I can't find any information about a Sony Proscan camera. Is there another model name or number that is used for this camera?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: Thursday, January 29, 2004 15:47 To: microscopy-at-msa.microscopy.com
I had no idea that I would get such a great response for my precious tools of the trade. Thanks to everyone who called and e-mailed. The bad news is that someone in our microscope community has made me an offer I couldn't refuse (the cast comes off in two months). I'm really sorry that I couldn't help everyone out, but the deal is done.
} Date: Fri, 30 Jan 2004 13:16:30 -0500 } To: microscopy-at-ns.microscopy.com } From: John Grazul {grazul-at-ccmr.cornell.edu} } Subject: [Microscopy] Surplus equipment BLOWOUT!!!! } } If someone doesn't take this stuff its going to wind up as an artificial } reef in Lake Cayuga! } } Two Gatan Duo Mills, one working, one for your imagination! } } One Gatan PIPS system, it works to the best of my knowledge! } } One Gatan PIMS system, what can I say! I don't know, just enjoy it for } what it is! } } Two vintage VCR dimplers...they are unique! } } No reasonable offer will be refused! } } Will TRADE for an LKB glass knife maker! } } Remember, these items are a heartbeat away from a watery grave in Lake } Cayuga..... } } } If you are interested please e-mail or call } } John Grazul } Cornell University } 607 255 6421 } grazul-at-ccmr.cornell.edu
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 2 13:08:14 2004
750,000 cps would mean it would have to have an extraordinary detector and amplifier, not to mention a very short time constant. Shave off two zeros. Evex or someone made a typo. If this is true they should advertise.
"Excellent"? Is that a scalar or vector quantity?
Peter Tomic Agere Systems
-----Original Message----- } From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz] Sent: Monday, February 02, 2004 2:22 PM To: microscopy-at-msa.microscopy.com
Happy New Year
I recently received the email below from Evex.
This isn't for real, is it?
..........down to B? ..........750,000 cps????? ..........."excellent" (whatever that means) resolution??
Some of the new detector "chips" have gotten pretty fast. I have forgotten the exact values claimed by the makers of some newly announced detector chips so cannot comment directly. But...
1) Be sure that is *system* throughput, not just the detector spec. 2) Ask about the FWHM values at low/medium/high energy. 3) Ask about the peak/background ratio. 4) Think of other questions... Often, what the manufacturers *don't* tell you are the really important things ;)
Woody
\ Woody White BWXT Services: http://www.bwxt.com/bwxt.html My Site: http://woody.white.home.att.net
-----Original Message----- } From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz] Sent: Monday, February 02, 2004 2:22 PM To: microscopy-at-msa.microscopy.com
Happy New Year
I recently received the email below from Evex.
This isn't for real, is it?
..........down to B? ..........750,000 cps????? ..........."excellent" (whatever that means) resolution??
Dear Colleagues, Please refer this to interested microscopists. Thank-you.
The Cell Sciences Imaging Facility (CSIF) at Stanford University's Medical School is seeking a qualified electron microscopy technician. The position is a Life Sciences Research Assistant I (LSRAI). At least one year of experience working in an electron microscopy lab is desired and a minimum of an Associate Degree in the sciences is required for this position. Preference will be given to applicants with experience in biological electron microscopy. The position is currently listed as a 30 hours/week position but is being re-listed as a full time (40 hours/week) position. There is no money available for relocation. Stanford salary range is 2P1; actual salary is dependent upon experience and qualifications. Interested applicants can find more information at http://hrweb.stanford.edu/jobs/openings/display.cgi?Job_Req=004273&JFam=NIL&JOBCODE=5588 Please apply directly to:
Jon Mulholland, Director Cell Sciences Imaging Facility Beckman Center, B050 Stanford University School of Medicine Stanford, CA 94305-5301
fax 650-725-4951
jwm-at-stanford.edu http://taltos.stanford.edu
Jon Mulholland, Director Cell Sciences Imaging Facility Beckman Center, B050 Stanford University School of Medicine Stanford, CA 94305-5301
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 2 21:26:23 2004
Ritchie, Actually the numbers fit with the new detectors Rontec (I believe) has brought out. They made a presentation at NESM's May meeting in Woods Hole, MA. If I recall correctly, the resolution wasn't bad (140-160 eV maybe?) and the count rates are simply mind boggling. They also operate at considerably higher temperatures.
Ken Converse owner Quality Images third party SEM service Delta, PA
Ritchie Sims wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 3 10:52:45 2004
Dear Colleagues, I am re-posting this because Stanford HR has changed the job # and URL. Please refer this to interested microscopists. Thank-you.
The Cell Sciences Imaging Facility (CSIF) at Stanford University's Medical School is seeking a qualified electron microscopy technician. The position is a Life Sciences Research Assistant I (LSRAI). At least one year of experience working in an electron microscopy lab is desired and a minimum of an Associate Degree in the sciences is required for this position. Preference will be given to applicants with experience in biological electron microscopy. The position is a full time (40 hours/week) position. There is no money available for relocation. Stanford salary range is 2P1; actual salary is dependent upon experience and qualifications. Interested applicants can find more information at http://hrweb.stanford.edu/jobs/openings/display.cgi?Job_Req=004773&JFam=NIL&JOBCODE=5588 Please apply directly to:
Jon Mulholland, Director Cell Sciences Imaging Facility Beckman Center, B050 Stanford University School of Medicine Stanford, CA 94305-5301
fax 650-725-4951
jwm-at-stanford.edu http://taltos.stanford.edu
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 3 11:11:36 2004
We have been using a Nikon Coolpix 990 for several years now and subsequently added Coolpix 4500. Other than issues with chromatic aberration, the micrographs are quite usable.
We occasionally experience problems with being unable to retrieve our images off of the compact flash cards. Files are written to the compact flash cards as verified by looking at the properties (unused/used space), however, there are no identifiable files present. Consequently, we reformat the card and have to reshoot our photos. This is not only inconvenient, but sometimes the samples have to be re-prepared or an unruly crowd has to sit and wait.
This occurs with both the 990 and 4500 cameras as well as various brands of cards and card readers (Lexar and SanDisk). It is not isolated to a particular computer nor a particular user. Nikon tech support has never heard of anyone else having this problem.
Anyone out there with similar experiences?
Thanks.
Alan Stone ASTON Metallurgical Services
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 3 12:19:27 2004
Alan; I had a similar problem with a Sony camera using Memory Stick: If I used the computer to manage the memory, i.e. clearing the memory of old photos or formatting the card with the computer, the card would become corrupted. If I use the CAMERA to reformat the card, I never have any problems.
John Mardinly Intel
-----Original Message----- } From: Alan Stone [mailto:as-at-astonmet.com] Sent: Tuesday, February 03, 2004 9:22 AM To: microscopy-at-msa.microscopy.com
I've used a Coolpix for some time now, as well as several other cameras from Canon, Pentax and Sony that use CF, memory stick and SD flash memory. With all of them I've found that formatting the memory in the camera is the best solution. In my case, I sometimes read the memory in a Mac and sometimes in a PC. If I format the memory in the camera, both operating systems will read the files without difficulty. If the memory is formatted in the computer, the camera usually sees it OK but the other computer system sometimes has difficulties.
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 3 14:20:48 2004
John W. Raffensperger, Jr. IS Manager Helwig Carbon Products, Inc.
-----Original Message----- } From: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: Tuesday, February 03, 2004 12:30 PM To: Alan Stone; microscopy-at-msa.microscopy.com
Alan,
Two questions. 1) Can you see the 'missing' images using the camera's playback function ('defective' CF card in camera)? 2) If so, can you download those images from the camera to your computer via the USB cable?
If the answer to above questions is yes than you may have an intermittent computer/CF card management problem. I always format my cards in the camera. CF cards do fail of course but I have taken over 1000 pix using Lexar and Transcend cards on a Canon G3. No lost images yet. Good luck, Jim
} Date: Tue, 03 Feb 2004 11:22:24 -0600 } To: microscopy-at-msa.microscopy.com } From: Alan Stone {as-at-astonmet.com} } Subject: [Microscopy] Coolpix Compact Flash Problems
} } } We have been using a Nikon Coolpix 990 for several years now and } subsequently added Coolpix 4500. Other than issues with chromatic } aberration, the micrographs are quite usable. } } We occasionally experience problems with being unable to retrieve our } images off of the compact flash cards. Files are written to the compact } flash cards as verified by looking at the properties (unused/used space), } however, there are no identifiable files present. Consequently, we reformat } the card and have to reshoot our photos. This is not only inconvenient, but } sometimes the samples have to be re-prepared or an unruly crowd has to sit } and wait. } } This occurs with both the 990 and 4500 cameras as well as various brands of } cards and card readers (Lexar and SanDisk). It is not isolated to a } particular computer nor a particular user. Nikon tech support has never } heard of anyone else having this problem. } } Anyone out there with similar experiences? } } Thanks. } } Alan Stone } ASTON Metallurgical Services }
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility Unit 3242 91 North Eagleville Road BSP Building, Room G06 Storrs, CT 06269-3242
I want to clarify that all of the formatting and re-formatting has been done directly in the Coolpix cameras and never in the computers.
This is such an erratic problem that we don't see any patterns. It appears to be some type of index writing problem, maybe the sessions are not properly closing out. We added time between turning off the camera and removing the chip to ensure that we give the camera enough time. It did not help. It also is unrelated to how many pictures have been taken, whether the card is new, used or old.
We will be doing a trial to keep the cards from being mixed between the different cameras. I don't believe that is an issue as we have had failures shortly after re-formatting.
If you do had similar problems, then please contact Nikon's tech support. Again, they tell me I am the only one reporting this.
Regards,
Alan Stone ASTON
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 3 15:47:20 2004
Formatting in camera is generally a good idea. If you must format in a PC with Windows XP or 2000, make sure you format the card using "FAT" and not "FAT 32". With the exception of a few new high end models, most cameras will not recognize media formatted FAT 32.
George
George Laing National Graphic Supply 800.223.7130 x3109 scisales-at-ngscorp.com
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 3 18:16:32 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kemmons-at-wrfseattle.org) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, February 3, 2004 at 13:19:57 ---------------------------------------------------------------------------
Question: Can anyone tell me what the installed base of scanning and/or transmission electron microscopes is in the U.S. or worldwide, or otherwise point me to a resource which can do so?
I am also intersted in statistics on the number of samples processed for a given period of time on the average machine, but I suspect it varies widely from one user to the next. If anyone would like to volunteer information from their own experience, it would be helpful.
the only other suggestion that I can make is that maybe the camera has still been powered up when you have removed the card for reading, because I know that there have been reports that this can cause problems.
Malcolm
Malcolm Haswell e.m. unit University of Sunderland UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- } From: Alan Stone {as-at-astonmet.com}
Focus on Microscopy 2004 Philadelphia, USA, April 4 to April 7, 2004
Dear Colleagues,
we have received a number of inquiries whether if it would still be possible to submit abstracts to FOM2004. To accommodate these requests we have extended the deadline to Friday, February 13. However, this deadline will be strictly observed since we aim to have the program ready and inform you about acceptance at the end of the week after that.
Please use for submission our electronic system at http://www.FocusOnMicroscopy.org/2004/abstracts.html and submit until February 13.
Contributions will be selected for either key note presentation, regular contribution or poster.
For further information see http://www.FocusOnMicroscopy.org
On behalf of the organizers FOM2004
Andres Kriete Drexel University & Coriell Institute Philadelphia, USA G.J. (Fred) Brakenhoff Univ. of Amsterdam, the Netherlands
Yes Alan I have experienced this problem. Unfortunately for me this occurred in the middle of a failure analysis while cross sectioning a defective capacitor. I lost some irreplaceable pictures.
Anyway I as usual turned the camera off and waited 15 seconds and removed the card from the camera inserted into the card reader connected to the computer. At this point I move the images to the hard drive on my computer. After this I manipulate the images from the hard drive. The problem encountered is removing the card from the reader. If removed too soon it will cause either the next card inserted to be corrupt or the card removed will be corrupt (no images). Now do not get the impression I am removing the card while the data is transferring but some 15 to 30 seconds after the transfer. So the solution I have come up with is to keep 2 cards on hand so that I can insert and leave one in the reader for some time, at least a few minutes to avoid this drastic situation. Hope this helps with your situation.
Robert Fowler Quality Assurance Failure Analysis Technician TDK Components USA, Inc. Multilayer Ceramic Capacitor Division 1 TDK Boulevard Peachtree City GA 30269-2051 Telephone: (770) 631-0410 Ext.249 Fax: (770) 487-1460 email: robert.fowler-at-tdktca.com www.component.tdk.com
Alan Stone {as-at-astonmet.com} To 02/04/2004 08:47 AM Robert.Fowler-at-tdktca.com cc
Subject Re: Fw: Follow Up to Coolpix Compact Flash Posting
Robert,
We initially format the cards in the cameras or re-format after a card failure. Our routine is to take our pictures, turn off the cameras & wait a minimum of 15 seconds. We then remove the cards, bring them over to our desktop computers and insert the cards into the card readers. Occasionally, the images are not retrievable. After not having a problem for several months, I had three failures last week while hosting a legal project and then twice again yesterday and on Monday. We cannot pin this down to a particular card, camera, computer, user nor card reader.
Alan
At 07:26 AM 2/4/2004, you wrote:
} Alan are you using a card reader on the computer? If this is the case I } will send a response to the listserver. } } Robert Fowler } Quality Assurance Failure Analysis Technician } TDK Components USA, Inc. } Multilayer Ceramic Capacitor Division } 1 TDK Boulevard } Peachtree City GA 30269-2051 } Telephone: (770) 631-0410 Ext.249 } Fax: (770) 487-1460 } email: robert.fowler-at-tdktca.com } www.component.tdk.com } ----- Forwarded by Robert Fowler/TCU/TDK-US on 02/04/2004 08:24 AM ----- } } Alan Stone } {as-at-astonmet.com} } To } 02/03/2004 04:14 PM microscopy-at-msa.microscopy.com } cc } } Subject } Follow Up to Coolpix } Compact Flash Posting } } } } } } } } } } } } } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } } I want to clarify that all of the formatting and re-formatting has been } done directly in the Coolpix cameras and never in the computers. } } This is such an erratic problem that we don't see any patterns. It appears } to be some type of index writing problem, maybe the sessions are not } properly closing out. We added time between turning off the camera and } removing the chip to ensure that we give the camera enough time. It did not } help. It also is unrelated to how many pictures have been taken, whether } the card is new, used or old. } } We will be doing a trial to keep the cards from being mixed between the } different cameras. I don't believe that is an issue as we have had failures } shortly after re-formatting. } } If you do had similar problems, then please contact Nikon's tech support. } Again, they tell me I am the only one reporting this. } } Regards, } } Alan Stone } ASTON } } } } } } } THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY } TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL } INFORMATION. } } If you are not the intended recipient, be advised that any use, } dissemination, distribution or duplication of this transmission is strictly } prohibited. If you received this transmission in error, please notify the } sender immediately by electronic reply to this transmission or by phone } (847-803-6100). Thank you.
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 08:51:57 2004
Hi Listers, I am interested in measuring quantitatively the chitin (N-acetylglucosamin) in fungal cell wall (Aspergillus spp.). We are looking for antibody(ies)(monoclonal or polyclonal) against chitin. I would very much appreciate any clue.
Haixin Xu (PhD) Dept of Biological Sciences University of Maryland Baltimore County Baltimore, MD 21228
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 09:11:49 2004
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Yes Alan I have experienced this problem. Unfortunately for me this occurred in the middle of a failure analysis while cross sectioning a defective capacitor. I lost some irreplaceable pictures.
Anyway I as usual turned the camera off and waited 15 seconds and removed the card from the camera inserted into the card reader connected to the computer. At this point I move the images to the hard drive on my computer. After this I manipulate the images from the hard drive. The problem encountered is removing the card from the reader. If removed too soon it will cause either the next card inserted to be corrupt or the card removed will be corrupt (no images). Now do not get the impression I am removing the card while the data is transferring but some 15 to 30 seconds after the transfer. So the solution I have come up with is to keep 2 cards on hand so that I can insert and leave one in the reader for some time, at least a few minutes to avoid this drastic situation. Hope this helps with your situation.
Robert Fowler Quality Assurance Failure Analysis Technician TDK Components USA, Inc. Multilayer Ceramic Capacitor Division 1 TDK Boulevard Peachtree City GA 30269-2051 Telephone: (770) 631-0410 Ext.249 Fax: (770) 487-1460 email: robert.fowler-at-tdktca.com www.component.tdk.com
Alan Stone {as-at-astonmet.com} To 02/04/2004 08:47 AM Robert.Fowler-at-tdktca.com cc
Subject Re: Fw: Follow Up to Coolpix Compact Flash Posting
Robert,
We initially format the cards in the cameras or re-format after a card failure. Our routine is to take our pictures, turn off the cameras & wait a minimum of 15 seconds. We then remove the cards, bring them over to our desktop computers and insert the cards into the card readers. Occasionally, the images are not retrievable. After not having a problem for several months, I had three failures last week while hosting a legal project and then twice again yesterday and on Monday. We cannot pin this down to a particular card, camera, computer, user nor card reader.
Alan
At 07:26 AM 2/4/2004, you wrote:
} Alan are you using a card reader on the computer? If this is the case I } will send a response to the listserver. } } Robert Fowler } Quality Assurance Failure Analysis Technician } TDK Components USA, Inc. } Multilayer Ceramic Capacitor Division } 1 TDK Boulevard } Peachtree City GA 30269-2051 } Telephone: (770) 631-0410 Ext.249 } Fax: (770) 487-1460 } email: robert.fowler-at-tdktca.com } www.component.tdk.com } ----- Forwarded by Robert Fowler/TCU/TDK-US on 02/04/2004 08:24 AM ----- } } Alan Stone } {as-at-astonmet.com} } To } 02/03/2004 04:14 PM microscopy-at-msa.microscopy.com } cc } } Subject } Follow Up to Coolpix } Compact Flash Posting } } } } } } } } } } } } } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } } I want to clarify that all of the formatting and re-formatting has been } done directly in the Coolpix cameras and never in the computers. } } This is such an erratic problem that we don't see any patterns. It appears } to be some type of index writing problem, maybe the sessions are not } properly closing out. We added time between turning off the camera and } removing the chip to ensure that we give the camera enough time. It did not } help. It also is unrelated to how many pictures have been taken, whether } the card is new, used or old. } } We will be doing a trial to keep the cards from being mixed between the } different cameras. I don't believe that is an issue as we have had failures } shortly after re-formatting. } } If you do had similar problems, then please contact Nikon's tech support. } Again, they tell me I am the only one reporting this. } } Regards, } } Alan Stone } ASTON } } } } } } } THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY } TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL } INFORMATION. } } If you are not the intended recipient, be advised that any use, } dissemination, distribution or duplication of this transmission is strictly } prohibited. If you received this transmission in error, please notify the } sender immediately by electronic reply to this transmission or by phone } (847-803-6100). Thank you.
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 13:01:05 2004
We are in the final stages of acquiring a new scope. I am looking for comments, feedback, etc. - the good, the bad and the ugly - from users/owners/managers of FEI Quanta (ESEM) FEG scopes.
Best done with direct emailings to me rather than back to the list. Or if you would like I would be happy to call anyone wishing to talk rather than email.
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 350 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 13:03:27 2004
On Feb 4, 2004, at 9:44 AM, Christopher Gilpin wrote:
} I was wondering what people were using to pump the dewars of TEM cryo } holders. I have been using a line to a standard Denton coating unit but } this is becoming impractical. I am aware of dedicated solutions as } produced by Gatan but would be interested in how creative users have } been. } Dear Chris, We had a set-up on the HVEM with lines from the airlock mechanical pump and the column high-vacuum pump that was used to pump out the dewars of the cryo holder and the GATAN anticontaminator. We did not experience any microscope vacuum problems as a result of using this system, although the column vacuum was not as high as that in many more-modern TEMs. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 13:40:21 2004
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Yes Alan I have experienced this problem. Unfortunately for me this occurred in the middle of a failure analysis while cross sectioning a defective capacitor. I lost some irreplaceable pictures.
Anyway I as usual turned the camera off and waited 15 seconds and removed the card from the camera inserted into the card reader connected to the computer. At this point I move the images to the hard drive on my computer. After this I manipulate the images from the hard drive. The problem encountered is removing the card from the reader. If removed too soon it will cause either the next card inserted to be corrupt or the card removed will be corrupt (no images). Now do not get the impression I am removing the card while the data is transferring but some 15 to 30 seconds after the transfer. So the solution I have come up with is to keep 2 cards on hand so that I can insert and leave one in the reader for some time, at least a few minutes to avoid this drastic situation. Hope this helps with your situation.
Robert Fowler Quality Assurance Failure Analysis Technician TDK Components USA, Inc. Multilayer Ceramic Capacitor Division 1 TDK Boulevard Peachtree City GA 30269-2051 Telephone: (770) 631-0410 Ext.249 Fax: (770) 487-1460 email: robert.fowler-at-tdktca.com www.component.tdk.com
Alan Stone {as-at-astonmet.com} To 02/04/2004 08:47 AM Robert.Fowler-at-tdktca.com cc
Subject Re: Fw: Follow Up to Coolpix Compact Flash Posting
Robert,
We initially format the cards in the cameras or re-format after a card failure. Our routine is to take our pictures, turn off the cameras & wait a minimum of 15 seconds. We then remove the cards, bring them over to our desktop computers and insert the cards into the card readers. Occasionally, the images are not retrievable. After not having a problem for several months, I had three failures last week while hosting a legal project and then twice again yesterday and on Monday. We cannot pin this down to a particular card, camera, computer, user nor card reader.
Alan
At 07:26 AM 2/4/2004, you wrote:
} Alan are you using a card reader on the computer? If this is the case I } will send a response to the listserver. } } Robert Fowler } Quality Assurance Failure Analysis Technician } TDK Components USA, Inc. } Multilayer Ceramic Capacitor Division } 1 TDK Boulevard } Peachtree City GA 30269-2051 } Telephone: (770) 631-0410 Ext.249 } Fax: (770) 487-1460 } email: robert.fowler-at-tdktca.com } www.component.tdk.com } ----- Forwarded by Robert Fowler/TCU/TDK-US on 02/04/2004 08:24 AM ----- } } Alan Stone } {as-at-astonmet.com} } To } 02/03/2004 04:14 PM microscopy-at-msa.microscopy.com } cc } } Subject } Follow Up to Coolpix } Compact Flash Posting } } } } } } } } } } } } } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } } I want to clarify that all of the formatting and re-formatting has been } done directly in the Coolpix cameras and never in the computers. } } This is such an erratic problem that we don't see any patterns. It appears } to be some type of index writing problem, maybe the sessions are not } properly closing out. We added time between turning off the camera and } removing the chip to ensure that we give the camera enough time. It did not } help. It also is unrelated to how many pictures have been taken, whether } the card is new, used or old. } } We will be doing a trial to keep the cards from being mixed between the } different cameras. I don't believe that is an issue as we have had failures } shortly after re-formatting. } } If you do had similar problems, then please contact Nikon's tech support. } Again, they tell me I am the only one reporting this. } } Regards, } } Alan Stone } ASTON } } } } } } } THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY } TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL } INFORMATION. } } If you are not the intended recipient, be advised that any use, } dissemination, distribution or duplication of this transmission is strictly } prohibited. If you received this transmission in error, please notify the } sender immediately by electronic reply to this transmission or by phone } (847-803-6100). Thank you.
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 14:04:27 2004
I've inherited an MT-5000 ultramicrotome that hasn't been used in a number of years. RMC/Boeckler won't offer service on this model. Some parts are still available.
Does anyone know who does microtome repair in the Seattle, Washington area?
Thanks,
Paulette Brunner Center for Cell Dynamics Friday Harbor Labs, University of Washington
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi Listers, I am interested in measuring quantitatively the chitin (N-acetylglucosamin) in fungal cell wall (Aspergillus spp.). We are looking for antibody(ies)(monoclonal or polyclonal) against chitin. I would very much appreciate any clue.
Haixin Xu (PhD) Dept of Biological Sciences University of Maryland Baltimore County Baltimore, MD 21228
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 14:41:42 2004
Did you try searching through abcam? This is the link: http://www.abcam.com/
Cheers,
Jacqui.
Haixin Xu wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } Hi Listers, } I am interested in measuring quantitatively the chitin } (N-acetylglucosamin) in fungal cell wall (Aspergillus spp.). We are } looking for antibody(ies)(monoclonal or polyclonal) against chitin. I } would very much appreciate any clue. } } Haixin Xu (PhD) } Dept of Biological Sciences } University of Maryland Baltimore County } Baltimore, MD 21228
Jacqueline Ross Biomedical Imaging Research Unit Division of Anatomy with Radiology Faculty of Medical & Health Sciences The University of Auckland Private Bag 92019 Auckland, NEW ZEALAND
Tel: 64 9 373 7599 Ext 7438 Fax: 64 9 373 7484
http://www.health.auckland.ac.nz/biru/
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 15:16:21 2004
Listers: I've got a Spicer Consulting SC12 Magnetic Field Canceling System, but no manual to go with it. If anybody on the list has one they will loan me or copy for me? I will be glad to pay postage and/or copy costs. Please reply off-list.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 15:59:43 2004
A friend has sent me a really nice calendar as a late Christmas present. It's got photos of old light microscopes, micrographs (all pathology), microscopes on postage stamps, and microscopy-related quotations (some are from the Project MICRO collection). The price is right - it's free. Here's the description:
The National Museum of Health and Medicine of the Armed Forces Institute of Pathology in Washington, D.C. is offering the 2004 American Registry of Pathology/Armed Forces Institute of Pathology calendar on a first-come, first-served basis. The calendar features photographs of the microscopes in the museum's Billings Microscope Collection. For a free copy, send your name, address, and affiliation by e-mail only to nmhminfo-at-afip.osd.mil. Requests for multiple copies cannot be accommodated. -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/ Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 16:31:55 2004
I'm sorry if someone has already suggested this, but why don't you just take the camera to the computer, or vice-versa, and download off the camera with the Nikon USB cable?
Maybe not everyone's setup allows this, but we've been running a Coolpix 4500 for some months now, we never remove the card from the camera, and we've never had any problems at all. The nikon download s/w kicks in automatically and works a treat.
cheers
rtch
Tech Forum Professional Technical Staff Awards (PTSA)
Are you an MSA member and planning to submit an abstract for M & M 2004 in Savannah? If so, consider submitting an application for the Tech Forum's PTSA. This award consists of complimentary full meeting registration and travel reimbursement up to $600.00. The requirements are simple: 1. submit a paper/poster abstract at the meeting website, 2. send a copy of the abstract to the TF Chair at the address below, and 3. send a support letter from your manager/supervisor confirming your position as full-time technical support staff. Deadline is February 16, 2004. Either hardcopy or electronic copy of abstract & support letter will be accepted.
Submit Applications to:
Cathy Johnson TF Chair cjohnmicro-at-aol.com 6422 Simms St. #66 Arvada, CO 80004 (303) 420-6373
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 4 17:28:32 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rpowell-at-nanoprobes.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 4, 2004 at 12:09:24 ---------------------------------------------------------------------------
Email: rpowell-at-nanoprobes.com Name: Rick Powell at Nanoprobes
Organization: Nanoprobes, Incorporated
Title-Subject: [Microscopy] searching for antibody to chitin
Question: Hello Haixin:
Can't recommend any specific antibodies, but the best place to look is Linscott's Directory of Immunological and Biological Reagents:
http://www.linscottsdirectory.com/
USA: 4877 Grange Road Santa Rosa, CA 95404 Tel: (707) 763-7098 Fax: (707) 763-8372
You could also try Abcam (may need to register first):
http://www.abcam.com/
Hope this helps,
Rick Powell
***************************************************************************************** Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com NANOPROBES, Incorporated 95 Horse Block Road, Yaphank, NY 11980-9710, USA
Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html *****************************************************************************************
I must be wacky.... I remember prepping chloroform suspensions on carbon coated formvar copper grids without any problems 5 years ago. Now my films are dissolving and my particles left behind. Has the polymer structure of "formvar" changed? I prefer to use chloroform for my suspensions, so does this means I must resign myself to the more fragile (I can be pretty ham-handed at times) carbon film grids?
Suggestion and advice are welcome!!!
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 5 13:40:57 2004
Hi, We use formvar in diethylene chloride all the time and have no problem. We use them for students because it is so durable. Generally for standard 80 or 100 kV we use 0.25% formvar in diethylene chloride. Good luck, Judy
Judy Murphy, PhD San Joaquin Delta College Microscopy Technology 5151 Pacific Ave Stockton, CA 95207 209-954-5284 FAX 209-954-5600 jmurphy-at-deltacollege.edu http://www.sjdccd.cc.ca.us/dept/electmicro/index.html
Frank.Karl-at-degussa.com wrote: } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } I must be wacky.... I remember prepping chloroform suspensions on carbon } coated formvar copper grids without any problems 5 years ago. Now my films } are dissolving and my particles left behind. Has the polymer structure of } "formvar" changed? I prefer to use chloroform for my suspensions, so does } this means I must resign myself to the more fragile (I can be pretty } ham-handed at times) carbon film grids? } } Suggestion and advice are welcome!!! } } Frank Karl } Degussa Corporation } Akron Technical Center } 3500 Embassy Parkway } Suite 100 } Akron, Ohio 44333 } } 330-668-2235 Ext. 238
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 5 14:15:02 2004
I haven't done formvar grids in a long time, but when I did, I also made them up in diethylene chloride.
Peggy Sherwood Lab Associate, Photopathology Wellman Laboratories of Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
-----Original Message----- } From: Judy Murphy [mailto:jmurphy-at-deltacollege.org] Sent: Thursday, February 05, 2004 2:54 PM To: Frank.Karl-at-degussa.com Cc: microscopy-at-msa.microscopy.com
Hi, We use formvar in diethylene chloride all the time and have no problem. We use them for students because it is so durable. Generally for standard 80 or 100 kV we use 0.25% formvar in diethylene chloride. Good luck, Judy
Judy Murphy, PhD San Joaquin Delta College Microscopy Technology 5151 Pacific Ave Stockton, CA 95207 209-954-5284 FAX 209-954-5600 jmurphy-at-deltacollege.edu http://www.sjdccd.cc.ca.us/dept/electmicro/index.html
Frank.Karl-at-degussa.com wrote: } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } I must be wacky.... I remember prepping chloroform suspensions on carbon } coated formvar copper grids without any problems 5 years ago. Now my films } are dissolving and my particles left behind. Has the polymer structure of } "formvar" changed? I prefer to use chloroform for my suspensions, so does } this means I must resign myself to the more fragile (I can be pretty } ham-handed at times) carbon film grids? } } Suggestion and advice are welcome!!! } } Frank Karl } Degussa Corporation } Akron Technical Center } 3500 Embassy Parkway } Suite 100 } Akron, Ohio 44333 } } 330-668-2235 Ext. 238
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 5 14:43:40 2004
Long ago we did use chloroform as a solvent for Formvar, so I'm not surprised that when you apply your suspensions in chloroform the Formvar breaks or dissolves. I'm betting that 1) you've forgotten that you had to switch to something other than chloroform, 2) you had thicker Formvar and a smaller volume of chloroform that you left on for less time, or 3) most likely, you used carbon-stabilized Formvar coated grids and the chloroform did not get to the Formvar. Try the latter, making sure you put your suspensions on the carbon side.
Good luck!
Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 5 14:56:20 2004
} At 12:33 PM 1/31/2004, you wrote: } } } I think the short answer will be that such materials don't } } } diffract. The spacings are in a whole other realm of size. You } } } would need a much longer wavelength to get diffraction. Also, the } } } spacings are not so regular as to be able to generate anything } } } like planes of atoms. "Peaks" would likely be poorly defined, but } } } variation in their shape as well as position might lend some } } } usable information. } } } } } } Warren } } } } I hate to disagree but not only can you do electron diffraction in } } a TEM from such structures, I have done it myself. It is even } } possible to get a diffraction pattern from the bars of a grid. } } } } As an alternative to electron diffraction, you can do light } } diffraction from the negative or do an FFT from a digital image. } } } } In all cases, you very quickly get information on the average } } spacing of regular structures and avoid a lot of tedious } } measurements. } } } } Regards, } } -- } } Larry Stoter } Point taken. I was thinking x-ray diffraction more than other } methods. I would still wonder how well diffraction works with only } semi-regular arrangement, but I suppose some diffraction pattern } should result with any periodicity. The challenge is finding a beam } of the right wavelength. } } I have not played around with FFTs on images yet. Most of our } samples are not near that regular, but I may have to look into it. } } Warren } I must say, I don't understand the physics but, in a TEM, you really can get electron diffraction from periodic structures which are many order of magnitude larger than any wavelength involved (although, atomic spacings are quite abit larger than the electron wavelength anyway). And the regularity doesn't need to be that good - muscle can give some very nice diffraction patterns.
Going back to the orginal question, why isn't electron diffraction used to measure spacings in periodic biological structures - muscle and myelin being the two most obvious.
regards, -- Larry Stoter NOTE - any message other than plain text will be automatically deleted :-)
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 5 15:01:18 2004
Listers: One of our colleagues was nice enough to fax me a copy of his manual, so I'm all set. Thanks for the replies. This list is a great asset. Way to go, Nestor!
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } Listers: I've got a Spicer Consulting SC12 Magnetic Field } Canceling System, but no manual to go with it. If anybody } on the list has one they will loan me or copy for me? I } will be glad to pay postage and/or copy costs. Please reply } off-list. } } -- } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Becky Holdford (r-holdford-at-ti.com) } 972-995-2360 } 972-648-8743 (pager) } SC Packaging FA Development } Texas Instruments, Inc. } Dallas, TX } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 5 15:42:19 2004
We would recommend a pure carbon coated grid where all the traces of plastic are removed. We produce them for non-aqueous solutions so solvents will not harm the carbon support film. This would eliminate the dissolving of the formvar by the solvent.
John Arnott
Disclaimer: Ladd Research sell carbon coated grids that are described in this e-mail
Ladd Research 83 Holly Court Williston, VT 05495
On-line Catalog: http://www.laddresearch.com
tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US) fax: 1-802-660-8859 e-mail: sales-at-laddresearch.com ----- Original Message ----- } From: "Judy Murphy" {jmurphy-at-deltacollege.org} To: {Frank.Karl-at-degussa.com} Cc: {microscopy-at-msa.microscopy.com} Sent: Thursday, February 05, 2004 2:53 PM
Hi, all
A word from a light microscopist: Diffraction happens all the time. You can even do a neat diffraction experiment with your hand. If you hold your hand between your eye and a distant light source (palm toward you seems to work best, looking at, for instance, the ceiling light) and leave just a small space between your fingers, you will see the bright/dark/bright fringes which result from the interference of light diffracting around your fingers.
Using diffraction patterns to measure periodic structures is well known in interferometry. I have measured structures below the limit of resolution by calibrating a special eyepiece micrometer (ex: a "Wright" eyepiece) and viewing the diffraction pattern of a slightly sheared image in the back focal plane of the objective (many many years ago, now).
I also used X-ray diffraction to measure spacing in liquid crystals (also many years ago).
The challenge is to get enough of the periodic structure so that its diffraction pattern is not washed out by the overlaying of the diffraction patterns from other, non-periodic structures.
All of this is based in fundamental physics (Young's far-field diffraction experiment, and Bragg's Law).
For anyone interested in trying... let me know how the experiment comes out!
Best regards, Barbara Foster Microscopy/Microscopy Education 125 Paridon Street, Suite 102 Springfield, MA 01118 PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^ Don't forget: the American Chem Society's Applied Optical Microscopy Course, March 5-7, Chicago, Ill. Visit our website for details and registration. NOT JUST FOR CHEMISTS!!!! ^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^
At 09:00 PM 2/5/04 +0000, Larry Stoter wrote:
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From MicroscopyL-request-at-ns.microscopy.com Thu Feb 5 16:07:58 2004
Although most people use ethylene dichloride for Formvar, chloroform will also dissolve Butvar and Formvar. Some companies, in fact, provide various concentrations of the plastic dissolved in chloroform. We use 0.25% Butvar in chloroform.
I believe that what you are experiencing is, indeed, the dissolution of the underlying Formvar layer leaving a very fragile carbon layer that breaks upon drying of your particulate suspension. So, either change the liquid you are suspending the particles in (try methanol, acentone) or put a heavier layer of carbon on the Formvar.
} I must be wacky.... I remember prepping chloroform suspensions on carbon } coated formvar copper grids without any problems 5 years ago. Now my films } are dissolving and my particles left behind. Has the polymer structure of } "formvar" changed? I prefer to use chloroform for my suspensions, so does } this means I must resign myself to the more fragile (I can be pretty } ham-handed at times) carbon film grids? } } Suggestion and advice are welcome!!! } } Frank Karl } Degussa Corporation
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu ##############################################################
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 5 17:29:48 2004
Dear Listers: I recently ordered Epofix as an alternative to LR White, since it supposedly cures at RT and is purported not to infiltrate samples. After polymerizing in various molds, at low pressure, waiting for days, I do not get blocks hard enough for ultramicrotomy. After numerous emails to the manufacturer, the gist is that it DOES require some heat to get a hard block (one that a fingernail doesn't go through). I found a post from about a year ago mentioning Epofix; I am wondering if anyone does get this resin to harden at room temp? Thanks, Jessica
Bend Research, Inc 64550 Research Rd Bend, OR 97701 (541) 382-4100 page (541) 382-0212 x240
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 6 01:22:48 2004
Hi Jessica, I have been using Epofix resin without problems. It hardens at 22ºC in about 24 hours. You have to control carefully the proportion of resin and hardener. I mix 15 parts by volume of resin with 2 parts by volume of hardener and I get blocks hard enough for ultramicrotomy. I buy Epofix to EMS (Electron Microscopy Science). Cristina Almansa University of Alicante SPAIN jcervantes-at-bendres.com ha escrito:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } Dear Listers: } I recently ordered Epofix as an alternative to LR White, since it supposedly } cures at RT and is purported not to infiltrate samples. After polymerizing } in various molds, at low pressure, waiting for days, I do not get blocks } hard enough for ultramicrotomy. After numerous emails to the manufacturer, } the gist is that it DOES require some heat to get a hard block (one that a } fingernail doesn't go through). I found a post from about a year ago } mentioning Epofix; I am wondering if anyone does get this resin to harden at } room temp? } Thanks, } Jessica } } Bend Research, Inc } 64550 Research Rd } Bend, OR 97701 } (541) 382-4100 page } (541) 382-0212 x240
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 6 02:02:15 2004
I'm having serious problems achieving adequate development of the new Kodak 4489 EM film. We have no facilities to carry out development on site and have to send film packages to another lab, subsequently, the quality of development is at the discretion of the other (non-commercial) lab. Basically its a hit or miss even using new development protocols. Its got to the stage Id be willing to send our film anywhere in the UK (maybe Europe) to be developed commercially if consistently good quality can be achieved. Does anyone know of somewhere which offers this service?
Thanks.
Veterinary Laboratories Agency (VLA)
This email and any attachments is intended for the named recipient only. Its unauthorised use, disclosure, storage or copying is not permitted. If you have received it in error, please destroy all copies and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes.
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 6 05:32:48 2004
We use a Cameca SU-30 as SEM and need to coat some fresh fruits with carbon. Can anyone send me an information about coating and sample preparation instructions for organic materials? Thanks.
Orkun ERSOY
Hacettepe University
Geological Engineering
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 6 10:06:02 2004
Thanks to all the replies on Epofix. It is definitely true that I am using a much smaller volume than the plugs or mounts mentioned by some of you, so the temperature of the resin never rises above RT (I did measure this, looking for an exotherm). I will try larger molds and heating slightly as suggested; thanks to everyone again for being a good resource.
Jessica Cervantes Bend Research, Inc 64550 Research Rd Bend, OR 97701 (541) 382-4100 page
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 6 12:42:10 2004
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Thanks to all the replies on Epofix. It is definitely true that I am using a much smaller volume than the plugs or mounts mentioned by some of you, so the temperature of the resin never rises above RT (I did measure this, looking for an exotherm). I will try larger molds and heating slightly as suggested; thanks to everyone again for being a good resource.
Jessica Cervantes Bend Research, Inc 64550 Research Rd Bend, OR 97701 (541) 382-4100 page
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 6 13:37:22 2004
The 2004 Spring Meeting of THE TEXAS SOCIETY FOR MICROSCOPY “Embracing All Forms of Microscopy”
April 22, 23, 24, 2004
ABSTRACTS MUST BE RECEIVED BY: MARCH 11, 2004 Mail abstracts to: Camelia G.-A. Maier, Ph.D. Department of Biology Texas Woman's University Denton, TX 76204-5799 Tel.: 940-898-2358 (office) E-mail: cmaier-at-twu.edu
The Doubletree Hotel - Post Oak 2001 Post Oak Blvd. Houston, TX 77056 (713)961-9300 (800)245-7299
RATES: $85.00 Single, Double, Triple and Quad Mention that you are with the Texas Society for Microscopy when making your reservations.
HOTEL RESERVATION DEADLINE: MARCH 25, 2004 After this date reservations will be accepted on a space available basis, convention rates not guaranteed.
THURSDAY WORKSHOP Spectrum Imaging for Materials Characterization Presented by John J. Friel Princeton Gamma - Tech
Please see our website (http://www.texasmicroscopy.org) for registration forms and author's instructions.
our group is studying interaction of microbial cells (yeast and bacteria) to several kinds of biomaterials. We conduct physico/chemical analysis of the involved surfaces (mainly throgh surface thermodynamical analysis: contact angles with several liquids) and have also an atomic force microscopy for high resolution mapping of the surface topography and force measurements.
At this moment, we are interested in seeing what is happening with the contact side of the cell with the substratum. For this reason, we are now interested in applying optical methods to analyse cell deposition/adhesion, in order to complete a set of experiments already performed with other techniques with Candida, Enterococcus and Staphyloccocus strains.
I am planning now to acquire such a type of instrument, and I am specially interested in
I would very much appreciate your advice on which of these two techniques have been proven to be more useful for this kind of experiments, and also advice on some good and cost-effective equipment (model).
Of course if some of you have already experience in this kind of experiments and are open to a collaboration to get more insight into these adhesion phenomena, I will be mostly grateful if you contact me to discuss it further.
Best wishes,
Antonio Méndez Vilas Interfacial Phenomena and Biosurfaces Group Department of Physics Universidad de Extremadura Avda de Elvas s/n 06001 Badajoz SPAIN E-mail: amvilas-at-unex.es
From MicroscopyL-request-at-ns.microscopy.com Sat Feb 7 22:33:37 2004
I miss that orange paint! I used to work on it when was in Germany! I hope you'll find good home for it. Sergey
At 10:39 AM 1/8/2004 -0500, you wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
From MicroscopyL-request-at-ns.microscopy.com Sun Feb 8 20:11:24 2004
The simplest IRM is with a confocal in refelction mode. We do both widefield and confocal (BioRad Radiance 2000 and Leica AOBS); the latter method is simpler even taking into account the possibility of regular reflectance muddying the interpretation.
Confocal may be fine for thick samples. With thin samples the resolution in the Z axis is insufficent to know whether you really are at the substrate.
If the substrate materials are thin, transparent, uniform and the index of refraction different enough from the sample, maybe fluorescence with TIRF could be a way to go.
} Search the CONFOCAL archive at } http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal } } Hi, } } our group is studying interaction of microbial cells (yeast and bacteria) to } several kinds of biomaterials. We conduct physico/chemical analysis of the } involved surfaces (mainly throgh surface thermodynamical analysis: contact } angles with several liquids) and have also an atomic force microscopy for } high resolution mapping of the surface topography and force measurements. } } At this moment, we are interested in seeing what is happening with the } contact side of the cell with the substratum. For this reason, we are now } interested in applying optical methods to analyse cell deposition/adhesion, } in order to complete a set of experiments already performed with other } techniques with Candida, Enterococcus and Staphyloccocus strains. } } I am planning now to acquire such a type of instrument, and I am specially } interested in } } 1. Total internal reflection microscopy } 2. Confocal Laser scanning microscopy (CLSM) } } I would very much appreciate your advice on which of these two techniques } have been proven to be more useful for this kind of experiments, and also } advice on some good and cost-effective equipment (model). } } Of course if some of you have already experience in this kind of experiments } and are open to a collaboration to get more insight into these adhesion } phenomena, I will be mostly grateful if you contact me to discuss it } further. } } Best wishes, } } Antonio Méndez Vilas } Interfacial Phenomena and Biosurfaces Group } Department of Physics } Universidad de Extremadura } Avda de Elvas s/n } 06001 Badajoz } SPAIN } E-mail: amvilas-at-unex.es } } } }
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 06:57:25 2004
Dear Colleagues, I would like to ask you for advice regarding the following problem: We are trying to section (preferentially cryo section) a highly porous collagen scaffold (sponge like) soaked with water. Problem 1: We have tried to make 8-12 µm cryo sections, but unfortunately the section always collapses. Sectioning pFA fixed collagene scaffold
was also not successful. Problem 2: Apart from this it is not possible to keep it fixed on
a slide during antibody staining procedures.
Does anybody have experience with such a “difficult” object?
Which other fixation method could be used to make the sponge stiffer to improve integrity of the sections?
Any suggestions would be helpful,
thanks in advance
Daniel.
-- (-)-(-) --------------------------- \"/ --- Dr. Daniel Eberhard =V=
about 15 years ago i did thin cross sections of cells grown on filter inserts coated with collagen. there was no end of trouble, especially at the interface of the filter and cells. after investigation i discovered that the species of collagen used, type IV, was actually quite hydrophilic. i ascribed my problems to interference with the whole processing system. no matter how hard i tried, it looked like i could not infiltrate through and across the barrier. i never did try any water based resins, though. i have durcupan around and it would have been easy to confirm.
i do not know if the hydrophilicity/hydrophobicity of the collagen type you are using could be a factor in your preparation methods, but you might want to check it out.
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-954 Cell:204-781-1502 Fax:204-789-3926
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 09:54:54 2004
You need a professional photo lab that will develop the the film the way you want it done, not with whatever film developer they have on hand. Custom black and white services might cost more than you expect, shop around. Select a few labs (if you can't find a lab go to a photo shop that caters to professionals, Jessops or Robert White, and ask them) and show them what you have and what you want. You may find it is faster, easier, cheaper and more consistant to learn to process the film yourself. Fancy equipment with nitrogen burst agitation is not necessary, fresh chemicals, film racks, several tanks and a light-tight room with a sink is all you need.
Geoff
McGovern, Gillian wrote:
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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 10:34:04 2004
You might also consider sending your negatives to an EM lab that would agree to process them for you. First, the lab should have all the proper sized holders, etc. Second, they are probably already familiar with this #$&%^$!!! film. Third, they may very well charge substantially less than a custom black and white processor would.
Just a thought.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---Nano's R'Us! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-5414 Email: tindallr-at-missouri.edu Web: http://www.biotech.missouri.edu/emc/
-----Original Message----- } From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu] Sent: Monday, February 09, 2004 1:05 PM To: McGovern, Gillian Cc: 'Microscopy-at-MSA.Microscopy.Com'
You need a professional photo lab that will develop the the film the
way you want it done, not with whatever film developer they have on hand. Custom black and white services might cost more than you expect, shop around. Select a few labs (if you can't find a lab go to a photo shop that caters to professionals, Jessops or Robert White, and ask them) and show them what you have and what you want. You may find it is faster, easier, cheaper and more consistant to learn to process the film
yourself. Fancy equipment with nitrogen burst agitation is not necessary, fresh chemicals, film racks, several tanks and a light-tight room with a sink is all you need.
Geoff
McGovern, Gillian wrote:
} ----------------------------------------------------------------------- } ------- } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 10:58:57 2004
Where: Forcheimer Building, 3rd Floor Lecture Hall Albert Einstein College of Medicine of Yeshiva University 1300 Morris Park Avenue Bronx, NY
What: A two-day "mini-workshop" with lectures, guest speakers, presentations, demonstrations and hands-on sessions for cryoultramicrotomy and associated techniques (cryo tool making, glass knife making, evaluation of glass knives, care and cleaning of diamond knives, operation of the ultramicrotome and immunoelectronmicroscopy).
Important Info: Attendance is open for all of the presentations and demonstrations on Thursday, February 26th. However, attendance is limited for the cryoultramicrotomy hands-on sessions on Friday, February 27th.
Contacts: For additional details, to RSVP or to reserve a spot for the hands-on sessions, please contact either Gloria Stephney at Albert Einstein College of Medicine ( {stephney-at-aecom.yu.edu} , 718.430.4027) or Kim Megaw at Boeckeler Instruments, Inc. ( {Kim-at-boeckeler.com} , 800.552.2262).
Sponsors and Organizers: RMC Products Group, Boeckeler Instruments, Inc., Albert Einstein College of Medicine and The New York Society of Experimental Microscopists (NYSEM).
Hope to see you there!
Bob Chiovetti Boeckeler Instruments, Inc.
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 11:55:37 2004
Sorry, but I have to disagree with Geoff about the last part of his message: with the new formulation of the 4489 film, a nitrogen burst system seems to be necessary. We have discussed problems with the 4489 film before on this listserver, and the main conclusion seemed to be that proper agitation during development was crucial to get good results. If I recall well, all the people who use appropriate agitation system (like we do in our lab) seemed to avoid the problems that have been reported with the 4489 films. When it comes to quality of development, I don't agree that we should go for the cheapest or easiest option, but instead look for reproducibility and quality.
Marc
On Monday, February 9, 2004, at 02:04 PM, Geoff McAuliffe wrote:
} } } ----------------------------------------------------------------------- } ------- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } -------- } } You need a professional photo lab that will develop the the film } the way you want it done, not with whatever film developer they have } on hand. Custom black and white services might cost more than you } expect, shop around. Select a few labs (if you can't find a lab go to } a photo shop that caters to professionals, Jessops or Robert White, } and ask them) and show them what you have and what you want. You may } find it is faster, easier, cheaper and more consistant to learn to } process the film yourself. Fancy equipment with nitrogen burst } agitation is not necessary, fresh chemicals, film racks, several tanks } and a light-tight room with a sink is all you need. } } Geoff } } McGovern, Gillian wrote: } } } ---------------------------------------------------------------------- } } -------- } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.msa.microscopy.com/MicroscopyListserver } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } --------- } } } } } } I'm having serious problems achieving adequate development of the new } } Kodak } } 4489 EM film. We have no facilities to carry out development on site } } and } } have to send film packages to another lab, subsequently, the quality } } of } } development is at the discretion of the other (non-commercial) lab. } } Basically its a hit or miss even using new development protocols. Its } } got to the stage Id be willing to send our film anywhere in the UK } } (maybe Europe) to be developed commercially if consistently good } } quality can } } be achieved. Does anyone know of somewhere which offers this service? } } } } Thanks. } } } } Veterinary Laboratories Agency (VLA) } } } } This email and any attachments is intended for the named recipient } } only. Its } } unauthorised use, disclosure, storage or copying is not permitted. If } } you have } } received it in error, please destroy all copies and inform the } } sender. Whilst this } } email and associated attachments will have been checked for known } } viruses } } whilst within VLA systems we can accept no responsibility once it has } } left our } } systems. Communications on VLA's computer systems may be monitored } } and/or recorded to secure the effective operation of the system and } } for other } } lawful purposes. } } } } } } } } } }
-- Marc Pypaert Department of Cell Biology Center for Cell and Molecular Imaging Ludwig Institute for Cancer Research Yale University School of Medicine 333 Cedar Street, PO Box 208002 New Haven, CT 06520-8002 TEL 203-785 3681 FAX 203-785 7446
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 12:28:10 2004
In my hands, nitrogen burst is not needed to get good results with the new 4489 film. I use fresh chemicals (mixed in distilled water), proper temperature, and agitation by hand. I agree that quality and consistancy is the top priority which is why I suggested she learn to do it at her facility. I know that problems with processing the new 4489 have been discussed on this forum before, I just have not had any.
Geoff
Marc Pypaert wrote:
} Sorry, but I have to disagree with Geoff about the last part of his } message: } with the new formulation of the 4489 film, a nitrogen burst system seems } to be necessary. We have discussed problems with the 4489 film before } on this listserver, and the main conclusion seemed to be that proper } agitation during development was crucial to get good results. If I } recall well, all the people who use appropriate agitation system (like } we do in our lab) seemed to avoid the problems that have been reported } with the 4489 films. When it comes to quality of development, I don't } agree } that we should go for the cheapest or easiest option, but instead look } for reproducibility and quality. } } Marc } } } On Monday, February 9, 2004, at 02:04 PM, Geoff McAuliffe wrote: } } } } } } } ----------------------------------------------------------------------- } } ------- } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.msa.microscopy.com/MicroscopyListserver } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } -------- } } } } You need a professional photo lab that will develop the the film } } the way you want it done, not with whatever film developer they have } } on hand. Custom black and white services might cost more than you } } expect, shop around. Select a few labs (if you can't find a lab go } } to a photo shop that caters to professionals, Jessops or Robert } } White, and ask them) and show them what you have and what you want. } } You may find it is faster, easier, cheaper and more consistant to } } learn to process the film yourself. Fancy equipment with nitrogen } } burst agitation is not necessary, fresh chemicals, film racks, } } several tanks and a light-tight room with a sink is all you need. } } } } Geoff } } } } McGovern, Gillian wrote: } } } } } ---------------------------------------------------------------------- } } } -------- } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.msa.microscopy.com/MicroscopyListserver } } } On-Line Help } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------- } } } --------- } } } } } } } } } I'm having serious problems achieving adequate development of the } } } new Kodak } } } 4489 EM film. We have no facilities to carry out development on } } } site and } } } have to send film packages to another lab, subsequently, the } } } quality of } } } development is at the discretion of the other (non-commercial) lab. } } } Basically its a hit or miss even using new development protocols. } } } Its got to the stage Id be willing to send our film anywhere in the UK } } } (maybe Europe) to be developed commercially if consistently good } } } quality can } } } be achieved. Does anyone know of somewhere which offers this service? } } } } } } Thanks. } } } } } } Veterinary Laboratories Agency (VLA) } } } } } } This email and any attachments is intended for the named recipient } } } only. Its } } } unauthorised use, disclosure, storage or copying is not permitted. } } } If you have } } } received it in error, please destroy all copies and inform the } } } sender. Whilst this } } } email and associated attachments will have been checked for known } } } viruses } } } whilst within VLA systems we can accept no responsibility once it } } } has left our } } } systems. Communications on VLA's computer systems may be monitored } } } and/or recorded to secure the effective operation of the system and } } } for other } } } lawful purposes. } } } } } } } } } } } } } } } } } } } -- } Marc Pypaert } Department of Cell Biology } Center for Cell and Molecular Imaging } Ludwig Institute for Cancer Research } Yale University School of Medicine } 333 Cedar Street, PO Box 208002 } New Haven, CT 06520-8002 } TEL 203-785 3681 } FAX 203-785 7446 }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 12:51:37 2004
We have been manually agitating our films since we started and have had good luck. We do a "lift-tilt rigt-tilt then tap down" about every 10 sec for the four minutes of developing to get good negatives. We also discovered (with help through the list-server) that using "Indicator Stop-Bath" caused some streaking. Once we stopped using that and started washing in running tap-water for 1 minute after developing and before "Rapid Fix", the streaking went away. My advise - make sure you agitate well - on a regular schedule. Stan
Marc Pypaert wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } Sorry, but I have to disagree with Geoff about the last part of his } message: } with the new formulation of the 4489 film, a nitrogen burst system seems } to be necessary. We have discussed problems with the 4489 film before } on this listserver, and the main conclusion seemed to be that proper } agitation during development was crucial to get good results. If I } recall well, all the people who use appropriate agitation system (like } we do in our lab) seemed to avoid the problems that have been reported } with the 4489 films. When it comes to quality of development, I don't } agree } that we should go for the cheapest or easiest option, but instead look } for reproducibility and quality. } } Marc } } On Monday, February 9, 2004, at 02:04 PM, Geoff McAuliffe wrote: } } } } } } } ----------------------------------------------------------------------- } } ------- } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.msa.microscopy.com/MicroscopyListserver } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } -------- } } } } You need a professional photo lab that will develop the the film } } the way you want it done, not with whatever film developer they have } } on hand. Custom black and white services might cost more than you } } expect, shop around. Select a few labs (if you can't find a lab go to } } a photo shop that caters to professionals, Jessops or Robert White, } } and ask them) and show them what you have and what you want. You may } } find it is faster, easier, cheaper and more consistant to learn to } } process the film yourself. Fancy equipment with nitrogen burst } } agitation is not necessary, fresh chemicals, film racks, several tanks } } and a light-tight room with a sink is all you need. } } } } Geoff } } } } McGovern, Gillian wrote: } } } } } ---------------------------------------------------------------------- } } } -------- } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.msa.microscopy.com/MicroscopyListserver } } } On-Line Help } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------- } } } --------- } } } } } } } } } I'm having serious problems achieving adequate development of the new } } } Kodak } } } 4489 EM film. We have no facilities to carry out development on site } } } and } } } have to send film packages to another lab, subsequently, the quality } } } of } } } development is at the discretion of the other (non-commercial) lab. } } } Basically its a hit or miss even using new development protocols. Its } } } got to the stage Id be willing to send our film anywhere in the UK } } } (maybe Europe) to be developed commercially if consistently good } } } quality can } } } be achieved. Does anyone know of somewhere which offers this service? } } } } } } Thanks. } } } } } } Veterinary Laboratories Agency (VLA) } } } } } } This email and any attachments is intended for the named recipient } } } only. Its } } } unauthorised use, disclosure, storage or copying is not permitted. If } } } you have } } } received it in error, please destroy all copies and inform the } } } sender. Whilst this } } } email and associated attachments will have been checked for known } } } viruses } } } whilst within VLA systems we can accept no responsibility once it has } } } left our } } } systems. Communications on VLA's computer systems may be monitored } } } and/or recorded to secure the effective operation of the system and } } } for other } } } lawful purposes. } } } } } } } } } } } } } } } } } } } -- } Marc Pypaert } Department of Cell Biology } Center for Cell and Molecular Imaging } Ludwig Institute for Cancer Research } Yale University School of Medicine } 333 Cedar Street, PO Box 208002 } New Haven, CT 06520-8002 } TEL 203-785 3681 } FAX 203-785 7446
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 14:14:22 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lee4502us-at-yahoo.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, February 9, 2004 at 11:05:16 ---------------------------------------------------------------------------
Question: Our group is currently using an Optiphot 200 C microscope that is fitted for episcopic investigation. I would like to include an external lighting source (fiber optic maybe) to enable tranmitted light microscopy. What are the issues (like lenses and apertures) I need to be mindful of in order to set up a diascopic illumination system? I look forward to your response. Thank you. Sridhar
I am curious how other software handles 12 to 8 bit conversion of data. Improvision apparently scales directly from 4096 to 255 and 0 to 0 regardless of the image data. It seems to me that you should take the maximum value in 12 bits and scale that to 255 and the minimum value and scale that to 0. Does other software do this differently, or allow you control of the conversion process? I thought that if you needed to highlight low and high values, it would be nice to be able to do this interactively in the conversion process. --
Dr. David Knecht Department of Molecular and Cell Biology University of Connecticut 91 N. Eagleville Rd. U-3125 Storrs, CT 06269-3125 knecht-at-uconn.edu 860-486-2200 860-486-4331 (fax) home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 18:31:51 2004
There are several different issues here, and hence different answers. Autoscaling the image into an 8 bit space will give you the maximum contrast, but it means that each picture will have a different relationship between the original pixel value and the final result, which would make densitometry or anything related to pixel value impossible to calibrate, so absolute conversion by simple division would be preferred in that case. Also, of course, it is much faster, taking only a single pass through the data and that just being a bit shift instead of subtraction and division.
Further, there is no good reason to restrict the scaling process to linear. You should perform any gamma correction, equalization, etc. on the full 12 bits before truncating to 8, in order to lose as little of the precision as possible.
But why do you want to go to 8 bits anyway? If you have a software package that does not handle 12 bits directly, you would do better to multiply the data up to a 16 bit range and preserve all of the information present. Most modern programs, even the newest version of Photoshop, provide pretty full capabilities for processing and measuring 16 bit per channel images.
====
In a message dated 2/9/04 6:59:35 PM, knecht-at-uconn.edu writes:
} I am curious how other software handles 12 to 8 bit conversion of } data. Improvision apparently scales directly from 4096 to 255 and 0 } to 0 regardless of the image data. It seems to me that you should } take the maximum value in 12 bits and scale that to 255 and the } minimum value and scale that to 0. Does other software do this } differently, or allow you control of the conversion process? I } thought that if you needed to highlight low and high values, it would } be nice to be able to do this interactively in the conversion process.
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 9 19:09:11 2004
DEPARTMENT OF CHEMICAL ENGINEERING AND MATERIALS SCIENCE UNIVERSITY OF CALIFORNIA-DAVIS
A postdoctoral position is available in the Electron Microscopy Facility at the University of California-Davis (UCD). This position is tied to the recent purchase of a 200kV Field-Emission JEOL 2500SE equipped with Noran EDS and Gatan Imaging Filter, which will be installed at UCD in May 2004. This microscope will support varied research programs within the Nanophases in the Environment, Agriculture and Technology (NEAT) initiative at UCD, with the successful postdoctoral candidate expected to establish collaborative research efforts with both expert and novice users of advanced electron microscopes. Successful candidates will be recent Ph.D. graduates in physics, metallurgy, or materials science with a sound background in the relevant materials issues and an ambition to be part of a developing microscopy facility. Please send a resume and publication list to Professor Nigel D. Browning at the address below. Prior experience with the many analytical and imaging methods used in both STEM and TEM is essential, and experience with the expectations of multi-user facilities in the US is desirable. The position is for one year initially, with possibilities existing for further years. It is also expected that UCD will hire a full-time staff member to perform similar duties in the very near future. Salary is commensurate with experience.
Nigel D. Browning
Department of Chemical Engineering and Materials Science University of California-Davis One Shields Ave Davis, CA 95616
AND
National Center for Electron Microscopy, MS 72-150 Lawrence Berkeley National Laboratory Berkeley, CA 94720
DEPARTMENT OF CHEMICAL ENGINEERING AND MATERIALS SCIENCE UNIVERSITY OF CALIFORNIA-DAVIS
A postdoctoral position is available in the Interface Physics Group at the University of California-Davis (UCD) to work on novel developments in ultrafast TEM. This position is linked to the major new program being initiated at Lawrence Livermore National Laboratory (LLNL) involving the development of a new dynamic TEM (DTEM) to study materials with a time resolution in the nano- to femto-second regime. This position is intended to initially spend 6-9 months in Berlin working on the existing state-of-the-art instrument at TU-Berlin, before transferring back to UCD/LLNL to work on a new instrument that will be installed at LLNL. In addition to becoming expert in the use of the DTEM, the aim of the first years work is to use the microscope to study phase transformations. Successful candidates will be recent Ph.D. graduates in physics, metallurgy, or materials science with a sound background in the relevant materials issues and an ambition to be part of a developing program pushing at the frontiers of interface science. Please send a resume and publication list to Professor Nigel D. Browning at the address below. Prior experience in STEM or TEM is essential. However, consideration will be based on the candidates overall potential for success in the field and applicants with prior experience in related fields are encouraged to apply. The position is for one year initially, with possibilities existing for further years. In addition, the dynamic TEM project is expected to become a major new program at LLNL, offering the strong possibility of a permanent staff position in the future. Salary is commensurate with experience.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (alvarobq-at-fcien.edu.uy) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Tuesday, February 10, 2004 at 12:29:00 ---------------------------------------------------------------------------
Email: alvarobq-at-fcien.edu.uy Name: alvaro d. olivera
Organization: sciences university
Education: Graduate College
Location: montevideo, montevideo, uruguay
Question: I¥m cutting 61 nm sections of rat nerve tissue perfussion fixed and post fixed by immersion in PAF 4% Glutaraldehyde 0,25% Picric acid 15%. embbedig media: LRWhite (SPI supplies) medium grade just for use( include benzoyl peroxide) whithout accelerator. mounting: 300 mesh Ni grids whithout film, covered with Coat-Grid Pen. buffer: PBS 0,1M + BSA 0,1% + Triton x-100 0,05% The problem is after the immunoreaction whith gold conjugate antibody...the sections looks too much dry under the magnifying glass and 80% of sections disappear. Even when we put the grid into the T.E.M. the image is horrible, we can¥t recognize nothing and we see a rare artifact what i think it¥s a tissue rests stick over the grid. staining: uranium acetate 2% water(milli-q)diluted. Please, I need a solution for this problem. hope for your reply, many thanks...Alvaro. ATTN: the same tissue mounted over Cu grids and stained whit uranium acetate and Pb citrate, NO immunoreaction!!!, are great in T.E.M.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (guffey1-at-inter-linc.net) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, February 10, 2004 at 12:43:10 ---------------------------------------------------------------------------
Email: guffey1-at-inter-linc.net Name: Jennifer Guffey
Organization: southwest missouri state university
Education: Undergraduate College
Location: springfield, missouri, USA
Question: why is the image dimmer when you switch from a low power to a high power objective?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (zeriena-at-yahoo.com) from http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, February 10, 2004 at 12:20:12 ---------------------------------------------------------------------------
Email: zeriena-at-yahoo.com Name: rose zareen george
Organization: quens college
Education: Undergraduate College
Location: flushing, NY,USA
Question: 1. in the objective 4x, what does the number .12 indicate(numerical Aparature) (eg. for 10x this number is .25 and 20 it is .50 and so on.. 2.how many micro meter per objective is 43x..
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mparthiban-at-rediffmail.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, February 10, 2004 at 12:41:32 ---------------------------------------------------------------------------
Question: I need to send some pathogenic bacterial samples for SEM. So I need to kill the bacteria with either formaldehyde or gluteraldehyde before sending. Can any one say the concentration I should use or where can i find the prodedure for sending bacterial samples for electron microscopy. Parthi
When you use uncoated grids the first thing to change is the thickness of your sections. Cut the sections so they are light-medium gold in color. Your 61nm sections are too delicate and therefore when you do all of those different incubations and washes they break(just staining them with UA and lead doesn't have the same effect). Remember the LR White sections are extremely hydrophilic and swell up when placed into any aqueous solution.
If you can, try using formvar coated nickel grids. The sections will stay on and won't disappear or loosen with the formvar. I always use 200 mesh formvar coated nickel grids for all my ImmunoEM labeling procedures.
One more thing is to be very careful not to let the grids become dry at any time during the entire labeling procedure.
Good Luck!
Karen Bentley Karen L. Bentley, M.S.(previously Jensen) Associate Scientist & Project Manager Electron Microscope Research Core University of Rochester Medical Center Rochester, NY 14642 585-275-1954
-----Original Message----- } From: alvarobq-at-fcien.edu.uy [mailto:alvarobq-at-fcien.edu.uy] Sent: Tuesday, February 10, 2004 3:28 PM To: MicroscopyListserver
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (alvarobq-at-fcien.edu.uy) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Tuesday, February 10, 2004 at 12:29:00 ---------------------------------------------------------------------------
Email: alvarobq-at-fcien.edu.uy Name: alvaro d. olivera
Organization: sciences university
Education: Graduate College
Location: montevideo, montevideo, uruguay
Question: I¥m cutting 61 nm sections of rat nerve tissue perfussion fixed and post fixed by immersion in PAF 4% Glutaraldehyde 0,25% Picric acid 15%. embbedig media: LRWhite (SPI supplies) medium grade just for use( include benzoyl peroxide) whithout accelerator. mounting: 300 mesh Ni grids whithout film, covered with Coat-Grid Pen. buffer: PBS 0,1M + BSA 0,1% + Triton x-100 0,05% The problem is after the immunoreaction whith gold conjugate antibody...the sections looks too much dry under the magnifying glass and 80% of sections disappear. Even when we put the grid into the T.E.M. the image is horrible, we can¥t recognize nothing and we see a rare artifact what i think it¥s a tissue rests stick over the grid. staining: uranium acetate 2% water(milli-q)diluted. Please, I need a solution for this problem. hope for your reply, many thanks...Alvaro. ATTN: the same tissue mounted over Cu grids and stained whit uranium acetate and Pb citrate, NO immunoreaction!!!, are great in T.E.M.
Karen L. Bentley, M.S.(previously Jensen) Associate Scientist & Project Manager Electron Microscope Research Core University of Rochester Medical Center Rochester, NY 14642 585-275-1954
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 10 18:26:22 2004
I can't speak for other software, but here is how we do this in analySIS:
Let me first make the observation, that (to my knowledge) there are no monitors that can actually display more than 8 bit of gray levels. This alone makes a bit reduction necessary for display purposes. When we open an image that is more than 8 bit (12-bit or 16-bit, for example), the software must calculate new values for the display which fall between 0 and 255. We typically do that by matching the highest pixel value with 255, the lowest to 0. There are some finer details that have to do with hot and dead pixels, but I won't go into that. This automatically results in a contrast maximized image on the screen. At this point you have several options: 1) You can manipulate the underlying image data, which are still 12 bit, and keep the way the data is displayed, or 2) you keep the underlying data and change the way they are displayed. Both have methods have their justifications. When it comes to actually create an 8-bit image from the 12-bit original, you only need to "read" the display data (which are 8-bit), and create a new image. Done.
The question remains: should you do this conversion? In general, it is better to keep the 12-bit image until the end of a process, and only convert it to 8-bit if really necessary. There is definitely data loss during this process, be it through rounding errors or range compression. My personal rule of thumb: If you need to further analyze the image, don't change the bit depth. If the image is only used for display (such as in a paper or on the web), reducing the bit depth is probably not a big problem.
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: David Knecht [mailto:knecht-at-uconn.edu] Sent: Monday, February 09, 2004 16:02 To: microscopy-at-sparc5.microscopy.com
I am curious how other software handles 12 to 8 bit conversion of data. Improvision apparently scales directly from 4096 to 255 and 0 to 0 regardless of the image data. It seems to me that you should take the maximum value in 12 bits and scale that to 255 and the minimum value and scale that to 0. Does other software do this differently, or allow you control of the conversion process? I thought that if you needed to highlight low and high values, it would be nice to be able to do this interactively in the conversion process. --
Dr. David Knecht Department of Molecular and Cell Biology University of Connecticut 91 N. Eagleville Rd. U-3125 Storrs, CT 06269-3125 knecht-at-uconn.edu 860-486-2200 860-486-4331 (fax) home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 11 12:23:29 2004
I have just inherited an old LKB 2078 Histo Knifemaker. Not the instruction book, just the machine. It looks similar to a 7800, but very different at the same time.
Does anyone have a copy of the instruction book they can let me copy? Has anyone used one of these for making 45 degree knives with 10mm glass? Does this thing handle just like a 7800?
If you know anything about this 2078 please let me know.
On the "cutting" edge of science,
Paula :-)
Paula Sicurello George Washington Univ. Medical Center Electron Microscope Lab Washington, DC 20037 202-994-2930 phone 202-994-2518 fax
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 11 13:30:41 2004
In a message dated 2/11/2004 1:31:14 PM Eastern Standard Time, patpxs-at-gwumc.edu writes:
} I have just inherited an old LKB 2078 Histo Knifemaker. Not the instruction book, just the machine. It looks similar to a 7800, but very different at the same time. } } Does anyone have a copy of the instruction book they can let me copy? Has anyone used one of these for making 45 degree knives with 10mm glass? Does this thing handle just like a } 7800? } } If you know anything about this 2078 please let me know. } } On the "cutting" edge of science, } } Paula :-)
Hi Paula,
Sorry, I can't help on the manual. Maybe someone else can?
Unless I'm mistaken, the 2078 was used to make Ralph knives from rectangles (thus it was called the "2078 Histo" knifemaker).
The stage may not be set up for triangular knives, but I suppose you could get creative and make some homemade guides that might make triangular knives.
As far as glass thickness, most of the LKB vintage instruments had trouble with glass that was thicker than about 6 - 8mm. Don't know if it's going to have enough "oomph" to break 10mm glass. I'd suspect not, though.
Hope the above info isn't completely useless!
Bob Chiovetti
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 11 14:52:50 2004
I have the directions for this instrument and will mail a xerox copy to you.
You should have it in a couple days.
JB
} I have just inherited an old LKB 2078 Histo Knifemaker. Not the } instruction book, just the machine. It looks similar to a 7800, but } very different at the same time. } } Does anyone have a copy of the instruction book they can let me } copy? Has anyone used one of these for making 45 degree knives with } 10mm glass? Does this thing handle just like a 7800? } } If you know anything about this 2078 please let me know. } } On the "cutting" edge of science, } } Paula :-) } } Paula Sicurello } George Washington Univ. Medical Center } Electron Microscope Lab } Washington, DC 20037 } 202-994-2930 phone } 202-994-2518 fax
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu ##############################################################
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 11 15:18:58 2004
Jennifer, Assuming that the light source intensity is held constant, this is one way to look at it.
1.) At the lower mag you are accepting light from a given circle (the field of view) and then projecting that light onto a much larger circle (your retina, or a piece of film). The intensity is reduced by the ratio of the areas of the 2 circles.
2.) At the higher mag you are accepting light from a smaller circle but projecting it onto the same size circle as before, hence the further reduction in brightness at higher magnifications.
In terms of conservation of energy (assuming no losses due to the optics, and that the aperture is the same size) the amount of light available from the field of view is equal to the area x intensity. That same amount of light is then spread over a larger area to give you your magnified image. The intensity at that circle is the total light/area. As your magnification increases, the area illuminated decreases in an inverse square relationship, i.e. if you double your magnification you will have 1/4 the area and therefore 1/4 the total light available to spread over your retina or film. That is why things look darker, or need stronger illumination, at higher magnifications.
Ken Converse owner Quality Images third party SEM service Delta, PA
by way of MicroscopyListServer wrote:
} } } ------------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (guffey1-at-inter-linc.net) from } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, } February 10, 2004 at 12:43:10 } --------------------------------------------------------------------------- } } } Email: guffey1-at-inter-linc.net } Name: Jennifer Guffey } } Organization: southwest missouri state university } } Education: Undergraduate College } } Location: springfield, missouri, USA } } Question: why is the image dimmer when you switch from a low power to } a high power objective? } } --------------------------------------------------------------------------- } } }
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 11 15:25:56 2004
They may seem a bit complicated, but that's the way light microscopes work! Best is if you can put a graticule on the specimen stage; this graticule might be marked in divisions of 10 micrometers from 0 to 1 millimeter, and then compare that with your image in some way. If you're not taking photographs, one way is to put in an eyepiece with its own graticule inside, and then count how many divisions in the eyepiece graticule correspond to, say, 0.1 mm in the specimen graticule.
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
} From: zeriena-at-yahoo.com (by way of MicroscopyListServer) } To: MicroscopyListserver {microscopy-at-msa.microscopy.com} } Subject: [Microscopy] AskAMicroscopist: LM question about Lenses } Date: Wed, 11 Feb 2004 07:27:37 +1100 } } } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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From MicroscopyL-request-at-ns.microscopy.com Wed Feb 11 16:02:21 2004
For years, I have been using Kimble "Opticlear" 5 dram vials with a *closed bottom* poly stopper to store my 12.5mm pin-style SEM (Etec) stubs/specimens. The pin can be pushed into the bottom of the closure, making the stopper hold the stub. Works well - More or less airtight, cheap, and suspends the specimen out of harm's way. I still use the pin stubs with an adapter for my Hitachi SEM. I also use a lot of similar stubs with a 25.4mm diameter.
I have never found a similar vial (closed bottom poly stopper) that is large enough to accept my 25.4mm stubs. I am aware of commercially available plastic boxes (w/inserts), but they are very expensive for what you get and do little to protect specimens.
Any ideas about where to find large vials similar to the small ones (with closed bottom cap)?
Thanks, Woody
Woody White BWXT Services: http://www.bwxt.com/bwxt.html My Site: http://woody.white.home.att.net
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 11 16:23:05 2004
I have an older Leitz Pol microscope that has served me well for many years. I've taken thousands of photographs of mineral thin sections in polarized light. Recently I fitted the scope with a Coolpix digital camera and noticed a "spot" in the center of the image that was lighter and had a greenish cast. After virtually disassembling the scope looking for the source of the problem, I finally discovered that it is only present when I cross the polars (Nicol prisms). Examination of the prisms shows a faint pale colored spot in the center of both the substage polarizer and the swing-in analyzer. I never detected this problem with film, but it is decidedly evident with the digital camera. I can remove and replace the polarizer, but still have the spot because of the analyzer. Can anyone suggest a retrofit for the analyzer using modern polaroid or some other technique? I love the Nicols, but can't live with the spot.
Henry Barwood Associate Professor of Science, Earth Science Department of Math and Physics MSCX 312G Troy State University Troy, Alabama 36082 hbarwood-at-troyst.edu
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 11 16:25:21 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cmeyer911-at-yahoo.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, February 10, 2004 at 17:51:43 ---------------------------------------------------------------------------
Email: cmeyer911-at-yahoo.com Name: Chris Meyer
Organization: Boeing
Title-Subject: [Microscopy] [Filtered] TEM Electrolyte Procedure Requested
Question: Hello,
I'm looking for a titanium electropolish procedure that does not include perchloric acid. I have perchloric, and a procedure for it, but I'm looking for alternatives for safety reasons.
The alloy is Ti-5Al-5V-5Mo-3Cr and will have alpha and beta phase. I'm looking for electrolyte recipie, temperature and voltage.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (iand-at-clemson.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, February 10, 2004 at 15:49:23 ---------------------------------------------------------------------------
Question: Hi I would like to trace the general pathway from blood vessels through the follicle cells and in to the oocyte, though various stages in oogenesis. I am not looking for any specific protien/molecule, I just want to prove/show that something travels through the follicle cell, across the zona pellucida into the egg. it will need to cross at least one membrane,(into the follicle cell.
I would like to use fluorescence or TEM. Any suggestions please. thanks ian
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ldemp-at-mse.ufl.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 11, 2004 at 08:12:08 ---------------------------------------------------------------------------
Question: Dear all: The Latin American School of Electron Microscopy (LASEM), sponsored by the Committee of Inter American Societies for Electron Microscopy (CIASEM), will be offering a 3-day workshop at the University of Central Florida in Orlando from March 10 to March 12. This workshop is part of the 2004 Joint Symposium of the Florida Chapter of the AVS Science and Technology Society (FLAVS), the Florida Society for Microscopy (FSM) and the Florida Section of the American Ceramic Society (FL-ACerS). The 3-day workshop includes fundamentals and applications of Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and associated techniques. A certificate of attendance will be given to all participants. For more information and registration please visit the symposium web-page at www.flavs.org and go to the LASEM workshop link (www.flavs.org/LASEM.htm).
Are all Pseudostratified columnar epithelial cells ciliated? I think so but I don't want to say so based on a gut feeling. Does anyone know of a pseudostratified epithelium that is not ciliated?
The converse is not true (i.e., not all cilia are on pseudostratifed epithelium since the oviduct has cilia on its simple columnar epithelium).
Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 3 Tucker Hall University of Missouri Columbia, MO 65211-7400
Hmmm...... sort of a ...... (searching for the right word here and not finding it) .... question. Since the oocyte does not exist in a vacuum it must be nourished somehow. How else if not via the follicular cells? I think you need to be more specific. Do you want to show that only certain things go into oocytes? Is this some sort of class project (design an experiment to demonstrate xyz) or ? TEM and fluorescence are very different tools with very different capabilities, pick one.
Geoff
by way of MicroscopyListServer wrote:
} --------------------------------------------------------------------------- } } } Email: iand-at-clemson.edu } Name: ian davenport } } Organization: clemson university } } Title-Subject: [Microscopy] [Filtered] MListserver: } } Question: Hi } I would like to trace the general pathway from blood vessels through } the follicle cells and in to the oocyte, though various stages in } oogenesis. I am not looking for any specific protien/molecule, I just } want to prove/show that something travels through the follicle cell, } across the zona pellucida into the egg. it will need to cross at least } one membrane,(into the follicle cell. } } I would like to use fluorescence or TEM. } Any suggestions please. } thanks } ian } } --------------------------------------------------------------------------- } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 12 10:52:45 2004
We not sure from your data that this is an application for Mercox, but it just might be. I'll refer you to a paper by Dr. Fred Hossler at East Tennessee who's got great experience with Mercox. Read the paper and see if it is what you are looking for. See http://www.laddresearch.com/Key_Products/Mercox/HosslerPaper/hosslerpaper.ht ml
John Arnott
Ladd Research 83 Holly Court Williston, VT 05495
On-line Catalog: http://www.laddresearch.com
tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US) fax: 1-802-660-8859 e-mail: sales-at-laddresearch.com ----- Original Message ----- } From: "by way of MicroscopyListServer" {iand-at-clemson.edu} To: "MicroscopyListserver" {microscopy-at-msa.microscopy.com} Sent: Wednesday, February 11, 2004 5:23 PM
Hi Tom:
I would say yes, all pseudostratified cloumnar epithelia are ciliated. By that I mean each cell has at least one cilium. I have seen (via TEM) cilia on many types of epithelia (simple cuboidal and simple columnar) that are not normally thought of as ciliated. I read something recently (don't remember where) that the cilium is a polarity signal for cell orientation and cell division.
Geoff
Tom Phillips wrote:
} This comes up every year in my histology class: } } Are all Pseudostratified columnar epithelial cells ciliated? I think } so but I don't want to say so based on a gut feeling. Does anyone } know of a pseudostratified epithelium that is not ciliated? } } The converse is not true (i.e., not all cilia are on pseudostratifed } epithelium since the oviduct has cilia on its simple columnar } epithelium). } } Thanks, Tom } } Thomas E. Phillips, PhD } Professor of Biological Sciences } Director, Molecular Cytology Core } 3 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } } 573-882-4712 (office) } 573-882-0123 (fax) } PhillipsT-at-missouri.edu } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 12 13:13:14 2004
I have a couple questions regarding the illumination stability of mercury burners:
1- When a new burner is installed, is there a period of time (within a few hours) where the intensity increases to a plateau? With an older lamp, everytime we turn the lamp on, we have observed that it takes about 40 minutes to reach a plateau. We installed a new burner recently, and images have been brighter from day to day for the first 3 days and remained stable afterwards. Is it just a coincidence?
2- To solve the stability problems we have with the mercury burners, we’re considering the use of xenon lamps. Is there somewhere a comparative stability study of the 2 types of burners?
Thank you.
Marie-Claude Belanger
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From MicroscopyL-request-at-ns.microscopy.com Thu Feb 12 14:07:28 2004
Since I will be giving the histology lecture on the male reproductive system to medical students in a couple of weeks, I should mention that one of the classic pseudostratified columnar epithelia, that of the epididymis, has abundant long microvilli ("stereocilia" to the old classical microscopists) at the apical surface but, to my knowledge, no cilia. Of course, I can't exclude the possibility that a single cilium is hidden away somewhere among all those microvilli. --Kent
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A. Kent Christensen, Professor Emeritus Department of Cell and Developmental Biology, Medical Science II Building University of Michigan Medical School, Ann Arbor, MI 48109-0616 Tel (work) (734) 763-1287, Fax (work) (734) 763-1166 akc-at-umich.edu http://www.umich.edu/~akc/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Hi Tom: } } I would say yes, all pseudostratified cloumnar epithelia are } ciliated. By that I mean each cell has at least one cilium. I have seen } (via TEM) cilia on many types of epithelia (simple cuboidal and simple } columnar) that are not normally thought of as ciliated. I read something } recently (don't remember where) that the cilium is a polarity signal for } cell orientation and cell division. } } Geoff } } Tom Phillips wrote: } } } This comes up every year in my histology class: } } } } Are all Pseudostratified columnar epithelial cells ciliated? I think } } so but I don't want to say so based on a gut feeling. Does anyone } } know of a pseudostratified epithelium that is not ciliated? } } } } The converse is not true (i.e., not all cilia are on pseudostratifed } } epithelium since the oviduct has cilia on its simple columnar } } epithelium). } } } } Thanks, Tom } } } } Thomas E. Phillips, PhD } } Professor of Biological Sciences } } Director, Molecular Cytology Core } } 3 Tucker Hall } } University of Missouri } } Columbia, MO 65211-7400 } } } } 573-882-4712 (office) } } 573-882-0123 (fax) } } PhillipsT-at-missouri.edu } } } ********************************************** } Geoff McAuliffe, Ph.D. } Neuroscience and Cell Biology } Robert Wood Johnson Medical School } 675 Hoes Lane, Piscataway, NJ 08854 } voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu } **********************************************
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 12 14:45:12 2004
} Dear Tom and Geoff, Tom's referring, I think, to the primary cilium, see- http://www.bowserlab.org/primarycilia/ciliumpage2.htm or http://www.primary-cilium.co.uk/ - this is web site relating to the primary cilium (called by some the 'forgotten organelle'), which is found, as I recently learned, in most mammalian cells.
Regards,
Richard
} Hi Tom: } } I would say yes, all pseudostratified cloumnar epithelia are } ciliated. By that I mean each cell has at least one cilium. I have } seen (via TEM) cilia on many types of epithelia (simple cuboidal and } simple columnar) that are not normally thought of as ciliated. I read } something recently (don't remember where) that the cilium is a } polarity signal for cell orientation and cell division. } } Geoff } } Tom Phillips wrote: } } } This comes up every year in my histology class: } } } } Are all Pseudostratified columnar epithelial cells ciliated? I think } } so but I don't want to say so based on a gut feeling. Does anyone } } know of a pseudostratified epithelium that is not ciliated? } } } } The converse is not true (i.e., not all cilia are on pseudostratifed } } epithelium since the oviduct has cilia on its simple columnar } } epithelium). } } } } Thanks, Tom } } } } Thomas E. Phillips, PhD } } Professor of Biological Sciences } } Director, Molecular Cytology Core } } 3 Tucker Hall } } University of Missouri } } Columbia, MO 65211-7400 } } } } 573-882-4712 (office) } } 573-882-0123 (fax) } } PhillipsT-at-missouri.edu } } } } } } } } -- } -- } ********************************************** } Geoff McAuliffe, Ph.D. } Neuroscience and Cell Biology } Robert Wood Johnson Medical School } 675 Hoes Lane, Piscataway, NJ 08854 } voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu } ********************************************** } } } } } Richard Easingwood Otago Centre for Electron Microscopy c/- Department of Anatomy & Structural Biology University of Otago ph: 0064 3 479 7301 fax: 0064 3 479 7254 cell: 021 222 4759
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 13 06:13:03 2004
The intensity you see through different objective lenses does not only depend on the magnification of the lens, but also its numerical aperture, which, roughly speaking, is how wide is the cone of rays entering the lens. Here are some values for a set of polarizing lenses we use:
If everything is set up correctly, then the intensity will be roughly proportional to (n.a./mag)squared as in the last column. Generally, this factor will decrease a bit with increasing lens power, but note that our 25x lens is a bit better than the one above and below. This is because it is a high quality Zeiss Neofluar lens.
However, these factors will only apply if you have a condenser which delivers an even intensity of rays over the whole numerical aperture. For example, the 0.63 n.a. condenser we normally use will not deliver a full cone to the 40x lens, so things do appear noticeably dimmer in that one.
I'm don't know what kind of condenser you are using. One thing you can do is to dim your light source somewhat, and then take out the eyepiece and look down the tube. You will see a circle of light corresponding to the cone of rays going through your lenses. If this is cut down by an aperture, it may be that your higher power lenses are not getting their full ration in terms of incoming cone of rays. Then things will appear noticeably dimmer, and moreover you will not be getting the full resolution that your objective lens is capable of.
One other thing if you are doing polarized light microscopy. If you have a wide cone of rays coming through, say, a mineral rock section, then the colour transmitted through the polarizing system will be different for different path lengths through the specimen between the centre and outside of the cone. This is why polarizing colours tend to look much more wishy-washy through a high-power lens.
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
} From: guffey1-at-inter-linc.net (by way of MicroscopyListServer) } To: MicroscopyListserver {microscopy-at-msa.microscopy.com} } Subject: [Microscopy] [Filtered] AskAMicroscopist: Imaging question } Date: Wed, 11 Feb 2004 07:29:07 +1100 } } } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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From MicroscopyL-request-at-ns.microscopy.com Fri Feb 13 06:46:27 2004
Thanks to everyone who replied with their suggestions to prevent image retrieval failures.
There was one particular procedure which we were unaware of and most Windows users do not seem to know this control feature. I would like to pass this along to the group.
While the compact flash cards are typically read by USB devices and in theory, are swappable, we have not had failures since software "ejecting" the card. Some card readers require physically ejecting the card much like the camera card eject. We simply pull the cards out of the card readers. Apparently, Windows does not always know that the card has been removed and that the files have changed, hence the files are corrupted and not readable. The solution (hopefully in the long run) is go open My Computer, right click on the card reader device and eject the card. While I recalled seeing this previously, I did not think it was relevant. We never had this problem with our USB jump drives.
I hope this helps everyone else who had similar problems.
Alan Stone ASTON Metallurgical Services
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 13 08:01:34 2004
So far as I know, the epithelium of the epididymus is pseudostratified, with STEROCILIA (which are actually elongate microvilli). I do not recall any descriptions - on it - of cilia, primary or otherwise. MRA
Mark R. Adelman, Ph.D. Associate Professor of Anatomy, Physiology & Genetics Uniformed Services University of the Health Sciences 4301 Jones Bridge Road Bethesda, MD 20814-4799 USA Phone: 301-295-3208 FAX: 301-295-1786 Email: madelman-at-usuhs.mil Website: http://bicmra.usuhs.mil/ On Thursday, February 12, 2004, at 04:23 PM, Geoff McAuliffe wrote:
} } } ----------------------------------------------------------------------- } ------- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } -------- } } Hi Tom: } } I would say yes, all pseudostratified cloumnar epithelia are } ciliated. By that I mean each cell has at least one cilium. I have } seen (via TEM) cilia on many types of epithelia (simple cuboidal and } simple columnar) that are not normally thought of as ciliated. I read } something recently (don't remember where) that the cilium is a } polarity signal for cell orientation and cell division. } } Geoff } } Tom Phillips wrote: } } } This comes up every year in my histology class: } } } } Are all Pseudostratified columnar epithelial cells ciliated? I think } } so but I don't want to say so based on a gut feeling. Does anyone } } know of a pseudostratified epithelium that is not ciliated? } } } } The converse is not true (i.e., not all cilia are on pseudostratifed } } epithelium since the oviduct has cilia on its simple columnar } } epithelium). } } } } Thanks, Tom } } } } Thomas E. Phillips, PhD } } Professor of Biological Sciences } } Director, Molecular Cytology Core } } 3 Tucker Hall } } University of Missouri } } Columbia, MO 65211-7400 } } } } 573-882-4712 (office) } } 573-882-0123 (fax) } } PhillipsT-at-missouri.edu } } } } } } } } -- } -- } ********************************************** } Geoff McAuliffe, Ph.D. } Neuroscience and Cell Biology } Robert Wood Johnson Medical School } 675 Hoes Lane, Piscataway, NJ 08854 } voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu } ********************************************** } } } } }
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 13 09:30:36 2004
I am contemplating attempting to assemble a UV fluorescence microscope. I want Ex ~ 305 nm and Em ~350 to 400. I have found the necessary filters and plan to assemble a set for ($800+), we have a quartz objective and UV lamp and camera. My questions are... Is anyone imaging tryptophan fluorescence so I can go there and try my idea before I spend a lot of money, or has anyone ever tried to do this and can offer words of wisdom or caution.
thanks, heather
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 13 12:40:25 2004
Hello, I have a request from a science museum to take a few SEM photos of a microproceessor from a cell phone. I was wondering if anyone has any recommendations on methods to remove the packaging material to get to the actual silicon.
It looks like a Texas instruments chip with number MAD2WD1, if that helps. Any comments appreciated. Gordon
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ Gordon Ante Vrdoljak Electron Microscope Lab ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall gvrdolja-at-nature.berkeley.edu UC Berkeley phone (510) 642-2085 Berkeley CA 94720-3330 fax (510) 643-6207 cell (510) 290-6793
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 13 13:02:19 2004
I can give you very specific information on that. However, I must caution you that this procedure should only be performed in a fume hood, with full protective clothing, eyewear and someone with training in doing this..
The mold compound may be dissolved in a either red fuming nitric acid heated to +80C or a combination of 90% nitric/ 10% sulfuric. The process is by immersion. Bear in mind that this is a very hazardous procedure, so now that I told you some specifics, let me make an alternate suggestion. Simply take a "ceramic" device, one that has a soldered lid on it and grind the lid off. This way you won't have to deal with any of this dangerous material and I assure you that unless your audience are semiconductor engineers, they won't know one circuit from the next.
Let me know if there is something specific about this or if you just want to show the general construction of an IC. I have some archived photos I'd be happy to pass along if you'd simply need something as an example.
Peter Tomic Agere Systems Allentown, PA
-----Original Message----- } From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU] Sent: Friday, February 13, 2004 1:51 PM To: Microscopy-at-MSA.Microscopy.Com
Hello, I have a request from a science museum to take a few SEM photos of a microproceessor from a cell phone. I was wondering if anyone has any recommendations on methods to remove the packaging material to get to the actual silicon.
It looks like a Texas instruments chip with number MAD2WD1, if that helps. Any comments appreciated. Gordon
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ Gordon Ante Vrdoljak Electron Microscope Lab ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall gvrdolja-at-nature.berkeley.edu UC Berkeley phone (510) 642-2085 Berkeley CA 94720-3330 fax (510) 643-6207 cell (510) 290-6793
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 13 17:10:49 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sue.tyler-at-noaa.gov) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, February 13, 2004 at 13:30:11 ---------------------------------------------------------------------------
Email: sue.tyler-at-noaa.gov Name: Sue Tyler
Organization: Cooperative Oxford Laboratory
Title-Subject: [Microscopy] [Filtered] MListserver: neutralization of osmium tetroxide
Question: I recently submitted a container of neutralized (2% Osmium in 20ml of corn oil)Osmium Tetroxide to our waste disposal and safety person and it was rejected. The reason for the rejection was that we are not licensed to treat our waste in this facility. Has anyone else run into this problem? I would think that it would be safer to have it neutralized in case of a spill etc. Any opinions would be nice.
Reference: Cooper, K. (1988) Neutralization of Osmium Tetroxide in case of accidental spillage and for disposal. Bulletin of The Microscopical Soxiety of Canada. 8:24-28
we also are not allowed to treat our used materials (we aren't allowed to call it waste). we can do something as part of an approved protocol. maybe you need to "test" your osmium is reactive afterwards by putting it in corn oil and see if it turns black! that makes it part of the experiment and not part of the disposal. see if that is an approved reason for doing what you do.
At 07:14 AM 2/14/2004 +0800, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 3 Tucker Hall University of Missouri Columbia, MO 65211-7400
A postdoctoral position is available to work on problems related to surface reconstructions, charge density and electron crystallography. A strong background in electron microscopy is necessary. Additional experience in UHV equipment, and strong computer skills and expertise in density functional methods would be positive points.
----------------------------------------------- Laurence Marks Department of Materials Science and Engineering Northwestern University Evanston, IL 60201, USA Tel: (847) 491-3996 Fax: (847) 491-7820 mailto:ldm2-at-risc4.numis.nwu.edu http://www.numis.nwu.edu -----------------------------------------------
From MicroscopyL-request-at-ns.microscopy.com Sun Feb 15 16:39:53 2004
Yes, the number after the magnification is the magnification is the Numerical Aperture or NA.
Three quick equations which you might find helpful.
1. NA = n sin a where n = refractive index of the immersing liquid between the top of the sample and the front element of the lens a = 1/2 of the collecting angle of the objective
From this equation, you can quickly see that, the larger the collecting angle, the greater the NA. Also, the refractive index of air is 1.00, so using immersing fluids like water (1.33), glycerin (1.47), or immersion oil (1.5212) has tremendous effect on improving the NA. Reminder: use only the fluid for which the lens is engineered. Trying to use immersion oil with a "dry" (air) objective, is a disaster. Lenses built for immersing liquids are specially sealed so that the liquid does not creep into the internal construction of the lenses.
2. Why is NA important? The reasons are derived from Diffraction Theory. Every object produces a diffraction pattern (see Young's slit experiments in any physics book for details). For simple objects like stage micrometers and diatoms like pleurasigma angulatum, you can see the diffraction pattern by removing the eyepiece and peering deep down the tube into the Back Focal Plane of the objective. (You also need to close down the condernser as far as it will go, to make the source of light a very small opening or, alternatively, remove it and put a pinhole over the light port in the base of your microscope). If the NA is big enough to capture 2 adjacent spots from the diffraction pattern, enough information is present to determine spacing in the original object. The basic equation for resolution summarizes:
Rxy = (1.22x wavelength)/ (NAobjective+NAcondenser). R = the size (in the XY plane, typically in micrometers) of the smallest resolvable particle 1.22 = a shape function (assuming round apertures in the microscope) Wavelength = you can use 500 nm or 0.500 micrometers as sort of the center of the visible spectrum NA = real working NAs (note: just because a lens is engraved with a value, does not mean that that is the working NA. Closing down an iris in the back focal plane of the objective or closing down the condenser aperture iris reduces the real working NA).
If the NA is big enough to capture THREE adjacent diffraction spots (the set of -1/0/+1 only counts as 2), you will begin to get better edge definition. So, the bigger the NA, the smaller the particle that you can resolve and the better the edge quality will be.
3. As for your other question: micrometers Are you referring to the working distance (distance from the front of the lense to the top of the sample) or Field of View? If working distance: you can either measure that directly or can look up the specs in the literature from the manufacturer. If Field of view (the diameter of the territory you see when looking through the eyepieces): F. O. V = (field number)/Mobjective The field number is the value written on the EYEPIECE, following the magnification. It refers to the distance across a small ridge or baffle typically mounted about 1/3 of the way up the inside wall from the BOTTOM of the eyepiece and typically ranges from about 18mm to 25 mm (old Reichert Polyvars and some others had ultra wide field of view and went to 32mm). Convert to micrometers by multiplying by 1000. Mobjective is the Magnification of the OBJECTIVE multiplied by any other auxiliary lenses between the objective and the bottom of the eyepiece. Ex: If you were working with eyepieces which had field number 22 (22 mm or 22,000 micrometers) and a 10x objective, the size of the field of view is simply 22,000/10 or 2,000micrometers.
Note: the eyepiece going to the camera is often a different magnification than the eyepieces you look through and the chip in the camera has its own equivalent of a "magnification", so what you see in the eyepieces will often be a larger FOV than you can capture with your camera.
You might find my book, "Optimizing Light Microscopy", helpful. A description and ordering information are on our website: www.MicroscopyEducation.com
Good hunting! Barbara Foster Microscopy/Microscopy Education 125 Paridon Street, Suite 102 Springfield, MA 01118 PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^ Don't forget: the American Chem Society's Applied Optical Microscopy Course, March 5-7, Chicago, Ill. Visit our website for details and registration. NOT JUST FOR CHEMISTS!!!! ^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^ Best regards
At 07:27 AM 2/11/04 +1100, zeriena-at-yahoo.com wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
From MicroscopyL-request-at-ns.microscopy.com Sun Feb 15 17:55:47 2004
I am looking for the make and size of the Ion pump that is on the Philips CM12. It seems easier to ask rather than pull the covers off the scope as the EDS is in the way.
I am also looking for any used PSEM 75's for sale Please contact me at the below e-mail address.
Thanks in advance Hank Beebe
Manager Instrument Services hbeebe-at-rjlg.com RJLee Group, Inc. 350 Hochberg Rd. Monroeville, PA 15146 (724) 325-1776 voice (724) 733-1799 fax
From MicroscopyL-request-at-ns.microscopy.com Sun Feb 15 18:44:43 2004
} } 3DPTV 2004, Thessaloniki - Greece } http://www.umiacs.umd.edu/conferences/3dpvt04/ } } ============================================================================ } } Call for Papers } } The second International Symposium on 3DPTV (3D Data Processing, } Visualization, and } Transmission) will be held on September 6 to 9, 2004 in the city of } Thessaloniki, Greece. } } The goal of this meeting is to present and discuss new research ideas and } results related to the capture, representation, compact storage, } transmission, processing, editing, optimization and visualization or 3D } data. These topics span a number of research fields from applied } mathematics, computer science, and engineering: computer vision, computer } graphics, geometric modeling, signal and image processing, bionformatics, } and statistics. } } This symposium follows the highly successful 1st International Symposium on } 3D Data Processing, Visualization, and Transmission } 3DPVT'02 (http://www.dei.unipd.it/conferences/3DPVT) which took place in } 2003 in Padova, Italy. The proceedings of the symposium will be published in } the IEEE Proceedings Series, in cooperation with Eurographics and ACM } SIGGRAPH. } } A list of topics of interest includes but is not limited to: } } - 3D scanning technologies and devices } - 3D photography algorithms } - 3D view registration and surface modeling } - Surface reflectance recovery and modeling } - 3D texture processing } - Image-based rendering and modeling } - Multi-view image and geometry processing } - Stereo and motion reconstruction } - Augmented reality } - Compression, transmission and visualization of 3D data } - 3D Content-based retrieval and recognition } - Man/machine interaction with 3D data } - 3D printing and rapid prototyping } - Psychophysics of 3D sensing and haptics } - 3D imaging in biomedicine } - Structural analysis and pattern discovery in bioinformatics } - 3D imaging in virtual heritage and virtual archeology } - 3D imaging in e-commerce. } - 3D Television } - Teleimmersion and remote collaboration } } Paper submission } } Papers submitted for review must follow the IEEE CS Press Proceedings } two-column format. The papers must be submitted for review in final form. } The maximum paper length for review as well as for publication is 8 pages, } including the bibliography and the figures. Electronic manuscripts must be } submitted in Adobe Acrobat PDF format. In exceptional circumstances, } PostScript files will be accepted and converted to PDF: you must contact the } conference in advance if you intend to do so. } } The paper must have the full author contact information. All accepted papers } will be published in the Proceedings of the Symposium (on a CD-ROM). } The symposium language will be English. } } Important Dates } } Abstracts : April 2 } Full Papers : April 7 } Reviews due : May 15 } Author notification : May 25 } Deadline for price } reduced hotel } booking : June 10 } Camera-ready Papers : June 15 } and Registration of at } least one author per } paper } } Hotel reservations : May 25 to August 30 } Registration deadline : June 30 } for reduced price } } Tutorials : September 6 } Symposium : September 7-9 } } } } -------------------------------------------------------------------------- -- } --- } } General chairs } } - Aloimonos, Yiannis, University of Maryland, USA {yiannis-at-cfar.umd.edu} } - Taubin, Gabriel, Brown University, USA {taubin-at-brown.edu} } } Finance and registration chair } } - Duraiswami, Ramani, University of Maryland, {ramani-at-umiacs.umd.edu} } } Local arrangements } } - Petrou, Maria {M.Petrou-at-ee.surrey.ac.uk} } - Strintzis, Michael {strintzi-at-eng.auth.gr} } - Mpountanour, Kalliope {kalm-at-iti.gr} } - George Triantafyllidis {gatrian-at-iti.gr} } } Publication } } - Kimia, Ben, Brown University, USA {kimia-at-lems.brown.edu} } } } Publicity } } - Niovi Pavlidou, Aristotelian University of Thessaloniki } {niovi-at-vergina.eng.auth.gr} } } } } Steering Committee } } - Yiannis Aloimonos, University of Maryland, USA } - Guido Cortelazzo, University of Padova, Italy } - Concettina Guerra, University of Padova, Italy } - Avi Kak, Purdue University, USA } - Jan Koenderink, Utrecht University, Holland } - Pietro Perona, Caltech, USA } - Gabriel Taubin, Brown University, USA } - Luc Van Gool, University of Leuven-ETH Zentrum, Belgium-Switzerland } } Keynote speakers: } } Nadia Magnenat-Thalmann, Geneva } Vladimir Brajovic, CMU {brajovic-at-cs.cmu.edu} } Jan Koenderink, Utrecht {j.j.koenderink-at-phys.uu.nl} } Markus Gross, ETH Zurich {grossm-at-inf.ethz.ch} } Craig Gotsman, Harvard {gotsman-at-eecs.harvard.edu} } Demetri Terzopoulos, Courant {dt-at-cs.nyu.edu} } George Barbastathis, MIT {gbarb-at-mit.edu} } Andrew Fitzgibbon, Oxford } Patrick Cavanagh, Harvard (patrick-at-wjh.harvard.edu) } } } Special session organizers include: } } Gotsman, Craig (geometry processing) {gotsman-at-eecs.harvard.edu} } Pollefeys, Marc (multiple view geometry) {marc-at-cs.unc.edu} } } Tutorials include: } } Marc Pollefeys, 3D Photography } } If you are interested in giving a tutorial, please } contact the Chairs. } } Program committee: } } 1 Marc Alexa {alexa-at-informatik.tu-darmstadt.de} } 2 Nina Amenta {amenta-at-cs.ucdavis.edu} } 3 Anup Basu {anup-at-cs.ualberta.ca} } 4 Alexander Belyaev {belyaev-at-mpi-sb.mpg.de} } 5 Fausto Bernardini {fausto-at-us.ibm.com} } 6 Jean-Daniel Boissonat {Jean-Daniel.Boissonnat-at-inria.fr} } 7 Vladimir Brajovic {brajovic-at-cs.cmu.edu} } 8 Pere Brunet {pere-at-lsi.upc.es} } 9 Daniel Cohen-Or {dcor-at-tau.ac.il} } 10 David Cooper {cooper-at-lems.brown.edu} } 11 Guido Cortelazzo {corte-at-dei.unipd.it} } 12 Kostas Daniilidis {kostas-at-cis.upenn.edu} } 13 Larry Davis {lsd-at-umiacs.umd.edu } 14 Leila DeFloriani {deflo-at-disi.unige.it} } 15 Tamal Dey {tamaldey-at-cis.ohio-state.edu} } 16 Craig Gotsman {gotsman-at-eecs.harvard.edu} } 17 Markus Gross {grossm-at-inf.ethz.ch} } 18 Concettina Guerra {guerra-at-dei.unipd.it} } 19 Martial Hebert {hebert-at-ri.cmu.edu} } 20 David Jacobs {djacobs-at-cs.umd.edu} } 21 Avi Kak {kak-at-ecn.purdue.edu} } 22 Myung-Soo Kim {mskim-at-cse.snu.ac.kr } 23 Leif Kobbelt {kobbelt-at-cs.rwth-aachen.de} } 24 Jan Koenderink {j.j.koenderink-at-phys.uu.nl} } 25 Jana Kosecka {kosecka-at-cs.gmu.edu} } 26 Frederic Leymarie {leymarie-at-lems.brown.edu} } 27 Yi Ma {yima-at-uiuc.edu} } 28 Nadia Magnenat-Thalmann {thalmann-at-miralab.unige.ch} } 29 Roberto Manduchi {manduchi-at-soe.ucsc.edu} } 30 Dinesh Manocha {dm-at-cs.unc.edu} } 31 Ioana Martin {ioana-at-us.ibm.com} } 32 Ralph Martin {ralph-at-cs.cf.ac.uk} } 33 Takashi Matsuyama {tm-at-i.kyoto-u.ac.jp} } 34 Randal Nelson {nelson-at-cs.rochester.edu} } 35 Ko Nishino {kon-at-cs.columbia.edu} } 36 Valerio Pascucci {pascucci1-at-llnl.gov} } 37 Yannis Pitas {pitas-at-zeus.csd.auth.gr} } 38 Marc Pollefeys {marc-at-cs.unc.edu} } 39 Jean Ponce {ponce-at-cs.uiuc.edu} } 40 Martin Rumpf {rumpf-at-math.uni-duisburg.de} } 41 Holly Rushmeier {hertjwr-at-us.ibm.com} } 42 Szymon Rusinkiewicz {smr-at-cs.princeton.edu} } 43 Francis Schmitt {schmitt-at-ima.enst.fr} } 44 Peter Schroeder {ps-at-cs.caltech.edu} } 45 Hans-Peter Seidel {hpseidel-at-mpi-sb.mpg.de} } 46 Claudio Silva {csilva-at-cs.utah.edu} } 47 Yoshishisa Shinagawa {sinagawa-at-uiuc.edu} } 48 Harry Shum {hshum-at-microsoft.com} } 49 Stefano Soatto {soatto-at-cs.ucla.edu} } 50 Carlo Tomasi {tomasi-at-cs.duke.edu} } 51 Luc VanGool {vangool-at-vision.ee.ethz.ch} } 52 Luiz Velho {lvelho-at-impa.br} } 53 Denis Zorin {dzorin-at-mrl.nyu.edu} } 54 Naokazu Yokoya {yokoya-at-is.aist-nara.ac.jp} } 55 Peter Belhumeur {belhumeur-at-cs.columbia.edu} } 56 Brian Curless {curless-at-cs.washington.edu} } 57 Leonard McMillan {mcmillan-at-cs.unc.edu} } 58 Davi Geiger {geiger-at-cs.nyu.edu} } 59 Helder Jesus Araujo, Portugal } 60 Daniel Cremers, UCLA } 61 Nikos Paragios, Siemens/France } } _______________________________________________ } 3dpvt2004 mailing list } 3dpvt2004-at-umiacs.umd.edu } http://lists.umiacs.umd.edu/mailman/listinfo/3dpvt2004
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 16 02:42:20 2004
} } IASTED International Newsletter on Modelling and Simulation } February 3, 2004 } } UPCOMING DEADLINES } } 2 WEEKS REMAINING TO SUBMIT PAPERS } } 1. The IASTED International Conference on Applied Simulation and Modelling - ASM 2004 } June 28-30, 2004, Rhodes, Greece } } Important Deadlines: } **Submissions Due: Feb. 15, 2004** } Notification of Acceptance: Apr. 1, 2004 } Registration Deadline: May 1, 2004 } } To submit a paper, tutorial or special session or for more information visit our website at http://www.iasted.org/conferences/2004/greece/asm.htm } } **Special Session Announcement** } "Modelling and Simulation of Complex Biomechanical Systems" organized by Prof. Philippe Gorce, University of Toulon-Var, France and Dr. Philippe Pudlo, University of Valenciennes, France. } } } 2. The 4th IASTED International Conference on Modelling, Simulation, and Optimization - MSO 2004 } August 16-18, 2004, Kauai, Hawaii, USA } } Important Deadlines: } **Submissions Due: Mar. 5, 2004** } Notification of Acceptance: Apr. 15, 2004 } Registration Deadline: June 1, 2004 } To submit a paper, tutorial or special session or for more information visit our website at http://www.iasted.org/conferences/2004/hawaii/mso.htm } } UPCOMING MODELLING AND SIMULATION CONFERENCE } The submission deadline for the 15th IASTED International Conference on Modelling and Simulation - MS 2004 being held March 1-3, 2004 in Marina Del Rey, CA, USA has passed. Delegates without papers (attendees) are still welcome to register. } } For registration information visit our website at http://www.iasted.org/conferences/2004/marina/ms.htm } } MS 2004 Keynote Address } "Modeling and Simulation of Chemically Reactive Systems at High Pressure" by Dr. Francis H. Ree, Lawrence Livermore National Laboratory, Livermore, USA. For additional information visit http://www.iasted.org/conferences/2004/marina/ms-keynote.htm. } } MS 2004 Tutorial } "Simulation-Based Engineering of Complex Systems Using EXTEND+OpEMCSS" } Presented by Dr. John R. Clymer - California State University, Fullerton, USA } For additional information visit http://www.iasted.org/conferences/2004/marina/ms-tutorial1.htm } } MS 2004 Special Session } "Intelligent Reconfigurable Simulation" Organized by Dr. Tudor Niculiu -University "Politehnica" of Bucharest, Romania. For additional information visit http://www.iasted.org/conferences/2004/marina/ms-specialsessions.htm } } } JOURNAL SUBMISSION } Selected papers accepted to our conferences will be considered for review for inclusion in the IASTED International Journal of Modelling and Simulation (IJMS). Authors must submit an expanded version of their conference papers for peer review consideration to http://www.actapress.com/journals/submission.htm, following the standard procedure as described on the web site: www.actapress.com. All papers considered for peer review in the International Journal of Modelling and Simulation must be of the highest quality and demonstrate a novel contribution to the literature. } } } FUTURE CONFERENCES } Mark your calendars! The IASTED International Conference on Environmental Modelling and Simulation - EM&S 2004 will be held Nov. 22-24, 2004 in St. Thomas, Virgin Islands, USA. } } } MODELLING AND SIMULATION JOURNAL FROM ACTA PRESS } The International Journal of Modelling and Simulation - First published in 1981, this journal covers all aspects of modelling, simulation, languages, software, hardware, methodology, numerical and graphical methods, virtual reality, statistical techniques, tutorials, surveys, and applications. It also includes book reviews, conference notices, call for papers, and new publications. } Editor-in-Chief: Prof. A. Houshyar } Frequency: 4 issues per year } 2003 Rate: US$256.00 } Postage & Handling: US$25.00 } ISSN: 0228-6203 (205) } http://www.actapress.com/journals/journals.htm#Modelling } } } IASTED MEMBERSHIP } It pays to be a member! One of the benefits of your IASTED membership is a complimentary subscription to an ACTA Press journal of your choice. Become a member today! http://www.iasted.org/member/OnlineMembershipForm.pdf } } } CONFERENCE PROCEEDINGS AVAILABLE } Forward this information to your University library in order to stay up to date! Past conference proceedings in the area of modelling and simulation are available for purchase from ACTA Press - http://www.actapress.com/proceedings/proceedings.htm. } } For more information, to be removed from our mailing list, or to join one of the following mail groups: Power, Telecommunication, Bio-Medicine, Signal and Image Processing, Artificial Intelligence, Business, Software Engineering, Education, Databases and Knowledge Engineering, Internet and Applications, Parallel and Distributed Computing, please contact: } IASTED } #80, 4500 - 16th Avenue N.W. } Calgary, Alberta } Canada T3B 0M6 } Tel: 403-288-1195 } Fax: 403-247-6851 } E-mail: calgary-at-iasted.com } Web site: http://www.iasted.org }
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 16 05:08:52 2004
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I am looking for the make and size of the Ion pump that is on the Philips CM12. It seems easier to ask rather than pull the covers off the scope as the EDS is in the way.
I am also looking for any used PSEM 75's for sale Please contact me at the below e-mail address.
Thanks in advance Hank Beebe
Manager Instrument Services hbeebe-at-rjlg.com RJLee Group, Inc. 350 Hochberg Rd. Monroeville, PA 15146 (724) 325-1776 voice (724) 733-1799 fax
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 16 05:08:29 2004
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dear Rose,
Yes, the number after the magnification is the magnification is the Numerical Aperture or NA.
Three quick equations which you might find helpful.
1. NA = n sin a where n = refractive index of the immersing liquid between the top of the sample and the front element of the lens a = 1/2 of the collecting angle of the objective
From this equation, you can quickly see that, the larger the collecting angle, the greater the NA. Also, the refractive index of air is 1.00, so using immersing fluids like water (1.33), glycerin (1.47), or immersion oil (1.5212) has tremendous effect on improving the NA. Reminder: use only the fluid for which the lens is engineered. Trying to use immersion oil with a "dry" (air) objective, is a disaster. Lenses built for immersing liquids are specially sealed so that the liquid does not creep into the internal construction of the lenses.
2. Why is NA important? The reasons are derived from Diffraction Theory. Every object produces a diffraction pattern (see Young's slit experiments in any physics book for details). For simple objects like stage micrometers and diatoms like pleurasigma angulatum, you can see the diffraction pattern by removing the eyepiece and peering deep down the tube into the Back Focal Plane of the objective. (You also need to close down the condernser as far as it will go, to make the source of light a very small opening or, alternatively, remove it and put a pinhole over the light port in the base of your microscope). If the NA is big enough to capture 2 adjacent spots from the diffraction pattern, enough information is present to determine spacing in the original object. The basic equation for resolution summarizes:
Rxy = (1.22x wavelength)/ (NAobjective+NAcondenser). R = the size (in the XY plane, typically in micrometers) of the smallest resolvable particle 1.22 = a shape function (assuming round apertures in the microscope) Wavelength = you can use 500 nm or 0.500 micrometers as sort of the center of the visible spectrum NA = real working NAs (note: just because a lens is engraved with a value, does not mean that that is the working NA. Closing down an iris in the back focal plane of the objective or closing down the condenser aperture iris reduces the real working NA).
If the NA is big enough to capture THREE adjacent diffraction spots (the set of -1/0/+1 only counts as 2), you will begin to get better edge definition. So, the bigger the NA, the smaller the particle that you can resolve and the better the edge quality will be.
3. As for your other question: micrometers Are you referring to the working distance (distance from the front of the lense to the top of the sample) or Field of View? If working distance: you can either measure that directly or can look up the specs in the literature from the manufacturer. If Field of view (the diameter of the territory you see when looking through the eyepieces): F. O. V = (field number)/Mobjective The field number is the value written on the EYEPIECE, following the magnification. It refers to the distance across a small ridge or baffle typically mounted about 1/3 of the way up the inside wall from the BOTTOM of the eyepiece and typically ranges from about 18mm to 25 mm (old Reichert Polyvars and some others had ultra wide field of view and went to 32mm). Convert to micrometers by multiplying by 1000. Mobjective is the Magnification of the OBJECTIVE multiplied by any other auxiliary lenses between the objective and the bottom of the eyepiece. Ex: If you were working with eyepieces which had field number 22 (22 mm or 22,000 micrometers) and a 10x objective, the size of the field of view is simply 22,000/10 or 2,000micrometers.
Note: the eyepiece going to the camera is often a different magnification than the eyepieces you look through and the chip in the camera has its own equivalent of a "magnification", so what you see in the eyepieces will often be a larger FOV than you can capture with your camera.
You might find my book, "Optimizing Light Microscopy", helpful. A description and ordering information are on our website: www.MicroscopyEducation.com
Good hunting! Barbara Foster Microscopy/Microscopy Education 125 Paridon Street, Suite 102 Springfield, MA 01118 PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^ Don't forget: the American Chem Society's Applied Optical Microscopy Course, March 5-7, Chicago, Ill. Visit our website for details and registration. NOT JUST FOR CHEMISTS!!!! ^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^ Best regards
At 07:27 AM 2/11/04 +1100, zeriena-at-yahoo.com wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 16 05:13:33 2004
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } 3DPTV 2004, Thessaloniki - Greece } http://www.umiacs.umd.edu/conferences/3dpvt04/ } } ============================================================================ } } Call for Papers } } The second International Symposium on 3DPTV (3D Data Processing, } Visualization, and } Transmission) will be held on September 6 to 9, 2004 in the city of } Thessaloniki, Greece. } } The goal of this meeting is to present and discuss new research ideas and } results related to the capture, representation, compact storage, } transmission, processing, editing, optimization and visualization or 3D } data. These topics span a number of research fields from applied } mathematics, computer science, and engineering: computer vision, computer } graphics, geometric modeling, signal and image processing, bionformatics, } and statistics. } } This symposium follows the highly successful 1st International Symposium on } 3D Data Processing, Visualization, and Transmission } 3DPVT'02 (http://www.dei.unipd.it/conferences/3DPVT) which took place in } 2003 in Padova, Italy. The proceedings of the symposium will be published in } the IEEE Proceedings Series, in cooperation with Eurographics and ACM } SIGGRAPH. } } A list of topics of interest includes but is not limited to: } } - 3D scanning technologies and devices } - 3D photography algorithms } - 3D view registration and surface modeling } - Surface reflectance recovery and modeling } - 3D texture processing } - Image-based rendering and modeling } - Multi-view image and geometry processing } - Stereo and motion reconstruction } - Augmented reality } - Compression, transmission and visualization of 3D data } - 3D Content-based retrieval and recognition } - Man/machine interaction with 3D data } - 3D printing and rapid prototyping } - Psychophysics of 3D sensing and haptics } - 3D imaging in biomedicine } - Structural analysis and pattern discovery in bioinformatics } - 3D imaging in virtual heritage and virtual archeology } - 3D imaging in e-commerce. } - 3D Television } - Teleimmersion and remote collaboration } } Paper submission } } Papers submitted for review must follow the IEEE CS Press Proceedings } two-column format. The papers must be submitted for review in final form. } The maximum paper length for review as well as for publication is 8 pages, } including the bibliography and the figures. Electronic manuscripts must be } submitted in Adobe Acrobat PDF format. In exceptional circumstances, } PostScript files will be accepted and converted to PDF: you must contact the } conference in advance if you intend to do so. } } The paper must have the full author contact information. All accepted papers } will be published in the Proceedings of the Symposium (on a CD-ROM). } The symposium language will be English. } } Important Dates } } Abstracts : April 2 } Full Papers : April 7 } Reviews due : May 15 } Author notification : May 25 } Deadline for price } reduced hotel } booking : June 10 } Camera-ready Papers : June 15 } and Registration of at } least one author per } paper } } Hotel reservations : May 25 to August 30 } Registration deadline : June 30 } for reduced price } } Tutorials : September 6 } Symposium : September 7-9 } } } } -------------------------------------------------------------------------- -- } --- } } General chairs } } - Aloimonos, Yiannis, University of Maryland, USA {yiannis-at-cfar.umd.edu} } - Taubin, Gabriel, Brown University, USA {taubin-at-brown.edu} } } Finance and registration chair } } - Duraiswami, Ramani, University of Maryland, {ramani-at-umiacs.umd.edu} } } Local arrangements } } - Petrou, Maria {M.Petrou-at-ee.surrey.ac.uk} } - Strintzis, Michael {strintzi-at-eng.auth.gr} } - Mpountanour, Kalliope {kalm-at-iti.gr} } - George Triantafyllidis {gatrian-at-iti.gr} } } Publication } } - Kimia, Ben, Brown University, USA {kimia-at-lems.brown.edu} } } } Publicity } } - Niovi Pavlidou, Aristotelian University of Thessaloniki } {niovi-at-vergina.eng.auth.gr} } } } } Steering Committee } } - Yiannis Aloimonos, University of Maryland, USA } - Guido Cortelazzo, University of Padova, Italy } - Concettina Guerra, University of Padova, Italy } - Avi Kak, Purdue University, USA } - Jan Koenderink, Utrecht University, Holland } - Pietro Perona, Caltech, USA } - Gabriel Taubin, Brown University, USA } - Luc Van Gool, University of Leuven-ETH Zentrum, Belgium-Switzerland } } Keynote speakers: } } Nadia Magnenat-Thalmann, Geneva } Vladimir Brajovic, CMU {brajovic-at-cs.cmu.edu} } Jan Koenderink, Utrecht {j.j.koenderink-at-phys.uu.nl} } Markus Gross, ETH Zurich {grossm-at-inf.ethz.ch} } Craig Gotsman, Harvard {gotsman-at-eecs.harvard.edu} } Demetri Terzopoulos, Courant {dt-at-cs.nyu.edu} } George Barbastathis, MIT {gbarb-at-mit.edu} } Andrew Fitzgibbon, Oxford } Patrick Cavanagh, Harvard (patrick-at-wjh.harvard.edu) } } } Special session organizers include: } } Gotsman, Craig (geometry processing) {gotsman-at-eecs.harvard.edu} } Pollefeys, Marc (multiple view geometry) {marc-at-cs.unc.edu} } } Tutorials include: } } Marc Pollefeys, 3D Photography } } If you are interested in giving a tutorial, please } contact the Chairs. } } Program committee: } } 1 Marc Alexa {alexa-at-informatik.tu-darmstadt.de} } 2 Nina Amenta {amenta-at-cs.ucdavis.edu} } 3 Anup Basu {anup-at-cs.ualberta.ca} } 4 Alexander Belyaev {belyaev-at-mpi-sb.mpg.de} } 5 Fausto Bernardini {fausto-at-us.ibm.com} } 6 Jean-Daniel Boissonat {Jean-Daniel.Boissonnat-at-inria.fr} } 7 Vladimir Brajovic {brajovic-at-cs.cmu.edu} } 8 Pere Brunet {pere-at-lsi.upc.es} } 9 Daniel Cohen-Or {dcor-at-tau.ac.il} } 10 David Cooper {cooper-at-lems.brown.edu} } 11 Guido Cortelazzo {corte-at-dei.unipd.it} } 12 Kostas Daniilidis {kostas-at-cis.upenn.edu} } 13 Larry Davis {lsd-at-umiacs.umd.edu } 14 Leila DeFloriani {deflo-at-disi.unige.it} } 15 Tamal Dey {tamaldey-at-cis.ohio-state.edu} } 16 Craig Gotsman {gotsman-at-eecs.harvard.edu} } 17 Markus Gross {grossm-at-inf.ethz.ch} } 18 Concettina Guerra {guerra-at-dei.unipd.it} } 19 Martial Hebert {hebert-at-ri.cmu.edu} } 20 David Jacobs {djacobs-at-cs.umd.edu} } 21 Avi Kak {kak-at-ecn.purdue.edu} } 22 Myung-Soo Kim {mskim-at-cse.snu.ac.kr } 23 Leif Kobbelt {kobbelt-at-cs.rwth-aachen.de} } 24 Jan Koenderink {j.j.koenderink-at-phys.uu.nl} } 25 Jana Kosecka {kosecka-at-cs.gmu.edu} } 26 Frederic Leymarie {leymarie-at-lems.brown.edu} } 27 Yi Ma {yima-at-uiuc.edu} } 28 Nadia Magnenat-Thalmann {thalmann-at-miralab.unige.ch} } 29 Roberto Manduchi {manduchi-at-soe.ucsc.edu} } 30 Dinesh Manocha {dm-at-cs.unc.edu} } 31 Ioana Martin {ioana-at-us.ibm.com} } 32 Ralph Martin {ralph-at-cs.cf.ac.uk} } 33 Takashi Matsuyama {tm-at-i.kyoto-u.ac.jp} } 34 Randal Nelson {nelson-at-cs.rochester.edu} } 35 Ko Nishino {kon-at-cs.columbia.edu} } 36 Valerio Pascucci {pascucci1-at-llnl.gov} } 37 Yannis Pitas {pitas-at-zeus.csd.auth.gr} } 38 Marc Pollefeys {marc-at-cs.unc.edu} } 39 Jean Ponce {ponce-at-cs.uiuc.edu} } 40 Martin Rumpf {rumpf-at-math.uni-duisburg.de} } 41 Holly Rushmeier {hertjwr-at-us.ibm.com} } 42 Szymon Rusinkiewicz {smr-at-cs.princeton.edu} } 43 Francis Schmitt {schmitt-at-ima.enst.fr} } 44 Peter Schroeder {ps-at-cs.caltech.edu} } 45 Hans-Peter Seidel {hpseidel-at-mpi-sb.mpg.de} } 46 Claudio Silva {csilva-at-cs.utah.edu} } 47 Yoshishisa Shinagawa {sinagawa-at-uiuc.edu} } 48 Harry Shum {hshum-at-microsoft.com} } 49 Stefano Soatto {soatto-at-cs.ucla.edu} } 50 Carlo Tomasi {tomasi-at-cs.duke.edu} } 51 Luc VanGool {vangool-at-vision.ee.ethz.ch} } 52 Luiz Velho {lvelho-at-impa.br} } 53 Denis Zorin {dzorin-at-mrl.nyu.edu} } 54 Naokazu Yokoya {yokoya-at-is.aist-nara.ac.jp} } 55 Peter Belhumeur {belhumeur-at-cs.columbia.edu} } 56 Brian Curless {curless-at-cs.washington.edu} } 57 Leonard McMillan {mcmillan-at-cs.unc.edu} } 58 Davi Geiger {geiger-at-cs.nyu.edu} } 59 Helder Jesus Araujo, Portugal } 60 Daniel Cremers, UCLA } 61 Nikos Paragios, Siemens/France } } _______________________________________________ } 3dpvt2004 mailing list } 3dpvt2004-at-umiacs.umd.edu } http://lists.umiacs.umd.edu/mailman/listinfo/3dpvt2004
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 16 05:13:32 2004
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} } IASTED International Newsletter on Modelling and Simulation } February 3, 2004 } } UPCOMING DEADLINES } } 2 WEEKS REMAINING TO SUBMIT PAPERS } } 1. The IASTED International Conference on Applied Simulation and Modelling - ASM 2004 } June 28-30, 2004, Rhodes, Greece } } Important Deadlines: } **Submissions Due: Feb. 15, 2004** } Notification of Acceptance: Apr. 1, 2004 } Registration Deadline: May 1, 2004 } } To submit a paper, tutorial or special session or for more information visit our website at http://www.iasted.org/conferences/2004/greece/asm.htm } } **Special Session Announcement** } "Modelling and Simulation of Complex Biomechanical Systems" organized by Prof. Philippe Gorce, University of Toulon-Var, France and Dr. Philippe Pudlo, University of Valenciennes, France. } } } 2. The 4th IASTED International Conference on Modelling, Simulation, and Optimization - MSO 2004 } August 16-18, 2004, Kauai, Hawaii, USA } } Important Deadlines: } **Submissions Due: Mar. 5, 2004** } Notification of Acceptance: Apr. 15, 2004 } Registration Deadline: June 1, 2004 } To submit a paper, tutorial or special session or for more information visit our website at http://www.iasted.org/conferences/2004/hawaii/mso.htm } } UPCOMING MODELLING AND SIMULATION CONFERENCE } The submission deadline for the 15th IASTED International Conference on Modelling and Simulation - MS 2004 being held March 1-3, 2004 in Marina Del Rey, CA, USA has passed. Delegates without papers (attendees) are still welcome to register. } } For registration information visit our website at http://www.iasted.org/conferences/2004/marina/ms.htm } } MS 2004 Keynote Address } "Modeling and Simulation of Chemically Reactive Systems at High Pressure" by Dr. Francis H. Ree, Lawrence Livermore National Laboratory, Livermore, USA. For additional information visit http://www.iasted.org/conferences/2004/marina/ms-keynote.htm. } } MS 2004 Tutorial } "Simulation-Based Engineering of Complex Systems Using EXTEND+OpEMCSS" } Presented by Dr. John R. Clymer - California State University, Fullerton, USA } For additional information visit http://www.iasted.org/conferences/2004/marina/ms-tutorial1.htm } } MS 2004 Special Session } "Intelligent Reconfigurable Simulation" Organized by Dr. Tudor Niculiu -University "Politehnica" of Bucharest, Romania. For additional information visit http://www.iasted.org/conferences/2004/marina/ms-specialsessions.htm } } } JOURNAL SUBMISSION } Selected papers accepted to our conferences will be considered for review for inclusion in the IASTED International Journal of Modelling and Simulation (IJMS). Authors must submit an expanded version of their conference papers for peer review consideration to http://www.actapress.com/journals/submission.htm, following the standard procedure as described on the web site: www.actapress.com. All papers considered for peer review in the International Journal of Modelling and Simulation must be of the highest quality and demonstrate a novel contribution to the literature. } } } FUTURE CONFERENCES } Mark your calendars! The IASTED International Conference on Environmental Modelling and Simulation - EM&S 2004 will be held Nov. 22-24, 2004 in St. Thomas, Virgin Islands, USA. } } } MODELLING AND SIMULATION JOURNAL FROM ACTA PRESS } The International Journal of Modelling and Simulation - First published in 1981, this journal covers all aspects of modelling, simulation, languages, software, hardware, methodology, numerical and graphical methods, virtual reality, statistical techniques, tutorials, surveys, and applications. It also includes book reviews, conference notices, call for papers, and new publications. } Editor-in-Chief: Prof. A. Houshyar } Frequency: 4 issues per year } 2003 Rate: US$256.00 } Postage & Handling: US$25.00 } ISSN: 0228-6203 (205) } http://www.actapress.com/journals/journals.htm#Modelling } } } IASTED MEMBERSHIP } It pays to be a member! One of the benefits of your IASTED membership is a complimentary subscription to an ACTA Press journal of your choice. Become a member today! http://www.iasted.org/member/OnlineMembershipForm.pdf } } } CONFERENCE PROCEEDINGS AVAILABLE } Forward this information to your University library in order to stay up to date! Past conference proceedings in the area of modelling and simulation are available for purchase from ACTA Press - http://www.actapress.com/proceedings/proceedings.htm. } } For more information, to be removed from our mailing list, or to join one of the following mail groups: Power, Telecommunication, Bio-Medicine, Signal and Image Processing, Artificial Intelligence, Business, Software Engineering, Education, Databases and Knowledge Engineering, Internet and Applications, Parallel and Distributed Computing, please contact: } IASTED } #80, 4500 - 16th Avenue N.W. } Calgary, Alberta } Canada T3B 0M6 } Tel: 403-288-1195 } Fax: 403-247-6851 } E-mail: calgary-at-iasted.com } Web site: http://www.iasted.org }
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 16 14:06:48 2004
We are in need of a working Oxford XP3 pulse processor. Please email with details and availability. Thanks, Ken -- Kenneth JT Livi, Ph.D. Department of Earth and Planetary Sciences 3400 N. Charles St. Johns Hopkins University Baltimore, MD 21218 (410) 516-8342 (410) 516-7933 fax
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 17 04:01:28 2004
Dear all, I am trying to open files obtained with a Noran confocal microscope (.mv file)with Image J, a software freely available on the net and equivalent to NIH image but running on a PC. Would anybody have any idea as how to do that ? Thanks for any help, Hervé.
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 17 08:30:54 2004
I am in possession of a movie by Hitachi that is over 25 years old and features the HU-11E TEM. I am about to toss it. If there is anyone out there who would like to have it, let me know and I will be happy to send it to you.
Greg Erdos
Gregory W. Erdos Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 gwe-at-ufl.edu 352-392-1295 fax- 352-846-0251
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 17 10:26:56 2004
It's been so long since we dumped the Noran that I don't remember the details, but I can tell you this:
I think the files were 512 X 484.
The header was of variable length consisting of keywords, that you can see by ASCII, followed by the parameter value.
I typically did the math of 484 X 512 X Z and then subtracted off the remainder as the header. Iterating on possible Z's finds the size.
} Dear all, } I am trying to open files obtained with a Noran confocal microscope (.mv } file)with Image J, a software freely available on the net and equivalent } to NIH image but running on a PC. Would anybody have any idea as how to } do that ? } Thanks for any help, } Hervé.
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 17 10:46:37 2004
The movie that I offered earlier today has been claimed by a lucky individual. I never thought that it would generate so much interest.
Gregory W. Erdos Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 gwe-at-ufl.edu 352-392-1295 fax- 352-846-0251
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 17 11:03:55 2004
My name is Thomas Sadowski and I am currently a Computer Science and Physics majorat Southern Connecticut State University. I am persuing an independent study in the field of CT Scanning more specifically micro CT and invivo CT scanning. I was wondering if anybody knew of any research sights, individuals, or sources that would be able to provide me with any amount of information concerning the process of these types of scans, the physics behind them, or the data collection and interpretation process.
Thank you in advance for any help that you can provide.
Thomas Sadowski Southern Connecticut State University
_________________________________________________________________ Stay informed on Election 2004 and the race to Super Tuesday. http://special.msn.com/msn/election2004.armx
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 17 12:17:14 2004
we would love to have it for the reference library here at the museum.
Thanks!
Ed Sharpe Archivist for SMECC
See the Southwest Museum of Engineering, Communications and Computation online at: http://www.smecc.org ----- Original Message ----- } From: "Greg Erdos" {gwe-at-biotech.ufl.edu} To: {Microscopy-at-sparc5.microscopy.com} Sent: Tuesday, February 17, 2004 7:41 AM
Have you tried the library? There are books, journals and websites on this topic.
Geoff
Thomas Sadowski wrote:
} ------------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } } Hello, } } My name is Thomas Sadowski and I am currently a Computer Science and } Physics majorat Southern Connecticut State University. I am persuing } an independent study in the field of CT Scanning more specifically } micro CT and invivo CT scanning. I was wondering if anybody knew of } any research sights, individuals, or sources that would be able to } provide me with any amount of information concerning the process of } these types of scans, the physics behind them, or the data collection } and interpretation process. } } Thank you in advance for any help that you can provide. } } } Thomas Sadowski } Southern Connecticut State University } } _________________________________________________________________ } Stay informed on Election 2004 and the race to Super Tuesday. } http://special.msn.com/msn/election2004.armx } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 17 16:50:52 2004
Application form: Application Deadline: March 1, 2004 Optical Microscopy Course Notification of Admission: March 15,2004 At The University of Texas Health Science Center at San Antonio June 2-9, 2004
_________________________________________________________________ Phone: ______________________________ Fax:______________________________ Email:________________________________ Degree: _______ Academic Yes / No Commercial Yes / No
Please consider my application for one of the 4 available scholarships. Yes / No
Note that Registration for the FRET/FLIM/Spectral Imaging Symposium is included in the tuition.
Upon admission to the Optical Microscopy Course you will be required to submit payment $2100 (US) on or before May 3, 2004 to reserve your spot in the workshop. This fee includes workshop material, symposium registration, room and board for June 2-9, 2004.
Briefly describe your interests in Optical Microscopy an how attending this course will further your research efforts.
Return to: Microscopy Course, Mail Code 7762 7703 Floyd Curl Dr. San Antonio, TX 78229-3900 Fax to: (210) 567-3803 Email to: Frohlich-at-uthscsa.edu
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 17 17:06:40 2004
You can export movies from ImageJ in AVI format if you use the AVI writer plugin, so I would suggest that you use that in future and export to AVI rather than save as .mv files. You may be able to open the .mv file in VirtualDub (http://www.virtualdub.com/) but I'm not sure. If so, you can subsequently write to AVI.
Good luck
Cheers,
Jacqui.
haptel-at-univ-montp2.fr wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Tel: 64 9 373 7599 Ext 87438 Fax: 64 9 373 7484
http://www.health.auckland.ac.nz/biru/
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 18 07:38:29 2004
I clean a window of a PRIZM detector systematically with Vertel XF solvent (according to manufacturer's instructions). I let detector to warm up, dismount it, and slowly put solvent drop after drop on the top of the metal ring surrounding the window, so that solvent will run over window. The window is very fragile, it could be damaged by single drop, if it goes directly on the window.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 3127 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} We use PGT eds detector and the light elements detection has decreased } significantly. Has anyone cleaned the detectors window? Is it } possible? } } Pavel Lozovyy } ATC SEM Lab } (216)692-6637 } www.atclabs.com } } } } } }
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 18 11:37:00 2004
I would appreciate any information concerning an introductory TEM training/course (USA) in the first or early in the second quarter of 2004. We need to get the trainee up to speed quickly and the Lehigh course in mid-June occurs too late in the year for our needs.
TIA
Fred A. Stewart-Davis Engineering Specialist Materials Characterization Lab Glass Technology Center Harmarville, PA 15238 fstewartdavis-at-ppg.com ************************************************************************* ************************************************************************* *************************************************************************
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 18 12:26:02 2004
Take a look at this website. It is an introductory online course on TEM Basics http://www.matter.org.uk/tem/
And the following is an "on-site" training/lecture course (You would probably have to call or e-mail to find out dates available): http://www.emcourses.com/lecture1.htm
Kelly A. Ramos Metallurgical Engineer / Supervisor Argo-Tech Materials Laboratories 23555 Euclid Avenue Cleveland, OH 44117 216-692-5904 or 216-692-5446 (fax) 216-692-5816 http://www.atclabs.com
"Stewart-Davis , Fred A." To: "'Microscopy-at-MSA.Microscopy.Com'" {fstewartdavis {Microscopy-at-MSA.Microscopy.Com} -at-ppg.com} cc: Subject: [Microscopy] TEM Courses/Training 02/18/04 12:47 PM
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I would appreciate any information concerning an introductory TEM training/course (USA) in the first or early in the second quarter of 2004. We need to get the trainee up to speed quickly and the Lehigh course in mid-June occurs too late in the year for our needs.
TIA
Fred A. Stewart-Davis Engineering Specialist Materials Characterization Lab Glass Technology Center Harmarville, PA 15238 fstewartdavis-at-ppg.com ************************************************************************* ************************************************************************* *************************************************************************
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 18 13:55:02 2004
I have been cleaning Be windows for years now using a similar technique (but with Freon) but have often wondered if this would be OK if I had an UTW.
Some years ago, before UTWs, Oxford used to recommend dipping the end of the detector into a beaker of ethanol.
cheers
rtch
} --------- } } I clean a window of a PRIZM detector systematically with } Vertel XF solvent (according to manufacturer's instructions). } I let detector to warm up, dismount it, and slowly put solvent } drop after drop on the top of the metal ring surrounding } the window, so that solvent will run over window. The window } is very fragile, it could be damaged by single drop, } if it goes directly on the window. } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 3127 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } We use PGT eds detector and the light elements detection has } } decreased significantly. Has anyone cleaned the detectors window? Is } } it possible? } } } } Pavel Lozovyy } } ATC SEM Lab } } (216)692-6637 } } www.atclabs.com } }
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 18 13:59:31 2004
} } Is this on an UTW or Be? } } I have been cleaning Be windows for years now using a similar } technique (but with } Freon) but have often wondered if this would be OK if I had an UTW. } } Some years ago, before UTWs, Oxford used to recommend dipping } the end of the } detector into a beaker of ethanol. } } cheers } } rtch } } } } } --------- } } } } I clean a window of a PRIZM detector systematically with } } Vertel XF solvent (according to manufacturer's instructions). } } I let detector to warm up, dismount it, and slowly put solvent } } drop after drop on the top of the metal ring surrounding } } the window, so that solvent will run over window. The window } } is very fragile, it could be damaged by single drop, } } if it goes directly on the window. } } } } Vladimir M. Dusevich, Ph.D. } } Electron Microscope Lab Manager } } 3127 School of Dentistry } } 650 E. 25th Street } } Kansas City, MO 64108-2784 } } } } Phone: (816) 235-2072 } } Fax: (816) 235-5524 } } Web: http://www.umkc.edu/dentistry/microscopy } } } } } We use PGT eds detector and the light elements detection has } } } decreased significantly. Has anyone cleaned the detectors } window? Is } } } it possible? } } } } } } Pavel Lozovyy } } } ATC SEM Lab } } } (216)692-6637 } } } www.atclabs.com } } } } } -- } Ritchie Sims Ph D Phone : 64 9 } 3737599 ext 87713 } Microanalyst Fax : } 64 9 3737435 } Department of Geology email : } r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } }
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 18 14:30:00 2004
This year we will be hosting the 9th Annual RMC Materials Microtomy Short Course in Tucson, Arizona from April 27 - 30.
Join us and our internationally renowned course faculty in sunny Tucson to participate in this unique event.
This short course is designed specifically for researchers in the field of materials science who wish to gain exposure to advances in specimen preparation for electron microscopy.
E-mail Kim Megaw at {kim-at-boeckeler.com} to receive full details and a course brochure.
Best Regards,
Robert (Bob) Chiovetti Senior Product Specialist RMC Products Boeckeler Instruments, Inc. 4650 South Butterfield Drive Tucson, AZ 85714 USA Tel. 520.745.0001 Fax 520.745.0004 {www.rmcproducts.com}
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 18 17:56:51 2004
DESCRIPTION OF POSITION: This job mainly involves using FIB to make TEM samples. However, the applicant should also be able to perform routine technical tasks in support of materials analysis activities. Should be skilled in optical microscopy, electron microscopy, micro cross-sectioning, FIB, wet/dry deprocessing. Specific site or defect locations FA. Supports material analysis through direct customer interaction.
SPECIFIC JOB FUNCTIONS: TEM sample prep by FIB. Close interaction with TEM engineers and requesting customers. Good computer skills needed.
PREFERRED EDUCATION AND EXPERIENCE: AS degree in a science field. Some semiconductor is preferred. Experience in the theory and operation of scanning electron microscopes and focussed ion beams is a real plus. Good manual skills for fine detailed work is required. Resourcefulness. Independent worker that can complete tasks with limited guidence. Should know PC's and internet.
For faster consideration, send your resume to jazylette.windell-at-amd.com and please cc:jobs-at-spansion.com. (Please do not send resumes to alline-at-earthlink.net; you may send them instead to alline.myers-at-spansion.com.)
Requisition Number FC52734
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 01:42:40 2004
The are some courses in Sheffield, UK in April. Maybe they can be of interest. See: http://www.microscopie.nl/19-23April.pdf
Erik Johnson Department of Materials Research Risø DK-4000 Roskilde Denmark
-----Original Message----- } From: Stewart-Davis, Fred A. [mailto:fstewartdavis-at-ppg.com] Sent: 18 February 2004 18:47 To: 'Microscopy-at-MSA.Microscopy.Com'
Hi, I have processed a large batch of mouse femurs (after decalcification) into epoxy resin for LM examination only. I have done this before and infiltration was good throughout the thickness of the sample (which can be 3mm max dimension). This last time a proportion of them are poorly infiltrated (air spaces) and the resin internally is a bit soft and sticky. Does anyone know if there is anyway I can remove the polymerised resin and attempt to get more in now that I have removed half the block by sectioning, (the actual tissue preservation looks fine)?
Thanks in anticipation
Gill Brown
Histopathology Group Asthma and Allergy Disease Biology ri- CEDD. GlaxoSmithKline Medicines Research Centre,
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 07:07:50 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sswaffe-at-abv.bg) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Tuesday, February 17, 2004 at 12:38:16 ---------------------------------------------------------------------------
Email: sswaffe-at-abv.bg Name: Veselin
Organization: 54th. st.Ivan Rilski
Education: 6-8th Grade Middle School
Location: Sofia, Bulgaria
Question: What are actually infininity optical systems and how do they differentiate from the normal optical systems?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (EPablo-at-Polese.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 18, 2004 at 14:10:15 ---------------------------------------------------------------------------
Question: I am looking for ASTM E112 (Order #PCN 12-501122-28) or equivalent to determine average grain size. Does anyone have any information where I can buy or get a copy? This item is no longer available from ASTM. Anyone that can provide information for this item is greatly appreciated. Thank you in advance
Elmer Pablo Materials Lab. Tech Polese Co. San Diego, Ca 858-348-1202 (Phone) 413-513-2527 (EFax)
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jderyk-at-dpi.radiology.uiowa.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 18, 2004 at 15:32:49 ---------------------------------------------------------------------------
Question: Hi, I was hoping someone could help me find, what I thought would be fundamental relationships for microscopy but are proving to be difficult to determine. I have a LeicaMz16a steromicroscope with; 7.11x to 155x mag FOV range: 29.5mm to 1.82mm
This is coupled to a CCD camera with; 1300x1030 pixel array pixel spacing: 6.7microns FOW: 8.7 x 6.9 mm
I'm wondering how I can accurately calculate the effective FOV as seen by the rectangular CCD. Also the relationship between magnification in the microscope and resulting pixel spacing of my digital images??
I would appreciate any advice in these calculations or references to texts which may contain information regarding interaction between CCD cameras and microscopes. Thanks Jess
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Julie.Glasscock-at-csiro.au) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 18, 2004 at 23:09:51 ---------------------------------------------------------------------------
Title-Subject: [Microscopy] [Filtered] Faraday cup
Question: I am using a JEOL 6300F SEM for e-beam lithography using Nabity's Nano-Pattern Generation System (NPGS). I need to use a Faraday cup to check the beam current. Apparently there is a Faraday cup attachment (on a movable arm) in our SEM that was purchased from Oxford Instruments. Unfortunately no-one here or at Oxford has any memory of where the attachment is placed or how to use it. We have found a BNC connector coming out of the specimen exchange chamber that may be the connection for an ammeter. Does anyone else have such an attachment and know where it may be located within the SEM and how it works? Any information will be gratefully received!
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amy.snodgrass-at-eyetk.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, February 17, 2004 at 14:16:55 ---------------------------------------------------------------------------
Question: I would like to buy a used sputter coater for coating SEM samples with metal and carbon. Anybody selling one? Boston/New England area preferred.
You might be able to remove it by soaking the blocks in sodium ethoxide and then reprocessing from absolute alcohol into new epoxy. There seems to be a possibility that this will affect antigens, though.
Lesley Weston.
on 19/02/2004 12:58 AM, gillian.2.brown-at-gsk.com at gillian.2.brown-at-gsk.com wrote:
} } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- --} - } } Hi, } I have processed a large batch of mouse femurs (after decalcification) } into epoxy resin for LM examination only. I have done this before and } infiltration was good throughout the thickness of the sample (which can be } 3mm max dimension). This last time a proportion of them are poorly } infiltrated (air spaces) and the resin internally is a bit soft and } sticky. Does anyone know if there is anyway I can remove the polymerised } resin and attempt to get more in now that I have removed half the block by } sectioning, (the actual tissue preservation looks fine)? } } Thanks in anticipation } } Gill Brown } } } Histopathology Group } Asthma and Allergy Disease Biology } ri- CEDD. } GlaxoSmithKline Medicines Research Centre, } } }
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 09:15:24 2004
This standard is active and available from ASTM. It can be ordered on line with a credit card direct from ASTM. Go to www.astm.org, click on Standards on the left menu, then click on Individual Standards to get the Standard Search form.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 10:51:05 2004
Until recently we were running a PGT thin window detector on an ISI-40 SEM. We ran it for about 13 years. Because it was on a diffusion pumped system it was necessary to clean the window periodically. Given our usage, relatively low hours, I found it necessary to clean the window about every four to six months. I did it when the copper L lines decreased in height to approach the Kalpha line height. I consulted PGT and found that it is important to 1). Clean it after warming up the system, 2) use a solvent that is compatible with the adhesive used on the window, 3) don't touch the window. It would take about 8 to 9 days for our dewar to warm after a fill. I did not remove the detector from the SEM. I used an eye dropper to drip a few drops of solvent on to the end of the detector. I was also able to "squirt" a few drops onto the window directly. I had occasion to employ this procedure probably 20 times or more. We retired the system, working very well because we acquired a new SEM that, incidentally has a silicon drift detector, LN2-free, mounted on it. I would recommend that before you do any cleaning contact PGT about your detector to get their advice as to which solvent is best for the window on your detector.
Vern Griffiths, Montana Tech, Emeritus Prof.
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 11:18:07 2004
I am currently loocking at liquid polymers in which DNA has been incorporated. In order to look at the resulting structures, I have tried several negative stains such as PTA, uranyl acetate, ammonium molybdate with different results and none of which really satisfy me.
For exemple, I have difficulties getting the sample to actually stay on the formvar grid, most of it seems to slip away... The method I use is the following; sample sitting on the formvar grid for 1-2 minutes drain the excess with a filter paper appl the stain for 1 minute drain the excess with a filter paper
I do not have access to a high vacuum evaporator (shadowing technique) but someone told me about using colloidal carbon mix with the liquid polymer sample, similar to negative staining. Has anyone heard about that technique and, if it is the case, what are the propotion of carbon, the best way to get good results..etc..
Other ideas are also welcome!!
thank you,
Diane
Diane Montpetit Microscopie électronique/Electron microscopy Centre de Recherche et de Développement sur les Aliments/Food and Development Research Center Agriculture et Agroalimentaire Canada/Agriculture and Agri-Food Canada téléphone/telephone 450-778-3024 (196) télécopieur/facsimile 450-773-8461 3600 Boul. Casavant Ouest/3600 Casavant West boul. St-Hyacinthe (Québec) J2S 8E3 montpetitd-at-agr.gc.ca
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 11:48:56 2004
I am looking for ASTM E112 (Order #PCN 12-501122-28) or equivalent to determine average grain size. Does anyone have any information where I can buy or get a copy? This item is no longer available from ASTM. Anyone that can provide information for this item is greatly appreciated. Thank you in advance.
Email: EPablo-at-Polese.com Name: Elmer Pablo Organization: Polese Company Inc.
Elmer Pablo Materials Lab. Tech Polese Co. San Diego, Ca 858-348-1202 (Phone)
Elmer, I have a copy of ASTM E 112-96. Check your e-mail and send me an address.
Stu Smalinskas Metallurgist SKF USA Plymouth, Michigan
__________________________________ Do you Yahoo!? Yahoo! Mail SpamGuard - Read only the mail you want. http://antispam.yahoo.com/tools
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 12:46:24 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tgreco-at-seas.marine.usf.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, February 19, 2004 at 12:14:55 ---------------------------------------------------------------------------
Email: tgreco-at-seas.marine.usf.edu Name: Tony Greco
Organization: University of South Florida
Title-Subject: [Microscopy] [Filtered] MListserver:Variable pressure SEM
Question: I purchased a variable pressure SEM with diffusion pump about 3 years ago and was told that by setting the sample chamber pressure to between 30-40Pa over the weekend, I could effectively scrub the inside of the chamber including any oil that had accumulated on the x-ray window. Sadly this has not been the case as chamber and especially the x-ray window regularly becomes contaminated with rotary pump oil. All efforts to combat this problem have failed including a foreline trap in the RP line. Any suggestions?
} } Title-Subject: [Microscopy] [Filtered] Faraday cup } } Question: I am using a JEOL 6300F SEM for e-beam lithography using } Nabity's Nano-Pattern Generation System (NPGS). I need to use a } Faraday cup to check the beam current. Apparently there is a Faraday } cup attachment (on a movable arm) in our SEM that was purchased from } Oxford Instruments. Unfortunately no-one here or at Oxford has any } memory of where the attachment is placed or how to use it. We have } found a BNC connector coming out of the specimen exchange chamber that } may be the connection for an ammeter. Does anyone else have such an } attachment and know where it may be located within the SEM and how it } works? Any information will be gratefully received! } }
I suggest you contact JEOL in Sydney.
JEOL probably have a PCD accessory for the 6300, which will fit to the scope better than will any third-party one, and Vitaly Lozbin will no doubt be able to identify your BNC connector over the phone.
They were even able to make me one for my 840, even though it was not in their current catalogue. At a very reasonable price.
cheers
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 13:46:04 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (JAHMIT-at-aol.com) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Thursday, February 19, 2004 at 13:41:24 ---------------------------------------------------------------------------
Email: JAHMIT-at-aol.com Name: Natalie Heltman
Organization: E.H. Greene
Education: K-8 Grade Grammar School
Location: Cincinnati, OH 45242
Question: Hello, I'm doing a research paper on John William Coleman and I cannot find any information on him. I was in need of any info. you can provide. I know that he was a member of Electron Microscopy Society? What was his role in the development of the electron microscope with RCA Labs?
Any info. would help I'm sending this message form my Dad's work place. Thanks, Natalie
..has some information about John William Coleman.
Here is another little bio I found:
JOHN WILLIAM COLEMAN (1929- ) was born in New York City, earning a Ph.D. in Biophysics from the University of Pennsylvania in 1963. From 1951 to 1953, John Coleman served as a Physicist for the National Bureau of Standards. He was an Instructor in Physics at Howard University from 1957-58, later becoming an Engineer for RCA in 1958. His research involved the physics of electrons, and he assisted in the development of the American electron microscope developed at RCA.
The above Princeton website also has a bibliography:
American Men of Science. 11th edition, Supplement 2 (New York: McGraw-Hill), p. 158.
Blacks in Science and Education. Vivian O. Sammons. (Washington, D.C.: Hemisphere Publishers), 1989. p.158.
Encyclopedia of Black America. Agustus Low and Virgil A. Clift, editors. (New York, NY: McGraw-Hill), 1981. p. 744.
Who's Who in the East. 17th edition, 1979-1980. (Wilmette, IL: Marquis Who's Who), 1979.
Who's Who in the East. 16th edition, 1977-1978. (Wilmette, IL: Marquis Who's Who), 1977.
Hope this helps,
Ellery
--------------- Ellery E. Frahm Research Scientist/Manager Electron Microprobe Laboratory Department of Geology & Geophysics University of Minnesota - Twin Cities Lab Website: http://probelab.geo.umn.edu
On 19 Feb 2004, by way of Ask-A-Microscopist wrote: } } Email: JAHMIT-at-aol.com } Name: Natalie Heltman } } Organization: E.H. Greene } } Education: K-8 Grade Grammar School } } Location: Cincinnati, OH 45242 } } Question: Hello, I'm doing a research paper on John William } Coleman and I cannot find any information on him. } I was in need of any info. you can provide. I know that he was a member of } Electron Microscopy } Society? What was his role in the development of the electron microscope with } RCA Labs? } } Any info. would help I'm sending this message form my Dad's work place. } Thanks, Natalie
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 14:50:02 2004
You may want to contact SRI [Sarnoff Research International] in Princeton, New Jersey. This was formerly Sarnoff Research, a division of RCA Corp. which I am an alumni of.
Peter Tomic Agere Systems, formerly Lucent Technologies, Att etc.
-----Original Message----- } From: by way of Ask-A-Microscopist [mailto:JAHMIT-at-aol.com] Sent: Thursday, February 19, 2004 2:57 PM To: microscopy-at-ns.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (JAHMIT-at-aol.com) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Thursday, February 19, 2004 at 13:41:24 ---------------------------------------------------------------------------
Email: JAHMIT-at-aol.com Name: Natalie Heltman
Organization: E.H. Greene
Education: K-8 Grade Grammar School
Location: Cincinnati, OH 45242
Question: Hello, I'm doing a research paper on John William Coleman and I cannot find any information on him. I was in need of any info. you can provide. I know that he was a member of Electron Microscopy Society? What was his role in the development of the electron microscope with RCA Labs?
Any info. would help I'm sending this message form my Dad's work place. Thanks, Natalie
Does anyone have an extra 3-1/4 x 4" plate holder insert that they wouldn't mind getting rid of? We only need the insert (from a two-piece holder) from a Philips EM 410. I believe the design hasn't changed in many years and that it is the same for any CM or 400 series. I can email a jpg image to anyone who might want to compare what I have with what they have.
Thank you in advance,
Doug
---------------------- Douglas R. Keene Associate Investigator Micro-Imaging Center Shriners Hospital for Children 3101 S.W. Sam Jackson Park Road Portland, Oregon 97239-3009 phone: 503-221-3434 FAX: 503-412-6894
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 15:27:56 2004
On Feb 19, 2004, at 6:43 AM, by way of MicroscopyListserver wrote:
} Name: Jessica deryk } } Title-Subject: [Microscopy] [Filtered] MListserver:Confusing } calculations } } Question: Hi, } I was hoping someone could help me find, what I thought would be } fundamental relationships for microscopy but are proving to be } difficult to determine. } I have a LeicaMz16a steromicroscope with; } 7.11x to 155x mag } FOV range: 29.5mm to 1.82mm } } This is coupled to a CCD camera with; } 1300x1030 pixel array } pixel spacing: 6.7microns } FOW: 8.7 x 6.9 mm } } I'm wondering how I can accurately calculate the effective FOV as seen } by the rectangular CCD. Also the relationship between magnification in } the microscope and resulting pixel spacing of my digital images?? } } I would appreciate any advice in these calculations or references to } texts which may contain information regarding interaction between CCD } cameras and microscopes. } Dear Jess, If the FOV of the magnified image (mFOV) was the same at all mags, then mag x mFOV = FOV. Since that is not true for the two mags you list, there must be an additional constraint within the scope that does not let you see as big an area at low mag; that could be a source of math confusion. In contrast, the size of the FOV of the CCD is consistent with the number of pixels and the pixel size, and that size is smaller than the FOVs for each mag, so the CCD should see a rectangular area within the FOV at any mag. The FOV on the object that can be seen by the CCD is just the CCD area divided by the mag, so for the two mags listed, the CCD FOVs would be 1.22 mm x 0.97 mm for 7.11x and 0.056 mm x 0.044 mm for 155x. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 15:35:40 2004
We have a Balzer's 301 Freeze Fracture Apparatus available to anyone who can take it away. The system was modified by Wiltek and equipped with a digital vacuum control system, a Mass Spec and Cryopump (there are two cryopumps both currently non-operational). A brief description and pictures will be available at http://www.cscn.com/gsis/prod01.htm . If you're interested please contact me, Richard Harris by phone 519-661-2111 ext 86780 or e-mail rjharris-at-uwo.ca. Thanks
Richard Harris Laboratory Supervisor Department of Biology University of Western Ontario London Ontario CANADA Ph. 519-661-2111 ext. 86780 Fax 519-661-3935
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 15:47:30 2004
VLSI Standards, Inc. Strategic Accounts Manager 3087 North First Street San Jose, CA 95134-2006 Phone: (408) 428-1800, ext. 108 Fax: (408) 428-9555 E-mail: marc.helvey-at-vlsistd.com Internet: http://www.vlsistandards.com
-----Original Message----- } From: JAHMIT-at-aol.com [mailto:JAHMIT-at-aol.com] Sent: Thursday, February 19, 2004 11:57 AM To: microscopy-at-ns.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (JAHMIT-at-aol.com) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Thursday, February 19, 2004 at 13:41:24 ---------------------------------------------------------------------------
Email: JAHMIT-at-aol.com Name: Natalie Heltman
Organization: E.H. Greene
Education: K-8 Grade Grammar School
Location: Cincinnati, OH 45242
Question: Hello, I'm doing a research paper on John William Coleman and I cannot find any information on him. I was in need of any info. you can provide. I know that he was a member of Electron Microscopy Society? What was his role in the development of the electron microscope with RCA Labs?
Any info. would help I'm sending this message form my Dad's work place. Thanks, Natalie
Hi Diane I know nothing about "colloidal carbon" - does it exist in this world? From another hand I very doubt that even "colloidal carbon" could help you. I suppose you want to see the fine details of your sample if were trying to use negative staining. DNA is 2.4-3 nm in diameter, even naked DNA may not be visualized well using negative staining. You may see DNA using "positive staining" but it's quite difficult and you need to use high-res. STEM and probably Z-contrast (and NEVER Formvar support film!!!!!). To see any DNA on polymer's background is a big problem because your polymer will scatter most electrons... Trying to see one polymer (yours) on another polymer (Formvar) is seems to me even more challenged. I would suggest, you need to try freeze-fracture in combination with good SEM. It will give you idea of 3D structure of polymer and you probably will be able to see some DNA conglomerates (not DNA strands). Sometime DNA created sort of periodic structure, which you also could see. I know that some people froze polymer and do ultrathin sections from it, stain with OsO4 or UA and analyzed it under TEM. Good luck, Sergey.
P.S. In general, you may not use any plastic support film for high resolution TEM. You need to use thin carbon support film for precise work.
At 09:27 AM 2/19/2004, you wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry 10833 Le Conte Ave, Room 33-089 Los Angeles, CA 90095
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi Diane I know nothing about "colloidal carbon" - does it exist in this world? From another hand I very doubt that even "colloidal carbon" could help you. I suppose you want to see the fine details of your sample if were trying to use negative staining. DNA is 2.4-3 nm in diameter, even naked DNA may not be visualized well using negative staining. You may see DNA using "positive staining" but it's quite difficult and you need to use high-res. STEM and probably Z-contrast (and NEVER Formvar support film!!!!!). To see any DNA on polymer's background is a big problem because your polymer will scatter most electrons... Trying to see one polymer (yours) on another polymer (Formvar) is seems to me even more challenged. I would suggest, you need to try freeze-fracture in combination with good SEM. It will give you idea of 3D structure of polymer and you probably will be able to see some DNA conglomerates (not DNA strands). Sometime DNA created sort of periodic structure, which you also could see. I know that some people froze polymer and do ultrathin sections from it, stain with OsO4 or UA and analyzed it under TEM. Good luck, Sergey.
P.S. In general, you may not use any plastic support film for high resolution TEM. You need to use thin carbon support film for precise work.
At 09:27 AM 2/19/2004, you wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry 10833 Le Conte Ave, Room 33-089 Los Angeles, CA 90095
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi Diane I know nothing about "colloidal carbon" - does it exist in this world? From another hand I very doubt that even "colloidal carbon" could help you. I suppose you want to see the fine details of your sample if were trying to use negative staining. DNA is 2.4-3 nm in diameter, even naked DNA may not be visualized well using negative staining. You may see DNA using "positive staining" but it's quite difficult and you need to use high-res. STEM and probably Z-contrast (and NEVER Formvar support film!!!!!). To see any DNA on polymer's background is a big problem because your polymer will scatter most electrons... Trying to see one polymer (yours) on another polymer (Formvar) is seems to me even more challenged. I would suggest, you need to try freeze-fracture in combination with good SEM. It will give you idea of 3D structure of polymer and you probably will be able to see some DNA conglomerates (not DNA strands). Sometime DNA created sort of periodic structure, which you also could see. I know that some people froze polymer and do ultrathin sections from it, stain with OsO4 or UA and analyzed it under TEM. Good luck, Sergey.
P.S. In general, you may not use any plastic support film for high resolution TEM. You need to use thin carbon support film for precise work.
At 09:27 AM 2/19/2004, you wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry 10833 Le Conte Ave, Room 33-089 Los Angeles, CA 90095
Dear Chris, If no one else has given you a perchloric acid-free electrolyte for Ti alloys, look in "Metallography Principles and Practices" by Vander Voort. He lists several electrolytes for Ti and Ti alloys in Appendix H that do not contain perchloric acid. If you cannot find a Vander Voort book (which you should definitely have before you start electropolishing Ti), write back to me and I will give you a couple of recipes out of my copy. I have no experience with using them myself. Regards, Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchange.ubc.ca ----- Original Message ----- } From: "by way of MicroscopyListServer" {cmeyer911-at-yahoo.com} To: "MicroscopyListserver" {microscopy-at-msa.microscopy.com} Sent: Wednesday, February 11, 2004 2:23 PM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (richard-at-polymer.kth.se) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, February 19, 2004 at 15:44:28 ---------------------------------------------------------------------------
Email: richard-at-polymer.kth.se Name: Richard Olsson
Organization: Royal Institute of technology
Title-Subject: [Microscopy] [Filtered] MListserver: Epoxy matrix for nanoparticles
Question: Dear listeners, Can anyone suggest a good curing agent for a regular Bisphenol-A- epichlorohydrin resin (Epon 828 or sililair). I am using magnetic ferrite nanoparticles (20-100nm) and I want to lock them in their positions very quickly after dispersing them with an ultrasonic probe in the epoxy. At the moment I am playing with viscosity to keep them separated in the "pure epoxy" but I also want to cure my epoxy and shorten the curing time to as short as possible so that I can keep them separated in the cured sample.
Thank you all for listening.
Regards from Sweden Richard Olsson
Ps. I wish to avoid to high exotherms because my sample is 30 millimeters thick and I want to avoid degradation of my matrix.
} Date: Thu, 19 Feb 2004 13:04:49 -0800 } To: Julie.Glasscock-at-csiro.au (by way of MicroscopyListserver) } From: Gary Gaugler {gary-at-gaugler.com} } Subject: [Microscopy] Re: viaWWW: Faraday cup } } To make a specimen current reading, the stage } must be isolated such that the Faraday cup } is electrically brought out of the chamber. } The BNC connector should do this. However, if } the BNC connector is not currently shorted via } a shorting plug, chances are that the stage is } not isolated. If it were isolated and not } grounded, the specimens would charge up. You can } verify continuity by measuring resistance from } the stage (specimen holder) to door frame ground-- } this should read infinity (no continuity). Then } read from stage (holder) to center pin of BNC--this } should read zero Ohms (closed circuit). } } If the above checks out, you need a Faraday cup. } If there is not one in your system, you can buy } one from SEM supply houses like MSA, et. al. or } make one. A Faraday cup is a hole in a piece of } metal or Carbon with a small hole at the top. The } easiest way to do this is to mount a 100u aperture } on top of a pin stub that has a hole drilled in it. } } Then take a DVM and plug it into the BNC connector. } Put the DVM on the lowest voltage range and read the } voltage. Current will be voltage divided by 10^6. } I=E/R where I is the current, E is the voltage reading } and R is the ten meg Ohms input resistance of the DVM. } The jazzy way to do this is to use a picoampmeter. } But these cost about $1,000 vs. about $100 for a DVM. } } gary g. } } } At 06:43 AM 2/19/2004, you wrote: } } } } ------------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
From MicroscopyL-request-at-ns.microscopy.com Thu Feb 19 22:06:54 2004
Looks like there was a spam attack while I was on my way back from across the Pacific. Unfortunately, the long flight mean't I was off-line for nearly 2 days and a number (but not all) of you appeared to have been hit by a forged from address during the last 2 days.
Please remember any Email that does not have the phrase
as the begining of the SUBJECT line did not go through the Listserver Email filters. You should immediately suspect that message as junk mail, even if it appears to come from "MICROSCOPY.COM".
I think I have found and plugged the hole that could have allowed the junk mail to get to some of you. However, remember the spoofing/faking of Email addresses now abounds and it is very difficult to track.
Don't be afraid to report suspect Email . I try to look into everything, to try to minimize problems.
My apologies...
Nestor Your Friendly Neighborhood SysOp
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 20 00:56:48 2004
Nestor, I think we all know how problematic SPAM gets and how difficult it is to get rid of it. To be honnest, I'm already surprised that not more gets through, I guess you must already block a lot of messages! For those who are still receiving SPAM-messages, it might be interesting to install an extra SPAM-filter on your email, so it gets double-checked and will decrease the number even more. Just look around the net, there are a few very good, cheap or even free anti-SPAM-programs available. We have one here on at the university and still I get about 15 mails per day on average which were not blocked. Those senders are really abusing their knowledge I think! Good luck & best regards, Sven
-----Original Message----- } From: Nestor J. Zaluzec [mailto:zaluzec-at-microscopy.com] Sent: vrijdag 20 februari 2004 05:17 To: microscopy-at-ns.microscopy.com
Colleagues
Looks like there was a spam attack while I was on my way back from across the Pacific. Unfortunately, the long flight mean't I was off-line for nearly 2 days and a number (but not all) of you appeared to have been hit by a forged from address during the last 2 days.
Please remember any Email that does not have the phrase
as the begining of the SUBJECT line did not go through the Listserver Email filters. You should immediately suspect that message as junk mail, even if it appears to come from "MICROSCOPY.COM".
I think I have found and plugged the hole that could have allowed the junk mail to get to some of you. However, remember the spoofing/faking of Email addresses now abounds and it is very difficult to track.
Don't be afraid to report suspect Email . I try to look into everything, to try to minimize problems.
My apologies...
Nestor Your Friendly Neighborhood SysOp
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 20 10:38:18 2004
I had asked the list a while ago regards to replacement lamps for a Microtek 2500F scanner.
A number of folks asked for the outcome, and I also feel it is my responsibility to inform the scientific community of serious issues with one of the top recommended scanners.
It seems that for whatever reason microtek will NOT sell or ship replacement lamps for this scanner. YOU MUST ship the scanner back to microtek ITSELF. There are no authorized repair facilities (in North America) other than the single office center in California. Now please folks realize, we are talking about shiiping a 70 pound, delicate scanner that costs ~ $3K USD, and that comes in a box as big as a desk across a continent. For a lamp assembly that is replaced with 6 screws total, and should have a cost of less than $20.
For anyone out there who has replaced a flat bed scanner lamp you know exactly how absurd this is.
Please, I urge you DO NOT BUY MICROTEK SCANNERS.
(Anyone want to buy a Scanner? Needs a new lamp.)
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 350 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 20 10:47:00 2004
I apologize for this double posting made an error in the Web page address - use the address in this e-mail thank you.
We have a Balzer's 301 Freeze Fracture Apparatus available to anyone who can take it away. The system was modified by Wiltek and equipped with a digital vacuum control system, a Mass Spec and Cryopump (there are two cryopumps both currently non-operational). A brief description and pictures will be available at http://www.gsisinc.com If you're interested please contact me, Richard Harris by phone 519-661-2111 ext 86780 or e-mail rjharris-at-uwo.ca. Thanks
Richard Harris Laboratory Supervisor Department of Biology University of Western Ontario London Ontario CANADA Ph. 519-661-2111 ext. 86780 Fax 519-661-3935
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 20 10:56:02 2004
Has anyone come up with a way to QC these grids "on the fly"? We've started using them for IEM and have had a few that weren't totally coated with Au; the Cu etched in my Tris buffer (yes, I know I can use phosphate instead). Sometimes it is obvious, but I came in this morning to find 4 blue antibody drops....I was sure these 4 grids were well-coated.
Any ideas? Current thinking is to give up and use these as expensive Cu grids and go back to annoying Ni for IEM (plain Au is a bit pricey and the students tend to turn them into tacos).
Thanks!
Tamara
|--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward-at-unm.edu |--------------------------------------------------|
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 20 11:36:46 2004
Have you considered procuring a replacement lamp from another source based on the identification on the lamp itself? Chances are that Microtek doesn't make it anyway, they buy it from someone else. It's often the case that the same replacement part is marked up 100% by the equipment manufacturer if they have to stock it as a replacement item. You might contact Aristo, Gilway Technical Lamp, BulbDirect.com or similar. It might be less trouble than packing and shipping the scanner anywhere and cheaper as well.
John Twilley
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 20 12:20:26 2004
Try Pureland Supply as well: www.purelandsupply.com Randy
-----Original Message----- } From: John Twilley [mailto:jtwilley-at-sprynet.com] Sent: Friday, February 20, 2004 12:13 PM To: edelmare-at-MUOHIO.EDU Cc: microscopy-at-msa.microscopy.com
Tony,
XEI Scientific makes the Evactron Anti-contaminator for removing oil from SEM Chambers and X-ray windows. Full details about the Evactron system can be found at our web site: Evactron.com.
We have just submitted two abstracts to M&M 2004 meeting about this subject: "A Study of the Effects of Evactron® Plasma Cleaning on X-ray Windows" by R.Vane, C.Roberts, and V.L. Carlino and "Improved Carbon Analysis with Evactron Plasma Cleaning" by Pierre Rolland, Vince Carlino, and Ronald Vane
We would be happy to send you preprints. Please e-mail Sales-at-Evactron.com and request X-ray papers.
Ronald Vane XEI Scientific
----- Original Message ----- } From: "by way of MicroscopyListserver" {tgreco-at-seas.marine.usf.edu} To: {microscopy-at-ns.microscopy.com} Sent: Thursday, February 19, 2004 10:56 AM
} -Diane-
DNA won't stick to formvar. One must use colloidin which is (I think) some form of nitrocellulose. It is also barely possible to visualize it by staining. The 1.5 nm diameter limits this technique, not because it is below the resolving power of the EM, but because one cannot get enough stain on the double helix to distinguish it from background by conventional imaging techniques.
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-- __ Carol A. Heckman, Ph.D. Professor of Biological Sciences Director, Center for Microscopy & Microanalysis Bowling Green State University, Bowling Green, OH 43403 fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu ___________________________________________________________________________
From MicroscopyL-request-at-ns.microscopy.com Fri Feb 20 14:56:40 2004
Jessica, I guess I will address your question even though you are a Hawkeye.
I would do way with most of the numbers your gave. I would roll most everything together into a black box and start from scratch. There is usually a variety of unknown optics in the path so that the magnification/field of view/pixel spacing is not a straightforward calculation. There is also the issue that the CCD size is not always well-matched to the microscope so that the field of view at the camera is typically not quite the same as through the eyepieces. I simply take an object of known size and take images of it at various magnifications and calculate the desired parameters. You could then do a follow-up exercise to calculate the cumulative effect of the intermediate parts.
Warren, a Cyclone
At 08:43 AM 2/19/2004, you wrote: } ------------------------------------------------------------------------------- } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (jderyk-at-dpi.radiology.uiowa.edu) from } http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on } Wednesday, February 18, 2004 at 15:32:49 } --------------------------------------------------------------------------- } } Email: jderyk-at-dpi.radiology.uiowa.edu } Name: Jessica deryk } } Title-Subject: [Microscopy] [Filtered] MListserver:Confusing calculations } } Question: Hi, } I was hoping someone could help me find, what I thought would be } fundamental relationships for microscopy but are proving to be difficult } to determine. } I have a LeicaMz16a steromicroscope with; } 7.11x to 155x mag } FOV range: 29.5mm to 1.82mm } } This is coupled to a CCD camera with; } 1300x1030 pixel array } pixel spacing: 6.7microns } FOW: 8.7 x 6.9 mm } } I'm wondering how I can accurately calculate the effective FOV as seen by } the rectangular CCD. Also the relationship between magnification in the } microscope and resulting pixel spacing of my digital images?? } } I would appreciate any advice in these calculations or references to texts } which may contain information regarding interaction between CCD cameras } and microscopes. } Thanks } Jess
------------------------------------------- No files should be attached to this message ------------------------------------------- Warren E. Straszheim, Ph.D. Materials Analysis and Research Lab Iowa State University 46 Town Engineering Ames IA, 50011-3232
We have a Hitachi S2460N that we have been leaving at 40 Pa when idle (nights and weekends). It has remained clean for almost 10 years now. Our JEOL 840A could benefit from an XEI system. Since it remains at high vacuum, we build up oil contamination on the EDS detector as it acts as a cold finger.
I have been told that some time at 40 Pa would remove contamination after it built up. I do not know. I suppose it could work, but I don't think the vapor pressure of the pump oil would be so high as to make it a very fast process. I think a system such as XEI's which uses an activated species would do better at removing accumulated contamination. Of course, you still might want to CAREFULLY clean your dirty x-ray detector first.
You say that even your chamber is dirty. That sounds like an extreme problem to me. We accumulate oil on the x-ray detector, but I have yet to see oily surface elsewhere inside our scopes. Maybe there is some other problem with the vacuum system that a service engineer should examine.
Warren
At 12:56 PM 2/19/2004, you wrote:
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------------------------------------------- No files should be attached to this message ------------------------------------------- Warren E. Straszheim, Ph.D. Materials Analysis and Research Lab Iowa State University 46 Town Engineering Ames IA, 50011-3232
I know how to make a composite DAPI, FITC, Rhodamine (i.e., Blue, Green, Red) color image by pasting the grey scale digital fluorescence images into the RGB channels but how do you get a 4th color (e.g., Far Red 647) into the image. I know there must be a simple way that my tired old mind is forgetting. Could some Photoshop maven help me out? Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 3 Tucker Hall University of Missouri Columbia, MO 65211-7400
Sorry, Tom, you can't do that. Human eyes have three kinds of cones, and consequently computer displays have three kinds of phosphors. You can generate a wide range of color combinations with those three phosphors, and so there is no fourth color available that doesn't occur when the right proportions of the three are present. Now, if you were a pigeon, you'd have 5 kinds of cones, and you would have designed monitors with more phosphors, and been able to handle more independent signals at the same time.
John Russ ======== In a message dated 2/20/04 6:19:40 PM, phillipst-at-missouri.edu writes:
} I know how to make a composite DAPI, FITC, Rhodamine (i.e., Blue, Green, } } Red) color image by pasting the grey scale digital fluorescence images } into } the RGB channels but how do you get a 4th color (e.g., Far Red 647) into } } the image. I know there must be a simple way that my tired old mind is } } forgetting. Could some Photoshop maven help me out? Thanks, Tom
From MicroscopyL-request-at-ns.microscopy.com Sat Feb 21 07:08:53 2004
} I know how to make a composite DAPI, FITC, Rhodamine (i.e., } Blue, Green, Red) color image by pasting the grey scale } digital fluorescence images into the RGB channels but how } do you get a 4th color (e.g., Far Red 647) into the image. } ...
Making a composite given R,G & B is straightforward, i.e., all channels contribute equally to the presentation (if not the aesthetics). Possibilities for adding a 4th channel are possible, but now you are confronted with non-equal contributions to the presentation, and with what channels, for what influence. An example would be to create a CMYK file and put the grayscales into the 4 channels as you would with 3 into RGB ... but which channel do you put into the 'K' channel, which might do nothing, or something strange, with what you're trying to present.
Another possibility is to pick 3 for the RGB channels, and put a yellow layer on top, and use the 4th channel as a "layer mask". Again it depends on what you want this 4th channel to present, but this method offers more flexibility (e.g., use different color layers ... use the mask as 'show' or 'hide' modes ... and you can "blend" the additional layer in any number of ways, e.g., difference, multiply, etc)
Alas ... in the end, what have you got? Does the image present well? Will your colleagues understand the presentation? I have this nagging feeling you're asking "what does everyone else do?" towards creating a presentation everyone can redily understand. I am not familiar with these channels of information, but the last possibility is to blend 2 channels into 1, and your colleagues may need to come to agreement on which channels. For example, if the channels were elemental spatial maps, you may blend Fe and Mg because they substitute for each other (usually). At my wwwsite, there is an example of where I blended Na & Ca for the same reason (plagioclase feldspar).
This is certainly an interesting problem. Of course, the astronomers deal with similar ones all the time, as they try to assign different non-visible wavelengths to color images. I would think that you might be able to assign different combinations of color to each channel instead of limiting yourself to RGB. One way to think of this might be to construct specific color look-up-tables for each of the gray scale images using a program like ImageJ so that each channel would have its own color structure. Then, you could convert each of the images to RGB (which would calculate the ratios automatically) and use Photoshop to overlay one on the other. Areas of colocalization will appear in interesting combinations, but the unique areas should show through in the colors that you have assigned.
Of course John is right that there are only 3 types of color receptors in the human eye, and the same number of different phosphors in a CRT, but I disagree with John that you cannot have more than 3 colors for different fluorochromes. Unfortunately I cannot speak for Photoshop, as I don't know enough about that software, but we do that routinely in our mFIP module (Multiple Fluorescence Image Processing). Instead of assigning just red, green, and blue, you can also assign other colors, such as, for example, yellow or magenta and thus create color images with more than 3 fluorochromes.
I don't know how you would do this in Photoshop, but you could, for example, assign only red to one fluorochrome, green to another, blue to the third, and blue and red in equal proportions to the fourth.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: DrJohnRuss-at-aol.com [mailto:DrJohnRuss-at-aol.com] Sent: Friday, February 20, 2004 17:08 To: phillipst-at-missouri.edu; Microscopy-at-msa.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (R.H.Olley-at-reading.ac.uk) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, February 21, 2004 at 10:28:29 ---------------------------------------------------------------------------
Email: R.H.Olley-at-reading.ac.uk Name: Robert H. Olley
Question: We are finding that computer controlled 'experiments' do not inspire our undergraduates. Does anyone know of suitable physics experiments for students that would use an optical microscope?
Of course, you can assign another signal to the yellow channel, or the magenta channel, but it cannot show uniquely that signal, because the colocalization of red and green will produce an indistinguishable yellow from the one you've assigned to the additional channel, etc. Doing it in Photoshop is simple - just convert the RGB image to CMYK and paste into the C M or Y channel (don't mess with the black channel - you will get some unexpected and unwelcome effects when you go back to RGB). The problem is that the color channels have become garbled and may not communicate the intended information. It is usually better to try to overlay textures on regions to try to illustrate more signal channels. Or, as someone else has already suggested, try to create derived combinations of your "pure channel" signals to identify structural regions of interest. PCA is a good way to pursue getting the three "best" combinations of signals to put into RGB. To learn more, check out the (rich) literature on satellite imagery, where the problems of more than 3 channels are old news.
John Russ ===== In a message dated 2/21/04 11:20:34 AM, mb-at-soft-imaging.com writes:
} Of course John is right that there are only 3 types of color receptors } in } the human eye, and the same number of different phosphors in a CRT, but } I } disagree with John that you cannot have more than 3 colors for different } fluorochromes. Unfortunately I cannot speak for Photoshop, as I don't know } enough about that software, but we do that routinely in our mFIP module } (Multiple Fluorescence Image Processing). Instead of assigning just red, } green, and blue, you can also assign other colors, such as, for example, } yellow or magenta and thus create color images with more than 3 } fluorochromes. } } I don't know how you would do this in Photoshop, but you could, for example, } assign only red to one fluorochrome, green to another, blue to the third, } and blue and red in equal proportions to the fourth. }
From MicroscopyL-request-at-ns.microscopy.com Sat Feb 21 12:25:51 2004
These pumps were made by Edwards Vacuum for Philips, as a special order. EP-100, 100 l/s. I doubt you can get a brand new replacement. You may, however, rebuild this pump at Duniway Stockroom, they do a good job. (650)969-8811 or www.duniway.com
Vitaly Feingold Scientific Instruments and Applications 2773 Heath Lane, Duluth GA 30096 (770)232-7785 ph. (770)232-1791 fax (678)467-0012 mobile www.sia-cam.com
This message is made of 100% recycled electrons.
This address can not receive messages larger than 15 kb without prior notification.
----- Original Message ----- } From: "Hank Beebe (by way of MicroscopyListServer)" {hbeebe-at-rjlg.com} To: "MicroscopyListserver" {microscopy-at-msa.microscopy.com} Sent: Saturday, February 14, 2004 8:03 PM
Robert,
Many years ago, one could use uranium block glass to demonstrate the exit pupil of the condenser lens, thus showing the affects of changing substage condenser aperture in brightfield, the hollow cone of darkfield and Rheinberg, and single azimuth or oblique angle illumination. Furthermore, students could visually see the affect of changing for instance, the aperture or condenser height and how these parameters related to numerical aperture.
However, I do not have access to block glass. Therefore, I use a single sheet of white notecard stock, approximately 4cm x 4cm, folded 90 degrees. One portion of the card rests on the stage while the other portion extends upward toward the objective. You will need to lower your stage or raise your objective to fit the card into position. The card is then moved just into position so as to reveal the illumination of the exit pupil and cast in effect the angular aperture angle on the card.
This is a very simple demonstration and one can 'experiment' with the condenser optics to understand the relationship of angular aperture, working distance, and numerical aperture.
I have other suggestions, but this is one of the most simple and fundamentally important. Good Luck!
Sincerely, Ken
_______________________________________ Kenneth L. Tiekotter, Adjunct Professor The University of Portland Department of Biology 5000 N Willamette Blvd. Portland, OR 97203 USA
Director, MicroImaging Dx Center Legacy Portland Hospitals Legacy Holladay Park Medical Center 1225 NE 2nd Avenue Portland, OR 97232 USA
Tel.: 503.413.5391
On 2/21/04 8:50 AM, "by way of MicroscopyListserver" {R.H.Olley-at-reading.ac.uk} wrote:
} } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------} - } } Below is the result of your feedback form (NJZFM-ultra-55). It was submitted } by (R.H.Olley-at-reading.ac.uk) from } http://microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, } February 21, 2004 at 10:28:29 } --------------------------------------------------------------------------- } } Email: R.H.Olley-at-reading.ac.uk } Name: Robert H. Olley } } Organization: University of Reading } } Title-Subject: [Microscopy] [Filtered] MListserver: OM: Phyiscs Experiments } } Question: We are finding that computer controlled 'experiments' do not inspire } our undergraduates. Does anyone know of suitable physics experiments for } students that would use an optical microscope? } } } --------------------------------------------------------------------------- }
From MicroscopyL-request-at-ns.microscopy.com Sat Feb 21 12:58:54 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jcma01-at-students.stir.ac.uk) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Saturday, February 21, 2004 at 12:35:51 ---------------------------------------------------------------------------
Email: jcma01-at-students.stir.ac.uk Name: Jafet
Organization: University of Stirling
Education: Undergraduate College
Location: Stirling, Scotland, Great Britaion
Question: Hi!
I'm a relatively new ligth microscope user. I've been working with a compound microscope full time for a week now. After a few hours of use, my eyes (especially one eye) get sore and eventually I get a headace. Is this normal? Can prolonged use lead to eye damage? Can anything be done to avoid it?
I continue to have problems getting uranyl acetate into solution (aqueous or acetone) so that it not only goes in but stays in for a reasonable period of time (weeks preferably). Concentrations can vary from 0.5% to 2% but the problem remains. I have tried uranyl acetate from a number of different sources and still have minimal luck.
What we do now is put the required amount into the solvent and then let stir on a magnetic stirrer, often for hours. Then we filter out what does not dissolve. Of course this leaves an unknown concentration in the final solution.
Does anyone have a brand to recommend that dissolves well or any special tricks?
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 03:22:06 2004
Dear Debby Uranyl Acetate is quite soluble in the water. 1-2% (w/v) aqueous solutions are stable in the dark at +4oC for a few months. Usually I dissolve UA in plastic tube with gentle shaking. It's completely dissolved in about 40 min at RT. If your "uranyl acetate" is not dissolved in water, it means, it's not UA from chemical point of view. Perhaps, it's uranyl nitrate, which is less dissolvable in water... I would think EVERY EM supplier will be happy to supply you with fresh real uranyl acetate which SHOULD be dissolvable in the water by Merck Index... Sergey
At 01:25 PM 2/22/2004 -0500, you wrote:
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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 07:10:10 2004
You'll want to make sure that both eyepieces are adjusted correctly. That is, one or both will have an adjustment that can compensate for the difference in vision between the eyes. If these are significantly out of focus you may have the symptoms you describe. It also helps to keep lenses and mirrors clean. In the end, see your eye care physician to make sure you don't have any issues with your eyes themselves.
Hope that's of some help.
Peter
-----Original Message----- } From: by way of Ask-A-Microscopist [mailto:jcma01-at-students.stir.ac.uk] Sent: Saturday, February 21, 2004 2:09 PM To: microscopy-at-ns.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jcma01-at-students.stir.ac.uk) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Saturday, February 21, 2004 at 12:35:51 ---------------------------------------------------------------------------
Email: jcma01-at-students.stir.ac.uk Name: Jafet
Organization: University of Stirling
Education: Undergraduate College
Location: Stirling, Scotland, Great Britaion
Question: Hi!
I'm a relatively new ligth microscope user. I've been working with a compound microscope full time for a week now. After a few hours of use, my eyes (especially one eye) get sore and eventually I get a headace. Is this normal? Can prolonged use lead to eye damage? Can anything be done to avoid it?
As it was explained to me, (I'm blind in one eye so cannot say that it works, but it should) -
One of the two eyepieces on the compound microscope should have a focusing ring on it. The other should not. Look with one eye through the eyepiece that does not, and focus it with the microscope's focusing knob(s).
Look with the other eye through the eyepiece that has its own focusing ring and, use only that ring to focus the image in that eye. Finally, adjust the eyepieces sideways to match your interpupilar distance by moving the eyepieces either closer together or further apart.
Ron L
-----Original Message----- } From: Tomic, Peter (Peter) [mailto:ptomic-at-agere.com] Jafet;
You'll want to make sure that both eyepieces are adjusted correctly. That is, one or both will have an adjustment that can compensate for the difference in vision between the eyes. If these are significantly out of focus you may have the symptoms you describe. It also helps to keep lenses and mirrors clean. In the end, see your eye care physician to make sure you don't have any issues with your eyes themselves.
Hope that's of some help.
Peter
-----Original Message----- } From: by way of Ask-A-Microscopist [mailto:jcma01-at-students.stir.ac.uk]
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jcma01-at-students.stir.ac.uk) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Saturday, February 21, 2004 at 12:35:51 ---------------------------------------------------------------------------
Email: jcma01-at-students.stir.ac.uk Name: Jafet
Organization: University of Stirling
Education: Undergraduate College
Location: Stirling, Scotland, Great Britaion
Question: Hi!
I'm a relatively new ligth microscope user. I've been working with a compound microscope full time for a week now. After a few hours of use, my eyes (especially one eye) get sore and eventually I get a headace. Is this normal? Can prolonged use lead to eye damage? Can anything be done to avoid it?
} You'll want to make sure that both eyepieces are adjusted } correctly. That is, one or both will have an adjustment that can } compensate for the difference in vision between the eyes. If } these are significantly out of focus you may have the symptoms } you describe. It also helps to keep lenses and mirrors clean. } In the end, see your eye care physician to make sure you don't } have any issues with your eyes themselves.
I would only add, ... my experience with my users having eye strain problems usually finds that they haven't yet found a way to relax and focus their eyes at infinity ... and then make the ocular and eyepiece adjustments. I've also not yet found a way for them to take a systematic approach (suggestions?) ... but what can help is to gaze out the window at infinity for a short period, and then switch to the m'scope and make adjustments while similarly relaxed.
} I'm a relatively new ligth microscope user. I've been working } with a compound microscope full time for a week now. After a few } hours of use, my eyes (especially one eye) get sore and } eventually I get a headace. Is this normal? Can prolonged use } lead to eye damage? Can anything be done to avoid it?
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 07:57:03 2004
Sergey, I am using uranyl acetate not uranyl nitrate. At least I believe the label on the bottles. I have gotten it from leading supply houses recently and also have some older bottles from a variety of supply houses. This is not a new problem...one I have had over many years. Apparently from the response to my E-mail lots of others have the same problem.
Keeping the solution dark does help slow down precipitation but it still occurs and this does not help with the initial dissolving of the reagent.
One solution seems to be to add acetic acid to the water to lower pH. The pH of water, although usually acidic, does vary depending on the purification method. Does anyone know the optimum pH for dissolving UA? This solution however will not work with acetone when you want to add UA for freeze substitution.
Perhaps we need to forget the percentages listed in all the methods and just admit that we are using "saturated" solutions of UA or report the pH just like you do with other solutions.
Debby
On 2/23/04 4:40 AM, "Sergey Ryazantsev" {sryazant-at-ucla.edu} wrote:
} } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------} - } } Dear Debby } Uranyl Acetate is quite soluble in the water. 1-2% (w/v) aqueous solutions } are stable in the dark at +4oC for a few months. Usually I dissolve UA in } plastic tube with gentle shaking. It's completely dissolved in about 40 } min at RT. If your "uranyl acetate" is not dissolved in water, it means, } it's not UA from chemical point of view. Perhaps, it's uranyl nitrate, } which is less dissolvable in water... I would think EVERY EM supplier will } be happy to supply you with fresh real uranyl acetate which SHOULD be } dissolvable in the water by Merck Index... Sergey } } At 01:25 PM 2/22/2004 -0500, you wrote: } } } } -----------------------------------------------------------------------------} } - } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.msa.microscopy.com/MicroscopyListserver } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------------- } } -- } } } } Hi folks, } } } } I continue to have problems getting uranyl acetate into solution } } (aqueous or acetone) so that it not only goes in but stays in for a } } reasonable period of time (weeks preferably). Concentrations can vary from } } 0.5% to 2% but the problem remains. I have tried uranyl acetate from a } } number of different sources and still have minimal luck. } } } } What we do now is put the required amount into the solvent and then let } } stir on a magnetic stirrer, often for hours. Then we filter out what does } } not dissolve. Of course this leaves an unknown concentration in the final } } solution. } } } } Does anyone have a brand to recommend that dissolves well or any special } } tricks? } } } } Debby } } } } Debby Sherman, Manager Phone: 765-494-6666 } } Life Science Microscopy Facility FAX: 765-494-5896 } } Purdue University E-mail: dsherman-at-purdue.edu } } S-052 Whistler Building } } 170 S. University Street } } West Lafayette, IN 47907 } } http://www3.agriculture.purdue.edu/microscopy } } }
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 07:58:49 2004
As it was explained to me, (I'm blind in one eye so cannot say that it works, but it should) -
One of the two eyepieces on the compound microscope should have a focusing ring on it. The other should not. Look with one eye through the eyepiece that does not, and focus it with the microscope's focusing knob(s).
Look with the other eye through the eyepiece that has its own focusing ring and, use only that ring to focus the image in that eye. Finally, adjust the eyepieces sideways to match your interpupilar distance by moving the eyepieces either closer together or further apart.
Ron L
-----Original Message----- } From: Tomic, Peter (Peter) [mailto:ptomic-at-agere.com] Jafet;
You'll want to make sure that both eyepieces are adjusted correctly. That is, one or both will have an adjustment that can compensate for the difference in vision between the eyes. If these are significantly out of focus you may have the symptoms you describe. It also helps to keep lenses and mirrors clean. In the end, see your eye care physician to make sure you don't have any issues with your eyes themselves.
Hope that's of some help.
Peter
-----Original Message----- } From: by way of Ask-A-Microscopist [mailto:jcma01-at-students.stir.ac.uk]
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jcma01-at-students.stir.ac.uk) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Saturday, February 21, 2004 at 12:35:51 ---------------------------------------------------------------------------
Email: jcma01-at-students.stir.ac.uk Name: Jafet
Organization: University of Stirling
Education: Undergraduate College
Location: Stirling, Scotland, Great Britaion
Question: Hi!
I'm a relatively new ligth microscope user. I've been working with a compound microscope full time for a week now. After a few hours of use, my eyes (especially one eye) get sore and eventually I get a headace. Is this normal? Can prolonged use lead to eye damage? Can anything be done to avoid it?
We have always used a saturated soltn of uranyl acetate in water for staining grids; when we make it up, we stir overnight, then once it settles, we use the "clear" stain. For use, we dilute the saturated UA 1:1 with 100% methanol and then filter through a 0.4 micron syringe filter into the Hiroaka staining trough. We have never had bad results.
Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Laboratories of Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
-----Original Message----- } From: Debby Sherman [mailto:dsherman-at-purdue.edu] Sent: Monday, February 23, 2004 9:11 AM To: Sergey Ryazantsev; message to: MSA list
Sergey, I am using uranyl acetate not uranyl nitrate. At least I believe the label on the bottles. I have gotten it from leading supply houses recently and also have some older bottles from a variety of supply houses. This is not a new problem...one I have had over many years. Apparently from the response to my E-mail lots of others have the same problem.
Keeping the solution dark does help slow down precipitation but it still occurs and this does not help with the initial dissolving of the reagent.
One solution seems to be to add acetic acid to the water to lower pH. The pH of water, although usually acidic, does vary depending on the purification method. Does anyone know the optimum pH for dissolving UA? This solution however will not work with acetone when you want to add UA for freeze substitution.
Perhaps we need to forget the percentages listed in all the methods and just admit that we are using "saturated" solutions of UA or report the pH just like you do with other solutions.
Debby
On 2/23/04 4:40 AM, "Sergey Ryazantsev" {sryazant-at-ucla.edu} wrote:
} } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------} - } } Dear Debby } Uranyl Acetate is quite soluble in the water. 1-2% (w/v) aqueous solutions } are stable in the dark at +4oC for a few months. Usually I dissolve UA in } plastic tube with gentle shaking. It's completely dissolved in about 40 } min at RT. If your "uranyl acetate" is not dissolved in water, it means, } it's not UA from chemical point of view. Perhaps, it's uranyl nitrate, } which is less dissolvable in water... I would think EVERY EM supplier will } be happy to supply you with fresh real uranyl acetate which SHOULD be } dissolvable in the water by Merck Index... Sergey } } At 01:25 PM 2/22/2004 -0500, you wrote: } } } } -----------------------------------------------------------------------------} } - } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.msa.microscopy.com/MicroscopyListserver } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------------- } } -- } } } } Hi folks, } } } } I continue to have problems getting uranyl acetate into solution } } (aqueous or acetone) so that it not only goes in but stays in for a } } reasonable period of time (weeks preferably). Concentrations can vary from } } 0.5% to 2% but the problem remains. I have tried uranyl acetate from a } } number of different sources and still have minimal luck. } } } } What we do now is put the required amount into the solvent and then let } } stir on a magnetic stirrer, often for hours. Then we filter out what does } } not dissolve. Of course this leaves an unknown concentration in the final } } solution. } } } } Does anyone have a brand to recommend that dissolves well or any special } } tricks? } } } } Debby } } } } Debby Sherman, Manager Phone: 765-494-6666 } } Life Science Microscopy Facility FAX: 765-494-5896 } } Purdue University E-mail: dsherman-at-purdue.edu } } S-052 Whistler Building } } 170 S. University Street } } West Lafayette, IN 47907 } } http://www3.agriculture.purdue.edu/microscopy } } }
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 09:57:26 2004
} I would only add, ... my experience with my users having eye strain problems } usually finds that they haven't yet found a way to relax and focus their } eyes at infinity ... and then make the ocular and eyepiece adjustments. } I've also not yet found a way for them to take a systematic approach } (suggestions?)
Having in the beginnig the same problem, I found very much help in an old booklet from Zeiss, were they wrote : "don't look IN the microscope, but TROUGH the microscope"
It's only psychology, but it helps. You must look "at the object" and not "in the microscope", and when you have forgotten the microscope, it's OK. It's the same thing looking a deer or a rabbit trough glasses.
Jacques Faerber IPCMS
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 10:02:04 2004
Members in the Greater Baltimore-Washingon, D.C. area are cordially invited to "Cryoultramicrotomy Techniques for The Biomedical and Biological Sciences."
There is no charge for this workshop, and refreshments will be served!
When: Thursday, March 4, 9:30am - 5:00pm Friday, March 5, 9:30am - 2:00pm
Where: Johns Hopkins University Medical School Ross Bldg., #529 720 Rutland Baltimore, MD 21205
What: A two-day "mini-workshop" with lectures, presentations, demonstrations and hands-on sessions for cryoultramicrotomy and associated techniques (cryo tool making, glass knife making, evaluation of glass knives, care and cleaning of diamond knives, operation of the ultramicrotome and immunoelectron microscopy).
Important Info: Attendance is open (but space is limited) for all of the presentations and demonstrations on Thursday, March 4th. The lecture room can accommodate approximately 15 people.
Also due to space requirements, attendance is limited to 12 people for the cryoultramicrotomy hands-on sessions on Friday, March 5th.
Contact: For additional details, to RSVP or to reserve a spot for the hands-on sessions, please contact Kim Megaw at Boeckeler Instruments, Inc. ( {Kim-at-boeckeler.com} , 800.552.2262).
Sponsors and Organizers: RMC Products Group, Boeckeler Instruments, Inc.
Dr. David Ryugo's Laboratory Department of Neuroscience Johns Hopkins School of Medicine
Hope to see you there!
Bob Chiovetti Boeckeler Instruments, Inc.
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 13:54:13 2004
I am in the process of repairing a JEOL JSM T200 SEM. One of the scan circuits in the electron optic system is not operational. The problem seems to be related to the "Operation Board".
The PK00023 EF version of the Operation Board schematics I have on hand do not quite match the the circuit board that is installed in the SEM, they are a different revision level. I have contacted JEOL and they no longer seem to have the one I need.
I am looking for a schematic for the PH00023 EF version of the Operation Board.
Any assistance or direction, will be much appreciated.
Best Regards,
Grant Baumgardner
Arizona State University Center for High Resolution Microscopy
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 15:00:11 2004
Dear Debby Yes, I think it's a water problem. I usually use 20 Mohm/cm2 "cell culture" grade water from Milli-Q (Millipore). pH of water depends from CO2 presence and are not characteristic. Usually, at the normal circumstances, water exposures to the air has pH 5-5.5. Basic water pH usually indicates germ contamination (on the filter, lines etc). In Russia we used to use double-distilled in quartz water without any problems. According Merck Index, UA is soluble in 10 parts of water, which means 10%. Try Ted Pella UA Cat # 19481 - it works fine to me - no special preference is given to Ted Pella. The bottom line, perhaps, (I never think about it) water should be acidic, not basic. Sergey
At 06:10 AM 2/23/2004, you wrote: } Sergey, } I am using uranyl acetate not uranyl nitrate. At least I believe the } label on the bottles. I have gotten it from leading supply houses recently } and also have some older bottles from a variety of supply houses. This is } not a new problem...one I have had over many years. Apparently from the } response to my E-mail lots of others have the same problem. } } Keeping the solution dark does help slow down precipitation but it still } occurs and this does not help with the initial dissolving of the reagent. } } One solution seems to be to add acetic acid to the water to lower pH. The pH } of water, although usually acidic, does vary depending on the purification } method. Does anyone know the optimum pH for dissolving UA? This solution } however will not work with acetone when you want to add UA for freeze } substitution. } } Perhaps we need to forget the percentages listed in all the methods and } just admit that we are using "saturated" solutions of UA or report the pH } just like you do with other solutions. } } Debby } } } } On 2/23/04 4:40 AM, "Sergey Ryazantsev" {sryazant-at-ucla.edu} wrote: } } } } } } } } ------------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.msa.microscopy.com/MicroscopyListserver } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------------} } - } } } } Dear Debby } } Uranyl Acetate is quite soluble in the water. 1-2% (w/v) aqueous solutions } } are stable in the dark at +4oC for a few months. Usually I dissolve UA in } } plastic tube with gentle shaking. It's completely dissolved in about 40 } } min at RT. If your "uranyl acetate" is not dissolved in water, it means, } } it's not UA from chemical point of view. Perhaps, it's uranyl nitrate, } } which is less dissolvable in water... I would think EVERY EM supplier will } } be happy to supply you with fresh real uranyl acetate which SHOULD be } } dissolvable in the water by Merck Index... Sergey } } } } At 01:25 PM 2/22/2004 -0500, you wrote: } } } } } } } } -----------------------------------------------------------------------------} } } - } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } } http://www.msa.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } ----------------------------------------------------------------------------- } } } -- } } } } } } Hi folks, } } } } } } I continue to have problems getting uranyl acetate into solution } } } (aqueous or acetone) so that it not only goes in but stays in for a } } } reasonable period of time (weeks preferably). Concentrations can vary } from } } } 0.5% to 2% but the problem remains. I have tried uranyl acetate from a } } } number of different sources and still have minimal luck. } } } } } } What we do now is put the required amount into the solvent and } then let } } } stir on a magnetic stirrer, often for hours. Then we filter out what does } } } not dissolve. Of course this leaves an unknown concentration in the final } } } solution. } } } } } } Does anyone have a brand to recommend that dissolves well or any special } } } tricks? } } } } } } Debby } } } } } } Debby Sherman, Manager Phone: 765-494-6666 } } } Life Science Microscopy Facility FAX: 765-494-5896 } } } Purdue University E-mail: dsherman-at-purdue.edu } } } S-052 Whistler Building } } } 170 S. University Street } } } West Lafayette, IN 47907 } } } http://www3.agriculture.purdue.edu/microscopy } } } } } }
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry 10833 Le Conte Ave, Room 33-089 Los Angeles, CA 90095
In the UA that is commercially available (radioactively "depleted", but probably irrelevant to this discussion on solubility) there is always a small amount of totally insoluable (in water) "contaminant" - that according to my vendor whom I discussed this with about 5 years ago.
So when I mix up 3% UA in distilled water, I stir it on magnetic mixer for an hour, add 1 drop of concentrated glacial acetic acid per 10.0 ml of stain to reduce long term U ppt formation (it works, I've kept small vials of stain from same batch with and without the GAA and is less ppt in the GAA treated stain), then let it stand overnight, and carefully pipet off the clear UA into a clean, clear glass bottle, store inside a dark box. It will stay clear with no ppt gathering on the bottom of the bottle for about 1-2 months, then maybe a real fine layer may be discerned on the bottle bottom, at which point we filter it through 0.2 micron filters as we use it.
By the way, I collected some of that insoluble component that settled out during the night after dissolving the UA, washed those crystals with distilled water to get off any residual UA, and did EDS on them in my SEM/EDS machine. All crystals examined had high to medium amounts of titanium, Silicon and uranium in them, medium amounts of oxygen, low amounts of iron and aluminum, some with low phosphorous. So the crystals are probably a mix of 2-3 types of an insoluable uranium compound.
As for ending up with unknown concentration from filtering, you're probably still pretty close to the 2% target you use, and if you mix up same way/amount each time and stain for some empirically determined time, at least you'll be consistent.
In sum, there will always be some insoluable crystals left when dissolving UA, so handle as above to minimize or eliminate ppt from that source on sections.
Gib -- Gib Ahlstrand, Scientist Electron Optical Facility, University of Minnesota, CBS Imaging Center, 35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454 (612)624-2785 FAX, ahlst007-at-tc.umn.edu http://www.cbs.umn.edu/ic/
"You can learn a lot by observation - just by lookin'!" - Yogi Berra
} Hi folks, } } I continue to have problems getting uranyl acetate into solution } (aqueous or acetone) so that it not only goes in but stays in for a } reasonable period of time (weeks preferably). Concentrations can vary from } 0.5% to 2% but the problem remains. I have tried uranyl acetate from a } number of different sources and still have minimal luck. } } What we do now is put the required amount into the solvent and then let } stir on a magnetic stirrer, often for hours. Then we filter out what does } not dissolve. Of course this leaves an unknown concentration in the final } solution. } } Does anyone have a brand to recommend that dissolves well or any special } tricks? } } Debby } } Debby Sherman, Manager Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www3.agriculture.purdue.edu/microscopy
From MicroscopyL-request-at-ns.microscopy.com Mon Feb 23 15:31:16 2004
I think, the problem here what you want from the final image. If you just want to have "map" with sort of color table for each stain (like one area is green, another -pink etc), in this case you could not owerlap areas. Or you need to represent amount of stain (let say brighter red means more your rhodamine stain)- if so, you may not introduce other than RGB channels as John Russ absolutely correctly pointed out. If you will introduce other than RGB colors - you may not quantitate anymore because yellow may be your "4th color" or just superposition of "red" and "green" from other stains... the same for any other colors because all of them are superposition of RGB by definition. I think, more scientific way to represent such data is to present gray (or real color) images for all your stains and show separately superposition of RGB for 3 stains, so you need more than one superimposed color picture for more than 3 stains. It's also make sence, because in real you have red, green and blue fluorescence. Sergey
At 08:02 AM 2/21/2004, you wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry 10833 Le Conte Ave, Room 33-089 Los Angeles, CA 90095
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Grant.Baumgardner-at-asu.edu) from on Monday, February 23, 2004 at 11:12:14 ---------------------------------------------------------------------------
Email: Grant.Baumgardner-at-asu.edu Name: Grant Baumgardner
Organization: Arizona State University Center for High Resolution Microscopy
Title-Subject: [Microscopy] [Filtered] MListserver: Correct Schematics for JEOL JSM T200
Question: Dear Fellow ListServer Users:
I am in the process of repairing a JEOL JSM T200 SEM. One of the scan circuits in the electron optic system is not operational. The problem seems to be related to the "Operation Board".
The PK00023 EF version of the Operation Board schematics I have on hand do not quite match the the circuit board that is installed in the SEM, they are a different revision level. I have contacted JEOL and they no longer seem to have the one I need.
I am looking for a schematic for the PH00023 EF version of the Operation Board.
Any assistance or direction, will be much appreciated.
Best Regards,
Grant Baumgardner
Arizona State University Center for High Resolution Microscopy
All arc lamps require a warmup period. 40 min is about the minimum. How long do you leave the burner running? They do not like being switched off and on a few times a day. It leads to a short life.
New burners require a burn in period before they are stable, IIRC it is a period of several hours.
I haven't seen a comparison study of stability between mercury and Xenon arc lamps. I do know that the DC mercury burners produce a more stable arc with less flicker than the AC varieties.
Neither is an inexpensive upgrade, usually requiring a new power supply, socket and maybe a new lamp house as well to obtain the proper optics for the new bulb.
Bob
On 12 Feb 2004, at 19:23, Marie-Claude Bélanger wrote: } } Dear all, } } I have a couple questions regarding the illumination stability of mercury } burners: } } 1- When a new burner is installed, is there a period of time (within a few } hours) where the intensity increases to a plateau? With an older lamp, } everytime we turn the lamp on, we have observed that it takes about 40 minutes } to reach a plateau. We installed a new burner recently, and images have been } brighter from day to day for the first 3 days and remained stable afterwards. } Is it just a coincidence? } } 2- To solve the stability problems we have with the mercury burners, we’re } considering the use of xenon lamps. Is there somewhere a comparative stability } study of the 2 types of burners? } } Thank you. } } } } Marie-Claude Belanger } } _________________________________________________________________ } MSN Messenger : discutez en direct avec vos amis ! } http://messenger.fr.msn.ca/ }
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 09:06:45 2004
As an addendum, I received another information from Sylvania-Osram, stating that the burn in period for a new lamp is 24 hours.
Marie-Claude Belanger
On 23 Feb 2004, at 21:03, Bob Sunley wrote:
} } All arc lamps require a warmup period. 40 min is about the minimum. } How long do you leave the burner running? They do not like being switched } off and on a few times a day. It leads to a short life. } } New burners require a burn in period before they are stable, IIRC it is a } period of several hours. } } I haven't seen a comparison study of stability between mercury and Xenon } arc lamps. I do know that the DC mercury burners produce a more stable } arc with less flicker than the AC varieties. } } Neither is an inexpensive upgrade, usually requiring a new power supply, } socket and maybe a new lamp house as well to obtain the proper optics for } the new bulb. } } Bob } } On 12 Feb 2004, at 19:23, Marie-Claude Bélanger wrote: } } } } Dear all, } } } } I have a couple questions regarding the illumination stability of } mercury } } burners: } } } } 1- When a new burner is installed, is there a period of time (within a } few } } hours) where the intensity increases to a plateau? With an older lamp, } } everytime we turn the lamp on, we have observed that it takes about 40 } minutes } } to reach a plateau. We installed a new burner recently, and images have } been } } brighter from day to day for the first 3 days and remained stable } afterwards. } } Is it just a coincidence? } } } } 2- To solve the stability problems we have with the mercury burners, } we’re } } considering the use of xenon lamps. Is there somewhere a comparative } stability } } study of the 2 types of burners? } } } } Thank you. } } } } } } } } Marie-Claude Belanger } } } } _________________________________________________________________ } } MSN Messenger : discutez en direct avec vos amis ! } } http://messenger.fr.msn.ca/ } } } }
_________________________________________________________________ MSN Search, le moteur de recherche qui pense comme vous ! http://fr.ca.search.msn.com/
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 10:28:39 2004
} I think I have found and plugged the hole that could have allowed the } junk mail to get to some of you. However, remember } the spoofing/faking of Email addresses now abounds and } it is very difficult to track. } } Don't be afraid to report suspect Email . I try to } look into everything, to try to minimize problems. } } } My apologies... } } Nestor } Your Friendly Neighborhood SysOp } }
Gail S. Kenner Department of Botany University of British Columbia 3529-6270 University Blvd. Vancouver, British Columbia V6T 1Z4 Canada
tel: (604) 822-5223 FAX: (604) 822-6089
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 12:38:32 2004
I searching for information on the Olympus Video archiving system (VAS) II. I have one, and I have been asked to determine if we can network the system to down load images to other work stations. There are ports on the back of the unit, but I can find no reference to what they are for, nor can I find any software for the darn thing. Any help would be appreciated!!
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 14:14:31 2004
A colleague asked for any information on the following:
Plastic coverslips. They used to be made by a company called Oncor Cat # S1370-14. The company doesn't make them anymore and web searches for them using google only turns up the thermanox coverlips. If anyone knows anything about them, such as what type of plastic it is or any other plastic companies that make something like this.
If any one knows any thing about this product she would be most grateful. I will pass along any information that anyone has.
Thanks.
ML
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 14:39:51 2004
Hi, We used the Thermonox coverslips since the 70's for SEM preps of tissue cultures, bacteria, bacterial spores, etc. At least then, the coverslips withstood the solutions normally used for SEM i.e. G-Os,EtOH, then either CPD or Freeze Drying. They certainly withstood metal coatings and evaporator heat as well. Hope that is helpful.
Judy M.
Judy Murphy, PhD San Joaquin Delta College Microscopy Technology 5151 Pacific Ave Stockton, CA 95207 209-954-5284 FAX 209-954-5600 jmurphy-at-deltacollege.edu http://www.sjdccd.cc.ca.us/dept/electmicro/index.html
Mei Lie Wong wrote: } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } A colleague asked for any information on the following: } } Plastic coverslips. They used to be made by a company } called Oncor } Cat # S1370-14. The company doesn't make them anymore and } web searches for them using google only turns up the } thermanox } coverlips. If anyone knows anything about them, such as } what type of plastic it is or any other plastic companies } that make something like this. } } If any one knows any thing about this product she would be } most grateful. I will pass along any information that } anyone has. } } Thanks. } } ML
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 14:43:04 2004
Try Fisher Scientific's unbreakable cover slips (catolog number 12-547). I don't know if they will meet your needs, but I use them for making impressions of hair scales and they work quite nicely.
Best wishes.........
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 15:12:22 2004
Dear colleagues, how do you archive pictures taken by digital camera? There is some problems with images and data stored on the CD.
Keep care and be of good cheer
Regards
(name) Vratislav Richard Eugene Maria John Baptist (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
Tenebrionidae of the World, incl. Alleculinae and Lagriinae, higher taxonomy, Australian beetles. websites: http://www.coleoptera.org. and http://www.egroups.com/group/coleoptera
University of Sydney The Wentworth Bldg., B 62 NSW 2006 AUSTRALIA phone : +61 414 540 465 email: ricardo-at-ans.com.au vratislav-at-bigfoot.com ICQ: 13610107
Only after the last tree has been cut down, only after the last river has been poisoned, only after the last fish has been caught, only then will you find that money can not be eaten.' CREE INDIAN PROPHECY.
Incoming mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com).
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 17:19:29 2004
There can be problems archiving files to cheap CDs. Same for DVDs. A good approach is to use Mitsui CDs and DVDs. Never had a problem. A bit more expensive but well worth the non-loss of precious data. Be sure that your burning software performs a verify after burn.
gary g.
At 01:27 PM 2/24/2004, you wrote:
} Dear colleagues, } how do you archive pictures taken by digital camera? } There is some problems with images and data stored on the CD. } } Keep care and be of good cheer } } Regards } } (name) Vratislav Richard Eugene Maria John Baptist } (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 21:14:30 2004
As a computer network admin type, I can't emphasize enough to burn 3 copies on good media. CDR's are not really archival media yet. They do last a good while, but they are easily damaged and they do die a natural death.
Bob
On 24 Feb 2004, at 15:32, Gary Gaugler wrote:
} } There can be problems archiving files to cheap } CDs. Same for DVDs. A good approach is to use } Mitsui CDs and DVDs. Never had a problem. A } bit more expensive but well worth the non-loss } of precious data. Be sure that your burning } software performs a verify after burn. } } gary g. } } } At 01:27 PM 2/24/2004, you wrote: } } } Dear colleagues, } } how do you archive pictures taken by digital camera? } } There is some problems with images and data stored on the CD. } } } } Keep care and be of good cheer } } } } Regards } } } } (name) Vratislav Richard Eugene Maria John Baptist } } (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld } }
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 21:46:33 2004
This topic has been discussed before. The bottom line is to backup to quality CD/DVD media and to redundantly backup to tape. I use Ultrium 2 LTO. Yes, the CDs and DVDs are duplicated (just 2) but only one LTO tape. I have never lost a file. However, having said that, I do keep copies on Dell NAS servers.
gary g.
At 07:28 PM 2/24/2004, you wrote:
} As a computer network admin type, I can't emphasize enough to burn 3 } copies on good media. CDR's are not really archival media yet. They do } last a good while, but they are easily damaged and they do die a natural } death. } } Bob } } } On 24 Feb 2004, at 15:32, Gary Gaugler wrote: } } } } } There can be problems archiving files to cheap } } CDs. Same for DVDs. A good approach is to use } } Mitsui CDs and DVDs. Never had a problem. A } } bit more expensive but well worth the non-loss } } of precious data. Be sure that your burning } } software performs a verify after burn. } } } } gary g. } } } } } } At 01:27 PM 2/24/2004, you wrote: } } } } } Dear colleagues, } } } how do you archive pictures taken by digital camera? } } } There is some problems with images and data stored on the CD. } } } } } } Keep care and be of good cheer } } } } } } Regards } } } } } } (name) Vratislav Richard Eugene Maria John Baptist } } } (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld } } } }
From MicroscopyL-request-at-ns.microscopy.com Tue Feb 24 23:46:48 2004
-- [ From: Garber, Charles A. * EMC.Ver #3.1a ] --
Vladimir Dusevich wrote: ================================================================ What is a good embedding media for bone ultramicrotomy? I use Epon 812 and Spurr resin, but penetration is not really good enough. ===============================================================\ I would refer you to the publications of M. Cole, a few of which are listed below:
Cole, M. B., Jr., Gylcol methacrylate embedding of bone and cartilage for light microscopic staining, J. Microsc. (Oxf), 127, 139-148 (1982).
This was taken from a longer list of publications on URL http://www.2spi.com/catalog/chem/rep-references.html
The SPI-Chem™ Brand Low Acid GMA is based on the work of Dr. Cole. See URL http://www.2spi.com/catalog/chem/embed4.shtml
The GMA monomer is perhaps the most "infiltrateable" resin of all, since it has a viscosity slightly less than that of water. Another advantage of the GMA system is that dehydration is not necessary since some water is needed to make the polymerization occur. It has been used widely on both bone, cartilage, and teeth.
Chuck
Disclaimer: SPI Supplies is the supplier of the SPI-Chem brand Low Acid GMA
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 05:05:01 2004
Vladimir Dusevich wrote: ================================================================ What is a good embedding media for bone ultramicrotomy? I use Epon 812 and Spurr resin, but penetration is not really good enough.
I asked my colleque Saddha Cuijpers and she wrote me this answer:
Working with archaeological bone and especially with cremated remains the embedding agent Biodur E12 in combination with E1 (Dr. G. von Hagens, Heidelberg) works very good and is easy to apply. This two-component resin infiltrates unburnt bone without trouble and gives a good clear histological picture under the microscope. When dealing with burnt bone fragments, however, a cover glass is glued on the top before cutting to keep this material together, because cremated bone is not easily infiltrated. Reference: Herrmann, B., G. Grupe, S. Hummel, H. Piepenbrink & H. Schutkowski (1990). Prähistorische Anthropologie. Berlin: Springer Verlag, pp. 186-207. van der Lubbe e.a., 1988. A simple method fo preparing thin histological sections of undecalcified plastic embedded bone with implants.in Stain Technology 63, 171-176.
Ineke
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 08:08:40 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 25, 2004 at 07:15:27 ---------------------------------------------------------------------------
Email: mccaulak-at-wfu.edu Name: Anita McCauley
Organization: Wake Forest University
Title-Subject: [Microscopy] [Filtered] MListserver: capture board for an SEM
Question: I am looking to upgrade the image capture board for our Amray 1810 SEM. I am trying to find a board that will capture NTSC composite video at 640x480 pixels using TWAIN or WDM capture and is compatible with Windows XP. We have found a couple of possibilities: Flashpoint 4XL Lite, Flashbus Spectrum Lite framegrabber, Data Translation DT3120.
Any suggestions for boards that would work or any feedback regarding the boards I mentioned would be greatly appreciated.
Thanks
Anita K. McCauley, PhD Director of Microscopy Adjunct Asst. Professor Biology Department Wake Forest University Winston-Salem, NC 27109
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tgardiner-at-mmtinc.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 25, 2004 at 08:21:13 ---------------------------------------------------------------------------
The draft program of the international conference Focus on Microscopy 2004 is now available, please review under http://www.focusonmicroscopy.org . Abstracts for the poster session may be still submitted, but may not be included in the printed conference material.
Plenary speakers include:
S. Hell: Fluorescence nanoscopy through reversible optically saturable transitions Z. Galis: Exploring revascularization using fluorescence angiography: going with the flow S.M. Hewitt: Confocal imaging meets tissue microarrays: high throughput biology moves to the next level E. Manders, Controlled light exposure microscopy (clem): an effective reduction of phototoxicity. R.F. Murphy, R.F.: Automated interpretation of images for location proteomics B. Parvin: Convergence of microscopy and imaging bioinformatics A. Periasamy: Protein localization in cells and tissues: Flim-Fret microscopy K. Pourezzaei:Application of nano optical tools to single cell imaging A. Waggoner: Quantum dots for in-vivo imaging in mice W. Zipfel: Applications of multiphoton microscopy and nonlinear excitation
Sessions are dedicated to the following topics: Optical theory, multidimensional image processing, 3D/4D in-vivo imaging, non-linear biological imaging, Raman/Cars microscopy, tuning the microscope resolution, image restoration, new instrumentation, high-througput microscopy and human cytome project
Also included: Pre-conference workshops, trade show, poster session
Looking forward seeing you in Philadelphia,
Andres Kriete, Philadelphia Fred Brakenhoff, Amsterdam -- +-----------------------------------------+ Prof. Dr. G.J. Brakenhoff University of Amsterdam Institute for Molecular Cell Biology Section Molecular Cytology Kruislaan 316 1098 SM Amsterdam, The Netherlands
You can display as many colors as you like, with the caveat that you won't be able to separate them again if they're mixed.
In our software (Image-Pro, using the Colro Composite) we allow compositing as many colors as desired; red, green, blue, yellow, cyan, magenta, etc. We do this by assigning each input a particular color mix - an input image assigned to yellow contributes equally to red and green, while orange contributes fully to red and only half as much to green.
With three colors (RGB) you can separate them out from each other using only the final 24-bit color composite. With multiple color mixing using combinations of RGB you cannot. However, you can view these just fine as long as the objects of different colors do not overlap (and mix) too much. We even allow white, so that you can overlay DIC and fluorescence images; this works quite well if you treat the 'white' input as a background to the others.
-- Kevin Ryan kryan-at-mediacy.com
Media Cybernetics, Inc. www.mediacy.com
-----Original Message----- } From: DrJohnRuss-at-aol.com [mailto:DrJohnRuss-at-aol.com] Sent: Friday, February 20, 2004 17:08 To: phillipst-at-missouri.edu; Microscopy-at-msa.microscopy.com
Sorry, Tom, you can't do that. Human eyes have three kinds of cones, and
consequently computer displays have three kinds of phosphors. You can generate a wide range of color combinations with those three phosphors, and so there is no fourth color available that doesn't occur when the right proportions of the three are present. Now, if you were a pigeon, you'd have 5 kinds of cones, and you would have designed monitors with more phosphors, and been able to handle more independent signals at the same time.
John Russ ======== In a message dated 2/20/04 6:19:40 PM, phillipst-at-missouri.edu writes:
} I know how to make a composite DAPI, FITC, Rhodamine (i.e., Blue, } Green, } } Red) color image by pasting the grey scale digital fluorescence images } into the RGB channels but how do you get a 4th color (e.g., Far Red } 647) into } } the image. I know there must be a simple way that my tired old mind is } } forgetting. Could some Photoshop maven help me out? Thanks, Tom
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 09:25:00 2004
During the past month, I have been working on semithick sectioning. The sections are around green-purple , which translates into thickness of 120-250 micron. To spread the wrinkles , I used Chloroform and it worked fine. However, it made me very sick, shortness of breath. I am wondering if any of you have better and safer methods to spread the thick sections.
Thank you,
Long
----------------------------------------------- Miao, Long Dept of Biological Science 334 Bio. Unit1 Florida State University Tallahassee, FL32306-4370 email: lmiao-at-bio.fsu.edu Voice: (850)644-9817 FAX : (850)644-0481 -----------------------------------------------
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 10:06:45 2004
The March 8th meeting of the New England Society for Microscopy (NESM) will be held at Worcester Polytechnic Institute (WPI), Worcester, MA. Three speakers, two from the WPI faculty and one from UMASS will present a "Forum on Nanotechnology".
Registration is from 5-6pm followed by a reception/dinner from 5:30-7pm. The registration fee for this meeting is $25.00 (members and non-members alike). Please visit NESM's website (MSA website-local societies) for further details. Click on "current newsletter" for directions, a map, and registration form. To register, print out the registration form, and send completed form with money to Paul Bain, NESM Treasurer by March 5, 2004.
We hope many of you will attend this most informative meeting!
Peggy Sherwood Corresponding Secretary, NESM
Peggy Sherwood Lab Associate, Photopathology Wellman Laboratories of Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 10:08:31 2004
I bought a heat pen from Ted Pella (I have no financial interests in this company). It was expensive ($300??) but a lot better than the small battery operated units (~$40). I liked it so much, I bought two more for my other ultramicrotomes. We use it on both thin and semi-thin. It is a lot better and safer than chloroform (long term liver problems can result from repeated inhalation). good luck
10:37 AM 2/25/2004 -0500, you wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 3 Tucker Hall University of Missouri Columbia, MO 65211-7400
You may try this at Acton Technologies, http://www.actontech.com/elec3.htm
Peter Tomic Agere Systems
-----Original Message----- } From: by way of MicroscopyListserver [mailto:tgardiner-at-mmtinc.com] Sent: Wednesday, February 25, 2004 9:46 AM To: microscopy-at-ns.microscopy.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tgardiner-at-mmtinc.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 25, 2004 at 08:21:13 ---------------------------------------------------------------------------
Have you heard of a heat pen? Theycan be used to get rid of wrinkles. You hold it over the sections like you do the chloroform stick and the wrinkles should go away. I don't know which EM vendor has them, but it used to be tha all of the EM vendors sold them.
Good luck and please get some fresh air,
Paula :-)
Paula Sicurello George Washington Univ. Medical Center Electron Microscope Lab Washington, DC 20037 202-994-2930 phone 202-994-2518 fax } } } "Long Miao" {lmiao-at-bio.fsu.edu} 02/25/04 10:51 AM } } }
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Dear list,
During the past month, I have been working on semithick sectioning. The sections are around green-purple , which translates into thickness of 120-250 micron. To spread the wrinkles , I used Chloroform and it worked fine. However, it made me very sick, shortness of breath. I am wondering if any of you have better and safer methods to spread the thick sections.
Thank you,
Long
----------------------------------------------- Miao, Long Dept of Biological Science 334 Bio. Unit1 Florida State University Tallahassee, FL32306-4370 email: lmiao-at-bio.fsu.edu Voice: (850)644-9817 FAX : (850)644-0481 -----------------------------------------------
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 10:21:53 2004
We are having problems with our Hitachi S-570 and a service technician is not available until the end of April. Perhaps someone has experienced a similar problem and can give us some advice.
At low magnifications, everything appears to be okay (perhaps because the irregularity is too small to see) but around 2500 X the entire image on the CRT begins to wave. This waviness increases in size when the magnification is increased. For instance, if a straight line is focused on, a regular frequency wave appears to travel along the line. Magnify this image and the wave also magnifies. The problem is accentuated when moving from aperture 3 to aperture 2.
We don't believe any other equipment is causing an external disturbance. We have changed the filament, cleaned the Wehnelt, anode, and movable and fixed apertures. Any ideas on a possible solution?
Shannan
Shannan Little Research Technician/Technicien de recherche Electron Microscopy and Image Analysis / Microscopie électronique et Analyse d'images Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 403-317-3446 Facsimile/Télécopieur: 403-382-3156 P.O. Box 3000 / CP 3000 Lethbridge, Alberta T1J 4B1 littlesm-at-em.agr.ca http://res2.agr.ca/lethbridge/emia/index_e.htm
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 10:34:22 2004
stop using chloroform immediately. It's harmful and should have been risk assessed - not only is it an anaesthetic it is harmful over a prolonged exposure by inhalation and a category 3 carcinogen. We only use chloroform in the fume hood these days.
The safest method is to buy a heat pen - available at most e.m suppliers. This uses a hot tungsten wire to soften and spread your sections.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear UK
----- Original Message ----- } From: Long Miao {lmiao-at-bio.fsu.edu}
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Focus on Microscopy 2004, Philadelphia, April 4-8
Dear Colleagues,
The draft program of the international conference Focus on Microscopy 2004 is now available, please review under http://www.focusonmicroscopy.org . Abstracts for the poster session may be still submitted, but may not be included in the printed conference material.
Plenary speakers include:
S. Hell: Fluorescence nanoscopy through reversible optically saturable transitions Z. Galis: Exploring revascularization using fluorescence angiography: going with the flow S.M. Hewitt: Confocal imaging meets tissue microarrays: high throughput biology moves to the next level E. Manders, Controlled light exposure microscopy (clem): an effective reduction of phototoxicity. R.F. Murphy, R.F.: Automated interpretation of images for location proteomics B. Parvin: Convergence of microscopy and imaging bioinformatics A. Periasamy: Protein localization in cells and tissues: Flim-Fret microscopy K. Pourezzaei:Application of nano optical tools to single cell imaging A. Waggoner: Quantum dots for in-vivo imaging in mice W. Zipfel: Applications of multiphoton microscopy and nonlinear excitation
Sessions are dedicated to the following topics: Optical theory, multidimensional image processing, 3D/4D in-vivo imaging, non-linear biological imaging, Raman/Cars microscopy, tuning the microscope resolution, image restoration, new instrumentation, high-througput microscopy and human cytome project
Also included: Pre-conference workshops, trade show, poster session
Looking forward seeing you in Philadelphia,
Andres Kriete, Philadelphia Fred Brakenhoff, Amsterdam -- +-----------------------------------------+ Prof. Dr. G.J. Brakenhoff University of Amsterdam Institute for Molecular Cell Biology Section Molecular Cytology Kruislaan 316 1098 SM Amsterdam, The Netherlands
The solutions for immersion etching are typically a mixture of high concentrations of strong acids which can be nasty to use and don't store well.
Electrolytic etching is the preferred method. Start with 2% H2SO4 in water, 3-10 V dc, 5-15 secs with Pt lead wires. The sufuric acid concentration may be increased up to 20% for a deeper etch.
Stu Smalinskas Metallurgist SKF NATC Plymouth, Michigan
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Tammy wrote:
Question: Does anyone know how to etch 80% Nickle 20% Chrome to see the microstructure.
Email: tgardiner-at-mmtinc.com Name: Tammy
__________________________________ Do you Yahoo!? Yahoo! Finance: Get your refund fast by filing online. http://taxes.yahoo.com/filing.html
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 11:39:44 2004
Instead of a $300 heat pen how about a soldering iron/gun?
Geoff
Tom Phillips wrote:
} ------------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } } I bought a heat pen from Ted Pella (I have no financial interests in } this company). It was expensive ($300??) but a lot better than the } small battery operated units (~$40). I liked it so much, I bought two } more for my other ultramicrotomes. We use it on both thin and } semi-thin. It is a lot better and safer than chloroform (long term } liver problems can result from repeated inhalation). good luck } } } 10:37 AM 2/25/2004 -0500, you wrote: } } } } ------------------------------------------------------------------------------ } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.msa.microscopy.com/MicroscopyListserver } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------------- } } } } } } Dear list, } } } } During the past month, I have been working on semithick sectioning. The } } sections are around green-purple , which translates into thickness of } } 120-250 micron. To spread the wrinkles , I used Chloroform and it } } worked } } fine. However, it made me very sick, shortness of breath. I am } } wondering } } if any of you have better and safer methods to spread the thick } } sections. } } } } Thank you, } } } } Long } } } } } } ----------------------------------------------- } } Miao, Long } } Dept of Biological Science } } 334 Bio. Unit1 } } Florida State University } } Tallahassee, FL32306-4370 } } email: lmiao-at-bio.fsu.edu } } Voice: (850)644-9817 } } FAX : (850)644-0481 } } ----------------------------------------------- } } } Thomas E. Phillips, PhD } Professor of Biological Sciences } Director, Molecular Cytology Core } 3 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } } 573-882-4712 (office) } 573-882-0123 (fax) } PhillipsT-at-missouri.edu } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 11:58:55 2004
Dear Shannon, I have two S-570s and the problem you see is almost certainly a magnetic field affecting the image. Does it translate to 60-cycle from the mains? Does the problem go away at higher magnifications, when the relays in the back "click"? I have seen this when the microscope was a bit too close to a circuit-breaker box or when there was a big instrument that drew a lot of current was "downstream" on the same electrical feed as the SEM or the SEM was too close to the power lines in the room. On the microscope, there are two relays that control magnification at the back-right corner. You can hear them click when you go up and down in mag. You can get two more and replace them, if that is the problem. The other thing that could cause the ripple is a ripple in one of the power supplies. An electronics technician could put an oscilloscope on the various test points and see if that is the problem. Waves and ripples on the image are usually mechanical vibration or mag field, but yours seems too severe to be vibration. Good luck, Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchange.ubc.ca ----- Original Message ----- } From: "Shannan Little" {littlesm-at-agr.gc.ca} To: {Microscopy-at-sparc5.microscopy.com} Cc: "Byron Lee" {lee-at-agr.gc.ca} Sent: Wednesday, February 25, 2004 8:34 AM
We are having problems with our Hitachi S-570 and a service technician is not available until the end of April. Perhaps someone has experienced a similar problem and can give us some advice.
At low magnifications, everything appears to be okay (perhaps because the irregularity is too small to see) but around 2500 X the entire image on the CRT begins to wave. This waviness increases in size when the magnification is increased. For instance, if a straight line is focused on, a regular frequency wave appears to travel along the line. Magnify this image and the wave also magnifies. The problem is accentuated when moving from aperture 3 to aperture 2.
We don't believe any other equipment is causing an external disturbance. We have changed the filament, cleaned the Wehnelt, anode, and movable and fixed apertures. Any ideas on a possible solution?
Shannan
Shannan Little Research Technician/Technicien de recherche Electron Microscopy and Image Analysis / Microscopie électronique et Analyse d'images Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 403-317-3446 Facsimile/Télécopieur: 403-382-3156 P.O. Box 3000 / CP 3000 Lethbridge, Alberta T1J 4B1 littlesm-at-em.agr.ca http://res2.agr.ca/lethbridge/emia/index_e.htm
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 12:27:22 2004
------------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tgardiner-at-mmtinc.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 25, 2004 at 08:21:13 ---------------------------------------------------------------------------
Shannan, I believe you have a classic case of the "jiggies". This phenomenon presents itself when you image a sample that is held loose in the SEM. The part must be clamped firmly to the base to get rid of the jiggies. This issue was addressed in a recent issue of "Microscopy Today".
Stu Smalinskas, P.E. Metallurgist SKF NATC Plymouth, Michigan
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Shannan wrote:
We are having problems with our Hitachi S-570 and a service technician is not available until the end of April. Perhaps someone has experienced a similar problem and can give us some advice.
At low magnifications, everything appears to be okay (perhaps because the irregularity is too small to see) but around 2500 X the entire image on the CRT begins to wave. This waviness increases in size when the magnification is increased. For instance, if a straight line is focused on, a regular frequency wave appears to travel along the line. Magnify this image and the wave also magnifies. The problem is accentuated when moving from aperture 3 to aperture 2.
We don't believe any other equipment is causing an external disturbance. We have changed the filament, cleaned the Wehnelt, anode, and movable and fixed apertures. Any ideas on a possible solution?
Shannan
Shannan Little Research Technician/Technicien de recherche Electron Microscopy and Image Analysis / Microscopie électronique et Analyse d'images Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 403-317-3446 Facsimile/Télécopieur: 403-382-3156 P.O. Box 3000 / CP 3000 Lethbridge, Alberta T1J 4B1 littlesm-at-em.agr.ca http://res2.agr.ca/lethbridge/emia/index_e.htm
__________________________________ Do you Yahoo!? Yahoo! Mail SpamGuard - Read only the mail you want. http://antispam.yahoo.com/tools
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 14:00:37 2004
so you think you want to try cryo-em but you are not sure which procedure will work best for you or you have tried high pressure freezing or the Tokuyasu method but the results didn't come up to expectations.
this is the course for you. Expert help with lots of experience with a multitude of specimens
When: June 8 -June 17, 2004
Where: University of British Columbia, Vancouver, Canada
Main Topics: High Pressure Freezing, Freeze Substitution, Cryo Ultramicrotomy, Tokuyasu Method, Plunge Freezing, Cryo TEM, Cryo SEM, Immunolabelling, Nonogold gold enhancement and fluronanogold gold enhancement
Core Faculty include Kent McDonald (UC Berkekley), Stan Erlandsen (U Minnesota), George Postuma (U Utrecht, Netherlands), Doug Keene (Shriner's Hospital, Portland) Lacey Samuels (UBC), Gethin Owen (UBC), Elaine Humphrey (UBC).
For an application form see the website http://emlab.ubc.ca and click on Third International CryoEM Course
This year we will be using nanogold labelling with gold enhancement (not silver).
This course allows you to follow (with hands-on) the procedure all the way through from freezing to presentation of results.
-- Dr. Elaine Humphrey Director, BioImaging Facility President, Microscopy Society of Canada University of British Columbia 6270 University Blvd, mail-stop Botany Vancouver, BC CANADA, V6T 1Z4 Phone: 604-822-3354 FAX: 604-822-6089 e-mail: ech-at-interchange.ubc.ca website: www.emlab.ubc.ca
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 14:10:54 2004
I suppose a response is not absolutely required, but you catch me in one of those moods.
I had a discussion with a chemist friend years ago about chloroform and its hazards. He strongly made the point that chloroform itself was not so bad as a lot of the paperwork made it out to be. The hitch was that chloroform is often not pure but contaminated with low levels of carbon tetrachloride, a powerful carcinogen. It seemed that chloroform took the rap and has been unable to acquit its name.
Now that is not to say that I am in favor of inhaling chloroform or anything else unnecessarily. (It is definitely affecting Long). We should be very careful to avoid becoming complacent and careless around the chemicals we work with. I just don't like to see the dangers of the chemicals exaggerated. It is a "boy crying wolf" scenario to me. I can get used to discounting the dangers for the chemicals I am familiar with and start discounting the dangers for those chemicals I am not so familiar with.
I suppose I was "poisoned" to such warnings when I read the MSDS for a one-pound bottle of pure calcium carbonate. It allowed for disposal of the material in "an approved chemical waste landfill". I suppose I should have bought mine at the health food store or just scraped some off of the gravel drive out back. That way I could just through the excess away when I was done.
Warren
At 10:47 AM 2/25/2004, you wrote:
} Long } } stop using chloroform immediately. It's harmful and should have been risk } assessed - not only is it an anaesthetic it is harmful over a prolonged } exposure by inhalation and a category 3 carcinogen. We only use chloroform } in the fume hood these days. } } The safest method is to buy a heat pen - available at most e.m suppliers. } This uses a hot tungsten wire to soften and spread your sections. } } Malcolm } } Malcolm Haswell } e.m. unit } School of Health, Natural and Social Sciences } Fleming Building } University of Sunderland } Tyne & Wear } UK } } ----- Original Message ----- } } From: Long Miao {lmiao-at-bio.fsu.edu} } Date: Wednesday, February 25, 2004 3:37 pm } Subject: [Microscopy] semi-thick sections wrinkles spreading? } } } } Dear list, } } } } During the past month, I have been working on semithick } } sectioning. The } } sections are around green-purple , which translates into thickness of } } 120-250 micron. To spread the wrinkles , I used Chloroform and it } } workedfine. However, it made me very sick, shortness of breath. } } I am wondering } } if any of you have better and safer methods to spread the thick } } sections. } } Thank you, } } } } Long } } } } } } ----------------------------------------------- } } Miao, Long } } Dept of Biological Science } } 334 Bio. Unit1 } } Florida State University } } Tallahassee, FL32306-4370 } } email: lmiao-at-bio.fsu.edu } } Voice: (850)644-9817 } } FAX : (850)644-0481 } } -----------------------------------------------
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 14:32:18 2004
} Shannan, I believe you have a classic case of the } "jiggies". This phenomenon presents itself when you } image a sample that is held loose in the SEM. The } part must be clamped firmly to the base to get rid of } the jiggies. This issue was addressed in a recent } issue of "Microscopy Today".
There is a possibility the problem could be electromagnetic interference. If the "jiggies" are mimimized for higher acceleration voltages, then it's likely this type of interference. Otherwise, look for something like an un-dampened vacuum pump hose.
} Shannan wrote: } } We are having problems with our Hitachi S-570 and a } service technician is not available until the end of } April. Perhaps someone has experienced a similar } problem and can give us some advice. } } At low magnifications, everything appears to be okay } (perhaps because the irregularity is too small to see) } but around 2500 X the entire image on the CRT begins } to wave. This waviness increases in size when the } magnification is increased. For instance, if a } straight line is focused on, a regular frequency wave } appears to travel along the line. Magnify this image } and the wave also magnifies. The problem is } accentuated when moving from aperture 3 to aperture 2. } } } We don't believe any other equipment is causing an } external disturbance. We have changed the filament, } cleaned the Wehnelt, anode, and movable and fixed } apertures. Any ideas on a possible solution? } } Shannan } } Shannan Little } Research Technician/Technicien de recherche } Electron Microscopy and Image Analysis / } Microscopie ilectronique et Analyse d'images } Agriculture and Agri-Food Canada/Agriculture et } Agroalimentaire Canada } Telephone/Tiliphone: 403-317-3446 } Facsimile/Tilicopieur: 403-382-3156 } P.O. Box 3000 / CP 3000 } Lethbridge, Alberta T1J 4B1 } littlesm-at-em.agr.ca } http://res2.agr.ca/lethbridge/emia/index_e.htm } } __________________________________ } Do you Yahoo!? } Yahoo! Mail SpamGuard - Read only the mail you want. } http://antispam.yahoo.com/tools } } }
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 14:58:30 2004
Try Epon 812 (or whatever substitute you prefer) 40g, DDSA 10g, NMA 20g. If you have a rotator (the over-and-over type, not the circular motion type) available, that helps, and you should extend your infiltration times considerably. Add at least one extra change into freshly-made Epon mix plus DMP30 (2%) and if possible leave it in the last change in the fridge under vacuum overnight, before embedding in yet more Epon mix plus DMP30. After embedding, leave it in a vacuum oven at 37 C for 48 hours then an ordinary oven at 60 C for 7 days. This is my protocol for embedding blocks that are not only bone but also very large - you might not need to be quite so extreme for your material.
Lesley Weston.
on 24/02/2004 1:25 PM, Dusevich, Vladimir at DusevichV-at-umkc.edu wrote:
} } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- --} - } } Hi, } } What is a good embedding media for bone ultramicrotomy? } I use Epon 812 and Spurr resin, but penetration is not really } good enough. } } Thanks, } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 3127 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } }
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 15:01:08 2004
It's always difficult to make any educated guesses about things like this without seeing the images. However the one thing that puzzles me is that it would be affected by a change in aperture size. If that's true I can only think of two possibilities. First, you may have some charging in your column or your sample and when you change the aperture you are changing the beam current and therefore changing the charge/discharge rate of whatever is charging. I'm not familiar with the Hitachi s-570 but if the aperture is heated, the second possibility is ripple from the heater supply. I know the old JEOL-35 had a heated aperture and ripple from the heater supply was a common problem.
Good Luck, Bill
Shannan Little wrote:
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From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 15:05:40 2004
Heat pens are good (Ted Pella sells them), but you might be able to remove wrinkles by using a small wire loop to transfer the thick sections to a drop of filtered de-ionised water on a slide, then leaving the slide on a hot-plate set at about 60 C until the water is gone. Either method is much safer than chloroform.
Lesley Weston.
on 25/02/2004 8:19 AM, Paula Sicurello at patpxs-at-gwumc.edu wrote:
} } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- --} - } } Have you heard of a heat pen? Theycan be used to get rid of wrinkles. You } hold it over the sections like you do the chloroform stick and the wrinkles } should go away. I don't know which EM vendor has them, but it used to be tha } all of the EM vendors sold them. } } Good luck and please get some fresh air, } } Paula :-) } } Paula Sicurello } George Washington Univ. Medical Center } Electron Microscope Lab } Washington, DC 20037 } 202-994-2930 phone } 202-994-2518 fax } } } } "Long Miao" {lmiao-at-bio.fsu.edu} 02/25/04 10:51 AM } } } } } } ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- --} - } } Dear list, } } During the past month, I have been working on semithick sectioning. The } sections are around green-purple , which translates into thickness of } 120-250 micron. To spread the wrinkles , I used Chloroform and it worked } fine. However, it made me very sick, shortness of breath. I am wondering } if any of you have better and safer methods to spread the thick sections. } } Thank you, } } Long } } } ----------------------------------------------- } Miao, Long } Dept of Biological Science } 334 Bio. Unit1 } Florida State University } Tallahassee, FL32306-4370 } email: lmiao-at-bio.fsu.edu } Voice: (850)644-9817 } FAX : (850)644-0481 } ----------------------------------------------- } } } } }
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 15:38:28 2004
I had asked about microprobes late last year and got several leads on probes. I was following the Kleindiek MM3A probes but found that they will only work up to 100C. I need in-SEM probes that will work up to about 175C. They need to be able to handle about 10mA DC and be positionable to roughly 0.5u.
Any other ideas?
thanks, gary g.
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 16:18:51 2004
We had some interaction with people at Zyvex (www.zyvex.com) regarding their MEMS based tools. You might want to contact them with your questions.
I also have this contact info for Peter Overland Zyvex Corporation Tel. 972-235-7881 ext 249 or ask for Phil Foster (Southwest rep)
Disclaimer: I have no financial interest in the company.
Jerzy
****************************************************** Jerzy Gazda, Ph.D. Advanced Micro Devices Supervising Engineer 5204 E. Ben White Blvd. - MS 512 PCAL - AIM Section Austin, TX 78741 TEL: 1-800-538-8450, Ext. 51453 jerzy.gazda-at-amd.com ******************************************************
-----Original Message----- } From: Gary Gaugler [mailto:gary-at-gaugler.com] Sent: Wednesday, February 25, 2004 4:11 PM To: MSA listserver
Greeting listers:
I had asked about microprobes late last year and got several leads on probes. I was following the Kleindiek MM3A probes but found that they will only work up to 100C. I need in-SEM probes that will work up to about 175C. They need to be able to handle about 10mA DC and be positionable to roughly 0.5u.
Any other ideas?
thanks, gary g.
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 16:18:11 2004
We have a S-570 ourselves. Some questions: What kVs are you using? Does the problem get worse at a lower kV? The 570's column could be better shielded, and they're sensitive to EMF variations at 5kV and less. Just moving in a chair when one is within 3 feet of the column will move the image. So an EMF field from lights, computers and computer monitors can cause this. You can get a Gauss meter fairly cheaply, and it's worthwhile having one to check for fields. I moved the computer to 6' from the column, and that helped with a problem we had. Does the problem lessen or go away with slower scan speeds? Another indication of EMF problems.
What working distance? Does the problem get worse at a longer WD? If yes, this indicates mechanical vibrations. One thing to check on the 570 is the "shock absorbers" on which the column rests. In each corner, just below the console. If the rubber accordion sleeves are cracked, they're no good. You should be able to get new ones from Hitachi -- supposedly much cheaper from Hitachi Canada than Hitachi US. Our service engineers tell me to fill a bad mount with silicone chaulk. Layer in a bunch, let it set, layer in more, let it set, repeat until the mount is full. This does seem to work, and it's cheaper than buyng mounts. But, if you have a service contract, this should pay for new mounts (maybe).
Did things change at all when you cleaned the apertures, etc.? If yes, they may just need recleaning, especially if you used EtOH or acetone as your final cleaning steps. Even absolute EtOH an 100% acetone leave residues. The final rinse should be in the best water you can get, then oven dry. The column may also need cleaning, not just the apertures.
Do you have a Faraday cup? If yes, does the current vary at the same frequency as the image waves? Does the emission current vary? If yes to either, you may have gun problems.
Do you get bright flashes when using the stage rotation control? If yes, then the stage is likely not grounded properly. The wire that grounds the stage specimen mount could be better placed, and can get worn or broken. Our wire wore through under one on the mounts (the little "U" bracket), so that it shorted to the stage, which it's not supposed to do. Bad grounding can cause the waviness you're seeing. Take an ohmmeter to the capped plug (the cap is on, right?) on the left side of the stage door. There should be no resistance from the center pin to the specimen holder mount, but there should be an "open" between the holder mount and the stage itself (try the flat plate immediately to the right of the holder mount).
Phil
} We are having problems with our Hitachi S-570 and a service technician } is not available until the end of April. Perhaps someone has experienced } a similar problem and can give us some advice. } } At low magnifications, everything appears to be okay (perhaps because } the irregularity is too small to see) but around 2500 X the entire image } on the CRT begins to wave. This waviness increases in size when the } magnification is increased. For instance, if a straight line is focused } on, a regular frequency wave appears to travel along the line. Magnify } this image and the wave also magnifies. The problem is accentuated when } moving from aperture 3 to aperture 2. } } We don't believe any other equipment is causing an external } disturbance. We have changed the filament, cleaned the Wehnelt, anode, } and movable and fixed apertures. Any ideas on a possible solution? } } Shannan } } Shannan Little } Research Technician/Technicien de recherche } Electron Microscopy and Image Analysis / } Microscopie électronique et Analyse d'images } Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada } Telephone/Téléphone: 403-317-3446 } Facsimile/Télécopieur: 403-382-3156 } P.O. Box 3000 / CP 3000 } Lethbridge, Alberta T1J 4B1 } littlesm-at-em.agr.ca } http://res2.agr.ca/lethbridge/emia/index_e.htm
-- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax)
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 17:43:22 2004
By coincidence, someone just walked in with a copy of Microscopy Today September/October 2003 which contains a Microscopy 101 article on zigzag edges in SEM micrographs. I've had jaggies from 60Hz interference, from vibration and, less intuitively, when a component of the stage was loose.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 17:46:11 2004
Geoff, The PELCO Heat Pen has a nichrome element that becomes incandescent during use. A red hot tip like our Heat Pen has gives off a large amount of radiant heat. This results in more efficient heating of the sample at a safer distance. A hot tip that is not glowing red would have to be brought close enough to the sample to heat it by air convection/conduction which would take longer and put the sample at risk. Some other advantages are: Variable element power (heat), quick response time of element temperature when setting is changed, the level of the heat is visually indicated by a 10 color LED bar and a lightweight easily manipulated heating element wand.
Disclaimer: I work for Ted Pella, Inc which is the company that manufactures the PELCO Heat Pen.
Regards, Mark Armogida VP Production and Engineering Ted Pella, Inc.
Phone: 530-243-2200 ext. 212 Fax: 530-243-3761
mark_armogida-at-tedpella.com www.tedpella.com
-----Original Message----- } From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu] Sent: Wednesday, February 25, 2004 12:53 PM To: Tom Phillips Cc: Long Miao; Microscopy-at-msa.microscopy.com
Instead of a $300 heat pen how about a soldering iron/gun?
Geoff
Tom Phillips wrote:
} -------------------------------------------------------------------------- ---- } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- ----- } } } I bought a heat pen from Ted Pella (I have no financial interests in } this company). It was expensive ($300??) but a lot better than the } small battery operated units (~$40). I liked it so much, I bought two } more for my other ultramicrotomes. We use it on both thin and } semi-thin. It is a lot better and safer than chloroform (long term } liver problems can result from repeated inhalation). good luck } } } 10:37 AM 2/25/2004 -0500, you wrote: } } } } ------------------------------------------------------------------------- ----- } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.msa.microscopy.com/MicroscopyListserver } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------- ------ } } } } } } Dear list, } } } } During the past month, I have been working on semithick sectioning. The } } sections are around green-purple , which translates into thickness of } } 120-250 micron. To spread the wrinkles , I used Chloroform and it } } worked } } fine. However, it made me very sick, shortness of breath. I am } } wondering } } if any of you have better and safer methods to spread the thick } } sections. } } } } Thank you, } } } } Long } } } } } } ----------------------------------------------- } } Miao, Long } } Dept of Biological Science } } 334 Bio. Unit1 } } Florida State University } } Tallahassee, FL32306-4370 } } email: lmiao-at-bio.fsu.edu } } Voice: (850)644-9817 } } FAX : (850)644-0481 } } ----------------------------------------------- } } } Thomas E. Phillips, PhD } Professor of Biological Sciences } Director, Molecular Cytology Core } 3 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } } 573-882-4712 (office) } 573-882-0123 (fax) } PhillipsT-at-missouri.edu } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 17:45:58 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jennibean73-at-yahoo.com) from http://www.msa.microscopy.org/Ask-A-Microscopist.html on Wednesday, February 25, 2004 at 12:39:09 ---------------------------------------------------------------------------
Email: jennibean73-at-yahoo.com Name: Jen
Organization: N/A
Education: Graduate College
Location: City, State, Country
Question: I am counting bacteria at 1000X magnification. How would one calculate an approximation of bacterial counts in the entire sample?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ldemp-at-mse.ufl.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 25, 2004 at 13:25:21 ---------------------------------------------------------------------------
Florida Society for Microscopy (FSM), Florida Chapter of the AVS Science and Technology Society (Fl AVS), Florida Section of the American Ceramic Society (Fl ACerS) 2004 Annual Joint Symposium March 8-9, 2004, University of Central Florida Student Union, Orlando, Florida Web: www.flavs.org This is a reminder that the Program for the FL AVS, FSM and Fl-ACerS 2004 Annual Joint Symposium, being held March 8-9, 2004, at UCF, in Orlando, Fl, is available online. Technical Sessions include: Thin Films, Electronic Materials, Microscopy in the Physical and Biological Sciences, FIB Investigations of Mechanical Damage, Ceramic Coatings, MicroElectroMechanicalSystems (MEMS), Biomaterial Interfaces, Nanoscience, and Nanotechnology. Please visit our website at www.flavs.org for the program schedule and additional information on the symposium. There is no registration fee to attend the symposium, reception, or equipment exhibit. On-line registration is suggested at http://www.flavs.org/RegForm.htm The Equipment Exhibit will be held on Monday and Tuesday, March 8-9, 2004, in the UCF Student Union Pegasus Ballroom, in conjunction with the poster session, reception and coffee breaks. The exhibit will be opened from 11 am to 7 pm on Monday, and from 10 am to 3 pm on Tuesday. On-line vendor registration is available at http://www.flavs.org/vendorregform.htm. For more information on the exhibit or to be an exhibitor, please refer to http://www.flavs.org/Equipment.htm. Two AVS Short Course
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (info-at-klughammer.de) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, February 23, 2004 at 23:41:32 ---------------------------------------------------------------------------
I am looking for a glass slide which is covered by a flouerscent material. I need it for calibration purposes. Does anybody know where to get it or how to make it. I already used a text marker. This works but it was too week and very irregular.
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From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 18:42:29 2004
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (donaldawbrey-at-texashealth.org) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 25, 2004 at 18:34:32 ---------------------------------------------------------------------------
Email: donaldawbrey-at-texashealth.org Name: Donald G. Awbrey
We have a set of fluorescence calibration slides available that might suit your needs. Please visit www.MicroscopyEducation.com and look for FluorRef(TM).
Good hunting, Barbara Foster Microscopy/Microscopy Education, Inc. (MME, Inc.) 125 Paridon Street, Suite 102 Springfield, MA 01118 PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
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At 06:42 PM 2/25/04 -0600, Klughammer - Anneliese Schmaus (by way of wrote:
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From MicroscopyL-request-at-ns.microscopy.com Wed Feb 25 23:37:52 2004
www.anaspec.co.za Hi all I have seen a few queries around the possibility of fields affecting a system. Can I point you to the RS Electronics website where you can buy a magnetic field tester which will allow you to find hese fields very quickly and easily.
If you ask your local RS Electronics store about it I am sure they can help.
The RS Electronics description and part number are:
ELF Magnetic 212-837 Price is £140.00
http://rswww.com/
and for the USA http://www.rselectronics.com Good luck
Tel: +27 11 794 8340 Fax: +27 11 794 8349 P.O. Box 2561 Honeydew 2040 Gauteng, South Africa
-----Original Message----- } From: Mary Mager [mailto:mager-at-interchange.ubc.ca] Sent: 25 February 2004 08:10 To: Shannan Little Cc: Microscopy
Dear Shannon, I have two S-570s and the problem you see is almost certainly a magnetic field affecting the image. Does it translate to 60-cycle from the mains? Does the problem go away at higher magnifications, when the relays in the back "click"? I have seen this when the microscope was a bit too close to a circuit-breaker box or when there was a big instrument that drew a lot of current was "downstream" on the same electrical feed as the SEM or the SEM was too close to the power lines in the room. On the microscope, there are two relays that control magnification at the back-right corner. You can hear them click when you go up and down in mag. You can get two more and replace them, if that is the problem. The other thing that could cause the ripple is a ripple in one of the power supplies. An electronics technician could put an oscilloscope on the various test points and see if that is the problem. Waves and ripples on the image are usually mechanical vibration or mag field, but yours seems too severe to be vibration. Good luck, Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchange.ubc.ca ----- Original Message ----- } From: "Shannan Little" {littlesm-at-agr.gc.ca} To: {Microscopy-at-sparc5.microscopy.com} Cc: "Byron Lee" {lee-at-agr.gc.ca} Sent: Wednesday, February 25, 2004 8:34 AM
We are having problems with our Hitachi S-570 and a service technician is not available until the end of April. Perhaps someone has experienced a similar problem and can give us some advice.
At low magnifications, everything appears to be okay (perhaps because the irregularity is too small to see) but around 2500 X the entire image on the CRT begins to wave. This waviness increases in size when the magnification is increased. For instance, if a straight line is focused on, a regular frequency wave appears to travel along the line. Magnify this image and the wave also magnifies. The problem is accentuated when moving from aperture 3 to aperture 2.
We don't believe any other equipment is causing an external disturbance. We have changed the filament, cleaned the Wehnelt, anode, and movable and fixed apertures. Any ideas on a possible solution?
Shannan
Shannan Little Research Technician/Technicien de recherche Electron Microscopy and Image Analysis / Microscopie électronique et Analyse d'images Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 403-317-3446 Facsimile/Télécopieur: 403-382-3156 P.O. Box 3000 / CP 3000 Lethbridge, Alberta T1J 4B1 littlesm-at-em.agr.ca http://res2.agr.ca/lethbridge/emia/index_e.htm