could offer me advice on how to
} ensure that a microscopy room is set up to safely handle a leak of } SF6.
}
} Thank you very much for your time and help.
}
} Sincerely,
}
} Mick
}
} -------------------------------------------------
} Mick Thomas
} UHV-STEM Laboratory
} E-1 Clark Hall
} Cornell University
} Ithaca, NY 14853
}

Gregory Mulhollan, Ph.D.
Senior Scientist
Extreme Devices Inc.
3500 ComSouth Drive
Austin, TX 78744
(512)439-3512 voice
(512)439-3487 fax
mulhollan-at-extremedevices.com



From daemon Fri Sep 20 08:38:29 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 20 Sep 2002 09:24:11 -0400
Subject: Re: hf antidote gel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


}
} Hi Folks I am curious if any of you using HF are utilizing the hf antidote
} gel. If you are where can I find some in US. Thank You
}
} Robert Fowler
************************
I buy my HF from Aldrich Chemicals.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Sep 20 08:52:17 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 20 Sep 2002 08:45:54 -0500
Subject: SEM film summary

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I arrived at work today to find a number of responses to my earlier post
asking about film for SEM. I have listed them below without reference to
sender.
I also had a number of responses asking why I wanted to use film for
SEM. This is a good question since for 99% of our SEM work we do capture
digital images and they are adequate for most purposes. However, I guess I
am still from the old school. I still find film better than digital if you
want to enlarge images.
In this particular case, we are hoping to purchase a high resolution
FESEM. A goodly percentage (not all) of the samples will be very
small...things like viruses, liposomes, and various nanoparticles with sizes
ranging from 10nm up. Although the newest models of FESEM can resolve these
structures, even though we will also have to deal with cryo conditions as
most samples will be hydrated, there is still the problem of "empty
magnification" when taking the original image. This limit to the useful
magnification when we originally capture the image is the potential problem.
In many cases we envision that we may need to substantially enlarge
single particles to illustrate finer features to our audience. In this
case, using very fine grained film will give us more opportunity to increase
size without concern for pixelization or without having a computer decide
how to insert smaller pixels (as would happen if we just punch in a higher
resolution figure and let the computer interpret). In fact, it surprises me
that for a relatively small sum when considering the cost of the entire
instrument package, many people do not choose to have the option of using
film when purchasing a new high resolution instrument. You may never use it
but you may well wish to have the option sometime down the road.

Again, thanks to all who responded. I may get a few more responses as
the day goes on but wanted to get this off before it gets buried in my in
box. As to final choice of film....I plan to try a number of the suggested
ones and see what fits our needs.

Debby


==============

Tmax is a bit on the contrasty side. For wider range, I prefer
Ilford FP4+ (rated ISO125, shoot at ISO 100).

This film comes in sheets and rolls (120/220). You can
use cut film holders for 4x5 or use a 6x7cm or 6x9cm roll back for
use with the roll film. Horseman or Wista are the best brands
of roll film backs. These slide into the Graflok back where the
Polaroid back was. Remove the ground glass clam shell affair
by pressing the two chrome latches and slide the ground glass
off the photo unit.

Roll film approach is cheap and very easy to use. Not the same
size as 4x5 but you have the option.

==========

I would certainly consider Kodak Tech Pan 4415. Speed is dependent on
development but will be slower than T-Max, around 25-32 and grain structure
is very fine.

==========
I have used Tech pan 120 roll film in the past and found it to be a very
fine
grained film. Of course you don't get positives untill you develop it,
although
you wouldn't get positives with the Tmax 100 sheet film either.

=============
T-max 100 is excellent film which I have used in both 35mm as
well as 4"x5" formats and have been extremely satisfied with the
grain size. I'm not sure if it comes in quick load sheets which
would look just like the polaroid type 55 film and also uses the 545
holder but it probably does. Otherwise just get a few film holders.
They are not going to be around much longer, although people have
been saying this for years.
===========

I have used Kodak TMax 100 in 35mm and 120 (6x7cm) formats for many
years and can thoroughly recommend it.
In 6x7 cm images the film can record all of the data available from
the SEM record monitor. Images are suitable for enlargement to at
least 16x20", often 20x30" and at these mags film grain is much less
important than image noise. I doubt if you would gain very much by
using 5"x4", and the negatives would be much more trouble to process.
However, there is significant loss of image quality when 35mm format
is used. This may be unimportant for images of the size required in
scientific publications, but degrades the quality of exhibition
images.
=============

I used Agfa APX 100 in 120 rollfilm for SEM images. It exist too in 4x5".
So fine as the T-max and much cheapper. For normal use, I develop it with
Rodinal 1+25. For fine grain use, it's better to use Attomal-FF

=============


Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Fri Sep 20 09:22:34 2002



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 20 Sep 2002 10:15:22 -0500
Subject: Dia-Tech

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Robert Fowler wrote:
===========================================
Maybe this is the location you want?

Dia-Tech Corporation
6408 Clinton Highway, Powell, TN 37849
(865) 938-2720
===========================================
This is a non-working number and so far as I know, they are no longer in
busienss.

However, there are several firms that would be able to resharpen diamond
knives made by Dia-Tech including SPI, MicroStar, DDK, Drukker and others.

Chuck

PS: Remember that we are striving to be 100% paperless, therefore there
are no paper copies kept of this correspondence. Please be sure to always
reply by way of "reply" on your software so that the entire string of
correspondence can be kept in one place.
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================














From daemon Fri Sep 20 09:40:00 2002



From: Stephenson, Matthew :      stephenson-at-impactanalytical.com
Date: Fri, 20 Sep 2002 10:33:15 -0400
Subject: OS staining of rubber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Debby,
We routinely examine HIPS material in the TEM. Our method is to stain the
trimmed and faced sample block in liquid Osmium solution (2% aq) before
sectioning. Just place the block in a sealed bottle of solution, only
enough to cover is needed, and leave in your hood overnight. Remove in the
morning into a small bottle of tap water in the hood for 5 minutes or so,
then set the block out in the hood to air dry for a half hour or so. The
block should be much darker.
Room temperature section as you normally would. Make a careful approach,
because the stain will only penetrate for on the order of 10 sections -at-
100nm. 70nm to 90nm sections look very good.
HIPS stains very well with this method. Some HIPS samples will warp,
making approach more difficult, but usually only to an annoying rather than
catastrophic degree. If you have problems, please contact me off-list about
recalcitrant samples.
Sincerely,
Matt

Matthew K. Stephenson
Analytical Associate
Impact Analytical
1910 West Saint Andrews Road
Midland, MI 48640
(989) 832-5555 X506
stephenson-at-impactanalytical.com



-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Thursday, September 19, 2002 1:02 PM
To: message to: MSA list


Listers,
We, being non-material people, need some help with a material sample.
The sample is a high impact polystyrene (HIPS) that contains butadiene
rubber moieties. We need to be able to identify the location of these
rubber moieties and understand that they will stain upon exposure to osmium.
Does anyone have any experience with this type of sample. Would it be
advisable to stain by floating thin sections (on grids) on liquid osmium or
by using osmium vapors? What type of grids would be most desirable to
minimize corrosion by the osmium?

Also, we have a diamond knife made by Diatech in Tennessee. I need to
contact them to obtain information about this knife. Does anyone have a
phone number or other contact information?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Fri Sep 20 09:55:40 2002



From: Claude Grattepain :      claude.grattepain-at-thalesgroup.com
Date: Fri, 20 Sep 2002 17:22:08 +0200
Subject: SEM, EDX, interferences

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




Hello,
I am a beginner in the EDX field. Is there any free software which could
allow to list the possible energie interferences for a given matrix ?
thanks and regards

Claude GRATTEPAIN

Phone : +33 1 6933 91 13 / Fax : +33 1 69 33 07 40
Mobile : +33 6 84 104 123
mailto:claude.grattepain-at-thalesgroup.com
THALES RESEARCH & TECHNOLOGIES FRANCE
L'Orée de Corbeville - 91401 ORSAY - BP 56 - FRANCE
*****************************************************

The information contained in this e-mail/fax and any attachments are the
property of THALES and may be confidential. If you are not the intended
recipient, please notify us immediately, send this message back to us
and destroy it. You are hereby notified that any review, dissemination,
distribution, copying or otherwise use of this e-mail/fax is strictly
prohibited.




From daemon Fri Sep 20 11:34:32 2002



From: William Oxberry :      William_Oxberry-at-downstate.edu
Date: Fri, 20 Sep 2002 12:26:41 -0400
Subject: Re:Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
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Oldrich-

I just cleaned my condenser aperature using metal polish on a cotton swab.
The same I use to clean the wehnelt. It took care of the very heavy
contamination. I sonicated in acetone two changes for 5 min. each and its
now working fine. You cannot do this with thin foil aperatures (ie.
objective aps.)

Good Luck,

Bill




From daemon Fri Sep 20 11:40:12 2002



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Fri, 20 Sep 2002 09:34:21 -0700 (PDT)
Subject: Re: Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Oldrich:

Where I work, I have the luxury of a metallographic
lab available. To clean apertures, I simply use
diamond polishing compound and napped cloth. I use
the tip of my finger to move the aperture in the
diamond compound around on the cloth until the
contaminants are gone. It usually takes just a few
seconds. Follow up with ultrasonic cleaning and
rinsing.

I believe this method is far superior and easier than
baking in removing contaminants. Keep in mind there
is always the risk of catching a corner and folding
the aperture, rendering it useless, which hasn't
happened to me yet.

Stu Smalinskas
SKF NATC
Plymouth, Michigan


__________________________________________________
Do you Yahoo!?
New DSL Internet Access from SBC & Yahoo!
http://sbc.yahoo.com


From daemon Fri Sep 20 11:50:17 2002



From: Marvin H Stromer :      mstromer-at-iastate.edu
Date: Fri, 20 Sep 2002 11:43:59 -0500
Subject: Emeritus Membership

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


As described in the MSA Bylaws, article 4, section 3, paragraph e, I wish
to apply for emeritus membership in MSA. I have been a member since 1966,
have been President of the Local Affiliated Society in Iowa and will retire
from employment at Iowa State University on December 31, 2002. Please
contact me if you need additional information.

Sincerely,
Marvin H. Stromer




From daemon Fri Sep 20 11:51:54 2002



From: John W. Raffensperger, Jr. :      johnr-at-helwigcp.com
Date: Fri, 20 Sep 2002 11:42:43 -0500
Subject: Software: speech recognition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I don't know about specifics for the Mac, but have some Wintel specifics
and general information to offer:

The two "leading" packages for the PC are IBM ViaVoice and Dragon
NaturallySpeaking (made by ScanSoft).

Both have a line of products that very in level of sophistication. They
also both offer specialized versions for specific industries, including
Medical and Legal. I'm not sure if they have "scientific" modules or
not. The modules basically add specialized vocabulary.

There is a matrix of capabilities that are common to most voice
recognition software. One side of the matrix is the "training side",
the other is the "function side".

On the function side, there are basically two modes: Command mode, and
dictate mode. Dictate mode allows dictation (e.g. microscope
observations) into an application. Most of the PC based system can
integrate with the common software, as well as just provide "keyboard"
mimicking into any application that accepts text. The integration with
something like Word provides the ability to handle formatting as your
dictating. The command mode provides access to the Windows interface,
and control of applications and the windows environment. Most dictating
capable products include command mode, but you can find "command mode"
only navigators (probably not appropriate in this case).

On the training axis, there is a continuum. In a perfect world, a voice
recognition system could allow anyone to walk up and start using it with
100% accuracy. The problem here is that we don't all have a monotone
voice with perfect Midwest (pick your geographic area) diction. Not to
mention foreign languages and industry specific "jargon" and
terminology. There are programs that do try and do this, and they are
"speaker independent" systems. While these systems are getting better
as processing power and programming techniques get better and faster,
they tend not to be as robust and accurate as systems that provide some
level of training.

The speaker dependent systems will require varying degrees of training
before the system will work. This generally involved reading a
paragraph or two. From this sample paragraph(s), the system builds a
profile of how to recognize the voice. The more training the more
accurate (in general). The better systems will also provide methods for
integrating additional training as you work with the system. They
provide tools for correcting the errors, and track the errors and
corrections, and integrate them into the profile. The level of ease of
use and sophistication of this ongoing training is one of the things
that separate the quality of the packages. With a motion impaired user,
what method and mechanics a given system uses may have a high impact
(positive or negative) on how effective the system is long range.

Also in the "training" axis would be the ability to add vocabulary. In
a specialized application like microscopy, this would be very important.

I'd also add that technology is changing fast. Look at the revision
history of any product you're considering. If it seems static, I would
suggest that you do NOT consider this as "stable" (and hence a positive
thing) in this application. Programming techniques are changed rapidly,
and software that is slow to adapt will be "behind".

I realize that I didn't provide much specific help, but I hope the
background helps.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.

-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Tuesday, September 17, 2002 3:26 PM
To: Microscopy-at-sparc5.microscopy.com


I apologize is this is not strictly a microscopy question. A
microscopist colleague/friend of mine is interested in purchasing a
high quality speech recognition software. The individual is
handicapped and does not full use of their hands and would be using
the software for scientific writing (primarily describing microscopy
findings). Although I favor the Macintosh system (since they would
also use the computer for image processing and publishing), I am
looking for the best software to assist this individual. Any
recommendations would be appreciated. Many thanks.

JB

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################



From daemon Fri Sep 20 12:09:39 2002



From: gary.m.brown-at-exxonmobil.com
Date: Fri, 20 Sep 2002 12:02:38 -0500
Subject: Re: OS staining of rubber

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Debby,

Tobias is correct that RuO4 will stain such rubbers. Unfortunately, RuO4 is
not a selective stain the either the rubber or polystyrene components of
HIPS (stains both heavily). OsO4, on the other hand, will stain only the
unsaturated polybutadiene of HIPS. I think that you will find OsO4 much
more useful.

Regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



Tobias Baskin
{BaskinT-at-missou To: microscopy-at-sparc5.microscopy.com, Debby
ri.edu} Sherman {dsherman-at-purdue.edu}
cc:
Subject: Re: OS staining of rubber
09/19/02 03:16
PM





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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Debbie,
There is some literature suggesting that ruthenium tetroxide
is better than osmium for this kind of thing. There is a paper by
Trent, Scheinbeim, and Couchaman 1983 Macromols 16: 589-598 that is
very informative. My understanding is that it is usual to vapor
stain this kind of sample.
Hope this helps,
Tobias
}
}
}
} Listers,
} We, being non-material people, need some help with a material sample.
} The sample is a high impact polystyrene (HIPS) that contains butadiene
} rubber moieties. We need to be able to identify the location of these
} rubber moieties and understand that they will stain upon exposure to
osmium.
} Does anyone have any experience with this type of sample. Would it be
} advisable to stain by floating thin sections (on grids) on liquid osmium
or
} by using osmium vapors? What type of grids would be most desirable to
} minimize corrosion by the osmium?
}
} Also, we have a diamond knife made by Diatech in Tennessee. I need
to
} contact them to obtain information about this knife. Does anyone have a
} phone number or other contact information?
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} West Lafayette, IN 47907

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological
Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO
USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123







From daemon Fri Sep 20 12:15:41 2002



From: Stephenson, Matthew :      stephenson-at-impactanalytical.com
Date: Fri, 20 Sep 2002 13:09:43 -0400
Subject: Re: Os staining of rubber

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Debby,
We routinely examine HIPS material in the TEM. Our method is to stain the
trimmed and faced sample block in liquid Osmium solution (2% aq) before
sectioning. Just place the block in a sealed bottle of solution, only
enough to cover is needed, and leave in your hood overnight. Remove in the
morning into a small bottle of tap water in the hood for 5 minutes or so,
then set the block out in the hood to air dry for a half hour or so. The
block should be much darker.
Room temperature section as you normally would. Make a careful approach,
because the stain will only penetrate for on the order of 10 sections -at-
100nm. 70nm to 90nm sections look very good.
HIPS stains very well with this method. Some HIPS samples will warp,
making approach more difficult, but usually only to an annoying rather than
catastrophic degree. If you have problems, please contact me off-list about
recalcitrant samples.
Sincerely,
Matt

Matthew K. Stephenson
Analytical Associate
Impact Analytical
1910 West Saint Andrews Road
Midland, MI 48640
(989) 832-5555 X506
stephenson-at-impactanalytical.com




From daemon Fri Sep 20 12:58:04 2002



From: Rita Monahan-Earley :      rmonahan-at-caregroup.harvard.edu
Date: Fri, 20 Sep 2002 13:48:34 -0400
Subject: Metal "Boats"

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Dear Members of this valuable site,
I am interested in buying some LKB metal boats "Metal Collecting
Troughs" 4815B) to be used on glass knives.

We have a supply in our lab but need more.
If anyone has any leads on where I can order these, it would be much
appreciated.

I'm not adverse to buying pre-owned boats. If they have been used
gently.

Thank you,
Rita


From daemon Fri Sep 20 14:47:35 2002



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 20 Sep 2002 13:02:28 -0700
Subject: Re: SEM film summary

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Debby

I would completely agree with your point about high resolution film etc if
we are talking about TEM. In TEM, image forming electrons directly
interact with film. So, the quality of the film is a 'limitation factor'
there. In TEM even now the film has some advantages over the digital
camera. The film greatest advantage - as you mentioned in your message, is
ability to enlarge the image without serious pixelation.

In SEM, image forming electrons are detected by some sort of device
("detector"). So, at the output you have actually electrical signal, which
represents the properties of your sample. This electrical signal then
converted back into the image on the CRT. You also may project CRT image
on the film and have a "hard" copy. In such scenario, the image resolution
on the film dependent from the quality of the "projection system" and
actual size of the SEM probe. Because, signal already present in the
"electrical form" (analog or digital) there is no reason to convert it into
the image on the film: with modern image grabbers and high-quality printers
you may directly convert it into hard copy without intermediate (film)
step. In my point of view, this is fundamental difference between SEM and
TEM.

Another point, on the film, you could record about 100 shades of gray
(dynamic range is 10^2), modern digital devices will record 3-6K levels of
grey from the same sample. So, using film in SEM - you lost information,
because original signal was (and is) an electronic and then converted into
the image. When you directly print your image (digitally) you also loose
the dynamic range BUT: you'll still have the original digital image with
all that 6K shades of gray. Using film you automatically reduce the
dynamic range and, also, keep in mind that film is not linear: it records
image in the logarithmic scale... I am sorry... I don't see ANY advantages
of using film in SEM, but I DO see some advantages of using film in
TEM. Have a good day. Sergey



At 08:45 AM 9/20/02 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Fri Sep 20 15:11:07 2002



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 20 Sep 2002 14:04:18 -0400
Subject: seeking a job

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Hi All,
I was contacted by a Post-Doctoral fellow who is seeking a part-time
technical position in an EM lab in the NYC area. Her CV is available
upon direct request to her. Please respond to her directly, off-line.
Lee


ljiljan minwalla, PhD
email: minwal01-at-med.nyu.edu
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Sep 20 15:29:02 2002



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Fri, 20 Sep 2002 15:27:35 -0500
Subject: Re: SF6 safety

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Mick

Most States do not have specific requirements for SF6 handling as it is
classed as an inert gas. However it is an asphyxiant and will settle to
the floor replacing oxygen. Therefore any labs with a significant amount
of SF6 (T tank or more) should be well ventilated with a floor level vent
to the outside of the building. In general EM labs tend to be small and
not that well ventilated at ground level. In our case we have 3.5" pipe at
ground level, near the tank storage and HT generator, tied into a fume hood
plenum which has a fan capable of sufficient draught to remove any SF6 spilled.

It does have hazardous decomposition products either generated by electric
arcs (SF4, SOF2, SO2F2, OF2 and HF) or heating in the presence of steels
which act as a catalyst above 200degC (SF2, SF4, S2F10), however in the
quantities used, contained, in microscopy the potential for generation of
hazardous byproducts is small.

Regards

Alan

At 01:08 PM 9/19/2002 -0400, Mick Thomas wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Fri Sep 20 15:29:07 2002



From: Russell E. Cook :      recook-at-anl.gov
Date: Fri, 20 Sep 2002 15:23:09 -0500
Subject: RE: SF6 safety

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We had a large pit under the now-decommissioned HVEM here at Argonne. The
pit had a sensor to detect oxygen levels. If the oxygen level dropped
below a set point an exhaust fan was started and a warning horn sounded.
You shouldn't have to do that. As Greg has pointed out, you'd have to be
prone on the floor - face down - before you'd be asphyxiated by the typical
setup these days. Nevertheless, you might want to have a small exhaust fan
to the building exterior installed at floor level so that you can eliminate
as much SF6 as possible in the event of a serious leak. Talk to your
institutional safety people.

We also have 1 inch copper pipes installed in the microscope rooms to
exhaust/dump SF6 to the building exterior before servicing acceleration
chambers or HV tanks. The outlet of the pipes are about 3 to 4 feet above
the ground and have a perforated cap to keep out insects. We've also
posted warning signs near the outlets.

Russell E. Cook, Ph.D.
Electron Microscopy Center
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov
} -----------------------------------------------------------------------
} Date: Fri, 20 Sep 2002 08:27:38 -0500
} Subject: RE: SF6 safety
} From: Greg Mulhollan {mulhollan-at-extremedevices.com}
}
} Mick Thomas was inquiring about SF6 safety. Ah, that brings back such
} fond memories. Back in my days at SLAC, we were using SF6 as the high
} voltage insulating gas for our polarized electron gun. We spent a good
} deal of time determining risk levels since the gun operated in a
} relatively unventilated underground tunnel separated from the other
} sections of the accelerator by large doors. Here are a few of the items
} we determined:
} 1. SF6 is an asphyxiant and not a poison. In its pure form it is pretty
} unreactive.
} 2. SF6 is quite heavy and will immediately sink to floor level if there
} are in intervening flow barriers. You can consider SF6 as being trapped
} somewhere if you think of the analogous amount of water being poured
} from or contained in a vessel. In other words, if you have a big vessel
} filled with SF6 and open the top, it will retain a good deal of the SF6
} for quite some time and still contain very little oxygen!
} 3. Filling our gun injector room with one complete large bottle of SF6
} resulted in only about one inch of an oxygen depleted layer at floor
} level.
} 4. SF6 after having been in the presence of high voltage arcing, will
} break down forming some pretty nasty fluorimers, but generally at low
} concentrations.
}
} In summary: SF6, due to its weight and (usual) inert qualities presents
} little risk, except if it is trapped in something or is broken down to
} its sub-components. Hope that helps.
}
} Greg M.
}
} } Fellow microscopists,
} }
} } I would be very grateful if someone could offer me advice on how to
} } ensure that a microscopy room is set up to safely handle a leak of } SF6.
} }
} } Thank you very much for your time and help.
} }
} } Sincerely,
} }
} } Mick
} }
} } -------------------------------------------------
} } Mick Thomas
} } UHV-STEM Laboratory
} } E-1 Clark Hall
} } Cornell University
} } Ithaca, NY 14853
} }
}
} Gregory Mulhollan, Ph.D.
} Senior Scientist
} Extreme Devices Inc.
} 3500 ComSouth Drive
} Austin, TX 78744
} (512)439-3512 voice
} (512)439-3487 fax
} mulhollan-at-extremedevices.com
}




From daemon Fri Sep 20 15:44:54 2002



From: Steve Chapman :      protrain-at-emcourses.com
Date: Fri, 20 Sep 2002 21:22:39 +0100
Subject: Re: Heavily contaminated C2 apertures

Contents Retrieved from Microscopy Listserver Archives
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Hi

The standard way to clean molybdenum apertures is in a high vacuum coating
unit, heating the apertures to orange-red and holding them at that
temperature until the deposit(s) evaporate.

Heating in a sputter coater or in a flame does not work with molybdenum.
Heating to white heat is not a good idea as this often distorts the aperture
or, if you try to re use the cleaned apertures and clean them again grain
growth also tends to spoil the aperture shape.

If you are unable to clean the apertures by the first method described you
are better off discarding them; dirty apertures are not a good idea!

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com




From daemon Fri Sep 20 15:56:10 2002



From: simkin-at-egr.msu.edu
Date: Fri, 20 Sep 2002 16:47:00 -0400 (EDT)
Subject: Hard Disk Microscopy?

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Hi all,

I have had someone come to me asking about microscopic methods of reading
data on hard disk platters. Does anyone have experience along these lines,
or suggestions of who may have done this sort of thing? The question seems to
deal mainly with the persistence of the magnetic domains, and what sort of
'memory' the disk maintains during multiple writing operations.

Any suggestions would me greatly appreciated.

Ben Simkin (simkin-at-egr.msu.edu)
Michigan State University




From daemon Fri Sep 20 16:08:56 2002



From: saram-at-duke.edu
Date: Fri, 20 Sep 2002 17:00:09 -0400 (EDT)
Subject: Nikon shutter device for grabs

Contents Retrieved from Microscopy Listserver Archives
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I have uncovered a box ~4x5x2 in that looks like an exposure device for a
Nikon photomicroscope. It has selections for manual/auto, speed, ASA,
time, and over/under adjustments. It has a cord that fastens to the
camera with 8 pins around the periphery and 2 in the center. If anyone
can use it, I will mail it. Otherwise it goes into the dump.

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Sep 20 16:18:14 2002



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Fri, 20 Sep 2002 17:13:15 -0400
Subject: JEOL 2010F

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How many JEOL 2010Fs are there out there? If you have one I would
appreciate it if you would drop me a line directly and not to the list.
Thanks.

--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42! 16' 48" Long. 83! 43' 48"




From daemon Fri Sep 20 16:41:16 2002



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Fri, 20 Sep 2002 17:34:27 -0400
Subject: Re: Heavily contaminated C2 apertures

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Oldrich,

If the apertures are from a Philips TEM, they are most likely made of
Platinum. You don't heat these in vacuum as you do the Mo
apertures. These can be cleaned by heating them in a Pt basket over a
bunsen burner in air. The oxygen in air won't oxidize the Pt, but will
remove the hydrocarbon contamination quite effectively.

If you look in your CM12 owners book, Philips should have a section on
maintenance where they describe the procedure.

Cheers,
Henk Colijn


At 08:30 AM 9/20/2002 +0200, =?ISO-8859-2?Q?Old=F8ich_Benada?= wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Fri Sep 20 17:13:59 2002



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 20 Sep 2002 17:07:24 -0500
Subject: Re: SEM film & M&M2003

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Ann and others,
I am glad that you have found the MSA Facility Management series useful.
Unfortunately it may be held less often in the future. Due to the large
number of annually repeating sessions that limit addition of new sessions,
it looks like it will be relegated to every other year starting immediately
although there is no guarantee that it will be continued in the future. At
least there will be no support yearly so speakers will have to be gleaned
from those already going to the meetings. This is usually the case for most
anyway but has not been the case for all.
We will try to limp along if we can find appropriate facilitators
already going to the meeting and a room to hold the session. Hopefully we
will still get funding for the audio-visual required.
In any case let's be optimistic and plan on a session at M&M2003. With
that, I have a few ideas as a topic but, since this is meant to be relative
to all you lab managers out there, what would you like to have
presented/discussed at the next session? Please let me know with
suggestions for facilitators as well.

Debby



On 9/20/02 11:32 AM, "Lehman, Ann" {Ann.Lehman-at-trincoll.edu} wrote:

} Hi Debbie,
}
} Many moons ago (in the 80's) I regularly used Kodak Ektapan sheet film in
} 4x5 carriers inserted into the Polaroid holder, and got good results. Tech
} Pan sounds promising though.
}
} I can appreciate your desire to get good negatives, as I am struggling with
} an intro SEM course in which my students are only exposed to digital imaging
} (no pun intended). I feel I am short-changing them.
}
} Let us know what you learn!!
}
} And thanks again for organizing the MSA facility management series.
}
} cheers,
} Ann
}
} ###################
} Ann Hein Lehman
} Assistant Director, EM Facility
} Trinity College - LSC314
} 300 Summit Street
} Hartford CT 06106
} v. 860-297-4289
} f. 860-297-2538
} e. ann.lehman-at-trincoll.edu
} w. http://www.trincoll.edu/~alehman/
} Facility beta: http://www.trincoll.edu/~alehman/EM_Facility.htm
}



From daemon Fri Sep 20 17:14:01 2002



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 20 Sep 2002 15:07:02 -0700
Subject: Re: SEM film summary

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Dear Debby,
The limitation of resolution from an SEM image is not the fine grain of the
film, but the line resolution of the SEM and photo CRT and the ability of
the photo CRT and camera to resolve those lines. While you can enlarge a
TEM image ten times because the image is a real image and the film can still
resolve features at that enlargement, the SEM image will start to show you
the lines in the SEM CRT above about five times enlargement. If you do not
see the lines, then your SEM CRT is not focussing even that well and your
image will be degraded further. Fine film won't help that. The highest
resolution SEM photo CRT I have seen is 2500 lines and so that is the most
lines that the SEM will put out. There is no reason that a 2500 line image
from the film camera is going to be any better or sharper or higher
resolution than a 2500 line digital image. The only choice is the output
media and, with glossy photo paper, an high-resolution inkjet printer will
give you a two minute 8 X 10 print. There is nothing magic about film or
inferior about digital imaging.
At 08:45 AM 09/20/2002 -0500, you wrote:

} Listers,
} I arrived at work today to find a number of responses to my earlier post
} asking about film for SEM. I have listed them below without reference to
} sender.
} I also had a number of responses asking why I wanted to use film for
} SEM. This is a good question since for 99% of our SEM work we do capture
} digital images and they are adequate for most purposes. However, I guess I
} am still from the old school. I still find film better than digital if you
} want to enlarge images.
} In this particular case, we are hoping to purchase a high resolution
} FESEM. A goodly percentage (not all) of the samples will be very
} small...things like viruses, liposomes, and various nanoparticles with sizes
} ranging from 10nm up. Although the newest models of FESEM can resolve these
} structures, even though we will also have to deal with cryo conditions as
} most samples will be hydrated, there is still the problem of "empty
} magnification" when taking the original image. This limit to the useful
} magnification when we originally capture the image is the potential problem.
} In many cases we envision that we may need to substantially enlarge
} single particles to illustrate finer features to our audience. In this
} case, using very fine grained film will give us more opportunity to increase
} size without concern for pixelization or without having a computer decide
} how to insert smaller pixels (as would happen if we just punch in a higher
} resolution figure and let the computer interpret). In fact, it surprises me
} that for a relatively small sum when considering the cost of the entire
} instrument package, many people do not choose to have the option of using
} film when purchasing a new high resolution instrument. You may never use it
} but you may well wish to have the option sometime down the road.
}
} Again, thanks to all who responded. I may get a few more responses as
} the day goes on but wanted to get this off before it gets buried in my in
} box. As to final choice of film....I plan to try a number of the suggested
} ones and see what fits our needs.
}
} Debby
}
..snip

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Sep 20 17:44:24 2002



From: saram-at-duke.edu
Date: Fri, 20 Sep 2002 19:44:03 -0400 (EDT)
Subject: Shutter control

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Several years ago (4-5) we looked at this issue in regards to handling
rodent filth & concerns about hantavirus, etc. From the literature search
we found the following (which may have changed) when looking at latex vs.
nitrile gloves:

1. No known allergic reactions to nitrile.
2. Nitrile gloves had smaller pores than latex gloves.
3. Nitrile gloves had a lower failure rate during testing than latex gloves.

We switched to nitrile gloves for "filth" work.

These are my own
comments & not of my employer.
David A. Foran, chemist
Food and Drug Administration
Kansas City Elemental Analysis Lab
DFORAN-at-ORA.FDA.GOV
913-752-2170
913-752-2727 (voice mail)
913-752-2151 (fax)


-----Original Message-----
} From: "Frida.Maiers-at-co.hennepin.mn.us"-at-sparc5.microscopy.com
[mailto:"Frida.Maiers-at-co.hennepin.mn.us"-at-sparc5.microscopy.com]
Sent: Wednesday, September 18, 2002 1:30 PM
To: microscopy-at-sparc5.microscopy.com


The shutter control has found a home.

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Sep 20 20:09:26 2002



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 20 Sep 2002 21:10:40 -0400
Subject: Re: SEM film summary

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Mary,

It gets worse than that : c.a. 1985 when working on a SEM in a colleagues lab I
noticed that the horizontal scan lines that were normally distinguishable at
certain faster scan settings on my instrument were not visible on his (same mfg.,
same scan module, same recording CRT, different chamber and spectrometer setup).
When I inquired, he told me that the mfg.'s service rep had been there only the
day before and completely optimized everything, including readjusting the
brightness and contrast settings on the recording CRT.

After a little investigation I found that the tech had decreased the brightness
settings and compensated by opening the photo transfer lens iris from f-8 to
f-5.6. I had already learned from experience that an iris opening this wide led
to a a defocused image of the CRT spot on the film which could not be corrected
by any adjustment of the mechanical focus.

When asked why he had intentionally defocused the image, the tech responded that
the faintly visible scan lines on fast frame rates annoyed some customers so he
made them happy by effectively blurring the image so that the raster lines ran
together!!! (Names withheld to protect the guilty)

Part of the point of this story is that the vertical resolution on a SEM may be
limited by the number of lines in the raster pattern ra