Buehler has high speed (and slow speed) cutting machines that can be equipped with diamond wheels for cutting very hard materials. Look at http://www.buehlerltd.com/productinfo/precision_saws/Isomet5000.pdf
Disclaimer: I have no commercial interest in Buehler products. -- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
I received the following email from a friend in DC and don't know how to help her because I hopelessly burdened with expertise only in high end systems. My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, but any suggestions are appreciated. ----------------- HELP!!!! All Katie wants for Christmas is a good microscope. She wants to be able to really see things and does not want a "kid" microscope. I need something with good optics but not expensive. Used is fine. Any ideas, brand names, places to look? Thanks. ------------------
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
================================================= On Wednesday, November 27, 2002, at 06:20 AM, John Shields wrote: } I have several people that wish to look at possible metal uptake in } bacteria } and would prefer to enbed them in either Spurr's or Epon, however there } might be a problem with trace metals in these resins. } Questions are: } 1. Is this a real concern? and } 2. Would another resin be more suitable? } I'm trying to avoid cryo here. } Thanks
Dear John & listers,
With EDX of sections the sensitivity is at best about 0.05--0.1wt% so trace metals are unlikely to be a problem. Chlorine is detectable in Spurrs but probably won't interfere with the elements of interest. I have analysed metals in microalgae, and we did have difficulty seeing significant levels in the cells in resin sections, and found we had to go to cryosections. The implication is that a lot of the metals were lost during processing, so one has to be careful with the procedure adopted. There were also insoluble granules in the cells that appear to have fallen out from the thin resin sections. If it's at all possible, it would be a good idea to do a chemical analysis of a batch following metal uptake to check that the metals are indeed incorporated into (or on) the cells, so that you have some idea of what levels to expect and whether you might have lost some during processing.
With regards, Thor
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Dr Thor Bostrom Acting Director, Analytical EM Facility, Faculty of Science, Queensland University of Technology (QUT) GPO Box 2434, Brisbane, QLD 4001, Australia Ph: +61 7 3864-2351 FAX: +61 7 3864-5100 http://www.sci.qut.edu.au/aemf/ =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (vladig-at-tht.net) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, December 2, 2002 at 16:09:33 ---------------------------------------------------------------------------
Email: vladig-at-tht.net Name: Vladimir Igoshev
Organization: RIM
Education: Graduate College
Location: City, State, Country
Question: Dear All,
Does anybody know (can share) which etchant should be used for Electroless Nickel plating?
I had this dilemma a few years ago. The solution is not a microscope (expensive and not portable) but a good wide field hand lense. She can examine things and carry the hand lense around. It is perfect for the beginner.
You can visit {http://www.edmundoptics.com} and look at the magnifiers. For $50 she can get a 12x magnifier.
On Monday, December 2, 2002, at 02:03 PM, Michael Cammer wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I received the following email from a friend in DC and don't know how } to help her because I hopelessly burdened with expertise only in high } end systems. } My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, } but any suggestions are appreciated. } ----------------- } HELP!!!! All Katie wants for Christmas is a good microscope. She wants } to } be able to really see things and does not want a "kid" microscope. I } need } something with good optics but not expensive. Used is fine. Any ideas, } brand names, places to look? Thanks. } ------------------ } } } } ____________________________________________________________________________ } Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of } Med. } Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY } 10461 } (718) 430-2890 Fax: 430-8996 URL: } http://www.aecom.yu.edu/aif/ } } } Dr. Gordon Nord Senior Scientist Environmental Sciences Laboratory Brooklyn College Brooklyn NY 11210
If you really looking for microscope, the Russian microscopes may be a solution. I do know that LOMO is manufactured and distributed in US very cheap decent optical microscopes from very basic to serious models. Those microscopes are utilized old-fashion Carl-Zeiss German tradition, so they are really good if you not looking for sophistication. You may try the following link http://www.lomoplc.com/index2.html but I am pretty sure you may find more.
Best wishes, Sergey.
At 08:26 PM 12/2/2002, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Can anyone suggest a SAFE procedure for removing oil deposits from the surface of the ATW2 (light element) window on my Oxford detector? While we're at it, does anyone know of a process for cleaning/polishing the bottom of the inside of the dewar on the same detector. I looked inside and it appears to have a layer of deposits (dirt?) on the bottom. Might this be contributing to high LN2 consumption? TIA and MC and HNY. Bill Roberts
This communication is for use by the intended recipient and contains information that may be privileged, confidential or copyrighted under applicable law. If you are not the intended recipient, you are hereby formally notified that any use, copying or distribution of this e-mail, in whole or in part, is strictly prohibited. Please notify the sender by return e-mail and delete this e-mail from your system. Unless explicitly and conspicuously designated as "E-Contract Intended", this e-mail does not constitute a contract offer, a contract amendment, or an acceptance of a contract offer. This e-mail does not constitute a consent to the use of sender's contact information for direct marketing purposes or for transfers of data to third parties.
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Many of you sent excellent suggestions for kids' microscopes. Thanks! We'll let you know what they pick, and some of the suggestions look good for my kids too. Thanks!
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
The New York Microscopical Society has available to members two microscopes which are of very good quality and quite inexpensive.
We have a 20X dissecting microscope at $90 (members price $ 55) and a Compound microscope with 4X, 10X & 40X Society thread objectives and a built in light source at $225 (members price $155).
These are the same microscopes we use for our classes for children.
Don ----- Original Message ----- } From: "Gordon Nord" {gnord-at-mindspring.com} To: "Michael Cammer" {cammer-at-aecom.yu.edu} Cc: "Microscopy List" {Microscopy-at-sparc5.microscopy.com} Sent: Monday, December 02, 2002 11:26 PM
New York Microscopical Society
30 North Mountain Avenue
Montclair, NJ 07042
Bernard Friedman
Memorial Workshop
Digital Image Capture and Management in Microscopy
May 9, 2003
A course on Digital imaging in light microscopy which will cover the following topics:
Optical Limitations in Light Microscopy...Photographic Imaging Strategies... Digital Imaging Strategies...Selection of Digital Capture (Camera vs. Scanner)...
Image Processing of Captured Images...Image File Formats...Printing Images... Color Management Systems...Database Management Software...Presentation Software for Oral Reports...Website Performance...Integration of Image Data with Sample Information, Calibration, Other Data & Reports...Acrobat and html Software for Written Reports and Archives...Examples of Efficient, Low Cost Image Handling Systems...
Examples of Electronic Microscopy Reports and Databases
The course instructors are Mary and John McCann of McCann Imaging.
} I received the following email from a friend in DC and don't know how to } help her because I hopelessly burdened with expertise only in high end } systems. } My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, but } any suggestions are appreciated. } ----------------- } HELP!!!! All Katie wants for Christmas is a good microscope. She wants to } be able to really see things and does not want a "kid" microscope. I need } something with good optics but not expensive. Used is fine. Any ideas, } brand names, places to look? Thanks. } ------------------ Michael Cammer
Michael -
First, your friend should look at the microscope-buying advice on the MICRO website. The first decision is type; dissecting vs. compound - both have advantages. Sources are suggested on the website, but there isn't much shopping time; my personal favorite place for good onscreen advice and fair prices is www.microscopeworld.com. I'd avoid eBay for a first scope; a used one may have faults, and it's likely to be too complex as well. Both will frustrate a youngster.
Caroline
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
Try Acton Technologies, Pennsylvania, USA for a Ni etch, http://www.actontech.com/elec1.htm.
I've used their materials and they generally work as advertised. I have no interest in the company other than as a user. They send me no holiday gifts.
Regards,
Peter Tomic Anadigics,, Inc. Warren, New Jersey USA
-----Original Message----- } From: vladig-at-tht.net [mailto:vladig-at-tht.net] Sent: Monday, December 02, 2002 8:06 PM To: Microscopy-at-sparc5.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (vladig-at-tht.net) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, December 2, 2002 at 16:09:33 ---------------------------------------------------------------------------
Email: vladig-at-tht.net Name: Vladimir Igoshev
Organization: RIM
Education: Graduate College
Location: City, State, Country
Question: Dear All,
Does anybody know (can share) which etchant should be used for Electroless Nickel plating?
I have found that the things middle schoolers like to look at can be best observed with a good stereo microscope. A hand lens is great, but a stereo microscope with a light will be cooler to 5th grader.
In my town, we have several used scientific equipment dealers that peddle pretty decent used scopes for a reasonable price - better value for the money buying used Bausch and Lomb, Olympus or Nikon than a new cheap brand. It may take some hunting around to find these dealers. Ebay is another alternative. Make sure that the unit is a stereo microscope with two eyepieces.
Good luck. -- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
Dear Vladimir, My Vander Voort mentions two solutions for electroless nickel: Formula 1: 45 g nickel chloride 11 g sodium hypophosphite 100 g sodium citrate 50 g ammonium chloride 1000 ml. water pH 8.5-9, use a 194-212 °F. Plating rate: 0.015 mm/h
Formula 2: 37.3 g nickelous sulfate 26.4 g sodium hypophosphite 15.9 g sodium acetate 5-6 drops sulfuric acid 1000 ml. water Use at 180-190 °F, plating rate 0.01 mm/h
I hope this helps.
----- Original Message ----- } From: "by way of MicroscopyListserver" {vladig-at-tht.net} To: {Microscopy-at-sparc5.microscopy.com} Sent: Monday, December 02, 2002 5:06 PM
Two other sources similar to Edmund Scientific are:
Both are primarily aimed at school supply, but also do retail sales.
They have a full range of instruments from simple hand lenses, portable hand held "field microscopes" and beginning through "advanced" compound microscopes. Some of the more advanced scopes include a photo tube. They stop short of the real high end (Nikon, Olympus, etc.)
John W. Raffensperger, Jr. IS Manager Helwig Carbon Products, Inc.
-----Original Message----- } From: Michael Cammer [mailto:cammer-at-aecom.yu.edu] Sent: Monday, December 02, 2002 1:04 PM To: Microscopy-at-sparc5.microscopy.com
I received the following email from a friend in DC and don't know how to
help her because I hopelessly burdened with expertise only in high end systems. My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, but any suggestions are appreciated. ----------------- HELP!!!! All Katie wants for Christmas is a good microscope. She wants to be able to really see things and does not want a "kid" microscope. I need something with good optics but not expensive. Used is fine. Any ideas, brand names, places to look? Thanks. ------------------
________________________________________________________________________ ____ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
I recently collected a lot of images at a single sitting, each image seemingly better than the previous one, definitely on a roll. However, the next day I laboriously had to review a lot of images of similar data and choose the best ones.
What software are people using to browse through a lot of images, as thumbnails, easily change their names, perhaps add annotations, move selected ones into new folders and create a catalogue. It should also be able to recognize and load confocal image stacks.
Thank you Judy
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 8Queen 30 Bond St. Toronto, ON M5B 1W8 Canada
I am afraid that the given formula are for preparing the plating solution. The etchant that I tried consists of: 1 part of water, 1 part of acetic acid, and 2 parts of nitric acid. All are in volume ratio. This etchant works very fast. Dip a few seconds and see the result before proceeding. It reveals grain boundaries and striations of phosphorus-depleting layers. A word of caution, it can attacks the substrate violently such that you get a good coating microstructure but not the substrate.
In their book, Rostoker & Dvorak used 5 gram of CrO3 in 100 ml of water as a solution for electrolytic etching. This one reveals both P-striations and Ni3P (if present).
The most important thing is safety. Both HNO3 and CrO3 are strong oxidizer. CrO3 will turn to acid upon dissolving, and it is carcinogen. Please read MSDS before using them.
Kai Lorcharoensery Materials Science & Engineering Lehigh University Bethlehem, PA
Mary Mager wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Vladimir, } My Vander Voort mentions two solutions for electroless nickel: } Formula 1: } 45 g nickel chloride } 11 g sodium hypophosphite } 100 g sodium citrate } 50 g ammonium chloride } 1000 ml. water } pH 8.5-9, use a 194-212 °F. Plating rate: 0.015 mm/h } } Formula 2: } 37.3 g nickelous sulfate } 26.4 g sodium hypophosphite } 15.9 g sodium acetate } 5-6 drops sulfuric acid } 1000 ml. water } Use at 180-190 °F, plating rate 0.01 mm/h } } I hope this helps. } } ----- Original Message ----- } } } From: "by way of MicroscopyListserver" {vladig-at-tht.net} } } To: {Microscopy-at-sparc5.microscopy.com} } Sent: Monday, December 02, 2002 5:06 PM } Subject: Ask-A-Microscopist: etchant } } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I'm not sure there is a SAFE procedure for cleaning your window. Are you sure it is contaminated? (If the answer is yes, then the rest of this e-mail is irrelevant).
If you have high LN2 consumption on a detector that used to be good then almost certainly you have poor vacuum (I can't see why the crud inside the dewar would contribute to that - I have seen such deposits in the dewars of my detectors when they have been working fine - I've no idea what it is). With poor vacuum you would almost certainly have detector icing. (Have you run your detector conditioner?) Icing would lead to loss of sensitivity to low energy X-rays. A solution (that might only be temporary, if you have a significant leak) would be to warm the detector and pump it.
Having said all the above, we have 4 EDX detectors in use, the LN2 consumption varies widely, the most complex (a behemoth double gate-valve windowless on a VG STEM) has the lowest consumption of the 4, the simplest (on our JEOL 2010) has by far the highest consumption of any detector I've ever encountered, but it has always been that way (it was so bad we had it checked out by the manufacturer), and has always (and continues to) work just fine. Black magic, I suppose!
Tony.
At 09:55 AM 12/3/2002 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
** Anthony J. Garratt-Reed ** MIT Room # 13-1027 ** 77 Massachusetts Avenue ** Cambridge, MA 02139-4307 ** USA ** ** Phone: (+) 1-617-253-4622 ** Fax: (+) 1-617-258-6478 **
I clean my detectors (PGT) with Vertrel XF solvent recommended by PGT (see, for example, http://www.miller-stephenson.com/release_006.htm) Procedure: Remove collimator. Place detector so that window is vertical (if necessary, adjust its position later). From a pipette put a few drops of Vertrel on a top metal ring surrounding window. In no case should a single drop be placed directly on the window. See if liquid is running along window surface.
Good luck,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 3127 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} } Greetings Listers, } } Can anyone suggest a SAFE procedure for removing oil deposits from the } surface of the ATW2 (light element) window on my Oxford } detector? While } we're at it, does anyone know of a process for cleaning/polishing the } bottom of the inside of the dewar on the same detector. I } looked inside } and it appears to have a layer of deposits (dirt?) on the } bottom. Might } this be contributing to high LN2 consumption? TIA and MC and } HNY. Bill } Roberts } } } } This communication is for use by the intended recipient and contains } information that may be privileged, confidential or copyrighted under } applicable law. If you are not the intended recipient, you are hereby } formally notified that any use, copying or distribution of } this e-mail, } in whole or in part, is strictly prohibited. Please notify the sender } by return e-mail and delete this e-mail from your system. Unless } explicitly and conspicuously designated as "E-Contract Intended", } this e-mail does not constitute a contract offer, a contract } amendment, } or an acceptance of a contract offer. This e-mail does not constitute } a consent to the use of sender's contact information for } direct marketing } purposes or for transfers of data to third parties. } } Francais Deutsch Italiano Espanol Portugues Japanese } Chinese Korean } } http://www.DuPont.com/corp/email_disclaimer.html } } } }
I would appreciate comments from USERS about pro's and con's of different antivibration manufacturers systems.
----------------------------------------------- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2225 N Campus Drive Northwestern University Evanston, IL 60201, USA Tel: (847) 491-3996 Fax: (847) 491-7820 mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu -----------------------------------------------
I don't know about loading confocal image stacks, but Irfanview, a freeware product for PC (www.irfanview.com) allows you to rapidly flip through a large number of images, and recognizes all the usual formats.
If all the images are in a single directory you can view them sequentially by hitting space bar. It's the best I've found yet if you want to get a quick overview with minimum time and effort invested.
Wharton Sinkler UOP LLC Des Plaines, IL
-----Original Message----- } From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca] Sent: Tuesday, December 03, 2002 12:32 PM To: Microscopy-at-sparc5.microscopy.com
I very gently dribble Freon (yes, I still have a very little left) over the window, but I'm talking Be.
You should ask Oxford for their recommendations.
I have cleaned out my dewar by swirling methanol around in it, but of course you need to take it off your SEM for that. I understand that dirt in the dewar can increase noise, by providing nucleii for LN2 boiling, but my (limited) understanding is that high LN2 consumption is more likely to be caused by a deteriorated vacuum in the dewar, which really needs a pump/bake to fix it. I've just had that done to my PGT detector, improved the LN2 consumption remarkably and also resulted in the detector crystal running a few degrees colder, which should improve the noise performance but doesn't seem to have.
There's also the possibility of a slow leak in the window.
You should maybe ask Oxford also about recooling it, if you don't have a detector reconditioner built in to your interface there's a scary procedure involving boiling water which, I believe, drives adsorbed stuff off the crystal onto the getter.
cheers
rtch
Bill;
I have not tried this myself but did observe our service engineer clean the window on a 6 uM thick Be SUTW window. What he did was remove the dewar, detector housing and nosepiece from the electron column. With the housing held firmly, he ran droplets of acetone from a squeeze bottle down the face of the detector until it appeared clean. He DID NOT wipe the detector face, and that's important since even the slight pressure of a cotton swab may fracture it. Having told you all of this, I would still suggest asking the manufacturer [Oxford] whether they have a recommended cleaning method or would approve of what I just stated. Be certain that the column pressure is at atmosphere prior to attempting to remove the detector. Sudden changes in pressure can and do fracture these very thin EDX windows and that's an expensive mistake. I assume you have a diffusion pumped SEM because of the oil and not a turbomolecular pump OR extremely dirty specimens.
With regard to getting dirt out of the dewar, you may want to hold it upside down and blow it out with nitrogen at a low pressure. Of course it needs to be dry at the time. I doubt whether dirt would be affecting your LN2 consumption since it's principally evaporation that causes it do disappear and that's through the fill hole. Whomever is filling the dewar may not actually be topping it off and maybe it just appears to require filling more often. Of course when it's being filled and it's at room temp. the LN2 will boil and generate gas which will impede the filling process. This is remedied by simply filling the dewar partially and allowing it to cool and then topping it off. We have a 7 quart dewar and that generally is good for 7 days. If you are already doing all of this and still find yourself filling the dewar frequently, you may want to inspect it for a breach in the dewar wall itself. It should be evacuated between the inner and outer walls with no leaks.
I hope this is of some assistance.
Peter Tomic Anadigics, Inc.
-----Original Message----- } From: William H Roberts [mailto:William.H.Roberts-at-usa.dupont.com] Sent: Tuesday, December 03, 2002 9:55 AM To: Microscopy-at-sparc5.microscopy.com
Greetings Listers,
Can anyone suggest a SAFE procedure for removing oil deposits from the surface of the ATW2 (light element) window on my Oxford detector? While we're at it, does anyone know of a process for cleaning/polishing the bottom of the inside of the dewar on the same detector. I looked inside and it appears to have a layer of deposits (dirt?) on the bottom. Might this be contributing to high LN2 consumption? TIA and MC and HNY. Bill Roberts
This communication is for use by the intended recipient and contains information that may be privileged, confidential or copyrighted under applicable law. If you are not the intended recipient, you are hereby formally notified that any use, copying or distribution of this e-mail, in whole or in part, is strictly prohibited. Please notify the sender by return e-mail and delete this e-mail from your system. Unless explicitly and conspicuously designated as "E-Contract Intended", this e-mail does not constitute a contract offer, a contract amendment, or an acceptance of a contract offer. This e-mail does not constitute a consent to the use of sender's contact information for direct marketing purposes or for transfers of data to third parties.
Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean
I've had pretty good results with 10% ammonium persulfate (swabbing). This has the advantage that it isn't uncontrollably aggressive to base metals.
If health and safety considerations are met, dilute potassium cyanide / hydrogen peroxide (typically 5% of each mixed immediately before use and applied by swabbing ) performs well. This is particularly useful for gold over electroless nickel, as it simultaneously develops the structure of both metals and allows thickness measurements to be made that are not influenced by smearing of the gold.
John Twilley Conservation Scientist
by way of MicroscopyListserver wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (vladig-at-tht.net) from } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, } December 2, 2002 at 16:09:33 } --------------------------------------------------------------------------- } } Email: vladig-at-tht.net } Name: Vladimir Igoshev } } Organization: RIM } } Education: Graduate College } } Location: City, State, Country } } Question: Dear All, } } Does anybody know (can share) which etchant should be used for } Electroless Nickel plating? } } Thank you in advance for your input. } } Vladimir Igoshev, Ph.D. } Toronto, Canada } } ---------------------------------------------------------------------------
The traditional way of cleaning windows when I worked at Kevex 20 year ago was to run IPA over the window. The IPA was poured onto the metal cylindrical snout area above and away from the window and the IPA would flow over the window and into a beaker below. One did not direct IPA directly at the window. Most of our FS engineers would remove the detector from the chamber before doing this. I did it couple of times with it mounted. It was very tricky. Most FS engineers broke one or two windows in their careers so it wasn't that "Safe".
The reason for high LN consumption is a poor vacuum in the dewar. A dewar with a UTW needs to be baked and repumped every 5 years or so. The dirt at the bottom of the LN container does not make much difference. The poor vacuum also causes the snout to be cold which causes the window to collect oil. Send the dewar back to Oxford or a qualified service organization for dewar pumping and baking if you are not equipped to do this yourself.
Commercial Message: The Evactron anti-contaminator for removing oil and contamination from electron microscopes will clean your X-ray window safely and keep it from coming back. It combines RF plasma cleaning with Nitrogen purging to clean and remove oil form SEM chambers and EDS windows. More information is at www.SEMCLEAN.COM.
Notice: I invented the Evactron Anti-contaminator to solve oil problems and have an interest in selling more. Evactron is my registered trademark.
Ronald Vane
President
XEI Scientific
3124 Wessex Way
Redwood City, CA 94061
650-369-0133
650-363-1659
----- Original Message ----- } From: "William H Roberts" {William.H.Roberts-at-usa.dupont.com} To: {Microscopy-at-sparc5.microscopy.com} Sent: Tuesday, December 03, 2002 6:55 AM
Larry Hanke wrote :
{ {I have found that the things middle schoolers like to look { {at can be best observed with a good stereo microscope. A { {hand lens is great, but a stereo microscope with a light { {will be cooler to 5th grader.
And if you can find a TEM focusing binocular, it's a very good tool. Long working distance, x10 magnification, large objective diameter, that means much light. The child can use it outdoor with sun light, or home with a halogen desk light. And most of it are high quality optics. I have two from EM 300 TEM, one on a stand, the other for use in hand, and my children have enjoyed with it.
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Does some know a solution to etch a serie of PtFeCo alloys. They have 75% or 50% (weight) Pt and go from 0% Co - 100% Fe to the opposit. We want to mesure the grain size. I have some receipt for Pt or for FeCo alloys, but nothing for that situation, with a mixing of noble metal and FeCo.
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
We are trying to establish a reasonable service fee structure for our multi-user Core Facility, and would appreciate your input or information.
This is a basic fee structure for some of the common procedures performed at a biomedical shared core facility such as TEM (regular thin section as well as negative stain), SEM, immoEM, confocal, multiphoton microscopy, and broadband fluorescence microscopy. We generally do not cover material science projects.
The fee covers only the research-based activities at a private university with no involvement of clinical service. It should cover the personnel time and effort, materials required, as well as the facility use; but NO PROFIT is permitted.
I believe many of us are, or will be, involved in this issue. Any input, guidelines, suggestions, or special concerns are all welcome. Please feel free to contact me off-line if you prefer. Thank you for any help, and wish you a wonderful holiday season!
QC Yu
====================================== Qian-Chun Yu, MB, Ph.D. Director Biomedical Imaging Core Laboratory University of Pennsylvania School of Medicine 110 Richards Building Philadelphia, PA 19104
There are a number of outfits that sell software (and hardware) for applications such as you described. It's becoming a growing and competitive product in the microscopic imaging industry.
Vital Image Technology Scott Taylor Regional Manager 450 Portage Trail Cuyahoga Falls, Ohio 44221 ph. 330-940-3200 fax. 330-940-3222 st-at-vitalimage.com
Mager Scientific, Inc. Jim Uren PO Box 160 Dexter, Michigan 48310-0160 ph. 734-426-3885 fax. 734-426-3987 voice. 734-426-1116 juren-at-magersci.com
Either of them would be more than happy to send you literature describing the capabilities of their products.
Stu Smalinskas SKF USA Plymouth, Michigan
{Judy wrote: { {Fellow microscopists: { {I recently collected a lot of images at a single {sitting, each image seemingly better than the {previous one, definitely on a roll. However, the next {day I laboriously had to review a lot of images of {similar data and choose the best ones. { {What software are people using to browse through a {lot of images, as thumbnails, easily change their {names, perhaps add annotations, move selected ones {into new folders and create a catalogue. It should {also be able to recognize and load confocal image {stacks. { {Thank you {Judy { {Judy Trogadis {Bio-Imaging Coordinator {St. Michael's Hospital, 8Queen {30 Bond St. {Toronto, ON M5B 1W8 {Canada { {ph: 416-864-6060 x6337 {fax: 416-864-6043 {pager: 416-685-9219 {trogadisj-at-smh.toronto.on.ca
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Anyone out there have a viewing screen for a JEOL 100S they no longer need or are willing to part with? Quailty of the phosphor is not critical (we'll get it re-coated) but the qulaity of the aluminum backing IS important.
Figured its worth a shot.
Thanks
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 350 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
Does anyone know an environmental SEM facility located in the Baltimore-DC area? A friend of mine wanted to analyze samples without dehydration. I remember someone mentioned the environmental SEM, but did not take note.
An assossiate is looking for a facility with FESEM capabilities for nano materials research. Please contact Erin at emclaughlin-at-tritonsystems.com if you can be of assistance.
Best Regards, Peggy Miller UTHSCSA Department of Ophthalmology Lions Sight Research Foundation Ph: (210) 567-8460 Fax: (210) 567-8413
You may want to check our Metallographic Etch Database which you can find a link to on our website. Go to www.southbaytech.com and enter MED-1 in the keyword box in the upper right corner. If you open the PDF data sheet it will give you a link to a demo version of the database which will allow you to search by material for an etchant.
I hope this helps.
DISCLAIMER: South Bay Technology produces equipment and supplies as described above and, therefore, has a vested interest in promoting their use.
Best regards-
David
Faerber Jacques wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Does some know a solution to etch a serie of PtFeCo alloys. They } have 75% or 50% (weight) Pt and go from 0% Co - 100% Fe to the opposit. We } want to mesure the grain size. I have some receipt for Pt or for FeCo } alloys, but nothing for that situation, with a mixing of noble metal and } FeCo. } } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
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-----Original Message----- } From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca] Sent: Tuesday, December 03, 2002 10:32 AM To: Microscopy-at-sparc5.microscopy.com
Fellow microscopists :
I recently collected a lot of images at a single sitting, each image seemingly better than the previous one, definitely on a roll. However, the next day I laboriously had to review a lot of images of similar data and choose the best ones.
What software are people using to browse through a lot of images, as thumbnails, easily change their names, perhaps add annotations, move selected ones into new folders and create a catalogue. It should also be able to recognize and load confocal image stacks.
Thank you Judy
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 8Queen 30 Bond St. Toronto, ON M5B 1W8 Canada
} } What software are people using to browse through a lot of images, as } thumbnails, easily change their names, perhaps add annotations, move } selected ones into new folders and create a catalogue. It should also } be able to recognize and load confocal image stacks. }
IrfanView (free from Irfanview.com) has the great property that if you have one image open, there are two toolbuttons which enable you, with one mouseclick, to open the next or the previous image in the directory.
Thumbsplus, which I got free with a magazine, has a good thumbnail image viewing and title-filing system.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
A good idea is YS100 from Nikon, it cost around $ 1,400.00 website www.nikonusa.com
Michael Cammer {cammer-at-aecom.yu.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I received the following email from a friend in DC and don't know how to } help her because I hopelessly burdened with expertise only in high end systems. } My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, but } any suggestions are appreciated. } ----------------- } HELP!!!! All Katie wants for Christmas is a good microscope. She wants to } be able to really see things and does not want a "kid" microscope. I need } something with good optics but not expensive. Used is fine. Any ideas, } brand names, places to look? Thanks. } ------------------ } } } } ____________________________________________________________________________ } Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. } Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 } (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/ } } }
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QC We went through revising rates for a multi-user and service facility just last year. First thing you have to know is actual cost based on time, materials, salaries, hard costs (such as service contracts), etc. Based on the number of procedures and hours each piece of equipment has been used, you can figure past costs and make projections as to future costs. Then you need to know how much support you will get to subsidize costs and procedures. Once all this data is known, you can determine final costs for each procedure or equipment by balancing anticipated actual income vs. funds for subsidizing.
For example: An SEM is used 400 hrs/year with a $14,000 service contract + $1000 supplies (nitrogen, etc). Actual cost per hour is $37.50. But perhaps you have a TEM that is only used 200 hr/year with the same cost so actual cost is $75/hr. You may be able to subsidize (at least initially if you are building a user base) the TEM rate to make it also $37.50/hr which may be all your researchers can afford to pay. If you have to figure salaries in than the cost will obviously go much higher.
In my facility we do not have to raise funds to cover salaries. We also have an ample budget to cover service contracts and other costs at the present rate. However we must bring in sufficient revenue to cover all increased costs plus materials and new equipment not obtained on grants. Therefore we can subsidize rates heavily for internal users (external pay full cost) and so can keep rates for use and for service quite low. An example: $11/hr for SEM and TEM use by trained users, $26/hr if technical help is needed.
Costs for specimen preparation is based on actual anticipated costs of consumables plus $15/hr technical charge. Consumables, such as film, are priced at replacement cost as we also cannot make a profit. Multi-users provide most of their own consumables (fixatives, embedding resins, photographic paper, etc) so we don't have to keep track of all that stuff...that would be a nightmare.
Hope this helps. Feel free to contact me if you need more information. Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building West Lafayette, IN 47907
On 12/4/02 5:04 AM, "Qian-chun Yu, Ph.D." {qcyu-at-mail.med.upenn.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Colleagues, } } We are trying to establish a reasonable service fee structure for our } multi-user Core Facility, and would appreciate your input or information. } } This is a basic fee structure for some of the common procedures performed } at a biomedical shared core facility such as TEM (regular thin section as } well as negative stain), SEM, immoEM, confocal, multiphoton microscopy, and } broadband fluorescence microscopy. We generally do not cover material } science projects. } } The fee covers only the research-based activities at a private university } with no involvement of clinical service. It should cover the personnel time } and effort, materials required, as well as the facility use; but NO PROFIT } is permitted. } } I believe many of us are, or will be, involved in this issue. Any input, } guidelines, suggestions, or special concerns are all welcome. Please feel } free to contact me off-line if you prefer. Thank you for any help, and } wish you a wonderful holiday season! } } QC Yu } } ====================================== } Qian-Chun Yu, MB, Ph.D. } Director } Biomedical Imaging Core Laboratory } University of Pennsylvania } School of Medicine } 110 Richards Building } Philadelphia, PA 19104 } } Phone: 215-573-7766 (Voicemail) } FAX: (215)573-2259 } E-mail: qcyu-at-mail.med.upenn.edu } } } }
Gordon; I would think that a very bright 4-5th grader today would have seen everything that could be seen with a magnifier already. Splurge and get that kid a real microscope! I recall getting a microscope (cheap, but a real one) for my 8th birthday 45 years ago, and look what happened-I'm spending the rest of my life looking through microscopes! MSA is graying, and is in need of fresh blood, so we should all do our part to inspire the next generation.
John Mardinly Desk: 408-765-2346 Pager: 877-277-1182
-----Original Message----- } From: Gordon Nord [mailto:gnord-at-mindspring.com] Sent: Monday, December 02, 2002 8:26 PM To: Michael Cammer Cc: Microscopy List
Michael,
I had this dilemma a few years ago. The solution is not a microscope (expensive and not portable) but a good wide field hand lense. She can examine things and carry the hand lense around. It is perfect for the beginner.
You can visit {http://www.edmundoptics.com} and look at the magnifiers. For $50 she can get a 12x magnifier.
On Monday, December 2, 2002, at 02:03 PM, Michael Cammer wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I received the following email from a friend in DC and don't know how } to help her because I hopelessly burdened with expertise only in high } end systems. } My thoughts have been Edmunds Scientific, the Fisher catalog or eBay, } but any suggestions are appreciated. } ----------------- } HELP!!!! All Katie wants for Christmas is a good microscope. She wants } to } be able to really see things and does not want a "kid" microscope. I } need } something with good optics but not expensive. Used is fine. Any ideas, } brand names, places to look? Thanks. } ------------------ } } } } ____________________________________________________________________________ } Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of } Med. } Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY } 10461 } (718) 430-2890 Fax: 430-8996 URL: } http://www.aecom.yu.edu/aif/ } } } Dr. Gordon Nord Senior Scientist Environmental Sciences Laboratory Brooklyn College Brooklyn NY 11210
Judy, You may also want to check out XnView. It's open source software so it's free. It does everything ACDSee does, including printing out contact sheets. I also use ACDSee because its tightly integrated to Windows. The only problem with XNView is that the installation requires a bunch of libraries be installed first. As for viewing confocal stacks, you might want to check out ImageJ which is a extremely nice rewrite of NIH Image using java.
Glenn Poirier tel: (613) 947-9833 Microbeam Specialist FAX: (613) 996-9673 Mining and Mineral Sciences Laboratory 555 Booth St CANMET Ottawa, ON K1A OG1
-----Original Message----- } From: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: 04 December, 2002 12:01 PM To: Judy Trogadis; Microscopy-at-sparc5.microscopy.com
ACDC!
John Mardinly Desk: 408-765-2346 Pager: 877-277-1182
-----Original Message----- } From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca] Sent: Tuesday, December 03, 2002 10:32 AM To: Microscopy-at-sparc5.microscopy.com
Fellow microscopists :
I recently collected a lot of images at a single sitting, each image seemingly better than the previous one, definitely on a roll. However, the next day I laboriously had to review a lot of images of similar data and choose the best ones.
What software are people using to browse through a lot of images, as thumbnails, easily change their names, perhaps add annotations, move selected ones into new folders and create a catalogue. It should also be able to recognize and load confocal image stacks.
Thank you Judy
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 8Queen 30 Bond St. Toronto, ON M5B 1W8 Canada
Does anyone knows of a free or accessible software to compose in-focus 2-D images from several images in different focal planes? Martin
Martin J. Ramirez Division of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York 10024, N.Y. USA Tel: (212) 769 5609 Fax: (212) 769 5277 email: ramirez-at-amnh.org
Peter et al, I've been given 2 very good reasons not to use acetone (Freon TF or isopropyl alcohol are preferred).
1) A long time ago I was told that the 7.5u Be window was actually porous and the micro-pores were actually filled with oil - which acetone very effectively removes, leaving a deteriorating vacuum.
2) More recently on this listserver it was noted that acetone is very likely to attack whatever adhesive/bonding agent is used to fix the window in place. Also a vacuum problem.
I would definitely ask Oxford and if it's a light element window... I had one break while gently removing the collimator. Neither I nor my customer were very pleased about that! I was going to clean it for him with IPA but there was this terrible sucking sound...
High lN2 consumption is due to poor dewar vacuum. If the dewar is tight, it'll be good for 20 years or more. If the consumption has risen in a newer dewar, there is a leak that needs to be fixed first.
Good luck, you may need it.
Ken Converse owner Quality Images third party SEM service Delta, PA 17314
Peter Tomic wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I would like to do some X-ray analysis on the JEOL 733 with an electron probe size of 10nm-100nm.
Does anyone know whether this is feasible and what probe current I can expect? What is the smallest probe size and corresponding current that can be achieved on the 733? Also, I'd be interested to know the Cs, Cc and typical beam convergence semi-angle for the standard objective lens at 15mm working distance.
Ken Converse wrote: ============================================================= Peter et al, I've been given 2 very good reasons not to use acetone (Freon TF or isopropyl alcohol are preferred).
1) A long time ago I was told that the 7.5u Be window was actually porous and the micro-pores were actually filled with oil - which acetone very effectively removes, leaving a deteriorating vacuum.
2) More recently on this listserver it was noted that acetone is very likely to attack whatever adhesive/bonding agent is used to fix the window in place. Also a vacuum problem.
I would definitely ask Oxford and if it's a light element window... I had one break while gently removing the collimator. Neither I nor my customer were very pleased about that! I was going to clean it for him with IPA but there was this terrible sucking sound... ============================================================ If beryllium foil was porous to the extent indicated by Ken, it would be virtually impossible to make it vacuum tight (and these foils are made by intention to be vacuum tight).
Secondly, beryllium windows and foil should be UHV clean when they leave the manufacturer of the foil. Having the surface impregnated with oil as described just does not sound right (or possible).
Attempting to clean an ultra-thin beryllium window unsupported would be an extremely delicate operation. If not done properly, the window could be broken, especially if it were under differential pressure at the time. The best way would be to equalize the pressure, preferably at atmosphere if possible, and then carefully provide a solid support for the window during cleaning. This would reduce risk of damage, but not eliminate it. Once the window is mounted, it is always going to be difficult to clean, especially, to clean safely. Of course, this is not the kind of thing that could be done in the typical SEM/EDS laboratory.
Chuck
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Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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Under the "faint hope", "last chance" and "final straw" category, would anybody out there have an old Varian VacIon Pump Control Unit Model 921-0062 (circa 1989) out there? Ours (which controls the ion pump on our ESEM) is in dire need of a new reset relay. This is a complex little device, a Potter-Brumfield 1537, which is no longer made. P-B says it's obsolete, and was only made in small numbers under special order (to Varian, I assume). Varian, helpfully, says "Gee, that part's obsolete, we don't have any, try Potter-Brumfield". If I can obtain one from a retired Varian unit, that would be a nice easy fix. Failing that, our in-house electronics technologists/engineers will have to bypass it some way, and replace it with an off-the shelf unit, though this would need some modifications. And the instrument's down until that happens. I've had luck in the past obtaining equally arcane items from the folks on this List, so I'm giving it a shot.
Frank Thomas Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia
Can anyone direct me to an online source where i can try to identify molds and fungi? We had some green stuff growing on a large wood spool. Under the SEM the stuff looks like bunches of grapes with each "grape" about 2 microns. Any of you biological types out there want to hesitate a guess? Mold spores? fungus? Any help would be appreciated.
Cheers
Paul D. Nolan Electron Optics
Alcan International Limited Kingston Research and Development Centre P.O.Box 8400, 945 Princess Street Kingston, Ontario K7L 5L9
I know there has been some discussion about microscopes for kids lately but I'd like to ask about a specific scope. A co-worker has a 6 year old who has asked for a microscope. He is looking at the "Quantum Big Screen Microscope" through the Learning Resources catalog but is concerned about the quality of the microscope. Does anyone know anything about the quality of this instrument or of the manufacturer? I'm assuming everything is plastic - does that mean it will scratch easily or are the "optics" protected? How good are the images it produces? Any comments on this particular microscope or suggestions for a better one would be greatly appreciated.
The University of Minnesota Characterization Facility has a new position opening up for an EM person with some experience in TEM sample prep. See the link below for more details on how to apply, etc. All applications must go through our HR department. You may e-mail me, but unless you formally apply, you cannot be considered.
http://www1.umn.edu/ohr/employ.html
The link for the position description (partially copied below) is: http://www1.umn.edu/ohr/jobs/R117717.html
Requisition#: 117717 Job Title: Junior Scientist Working Title: Electron Microscopy Technologist Job Code Pay Range: $ 12.36 -$ 19.99 /Hour Qualifications: A two-year associate degree in electron microscopy and 2 years experience in electron microscopy with experience in standard TEM techniques including sample preparation, embedding, thin sectioning and positive and negative staining. SELECTION CRITERIA/PREFERRED: Ranking primarily based on total years of experience in electron microscopy. Preference will be given to persons having an associate degree and experience in biological or medical science related area transmission electron microscopy. Bachelor's degree, experience in sample preparation techniques, TEM and digital imaging will be taken into consideration in ranking. Duties: The Institute of Technology's Characterization Facility is seeking candidates for a position as an electron microscopy technologist in an active core research laboratory. The facility houses 4 TEMs and 6 SEMs, as well as appropriate preparation equipment. In addition there is an integrated suite of specimen characterization equipment maintained within the facility. The position requires an individual experienced in standard TEM techniques including samples preparation, embedding, thin-sectioning, positive and negative staining. Experience in cryo-TEM and facility in digital imaging would be a benefit. The applicant will be expected to perform routine use and maintenance of the microscopes and preparation equipment, as well as basic training of new users of the facility. 40% EM operation and maintenance. 40% Sample Preparation. 10% Training. 10% Lab Administrative duties.
******************************************************************** Michael L. Boucher Sr. mboucher-at-tc.umn.edu Lab Manager Rm 55 Office Ph 612-624-6590 I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594 University of MN Fax 612-625-5368 12 Shepherd Labs 100 Union Street S.E. Minneapolis, MN 55455 http://resolution.umn.edu ********************************************************************
What does that relay do? What's on the input, what's on the output? Is it just a plain relay, or a latching relay, or a time delay relay? Or anything else?
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax (678)467-0012 mobile
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----- Original Message ----- } From: Frank Thomas {thomasf-at-gsca.NRCan.gc.ca} To: {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, December 05, 2002 8:48 AM
} I apologize! I accidently sent the first draft which had the wrong dates. } The correct announcement is below. } } New York Microscopical Society } } 30 North Mountain Avenue } } Montclair, NJ 07042 } } } } } } } } } Bernard Friedman } } } Memorial Workshop } } } } } } } Digital Image Capture and Management in Microscopy } } May 8, 2003 } } } A course on Digital imaging in light microscopy which will cover the } following topics: } } } Optical Limitations in Light Microscopy...Photographic Imaging Strategies... } Digital Imaging Strategies...Selection of Digital Capture (Camera vs. } Scanner)... } } Image Processing of Captured Images...Image File Formats...Printing } Images... Color Management Systems...Database Management } Software...Presentation Software for Oral Reports...Website } Performance...Integration of Image Data with Sample Information, } Calibration, Other Data & Reports...Acrobat and html Software for Written } Reports and Archives...Examples of Efficient, Low Cost Image Handling } Systems... } } Examples of Electronic Microscopy Reports and Databases } } } The course instructors are Mary and John McCann of McCann Imaging. } } WHEN: Thursday, May 8, 2003, from 9 A.M. to 5 P.M. } } WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043 } } COST: $350 for N.Y.M.S. members, $380 for non-members (includes membership) } Lunch and course materials are included. Checks made out to N.Y.M.S. } } HOW: Register using the form below. Limited to the first 12 registrants. } } Return form with a check to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ } 07410. } } FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 e-mail } donoleary-at-att.net } } PLEASE POST } } -------------------------------------------------------------------------- -- } ------------------------------------------------ } } Digital Image Capture Registration Form } } N.Y.M.S. Member_________________ ($350) Non-Member__________($380) } } Name____________________________________________________________________ } } Address__________________________________________________________________ } } Phone (W)_____________________(H)_____________________Fax_________________ } } e-mail________________________________________ } }
I'm looking for some feedback/opinions/help on a recurring problem we're having with short filament life in our TEM. The last one got 64 hours....way, way less than experience says we should be getting.
Filament tip to Wehnelt distance is per manufacturer's specs (1 mm), set according to manufacturer's procedures. The gun is meticulously cleaned upon replacement, with all visible tungsten being removed with a combination of sonication in ammonia and polishing with metal polish. The gun components are then cleaned in ultrapure water, ethanol, and acetone, carefully dusted with compressed air, and reinstalled while wearing clean gloves. The anode chamber is dusted and O-ring inspected and cleaned, if necessary. Gun vacuum is good. We run the filament at 10 uA and it's saturating where the service engineers say it should be saturating at, in terms of bias settings and filled-in halos, etc.
The burnt out filaments exhibit normal narrowing , but the wire below the failure shows a small bulb, indicative of oversaturation. The puzzling part to me is that we're getting sheets (literally) of tungsten around the Wehnelt aperture, so thick that they are peeling away. Picture a dry mud-flat with patches of mud curling back on themselves away from the ground, only made of tungsten and surrounding an aperture! I have been told that this tungsten can be deposited in an instant as the filament dies a violent death, but I have never seen it on any other instrument, ever. This is happening whether I install the new filament or the service engineers install them. Oh, yeah, the filaments are OEM standard issue, not rebuilds, but in the past it has happened to rebuilds, too.
Any ideas? Thanks!
Sick of installing filaments, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
} Attempting to clean an ultra-thin beryllium window } unsupported would be an } extremely delicate operation. If not done properly, the } window could be } broken, especially if it were under differential pressure at } the time. The } best way would be to equalize the pressure, preferably at } atmosphere if } possible, and then carefully provide a solid support for the } window during } cleaning. This would reduce risk of damage, but not } eliminate it. Once the } window is mounted, it is always going to be difficult to } clean, especially, } to clean safely. Of course, this is not the kind of thing } that could be } done in the typical SEM/EDS laboratory. } } Chuck } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President 1-800-2424-SPI } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.2spi.com } ######################## } ============================================ } } } }
Hi dear Lisetservers; I have to buy a new printer for my EM lab (our old printer refuses to work properly). Searching the web site, I came across a printer Minolta-Magicolor 2200 DeskLaser with 1200 dpi and up to 96 Mb RAM. I would like to use it for office printing as well as for working quality electronmicrographs (I expect a lot form working quality). Has any of you used this kind of printer? I would like to know how reliable it is and what is the quality of printing. Can it be used for printing electronmicrographs? Thanks Dorota
Hello; Our EM lab received a grant to buy a new knifemaker (for histo and thin knives). I have a few questions to you: 1.Is Leica the only supplier of knifemakers in North America? 2.Are there any other supplier? 3.If yes, what is the quality of their equipment and how can I reach them? Please send your replies. Thanks Dorota
Hi Paul, Can you send me, or the microscopy listserve, the SEM picture of your mold? Illustrated Genera of Imperfect Fungi by Barnet & Hunter (APS Press, 1998) might help. Dean Abel Biological Sciences 138BB University of Iowa Iowa City IA 52242-1324
At 10:31 AM 12/5/2002 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
The spacial resolution with which you can do EPMA analysis is ultimately limited not by the probe diameter but by the finite interaction volume which is a result of the incident electrons' not giving up all of their energy at the point of impact, but by multiple interactions within a teardrop-shaped volume below the surface. The volume's diameter at the surface depends on the accelerating voltage and on the specimen's mean atomic number, but is typically a couple of microns.
A picture is better, there are several good articles on EPMA on the www.
One, by James Wittke of Northern Arizona University, is at http:jan.ucc.nau.edu/~wittke/Microprobe/ProbeNotes, another is the set of class notes for course 12.141 by Nilanjan Chatterjee, on the MIT website.
So your hoped-for resolution of 10nm to 100nm is not attainable, unless you go to a thin-film sample.
cheers
rtch
} From: "James, Ed" {Ed.James-at-kla-tencor.com} To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
} I } had one break while gently removing the collimator. Neither I nor my } customer were very pleased about that! I was going to clean it for } him with IPA but there was this terrible sucking sound... }
Yes, it's a dreadful sound, much more expensive than the clicking of a dying ZIP disk.
I, too, have heard it.
In my case it was an expansive hand-waving gesture by the detector's owner, whose finger-nail just clipped the window.
The detector was dead anyway, so we could almost laugh.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Dorta, The Magicolor 2200 is a pretty good printer - fast, reliable, never a paper jam. It gives good color rendition/reproduction, has a built in fast Ethernet port. However(there is always one), the actual resolution is 600dpi hardware, 1200dpi interpolated. But, by using a good quality coated inkjet or laser paper, digital images come out very well indeed. Find a paper with a brightness of at least 95, it really makes a difference. I sometimes bundle this printer with the ORION SEM Digital Imaging System my company sells. My customers really like it, just for the reasons you inquired about. DISCLAIMER - I do not work for the Minolta Corporation, just a satisfied user/reseller of their products.
Gary M. Easton, Pres. Scanners Corporation 410-857-7633
----- Original Message ----- } From: "Dorota Wadowska" {wadowska-at-upei.ca} To: {microscopy-at-sparc5.microscopy.com} Sent: Thursday, December 05, 2002 12:13 PM
Randy, It sounds like you may have some instability in your filament drive circuit. At some point the drive increases and you don't notice it because you're already saturated. I just had a similar problem with a customer's SEM. As near as I can tell it was just some oxidation on the low voltage connector for the filament drive on the HV tank. After exercising the connector, it's been OK for over a week. I've also seen flaky Op Amps and transistors intermittently do this sort of thing, also. It's going to require some long-term monitoring of the filament drive circuit. If you're lucky, it'll be on the low voltage side.
Ken Converse owner Quality Images third party SEM service Delta, PA
Tindall, Randy D. wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hello All, we are having yet another clear out of old cupboards with unused equipment. If anyone is interested in having two Nuclear Enterprises scintillation counters (Be window) model DM1-1 before they go for landfill (with Be removed, of course), let me know asap. You will have to pay for collection.
======================================================================= This e-mail is intended for the person it is addressed to only. The information contained in it may be confidential and/or protected by law. If you are not the intended recipient of this message, you must not make any use of this information, or copy or show it to any person. Please contact us immediately to tell us that you have received this e-mail, and return the original to us. Any use, forwarding, printing or copying of this message is strictly prohibited. No part of this message can be considered a request for goods or services. ======================================================================= Any questions about Bookham's E-Mail service should be directed to postmaster-at-bookham.com.
They sell the GKM knifemaker, which breaks rectangles instead of strips, and can make knives up to about 1/2" wide. I found this knifemaker to be much better than any other. Personal experience only, mind, and I have no ties to the company.
Phil
} Hello; } Our EM lab received a grant to buy a new knifemaker (for histo and } thin knives). I have a few questions to you: } 1.Is Leica the only supplier of knifemakers in North America? } 2.Are there any other supplier? } 3.If yes, what is the quality of their equipment and how can I reach } them? } Please send your replies. } Thanks } Dorota
-- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax)
You are correct on the acetone. It was IPA. My apologies. It's my fading memory that needs to be addressed.
I should add that the cleaning was done while the detector was still in the collumnator with the nose piece still in place. The reason this was done in the first place was that the detector was on an old Hitachi S570 with diff. pump and someone tried to pump the column down while the exchange chamber door was wide open! The entire column had to be cleaned including detector.
Multi-user facilities are a nightmare! I often suggest coin operated analytical equipment to pay for the service contracts.
Peter
-----Original Message----- } From: qualityimages [mailto:qualityimages-at-netrax.net] Sent: Wednesday, December 04, 2002 8:00 PM To: Peter Tomic; Microscopy
Wow! Thanks for all the replies that I've received on this topic. They're still coming in! Lots of very interesting possibilities, ranging from chronic oversaturation, to potential problems with ammonia cleaning, to power supply glitches.
If nobody objects, I would like to compile a summary of these responses for the list.
Also, I was asked why I didn't name the equipment that the problem was on. It's because on several occasions when I have done so, manufacturers and/or their service people have gotten quite excited about implied public criticism of their equipment, when I was just looking for general information and ideas. Except for the filament life problem, we are very happy with this scope, so I didn't see a real need to be more specific in this case. If anyone is curious, I'll be happy to identify the scope off-list.
Thanks again. You folks are great!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
I also have several spare IoP series conference proceedings which are available if anybody wants them.
Ser. No. 67 Microscopy of Semiconducting Materials 1983 Ser. No. 76 Microscopy of Semiconducting Materials 1985 Ser. No. 67 Microscopy of Semiconducting Materials 1983 Ser. No. 100 Microscopy of Semiconducting Materials 1989 Ser. No. 117 Microscopy of Semiconducting Materials 1991 Ser. No. 134 Microscopy of Semiconducting Materials 1993 Ser. No. 146 Microscopy of Semiconducting Materials 1995
Ser. No. 68 Electron Microscopy and Analysis 1983 Ser. No. 90 Electron Microscopy and Analysis 1987 Ser. No. 93 Volumes 1 and 2 EUREM 1998
As before, first come, first served, & your collection method has to be as cheap as me putting them in the bin!
Richard _______________________________ Richard Beanland Analytical Services Bookham Technology plc Caswell, Towcester, Northamptonshire NN12 8EQ UK Tel: +44 (0) 1327 356362 Fax: +44 (0) 1327 356398 http://www.bookham.com
======================================================================= This e-mail is intended for the person it is addressed to only. The information contained in it may be confidential and/or protected by law. If you are not the intended recipient of this message, you must not make any use of this information, or copy or show it to any person. Please contact us immediately to tell us that you have received this e-mail, and return the original to us. Any use, forwarding, printing or copying of this message is strictly prohibited. No part of this message can be considered a request for goods or services. ======================================================================= Any questions about Bookham's E-Mail service should be directed to postmaster-at-bookham.com.
Sorry, don't have one - but I get parts for our Varian ion pump from an outfit called Duniway stockroom. At http://www.duniway.com/html/ion_contro-surplus.htm they list a rebuilt 921-062 control unit for $1250. Maybe they can rebuild yours or do some kind of swap. Hope this helps. Their number is 1-800-446-8811.
Regards, Andrew
Disclaimer: I don't work for Duniway of have any financial interest in the company; just a satisfied customer.
At 09:48 AM 12/5/2002 -0400, Frank Thomas wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
On Thursday, December 5, 2002, at 09:27 AM, Tindall, Randy D. wrote:
} I'm looking for some feedback/opinions/help on a recurring problem } we're having with short filament life in our TEM. The last one got 64 } hours....way, way less than experience says we should be getting. } } We run the filament at 10 uA and it's saturating where the service } engineers say it should be saturating at, in terms of bias settings } and filled-in halos, etc. } } The burnt out filaments exhibit normal narrowing , but the wire below } the failure shows a small bulb, indicative of oversaturation.
} Any ideas? Thanks! } Dear Randy, I have two ideas: 1) Filament lifetime is longer if the current is just at the saturation point; is 10 uA higher than this? If there is evidence of oversaturation, the current may be too high. 2) Did you heat the filaments in, say, a vacuum evaporator before you installed them? Sometimes the first heating will cause the filament to warp due to stresses built up during manufacture; this will cause the position of the filament to change if the warping occurs in the Wehnelt and may contribute to shorter life and lower beam current. Are all the filaments from the same batch? Good luck. Yours, Bill Tivol EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
I got my neurologist a used single power stereo microscope that his 5 year old loves. Some think that monocular work better for young kids because there is less to adjust. When you find them monocular low powered scope that have inverting prisms sell for a song.
http://www.couger.com/microscope/links/gcnewbuy.html Is a page that I keep that cover a lot of issues on buying used microscopes form several peoples points of veiw. I am convinced you get a great deal more for your money buying a good used brand name scope if you can find one that fits you needs and doesn't have too many gadgets for the kids. I have sitting on my bench right now a good recent imported Asian Scope, a classic B&L Stereo Zoom 7 and a old AO Cycloptic. They are all satisfactory scopes but I reach for the old AO because of the brighter image than the rest. I am cleaning the Asian Scope and while difficult to align it is not much more so than any other. The prisms aren't coated with aniti reflective coatings. The images aren't as bright because the telescopes are smaller and there is plastic used in many places.
I have no commercial interests is selling microscopes but I will always do everything I can to help someone get a kid started in science so anything I can do to help please fell free to ask.
Gordon Gordon Couger gcouger-at-couger.com
I collect links on information related to light microscopes. http://www.couger.com/microscope/links/gclinks.html Please forward any links or information you think might be useful to others.
----- Original Message ----- } From: "Anjeanette Ormonde" {Anjeanette.Ormonde-at-unilever.com} To: {Microscopy-at-sparc5.microscopy.com} Sent: Thursday, December 05, 2002 8:46 AM
Thumbs Plus from Cerious Software.
Similar to ACDC, but I happen to like it better. We use it for
- Image file management - Contact Sheet Printing - Batch image processing - Web pages with thumbnails
We use it on several different computers.
http://www.cerious.com/
---------------------------------------------------------------------------- -------------------------------- John Reffner, ACTC Microscopy Group Leader, Rohm and Haas Co. 215-619-5283 JReffner-at-rohmhaas.com ------------------- Opinions expressed are mine and ----------------------- ------------------- not those of Rohm and Haas Company -----------------------
2) Energy Beam Sciences manufactures both Triangular and Ralph (Long) Glass Knifemakers. RMC Boeckeler also makes a Triangular Knifemaker. Those are the only others I am aware of.
3) EBS has been manufacturing these Knifemakers for many years and has many satisfied Customers around the world. You can reach us at ebs-at-ebsciences.com or by phone on (413) 786-9322.
Sincerely, Michael R. Nesta General Manager Energy Beam Sciences, Inc. 11 Bowles Road Agawam, MA 01001-2925 Tel: (413) 786-9322 Fax: (413) 798-2786 "Adding Brilliance to Your Vision"
Disclaimer: Writer is an employee of and has a financial interest in Energy Beam Sciences, Inc.
-----Original Message----- } From: Dorota Wadowska [mailto:wadowska-at-upei.ca] Sent: Thursday, December 05, 2002 12:20 PM To: microscopy-at-sparc5.microscopy.com
Hello; Our EM lab received a grant to buy a new knifemaker (for histo and thin knives). I have a few questions to you: 1.Is Leica the only supplier of knifemakers in North America? 2.Are there any other supplier? 3.If yes, what is the quality of their equipment and how can I reach them? Please send your replies. Thanks Dorota
Afternoon Darota: I bought a JB-4 Knifemaker years ago when the JB-4 was first produced by Dupont. That entire system is now manufactured and sold by Energy Beam Sciences.
They have both a triangular knife maker and a Ralph knife maker. I only have experience with the original triangular knife maker and was happy with it.
I have no relationship to EBS other than as a customer of Dupont years ago.
Regards,
Fred Monson
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone: 610-738-0437 eMail: fmonson-at-wcupa.edu
-----Original Message----- } From: Dorota Wadowska [mailto:wadowska-at-upei.ca] Sent: Thursday, December 05, 2002 12:20 PM To: microscopy-at-sparc5.microscopy.com
Hello; Our EM lab received a grant to buy a new knifemaker (for histo and thin knives). I have a few questions to you: 1.Is Leica the only supplier of knifemakers in North America? 2.Are there any other supplier? 3.If yes, what is the quality of their equipment and how can I reach them? Please send your replies. Thanks Dorota
Good for you, Randy! I cringe every time I see someone posting a doubtful criticism of a named manufacturer on the public listserver when, oftentimes, the problem is due to the poster's poor technique or failure to understand the basics of microscopy.
Who can forget the criticism of a TEM manufacturer's so-called unstable specimen stage when it turned out that the complainer did all of his examination, focusing, and plate exposure of biological thin sections at crossover with the brightest C1 setting because "It's easier to work with a bright image."
Ron Anderson
-----Original Message----- } From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu] Sent: Friday, December 06, 2002 9:46 AM To: microscopy-at-sparc5.microscopy.com
Wow! Thanks for all the replies that I've received on this topic. They're still coming in! Lots of very interesting possibilities, ranging from chronic oversaturation, to potential problems with ammonia cleaning, to power supply glitches.
If nobody objects, I would like to compile a summary of these responses for the list.
Also, I was asked why I didn't name the equipment that the problem was on. It's because on several occasions when I have done so, manufacturers and/or their service people have gotten quite excited about implied public criticism of their equipment, when I was just looking for general information and ideas. Except for the filament life problem, we are very happy with this scope, so I didn't see a real need to be more specific in this case. If anyone is curious, I'll be happy to identify the scope off-list.
Thanks again. You folks are great!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
Good for you, Randy! I cringe every time I see someone posting a doubtful criticism of a named manufacturer on the public listserver when, oftentimes, the problem is due to the poster's poor technique or failure to understand the basics of microscopy.
Who can forget the criticism of a TEM manufacturer's so-called unstable specimen stage when it turned out that the complainer did all of his examination, focusing, and plate exposure of biological thin sections at crossover with the brightest C1 setting because "It's easier to work with a bright image."
Ron Anderson
-----Original Message----- } From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu] Sent: Friday, December 06, 2002 9:46 AM To: microscopy-at-sparc5.microscopy.com
Wow! Thanks for all the replies that I've received on this topic. They're still coming in! Lots of very interesting possibilities, ranging from chronic oversaturation, to potential problems with ammonia cleaning, to power supply glitches.
If nobody objects, I would like to compile a summary of these responses for the list.
Also, I was asked why I didn't name the equipment that the problem was on. It's because on several occasions when I have done so, manufacturers and/or their service people have gotten quite excited about implied public criticism of their equipment, when I was just looking for general information and ideas. Except for the filament life problem, we are very happy with this scope, so I didn't see a real need to be more specific in this case. If anyone is curious, I'll be happy to identify the scope off-list.
Thanks again. You folks are great!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
Dear Randy, It sounds like you are doing all the right things, except checking the saturation level after the filament has run for a while. I re-check the saturation level every running hour for at least five hours and then once a day after that, because the saturation point actually decreases slightly as the filament ages. This is a curve, with the steepest drop in saturation temp at the beginning. The other thing I do is run with the slightest dark spot remaining in the condenser crossover image. This is a quick way to check that you are still slightly undersaturated. Good luck. Mary ----- Original Message ----- } From: "Tindall, Randy D." {TindallR-at-missouri.edu} To: {microscopy-at-sparc5.microscopy.com} Sent: Thursday, December 05, 2002 9:27 AM
Phil, I do agree that GKM knifemaker is good. Unfortunately I have extremely bad experience with EM Sciences. When I am shopping for equipment, my rule is that I have to see how equipment works before I buy it (demo or exibition). Like car, you have to try it first. I was asking EM Sciences for the demo. They refused. Finally after discussion on the high level they did agree to sent to me instrument for demo and I'll buy it if I like it... I DID sign a special 5 pages Contract (never did before), they supposed to sent instrument after that. Now it's about a year as they DO promise to send the instrument. When I called them a month later after signing the Contract - it was a panic on the other end - they were urgently assembling the instrument (they did not have any at hand). So, they were lay when sign the Contract (there were exact dates on the Contract for shipping). I told them, OK, I'll wait. So, I am waiting and waiting... until now. I do have all paperwork from that case to verify that the story is true. It looks to me that Leica is only a possibility for majority of us. I really don't like old RMC knifemaker, but newer try the current model. Personally I did not try 10 mm on Leica, but 8 mm knifes were really good, no complains. I don't have Leica instrument, I sort of wait for response from EB Sciences... meantime using 10 mm W-carbide knifes from DDK. By the way, EB Sciences was trying to sell to me Leica instead GKM, so they definitely have some problems with GKM. I think this is a purpose of our ListServer: to share experience, bad and good. I wish everyone a great Holiday Season and according Russian tradition, I wish, all your wishes (at least a few of them) become true in the coming New Year. Sergey
At 05:59 AM 12/6/2002, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amostaghimi-at-onyx-pharm.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, December 6, 2002 at 19:38:16 ---------------------------------------------------------------------------
Email: amostaghimi-at-onyx-pharm.com Name: ALI MOSTAGHIMI
Organization: ONYX PHARMACEUTICALS
Education: Graduate College
Location: RICHMOND, CA 94806
Question: I am interested to find out about EM technology that can show images of human adenovirus particles that my have extraneous pieces of DNA stock to them. Do you think it is possible to produce images with these two requirements? Your reply is appreciated.
Best Regards,
Ali Mostaghimi QC manager, project services Onyx Pharmaceuticals 3031 Research Drive Richmond, CA 94806 Phone: 510-243-3697
An advanced course on polarized light microscopy which will cover the following topics:
The nature of polarized light
The origin and interpretation of interference colors
Birefringence and crystal orientation
The Indicatrix
Compensation and variable compensators
Interference figures and their interpretation
The workshop will consist of four Consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy. The course instructors include Jan Hinsch of Leica, Inc., Mary McCann of McCann Imaging, John Reffner of SensIR and N.Y.M.S. Instructor Don O'Leary.
WHEN: April 26, May 3, 10 &17, 2003, from 10 A.M. to 4 P.M.
COST: $340 for N.Y.M.S. members, $370 for non-members (includes membership) Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use.
HOW: Register using the form below. Limited to the first 12 registrants.
Return form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.
FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 e-mail donoleary-at-att.net
} I got my neurologist a used single power stereo microscope that his 5 year } old loves. Some think that monocular work better for young kids because } there is less to adjust. When you find them monocular low powered scope that } have inverting prisms sell for a song.
You've got it slightly wrong, Gordon. One problem with a binocular "dissecting" scope for young children is that the minimum interocular spacing usually is too wide for their eyes. A second is that almost a fifth of young kids have some problem with stereo vision that makes use of a binoc frustrating. A third problem is that they occasionally drop things, which throws out the ocular alignment. A fourth is that they cost a lot more. 20x monocs are wonderful beginner scopes for children, and good ones are available for $70 or less. Where? Look at the MICRO website (URL below) and/or Email me. } Caroline
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
I am trying to build up an image analysis system with a metallographic microscope. I have a few microscopes except a CCD camera. Would you please recommend ccd cameras with low ~ medium price range. Thank you very much in advance.
In a message dated 12/4/2002 8:00:47 PM Eastern Standard Time, ramirez-at-amnh.org writes:
} Does anyone knows of a free or accessible software to compose in-focus 2-D } images from several images in different focal planes? } Martin
Not aware of any free, but the image processing tool kit does include that capability (plus many others) and is relatively inexpensive. see http://reindeergraphics.com
I know I am a bit late but have you seen this suggestion?
Randy what you are describing is exactly the symptom of poor quality filaments, I even have pictures to demonstrate what you describe within our maintenance CD course.
} From time to time the wire used to manufacture filaments is not up to standard, it contains a level of garbage; no criticism of the manufacturer of the filaments more a criticism of the wire maker! In these circumstances the filaments thin normally but a good deal quicker and with far more of the evaporated "materials". Never panic with small blobs on the filament break as if an operator is unlucky this can happen to the most experienced of us.
As you know I have worked with several electron microscope manufacturers and every few years or so this type of problem crops up.
My advice to EM operators is NEVER use a complete box of filaments that have served you well; always save two. Then in the situation that you now have you can pop in one of a good box to prove you have a problem, or that the new box of filaments is of a poor quality.
So how should you proceed - 1. You need to run another filament from the new box with great care, keep an eye on the operators to make sure you do not have an oversaturation problem with one of them. 2. If the problem is repeated replace with a filament from a good box. 3. If the problem continues you probably have a leak in the gun area, forget what the gauges say unless they are actually in the gun area. 4. If the problem went away you have proven the new filaments to be at error, return them to the supplier with an explanation of your problem
Other areas of investigation if this does not solve your problem are-
1. The colour of the ceramic - yellow/brown suggests a vacuum leak 2. A smelly gun chamber - smells of oil/ozone - suggests a vacuum leak 3. Normal filament break but THE WHOLE OF THE FILAMENT HAS THINNED, not just the tip - gas attack - suggests a vacuum leak
Hope this helps, maybe we could privately have a chat about saturation?
Regards
Steve Chapman Senior Consultant Protrain EM Training and Consultancy World Wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 www.emcourses.com
} I am trying to build up an image analysis system with a metallographic } microscope. I have a few microscopes except a CCD camera. Would you } please recommend ccd cameras with low ~ medium price range. Thank you } very much in advance.
I've had quite a lot of success interfacing cheap (US$200) CCTV cameras to microscopes. If the eyepiece is detachable from the scope, you can just offer up the camera without lens. If you can't remove the eyepiece, you need to use a lens on the camera, focussed to infinity, and as close as possible to the eyepiece.
You can feed the composite video signal from the CCD camera to a TV (AV input), or to a video capture card (about US$100) in a computer. That way you can capture, store, and print the images.
Quality is OK for enlargement up to about postcard size.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Bummer. I've never dealt with Energy Beam Sciences (not "EM sciences" -- a typo?). Just the GKM knifemaker, and to boot, this was before the knifemaker's name was changed to "GKM". The ones I knew were Sorvalls. Another company bought the product, I guess, and now a good product is down the tubes?
I completely agree about testing equipment before buying. More importantly, test the company selling the product.
Phil
} Phil, I do agree that GKM knifemaker is good. Unfortunately I have } extremely bad experience with EM Sciences. When I am shopping for } equipment, my rule is that I have to see how equipment works before } I buy it (demo or exibition). Like car, you have to try it first. I } was asking EM Sciences for the demo. They refused. Finally after } discussion on the high level they did agree to sent to me instrument } for demo and I'll buy it if I like it... I DID sign a special 5 } pages Contract (never did before), they supposed to sent instrument } after that. Now it's about a year as they DO promise to send the } instrument. When I called them a month later after signing the } Contract - it was a panic on the other end - they were urgently } assembling the instrument (they did not have any at hand). So, they } were lay when sign the Contract (there were exact dates on the } Contract for shipping). I told them, OK, I'll wait. So, I am } waiting and waiting... until now. I do have all paperwork from that } case to verify that the story is true. It looks to me that Leica is } only a possibility for majority of us. I really don't like old RMC } knifemaker, but newer try the current model. Personally I did not } try 10 mm on Leica, but 8 mm knifes were really good, no complains. } I don't have Leica instrument, I sort of wait for response from EB } Sciences... meantime using 10 mm W-carbide knifes from DDK. By the } way, EB Sciences was trying to sell to me Leica instead GKM, so they } definitely have some problems with GKM. I think this is a purpose of } our ListServer: to share experience, bad and good. I wish everyone } a great Holiday Season and according Russian tradition, I wish, all } your wishes (at least a few of them) become true in the coming New } Year. Sergey } } At 05:59 AM 12/6/2002, you wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I think, some of the features you listed are available from Win2000 or WinXP (if you are using a PC, that is). You can set your folders to preview images as thumbnails (XP does a number of formats), and you can certainly copy and move them around. XP also has a "filmstrip" mode, where you see one image large and the others as smaller thumbnails along the bottom.
However, I think you are more looking for an image archive that you can search and where you can provide additional information that you can use later for finding images. We have that as part of our analySIS software, but there are other applications out there as well. A solution like this usually requires a bit more up-front work (you have to define the keywords and key fields), but it will be much more efficient in the long run. If you check for solutions like this, make sure you don't have to pay too much for "reader" software (ours is free), and perhaps options for internet access to the archive. Finally, although you are in Canada and this may only apply to US companies, make sure that your archive or data base software can be made rule 11 compliant if you have to deal with the FDA.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca] Sent: Tuesday, December 03, 2002 11:32 AM To: Microscopy-at-sparc5.microscopy.com
Fellow microscopists :
I recently collected a lot of images at a single sitting, each image seemingly better than the previous one, definitely on a roll. However, the next day I laboriously had to review a lot of images of similar data and choose the best ones.
What software are people using to browse through a lot of images, as thumbnails, easily change their names, perhaps add annotations, move selected ones into new folders and create a catalogue. It should also be able to recognize and load confocal image stacks.
Thank you Judy
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 8Queen 30 Bond St. Toronto, ON M5B 1W8 Canada
Probably the lowest price solution would be Kodak MDS 100 (I bought one for around $100 a while ago). However, it doesn't work with all the microscopes and you might have to buy a video relay lens (adapter) for the camera, which would cost you up to $200, at least. However, the camera I bought didn't "impress" me with the image quality, particularly color wise. Therefore, sometimes I use a color CCD camera (1/3"), a video lens and a video capture device (it may be a computer card). The quality is just perfect and the price is about the same, since it's possible to buy a relatively good CCD camera for around $100.
Vladimir Igoshev, Ph.D.
Toronto, Canada
----- Original Message ----- } From: "Elliot Kyung-Ho Lee" {khlee-at-ybust.edu.cn} To: {Microscopy-at-sparc5.microscopy.com} Sent: Sunday, December 08, 2002 12:50 AM
I wrote some IDL code (http://www.rsinc.com) that does that. It is just code, and there is no graphical user interface (yet). I create an array which classifies the pixel of each image with a score, and then extract the 'best scoring pixel' from each of the input images in order to create an output image. The way that score is obtained must relate to your 'feature size'; technically, I use the difference of the blurred image (or spot area) to the original image for each one of the images in order to calculate that score.
Example image: http://www.swisswuff.ch/pnphoenix721/html/modules.php?op=modload&name=News&file=article&sid=5&mode=thread&order=0&thold=0
I am not sure if that is what you are after.
Wolf Schweitzer
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } In a message dated 12/4/2002 8:00:47 PM Eastern Standard Time, } ramirez-at-amnh.org writes: } } } Does anyone knows of a free or accessible software to compose in-focus 2-D } } images from several images in different focal planes? } } Martin } } Not aware of any free, but the image processing tool kit does } include that capability (plus many others) and is relatively } inexpensive. see http://reindeergraphics.com
I am trying to locate trace metals in tissues. I have been cutting dry sections since I cannot use water. Sectioning on water could move or dissolve the trace metals I am after.
Could you recommend any references or techniques about alternatives to using water for sectioning ?
Thanks for any help
L. Berry
..................................................... Lorraine Berry RBINS Department of Invertebrates Rue Vautier 29 Bruxelles Lorraine.berry-at-naturalsciences.be .....................................................
I've heard that there is a microscope adaptor for the Nikon CoolPix line of cameras. Some of the CoolPix model are upper-end consumer cameras, which run up to about $1000. Sorry, I don't know the details on the adaptor, but try an internet search. --------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Division of Natural Sciences Purchase College State University of New York Purchase, NY 10577 --------------------------------------- Ofc. Tel: 914-251-6659 Ofc. Fax: 914-251-6635 E-mail: jfactor-at-purvid.ns.purchase.edu ---------------------------------------
Ritchie Sims wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am trying to build up an image analysis system with a metallographic } } microscope. I have a few microscopes except a CCD camera. Would you } } please recommend ccd cameras with low ~ medium price range. Thank you } } very much in advance. } } I've had quite a lot of success interfacing cheap (US$200) CCTV cameras to } microscopes. If the eyepiece is detachable from the scope, you can just offer } up the camera without lens. If you can't remove the eyepiece, you need to use } a lens on the camera, focussed to infinity, and as close as possible to the } eyepiece. } } You can feed the composite video signal from the CCD camera to a TV (AV } input), or to a video capture card (about US$100) in a computer. That way you } can capture, store, and print the images. } } Quality is OK for enlargement up to about postcard size. } } cheers } } rtch } } Ritchie Sims Phone : 64 9 3737599 ext 7713 } Department of Geology Fax : 64 9 3737435 } The University of Auckland email : r.sims-at-auckland.ac.nz } Private Bag 92019 } Auckland } New Zealand
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Yes, it was typo, the company's correct name is Energy Beam Sciences. Sorry. Sergey
At 02:23 PM 12/8/02 -0600, you wrote: } Sergey, } } Bummer. I've never dealt with Energy Beam Sciences (not "EM sciences" -- a } typo?). Just the GKM knifemaker, and to boot, this was before the } knifemaker's name was changed to "GKM". The ones I knew were Sorvalls. } Another company bought the product, I guess, and now a good product is } down the tubes? } } I completely agree about testing equipment before buying. More } importantly, test the company selling the product. } } Phil } } } Phil, I do agree that GKM knifemaker is good. Unfortunately I have } } extremely bad experience with EM Sciences. When I am shopping for } } equipment, my rule is that I have to see how equipment works before I buy } } it (demo or exibition). Like car, you have to try it first. I was asking } } EM Sciences for the demo. They refused. Finally after discussion on the } } high level they did agree to sent to me instrument for demo and I'll buy } } it if I like it... I DID sign a special 5 pages Contract (never did } } before), they supposed to sent instrument after that. Now it's about a } } year as they DO promise to send the instrument. When I called them a } } month later after signing the Contract - it was a panic on the other end } } - they were urgently assembling the instrument (they did not have any at } } hand). So, they were lay when sign the Contract (there were exact dates } } on the Contract for shipping). I told them, OK, I'll wait. So, I am } } waiting and waiting... until now. I do have all paperwork from that case } } to verify that the story is true. It looks to me that Leica is only a } } possibility for majority of us. I really don't like old RMC knifemaker, } } but newer try the current model. Personally I did not try 10 mm on Leica, } } but 8 mm knifes were really good, no complains. I don't have Leica } } instrument, I sort of wait for response from EB Sciences... meantime } } using 10 mm W-carbide knifes from DDK. By the way, EB Sciences was } } trying to sell to me Leica instead GKM, so they definitely have some } } problems with GKM. I think this is a purpose of our ListServer: to share } } experience, bad and good. I wish everyone a great Holiday Season and } } according Russian tradition, I wish, all your wishes (at least a few of } } them) become true in the coming New Year. Sergey } } } } At 05:59 AM 12/6/2002, you wrote: } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
We have Coolpix adapters available for most microscopes for mounting in lieu of the eyepieces or through a trinocular port. Price range is from $300 - $500 depending on the clamps needed for your particular setup.
1.) One nice thing about our Coolpix system is that the optics system is universal as far as the microscope is concerned. We use the same optics no matter which scope you are using - you would just need a different clamp to mount on various models of microscopes.
2.) If you would rather mount through the eyepieces of the scope, our adapters will drop INTO the eyepiece receivers on the microscope and NOT grip around them as some of the digital camera adapters on the market. This is a much more stable way of doing things so you don't wind up with a $700 camera falling off your scope.
3.) Our adapters also have correct optics and magnification for the Coolpix series cameras built in so that you get the full zoom range of the camera with no vignetting. There is neither chromatic aberration or spherical aberration with our adapters. AND we stand behind them with a 30 day money back guarantee.
*******************Easier to use************************************
4.) You don't have to screw it onto the microscopes - just leave the adapter attached to your camera and all you'll have to do is pull the eyepiece on your scope and drop the camera/adapter assembly in - rather than trying to get a bunch of screws tightened on the eyepieces. If you are going through the eyepieces, this makes it a lot easier to take the camera off and on the microscope.
5.) It's less bulky and much cleaner than competing adapters so it won't get in the way if you want to look in the other eyepiece while the camera is attached to the microscope.
6.) You don't have to worry about getting the camera the correct distance from the ocular to have the camera parfocal with the microscope because our adapter slides into the eyepiece tube rather than clamping around the eyepiece and having the camera at the incorrect optical distance from the eyepiece.
7.) You don't have to worry about the magnification of the eyepieces in your microscopes - our adapter has the correct magnification for the Coolpix series cameras ALL the time.
Please feel free to call me or email me if you would like to discuss your application in greater detail.
Thanks & Happy Holidays! Jim Haley
****************************** Jim Haley Applications Engineer MVIA, Inc. 125 Sherwood Drive Monaca, PA 15061 voice: (724) 728-7493 fax: (412) 291-1709 e-mail: haley-at-mvia.com webpage: http://www.mvia.com ******************************
atcsem wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I am looking into getting digital camera and came across Nikon Coolpix } microscope kit } } http://www.ipsimaging.com/products/cameras/coolpixkit.htm } A little bit pricey! } } Regards, } Pavel } } ----- Original Message ----- } } From: "jan factor" {jfactor-at-purvid.ns.purchase.edu} } To: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } Cc: "Elliot Kyung-Ho Lee" {khlee-at-ybust.edu.cn} ; {} } Sent: Monday, December 09, 2002 11:41 AM } Subject: Re: LM: CCD camera for LM } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I've heard that there is a microscope adaptor for the Nikon CoolPix line } of } } cameras. Some of the CoolPix model are upper-end consumer cameras, which } run up } } to about $1000. Sorry, I don't know the details on the adaptor, but try an } } internet search. } } --------------------------------------- } } Jan Robert Factor, Ph.D. } } Professor of Biology } } --------------------------------------- } } Division of Natural Sciences } } Purchase College } } State University of New York } } Purchase, NY 10577 } } --------------------------------------- } } Ofc. Tel: 914-251-6659 } } Ofc. Fax: 914-251-6635 } } E-mail: jfactor-at-purvid.ns.purchase.edu } } --------------------------------------- } } } } } } Ritchie Sims wrote: } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } I am trying to build up an image analysis system with a metallographic } } } } microscope. I have a few microscopes except a CCD camera. Would you } } } } please recommend ccd cameras with low ~ medium price range. Thank you } } } } very much in advance. } } } } } } I've had quite a lot of success interfacing cheap (US$200) CCTV cameras } to } } } microscopes. If the eyepiece is detachable from the scope, you can just } offer } } } up the camera without lens. If you can't remove the eyepiece, you need } to use } } } a lens on the camera, focussed to infinity, and as close as possible to } the } } } eyepiece. } } } } } } You can feed the composite video signal from the CCD camera to a TV (AV } } } input), or to a video capture card (about US$100) in a computer. That } way you } } } can capture, store, and print the images. } } } } } } Quality is OK for enlargement up to about postcard size. } } } } } } cheers } } } } } } rtch } } } } } } Ritchie Sims Phone : 64 9 3737599 ext 7713 } } } Department of Geology Fax : 64 9 3737435 } } } The University of Auckland email : r.sims-at-auckland.ac.nz } } } Private Bag 92019 } } } Auckland } } } New Zealand } } } } -- } } } } } }
In my original message concerning GKM knifemaker from Energy Beam Sciences I mistakenly mentioned "EM Sciences" instead "EB Sciences". I deeply apologies for that mistake. Energy Beam Sciences is current manufacturer (?) of the GKM knifemakers and all my message was about that particular company. I never had any problems with "EM Sciences". I am really sorry for mistake. Sergey
At 02:23 PM 12/8/02 -0600, you wrote: } Sergey, } } Bummer. I've never dealt with Energy Beam Sciences (not "EM sciences" -- a } typo?). Just the GKM knifemaker, and to boot, this was before the } knifemaker's name was changed to "GKM". The ones I knew were Sorvalls. } Another company bought the product, I guess, and now a good product is } down the tubes? } } I completely agree about testing equipment before buying. More } importantly, test the company selling the product. } } Phil } } } Phil, I do agree that GKM knifemaker is good. Unfortunately I have } } extremely bad experience with EM Sciences. When I am shopping for } } equipment, my rule is that I have to see how equipment works before I buy } } it (demo or exibition). Like car, you have to try it first. I was asking } } EM Sciences for the demo. They refused. Finally after discussion on the } } high level they did agree to sent to me instrument for demo and I'll buy } } it if I like it... I DID sign a special 5 pages Contract (never did } } before), they supposed to sent instrument after that. Now it's about a } } year as they DO promise to send the instrument. When I called them a } } month later after signing the Contract - it was a panic on the other end } } - they were urgently assembling the instrument (they did not have any at } } hand). So, they were lay when sign the Contract (there were exact dates } } on the Contract for shipping). I told them, OK, I'll wait. So, I am } } waiting and waiting... until now. I do have all paperwork from that case } } to verify that the story is true. It looks to me that Leica is only a } } possibility for majority of us. I really don't like old RMC knifemaker, } } but newer try the current model. Personally I did not try 10 mm on Leica, } } but 8 mm knifes were really good, no complains. I don't have Leica } } instrument, I sort of wait for response from EB Sciences... meantime } } using 10 mm W-carbide knifes from DDK. By the way, EB Sciences was } } trying to sell to me Leica instead GKM, so they definitely have some } } problems with GKM. I think this is a purpose of our ListServer: to share } } experience, bad and good. I wish everyone a great Holiday Season and } } according Russian tradition, I wish, all your wishes (at least a few of } } them) become true in the coming New Year. Sergey } } } } At 05:59 AM 12/6/2002, you wrote: } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} Date: Mon, 09 Dec 2002 17:05:09 -0800 } To: SGKCCK-at-aol.com } From: Sergey Ryazantsev {sryazant-at-ucla.edu} } Subject: Re: GKM } } I apologize } I already post statement on ListServer that I mentioned your company } wrongly. I really sorry. Sincerely yours, Dr. Sergey Ryazantsev } } At 10:12 AM 12/7/2002, you wrote: } } This is Stacie kirsch the owner of EM Sciences (Electron Mi8croscopy } } Sciences). You are using our name in place of EB Sciences and we have } } nothing to do with Glass knife makers. I would appreciate you retract } } and make the correction so everyone understands you meant EB Sciences and } } not EM Sciences. } } I would really appreciate it. } } Thanks } } Stacie Kirsch } } President } } _____________________________________ } } Sergey Ryazantsev Ph. D. } Electron Microscopy } UCLA School of Medicine } Department of Biological Chemistry } Box 951737 } Los Angeles, CA 90095-1737 } } Phone: (310) 825-1144 } FAX (departmental): (310) 206-5272 } mailto:sryazant-at-ucla.edu }
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
The website for EM Science is at URL http://www.emscience.com/
The ownership is described somewhat differently. You can see for yourself.
I was under the impression that they were part of the EM Industries Group, an Affiliate of Merck KGaA out of Darmstadt, Germany. That is also according to the website.
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
} Date: Mon, 09 Dec 2002 17:05:09 -0800 } To: SGKCCK-at-aol.com } From: Sergey Ryazantsev {sryazant-at-ucla.edu} } Subject: Re: GKM } } I apologize } I already post statement on ListServer that I mentioned your company } wrongly. I really sorry. Sincerely yours, Dr. Sergey Ryazantsev } } At 10:12 AM 12/7/2002, you wrote: } } This is Stacie kirsch the owner of EM Sciences (Electron Mi8croscopy } } Sciences). You are using our name in place of EB Sciences and we have } } nothing to do with Glass knife makers. I would appreciate you retract } } and make the correction so everyone understands you meant EB Sciences and } } not EM Sciences. } } I would really appreciate it. } } Thanks } } Stacie Kirsch } } President } } _____________________________________ } } Sergey Ryazantsev Ph. D. } Electron Microscopy } UCLA School of Medicine } Department of Biological Chemistry } Box 951737 } Los Angeles, CA 90095-1737 } } Phone: (310) 825-1144 } FAX (departmental): (310) 206-5272 } mailto:sryazant-at-ucla.edu }
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
I have an old LKB pyramitome and some old Sorvall MT2 and MT7 chucks (with the threaded bottom). There used to be adapters out there that you screwed onto the threaded bit that allowed you to use the Sorval chucks in things like my LKB.
Does any one know where I can get one?
Thanks oodles & poodles in advance!
Paula :-)
Paula Sicurello George Washington Univ. Medical Center Dept. of Pathology, Ross Hall rm 505 Electron Microscope Lab 2300 Eye St. Washington, DC 20037 202-994-2930 phone 202-994-2518 fax
Nikon has an adapter for the Coolpix cameras. The price was about $600 two years ago. This adapter works in the eyepiece tube or will attach to a C-mount adapter.
You can also make your own adapter with a 10X eyepiece with 28 mm threads to match the threads on the lens of the Coolpix. Some of the new cheaper Coolpix cameras do not have the threaded lens, so check for that before buying a camera for this application.
We have used this combination for a couple of years on various microscope with pretty good results. One major problem with microscopy is the zoom setting on the camera must be set to a fixed setting to get accurate magnification, and there is no way to see what the zoom setting is when setting up the camera. -- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
I'd recommend MVIA's Coolpix adapters. We have been using them in the lab for a few months now.
We had tried unsuccessfully to get good images using our Coolpix 995 camera with an adapter from a different vendor. This summer we decided to try the adapters from MVIA. The adapters have worked great for us and we can now get good images from the camera.
Dear all, Are other people doing particle size measurement from TEM images, and if so how? The particles are small metal catalyst particles on carbon supports. One problem is that automatic image processing could fail because of the background contrast ripples from the carbon. At the moment the student is simply measuring everything with a ruler, and this takes ages, of course.
Secondly, does anyone have a good reference on particle size distribution? Specifically, we would like to know how distributions are best represented statistically, particularly when they are non-normal (short of just showing a diagram for each one).
Best wishes -- Ian MacLaren Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/
N.B.: EM Science is not EM Sciences, necessarily. That is, EM (E. Merck) Science, an affiliate of Merck KGaA out of Darmstadt, Germany, located in Gibbstown, NJ, is not EM (Electron Microscopy) Sciences, aka EMS, of Fort Washington, PA. Since we often order from both companies, I ALWAYS specify Electron Microscopy Sciences or EM Science instead of EMS, especially since I'm also a registered EMT volunteering in our EMS department, which has nothing to do with EM. I won't even mention the number of times our orders are delivered to the EM (Emergency Medicine) Department instead of our EM (Electron Microscopy) Department. Clear now?! (sorry Nester) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W. Wiggins, Supervisor Cannon Electron Microscopy Lab Carolinas Medical Center P.O. Box 32861; Charlotte, NC 28232-2861 Ship to: 1000 Blythe Blvd; Charlotte, NC 28203 Ofc:704-355-1267 ; Lab:704-355-7220 ; Fax 704-355-0589 E-mail: Winston.Wiggins-at-CarolinasHealthCare.org {mailto:WWiggins-at-Carolinas.org} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-----Original Message----- } From: Garber, Charles A. [SMTP:cgarber-at-2spi.com] {mailto:[SMTP:cgarber-at-2spi.com]} Sent: Monday, December 09, 2002 11:38 PM To: MICROSCOPY BB
Dear Colleagues,
I wish to thank all of you for the timely information regarding the facility available at the DC-Baltimore area. I passed the information to the friend along with my own appreciation.
Do you have to adjust, crop images to get the correct magnification?
Pavel
----- Original Message ----- } From: {Mortro-at-aol.com} To: {haley-at-mvia.com} ; {atcsem-at-earthlink.net} Microscopy-at-sparc5.microscopy.com Cc: {jfactor-at-purvid.ns.purchase.edu} ; {} ; {atcsem-at-earthlink.net} Sent: Tuesday, December 10, 2002 11:08 AM
We have debated a Coolpix type solution, but decided against it as too much theft potential in a multiuser lab. Anyone come up with a solution to this dilemma? Dave
On Tuesday, December 10, 2002, at 10:31 AM, Larry Hanke wrote:
} ----------------------------------------------------------------------- } - } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } . } } } Nikon has an adapter for the Coolpix cameras. The price was } about $600 two years ago. This adapter works in the eyepiece } tube or will attach to a C-mount adapter. } } You can also make your own adapter with a 10X eyepiece with } 28 mm threads to match the threads on the lens of the } Coolpix. Some of the new cheaper Coolpix cameras do not have } the threaded lens, so check for that before buying a camera } for this application. } } We have used this combination for a couple of years on } various microscope with pretty good results. One major } problem with microscopy is the zoom setting on the camera } must be set to a fixed setting to get accurate } magnification, and there is no way to see what the zoom } setting is when setting up the camera. } -- } Larry D. Hanke, P.E. } Materials Evaluation and Engineering, Inc. } Practical Solutions Through Technology and Innovation } http://www.mee-inc.com (763) 449-8870 } } } } Dr. David Knecht Department of Molecular and Cell Biology U-125 75 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
The '30 day warranty' I spoke of is not the actual warranty period. It is a trial period and is certainly enough time to test the adapter out and verify its quality and determine if the adapter is satisfactory. We offer a full refund less freight for that period - up to 30 days after receipt of the adapters.
The actual warranty period for the adapter is one year for any defects in workmanship, or if it breaks. Up to one year, we will exchange/repair any adapters that break (as long as its not due to abuse).
I can tell you that in the 2 years we have been selling the adapters we have shipped a couple hundred adapters. Out of all these, we have not had even 1 inquiry by anyone who wanted to return the adapter for any reason!
Thanks & Happy Holidays! Jim Haley
****************************** Jim Haley Applications Engineer MVIA, Inc. 125 Sherwood Drive Monaca, PA 15061 voice: (724) 728-7493 fax: (412) 291-1709 e-mail: haley-at-mvia.com webpage: http://www.mvia.com ******************************
"Foran, David A" wrote: } } "AND we stand behind them with a 30 day money back guarantee." } } This is more like stooping behind your product. 30 days does not give me } much confidence in the product. } } -----Original Message----- } From: Jim Haley [mailto:haley-at-mvia.com] } Sent: Monday, December 09, 2002 4:57 PM } To: atcsem } Cc: jan factor; Microscopy-at-sparc5.microscopy.com; atcsem-at-earthlink.net } Subject: Re: LM: CCD camera for LM } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Chain it! Just a joke. I think there is a time when we need to start trusting coworkers.
Pavel
----- Original Message ----- } From: "David Knecht" {knecht-at-uconn.edu} To: "microscopy" {microscopy-at-sparc5.microscopy.com} Sent: Tuesday, December 10, 2002 12:52 PM
1. Whether digitally or in dark room try to find right contrast for your images. Carbon film, even folded, should have lower darkness than metal particles. 2. If this fails, try semiautomatic analysis, where you can eliminate unwonted features from each field of view. 3. If you have access to a SEM and your particles are big enough, then the easiest way is to analyze images obtained with a backscattered detector.
Regards,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 3127 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de] } Sent: Tuesday, December 10, 2002 10:22 AM } To: Microscopy } Subject: TEM Particle size measurement } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Dear all, } Are other people doing particle size measurement from TEM } images, and if } so how? The particles are small metal catalyst particles on carbon } supports. One problem is that automatic image processing could fail } because of the background contrast ripples from the carbon. At the } moment the student is simply measuring everything with a } ruler, and this } takes ages, of course. } } Secondly, does anyone have a good reference on particle size } distribution? Specifically, we would like to know how } distributions are } best represented statistically, particularly when they are non-normal } (short of just showing a diagram for each one). } } Best wishes } -- } Ian MacLaren } Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany } ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/ } } }
Can you remove the background through a shading correction? If your background variation is very different from your particles (background low frequency fluctuations on a larger scale than the particles), I would recommend to try unsharp masking. Take the image, run an NxN filter on the image, then subtract from original image. You can play with the filter size for best results.
As for display: automatically detect the particles after the correction above and measure the parameter you want to display. You will then have to define a classification, and then a simple histogram of the results should give you the diagram you want. Other than that you could evaluate higher order statistical parameters from the results (such as kurtosis), and specify those. Our software has those as standard statistical parameters, and I would assume other software does as well.
If you send me an image, I can run that here and send you the results.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de] Sent: Tuesday, December 10, 2002 9:22 AM To: Microscopy
Dear all, Are other people doing particle size measurement from TEM images, and if so how? The particles are small metal catalyst particles on carbon supports. One problem is that automatic image processing could fail because of the background contrast ripples from the carbon. At the moment the student is simply measuring everything with a ruler, and this takes ages, of course.
Secondly, does anyone have a good reference on particle size distribution? Specifically, we would like to know how distributions are best represented statistically, particularly when they are non-normal (short of just showing a diagram for each one).
Best wishes -- Ian MacLaren Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/
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No, we don't have to crop. On some of the scopes, we adjust the zoom properly on our camera first before taking pictures but it's always the same for each scope.
We have been using the adaptor Jim sells along with two other microscope adaptors he supplied for about a year now. We are very pleased with them. We can now put the Coolpix on our hardness tester, tissue culture microscope, metallurgical microscope, the dissecting microscope and our compound microscope. Fantastic.
Ron L
-----Original Message----- } From: Jim Haley [mailto:haley-at-mvia.com] Sent: Tuesday, December 10, 2002 3:08 PM To: Foran, David A; Microscopy-at-sparc5.microscopy.com
David,
Good point. My description was inaccurate.
The '30 day warranty' I spoke of is not the actual warranty period. It is a trial period and is certainly enough time to test the adapter out and verify its quality and determine if the adapter is satisfactory. We offer a full refund less freight for that period - up to 30 days after receipt of the adapters.
The actual warranty period for the adapter is one year for any defects in workmanship, or if it breaks. Up to one year, we will exchange/repair any adapters that break (as long as its not due to abuse).
I can tell you that in the 2 years we have been selling the adapters we have shipped a couple hundred adapters. Out of all these, we have not had even 1 inquiry by anyone who wanted to return the adapter for any reason!
Thanks & Happy Holidays! Jim Haley
****************************** Jim Haley Applications Engineer MVIA, Inc. 125 Sherwood Drive Monaca, PA 15061 voice: (724) 728-7493 fax: (412) 291-1709 e-mail: haley-at-mvia.com webpage: http://www.mvia.com ******************************
"Foran, David A" wrote: } } "AND we stand behind them with a 30 day money back guarantee." } } This is more like stooping behind your product. 30 days does not give me } much confidence in the product. } } -----Original Message----- } From: Jim Haley [mailto:haley-at-mvia.com] } Sent: Monday, December 09, 2002 4:57 PM } To: atcsem } Cc: jan factor; Microscopy-at-sparc5.microscopy.com; atcsem-at-earthlink.net } Subject: Re: LM: CCD camera for LM } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
We keep ours in a locked drawer when not in use. Only the technicians have the combination. Of course, locks only keep out honest people.
Ron L
-----Original Message----- } From: atcsem [mailto:atcsem-at-earthlink.net] Sent: Tuesday, December 10, 2002 2:30 PM To: David Knecht Cc: microscopy-at-sparc5.microscopy.com
Chain it! Just a joke. I think there is a time when we need to start trusting coworkers.
Pavel
----- Original Message ----- } From: "David Knecht" {knecht-at-uconn.edu} To: "microscopy" {microscopy-at-sparc5.microscopy.com} Sent: Tuesday, December 10, 2002 12:52 PM
Ian The mean, standard deviation, standard error of the mean, skewness and kurtosis would provide a good description of the distribution, the latter two being measures of two kinds of departure from normality. All of these can be calculated using Microsoft Excel (Do Insert _ Function_ Statistical _ AVERAGE, STDEV, STDEV/SQRT(N),SKEW, KURT, ) If you want a diagram then a frequency distribution plot can be calculated (Do Tools_ Data Analysis_ HISTOGRAM). These functions are also fairly automatic in most statistical analysis packages such as SigmaStat, SPSS, Minitab, some of which will plot a normal distribution curve on top of the histogram. Chris
} From: Ian MacLaren [mailto:maclaren-at-tu-darmstadt.de] } Sent: Tuesday, December 10, 2002 9:22 AM } To: Microscopy } Subject: TEM Particle size measurement } } } -------------------------------------------------------------------- ---- } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------- ---. } } } Dear all, } Are other people doing particle size measurement from TEM images, and if } so how? The particles are small metal catalyst particles on carbon } supports. One problem is that automatic image processing could fail } because of the background contrast ripples from the carbon. At the } moment the student is simply measuring everything with a ruler, and this } takes ages, of course. } } Secondly, does anyone have a good reference on particle size } distribution? Specifically, we would like to know how distributions are } best represented statistically, particularly when they are non-normal } (short of just showing a diagram for each one). } } Best wishes } -- } Ian MacLaren } Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany } ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/ } }
List members, We have an old Hummer coater that was marketed by the Technics Company. I would like to find out if this company is still in business and, if so, where they are now located. Also, who is presently handling the Hummer coater line or who has been in the recent past. I know there were a number of other models produced since ours was purchased in the early 80's. Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building West Lafayette, IN 47907
List: Does anyone have experience with Nikons new low end professional digital SLR offering, model D100? It uses a standard Nikon SLR body and mount so most of their lenses fit and auto features will work with many of the lenses (AF & D type that we use). It is a 6 mega pixel camera listing for ~$2,000 (body only). Since we currently use Nikon SLR camera's on our OM systems and have several Nikon lenses for copy stand work this seems like a very good fit. Considering all of the costs associated with purchasing and mounting fixed lens consumer camera's onto microscopes it appears to be a viable option? True the D100 costs are slightly higher however the benefits are significant, high resolution images, selectable lens ranges, improved lens quality, versatile camera software, and I am assuming it uses standard microscope mounting hardware found in many labs.
What have I missed? Are there pitfalls associated with this camera? Since this is a newer camera model I can not confirm some of the microscope mounting information. Please correct any errors or misinformation above. Any additional input or insight would be appreciated.
Sincerely, jr
John Robson Boehringer Ingelheim Pharmaceuticals, Inc. PO Box 368 900 Ridgebury Rd Ridgefield, CT 06877
Phone (203)798-5640 Fax (203)798-5698
e-mail jrobson-at-RDG.boehringer-ingelheim.com
-----Original Message----- } From: "Mortro-at-aol.com"-at-sparc5.microscopy.com [mailto:"Mortro-at-aol.com"-at-sparc5.microscopy.com] Sent: Tuesday, December 10, 2002 11:08 AM To: haley-at-mvia.com; atcsem-at-earthlink.net Cc: jfactor-at-purvid.ns.purchase.edu; Microscopy-at-sparc5.microscopy.com; atcsem-at-earthlink.net
I'd recommend MVIA's Coolpix adapters. We have been using them in the lab for a few months now.
We had tried unsuccessfully to get good images using our Coolpix 995 camera with an adapter from a different vendor. This summer we decided to try the adapters from MVIA. The adapters have worked great for us and we can now get good images from the camera.
} Chain it! } Just a joke. I think there is a time when we need to start trusting } coworkers.
Unfortunately it is not just our coworkers. Many labs have students, janitorial workers, maintenance staff and casual passer-bys who need only a moment of opportunity to steal something. As soon as the microscope manufacturers started putting consumer cameras on microscopes we started losing cameras to theft. This was never a problem with the dedicated microscope cameras which could not be used for other purposes. A researcher at our University just had a digital consumer camera stolen from his microscope along with irreplaceable data stored on the memory card. Our pathology department had an entire video microscope system stolen from the conference room by a student. How it was found in his locker months later by the police I don't know. I suspect he had been involved in other thefts. This department now has anti-theft devices on all of their microscopes. These are either glued on pads with a security cable and lock or an eyebolt attached to the microscope body with a cable and lock. I have mounted a small diameter cable on a Coolpix camera attached to the neck strap attachment point. The tripod socket could also be used as an attachment point. The cable is attached to the microscope with a lock and is long enough that the camera can be moved between several scopes.
If someone is determined to steal something there is almost nothing we can do to stop them, and if the security measures become too onerous people will stop using them. It has been our experience though that it only takes a minor deterrent to prevent most thefts.
David Burton Optical Specialist University of Washington
} ... } The '30 day warranty' [...] is certainly enough time } to test the adapter out and verify its quality and } determine if the adapter is satisfactory. ...
Before we order, or request a trial, how do we best determine what type of 'C-mount' is required. When I looked into this more than a year ago, a 1X would have been best for a 9xx Coolpix and its CCD. Is there a formula for determining the mag factor of the C-mount adapter relative to the size of the CCD in the camera???
Also ... is the adapter different with regard to older Nikon 9xx and newer 5xxx cameras??
cheerios ... shAf :o) Avalon Peninsula, Newfoundland www.micro-investigations.com (in progress)
Hello, Does anyone know of a fluorescent dye that would specifically tag/stain silicone oil droplets in complex mixtures or suspensions? Thanks in advance for any options you can offer!
The Hummer line is now carried by Anatech Ltd. Their address is 6621-F Electronic Drive, Springfield, VA 22151-4303. Phone # is (800) PLASMA-9, their home page is at http://www.anatechltd.com.
You wrote:
} We have an old Hummer coater that was marketed by the Technics Company. } I would like to find out if this company is still in business and, if so, } where they are now located. } Also, who is presently handling the Hummer coater line or who has been } in the recent past. I know there were a number of other models produced } since ours was purchased in the early 80's.
Leslie Eibest SEM lab manager Dept. of Biology Duke University
Does anyone know of a service company in the Pacific Northwest area who provides maintenance for AMRAY SEMs? I would be grateful for the names and email/phone/snailmail addresses for them. Thank you.
Franklin Bailey Electron Microscopy Center University of Idaho Moscow, ID 83844-2204 jfb-at-uidaho.edu
Keep care at the size (in mm) of the CCD device. A collegue has bought a Fuji ??? (I don't remember the reference, it changes so fast !) with F-mount for Nikon optics two years ago, and the CCD is something like 18x25mm. All the optics are shifted in field angle. He uses now a 35mm as "normal" focal and so on. It's not a problem in practice, but if you have a "good" set of optics for 24x36mm, it is not automaticaly that you want with an other device size, with some difficulty to find a wide field which fits (typically a 15 mm).
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Does anyone out there know where one might get a replacement bulb for the Leafscan-45 film scanner? Thanks.
Chris A. Edwards, Mgr. Microscopy and Image-analysis Laboratory Dept. Cell & Developmental Biology The University of Michigan, School of Medicine 4747 Medical Sciences II Bldg. Ann Arbor, Michigan 48109-0616 Office: 734-936-4912 Lab: 734-763-1170 FAX: 734-763-1166
I have now 3 WILD dissecting (stereo compound ;) ) microscope illuminators with cords that do not work. The problem is in the cord from the bulb to the power supply. This is a 6VAC System.
The two prong plug seems to be unique relative to all the other plugs around the Department and here in my lab.
Question: Does anyone know of a supplier for these cords with the two round prongs? This past summer when the last one died I attempted to contact suppliers and came up empty handed but the situation wasn't critical so I moved on. Now it has become critical and this is about the last line of hope I have to get these working again.
The two prongs (both round) are 4mm in diameter and the outside to outside distance is 20mm (inside distance:12mm, c-c:16mm)
Thank you in advance Geoff Williams Microscopy Facility Supervisor
Checkout the new Biology Department Microscopy Facility web page. Version 1 is now On-Line: www.cst.cmich.edu/users/willi1gl/BDMF/BDMF.htm
Angie - You might try Nile Red to "stain" the oil phase in an emulsion. I have used Nile Red in lubrication studies, dissolving the dye (25ppm will do) in the oil phase before making up the emulsion. Omega recommends their X33 filter - "Wide-band TRITC set". As I remember the lit, you needn't be concerned about solubility of Nile Red in any aqueous phase because the dye will only fluoresce significantly in a hydrophobic environment.
Dave Calvert Voridian Division Eastman Chemical Co. P.O. Box 1972 Kingsport, Tennessee Voice: 423-229-4943 Fax: 423-224-7550
Hello, Does anyone know of a fluorescent dye that would specifically tag/stain silicone oil droplets in complex mixtures or suspensions? Thanks in advance for any options you can offer!
Dear listers, I have a friend who has about 10 customers looking for a digital image capture system for their SEMs. I know several vendors are frequenters of this list, but I'm not at all sure that it's inclusive. Does anyone have a really complete list of digital image capture vendors, perhaps even broken down by active vs. passive systems?
Thanks, Ken Converse owner Quality Images third party SEM service Delta, PA
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The next European Microscopy Congress, EMC 2004 (sequel of the EUREM series, last meeting in Brno, 2000), will be held in Antwerp, Belgium, from August 22 till 27, 2004. All members of European societies will recieve a hardcopy of the first circular.
The website www.emc2004.be contains all available information and will be regularly updated. If you are interested to recieve a hardcopy of the
second circular, due September 2003 and containing all information on registration, abstract submission, accommodation, etc. you can fill in the on-line Expression of Interest form for Scientific Delegates.
Commercial Exhibitors are invited to fill in the Expression of Interest form for Exhibitors and Sponsors, after which you will recieve additional information as of April 2003.
We look forward to see you in Antwerp,
Nick Schryvers President EMC 2004
--
Visit http://www.emc2004.be , the official site of the next European Microscopy Congress in 2004
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
My lab has inherited an ElectroScan 2020 ESEM. Does anyone know of any service companies in the Southern US that provide maintenance service for ElectroScan SEMs. If so, please email me their contact information. Thank you. Ty
-- _____________________________________________________ Tyrone L. Daulton Director: Marine Geosciences - Electron Microscopy Facility Physicist and Senior Electron Microscopist
Naval Research Laboratory Marine Geosciences Division (Code 7400) Stennis Space Center, MS 39529-5004
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id PAA17413 for dist-Microscopy; Thu, 12 Dec 2002 15:31:07 -0600 (CST) Received: from njz_spm_filter (sparc5 [206.69.208.10]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with SMTP id PAA17405 for "MicroscopyFilteredEmail3-at-msa.microscopy.com"; Thu, 12 Dec 2002 15:30:36 -0600 (CST) Received: from rosalind.cc.emory.edu (rosalind.cc.emory.edu [170.140.204.4]) by sparc5.microscopy.com (8.9.3+Sun/8.9.3) with ESMTP id PAA17398 for {microscopy-at-sparc5.microscopy.com} ; Thu, 12 Dec 2002 15:30:24 -0600 (CST) Received: from emory.edu (localhost [127.0.0.1]) by rosalind.cc.emory.edu (8.10.2/8.10.2) with ESMTP id gBCLPEc27219 for {microscopy-at-sparc5.microscopy.com} ; Thu, 12 Dec 2002 16:25:14 -0500 (EST) Message-ID: {3DF8FEDD.79FF5B22-at-emory.edu}
Dear List , I have a question. How does on go about punching out a disc of cultured cells on a plastic culture dish for the purpose of a TEM study? Without damaging the cells, of course. If possible please give me some references so I can read about it. Please reply to my e-mail address, below. I have been surfing web references but have as yet not found any for this specific technique.
We have an EMSCOPE SC500 sputter coater which needs repair. As far as I know EMSCOPE is out of business and I am searching for possibilities to repair the unit. Any leads or suggestions?
Thank you,
Krassimir. ______________________________ Dr. Krassimir Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
A user wants to stick down granulocytes. Any suggestions on ways to do this? The user sticks down monocytes with polylysine no problem. Any reason why this polylysine procedure is not working for her granulocytes?
Steve -- Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619) 594-4523 fax: (619) 594-5676 email: sbarlow-at-sunstroke.sdsu.edu http://www.sci.sdsu.edu/emfacility
Chairman, Educational Outreach subcommittee promoting microscopy instruction and increased access to microscopes Microscopy Society of America http://www.msa.microscopy.com/
I think Emscope staff went to Polaron and Emitech. I have the Emitech UK address if you cannot find a local firm.
Dave
On Thu, 12 Dec 2002 16:06:21 -0800 "K.N. Bozhilov" {bozhilov-at-citrus.ucr.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We have an EMSCOPE SC500 sputter coater which needs repair. As far as I know } EMSCOPE is out of business and I am searching for possibilities to repair } the unit. Any leads or suggestions? } } Thank you, } } Krassimir. } ______________________________ } Dr. Krassimir Bozhilov } Central Facility for Advanced Microscopy and Microanalysis } University of California } Riverside, CA 92521 } } Tel.: 909 787 2998 } Fax: 909 787 4324 } ______________________________ } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
FEI (who purchased Philips Electron Optics, who purchased Electroscan) can provide service. The head of SEM service is Scott Shawmeker (sshawmeker-at-feico.com, I think), but he may be out of the country. If you contact the FEI call center at (800) 432-1734, they can direct you to someone who can help you.
You wrote:
} My lab has inherited an ElectroScan 2020 ESEM. Does anyone know of any } service companies in the Southern US that provide maintenance service } for ElectroScan SEMs. If so, please email me their contact } information. Thank you.
Leslie Eibest SEM lab manager Dept. of Biology Duke University
We (Quorum Technologies) continue to support the original Emscope SC500. For local support and service please contact Energy Beam Science (EBS) our distributors in the US:
Energy Beam Sciences 11 Bowles Road PO Box 468 Agawam MA 01001 Tel: 413 786-9322 Fax: 413 789-2786 sales-at-ebsciences.com http://www.ebsciences.com
If you need an operating manual this can be downloaded from the Quorum Technologies website (www.quorumtech.com)
Just a little history. The original UK based Emscope company was sold to Bio-rad Microscience (then the manufacturers of the Polaron range) in 1988. The Polaron / Emscope range was then acquired by VG Microtech (now called Thermo VG Scientific) in 1991, and finally, Quorum Technologies was founded in 2001 following the purchase of the Polaron range from Thermo VG.
Best regards,
Mike Wombwell
**************************************************************************** ********************************************** Quorum Technologies Unit 15A, Euro Business Park New Road, Newhaven East Sussex, BN9 0DQ, UK Tel: +44(0)1273 510535 (main switch board) Tel: +44(0)1273 510620 (direct line) Mobile +44(0)7989 426 431 Fax: +44(0)1273 510536 mike.wombwell-at-quorumtech.com http://www.quorumtech.com http://www.sputtercoater.com http://www.criticalpointdryer.com E & O E
-----Original Message----- } From: K.N. Bozhilov [mailto:bozhilov-at-citrus.ucr.edu] Sent: 13 December 2002 00:06 To: microscopy-at-sparc5.microscopy.com
We have an EMSCOPE SC500 sputter coater which needs repair. As far as I know EMSCOPE is out of business and I am searching for possibilities to repair the unit. Any leads or suggestions?
Thank you,
Krassimir. ______________________________ Dr. Krassimir Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
We recently had a similar problem with oocytes. We finally salvaged some of them by using extended times for adhering them to polylysine cover slips, but our initial try with one hour adhesion times didn't work well.
Although we haven't yet tried it for this purpose, there is an adhesive called Mikrostik which we intend to try in the future. It is available from Ted Pella (Cat. no. 16033) and works well for adhering particulates without engulfing them in glue.
Good luck.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---The Fun Core!! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
-----Original Message----- } From: Steve Barlow [mailto:sbarlow-at-sciences.sdsu.edu] Sent: Thursday, December 12, 2002 6:49 PM To: microscopy-at-sparc5.microscopy.com
A user wants to stick down granulocytes. Any suggestions on ways to do this? The user sticks down monocytes with polylysine no problem. Any reason why this polylysine procedure is not working for her granulocytes?
Steve -- Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619) 594-4523 fax: (619) 594-5676 email: sbarlow-at-sunstroke.sdsu.edu http://www.sci.sdsu.edu/emfacility
Chairman, Educational Outreach subcommittee promoting microscopy instruction and increased access to microscopes Microscopy Society of America http://www.msa.microscopy.com/
I can't answer the question about why it works for one type of cell but not the other - polylysine works on charge which should be the same for them.
Are the cells in the same sample? Have they been treated in the same way? What are the conditions of temperature, fixation, buffer etc.
What is the sample?
Has she tried superfrost plus?
More questions than answers!
Have a good weekend!
Nadolig Llawen a Blwddyn Newydd Dda / God Jul och Gott Nytt År / Merry Christmas and a Happy New Year Obs/NB New postal/visiting address from July 2002!
Gareth Morgan MPhil MSc FIBMS, Institute for Microbiology, Pathology and Immunology (IMPI), H5, Karolinska Institutet, Huddinge Universitetssjukhus, F46 SE 141 86 Stockholm Sweden
OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet för klinisk patologi och cytologi.
NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10. Clinical Histo- and Cytopathology Laboratory.
Hi Jeanette, I have processed cell cultures in dishes with great success with the following protocol: Wash culture with serum-free medium at 37 deg. C Remove medium & fix cells with your primary fix, also at 37 deg. (I use 2.5% glut + 4% pfa + 0.002% picric acid in 0.1M sodium cacodylate, but you should use whatever you wish). The cells will fix almost instantly, but I usually leave them for about 30 min. wash with buffer of choice. Post-fix with osmium 30-60 min. Wash with buffer. Dehydrate using a graded ethanol series. DO NOT USE Propylene oxide...it eats the dishes! Here's where things get slightly different: I embed using a pretty standard "Epon" with the exception that I use LX112 and DMP-30 from Ladd, and DDSA and NMA from EMS. LX112 9.7 gm DDSA 3.2 gm NMA 5.9 gm DMP-30 17 microliter/ml
To embed: Cut the tips off of BEEM capsules to make tubes. Cover the bottom of the dish with a thin layer (about 1-2 mm thick) of the resin and stand the tubes in the resin, over areas of interest if there are any. Polymerize overnight. In the morning top off JUST the BEEM tubes and return to the oven for another day. When you take the dish out, you will be able to grab the BEEM tubes with a pair of needle-nosed pliers and snap them out. Your cells will be on the block face. I usually also get a small amount of the dish, but that separates easily when I trim the blocks. Of course, this will give you en face sections of the cells. If you need vertical sections, cut a wafer off the front of the block & reembed it.
Call if you have any questions. good luck, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Jeannette, We routinely process cultured cells for TEM per the following protocol:
Falcon and Corning dishes seem to work better than CoStar if you have a choice. Fix, wash, osmicate, wash and dehydrate with ethanol as usual but with shorter times since you have a monolayer. Do not use propylene oxide as it will dissolve the dish. After the 100% ethanol step, pour all the alcohol off the sample and put resin on. Use an epoxy type resin such as LX 112, eponate 12 or epon 812, but not Spurr's. Do NOT use a 50/50 mixture of alcohol and resin. (This combination appears to partially dissolve the dish while the components used separately do not.) Change the resin 2 times over a period of 4 hours. Pour off the resin. Fill beam capsules with resin and invert them on the sample. If the dish is small a hemostats are useful for inverting the capsules. Polymerize for 36 hrs at 60 degrees. Let cool and pop blocks off the dish. The cells will come off the dish cleanly with no damage.
Call or email me if you have questions and good luck. Mary Gail Engle
PourAt 04:25 PM 12/12/02 -0500, Jeannette Taylor wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Mary Gail Engle Sr. Research Laboratory Manager Electron Microscopy & Imaging Facility Health Sciences Research Bldg. 001 University of Kentucky Lexington, KY 40536-0305
David Patton wrote: =========================================== I think Emscope staff went to Polaron and Emitech. I have the Emitech UK address if you cannot find a local firm. ========================================= At one time there was a free-standing company (founded by Dr. Gerald Kaye) in Watford, England called Polaron Ltd. In around 1982 the company was acquired by BioRad Laboratories, Inc. and it became part of the BioRad Microscience Division. At about that time, either before or after the acquisition (my memory now starts to fade) but under Dr. Kaye's tenure, the firm acquired Emscope. So the entire Emscope operation was absorbed into what the marketplace then knew as Polaron.
With the passing of years, the Polaron part (or what became known as the Polaron EM Prep Lab business) was sold off (by BioRad) to Fisons Instruments (VG MIcrotech Division), then that whole part of Fisons was acquired by Thermo Instruments and about two years ago, what was left of the original Polaron, was spun out and acquired by the senior management of the unit and is now called
Quorum Technologies Unit 15A Euro Business Park New Road Newhaven East Sussex BN9 0DQ England Tel (+44) 01273 510535 Fax (+44) 01273 510536
My main contact there is Mr. Mike Wombwell (mike.wombwell-at-quorumtech.com).
They continue to manufacture the Polaron line of equipment and much to my great pleasure, they are maintaining the tradition of supplying parts for old instruments. We had a need for a replacement transformer for a vacuum evaporator that was manufactured in about 1985 but getting it was no problem . Some of the current Quorum products have progenies that I think can be traced back to some of the old Emscope products.
So if you need a replacement part for an old Emscope unit, contact Quorum Technologies.
Disclaimer: SPI Supplies has no vested interest in Quorum, however we do purchase certain items from time to time from them (and they from us). They are our competitor and a highly reputable one at that!
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
Jeanette, Do you want to remove the cells before or after fixation & embedding? If before, have them put some thermolux plastic cover slips into the culture dishes and then you can easily remove one to fix it without damaging the rest of the culture.
If you want to fix and embed in the culture dish then you can do one of a number of things:
A) X-section of culture: Add enough embedding resin (must infiltrate with resin + ETOH not Propylene oxide or acetone which will dissolve the plastic...this will work with EPON generics) to make a thickness equivalent to that of a flat embedded section. Later break away the petri dish and cut out pieces of the embedded sample with a jewelers saw for microtomy.
B) Horiz. section: Polymerize with a slightly thinner amount of resin so that you can still break the dish away, easily cut small pieces of the culture and attach them to specimen blocks for microtoming.
C) Horiz section: Polymerize cells with a very thin coat of resin. While this is polymerizing place beem or gelatin capsules, that have had their ends cut off, over the cells of interest so that the capsules will be secured to the dish during polymerization. Fill the capsules up with resin and again polymerize. Break the specimen capsule away from the dish bringing with it part of the culture.
If you need to check the cells prior to selecting ones to cut, you can polymerize the culture with the very thin resin layer and then examine it under a microscope to select your cell areas. Then place the capsules over those areas, add a drop of resin and let polymerize. Once capsules are sealed to the surface you can fill them up and again polymerize.
This is a very easy method to use to make multiple blocks without need to do lots of sawing and cutting hard resins. You can do similar things with cells grown on coverslips.
If orientation isn't important, just fix cells in culture dishes, scrap off and pellet using a little agarose to hold the pellet together if necessary so it can be cut into small "blocks", dehydrate, infiltrate, embed and you are all set.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building West Lafayette, IN 47907
On 12/12/02 4:25 PM, "Jeannette Taylor" {jvtaylo-at-emory.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear List , I have a question. How does on go about punching out a disc } of cultured cells on a plastic culture dish for the purpose of a TEM } study? } Without damaging the cells, of course. If possible please give me some } references so I can read about it. Please reply to my e-mail address, } below. } I have been surfing web references but have as yet not found any for } this specific technique. } } thanks, Jeannette Taylor } jvtaylo-at-emory.edu } } }
I wouldn't punch our a disc from plastic culture dishes. Different plastic dishes can partially dissolve depending on the embedding media used.
Tell the researcher that the best way to look at cultured cells for TEM is to use ACLAR Embedding Film from EM Sciences. It can be cut to size to fit in a petri dish and the cells easily attach. Then you can process the Aclar/cells as if normal its a normal tissue sample, embedding it the film with the cells face down in a mold. It's a rare cell type that won't grow on these sheets, so there should be no problem to plate cells onto the ACLAR.
You can buy the ACLAR embedding film from Electron Microscopy Sciences, look on page 140 in their catalog.
A second method would be to have the researchers grow cells on thick glass coverslips and "pop-off" the cells after embedding and polymerization of the epoxy resin. If you want that technique email me directly.
Good Luck!
Karen Bentley
Karen L. Bentley, M.S.(previously Jensen) Associate Scientist & Project Manager Electron Microscope Research Core University of Rochester Medical Center Rochester, NY 14642 585-275-1954
-----Original Message----- } From: Jeannette Taylor [mailto:jvtaylo-at-emory.edu] Sent: Thursday, December 12, 2002 4:26 PM To: Microscopy
Dear List , I have a question. How does on go about punching out a disc of cultured cells on a plastic culture dish for the purpose of a TEM study? Without damaging the cells, of course. If possible please give me some references so I can read about it. Please reply to my e-mail address, below. I have been surfing web references but have as yet not found any for this specific technique.
Do you mean just taking a few and leaving the rest on the plate (why would you want to "punch out" the cells?)?
There are several ways to embed adherent cells in tissue culture plates. Tell us more about what you want to do, and we'll try to help.
Also, see Miller SE. 1985. Electron microscopy of tissue culture. In Jones BR, Electron Microscopy: 41 Exercises by 17 Scientists. Library Research Associates Inc, Monroe NY. pp 293-315.
Sara Miller
On Thu, 12 Dec 2002, Jeannette Taylor wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear List , I have a question. How does on go about punching out a disc } of cultured cells on a plastic culture dish for the purpose of a TEM } study? } Without damaging the cells, of course. If possible please give me some } references so I can read about it. Please reply to my e-mail address, } below. } I have been surfing web references but have as yet not found any for } this specific technique. } } thanks, Jeannette Taylor } jvtaylo-at-emory.edu } } } }
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
The carbon black industry has developed a particle sizing procedure through ASTM International (D24 Committee on Carbon Black). The D24 group has recently rewritten and updated the standard, D3849. The procedure is copyrighted so no one can/should just give you a copy. However, that procedure may be of use to you and can be purchased...about $30 on-line I think. Disclaimer: I am a member of ASTM International and represent my company's interests at those meetings. I don't receive compensation but peripherally benefit from the sale of D24 standards because that money does support some research activities of the D24 group.
What I can tell you is that most of us doing particle sizing (down to 10 nm particles) buy our carbon coated grids from one of several EM supply companies. That should solve most background problems. Further, if particle size is what you want, drop your accelerating voltage to increase contrast so that the particle is as close to black as possible. Then you can use any number of image analysis programs to threshold out background gray junk and then identify (e.g. circle in red) all the particulate. We also deselect any particulate we know is not of interest. Your choice of magnification is critical. Too low and your particle will have too few pixels to be relevant. Too high a magnification and you will be taking too many images and greatly extending analysis time. Dispersion of your particulate on the grid will also greatly affect the speed of analysis. Too disperse will cause you again to take lots of images and too concentrated will cause overlaps.
You have already been given some good advice by others. Hope this helps too.
Chuck Butterick Degussa Engineered Carbons
Ian MacLaren {maclaren-at-tu-dar To: Microscopy {Microscopy-at-sparc5.microscopy.com} mstadt.de} cc: Subject: TEM Particle size measurement 12/10/2002 10:22 AM Please respond to ian.maclaren
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Dear all, Are other people doing particle size measurement from TEM images, and if so how? The particles are small metal catalyst particles on carbon supports. One problem is that automatic image processing could fail because of the background contrast ripples from the carbon. At the moment the student is simply measuring everything with a ruler, and this takes ages, of course.
Secondly, does anyone have a good reference on particle size distribution? Specifically, we would like to know how distributions are best represented statistically, particularly when they are non-normal (short of just showing a diagram for each one).
Best wishes -- Ian MacLaren Strukturforschung, Materialwissenschaft, TU-Darmstadt, Germany ian.maclaren-at-physics.org / http://homepages.tu-darmstadt.de/~maclaren/
Dear Listers, thank you all very much for the very helpful suggestions and leads. I have gotten a request to forward your responses to another, Silvia Pasquetto, who is also interested in this subject. Does anyone object?
michael shaffer wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Jim Haley writes ... } } } ... } } The '30 day warranty' [...] is certainly enough time } } to test the adapter out and verify its quality and } } determine if the adapter is satisfactory. ... } } Before we order, or request a trial, how do we best determine what type of } 'C-mount' is required. When I looked into this more than a year ago, a 1X } would have been best for a 9xx Coolpix and its CCD. Is there a formula for } determining the mag factor of the C-mount adapter relative to the size of } the CCD in the camera???
If you are going the C-mount route, 1X is always the best to use in conjunction with our adapters at this point, regardless of camera model. Bear in mind that in most cases, we can mount to the trinocular port which eliminates any C-mount concerns and is less expensive. I usually only recommend going through a C-mount if you have a number of scopes from different manufacturers and already have 1X C-mount adapters for them.
} Also ... is the adapter different with regard to older Nikon 9xx and newer } 5xxx cameras??
The 950, 990, 995 and 4500 require no special adapter rings. The 5000 does require an adapter ring, but is a straightforward and works very well. The 5700 requires both an extender and a thread adapter. I am recommending at this point that users stay away from this model as it is prone to EXTREME vignetting with most scopes, and the digital zoom mode of the camera MUST be used to eliminate the vignetting, which can degrade image quality.
We do carry all the adapters and spacers mentioned above.
In fact the adapter for the 5000 is being discounted by $20 until December 31st.
Just in case it isn't obvious: COMMERCIAL DISCLAIMER: MVIA, Inc. is the supplier of the items discussed above
Thanks & Happy Holidays! Jim Haley
****************************** Jim Haley Applications Engineer MVIA, Inc. 125 Sherwood Drive Monaca, PA 15061 voice: (724) 728-7493 fax: (412) 291-1709 e-mail: haley-at-mvia.com webpage: http://www.mvia.com ******************************
Another approach is to do your cultures in 96-well plates (flat bottom wells). In this case the wells become acceptable "BEEM capsules," with the cells in favorable position for sectioning at the tip of the capsule. For further details of this approach, see Christensen AK and Bourne CM, 1999, "Shape of large bound polysomes in cultured fibroblasts and thyroid epithelial cells," Anatomical Record 255:116-129.
--Kent
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A. Kent Christensen, Professor Emeritus Department of Cell and Developmental Biology, Medical Science II Building University of Michigan Medical School, Ann Arbor, MI 48109-0616 Tel (work) (734) 763-1287, Fax (work) (734) 763-1166 akc-at-umich.edu http://www.umich.edu/~akc/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
--On Thursday, December 12, 2002 4:25 PM -0500 Jeannette Taylor {jvtaylo-at-emory.edu} wrote: } } Dear List , I have a question. How does on go about punching out a disc } of cultured cells on a plastic culture dish for the purpose of a TEM } study? } Without damaging the cells, of course. If possible please give me some } references so I can read about it. Please reply to my e-mail address, } below. } I have been surfing web references but have as yet not found any for } this specific technique. } } thanks, Jeannette Taylor } jvtaylo-at-emory.edu }
Hello, Does anyone have any 3-color or 4-color transfers for the Phaser IIsdx dye sublimation printer that they would like to sell? Xerox no longer has any stocks of color transfer rolls.
I've still a lot of paper to use up and some users who appreciate the reliability of that printer.
Please no flames about the 'superiority' of ink jets. We recognize the relative merits of all printer types. This Phaser has been reliable and reproducible, we intend to run it into the ground as long as supplies are available.
Thanks in advance, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires Windows 95 or better", so I bought a Macintosh. ************************************************************************ ******
Happy holidays to everyone, good luck in the new year.
Someone just asked me if I knew anything about getting microradiographs done. I didn't so, if you do, could you help me out?
The project requires about 200 microradiographs of thin sections from human femur with a resolution level that will show osteons. They didn't know exactly what resolution, they guessed in the micrometer range.
We are in N. Calif. so if you know someone who can do this let me know so I can pass on the contact. They are working on a budget proposal and would like to get an idea of time and cost.
Thanks
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
Hi We use a technique for punching out discs of cultured cells for introduction to the high pressure freezer. This technique works just as well for conventional fixation.
Grow the cells on membrane inserts in well plates. Use a disposable biopsy punch to cut out the discs. We get our plates from Fisher Scientific: 3 micron, clear, transwell inserts in 6 or 24 well plates. I have one catalogue number of CS003472 The biopsy punches we get from Dormer Labs - 1.5 mm Acu Punch. They just fit a high pressure freezer hat or give a good size for conventional fixation.
The membranes get processed along with the sample and cut with a diamond knife just fine.
We have also used Aclar film and it works fine too for conventional fixation. The aclar peels off after polymerizing the resin, leaving the the cells in the resin, or it can be cut with a diamond. Aclar is really too thick for high pressure freezer hats, and the transwell membranes are thin enough. Elaine
} Jeanette: } } I wouldn't punch our a disc from plastic culture dishes. Different plastic } dishes can partially dissolve depending on the embedding media used. } } Tell the researcher that the best way to look at cultured cells for TEM is } to use ACLAR Embedding Film from EM Sciences. It can be cut to size to fit } in a petri dish and the cells easily attach. Then you can process the } Aclar/cells as if normal its a normal tissue sample, embedding it the film } with the cells face down in a mold. It's a rare cell type that won't grow } on these sheets, so there should be no problem to plate cells onto the } ACLAR. } } You can buy the ACLAR embedding film from Electron Microscopy Sciences, look } on page 140 in their catalog. } } A second method would be to have the researchers grow cells on thick glass } coverslips and "pop-off" the cells after embedding and polymerization of the } epoxy resin. If you want that technique email me directly. } } Good Luck! } } Karen Bentley } } Karen L. Bentley, M.S.(previously Jensen) } Associate Scientist & Project Manager } Electron Microscope Research Core } University of Rochester Medical Center } Rochester, NY 14642 } 585-275-1954 } } } -----Original Message----- } } From: Jeannette Taylor [mailto:jvtaylo-at-emory.edu] } Sent: Thursday, December 12, 2002 4:26 PM } To: Microscopy } Subject: technique } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-- Dr. Elaine Humphrey Director, BioImaging Facility First Vice President, Microscopy Society of Canada University of British Columbia 6270 University Blvd, mail-stop Botany Vancouver, BC CANADA, V6T 1Z4 Phone: 604-822-3354 FAX: 604-822-6089 e-mail: ech-at-interchange.ubc.ca website: www.emlab.ubc.ca
I used to process cells in culture dishes for EM. I processed the whole dish by adding each reagent to the dish, and then after polymerisation I cut out the required bit with a hacksaw. The dish does start to dissolve, but if you go straight from anhydrous EtOH to 50/50 EtOH/Epon, without propylene oxide or any other intermediary, it doesn't cause too much of a problem. Because you are dealing with a thin layer of cells, you don't need to leave each reagent in the dish for very long in the resin stages, and that helps too.
Lesley Weston.
on 13/12/2002 7:05 AM, Jensen, Karen at Karen_Jensen-at-urmc.rochester.edu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Jeanette: } } I wouldn't punch our a disc from plastic culture dishes. Different plastic } dishes can partially dissolve depending on the embedding media used. } } Tell the researcher that the best way to look at cultured cells for TEM is } to use ACLAR Embedding Film from EM Sciences. It can be cut to size to fit } in a petri dish and the cells easily attach. Then you can process the } Aclar/cells as if normal its a normal tissue sample, embedding it the film } with the cells face down in a mold. It's a rare cell type that won't grow } on these sheets, so there should be no problem to plate cells onto the } ACLAR. } } You can buy the ACLAR embedding film from Electron Microscopy Sciences, look } on page 140 in their catalog. } } A second method would be to have the researchers grow cells on thick glass } coverslips and "pop-off" the cells after embedding and polymerization of the } epoxy resin. If you want that technique email me directly. } } Good Luck! } } Karen Bentley } } Karen L. Bentley, M.S.(previously Jensen) } Associate Scientist & Project Manager } Electron Microscope Research Core } University of Rochester Medical Center } Rochester, NY 14642 } 585-275-1954 } } } -----Original Message----- } } From: Jeannette Taylor [mailto:jvtaylo-at-emory.edu] } Sent: Thursday, December 12, 2002 4:26 PM } To: Microscopy } Subject: technique } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear List , I have a question. How does on go about punching out a disc } of cultured cells on a plastic culture dish for the purpose of a TEM } study? } Without damaging the cells, of course. If possible please give me some } references so I can read about it. Please reply to my e-mail address, } below. } I have been surfing web references but have as yet not found any for } this specific technique. } } thanks, Jeannette Taylor } jvtaylo-at-emory.edu } }
Dear group- someone brought me an unidentified artifact supposedly found in some pyramid that dates to classic Maya, however the style of carving and the kind of stone used they say is not Mayan. Outside of conducting an EDS analysis with our SEM - do you have any other suggestions? The elements that came up was Al, Si, Na, C , K and minimal Mg - does this suggest any particular type of stone to you? Thanks Barb
This may be the wrong list for this question. I suggest Ask-A-Mineralogist at {http://www.minsocam.org} the other and older MSA, the Mineralogical Society of America.
When dealing with a "stone" - presumably so fine-grained that you can't see any crystal shapes in a binocular microscope - the important characteristics will be color, texture, specific gravity and hardness. Let's assume it is fuchsite. Fuchsite is a green mica and as such is softer than a pen knife. Jadeite on the other hand, also a Na, Al silicate is harder than a pen knife. A simple scratch test will determine the relative hardness.
What color is it?
On Sunday, December 15, 2002, at 08:45 AM, barbara maloney wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear group- someone brought me an unidentified artifact supposedly found } in some pyramid that dates to classic Maya, however the style of carving } and the kind of stone used they say is not Mayan. Outside of conducting } an EDS analysis with our SEM - do you have any other suggestions? The } elements that came up was Al, Si, Na, C , K and minimal Mg - does this } suggest any particular type of stone to you? } Thanks } Barb } } }
Dr. Gordon Nord Senior Scientist Environmental Sciences Laboratory Brooklyn College Brooklyn NY 11210
-- Hi, I have been reading, with great interest, the replies to Jeannette Taylor's problem with embedding her cells. I encounter a similar problem when I try and flat embed cultured cells for cryo-ultramicrotomy. I have tried to grow Hela cells on both glass and plastic and then embed in 12% gelatin and infiltrate in 2.3M sucrose. The only time the cells have come away from the substrate, successfully, was when one of the samples was fixed heavily for morphological studies. If the samples are fixed lightly ie 2% para-formaldehyde, the cells remain firmly adhered to the substrate even after 2 weeks infiltration in sucrose! Has anyone had similar problems? Is there a simple answer to this? Answers on a Christmas card...
Ken Blight Senior Scientific Officer Cancer Research UK London England
Barbara - If you have access to a geologist, he/she may be able to identify the type of stone for you, and if there are geological maps of the region from which the artifact came, he/she may be able to identify its provenance. Probably over half the rocks on the planet contain the elements you list, so that won't be much help. Not that many types of rock lend themselves to manual shaping to make things out of them, though, so that could narrow it down. Soapstone is a famous example from the Canadian Arctic, but there are lots of others. Even the various minerals that make up rocks can be tricky to ID with EDS, since so many of them share typical element compositions. XRD of a bit of ground-up artifact may identify constituent minerals, but lots of archaeology types hate having people grind up their stuff, so that may not be an option. An interesting problem, though.
F.C. Thomas FThomas-at-NRCan.gc.ca, (902) 426-4635, facsimile/telecopier (902) 426-6152 GSC Atlantic Natural Resources Canada/Ressources naturelles Canada Bedford Institute of Oceanography/ l'Institut Oceanographique du Bedford P.O. Box 1006/B.P. 1006, Dartmouth, Nova Scotia/Nouvelle Ecosse B2Y 4A2 Government of Canada/Gouvernement du Canada ----- Original Message ----- } From: "barbara maloney" {maloneyb-at-fiu.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Sunday, December 15, 2002 9:45 AM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bbauer-at-icc.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, December 16, 2002 at 10:24:38 ---------------------------------------------------------------------------
Email: bbauer-at-icc.edu Name: Bambi Bauer
Organization: Illinois Central College
Education: Undergraduate College
Location: East Peoria, Illinois, USA
Question: Our college is looking to purchase a comparative, polarized microscope for our new Criminal Justice Program. Are there alternatives to the Leica Microsystems, Inc - CFM2 microscope, with the same quality and features? What does your organization recommend as possible alternatives? What do you use?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rco2-at-ufl.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, December 16, 2002 at 14:22:18 ---------------------------------------------------------------------------
Email: rco2-at-ufl.edu Name: Robin Oliver
Organization: University of Florida
Education: Graduate College
Location: Gainesville, FL, USA
Question: I am currently trying to look at two GFP transformed bacterial strains in the xylem of plant tissue with a confocal microscope. I started out making think hand sections but the lamp (i think) was so hot, it started pulling water out of the sample. This not only made bubbles under my sample but also made it impossible to increase the magnification.
Then, I tried to get rid of the water in my samples by drying them out overnight. This made the autofluorescence (yellow) of the tissue so intense, it was virtually impossible to see my two bacterial strains (yellow and blue).
So, how can I either get rid of the water in my sample or quench the autofluorescence of my plant tissue. There is some debate at this point as to if using a freezing microtome to cut my samples would decrease the water as well as the autofluorescence. Finally, should I use a special mounting medium, would this help these problems?
Any help anyone could give would be greatly appreciated!!!
Hi All, I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to put a LN2 cold trap on it, in an attempt to clean up the vacuum a tad more. There is a 3.5 inch by 4.5 inch plate right above the diffusion stack which looks to be a great place to attach a cold trap. Rumor has it that this was an option. Are there any old traps kicking around that one can acquire? I could have our machine shop manufacture one, but was hoping for a cheaper route. Thanks in advance, Randy Nessler 319-335-8142
Dear List, Does anyone know where to obtain tobacco mosaic virus to use as an internal calibration standard? TIA for your help. Yours, Bill Tivol EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
G'day Guys, Some years ago Zeiss produced a kit for demonstrating diffraction and aberrations in the optical microscope. I recently obtained one of these kits, it seems almost complete, but I require a copy of the instructions for its use. If I remember correctly it was a small booklet in German. If anyone has a copy or even better an english translation or set of instructions would it be possible to get a copy. Thanks Merry Christmas and Happy New Year to all
Regards
John V Nailon Executive Officer and Operations Manager The Centre for Microscopy and Microanlaysis The University of Queensland St Lucia QLD 4072 Tel: +61-7-33654214 Fax: +61-7-33654422 WWW: http://www.uq.edu.au/nanoworld
I have come across a Gatan model 628/2 single tilt hot stage (1300C) and heating control unit that was purchased for a Hitachi H600 100Kv TEM but was never used. It was apparently tucked away in the lab by my predecessor immediately after its purchase and was recently discovered after having already replaced this microscope. I would like to know if anyone with a similar microscope is interested in actually using it.
Please contact me off-line to discuss in more detail.
George R. Munzing Jr. Engelhard Corporation 25 Middlesex-Essex Tpk. Iselin, NJ 08830 TELE 732-205-7030 FAX 732-205-5300
} I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to } put a LN2 cold trap on it, in an attempt to clean up the } vacuum a tad more. ...
Just to point out (... in case someone has one of these laying around ..), the trap doesn't necessarily have to be LN type. A cold water chevron trap will also work very effectively.
cheerios ... shAf :o) Avalon Peninsula, Newfoundland www.micro-investigations.com (in progress)
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: bbauer-at-icc.edu [mailto:bbauer-at-icc.edu] Sent: Monday, December 16, 2002 5:10 PM To: Microscopy-at-sparc5.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bbauer-at-icc.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, December 16, 2002 at 10:24:38 ---------------------------------------------------------------------------
Email: bbauer-at-icc.edu Name: Bambi Bauer
Organization: Illinois Central College
Education: Undergraduate College
Location: East Peoria, Illinois, USA
Question: Our college is looking to purchase a comparative, polarized microscope for our new Criminal Justice Program. Are there alternatives to the Leica Microsystems, Inc - CFM2 microscope, with the same quality and features? What does your organization recommend as possible alternatives? What do you use?
On Mon, 16 Dec 2002, by way of Ask-A-Microscopist wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (rco2-at-ufl.edu) from } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, } December 16, 2002 at 14:22:18 } --------------------------------------------------------------------------- } } Email: rco2-at-ufl.edu } Name: Robin Oliver } } Organization: University of Florida } } Education: Graduate College } } Location: Gainesville, FL, USA } } Question: I am currently trying to look at two GFP transformed } bacterial strains in the xylem of plant tissue with a confocal } microscope. I started out making think hand sections but the lamp (i } think) was so hot, it started pulling water out of the sample. This } not only made bubbles under my sample but also made it impossible to } increase the magnification. } WHY DON'T YOU USE A COVERSLIP AND SEAL THE EDGES TO KEEP IN THE WATER?
} Then, I tried to get rid of the water in my samples by drying them } out overnight. This made the autofluorescence (yellow) of the tissue } so intense, it was virtually impossible to see my two bacterial } strains (yellow and blue). } IS YOUR TISSUE FIXED IN GLUTARALDEHYDE? GLUT IS AUTOFLU0RESCENT.
YOU CAN TRY QUENCHING THE AUTOFLUORESCENCE WITH SOMETHING LIKE GLYCINE OR AMMONIUM CHLORIDE. I'M NOT SURE THIS WILL WORK, BUT YOU CAN TRY IT.
} So, how can I either get rid of the water in my sample or quench the } autofluorescence of my plant tissue. There is some debate at this } point as to if using a freezing microtome to cut my samples would } decrease the water as well as the autofluorescence. Finally, should } I use a special mounting medium, would this help these problems? } SOME MOUNTING MEDIA CONTAIN GLYCEROL AND GLYCINE. IF VIEWING THE TISSUE WET WORKS, START WITH JUST KEEPING IT WET BY SEALING IT (E.G., WITH FINGERNAIL POLISH AROUND THE EDGES). THEN, IF THAT DOESN'T DO IT, TRY THE GLYCEROL/GLYCINE.
IF YOU NEED FLATTER SECTIONS, TRY A TISSUE SLICER. SEVERAL EM SUPPLIERS CARRY THEM.
} Any help anyone could give would be greatly appreciated!!! } } R. Oliver } GOOD LUCK, SARA MILLER } --------------------------------------------------------------------------- } } }
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Hello, I received cells that have been sitting in potassium dichromate and the researchers requested that I do propane jet freezing. I don't have any experience with cells that are treated in this manner before processing for TEM. I would appreciate hearing from anyone have comments on the eventual quality of fixation (of any sort) or other concerns. Thanks John Shields EM Lab Univ. of Georgia
I'm a Firearm and Toolmark examiner so do not work with polarized light comparison scopes but do work with comparison scopes in a forensic lab. When I worked at ISP lab Morton I occasionally lectured at the Criminalistics overview course that ICC had at that time, one or two lectures a semester as a guest of the instructor. Here are some leads that might help.
Leeds http://www.leedsmicro.com/ offers basically customized comparison microscopes, their hair and fiber scopes are based upon Olympus Scope bodies, they would gladly use pol. scopes for the extra cost I'm sure. When I talked to them about a firearms scope they indicated they basically custom built each scope for a customer but for their firearms scope they use a zoom lens system and I do not like zoom lens systems. Our lab just bought one of their hair comparison scopes and our hair and fiber people like it quite a bit. They didn't get the pol. scope as they do their pol. light mic. on the single pol. scope then go to the comparison scope for side by side examination.
Leica http://www.leica.com/index.html offers the UFM-4 which is designed for firearms and Toolmark work, they offer a hair and fiber scope (I don't know the model #) as well but neither is a pol. scope as such but they may offer something that I haven't seen. They may well make accessories available that would let you convert their scope. I've added sub-stage polarizing filters for work comparing fingernails in the past to the UFM-4 and as indicated the UFM-4 is not designed for the type work you would be doing with a polarized light comparison scope.
Most forensic labs don't use a polarized comparison scopes except occasionally. It use to be very difficult to get good light balance and you didn't want to add polarizers to the problem. The newer scopes have good light balance that can be more easily dealt with. With the new microscopes and their easily balanced lighting systems many more labs may go that way. You are looking at adding expensive stages and strain free optics so quite a bit more money, a few thousand more, on an already expensive microscope.
You may want to get together with Ill. State Police Lab Morton (309-284-6500) which is very close to you and talk to them about comparison scope use. I don't know if they have anyone on staff there that is doing hair or fiber work right now (staff has changed a great deal in the 13 years I've been gone). But they could put you together by phone with one of the people that teach the work in their training lab if not. I'm sure that the lab staff would still be glad to help if asked. Before you spend around 40 or 50K I'd talk to as many people as you can.
Jim
James L. Roberts Firearm & Toolmark Examiner Ventura Co. Sheriff's Lab 800 S. Victoria Ave. Ventura, CA. 93009
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us
} } } {bbauer-at-icc.edu} 12/16/02 04:09PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bbauer-at-icc.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, December 16, 2002 at 10:24:38 ---------------------------------------------------------------------------
Email: bbauer-at-icc.edu Name: Bambi Bauer
Organization: Illinois Central College
Education: Undergraduate College
Location: East Peoria, Illinois, USA
Question: Our college is looking to purchase a comparative, polarized microscope for our new Criminal Justice Program. Are there alternatives to the Leica Microsystems, Inc - CFM2 microscope, with the same quality and features? What does your organization recommend as possible alternatives? What do you use?
You should be aware that installing a cold trap or baffle between the diffusion pump and vacuum chambew will decrease the pumping speed of the system significantly - usually by about a factor of two. This will, in turn, increase the time required to pump down to an operating vacuum. You need to take this effect into consideration before adding such a device to your evaporator. The pumnpdown process is discussed in some detail in Chapter 2 of my book 'Vacuum Methods in Electron Microscopy' (for a description see: http://www.2spi.com/catalog/books/book48.html) and the characteristics of traps and baffles are discussed in Chapter 5. -- Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan 3062 Dow Bldg.; 2300 Hayward St. Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-662-5237
OK Bill here goes, I wanted to know too. http://www.asu.edu/clas/csss/SPM/IG_gifs/asutmv2.html http://masspec.scripps.edu/publications/pdf/2001_AngewChem.pdf and finally the source of sources, http://www.atcc.org/SearchCatalogs/PlantVirology.cfm (in the event that the next is broken)
I thought I remembered that the ATTC had the stuff.
Regards to you both,
A Merry Xmas and Happy New Year to all,
Fred Monson
Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone: 610-738-0437 eMail: fmonson-at-wcupa.edu An FEI (Quanta 400 and Technai 12), Oxford INCA Energy 400, and Olympus FV-300 Shop.
-----Original Message----- } From: Bill Tivol [mailto:tivol-at-caltech.edu] Sent: Monday, December 16, 2002 7:16 PM To: microscopy-at-sparc5.microscopy.com
Dear List, Does anyone know where to obtain tobacco mosaic virus to use as an internal calibration standard? TIA for your help. Yours, Bill Tivol EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
Hi I am a technician in earth science department in sultan Qaboos University i am working in SEM lab, I have JOEL 840A JSM with link Isis 300, I am looking for training in in this machine but unfortunately I did not find good one for me yet, . No I am trying again hope get chance this time. If any one can help me in this. thanks
saif
Saif Amer Al Mammari Sultant of Oman, Muscat Sultan Qaboos University College of Science Earth Science Department
We don't have the D100, but we do have the Kodak 760. The Kodak 760, along with the Nikon D1 and D1X all based on very similar professional level true SLR bodies with the Nikkor AF / F-mount, D-Type lens mount and autofocusing system. The 760, D1, DH, D1x are based on the professional F5 body, the D100 is based on the F80 body (albeit modified). The D100 is matched against the Canon EOS-D60 in general performance.
As far as mounting any of these cameras on a microscope goes its very easy, if you have a scope with a trinocular head just treat these cameras like a normal 35mm film camera. Use a standard F-mount or F-mount adapter from the microscope vendor. You will need to adjust from the standard photoeye piece lens. Since the (Sony) CCD sensor is 23.7 x 15.6 mm or 1.8" / diagonal 28.4 mm (APS sized) its a little smaller than the standard 35mm format. The APS C-type film format is 23.4 x 16.7mm, and 35mm film format is 35.0 x 23.3 - therefore there is a 1.5x focal multiplier factor difference between the D100 sensor and 35mm film so you will need a lower mag photoeye piece if you want the whole field and not the "sweet spot". However all of these cameras are designed to (1) work with standard film format lenses, and (2) be SLR so you see what you get.
(Side note: "The EOS-1Ds is Canon's newest professional SLR. Based on the EOS-1D body the EOS-1Ds raises resolution to 11 megapixels, uses a CMOS sensor (just like the EOS-D30 and D60) and is the first Canon digital SLR with a sensor which captures a full 35 mm frame. " --- Dec. 17, 2002)
A Draw back for microscopy work is a fixed viewfinder prism and focusing screen. I know with the Kodak 760, the first thing I did was replace the focusing screen (Nikon M and C Focusing Screens work well) and got the Nikon DW-31 High Mag (6x) Finder (Right angle finder so you don't have to climb up on top of the scope to look through the camera view finder.
For a solid photographic review of the camera go and see: http://www.dpreview.com/ (they review digital cameras) They also included a series of full resolution images from each camera reviewed as well as user reviews.
} List: } Does anyone have experience with Nikons new low end professional digital SLR } offering, model D100? It uses a standard Nikon SLR body and mount so most of } their lenses fit and auto features will work with many of the lenses (AF & D } type that we use). It is a 6 mega pixel camera listing for ~$2,000 (body only). } Since we currently use Nikon SLR camera's on our OM systems and have several } Nikon lenses for copy stand work this seems like a very good fit. Considering } all of the costs associated with purchasing and mounting fixed lens consumer } camera's onto microscopes it appears to be a viable option? True the D100 costs } are slightly higher however the benefits are significant, high resolution } images, selectable lens ranges, improved lens quality, versatile camera } software, and I am assuming it uses standard microscope mounting hardware found } in many labs. } } What have I missed? Are there pitfalls associated with this camera? Since this } is a newer camera model I can not confirm some of the microscope mounting } information. Please correct any errors or misinformation above. Any additional } input or insight would be appreciated. } } Sincerely, jr } } } } John Robson } Boehringer Ingelheim Pharmaceuticals, Inc. } PO Box 368 } 900 Ridgebury Rd } Ridgefield, CT 06877 } } Phone (203)798-5640 } Fax (203)798-5698 } } e-mail jrobson-at-RDG.boehringer-ingelheim.com } } } -----Original Message----- } } From: "Mortro-at-aol.com"-at-sparc5.microscopy.com } [mailto:"Mortro-at-aol.com"-at-sparc5.microscopy.com] } Sent: Tuesday, December 10, 2002 11:08 AM } To: haley-at-mvia.com; atcsem-at-earthlink.net } Cc: jfactor-at-purvid.ns.purchase.edu; Microscopy-at-sparc5.microscopy.com; } atcsem-at-earthlink.net } Subject: Re: LM: CCD camera for LM } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I'd recommend MVIA's Coolpix adapters. We have been using them in the lab } for a few months now. } } We had tried unsuccessfully to get good images using our Coolpix 995 camera with } an adapter from a different vendor. This summer we decided to try the adapters } from MVIA. The adapters have worked great for us and we can now get good images } from the camera. }
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 350 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
} listers } } i have an old sorvall MT-1 ultramicrotome that i would like to put back into } service. i am lacking the base stand that had the "boom" for holding the } dissecting microscope as well as the fluorescent light fixture. if any of you } have these in your storage closet, i would be glad to take them off your hands } and free up some storage space for you. of course, i would also pay for } shipping and/or a modest fee for the items. } } please respond off-list if you can help me. } } thanks and happy holidays } } tbudd } -- } Dr. T. Budd } Chair of Biology } St. Lawrence University } Canton, NY 13617 } Phone = 315-229-5640 } Fax = 315-229-7429 } E-mail = tbudd-at-stlawu.edu } } This message is made of 100% recycled electrons!
-- Dr. T. Budd Chair of Biology St. Lawrence University Canton, NY 13617 Phone = 315-229-5640 Fax = 315-229-7429 E-mail = tbudd-at-stlawu.edu
may I take a few moments of your precious time? I should like to have access to two types of information you are likely to provide.
1) How to remove the paraffine around a cut from a biopsy in which the presence of silicone is expected? (our main concern is to avoid the migration of said silicone during the removal of the paraffine)
2) How to best perform a biopsy of a tissue containing silicone destined for study in a SEM? (we are especially looking for details regarding cutting and holding in place the silicone)
Thank you very much in advance, Yours respectfully,
I need some advice on cleaning a penning gauge. I have an Edwards Model CP 25-K, on a Cambridge 360 Stereoscan. There are some very stubborn deposits, especially on the three inserts. Any suggestions are appreciated
thanks.
Bob Kayton, PhD Histo/EM Core 503-494-2504-Lab 503-703-3938-Cell
Both the cups (Cathode cups, part number D145-33-007) and post (Anode, part number D145-33-006) can be replaced. They can also be cleaned by abrasion (SiC paper) and reused but beware that the anode has been surface treated and will not last very long after abrasion (about 6 months) so it is better to replace it. Parts from your local Edwards agent.
I use SiC paper and wash with alcohol afterwards for the cathodes and the body of the gauge, I only replace the cathodes when they are really shot. Note the orientation of the cathode cups when you take them out.
Good luck, Ron
On Thu, 19 Dec 2002 14:20:30 -0800 Robert Kayton {kayton-at-ohsu.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I need some advice on cleaning a penning gauge. I have an Edwards Model CP 25-K, on a Cambridge 360 Stereoscan. There are some very stubborn deposits, especially on the three inserts. Any suggestions are appreciated } } thanks. } } Bob Kayton, PhD } Histo/EM Core } 503-494-2504-Lab } 503-703-3938-Cell } } }
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Robert, If you have access to a bead blaster (sand blaster that uses fine glass beads), it's a lot less work than SiC. Any aluminum parts should be done with a reduced pressure of 35-40 psi or you may find that the aluminum disappears pretty quickly. If the stainless steel parts don't clean up at that pressure, you can go back up to 100 psi or so. Be sure to mask any sealing surfaces with masking tape before blasting. I don't believe there are any screw threads inside your gauge, but some have threaded parts. Male threads should be masked with masking tape and female threads should have a properly sized machine screw inserted to protect the threads.
Ken Converse owner Quality Images Third party SEM service Delta, PA
Robert Kayton wrote:
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Hello, I need a used specimen holder for Jeol 2010F TEM. Do you have any information? Thanks ************************************************************* Xuedong Bai School of Materials Science and Engineering Georgia Institute of Technology 771 Ferst Drive, N. W. Atlanta, GA 30332-0245 Phone: (404)385-0326 (O); (404)875-2099 (H) Fax: (404)894-9140 Email: xb8-at-mail.gatech.edu **************************************************************
I killed the anode on my Varian gauge by sanding it. Since that was happening, I am very careful with anodes. I would suggest to replace it if possible or try mild organic solvent to remove the build up. It looks like any mechanical treatment is not good for anodes. Sergey
At 08:13 AM 12/20/02 +0000, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Sergey Ryazantsev, Ph.D. Electron Microscopy Department of Biological Chemistry, School of Medicine University of California, Los Angeles Box 951737 Los Angeles, CA 90095-1737
Randy I am sorry for late comments. I agree with Wil Bigelow that LN2 trap will reduce the pump performance. If you need just to increase the system's overall performance, I would suggest you have to do very good service for it first (replace all suspicious O-rings, clean everything up). I highly recommend to use Santovac-5 DP (I assume, it's DP based system, because TP don't need LN2). When I come in UCLA, I had DV-502A vacuum system with 5*10-5 torr. I cleaned up DP (messy work and a lot of solvents), replaced all O-rings (the system was 10 y.o. at the moment) and used Santovac-5. Since that I do have 5*10-7 with no LN2 (and no further service for more than 5 years). As an alternative, you may install some "cold finger" with protective screens near your sample in the Bell Jar. You really need LN2 trap over DP if you pump a lot of water in your experiments. Sergey
At 06:09 PM 12/16/02 -0600, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Sergey Ryazantsev, Ph.D. Electron Microscopy Department of Biological Chemistry, School of Medicine University of California, Los Angeles Box 951737 Los Angeles, CA 90095-1737
If the cells are already fixed, you could scrub them from the surface and then concentrate by centrifugation. Surprisingly (to me) such terrible procedure does not affect the structure. Another way to do it "scientifically" - you may use tripsin to detach cells from the surface. It's the standard procedure in the cell-biology. They do have pre-made tripsin solution, you just substitute cultural media on tripsin solution for couple of minutes, discard tripsin and then resuspend cells in the fresh media. Sergey
At 11:37 AM 12/16/02 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Sergey Ryazantsev, Ph.D. Electron Microscopy Department of Biological Chemistry, School of Medicine University of California, Los Angeles Box 951737 Los Angeles, CA 90095-1737
I've inherited an old Voyager II (12 years old??) Sun System, I think. Looking for any remaining active users, list servers, user groups, and latent programers, extra parts, software, printers, accessories, etc. to support my future use of this system. Jeff/Texas wa5ekh-at-juno.com
Trypsinized cells round up and change morphology. If you're interested in morphology, you don't want to introduce a variable other than your experimental.
Also, I wouldn't soak cells for long times in sucrose. Aldehyde fixatives are somewhat reversible, especially formaldehyde. Lightly fixed cells sitting in aqueous solutions for a long time will change shape too. Infiltrate cells for ultracryotomy with 2.1M sucrose in PBS for 20-30 min and then freeze.
Sergey is right, you can fix the cells for a few minutes (10), scrape them up with a cell scraper, rubber policeman, or other scraper, pellet them, further fix them as a pellet (which helps them stick together) and then process them as a block. It might be necessary to encase the block in molten agar cooled to about 40 degrees to keep them together. Just don't fix the agar in glut or it will prevent further infiltration of other solutions.
For conventional microtomy, cells can be grown on films and the films sectioned or embedded in situ in dishes or culture slides and peeled up as a slab, as described here recently.
Season's greetings, Sara Miller
On Fri, 20 Dec 2002, Sergey Ryazantsev wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Ken, hello } } If the cells are already fixed, you could scrub them from the surface and } then concentrate by centrifugation. Surprisingly (to me) such terrible } procedure does not affect the structure. Another way to do it } "scientifically" - you may use tripsin to detach cells from the } surface. It's the standard procedure in the cell-biology. They do have } pre-made tripsin solution, you just substitute cultural media on tripsin } solution for couple of minutes, discard tripsin and then resuspend cells in } the fresh media. Sergey } } At 11:37 AM 12/16/02 +0100, you wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } } } -- } } Hi, } } I have been reading, with great interest, the replies to } } Jeannette Taylor's problem with embedding her cells. I encounter a } } similar problem when I try and flat embed cultured cells for } } cryo-ultramicrotomy. I have tried to grow Hela cells on both glass and } } plastic and then embed in 12% gelatin and infiltrate in 2.3M sucrose. The } } only time the cells have come away from the substrate, successfully, was } } when one of the samples was fixed heavily for morphological studies. If } } the samples are fixed lightly ie 2% para-formaldehyde, the cells remain } } firmly adhered to the substrate even after 2 weeks infiltration in sucrose! } } Has anyone had similar problems? Is there a simple answer to } } this? Answers on a Christmas card... } } } } } } Ken Blight } } Senior Scientific Officer } } Cancer Research UK } } London } } England } } ------------------------------------------------------ } } Sergey Ryazantsev, Ph.D. } Electron Microscopy } Department of Biological Chemistry, School of Medicine } University of California, Los Angeles } Box 951737 } Los Angeles, CA 90095-1737 } } (310) 825-1144 (office) } Pager: (310) 845-0248 } FAX: (310) 206-5272 (departmental) } mailto:sryazant-at-ucla.edu } } } }
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Have you verified that vacuum level with an independent, calibrated vacuum gauge? Vacuum sounds way to high for that system. I agree with all of your recommendations, but case in point, the vacuum you claim is on an order of what we normally find in a valve isolated electron gun (much smaller vacuum and seal volumes) pumped by a 20 L/sec ion pump. The vacuum level you stated for the start of your work is far closer to the best I have seen out of this particular evaporator after a complete rebuild.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174
On Friday, December 20, 2002 3:01 PM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Randy } I am sorry for late comments. I agree with Wil Bigelow that LN2 trap will } reduce the pump performance. If you need just to increase the system's } overall performance, I would suggest you have to do very good service for } it first (replace all suspicious O-rings, clean everything up). I highly } recommend to use Santovac-5 DP (I assume, it's DP based system, because TP } don't need LN2). When I come in UCLA, I had DV-502A vacuum system with } 5*10-5 torr. I cleaned up DP (messy work and a lot of solvents), replaced } all O-rings (the system was 10 y.o. at the moment) and used } Santovac-5. Since that I do have 5*10-7 with no LN2 (and no further } service for more than 5 years). As an alternative, you may install some } "cold finger" with protective screens near your sample in the Bell } Jar. You really need LN2 trap over DP if you pump a lot of water in your } experiments. Sergey } } At 06:09 PM 12/16/02 -0600, you wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hi All, } } I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to put a LN2 cold } } trap } } on it, in an attempt to clean up the vacuum a tad more. There is a 3.5 inch } } by 4.5 inch plate right above the diffusion stack which looks to be a great } } place to attach a cold trap. Rumor has it that this was an option. Are there } } any old traps kicking around that one can acquire? I could have our machine } } shop manufacture one, but was hoping for a cheaper route. } } Thanks in advance, } } Randy Nessler } } 319-335-8142 } } ------------------------------------------------------ } } Sergey Ryazantsev, Ph.D. } Electron Microscopy } Department of Biological Chemistry, School of Medicine } University of California, Los Angeles } Box 951737 } Los Angeles, CA 90095-1737 } } (310) 825-1144 (office) } Pager: (310) 845-0248 } FAX: (310) 206-5272 (departmental) } mailto:sryazant-at-ucla.edu } }
On Friday, December 20, 2002, at 04:58 AM, qualityimages wrote:
} If you have access to a bead blaster (sand blaster that uses fine } glass beads), it's a lot less work than SiC. Any aluminum parts } should be done with a reduced pressure of 35-40 psi or you may find } that the aluminum disappears pretty quickly.
} Ken Converse } Dear Ken and Robert, The instrument I used, called an "air eraser", offered a choice of glass beads, corundum, or, I think, starch grains (in any case, something pretty soft) to use as the abrading particles. The soft abraders could be useful for Al; however, the dirt may be tougher than the Al, making the process unsuitable. The air eraser is ~$100, and it's useful for a number of tasks. Yours, Bill Tivol EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
I'm don't know what the electrodes of a Penning gauge are made from, neither have I needed to try this on ours. But one reagent that is good for removing carbon or similar deposits is a solution of, say, 10% potassium hydroxide in ethanol or methylated spirits. Simply soak for half and hour, rinse with distilled water, and if necessary rub off with a very soft cloth.
However, this reagent goes like mad for aluminium (and makes some beautiful etch pits). But if all other liquid reagents fail, you might try this.
+-----------------------------------------+ Robert H.Olley J.J.Thomson Physical Laboratory University of Reading Whiteknights Reading RG6 6AF England +-----------------------------------------+ Phone: {direct line +44 (0) 118 9318572 {University internal extension 7867 Fax: +44 (0) 118 9750203 Email: R.H.Olley-at-reading.ac.uk URL: http://www.reading.ac.uk/~spsolley +-----------------------------------------+
Sergy, These are the ones I clean most often. Yes, the anodes must be treated carefully, as they are aluminum, but they also are subject to ion etching and will eventually "go away" just from use. I've found that when the central spindle loses about 1/3 of its diameter, they don't work so well any more and a new anode should be installed. If you have access to a machine shop, they're not too complicated to make. Last time I looked Varian wanted $180 for a cleaning kit that contained 1 spare anode and that was many years ago. Custom should be much cheaper.
Ken Converse owner Quality Images third party SEM service Delta, PA
Sergey Ryazantsev wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I killed the anode on my Varian gauge by sanding it. Since that was } happening, I am very careful with anodes. I would suggest to replace } it if possible or try mild organic solvent to remove the build up. It } looks like any mechanical treatment is not good for anodes. Sergey } } At 08:13 AM 12/20/02 +0000, you wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hi Robert, } } } } Both the cups (Cathode cups, part number D145-33-007) and } } post (Anode, part number D145-33-006) can be replaced. They } } can also be cleaned by abrasion (SiC paper) and reused but } } beware that the anode has been surface treated and will not } } last very long after abrasion (about 6 months) so it is } } better to replace it. Parts from your local Edwards agent. } } } } I use SiC paper and wash with alcohol afterwards for the } } cathodes and the body of the gauge, I only replace the } } cathodes when they are really shot. Note the orientation of } } the cathode cups when you take them out. } } } } Good luck, } } Ron } } } } On Thu, 19 Dec 2002 14:20:30 -0800 Robert Kayton } } {kayton-at-ohsu.edu} wrote: } } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } America } } } To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com } } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } -----------------------------------------------------------------------. } } } } } } } } } I need some advice on cleaning a penning gauge. I have an Edwards } } Model CP 25-K, on a Cambridge 360 Stereoscan. There are some very } } stubborn deposits, especially on the three inserts. Any suggestions } } are appreciated } } } } } } thanks. } } } } } } Bob Kayton, PhD } } } Histo/EM Core } } } 503-494-2504-Lab } } } 503-703-3938-Cell } } } } } } } } } } } } } ---------------------- } } Mr. R.C. Doole } } Department of Materials, } } University of Oxford. } } Parks Road, Oxford. OX1 3PH. UK. } } Phone +44 (0) 1865 273701 } } Fax +44 (0) 1865 283333 } } ron.doole-at-materials.ox.ac.uk } } http://www-em.materials.ox.ac.uk/ } } ********************************* } } } } We are pleased to host EMAG'03 in Oxford. 3rd to 5th September 2003. } } } } ******************************** } } } ------------------------------------------------------ } } Sergey Ryazantsev, Ph.D. } Electron Microscopy } Department of Biological Chemistry, School of Medicine } University of California, Los Angeles } Box 951737 } Los Angeles, CA 90095-1737 } } (310) 825-1144 (office) } Pager: (310) 845-0248 } FAX: (310) 206-5272 (departmental) } mailto:sryazant-at-ucla.edu } }
I have used this technique to clean a penning guage and other parts (but NEVER use it on gun parts in microscopes) and all the precautions listed are to be taken. However, after using glass beading the first time, I think that it gets dirty faster and the new texture of the metal surface makes it harder to clean any other way. Watch out using higher pressures as the metal can become deformed very easily.
DV502A has 800 l/sec DP!!! It enough to pump down a huge volume. If you don't have any leaks in your system, the vacuum would be directly dependent from the ultimate vacuum for the pump (which is somehow dependent from pump's actual construction/quality and DP Oil). In most cases leaks are the reason of vacuum degradation. Any normally serviced vacuum evaporator should deliver at least very good 10-6 torr. 10-5 is very bad and actually you may not use it for biological sample preparation. In my particular case, I've replaced ALL O-rings on viton with touch of Apiezon L, cleaned and polished DP and used Santavac-5. Santavac-5 is great DP oil. You have to try. On my DV502A I have 2*10-6 after about 40 min pumping (no LN2) and better than 5*10-7 overnight. I am using MKS cold cathode gauge with sensitivity up to 10-9 torr (which I don't need). I think, the vacuum quality is mostly a function of how clean your system and how many 10-year old cracked "buna" O-rings is inside your system. The problem with "buna" - it does not hold the shape and easily deformed even if it's not old. It's also destroyed by vacuum grease (does not matter what manufacturers told you). Viton is much better. Since I spent $500 on Santavac-5 8 years ago, I never touch my DP again. By the way, I have another vacuum system, which I build by myself. It has exact the same volume and similar amount of O-rings but 400 l/s TP from SEIKO. That system is oil-free. I mean, it has scroll pump as a backing device and TP itself does not have any oil (it's magnetically levitated beauty). So, when I build the system, I was expecting similar productivity for this system as for DV502A but oil-free. I was completely wrong! This "baby" easily delivered to me 5*10-7 torr in 20 (yes, twenty) min! After a few hours, it's going into 10-8. So, my "theory" is that in the standard setup, oil from mechanical pump contaminated the whole system (and your sample!) and adsorbs a lot of air, which slowly released during the high vacuum pumping cycle. Because my new system is oil-free, it's much faster. It has 12x12" Bell Jar with 6' collar. Another example: my old Polaron with 100 l/s DP (Santavac-5 again) and 12x12" Bell Jar. This system is very comfortable with 2*10-6 (no LN2) after I repaired manufacturers defect in DP. All tricks how to get good vacuum perfectly described in the Wil Bigelow book. Have a great Holidays (don't start cleaning DP- it's messy)! Sergey
At 08:13 PM 12/20/02 -0600, you wrote: } Sergey, } } Have you verified that vacuum level with an independent, calibrated vacuum } gauge? Vacuum sounds way to high for that system. I agree with all of } your recommendations, but case in point, the vacuum you claim is on an } order of what we normally find in a valve isolated electron gun (much } smaller vacuum and seal volumes) pumped by a 20 L/sec ion pump. The vacuum } level you stated for the start of your work is far closer to the best I } have seen out of this particular evaporator after a complete rebuild. } } Allen R. Sampson } Advanced Research Systems } 317 North 4th. Street } St. Charles, Illinois 60174 } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com } } } On Friday, December 20, 2002 3:01 PM, Sergey Ryazantsev } [SMTP:sryazant-at-ucla.edu] wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Randy } } I am sorry for late comments. I agree with Wil Bigelow that LN2 trap will } } reduce the pump performance. If you need just to increase the system's } } overall performance, I would suggest you have to do very good service for } } it first (replace all suspicious O-rings, clean everything up). I highly } } recommend to use Santovac-5 DP (I assume, it's DP based system, because } TP } } don't need LN2). When I come in UCLA, I had DV-502A vacuum system with } } 5*10-5 torr. I cleaned up DP (messy work and a lot of solvents), } replaced } } all O-rings (the system was 10 y.o. at the moment) and used } } Santovac-5. Since that I do have 5*10-7 with no LN2 (and no further } } service for more than 5 years). As an alternative, you may install some } } "cold finger" with protective screens near your sample in the Bell } } Jar. You really need LN2 trap over DP if you pump a lot of water in your } } experiments. Sergey } } } } At 06:09 PM 12/16/02 -0600, you wrote: } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } Hi All, } } } I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to put a LN2 } cold } } } trap } } } on it, in an attempt to clean up the vacuum a tad more. There is a 3.5 } inch } } } by 4.5 inch plate right above the diffusion stack which looks to be a } great } } } place to attach a cold trap. Rumor has it that this was an option. Are } there } } } any old traps kicking around that one can acquire? I could have our } machine } } } shop manufacture one, but was hoping for a cheaper route. } } } Thanks in advance, } } } Randy Nessler } } } 319-335-8142 } } } } ------------------------------------------------------ } } } } Sergey Ryazantsev, Ph.D. } } Electron Microscopy } } Department of Biological Chemistry, School of Medicine } } University of California, Los Angeles } } Box 951737 } } Los Angeles, CA 90095-1737 } } } } (310) 825-1144 (office) } } Pager: (310) 845-0248 } } FAX: (310) 206-5272 (departmental) } } mailto:sryazant-at-ucla.edu } } } }
Sergey Ryazantsev, Ph.D. Electron Microscopy Department of Biological Chemistry, School of Medicine University of California, Los Angeles Box 951737 Los Angeles, CA 90095-1737
That is a strong pump for this system - the normally configured system uses a 250 L/sec pump. A diffusion pump, as any pump, is a differential system - The ultimate pressure achieved is a balance between the leak rate in the input, the back pressure in the output and the bypass rate in the pump (how much gas can pass from the output to the input for a given pressure differential, giving rise to the mechanical pump oil contamination in a system). Although these pumps are rated up to 10-9 pressures, this is never seen in operational systems. If you were to cap the diffusion pump with a metal sealed, solid metal cap and had an enormous backing pump, you may come close to the rated ultimate rating. In practical systems, these levels are but a dream.
I'd be interested in knowing what roughing pump you are using.
No vacuum system has no leaks, particularly when elastomeric seals are used. The standard Denton, with its manual valves and bell jar seal, has a lot of elastomeric seals. And a cold cathode vacuum gauge accuracy is debatable at 10-6 or less.
I've only recommended and used Santovac 5 for over twenty years. It is a great diffusion pump oil. My main reason for recommending it is not the ultimate vacuum attainable (debatable considering the greater temperature apparently needed by Santovac, although I've never had a problem using it in any diffusion pump), but rather it's relative insensitivity to sudden air inrushes when hot. That means that it will 'crack' and polymerize less than other oils. In other words - it will last longer and cause less hard deposits on the pump.
As far as o-rings, I've found Buna to be quite acceptable, lightly coated with Brayco, for static seals. Where dynamic seals are used (rotational or translational forces are common), I use Buna with Apiezon (the waxier Apiezon has more staying power, although it will also hold particulate contaminants more). The reason for cracked o-rings is neglect, not the suitability of vacuum greases. The reason for greasing o-rings is to provide a light coating that preserves the qualities of the elastomeric material, not to make up for insufficiencies in the vacuum sealing surfaces. In this respect, the better vacuum greases do a good job and do not compromise either Buna or Viton. I have many 25+ year old systems that have original o-rings that are indistinguishable from new, in appearance, shape or conformance.
The deformability of Buna is actually a plus, at least in systems that are mostly held at vacuum. You may have noticed that a system that was just rebuilt with new or rebuilt o-rings takes some time to come to an ultimate vacuum equilibrium. That, of course, involves the outgassing of the system components after being exposed to atmosphere for some time during the rebuild. But it also includes the time required for the elastomeric vacuum seals to be 'sucked' into place. A well designed o-ring seal will depend on the mechanical pressures on the o-ring, but will ultimately depend on the o-ring's conformance under the gas pressures it's subjected to.
In my experience, the Buna o-rings will deform to provide a good seal faster and, properly maintained, will continue to conform to a shape that best seals. I generally use Viton for it's improved resistance to high temperatures. In either case, I use an acetone wipe for cleaning o-rings every time I recondition them. It tends to swell the o-rings with two effects - it restores them to their original shape and helps to provide a quick seal when the system is pumped down again.
BTW, I've cleaned more DPs and refurbished more vacuum systems than I'd like to elucidate. In my business, I tend to get the instruments that the manufacturers don't service anymore, didn't service properly or have been neglected for some time.
Just a few ruminations from a long career of servicing many vacuum instruments.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, Illinois 60174
On Sunday, December 22, 2002 3:12 AM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu] wrote: } Allen } } DV502A has 800 l/sec DP!!! It enough to pump down a huge volume. If you } don't have any leaks in your system, the vacuum would be directly dependent } from the ultimate vacuum for the pump (which is somehow dependent from } pump's actual construction/quality and DP Oil). In most cases leaks are the } reason of vacuum degradation. Any normally serviced vacuum evaporator } should deliver at least very good 10-6 torr. 10-5 is very bad and actually } you may not use it for biological sample preparation. In my particular } case, I've replaced ALL O-rings on viton with touch of Apiezon L, cleaned } and polished DP and used Santavac-5. Santavac-5 is great DP oil. You have } to try. On my DV502A I have 2*10-6 after about 40 min pumping (no LN2) and } better than 5*10-7 overnight. I am using MKS cold cathode gauge with } sensitivity up to 10-9 torr (which I don't need). I think, the vacuum } quality is mostly a function of how clean your system and how many 10-year } old cracked "buna" O-rings is inside your system. The problem with "buna" } - it does not hold the shape and easily deformed even if it's not old. It's } also destroyed by vacuum grease (does not matter what manufacturers told } you). Viton is much better. Since I spent $500 on Santavac-5 8 years ago, } I never touch my DP again. By the way, I have another vacuum system, which } I build by myself. It has exact the same volume and similar amount of } O-rings but 400 l/s TP from SEIKO. That system is oil-free. I mean, it } has scroll pump as a backing device and TP itself does not have any oil } (it's magnetically levitated beauty). So, when I build the system, I was } expecting similar productivity for this system as for DV502A but } oil-free. I was completely wrong! This "baby" easily delivered to me } 5*10-7 torr in 20 (yes, twenty) min! After a few hours, it's going into } 10-8. So, my "theory" is that in the standard setup, oil from mechanical } pump contaminated the whole system (and your sample!) and adsorbs a lot of } air, which slowly released during the high vacuum pumping cycle. Because } my new system is oil-free, it's much faster. It has 12x12" Bell Jar with } 6' collar. Another example: my old Polaron with 100 l/s DP (Santavac-5 } again) and 12x12" Bell Jar. This system is very comfortable with 2*10-6 } (no LN2) after I repaired manufacturers defect in DP. All tricks how to get } good vacuum perfectly described in the Wil Bigelow book. Have a great } Holidays (don't start cleaning DP- it's messy)! Sergey } } } At 08:13 PM 12/20/02 -0600, you wrote: } } Sergey, } } } } Have you verified that vacuum level with an independent, calibrated vacuum } } gauge? Vacuum sounds way to high for that system. I agree with all of } } your recommendations, but case in point, the vacuum you claim is on an } } order of what we normally find in a valve isolated electron gun (much } } smaller vacuum and seal volumes) pumped by a 20 L/sec ion pump. The vacuum } } level you stated for the start of your work is far closer to the best I } } have seen out of this particular evaporator after a complete rebuild. } } } } Allen R. Sampson } } Advanced Research Systems } } 317 North 4th. Street } } St. Charles, Illinois 60174 } } } } phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com } } } } } } On Friday, December 20, 2002 3:01 PM, Sergey Ryazantsev } } [SMTP:sryazant-at-ucla.edu] wrote: } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FA Q.html } } } -----------------------------------------------------------------------. } } } } } } } } } Randy } } } I am sorry for late comments. I agree with Wil Bigelow that LN2 trap will } } } reduce the pump performance. If you need just to increase the system's } } } overall performance, I would suggest you have to do very good service for } } } it first (replace all suspicious O-rings, clean everything up). I highly } } } recommend to use Santovac-5 DP (I assume, it's DP based system, because } } TP } } } don't need LN2). When I come in UCLA, I had DV-502A vacuum system with } } } 5*10-5 torr. I cleaned up DP (messy work and a lot of solvents), } } replaced } } } all O-rings (the system was 10 y.o. at the moment) and used } } } Santovac-5. Since that I do have 5*10-7 with no LN2 (and no further } } } service for more than 5 years). As an alternative, you may install some } } } "cold finger" with protective screens near your sample in the Bell } } } Jar. You really need LN2 trap over DP if you pump a lot of water in your } } } experiments. Sergey } } } } } } At 06:09 PM 12/16/02 -0600, you wrote: } } } } ------------------------------------------------------------------------ } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } -----------------------------------------------------------------------. } } } } } } } } } } } } Hi All, } } } } I have a Hitachi HUS-4 vacuum evaporator. I'm hoping to put a LN2 } } cold } } } } trap } } } } on it, in an attempt to clean up the vacuum a tad more. There is a 3.5 } } inch } } } } by 4.5 inch plate right above the diffusion stack which looks to be a } } great } } } } place to attach a cold trap. Rumor has it that this was an option. Are } } there } } } } any old traps kicking around that one can acquire? I could have our } } machine } } } } shop manufacture one, but was hoping for a cheaper route. } } } } Thanks in advance, } } } } Randy Nessler } } } } 319-335-8142 } } } } } } ------------------------------------------------------ } } } } } } Sergey Ryazantsev, Ph.D. } } } Electron Microscopy } } } Department of Biological Chemistry, School of Medicine } } } University of California, Los Angeles } } } Box 951737 } } } Los Angeles, CA 90095-1737 } } } } } } (310) 825-1144 (office) } } } Pager: (310) 845-0248 } } } FAX: (310) 206-5272 (departmental) } } } mailto:sryazant-at-ucla.edu } } } } } } } } ------------------------------------------------------ } } Sergey Ryazantsev, Ph.D. } Electron Microscopy } Department of Biological Chemistry, School of Medicine } University of California, Los Angeles } Box 951737 } Los Angeles, CA 90095-1737 } } (310) 825-1144 (office) } Pager: (310) 845-0248 } FAX: (310) 206-5272 (departmental) } mailto:sryazant-at-ucla.edu } } }
With the correct choice of abrasive size and hardness, and the right choice of operating pressure and distance from the nozzle, abrasive cleaners can be helpful tools. However, in using one you should be aware that this microscopic "shot peening" of the surface has other effects on metals, including work hardening and distortion.
For example, in microelectronics bright-plated gold is notoriously hard to form a reliable polymer bond to. To overcome this problem I once worked with a process that required micro-abrasive roughening of the plating surface in the bottom of a flat Kovar package about the size of a matchbook. In the course of 30 seconds of abrasive blasting, the thin bottom of this package would acquire a visible bow due to its lateral expansion within the confining sidewalls.
It may seem counter-intuitive, but the direction of the bow was toward the abrasive jet rather than away from it, due to expansion of that surface relative to the unabraded rear.
John Twilley Conservation Scientist
Bill Tivol wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } On Friday, December 20, 2002, at 04:58 AM, qualityimages wrote: } } } If you have access to a bead blaster (sand blaster that uses fine } } glass beads), it's a lot less work than SiC. Any aluminum parts } } should be done with a reduced pressure of 35-40 psi or you may find } } that the aluminum disappears pretty quickly. } } } Ken Converse } } } Dear Ken and Robert, } The instrument I used, called an "air eraser", offered a choice of } glass beads, corundum, or, I think, starch grains (in any case, } something pretty soft) to use as the abrading particles. The soft } abraders could be useful for Al; however, the dirt may be tougher than } the Al, making the process unsuitable. The air eraser is ~$100, and } it's useful for a number of tasks. } Yours, } Bill Tivol } EM Scientist and Manager } Cryo-Electron Microscopy Facility } Broad Center, Mail Code 114-96 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu
We wanted to thank everyone who sent in suggestions for "problem nerves". We ended up having very good luck with Karen Bentley's suggestion.
We cut at 1000nm, place the section on a drop of water, and then place a drop of 100% xylene on top. We then drain the slide and let it dry upright, & then put it on the hot plate to stain.
The doctors are very pleased with our new and improved results!
Thanks again---- The techs. at Nebraska Health System
} } Hello } } } } Can anyone give me information on how to prepare good CPD arabidopsis } } roots and hypocotyls for FESEM. } } After fixation in 4% formaldehyde in 0.5x PEM buffer (Pipes,EGTA,MgSO4), } } samples were washed in 0.5x PEM buffer,cryoprotected in DMSO 25% and } } 50%,cryosectioned by 120°C, thawed in DMSO 50%. Extraction was done } } with 3% } } sodium hypochlorite and 0.1 % pectolyase. Dehydration in 30% ....100% } } ethanol and finally CPD (Balzers CPD 010) with liquid CO2 (10 changes } } each 3 min and another 20 changes each 7 min.) } } The cellulose fibrils are nice but the cell walls are not flat. A LOT of } } FOLDS are visible. I guess this must be an artefact from CPD. } } } } Any suggestions for good CPD methods on plant tissue and especially on } } extracted plant tissue are welcome } } } } } } Thanks in advance } } } } S. Foubert
Dear listers, I have a friend who has about 10 customers looking for a digital image capture system for their SEMs. I know several vendors are frequenters of this list, but I'm not at all sure that it's inclusive. Does anyone have a really complete list of digital image capture vendors, perhaps even broken down by active vs. passive systems?
Thanks, Ken Converse owner Quality Images third party SEM service Delta, PA
Hi, Well the first thing I would do would be to get rid of the EGTA in your fixation fixation buffer. Calcium generally strengthens plant structure. Of course you are going to extract your pectins later, so this might not make much difference. But it will simplify things.
EGTA got put into fixation buffers for fixing plant cells in the mistaken idea that it 'stabilizes' microtubules. But since papers reporting tubulin biochemistry in vitro include EGTA in buffers to show dilution-induced depolymerization, EGTA can hardly be said to stabilize microtubules. Certainly not in the way in which taxol stabilizes microtubules. Furthermore, Calcium is a component of membranes and when you extract it at the time of fixation you damage your membranes at the same time as fixation, which tends to be bad news for good structural preservation. If you want to extact calcium to take out pectins, the time to do that is after fixation.
Honestly, this probably won't help your folding problem. But it might, and it will help other fixations if you do them in the presence of EGTA.
Good luck, Tobias Baskin
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It depends on what you mean by "unfix" and what you want to do with the tissue after you unfix it.
Aldehydes are somewhat reversible and a portion can can simply be washed out. This is why you don't want to leave tissue in buffer after aldehyde fixation, but can store it in glut for a while without damaging ultrastructure.
Aldehydes can also be "quenched" by certain agents such as ammonium chloride or glycine for immunostaining the tissue.
Some tissues actually work better in some immunostains if they are fixed because some antibodies are made with denatured proteins.
If you mean "completely return the tissue to its unfixed state", I'm afraid the answer is "no".
Sara Miller
On Mon, 30 Dec 2002, Beveridge, Mark J. wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } i would like to know if there is any way to unfix tissue fixed in } paraformaldehyde for immunoblotting? } } }
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
Have any of you successfully connected a Polaroid DMC2 camera to an Apple PowerBook via a Belkin firewire to scsi converter running on OS X and OS 9 via Photoshop 6?
Thank you for your assistance. Bless wishes for a healthy and safe 2003!
Ken
--------------------------------------- Kenneth L, Tiekotter, Adjunct Professor Dept. of Biology The University of Portland 5000 N Willamette Blvd, Portland, OR 97203 USA
We have a researcher on campus who would like to examine the cell size of 3000 year old wood. Any ideas on the sample preparation protocol to preserve the cell structure without shrinking?
Please direct your responss to Cheryl Jensen (jensenc-at-missouri.edu).
Thanks in advance. Lou Ross -- Senior Electron Microscope Specialist Electron Microscopy Core Facility W136 Veterinary Medicine University of Missouri Columbia, MO 65211-5120 (573) 882-4777, fax 884=5414 email: rosslm-at-missouri.edu web: www.biotech.missouri.edu/emc
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