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From: abijohn-at-tiscali.co.uk
Date: Fri, 1 Aug 2003 12:21:46 +0100
Subject: please acknowledge receipt

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Garry

The H7000 that I use has another exposure meter display inside the column just behind the screen. this consists of two red LEDs (ie no green middle. If the two come on together that is a correct exposure but increasing or decreasing the beam density on the screen should make one or the other come on. I apologise if you have checked this but if the column meter works then it sounds like it is the other meter. Also have you checked that no one has 'mucked about' with the meter calibration settings.

If everything else checks out then maybe it's just the link from the central screen that has broken - remember this is a current density rather than light meter.

I hope this helps.

Malcolm

Malcolm Haswell
e.m. unit
Chemispec
School of Health, Natural and Social Sciences
Fleming Building*
University of Sunderland
Tyne & Wear
SR1 3SD
UK
tel no: 0191 515 2872 / 3468
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
} From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}


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communicate with you. This is important as we would
Have to talk about the modalities of the transaction.
Waiting to hear from you,
Abiodun John


please do reply to my alternate and secure email abiodunjohn-at-lycos.co.uk




From daemon Fri Aug 1 06:44:20 2003



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Fri, 1 Aug 2003 08:34:55 ADT
Subject: re:pepper inthin sections

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Hi Randy
Do you have the same problem when you use gold or nickel grids?
Some time ago I worked on immunogold technique in tissue and I
had problem with pepper/dirt film over the sections. I used nickel
grids that were bought ages ago. I bought a new batch, cleaned it
and sections were clean, no pepper/dirt film.
Dorota


From daemon Fri Aug 1 14:01:58 2003



From: Ann St. Amand :      astamand-at-phycotech.com
Date: Fri, 01 Aug 2003 14:38:01 -0400
Subject: LM, need help with autofluorescence of algae from humic

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Hi all, I have 2 standard cubes I use (blue: excites at 490, emits at 515,
for chlorophyll a, and green: excites at 545 and emits at 590 for
phycoerythrin/cryptomonads), plus I have an additional cube for UV that I
use for DAPI and calcafluor white. My problem is taxa that are from humic
stained lakes. Does anyone have a better Excite/Emission range suggestion
for these systems, especially for phycoerythin or cryptomonads? Pigments
are measuring high, cells look healthy and are well colored, but fluoresce
poorly. I think the pigments have shifted their absorption ranges, rather
than the humics interfering (The preps I'm working with are very thin, with
no overlying water). This has been troublesome for a while, but lately, I
seem to be getting lots of samples from humic systems and I can ID and
count much faster if I have good, active fluorescence. I'll post responses
to the list if folks are interested. Thanks! Ann.

Ann St. Amand, Ph.D.
President
PhycoTech, Inc.
620 Broad St., Suite 100
St. Joseph, Michigan 49085 USA

269-983-3654 (voice)
269-983-3653 (fax)
mailto:astamand-at-phycotech.com
www.phycotech.com

Specializing in Aquatic Sample Analysis and Microscope Accessories

Director, Region V, North American Lake Management Society, www.nalms.org




From daemon Fri Aug 1 14:05:52 2003



From: Laura Torchin :      lrtorchin-at-ucdavis.edu
Date: Fri, 01 Aug 2003 11:43:35 -0700
Subject: embedding arabidopsis

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Hello,

I am having some trouble embedding arabidopsis seedling for TEM. I am
using epon-araldite mixture and my tissue just dissolves away upon
sectioning of my samples. I am assuming it is an embedding problem. I
dehydrate my samples using an ETOH gradient then switch to 100% propylene
oxide. I embed with a 2:1 (PO:epon-araldite) mixture for 12 hours then a
1:2 mixture for 12 hours then 100% epon-araldite at room temp followed by
24 hours at 30 degrees then 48 hours at 45 degrees. Should I change
something in my protocol or is there another embedding medium that may work
better for arabidopsis seedlings? Any help would be appreciated. Thankyou.

Laura Torchin
UCDavis Dehesh Lab
530-752-8190



From daemon Fri Aug 1 14:47:54 2003



From: Microshaw-at-aol.com
Date: Fri, 1 Aug 2003 15:38:24 EDT
Subject: Re: teaching 5th graders

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Becky-
I gave a microscope talk to my daughter's 4th grade class. On a Friday, I
gave the teacher 24 plastic petri dishes, one for each student and the teacher as
well. Then I had my daughter collect them on Monday. The rule was that - 1)
no liquids, and 2) each dish had to be scotch-taped closed, with only the
student's name on the label. The kids were free to collect a small specimen of
anything animal (not living), vegetable, or mineral. I then made slides of
each item, and tried to guess what each was. Some were obvious. I came back
with my microscope, and the slides, and made handouts for each child (and the
teacher). The 24 page handout had a microphotograph of each slide and the
student's name under the relative picture. The hand out also had a diagram of the
microscope, and some brief information (4th grade level). I let the students
come up one, by one to look through the microscope at this or that more
interesting specimen. To save money on the booklet, I made two sets of color prints:
One booklet contained all color photos (for the teacher's copy) and I Black
and White photocopied the book (in photo-mode) for the other 22 student
copies. I used gluestick to glue an original color print of each student's specimen
in their respective booklet, and handed them out accordingly. It was great
fun, and it worked out perfectly, and didn't take up too much of the teacher's
time. Total was about an hour total class time. The students were very
creative in their choices, and brought a full spectrum of things microscopic into
the class!
Rgds,
Mike Shaw

{ { Email: hoganbecky-at-hotmail.com
Name: Becky Hogan

Organization: Summerville Elementary

Education: K-8 Grade Grammar School

Location: Summerville, South Carolina

Question: I will be teaching gifted 5th graders in a new math/science
program this year and need to know what type of microscope would be best for
my program. Also, where can I buy a microscope for a reasonable price? Do
colleges ever donate used microscopes to schools? I am working with a
shoestring budget of $0.00 so I will have to make a personal purchase in order for my
students to use this wonderful teaching tool.
Thank you for asssisting me with this matter. I want to offer the
best science program possible!
Becky Hogan } }


From daemon Fri Aug 1 16:53:51 2003



From: sconnell-at-liai.org (by way of MicroscopyListServer)
Date: Fri, 1 Aug 2003 16:41:42 -0500
Subject: Ask-A-Microscopist: confocal microscopy land for the first time

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sconnell-at-liai.org) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
August 1, 2003 at 15:58:16
---------------------------------------------------------------------------

Email: sconnell-at-liai.org
Name: samuel connell

Organization: La Jolla Institute for Allergy and Immunology

Education: Graduate College

Location: San Diego, CA

Question: Iím heading into confocal microscopy calcium flux land for
the first time. Iíve done a fair amount in flow cytometry using
Indo-1. Iím interested in speaking with someone who has experience
in doing this work with visible wavelength probes, preferably with
the BioRad MRC 1024 system. Our system has the Kr/Ar mixed gas with
the 488, 568, and 647 laser lines available. Weíre planning on
looking at 512x512 XY images over time, potentially with scans every
5-10 seconds over 10-20 minutes or so. If someone would be so kind
as to touch base with me, I would be truly indebted for the shared
expertise.
Thanks,
--
Samuel Connell



---------------------------------------------------------------------------


From daemon Fri Aug 1 20:59:37 2003



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Fri, 1 Aug 2003 21:48:57 -0400 (EDT)
Subject: Re: Ask-A-Microscopist: confocal microscopy land for the first time

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We've done some work with Ca green. Basically, it gets brighter and
dimmer depending on the free Ca. It works with blue excitation. Not
particulary quantitative, but it gets the point across.

___________________________________
WORK: http://www.aecom.yu.edu/aif/




From daemon Sat Aug 2 02:04:38 2003



From: bant-at-gte.net
Date: Sat, 2 Aug 2003 15:55:51 +0900
Subject: Re: H

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From daemon Sat Aug 2 02:04:43 2003



From: bmmartin-at-erols.com
Date: Sat, 2 Aug 2003 14:58:44 +0800
Subject: gates

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From daemon Sat Aug 2 06:58:21 2003



From: Ralph Haswell :      s.f.haswell-at-planet.nl
Date: Sat, 02 Aug 2003 13:25:04 +0200
Subject: TEM - vacuum transfer holders

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Dear All,

I know that Gatan makes various vacuum transfer holders for the TEM but are
there any other suppliers?

Many thanks,

Ralph Haswell
Research Scientist
Shell International Chemicals B.V.
Shell Research & Technology Centre, P.O. Box 38000, 1030 BN Amsterdam, The
Netherlands




From daemon Mon Aug 4 04:40:48 2003



From: Zimmermann, Achim :      Achim.Zimmermann-at-jenoptik.com
Date: Mon, 4 Aug 2003 11:20:49 +0200
Subject: RE: Bayer filter versus 3CCD camera

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Hi Peter,

I really am wondering why nobody has raised this important question earlier.
Yes, RGB color interpolation with Bayer filter cameras can become an
problematic factor when it comes to quantitative spatial measurements of
coloured samples. Especially when working with low power magnification where
camera resolution is crucial.

Regarding Bayer filter cameras, you might want to consider the pixel
shifting technology used in the ProgRes C14 digital microscopy camera from
JENOPTIK Laser, Optik, Systeme GmbH.

The ProgRes C14 also uses a single CCD with Bayer filter design. The sensor
can be moved by means of a piezoelectric shifting device as many other pixel
shifting cameras use.

But there are different ways to shift the pixels to produce new information.
Most pixel shifting camera use the sensor shifting only to gain optical
resolution, i.e. scanning the image more accurately, which makes much sense
when using high resolution optics. Thereby the sensor is shifted in
fractions of the pixels' spacing, producing a slight overlap of the pixels.
But: quantitative spatial measurements might become problematic due to the
RGB color interpolation, where some spatial resolution is defintively lost.

In contrast to some other pixel shifting cameras, JENOPTIK's ProgRes C14
also shifts the sensor in full pixel steps, where the pixels overlap 100%.
Doing this at least four times in x- and y- direction you sample the full
RGB color information for each single pixel position. In the result you get
a true color RGB image without interpolation, comparable to a RGB filter
wheel camera. The file size (and thus nominal image resolution) stays the
same. But precise quantitative spatial measurements of colored samples is
now possible. We call this technique "Color-Co-Site-Sampling" and as far as
I know only ZEISS's Axicam HRc and JENOPTIK's ProgRes C14 do use this
technique in micro-photography.

You can combine this full pixel shifting increasing "color resolution" also
with fractional pixel shifting increasing spatial resolution.

An image says more than a thousand words - so if you want some illustration
for this technique please contact me.

The benefit of this technique compared to a filter wheel camera is, that you
can use the camera also for moving specimens in single shot mode, where
colors then are interpolated. And compared to a 3 CCD camera, the Bayer
filer camera is more light sensitive, because incoming light does not need
to be split up by a prism directing it onto the three single CCDs.

Of course, also in pixel shifting cameras pixel misalignment can occur - in
principle. You need to keep in mind that the sensor shifting has do be done
very precisely in the sub-µm range. To guarantee optimum alignment, the
ProgRes C14 comes with a built-in calibration procedure that allows the user
to calibrate the piezo scanner for best results.

I hope this information does add a new facette to your discussion about
quantitative spatial measurements of coloured samples with Bayer filter
cameras.

Best regards,

Achim.







===============================================
Achim Zimmermann
Product Manager Microscopy Cameras

JENOPTIK Laser, Optik, Systeme GmbH
Business Unit Sensor Systems - Digital Cameras
Göschwitzer Str. 25
D-07745 Jena
Germany

Tel.: +49 36 41 65 - 21 39 (office)
Tel.: +49 17 33 99 35 45 (mobile)
Fax: +49 36 41 65 - 21 44

eMail: achim.zimmermann-at-jenoptik.com
Web: www.progres-camera.com - www.jenoptik.com
===============================================



} -----Original Message-----
} From: Peter Van Osta [mailto:pvosta-at-unionbio-eu.com]
} Sent: Thursday, July 24, 2003 10:52 AM
} To: MSA
} Subject: Bayer filter versus 3CCD camera
}
---------.
}
}
} Hi,
}
} The two main types of color cameras used in microscopy I know
} of are the
} ones with 3 separate CCDs for each of the three primary colors and the
} ones with a Bayer filter design. In the 3CCD camera the
} spatial sampling
} is the same for each of the three colors, but not in the Bayer filter
} camera design ? How do you deal with this difference when doing
} quantitative spatial measurements of colored samples in microscopy ?
}
} What is the relative actual resolution seen with a 3CCD color
} camera and
} a camera with Bayer filter reconstruction? How to take into
} account the
} reduced sensitivity of the Bayer filter type for each color, compared
} with the 3CCD camera if any ? When are Bayer filter type
} cameras a valid
} alternative for a 3CCD camera in microscopy, besides the price ?
}
} What about pixel shifts in a 3CCD camera due to misalignment of the
} color filters/splitter and the CCDs ?
}
} Best regards,
}
} Peter Van Osta
}
} Union Biometrica N.V./S.A.
} European Scientific Operations (ESO)
} Cipalstraat 3
} B-2440 Geel
} Belgium
} Tel.: +32 (0)14 570 619
} Fax.: +32 (0)14 570 621
}
} http://www.unionbio.com/
}
} http://ourworld.compuserve.com/homepages/pvosta/cvwww.htm
}


From daemon Mon Aug 4 09:25:29 2003



From: Markus Doeblinger :      markus.doeblinger-at-materials.oxford.ac.uk
Date: Mon, 04 Aug 2003 15:23:02 +0100
Subject: TEM: maintenance of low voltage ion mills

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Hi,
My department is currently considering to buy a low voltage ion mill.
The users I've spoken to up to date all agree concerning the good
quality of the samples. However, the maintenance of many low energy ion

mills seems be a major problem, especially in the case of a large number

of users. We are also concerned about technical support from suppliers
located overseas.
Does anybody have experience with suppliers like Fischione and South Bay

Technology with respect to maintenance and technical support in Europe?
Certainly, I am also interested in comments on the effectiveness of low
energy ion mills in general.

Best regards,
Markus Doeblinger




From daemon Tue Aug 5 22:27:51 2003



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From daemon Wed Aug 6 04:19:00 2003



From: PRINCE JOHNSON. :      princej-at-myself.com
Date: Wed, 6 Aug 2003 16:16:36 +0000
Subject: ASISTANCE

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DEAR SIR,

FIRST AND FOREMOST,I MUST SOLICIT YOUR STRICTEST CONFIDENCE IN THIS TRANSACTION AND I PRAY THAT MY DECISION TO CONTACT YOU WILL BE GIVEN GENUINE APPROVAL CONSIDERING THE FACTS WE HAVE NOT KNOWN EACH OTHER BEFORE, I WISH TO USE THIS OPPORTUNITY TO INTRODUCE MYSELF TO YOU.

I AM PRINCE JOHNSON FROM LIBERIA,WEST AFRICA. I WRITES TO INFORM YOU MY DESIRE TO INVEST,AND TO BUY A LIVING HOUSE IN YOUR COUNTRY. I AM THE FIRST SON OF MR. WEAH JOHNSON, HE WAS A DIAMOND/GOLD MERCHANT IN MY COUNTRY.MY FATHER HAD A BULLET SHOT BY THE REBELS ON HIS WAY TRAVELLING OUT OF MY COUNTRY WITH TWO OF MY YOUNGER SISTER'S DUE TO PRESENT CRISIS THAT IS OCCURING IN MY COUNTRY(LIBERIA).MY SISTER'S DIED ON THE SPOT WHILE U.N.PEACE KEEPING FORCE RESCUED MY FATHER,HE WAS TAKEN TO HOSPITAL FOR MEDICAL TREATMENT WHICH HE LATER DIED. BEFORE HE DIED HE REAVEALED TO ME AND MY MOTHER ABOUT THE BOXES CONTAINING $8 MILLION US DOLLARS.WHICH HE DEPOSITED WITH A SECURITY COMPANY IN GHANA FOR SAFE KEEPING. MY FATHER DID NOT DISCLOSE THE CONTENT OF THE BOXES TO THE SECURITY COMPANY.TO AVIOD THE OFFICIALS FROM RAISING EYE BROWS TO THE FUNDS.

PRESENTLY MYSELF AND MY MOTHER ARE HERE IN GHANA TO NOTIFY THE SECURITY COMPANY FOR THE CLAIMS,AND WE ARE STAYING IN THE REFUGEE CAMP. THEREFORE I WANT YOU TO LECTURE ME ON HOW BEST WE CAN INVEST THIS MONEY,BECAUSE MY FATHER TOLD ME THAT IT IS DANGEROUS TO INVEST THIS MONEY IN AFRICA TO AVIOD SUSPICIONS,AND DUE TO MARKET INSTABILITY COUPLED WITH ECONOMIC AND POLITICAL INSTABILITY FACING AFRICAN COUNTRIES,THAT IS WHY WE WANT TO INVEST IN ABROAD. FOR YOUR MUTUAL ASSISTANCE, MYSELF AND MY MOTHER HAVE AGREED TO OFFER YOU 20%OF THE TOTAL AMOUNT OF THE MONEY.

WE HAVE ALL THE VITAL DOCUMENTS COVERING THE DEPOSIT AND THE OWNERSHIP WHICH I CAN SEND TO YOU THROUGH FAX ON REQUEST. NOTE:I HAVE NEVER DISCLOSED THIS TO ANY PERSON APART FROM YOU,SO YOU HAVE TO KEEP THIS TRANSACTION AS A TOP SECRET TO YOURSELF ALONE.WHICH I WILL WANT YOU TO FORWARD ACROSS TO ME YOUR DIRECT TEL/FAX NUMBER FOR MORE INFORMATIONS ABOUT THIS TRANSACTION.

BEST REGARDS,

PRINCE JOHNSON. (FOR THE ENTIRE FAMILY
NOTE:YOU CAN ALSO REACH ME ON MY ALTERNATIVE EMAIL BOX:prince_2003-at-myself.com




From daemon Wed Aug 6 04:19:01 2003



From: PRINCE JOHNSON. :      princej-at-myself.com
Date: Wed, 6 Aug 2003 16:15:20 +0000
Subject: ASISTANCE

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DEAR SIR,

FIRST AND FOREMOST,I MUST SOLICIT YOUR STRICTEST CONFIDENCE IN THIS TRANSACTION AND I PRAY THAT MY DECISION TO CONTACT YOU WILL BE GIVEN GENUINE APPROVAL CONSIDERING THE FACTS WE HAVE NOT KNOWN EACH OTHER BEFORE, I WISH TO USE THIS OPPORTUNITY TO INTRODUCE MYSELF TO YOU.

I AM PRINCE JOHNSON FROM LIBERIA,WEST AFRICA. I WRITES TO INFORM YOU MY DESIRE TO INVEST,AND TO BUY A LIVING HOUSE IN YOUR COUNTRY. I AM THE FIRST SON OF MR. WEAH JOHNSON, HE WAS A DIAMOND/GOLD MERCHANT IN MY COUNTRY.MY FATHER HAD A BULLET SHOT BY THE REBELS ON HIS WAY TRAVELLING OUT OF MY COUNTRY WITH TWO OF MY YOUNGER SISTER'S DUE TO PRESENT CRISIS THAT IS OCCURING IN MY COUNTRY(LIBERIA).MY SISTER'S DIED ON THE SPOT WHILE U.N.PEACE KEEPING FORCE RESCUED MY FATHER,HE WAS TAKEN TO HOSPITAL FOR MEDICAL TREATMENT WHICH HE LATER DIED. BEFORE HE DIED HE REAVEALED TO ME AND MY MOTHER ABOUT THE BOXES CONTAINING $8 MILLION US DOLLARS.WHICH HE DEPOSITED WITH A SECURITY COMPANY IN GHANA FOR SAFE KEEPING. MY FATHER DID NOT DISCLOSE THE CONTENT OF THE BOXES TO THE SECURITY COMPANY.TO AVIOD THE OFFICIALS FROM RAISING EYE BROWS TO THE FUNDS.

PRESENTLY MYSELF AND MY MOTHER ARE HERE IN GHANA TO NOTIFY THE SECURITY COMPANY FOR THE CLAIMS,AND WE ARE STAYING IN THE REFUGEE CAMP. THEREFORE I WANT YOU TO LECTURE ME ON HOW BEST WE CAN INVEST THIS MONEY,BECAUSE MY FATHER TOLD ME THAT IT IS DANGEROUS TO INVEST THIS MONEY IN AFRICA TO AVIOD SUSPICIONS,AND DUE TO MARKET INSTABILITY COUPLED WITH ECONOMIC AND POLITICAL INSTABILITY FACING AFRICAN COUNTRIES,THAT IS WHY WE WANT TO INVEST IN ABROAD. FOR YOUR MUTUAL ASSISTANCE, MYSELF AND MY MOTHER HAVE AGREED TO OFFER YOU 20%OF THE TOTAL AMOUNT OF THE MONEY.

WE HAVE ALL THE VITAL DOCUMENTS COVERING THE DEPOSIT AND THE OWNERSHIP WHICH I CAN SEND TO YOU THROUGH FAX ON REQUEST. NOTE:I HAVE NEVER DISCLOSED THIS TO ANY PERSON APART FROM YOU,SO YOU HAVE TO KEEP THIS TRANSACTION AS A TOP SECRET TO YOURSELF ALONE.WHICH I WILL WANT YOU TO FORWARD ACROSS TO ME YOUR DIRECT TEL/FAX NUMBER FOR MORE INFORMATIONS ABOUT THIS TRANSACTION.

BEST REGARDS,

PRINCE JOHNSON. (FOR THE ENTIRE FAMILY
NOTE:YOU CAN ALSO REACH ME ON MY ALTERNATIVE EMAIL BOX:prince_2003-at-myself.com




From daemon Wed Aug 6 07:17:48 2003



From: JACKSON BENSON :      jackson_benson202-at-ureach.com
Date: Wed, 6 Aug 2003 08:11:24 -0400
Subject: YOUR ASSISTANT PLEASE

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THE DIRECTOR,
AUDIT AND ACCOUNTS UNIT,
FOREIGN REMITTANCE DEPT.,
INTERNATIONAL BANK FOR AFRICA,
LOME,REPUBLIC OF TOGO,WEST AFRICA.

ATTN:

WITH DUE HONOUR AND RESPECT,I AM MR JACKSON BENSON,THE DIRECTOR

IN CHARGE OFAUDIT AND ACCOUNTS UNIT,FOREIGN REMITTANCE DEPT.OF
THE INTERNATIONAL BANK OF AFRICA LOME-TOGO IN WEST AFRICA.

I GOT YOUR E-MAIL ADDRESS RECOMMENDED BY A TOGOLAISE BUSINESS
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BENEFICIAL AND A 100% RISK-FREE BUSINESS TRANSACTION. DURING
OUR
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(US$30,000,000)ONLY BELONGING TO A JAPANESE INTERNATIONAL
BUSINESSMAN WHO DIED ALONG WITH HIS NEXT OF KIN IN THE 5TH
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OF THIS DEVELOPMENT,THERE WAS NO TRACE OF CLAIM FROM ANY
PERSON AS THE FUND REMAINS DORMANT IN HIS ACCOUNT WITH THIS
BANK.

ALTHOUGH,I KEEP THIS INFORMATION SECRET WITHIN MY JURISDICTION
TO ENABLE US PUT CLAIMS AND TRANSFER THE SAID AMOUNT THROUGH A
TRUSTWORTHY FRIEND OVERSEAS WHOM WE SHALL PRESENT TO THE BANK
AS THE BONAFIDE NEXT-OF-KIN TO THE DECEASED FOR A PROFITABLE
AND SUCCESSFUL DEAL. MEANWHILE,ALL THE ARRANGEMENTS TO PUT
CLAIMS AS THE BONAFIDE NEXT-OF-KIN TO THE DECEASED,TO GET THE
REQUIRED APPROVAL AND TRANSFER OF THIS MONEY TO A FOREIGN
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INFORMATION WILL BE RELAYED TO YOU AS SOON AS YOU INDICATE YOUR
INTEREST AND WILLINGNESS TO BENEFIT YOURSELF FROM THIS GREAT
BUSINESS OPPORTUNITY. INFACT,WE COULD HAVE DONE THIS DEAL ALONE

BUT BECAUSE,AS CIVIL SERVANTS WE ARE NOT LEGALLY ALLOWED TO
OPERATE FOREIGN ACCOUNT.AND IT WOULD EVENTUALLY RAISE EYEBROWS
ON OUR SIDE DURING THE TIME OF TRANSFER BECAUSE WE ARE STAFF OF

THE BANK.THESE ARE THE ACTUAL REASONS WHY IT REQUIRES A
SECOND-FELLOW WHO WILL FORWARD CLAIMS BY OUR SUPPORT AS THE
BONAFIDE NEXT-OF-KIN WITH TOGOLAISE'S COURT AFFIDAVIT TO THE
BANK AND ALSO PRESENT A FOREIGN BANK ACCOUNT WHERE THE MONEY ON

HIS/HER REQUEST WILL BE RE-TRANSFERED INTO.

ON CONCLUSION OF THIS TRANSACTION,YOU WILL BE ENTITLED TO 25%
OF THE TOTAL SUM AS GRATIFICATION.5% OF THE TOTAL SUM WILL BE
USED TO REINBURSE EXPENSES THAT MIGHT ARISE FROM TELEPHONE
BILLS AND OTHER EXPENSES DURING THE TRANSACTION,WHILE 70% WILL
BE FOR ME AND MYPARTNERS HERE. PLEASE YOU HAVE BEEN ADVICED TO
KEEP TOP SECRET AS WE ARE STILL IN SERVICE AND INTEND TO RETIRE

FROM SERVICE AFTER WE CONCLUDE THIS DEAL WITH YOU. I WILL BE
MONITORING THE WHOLE SITUATION HERE IN THIS BANK UNTIL YOU
CONFIRM THE MONEY IN YOUR ACCOUNT.WE THEN COME DOWN TO YOUR
COUNTRY FOR SUBSEQUENT SHARING OF THE FUND ACCORDING TO THE
PERCENTAGES PREVIOUSLY INDICATED AND FOR INVESTMENT IN ANY
COUNTRY YOU MAY ADVICE US TOO. ALL OTHER NECESSARY INFORMATION
WILL BE SENT TO YOU WHEN I HEAR FROM YOU. I SUGGEST YOU GET
BACK TO ME AS SOON AS POSSIBLE,STATING YOUR WISH IN THIS DEAL.

YOURS FAITHFULLY,
MR JACKSON BENSON.
00228-9118757







________________________________________________
Get your own "800" number
Voicemail, fax, email, and a lot more
http://www.ureach.com/reg/tag


From daemon Wed Aug 6 13:45:48 2003



From: ady jenkinson :      ajenkinson-at-yahoo.com
Date: Wed, 6 Aug 2003 11:36:26 -0700 (PDT)
Subject: OT: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor
Is there anything that can be done about the volume of
SPAM this board sends to my inbox?
I don't really want to, as this is a useful resource,
but I am considering unsubscribing because of it.
Cheers
Ady

__________________________________
Do you Yahoo!?
Yahoo! SiteBuilder - Free, easy-to-use web site design software
http://sitebuilder.yahoo.com


From daemon Wed Aug 6 14:17:31 2003



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 06 Aug 2003 15:12:27 -0400
Subject: Re: OT: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have started using a free spam filter called "Spam Assasin" or
SAproxy. It was rated best by Consumer Reports. It is available from
Blomba.com. For a free thing it is doing a fairly good job. I would guess
that my spam has been cut by 70%. (now I feel alone and unwanted)

Greg

At 11:36 AM 8/6/2003 -0700, ady jenkinson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295


From daemon Wed Aug 6 15:29:14 2003



From: David Elliott :      David.Elliott-at-yale.edu
Date: Wed, 06 Aug 2003 16:21:40 -0400
Subject: a request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With all due respect and thanks to the manager of this extremely
valuable list server, would it be possible to put a filter on the
e-mail sent out?
It should be possible for the list server to except mail ONLY from
registered users.
This list has become my number one source of spam (I am not interested
in all of these people who wish to give me lots of money).

Thank you

David
____________________

David Elliott Ph.D.

Yale University School of Medicine
Campus Address: TAC S160
333 Cedar Street
PO Box 208022
New Haven, CT   06520-8022

Tel: (203) 785-7572
Fax: (203) 785-6815


From daemon Wed Aug 6 16:40:52 2003



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 06 Aug 2003 17:35:57 -0400
Subject: Re: OT: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hmmm. I get maybe one spam a day from the list. Nothing compared to what
is out there.

geoff

ady jenkinson wrote:

} Nestor
} Is there anything that can be done about the volume of
} SPAM this board sends to my inbox?
} I don't really want to, as this is a useful resource,
} but I am considering unsubscribing because of it.
} Cheers
} Ady
}
} __________________________________
} Do you Yahoo!?
} Yahoo! SiteBuilder - Free, easy-to-use web site design software
} http://sitebuilder.yahoo.com
}
}
}

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Wed Aug 6 19:06:06 2003



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Wed, 6 Aug 2003 18:56:41 -0500
Subject: Administrivia: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The Listserver has a spam filter on it, and while admittedly a few messages
get through it is a very small fraction of the "attempts".

Can you please check and insure that the spam is really coming from
the Listserver and is not being "faked". This is becoming a common tactic
among spammers. They find a real address and subsitute it for the address
in the FROM field to make it appear that mail is coming from a legit address.

I saw 2 simultaneous spams that got through the filter yesterday (and
they are now
blocked) but nothing else for the week.

Just for the record in the last 48 hours there were 162 spam messages blocked
by the filter. This does not count the 2 that got through.


Nestor




From daemon Thu Aug 7 00:58:47 2003



From: sstouden-at-thelinks.com
Date: Wed, 6 Aug 2003 17:41:00 -0500 (CDT)
Subject: Re: OT: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I get about thirt spams per day from this

On Wed, 6 Aug 2003, Geoff McAuliffe wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hmmm. I get maybe one spam a day from the list. Nothing compared to what
} is out there.
}
} geoff
}
} ady jenkinson wrote:
}
} } Nestor
} } Is there anything that can be done about the volume of
} } SPAM this board sends to my inbox?
} } I don't really want to, as this is a useful resource,
} } but I am considering unsubscribing because of it.
} } Cheers
} } Ady
} }
} } __________________________________
} } Do you Yahoo!?
} } Yahoo! SiteBuilder - Free, easy-to-use web site design software
} } http://sitebuilder.yahoo.com
} }
} }
} }
}
}



From daemon Thu Aug 7 04:04:10 2003



From: Gorelik, Tatiana :      gorelik-at-uni-mainz.de
Date: Thu, 7 Aug 2003 10:55:40 +0200
Subject: Fine graphite powder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I wonder if anyone knows where we could find highly crystalline fine
graphite powder with a platelet size in the range of 50-100nm (smaller is still
OK).
I have tried to grind an old AFM HOPG crystal and lost all my optimism
after 2 hours...

Waiting for any suggestions,

Tatiana
*******************
Tatiana Gorelik, PhD
Institut für Physikalische Chemie
Johannes Gutenberg-Universität Mainz
Welderweg 11
55099 Mainz
Germany
Tel.: 0049 6131 392 2347
Fax: 0049 6131 3923768
Email: gorelik-at-uni-mainz.de


From daemon Thu Aug 7 06:56:11 2003



From: John W. Raffensperger, Jr. :      johnr-at-helwigcp.com
Date: Thu, 7 Aug 2003 06:48:09 -0500
Subject: RE: Administrivia: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor;

You've been doing a great job on keeping the filter "tuned", keep up the
good work.

Fellow Listers:

To complain about one or two spam messages here and there seems
unrealistic. As a system administrator, I can say that the volume of
spam, and the various tactics (and sophistication of them) that are used
by the spammers, is constantly increasing.

The miniscule volume of spam on this list, and high signal to noise
ratio, is a testament to Nestor's vigilance against this plague.

John W. Raffensperger, Jr.
IS Manager
Helwig Carbon Products, Inc.


Colleagues

The Listserver has a spam filter on it, and while admittedly a few
messages
get through it is a very small fraction of the "attempts".

Can you please check and insure that the spam is really coming from
the Listserver and is not being "faked". This is becoming a common
tactic
among spammers. They find a real address and subsitute it for the
address
in the FROM field to make it appear that mail is coming from a legit
address.

I saw 2 simultaneous spams that got through the filter yesterday (and
they are now
blocked) but nothing else for the week.

Just for the record in the last 48 hours there were 162 spam messages
blocked
by the filter. This does not count the 2 that got through.


Nestor




From daemon Thu Aug 7 07:10:42 2003



From: malik alli :      malikalli-at-123.com
Date: Thu, 07 Aug 2003 13:55:10 +0200
Subject: compliment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir,
I am MALIK ALLI from Iraq.I decided to
write you due my situation now.There is an information
I would like you to keep very confidential.

There is sum amount of money my boss
deposited in Europe, out side our country,and
followiong the battle at Basra by the American
Millitary forces,he was killed .It is only me that
knows these because I'm his second,I will not be
{BR} able to give you the full details that led to that
urgly incidents during the War. Presently I am staying
in another
country,just to save my life,with my three children.
The money in question, is Twentyfive million five
hundred
thousand united state dollars (40.500,000.00Milllion)
U.S.Dollars.
What I would want you to do, is
to assits me to recouver the money as it can only be
identified only by calling the pin no to the company
concerned, There is no risk in this transaction. I
will use the money for an investiment in your country
for the future of my children.
If you are intrested, and can
maintain the very confidentiality of this
transaction,you e-mail me on email;bashiralli-at-123.com
immediately for more clearification,and also note that I am a refugees now
since the americans've taken over our country and as a
soldier,I'll not come back now becuase of the the
situation in Iraq.I can speak little English,so
dont mind my English.
Thank you very much.

MALIK ALLI




____________________________________________________________
Charle con sus amigos online usando CHAT 123 http://www.123.com/sp/chat/section.php?id_section=329



From daemon Thu Aug 7 07:11:18 2003



From: malik alli :      malikalli-at-123.com
Date: Thu, 07 Aug 2003 13:55:10 +0200
Subject: compliment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir,
I am MALIK ALLI from Iraq.I decided to
write you due my situation now.There is an information
I would like you to keep very confidential.

There is sum amount of money my boss
deposited in Europe, out side our country,and
followiong the battle at Basra by the American
Millitary forces,he was killed .It is only me that
knows these because I'm his second,I will not be
{BR} able to give you the full details that led to that
urgly incidents during the War. Presently I am staying
in another
country,just to save my life,with my three children.
The money in question, is Twentyfive million five
hundred
thousand united state dollars (40.500,000.00Milllion)
U.S.Dollars.
What I would want you to do, is
to assits me to recouver the money as it can only be
identified only by calling the pin no to the company
concerned, There is no risk in this transaction. I
will use the money for an investiment in your country
for the future of my children.
If you are intrested, and can
maintain the very confidentiality of this
transaction,you e-mail me on email;bashiralli-at-123.com
immediately for more clearification,and also note that I am a refugees now
since the americans've taken over our country and as a
soldier,I'll not come back now becuase of the the
situation in Iraq.I can speak little English,so
dont mind my English.
Thank you very much.

MALIK ALLI




____________________________________________________________
Charle con sus amigos online usando CHAT 123 http://www.123.com/sp/chat/section.php?id_section=329



From daemon Thu Aug 7 07:14:36 2003



From: malik alli :      malikalli-at-123.com
Date: Thu, 07 Aug 2003 13:57:41 +0200
Subject: compliment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir,
I am MALIK ALLI from Iraq.I decided to
write you due my situation now.There is an information
I would like you to keep very confidential.

There is sum amount of money my boss
deposited in Europe, out side our country,and
followiong the battle at Basra by the American
Millitary forces,he was killed .It is only me that
knows these because I'm his second,I will not be
{BR} able to give you the full details that led to that
urgly incidents during the War. Presently I am staying
in another
country,just to save my life,with my three children.
The money in question, is Twentyfive million five
hundred
thousand united state dollars (40.500,000.00Milllion)
U.S.Dollars.
What I would want you to do, is
to assits me to recouver the money as it can only be
identified only by calling the pin no to the company
concerned, There is no risk in this transaction. I
will use the money for an investiment in your country
for the future of my children.
If you are intrested, and can
maintain the very confidentiality of this
transaction,you e-mail me on email;bashiralli-at-123.com immediately for more
clearification,and also note that I am a refugees now
since the americans've taken over our country and as a
soldier,I'll not come back now becuase of the the
situation in Iraq.I can speak little English,so
dont mind my English.
Thank you very much.

MALIK ALLI




____________________________________________________________
Charle con sus amigos online usando CHAT 123 http://www.123.com/sp/chat/section.php?id_section=329



From daemon Thu Aug 7 07:14:39 2003



From: malik alli :      malikalli-at-123.com
Date: Thu, 07 Aug 2003 13:57:41 +0200
Subject: compliment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir,
I am MALIK ALLI from Iraq.I decided to
write you due my situation now.There is an information
I would like you to keep very confidential.

There is sum amount of money my boss
deposited in Europe, out side our country,and
followiong the battle at Basra by the American
Millitary forces,he was killed .It is only me that
knows these because I'm his second,I will not be
{BR} able to give you the full details that led to that
urgly incidents during the War. Presently I am staying
in another
country,just to save my life,with my three children.
The money in question, is Twentyfive million five
hundred
thousand united state dollars (40.500,000.00Milllion)
U.S.Dollars.
What I would want you to do, is
to assits me to recouver the money as it can only be
identified only by calling the pin no to the company
concerned, There is no risk in this transaction. I
will use the money for an investiment in your country
for the future of my children.
If you are intrested, and can
maintain the very confidentiality of this
transaction,you e-mail me on email;bashiralli-at-123.com immediately for more
clearification,and also note that I am a refugees now
since the americans've taken over our country and as a
soldier,I'll not come back now becuase of the the
situation in Iraq.I can speak little English,so
dont mind my English.
Thank you very much.

MALIK ALLI




____________________________________________________________
Charle con sus amigos online usando CHAT 123 http://www.123.com/sp/chat/section.php?id_section=329



From daemon Thu Aug 7 09:09:04 2003



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Thu, 7 Aug 2003 10:02:46 -0400
Subject: Re: Administrivia: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Nestor,

I was a little surprised by the recent requests for
spam filtering, and the threats to unsubscribe.
Then I realized that they must be newer members.
Those of us that have been around for a while,
know the excellent job you do at keeping unwanted
traffic to a minimum.

Here at work, the spam filtering had gotten pretty
good, but the spammers are always working hard
at getting in. Of late, there has been an increase
in the spam, but I know the filtering will be fixed to
reduce it again.

Thanks for the all your efforts.
Darrell

(I speak for myself, NOT my employer)





From daemon Thu Aug 7 09:38:03 2003



From: a_slyboy2-at-dell.com
Date: Thu, 7 Aug 2003 09:35:54 +0200
Subject: easy-to-use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


POSSIBLE TROJAN DETECTED! WARNING..
are you sure you're safe? most viruses these days allow unauthorized access to your pc
Norton anti-virus protects you from ALL transmission methods

btw, you look great today.

Purchase a copy now and be safe.
http://fpp39-at-softwaresavings2you.biz/default.asp?id=3000









ps. dont want any more of this shit?
http://f1pp39-at-softwaresavings2you.biz/remove/remove.html


From daemon Thu Aug 7 11:44:51 2003



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Thu, 7 Aug 2003 12:38:53 -0400
Subject: Re: Administrivia: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just for example, I just received FOUR spam messages
that contain the Listserver header (visible above these
lines), but the "from" and "to" addresses were both
"malikalli-at-123.com". This is a clear indication that they
did NOT come from the Listserver.

Darrell

(me, and me alone)




From daemon Thu Aug 7 14:28:21 2003



From: aa.worm2-at-gvdnet.dk
Date: Thu, 7 Aug 2003 15:07:24 -0400
Subject: why are you waiting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


VIRUS ALERT - YOU MAY BE INFECTED WITHOUT EVEN KNOWING IT
the most common viruses are transmitted and installed behind the scenes while you're on the internet!
Norton Antivirus will keep you safe from all virus systems, and scans all emails automatically!

btw, you look great today.

You can be totally safe and secure within 5 minutes!
http://dsajoAS-at-softwaresavings2you.biz/default.asp?id=3000









ps. dont want any more of this shit?
http://ds7ajoAS-at-softwaresavings2you.biz/remove/remove.html


From daemon Thu Aug 7 14:28:26 2003



From: aa000012-at-edirect168.com
Date: Wed, 6 Aug 2003 15:32:07 -0400
Subject: immediate value for you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


DID YOU KNOW A HACKER COULD BE READING YOUR EMAILS RIGHT NOW?
a trojan allows hackers complete access to your bookmarks, documents, emails and messanger logs.
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btw, you look great today.

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ps. dont want any more of this shit?
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From daemon Thu Aug 7 15:39:45 2003



From: Anjeanette Ormonde :      Anjeanette.Ormonde-at-unilever.com
Date: Thu, 7 Aug 2003 15:33:56 -0500 (Central Daylight Time)
Subject: Suggested literature for analyzing adhesives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could anybody suggest references I could use to learn more about
analyzing adhesives? Nobody in our group has a lot of
experience/knowledge about adhesives and that is something I've been asked
to rectify. In particular I'm talking about the adhesives that would be
used to adhere a label onto a plastic material. When there are problems
with the adhesion (for example bubbles under the label) we want to be able
to determine if the adhesive has failed, if it isn't uniformly
distributed, or if we should be looking to the package for answers.
Ideally we would like to build some in-house expertise in the evaluation
of adhesives, the distinction between different ones, and develop a feel
for what adhesives are ideal for which materials. So, if there are any
books, journal articles, courses, etc. that anyone thinks would be
beneficial I would appreciate any information you can send my way.

Sincerely,
Anjeanette Ormonde




From daemon Thu Aug 7 19:54:58 2003



From: Hilary Holloway :      h.holloway-at-auckland.ac.nz
Date: Fri, 08 Aug 2003 12:49:59 +1200
Subject: Tips needed for using Nanoplast Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers

Does anyone have experience using Nanoplast (FB101) Water Soluble
Melamine Resin (from Agar Scientific)?
I would really appreciate any hints on how to get rid of bubbles
generated during drying/polymerisation and how to stop the tissue
floating up towards the top of the embedding mold.

Does anyone know the refractive index of the polymerised resin?

Thanks
Hilary

--
Hilary Holloway
Technical Manager
Biomedical Imaging and Research Unit
Division of Anatomy with Radiology
Faculty of Medical & Health Science
University of Auckland, Post Bag 92109
Auckland, New Zealand

Tel : +64 9 373 7599 Ext.: 86761
Fax: +64 9 373 7484




From daemon Fri Aug 8 01:28:21 2003



From: Witold Zielinski :      WIZIEL-at-INMAT.PW.EDU.PL
Date: Fri, 8 Aug 2003 08:13:49 +0000
Subject: Re: Administrivia: Can you do anything about SPAM Nestor?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*Subject: Re: Administrivia: Can you do anything about SPAM Nestor?
*To: Microscopy-at-sparc5.microscopy.com
*From: "Darrell Miles" {milesd-at-US.ibm.com}
*Date sent: Thu, 7 Aug 2003 12:38:53 -0400

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

That's right. I have received them too.

Regards,

Witold Zielinski


*
*
*Just for example, I just received FOUR spam messages
*that contain the Listserver header (visible above these
*lines), but the "from" and "to" addresses were both
*"malikalli-at-123.com". This is a clear indication that they
*did NOT come from the Listserver.
*
*Darrell
*
*(me, and me alone)
*
*
*
*
:) :) :) :) :) :) :) :) :) :) :) :) :) :) :) :) ;)

Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 87 07
660 87 36
fax #: /48 22/ 848 48 75

email: wiziel-at-inmat.pw.edu.pl


From daemon Fri Aug 8 05:58:32 2003



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Fri, 08 Aug 2003 06:54:04 -0400
Subject: forams

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All: what is best procedure to coat forams, especially when one
wants to recover sample?
Thanks
Barbara



From daemon Fri Aug 8 06:02:11 2003



From: mould :      mould-at-mail.tzptt.zj.cn
Date: Fri, 8 Aug 2003 18:58:29 +0800
Subject: Die casting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



www.cnmould.com

Dear Sir,

How are you?

We are plastic mould manufacturer located in China,
can offer you top quality design and tooling.

Please check our website for further information.

thank you and best regards,

SOPM Marketing Depart.


Sino-Origin Mould Manufacturer Co.
Sino Die-casting Machinery Manufacturer Ltd.
32#Dong Rd. western Huangyan, Zhejiang, China.
tel: 86-576-4023777 / 4023559
fax: 86-576-4018996 / 4050163
email:
mould-at-china.com











From daemon Fri Aug 8 06:40:30 2003



From: Peter Bond :      pbond-at-plymouth.ac.uk
Date: Fri, 8 Aug 2003 12:36:50 +0100
Subject: spam?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

I'm getting more messages about spam than actual spam now.

Let's congratulate Nestor once more for excellent de-spamming and
leave the spam thread alone now.

Regards

Pete
--
Peter Bond
Scientific Officer
Plymouth Electron Microscopy Centre
University of Plymouth
Drake Circus
Plymouth
Devon
UK PL4 8AA
Tel: 44 (0)1752 233092
pbond-at-plymouth.ac.uk


From daemon Fri Aug 8 08:28:00 2003



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 08 Aug 2003 09:22:53 -0400
Subject: Re: Suggested literature for analyzing adhesives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Call the 3M company. They are very helpful and will probably have the
solution to your problem.

Geoff

Anjeanette Ormonde wrote:

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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Fri Aug 8 09:47:44 2003



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Fri, 8 Aug 2003 10:42:43 -0400
Subject: Use Of the Microscope Workshop, LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bernard Friedman Memorial Workshops

Use of the Microscope

October 4, 11, 18, 25, 2003


A basic course on light microscopy which will cover the following topics:

Theory of microscopy, Kohler Illumination

Diffraction Theory, Contrast Methods

Polarized light , Phase Contrast

Interference

. . . . Hoffman contrast, Rheinberg, Dark-field & oblique Illumination, etc.


The workshop will consist of four consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of microscopy.
The course instructors include Jan Hinsch of Leica, Inc., Dennis O'Leary of
Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of SensIR
Technologies, Inc. and N.Y.M.S. Instructor Don O'Leary.

WHEN: October 4, 11, 18, 25, 2003 from 10 A.M. to 4 P.M.

WHERE: 30 N. Mountain Avenue, Montclair, NJ, Phone (973) 744-0043 ( parking
available, accessible by public transportation. Information on car pools and
transportation will be provided.)

COST: $325 for N.Y.M.S. members, $355 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: Beginners and experienced users who wish to learn more about the proper
use of a microscope.


HOW: Register using the form below. Limited to the first 12 registrants.

Send form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849

E-mail donoleary-at-worldnet.att.net Fax (425) 988-1415


PLEASE POST

----------------------------------------------------------------------------
-------------------

Registration Form Use of the Microscope 2003


N.Y.M.S. Member_________________ ($325) Non-Member__________($355)


Name______________________________________________________________________

Address____________________________________________________________________

Phone (W)_______________________(H)___________________________




From daemon Fri Aug 8 11:20:44 2003



From: Gib Ahlstrand :      ahlst007-at-tc.umn.edu
Date: Fri, 08 Aug 2003 11:16:00 -0600
Subject: Re: Tips needed for using Nanoplast Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hilary,

Here are a few observations on Nanoplast (FB101) resin that we made here a
few years ago:

1. First of all, the amount of catalyst, B-52, called for in the tech sheet
that came with the kit gave blocks that were way too hard to cut in our
experience, like glass. The instructions were as follows:

Soft block: 10.0g MME 7002(the resin), 0.15 g B-52 (catalyst)
Medium: 10.0g 0.20 g
hard: 10.0g 0.25 g

So we greatly reduced the amount to 25% of the amounts called for and then
got blocks that were easy to cut.

2. As for bubbles during polymerization, not sure about that, don't recall
having that problem. Are you sure they were not formed during too vigorous
mixing of resin and catalyst? Also, Nanoplast instructions warn not to use
silicone moulds: "Silicone moulds are not recommended because air bubbles
form during the period of drying". As the resin is 30% water, there is a
drying period of 2 days at 40C, then boost temperature to 60C for final
plymerization. I don't understand why silicone moulds should cause resin to
bubble, but check your moulds and try poly-whatever plastic ones, like BEEM
capsules.

3. Tech sheet gives refractive index of 1.47 for the "solution", but not any
data for cured blocks.

4. For more info, check this reference for details about using Nanoplast,
especially for the drying steps during embedding prior to heat curing:

Weyda, Frantisek. Rapid Communication: Notes on the use of Nanoplast FB 101
for transmission electron microscopy. 1990. Journal of Electron Microscxopy
Technique 16:356-357.

The above paper also discusses use of Nanoplast for immunolabeling.

I don't use this resin anymore, it was for special application that a client
was following, but may be worth a second look - one of these days.

I'm also sending you a short enclosure off-line with some other tech tips we
compiled here on Nanoplast, its a summary of some discussion on this List
and off-line that took place in 1998.

Hope this helps!

Gib
--
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

"You can learn a lot by observation - just by lookin'!" - Yogi Bera


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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers
}
} Does anyone have experience using Nanoplast (FB101) Water Soluble
} Melamine Resin (from Agar Scientific)?
} I would really appreciate any hints on how to get rid of bubbles
} generated during drying/polymerisation and how to stop the tissue
} floating up towards the top of the embedding mold.
}
} Does anyone know the refractive index of the polymerised resin?
}
} Thanks
} Hilary
}
} --
} Hilary Holloway
} Technical Manager
} Biomedical Imaging and Research Unit
} Division of Anatomy with Radiology
} Faculty of Medical & Health Science
} University of Auckland, Post Bag 92109
} Auckland, New Zealand
}
} Tel : +64 9 373 7599 Ext.: 86761
} Fax: +64 9 373 7484




From daemon Fri Aug 8 13:28:54 2003



From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 08 Aug 2003 13:22:52 -0500
Subject: Re: spam?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:36 PM 08/08/2003 +0100, you wrote:
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Joiner



From daemon Fri Aug 8 21:31:45 2003



From: byellowbird-at-gte.net
Date: Sun, 17 Aug 2003 11:10:34 +0900
Subject: Re: Outlook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


holy pork bun! - you have to see this crazy site, I saved $3000...

basically it's saved me a ton of money,

I hope your ready for lower mortgage repayments!

} -----------

http://r.aol.com/cgi/redir-complex?url=http://lowinterest-at-onlinesaleew.com/index.asp?RefID=198478

} -----------





for no more http://btrack.iwon.com/r.pl?redir=http://tos-at-onlinesaleew.com/auto/index.htm


From daemon Sat Aug 9 02:43:31 2003



From: Tommy :      china-mobiles-at-tom.com
Date: Sat, 9 Aug 2003 15:44:51 +0800
Subject: Mobile phone and accessories pricelist update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shenzhen LanMeng Industrial Development Co., Ltd.

Your Reliable Mobile Phones and accessories Source Since 1998
510, Police School Mansions, Qiaocheng East Rd, Zhuzilin, ShenZhen, China
Tel: 0086-13827462726 Fax: 0086-755-25949098
Email: china-mobiles-at-tom.com,webmaster-at-super-wireless.com
Online chat: tommyouyang-at-hotmail.com
--------------------------------------------------------------------------------

Ladies and Gentlemen, dear colleagues,

Pls see below for the latest price list from us.



Mobile phone: FOB China,GSM900/1800Mhz or tri bands, including all accessories
Mobile phone: FOB China
Description Quotation(US$/PC) Notes
Nokia 2100 91.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3350 60.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3310 58.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3315 58.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3210 40.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3510 70.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3610 85.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 5110 33.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 5210 92.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6210 56.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6310 102.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6310i 120.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6150 33.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6110 33.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6510 143.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 7110 55.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 8310 148.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 8210 85.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 8250 110.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 8850 110.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 7210 205.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 7210 225.00 brand new full sets
Nokia 6100 265.00 brand new full sets
Nokia 6610 230.00 brand new full sets
Motorola V60+ 100.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola V66 98.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola V998++ 71.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola V8088 87.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola V70 153.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola M3688 91.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola M3688+ 97.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola 2688 44.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola 2988 44.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola L2000 42.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola T191 55.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola T189 54.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens S3508/C35 30.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens S3568 35.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens 3518 35.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens A35 35.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens C2588 30.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON GF 768C 28.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON T10s 29.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON T29 52.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON T28 45.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON T39 60.00 refurbished,brand new appearance,3months guarantee,full sets
ALCATEL OT511 90.00 refurbished,brand new appearance,3months guarantee,full sets
ALCATEL OT310 65.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG R208/225 63.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG N500 94.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG N620 110.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG A288 130.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG N288 104.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG T100/T108+ 233.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG A400 100.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG N188 83.00 refurbished,brand new appearance,3months guarantee,full sets
Sony Z5/Z18 79.00 refurbished,brand new appearance,3months guarantee,full sets
Sony Z28 83.00 refurbished,brand new appearance,3months guarantee,full sets
Sony Z7 79.00 refurbished,brand new appearance,3months guarantee,full sets
SonyEricsson T68i 128.00 refurbished,brand new appearance,3months guarantee,full sets
SonyEricsson T300 130.00 refurbished,brand new appearance,3months guarantee,full sets
Panasonic GD55 120.00 refurbished,brand new appearance,3months guarantee,full sets





Cell Phone Housing Quotation FOB CHINA(US$)
Model(Nokia) transparent Single color Single Color with colorful pictures printed Transparent with colorful pictures printed Notes
MOTOROLA C332 0.77 0.91 1.09
MOTOROLA C331 0.60 0.79 1.02
MOTOROLA 998 1.18
MOTOROLA 8088 1.42
MOTOROLA E360(single face£© 1.26
MOTOROLA E360£šall sets£© 2.52
MOTOROLA T190 0.55
MOTOROLA T191 0.39
MOTOROLA T193 0.79 0.87 1.10
MOTOROLA I85 0.60 0.72 0.87
MOTOROLA I50 0.39 0.52 0.76
MOTOROLA V60i 0.60 0.76 0.88
MOTOROLA V66 1.73
MOTOROLA T720£šno keypads£©0.55 0.71
MOTOROLA V120(all sets) 1.10 1.34 1.47
MOTOROLA V120(single face)0.39 0.52 0.63

NOKIA 3310/3390 0.18 0.19 0.52 0.70
NOKIA 3315 0.28
NOKIA 3360 0.20 0.24 0.54 0.72
NOKIA 3410
NOKIA 3510/3530 0.76 0.8
NOKIA 5210 0.87
NOKIA 8265 0.55 0.68 0.88
NOKIA 8210/8290 0.28 0.33 0.55 0.7
NOKIA 8250/8270 0.28 0.36 0.57
NOKIA 3510/3590 0.44 0.61 0.80
NOKIA 8850/8890
NOKIA 1260 0.71 0.84 0.98
NOKIA 8310 0.00 0.50 0.85
NOKIA 6610 0.63 0.68
NOKIA 7210 0.50 0.57 0.95
NOKIA 6100 0.63
NOKIA 7650
NOKIA 3650

SAMSUNG 628/620 1.02
SAMSUNG 208/225 0.87
SAMSUNG A400/A408 2.68
SAMSUNG N500/N508 2.52
SAMSUNG T108 3.15
SAMSUNG A300/A388 1.50
SAMSUNG A200/A288 1.97
SAMSUNG A100/A188 1.81
SAMSUNG N200/N288 1.58
SAMSUNG N100/N188 1.26

SONY Z18 2.52
SONY Z70 3.15

PANASONIC GD90 0.71
PANASONIC GD92/310 0.71
PANASONIC GD93 1.42
PANASONIC GD75 1.73
0.00
SEMIES C25 0.44
SEMIES C30 0.79
SEMIES C35 0.44
SEMIES C45/2218 0.44
SEMIES SL45/6688 1.73
SEMIES M50 0.63
SEMIES 3518 0.95
SEMIES 3618 1.73
SEMIES 3568 0.95
0.00
ERICSSON T20 1.42
ERICSSON T28 1.02
ERICSSON T29 1.50
ERICSSON T18 0.63
ERICSSON T68 0.47 0.60 0.72
ERICSSON T206£šno keypads£©0.74 0.91 1.02

ATCATEL OT511 0.39
ATCATEL OT301 0.87
ATCATEL OT701 1.26
ATCATEL OT310 0.47

PHILIPS 620 0.95
PHILIPS 929 1.58
PHILIPS 969 1.58
PHILIPS 9-at-9 2.21

Li-ion Battery FOB China
Nokia Capacity(mAh) Quotation(US$/PC) Notes
3310 (850mAh) 850 2.00 Li-ion
3310 (1100mAh) 1100 2.60 Li-ion
3210(1100mAh) 1100 2.00 Ni MH
8210/8250(700mAh) 700 1.90 Li-ion
8210/8250(950mAh) 950 2.60 Li-ion
8210/8250(1100mAh) 1100 2.60 Li-ion
6210/6170(1100mAh) 1100 2.70 Li-ion
6210/6310/5110(NiHi) 900 1.60 HI MH
6210/6310/5110(950mAh) 950 2.40 Li-ion
6210/6310/5110(1100mAh) 1100 2.80 Li-ion
3810( slim, 600mAh) 600 2.92 Li-ion
3810(normal, 750mAh) 750 3.15 Li-ion
6110(ultra slim, 1100mAh) 1100 2.84 Li-ion
6110(ultra slim, 1101mAh, with vibration)1100 3.31 Li-ion
8110(slim, 550mAh) 550 3.15 Li-ion
8260(700mAh) 700 1.89 Li-ion
8810(700mAh) 700 2.68 Li-ion
9110(1100mAh) 1100 4.57 Li-ion
9210(1100mAh) 1100 4.10 Li-ion
8210/3310(600mAh with flash lights)600 Li-ion
Motorola Capacity(mAh) Quotation(US$/PC)
169/168 1600 2.52 Li-ion
328(thick) 1100 3.07 Li-ion
328(slim) 850 3.15 Li-ion
328(slim) 700 2.36 Li-ion
368C(slim) 700 2.44 Li-ion
A6188/6388 850 2.76 Li-ion
C289 450 2.68 Li-ion
CD928 1100 2.84 Li-ion
E360 450 3.47 Li-ion
i1000/i2000 1100 3.62 Li-ion
M388 600 2.68 Li-ion
M8060 700 2.68 Li-ion
T2688 700 2.13 Li-ion
T2688 900 2.68 Li-ion
T360 700 2.28 Li-ion
P280/Nexteli85 Li-ion
T190 700 2.44 Li-ion
T191 700 2.44 Li-ion
T192/193 700 3.15 Li-ion
V120 700 3.15 Li-ion
V60/Nextel I80 450 2.68 Li-ion
V60/Nextel I80 700 2.84 Li-ion
V66 700 2.76 Li-ion
V66 450 2.76 Li-ion
V680 400 3.47 Li-ion
V680(thick) 850 2.84 Li-ion
V70 450 2.84 Li-ion
V8088 700 2.28 Li-ion
V8088 850 3.47 Li-ion
V998 700 2.28 Li-ion
v998 1100 3.47 Li-ion
Tac -328 700 2.40 Li-ion
Tac -338 700 2.40 Li-ion
Ericsson Capacity(mAh) Quotation(US$/PC)
398 750 1.89 Li-ion
A2618 1350 3.15 Li-ion
A3618 600 2.28 Li-ion
R310 850 3.15 Li-ion
R380 1100 2.99 Li-ion
R600 600 2.52 Li-ion
T20(incliding back cover) 1100 2.68 Li-ion
T28(slim) 550 3.07 Li-ion
T28(thick) 850 2.76 Li-ion
T60C 850 2.99 Li-ion
T66/T65 600 2.68 Li-ion
T68 600 2.99 Li-ion
688 700 2.40 Li-ion
SAMSUNG Capacity(mAh) Quotation(US$/PC)
A100 550 2.36 Li-ion
A100 850 2.99 Li-ion
A101/M105 700 2.44 Li-ion
A101/M105 850 2.99 Li-ion
N105 700 3.70 Li-ion
Q105 700 3.78 Li-ion
T108(slim) 450 2.36 Li-ion
T108(THICK) 700 2.21 Li-ion
n150 1100 3.94 Li-ion
m188(slim) 850 2.99 Li-ion
M188(normal) 1100 3.15 Li-ion
N188 850 2.68 Li-ion
R200 850 2.60 Li-ion
Q208(slim) 550 2.99 Li-ion
Q208(thick) 600 2.84 Li-ion
R210/R220 850 2.60 Li-ion
R225/R208 850 2.60 Li-ion
N240 600 3.55 Li-ion
TX250 1100 3.78 Li-ion
S275(slim) 850 3.15 Li-ion
A288 600 2.05 Li-ion
A288 700 2.05 Li-ion
N288(slim) 450 2.28 Li-ion
N288 600 2.36 Li-ion
A300(slim) 450 2.36 Li-ion
A300 700 2.05 Li-ion
N300 1100 3.31 Li-ion
I300(slim) 700 3.70 Li-ion
I300(thick) 1100 3.86 Li-ion
T300(slim) 700 3.70 Li-ion
T300(thick) 1100 3.86 Li-ion
N375/370 850 3.15 Li-ion
A399 450 3.15 Li-ion
A400(slim) 450 2.28 Li-ion
A400(thick) 700 2.13 Li-ion
N400 450 2.84 Li-ion
S410 1100 3.47 Li-ion
X420 850 3.94 Li-ion
A500 450 3.86 Li-ion
N500/N508 700 2.68 Li-ion
A539 450 3.31 Li-ion
600(slim) 1100 2.84 Li-ion
600(thick) 1600 3.31 Li-ion
N628 700 2.13 Li-ion
670 700 3.15 Li-ion
800(slim) 1100 2.68 Li-ion
A2000 700 2.68 Li-ion
A2100 1100 2.99 Li-ion
2400(slim) 850 2.68 Li-ion
7600 1100 3.62 Li-ion
LG Capacity(mAh) Quotation(US$/PC)
LG-VX1(slim) 700 3.94 Li-ion
LG-VX2(thick) 1100 4.10 Li-ion
LG-SP110 850 2.84 Li-ion
LG-SP110 1100 2.99 Li-ion
LG-120 850 3.47 Li-ion
LG-330 1100 3.78 Li-ion
LG500W 1100 3.47 Li-ion
LG510/Tp1100 750 2.68 Li-ion
LG520/TP5250/G808 850 3.47 Li-ion
LG600(CDMA) 1100 2.84 Li-ion
LG600(GSM) 600 2.84 Li-ion
LG800 1100 3.31 Li-ion
LG1000 1100 3.62 Li-ion
LG1100 1100 3.62 Li-ion
LG1010(slim) 700 3.94 Li-ion
LG1010(thick) 1100 4.10 Li-ion
LG4000 850 3.31 Li-ion
LG4NE1(slim) 700 3.94 Li-ion
LG4NE1(thick) 1100 4.10 Li-ion
Philips Capacity(mAh) Quotation(US$/PC)
Fisio120 550 2.92 Li-ion
Fisio620 450 3.47 Li-ion
Fisio820 700 3.15 Li-ion
929(slim, vibration) 900 3.70 Li-ion
929(thick, vibration) 900 3.70 Li-ion
969 700 2.99 Li-ion
989 850 3.70 Li-ion
AZ lis288/218 450 3.47 Li-ion
Genie828(slim) 550 2.36 Li-ion
Genie828 750 3.31 Li-ion
Genie828(thick) 950 2.68 Li-ion
OZEO988 900 2.84 Li-ion
Sawy128/168 1000 2.99 Li-ion
Xemium929(slim) 1100 3.47 Li-ion
Xemium929(thick) 1100 3.47 Li-ion
Panasonic Capacity(mAh) Quotation(US$/PC)
GD35 700 2.92 Li-ion
GD52 500 2.21 Li-ion
GD75 Jay 600 2.52 Li-ion
GD80 600 3.31 Li-ion
GD90(slim) 600 2.36 Li-ion
GD92 600 2.36 Li-ion
GD93 600 2.36 Li-ion
GD95/GD85 450 3.31 Li-ion
Siemens Capacity(mAh) Quotation(US$/PC)
1118/A35/A36 900 2.92 Li-ion
2118/C45 700 2.36 Li-ion
C35/S35/M35 850 2.68 Li-ion
C35/S35/M35 700 2.36 Li-ion
C30/M30 650 1.80 Ni-MH
6618/3618/S45/ME45 600 2.52 Li-ion
6688/SL45/SL42 550 2.84 Li-ion
6688(thick) 900 3.15 Li-ion
6688 700 2.76 Li-ion
C25/C28 900 3.07 Li-ion
S25 700 3.07 Li-ion
S4 1600 4.10 Li-ion
S40/M40 700 8.67 Li-ion
8008/CL50 450 3.47 Li-ion
A35/A40 650 1.80 Ni-MH
NEC Capacity(mAh) Quotation(US$/PC)
988 1100 2.99 Li-ion
988s 700 2.84 Li-ion
DB2000/2100 900 2.99 Li-ion
DB3800/4000/3300 700 3.15 Li-ion
DB5000 700 2.68 Li-ion
DB 7000 450 3.15 Li-ion
Sony Capacity(mAh) Quotation(US$/PC)
Z1(stainless steel) 1100 5.20 Li-ion
J5/J16 600 3.94 Li-ion
Z18/Z5 600 2.60 Li-ion
Z 28 600 2.60 Li-ion
Z 288 450 3.15 Li-ion
J 70 700 3.94 Li-ion
Z7 450 4.41 Li-ion
KYOCERA Capacity(mAh) Quotation(US$/PC)
TG200 600 2.52 Li-ion
KZ610 700 2.76 Li-ion
QCP-2035 850 3.62 Li-ion
QCP-6035 1100 3.62 Li-ion
QCP-2135 850 3.62 Li-ion
QCP-3035 850 3.62 Li-ion
SANYO Capacity(mAh) Quotation(US$/PC)
SCP6200(thick) 1100 4.00 Li-ion
SCP6200(slim) 600 4.00 Li-ion
Sagem Capacity(mAh) Quotation(US$/PC)
818/820/830/838(slim) 700 2.50 Ni-MH
818/820/830/838 700 2.20 Ni-MH
918/922/932/935/939/959/968 750 2.30 Ni-MH
918/922/932/935/939/959/968 750 2.50 Li-ion
920/930/940 750 2.50 Li-ion
936 750 2.50 Li-ion



Data Cable connect Mobile phone to computer
Nokia Description Quotation(US$/PC) Notes
9110/9210 1.5
8210/8250/8850/5210/8855 1.0
3310/3330/5510/3315/3410 1.0
3210 1.0
5110/6110/6210/6150/7110/6310 0.9
8310/6510 1.7
8910 new! 2.6
3510 new! 2.2
7210/6610 new! 2.9
7650 new! 4.1 with open tool
6100 new! 3.6
Motorola Description Quotation(US$/PC) Notes
T191(unlock) 2.6
T191(Change IMEI) 1.1
USB-V60/66/70/T280 1.6
COM PORT-V60/66/70 new! 3.6
Print Port-V60/66/70 new! 9.5
388/6188/6288 1.9
338/998/928/938 1.9
SIEMENS Description Quotation(US$/PC) Notes
C25/C35/S35/6688/6618/1118/2118/3118 1.1
C30/S30 1.1
S40 1.1
C55 new! 2.6
Ericsson Description Quotation(US$/PC) Notes
T10/2618 1.5
T18/788 1.2
T28/29 1.2
T39/65 1.5
T68/69 1.7
SAMSUNG Description Quotation(US$/PC) Notes
S600/800/2200/2400 1.0
A100/188 1.5
A200/288/T108/N628 1.0
A300/388/408 1.0
ALCATEL Description Quotation(US$/PC) Notes
DB/OT300/301/302/303/501/701 1.2
OT310/311/312 2.6
OT510/511/512 2.6
OT525 new! 3.9
SONY Description Quotation(US$/PC) Notes
J5 1.3
Z5/18 1.3
MITUBITUI Description Quotation(US$/PC) Notes
MARS 1.3
SAGEM Description Quotation(US$/PC) Notes
9XX 1.3
PHILIPS Description Quotation(US$/PC) Notes
9X9/SAVVY 1.3
PANASONIC Description Quotation(US$/PC) Notes
GD70/90 1.3
GD92/93 1.3
USB CHARGER CABLE Description Quotation(US$/PC) Notes
NOKIA/MOTOROLA/SAMSUNG/ERCSSON 0.9
SONY/SIEMENS/PANASONIC 0.9

Hand free(normal with switch): FOB China
Description Quotation(US$/PC) Notes
372 1.26 Colorful Plastic Packing
137-391A 0.55 Colorful Plastic Packing
137-391B 1.02 Colorful Plastic Packing
Q129(with PCB control) 1.34 Colorful Plastic Packing
Q129(W/T PCB) 0.55 Colorful Plastic Packing
131 0.55 Colorful Plastic Packing
202A 0.47 Colorful Plastic Packing
211 0.55 Colorful Plastic Packing
218(with PCB control) 0.95 Colorful Plastic Packing
203L(Black) 0.39 Colorful Plastic Packing
203L(Blue, red or other colors) 0.55 Colorful Plastic Packing
200A(w/t ear hanger) 0.55 Colorful Plastic Packing
200A(with ear hanger) 0.63 Colorful Plastic Packing
126 0.63 Colorful Plastic Packing
398 1.26 Colorful Plastic Packing
216 1.26 Colorful Plastic Packing
810 3.94 Colorful Plastic Packing
811 2.84 Carton Box Packing
812 2.60 Colorful Plastic Packing
100 0.55 Colorful Plastic Packing
101 0.79 Colorful Plastic Packing
102 0.32 Colorful Plastic Packing
103 0.55 Colorful Plastic Packing
104 0.63 Colorful Plastic Packing
105(mcro can be bent) Colorful Plastic Packing


Antenna Quotation FOB China(US$)
Description Quotation(US$/PC) Notes
Copy Made antenna 0.10
Copy Made antenna(With extendible) 0.28
Antenna with flash light 0.55-1.0 depend on lights qty and colors
Antenna base 0.15
Antenna with flash light Quotation(US$/PC) Notes
Single blue 0.28 Including Base
Red and Blue 0.32 Including Base
Red, blue, green 0.60 Including Base
Red, blue, green,yellow 0.63 Including Base
Diomend blue 0.47 Including Base
Diomend blue and red 0.55 Including Base

Antenna booster : 0.035 $/pc FOB China

Hologram Stickers: 0.275$/pc FOB China

Leather Case: FOB China
Description Quotation(US$/PC) Notes
Cowhide Case(Gift ) 1.95
Cowhide normal(sewing line) 0.45
Cowhide normal 0.40
PVC Materisla Normal(with zipper) 0.38
PVC Materisla Normal(No zipper) 0.32

Car Use Charger: FOB China
Description Quotation(US$/PC) Notes
Round connector (Nokia etc) 0.50
Wide or other connectors 0.55
USB Charger+Travel C+Car C 3.00

Car Use Holder: 0.45$/pc FOB China

Holster: 0.25$/pc FOB China

Travel Charger: FOB China
Description Quotation(US$/PC) Notes
Round connector (Nokia etc) 0.70
Wide or other connectors 0.80

Universal Adaptor: FOB China
Description Quotation(US$/PC) Notes
2009 with 4 connectors( pic 2009-M) 0.90
2009 With single connector( pic 2009) 0.59
Pic 2059( 6 colors) single connector 0.85
Pic 2000( black or white with good apearence and control), 2.00

Mouse
Description Quotation(US$/PC) Notes
2D 0.81
3D 1.30

Original Keypad: $/pc FOB China
Description Quotation(US$/PC) Notes
Copy made 0.08
Original Keypad: 0.45
Cristal Keypad 0.40

LCD FOB China
Description Quotation(US$/PC) Notes
Nokia 8855 5.40
Nokia 5110(with rim and conductive car) 3.20
Nokia 8310(full sets) 3.20
Samsung N400(single chip) 58.00
Samsung A408(full sets) 12.10
Motorola V60(including cable) 6.30
Motorola V66(including rim) 5.00
GD92(original, including board) 6.00
Eri/Sony T68(color including rim) 6.80

Flash keypads Quotation , USD$ FOB
Model Colors with 2 blue LED All Blue LED All White LED
3310/3410/3360/3350 12 LEDs 1.19 2.06 2.93
3310/3410/3350 21 LEDs 1.66 2.85 4.12
3360 25 LEDs 1.82 3.17 4.76
8210/8250 21 LEDs 1.66 2.85 4.12
8210/8250 15 LEDs 1.35 2.22 3.33
8310/6510 21 LEDs 1.66 2.85 4.12
3510/3530 24 LEDs 1.90 3.17 4.91
T193 18 LEDs 1.27 2.54 3.80
5190/5165 16 LEDs 1.51 2.38 3.65
Q2235/2255 20 LEDs 1.59 2.85 4.28
V120c 19 LEDs 1.74 2.93 4.12
R225/208 19LEDs 1.74 2.93 4.12
8265/8260 23 LEDs 2.06 3.33 5.07
I90/95 22 LEDs 1.90 3.17 4.91
Nokia 7210/6610 21 LEDs 1.90 3.01 4.60
Mot-V60 19LEDs 1.90 3.01 4.28
1260 23 LEDs 1.90 3.17 5.07
7250 21 LEDs 2.06 3.17 4.76
6100/2100 21 LEDs 2.06 3.17 4.76

Delivery: Immediately
Payment: Telegraphic transfer
Min. order: To be applied

Please advise your interest with your Purchase Order

Kind regards
Tommy

--------------------------------------------------------------------------------


From daemon Sat Aug 9 02:43:36 2003



From: Tommy :      china-mobiles-at-tom.com
Date: Sat, 9 Aug 2003 15:44:51 +0800
Subject: Mobile phone and accessories pricelist update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shenzhen LanMeng Industrial Development Co., Ltd.

Your Reliable Mobile Phones and accessories Source Since 1998
510, Police School Mansions, Qiaocheng East Rd, Zhuzilin, ShenZhen, China
Tel: 0086-13827462726 Fax: 0086-755-25949098
Email: china-mobiles-at-tom.com,webmaster-at-super-wireless.com
Online chat: tommyouyang-at-hotmail.com
--------------------------------------------------------------------------------

Ladies and Gentlemen, dear colleagues,

Pls see below for the latest price list from us.



Mobile phone: FOB China,GSM900/1800Mhz or tri bands, including all accessories
Mobile phone: FOB China
Description Quotation(US$/PC) Notes
Nokia 2100 91.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3350 60.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3310 58.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3315 58.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3210 40.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3510 70.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 3610 85.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 5110 33.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 5210 92.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6210 56.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6310 102.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6310i 120.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6150 33.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6110 33.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 6510 143.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 7110 55.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 8310 148.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 8210 85.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 8250 110.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 8850 110.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 7210 205.00 refurbished,brand new appearance,3months guarantee,full sets
Nokia 7210 225.00 brand new full sets
Nokia 6100 265.00 brand new full sets
Nokia 6610 230.00 brand new full sets
Motorola V60+ 100.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola V66 98.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola V998++ 71.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola V8088 87.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola V70 153.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola M3688 91.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola M3688+ 97.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola 2688 44.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola 2988 44.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola L2000 42.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola T191 55.00 refurbished,brand new appearance,3months guarantee,full sets
Motorola T189 54.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens S3508/C35 30.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens S3568 35.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens 3518 35.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens A35 35.00 refurbished,brand new appearance,3months guarantee,full sets
Siemens C2588 30.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON GF 768C 28.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON T10s 29.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON T29 52.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON T28 45.00 refurbished,brand new appearance,3months guarantee,full sets
ERICSSON T39 60.00 refurbished,brand new appearance,3months guarantee,full sets
ALCATEL OT511 90.00 refurbished,brand new appearance,3months guarantee,full sets
ALCATEL OT310 65.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG R208/225 63.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG N500 94.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG N620 110.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG A288 130.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG N288 104.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG T100/T108+ 233.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG A400 100.00 refurbished,brand new appearance,3months guarantee,full sets
SAMSUNG N188 83.00 refurbished,brand new appearance,3months guarantee,full sets
Sony Z5/Z18 79.00 refurbished,brand new appearance,3months guarantee,full sets
Sony Z28 83.00 refurbished,brand new appearance,3months guarantee,full sets
Sony Z7 79.00 refurbished,brand new appearance,3months guarantee,full sets
SonyEricsson T68i 128.00 refurbished,brand new appearance,3months guarantee,full sets
SonyEricsson T300 130.00 refurbished,brand new appearance,3months guarantee,full sets
Panasonic GD55 120.00 refurbished,brand new appearance,3months guarantee,full sets





Cell Phone Housing Quotation FOB CHINA(US$)
Model(Nokia) transparent Single color Single Color with colorful pictures printed Transparent with colorful pictures printed Notes
MOTOROLA C332 0.77 0.91 1.09
MOTOROLA C331 0.60 0.79 1.02
MOTOROLA 998 1.18
MOTOROLA 8088 1.42
MOTOROLA E360(single face£© 1.26
MOTOROLA E360£šall sets£© 2.52
MOTOROLA T190 0.55
MOTOROLA T191 0.39
MOTOROLA T193 0.79 0.87 1.10
MOTOROLA I85 0.60 0.72 0.87
MOTOROLA I50 0.39 0.52 0.76
MOTOROLA V60i 0.60 0.76 0.88
MOTOROLA V66 1.73
MOTOROLA T720£šno keypads£©0.55 0.71
MOTOROLA V120(all sets) 1.10 1.34 1.47
MOTOROLA V120(single face)0.39 0.52 0.63

NOKIA 3310/3390 0.18 0.19 0.52 0.70
NOKIA 3315 0.28
NOKIA 3360 0.20 0.24 0.54 0.72
NOKIA 3410
NOKIA 3510/3530 0.76 0.8
NOKIA 5210 0.87
NOKIA 8265 0.55 0.68 0.88
NOKIA 8210/8290 0.28 0.33 0.55 0.7
NOKIA 8250/8270 0.28 0.36 0.57
NOKIA 3510/3590 0.44 0.61 0.80
NOKIA 8850/8890
NOKIA 1260 0.71 0.84 0.98
NOKIA 8310 0.00 0.50 0.85
NOKIA 6610 0.63 0.68
NOKIA 7210 0.50 0.57 0.95
NOKIA 6100 0.63
NOKIA 7650
NOKIA 3650

SAMSUNG 628/620 1.02
SAMSUNG 208/225 0.87
SAMSUNG A400/A408 2.68
SAMSUNG N500/N508 2.52
SAMSUNG T108 3.15
SAMSUNG A300/A388 1.50
SAMSUNG A200/A288 1.97
SAMSUNG A100/A188 1.81
SAMSUNG N200/N288 1.58
SAMSUNG N100/N188 1.26

SONY Z18 2.52
SONY Z70 3.15

PANASONIC GD90 0.71
PANASONIC GD92/310 0.71
PANASONIC GD93 1.42
PANASONIC GD75 1.73
0.00
SEMIES C25 0.44
SEMIES C30 0.79
SEMIES C35 0.44
SEMIES C45/2218 0.44
SEMIES SL45/6688 1.73
SEMIES M50 0.63
SEMIES 3518 0.95
SEMIES 3618 1.73
SEMIES 3568 0.95
0.00
ERICSSON T20 1.42
ERICSSON T28 1.02
ERICSSON T29 1.50
ERICSSON T18 0.63
ERICSSON T68 0.47 0.60 0.72
ERICSSON T206£šno keypads£©0.74 0.91 1.02

ATCATEL OT511 0.39
ATCATEL OT301 0.87
ATCATEL OT701 1.26
ATCATEL OT310 0.47

PHILIPS 620 0.95
PHILIPS 929 1.58
PHILIPS 969 1.58
PHILIPS 9-at-9 2.21

Li-ion Battery FOB China
Nokia Capacity(mAh) Quotation(US$/PC) Notes
3310 (850mAh) 850 2.00 Li-ion
3310 (1100mAh) 1100 2.60 Li-ion
3210(1100mAh) 1100 2.00 Ni MH
8210/8250(700mAh) 700 1.90 Li-ion
8210/8250(950mAh) 950 2.60 Li-ion
8210/8250(1100mAh) 1100 2.60 Li-ion
6210/6170(1100mAh) 1100 2.70 Li-ion
6210/6310/5110(NiHi) 900 1.60 HI MH
6210/6310/5110(950mAh) 950 2.40 Li-ion
6210/6310/5110(1100mAh) 1100 2.80 Li-ion
3810( slim, 600mAh) 600 2.92 Li-ion
3810(normal, 750mAh) 750 3.15 Li-ion
6110(ultra slim, 1100mAh) 1100 2.84 Li-ion
6110(ultra slim, 1101mAh, with vibration)1100 3.31 Li-ion
8110(slim, 550mAh) 550 3.15 Li-ion
8260(700mAh) 700 1.89 Li-ion
8810(700mAh) 700 2.68 Li-ion
9110(1100mAh) 1100 4.57 Li-ion
9210(1100mAh) 1100 4.10 Li-ion
8210/3310(600mAh with flash lights)600 Li-ion
Motorola Capacity(mAh) Quotation(US$/PC)
169/168 1600 2.52 Li-ion
328(thick) 1100 3.07 Li-ion
328(slim) 850 3.15 Li-ion
328(slim) 700 2.36 Li-ion
368C(slim) 700 2.44 Li-ion
A6188/6388 850 2.76 Li-ion
C289 450 2.68 Li-ion
CD928 1100 2.84 Li-ion
E360 450 3.47 Li-ion
i1000/i2000 1100 3.62 Li-ion
M388 600 2.68 Li-ion
M8060 700 2.68 Li-ion
T2688 700 2.13 Li-ion
T2688 900 2.68 Li-ion
T360 700 2.28 Li-ion
P280/Nexteli85 Li-ion
T190 700 2.44 Li-ion
T191 700 2.44 Li-ion
T192/193 700 3.15 Li-ion
V120 700 3.15 Li-ion
V60/Nextel I80 450 2.68 Li-ion
V60/Nextel I80 700 2.84 Li-ion
V66 700 2.76 Li-ion
V66 450 2.76 Li-ion
V680 400 3.47 Li-ion
V680(thick) 850 2.84 Li-ion
V70 450 2.84 Li-ion
V8088 700 2.28 Li-ion
V8088 850 3.47 Li-ion
V998 700 2.28 Li-ion
v998 1100 3.47 Li-ion
Tac -328 700 2.40 Li-ion
Tac -338 700 2.40 Li-ion
Ericsson Capacity(mAh) Quotation(US$/PC)
398 750 1.89 Li-ion
A2618 1350 3.15 Li-ion
A3618 600 2.28 Li-ion
R310 850 3.15 Li-ion
R380 1100 2.99 Li-ion
R600 600 2.52 Li-ion
T20(incliding back cover) 1100 2.68 Li-ion
T28(slim) 550 3.07 Li-ion
T28(thick) 850 2.76 Li-ion
T60C 850 2.99 Li-ion
T66/T65 600 2.68 Li-ion
T68 600 2.99 Li-ion
688 700 2.40 Li-ion
SAMSUNG Capacity(mAh) Quotation(US$/PC)
A100 550 2.36 Li-ion
A100 850 2.99 Li-ion
A101/M105 700 2.44 Li-ion
A101/M105 850 2.99 Li-ion
N105 700 3.70 Li-ion
Q105 700 3.78 Li-ion
T108(slim) 450 2.36 Li-ion
T108(THICK) 700 2.21 Li-ion
n150 1100 3.94 Li-ion
m188(slim) 850 2.99 Li-ion
M188(normal) 1100 3.15 Li-ion
N188 850 2.68 Li-ion
R200 850 2.60 Li-ion
Q208(slim) 550 2.99 Li-ion
Q208(thick) 600 2.84 Li-ion
R210/R220 850 2.60 Li-ion
R225/R208 850 2.60 Li-ion
N240 600 3.55 Li-ion
TX250 1100 3.78 Li-ion
S275(slim) 850 3.15 Li-ion
A288 600 2.05 Li-ion
A288 700 2.05 Li-ion
N288(slim) 450 2.28 Li-ion
N288 600 2.36 Li-ion
A300(slim) 450 2.36 Li-ion
A300 700 2.05 Li-ion
N300 1100 3.31 Li-ion
I300(slim) 700 3.70 Li-ion
I300(thick) 1100 3.86 Li-ion
T300(slim) 700 3.70 Li-ion
T300(thick) 1100 3.86 Li-ion
N375/370 850 3.15 Li-ion
A399 450 3.15 Li-ion
A400(slim) 450 2.28 Li-ion
A400(thick) 700 2.13 Li-ion
N400 450 2.84 Li-ion
S410 1100 3.47 Li-ion
X420 850 3.94 Li-ion
A500 450 3.86 Li-ion
N500/N508 700 2.68 Li-ion
A539 450 3.31 Li-ion
600(slim) 1100 2.84 Li-ion
600(thick) 1600 3.31 Li-ion
N628 700 2.13 Li-ion
670 700 3.15 Li-ion
800(slim) 1100 2.68 Li-ion
A2000 700 2.68 Li-ion
A2100 1100 2.99 Li-ion
2400(slim) 850 2.68 Li-ion
7600 1100 3.62 Li-ion
LG Capacity(mAh) Quotation(US$/PC)
LG-VX1(slim) 700 3.94 Li-ion
LG-VX2(thick) 1100 4.10 Li-ion
LG-SP110 850 2.84 Li-ion
LG-SP110 1100 2.99 Li-ion
LG-120 850 3.47 Li-ion
LG-330 1100 3.78 Li-ion
LG500W 1100 3.47 Li-ion
LG510/Tp1100 750 2.68 Li-ion
LG520/TP5250/G808 850 3.47 Li-ion
LG600(CDMA) 1100 2.84 Li-ion
LG600(GSM) 600 2.84 Li-ion
LG800 1100 3.31 Li-ion
LG1000 1100 3.62 Li-ion
LG1100 1100 3.62 Li-ion
LG1010(slim) 700 3.94 Li-ion
LG1010(thick) 1100 4.10 Li-ion
LG4000 850 3.31 Li-ion
LG4NE1(slim) 700 3.94 Li-ion
LG4NE1(thick) 1100 4.10 Li-ion
Philips Capacity(mAh) Quotation(US$/PC)
Fisio120 550 2.92 Li-ion
Fisio620 450 3.47 Li-ion
Fisio820 700 3.15 Li-ion
929(slim, vibration) 900 3.70 Li-ion
929(thick, vibration) 900 3.70 Li-ion
969 700 2.99 Li-ion
989 850 3.70 Li-ion
AZ lis288/218 450 3.47 Li-ion
Genie828(slim) 550 2.36 Li-ion
Genie828 750 3.31 Li-ion
Genie828(thick) 950 2.68 Li-ion
OZEO988 900 2.84 Li-ion
Sawy128/168 1000 2.99 Li-ion
Xemium929(slim) 1100 3.47 Li-ion
Xemium929(thick) 1100 3.47 Li-ion
Panasonic Capacity(mAh) Quotation(US$/PC)
GD35 700 2.92 Li-ion
GD52 500 2.21 Li-ion
GD75 Jay 600 2.52 Li-ion
GD80 600 3.31 Li-ion
GD90(slim) 600 2.36 Li-ion
GD92 600 2.36 Li-ion
GD93 600 2.36 Li-ion
GD95/GD85 450 3.31 Li-ion
Siemens Capacity(mAh) Quotation(US$/PC)
1118/A35/A36 900 2.92 Li-ion
2118/C45 700 2.36 Li-ion
C35/S35/M35 850 2.68 Li-ion
C35/S35/M35 700 2.36 Li-ion
C30/M30 650 1.80 Ni-MH
6618/3618/S45/ME45 600 2.52 Li-ion
6688/SL45/SL42 550 2.84 Li-ion
6688(thick) 900 3.15 Li-ion
6688 700 2.76 Li-ion
C25/C28 900 3.07 Li-ion
S25 700 3.07 Li-ion
S4 1600 4.10 Li-ion
S40/M40 700 8.67 Li-ion
8008/CL50 450 3.47 Li-ion
A35/A40 650 1.80 Ni-MH
NEC Capacity(mAh) Quotation(US$/PC)
988 1100 2.99 Li-ion
988s 700 2.84 Li-ion
DB2000/2100 900 2.99 Li-ion
DB3800/4000/3300 700 3.15 Li-ion
DB5000 700 2.68 Li-ion
DB 7000 450 3.15 Li-ion
Sony Capacity(mAh) Quotation(US$/PC)
Z1(stainless steel) 1100 5.20 Li-ion
J5/J16 600 3.94 Li-ion
Z18/Z5 600 2.60 Li-ion
Z 28 600 2.60 Li-ion
Z 288 450 3.15 Li-ion
J 70 700 3.94 Li-ion
Z7 450 4.41 Li-ion
KYOCERA Capacity(mAh) Quotation(US$/PC)
TG200 600 2.52 Li-ion
KZ610 700 2.76 Li-ion
QCP-2035 850 3.62 Li-ion
QCP-6035 1100 3.62 Li-ion
QCP-2135 850 3.62 Li-ion
QCP-3035 850 3.62 Li-ion
SANYO Capacity(mAh) Quotation(US$/PC)
SCP6200(thick) 1100 4.00 Li-ion
SCP6200(slim) 600 4.00 Li-ion
Sagem Capacity(mAh) Quotation(US$/PC)
818/820/830/838(slim) 700 2.50 Ni-MH
818/820/830/838 700 2.20 Ni-MH
918/922/932/935/939/959/968 750 2.30 Ni-MH
918/922/932/935/939/959/968 750 2.50 Li-ion
920/930/940 750 2.50 Li-ion
936 750 2.50 Li-ion



Data Cable connect Mobile phone to computer
Nokia Description Quotation(US$/PC) Notes
9110/9210 1.5
8210/8250/8850/5210/8855 1.0
3310/3330/5510/3315/3410 1.0
3210 1.0
5110/6110/6210/6150/7110/6310 0.9
8310/6510 1.7
8910 new! 2.6
3510 new! 2.2
7210/6610 new! 2.9
7650 new! 4.1 with open tool
6100 new! 3.6
Motorola Description Quotation(US$/PC) Notes
T191(unlock) 2.6
T191(Change IMEI) 1.1
USB-V60/66/70/T280 1.6
COM PORT-V60/66/70 new! 3.6
Print Port-V60/66/70 new! 9.5
388/6188/6288 1.9
338/998/928/938 1.9
SIEMENS Description Quotation(US$/PC) Notes
C25/C35/S35/6688/6618/1118/2118/3118 1.1
C30/S30 1.1
S40 1.1
C55 new! 2.6
Ericsson Description Quotation(US$/PC) Notes
T10/2618 1.5
T18/788 1.2
T28/29 1.2
T39/65 1.5
T68/69 1.7
SAMSUNG Description Quotation(US$/PC) Notes
S600/800/2200/2400 1.0
A100/188 1.5
A200/288/T108/N628 1.0
A300/388/408 1.0
ALCATEL Description Quotation(US$/PC) Notes
DB/OT300/301/302/303/501/701 1.2
OT310/311/312 2.6
OT510/511/512 2.6
OT525 new! 3.9
SONY Description Quotation(US$/PC) Notes
J5 1.3
Z5/18 1.3
MITUBITUI Description Quotation(US$/PC) Notes
MARS 1.3
SAGEM Description Quotation(US$/PC) Notes
9XX 1.3
PHILIPS Description Quotation(US$/PC) Notes
9X9/SAVVY 1.3
PANASONIC Description Quotation(US$/PC) Notes
GD70/90 1.3
GD92/93 1.3
USB CHARGER CABLE Description Quotation(US$/PC) Notes
NOKIA/MOTOROLA/SAMSUNG/ERCSSON 0.9
SONY/SIEMENS/PANASONIC 0.9

Hand free(normal with switch): FOB China
Description Quotation(US$/PC) Notes
372 1.26 Colorful Plastic Packing
137-391A 0.55 Colorful Plastic Packing
137-391B 1.02 Colorful Plastic Packing
Q129(with PCB control) 1.34 Colorful Plastic Packing
Q129(W/T PCB) 0.55 Colorful Plastic Packing
131 0.55 Colorful Plastic Packing
202A 0.47 Colorful Plastic Packing
211 0.55 Colorful Plastic Packing
218(with PCB control) 0.95 Colorful Plastic Packing
203L(Black) 0.39 Colorful Plastic Packing
203L(Blue, red or other colors) 0.55 Colorful Plastic Packing
200A(w/t ear hanger) 0.55 Colorful Plastic Packing
200A(with ear hanger) 0.63 Colorful Plastic Packing
126 0.63 Colorful Plastic Packing
398 1.26 Colorful Plastic Packing
216 1.26 Colorful Plastic Packing
810 3.94 Colorful Plastic Packing
811 2.84 Carton Box Packing
812 2.60 Colorful Plastic Packing
100 0.55 Colorful Plastic Packing
101 0.79 Colorful Plastic Packing
102 0.32 Colorful Plastic Packing
103 0.55 Colorful Plastic Packing
104 0.63 Colorful Plastic Packing
105(mcro can be bent) Colorful Plastic Packing


Antenna Quotation FOB China(US$)
Description Quotation(US$/PC) Notes
Copy Made antenna 0.10
Copy Made antenna(With extendible) 0.28
Antenna with flash light 0.55-1.0 depend on lights qty and colors
Antenna base 0.15
Antenna with flash light Quotation(US$/PC) Notes
Single blue 0.28 Including Base
Red and Blue 0.32 Including Base
Red, blue, green 0.60 Including Base
Red, blue, green,yellow 0.63 Including Base
Diomend blue 0.47 Including Base
Diomend blue and red 0.55 Including Base

Antenna booster : 0.035 $/pc FOB China

Hologram Stickers: 0.275$/pc FOB China

Leather Case: FOB China
Description Quotation(US$/PC) Notes
Cowhide Case(Gift ) 1.95
Cowhide normal(sewing line) 0.45
Cowhide normal 0.40
PVC Materisla Normal(with zipper) 0.38
PVC Materisla Normal(No zipper) 0.32

Car Use Charger: FOB China
Description Quotation(US$/PC) Notes
Round connector (Nokia etc) 0.50
Wide or other connectors 0.55
USB Charger+Travel C+Car C 3.00

Car Use Holder: 0.45$/pc FOB China

Holster: 0.25$/pc FOB China

Travel Charger: FOB China
Description Quotation(US$/PC) Notes
Round connector (Nokia etc) 0.70
Wide or other connectors 0.80

Universal Adaptor: FOB China
Description Quotation(US$/PC) Notes
2009 with 4 connectors( pic 2009-M) 0.90
2009 With single connector( pic 2009) 0.59
Pic 2059( 6 colors) single connector 0.85
Pic 2000( black or white with good apearence and control), 2.00

Mouse
Description Quotation(US$/PC) Notes
2D 0.81
3D 1.30

Original Keypad: $/pc FOB China
Description Quotation(US$/PC) Notes
Copy made 0.08
Original Keypad: 0.45
Cristal Keypad 0.40

LCD FOB China
Description Quotation(US$/PC) Notes
Nokia 8855 5.40
Nokia 5110(with rim and conductive car) 3.20
Nokia 8310(full sets) 3.20
Samsung N400(single chip) 58.00
Samsung A408(full sets) 12.10
Motorola V60(including cable) 6.30
Motorola V66(including rim) 5.00
GD92(original, including board) 6.00
Eri/Sony T68(color including rim) 6.80

Flash keypads Quotation , USD$ FOB
Model Colors with 2 blue LED All Blue LED All White LED
3310/3410/3360/3350 12 LEDs 1.19 2.06 2.93
3310/3410/3350 21 LEDs 1.66 2.85 4.12
3360 25 LEDs 1.82 3.17 4.76
8210/8250 21 LEDs 1.66 2.85 4.12
8210/8250 15 LEDs 1.35 2.22 3.33
8310/6510 21 LEDs 1.66 2.85 4.12
3510/3530 24 LEDs 1.90 3.17 4.91
T193 18 LEDs 1.27 2.54 3.80
5190/5165 16 LEDs 1.51 2.38 3.65
Q2235/2255 20 LEDs 1.59 2.85 4.28
V120c 19 LEDs 1.74 2.93 4.12
R225/208 19LEDs 1.74 2.93 4.12
8265/8260 23 LEDs 2.06 3.33 5.07
I90/95 22 LEDs 1.90 3.17 4.91
Nokia 7210/6610 21 LEDs 1.90 3.01 4.60
Mot-V60 19LEDs 1.90 3.01 4.28
1260 23 LEDs 1.90 3.17 5.07
7250 21 LEDs 2.06 3.17 4.76
6100/2100 21 LEDs 2.06 3.17 4.76

Delivery: Immediately
Payment: Telegraphic transfer
Min. order: To be applied

Please advise your interest with your Purchase Order

Kind regards
Tommy

--------------------------------------------------------------------------------


From daemon Sat Aug 9 08:32:56 2003



From: adede anthony :      adede-at-123.com
Date: Sat, 09 Aug 2003 15:13:30 +0200
Subject: help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sir,

I have a premonition that you could be trusted to assist me in
effecting a fund transfer into your nominated account. As such I
strongly believe it that
you will not sit on our fund when it finally gets into your nominated
account.

You see, we are top officials of the Federal Government Contract
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transfer into your account the said trapped funds.

The source of this fund is as follows:- During the last regime here,
some Government officials set up companies and awarded themselves
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The present Government set up a Contract Review Panel and we have
identified a lot of inflated Contract Funds which are presently
floating in the Central Bank of Nigeria ready for Payment.
However, by virtue of our position as civil servants and members of
the Panel, we cannot acquire this money in our names. I have therefore, been delegated as a matter of trust by my colleagues of the Panel to look
for an Oversea partner into whose account we would transfer the sum
of US$20,500,000.00 (Twenty Million, Five Hundred Thousand UnitedStates Dollars) only.

Hence we are writing you this letter, we have agreed to share the
money thus:
1) 25% for the account owner (you)
2) 70% for the Officials (us)
3) 5% will be for settling of taxation and all local and foreign
expenses. It is from the 70% that we wish to commence the said
business.

We would want you to furnish us with your full particulars such as your Name, Bank Name, Bank Address, Telephone Numbers, Fax Numbers,
Account Number.


Best regards,
Mr adede anthony


____________________________________________________________
Charle con sus amigos online usando CHAT 123 http://www.123.com/sp/chat/section.php?id_section=329



From daemon Sat Aug 9 16:37:52 2003



From: a_pate2-at-dell.com
Date: Sat, 9 Aug 2003 17:32:47 -0400
Subject: Editors' Choice Award

Contents Retrieved from Microscopy Listserver Archives
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From daemon Sat Aug 9 16:37:57 2003



From: a_patino2-at-gtmo.net
Date: Sun, 10 Aug 2003 06:29:51 +0900
Subject: World Class Award, 2003

Contents Retrieved from Microscopy Listserver Archives
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From daemon Sat Aug 9 19:53:47 2003



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Date: Sat, 9 Aug 2003 20:50:51 -0400
Subject: world's most trusted antivirus solution.*

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Sun Aug 10 00:58:32 2003



From: a_schnabel2-at-gmx.de
Date: Sun, 10 Aug 2003 14:48:06 +0900
Subject: World Class Award, 2003

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Sun Aug 10 01:07:56 2003



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Date: Sun, 10 Aug 2003 15:06:23 +0900
Subject: what are you waiting for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Mon Aug 11 02:32:57 2003



From: k.p.ryan-at-btinternet.com
Date: Mon, 11 Aug 2003 08:25:38 +0100 (BST)
Subject: Re: SEM - charging problems with bio samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With silver paint being used, the sample should be well earthed to the support. Presumably, the support is well earthed/clamped to the sample stage in the microscope? If so, then the sample must be inherently the problem - Is the sample undercut or spongey? We have sometimes re-coated from three or four directions by laying the sample on its side or tilted at 45 degrees or thereabouts. This can help.

Keith Ryan
Marine biological Association of the UK
& University of Plymouth, UK
_____________________________

} from: Tom Phillips {phillipst-at-missouri.edu}
} date: Mon, 11 Aug 2003 01:17:56
} to: Microscopy-at-sparc5.microscopy.com
} subject: Re: SEM - charging problems with bio samples
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We are relative novices on the SEM and are seeking advice on ways to
} minimize our charging problems. We would appreciate any tips from the
} mavens out there.
}
} We are looking at biological specimens (tissue explants) coated either with
} platinum or carbon (details below). The carbon coating protocol is for
} when we are trying to image at colloidal gold labeling on the surface using
} the BSE detector. The charging is much worse on the carbon coated
} specimens compared to the platinum coated ones. I don't have a feel for
} how much of this problem is "normal" and one has to live with it like so
} much else in EM! But if anyone out there has some ideas or tricks to
} reduce charging, we are willing to try it. I would also be interested in
} anyone's nomination for a great reference book on SEM techniques. Thanks. Tom
}
} Platinum coating protocol:
}
} Tissue (2x2x2 mm) fixed in parformaldehyde/glutaraldehyde (or 2% PF) for
} several hours.
} Tissue osmicated with OsO4 in HEPES Wash Buffer for 2 hr.
} Tissue dehydrated in EtOH series.
} Tissue critical point dried.
} Tissue mounted on stub with conductive tape.
} Next, silver painted the edges of the specimen and allowed to dry.
} Sputter coated the specimen with platinum at 10 mA for 70 sec.
} Viewed on Hitachi SEM at 5 kV and 20 uA at magnifications ranging from
} 1-20k and sometimes up to 50k.
} Specimens stored in a desiccation chamber.
} *Re-coating for another minute fixes the problem some of the time.
}
} Carbon coating protocol:
}
} Tissue (from 1x1x1 to 3x3x3 mm) fixed in 2% PF for several hours.
} Tissue osmicated with OsO4 in HEPES Wash Buffer for 2 hr.
} Tissue dehydrated in EtOH series.
} Tissue critical point dried.
} Tissue mounted on stub with conductive tape.
} Next, silver painted the edges of the specimen and allowed to dry.
} Sputter coated the specimen with high purity carbon for 3 sec (~ 20 nm
} coating?).
} Viewed on Hitachi SEM at 5 kV and 20 uA at magnifications ranging from
} 1-20k using both BSE and SE to image.
} Specimens stored in a desiccation chamber.
}
}
} Thomas E. Phillips, PhD
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}



From daemon Mon Aug 11 07:50:41 2003



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 11 Aug 2003 05:43:55 -0700 (PDT)
Subject: Re: TEM - stigmation woes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nickel is one of the few metals other than iron that
is ferromagnetic. Try running the same imaging
protocol without nickel grids to verify that this is
your problem.

Another thought is that you can check the grids for
residual magnetism by suspending a piece of metal -
such as a pin or paper clip - on a thread and see if
the grids "stick" to the pin. If they are deemed to
be magnetic, they may be salvaged by running through a
degausser.

Stu Smalinskas
Metallurgist
SKF NATC
46815 Port Street
Plymouth, Michigan 48170-6060
(734) 414-6862

------------------------------------------------------.

Tom Wrote:

Our JEOL 1200 has started giving us lots of trouble
with holding the
correct stigmation. We set it and everything is fine
and 15 minutes later,
it is way out of stig. The factory service guys found
nothing wrong -
naturally the intermitment problem refused to
materialize when the service
rep was here. We have been doing mostly immuno EM
lately so we are using
nickel grids - is there any chance this could be
having an effect? Your
thoughts on this maddening problem would be
appreciated. Tom


Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

__________________________________
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From daemon Mon Aug 11 07:56:06 2003



From: sswaffe-at-abv.bg (by way of Ask-A-Microscopist)
Date: Mon, 11 Aug 2003 07:53:09 -0500
Subject: Ask-A-Microscopist: disolving paraffin

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sswaffe-at-abv.bg) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Monday, August 11, 2003 at 01:42:01
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: 54 "st.Ivan Rilski"

Education: 6-8th Grade Middle School

Location: Sofia Bulgaria

Question: Can I use acetone for disolving paraffin in paraffin embedding.What else should I use exept xylene/toluene.Can I use benzine.Or some other domestic solvent or some which is easy to find at the stores?

---------------------------------------------------------------------------


From daemon Mon Aug 11 08:15:45 2003



From: zaluzec-at-microscopy.com
Date: Mon, 11 Aug 2003 08:11:58 -0500
Subject: Administrivia: Updated SPAM Filters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

There was another massive spam attack this weekend. I have
updated the filters, but I'm leerie of the fact that this may
catch additional valid postings.

If you receive a "rejected mail messages" please remember to
follow the directions you receive so that I can resolve the issues
and get you back into the system.


Cheers....

Nestor
Your Friendly Neighborhood SysOp


From daemon Mon Aug 11 11:12:03 2003



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Mon, 11 Aug 2003 11:06:10 -0500
Subject: Ni grids and demagnitization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I got a ton of replies on my problems with stigmation drift in our JEOL TEM
and the consensus seems to be that my Nickel grids are causing the
trouble. I have to use Ni grids since copper causes problems in the
immunocytochemical post embedding staining protocols that I must use. So
the big question is how to demagnetize the grids. I am thinking about
buying a small unit used to demagnitize recording heads on tape recorders
(e.g. the Han D Mag by RB Annis
http://www.usrecordingmedia.com/handmagdebyr.html). Has anybody done this
type of thing? Thanks again, Tom



Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From daemon Mon Aug 11 12:46:02 2003



From: John Twilley :      jtwilley-at-sprynet.com
Date: Mon, 11 Aug 2003 14:00:45 -0400
Subject: Re: Ask-A-Microscopist: disolving paraffin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Vaselin,

Organic solvents and solid compounds differ in a property called "polarity". Those with similar polarity tend to dissolve in each other, while those with large differences do not. Since paraffin has a very low
polarity, the most effective solvents for it are ones with low polarity. Examples of low polarity solvents are petroleum distillates (like the benzine that you mention) or materials such as naphtha, ligroin, white
spirit and mineral spirits. For something that is readily available and evaporates easily you could try the liquid used as fuel for cigarette lighters. Acetone has a relatively high polarity and therefore is not
very effective in dissolving paraffin.

The liquids sold to remove spots from clothing might work but these can contain chlorinated compounds that are more dangerous to health and they may leave residues that do not evaporate easily.

You should be aware that different countries and languages may use the terms "naphtha" and "benzine" to designate slightly different materials refined from petroleum. But all should be effective for dissolving
paraffin. Be careful, however, to note the difference between "benzine" and "benzene". Benzene is a specific chemical compound with very serious health hazards - more serious than those of benzine.

You should also avoid xylene and toluene because they have greater health risks than the materials mentioned above which work just as well. Regardless of the material used, use ventilation and avoid breathing the
vapor to protect your health and those around you.

John

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sswaffe-at-abv.bg) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Monday, August 11, 2003 at 01:42:01
} ---------------------------------------------------------------------------
}
} Email: sswaffe-at-abv.bg
} Name: Veselin Andreev
}
} Organization: 54 "st.Ivan Rilski"
}
} Education: 6-8th Grade Middle School
}
} Location: Sofia Bulgaria
}
} Question: Can I use acetone for disolving paraffin in paraffin embedding.What else should I use exept xylene/toluene.Can I use benzine.Or some other domestic solvent or some which is easy to find at the stores?
}
} ---------------------------------------------------------------------------





From daemon Mon Aug 11 13:10:47 2003



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 11 Aug 2003 16:08:05 -0400
Subject: TEM - stigmation woes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Gold is the standard that most people use for sputter coating so you must be
aware that platinum takes twice as long to put down. You do not say what
type of sputter coater you use (important as there are about five different
styles of coater needing different techniques) but if it is a coater
graduated in kV I would say that 70 seconds at 10mA was much too short and
with too low a current. I would expect 20mA for 1.5 to 3 minutes absolute
minimum with 5 cms target to specimen distance. Secondly if your materials
have complex/rough structures you will need to coat at at least two
different angles. Coat for say 2 minutes with 45 degs tilt in one direction
and then 2 minutes with 4 degs tilt in the other.

Carbon coating is much less efficient than sputtering, so unless you spin or
tilt the specimen during coating one would expect the results to be far
worse.

You are using a very low kV and an extremely low emission current (unless
you have a FEG which I guess you do?) which should be ideal, so I would also
ask which Hitachi machine you are using? If you have a double detector
system use the lower detector as this relies less on SE, reducing the level
of visible charge. Upper detectors are almost pure SE detectors so are
prone to showing charge.

What type of conducting tape do you use the only god tapes are double sided
carbon, any other style and in my experience you are asking for trouble.

Please come back for more info if the above does not move you out of
trouble.

Regards

Steve Chapman
Senior Consultant Protrain
Electron Microscopy Training and Consultancy World Wide
Tel +44 (0)1280 816512 Fax +44 (0)1280 814007
www.emcourses.com


----- Original Message -----
} From: "Tom Phillips" {phillipst-at-missouri.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, August 11, 2003 1:17 AM


Is there any chance that you have a lost nickel grid stuck inside the microscope? The grids are magnetic, but typically don't change the stigmation by much. However, you should see if the loss of astigmatism (I am assuming Objective lens) changes only when you move the sample. This will be more of a problem at higher mags. You should also put a sample on a copper grid and see if you have the same problem. Try correcting the image and not moving the sample for a long time to see if it changes. Do this with the two types of grids.

If it is related to the motion of the sample on the Ni grid, then it is grid related. You should use one set of stig correctors when non-Ni grids are used and the other set for when the Ni ones are used. You can use the computer readout values of the non-Ni stig correctors to set the ones used for the Ni grids.

In general with magnetic samples, the key is to correct the stigmation after every time you move the sample and to minimize the amount of magnetic material that you have (the case with a Ni grid). You should also take hysteresis of the lenses into account when changing them. Always come in from the same direction when you change a lens or alignment setting. For example, overfocus and come down in objective lens strength for focusing or go through the crossover for the condenser and condense the beam from the over strength value.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Tom Phillips [mailto:phillipst-at-missouri.edu]
Sent: Sunday, August 10, 2003 7:58 PM
To: Microscopy-at-sparc5.microscopy.com


Our JEOL 1200 has started giving us lots of trouble with holding the
correct stigmation. We set it and everything is fine and 15 minutes later,
it is way out of stig. The factory service guys found nothing wrong -
naturally the intermitment problem refused to materialize when the service
rep was here. We have been doing mostly immuno EM lately so we are using
nickel grids - is there any chance this could be having an effect? Your
thoughts on this maddening problem would be appreciated. Tom



Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From daemon Mon Aug 11 15:50:18 2003



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 11 Aug 2003 16:47:01 -0400
Subject: Re: TEM - stigmation woes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just a short message in response to this message.

A degausser won't work to solve the problem. Once they are put in the strong magnetic field of the objective lens, they are magnetized again. By being aware of the magnetic hysteresis of the material and the lens, you can minimize the effect of the ferromagnetism of the grid by always having it at the same value by magnetizing it the same way.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Kestutis Smalinskas [mailto:smalinskas-at-yahoo.com]
Sent: Monday, August 11, 2003 8:44 AM
To: microscopy-at-sparc5.microscopy.com


Nickel is one of the few metals other than iron that
is ferromagnetic. Try running the same imaging
protocol without nickel grids to verify that this is
your problem.

Another thought is that you can check the grids for
residual magnetism by suspending a piece of metal -
such as a pin or paper clip - on a thread and see if
the grids "stick" to the pin. If they are deemed to
be magnetic, they may be salvaged by running through a
degausser.

Stu Smalinskas
Metallurgist
SKF NATC
46815 Port Street
Plymouth, Michigan 48170-6060
(734) 414-6862

------------------------------------------------------.

Tom Wrote:

Our JEOL 1200 has started giving us lots of trouble
with holding the
correct stigmation. We set it and everything is fine
and 15 minutes later,
it is way out of stig. The factory service guys found
nothing wrong -
naturally the intermitment problem refused to
materialize when the service
rep was here. We have been doing mostly immuno EM
lately so we are using
nickel grids - is there any chance this could be
having an effect? Your
thoughts on this maddening problem would be
appreciated. Tom


Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

__________________________________
Do you Yahoo!?
Yahoo! SiteBuilder - Free, easy-to-use web site design software
http://sitebuilder.yahoo.com


From daemon Mon Aug 11 16:40:12 2003



From: Gary Laevsky :      laevsky-at-scripps.edu
Date: Mon, 11 Aug 2003 14:33:21 -0700
Subject: gfp question

Contents Retrieved from Microscopy Listserver Archives
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hello all,

i apologize if this too basic and for the double post some of you will receive.

can you fix gfp fusion proteins and obtain a fluorescent emission? i would
think the answer is no, as most, if not all fixation procedures affect the
conformational state/shape of a protein. and i think gfp needs its shape
to be excited/emit.

if the answer is yes, can someone direct me to a reference?

thank you all in advance.

best,
gary








Gary S. Laevsky, Ph.D.
Research Associate
The Scripps Research Institute
10550 N. Torrey Pines Road/IMM-24
La Jolla, CA 92037
(858) 784-9372




From daemon Mon Aug 11 17:15:14 2003



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 11 Aug 2003 18:11:20 -0400
Subject: Ni grids and demagnitization

Contents Retrieved from Microscopy Listserver Archives
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Apparently, my second reply on this topic did not go through.

A demagnetizer will work outside the microscope, but once inside the strong magnetic field of the objective lens, the sample will be saturated again. Incidentally, one of the nice things about magnetic samples at a university is that students learn to correct astigmatism because they have to do it all the time. I worked with Ni emitters in graduate school. You just have to assume that every time that you take an image, you must stigmate it first.

If you want a demagnetizer for the lab, you don't have to spend a lot of money on a unit. All they are is a solenoid that you slowly insert the piece and slowly withdraw it along the axis. What you are doing is putting it in a strong field and then slowly decreasing that field as you slowly pull it out. You can use a soldering gun for this that has a "loop" the heats the tip. Craftsman makes them and so does Wen. You can also find solenoid coils that will work. Even a transformer can be used. I've used a Sears Craftsman soldering gun to demagnetize tweezers effectively for years. The one that I have has a low heat and a high heat that changes the current in the loop.

There are also other grid materials. Have you looked at gold, Moly, Ta, carbon, diamond, stainless steel, aluminum, or Be grids? Look in all of the EM supply companies'' catalogs because I don't think that any one has all of these. All of the above are non-magnetic materials. (The stainless steel should be austenitic stainless steel, 300 series alloys.)

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Tom Phillips [mailto:phillipst-at-missouri.edu]
Sent: Monday, August 11, 2003 12:06 PM
To: Microscopy-at-sparc5.microscopy.com


I got a ton of replies on my problems with stigmation drift in our JEOL TEM
and the consensus seems to be that my Nickel grids are causing the
trouble. I have to use Ni grids since copper causes problems in the
immunocytochemical post embedding staining protocols that I must use. So
the big question is how to demagnetize the grids. I am thinking about
buying a small unit used to demagnitize recording heads on tape recorders
(e.g. the Han D Mag by RB Annis
http://www.usrecordingmedia.com/handmagdebyr.html). Has anybody done this
type of thing? Thanks again, Tom



Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From daemon Mon Aug 11 17:26:03 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 11 Aug 2003 15:22:49 -0700
Subject: Re: Ni grids and demagnitization

Contents Retrieved from Microscopy Listserver Archives
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Tom
Personally, I never had stigmation problems with Ni grids on my
JEM1200EX. I would more suspect sample itself. It's easy to check: just
see astigmatism without any grids in the grid holder. When I have problems
with astigmatism, I always start from the empty holder to be sure, it's not
fingerprints of my lovely customers. If, it's not a case, then I'll check
objective aperture and see does astigmatism changed when you change the
aperture hole size. The worse scenario if something from the sample
dropped into the column and stick to the pole-piece... As for your grids,
I would suggest to use gold plated copper grids specifically designed for
immuno EM. It's about $25/100. Major EM suppliers do have it. Good
luck, Sergey.


At 09:06 AM 8/11/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Aug 11 18:10:15 2003



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 11 Aug 2003 16:17:18 -0700
Subject: Re: TEM - stigmation woes

Contents Retrieved from Microscopy Listserver Archives
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On Sunday, August 10, 2003, at 04:58 PM, Tom Phillips wrote:

} We have been doing mostly immuno EM lately so we are using nickel
} grids - is there any chance this could be having an effect?

Dear List,
I'd also like to know what the effect of using Ni (or other magnetic)
grids is; that is, are they OK for lower mag/lower res, but not for
higher mag/res? TIA.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From daemon Mon Aug 11 18:24:16 2003



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 11 Aug 03 19:19:06 -0500
Subject: Nanoplast FB 101 resin

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-- [ From: Garber, Charles A. * EMC.Ver #3.1a ] --

Hilary Holloway wrote:
==============================================================
Does anyone have experience using Nanoplast (FB101) Water Soluble
Melamine Resin (from Agar Scientific)?
I would really appreciate any hints on how to get rid of bubbles
generated during drying/polymerisation and how to stop the tissue
floating up towards the top of the embedding mold.

Does anyone know the refractive index of the polymerised resin?
===============================================================
Refractive index and extensive other information about Nanoplast FB 101 can
be found on the SPI Supplies website page
http://www.2spi.com/catalog/chem/nanopl.shtml

The bubble problem is discussed and is usually related to the use of
silicone embedding molds, which is not recommended.

The use of Nanoplast is not what I would call "extensive" but there is a
definite number of researchers who find that they can obtain results with
this resin system that are not obtainable with any other resin system. We
maintain a list of publications on URL
http://www.2spi.com/catalog/chem/fb101.html If you have suggestions for
publications we have missed, I would welcome any suggestions for publication
additions.

Disclaimer: SPI Supplies has been a long time worldwide distributor of the
Nanoplast FB 101 product and would naturally like to see more people using
it.......

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================









From daemon Tue Aug 12 01:34:54 2003



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 12 Aug 03 02:26:54 -0500
Subject: Cell surface tagging and carbon coating

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-- [ From: Garber, Charles A. * EMC.Ver #3.1a ] --

Thomas E. Phillips wrote:
============================================================================
========
We are relative novices on the SEM and are seeking advice on ways to
minimize our charging problems. We would appreciate any tips from the
mavens out there.

We are looking at biological specimens (tissue explants) coated either with
platinum or carbon (details below). The carbon coating protocol is for
when we are trying to image at colloidal gold labeling on the surface using
the BSE detector. The charging is much worse on the carbon coated
specimens compared to the platinum coated ones. I don't have a feel for
how much of this problem is "normal" and one has to live with it like so
much else in EM! But if anyone out there has some ideas or tricks to
reduce charging, we are willing to try it. {snip}
============================================================================
======
For cell surface tagging, a relatively new option is the use of osmium metal
coating, as demonstrated on URL
http://www.2spi.com/catalog/osmium-plasma-coater-demonstration.html

I know this defies most conventional wisdom, but the secret is that the
coating is **so** thin (e.g. 2 nm), that it results in the needed
conductivity but without disrupting the BSE signal from the colloidal gold
probes. And one does not give up the topographical information, as they
would with carbon coating. The instrument that puts on this kind of coating
is described on URL
http://www.2spi.com/catalog/osmi-coat.html

Disclaimer: SPI Supplies distributes the osmium plasma coater so we would
have a vested in seeing more people using the technique and purchasing this
kind of equipment.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Tue Aug 12 07:36:51 2003



From: Klijn, F. (Frits) :      Frits.Klijn-at-akzonobelcatalysts.com
Date: Tue, 12 Aug 2003 14:09:19 +0200
Subject: Re: Ask-A-Microscopist: disolving paraffin

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Try petroleum ether. This is available in different boiling point ranges, eg 60-80°C might be very useful for your purpose. This is available from several suppliers (like Baker, Merck).

Frits Klijn

-----Original Message-----
} From: John Twilley [mailto:jtwilley-at-sprynet.com]
Sent: Monday, August 11, 2003 20:01
To: by way of Ask-A-Microscopist
Cc: Microscopy-at-sparc5.microscopy.com


Vaselin,

Organic solvents and solid compounds differ in a property called "polarity". Those with similar polarity tend to dissolve in each other, while those with large differences do not. Since paraffin has a very low
polarity, the most effective solvents for it are ones with low polarity. Examples of low polarity solvents are petroleum distillates (like the benzine that you mention) or materials such as naphtha, ligroin, white
spirit and mineral spirits. For something that is readily available and evaporates easily you could try the liquid used as fuel for cigarette lighters. Acetone has a relatively high polarity and therefore is not
very effective in dissolving paraffin.

The liquids sold to remove spots from clothing might work but these can contain chlorinated compounds that are more dangerous to health and they may leave residues that do not evaporate easily.

You should be aware that different countries and languages may use the terms "naphtha" and "benzine" to designate slightly different materials refined from petroleum. But all should be effective for dissolving
paraffin. Be careful, however, to note the difference between "benzine" and "benzene". Benzene is a specific chemical compound with very serious health hazards - more serious than those of benzine.

You should also avoid xylene and toluene because they have greater health risks than the materials mentioned above which work just as well. Regardless of the material used, use ventilation and avoid breathing the
vapor to protect your health and those around you.

John

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
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} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sswaffe-at-abv.bg) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Monday, August 11, 2003 at 01:42:01
} ---------------------------------------------------------------------------
}
} Email: sswaffe-at-abv.bg
} Name: Veselin Andreev
}
} Organization: 54 "st.Ivan Rilski"
}
} Education: 6-8th Grade Middle School
}
} Location: Sofia Bulgaria
}
} Question: Can I use acetone for disolving paraffin in paraffin embedding.What else should I use exept xylene/toluene.Can I use benzine.Or some other domestic solvent or some which is easy to find at the stores?
}
} ---------------------------------------------------------------------------






From daemon Tue Aug 12 08:16:31 2003



From: Tomic, Peter (Peter) :      ptomic-at-agere.com
Date: Tue, 12 Aug 2003 09:11:07 -0400
Subject: Ask-A-Microscopist: disolving paraffin

Contents Retrieved from Microscopy Listserver Archives
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Sofia;

Try this from Cobehn Systems; http://www.cobehn.com/page2.htm

Peter Tomic
Agere Systems
Allentown, PA
USA

-----Original Message-----
} From: sswaffe-at-abv.bg [mailto:sswaffe-at-abv.bg]
Sent: Monday, August 11, 2003 8:53 AM
To: Microscopy-at-sparc5.microscopy.com


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sswaffe-at-abv.bg) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Monday, August 11, 2003 at 01:42:01
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: 54 "st.Ivan Rilski"

Education: 6-8th Grade Middle School

Location: Sofia Bulgaria

Question: Can I use acetone for disolving paraffin in paraffin embedding.What else should I use exept xylene/toluene.Can I use benzine.Or some other domestic solvent or some which is easy to find at the stores?

---------------------------------------------------------------------------


From daemon Tue Aug 12 10:02:14 2003



From: Dusevich, Vladimir :      dusevichv-at-umkc.edu
Date: Tue, 12 Aug 2003 09:53:39 -0500
Subject: RE: SEM - charging problems with bio samples

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I think it is better not to use conductive tape since anyway
you are using silver paint (I prefer to use graphite paint).
Next step is to coat for one additional minute at different
tilt angle - for specimens with rough surface it could be
helpful. Also you can try to rise voltage to 15-25 kV to
check if you can see gold with BSE on platinum coated specimens.

I have never used pure platinum for coating, but with two
coatings with gold/palladium (15 ma, 30 sec. each) I usually
get good results.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Phillips, Thomas E.
} Sent: Sunday, August 10, 2003 7:18 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: SEM - charging problems with bio samples
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} We are relative novices on the SEM and are seeking advice on ways to
} minimize our charging problems. We would appreciate any tips
} from the
} mavens out there.
}
} We are looking at biological specimens (tissue explants)
} coated either with
} platinum or carbon (details below). The carbon coating
} protocol is for
} when we are trying to image at colloidal gold labeling on the
} surface using
} the BSE detector. The charging is much worse on the carbon coated
} specimens compared to the platinum coated ones. I don't have
} a feel for
} how much of this problem is "normal" and one has to live
} with it like so
} much else in EM! But if anyone out there has some ideas or tricks to
} reduce charging, we are willing to try it. I would also be
} interested in
} anyone's nomination for a great reference book on SEM
} techniques. Thanks. Tom
}
} Platinum coating protocol:
}
} Tissue (2x2x2 mm) fixed in parformaldehyde/glutaraldehyde (or
} 2% PF) for
} several hours.
} Tissue osmicated with OsO4 in HEPES Wash Buffer for 2 hr.
} Tissue dehydrated in EtOH series.
} Tissue critical point dried.
} Tissue mounted on stub with conductive tape.
} Next, silver painted the edges of the specimen and allowed to dry.
} Sputter coated the specimen with platinum at 10 mA for 70 sec.
} Viewed on Hitachi SEM at 5 kV and 20 uA at magnifications
} ranging from
} 1-20k and sometimes up to 50k.
} Specimens stored in a desiccation chamber.
} *Re-coating for another minute fixes the problem some of the time.
}
} Carbon coating protocol:
}
} Tissue (from 1x1x1 to 3x3x3 mm) fixed in 2% PF for several hours.
} Tissue osmicated with OsO4 in HEPES Wash Buffer for 2 hr.
} Tissue dehydrated in EtOH series.
} Tissue critical point dried.
} Tissue mounted on stub with conductive tape.
} Next, silver painted the edges of the specimen and allowed to dry.
} Sputter coated the specimen with high purity carbon for 3 sec
} (~ 20 nm
} coating?).
} Viewed on Hitachi SEM at 5 kV and 20 uA at magnifications
} ranging from
} 1-20k using both BSE and SE to image.
} Specimens stored in a desiccation chamber.
}
}
} Thomas E. Phillips, PhD
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}
}


From daemon Tue Aug 12 10:34:28 2003



From: Gary Laevsky :      laevsky-at-scripps.edu
Date: Tue, 12 Aug 2003 08:27:08 -0700
Subject: thanks to all, nothing further

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html











Gary S. Laevsky, Ph.D.
Research Associate
The Scripps Research Institute
10550 N. Torrey Pines Road/IMM-24
La Jolla, CA 92037
(858) 784-9372




From daemon Tue Aug 12 10:42:19 2003



From: Vladislav Speransky :      vlad-at-linus.niams.nih.gov
Date: Tue, 12 Aug 2003 11:38:03 -0400
Subject: Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom (and Neil!),

Are you doing silver enhancement? If not, did you try gold-gilded
copper grids? That's what I use. Reasonably sturdy, don't do funny
things when approached with tweezers, not to mention astigmatism in
the scope, and they are not too expensive. Gold coating seems to
prevent any contact of Cu with the PBS.

I did have what seemed to be Ni-induced astigmatism a few times, but
it was always one particular grid, and the next Ni grid would be OK.

Vlad
--
-------------------------------------------
Vladislav V. Speransky, Ph.D.
Laboratory of Structural Biology
NIAMS, National Institutes of Health
50 South Drive, Room 1504
Bethesda, MD 20892-8025
Phone: 301 496-3989
Fax: 301 480-7629
E-mail: Vladislav_Speransky-at-nih.gov


From daemon Tue Aug 12 11:16:30 2003



From: Clarkson Donna R Contr USAMRD :      donna.clarkson-at-brooks.af.mil
Date: Tue, 12 Aug 2003 11:10:40 -0500
Subject: Formvar in Ethylene Dichloride

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning, Listers

I am going to make up a batch of Formvar in Ethylene Dichloride and
would like some suggestions, please.

* Having not heard back from my safety people yet--how are you safely
& properly disposing of the waste?
* What is the best/safest way to clean my glassware and stir bar
afterwards?
* What seems to be the best dilution? I had read to use between 0.1 %
and 0.3%, but could have sworn we used 0.75% in a lab years ago.

Thanks in advance for any helpful advice!

Donna R. Clarkson

Northrop Grumman Information Technology
for U S Army Medical Research Detachment
at Brooks City-Base
Phone (210) 536-1416
FAX (210) 536-1449
e-mail donna.clarkson-at-brooks.af.mil



From daemon Tue Aug 12 11:30:04 2003



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 12 Aug 2003 09:25:31 -0700
Subject: Administrivia: Updated SPAM Filters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor;
Great job on the SPAM filtering. My Intel inbox was clogged with 627 emails after 4 days at MSA, and it was 98% SPAM. Any chance you could submit your resume to the Intel IT department? They certainly could use your expertise!

John Mardinly
Phone: 408-765-2346
Pager: 877-277-1182


-----Original Message-----
} From: zaluzec-at-sparc5.microscopy.com
[mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Monday, August 11, 2003 6:12 AM
To: Microscopy-at-sparc5.microscopy.com


Colleagues...

There was another massive spam attack this weekend. I have
updated the filters, but I'm leerie of the fact that this may
catch additional valid postings.

If you receive a "rejected mail messages" please remember to
follow the directions you receive so that I can resolve the issues
and get you back into the system.


Cheers....

Nestor
Your Friendly Neighborhood SysOp



From daemon Tue Aug 12 11:51:44 2003



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Tue, 12 Aug 2003 11:46:33 -0500
Subject: Nickel grids and demagnetization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I appreciate all the advice on nickel grids potentially causing me the
problems with stigmation. I agree this is a likely culprit but that
doesn't explain why the problem is intermittent - some times I experience
no problems at all. I thought perhaps some batches of grids were
magnetized and some weren't but the consensus seems to be that the EM
itself will make them magnetic so that eliminates that possibility. I am
thinking about switching to gold grids but the cost is not
insignificant. I need to use formvar/carbon coated grids to support my
very unstable acrylic resin sections of plant material for the rigorous
immunolabeling protocol that I have to follow. Since i would like to avoid
making my own films, my choice of vendors is limited. It looks to me like
switching to gold will cost 40 or 50 cents more a grid. That would be
several hundred dollars a year for me so I would like to avoid it if
possible. Clearly Ni grids can't always be problematic or nobody would buy
them. Thanks for all the comments on this issue. Tom



Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From daemon Tue Aug 12 13:37:01 2003



From: MicroscopyToday :      microtod-at-optonline.net
Date: Tue, 12 Aug 2003 14:30:17 -0400
Subject: Journals and book sets available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

Wendy Clark, Librarian
Tennessee Valley Authority
Research Library
Muscle Shoals, AL 35661
256-386-2872
whclark-at-tva.gov

Has contacted me and informed me that the TVA has closed its microscopy
facility at her location and has a long list of relevant journals and books
that will be discarded if no one wants them. I suggested that she give me
the list and I would post it on the listserver.

The list is below. Sorry about the length. If you want anything on the list,
PLEASE contact Wendy.

Ron Anderson, Editor
Microscopy Today

JOURNALS AVAILABLE FOR DONATION:


Agricultural ammonia news, 1954 - 1967
Agricultural and biological chemistry, 1966 - 1991 (Tokyo)
Agricultural history, 1978 - 1989
Agricultural nitrogen news, 1967 - 1970
Ambio, 1976 - 1984
American laboratory, 1969 - 1990
Applied engineering in agriculture, 1985 - 1994
Archiv fur Acker- und Pflanzenbau und bodenkunde/Deutsche Demokratische
Republik, Deutsche Akademie der Landwirtschftswissenschaften zu Berlin, 1972
- 1987
Archives of environmental contamination and toxicology, 1994 - 1997
ASTM standardization news, 1973 - 1984
Bacteriological reviews, 1961 - 1975
Bibliography of agriculture, Section A, agricultural economics and rural
sociology, 1942 - 1943
Bibliography of agriculture, Section B, agricultural engineering, 1942 -
1943
Bibliography of agriculture, section C, entomology, 1942 - 1943
Bibliography of agriculture, section D, plant science, 1942 - 1943
Bibliography of agriculture, section E, forestry, 1942 - 1943
Bibliography of agriculture, section F, food processing and distribution,
1943
Bibliography of agriculture/U.S. Department of Agriculture, Library, 1943 -
1975
Biochemistry, 1962 - 1974
Biochemistry and cell biology, 1986 - 1987
Biological abstracts, 1945 - 1969
Biological conservation, 1968 - 1981
Biotechnology letters, 1982 - 1983; 1996 - 1998
The Botanical review, 1964 - 1988
Bulletin of environmental contamination and toxicology, 1994 - 1997
Bulletin of the Academy of Sciences of the USSR, Division of Chemical
Science, 1952 - 1991
Canadian chemical processing, 1952 - 1984
Canadian chemistry and process industries, 1944 - 1951
Canadian journal of botany, 1996 - 1997
Canadian journal of chemical engineering, 1967 - 1993
Canadian journal of forest research, 1971 - 1981
Canadian journal of plant science, 1969 - 1995
Canadian journal of research, Sec. B, 1942 - 1943
Canadian journal of research, Sec. B, Chemical sciences, 1944 - 1950
Chemical and engineering news, 1942 - 2002
Chemical engineering, 1947 - 2002
Chemical engineering progress, 1947 - 1995
Chemical engineering science, 1966 - 1982
Chemical market reporter, 1996 - 1999
Chemical marketing reporter, 1984 - 1996
Chemical processing, 1960 - 1986
Chemistry and industry, 1936 - 1988
Chemistry in Britain, 1965 - 1995
Control engineering, 1960 - 1988; 1992
Corrosion, 1954 - 1995
Corrosion science, 1966 - 1974
Dissertation abstracts international, A, the humanities and social sciences,
1969 - 1994
Ecology, 1971 - 1997
Energy progress, 1981 - 1988
Engineering news-record, 1980 - 1986
ENR, 1987 - 2002
Environmental affairs, 1971 - 1978
Environmental pollution, 1973 - 1979
Environmental pollution, Series A, Ecological and biological, 1980 - 1982
The Environmental professional: the official journal of the National
Association of Environmental Professionals, 1988 - 1995
Environmental progress, 1982 - 1994
Forest science, 1955 - 1995
Growth and change, 1970 - 1987
Hazardous waste consultant, 1984 - 1995
Inorganic and nuclear chemistry letters, 1969 - 1981
Instrumentation science & technology, 1994 - 1997
Instrumentation technology: the journal of the Instrumentation Society of
America, 1963 - 1978
InTech, 1979 - 1992
International chemical engineering, 1961 - 1994
International journal of chemical kinetics, 1969 - 1989
International journal of sulfur chemistry, 1973 - 1976
International journal of sulfur chemistry, A, Original, experimental, and
theoretical studies, 1971 - 1972
International journal of sulfur chemistry, B, quarterly reports on sulfur
chemistry, 1971 - 1972
International journal of sulfur chemistry, C, Mechanisms of reactions of
sulfur compounds, 1971 - 1972
Journal of analytical atomic spectrometry, 1986 - 1995
Journal of applied spectroscopy, 1965 - 1972
Journal of chemical engineering of Japan, 1968 - 1982
The Journal of chemical thermodynamics, 1969 - 1993
Journal of inorganic and nuclear chemistry, 1955 - 1981
Journal of regional science, 1958 - 1988
Journal of research of the National Bureau of Standards, 1977 - 1988
Journal of research of the National Bureau of Standards, 1934 - 1959
Journal of research of the National Bureau of Standards, B, Mathematical
sciences, 1968 - 1977
Journal of research of the National Bureau of Standards, B, mathematics and
mathematical physics, 1959 - 1967
Journal of research of the National Bureau of Standards, C, engineering and
instrumentation, 1959 - 1972
Journal of research of the National Bureau of Standards, Section A, Physics
and chemistry, 1959 - 1977
Journal of research of the National Institute of Standards and Technology,
1989 - 1995
Land and water: the magazine of natural resource management and
restoration, 1994 - 1998
Langmuir: the ACS journal of surfaces and colloids, 1985 - 1995
Mendeleev chemistry journal 1983 - 1989
Nuclear instruments & methods, 1967 - 1981
Nuclear instruments & methods in physics research, 1981 - 1983
Nuclear instruments & methods in physics research, Section A, accelerators,
spectrometers, detectors, and associated equipment, 1984 - 1988
Nuclear instruments & methods in physics research, Section B, Beam
interactions with materials and atoms, 1984 - 1989
Pesticide science, 1992 - 1995
Phosphorus and sulfur and the related elements, 1976 - 1988
Phosphorus and the heavier group Va elements, 1971
Phosphorus and the related group V elements, 1972 - 1975
Phosphorus, sulfur, and silicon and the related elements, 1989 - 1991
Pollution engineering, 1969 - 1995
Proceedings of the Academy of Sciences of the USSR, 1965 - 1990
Proceedings of the Analytical Division of the Chemical Society, 1975 - 1979
Proceedings of the Chemical Society, 1957 - 1964
The Progressive fish-culturist, 1947 - 1968, 1992 - 1994
The quarterly journal of economics, 1964 - 1987
Quarterly reports on sulfur chemistry, 1966 - 1970
Quarterly reviews - chemical society, 1960 - 1971
Record of chemical progress, 1939 - 1971
Recueil des travaux chimiques des Pays-Bas, 1920 - 1991
Recueil: journal of the Royal Netherlands Chemical Society, 1980 - 1991
Remote sensing of environment, 1969 - 1981
The Science of the total environment, 1994 - 1995
Sensors and actuators, B, Chemical, 1992 - 1997
Separation and purification methods, 1972 - 1983
Separation science, 1966 - 1977
Soviet Materials Science, 1965 - 1981
Soviet Physics, Crystallography, 1969 - 1982
Soviet Plant Physiology, 1962 - 1982
Soviet Progress in Chemistry, 1984 - 1991
Standardization news, 1985 - 1992
Studies in applied mathematics, 1969 - 1987
Successful farming, 1960 - 1995
Synthesis and reactivity in inorganic and metal-organic chemistry, 1974 -
1988
Synthesis in inorganic and metal-organic chemistry, 1971 - 1973
Tree physiology, 1986 - 1994
Tree planters' notes, 1967 - 1994
Weed Science, 1996 - 1998
Weed technology: a journal of the weed science society of America, 1996 -

BOOK SETS

Annual Review of Plant Physiology v.15, 1964 - V48, 1987
Handbuch der Pflanzenphysiologie (Encyclopedia of Plant Physiology) 18v.
SpringerVerlag, 1967 (German-English)
Plant Physiology v.1-5. ed. F.C. Steward. Academic, 1960
Annual Review of Phytopathlogy v.4, 1966-v.31,1993
Annual Review of Microbiology v.14, 1960-v.47, 1993
Annual Review of Entomology v.1,1956-v.37,1992
Annual Review of Biochemistry v.30,1961-v.55,1986
Industrial Waste Conference. Purdue University. 2nd,1946-48th,1993
Proceedings American Society for Horticultual Science v.36,1938; v.53,1949 -
v.93,1968
Proceedings Annual Convention, Association of Land Grant Colleges &
Universities. 47, 1933 - 80th, 1966.
Florida State Horticultural Society Proceedings v.75,1962 - v.100, 1987
Proceedings Agricultural Research Institute, 9th, 1960 - 32nd, 1983
Transactions of the International Congress of Soil Science 1st,1927; 2nd,
1930; 3rd., 1956; 7th, 1960; 8th, 1964; 9th, 1968; 10th, 1974; 11th, 1978;
12th., 1982; 13th, 1986.
Proceedings Soil Science Society of Florida, v.1, 1939 - v.53, 1994




From daemon Tue Aug 12 13:59:58 2003



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 12 Aug 2003 13:54:50 -0500
Subject: Re: Formvar in Ethylene Dichloride

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


QUESTION FROM:

} Donna R. Clarkson
} Northrop Grumman Information Technology
} for U S Army Medical Research Detachment
} at Brooks City-Base
} Phone (210) 536-1416
} FAX (210) 536-1449
e-mail donna.clarkson-at-brooks.af.mil

} I am going to make up a batch of Formvar in Ethylene Dichloride and
} would like some suggestions, please.
}
} * Having not heard back from my safety people yet--how are you safely
& properly disposing of the waste?

Work in a fume hood as the fumes are considered carcinogenic. Keep
all wastes in a container (vented to hood and away from flames) for
disposal as a volatile chemical.

} * What is the best/safest way to clean my glassware and stir bar
} afterwards?

Rinse in ethylene dichloride several times and put the washes in the
disposal container, above.

} * What seems to be the best dilution? I had read to use between 0.1 %
} and 0.3%, but could have sworn we used 0.75% in a lab years ago.
}
I have used 0.25% Formvar or Butvar in ethylene dichloride. My
recommendation is that you purchase the solution already made up (all
EM Supply houses carry it). That way you have less waste and the
product is guaranteed.

JB

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Tue Aug 12 15:03:01 2003



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Tue, 12 Aug 2003 15:56:57 -0400
Subject: gfp labelled cells & immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

I have been trying to label V5 postive neurons of 4.0% paraform. fixed brain
sections with a polyclonal V5 antibody. These neurons are also gfp (green
fluores. protein) labelled so we know that the vector has delivered the V5
sequence. The gfp is not fused to the V5 coding sequence.

My question is does the gfp fluores. inhibit any labeling with the
avidin-biotin immunocytochemistry procedure to labelling with the V5
antibody (like steric hindrance which is sometimes a problem with gold
immunoEM labelling)? So far we have tried 3 times to label with no success.
We make sure we have gfp positive cells in the brain tissue slices before we
start any immunocytochemistry procedure.

I am using DAB (diaminobenzidine) as a chromagen which would be silver
enhanced and then the 70 micron brain tissue slab would be post fixed in
2.0% glut, osmicated and embedded in Epon.

Any help would be very much appreciated!

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642




From daemon Tue Aug 12 18:39:48 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 12 Aug 2003 16:26:17 -0700
Subject: Re: gfp labelled cells & immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen

GFP may not interfere with biotin-avidin reaction. The constant of
association for biotin-avidin complex is about 10^12M^-1, which is pretty
high. 4% formaldehyde is sounds to me too much. It may kill antigenic
determinants if you are using primary antibodies. From another hand
(opposite to the previous), V5 may be easily washed out if it's small
peptide (have no idea what it is). I would suggest to do immuno-staining
at LM level first to be sure that your procedure works, then switch to
EM. Brain, also contains a lot of lipids, so you may need to use
detergents to open cells up. Good luck, Sergey.

At 12:56 PM 8/12/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Aug 12 22:28:05 2003



From: Steve Parry :      sparry-at-cmm.uwa.edu.au
Date: Wed, 13 Aug 2003 11:18:54 +0800
Subject: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have recently switched to Formvar in Diethylene Chloride as a base
for making carbon coated grids, because I was told that I would get
bettter continuous films, the problem I am having is that I cannot
get rid of the forvar after coating.
Does anyone out there have a method for making C coated grids that
works everytime?? I am getting to the end of my patience and have
wasted an awful lot of grids over the last few months.

Thanks

Steve Parry
--
Steve Parry
Centre for Microscopy & Microanalysis, (M010)
The University of Western Australia,
35 Sterling Highway, Crawley,
WA 6009, Australia.

Fax: +61-8-9380-1087
Email: sparry-at-cmm.uwa.edu.au
Phone: +61-8-9380-8057 [24 hour voicemail attached]



From daemon Wed Aug 13 05:30:51 2003



From: Klijn, F. (Frits) :      Frits.Klijn-at-akzonobelcatalysts.com
Date: Wed, 13 Aug 2003 12:22:04 +0200
Subject: fluorescence micorscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues

Does anybbody have some experiences in the application of fluorescents in combination with silica/alumina based catalysts?
What type of fluorescents? Etc.

I am quite new in this field so all information is welcome.

Regards,

Frits Klijn
Akzo Nobel Catalysts b.v.
Dept. SMA - R&D1
P.O.Box 37650
1030 BE Amsterdam
Netherlands

Phone : +31 - (0)20 - 6347962 / 7981
Fax : +31 - (0)20 - 6347678


****************************************************
This message, including attachments, is confidential and may be privileged. If you are not an intended recipient, please notify the sender then delete and destroy the original message and all copies.
You should not copy, forward and/or disclose this message, in whole or in part, without permission of the sender.
****************************************************




From daemon Wed Aug 13 06:18:15 2003



From: frederico cunha :      cunhaf-at-fisica.ufs.br
Date: Wed, 13 Aug 2003 08:05:28 -0300
Subject: STM: noise reduction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Listers:

I am currently using a CP-Research AFM/STM unit from
ThermoMicroscopes/Veeco. I would like to find someone who has made
improvements in the machine for electronic noise reduction in the STM
mode. I notice that when the tip is retracted, a “leakage” current of
hundreds of picoamps can be measured. While scanning, I can only
stabilize the image using three nanoamps or more.


thank you

Prof. Dr. Frederico Cunha
Physics Department
Universidade Federal de Sergipe
Brazil



From daemon Wed Aug 13 09:51:59 2003



From: Charles Murphy :      murphyc-at-ba.ars.usda.gov
Date: Wed, 13 Aug 2003 10:34:54 -0400
Subject: Philips400 T Free to good home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello: We are getting rid of a Philips 400T TEM. It was in working order as of last year. It has been listed on Govt' surplus for a few months with no takers. It is now being offered to Universities, and other similar organization. You will have to pay for packing and shipping. We are located in Maryland. Drop me an email if interested, Charlie Murphy murphyc-at-ba.ars.usda.gov

Charles Murphy
USDA, ARS,
Electron Microscopy Unit
Bldg. 177B, BARC East
Beltsville, MD, 20705
(301) 504-8046
(301) 504-8923 fax
murphyc-at-ba.ars.usda.gov






From daemon Wed Aug 13 11:00:25 2003



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 13 Aug 2003 09:06:23 -0700
Subject: Re: Formvar in Ethylene Dichloride

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Tuesday, August 12, 2003, at 09:10 AM, Clarkson Donna R Contr USAMRD
wrote:

} * Having not heard back from my safety people yet--how are you safely
} & properly disposing of the waste?
}
Dear Donna,
I second what John Bozzola said, and check the local regulations for
proper disposal of Cl-containing hydrocarbons. In NY they were
separated from other HCs, but in CA they are not. In both cases, the
safety offices took care of the disposal.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From daemon Wed Aug 13 12:30:21 2003



From: Mike Delannoy :      delannoy-at-jhmi.edu
Date: Wed, 13 Aug 2003 13:24:27 -0400
Subject: Re: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,
When I want just carbon as a substrate I will
coat freshly cleaved mica with carbon in a metal
evaporator. This allows me to dictate the carbon
thickness and I have no trouble floating off grid
size squares(prescored)in a drop of water. Picking up the
carbon with glow discharged grids (flat side)
is a snap. Make sure everything is clean.
This should give you thin clean substrates.
Mike D.

Steve Parry wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have recently switched to Formvar in Diethylene Chloride as a base
} for making carbon coated grids, because I was told that I would get
} bettter continuous films, the problem I am having is that I cannot
} get rid of the forvar after coating.
} Does anyone out there have a method for making C coated grids that
} works everytime?? I am getting to the end of my patience and have
} wasted an awful lot of grids over the last few months.
}
} Thanks
}
} Steve Parry
} --
} Steve Parry
} Centre for Microscopy & Microanalysis, (M010)
} The University of Western Australia,
} 35 Sterling Highway, Crawley,
} WA 6009, Australia.
}
} Fax: +61-8-9380-1087
} Email: sparry-at-cmm.uwa.edu.au
} Phone: +61-8-9380-8057 [24 hour voicemail attached]



From daemon Wed Aug 13 13:24:44 2003



From: Pombo, Ana :      ana.pombo-at-csc.mrc.ac.uk
Date: Wed, 13 Aug 2003 19:18:05 +0100
Subject: LM: Job opportunity at MRC-CSC Microscopy Core Facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Research Associate
 
Based in West London, the Clinical Sciences Centre (CSC) is an Institute of
the Medical Research Council and an academic division of the Faculty of
Medicine, Imperial College, London, UK. The CSC has an international
reputation and its interdisciplinary research and training programmes in
cell biology, genomics, and biological and clinical imaging, address key
questions underpinning human health and disease.
 
The imaging of molecules within cells, organs and whole organisms is at the
forefront of scientific developments in the life sciences. The CSC is a
leading research institute with groups developing new avenues in the areas
of confocal laser scanning microscopy (CLSM), live-cell imaging (CCD imaging
and spinning-disk confocal), transmission electron microscopy (TEM),
scanning ion conductance microscopy, position emission tomography (PET), and
nuclear magnetic resonance (NMR).
 
We are seeking a highly motivated individual to be responsible for this
state-of-the-art microscope core facility at the CSC, to provide full
support for confocal, live-cell imaging, and other fluorescence microscope
users within the institute. Duties will include direct technical support,
training and supervision of users, assuring instrument integrity,
maintaining the facility web site. Direct involvement in research project
using fluorescence microscopy will be expected (further information about
research at the CSC can be found at www.csc.mrc.ac.uk ). The candidate will
have to maintain a current understanding of the microscopy field as
necessary to implement new technologies in the department.
 
The successful candidate should have a science degree in a relevant subject,
with 5 years research experience or a PhD in a relevant subject, and must
demonstrate a good understanding of current fluorescence imaging technology
and sample preparation. Experience in confocal, live-cell and digital
imaging (particularly Leica and Perkin-Elmer confocal microscopes and/or
Deltavision microscope) are highly desirable.   Applicants at this level
will be appointed at MRC Pay Band 4.  Please quote Ref: MRA/RA/D.
 
We also actively encourage applications from candidates with less experience
but a minimum of 3 years of research experience or imaging facility
management who will run the facility and assist in research projects. 
Applicants should have a science degree in a relevant subject and will be
appointed at MRC Pay Band 5.  Please quote Ref: MRA/RAS/D.
 
All applicants must have a strong wish to participate actively in team work,
possess excellent written and oral communication skills, be comfortable
working independently, communicate effectively with service users to
understand their microscopy needs, be at ease with PC computer platforms and
demonstrate effective troubleshooting skills.
 
Further details on how to apply are available from www.csc.mrc.ac.uk or
email recruit-at-csc.mrc.ac.uk, quoting the relevant reference above.  Informal
enquiries and further information about the position to Drs Ana Pombo (tel:
020 8383 8232; ana.pombo-at-csc.mrc.ac.uk) or Alex Sardini (tel: 020 8383 8270,
a.sardini-at-csc.mrc.ac.uk).
 
The closing date for applications is 20 September 2003.
Interviews will take place 22 September-15 October 2003.


Ana Pombo, D.Phil.
Group Head
Nuclear Organisation Group
MRC Clinical Sciences Centre
Hammersmith Hospital Campus
Du Cane Road
London
W12 0NN
UK
 
Tel. office - (+0/44) 20 83838232
Tel. lab    - (+0/44) 20 83833984
Fax         - (+0/44) 20 83838306
Email      - ana.pombo-at-csc.mrc.ac.uk



From daemon Wed Aug 13 17:38:30 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 13 Aug 2003 15:32:30 -0700
Subject: Re: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to add a few cents to the Mike Delannoy posting. Instead
picking carbon on individual grid in the drop of water, I am doing as a
following:

- you need some "flask" diameter about 90 mm and 40-50 mm high with
deionized ultrapure water filled up to about 5 mm lower than edge (flask
itself should be clean as well). You also may use any suitable in size
glass container. The idea is to have relatively large flat area of water
surface.
- you need fluorescent lamp to illuminate the water surface in the way it
happening when you illuminate water surface in the ultratome knife
boat. So, you should see the reflection of the bulb on the
surface. Playing with the angle ant location, you need to find the
orientation, which gives you well illuminated large area on the water surface.
- depends, how many grids you want to made, cut the piece of mica with
carbon of correspondent size. Important: all FOUR sides of your mica piece
should be fresh cut.
- slowly under 45 deg angle move mica (carbon is up) into the water, so
carbon will float on the surface. It should be visible if illumination is
set right.
- put grids on the floating carbon. DO NOT touch carbon by tweezers. Try
to drop grids on the carbon from a few mm distance.
- cut the piece of Parafilm slightly bigger that carbon. Using two
tweezers, very carefully put Parafilm on the carbon (with grids). Hold one
side of the Parafilm sheet with tweezer; with one smooth movement move
Parafilm (with carbon and grids) under the water and then out of water.
- place Parafilm sheet with attached grids (up) on the piece of filter
paper and dry at 60oC for about 10 min.
-Grids are ready.
- Personally, I prefer to use fresh carbon for every experiment, so I made
about 6 grids at the time and them make more if needed. If you are using
extremely thick carbon film, you may not need good illumination.
- I don't see any point to glow discharge grids itself. If glow discharge
"works" on the naked grids, it simply meant that grids are greasy and that
contamination has been ionized by glow discharge. Clean metal grids will
not hold charge at all (electro-conductivity, you know). Covering grids
with very diluted plastic solution (like parlodium 0.01-0.005%) may help to
attach carbon to the grids. Just put grids on the filter paper and put a
drop of plastic on it, let it dry. More effective way to mount carbon film
on the grids is to use "holey" carbon coated grids. Carbon sticks nicely
to carbon.

Sergey


At 10:24 AM 8/13/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Aug 13 17:45:23 2003



From: firmiss :      firmiss-at-granicus.if.org
Date: Wed, 13 Aug 2003 22:42:26 +0000
Subject: Re: SEM - charging problems with bio samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Sun, Aug 10, 2003 at 07:17:56PM -0500, Tom Phillips wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We are relative novices on the SEM and are seeking advice on ways to
} minimize our charging problems. We would appreciate any tips from the
} mavens out there.
}
} We are looking at biological specimens (tissue explants) coated either with
} platinum or carbon (details below). The carbon coating protocol is for
} when we are trying to image at colloidal gold labeling on the surface using
} the BSE detector. The charging is much worse on the carbon coated
} specimens compared to the platinum coated ones. I don't have a feel for
} how much of this problem is "normal" and one has to live with it like so
} much else in EM! But if anyone out there has some ideas or tricks to
} reduce charging, we are willing to try it. I would also be interested in
} anyone's nomination for a great reference book on SEM techniques. Thanks.
} Tom

I only know a little bit about colloidal gold labeling but I would think
if you're using a BSE detector to look for the gold then I don't think
you'd want to coat the sample with gold or platinum (unless the gold
particles are significant topographic features and you're using the
BSE detector in 'topo' mode) -- that pretty much leaves you with carbon
coating -- more prone to charging but still usable.

Here are some general SEM techniques you can try to reduce charging for
'problem' samples.

* Use smaller spot size
* Use smaller aperature
* Use a LOWER magnification if feasable
* Increase scan rate

All these basically reduce the rate at which electrons hit a given area
of your sample.

If you're still having problems you may have to coat with more carbon.

I hope this helps. Good luck!

--
James Firmiss ------ firmiss-at-granicus.if.org ------
"Let Sanity Prevail"


From daemon Wed Aug 13 18:50:00 2003



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 14 Aug 2003 08:15:06 +0100
Subject: SEM - charging problems with bio samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Steve,
The method I use to make carbon-coated grids uses collodion in amyl acetate. A
drop or two is put onto the surface of a large dish of distilled water. This
dries to a circle about three to four inches in diameter on the surface of the
water. I drop copper grids onto this surface, preferably before it dries too
much, so the collodion is still a bit sticky. After I have about thirty grids
arranged in a neat rectangle on the collodion film, I drop a filter paper on top
and bring the edges of the film over the edges of the filter paper. As soon as
the paper is wet, I lift it out by one edge and lay it down, grids up, on more
filter paper to dry. This may take some practice to get the filter paper up out
of the water with the grids still stuck on. When the paper is dry, I cut out the
rectangle of paper with the grids on it with scissors and carbon coat it (grids
side up) in the evaporator.
The grids are then removed from the paper and put in a Jaffe washer
(collodion/carbon side up) filled with chloroform for 48 hours. (A Jaffe washer
is a petri plate with a stack of four glass slides and a stack of filter paper
over the glass slides. The bottom of the petri plate is filled with solvent and
the lid of the petri plate put on. Then, another, larger dish is put over it so
the solvent doesn't evaporate too quickly. The material put on the top of the
filter paper stays wet with solvent and the solvent washes the material). This
will dissolve the collodion and bring the carbon film down to stick it to the
copper grid. These films are conductive, continuous and strong enough for
routine work at 200kV.
Good luck,
Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "Steve Parry" {sparry-at-cmm.uwa.edu.au}
To: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, August 12, 2003 8:18 PM


James/Tom
Yes, it is certainly my experience that gold palladium sputter
coating
quickly kills
the BSE signal from 5 and 10nm colloidal gold. Carbon provides the
best signal from small gold, but a thin sputtered chromium coating is
also very good, and has the advantage of providing more fine
topographical detail than can be obtained from carbon-coated
specimens. I have used the Denton head in my Gatan Alto to do this,
and have also had excellent results from specimens sputtered for us
by
Emitech.

Unfortunately, the techniques James suggests for reducing charging,
while effective for reducing charging, may be just exactly what you
want to avoid in BSE imaging of gold. Signal and signal/noise is
often
a problem, so one needs more beam current rather than less, and long
record times, and it is therefore important that the coating and
mounting technique is perfect. I often use the Analysis mode with the
normal aperture 2/3 and and a relatively large spot size 5 on my
Hitachi 4700, with the emission current increased to 15 or even 20µA,
and find I need to be working at higher mags (say 20-100k) to get the
best images.

Things to consider when optimizing carbon coating and specimen
mounting are:

*Use rotary-tilt coating technique to get even coating all over.
Static coating will leave a 3-d specimen with large uncoated shadow
areas
*Make sure the coating really is thick enough. A piece of white paper
or masking tape placed near the specimens should be mid grey
*Ensure the coating is in electrical continuity with the stub. If
there is the slightest gap under the tissue block then paint it up
with carbon dag.
*If the specimens are large cubes (} 1mm) make sure the sides are
adequately coated with carbon, and if in doubt dag them
*Paint dag lines to the metal of the stub. Don't rely entirely on the
conductivity of sticky carbon tabs, which is often not good enough.

Chris

Dr. Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility

} ----- Original Message -----
} From: "firmiss" {firmiss-at-granicus.if.org}
} To: "MSA ListServer" {Microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, August 13, 2003 11:42 PM
} Subject: Re: SEM - charging problems with bio samples
}
}
}
} --------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
} ---.
} }
} }
} } On Sun, Aug 10, 2003 at 07:17:56PM -0500, Tom Phillips wrote:
} }
}
} --------------------------------------------------------------------
} ----
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe --
} } } http://www.msa.microscopy.com/MicroscopyListserver
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} --------------------------------------------------------------------
} ---.
} } }
} } }
} } } We are relative novices on the SEM and are seeking advice on
ways
} to
} } } minimize our charging problems. We would appreciate any tips
from
} the
} } } mavens out there.
} } }
} } } We are looking at biological specimens (tissue explants) coated
} either with
} } } platinum or carbon (details below). The carbon coating protocol
} is for
} } } when we are trying to image at colloidal gold labeling on the
} surface using
} } } the BSE detector. The charging is much worse on the carbon
coated
} } } specimens compared to the platinum coated ones. I don't have a
} feel for
} } } how much of this problem is "normal" and one has to live with
it
} like so
} } } much else in EM! But if anyone out there has some ideas or
tricks
} to
} } } reduce charging, we are willing to try it. I would also be
} interested in
} } } anyone's nomination for a great reference book on SEM
techniques.
} Thanks.
} } } Tom
} }
} } I only know a little bit about colloidal gold labeling but I would
} think
} } if you're using a BSE detector to look for the gold then I don't
} think
} } you'd want to coat the sample with gold or platinum (unless the
gold
} } particles are significant topographic features and you're using
the
} } BSE detector in 'topo' mode) -- that pretty much leaves you with
} carbon
} } coating -- more prone to charging but still usable.
} }
} } Here are some general SEM techniques you can try to reduce
charging
} for
} } 'problem' samples.
} }
} } * Use smaller spot size
} } * Use smaller aperature
} } * Use a LOWER magnification if feasable
} } * Increase scan rate
} }
} } All these basically reduce the rate at which electrons hit a given
} area
} } of your sample.
} }
} } If you're still having problems you may have to coat with more
} carbon.
} }
} } I hope this helps. Good luck!
} }
} } --
} } James Firmiss ------ firmiss-at-granicus.if.org ------
} } "Let Sanity Prevail"
} }
}



From daemon Thu Aug 14 03:10:37 2003



From: James Chalcroft :      jchalcro-at-neuro.mpg.de
Date: Thu, 14 Aug 2003 10:05:45 +0200
Subject: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Steve,

I second Mike Delannoy's suggestion. Mica should give you the smoothest
continuous carbon films.
In my experience one problem is that the C-films can wrinkle terribly if
floated off too soon from the mica.
This can be simply avoided by letting the film first "mature" overnight
on the mica.
Good luck,

Jim

-----Original Message-----
} From: Steve Parry [mailto:sparry-at-cmm.uwa.edu.au]
Sent: Wednesday, August 13, 2003 5:19 AM
To: Microscopy Listserver


I have recently switched to Formvar in Diethylene Chloride as a base
for making carbon coated grids, because I was told that I would get
bettter continuous films, the problem I am having is that I cannot
get rid of the forvar after coating.
Does anyone out there have a method for making C coated grids that
works everytime?? I am getting to the end of my patience and have
wasted an awful lot of grids over the last few months.

Thanks

Steve Parry
--
Steve Parry
Centre for Microscopy & Microanalysis, (M010)
The University of Western Australia,
35 Sterling Highway, Crawley,
WA 6009, Australia.

Fax: +61-8-9380-1087
Email: sparry-at-cmm.uwa.edu.au
Phone: +61-8-9380-8057 [24 hour voicemail attached]




From daemon Thu Aug 14 09:43:54 2003



From: JHoffpa464-at-aol.com
Date: Thu, 14 Aug 2003 10:36:33 -0400
Subject: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


why not just buy the carbon coated grids. much simpler.


From daemon Thu Aug 14 09:47:02 2003



From: Christopher Gilpin :      christopher.gilpin-at-utsouthwestern.edu
Date: Thu, 14 Aug 2003 09:43:34 -0500
Subject: EM autoradiography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

We have an EM autoradiography project to carry out. It is way too long
since I did any of this so I need some advice about exposure times. If
anyone is current on AR please contact me offline if you can help.
It was great to see everyone again in San Antonio - well done MSA for
another great meeting.

Regards


Chris

Christopher J. Gilpin Ph.D.
Assistant Professor
Director Molecular and Cellular Imaging Facility
K1.246
Department of Cell Biology
University of Texas Southwestern Medical Center
5323 Harry Hines Boulevard
Dallas, Texas 75390-9039
+1 214 648 2827 Phone
+1 214 648 6408 Fax
christopher.gilpin-at-utsouthwestern.edu



From daemon Thu Aug 14 10:26:17 2003



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Thu, 14 Aug 2003 10:19:19 -0500
Subject: grid glue recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anybody know a quick recipe for "grid glue" made out of scotch tape
(or other sticky stuff) and some solvent for glueing films to TEM
grids? Thanks in advance.
-Karl

--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu




From daemon Thu Aug 14 10:26:18 2003



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Thu, 14 Aug 2003 11:16:19 -0400
Subject: How to simulate glow discharge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey little Gloworms,

I am attempting to do a negative stain (which I know how to do) on a sample. the problem I'm having is that according to the protocol I have to glow discharge the grid before I use it.

I don't have access to a vacuum evaporator with a glow discharge unit. So what's a tech to do?

Any suggestions as to how to fake a glow are greatly appreciated.

Please help me to glimmer, glimmer. ;-)


Unable to glow,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Electron Microscope Lab
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax


From daemon Thu Aug 14 10:31:16 2003



From: Shane Roberts :      roberts-at-southbaytech.com
Date: Thu, 14 Aug 2003 08:27:36 -0700
Subject: SEM - charging problems with bio samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have had a lot of success using ion beam sputter deposited Cr thin
films for colloidal gold labeling of biological samples. Due to the
nature of ion beam sputtered films we can precisely control the
deposited thickness to less than 10 angstroms and still provide a
uniform layer of Cr for charge reduction. The gold label on the sample
provide high contrast in backscatter mode and the deposited Cr does not
interfere with the signal.

If you would like additional information on this type of equipment or
would like to see some examples I would be happy to provide this to you
off-list. I hope this helps!

Best Regards,

Shane Roberts
Applications Engineering Manager
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673
www.southbaytech.com
roberts-at-southbaytech.com


DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.


-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Thursday, August 14, 2003 12:15 AM
To: microscopy-at-sparc5.microscopy.com


James/Tom
Yes, it is certainly my experience that gold palladium sputter coating
quickly kills the BSE signal from 5 and 10nm colloidal gold. Carbon
provides the best signal from small gold, but a thin sputtered chromium
coating is also very good, and has the advantage of providing more fine
topographical detail than can be obtained from carbon-coated specimens.
I have used the Denton head in my Gatan Alto to do this, and have also
had excellent results from specimens sputtered for us by Emitech.

Unfortunately, the techniques James suggests for reducing charging,
while effective for reducing charging, may be just exactly what you
want to avoid in BSE imaging of gold. Signal and signal/noise is often
a problem, so one needs more beam current rather than less, and long
record times, and it is therefore important that the coating and
mounting technique is perfect. I often use the Analysis mode with the
normal aperture 2/3 and and a relatively large spot size 5 on my
Hitachi 4700, with the emission current increased to 15 or even 20µA,
and find I need to be working at higher mags (say 20-100k) to get the
best images.

Things to consider when optimizing carbon coating and specimen
mounting are:

*Use rotary-tilt coating technique to get even coating all over.
Static coating will leave a 3-d specimen with large uncoated shadow
areas *Make sure the coating really is thick enough. A piece of white
paper or masking tape placed near the specimens should be mid grey
*Ensure the coating is in electrical continuity with the stub. If there
is the slightest gap under the tissue block then paint it up with
carbon dag. *If the specimens are large cubes (} 1mm) make sure the
sides are adequately coated with carbon, and if in doubt dag them
*Paint dag lines to the metal of the stub. Don't rely entirely on the
conductivity of sticky carbon tabs, which is often not good enough.

Chris

Dr. Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility

} ----- Original Message -----
} From: "firmiss" {firmiss-at-granicus.if.org}
} To: "MSA ListServer" {Microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, August 13, 2003 11:42 PM
} Subject: Re: SEM - charging problems with bio samples
}
}
}
} --------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
} ---.
} }
} }
} } On Sun, Aug 10, 2003 at 07:17:56PM -0500, Tom Phillips wrote:
} }
}
} --------------------------------------------------------------------
} ----
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe --
} } } http://www.msa.microscopy.com/MicroscopyListserver
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} --------------------------------------------------------------------
} ---.
} } }
} } }
} } } We are relative novices on the SEM and are seeking advice on
ways
} to
} } } minimize our charging problems. We would appreciate any tips
from
} the
} } } mavens out there.
} } }
} } } We are looking at biological specimens (tissue explants) coated
} either with
} } } platinum or carbon (details below). The carbon coating protocol
} is for
} } } when we are trying to image at colloidal gold labeling on the
} surface using
} } } the BSE detector. The charging is much worse on the carbon
coated
} } } specimens compared to the platinum coated ones. I don't have a
} feel for
} } } how much of this problem is "normal" and one has to live with
it
} like so
} } } much else in EM! But if anyone out there has some ideas or
tricks
} to
} } } reduce charging, we are willing to try it. I would also be
} interested in
} } } anyone's nomination for a great reference book on SEM
techniques.
} Thanks.
} } } Tom
} }
} } I only know a little bit about colloidal gold labeling but I would
} think
} } if you're using a BSE detector to look for the gold then I don't
} think
} } you'd want to coat the sample with gold or platinum (unless the
gold
} } particles are significant topographic features and you're using
the
} } BSE detector in 'topo' mode) -- that pretty much leaves you with
} carbon
} } coating -- more prone to charging but still usable.
} }
} } Here are some general SEM techniques you can try to reduce
charging
} for
} } 'problem' samples.
} }
} } * Use smaller spot size
} } * Use smaller aperature
} } * Use a LOWER magnification if feasable
} } * Increase scan rate
} }
} } All these basically reduce the rate at which electrons hit a given
} area
} } of your sample.
} }
} } If you're still having problems you may have to coat with more
} carbon.
} }
} } I hope this helps. Good luck!
} }
} } --
} } James Firmiss ------ firmiss-at-granicus.if.org ------
} } "Let Sanity Prevail"
} }
}







From daemon Thu Aug 14 11:49:09 2003



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 14 Aug 2003 12:42:28 -0400
Subject: TIRF evaluation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We've been looking at the Nikon and Olympus TIRF systems.

Question 1:
Is the 100X 1.65 N.A. objective really necessary?

Question 2:
I would love to have an informal discussion off list with anybody who has
experience with one or both of these systems. If I may speak with you,
please contact me off list by email and we could write or set up a time
that I could call you at your convenience.

Thanks!
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/




From daemon Thu Aug 14 13:26:40 2003



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Thu, 14 Aug 2003 14:19:16 -0400
Subject: Light/Confocal Microscopist Position in Maine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have the following position available:

Light/ Confocal Microscopist: There is a full-time opening for a core
facility light/confocal microscopist available immediately. The encumbent
must demonstrate total facility with current technology and sample
preparations. They will be responsible for 1) providing direct hands-on
access to light microscopy in a core facility setting, 2) overseeing daily
facility operations, 3) consulting with users on experiment design,
materials and methods, and instrument optimization and assist in data
acquisition and analysis, 4) troubleshooting problems with microscopes or
samples and 5) assuring instrument integrity. Must maintain a current
understanding of the microscopy field as necessary to implement new
technologies on campus. Applicants must have a strong desire to participate
actively as a member of a team, possess excellent written and oral
communication skills, be comfortable working independently, communicate
effectively with service users to understand their microscopy needs, be
facile with PC and Mac computer platforms and demonstrate effective
troubleshooting skills. M.S. in biological sciences with five years
research experience in molecular biology, immunohistochemistry and cytology
is required. Previous work in a core facility is preferred.

The Jackson Laboratory is one of the world's foremost centers for mammalian
genetics research. Located in Bar Harbor, Maine, the lab is adjacent to
Acadia National Park. Mountains, ocean, forests, lakes, and trails are all
within walking distance. If you are looking for a more natural environment,
this could be the opportunity you've been searching for.

Interested applicants should send cover letter and resume to:
jax/210
Human Resources, Box 27
The Jackson Laboratory
600 Main Street
Bar Harbor, Maine 04609
Fax: (207) 288-6106
Email (preferred option): jobs-at-jax.org
The Jackson Laboratory is an Affirmative Action/Equal Opportunity Employer.


Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Thu Aug 14 14:06:22 2003



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 14 Aug 03 15:00:36 -0500
Subject: Glow discharge treatment in a plasma etcher

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-- [ From: Garber, Charles A. * EMC.Ver #3.1a ] --

Paula Sicurello wrote:
==================================================================
I am attempting to do a negative stain (which I know how to do) on a sample.
the problem I'm having is that according to the protocol I have to glow
discharge the grid before I use it.

I don't have access to a vacuum evaporator with a glow discharge unit. So
what's a tech to do?

Any suggestions as to how to fake a glow are greatly appreciated.

Please help me to glimmer, glimmer. ;-)


Unable to glow,
===================================================================
If this is the kind of treatment one would apply to carbon coated grids to
make them more hydrophilic, we can produce the same effect in the SPI Plasma
Prep II Plasma Etcher but with an "air" plasma instead of an oxygen plasma
(using oxygen would change your sample, actually it would probably etch away
and effectively destroy your sample). The Plasma Prep II unit is shown on
URL
http://www.2spi.com/catalog/instruments/etchers1.shtml

We would be happy to do a "demo" of the technique for you if you sent us
your grids in a grid box. Contact me off-line and I will give you shipping
instructions. When this technique is used to make carbon coated grids
hydrophilic, the effect seems to last something like 90 days.

Disclaimer: SPI Supplies is the manufacturer of the Plasma Prep II plasma
etcher/cleaner/asher so we would have a vested interest in seeing more of
them sold.....

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================


From daemon Thu Aug 14 15:32:41 2003



From: Pacific GridTech :      wendy-at-grid-tech.com
Date: Thu, 14 Aug 2003 13:22:45 -0700 (PDT)
Subject: Re: Carbon Support Films for TEM

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Dear Steve and other microscopist,

The Formvar on your Carbon film is hard to be cleaned
completely. The rest of Formvar could cause several
problems, such as charging, poor stigmatism, drifting
of specimen and even chemical reaction with your
sample in EM.

I suggest you to try the pure carbon film coated grid,
therefore you don't need to clean the Formvar on your
grids. This carbon film didn't touch with any plastics
while it was made. The pure carbon film is floated on
the surface of water and sit on the cleaned grids.

We do produce this kind of grid. For more information,
please check our website at

http://www.grid-tech.com/

Good luck.

Wendy
=====
Pacific GridTech
A high quality EM grid provider
3505 Caminito Carmel Landing
San Diego, CA 92130, USA
Tel: (858) 336 8938; Fax: (858) 259 5511
Email: info-at-grid-tech.com
Web: http://www.Grid-Tech.com/

--- Steve Parry {sparry-at-cmm.uwa.edu.au} wrote:
}
}
} I have recently switched to Formvar in Diethylene
} Chloride as a base
} for making carbon coated grids, because I was told
} that I would get
} bettter continuous films, the problem I am having is
} that I cannot
} get rid of the forvar after coating.
} Does anyone out there have a method for making C
} coated grids that
} works everytime?? I am getting to the end of my
} patience and have
} wasted an awful lot of grids over the last few
} months.
}
} Thanks
}
} Steve Parry
} --
} Steve Parry
} Centre for Microscopy & Microanalysis,
} (M010)
} The University of Western Australia,
} 35 Sterling Highway, Crawley,
} WA 6009, Australia.
}
} Fax: +61-8-9380-1087
} Email: sparry-at-cmm.uwa.edu.au
} Phone: +61-8-9380-8057 [24 hour voicemail
} attached]
}
}


=====
Pacific GridTech
A high quality EM grid provider
3505 Caminito Carmel Landing
San Diego, CA 92130, USA
Tel: (858) 336 8938; Fax: (858) 259 5511
Email: info-at-grid-tech.com
Web: http://www.Grid-Tech.com/


From daemon Thu Aug 14 15:33:58 2003



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 14 Aug 2003 15:28:31 -0500
Subject: Re: grid glue recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karl Garsha {garsha-at-itg.uiuc.edu} ASKED THE FOLLOWING QUESTION:

} Does anybody know a quick recipe for "grid glue" made out of scotch
} tape (or other sticky stuff) and some solvent for glueing films to
} TEM grids? Thanks in advance.
} -Karl
}
RESPONSE:

GRID GLUE can be prepared by dissolving the adhesive from 2 inches of
Scotch Brand transparent tape in 10 ml of ethylene dichloride. Please
be sure to use the transparent tape (old style) rather than the Magik
Tape since the adhesives are apparently different. I believe that
chloroform can also be used as a solvent.

It might also be posible (and I invite microscopy vendors to chime
in) to use the commercially prepared Grid-Stick Glue (EMS sells it,
for example) but it will have to be diluted.... I have not tried
this, however.

JB

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################


From daemon Thu Aug 14 15:42:53 2003



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Thu, 14 Aug 2003 16:36:59 -0400
Subject: Philips 505 SEM parts available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are getting rid of a functioning Philips 505 SEM. Anyone with a 500
series scope that might like parts let me know ASAP. SEI,BEI,CL,W-hairpin
(boxes of filaments), LAB6 (sorry no filaments - got IG pumps), pneumatic
valves, bit and pieces, etc. etc.. . .





Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Thu Aug 14 15:49:38 2003



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 14 Aug 2003 15:45:26 -0500
Subject: Re: How to simulate glow discharge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

There are several possibilities here, but none of them are as good as
the real thing.

1. Put your grids in the chamber of a sputter coater but cover the
grids with a "tent" of filter paper. The tent should completely cover
over the grids so that few of the metal particles will strike it but
the argon ions will. Alternatively, you could turn the slide
containing the grids upside down so that they face away from the
target. Just be sure to leave some space underneath, so the ions can
contact the grids. Activate the sputtering process so that you see
the plasma for 40-50 sec. You might have to experiment here so that
you minimize the presence of metal. If you have a carbon sputterer, I
believe that would be the best.

2. You might try a dozen hits of the grids with one of the
electrostatic guns (used by photographers). This sometimes does work.

3. Finally, and don't ask why this works, just leaving the grids for
several days inside of a refrigerator will cause them to become
hydrophilic. I am guessing that the trapped organics (odors, fumes,
etc.) are depositing onto the active surface of the carbon. This is
what happens when you place sodium bicarb in the refrig to trap odors.

Let us know which work best for you.

JB

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From daemon Thu Aug 14 16:26:29 2003



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 14 Aug 2003 14:55:56 -0700 (PDT)
Subject: Electroscan E3 ESEM query

Contents Retrieved from Microscopy Listserver Archives
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A couple of times I just dissolved cellotape in chloroform - as much as
could be squeezed into a small 10 ml sample bottle. Saturated solution? Not
scientific but it helped when needed!

Keith Ryan
Marine Biological Association of the UK
& University of Plymouth, UK


----- Original Message -----
} From: "Karl Garsha" {garsha-at-itg.uiuc.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, August 14, 2003 4:19 PM


Hello,
Just another question about our Electroscan E3 ESEM. Lately, we've
noticed that when you start up the main console power, the right screen
remains grey and doesn't load the microscope software such as the
vacuum/gun/image menus. The left screen has a scale bar on it, but
nothing works. I have to power down and then power up, sometimes 3x for
it to boot up correctly.

Anyone have any advice where I should look for problems?
Thanks for the help.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793


From daemon Thu Aug 14 19:29:45 2003



From: Larry Ackerman :      ackerman-at-msg.ucsf.edu
Date: Thu, 14 Aug 2003 17:09:49 -0700
Subject: Re: grid glue recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I make a general purpose glue by stuffing several feet of
Scotch Double Stick tape (other types would probably work
as well) into a 100ml bottle then adding about 50 ml of
heptane. Shake the bottle several times a day and in a few
days you'll have a nice solution that will leave a sticky
residue when it dries. This adhesive may not be the best
to use in an electron microscope since it may outgas and
contaminate your system--do some tests. Back in the 1970's
there was a paper given at the MSA (EMSA) meeting
describing a solution of Neoprene W in chloroform. It
worked well with minimal outgassing but the only source of
the Neoprene was DuPont. I have a few pieces buried
somewhere but hopefully someone else has a beter source.

On Thu, 14 Aug 2003 10:19:19 -0500
Karl Garsha {garsha-at-itg.uiuc.edu} wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America

Larry Ackerman
Advanced Imaging Lab/Dr. John Sedat/Dr. David Agard
Dept. of Biochemistry & Biophysics
University of California San Francisco
600-16th Street, Box 2140
San Francisco, CA 94143

415-514-4052 FAX 415-514-4142


From daemon Fri Aug 15 01:54:51 2003



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Fri, 15 Aug 2003 08:40:51 +0200
Subject: Re: Electroscan E3 ESEM query

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,

This might be due to a bad connection / malfunctioning of a SCSI-card
inserted in your computer to connect the computer with the
microscope. If one connection fails, ik can interfere other
connections too. Try to see how the pc starts up when you disconnect
the microscope from it. Then start up again after connecting one by
one.

Another possibility is, if you have SCSI-hardware, there are
conflicts with the startup-sequence. In other words, SCSI-hardware
should start up in a particular order (one has to start up
BEFORE/AFTER another). If you just added a new piece of hardware, the
numbering might be conflicting.

Sven Terclavers

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--


From daemon Fri Aug 15 06:53:05 2003



From: =?iso-8859-1?Q?=22Bilde-S=F8rensen=2C_J=F8rgen=22?= :      j.bilde-at-risoe.dk
Date: Fri, 15 Aug 2003 13:42:07 +0200
Subject: Electroscan E3 ESEM query

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We sometimes have the same problem with our E-3 scope. In our case it occurs because the disk drive does not begin to read the disk. In that case the yellow light on the disk drive will not light up when you start the microscope.

We have developed a special trick for our E-3 scope, where we give the disk a slight pressure to the left at the end of the insertion into the drive. When the disk has been inserted that way, it can usually be read by the drive.

Hope this helps.

{:::} --- {:::} --- {:::} --- {:::} --- {:::} --- {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



-----Original Message-----
} From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU]
Sent: 14. august 2003 23:56
To: Microscopy-at-sparc5.microscopy.com


Hello,
Just another question about our Electroscan E3 ESEM. Lately, we've
noticed that when you start up the main console power, the right screen
remains grey and doesn't load the microscope software such as the
vacuum/gun/image menus. The left screen has a scale bar on it, but
nothing works. I have to power down and then power up, sometimes 3x for
it to boot up correctly.

Anyone have any advice where I should look for problems?
Thanks for the help.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Aug 15 07:51:24 2003



From: David Elliott :      David.Elliott-at-yale.edu
Date: Fri, 15 Aug 2003 08:44:56 -0400
Subject: Re: grid glue recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use double sided tape in chloroform (a ball of tape that is just
covered by the chloroform). Shake the bottle for 20 sec. and remove
what is left of the tape (you want the glue off of the tape, not the
plastic).
Not too scientific, but it works.
David


On Thursday, August 14, 2003, at 11:19 AM, Karl Garsha wrote:

} -----------------------------------------------------------------------
} -
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} .
}
}
} Does anybody know a quick recipe for "grid glue" made out of scotch
} tape (or other sticky stuff) and some solvent for glueing films to TEM
} grids? Thanks in advance.
} -Karl
}
} --
} Karl Garsha
} Light Microscopy Specialist
} Imaging Technology Group
} Beckman Institute for Advanced Science and Technology
} University of Illinois at Urbana-Champaign
} 405 North Mathews Avenue
} Urbana, IL 61801
} Office: B650J
} Phone: 217.244.6292
} Fax: 217.244.6219
} Mobile: 217.390.1874
} www.itg.uiuc.edu
}
}
}
}
____________________

David Elliott Ph.D.

Yale University School of Medicine
Campus Address: TAC S160
333 Cedar Street
PO Box 208022
New Haven, CT   06520-8022

Tel: (203) 785-7572
Fax: (203) 785-6815



From daemon Fri Aug 15 08:34:28 2003



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 15 Aug 2003 14:28:13 +0100 (GMT Daylight Time)
Subject: Re: How to simulate glow discharge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Re method 3. placing grids in a fridge, do you put them in
on filter paper in a petri dish with the lid on, for
instance?

Dave


On Thu, 14 Aug 2003 15:45:26 -0500 "John J. Bozzola"
{bozzola-at-siu.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Paula,
}
} There are several possibilities here, but none of them are as good as
} the real thing.
}
} 1. Put your grids in the chamber of a sputter coater but cover the
} grids with a "tent" of filter paper. The tent should completely cover
} over the grids so that few of the metal particles will strike it but
} the argon ions will. Alternatively, you could turn the slide
} containing the grids upside down so that they face away from the
} target. Just be sure to leave some space underneath, so the ions can
} contact the grids. Activate the sputtering process so that you see
} the plasma for 40-50 sec. You might have to experiment here so that
} you minimize the presence of metal. If you have a carbon sputterer, I
} believe that would be the best.
}
} 2. You might try a dozen hits of the grids with one of the
} electrostatic guns (used by photographers). This sometimes does work.
}
} 3. Finally, and don't ask why this works, just leaving the grids for
} several days inside of a refrigerator will cause them to become
} hydrophilic. I am guessing that the trapped organics (odors, fumes,
} etc.) are depositing onto the active surface of the carbon. This is
} what happens when you place sodium bicarb in the refrig to trap odors.
}
} Let us know which work best for you.
}
} JB
}
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}
}
}
} This incoming email to UWE has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Aug 15 08:34:30 2003



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 15 Aug 2003 08:27:27 -0500
Subject: Re: grid glue recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We have used "grid pens" with glue with great success for our
immunocytochemistry work. Initially, we had put up with LR White and
Unicryl sections looking like a ragged piece of kleenex in the TEM.
Then we moved to formvar/carbon grids, but had severe problems with
"stickiness" causing high background labeling, apparently due to both
antibody binding and mechanical trapping of the gold conjugate, not to
mention various other folding and layering artifacts. Finally, after a
suggestion from someone on this list, we tried the pens. Now our
sections remain largely intact, the background problem is under control,
and we are much happier campers. We do not dilute the glue in the pens,
but just put one dab on grids on filter paper and let them dry a couple
minutes before picking up our sections.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core---We do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/



-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Thursday, August 14, 2003 3:29 PM
To: Microscopy-at-sparc5.microscopy.com


Karl Garsha {garsha-at-itg.uiuc.edu} ASKED THE FOLLOWING QUESTION:

} Does anybody know a quick recipe for "grid glue" made out of scotch
} tape (or other sticky stuff) and some solvent for glueing films to
} TEM grids? Thanks in advance.
} -Karl
}
RESPONSE:

GRID GLUE can be prepared by dissolving the adhesive from 2 inches of
Scotch Brand transparent tape in 10 ml of ethylene dichloride. Please
be sure to use the transparent tape (old style) rather than the Magik
Tape since the adhesives are apparently different. I believe that
chloroform can also be used as a solvent.

It might also be posible (and I invite microscopy vendors to chime
in) to use the commercially prepared Grid-Stick Glue (EMS sells it,
for example) but it will have to be diluted.... I have not tried
this, however.

JB

##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################



From daemon Fri Aug 15 10:05:04 2003



From: paul r hazelton, PhD :      paul_hazelton-at-umanitoba.ca
Date: Fri, 15 Aug 2003 09:55:51 -0500
Subject: Re: How to simulate glow discharge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


paula

first, have you tried a standard negative stain preparation? a lot of
the time there is enough protein present in the sample to overcome the
hydrophobicity. just leave the sample on the grid for about a minute.

second, try an airfuge, EM-90 preparation. the combination of
centrifugal force for 1/2 hr plus protein present in the preparation
usually overcomes the need for glow discharge.

third, try pre-treating the grids with either poly-L-lysine or Alcian
blue. use a 1% solution, float the grids on the solution for 1-2
minutes, wick off the excess, then let dry

one of these should work

paul


Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926



From daemon Fri Aug 15 11:00:45 2003



From: Mayandi Sivaguru :      sivagurum-at-missouri.edu
Date: Fri, 15 Aug 2003 09:54:38 -0500
Subject: Re: TIRF evaluation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael, The 1.65 NA objective is not absolutely necessary, but as you
aware TIRF needs higher NA (NA} n). But you can obtain fairly good images
with 1.45 NA lenses available now with Nikon and Zeiss. The 1.65 NA
available only with Olympus as of now and you may need to pay a higher
price for that.
I had an introductory practical course experience only with Olympus system
with both 1.45 and 1.65 lenses.
If you need any other information please let me know by email.

Shiv

Mayandi Sivaguru, Ph.D.,
Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2, Tucker Hall
University of Missouri
Columbia, MO 65211-7400
sivagurum-at-missouri.edu

Voice: 1-573-882-4895
Fax: 1-573-882-0123

www.biotech.missouri.edu/mcc/



At 12:42 PM 8/14/03 -0400, you wrote:
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri Aug 15 17:04:55 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 15 Aug 2003 14:56:12 -0700
Subject: Re: How to simulate glow discharge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It came to my mind, that exposure of the grids to the strong UV (real UV)
light do the trick also. Effect will vary depends from the UV source and
intensity. As I remember, grids are good for about 40 min after all.
Personally, I prefer to use poly-lysine treatment. In my hands it works
nearly the same as glow discharge. Even better, because it does not made
surface rough. Sergey

At 01:45 PM 8/14/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Aug 15 19:24:02 2003



From: sswaffe-at-abv.bg (by way of Ask-A-Microscopist)
Date: Fri, 15 Aug 2003 19:14:28 -0500
Subject: Ask-A-Microscopist: permanent slides for examination under

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sswaffe-at-abv.bg) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Wednesday, August 13, 2003 at 02:56:55
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: 54 "st.Ivan Rilski"

Education: 6-8th Grade Middle School

Location: Sofia Bulgaria

Question: How can I prepare permanent slides for examination under microscope?What materials should I use for preserving the speciment for a very long time?

---------------------------------------------------------------------------


From daemon Sat Aug 16 14:20:14 2003



From: Richard Gardiner :      rbgardiner-at-rogers.com
Date: Sat, 16 Aug 2003 15:08:59 -0400
Subject: EM Textbooks Used

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:

I was wondering what text books people are using to teach either
Undergraduate or Graduate courses in Biological TEM.

Richard
Gardiner



From daemon Sun Aug 17 21:37:27 2003



From: Microshaw-at-aol.com
Date: Sun, 17 Aug 2003 22:18:45 -0400
Subject: Re: Ask-A-Microscopist: permanent slides for examination under microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Veselin-
There are commercial preparations you can buy, but you can also use clear nail polish if you do not have access to these cements. But first- you must remove the water from your specimen, by soaking in alcohol. First water plus alcohol, then gradually reducing the water content. Making permanent slides takes a different process depending upon what the specimen is. What are you mounting on the slide?
Rgds,
Mike Shaw
}
} Email: sswaffe-at-abv.bg
} Name: Veselin Andreev
}
} Organization: 54 "st.Ivan Rilski"
}
} Education: 6-8th Grade Middle School
}
} Location: Sofia Bulgaria
}
} Question: How can I prepare permanent slides for examination under microscope?What materials should I use for preserving the speciment for a very long time?
}
} ------------------------------------------------------------
} ---------------


From daemon Mon Aug 18 08:12:46 2003



From: Anthony Greco :      tgreco-at-seas.marine.usf.edu
Date: Mon, 18 Aug 2003 09:05:16 -0400
Subject: TEM textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard
I use Electron Microscopy, 2nd edition by John Bozzola and Lonnie
Russell for my graduate TEM course. It is an excellent book and my
students really enjoy it, especially the survey of biological
ultrastructure at the back.

Tony Greco



From daemon Mon Aug 18 09:07:57 2003



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 18 Aug 2003 10:06:37 -0400
Subject: Re: EM Textbooks Used

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My favorite is "A beginners handbook in Biological Transmission Electron
Microscopy", by Brenda S. Weakley, Chruchill Livingstone is the
publisher. My second edition is 1981, there may be a more recent
edition. "Biological Techinques in Electron Microscopy" by Clinton Dawes
is also good but my copy is so old (1971) it may be out of print.

Geoff

Richard Gardiner wrote:

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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Mon Aug 18 09:10:38 2003



From: Philip Koeck :      Philip.Koeck-at-biosci.ki.se
Date: Mon, 18 Aug 2003 16:07:36 +0200
Subject: Re: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can easily use the method described by Mike to make
about 50 pure carbon grids at a time (much cheaper than
buying if you already have the equipment).

You place about 50 grids on a square piece of filter paper
lying on a little "table" made of wire mesh under the water surface.
To get the grids under water make them wet first and push them
through the surface vertically.
Then you lower the water surface by removing water from underneath
(for example with a syringe or a plastic tube with a valve) to place
the carbon film (floated off the mica) on top of the grids.
(Of course the carbon film has to be in one piece in this case.)
Push the carbon into position with something blunt (a paddle ? :)

Philip Koeck
Svdertvrns Hvgskola and
Karolinska Institutet
Dept. of Bioscience at Novum
S-14157 Huddinge
Sweden
phone: +46-8-6089186
fax: +46-8-6089290
http://www.biosci.ki.se/em
_______________________________________

} } Steve,
} } When I want just carbon as a substrate I will
} } coat freshly cleaved mica with carbon in a metal
} } evaporator. This allows me to dictate the carbon
} } thickness and I have no trouble floating off grid
} } size squares(prescored)in a drop of water. Picking up the
} } carbon with glow discharged grids (flat side)
} } is a snap. Make sure everything is clean.
} } This should give you thin clean substrates.
} } Mike D.




From daemon Mon Aug 18 10:44:15 2003



From: Douglas Bray :      bray-at-uleth.ca
Date: Mon, 18 Aug 2003 09:38:49 -0600
Subject: Re: EM Textbooks Used

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've used the Bozzola and Russell textbook entitled "Electron Microscopy:
Principles and Techniques for Electron Microscopy" since the first edition was
published in 1992. A second edition was published in 1999. It was especially
suited to my needs as I taught both an SEM and a TEM course each year - primarily
to undergraduate students. The students were able to use the same text for both
courses. The authors provide a good general background on theory, and an
excellent treatment of procedures and techniques for both types of microscopy.
Although there may be other texts out there that are suitable as well, I highly
recommend this one as a text to teach from.

Doug Bray

Richard Gardiner wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello:
}
} I was wondering what text books people are using to teach either
} Undergraduate or Graduate courses in Biological TEM.
}
} Richard
} Gardiner



From daemon Mon Aug 18 11:16:58 2003



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 18 Aug 2003 11:12:33 -0500
Subject: Phosphotungstic acid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need a protocol for staining collagen thin sections with
phosphotungstic acid.

Thanks a lot,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy




From daemon Mon Aug 18 11:35:05 2003



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Mon, 18 Aug 2003 09:31:31 -0700
Subject: RE: EM Textbooks Used

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bozzola, John J., Russell, Lonie D. - Electron Microscopy - Principles and Techniques for Biologists.

Alice Dohnalkova
Environmental Microbiology
Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692 office
(509) 376-3654 TEM lab
fax (509) 376-1321


} -----Original Message-----
} From: Richard Gardiner [SMTP:rbgardiner-at-rogers.com]
} Sent: Saturday, August 16, 2003 12:09 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: EM Textbooks Used
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello:
}
} I was wondering what text books people are using to teach either
} Undergraduate or Graduate courses in Biological TEM.
}
} Richard
} Gardiner
}
}


From daemon Mon Aug 18 12:25:35 2003



From: Robert A Underwood :      underwoo-at-u.washington.edu
Date: Mon, 18 Aug 2003 10:18:36 -0700 (PDT)
Subject: Re: Phosphotungstic acid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,
We use PTA to stain thin sections of human skin for collagen. I make up a
fresh solution of 1% PTA in dH20. Mix thoroughly and filter twice through a
#1 Whatman. Leave the pH acid. Don't adjust it toward neutral (it won't
stain as well). Stain sections 30 mimutes. staining sequence should be:
1) PTA
2) UA
3) PB

Robert Underwood
Dermatology Research Center
University of Washington


On Mon, 18 Aug 2003, Dusevich, Vladimir wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I need a protocol for staining collagen thin sections with
} phosphotungstic acid.
}
} Thanks a lot,
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}
}




From daemon Mon Aug 18 13:42:10 2003



From: Barbara Foster :      bfoster-at-mme1.com
Date: Mon, 18 Aug 2003 14:28:19 -0700
Subject: Pittsburgh Conference Memorial National College Grants Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With many of you looking for extra money for microscopy labs and the recent convergence of microscopy and spectroscopy, I thought this small grant program might be of interest.

Good hunting!
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suite 102
Springfield, MA 01118
PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com

^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^
Need class-room sized quantities of Optimizing Light Microscopy? Call us today.
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^



ANNOUNCEMENT

2004 Pittsburgh Conference Memorial National
College Grant Program Eligibility Criteria

The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy (a Pennsylvania non-profit
Corporation) and its co-sponsoring technical societies, The Society for Analytical Chemists of Pittsburgh (SACP) and The Spectroscopy Society of Pittsburgh (SSP) proudly announce the 2004 Pittsburgh Conference Memorial National College Grants (PCMNCG) Program.

Grants will be awarded to small college science departments for the purchase of scientific equipment, audio-visual or other teaching aids, and/or library materials for use in the teaching of science at the undergraduate level.

Based on submitted proposals, at least twelve (12) colleges will be selected to receive grants, each grant having a maximum of $9,000.

To be eligible for an award, schools must meet the following criteria.
1. Enrollment must not exceed 5000 full-time students.

2. No more than 25% of the operating budget may come from national or state governments.
NOTES
Two-year community colleges sponsored by political subdivisions of a state are not bound by criteria 1 and 2.

Schools should not expect funding from other programs sponsored by SACP and SSP (if they receive a PCMNCG grant).
3. Requests that support undergraduate research are allowed. However, requests for equipment to be used solely for non-instructional research purposes shall not be funded.

4. Awards may be used as part of “Matching Grant” programs; use of matching funds to increase the grant’s impact is encouraged.

5. Previous awardee colleges are ineligible for the PCMNCG program for a three-year period following receipt of the grant (awardee colleges from 2001, 2002 and 2003 are not eligible for the 2004 program).

6. Grant applications and proposals must be received no later than December 1, 2003.
Faculty members may obtain application forms and additional information from:

Rita M. Windisch, Ph.D.
The Pittsburgh Conference PCMNCG
300 Penn Center Blvd., Suite 332
Pittsburgh, PA 15235-5503
Telephone (412) 825-3220, Ext. 204
FAX (412) 825-3224
E-mail: windisch-at-pittcon.org

or from the Pittsburgh Conference Website: www.pittcon.org
Announcement of the awards will be made by February 2004. Schools chosen will join the list of over 200 institutions honored since the inception of this program in 1974.

H:\PCMNCG\2004\Announcement for Editors.doc




From daemon Mon Aug 18 14:37:04 2003



From: Jonathan McGovern :      semrus-at-shaw.ca
Date: Mon, 18 Aug 2003 13:27:01 -0600
Subject: SEM - Uses sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All;

We are shopping for a good used sputter coater complete with a carbon fiber
evaporation attachment.
Please contact me off line.

Cheers;
Jon McGovern
J.P. McGovern and Associates
semrus-at-shaw.ca




From daemon Mon Aug 18 14:37:50 2003



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Mon, 18 Aug 2003 14:33:56 -0500
Subject: Re: EM Textbooks Used

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think Dawes is still available from Ladd Research.
But I prefer Bozzola and Russell for a text, and Maunsbach and
Afzelius "Biomedical Electron Micropscopy" for lots of EMs comparing
fixatives, buffers, embedding media, and so forth.

Phil

} My favorite is "A beginners handbook in Biological Transmission
} Electron Microscopy", by Brenda S. Weakley, Chruchill Livingstone
} is the publisher. My second edition is 1981, there may be a more
} recent edition. "Biological Techinques in Electron Microscopy" by
} Clinton Dawes is also good but my copy is so old (1971) it may be
} out of print.
}
} Geoff
}
} Richard Gardiner wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Mon Aug 18 14:48:44 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 18 Aug 2003 13:06:15 -0700
Subject: Re: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Phillip
Your way to mount carbon on the grids has one very serious disadvantage:
when you mount carbon this way, you will use "air-side" of carbon as a
working surface. This side is usually more grainy and dirty. The whole
idea of using carbon from mica is to use "mica-side" of carbon as a working
surface. The idea here is that the carbon face toward mica is more uniform
and perfectly clean (it was not exposured to the air). In order to use
"good" side of carbon, you have to put grids on floated carbon and then
pick the grids with Parafilm for instance. Similarly, you need to do the
same for plastic film created on water - working side is "water-side", not
air. Only one exception I do know is Formvar film created on the
glass. Glass is not such clean and perfect as mica or water surface,
therefore, you need to use "air-side". Then your technique will work
perfectly. I also use your technique to mount holey film on the grids.
Best wishes, Sergey.


At 04:07 PM 8/18/03 +0200, you wrote:
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Aug 18 18:14:17 2003



From: ssamuelsson-at-eyetk.com (by way of Ask-A-Microscopist)
Date: Mon, 18 Aug 2003 17:59:25 -0500
Subject: Ask-A-Microscopist : Whole Mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ssamuelsson-at-eyetk.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, August 18, 2003 at 14:09:26
---------------------------------------------------------------------------

Email: ssamuelsson-at-eyetk.com
Name: Steve Samuelsson

Organization: Eyetech Pharmaceuticals

Title-Subject: Whole Mounts

Question: Regarding growing endothelial cells on nickel grids for whole mount TEM analysis: our luck is inconsistent and we now need to dig further for the reason. 200 and 300 mesh nickel grids are coated with ~0.5% formvar in ethylene dichloride (we also tried chloroform) and picked up on a 13mm cover slip. These are allowed to dry and age a day or longer. Prior to plating, the slips are UV sterilized 10-15', incubated in 1% gelatin ~45', washed 1x with PBS, culture medium added followed by cells. Our issue is incomplete (or downright lousy) growth of cells on the grids. Cells are robust and growing fine on the bottom of the 24 well plate so we suspect something with the grids. My first thought was that we needed carbon coating of the grids but have some trouble with this explanation. Second thought was that the solvent was still coming out of the films and poisoning the cells; thus the 'aging' issue with the cover slips. Any suggestions?


---------------------------------------------------------------------------


From daemon Mon Aug 18 18:14:18 2003



From: hafeez189-at-yahoo.com (by way of Ask-A-Microscopist)
Date: Mon, 18 Aug 2003 18:00:02 -0500
Subject: Ask-A-Microscopist: optical polarization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (hafeez189-at-yahoo.com) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Monday, August 18, 2003 at 14:38:56
---------------------------------------------------------------------------

Email: hafeez189-at-yahoo.com
Name: Hafeez Anwar

Organization: University of Agriculture, Faisalabad, Pakistan

Education: Graduate College

Location: Pakistan

Question: hi, i am working on optical polarization with hot stage and i am studying the morphology of polymer blends.
my question is that why the colors of the sample change when we rotate the analyzer ?(kindly ,explain in detail)


---------------------------------------------------------------------------


From daemon Mon Aug 18 18:53:43 2003



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Mon, 18 Aug 2003 18:48:42 -0500
Subject: Staining toughened nylon

Contents Retrieved from Microscopy Listserver Archives
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Listers,

A user has the following question. Please reply off line to her at:
DCOLLISO-at-glcc.com (Donna Collison)


Does anyone have experience in staining 'toughened nylon' for backscattered
compositional analysis. Toughened nylon as we are describing it is nylon
having butadiene rubber phases within the nylon matrix. We need to
determine were an antimony component is within the formulation. So far we
have placed fractured pieces of this material directly in osmium tetroxide
for a 24 hour period and was not able to detect any contrast. Any
suggestions and help would be greatly appreciated.

Many thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907



From daemon Mon Aug 18 18:57:33 2003



From: Rick A. Harris :      raharris-at-ucdavis.edu
Date: Mon, 18 Aug 2003 16:52:52 -0700
Subject: Salmonella in the SEM

Contents Retrieved from Microscopy Listserver Archives
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I need a little advice on safely examining plants infected with Salmonella
sp. I have a user who wants to use cryofixation of melon and tomato plants
that have been inoculated with the Salmonella sp. bacterium. She would like
to bring the inoculated plants into the EM facility, dissect a small bit of
the plant surface and then do a conventional cryo prep and examine in the
SEM. I am not familiar with the safest method of examining pathogens in the
SEM. Can the bacteria survive cryo fixation? What is the best method of
cleaning the scope after use? What about the instruments used for
dissection? As you can see, I have a lot of questions and before we proceed
with this project I need to satisfy myself that we are doing this correctly.

Thanks for you help.

Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
http://microscopy.mcb.ucdavis.edu
raharris-at-ucdavis.edu


From daemon Tue Aug 19 02:44:56 2003



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 19 Aug 2003 08:36:38 +0100 (GMT Daylight Time)
Subject: Re: Ask-A-Microscopist : Whole Mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could the nickel be toxic? Why not try gold?

Dave


On Mon, 18 Aug 2003 17:59:25 -0500 by way of
Ask-A-Microscopist {ssamuelsson-at-eyetk.com} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ssamuelsson-at-eyetk.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, August 18, 2003 at 14:09:26
} ---------------------------------------------------------------------------
}
} Email: ssamuelsson-at-eyetk.com
} Name: Steve Samuelsson
}
} Organization: Eyetech Pharmaceuticals
}
} Title-Subject: Whole Mounts
}
} Question: Regarding growing endothelial cells on nickel grids for whole mount TEM analysis: our luck is inconsistent and we now need to dig further for the reason. 200 and 300 mesh nickel grids are coated with ~0.5% formvar in ethylene dichloride (we also tried chloroform) and picked up on a 13mm cover slip. These are allowed to dry and age a day or longer. Prior to plating, the slips are UV sterilized 10-15', incubated in 1% gelatin ~45', washed 1x with PBS, culture medium added followed by cells. Our issue is incomplete (or downright lousy) growth of cells on the grids. Cells are robust and growing fine on the bottom of the 24 well plate so we suspect something with the grids. My first thought was that we needed carbon coating of the grids but have some trouble with this explanation. Second thought was that the solvent was still coming out of the films and poisoning the cells; thus the 'aging' issue with the cover slips. Any suggestions?
}
}
} ---------------------------------------------------------------------------
}
}
}
} This incoming email to UWE has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Aug 19 06:40:26 2003



From: rcmoretz-at-att.net
Date: Tue, 19 Aug 2003 11:32:12 +0000
Subject: Re: Ask-A-Microscopist : Whole Mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My own experience has been that only gold or titanium grids give consistent
results with this procedure. Both copper and nickel tend to be quite toxic.

Roger Moretz, Ph.D.
Dept of Toxicology
BI Pharmaceuticals
Ridgefield, CT

--
Where the world is only slightly
less weird than it actually is.
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}
} Could the nickel be toxic? Why not try gold?
}
} Dave
}
}
} On Mon, 18 Aug 2003 17:59:25 -0500 by way of
} Ask-A-Microscopist {ssamuelsson-at-eyetk.com} wrote:
}
} } ------------------------------------------------------------------------
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} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was submitted
} by (ssamuelsson-at-eyetk.com) from
} http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
} August 18, 2003 at 14:09:26
} } ---------------------------------------------------------------------------
} }
} } Email: ssamuelsson-at-eyetk.com
} } Name: Steve Samuelsson
} }
} } Organization: Eyetech Pharmaceuticals
} }
} } Title-Subject: Whole Mounts
} }
} } Question: Regarding growing endothelial cells on nickel grids for whole mount
} TEM analysis: our luck is inconsistent and we now need to dig further for the
} reason. 200 and 300 mesh nickel grids are coated with ~0.5% formvar in ethylene
} dichloride (we also tried chloroform) and picked up on a 13mm cover slip. These
} are allowed to dry and age a day or longer. Prior to plating, the slips are UV
} sterilized 10-15', incubated in 1% gelatin ~45', washed 1x with PBS, culture
} medium added followed by cells. Our issue is incomplete (or downright lousy)
} growth of cells on the grids. Cells are robust and growing fine on the bottom
} of the 24 well plate so we suspect something with the grids. My first thought
} was that we needed carbon coating of the grids but have some trouble with this
} explanation. Second thought was that the solvent was still coming out of the
} films and poisoning the cells; thus the 'aging' issue with the cover slips. Any
} suggestions?
} }
} }
} } ---------------------------------------------------------------------------
} }
} }
} }
} } This incoming email to UWE has been independently scanned for viruses and any
} virus detected has been removed using McAfee anti-virus software
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}


From daemon Tue Aug 19 09:04:56 2003



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 19 Aug 2003 14:57:17 +0100 (GMT Daylight Time)
Subject: Re: Salmonella in the SEM

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Suppliers are, in my experience, reluctant to comment on
the problem potential hazards of non chemically fixed
microorganisms in cryoSEM orlow vacuum/ESEM.

I have seen a paper showing that some bacteria (which
species?) survive cryoSEM sessions. In theory they could
get pumped into your rotary pump and out of the room
(comments anyone?).

A colleague looked for presence of bacteria in the
chamber after ESEM and found little, I think. (I will
e-mail her for comment).

Here if a colleague can show the bacteria are harmless we
would consider looking at them unfixed. If they are
pathogenic I insist they are fixed first.

Your colleague should advise on cleaning tools. One of our
users cleaned forceps in 90% ethanol. You can wipe over
the stage and chamber but what about the column?

I hope you have started an interesting debate on a topic we
mull over here periodically.

Dave



On Mon, 18 Aug 2003 16:52:52 -0700 "Rick A. Harris"
{raharris-at-ucdavis.edu} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I need a little advice on safely examining plants infected with Salmonella
} sp. I have a user who wants to use cryofixation of melon and tomato plants
} that have been inoculated with the Salmonella sp. bacterium. She would like
} to bring the inoculated plants into the EM facility, dissect a small bit of
} the plant surface and then do a conventional cryo prep and examine in the
} SEM. I am not familiar with the safest method of examining pathogens in the
} SEM. Can the bacteria survive cryo fixation? What is the best method of
} cleaning the scope after use? What about the instruments used for
} dissection? As you can see, I have a lot of questions and before we proceed
} with this project I need to satisfy myself that we are doing this correctly.
}
} Thanks for you help.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} http://microscopy.mcb.ucdavis.edu
} raharris-at-ucdavis.edu
}
}
}
} This incoming email to UWE has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Aug 19 10:11:47 2003



From: sghoshro-at-NMSU.Edu
Date: Tue, 19 Aug 2003 09:04:44 -0600 (MDT)
Subject: Re: EM Textbooks Used

Contents Retrieved from Microscopy Listserver Archives
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Richard,

I highly recommend Bozzola & Russell's Electron Microscopy 2nd ed. I use
it for my graduate level EM course.

Cheers,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://www.cs.nmsu.edu/~biology/Faculty%20&%20Staff/Ghoshroy/Ghoshroy.htm
http://confocal.nmsu.edu/eml

On Sat, 16 Aug 2003, Richard Gardiner wrote:

} ------------------------------------------------------------------------
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}
}
} Hello:
}
} I was wondering what text books people are using to teach either
} Undergraduate or Graduate courses in Biological TEM.
}
} Richard
} Gardiner
}
}
}


From daemon Tue Aug 19 11:33:25 2003



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Tue, 19 Aug 2003 11:25:28 -0500
Subject: Humidity and Plastic Problems

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I have a lot of trouble making too Epon/Araldite in this weather of high
humidity. Although our hospital is air conditioned, it still seems to leave
a lot of humidity in the air [read: it's almost useless], and it seems that
we always having a lot of trouble infiltrating and getting the thin sections
to stay together in this sort of weather. Our thin sections break either in
the boat when we are cutting, or rapidly under the beam of the microscope.

Is there any way of modifying the formula of our plastic to compensate for
this problem?

This is the formula that we presently use to make our plastic:

Araldite: 65 grams
Epon [JEMBED 812 Epon relacement]: 81 grams
DDSA: 232 grams
DMP-30: 7.2 grams.


Garry Burgess
Charge Technologist
Department of Pathology - EM Laboratory
Health Sciences Centre
Winnipeg


From daemon Tue Aug 19 13:00:48 2003



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 19 Aug 2003 11:05:28 -0700
Subject: Re: Salmonella in the SEM

Contents Retrieved from Microscopy Listserver Archives
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On Monday, August 18, 2003, at 04:52 PM, Rick A. Harris wrote:

} I need a little advice on safely examining plants infected with
} Salmonella
} sp. I have a user who wants to use cryofixation of melon and tomato
} plants
} that have been inoculated with the Salmonella sp. bacterium. She
} would like
} to bring the inoculated plants into the EM facility, dissect a small
} bit of
} the plant surface and then do a conventional cryo prep and examine in
} the
} SEM. I am not familiar with the safest method of examining pathogens
} in the
} SEM. Can the bacteria survive cryo fixation? What is the best method
} of
} cleaning the scope after use? What about the instruments used for
} dissection? As you can see, I have a lot of questions and before we
} proceed
} with this project I need to satisfy myself that we are doing this
} correctly.
}
Dear Rick,
We do some cryo-TEM on (mild) pathogens, and our
safety-office-approved protocol is to do the preparation--growth and
freezing--in a biosafety hood. We are allowed to transfer the grids,
which are kept under LN2, to the cryostage as with any other specimen,
then the grids are dropped into bleach after examination. You should
assume that Salmonella will survive cryofixation, and treat your
specimens, tools, stubs, etc. as potential sources of infection. Check
with your safety office for the best sterilization method for
Salmonella, but bleach or detergent will usually disrupt bacteria. I'm
not at all certain what would be best for the inside of the SEM, but,
if your specimens remain frozen for the duration of their time inside
the scope, you may be able to avoid putting bleach or detergent into
your instrument. Good luck.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From daemon Tue Aug 19 13:14:20 2003



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 19 Aug 2003 11:22:06 -0700
Subject: Re: Ask-A-Microscopist: optical polarization

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On Monday, August 18, 2003, at 04:00 PM, by way of Ask-A-Microscopist
wrote:

} Question: hi, i am working on optical polarization with hot stage and
} i am studying the morphology of polymer blends.
} my question is that why the colors of the sample change when we rotate
} the analyzer ?(kindly ,explain in detail)
}
Dear Hafeez,
Your sample causes a rotation of the polarization vector of the light
passing through it. The amount of the rotation is proportional to the
thickness of the sample, and it will vary with the wavelength of the
light. The phenomenon is called "optical rotary dispersion" (if I
remember my optics correctly), and the details should be available in a
physics text. Consider a simple case. If you are looking through a
slab of material with uniform thickness, the light entering the sample
will be polarized, say, at 12 o'clock. As it passes through the slab,
the polarization of each wavelength is rotated to a different extent,
say, red to 5 o'clock, green to 6 o'clock, and blue to 7 o'clock. When
the analyzer is oriented at 5 o'clock, the sample will appear red, etc.
If the sample thickness varies, the apparent color at a given analyzer
position will vary with thickness, and, as the analyzer orientation
changes, the colors will shift. If your specimen is wedge-shaped, you
should see the colors in the same order as in a rainbow--assuming that
the dispersion is monotonic with wavelength, as it usually is--and the
colors will move pretty much in unison along the slope of the wedge as
the analyzer is rotated.
Yours,
Bill Tivol
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From daemon Tue Aug 19 16:41:40 2003



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 19 Aug 2003 17:03:09 -0400
Subject: Re: Humidity and Plastic Problems

Contents Retrieved from Microscopy Listserver Archives
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Dear Garry:

Epon is somewhat miscible with water, a colleague regularly goes
from 95% to an Epon:alcohol mix, so humidity may not be the problem.
First I would try all fresh plastics, Epon, Araldite and especially
DMP-30 which has a short shelf life. You could also try longer times (or
smaller pieces of tissue) and agitation to be sure infiltration is not
the problem. It could be that your plastics have gotten 'tired' just as
the weather has become more humid?

Geoff

Garry Burgess wrote:

} I have a lot of trouble making too Epon/Araldite in this weather of high
} humidity. Although our hospital is air conditioned, it still seems to leave
} a lot of humidity in the air [read: it's almost useless], and it seems that
} we always having a lot of trouble infiltrating and getting the thin sections
} to stay together in this sort of weather. Our thin sections break either in
} the boat when we are cutting, or rapidly under the beam of the microscope.
}
} Is there any way of modifying the formula of our plastic to compensate for
} this problem?
}
} This is the formula that we presently use to make our plastic:
}
} Araldite: 65 grams
} Epon [JEMBED 812 Epon relacement]: 81 grams
} DDSA: 232 grams
} DMP-30: 7.2 grams.
}
}
} Garry Burgess
} Charge Technologist
} Department of Pathology - EM Laboratory
} Health Sciences Centre
} Winnipeg
}
}
}

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Aug 19 17:27:24 2003



From: Tamara Howard :      thoward-at-unm.edu
Date: Tue, 19 Aug 2003 16:22:54 -0600 (MDT)
Subject: Re: Humidity and Plastic Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One thing I've found that at least helps is to pre-cook the embedding
molds to sort of dry them out. Also, store the resin components under
vacuum. Don't know about changing your recipe....

Good luck!

Tamara

On Tue, 19 Aug 2003, Garry Burgess wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a lot of trouble making too Epon/Araldite in this weather of high
} humidity. Although our hospital is air conditioned, it still seems to leave
} a lot of humidity in the air [read: it's almost useless], and it seems that
} we always having a lot of trouble infiltrating and getting the thin sections
} to stay together in this sort of weather. Our thin sections break either in
} the boat when we are cutting, or rapidly under the beam of the microscope.
}
} Is there any way of modifying the formula of our plastic to compensate for
} this problem?
}
} This is the formula that we presently use to make our plastic:
}
} Araldite: 65 grams
} Epon [JEMBED 812 Epon relacement]: 81 grams
} DDSA: 232 grams
} DMP-30: 7.2 grams.
}
}
} Garry Burgess
} Charge Technologist
} Department of Pathology - EM Laboratory
} Health Sciences Centre
} Winnipeg
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Tue Aug 19 18:44:42 2003



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 19 Aug 2003 16:33:17 -0700
Subject: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
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Now that the safety nazis have forced us to wear face shields, smocks and gloves for handling liquid nitrogen, they want us to wear safety glasses while doing wedge polishing, which entails frequent looking through a microscope to judge the progress. The concern is that some polish slurry 'could' splash into the eyes of a technician. Of course the safety glasses really get in the way when looking through a microscope, and our technicians are really not happy about this. I would like to hear as many opinions as possible on whether there is significant likelyhood of such a splash, and if polishing slurry, like Siton or Glanzox really poses a hazard to the eyes of a person using it. Thanks.

John Mardinly
Intel



From daemon Tue Aug 19 20:35:17 2003



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Tue, 19 Aug 2003 18:13:16 -0700
Subject: Philips 430 Available for Purchase

Contents Retrieved from Microscopy Listserver Archives
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We have a Philips 430 TEM that is available for purchase at a reasonable
price. Please contact me off line if you are interested.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Tue Aug 19 21:19:04 2003



From: Christopher S. Zurenko :      czurenko-at-umich.edu
Date: Tue, 19 Aug 2003 22:14:01 -0400 (EDT)
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
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This is a simple matter of your company doing risk assessment and covering
their butts to prevent law suits and higher insurance premiums. I don't
think it matters if it is silicon polish, rock chips off a jack hammer or
any other flying material, a company/institution wants to lessen liability
and increase safety. By telling you and your techs that you MUST wear
these items, they have less liability if someone does get something in
their eye.

Example A: Occupational safety does not say to wear the eye shields and
Technician A gets abrasive slurry compound in his/her eye. Depending on
how that compound affects the eye, he/she is now getting paid comp/injury
leave and possibly preparing a lawsuit against the company for not
providing a safe work place while OSHA is looking for answers and handing
out fines as well.

Example B: Occupational safety says to wear the eye shields and
Technician A does not because its an "inconvenience". Technician A gets
compound in his/her eye. The company can now say, "Sorry but you were
negligent in following proper protocol." The company's management and
insurance company can rest easy knowing that any lawsuit has little to no
validity due to the negligence of the employee.

I doubt there is much you can do about a perceived safety threat. If you
can state that productivity is being hampered due to the implementation of
these new safety protocols, management will probably listen to your
complaint. What do the directions and MSDSs say for these materials? Do
the manufacturers suggest wearing eye shields? I hear Oakley safety spec
glasses are expensive but comfortable.

Probably not what you wanted to hear but, for the most part, it's the hard
truth. Whether these materials pose a real threat or not, occupational
safety views it as a risk and you have to follow the rules. Allowing
anyone you supervise to take their glasses off would put your position in
jeopardy. Just something to think about.

Chris Zurenko
Univ. of Michigan
KHRI/Otopathology




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Now that the safety nazis have forced us to wear face shields, smocks
} and gloves for handling liquid nitrogen, they want us to wear safety
} glasses while doing wedge polishing, which entails frequent looking
} through a microscope to judge the progress. The concern is that some
} polish slurry 'could' splash into the eyes of a technician. Of course
} the safety glasses really get in the way when looking through a
} microscope, and our technicians are really not happy about this. I would
} like to hear as many opinions as possible on whether there is
} significant likelyhood of such a splash, and if polishing slurry, like
} Siton or Glanzox really poses a hazard to the eyes of a person using it.
} Thanks.
}
} John Mardinly
} Intel





From daemon Wed Aug 20 02:42:17 2003



From: Angela Welford :      awelford-at-salud.unm.edu
Date: Wed, 20 Aug 2003 09:58:49 -0600
Subject: RE: Humidity and plastic problems

Contents Retrieved from Microscopy Listserver Archives
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I follow your logic, but it is logic I will resist to the day I retire
(which may just have come a little closer!). Possible outcomes for
this line of thinking are that many legitimate research activities
will effectively be denied us, and more worryingly that this approach
to safety is a way for comapnies to deny their responsibilities to
employees for their safety. Would you want to work for an employer
whose rules effectively abolished any accident insurance. Just
something to think about.
Chris

Dr. Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility

----- Original Message -----
} From: "Christopher S. Zurenko" {czurenko-at-umich.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 20, 2003 3:14 AM


In high humidity Michigan, I routinely used an Epon-Araldite receipe
from Palevitz and Newcombe (JCB 45(2):383, 1970) that used 40ml DDSA,
20ml Epon (or replacement), 20ml Araldite 6005 and 1.44ml DMP-30. I
used a glass syringe for dispensing the DMP-30, a glass rod to stir the
resin, de-gassed the resin before use and between resin changes, and
kept all components under vacuum. When my resin started behaving oddly
I tossed all components and started with fresh.

Good luck!

Angela Welford, EMT
Dept of Pathology
University of New Mexico
Albuquerque, NM, 87131
505-272-1445



From daemon Wed Aug 20 11:57:24 2003



From: msteglic-at-mdanderson.org
Date: Wed, 20 Aug 2003 12:33:04 -0500
Subject: RE:Humidity and Plastic Problems

Contents Retrieved from Microscopy Listserver Archives
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The formula should not be changed. We would suggest storing the chemicals in
a desiccator cabinet with a desiccant such as silica gel..

JD Arnott

Disclaimer: Ladd Research sells the chemicals and supplies mentioned in this
e-mail.

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, August 19, 2003 12:25 PM




Garry:
I have been using an Epon/Araldite mixture here in Houston for at least the past
15 years with no problems. I doubt that you are any more hot and humid than I am
here. I do not measure mine by weight but by volume. I use the following
formula:
12 ml of Epon 812 (any substitute 812)
10 ml Araldite 502
24 ml DDSA
1 ml DMP 30

I do all of my measurements using a disposable plastic syringe. I remove the
plunger and pour in an amount just over what I need (be sure to keep the tip
cover on or it will flow out while you are pouring). After filling the syringe
with slightly more than the desired amount, I carefully replace the plunger and
invert the syringe, remove the cap and expel the air. Then I discard the small
amount over what I need and then expel the measured portion into a disposable 50
ml polypropylene centrifuge tube. Brand does not matter as long as it has a
sealable top (I am currently using Falcon Bluemax 2098). I use the same syringe
for all of the first 3 components. I measure the DMP30 by placing the tip of a 2
or 3 ml syringe in the bottle and drawing up the desired amount into the
syringe.
After all the components have been place in the centrifuge tube, I mix by
inversion on a rotator for at least 15 minutes. I know many people say you
shouldn't mix by inversion because of the air bubbles but I find this is not a
problem if I let the mix set for 15 to 20 minutes after mixing.
My embedding protocol is as follows
50% ethanol for 15 minutes
70% ethanol for 15 minutes
95% ethanol for 15 minutes
100% ethanol for 15 minutes X2
100% propylene oxide for 15 minutes X2
1:1 Epon/araldite:propylene oxide for 1 hour
Pure Epon/Araldite for 1 hour
Place one piece of tissue on fresh plastic in a beem capsule and let it slowly
settle to the bottom of the capsule. After 3 to 4 hours place in oven at 80o C
overnight to polymerize.
If you need more info you can email me at msteglic-at-mdanderson.org.

Mannie Steglich
Tech Dir E M Lab
U T M D Anderson Cancer Center
Houston, TX




From daemon Wed Aug 20 17:02:33 2003



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Wed, 20 Aug 2003 18:12:20 -0400
Subject: Re: Ask-A-Microscopist : Whole Mounts

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Steve,
Karen, who was Dr. Keith Porter's Research Specialist, was in my
department several years back. They were studing fish scale cells
growing on gold grids that were coated but I do not recall with which
plastic. The grids were carbon coated indirectly (a shield was placed
between the rods and the grids). I do know that the grids were glow
discharged while still on the coverslip which was used to pick up the
coated grids. A thin strip of aluminum was used instead of the usual
copper wire for the glow. The grids were UV sterilized and I believe
they were put directly into the culture dishes with no
washing/rinsing. They did use 400 kV. to see more than just the edge
of the cells. I believe that this work was published before Dr.
Porter passed away.
Pat

Patricia Stranen Connelly
The University of Pennsylvania
Department of Biology
Philadelphia, PA 91904-6018
215-898-7145
psconnel-at-sas.upenn.edu

} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (ssamuelsson-at-eyetk.com) from
} http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html
} on Monday, August 18, 2003
} ---------------------------------------------------------------------------
} Email: ssamuelsson-at-eyetk.com
} Name: Steve Samuelsson
} Organization: Eyetech Pharmaceuticals
} Title-Subject: Whole Mounts
} Question: Regarding growing endothelial cells on nickel grids for
} whole mount TEM analysis: our luck is inconsistent and we now need
} to dig further for the reason. 200 and 300 mesh nickel grids are
} coated with ~0.5% formvar in ethylene dichloride (we also tried
} chloroform) and picked up on a 13mm cover slip. These are allowed
} to dry and age a day or longer. Prior to plating, the slips are UV
} sterilized 10-15', incubated in 1% gelatin ~45', washed 1x with PBS,
} culture medium added followed by cells. Our issue is incomplete (or
} downright lousy) growth of cells on the grids. Cells are robust and
} growing fine on the bottom of the 24 well plate so we suspect
} something with the grids. My first thought was that we needed
} carbon coating of the grids but have some trouble with this
} explanation. Second thought was that the solvent was still coming
} out of the films and poisoning the cells; thus the 'aging' issue
} with the cover slips. Any suggestions?
} ---------------------------------------------------------------------------



From daemon Wed Aug 20 18:25:42 2003



From: Rosemary White :      rosemary.white-at-csiro.au
Date: Thu, 21 Aug 2003 09:20:29 +1000
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This has happened at our institution also, but for short-sighted folk like
me who have to wear glasses on the microscope anyway, "they" made a real
effort to get reasonably comfortable and not too large fully enclosed
safety glasses with prescription lenses - must have cost a packet. They do
have to worry about insurance, etc, but here, at least, although it's an
annoyance, it's not too hard to follow the regulations.

}
} Now that the safety nazis have forced us to wear face shields, smocks and
} gloves for handling liquid nitrogen, they want us to wear safety glasses
} while doing wedge polishing, which entails frequent looking through a
} microscope to judge the progress. The concern is that some polish slurry
} 'could' splash into the eyes of a technician. Of course the safety glasses
} really get in the way when looking through a microscope, and our
} technicians are really not happy about this. I would like to hear as many
} opinions as possible on whether there is significant likelyhood of such a
} splash, and if polishing slurry, like Siton or Glanzox really poses a
} hazard to the eyes of a person using it. Thanks.
}
} John Mardinly
} Intel


Dr Rosemary White rosemary.white-at-csiro.au
Microscopy Centre fax 61- 2 6246 5000
CSIRO Plant Industry ph. 61- 2 6246 5475 or
GPO Box 1600 mob. 61- 0402 835 973
Canberra, ACT 2601, Australia




From daemon Wed Aug 20 18:46:43 2003



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 20 Aug 2003 13:40:44 -1000 (HST)
Subject: Re: Ask-A-Microscopist : Whole Mounts

Contents Retrieved from Microscopy Listserver Archives
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Hi, All-

Years ago I grew crustacean neurosecretory cells on Formvar-coated gold
grids, fixed, postfixed, dehydrated, and critical point dried them (we
had a grid holder for our Tousimis CPD) for 400 kV TEM. They were pretty
happy cells, and it worked well.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Aug 20 19:13:34 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 20 Aug 2003 17:08:54 -0700
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
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Being in the US university system for ten years now (wow, time is just
flying) I was and is amused how this system handles safety issue. Students
and most technical stuff have very little idea how to perform safe in the
laboratory environment. Safety issue is limited to some bureaucratic tasks
not related to the real safety (mostly keeping paperwork in a good shape
and accurately fill out chemical waste disposal tags). So, in such
situation, I think, it's superviser's personal responsibility to provide
safely environment to the workers and train them accordingly. I think, the
health issue is much, much more important than any personal or corporative
ambitious. If it's SAFER to use protective glasses, it's your
responsibility to ensure that your workers do use glasses. It's really
sad, but I agree at some degree with Chris Zurenko, that safety issue
sometime reflects not real carry about worker's health but attempt to avoid
legal responsibility (which, in my point of view, nothing to do with real
safety). Sergey

P.S. By the way: in my EM work, my glasses, I wear to correct my vision,
save my eyes in uncounted number of times. I, also, used to remove them
every time I am using light microscope or binoculars. I don't see big
problem removing them if necessary. But: yes, it decreases my
productivity. Ok, let say, I need about 5 sec. to remove them and put
back. Let say I do it 40 times a day: 5 secx40=200 sec/3.3 min per day.

At 12:36 AM 8/20/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Aug 20 20:20:01 2003



From: Christopher S. Zurenko :      czurenko-at-umich.edu
Date: Wed, 20 Aug 2003 21:13:14 -0400 (EDT)
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
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I'm not saying that I follow my own logic 100%. There are tasks that I
perform in the lab everyday that I probably "should" be wearing more
protective gear, but I don't, and I'm sure we all do. With regard to the
original post, I'm not sure that I would want to wear a full smock for
pouring liquid nitrogen either, but gloves and eye protection are a good
idea.

The point I'm trying to make is that in a day and age of fast food chains
getting sued because their fatty food makes people overweight and
cigarette companies handing out huge settlements because people now have
cancer, etc. (after doctors have been stating for YEARS that fatty food
and cigarettes are not good for you), companies can not be too careful.
That is the case here. The company implementing the eye guards does not
want someone to get abrasive compound in their eyes. They are attempting
to limit their risk and liability for someone being injured, very simple.
You would like to think that the company has acted on more of a
humanitarian agenda to provide for and protect its employees (and it may
be a small piece of the pie) but I think it's more to protect the company.

I don't see how legitimate research would be denied due to the
implementation of safety rules. There should be rules to limit risk, but
you can never 100% eliminate it. Legitimate research grinds on everyday
(following internal, local, state and federal safety mandates) and I don't
see that ending anytime soon.

Back to the original post - if using this eyewear is creating a major
problem for the type of research being conducted, talk with management and
the safety people to attempt to implement a mutually beneficial setup
where an eye splash could mostly be eliminated. Maybe some sort of Lexan
guards or shielding rather than eyewear. As far as the specific question
about the abrasive getting in the eye, I don't know but I don't think that
matters (though I can't imagine any sort of abrasive in the eye would ever
be a good or tolerable thing). It probably wouldn't matter if it's
abrasive, oil, sewage, tap water or sterile saline that possesses a risk
of getting slashed in the eye. Your safety people have identified a
safety risk and have moved to protect the employees and company. Whether
you or anyone else agrees with me or not, it is now the responsibility of
techs to wear the glasses and supervisors to make sure they are worn,
whether anyone is happy about it or not.

Cheers

--------------------------------------------------------------------
Christopher S. Zurenko
Research Assistant II
Kresge Hearing Research Institute, Otopathology
The University of Michigan Medical School
MSRB 3, Room 9303
1150 W. Medical Center Dr. Ann Arbor, MI 48109-0648




From daemon Thu Aug 21 04:29:40 2003



From: Francisco Freire :      sme-at-sgi.ulpgc.es
Date: Thu, 21 Aug 2003 10:19:41 +0000
Subject: Re: Humidity and Plastic Problems

Contents Retrieved from Microscopy Listserver Archives
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Garry:
Please check to storing conditions of your components, the DDSA and less the DMP
30 are very hidroscopyc. The last one can be replaced with BDMA with very good
results at ultramicrotomy time.
Have a good luck!

Francisco Freire FRMS
Head
Electron Microscopy Service
University of Las Palmas de Gran Canaria
Canary Islands
Spain





From daemon Thu Aug 21 04:49:37 2003



From: gillian.2.brown-at-gsk.com
Date: Thu, 21 Aug 2003 10:45:39 +0100
Subject: Re: Safety Glasses whilst polishing/microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my labs it is mandatory to wear safety glasses.
Risk assessments are carried out on all microscope usage and, if deemed
safe, can be given an exemption sticker (signed off by management) so the
user can remove specs. That RA would cover what activities are occurring
adjacent and whether the operative has wet hands/gloves as any
contaminants could be transferred to hair/skin and indeed eyes during
removal of the glasses.

Where the 'environment' remains unsafe we are now moving to replacing the
eyepieces with those suitable for spectacle wearers (safety or not). Non
spectacle users in Histopath labs here are also having them fitted for
doing their 'safe' microscopy as they prefer the ergonomics of them.

So change the microscopy set up and every-one should be safe and happy.

Gillian Brown
Histology Section, Asthma Biology
RI CEDD, GSK.




From daemon Thu Aug 21 06:24:05 2003



From: atcsem-at-earthlink.net
Date: Thu, 21 Aug 2003 07:24:11 -0400
Subject: Possible virus Re: Your details

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After a massive (or at least sobig) virus attack I'm back online.

That's a very interesting point Sergej makes.

We work with thin (1 layer) protein crystals and large individual protein
molecules (chaperones etc.).

For crystals the flatness of the carbon surface is obviously important
but for single proteins I've always thought it shouldn't make any
difference.
Initially we use negative stain to look at the quality of a specimen.
To get higher resolution data we use cryo-specimens on holey carbon so
that is a different story.
Back to the negative stain: I've been wondering about two possible effects
when staining:
1. When you stain a specimen on the rough side the stain might fill all the
valleys
of the carbon surface leading to large background noise and making it
difficult
to see the particles (some grids seem to have a lot of background).
2. When you stain a specimen on the smooth side (which is facing the grid)
the stain
might get trapped between the grid bars leading to an unnecessarily thick
layer of
stain which again increases the noise.

Are either of the two real problems?


Philip Koeck
Svdertvrns Hvgskola and
Karolinska Institutet
Dept. of Bioscience at Novum
S-14157 Huddinge
Sweden
phone: +46-8-6089186
fax: +46-8-6089290
http://www.biosci.ki.se/em
_______________________________________


----- Original Message -----
} From: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, August 18, 2003 10:06 PM


The attachments with .pif extensions

This is the virus you may have.
http://securityresponse.symantec.com/avcenter/venc/data/w32.sobig.f-at-mm.html

Note that the virus is spoofing e-mail addresses. so your system could be
clean.

Pavel Lozovyy
ATC SEM Lab
Phone: 216-692-6637
E-mail: atclabs-at-sbcglobal.net
web: www.atclabs.com



From daemon Thu Aug 21 09:45:18 2003



From: paul r hazelton, PhD :      paul_hazelton-at-umanitoba.ca
Date: Thu, 21 Aug 2003 09:38:25 -0500
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
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john

first, i am not really sure what the position of chris jeffree is, i
found his response a bit confusing. sergey's comments considering time
are to the point, and rosemary describes the situation i have
experienced quite accurately. from my view, after thirty years i still
have my eyes. last time i checked, they were a bit important for my
work.

in a previous persona (before i grew up and went to graduate school) i
had the privilege of negotiating collective agreements on behalf of an
independent union which represents university non-academic staff. at
the university of manitoba this means about 2000 administrative,
clerical, computer, library, security and technical employees. the
technical staff were, and still are about 40% of the population. for
the record, i was chief negotiator, not member of the team, and in one
of those cases we were on strike for 7 weeks. hopefully this sets it
clear with chris zurenko that i was not a management stooge. the issue
of safety supplies and equipment was always important, even in the dark
ages of 1975. in fact, historically most improvements in working
conditions and staff safety are the result of labour organizations
fighting for them. five day work weeks, vacations, and child labour
laws were not the result of an insurance company telling managers they
had to make the changes. they were the result of the workers fighting
for better and safer working conditions. in our case, we identified
problems long before any insurer or government agency ever did. what
is most important is that the university of manitoba, at least, never
argued the importance of the issues. usually, our only problem was
figuring out how we could ensure payment for the needed materials in
individual grant funded positions.

having worked in the university research system for over 30 years i have
seen most different approaches. to make it clear, i do not think
universities are any more concerned with safety than any employer. have
the 'safety nazis' made it more difficult, yes. but at least the safety
issues are being dealt with in many areas, not by a few benevolent
employers. no insurance payment will compensate for the use of a hand,
hearing, life, or in most of our cases, an eye. if you have a problem
with implimentation do what rosemary did, discuss the methods,
rationally. do it as if you were defending your thesis again. sell the
problem and get a cure. but let's listen to sergey and not complain
about the few seconds needed to take off glasses or shields.

finally, to make it clear, i learned my attitudes from my manufacturer
father, who taught me that the most important resource he had was his
workers. i am not a left wing pinko, i am very middle of the road. and
perhaps a little to the right of centre on economic issues

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926





From daemon Thu Aug 21 10:17:02 2003



From: Fred.Hayes-at-colaik.com
Date: Thu, 21 Aug 2003 11:19:40 -0400
Subject: bound vs free plasticizer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone recommend a microscopy method/technique to visualize and/or
possibly quantify the amount of free plasticizer vs the amount of bound
plasticizer in cast PVC skins? We suspect a ratio difference of free vs
bound plasticizer when comparing PVC raw materials vs cast PVC skins which
are exhibiting adhesive failure between the PVC skin and foam. We think
there may be a higher amount than normal, of free plasticizer migrating
to the PVC skin surface.

Fred A. Hayes
Polymer Microscopy
Collins & Aikman
IntelliMold Div
4651 Platt Lane
Ann Arbor, MI 48108
734-477-7029 office
734-477-9214 fax


From daemon Thu Aug 21 23:05:09 2003



From: Materials Research Laboratories, Inc. :      mrllab-at-raex.com
Date: Thu, 21 Aug 2003 23:53:46 -0400
Subject: Re: Possible virus Re: Your details

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Only for windows. Mac users never have the problem. :) I received about
30..opened some..no problem. :)

on 8/21/03 7:24 AM, "atcsem-at-earthlink.net"-at-sparc5.microscopy.com at
"atcsem-at-earthlink.net"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The attachments with .pif extensions
}
} This is the virus you may have.
} http://securityresponse.symantec.com/avcenter/venc/data/w32.sobig.f-at-mm.html
}
} Note that the virus is spoofing e-mail addresses. so your system could be
} clean.
}
} Pavel Lozovyy
} ATC SEM Lab
} Phone: 216-692-6637
} E-mail: atclabs-at-sbcglobal.net
} web: www.atclabs.com
}
}
}



From daemon Fri Aug 22 04:31:59 2003



From: Philip Koeck :      Philip.Koeck-at-biosci.ki.se
Date: Fri, 22 Aug 2003 11:25:31 +0200
Subject: Re: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul and others

I thought I might as well add a point or two.

In Europe (I am not sure of the USA) there is a general system of controlling exposure to hazards which involves in order of preference:
1.Prevent exposure (eg remove the hazardous process or substitute hazardous material with safer one).
2.Control process by design/engineering (eg shielding, fume hood).
3.Use procedural controls (eg less people involved, good hygiene, reduce quantities).
4.and only finally use PPE (personal protective equipment) such as safety glasses.

If the first three can be applied successfully then there should be no need for PPE such as safety glasses. An obvious point may be to separate the preparation procedure from the microscopy because if there is a risk of splashing the eyes then surely there is a risk of splashing the optics of microscopes.

It should also be borne in mind that if an accident (at least in the UK) results in an injury and the employer didn't take reasonable precautions and enforce them then that employer could be deemed to be negligent. Health and safety issues are often covered by criminal law so the employer or their representative could go to prison (doesn't often happen - but can) and any damages would not normally be insurable so the employer would have to pay and not the insurer. It gives a different slant on the "safety nazis" .

I am still a union health and safety officer and so my sympathies are with the staff rather than employers but I do recognize how difficult a job employers can sometimes have.

I'm sorry for extending an already lengthy debate but the principles are extremely important and I have tried to be brief.

Malcolm Haswell
e.m. unit
University of Sunderland
UK

----- Original Message -----
} From: "paul r hazelton, PhD" {paul_hazelton-at-umanitoba.ca}


Sergey!
You say that you can't use the bar side of the grids.
Why is that so clear?
A thicker layer of stain might not be a disadvantage as long as
it is even.

(I'm assuming specimens that are much smaller than the mesh)

Philip


} Phillip - you right: on the rough surface negative stain fills the
} imperfections of the surface and therefore increase background noise. It
} also makes stain's layer thicker (holding more stain then necessary). All
} these things decrease the resolution of your images. You could not use,
of
} coarse, the opposite side of the grid with bars. So, you need to put flat
} side of the carbon up: mount your grids ON the floating carbon. If you
are
} trying to do high resolution EM on individual proteins, you definitely
need
} to pay attention to the quality of the carbon: this is a whole point to
} use "mica-side" of the carbon, because mica surface is atomically perfect
} (it's a single crystal), so the carbon surface will be nearly perfect as
} well. The quality of mica is important too. Have a good day, Sergey.
}
}
} }
} }
} } After a massive (or at least sobig) virus attack I'm back online.
} }
} } That's a very interesting point Sergej makes.
} }
} } We work with thin (1 layer) protein crystals and large individual protein
} } molecules (chaperones etc.).
} }
} } For crystals the flatness of the carbon surface is obviously important
} } but for single proteins I've always thought it shouldn't make any
} } difference.
} } Initially we use negative stain to look at the quality of a specimen.
} } To get higher resolution data we use cryo-specimens on holey carbon so
} } that is a different story.
} } Back to the negative stain: I've been wondering about two possible
effects
} } when staining:
} } 1. When you stain a specimen on the rough side the stain might fill all
the
} } valleys
} } of the carbon surface leading to large background noise and making it
} } difficult
} } to see the particles (some grids seem to have a lot of background).
} } 2. When you stain a specimen on the smooth side (which is facing the
grid)
} } the stain
} } might get trapped between the grid bars leading to an unnecessarily thick
} } layer of
} } stain which again increases the noise.
} }
} } Are either of the two real problems?
} }
} }
} } }
} } } Phillip
} } } Your way to mount carbon on the grids has one very serious
disadvantage:
} }
} } } when you mount carbon this way, you will use "air-side" of carbon as a
} } } working surface. This side is usually more grainy and dirty. The
whole
} } } idea of using carbon from mica is to use "mica-side" of carbon as a
} } working
} } } surface. The idea here is that the carbon face toward mica is more
} } uniform
} } } and perfectly clean (it was not exposured to the air). In order to use
} } } "good" side of carbon, you have to put grids on floated carbon and
then
} } } pick the grids with Parafilm for instance. Similarly, you need to do
the
} } } same for plastic film created on water - working side is "water-side",
not
} } } air. Only one exception I do know is Formvar film created on the
} } } glass. Glass is not such clean and perfect as mica or water surface,
} } } therefore, you need to use "air-side". Then your technique will work
} } } perfectly. I also use your technique to mount holey film on the
grids.
} } } Best wishes, Sergey.
} } }
} } }
} } } }
} } } }
} } } } You can easily use the method described by Mike to make
} } } } about 50 pure carbon grids at a time (much cheaper than
} } } } buying if you already have the equipment).
} } } }
} } } } You place about 50 grids on a square piece of filter paper
} } } } lying on a little "table" made of wire mesh under the water surface.
} } } } To get the grids under water make them wet first and push them
} } } } through the surface vertically.
} } } } Then you lower the water surface by removing water from underneath
} } } } (for example with a syringe or a plastic tube with a valve) to place
} } } } the carbon film (floated off the mica) on top of the grids.
} } } } (Of course the carbon film has to be in one piece in this case.)
} } } } Push the carbon into position with something blunt (a paddle ? :)
} } } }
} } } } Philip Koeck


} } } } } } Steve,
} } } } } } When I want just carbon as a substrate I will
} } } } } } coat freshly cleaved mica with carbon in a metal
} } } } } } evaporator. This allows me to dictate the carbon
} } } } } } thickness and I have no trouble floating off grid
} } } } } } size squares(prescored)in a drop of water. Picking up the
} } } } } } carbon with glow discharged grids (flat side)
} } } } } } is a snap. Make sure everything is clean.
} } } } } } This should give you thin clean substrates.
} } } } } } Mike D.
} } }
} } }
}



From daemon Fri Aug 22 07:21:12 2003



From: Tomic, Peter (Peter) :      ptomic-at-agere.com
Date: Fri, 22 Aug 2003 08:11:00 -0400
Subject: Re: Safety Glasses whilst polishing/microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gillian;

You mentioned a good point with regard to chemistry and microscopy, wet gloves. Many people do etching, polishing, and wet chemistry with iterations between a hood/sink and a microscope. Some also feel that they are saving time/money by not removing their gloves whilst adjusting the focus, stage position and worst of all the eyepieces. I can say from experience how this caused me great pain and an emergency hospital visit because someone had their gloved hands in nitric acid and decided to adjust the inter-ocular distance on a microscope that I was alternately using.

My rule was that no gloved hand may touch a microscope whether it's been in a hood, sink or whatever. This way there can be no mistake unless some fool likes to work with bare skin, and there is no protecting oneself from the super-idiot. The only remedy for them is banishment to a desk job where the limit of injury is paper cuts or burns from hot coffee.

Peter Tomic
Agere Systems
Allentown, PA

-----Original Message-----
} From: "gillian.2.brown-at-gsk.com"-at-sparc5.microscopy.com
[mailto:"gillian.2.brown-at-gsk.com"-at-sparc5.microscopy.com]
Sent: Thursday, August 21, 2003 5:46 AM
To: microscopy-at-sparc5.microscopy.com


In my labs it is mandatory to wear safety glasses.
Risk assessments are carried out on all microscope usage and, if deemed
safe, can be given an exemption sticker (signed off by management) so the
user can remove specs. That RA would cover what activities are occurring
adjacent and whether the operative has wet hands/gloves as any
contaminants could be transferred to hair/skin and indeed eyes during
removal of the glasses.

Where the 'environment' remains unsafe we are now moving to replacing the
eyepieces with those suitable for spectacle wearers (safety or not). Non
spectacle users in Histopath labs here are also having them fitted for
doing their 'safe' microscopy as they prefer the ergonomics of them.

So change the microscopy set up and every-one should be safe and happy.

Gillian Brown
Histology Section, Asthma Biology
RI CEDD, GSK.




From daemon Fri Aug 22 08:49:07 2003



From: paul r hazelton, PhD :      paul_hazelton-at-umanitoba.ca
Date: Fri, 22 Aug 2003 08:41:56 -0500
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
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malcolm

your points are very good. i don't know that i have seen them written
down here, but they fit exactly the process we use here. there is heavy
emphasis on point three. procedure controls. if a person is only going
to do something only 1x or 2x, we do it for them. this does not matter
if it is dealing with pathogens, radiation, osmium, or other chemical or
biologicals.

for what it is worth, i have been trying to eliminate all use of
radioactives from my work - not because of the necessary controls and
protection, but because of the paper work, that unmentioned
administrative point, #5.

thanks for the points, i will forward them to my former union.

paul



From daemon Fri Aug 22 10:47:07 2003



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 22 Aug 2003 16:40:40 +0100 (BST)
Subject: Re: Ask-A-Microscopist: optical polarization

Contents Retrieved from Microscopy Listserver Archives
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Hafeez,

I see you've already had a detailed explanation (which I've lost -
accidentally deleted because of something odd in my software - never mind,
I can pick it up again once it's in the archive), however, I would like to
share with you some experience of observing polymer spherulites under the
polarizing microscope.

Normally, one observes spherulites between crossed polars, and if the
birefringence is strong, you get a four lobed pattern with colours in the
sequence you also observe with the quartz wedge. However, if you rotate
the analyser to be parallel with the polarizer, you see different colours,
in a two lobe pattern. These colours are the complementary ones to those
observed between crossed polars. Going from no birefringence to just over
the 1st order, one has:

Crossed - Parallel

Black - White (zero order)
Grey - not so bright
Yellow - Dark Blue
Purple - Green (1st order)
Blue - Reddish Orange

I have often looked at polymers (including blends) under the polarizing
optical microscope. I do not find that rotating the analyser gives extra
information, it simply changes the colour of the presentation. However,
rotating the specimen between crossed polars, and watching the colours
brighten and darken (though they remain the same colour) does give
information about the orientation of the molecules.

Hope this is of use.

+-----------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+----------------------------------------+
Phone:+44 (0) 118 9318572
Fax: +44 (0) 118 9750203
University internal extension 7867
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+----------------------------------------+



From daemon Fri Aug 22 13:13:37 2003



From: MIngram-at-rodel.com (by way of MicroscopyListserver)
Date: Fri, 22 Aug 2003 13:06:44 -0500
Subject: Lab SEM for 200 mm Wafers

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All,

My company is in the semiconductor industry. We current have an AMRAY 2030 C for performing defect review on 8-inch wafers with the ability to read defect files from wafer inspection tools. Our AMRAY is on its last legs.

Are there lab SEM available that can inspect 8-inch wafers edge to edge with the ability to read defect files?

The semiconductor SEM's are expensive and restrictive for r&d work. They are design for production purposes and many SEM controls are removed or automated that limit the tools usefulness.

Thank

Mike Ingram

Rodel, Inc.


From daemon Sat Aug 23 11:00:20 2003



From: yezer-at-barak-online.net (by way of MicroscopyListserver)
Date: Sat, 23 Aug 2003 10:43:36 -0500
Subject: MListserver: fast glue which is compatible with UHV

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (yezer-at-barak-online.net) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, August 23, 2003 at 10:16:49
---------------------------------------------------------------------------

Email: yezer-at-barak-online.net
Name: Ezer Yosi

Title-Subject: MListserver:

Question: I am looking for a fast glue which is compatible with UHV (for TEM sample).
Can you kindly recomend on one of loctite system for this application.

Sincerely

Ezer Yossef

---------------------------------------------------------------------------


From daemon Sun Aug 24 09:51:15 2003



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 24 Aug 2003 10:31:00 -0500
Subject: UHV compatible "glue"

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ezer Yossef wrote:
=====================================================
Question: I am looking for a fast glue which is compatible with UHV (for TEM
sample). Can you kindly recomend on one of loctite system for this
application.
---------------------------------------------------------------------------
The M-Bond 610 system seems to be the most widely used glue for this
application, and it can be purchased from SPI Supplies (or some of the other
major supply houses for EM consumables) and is described on URL
http://www.2spi.com/catalog/spec_prep/glue.shtml

I do hear reports of some who claim to get comparable results with some of
the "5 minute" epoxy systems, such as is shown on URL
http://www.2spi.com/catalog/spec_prep/5minglue.shtml

The latter is obviously much cheaper but most people seem to prefer M-Bond
610 (or possibly other alternatives that might be comparable to M-Bond 610).

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================






From daemon Sun Aug 24 17:34:39 2003



From: Pacific GridTech :      wendy-at-grid-tech.com
Date: Sun, 24 Aug 2003 15:27:25 -0700 (PDT)
Subject: Re: Carbon Support Films for TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi, Philip,

One more thing add under your message. The Mo grid
with very smooth hole will provide the most flat
carbon film without cryo-wrink. For more details,
please cheak Pacific Grid-Tech website.

http://www.grid-tech.com/

regards

Wendy

}
-----------------------------------------------------------------------.
}
}
} After a massive (or at least sobig) virus attack I'm
} back online.
}
} That's a very interesting point Sergej makes.
}
} We work with thin (1 layer) protein crystals and
} large individual protein
} molecules (chaperones etc.).
}
} For crystals the flatness of the carbon surface is
} obviously important
} but for single proteins I've always thought it
} shouldn't make any
} difference.
} Initially we use negative stain to look at the
} quality of a specimen.
} To get higher resolution data we use cryo-specimens
} on holey carbon so
} that is a different story.
} Back to the negative stain: I've been wondering
} about two possible effects
} when staining:
} 1. When you stain a specimen on the rough side the
} stain might fill all the
} valleys
} of the carbon surface leading to large background
} noise and making it
} difficult
} to see the particles (some grids seem to have a lot
} of background).
} 2. When you stain a specimen on the smooth side
} (which is facing the grid)
} the stain
} might get trapped between the grid bars leading to
} an unnecessarily thick
} layer of
} stain which again increases the noise.
}
} Are either of the two real problems?
}
}
} Philip Koeck
} Svdertvrns Hvgskola and
} Karolinska Institutet
} Dept. of Bioscience at Novum
} S-14157 Huddinge
} Sweden
} phone: +46-8-6089186
} fax: +46-8-6089290
} http://www.biosci.ki.se/em
} _______________________________________
}
}
} ----- Original Message -----
} } From: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Monday, August 18, 2003 10:06 PM
} Subject: Re: Carbon Support Films for TEM
}
}
} }
}
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
-----------------------------------------------------------------------.
} }
} }
} } Phillip
} } Your way to mount carbon on the grids has one very
} serious disadvantage:
}
} } when you mount carbon this way, you will use
} "air-side" of carbon as a
} } working surface. This side is usually more grainy
} and dirty. The whole
} } idea of using carbon from mica is to use
} "mica-side" of carbon as a
} working
} } surface. The idea here is that the carbon face
} toward mica is more
} uniform
} } and perfectly clean (it was not exposured to the
} air). In order to use
} } "good" side of carbon, you have to put grids on
} floated carbon and then
} } pick the grids with Parafilm for instance.
} Similarly, you need to do the
} } same for plastic film created on water - working
} side is "water-side", not
} } air. Only one exception I do know is Formvar film
} created on the
} } glass. Glass is not such clean and perfect as
} mica or water surface,
} } therefore, you need to use "air-side". Then your
} technique will work
} } perfectly. I also use your technique to mount
} holey film on the grids.
} } Best wishes, Sergey.
} }
} }
} } At 04:07 PM 8/18/03 +0200, you wrote:
} }
}
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} }
} } http://www.msa.microscopy.com/MicroscopyListserver
} } } On-Line Help
}
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} -----------------------------------------------------------------------.
} } }
} } }
} } } You can easily use the method described by Mike
} to make
} } } about 50 pure carbon grids at a time (much
} cheaper than
} } } buying if you already have the equipment).
} } }
} } } You place about 50 grids on a square piece of
} filter paper
} } } lying on a little "table" made of wire mesh under
} the water surface.
} } } To get the grids under water make them wet first
} and push them
} } } through the surface vertically.
} } } Then you lower the water surface by removing
} water from underneath
} } } (for example with a syringe or a plastic tube
} with a valve) to place
} } } the carbon film (floated off the mica) on top of
} the grids.
} } } (Of course the carbon film has to be in one piece
} in this case.)
} } } Push the carbon into position with something
} blunt (a paddle ? :)
} } }
} } } Philip Koeck
} } } Svdertvrns Hvgskola and
} } } Karolinska Institutet
} } } Dept. of Bioscience at Novum
} } } S-14157 Huddinge
} } } Sweden
} } } phone: +46-8-6089186
} } } fax: +46-8-6089290
} } } http://www.biosci.ki.se/em
} } } _______________________________________
} } }
} } } } } Steve,
} } } } } When I want just carbon as a substrate I
} will
} } } } } coat freshly cleaved mica with carbon in a
} metal
} } } } } evaporator. This allows me to dictate the
} carbon
} } } } } thickness and I have no trouble floating off
} grid
} } } } } size squares(prescored)in a drop of water.
} Picking up the
} } } } } carbon with glow discharged grids (flat side)
} } } } } is a snap. Make sure everything is clean.
} } } } } This should give you thin clean substrates.
} } } } } Mike D.
} }
} }
}
}


=====
Pacific GridTech
A high quality EM grid provider
3505 Caminito Carmel Landing
San Diego, CA 92130, USA
Tel: (858) 336 8938; Fax: (858) 259 5511
Email: info-at-grid-tech.com
Web: http://www.Grid-Tech.com/


From daemon Sun Aug 24 18:18:34 2003



From: Walck, Scott D. :      walck-at-ppg.com
Date: Sun, 24 Aug 2003 19:13:20 -0400
Subject: RE: UHV compatible "glue"

Contents Retrieved from Microscopy Listserver Archives
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First off, let me comment about UHV and TEM. UHV is generally considered to be in the vacuum range where the pressure is less than 1E-9 Torr. Although the microscope manufacturers would like you to believe that your sample is setting in an UHV environment, chances are it isn't. The dedicated STEM systems may come close. The reason for this is that the pressure is typically measured using the ion pump or a gauge located close to the pump flange. Your sample sits at the end of a conductance limited tube that connects to your pump. In vacuum systems, the throughput (quantity of gas per unit time), Q (Torr l/sec) = S * P, where S is the pump speed (l/sec) and P is pressure (Torr). S * P at the pump is equal to S(eff)*P(sample). S(eff) is the effective pump speed at your sample and is related to both the conductance and pump speed. However, if your diameter of the tubing is small and the length is long or has bends in it, the effective pump speed will be conductance limited and is
approximately the same as the conductance. The net effect is that the effective pump speed at your sample is always less than the pump speed and as a result, the true pressure at your sample will be higher than the reported pressure. With all that said, if you cool an anti-contamination device in the neighborhood of your sample, that acts as a cryopump and will lower the pressure at your sample.

For high vacuum systems, epoxy based materials will work fine. However, you should make sure that the mix is right so that everything cross links well and you don't have any of either the hardener or the resin "left over". You should also minimize the amount that you use. Epoxies that have worked well in TEM sample prep are M-bond 610 (as Chuck Garber said), Gatan's G-1 which is equivalent to Epoxy Technologies' Epo-Tek 353ND. A silver filled conductive epoxy that works well is Epoxy Technologies' H-22. Instead of the 5 or 10 min epoxies, I would recommend the 90 min working time, super strength types that require an overnight cure.

If your interested in true, UHV compatible "glues", you are in trouble. There are two sealers that people use for this purpose in UHV systems. One is a product called VacSeal(R) and comes in a brush-on bottle form or a spray application. This stuff works very well when it is room temperature dried, but works even better with a 200C cure. I doubt that it would work well for TEM sample prep. Another material that is used is a two part putty-like material that used to be available from Perkin Elmer. Sorry, I can't remember the name. I would check with vacuum component manufacturers for the name. You might also check with Kurt Lesker. People have also used the water based "Aquadawg" or "Aquadog" to tack things down in UHV systems, but it has to be heated to drive off the water.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



From daemon Mon Aug 25 11:45:23 2003



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Mon, 25 Aug 2003 12:25:20 -0400
Subject: Looking for User input on EBSD systems

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We are in the market for an EBSD system. (I believe all the relevant
vendors already know this.) This is a very new area for us and we have
basically no experience in this area (to be very blunt). We are dealing with
technical specs, vendor information, and demos. And as good and as hands-
on as demos maybe they can not give you the true picture of working with a
system for months or years or multiple users. What we are looking for is
information from USERS of EBSD systems (Researchers, facility managers,
etc.) - we are looking for comments, opinions, pro & cons, experiences of any
EBSD systems.

Please send your comments directly to me so you maybe as candid as
possible.

Thank you.




Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Mon Aug 25 13:16:16 2003



From: Barbara Foster :      bfoster-at-mme1.com
Date: Mon, 25 Aug 2003 14:09:45 -0700
Subject: Re: bound vs free plasticizer

Contents Retrieved from Microscopy Listserver Archives
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Hi, Fred,

I'd suggest you investigate both Raman confocal and FT-IR microscopy.

The FT-IR is probably easier to do, if you can get cross sections. Otherwise, Raman confocal can go down layer by layer. I don't think that there is any limitation for either system in terms of the chemical fingerprint they can provide to you. The bound v. unbound may have a better signature in Raman, however.

A number of companies, including BioRad and Nicolet, provide FT-IR microscopes. SensIR (Danbury, CT) provides the IlluminatIR - a unique microscopy accessory which turns a research microscope into an FT-IR microprobe without disabling the microscope.

Renishaw and Jobin-Yvon (JY) both provide Raman confocal.

All of the companies have apps labs which can run a sample or two for you. I'd suggest you visit
microscopy.info for contact information. I'm going to be on vacation most of this week, but if you get stuck, drop me an email and I'll send you more specific contact information.

Good hunting!
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suite 102
Springfield, MA 01118
PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com

^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Optimizing Light Microscopy is still available through MME. For information on ordering single or small quantities, visit our website. For discounts on classroom-sized, call us today.
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
At 11:19 AM 8/21/03 -0400, Fred.Hayes-at-colaik.com"-at-sparc5.microscopy.com wrote:
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From daemon Mon Aug 25 14:52:47 2003



From: bonnie.davis-at-kennametal.com
Date: Mon, 25 Aug 2003 15:43:08 -0400
Subject: EM Position Opening

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(Please do not respond to this e-mail. Send all responses to the contact
listed below)



A position is available in the Materials Science and Engineering Department
at Carnegie Mellon University (http://neon.mems.cmu.edu/newfront.shtml) for
a full time staff member with experience in transmission electron
microscopy. The position will focus on analysis of ceramic thin films
having applications in the areas of electronics, magnetics, hard coatings,
and catalysis. A significant portion will focus on nano-layered thin films
for use as hard coatings. Tasks will include sample preparation (of
plan-view and cross-sectional samples), electron microscopy (including
high-resolution), and analysis of structural details (such as crystal,
interfacial, grain, and superlattice structures). The position could be
filled either at the research scientist or research associate
(postdoctoral) level. Salary will be commensurate with experience. Carnegie
Mellon University is an equal opportunity employer.

For Details and Inquiries Please Contact
Paul Salvador
Assistant Professor
Department of Materials Science and Engineering
Wean Hall 3301
Carnegie Mellon University
Pittsburgh, PA 15206
TEL: 412-268-2702, FAX: 412-268-7596
paulsalvador-at-cmu.edu




From daemon Tue Aug 26 05:57:41 2003



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Tue, 26 Aug 2003 11:44:34 +0100
Subject: TEM - PIPS

Contents Retrieved from Microscopy Listserver Archives
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To all those people out there who use the Gatan PIPS -

I thought this was common knowledge, but maybe not. Instead of polishing
the sputtered deposits off the viewing window after every hour or so of
milling time, just use a grade 0, 19 mm diameter glass cover slip. It sits
nicely in the recess under the window - the only precaution you have to take
is to make sure it doesn't slide to partially cover the O-ring. They are
cheap and can be considered disposable, but if you are really tight and have
a big jar of KOH in the cupboard (like me) you can clean the deposits off by
boiling in caustic solution for a while and re-use them.

I just hate to think of all those cumulative hours spent by users around the
world polishing glass when there is a simple alternative!

Cheers,

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356775
http://www.bookham.com





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From daemon Tue Aug 26 09:19:48 2003



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 26 Aug 2003 15:11:46 +0100 (GMT Daylight Time)
Subject: Poly-L-lysine

Contents Retrieved from Microscopy Listserver Archives
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I would like to try poly-L-lysine for sticking cells to
coverslips etc for dehydration and SEM. My S**** catalogue
offers about 15 variants. Can anyone advise me on which
type to use?

Dave

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Aug 26 11:29:23 2003



From: Louro, Pedro :      pedro.louro-at-spcorp.com
Date: Tue, 26 Aug 2003 12:19:07 -0400
Subject: eye fixatives?

Contents Retrieved from Microscopy Listserver Archives
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I'm looking for any feedback on fixation of eyes (small and large animal).
More specifically: a fixative that would work well for Histology and EM.
Any info. on:
* Glutaraldehyde
* Karnovsky's Fixative
* McDowell - Trump's Fixative
or any other fixative would be greatly appreciated.

Thanks in advance,

Pedro




*********************************************************************
This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete.



From daemon Tue Aug 26 12:22:01 2003



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 26 Aug 2003 10:12:08 -0700
Subject: RE: UHV compatible "glue"

Contents Retrieved from Microscopy Listserver Archives
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The 2-part epoxy is called Torr-seal, and is is very good.
--John Mardinly

material that is used is a two part putty-like material that used to be available from Perkin Elmer. Sorry, I can't remember the name. I would check with vacuum component manufacturers for the name. You might also check with Kurt Lesker. -Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)




From daemon Tue Aug 26 17:27:35 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 26 Aug 2003 15:18:29 -0700
Subject: Re: Poly-L-lysine

Contents Retrieved from Microscopy Listserver Archives
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David
There is some trick with Poly-Lysine solution. I would suggest to use some
from EM major suppliers. Personally I am using 1% solution from TedPella.
I don't think there is a difference between different brands. In England
you may contact Agar, very good EM supplier. Good luck, Sergey.

At 07:11 AM 8/26/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Aug 27 00:14:16 2003



From: AHMED, TOSEEF :      TOSEEF-at-sabic.com
Date: Wed, 27 Aug 2003 08:00:37 +0300
Subject: TEM Analysis of HIPS

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I am looking for a lab that can perform TEM analysis of high impact polystyrene. I intend to obtain an extended support for next few months in order to analyze 30-35 samples. The analysis involves specimen preparation (cryomicrotomy, staining), imaging at 2-3 reasonable magnifications and calculation of rubber volume fraction/size of each sample. I will appreciate the help of the list members in this regard. The interested labs on the list server may want to contact me of line for further discussions regarding the project.

Best regards.


Toseef Ahmed, Ph.D.
Electron Microscopy and Thermal Labs, Analytical Section
SABIC R&T, P.O. Box 42503, Riyadh 11551, Saudi Arabia
Tel: (966-1) 265-3333 Ext.6003, 6104
Fax: (966-1) 265-1101
E-mail: toseef-at-sabic.com,



From daemon Wed Aug 27 03:28:30 2003



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 27 Aug 2003 09:20:06 +0100 (GMT Daylight Time)
Subject: Poly-L-lysine: clarification

Contents Retrieved from Microscopy Listserver Archives
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My original post was ambiguous. I am looking for ways to
stick fixed cells to coverslips to avoid repeated
centrifugation during dehydration for SEM.

Dave


On Tue, 26 Aug 2003 15:11:46 +0100 (GMT Daylight Time)
"Patton, David" {David.Patton-at-uwe.ac.uk} wrote:

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}
}
} I would like to try poly-L-lysine for sticking cells to
} coverslips etc for dehydration and SEM. My S**** catalogue
} offers about 15 variants. Can anyone advise me on which
} type to use?
}
} Dave
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}
}
}
} This incoming email to UWE has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Aug 27 07:37:43 2003



From: Owen P. Mills :      opmills-at-mtu.edu
Date: Wed, 27 Aug 2003 08:31:14 -0400
Subject: University FIB rates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Torr-Seal is available from many of the supply houses including Ladd.
See
http://www.laddresearch.com/General_Catalog/Chapter_11/Miscellaneous_Vacuum_
Accessori/miscellaneous_vacuum_accessori.html
for our selection. MSDS is also available on line for those seeking more
information.

Debra Sicard
Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com
----- Original Message -----
} From: "Mardinly, John" {john.mardinly-at-intel.com}
To: "Walck, Scott D." {walck-at-ppg.com} ; "Microscopy (E-mail)"
{microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, August 26, 2003 1:12 PM


Hi all,

We are a University microscopy recharge center and will be installing a
single beam FIB within the next 6 months. I've got to establish a
reasonable initial hourly use rate without the benefit of experience with a
FIB costs and use patterns. Will you university FIB users out there share
some of your experience, wisdom and rates? Off line will be best I think.

Owen

Owen P. Mills
Electron Optics Engineer
Materials Science & Engineering
Michigan Technological University
Rm 512 M&M Bldg.
Houghton, MI 49931
PH 906-369-1875
FAX 906-487-2934
mailto:opmills-at-mtu.edu
http://www.mm.mtu.edu/~opmills




From daemon Wed Aug 27 08:02:46 2003



From: maloneyb-at-fiu.edu (by way of Ask-A-Microscopist)
Date: Wed, 27 Aug 2003 07:57:36 -0500
Subject: Ask-A-Microscopist: Philips 200 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (maloneyb-at-fiu.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, August 25, 2003 at 14:36:09
---------------------------------------------------------------------------

Email: maloneyb-at-fiu.edu
Name: Barb

Organization: FIU

Title-Subject: Phillips 200 TEM

Question: Does anyone have the specs on this particular model? Need ASAP
Thanks
Barb

---------------------------------------------------------------------------


From daemon Wed Aug 27 08:02:50 2003



From: info-at-klughammer.de (by way of Ask-A-Microscopist)
Date: Wed, 27 Aug 2003 07:58:16 -0500
Subject: Ask-A-Microscopist: EPI fluorescence microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (info-at-klughammer.de) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 27, 2003 at 03:42:53
---------------------------------------------------------------------------

Email: info-at-klughammer.de
Name: Anneliese Schmaus

Organization: klughammer gmbh

Title-Subject: MListserver:

Question: Hi,

does anybody know a EPI fluorescence microscope manufacturer which offers quite good quality at an affordable price. They will be used for university beginner groups.

Thanks to all

Anneliese Schmaus

---------------------------------------------------------------------------


From daemon Wed Aug 27 11:27:50 2003



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 27 Aug 2003 11:22:20 -0500
Subject: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is way off the microscopy topic, but unfortunately, it is relevant to
our daily activities of late.
I am trying to contact someone probably associated with this list to inform
them that they are infected with the SoBig virus and don't appear to know
it yet.

Like many of us, I have been receiving dozens of copies of the SoBig virus
of late. Being a curious type and being the IT guy for the computers in our
labs, I have been poking into some of the messages to see where they came
from. Even though the Return Path: and From: fields are usually spoofed, I
found that many were coming from a couple of sources. I have included the
relevant information below.

Since many of the alleged senders were frequent posters to this list I am
guessing that the real sender may be a member of this list. So if BILLPC on
SYMET.NET or PRIVAT on T-DIALIN.NET means something to one of you out
there, please see about getting your PC disinfected.

Thanks.

Warren

Received: from PRIVAT (pD9E7724F.dip.t-dialin.net [217.231.114.79])
by despam-3.iastate.edu (8.12.4/8.12.4) with ESMTP id h7QCNLKG007344
for {wesaia-at-iastate.edu} ; Tue, 26 Aug 2003 07:23:22 -0500
} From: {martin.voecks-at-web.de}


Received: from BILLPC (dsl1-216-90-9-53.symet.net [216.90.9.53])
by despam-2.iastate.edu (8.12.4/8.12.4) with ESMTP id h7REWjbX018529
for {wesaia-at-iastate.edu} ; Wed, 27 Aug 2003 09:32:46 -0500
Message-Id: {200308271432.h7REWjbX018529-at-despam-2.iastate.edu}
} From: {gary-at-gaugler.com}

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Wed Aug 27 12:55:50 2003



From: Chaoying Ni :      cni-at-udel.edu
Date: Wed, 27 Aug 2003 13:37:09 -0400 (EDT)
Subject: Re: TEM Analysis of HIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Dr. Ahmed,

We do have the capibiilty. What kind of arrangement would you propose to
have the work done?

****************************************
Chaoying Ni, PhD
The W.M. Keck Electron Microscope Facility
Facility location: 022 Spencer Laboratory
Mailing address:
201 duPont Hall
Dept of Matls Sci & Eng
University of Delaware
Newark, DE 19716
(302) 831-8354 (O); -2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************

On Wed, 27 Aug 2003, AHMED, TOSEEF wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} I am looking for a lab that can perform TEM analysis of high impact polystyrene. I intend to obtain an extended support for next few months in order to analyze 30-35 samples. The analysis involves specimen preparation (cryomicrotomy, staining), imaging at 2-3 reasonable magnifications and calculation of rubber volume fraction/size of each sample. I will appreciate the help of the list members in this regard. The interested labs on the list server may want to contact me of line for further discussions regarding the project.
}
} Best regards.
}
}
} Toseef Ahmed, Ph.D.
} Electron Microscopy and Thermal Labs, Analytical Section
} SABIC R&T, P.O. Box 42503, Riyadh 11551, Saudi Arabia
} Tel: (966-1) 265-3333 Ext.6003, 6104
} Fax: (966-1) 265-1101
} E-mail: toseef-at-sabic.com,
}
}


From daemon Wed Aug 27 14:45:01 2003



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 27 Aug 2003 15:21:13 -0400
Subject: Durst for 35mm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I have a Durst Laborator S-45 and want to do some 35mm work. I have the proper big glass lenses (130/85), the proper small lense (50mm).

I haven't do 35mm in a long time so I can't remember the complete setup. When I put in the 50mm lens in the turret and put the 130/85 in the proper configuration all I get is a lovely image of the filament of the light bulb that is the light source.

Does anybody know how to set this up right? I thought when I did this a looooong time ago that all I did was swap the big glass lenses and away I went.

Could it be that I don't have the correct light source in there? It's a smallish regular looking (by that I mean it looks like a bulb you use at home) clear glass light bulb.

Any suggestions are greatly appreciated. Of course I don't have/can't find the instruction manual that would to be convenient.


Dodging summer thunderstorms,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Electron Microscope Lab
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Wed Aug 27 16:52:43 2003



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 27 Aug 2003 17:46:16 -0400
Subject: RE: Poly-L-lysine: clarification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've found good success sticking live bacteria cells to the coverslips
FOR fixing and dehydrating...
Prep (slides or coverslips)
Clean slides in Ethanol with a small amount of dilute HCl
Rinse in Distilled water
Soak for 30 minutes in your Poly-L solution
Let air dry (can be stored desiccated for a long time)
The Solution that works well and as is published in Steven E. Ruzin's
Plant Microtechnique and Microscopy, calls for 100µg-1mg/ml and Tris
10mM, pH 8.0

I've had success with a "that’s about enough" diluting the stock Poly-L
with Distilled water (one or two mls per 100-200mls of dH20).

This has worked well for general histochemistry (wax sections) as well
as liquid suspended bacterial preparations for the SEM. Samples survive
fixation, washing, dehydration, and even CPD with significant coverage
where the Poly-L is.

We now get our Poly-L-Lysine from the big F chemical/supply company (the
S chemical company also has it) the 1% solution is what we get.

HTH

Geoff Williams
Microscopy Facility Supervisor

CMU Biology Department Microscopy Facility web page.
http://www.cst.cmich.edu/centers/microscopy/

} -----Original Message-----
} From: Patton, David [mailto:David.Patton-at-uwe.ac.uk]
} Sent: Wednesday, August 27, 2003 4:20 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Poly-L-lysine: clarification
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} My original post was ambiguous. I am looking for ways to
} stick fixed cells to coverslips to avoid repeated
} centrifugation during dehydration for SEM.
}
} Dave
}
}
} On Tue, 26 Aug 2003 15:11:46 +0100 (GMT Daylight Time)
} "Patton, David" {David.Patton-at-uwe.ac.uk} wrote:
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } I would like to try poly-L-lysine for sticking cells to
} } coverslips etc for dehydration and SEM. My S**** catalogue
} } offers about 15 variants. Can anyone advise me on which
} } type to use?
} }
} } Dave
} }
} } ----------------------------------------
} } Patton, David
} } Email: David.Patton-at-uwe.ac.uk
} } "University of the West of England"
} }
} }
} }
} }
} } This incoming email to UWE has been independently scanned for
viruses
} and any virus detected has been removed using McAfee anti-virus
software
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"




From daemon Wed Aug 27 18:24:46 2003



From: pkrsko-at-stevens-tech.edu ()
Date: Wed, 27 Aug 2003 18:16:24 -0500
Subject: Ask-A-Microscopist: TEM screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pkrsko-at-stevens-tech.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday, August 27, 2003 at 12:10:52
---------------------------------------------------------------------------

Email: pkrsko-at-stevens-tech.edu
Name: Peter Krsko

Organization: Stevens Inst Tech

Education: Graduate College

Location: Hoboken, NJ

Question: Hello,

I would like to find out if it's possible to get phosphorus used on TEM screens in solution. I am using simple electron beam mask and I want to highlight the apperture opening with this paint.

Your help is greatly appreciated.

Thank you.

Peter Krsko

P.S: Here are some images for you:
http://attila.stevens-tech.edu/~pkrsko/billboart/

---------------------------------------------------------------------------


From daemon Wed Aug 27 22:58:23 2003



From: shashi singh :      shashis_99-at-yahoo.com
Date: Wed, 27 Aug 2003 20:51:42 -0700 (PDT)
Subject: fixation of eye

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pedro,
Karnowsky's is the best. works both for LM and EM.
Shashi
CCMB
Hyderabad
INDIA

I'm looking for any feedback on fixation of eyes
(small and large
animal).
More specifically: a fixative that would work well for
Histology and
EM.
Any info. on:
* Glutaraldehyde
* Karnovsky's Fixative
* McDowell - Trump's Fixative
or any other fixative would be greatly appreciated.

Thanks in advance,

Pedro




__________________________________
Do you Yahoo!?
Yahoo! SiteBuilder - Free, easy-to-use web site design software
http://sitebuilder.yahoo.com


From daemon Wed Aug 27 23:23:59 2003



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 28 Aug 2003 00:17:44 -0500
Subject: TEM on HIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Toseef Ahmed, Ph.D. wrote:
=======================================================
I am looking for a lab that can perform TEM analysis of high impact
polystyrene. I intend to obtain an extended support for next few months in
order to analyze 30-35 samples. The analysis involves specimen preparation
(cryomicrotomy, staining), imaging at 2-3 reasonable magnifications and
calculation of rubber volume fraction/size of each sample. I will
appreciate the help of the list members in this regard. The interested labs
on the list server may want to contact me of line for further discussions
regarding the project.
=======================================================
There are a number of independent analytical laboratories in the USA and
elsewhere set up to do this type of TEM work for clients. We maintain a
listing of some of these laboratories on our website under our "Hot
Services" section, see URL
http://www.2spi.com/hot-service7.html

Structure Probe, Inc. has been conducting this type of characterization work
on high impact polystyrene (HIPS) since the inception of our business in
1970, both cryo and at RT. We are also accredited by A2LA to the standard
of ISO Guide 17025. We are not a university, but are a commercial business.
Providing a high standard of service to our clients is our #1 priority.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
Structure Probe, Inc. FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: www.2spi.com
############################
==================================================


From daemon Thu Aug 28 02:08:01 2003



From: Peter Van Osta :      pvosta-at-unionbio-eu.com
Date: Thu, 28 Aug 2003 09:24:46 +0200
Subject: Sample of 1536 or 6144 array or plate ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula
Sounds like you have the wrong bulb. An enlarger bulb is opal
(milky-white)
so the filament should not be visible, and the label should be printed
on the base
close to the cap, where it won't interfere with image formation .
Chris

Dr. Chris Jeffree
University of Edinburgh

----- Original Message -----
} From: "Paula Sicurello" {patpxs-at-gwumc.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 27, 2003 8:21 PM


Hi,

I am currently testing our new microscopy based reader on which I would
like to test a variety of samples. One of the samples I am looking for
is a plate which contains 6144 samples of cells or a tissue array within
the boundaries of a SBS-standard plate. Arrays or plates with 1536 well
or samples/spots on them would be useful too, if they fit into the
SBS-standard plate boundaries. The arrays should be in a evenly spaced
rectangular pattern. The carrier should be either glass or plastic.

Does anyone have such samples available which I could use for testing
either in brightfield mode or with fluorescence or a combination of both
? Preferably fixed material which can be reused several times for
testing.

Best regards,

Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)
Cipalstraat 3
B-2440 Geel
Belgium
Tel.: +32 (0)14 570 620
Fax.: +32 (0)14 570 621

http://www.unionbio.com/

http://ourworld.compuserve.com/homepages/pvosta/cvwww.htm


From daemon Thu Aug 28 08:27:27 2003



From: efosten-at-mmm.com
Date: Thu, 28 Aug 2003 08:21:24 -0500
Subject: M&M 2003 LAC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The list of Local Arrangements Committee members shown in the EXPO and
Proceedings for the 2003 Microscopy & Microanalysis meeting was based on a
preliminary list I submitted two months before the meeting for those
publications' deadlines. By the time of the meeting some of the people on
the list were unable to participate, and others not on the list attended
M&M 2003 and served on the LAC. To give the LAC members proper recognition
for the tremendous job they did, here are their names:

} From Texas:

Pamela Neill

Jodi Roepsch

Robert Champaign

Becky Holdford

Ann Burke

Josephine Taylor

Ann Rushing

Susan Robbins

Sandra Westmoreland

Robert Droleskey



} From Oklahoma:

Mary Sheldon

Elizabeth Duncan

Rae Harrington

Amy Sheldon



} From Minnesota:

Dwight Erickson, LAC Treasurer


Thanks to all!


Ev Osten

Local Arrangements Committee Chair
Microscopy & Microanalysis 2003
August 3 - 7, San Antonio

efosten-at-mmm.com
651-736-0104
fax: 651-733-0648

3M Company
Corporate Analytical Technology Center
3M Center, 201-BE-16
St. Paul, MN 55144-1000



From daemon Thu Aug 28 08:37:05 2003



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 28 Aug 2003 08:33:34 -0500
Subject: Durst for 35mm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

It sounds like you have the point-source bulb in there. The correct
bulb for that Durst is a large frosted bulb, the number of which I can
no longer remember. If your enlarger is set up for point source, you
will need to do a point-source alignment of the system and use the
proper condensers to get rid of the filament image. I no longer remember
how to do that, either, except that the lens must be used at its largest
f/stop. The point source lamp plugs into a rheostat to control the
brightness, instead of using regular f-stops, so if you switch to
regular condenser lighting, you will need to plug the lamp into a
regular outlet. If you later switch back to point-source you MUST
remember to switch back to the proper power source or the bulb will
explode like a little grenade and scare the *^%*%* out of you. Speaking
from experience here. :-)

Lots of luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core---We do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/



-----Original Message-----
} From: Paula Sicurello [mailto:patpxs-at-gwumc.edu]
Sent: Wednesday, August 27, 2003 2:21 PM
To: microscopy-at-sparc5.microscopy.com


Hi Listers,

I have a Durst Laborator S-45 and want to do some 35mm work. I have the
proper big glass lenses (130/85), the proper small lense (50mm).

I haven't do 35mm in a long time so I can't remember the complete setup.
When I put in the 50mm lens in the turret and put the 130/85 in the
proper configuration all I get is a lovely image of the filament of the
light bulb that is the light source.

Does anybody know how to set this up right? I thought when I did this a
looooong time ago that all I did was swap the big glass lenses and away
I went.

Could it be that I don't have the correct light source in there? It's a
smallish regular looking (by that I mean it looks like a bulb you use at
home) clear glass light bulb.

Any suggestions are greatly appreciated. Of course I don't have/can't
find the instruction manual that would to be convenient.


Dodging summer thunderstorms,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Electron Microscope Lab
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax




From daemon Thu Aug 28 08:53:04 2003



From: psconnel-at-sas.upenn.edu
Date: Thu, 28 Aug 2003 09:49:23 -0400
Subject: Re: Ask-A-Microscopist: Philips 200 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barb,
I think that I have everything that you would need. Please reply with what you
need in particular. I am currently using the Philips 200 and have complete
sets of the manuals and I think all the O-rings that you could possible need
too.
Pat Connelly
Research Specialist
Dept. of Biology
Univ. of Pennsylvania
Philadelphia, PA 19104-6018
215-898-7145


From daemon Thu Aug 28 09:02:15 2003



From: Materials Research Laboratories, Inc. :      mrllab-at-raex.com
Date: Thu, 28 Aug 2003 10:00:10 -0400
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Yet another reason to go with Macintosh! Not a problem on our end..

Carol Jean Hirt, VP
Materials Research Laboratories, Inc.
800-424-1776
www.mrllab.com

on 8/27/03 12:22 PM, Warren E Straszheim at wesaia-at-iastate.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This is way off the microscopy topic, but unfortunately, it is relevant to
} our daily activities of late.
} I am trying to contact someone probably associated with this list to inform
} them that they are infected with the SoBig virus and don't appear to know
} it yet.
}
} Like many of us, I have been receiving dozens of copies of the SoBig virus
} of late. Being a curious type and being the IT guy for the computers in our
} labs, I have been poking into some of the messages to see where they came
} from. Even though the Return Path: and From: fields are usually spoofed, I
} found that many were coming from a couple of sources. I have included the
} relevant information below.
}
} Since many of the alleged senders were frequent posters to this list I am
} guessing that the real sender may be a member of this list. So if BILLPC on
} SYMET.NET or PRIVAT on T-DIALIN.NET means something to one of you out
} there, please see about getting your PC disinfected.
}
} Thanks.
}
} Warren
}
} Received: from PRIVAT (pD9E7724F.dip.t-dialin.net [217.231.114.79])
} by despam-3.iastate.edu (8.12.4/8.12.4) with ESMTP id h7QCNLKG007344
} for {wesaia-at-iastate.edu} ; Tue, 26 Aug 2003 07:23:22 -0500
} } From: {martin.voecks-at-web.de}
}
}
} Received: from BILLPC (dsl1-216-90-9-53.symet.net [216.90.9.53])
} by despam-2.iastate.edu (8.12.4/8.12.4) with ESMTP id h7REWjbX018529
} for {wesaia-at-iastate.edu} ; Wed, 27 Aug 2003 09:32:46 -0500
} Message-Id: {200308271432.h7REWjbX018529-at-despam-2.iastate.edu}
} } From: {gary-at-gaugler.com}
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 46 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking
}
}
}
}



From daemon Thu Aug 28 10:14:40 2003



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Thu, 28 Aug 2003 10:08:11 -0500
Subject: Re: Poly-L-lysine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may wish to consider an alternative. The use of glass slides coated
with 2% aminopropyltriethoxysilane in dry acetone is now commonly used to
make sections adhere better. You dip once in 2% APTS for 10-30 sec, rinse
in acetone and then rinse in distilled water. It works far better than
poly-L-lysine or chrome-gel for this purpose. But it was originally
described by Gottlieb & Glaser (1976 Biochem Biophys Res Comm 63:815-821)
in conjunction with glutaraldehyde to get single cells to adhere to glass
better. You might want to check this paper out. good luck. tom



At 09:20 AM 8/27/2003 +0100, you wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Associate Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From daemon Thu Aug 28 11:30:48 2003



From: msteglic-at-mdanderson.org
Date: Thu, 28 Aug 2003 11:22:45 -0500
Subject: Durst for 35mm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




That is one cofiguration and should work OK. I have also used 240/200 and a 105
mm lens. If all you are getting is the filament image, then you need to adjust
the bellows and refocus on the grains in your film.
From the bulb description, it sounds like you are using a regular enlarging
bulb and not a point source. You can verify this by checking if the enlarger is
plugged directly into the timer or is there a variable reostat inbetween that
allows you to adjust the light intensity.
If you need further help in aligment, email me directly.

Mannie Steglich
Tech Dir E M Lab
U T M D Anderson Cancer Center
Houston TX
msteglic-at-mdanderson.org
---------------------- Forwarded by Mannie Steglich/MDACC on 08/28/2003 11:16 AM
---------------------------


Microscopy-request-at-sparc5.microscopy.com on 08/27/2003 02:21:13 PM

To: microscopy-at-sparc5.microscopy.com
cc:


Hi Listers,

I have a Durst Laborator S-45 and want to do some 35mm work. I have the proper
big glass lenses (130/85), the proper small lense (50mm).

I haven't do 35mm in a long time so I can't remember the complete setup. When I
put in the 50mm lens in the turret and put the 130/85 in the proper
configuration all I get is a lovely image of the filament of the light bulb that
is the light source.

Does anybody know how to set this up right? I thought when I did this a
looooong time ago that all I did was swap the big glass lenses and away I went.

Could it be that I don't have the correct light source in there? It's a
smallish regular looking (by that I mean it looks like a bulb you use at home)
clear glass light bulb.

Any suggestions are greatly appreciated. Of course I don't have/can't find the
instruction manual that would to be convenient.


Dodging summer thunderstorms,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Electron Microscope Lab
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax







From daemon Thu Aug 28 14:30:58 2003



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 28 Aug 2003 12:20:48 -0700 (PDT)
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Or better yet, go with Linux...

Or if you can't live without Windows - just get rid of outlook express on
every windows machine you have. Replace it with something better like
Eudora that doesn't have so many security flaws.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Thu, 28 Aug 2003, Materials Research Laboratories, Inc. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Yet another reason to go with Macintosh! Not a problem on our end..
}
} Carol Jean Hirt, VP
} Materials Research Laboratories, Inc.
} 800-424-1776
} www.mrllab.com
}
} on 8/27/03 12:22 PM, Warren E Straszheim at wesaia-at-iastate.edu wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } This is way off the microscopy topic, but unfortunately, it is relevant to
} } our daily activities of late.
} } I am trying to contact someone probably associated with this list to inform
} } them that they are infected with the SoBig virus and don't appear to know
} } it yet.
} }
} } Like many of us, I have been receiving dozens of copies of the SoBig virus
} } of late. Being a curious type and being the IT guy for the computers in our
} } labs, I have been poking into some of the messages to see where they came
} } from. Even though the Return Path: and From: fields are usually spoofed, I
} } found that many were coming from a couple of sources. I have included the
} } relevant information below.
} }
} } Since many of the alleged senders were frequent posters to this list I am
} } guessing that the real sender may be a member of this list. So if BILLPC on
} } SYMET.NET or PRIVAT on T-DIALIN.NET means something to one of you out
} } there, please see about getting your PC disinfected.
} }
} } Thanks.
} }
} } Warren
} }
} } Received: from PRIVAT (pD9E7724F.dip.t-dialin.net [217.231.114.79])
} } by despam-3.iastate.edu (8.12.4/8.12.4) with ESMTP id h7QCNLKG007344
} } for {wesaia-at-iastate.edu} ; Tue, 26 Aug 2003 07:23:22 -0500
} } } From: {martin.voecks-at-web.de}
} }
} }
} } Received: from BILLPC (dsl1-216-90-9-53.symet.net [216.90.9.53])
} } by despam-2.iastate.edu (8.12.4/8.12.4) with ESMTP id h7REWjbX018529
} } for {wesaia-at-iastate.edu} ; Wed, 27 Aug 2003 09:32:46 -0500
} } Message-Id: {200308271432.h7REWjbX018529-at-despam-2.iastate.edu}
} } } From: {gary-at-gaugler.com}
} }
} } -------------------------------------------
} } No files should be attached to this message
} } -------------------------------------------
} } Warren E. Straszheim, Ph.D.
} } Materials Analysis and Research Lab
} } Iowa State University
} } 46 Town Engineering
} } Ames IA, 50011-3232
} }
} } Ph: 515-294-8187
} } FAX: 515-294-4563
} }
} } E-Mail: wesaia-at-iastate.edu
} } Web: www.marl.iastate.edu
} }
} } Scanning electron microscopy, x-ray analysis, and image analysis of materials
} } Computer applications and networking
} }
} }
} }
} }
}
}


From daemon Thu Aug 28 15:35:32 2003



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 28 Aug 2003 15:30:39 -0500
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Going with Linux or Mac OS will probably ensure that you don't get infected
by a virus, but it won't spare you from receiving all the fool traffic that
these viruses have generated. These viruses are equal opportunity pains in
the butt in that regard.

But I agree with you, Windows is a high-profile target for virus writers.
Those of us using it cannot afford to let our guard down and say "it won't
happen to me". We should all have safeguards in place and make sure that
virus definitions are up to date if we are going to connect to the net.

Then there is also the often forgotten caution, don't open unexpected
attachments. I don't care how cute the clip or website is alleged to be, I
don't care to risk my computer over that. And if someone is sending me a
document, I want to have a clear indication that it is something worthwhile
and and authentic and safe. Messages supposedly from colleagues that say
simply "Here is the new file - check it out" don't do much to certify that
it is a file that I should trust.

BTW, Gordon, one of the virus copies was _allegedly_ from you.

You all be careful out there.
Warren

At 12:20 PM 8/28/2003 -0700, you wrote:

} Or better yet, go with Linux...
}
} Or if you can't live without Windows - just get rid of outlook express on
} every windows machine you have. Replace it with something better like
} Eudora that doesn't have so many security flaws.
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
} On Thu, 28 Aug 2003, Materials Research Laboratories, Inc. wrote:
} }
} } Yet another reason to go with Macintosh! Not a problem on our end..
} }
} } Carol Jean Hirt, VP
} } Materials Research Laboratories, Inc.
} } 800-424-1776
} } www.mrllab.com

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Thu Aug 28 16:16:30 2003



From: John Perrino :      jperrino-at-stanford.edu
Date: Thu, 28 Aug 2003 14:12:57 -0700
Subject: Re: Durst 35mm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,
The Durst 45-S should have the two condensor lens configurations printed
out on the cover, front panel, of the enlarger head. Some will be for 4x5,
120, and 35mm. Unfortunately I never did much work for 35mm since we had an
easy to use Omega with the frosted bulb. A point-source bulb, which is what
you have, will give you better images but may also exacerbate problems you may
have on the film, such as dust and scratches, which is why many people opt for
the frosted set-up, especially for 35mm. Good luck
John

--
John Perrino
Cell Sciences Imaging Facility
Beckman B001
Stanford University
Stanford, CA 94305
650-723-3462


From daemon Thu Aug 28 16:41:25 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 28 Aug 2003 14:36:30 -0700
Subject: Re: Durst for 35mm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,
It's not true, halogen bulbs in relatively modern enlargers are
transparent. Halogen bulbs delivered much more "light" and it's spectral
composition is better for color photography, which, actually, unimportant
for B&W. The important thing is that those bulbs considered as a "pointed
light source", so the image is sharper and more contrasty.

In my old Devere enlarger, I am using the following combinations:

Condenser: top lens (mm) 180 180 120
bottom 180 120 100
Objective lens------------} 150-105 105-75 60-50 mm

The bulb is transparent, but I am not quite sure it's halogen.

Unfortunately, I know nothing about Durst enlargers.

Sergey



At 12:01 AM 8/28/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Aug 28 17:10:09 2003



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 28 Aug 2003 15:05:30 -0700 (PDT)
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Virus from me...?
I doubt it. Send me the full headers and I'll check it out. My email is
run through pine on a SunOS computer.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Thu, 28 Aug 2003, Warren E Straszheim wrote:

} Going with Linux or Mac OS will probably ensure that you don't get infected
} by a virus, but it won't spare you from receiving all the fool traffic that
} these viruses have generated. These viruses are equal opportunity pains in
} the butt in that regard.
}
} But I agree with you, Windows is a high-profile target for virus writers.
} Those of us using it cannot afford to let our guard down and say "it won't
} happen to me". We should all have safeguards in place and make sure that
} virus definitions are up to date if we are going to connect to the net.
}
} Then there is also the often forgotten caution, don't open unexpected
} attachments. I don't care how cute the clip or website is alleged to be, I
} don't care to risk my computer over that. And if someone is sending me a
} document, I want to have a clear indication that it is something worthwhile
} and and authentic and safe. Messages supposedly from colleagues that say
} simply "Here is the new file - check it out" don't do much to certify that
} it is a file that I should trust.
}
} BTW, Gordon, one of the virus copies was _allegedly_ from you.
}
} You all be careful out there.
} Warren
}
} At 12:20 PM 8/28/2003 -0700, you wrote:
}
} } Or better yet, go with Linux...
} }
} } Or if you can't live without Windows - just get rid of outlook express on
} } every windows machine you have. Replace it with something better like
} } Eudora that doesn't have so many security flaws.
} }
} } \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} } Gordon Ante Vrdoljak Electron Microscope Lab
} } ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} } gvrdolja-at-nature.berkeley.edu UC Berkeley
} } phone (510) 642-2085 Berkeley CA 94720-3330
} } fax (510) 643-6207 cell (510) 290-6793
} }
} } On Thu, 28 Aug 2003, Materials Research Laboratories, Inc. wrote:
} } }
} } } Yet another reason to go with Macintosh! Not a problem on our end..
} } }
} } } Carol Jean Hirt, VP
} } } Materials Research Laboratories, Inc.
} } } 800-424-1776
} } } www.mrllab.com
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 46 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking
}
}


From daemon Thu Aug 28 19:07:26 2003



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 29 Aug 2003 10:06:35 +1000
Subject: lens epithelium - fixing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I've been asked to see what the EM difference is between rat eye lens
epithelium in culture and in situ. So I'd like to fix the epithelium
while it is still in the eye. I'm worried that if I do that it then
won't be possible to get the epithelium off the lens without damaging
it; I also seem to remember that lens becomes brittle and difficult
to handle when fixed. I'd like to keep life simple, so it may be
necessary to take the epithelium off the lens before fixing. Does
anyone have experience with this?

Thanks,

Diana


From daemon Thu Aug 28 20:51:55 2003



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 28 Aug 2003 18:47:44 -0700
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Perhaps this is a good time to engage PDF
as safe attachments(?). It would seem so.

The Mac is not particularly affected since it
constitutes a small percentage of PC sales
and total installed base. However, it is not immune.
It is just that nobody wastes time on a minuscule
platform. If it is hyped-up, of course that
may change.

gary g.


At 01:30 PM 8/28/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Aug 28 22:20:28 2003



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 29 Aug 2003 13:21:27 +1000
Subject: Re: eye fixatives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The main thing with fixing eyes is to make sure that the fix can get
in - so slit across the cornea and take out the lens (assuming you
don't want either of these). For EM, glut is OK, but because an eye
is big, the fast penetration/fixation of paraform combined with the
thorough action of glut is better - so Karnovsky's. If you need to do
immuno, neither of these is suitable; PLP (paraform/lysine/periodate)
is good or just paraform. As for McD-Trump - I've never heard of it!

Diana

At 12:19 PM -0400 26/8/03, Louro, Pedro wrote:
} I'm looking for any feedback on fixation of eyes (small and large animal).
} More specifically: a fixative that would work well for Histology and EM.
} Any info. on:
} * Glutaraldehyde
} * Karnovsky's Fixative
} * McDowell - Trump's Fixative
} or any other fixative would be greatly appreciated.
}
} Thanks in advance,
}
} Pedro

--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Fri Aug 29 03:56:06 2003



From: sstouden-at-thelinks.com
Date: Thu, 28 Aug 2003 20:45:36 -0500 (CDT)
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



My linux server has not noticed, is there a new virus ?

but my windows box cannot find time to shut out the viri, the popups, the
the cookies and the adware, spyware, and other stuff. Could it be because
some of the browsers were written to accomodate, to force the user to put
up with these things? Browsers simply read the file that are transmitted
from the server to the client. If the browsers ignored the adware, the
pop ups and stuff, there would be few people sending them out, because no
one could read them.

What is needed is concerted organized effort by those of us in the
Internet community to find the people that entered this virus into the
system.

Interesting thought, back in 1947, 48, and 49 there was a similar problem
with people on the radio, everybody kept trying to out maneuver the next
guy so that no one could get anything on their personal radio receivers.

It turned out that the big stations were heavily financed and they wanted
the little guys (competition) off the air. So they thought, we will keep
doing this until we can get the government to license the airways, in
that manner we can control the media called radio by forcing them to
get licensed, and by limiting the number of licenses, and eliminate our
competition, since the little guys do not have money, manpower, or
knowledge about how to lobby the government.

Now this is not a result we want to happen with the internet. Government
should not be involved in the email system. So let's start finding the
persons that did this So Big Virus. Lets enlist everyone in the net to
help back track it. until we find its source. That should not be too big
of a job. it only happened last week.


I am betting the guys responsible for it, would suprise us all?

Any ideas how we could back trace this?
one idea is two create two threads on this list:
1. to identify So Big by its entry date and source amoung members
2. the other to report any progress in backtracking to the origin
that is known or reported by anyone anywhere through out the
world as one or more of us becomes aware of it.

sterling


On Thu, 28 Aug 2003, Warren E Straszheim wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Going with Linux or Mac OS will probably ensure that you don't get infected
} by a virus, but it won't spare you from receiving all the fool traffic that
} these viruses have generated. These viruses are equal opportunity pains in
} the butt in that regard.
}
} But I agree with you, Windows is a high-profile target for virus writers.
} Those of us using it cannot afford to let our guard down and say "it won't
} happen to me". We should all have safeguards in place and make sure that
} virus definitions are up to date if we are going to connect to the net.
}
} Then there is also the often forgotten caution, don't open unexpected
} attachments. I don't care how cute the clip or website is alleged to be, I
} don't care to risk my computer over that. And if someone is sending me a
} document, I want to have a clear indication that it is something worthwhile
} and and authentic and safe. Messages supposedly from colleagues that say
} simply "Here is the new file - check it out" don't do much to certify that
} it is a file that I should trust.
}
} BTW, Gordon, one of the virus copies was _allegedly_ from you.
}
} You all be careful out there.
} Warren
}
} At 12:20 PM 8/28/2003 -0700, you wrote:
}
} } Or better yet, go with Linux...
} }
} } Or if you can't live without Windows - just get rid of outlook express on
} } every windows machine you have. Replace it with something better like
} } Eudora that doesn't have so many security flaws.
} }
} } \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} } Gordon Ante Vrdoljak Electron Microscope Lab
} } ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} } gvrdolja-at-nature.berkeley.edu UC Berkeley
} } phone (510) 642-2085 Berkeley CA 94720-3330
} } fax (510) 643-6207 cell (510) 290-6793
} }
} } On Thu, 28 Aug 2003, Materials Research Laboratories, Inc. wrote:
} } }
} } } Yet another reason to go with Macintosh! Not a problem on our end..
} } }
} } } Carol Jean Hirt, VP
} } } Materials Research Laboratories, Inc.
} } } 800-424-1776
} } } www.mrllab.com
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 46 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking
}
}
}



From daemon Fri Aug 29 06:02:03 2003



From: leeb-at-uow.edu.au
Date: Fri, 29 Aug 2003 20:55:49 +1000
Subject: TEM/UMT-wish to locate DiaTech knife-maker or successor company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know what has become of DiaTech in Knoxville
Tenn.? They were makers of fine diamond knives for
ultramicrotomy and must have folded or been sold on to
another company--any news of them? Many thanks,
Lee F. Brunckhorst leeb-at-uow.edu.au
(Wollongong University Materials Engineering, Australia)



From daemon Fri Aug 29 06:40:07 2003



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Fri, 29 Aug 2003 08:35:06 -0300
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'll have to go with Gary on this one, at the risk of starting
a flame war. My Timex Sinclair and Atari ST are completely
immune to viruses for the same reason...

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Fri Aug 29 07:33:49 2003



From: Michael Cheatham :      mmcheath-at-mailbox.syr.edu
Date: Fri, 29 Aug 2003 08:22:30 -0400
Subject: EBSD/SEM: Sample Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
Could those of you doing EBSD in an SEM kindly share your
sample prep method with me. I welcome all feedback, but would
particularly like to here from those working with geological samples.
Is anyone using an Ion Mill? If so, what is the maximum size of the
'thinned area'?

TIA
Mike
--
********************************************************************
Michael M. Cheatham
312 Heroy Geology Laboratory Phone (315)-443-1261
Syracuse University Fax (315)-443-3363
Syracuse, NY 13244-1070

email:mmcheath-at-mailbox.syr.edu
http://www.geochemistry.syr.edu/cheatham/InstrPages.html

owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html
owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html
owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html
owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html
********************************************************************


From daemon Fri Aug 29 09:03:20 2003



From: Ann-Fook Yang :      yanga-at-agr.gc.ca
Date: Fri, 29 Aug 2003 09:58:10 -0400
Subject: Durst for 35mm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

Mannie is correct in suggesting to adjust the bellow to focus the grain
on your negative. Specifically, you should bring the 50mm lens closer to
the condensers. You were at a wrong focal plane when you see filament.
It does not matter what bulb you use in terms of focusing.

Ann Fook Yang

} } } {"msteglic-at-mdanderson.org"-at-sparc5.microscopy.com} 08/28/03 12:22PM
} } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America




That is one cofiguration and should work OK. I have also used 240/200
and a 105
mm lens. If all you are getting is the filament image, then you need to
adjust
the bellows and refocus on the grains in your film.
From the bulb description, it sounds like you are using a regular
enlarging
bulb and not a point source. You can verify this by checking if the
enlarger is
plugged directly into the timer or is there a variable reostat
inbetween that
allows you to adjust the light intensity.
If you need further help in aligment, email me directly.

Mannie Steglich
Tech Dir E M Lab
U T M D Anderson Cancer Center
Houston TX
msteglic-at-mdanderson.org
---------------------- Forwarded by Mannie Steglich/MDACC on 08/28/2003
11:16 AM
---------------------------


Microscopy-request-at-sparc5.microscopy.com on 08/27/2003 02:21:13 PM

To: microscopy-at-sparc5.microscopy.com
cc:


Hi Listers,

I have a Durst Laborator S-45 and want to do some 35mm work. I have
the proper
big glass lenses (130/85), the proper small lense (50mm).

I haven't do 35mm in a long time so I can't remember the complete
setup. When I
put in the 50mm lens in the turret and put the 130/85 in the proper
configuration all I get is a lovely image of the filament of the light
bulb that
is the light source.

Does anybody know how to set this up right? I thought when I did this
a
looooong time ago that all I did was swap the big glass lenses and away
I went.

Could it be that I don't have the correct light source in there? It's
a
smallish regular looking (by that I mean it looks like a bulb you use
at home)
clear glass light bulb.

Any suggestions are greatly appreciated. Of course I don't have/can't
find the
instruction manual that would to be convenient.


Dodging summer thunderstorms,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Electron Microscope Lab
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax








From daemon Fri Aug 29 09:17:23 2003



From: Ann-Fook Yang :      yanga-at-agr.gc.ca
Date: Fri, 29 Aug 2003 10:14:06 -0400
Subject: Durst for 35mm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

Mannie is correct in suggesting to adjust the bellow to focus the grain
on your negative. Specifically, you should bring the 50mm lens closer to
the condensers. You were at a wrong focal plane when you see filament.
It does not matter what bulb you use in terms of focusing.

Ann Fook Yang

} } } {"msteglic-at-mdanderson.org"-at-sparc5.microscopy.com} 08/28/03 12:22PM
} } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America




That is one cofiguration and should work OK. I have also used 240/200
and a 105
mm lens. If all you are getting is the filament image, then you need to
adjust
the bellows and refocus on the grains in your film.
From the bulb description, it sounds like you are using a regular
enlarging
bulb and not a point source. You can verify this by checking if the
enlarger is
plugged directly into the timer or is there a variable reostat
inbetween that
allows you to adjust the light intensity.
If you need further help in aligment, email me directly.

Mannie Steglich
Tech Dir E M Lab
U T M D Anderson Cancer Center
Houston TX
msteglic-at-mdanderson.org
---------------------- Forwarded by Mannie Steglich/MDACC on 08/28/2003
11:16 AM
---------------------------


Microscopy-request-at-sparc5.microscopy.com on 08/27/2003 02:21:13 PM

To: microscopy-at-sparc5.microscopy.com
cc:


Hi Listers,

I have a Durst Laborator S-45 and want to do some 35mm work. I have
the proper
big glass lenses (130/85), the proper small lense (50mm).

I haven't do 35mm in a long time so I can't remember the complete
setup. When I
put in the 50mm lens in the turret and put the 130/85 in the proper
configuration all I get is a lovely image of the filament of the light
bulb that
is the light source.

Does anybody know how to set this up right? I thought when I did this
a
looooong time ago that all I did was swap the big glass lenses and away
I went.

Could it be that I don't have the correct light source in there? It's
a
smallish regular looking (by that I mean it looks like a bulb you use
at home)
clear glass light bulb.

Any suggestions are greatly appreciated. Of course I don't have/can't
find the
instruction manual that would to be convenient.


Dodging summer thunderstorms,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Electron Microscope Lab
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax








From daemon Fri Aug 29 09:30:36 2003



From: sstouden-at-thelinks.com
Date: Fri, 29 Aug 2003 02:23:10 -0500 (CDT)
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am glad you mentioned PDF, i have noticed that when I load some PDf's
that all of a sudden my computer starts sending things outbound lots and
lots of bits and I cannot stop it unless I shut down and reboot. anyone
else notice that with PDF?
Can someone say what it is that is happening?

sterling


On Thu, 28 Aug 2003, Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Perhaps this is a good time to engage PDF
} as safe attachments(?). It would seem so.
}
} The Mac is not particularly affected since it
} constitutes a small percentage of PC sales
} and total installed base. However, it is not immune.
} It is just that nobody wastes time on a minuscule
} platform. If it is hyped-up, of course that
} may change.
}
} gary g.
}
}
} At 01:30 PM 8/28/2003, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Going with Linux or Mac OS will probably ensure that you don't get
} } infected by a virus, but it won't spare you from receiving all the fool
} } traffic that these viruses have generated. These viruses are equal
} } opportunity pains in the butt in that regard.
} }
} } But I agree with you, Windows is a high-profile target for virus writers.
} } Those of us using it cannot afford to let our guard down and say "it won't
} } happen to me". We should all have safeguards in place and make sure that
} } virus definitions are up to date if we are going to connect to the net.
} }
} } Then there is also the often forgotten caution, don't open unexpected
} } attachments. I don't care how cute the clip or website is alleged to be, I
} } don't care to risk my computer over that. And if someone is sending me a
} } document, I want to have a clear indication that it is something
} } worthwhile and and authentic and safe. Messages supposedly from colleagues
} } that say simply "Here is the new file - check it out" don't do much to
} } certify that it is a file that I should trust.
} }
} } BTW, Gordon, one of the virus copies was _allegedly_ from you.
} }
} } You all be careful out there.
} } Warren
} }
} } At 12:20 PM 8/28/2003 -0700, you wrote:
} }
} } } Or better yet, go with Linux...
} } }
} } } Or if you can't live without Windows - just get rid of outlook express on
} } } every windows machine you have. Replace it with something better like
} } } Eudora that doesn't have so many security flaws.
} } }
} } } \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} } } Gordon Ante Vrdoljak Electron Microscope Lab
} } } ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} } } gvrdolja-at-nature.berkeley.edu UC Berkeley
} } } phone (510) 642-2085 Berkeley CA 94720-3330
} } } fax (510) 643-6207 cell (510) 290-6793
} } }
} } } On Thu, 28 Aug 2003, Materials Research Laboratories, Inc. wrote:
} } } }
} } } } Yet another reason to go with Macintosh! Not a problem on our end..
} } } }
} } } } Carol Jean Hirt, VP
} } } } Materials Research Laboratories, Inc.
} } } } 800-424-1776
} } } } www.mrllab.com
} }
} } -------------------------------------------
} } No files should be attached to this message
} } -------------------------------------------
} } Warren E. Straszheim, Ph.D.
} } Materials Analysis and Research Lab
} } Iowa State University
} } 46 Town Engineering
} } Ames IA, 50011-3232
} }
} } Ph: 515-294-8187
} } FAX: 515-294-4563
} }
} } E-Mail: wesaia-at-iastate.edu
} } Web: www.marl.iastate.edu
} }
} } Scanning electron microscopy, x-ray analysis, and image analysis of materials
} } Computer applications and networking
} }
}
}



From daemon Fri Aug 29 09:34:34 2003



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Fri, 29 Aug 2003 10:31:14 -0400
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Perhaps this is a good time to engage PDF
} as safe attachments(?). It would seem so.
}
} The Mac is not particularly affected since it
} constitutes a small percentage of PC sales
} and total installed base. However, it is not immune.
} It is just that nobody wastes time on a minuscule
} platform. If it is hyped-up, of course that
} may change.
}
} gary g.

Actually also not a good idea, there are such things as a PDF virus.

The biggest problem with Windows (any flavor) is Outlook Express. As
installed, it auto executes any Attachment. This makes it a sucker
punch for the common cracker. If Windows users turned that "feature"
off or use a different email app (like Eudora) then virus propagation
would drop by many factors. This is how the SoBig virus infects and
propagates.

Another problem is Windows (any flavor), by default, has many network
ports open that have no business being open thus exposing the machine
to numerous buffer overflow cracks. This is how the MSBlaster virus
infects and propagates.

As for the MacOS not being affected because the size of it's market.
A small gain of truth but mostly FUD. Market share of linux is also
small but that does not keep the virus and cracks away. Also
statistically, even though the MacOS is smaller, one should see a few
virus problems over the years. What do I see, zero, nothing. I don't
even run virus protection software on the MacOS any more. Stopped
doing that many years ago.

As a professional programmer fluent in apps and drivers in both the
WinOS and MacOS world, the truth is there are fundamental differences
in the way OS9 works from Windows that make 95% of the virus cracks
impossible to implement. Can you design a virus to infect MacOS9, yes
but you are going to have to work really hard at it. Script kiddies
and hack virus programmers need not apply.

OSX is also different but OSX is an industrial strength OS that is
installed in a safe manner (for example, there are no network ports
open unless you open them. Note that does not mean you can't surf the
net, it means that no one can surf you). Also Apple takes a defensive
approach, they assume that there are evil people out there that want
to mess with your computer. Microsoft does not design Windows with
security in mind, they keep saying they are but as long as those
extraneous network ports are open by default, I know they are not.

As a case in point, 4pi runs an powerpc linux server for web, ftp and
mail services. We always get probed and I mean seriously probed, but
they can't get in because 1) it's a powerpc box and intel linux
cracks don't work. and 2) it's locked down with regard to security.
We have all flavors of OSs here. Macs (both OS9 and OSX) and linux
boxes are permitted email access. Windows boxes are not. Too much
risk with cracks. Last time I saw a virus problem on a MacOS box was
back in the pre-powerpc days and that was an programming experiment
in self infection.

For more years, than I can count, non-MacOS users have been declaring
victory and Apple with the MacOS dead because of its size. Apple is
still around, Macs are still around and with OSX, the market size is
even growing. OSX is pretty neat I must say.

And last, you will not keep out a professional cracker out of your
computer in the same way you will not keep a professional thief out
of your house. Professional are a different breed, they will get in.
That's way they are called professionals. Thankfully, most all virus
and crack writers are not real professionals. I can recommend
several books that will put the fear of god in anyone that thinks the
internet is a benign place. It's a real war zone and the sooner we
accept that fact and convince companies such as Microsoft to default
the OS in a secure mode, then we will start to see a reduction rather
than a increase in cracks.

Scott




From daemon Fri Aug 29 12:55:33 2003



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 29 Aug 2003 10:48:55 -0700 (PDT)
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microsoft resorted to linux to protect its servers. I found it funny.
http://www.internetweek.com/story/showArticle.jhtml?articleID=13100775


\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Thu, 28 Aug 2003, Gordon Vrololjak wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Or better yet, go with Linux...
}
} Or if you can't live without Windows - just get rid of outlook express on
} every windows machine you have. Replace it with something better like
} Eudora that doesn't have so many security flaws.
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
} On Thu, 28 Aug 2003, Materials Research Laboratories, Inc. wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } Yet another reason to go with Macintosh! Not a problem on our end..
} }
} } Carol Jean Hirt, VP
} } Materials Research Laboratories, Inc.
} } 800-424-1776
} } www.mrllab.com
} }
} } on 8/27/03 12:22 PM, Warren E Straszheim at wesaia-at-iastate.edu wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.msa.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } This is way off the microscopy topic, but unfortunately, it is relevant to
} } } our daily activities of late.
} } } I am trying to contact someone probably associated with this list to inform
} } } them that they are infected with the SoBig virus and don't appear to know
} } } it yet.
} } }
} } } Like many of us, I have been receiving dozens of copies of the SoBig virus
} } } of late. Being a curious type and being the IT guy for the computers in our
} } } labs, I have been poking into some of the messages to see where they came
} } } from. Even though the Return Path: and From: fields are usually spoofed, I
} } } found that many were coming from a couple of sources. I have included the
} } } relevant information below.
} } }
} } } Since many of the alleged senders were frequent posters to this list I am
} } } guessing that the real sender may be a member of this list. So if BILLPC on
} } } SYMET.NET or PRIVAT on T-DIALIN.NET means something to one of you out
} } } there, please see about getting your PC disinfected.
} } }
} } } Thanks.
} } }
} } } Warren
} } }
} } } Received: from PRIVAT (pD9E7724F.dip.t-dialin.net [217.231.114.79])
} } } by despam-3.iastate.edu (8.12.4/8.12.4) with ESMTP id h7QCNLKG007344
} } } for {wesaia-at-iastate.edu} ; Tue, 26 Aug 2003 07:23:22 -0500
} } } } From: {martin.voecks-at-web.de}
} } }
} } }
} } } Received: from BILLPC (dsl1-216-90-9-53.symet.net [216.90.9.53])
} } } by despam-2.iastate.edu (8.12.4/8.12.4) with ESMTP id h7REWjbX018529
} } } for {wesaia-at-iastate.edu} ; Wed, 27 Aug 2003 09:32:46 -0500
} } } Message-Id: {200308271432.h7REWjbX018529-at-despam-2.iastate.edu}
} } } } From: {gary-at-gaugler.com}
} } }
} } } -------------------------------------------
} } } No files should be attached to this message
} } } -------------------------------------------
} } } Warren E. Straszheim, Ph.D.
} } } Materials Analysis and Research Lab
} } } Iowa State University
} } } 46 Town Engineering
} } } Ames IA, 50011-3232
} } }
} } } Ph: 515-294-8187
} } } FAX: 515-294-4563
} } }
} } } E-Mail: wesaia-at-iastate.edu
} } } Web: www.marl.iastate.edu
} } }
} } } Scanning electron microscopy, x-ray analysis, and image analysis of materials
} } } Computer applications and networking
} } }
} } }
} } }
} } }
} }
} }
}


From daemon Fri Aug 29 13:06:09 2003



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Fri, 29 Aug 2003 14:02:24 -0400
Subject: Re: Durst for 35mm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

What you describe sounds like you are mounting the 50mm lens below the lens
mounting turret in the same manner as you mount the 135mm and 150mm lenses
for larger format negatives.

The 50mm lens must be recessed into the bellows. There is a tube shaped
mounting adapter that fits into the lens mounting turret.

Frank
****************************************************************************
Frank Macaluso tel: 718-430-3547
Analytical Imaging Facility fax: 718-430-8996
Albert Einstein College of Medicine e-mail: macaluso-at-aecom.yu.edu
1300 Morris Park Avenue http://www.aecom.yu.edu/aif/
Bronx, NY 10461
****************************************************************************



From daemon Fri Aug 29 13:07:22 2003



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Fri, 29 Aug 2003 13:03:45 -0500
Subject: Administrivia: End Thread on Off-Topic: SoBig.....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

I believe this thread has had a more than sufficient run.
It's time to get back to Microscopy & Microanalysis.


Nestor
Your Friendly Neighborhood SysOp
--
===========================================

The box said ...
"This program requires Win 95/98/NT/XP or better..."
So I bought a G3 Mac !

===========================================


From daemon Fri Aug 29 13:32:15 2003



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 29 Aug 2003 13:32:58 -0500
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No, the virus was not from you. I said "_allegedly_" from you. You can
check out the headers below. The culprit was BILLPC, whoever that may be.
Your name (and many other listers including myself and Debby Sherman, etc)
have been forged as the sources. I just wanted you to know you were
included in the party. {G}

More viruses are hiding or forging the identity of the true sender. I don't
assume anymore that the alleged source is the real source but instead
examine the other headers before I try to alert someone to an infection on
their end. I have been scolded as the source of a virus on several such
occasions. In all cases, so far, I have not been the real source.

Warren

At 03:05 PM 8/28/2003 -0700, Gordon V. wrote:
} Virus from me...?
} I doubt it. Send me the full headers and I'll check it out. My email is
} run through pine on a SunOS computer.

Received: from BILLPC (dsl1-216-90-9-53.symet.net [216.90.9.53])
by despam-2.iastate.edu (8.12.4/8.12.4) with ESMTP id h7RKJFbX022026
for {wesaia-at-iastate.edu} ; Wed, 27 Aug 2003 15:19:17 -0500
Message-Id: {200308272019.h7RKJFbX022026-at-despam-2.iastate.edu}
} From: {gvrdolja-at-nature.berkeley.edu}
To: {wesaia-at-iastate.edu}


I suppose it could be one of a couple things with PDF files.

First, I think PDF files may now allow for some code, either macros or
embedded files, that could execute on your computer when you view the file.
I would need a real PDF expert to comment on the current capabilities. I
know MS Word document files were immune from infection until Word 97, IIRC.
About that time, MS started supporting macros as valid parts of Word
documents. It wasn't long before hackers started exploiting the "feature".
Even before that, the Concept virus came out using a document template
(.DOT) named as a document (.DOC) showing that documents could now be hosts
for viruses. Maybe the same thing is happening to PDF files.

Second, it could be that PDF is not the real file extension. I know I have
seen attachments with names such as "details.txt.vbs". The file could show
up as "details.txt" in the mail window or an Explorer window if the system
was set to hide known file extensions. Unfortunately, the default Windows
installation hides extensions, ala Mac, and allows hackers to use this
trick to fool users into thinking the file is just a benign text or PDF
file. But when the file is executed, Windows uses the true, hidden
extension to determine what to do with the file.

Either way, I come back to the conclusion that attachments should be
received only cautiously. I appreciate Scott Davilla's comments that the
Internet is a very dangerous place. If we knew just how dangerous, we might
be more careful.

Warren

At 02:23 AM 8/29/2003 -0500, you wrote:

} I am glad you mentioned PDF, i have noticed that when I load some PDf's
} that all of a sudden my computer starts sending things outbound lots and
} lots of bits and I cannot stop it unless I shut down and reboot. anyone
} else notice that with PDF?
} Can someone say what it is that is happening?
}
} sterling

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking




From daemon Fri Aug 29 14:35:30 2003



From: John Perrino :      jperrino-at-stanford.edu
Date: Fri, 29 Aug 2003 12:29:45 -0700
Subject: Nebulizer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
I am looking into getting a nebulizer to deposit proteins in glycerol
as 25-150um diameter droplets onto cleaved sheets of mica that will later
be rotary shadowed. I have heard that the bulb-driven nebulizers do not
give enough condensed force of spray to create fine droplets. Does anyone
have experience with this method and can suggest protocols and a nebulizer
to purchase?
John
--
John Perrino
Cell Sciences Imaging Facility
Beckman B001
Stanford University
Stanford, CA 94305
650-723-3462


From daemon Fri Aug 29 17:09:35 2003



From: Iain FIELDEN(MRI) :      I.Fielden-at-shu.ac.uk
Date: Fri, 29 Aug 2003 23:02:45 +0100
Subject: SEM ESEM Converter plates

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Does anyone use converter plates for backscattered imaging?

If you do, or if you know someone who does, I'd be interested
in knowing what you use them for and why.

Am I right in thinking that they are never used nowadays and
that they fell out of use as a result of cheap semi-conductor
detectors.

Regards,
Iain Fielden
---
Iain Fielden BEng AMInstP - Researcher
Materials Research Institute
Norfolk Building, Sheffield Hallam University
Howard Street, Sheffield, S1 1WB U.K.
Phone (Direct) + 44 (0)114 225 4081 Fax + 44 (0)114 225 3501
I.Fielden-at-shu.ac.uk SHU switchboard + 44 (0)114 225 5555
http://materials.shu.ac.uk/ http://www.shu.ac.uk/



From daemon Fri Aug 29 17:32:19 2003



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 29 Aug 2003 17:28:42 -0500
Subject: GFP antibody

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Can anyone recommend a source for an antibody against rabbit-GFP to use
for TEM immunocytochemistry? We are trying to localize a protein with a GFP
construct in arabidopsis root tissue. We have tried one antibody but
labeling is poor and not well localized. We want to make sure that it is
not the antibody. The protein is an unknown and the research group has not
yet been able to develop a clean antibody against it.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907



From daemon Fri Aug 29 20:40:47 2003



From: sstouden-at-thelinks.com
Date: Fri, 29 Aug 2003 13:29:19 -0500 (CDT)
Subject: Re: Off-topic SoBig virus victim

Contents Retrieved from Microscopy Listserver Archives
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Apparently they may have found him
http://www.reuters.com/newsArticle.jhtml;jsessionid=1MBT2YBXGTM1QCRBAELCFEY?type=topNews&storyID=3361073

On Thu, 28 Aug 2003 sstouden-at-thelinks.com wrote:

}
} My linux server has not noticed, is there a new virus ?
}
} but my windows box cannot find time to shut out the viri, the popups, the
} the cookies and the adware, spyware, and other stuff. Could it be because
} some of the browsers were written to accomodate, to force the user to put
} up with these things? Browsers simply read the file that are transmitted
} from the server to the client. If the browsers ignored the adware, the
} pop ups and stuff, there would be few people sending them out, because no
} one could read them.
}
} What is needed is concerted organized effort by those of us in the
} Internet community to find the people that entered this virus into the
} system.
}
} Interesting thought, back in 1947, 48, and 49 there was a similar problem
} with people on the radio, everybody kept trying to out maneuver the next
} guy so that no one could get anything on their personal radio receivers.
}
} It turned out that the big stations were heavily financed and they wanted
} the little guys (competition) off the air. So they thought, we will keep
} doing this until we can get the government to license the airways, in
} that manner we can control the media called radio by forcing them to
} get licensed, and by limiting the number of licenses, and eliminate our
} competition, since the little guys do not have money, manpower, or
} knowledge about how to lobby the government.
}
} Now this is not a result we want to happen with the internet. Government
} should not be involved in the email system. So let's start finding the
} persons that did this So Big Virus. Lets enlist everyone in the net to
} help back track it. until we find its source. That should not be too big
} of a job. it only happened last week.
}
}
} I am betting the guys responsible for it, would suprise us all?
}
} Any ideas how we could back trace this?
} one idea is two create two threads on this list:
} 1. to identify So Big by its entry date and source amoung members
} 2. the other to report any progress in backtracking to the origin
} that is known or reported by anyone anywhere through out the
} world as one or more of us becomes aware of it.
}
} sterling
}
}
} On Thu, 28 Aug 2003, Warren E Straszheim wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Going with Linux or Mac OS will probably ensure that you don't get infected
} } by a virus, but it won't spare you from receiving all the fool traffic that
} } these viruses have generated. These viruses are equal opportunity pains in
} } the butt in that regard.
} }
} } But I agree with you, Windows is a high-profile target for virus writers.
} } Those of us using it cannot afford to let our guard down and say "it won't
} } happen to me". We should all have safeguards in place and make sure that
} } virus definitions are up to date if we are going to connect to the net.
} }
} } Then there is also the often forgotten caution, don't open unexpected
} } attachments. I don't care how cute the clip or website is alleged to be, I
} } don't care to risk my computer over that. And if someone is sending me a
} } document, I want to have a clear indication that it is something worthwhile
} } and and authentic and safe. Messages supposedly from colleagues that say
} } simply "Here is the new file - check it out" don't do much to certify that
} } it is a file that I should trust.
} }
} } BTW, Gordon, one of the virus copies was _allegedly_ from you.
} }
} } You all be careful out there.
} } Warren
} }
} } At 12:20 PM 8/28/2003 -0700, you wrote:
} }
} } } Or better yet, go with Linux...
} } }
} } } Or if you can't live without Windows - just get rid of outlook express on
} } } every windows machine you have. Replace it with something better like
} } } Eudora that doesn't have so many security flaws.
} } }
} } } \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} } } Gordon Ante Vrdoljak Electron Microscope Lab
} } } ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} } } gvrdolja-at-nature.berkeley.edu UC Berkeley
} } } phone (510) 642-2085 Berkeley CA 94720-3330
} } } fax (510) 643-6207 cell (510) 290-6793
} } }
} } } On Thu, 28 Aug 2003, Materials Research Laboratories, Inc. wrote:
} } } }
} } } } Yet another reason to go with Macintosh! Not a problem on our end..
} } } }
} } } } Carol Jean Hirt, VP
} } } } Materials Research Laboratories, Inc.
} } } } 800-424-1776
} } } } www.mrllab.com
} }
} } -------------------------------------------
} } No files should be attached to this message
} } -------------------------------------------
} } Warren E. Straszheim, Ph.D.
} } Materials Analysis and Research Lab
} } Iowa State University
} } 46 Town Engineering
} } Ames IA, 50011-3232
} }
} } Ph: 515-294-8187
} } FAX: 515-294-4563
} }
} } E-Mail: wesaia-at-iastate.edu
} } Web: www.marl.iastate.edu
} }
} } Scanning electron microscopy, x-ray analysis, and image analysis of materials
} } Computer applications and networking
} }
} }
} }
}
}



From daemon Sat Aug 30 02:02:14 2003



From: Alan Davis :      adavis-at-saipan.com
Date: Sat, 30 Aug 2003 12:50:59 +1000
Subject: Nikon Coolpix questions

Contents Retrieved from Microscopy Listserver Archives
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As the recipient of an excellent grant, I am contemplating a purchase of
a digital camera for my High School classroom, to be used often on
microscopes of various kinds---with 23mm and 30mm id eyepiece tubes, one
of them a trinocular scope. These are not "up-to-date" scopes. Be that
as it may. I would like to request comments from list members, who in
the past have given lots of such advice. I apologize for covering
familiar territory.

I note that many microscopists have mentioned Coolpix's on this list. I
am interested in probably either a Coolpix or a Canon 1D EOS digital
camera.

Although I would appreciate comments from users of the Canon, I fear
that the 1:1.6 cmos photodetector size to 35mm film size would render it
difficult to focus the EOS with a straightforward adaptor such as the
Diagnostic Instruments adaptors for 35mm cameras---which works QUITE
well with a Canon film EOS. THis was my plan, but, again, I am pretty
discouraged by potential focusing problems.

I have ruled out the Olympus E20N, after some experience and alot of
vignetting.

The Coolpix 5000 looks interesting. It does much of what I need,
including monitoring the live specimen on a TV, Computer, or video
projector. I like some of the features of the 5700, including better
telephoto range for shore bird studies. I have a couple of questions I
would refer to those with Coolpix experience:

How well does the 5700 work on a scope?
Does the 5700 identically to the 5000 out of the box, with the Nikon
adaptor kit?
Are these cameras significantly better than the 4500?
Are macro kits available to enable focus to within 1/8"? (I intend
to use it as an "aquarium microscope" for example to study the community
at the sediment/water column interface, for which the Videoflex camera
works admirably well.)
Can one take a shot easily an within ordinary lag values while
monitoring in real time?
Nikon has a relay lens (is that a projection lens?) for this
purpose, that will work on Coolpix 5000; does it work ok on the 5700?


Another question: is the Nikon D-100 worth the extra cost, and does it
incorporate the video monitoring Coolpix features? Is it useable on a
scope, and how?

Thank you for any comments or suggestions. I apologize for asking so
many questions at once...

Alan Davis
Marianas High School
Saipan





I wish to ask whether microscopists feel it


--
adavis-at-saipan.com 1-670-322-6580
Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI

I have steadily endeavored to keep my mind free, so as to give up any
hypothesis, however much beloved -- and I cannot resist forming one
on every subject -- as soon as facts are shown to be opposed to it.
-- Charles Darwin (1809-1882)

The right to search for truth implies also a duty; one must not
conceal any part of what one has recognized to be true.
-- Albert Einstein

As we enjoy great advantages from the inventions of others we should
be glad of an opportunity to serve others by any invention of
ours, and this we should do freely and generously.
-- Benjamin Franklin





From daemon Sat Aug 30 15:31:50 2003



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Sat, 30 Aug 2003 16:19:39 -0700
Subject: Fwd: GFP antibody

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debby-
Usually, GFP is fluorescent or luminesces on it own part, and it
isn't necessary to localize it with an antibody.

Here, there seems to be some confusion about the subject protein.
GFP is not a rabbit protein. If the protein of interest is being
localized with a rabbit antibody, that makes sense. But what is the
reagent with the GFP?
Carol Heckman
(Bowling Green State University)

} Date: Fri, 29 Aug 2003 17:28:42 -0500
} Subject: GFP antibody
} From: Debby Sherman {dsherman-at-purdue.edu}
} To: "message to: MSA list" {microscopy-at-sparc5.microscopy.com}
} X-Virus-Scanned: by amavisd-new on Purdue Mailhub
} X-Sig: 11b694fff7e42b4e8f56e22d68ad0d62
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Aug 30 18:45:12 2003



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sat, 30 Aug 2003 18:39:50 -0500
Subject: test with owner of postfix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


the appended file now has an owner of postfix


From daemon Sat Aug 30 18:51:52 2003



From: zaluzec-at-microscopy.com
Date: Sat, 30 Aug 2003 18:47:56 -0500
Subject: Administrivia: Nestor goofs....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry Colleagues...

That last message was an error. I am testing some new
software in an attempt improve performance and goofed.

Nestor
Your Friendly Neighborhood SysOp


From daemon Sun Aug 31 05:14:38 2003



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Sun, 31 Aug 2003 12:17:10 -0500
Subject: Re: GFP antibody

Contents Retrieved from Microscopy Listserver Archives
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Carol
GFP is fluorescent, but there are many uses for antibodies against it
nevertheless, including immunoblotting, immunoprecipitation and
detection of the GFP-labelled protein by immunogold labelling for
electron microscopy in the absence of antibodies against the target
protein itself.

What is presumably being requested is a recommendation for a reliable
anti-GFP.

Chris

----- Original Message -----
} From: "Carol Heckman" {heckman-at-bgnet.bgsu.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Sunday, August 31, 2003 12:19 AM


Carol,

You are correct in that GFP is often inserted into the code of a
particular protein so that it is replicated as the protein in replicated.
Then the fluorescent properties of the GFP molecule can be used to localize
the particular target protein using fluorescent or confocal microscopy.
However, this fluorescence is not useful if you want to do higher resolution
localization of the protein. For that you need TEM immunocytochemical
procedures utilizing a marker such as colloidal gold.

It is very helpful to identify GFP in living tissue and then take that same
tissue, prepare it for TEM ICC and then localize the protein using an
antibody against the GFP. If that antibody is made in rabbits than you need
an anti-rabbit colloidal gold IgG probe.

What I wanted was suggestions on where to get the antibody against GFP
that was been successfully used for TEM ICC. I would like to try a second
antibody to make sure my localization, or lack of such, is not due to the
antibody we have. Often an antibody will work well in Westerns, such as the
one we have, and not work as well under ICC conditions. This is just like
how one rabbit will produce what looks like a great antibody and another
rabbit will produce one that does not appear to be as strong but actually
will work better. Don't ask me why,...it just happens.

I did lead you astray in that I said rabbit -GFP in my original post. I am
sorry for that in that It should have read rabbit antibody against GFP. We
always have lots of anti-rabbit colloidal gold tagged IgG around so prefer
rabbit antibodies. However, we can easily get IgG from other sources so
that should not be a limit if there is a GFP antibody out there from another
organism that works better than the rabbit ones.

Debby


On 8/30/03 6:19 PM, "Carol Heckman" {heckman-at-bgnet.bgsu.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Debby-
} Usually, GFP is fluorescent or luminesces on it own part, and it
} isn't necessary to localize it with an antibody.
}
} Here, there seems to be some confusion about the subject protein.
} GFP is not a rabbit protein. If the protein of interest is being
} localized with a rabbit antibody, that makes sense. But what is the
} reagent with the GFP?
} Carol Heckman
} (Bowling Green State University)
}
} } Date: Fri, 29 Aug 2003 17:28:42 -0500
} } Subject: GFP antibody
} } From: Debby Sherman {dsherman-at-purdue.edu}
} } To: "message to: MSA list" {microscopy-at-sparc5.microscopy.com}
} } X-Virus-Scanned: by amavisd-new on Purdue Mailhub
} } X-Sig: 11b694fff7e42b4e8f56e22d68ad0d62
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi,
} }
} } Can anyone recommend a source for an antibody against rabbit-GFP to use
} } for TEM immunocytochemistry? We are trying to localize a protein with a GFP
} } construct in arabidopsis root tissue. We have tried one antibody but
} } labeling is poor and not well localized. We want to make sure that it is
} } not the antibody. The protein is an unknown and the research group has not
} } yet been able to develop a clean antibody against it.
} }
} } Debby
} }
} } Debby Sherman, Manager Phone: 765-494-6666
} } Life Science Microscopy Facility FAX: 765-494-5896
} } Purdue University E-mail: dsherman-at-purdue.edu
} } S-052 Whistler Building
} } 170 S. University Street
} } West Lafayette, IN 47907
}
}
}



From daemon Mon Sep 1 16:04:18 2003



From: Eve Lorraine Donnelly :      eld26-at-cornell.edu
Date: Mon, 1 Sep 2003 16:43:07 -0400 (EDT)
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
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Listers,

Apologies for the late reply. I don't want to overdo this topic, but few
peeps have addressed one of the original questions: is there much danger
to getting abrasive sloshed in your eye?

While SiC/water splashes might not be much of a chemical irritant, aqueous
suspensions of metal oxide particles are typically not pH-neutral, e.g.,
alumina suspensions--} acidic, silica suspensions--} basic. So, if you work
with those types of polishing suspensions, an eye splash seems likely to
be more painful than just the irritation associated with having gritty
stuff in your eye.

I work in a lab where I wear safety glasses and frequently go back and
forth between the polisher and the optical microscope. It's a bit of a
pain to take the glasses off and on, but you get used to it, and the
slight inconvenience is worth preventing a splash in the eye.

Eve


--
Eve Donnelly
Experimental Biomechanics Lab
Sibley School of Mechanical & Aerospace Engineering
Cornell University
130 Upson Hall
Ithaca, NY 14853
tel. 607.255.3582
fax. 607.255.1222




From daemon Mon Sep 1 23:42:07 2003



From: shashi singh :      shashis_99-at-yahoo.com
Date: Mon, 1 Sep 2003 21:34:46 -0700 (PDT)
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Diana,

I have two suggestions:
1. Cryo sections of the same should help
2. Application of alcohol helps to loosen the
epithelium which can then be gently peeled off. This
techique is used to debride the corneal epithelium
during surgeries.
Shashi
CCMB, Hyderabad
INDIA


Hi all,

I've been asked to see what the EM difference is
between rat eye lens
epithelium in culture and in situ. So I'd like to fix
the epithelium
while it is still in the eye. I'm worried that if I do
that it then
won't be possible to get the epithelium off the lens
without damaging
it; I also seem to remember that lens becomes brittle
and difficult
to handle when fixed. I'd like to keep life simple, so
it may be
necessary to take the epithelium off the lens before
fixing. Does
anyone have experience with this?

Thanks,

Diana




=====
Shashi Singh
Scientist
Centre for Cellular and Molecular Biology
Hyderabad-500 007
INDIA
PH-91-40-7192575,7192761,7192615
FAX-91-40-7160591, 7160311

__________________________________
Do you Yahoo!?
Yahoo! SiteBuilder - Free, easy-to-use web site design software
http://sitebuilder.yahoo.com


From daemon Tue Sep 2 10:09:49 2003



From: Carolyn.Gondran-at-sematech.org
Date: Tue, 02 Sep 2003 10:01:09 -0500
Subject: TEM Analyst position opening at International Sematech in Austin,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Temporary position (contractor status) offering 40 hrs/week with limited benefits

Job Description:
Transmission Electron Microscopist / Analyst in Process Characterization Lab supporting the Advanced Technology Development Facility (ATDF) as well as both internal and external projects at International SEMATECH (ISMT) in Austin, Texas. The TEM group at ISMT provides materials characterization in support of research and process development projects for future integrated circuit manufacturing. Our research projects tend to focus on materials issues several years ahead of current semiconductor industry manufacturing trends, we therefore often study materials systems that offer new and exciting characterization problems and often collaborate with leading researchers at national labs and universities around the world, however we also have a significant workload involving routine characterization and process monitoring for systems that are already well understood.

Required:
M.S. or Ph.D. in Materials Science, Solid-State Physics or Chemistry with strong analytical TEM experience.

For more details please contact:
Brendan Foran -at- Brendan_Foran-at-SEMATECH.org

International SEMATECH, headquartered in Austin, Texas, is a global consortium of leading semiconductor manufacturers that represent about half the world's semiconductor production. International SEMATECH engages in cooperative, precompetitive efforts to improve semiconductor manufacturing technology through the support of our members. To learn more about International SEMATECH, visit our website at www.sematech.org.


From daemon Tue Sep 2 11:33:33 2003



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 02 Sep 2003 12:26:12 -0400
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} not related to the real safety (mostly keeping paperwork in a good shape
} and accurately fill out chemical waste disposal tags). So, in such
} situation, I think, it's superviser's personal responsibility to provide
} safely environment to the workers and train them accordingly.

Look, we don't want to have this devolve into a session on what is safe and
what isn't, but this is a very simplistic view. Paperwork may be essential
for real safety of all handlers of material down the chain of disposal. If
a supervisor has a limited budget, he/she is going to scrimp on safety.

Policies are in place to protect workers who historically have been
mistreated. Yes, blanket policies do not apply in all situations. The
bureaucracy simply needs some flexibility for individually documented
exemptions.

} P.S. By the way: in my EM work, my glasses, I wear to correct my vision,
} save my eyes in uncounted number of times. I, also, used to remove them
} every time I am using light microscope or binoculars. I don't see big
} problem removing them if necessary. But: yes, it decreases my
} productivity. Ok, let say, I need about 5 sec. to remove them and put
} back. Let say I do it 40 times a day: 5 secx40=200 sec/3.3 min per day.

But you're wearing gloves and your gloves shouldn't be near your unshielded
eyes each time you have to handle the googles. So you really should take
off your gloves before removing your goggles each time.



____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/




From daemon Tue Sep 2 12:32:23 2003



From: Richard Olsson :      richard-at-polymer.kth.se
Date: Tue, 2 Sep 2003 18:58:16 +0200
Subject: TEM-alt. to counted size distribution

Contents Retrieved from Microscopy Listserver Archives
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Dear Listeners,
I am making ferrite nanoparticles doped with Co and/or Mn in the size
range of 20-100nm. I have looked at them with TEM and made up size
distributions from the pictures. However, I would now like to check my
distribution curves with a particle sizer. Does anyone now if this is
possible? I suspect it to be difficult since my particles are magnetic.
If it is possible, does anyone know what type of medium I can disperse
my particles in?
So far I have tried to alter the pH but it doesn't seem to be enough
and the particles still agglomerate before the the measurement is over.
Thanks .

Yours sincerely
Richard

PhD student, Swedish Defense Research Agency
Department of Polymer Technology
Royal Institute of Technology
Teknikringen 56
SE-100 44 Stockholm
SWEDEN
phone: +46-8-790 76 37

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solely for the person(s) to whom they are addressed and contain
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recipients is prohibited and may be illegal. If you are not an intended
recipient, please immediately inform the sender and send him/her back
the present e-mail and its attachments and destroy any copies which may
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From daemon Tue Sep 2 13:18:22 2003



From: Jim Haley :      haley-at-mvia.com
Date: Tue, 02 Sep 2003 14:13:32 -0400
Subject: Re: Nikon Coolpix questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Alan,

Our webppage on connecting a Coolpix to a microscope will answer a lot of your
questions:
http://www.mvia.com/Coolpix/clpxadpt.htm

Also, please see my answers below...

Thanks!
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
125 Sherwood Drive
Monaca, PA 15061
voice: (724) 728-7493
fax: (412) 291-1709
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************


Alan Davis wrote:
}
} The Coolpix 5000 looks interesting. It does much of what I need,
} including monitoring the live specimen on a TV, Computer, or video
} projector. I like some of the features of the 5700, including better
} telephoto range for shore bird studies. I have a couple of questions I
} would refer to those with Coolpix experience:
}
} How well does the 5700 work on a scope?

Very poorly - you'll need to use the digital zoom mode on the 5700. See our FAQ
section: http://www.mvia.com/Coolpix/clpxadpt.htm#FAQ

} Does the 5700 identically to the 5000 out of the box, with the Nikon
} adaptor kit?

NO! You'll need to use the digital zoom mode on the 5700 as opposed to the
optical zoom mode on the 5000, which will result in very poor images.

} Are these cameras significantly better than the 4500?

The 5000 has a higher resolution, but does not have a swivel body as the 4500
model does, which make it a little less ergonomical. However, the 500 will give
you better images.

} Are macro kits available to enable focus to within 1/8"? (I intend
} to use it as an "aquarium microscope" for example to study the community
} at the sediment/water column interface, for which the Videoflex camera
} works admirably well.)

There are a couple of lenses available from Nikon for various wide angle and
fisheye applications.

} Can one take a shot easily an within ordinary lag values while
} monitoring in real time?

Yes, but the live image will pause when you capture your image.

} Nikon has a relay lens (is that a projection lens?) for this
} purpose, that will work on Coolpix 5000; does it work ok on the 5700?
}
} Another question: is the Nikon D-100 worth the extra cost, and does it
} incorporate the video monitoring Coolpix features? Is it useable on a
} scope, and how?
}
} Thank you for any comments or suggestions. I apologize for asking so
} many questions at once...
}
} Alan Davis
} Marianas High School
} Saipan
}
} I wish to ask whether microscopists feel it
}
} --
} adavis-at-saipan.com 1-670-322-6580
} Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI
}
} I have steadily endeavored to keep my mind free, so as to give up any
} hypothesis, however much beloved -- and I cannot resist forming one
} on every subject -- as soon as facts are shown to be opposed to it.
} -- Charles Darwin (1809-1882)
}
} The right to search for truth implies also a duty; one must not
} conceal any part of what one has recognized to be true.
} -- Albert Einstein
}
} As we enjoy great advantages from the inventions of others we should
} be glad of an opportunity to serve others by any invention of
} ours, and this we should do freely and generously.
} -- Benjamin Franklin
}
}

--


From daemon Tue Sep 2 15:40:44 2003



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Tue, 2 Sep 2003 16:34:06 -0400
Subject: Re: Torr Seal Questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is our answer to the many responses concerning the previous thread on
Torr Seal.
Torr Seal is an extremely high quality epoxy resin manufactured under a
private label agreement as Torr Seal.
Since there are non-critical applications which do not require such a high
quality epoxy we, like others do carry a product known as a Torr Seal
equivalent.
We do let all our users know that when they select an 'equivalent they are
paying less, but naturally there is a sacrifice in quality.

John Arnott

Disclaimer: Ladd Research sells the products mentioned in this e-mail.

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: sales-at-laddresearch.com




From daemon Tue Sep 2 22:42:29 2003



From: Richard Olsson :      richard-at-polymer.kth.se (by way of
Date: Tue, 2 Sep 2003 22:28:57 -0500
Subject: Nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listeners,
I am making ferrite nanoparticles doped with Co and/or Mn in the size range of 20-100nm. I have looked at them with TEM and made up size distributions from the pictures. However, I would now like to check my distribution curves with a particle sizer. Does anyone now if this is possible? I suspect it to be difficult since my particles are magnetic. If it is possible, does anyone know what type of medium I can disperse my particles in?
So far I have tried to alter the pH but it doesn't seem to be enough and the particles still agglomerate before the the measurement is over. Thanks .

Yours sincerely
Richard

PhD student, Swedish Defense Research Agency
Department of Polymer Technology
Royal Institute of Technology
Teknikringen 56
SE-100 44 Stockholm
SWEDEN
phone: +46-8-790 76 37

---------------------------------------------------------
Legal Notice: This electronic mail and its attachments are intended solely for the person(s) to whom they are addressed and contain information which is confidential or otherwise protected from disclosure, except for the purpose they are intended to. Dissemination, distribution, or reproduction by anyone other than their intended recipients is prohibited and may be illegal. If you are not an intended recipient, please immediately inform the sender and send him/her back the present e-mail and its attachments and destroy any copies which may be in your possession.

---------------------------------------------------------


From daemon Tue Sep 2 22:47:34 2003



From: Jlindstrom-at-hotmail.com (by way of Ask-A-Microscopist)
Date: Tue, 2 Sep 2003 22:42:32 -0500
Subject: Ask-A-Microscopist: well slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Jlindstrom-at-Hotmail.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, September 2, 2003 at 17:19:35
---------------------------------------------------------------------------

Email: Jlindstrom-at-Hotmail.com
Name: james lindstrom

Organization: Timberline High School

Education: 9-12th Grade High School

Location: Boise, ID, USA

Question: When would you use a well slide?

---------------------------------------------------------------------------


From daemon Tue Sep 2 22:49:07 2003



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 02 Sep 2003 20:47:18 -0700
Subject: Feedback on JEOL 6700F

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate off-list feedback from
users of JEOL 6700F tools.

I'm looking at a system with turbo pump,
large specimen exchange interlock, solid
state BSE vs. Robinson BSE, beam stability,
accommodation to EDAX Genesis EDS and overall
usability and support. Location is Northern CA.

Off-list please. All are confidential.
Trying to make a purchase decision between
this and comparable Hitachi. Specimens are
microchips in cross section, top down, metallurgical
coupons of IC manufacturing tools, legacy support
for Amray 12mm diameter 3.1mm pin specimen stubs.
Also, there is a need for working with rather large
specimens. Like blocks of NIST standards (100mm
diameter, 50mm high).

Native digital capture resolution is low. Are
there other options that work? Is anyone using
Soft Imaging ADDA? 4Kx4K pixels minimum is needed.

tnx,
gary g.



From daemon Wed Sep 3 03:27:18 2003



From: Gordon Couger :      gcouger-at-couger.com
Date: Wed, 3 Sep 2003 03:20:18 -0500
Subject: Re: Safety Glasses while polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} From: "Michael Cammer" {cammer-at-aecom.yu.edu}
:
: } not related to the real safety (mostly keeping paperwork in a
good shape
: } and accurately fill out chemical waste disposal tags). So, in
such
: } situation, I think, it's superviser's personal responsibility to
provide
: } safely environment to the workers and train them accordingly.
:
: Look, we don't want to have this devolve into a session on what is
safe and
: what isn't, but this is a very simplistic view. Paperwork may be
essential
: for real safety of all handlers of material down the chain of
disposal. If
: a supervisor has a limited budget, he/she is going to scrimp on
safety.
:
: Policies are in place to protect workers who historically have
been
: mistreated. Yes, blanket policies do not apply in all situations.
The
: bureaucracy simply needs some flexibility for individually
documented
: exemptions.
:
: } P.S. By the way: in my EM work, my glasses, I wear to correct my
vision,
: } save my eyes in uncounted number of times. I, also, used to
remove them
: } every time I am using light microscope or binoculars. I don't see
big
: } problem removing them if necessary. But: yes, it decreases my
: } productivity. Ok, let say, I need about 5 sec. to remove them
and put
: } back. Let say I do it 40 times a day: 5 secx40=200 sec/3.3 min
per day.
:
: But you're wearing gloves and your gloves shouldn't be near your
unshielded
: eyes each time you have to handle the googles. So you really
should take
: off your gloves before removing your goggles each time.
:
Face shields will solve the glove problem. If the curve of plastic
face shields interferes with your work a welders helmet with plain
glass or you prescription lenses could be used. You would need some
clearance behind the microscope but it would speed things up and not
run the risk of getting something in your eye when you were removing
goggles. With prescription lens in the helmet it should speed up
work as you don't have to remove your glasses. It also solves the
problem of scratched face shields.

Here is an example
http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=2551210520&category=633
The part below the lens should be cut away as you don't need
protection from the burning rays of the welders arc and the dark
lens replaced with a clear one. Then just the push of your thumb
will stand the helmet straight up out of the way of a microscope.

A welders helmet should be sturdy enough to satisfy any safety
officer and quicker to tip up than removing goggles. Some surgery on
the hood would make it lighter and reduce the clearance need behind
the microscope with out compromising eye safety.

There are also some goggles that have a flip up lens available at
welders supply houses as well. I don't know if they would clear a
microscope. Here is an example
http://cgi.ebay.com/ebaymotors/ws/eBayISAPI.dll?ViewItem&category=35000&item=2429373537
There are other designs that might be more suitable with the lens
swinging 180 degrees. A call to your local Air Gas or other welding
supply dealer will let you know what is available.

Gordon Couger gcouger-at-couger.com

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward any links or information you think might be useful to
others.




From daemon Wed Sep 3 03:53:42 2003



From: =?ISO-8859-2?Q?Old=F8ich_Benada?= :      benada-at-biomed.cas.cz
Date: Wed, 03 Sep 2003 10:51:53 +0200
Subject: ZEM 1000CW trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,
We have following trouble with Zephyr ZEM 1000CW watercooling unit:
When our CM12 is switched on from STAND BY, overload protector switches off the
pump motor of ZEM 1000CW from time to time. If the reset button of overload
protector is pressed down, the motor starts and all is OK. But after several
days or weeks this occurs again. Please, can you give us some hints or
suggestions.
Many thanks in advance. Oldrich Benada

+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-241062399
Fax: +420-241062347
WEB: http://www.biomed.cas.cz/mbu/lem113/lem.htm




From daemon Wed Sep 3 03:53:50 2003



From: dobrusia budka :      dobrusiabudka-at-tlabs.ac.za
Date: Wed, 03 Sep 2003 10:40:42 +0200
Subject: manual for Reichert FC4 cryoultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I am searching for a manual for Reichert FC4 cryoultramicrotome.
Is there anybody who could help me with that?
Best regards,

Dr Dobroslawa Budka
Materials Research Group
iThemba LABS
PO Box 722
Somerset West 7129
South Africa
fax: +27-21-8433543
phone: +27-21-8431161
mobile phone: 0722656091





From daemon Wed Sep 3 06:43:34 2003



From: ldm :      ldm2-at-risc4.numis.nwu.edu
Date: Wed, 3 Sep 2003 06:38:02 -0500 (CDT)
Subject: TEM Software Beta Testers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for volunteers to perform beta testing of
some code to do HREM image processing and Direct Methods.
The code is a gnu-like package (i.e. free) which should compile
on any unix or Mac computer fairly simply using a conventional
"./configure ; make ; make install" strategy. If you know
what I am talking about and are interested, please email
me (not the whole list). Thanks.

-----------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm2-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
-----------------------------------------------




From daemon Wed Sep 3 07:45:25 2003



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Wed, 03 Sep 2003 09:40:35 -0300
Subject: help with J. Royal Microsc. Society reprint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I just about wrote "Urgent Assistance Needed" as the subject line,
but then realized that would probably not make it past several dozen spam
filters. I'm in my usual position (stuck between a rock and a deadline).
I'm looking
for a copy (original reprint or the journal issue) of:

MANTON I. & VON STOSCH H.A. 1966. Observations on the fine structure
of the male gamete of the marine centric diatom Lithodesmium undulatum.
Journal of
the Royal Microscopical Society 85: 119-134.

I need to scan several figures from the paper, and our interlibrary
loans can only
get a xerox copy. Can anybody help? I'd gladly pay courier charges (both
ways
if you need the journal/paper back). Alternatively, if someone is
willing, they could
scan the figures from their end, but it would most likely be all of the
plates in the paper,
as I'm not sure right now which ones I need.

If you help, I'll make several offerings on your behalf to the gods of
microscopy, requesting
long filament life, long strings of buttery smooth silver-gray thin
sections, and awesome
powers against the Dark Forces of Bureaucracy. I'll also put you on the
list to receive
the coveted large format scan of the Philips 200 column cross-section
poster (discussed
earlier in this forum) which I should have constructed and cleaned up
Real Soon Now.

Please contact me off-list. Thanks,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Wed Sep 3 09:19:00 2003



From: Francisco Javier Hernandez Blazquez :      fjhblazq-at-usp.br
Date: Wed, 3 Sep 2003 11:13:09 -0300
Subject: LM-immunohistochemistry:paraffin slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues

I'm trying to stain liver sections embedded in paraffin and fixed by metacarn
fixative. My first antibody is monoclonal. The problem is that I only have a
positive signal at the periphery of the section and nothing in the middle.
Does someone have any clue why this could happen?
Thanks in advance.

Prof. Dr. Francisco Javier Hernandez Blazquez
Universidade de São Paulo
Fac. de Medicina Veterinária e Zootecnia
Departamento de Cirurgia - Setor de Anatomia
Av. Prof. Dr. Orlando Marques de Paiva, 87
05508-000 - São Paulo (SP)
Tel..55 (11) 3091 1374
Fax 55 (11) 3091 7805
email: fjhblazq-at-usp.br



From daemon Wed Sep 3 12:22:57 2003



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Wed, 03 Sep 2003 14:15:10 -0300
Subject: Re: help with J. Royal Microsc. Society reprint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the quick reply, Chris (and others who may be in the pipe),

Even my good luck seems to have a bad side! Not 5 minutes after posting
this request,
I found a copy of this paper, literally under my nose. Embarassing to
say the least!
I'll be quiet for at least a week....

Cheers,

Jim

Christopher F. Blanford wrote:

} James -
}
} Unfortunately we can't take any copies of the journal out of the
} library. But as the RMS is just up the street from me, do you think
} you could just give them a call? Either see if they have a copy they
} could send you, or if you're still in a bind, see if they'd loan me a
} copy for a day and I could scan in the pages you want. The number: +44
} 1865 248 768.
}
} Good luck.
}
} Chris
}
} On Wednesday, September 3, 2003, at 01:40 pm, James M. Ehrman wrote:
}
} } Hi all,
} }
} } I just about wrote "Urgent Assistance Needed" as the subject line,
} } but then realized that would probably not make it past several dozen
} } spam
} } filters. I'm in my usual position (stuck between a rock and a
} } deadline). I'm looking
} } for a copy (original reprint or the journal issue) of:
} }
} } MANTON I. & VON STOSCH H.A. 1966. Observations on the fine structure
} } of the male gamete of the marine centric diatom Lithodesmium
} } undulatum. Journal of
} } the Royal Microscopical Society 85: 119-134.
} }
} } I need to scan several figures from the paper, and our interlibrary
} } loans can only
} } get a xerox copy. Can anybody help? I'd gladly pay courier charges
} } (both ways
} } if you need the journal/paper back). Alternatively, if someone is
} } willing, they could
} } scan the figures from their end, but it would most likely be all of
} } the plates in the paper,
} } as I'm not sure right now which ones I need.
} }
} } If you help, I'll make several offerings on your behalf to the gods
} } of microscopy, requesting
} } long filament life, long strings of buttery smooth silver-gray thin
} } sections, and awesome
} } powers against the Dark Forces of Bureaucracy. I'll also put you on
} } the list to receive
} } the coveted large format scan of the Philips 200 column cross-section
} } poster (discussed
} } earlier in this forum) which I should have constructed and cleaned up
} } Real Soon Now.
} }
} } Please contact me off-list. Thanks,
} }
} } Jim
} }
} } --
} }
} } James M. Ehrman
} } Digital Microscopy Facility
} } Mount Allison University
} } Sackville, NB E4L 1G7
} } CANADA
} }
} } phone: 506-364-2519
} } fax: 506-364-2505
} } email: jehrman-at-mta.ca
} } www: http://www.mta.ca/~jehrman
} }
} }
} }
} }
} }

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Wed Sep 3 14:28:16 2003



From: taylor-at-bio.fsu.edu (by way of MicroscopyListserver)
Date: Wed, 3 Sep 2003 14:20:35 -0500
Subject: Submit MicroscopyListserver via WWW Form: protocol for fixing,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (taylor-at-bio.fsu.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 3, 2003 at 11:21:33
---------------------------------------------------------------------------

Email: taylor-at-bio.fsu.edu
Name: Kenneth Taylor

Organization: Florida State University

Title-Subject: Fixation and Embedding of bacteria

Question: Hi,

I am looking for a protocol for fixing, embedding and sectioning bacteria, in particular E. coli. Good membrane preservation would be important for this study.

Does anyone have a protocol that they would be willing to share?

Cheers -- Ken


---------------------------------------------------------------------------


From daemon Wed Sep 3 21:03:56 2003



From: Jonathan McGovern :      semrus-at-shaw.ca
Date: Wed, 03 Sep 2003 19:56:10 -0600
Subject: SEM - Used sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All;

Still looking for a used sputter coater with a carbon fiber evaporation
attachment.
Please contact me off line if you have a lead on one.

Cheers;

Jon McGovern
semrus-at-shaw.ca




From daemon Wed Sep 3 22:52:12 2003



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 03 Sep 2003 20:44:00 -0700
Subject: JEOL 6700F, HI S4800, S5200

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK...thanks to all of the respondents about
FESEMs. I did receive some good feedback.

I am trying to make a budget wedge for FY04 and
need to know a basic idea of benefits of what I
have selected as candidate systems. That said,
I also need to put in funding wedges to cover
the venue of appropriate SEMs.

JEOL 6700F
Hitachi S-4800
Hitachi S-5200

Other options are welcome too.

I would appreciate knowing personal experiences
based on differing sizes of specimens and different
types of analysis. Budget time comes along one time,
and is perversely ignorant of systems. Caveat emptor.

It may be time to upgrade. But to what? Cold
FE with accommodation of my EDAX Cryospec is necessary.
Robinson BSE is also a big plus.

Ideas? Experiences?

Off-list, of course.

gary g.



From daemon Thu Sep 4 06:43:17 2003



From: dobrusia budka :      dobrusiabudka-at-tlabs.ac.za
Date: Thu, 04 Sep 2003 13:26:10 +0200
Subject: historesin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
could you please let me know who the suppliers of HISTORESIN are. It is a
glycol methacrylate usful for light microscopy. It used to be marketed
originally as LKB HISTORESIN.
Thank you,
Dobrusia


Dr Dobroslawa Budka
Materials Research Group
iThemba LABS
PO Box 722
Somerset West 7129
South Africa
fax: +27-21-8433543
phone: +27-21-8431161
mobile phone: 0722656091





From daemon Thu Sep 4 08:08:08 2003



From: reckert-at-nwlabs1896.com (by way of MicroscopyListserver)
Date: Thu, 4 Sep 2003 08:02:38 -0500
Subject: Submit MicroscopyListserver via WWW Form: CamScan CS44 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (reckert-at-nwlabs1896.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 3, 2003 at 21:24:36
---------------------------------------------------------------------------

Email: reckert-at-nwlabs1896.com
Name: Rainer Eckert

Organization: ASM

Title-Subject: MListserver:

Question: We are interested in purchasing a CamScan CS44 SEM (appr. 10 years old) and I was wondering if anybody could share their experience (weak and strong points) about the scope. Thank you.

---------------------------------------------------------------------------


From daemon Thu Sep 4 12:15:14 2003



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 04 Sep 2003 12:10:19 -0500
Subject: Anyone know PRIVAT or MINIHOTEL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well, BILLPC has stopped sending me copies of a virus, but I still get
copies from
PRIVAT (pD9E76707.dip.t-dialin.net [217.231.103.7], and
MINIHOTEL (modemcable027.214-131-66.nowhere.mc.videotron.ca [66.131.214.27]

Some of the spoofed addresses/domains on the messages are ones I recognize
from this list. So if any of you recognize these folks or their domains,
would you please tell them to check out their machines. Thanks.

Warren




From daemon Thu Sep 4 13:41:35 2003



From: Omayra Velez :      mayas003-at-yahoo.com
Date: Thu, 4 Sep 2003 14:04:50 -0700 (PDT)
Subject: Nerve tissue problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello
Historesin is now produced and sold by Leica Microsystems Nussloch GmbH.
Their address is Heidelberg Strabe 17-19
D-69226 Nussloch/Heidelberg Tel. 0 62 24/143-0
You may look at their homepage at
http://www.histo-solutions.com/website/sc_hbu.nsf

Good luck

Prof. Dr. Francisco Javier Hernandez Blazquez
University of São Paulo
Fac. Veterinary Medicine and Animal Sciences
Departament of Surgery - Sector of Anatomy
Av. Prof. Dr. Orlando Marques de Paiva, 87
05508-000 - São Paulo (SP) - Brasil
Tel..55 (11) 3091 1374 Fax 55 (11) 3091 7805
email: fjhblazq-at-usp.br

----- Original Message -----
} From: "dobrusia budka" {dobrusiabudka-at-tlabs.ac.za}
To: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, September 04, 2003 8:26 AM


Hi:

Help!!! I'm presently having problems with photography
of nerve tissue. My problem is that the tissue
appears shredded, and it has multiple holes and splits
along the tissue but not the plastic. Making the
tissue impossible to photo. This problem can only be
seen when we are to photograph in the microscope. 1
micron light slides look fine. The only thing we have
change is the type of fixative. We went from
glutaraldehyde to para formaldehyde, but nothing have
change from my protocol. We think is an infiltration
problem, because the tissue is big to start and
humidity have been a problem in the past in other
types of tissue, and because the holes and splits are
only in the tissue. We are looking for processing
protocols for Sural Nerve tissue in order to compare
with the one we have. If anyone can help it will be
greatly appreciated.

Thanks

Omayra Velez MS
Electron Microscopy Specialist
Pathology Department
Weill Cornell Medical College
1300 York Ave
NY,NY, 10021
212-746-6437
omv2001-at-med.cornell.edu

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From daemon Thu Sep 4 18:19:59 2003



From: Franklin Bailey :      jfb-at-uidaho.edu
Date: Thu, 04 Sep 2003 16:15:06 -0700
Subject: ISI WB-6 on ebay

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know anything about the ISI WB-6 that is currently being
offered on eBay? A friend of mine was wondering about the specifics
concerning the condition and usability of the instrument.



From daemon Thu Sep 4 20:21:02 2003



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 04 Sep 2003 18:16:00 -0700
Subject: Re: Nerve tissue problems

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Omayra
It looks like fixation/impregnation problem. Paraformaldehyde itself is
not enough for EM. I know nothing about your particular sample, in general,
we are using 4% paraformaldehyde+ 1% GA overnight (+4oC). Nervous tissue
also contains a lot of lipids, so dehydratation should be slow: actually,
all steps should be longer than usual. I do find that Spurr resin is good
for brain tissue: it penetrates better than Epon or Araldite. Good
luck, Sergey.

At 02:04 PM 9/4/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Sep 5 04:19:18 2003



From: Terry Cooper :      terry.cooper-at-btinternet.com
Date: Fri, 5 Sep 2003 10:06:57 +0100
Subject: Historesin

Contents Retrieved from Microscopy Listserver Archives
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Dear Dobrusia,

Historesin as such is now discontinued but lives on as a series of resins
produced by Heraeus Kulzer in Germany - Technovit 7100 (Historesin),
Technovit 8100 (Historesin Plus) and Technovit 9100 NEW (Histodur).

These resins are all identical to the Historesin products. TAAB Laboratories
Equipment Ltd is the Distributor for these products in the UK, but if anyone
has a problem finding the distributor in their country, I may be able to
help,

Best regards

Terry Cooper
TAAB Laboratories Equipment Ltd
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England
Tel ++44 (0)118 981 7775 Fax ++44 (0)118 981 7881
e-mail sales-at-taab.co.uk
www.taab.co.uk



From daemon Fri Sep 5 04:50:55 2003



From: Dirk Kirch :      kirch-at-imm.rwth-aachen.de
Date: Fri, 05 Sep 2003 11:50:20 +0200
Subject: EBSD-Phospor Screen problems

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Hello Everyone!


I have problems with my EBSD measurements.
The image of the phosphorscreen is distorted
at the bottom of the screen while substracting
the backgroung. instead of pure cirlces at the bottom
you see more or less straight lines cutting the circles
or the circles themselfs are distorted. When
substracting at very low magnifiocations this problem
doesnŽt occure. When you look at the RAW picture the
area at the bottom shows a dark line. Please I need help.
It is urgent


Regards Dirk Kirch
--
begin:vcard
n:Kirch;Dirk
tel;fax:+49(0)241-8022301
tel;work:+49(0)241-8026861
x-mozilla-html:FALSE
url:www.imm.rwth-aachen.de
org:Institute of Physical Metallurgy and Metal Physics;University of Aachen
version:2.1
email;internet:kirch-at-imm.rwth-aachen.de
title:Dipl.- Phys. Dirk Kirch
adr;quoted-printable:;;Kopernikusstra=DFe 14=0D=0A;52056 Aachen;Germany;;
end:vcard




From daemon Fri Sep 5 06:28:58 2003



From: Dirk Kirch :      kirch-at-imm.rwth-aachen.de
Date: Fri, 05 Sep 2003 12:50:30 +0200
Subject: EBSD-Phospor Screen problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everyone!


I have problems with my EBSD measurements.
The image of the phosphorscreen is distorted
at the bottom of the screen while substracting
the backgroung. instead of pure cirlces at the bottom
you see more or less straight lines cutting the circles
or the circles themselfs are distorted. When
substracting at very low magnifiocations this problem
doesnŽt occure. When you look at the RAW picture the
area at the bottom shows a dark line. Please I need help.
It is urgent


Regards Dirk Kirch

--
begin:vcard
n:Kirch;Dirk
tel;fax:+49(0)241-8022301
tel;work:+49(0)241-8026861
x-mozilla-html:FALSE
url:www.imm.rwth-aachen.de
org:Institute of Physical Metallurgy and Metal Physics;University of Aachen
version:2.1
email;internet:kirch-at-imm.rwth-aachen.de
title:Dipl.- Phys. Dirk Kirch
adr;quoted-printable:;;Kopernikusstra=DFe 14=0D=0A;52056 Aachen;Germany;;
end:vcard


--
begin:vcard
n:Kirch;Dirk
tel;fax:+49(0)241-8022301
tel;work:+49(0)241-8026861
x-mozilla-html:FALSE
url:www.imm.rwth-aachen.de
org:Institute of Physical Metallurgy and Metal Physics;University of Aachen
version:2.1
email;internet:kirch-at-imm.rwth-aachen.de
title:Dipl.- Phys. Dirk Kirch
adr;quoted-printable:;;Kopernikusstra=DFe 14=0D=0A;52056 Aachen;Germany;;
end:vcard




From daemon Fri Sep 5 10:21:51 2003



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Fri, 05 Sep 2003 11:18:18 -0400
Subject: liquid helium cooled double tilt TEM holder

Contents Retrieved from Microscopy Listserver Archives
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We are thinking to buy a liquid helium cooled double tilt TEM holder. Has
anyone used this type of holder? Can you please tell me your experience
with it?
I would appreciate very much if you could tell me how well it works, and
what problems it might have.

Thanks
Regards
Yan Xin



From daemon Fri Sep 5 10:44:51 2003



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Fri, 05 Sep 2003 11:44:22 -0400
Subject: US made liquid helium cooling TEM holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to ask more specifically about the liquid helium cooling TEM
holder made by USA side of the Gatan company. The UK made holder will be
too expensive for us, so anyone has experience with the US made holder?

Regards
Yan Xin



From daemon Fri Sep 5 11:59:57 2003



From: Omayra Velez :      mayas003-at-yahoo.com
Date: Fri, 5 Sep 2003 09:55:10 -0700 (PDT)
Subject: Re: Nerve problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi to all:
Thanks for the responce on the nerve problem. Some of
you have asked how big the tissue samples are and the
type of resin I'm using. We are using, EMBED 812 with
DMP-30 as catalyst. The tissue size is about 5mm x
2mm x 3mm. Big for the most part. I already tryed to
make the blocks smaller, but the nero-pathologist
wanted the pieces this big. We have a 20 hour
protocol from buffer to 100% epon. I'll try some of
the sudjestions, lenthtening the protocol. Although
we think we are also having a humidity issue as well,
due to construction and AC problems.

Thanks again

Omayra Velez
Electron Microscopy Specialist
Pathology Department
Weill Cornell Medical College
1300 York Ave
NY,NY. 10021
212-746-6437
omv2001-at-med.cornell.edu

__________________________________
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From daemon Fri Sep 5 12:15:42 2003



From: MicroscopyToday :      microtod-at-optonline.net
Date: Fri, 05 Sep 2003 13:12:23 -0400
Subject: Microscopy Today Sept/Oct Table of Contents

Contents Retrieved from Microscopy Listserver Archives
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Listers,

Here is the table of contents for the September/October 2003 issue of
Microscopy Today.

New Subscriptions via http://www.microscopy-today.com only, please
New subscriptions will close on Tuesday 9 September for this issue.

There has been a major change in subscription policies coming out of the MSA
Winter Council meeting.
Briefly: Canadians and Mexicans are now offered free subscriptions along
with microscopists in the USA. MSA members anywhere have free subscriptions.
Non-MSA, non-North American subscriptions have been reduced from $80 or
$110US to $35US. Additional details in the magazine or on our web site.

Table of Contents:

Carmichael Print Your Own Organs!
Kelly and Gribb Atom Probes LEAP Ahead
Breger Microscopy At The Ends Of The Earth
Barkan, et al. A Si Multi-Cathode Detector For Microanalysis
Applications
Sehgal, Karim, Stafford, & Fasolka Techniques for Combinatorial and
High- Throughput Microscopy
Part 1: Gradient Spec.
Fabrication for Polymer Thin
Film Research
Millette, Boltin, Few, & Turner Microscopical Studies of World Trade
Center Disaster Dust Particles
Vrdoljak Prep. Of Soil Samples For Light And TEM
Radzikowska A New Look At Cast Iron Microstructure
Lifshin & Gauvin Precision and Detection Limits for EDS Analysis in
the SEM
Sedgewick Adding Color To Grayscale Images
Downing Support Films with Uniform Hole Size
Mills Cleaning a Cold Cathode Gauge Tube
Yang and Kalab Zigzag Edges in SEM Micrographs
Schooley Microscopy CD-ROMs For Children: A Bibliography

Ron Anderson, Editor
Microscopy Today



From daemon Fri Sep 5 14:43:09 2003



From: thurston e herricks :      thurst0n-at-u.washington.edu
Date: Fri, 5 Sep 2003 12:37:04 -0700 (PDT)
Subject: Package for measuring thickness contours

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

Does anyone have a package or know of a package that can calculate changes
in sample thickness from thickness fringes observed in conventional TEM?
I am imageing defect free single crystals on the order of 100nm. I need
to know how the crystals thickness profile changes.


Take care

Thurston Herricks



From daemon Fri Sep 5 17:20:10 2003



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 05 Sep 2003 17:14:17 -0500
Subject: SEM of Magnetic particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have a student who wants to image magnetic particles with SEM. The
particles are in the 400nm to 1µm size range. I had planned to stick them
to double stick copper tape prior to imaging. However, I am concerned about
their adversely affecting the microscope. Are there any suggestions as to
mounting the particles, whether short working distance may magnify potential
problems if some particles break loose and get into the lens. Etc?

If there is a concern then I guess it would be possible to mount on
sticky carbon tape, overlay the sample with a formvar film, and then use
backscattering to image the particles. However, is this necessary?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907



From daemon Fri Sep 5 18:48:36 2003



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 05 Sep 2003 16:45:03 -0700
Subject: Beam current for EDS -- hot/cold gun

Contents Retrieved from Microscopy Listserver Archives
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Greetings to EDS experts:

If one wants to do pure standards-based
quantitative EDS analysis, protocol says
that conditions for the standards must
be the same as for the specimen. Well,
if one is using a thermal FE SEM, then
the beam current is very likely to be
the same at any time. How about for a
cold FE gun?

From what I can surmise, the cold guns
vary much more than thermal FE guns, to
the point that intervention is necessary.
Does this mean that every quant EDS
measurement has to have a precursor
Faraday cup reading of beam current?
Conversely, does this mean that this is
not required when using a thermal FE gun?

The EDAX system supports a Beam Current
Factor so that calculations are made based
on as-read current when making the quant
analysis. This seems like a lot of hassle.
Is the difference between standardless and
standards-based EDS so huge that the hassle
is warranted? Or, is this nit picking?

gary g.



From daemon Fri Sep 5 21:31:16 2003



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 05 Sep 2003 19:27:45 -0700
Subject: Re: SEM of Magnetic particles

Contents Retrieved from Microscopy Listserver Archives
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Is it possible to simply degauss the samples
before putting them in the SEM?

There is a border, I believe, between too
much degaussing and too little. Looking at
hard drive tracks is a case in point. Depending
on your ultimate resolution, the working distance
may facilitate your specimens by keeping them
away from the final lens. 1um specimens should
not require short WD.

Your BSE ought to be a separate issue depending
on what type it is and what KV it works at.

gary g.



At 03:14 PM 9/5/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Sep 6 10:11:45 2003



From: Ken Gaugler :      ken-at-gaugler.com
Date: Sat, 06 Sep 2003 07:58:31 -0700
Subject: Re: SEM of Magnetic particles

Contents Retrieved from Microscopy Listserver Archives
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Debby,

We have routinely examined (gamma-)ferric oxide particles in the SEM by
dusting the particles onto double-sided sticky carbon tape. Or you can
paint a small dab of carbon paint onto your stub and dust the particles
onto the paint while it is still wet. Then tap the side of the stub
firmly on a hard surface to dislodge any loose particles. If you are
really paranoid you can use a low-pressure gas stream (like a "Micro
Duster") to make sure there are no loose particles present. However, the
more aggressively you remove looser particles, the less you are likely
to see larger particles that may exist in the specimen. This may be
important if you are trying to do particle size distribution measurements.

Particles like Fe2O3 are weakly magnetic, so there is not much danger of
them being sucked up into your lenses by the magnetic field.

You will probably want to keep the working distance fairly short ( {10mm)
to achieve acceptable image quality.

Hope this helps,
Ken Gaugler

Debby Sherman wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
}
} I have a student who wants to image magnetic particles with SEM. The
} particles are in the 400nm to 1µm size range. I had planned to stick them
} to double stick copper tape prior to imaging. However, I am concerned about
} their adversely affecting the microscope. Are there any suggestions as to
} mounting the particles, whether short working distance may magnify potential
} problems if some particles break loose and get into the lens. Etc?
}
} If there is a concern then I guess it would be possible to mount on
} sticky carbon tape, overlay the sample with a formvar film, and then use
} backscattering to image the particles. However, is this necessary?
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
}
}
}

--
ken-at-gaugler.com
(408) 296-4926





From daemon Sat Sep 6 11:33:23 2003



From: SPRINGSTEED,KRISTOPHER (HP-Corvallis,ex1) :      kristopher.springsteed-at-hp.com
Date: Sat, 6 Sep 2003 09:27:17 -0700
Subject: Re: SEM of Magnetic particles

Contents Retrieved from Microscopy Listserver Archives
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Wouldn't it also be dependent on the type of SEM used (i.e. HRSEM)?

If the final lens (pole piece) is not energized for through-the-lens
detection, the sticky Cu tape should hold them down. Disperse the particles
then blow off the weakly held particles. Now lower a ferro magnetic
material over the samples and see if any pop off. If particles do "pop" off
then use formvar and a high kV. The magnetism of the particles may play a
role in their physical construct so degauss with caution. I believe imaging
aberrations will most likely be the issue over that of tool contamination.
I have successfully imaged toner particles in a relatively low resolution
SEM. It should be noted that this tool has been classified as a "dirty"
tool. The toner particle were even dispersed on the "new" C sticky tabs.
The ones that are rock hard with barely any adhesive.

Kristopher



-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Friday, September 05, 2003 7:28 PM
To: Debby Sherman
Cc: MSA listserver


Is it possible to simply degauss the samples
before putting them in the SEM?

There is a border, I believe, between too
much degaussing and too little. Looking at
hard drive tracks is a case in point. Depending
on your ultimate resolution, the working distance
may facilitate your specimens by keeping them
away from the final lens. 1um specimens should
not require short WD.

Your BSE ought to be a separate issue depending
on what type it is and what KV it works at.

gary g.



At 03:14 PM 9/5/2003, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Sep 6 18:56:27 2003



From: Walck, Scott D. :      walck-at-ppg.com
Date: Sat, 6 Sep 2003 19:49:28 -0400
Subject: Package for measuring thickness contours

Contents Retrieved from Microscopy Listserver Archives
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You really don't need a package. You can do this by hand using basic TEM techniques.

I think that your best way to do this would be to set up a good 2-beam condition and use convergent beam electron diffraction. You use the distances between the symmetrical fringes (Kossel-Mollenstedt fringes) in the known reflection, calculate the deviation from exact Bragg condition for each fringe, and plot the results assuming that the first fringe that you see is the 1st, 2nd, or 3rd. You plot s(i)^2/n(i)^2 vs 1/n(i)^2, where n(i) is the index of the particular fringe. You try different starting values for n(1) starting from 1, then 2, then 3. You usually don't have to go higher than 3. When you get a straight line, the slope of the straight line is -1/(extdist)^2 and the intercept of the line is 1/t^2. This comes from the following relationship,

s(i)^2/n(i)^2 + 1/(extdist)^2/n(i)^2 = 1/t^2.

This technique is demonstrated in a number of TEM texts. The procedure is written in the Williams and Carter book.

You could apply this at two points on the sample along the wedge and get the angle.

You could also do this from the results of the two-beam approximation model. If you are at 2-beam Bright Field condition and you do not have bend contours present, then the first dark fringe that occurs in the wedge will occur at a thickness of 0.5 * (extdist(g)) and the second at 1.5 * (extdist(g)), (and so forth.) From the lateral separation distance between the fringes and the value of the extinction distance for that reflection, you can calculate the wedge angle. This is also written up in the Williams and Carter book and others as well.



-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: thurston e herricks [mailto:thurst0n-at-u.washington.edu]
Sent: Friday, September 05, 2003 3:37 PM
To: Microscopy-at-sparc5.microscopy.com


Hello all,

Does anyone have a package or know of a package that can calculate changes
in sample thickness from thickness fringes observed in conventional TEM?
I am imageing defect free single crystals on the order of 100nm. I need
to know how the crystals thickness profile changes.


Take care

Thurston Herricks







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