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Dear Joel,

Thanks for your comments. The ultra-microtome as such was of course invented by Fritiof Sjostrand and I agree that his first models had a remote motor, just as you describe. I did not realize that his design also originally had 360� rotation of the specimen chuck holder. As far as I know his design was developed in conjunction with LKB-Produkter, Bromma Sweden whereas the slightly later Fernandez-Moran instrument was developed with Ernst Leitz, Wetzlar Germany. I seem to recollect that an example of the F-M instrument found its way to Dunedin, New Zealand, but there must have been others too.
Perhaps others might be interested in taking the story further from here....
Cheers,

Jim

-----Original Message-----
} From: Joel Sheffield [mailto:jbs-at-temple.edu]
Sent: Thursday, August 05, 2004 3:05 PM
To: James Chalcroft; Microscopy ListServer

Dear Jim,

I saw the Sjostrand microtome when I taught a course at Rutgers,
Camden, in the 1970's. We actually got it set up and running. They
also had a (barely) working RCA EMU-2 scope. I doubt if they have
kept either.

The other thread in this history, of course, was the system developed
by Keith Porter. In the original models of what eventually became
the Porter-Blum microtome, the block followed the famous
parallelogram path, but advance was thermal. We had a goosneck lab
light mounted over the rod that held the specimen, and you sat there
flashing the light with each pass of the block. If you were good,
you could get beautiful ribbons of silver --but woe betide anyone who
walked past you while you were sectioning!

Later on, Keith developed the offset gimble mechanical advance that
led to the Sorval series of MT microtomes.

The other interesting approach to microtomy was that of Huxley, who
used flexible springs for all of the hinge points, to replace
bearings and reduce vibration. This machine was originally made by
Cambridge, and later distributed by LKB.

} Dear Joel,
}
} Thanks for your comments. The ultra-microtome as such was of course
} invented by Fritiof Sjostrand and I agree that his first models had a
} remote motor, just as you describe. I did not realize that his design
} also originally had 360� rotation of the specimen chuck holder. As far
} as I know his design was developed in conjunction with LKB-Produkter,
} Bromma Sweden whereas the slightly later Fernandez-Moran instrument
} was developed with Ernst Leitz, Wetzlar Germany. I seem to recollect
} that an example of the F-M instrument found its way to Dunedin, New
} Zealand, but there must have been others too. Perhaps others might be
} interested in taking the story further from here.... Cheers,
}
} Jim
}
} -----Original Message-----
} From: Joel Sheffield [mailto:jbs-at-temple.edu]
} Sent: Thursday, August 05, 2004 3:05 PM
} To: James Chalcroft; Microscopy ListServer
} Subject: [Microscopy] Re: RE: Leica ultra-microtome (History)
}
} I do remember setting up something like that. Except my recollection
} is that it was designed by Sjostrand. The cylindrical chuck unit had
} a groove around it so that you would mount the motor on the wall, and
} run a long V-belt from the motor to the microtome. This was to
} minimize vibration. The other feature that I remember is that the
} cutting speed was quite fast, not at all like what we use today.
}
} Joel
}
}
} Subject: [Microscopy] RE: Leica ultra-microtome (History)
} Date sent: Thu, 5 Aug 2004 10:08:02 +0200 From:
} "James Chalcroft" {jchalcro-at-neuro.mpg.de} To: "David
} Elliott" {David.Elliott-at-yale.edu} Copies to: "Microscopy
} ListServer" {Microscopy-at-MSA.Microscopy.Com}
}
} }
} }
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} }
} } The Leica ultra-microtome ....?!
} } This brings back memories of the original one, based on the design
} } by Humberto Fernandez-Moran about 50 years ago. Anyone still
} } remember it? Its design was absolutely unique in that the specimen
} } chuck was mounted in a motor-driven massive cylinder which performed
} } complete rotations in the horizontal plane over two pairs of huge
} } flat sapphire bearings placed in a "V" orientation. I imagine that
} } it must have been a "cow of a thing" to set up! It was possibly
} } developed to utilize the newly invented diamond knives (also
} } invented by F-M, whose genius contributed so much to new
} } developments in the early days of TEM). I only ever saw advertising
} } brochures of the ultra-microtome, so would be interested to learn of
} } users' experiences from those times and perhaps also something about
} } the fate of these now-historic instruments.
} }
} } -----Original Message-----
} } } From: David Elliott [mailto:David.Elliott-at-yale.edu]
} } Sent: Wednesday, August 04, 2004 5:09 PM
} } To: Microscopy ListServer
} } Subject: [Microscopy] Leica ultra-microtome
} }
} }
} }
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} }
} } Hi all
} } I am looking at Leica ultra-microtomes. The new version (the UC6b)
} } has two controllers, the key pad or the touch screen. I would
} } appreciate any input or comments on these. I am especially
} } interested in reasons to get one or the other. Thanx David
} }
} }
} } _____________________
} }
} } David Elliott Ph.D.
} } Research Assistant Professor
} } Department of Cell Biology and Anatomy
} } PO Box 245004
} } Tucson, AZ 85724
} }
} } Voice: 520-626-7870
} } Fax: 520-626-2097
} }
} }
} }
} }
}
} Joel B. Sheffield, Ph.D
} Department of Biology
} Temple University
} Philadelphia, PA 19122
} Voice: 215 204 8839
} e-mail: jbs-at-temple.edu
}
}

Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs

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Leica ultratomes originated from Ultracut series by Reichert-Jung. Am I
correct? It seems to me, Leica did not have their own ultratome
development (would be nice to know details if they did). They just bought
Reichert-Jung and LKB both. They killed SuperNova (LKB) and continued
Ultracut. Sergey

At 01:08 AM 8/5/2004, you wrote:

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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

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Aha! Joel, Sergey and anyone else interested in historical matters,

I got motivated after reading your interesting comments and actually located here an old Leica brochure (53-12b, in German) dated June 1964, which described the original "Leica Ultra-Mikrotom nach Fern�ndez-Mor�n". It was equipped with a Rawyler diamond knife "of highest quality" and was apparently designed to cut just about anything. The accompanying TEM images show sectioned cells in Vestopal and Methacrylate also an Al/Ag alloy. The 3 kg steel rotor was belt-driven with 2 motors situated below the desk (one was used for the sectioning phase of rotation and cutting speed could be controlled from 50 down to 3 mm/sec, while the fast second motor took over for the rest of the rotation cycle).
The object chuck was at the front of a long diametrically placed cylindrical invar holder (fastened at its back end to the back of the rotor) which had an inbuilt heating element, and the free front end moved by thermal expansion towards the knife for up to 30 mins. After that period the complete holder was removed and allowed to cool down for 15 mins, during which time a second holder could be used for sectioning.
Visualization during sectioning was done with a 72x (Leitz Greenough-type?) stereomicroscope and associated fluorescent lamp. A plexiglas hood was supplied to protect the system from air movements and temperature fluctuations during sectioning.
Other statistics: Weight (+ table) was 148 kg. Power draw-off was 130 W.
The images shown corresponded to typical good quality methacylate sections of the 1950/60s but the instrument never became popular. I imagine that this brochure was one of the last that Leitz issued.
It would seem that the main advantage of this instrument design was that complete 360� rotation obviated the need for specimen retraction. Retraction during the upwards stroke is absolutely necessary for any reciprocating system in order to prevent wetting of the knife front or even breakage of the delicate cutting edge when the specimen moves upwards after cutting because all thermal expansion systems have great inherent inertia and advance cannot be stopped successfully for such short time periods.
One possible disadvantage of the system is the need for rotational bearings.
I do remember vaguely another old ultra-microtome brochure from Leica which had a graph showing the excellent reproducibility of specimen position during cutting from one cycle to the next. This may be so (since German precision workmanship in those years was unbeatable) but the inherent fine-scale bearing rumbling, even if reproducible, would result in a slightly corrugated cut as the specimen would oscillate a little forwards and backwards with respect to the knife during cutting. Perhaps it would need only 10 nm lateral movements in a 100 mm thick section to be noticeable when highest quality ultra-thin sections were examined. Perhaps I am talking somewhat off the top of my head in this case but it would now be interesting to hear from others the real reason(s) for the unpopularity of this long-discontinued instrument - the very FIRST Leica Ultra-Microtome.
Best regards & good sectioning to you all ...

Jim

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Hello folks,

I have a Gatan mod. 681 Ion Beam Coater and I'm looking for targets for
it. I'm on a limited budget so I'd rather not pay Gatan's prices.
Anyone have sources or ideas for Au and Ti targets?

Jerry

--
Gerald Bourne
Major Analytical Instrumentation Center
Department of Materials Science and Engineering
University of Florida
100 Rhines Hall
P.O. Box 116400
Gainesville, FL 32611
(352) 392-6985
(352) 392-0390 fax

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Dear all,

We have a QImaging camera Retiga 1300i 12 bits. The company we deal with for
the image acquisition (with a motorized microscope) uses the 16 bit format.
So, they transform the 12 bit images to 16 bit images.

We want to use deconvolution on these images. Will the transformation to 16
bits impair image quality?

Thank you, have a good week-end!

Marie-Claude Belanger

_________________________________________________________________
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maintenant � profiter de tous les avantages de MSN Premium et obtenez les
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Jerry:

While we do make our own Ion Beam Sputter Deposition and Etching System,
we can supply targets for the Gatan system as well. You will find our
prices to be *very* reasonable. Please contact me off-line about your
specific requirements and I will send you a quote.

Best regards-

David

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.

Gerald Bourne wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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}
}
} Hello folks,
}
} I have a Gatan mod. 681 Ion Beam Coater and I'm looking for targets
} for it. I'm on a limited budget so I'd rather not pay Gatan's
} prices. Anyone have sources or ideas for Au and Ti targets?
} Jerry
}

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Image quality is highest using the 16-bit format.
Since the original image is 12-bits, image
quality would suffer (mostly due to loss of dynamic
range) if converted to 8-bits. The conversion
from 12-bits to 16-bits is typically accomplished
by zero filling the four high order bits. Consequently,
the image data is in all eight bits of the low order
byte and in the four low order bits of the high order byte.

If your deconvolution program can handle 16-bit images,
there should be no image degradation. Be advised that,
depending on the app itself, your memory requirements
may be more than you have available. Very often one
can get "Out of Memory" error messages. With modest
pixel density and 16-bit TIFF files, you should be OK.

gary g.

At 10:04 AM 8/6/2004, you wrote:

} Dear all,
}
} We have a QImaging camera Retiga 1300i 12 bits. The company we deal with
} for the image acquisition (with a motorized microscope) uses the 16 bit
} format. So, they transform the 12 bit images to 16 bit images.
}
} We want to use deconvolution on these images. Will the transformation to
} 16 bits impair image quality?
}
} Thank you, have a good week-end!
}
} Marie-Claude Belanger

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Hendrik

We also have our 2010F running on a UPS system. I have just done some
measurements and while, yes, there is a very large field at the unit
(} 500mG rms - off scale for my EMF meter) this decays away rapidly and
drops below 1mG rms seven feet from the unit. In our case the column is
about 15' from the UPS and we see no effect.

We are still using the same batteries as delivered in 1998. The 50% life
time in the case of a power failure has diminished but they still provide
sufficient protection for the field emission tip. The battery
specifications from the manufacturer states 200 complete full load
discharges - we were told a typical life was 4-5 years depending on the
number of excursions. Through the manufacturer replacement of all the
batteries was quoted at $5K.

One thing to beware of, on multi-phase UPS systems if you are prone to one
phase ONLY dying you can fry the UPS control board and you will need a
voltage regulator before the UPS unit to protect the UPS.

Regards

Alan

At 11:08 AM 7/23/2004 -0700, Mardinly, John wrote:

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Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu

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Robert:

We have a ArtixScan 2500. (as well as two Agfa Duoscan's). The
software interface was a little cumbersome to use, but the image quality was
very good.

However, I strongly recommend against Microtek. On the 2500 the lamp
stays on all the time (i.e. no standby) - o.k., not a problem just wear on the
lamp. The lamp burned out after 8 months - o.k., no problem we replaced
many lamps in previsouly years on several scanners - 5 minutes later lamp in
hand. . . Can NOT get a replacment lamp. We've been looking every where.
Microtek will NOT sell the lamps, they wanted us to send the scanner (67 lbs)
to across country and they would sell us a new shipping box. Fine - but 7
additional months later they can't come up with a shipping box, nor can they
come up with a price for the lamp replacment and NOW they are telling us
we're out of the 12 month warranty.

So here we sit - $3k scanner useless and for a $30 part.

}
} I am looking for comment on overall functionallity of the Microtek
} ArtixScan 1800f scanner. Reliability, resoultion, speed. Will use this
} to scan EM neg. etc.
}
} Thanks
}
} Robert J. Kayton, Ph.D.
} C.R.O.E.T. L606
} Oregon Health & Science Univ.
} kayton-at-ohsu.edu
} 503-494-2504-Lab
} 503-703-3938-Cell
} www.ohsu.edu/croet/facilities/emicroscopy
}
}

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

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University of Connecticut
Institute of Materials Science

Postdoctoral Position
SPM manipulation of carbon nanotubes and biological systems

A post-doctoral position is available immediately within the Institute
of Materials Science at UConn to work on SPM imaging, manipulation, and
lithography of carbon nanotubes and biological systems. The ideal
candidate will have experience with AFM measurements in liquid, SPM
lithography, and or SPM technique development. Experiments will be
conducted in the newly constructed NanoMeasurement labs, featuring two
new AFM systems for in vitro and air measurements with simultaneous
confocal microscopy, as well as a UHV AFM/STM.

This position is a one-year appointment, with funding available for
further years. To apply, please send a complete resume, together with a
list of publications and contact details for 3 references, to Prof.
Bryan Huey (bhuey-at-ims.uconn.edu). Screening of applications will begin
immediately, and will continue until the position is filled.
Applications are encouraged from under-represented groups, including
minorities, women, and people with disabilities.

Bryan D. Huey
The Institute of Materials Science
University of Connecticut
97 N. Eagleville Road, unit 3136
Storrs, CT 06269 USA
office: (860) 486 3284
fax: (860) 486 4745
http://www.ims.uconn.edu/
bhuey-at-uconn.edu

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (gazzzoman-at-yahoo.com) from
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
Thursday, July 29, 2004 at 09:21:46
---------------------------------------------------------------------------

Email: gazzzoman-at-yahoo.com
Name: daniele gazzola

Organization: Biomed dept. -at-Brown Univ., Providence, RI, USA -
Engineering dept. -at-Genova University, Italy

Title-Subject: [Microscopy] [Filtered] hybrid AFM-optical microscope

Question: I am trying to set up a hybrid microscope in order to use
AFM (atomic force microscope) along with light microscopy(phase) on
unstained biological samples.

The AFM that I am using adds two lenses to the optical path just
above the sample. Unfortunately I neither know the specifications of
those lenses, nor the characteristics of the other optical elements.
I know that the plane of the sample is conjugated to a plane 32mm
above the top lens. The whole AFM is practically a tube about 10cm
long and 1cm of diameter.

I am trying to get informations about the optical elements of a long
working distance condenser. Can anyone please send me a diagram?

I think that the AFM will add one conjugate focal plane to the
system. Do you think that this will be a problem?
What about using reflected light (for brightfield, phase, or DIC)?

I would like any comment or idea about this.
thanks a lot,
daniele

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Frida.Maiers-at-co.hennepin.mn.us) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, July 30, 2004 at 08:32:06
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Email: Frida.Maiers-at-co.hennepin.mn.us
Name: Frida Maiers

Organization: Hennepin County Medical Center

Title-Subject: [Microscopy] [Filtered] MListserver: TEM photographic supplies available

Question: Due to digital camera installation we have the following
available for a nominal fee: 1 case of D19 developer, 11 boxes of EM
film (Kodak 4489, new formulation), 15 liquid kits of Kodak fixer,
and 17 boxes Kodak Polymax II RC photographic paper (100 sheets
each). Thank you.

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (juan-at-nanostellar.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
July 30, 2004 at 22:47:04
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Email: juan-at-nanostellar.com
Name: Juan

Title-Subject: [Microscopy] [Filtered] Export EDX spectrum

Question:
Hi,

I took some EDX spectrum by using EDAX Phoenix 1998 Revision 3.1, but
I can not export the spectrum into a data file. Does anybody know
the method? Any suggestion would be highly appreciated.

Juan

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Gsalaj7-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Sunday, August 1, 2004 at 14:15:07
---------------------------------------------------------------------------

Email: Gsalaj7-at-aol.com
Name: Sally Thomas Graziano

Organization: Orlando Science Center

Education: Undergraduate College

Location: Orlando Florida

Question: In my work on the SEM here at OSC. Along with our
educational programs we have an ongoing research project. In that
regard I have a very small eye cyst from a swan which I need to look
at. As you can see most of my specimens are larger biological
specimens. How should I mount it on the SEM stub. I use carbon tape
for most things but am afraid the cyst will get lost in one of the
bubbles of the tape.Also how long to coat. Any sugestions would be
most helpful. Sally Graziano

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Juan,

You wrote:

} I took some EDX spectrum by using EDAX Phoenix 1998 Revision 3.1, but I
} can not export the spectrum into a data file. Does anybody know the method?
} Any suggestion would be highly appreciated.
}
EDAX have a free spectrum viewer that can be downloaded from:
http://www.edax.com/support/EDS_Spectrum_Viewer.html

You can cut and paste the channel data from this programmme.

Alternatively, I have written an application which will display EDAX .spc
files - as well as Noran, Link/Oxford and EMSA file formats - which allows
export of data as well. Let me know if you want a copy.

David Vowles
Electron Microscope Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 (0)1223 334325
Fax: +44 (0)1223 334567
Email: djv23-at-cam.ac.uk

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Hey Listers,

We are looking for a source of individual metallographic sample storage
containers. Our mounts are typically 1" diameter and 0.5-1" tall.

Anyone have a source for these?

William Giles
Senior Electron Microscopist
Metallography Lab Coordinator
P.O. Box 2128
Henderson, Nevada 89009
(702) 566-4436
(702) 564-9038 (Fax)
William.Giles-at-timet.com

*

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Hi

Can someone point me in the direction of online information regarding
techniques for the identification of asbestos by SEM and by other
techniques?

thanks

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599
ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand

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Let us know if you find something that is suitable and *inexpensive*.
Microscopy supply vendors sell plastic boxes, but at the rate I go through
samples, I would go broke and run out of storage room as well.

No inexpensive vials, jars, etc. that I have found protect the prepared
surface well - no mounting method. The best compromise is to use a snugly
fitting "Cap Plug" (poly closure used to close pipe ends, other holes) and
partially insert the mount into it.

If nothing else fits, I stuff the large mounts into a locking closure
plastic bag. It is likely that if fragile or coated, this will harm the
specimen, however.

A great solution (not my idea) for 1/2" (12mm) pin-mounts is an "Opticlear"
25 dram glass vial with a closed bottom poly cap. Punch or cut a snug hole
in the bottom (inside) of the cap, shove the stub pin in and place in
vial...

Regards,
Woody

Woody White
BWXT Services:
http://www.bwxt.com/bwxt.html
My Site:
http://woody.white.home.att.net

-----Original Message-----
} From: Giles, Bill [mailto:William.Giles-at-TIMET.com]
Sent: Monday, August 02, 2004 1:39 PM
To: 'Microscopy-at-microscopy.com'

Hey Listers,

We are looking for a source of individual metallographic sample storage
containers. Our mounts are typically 1" diameter and 0.5-1" tall.

Anyone have a source for these?

William Giles
Senior Electron Microscopist
Metallography Lab Coordinator
P.O. Box 2128
Henderson, Nevada 89009

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (castrj01-at-endeavor.med.nyu.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, August 10, 2004 at 07:38:02
---------------------------------------------------------------------------

Email: castrj01-at-endeavor.med.nyu.edu
Name: George Castro

Organization: New York University Medical Center

Title-Subject: [Microscopy] [Filtered] siemens elmiskop 1a

Question: }
} I am writing in the hope that
} you might refer us to someone able to repair and service a Siemens
} Elmiskop 1A. I have obtained a limited amount of contacts through the
} web, but with no knowledge of this scope because of its age. Perhaps
} among your members there is a better chance.
} I am very grateful for your time and attention.
} George Castro
}
} George Castro
} Dept. of Surgery
} New York University Medical Center
} 520 First Ave. #421
} New York, NY 10016
} Tel 212 263 6777
} fax 212 263 0227
} castrj01-at-popmail.med.nyu.edu
}

---------------------------------------------------------------------------

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Hi,

Since the end of last year the idea of a Human Cytome Project has been
discussed at several meetings. At the F.O.M. meeting
(http://www.focusonmicroscopy.org/2004/program.html) this year the idea
of a Human cytome Project was discussed in public for the first time.

The next occasion will be the European Microscopy Meeting
(http://www.emc2004.be/) in Antwerp at the end of this month (Friday
August 27, Session LS 18. Round table: The Human Cytome project).

I hope it will be an interesting discussion and I look forward to meet
you at the meeting.

An interesting article on the idea of a Human Cytome Project:
Cytomics - New Technologies: Towards a Human Cytome Project
Valet G., T�rnok A.
Cytometry 59A:167-171 (2004)

Some links on the topic:

http://www.biochem.mpg.de/valet/cytompr1.html
http://www.biochem.mpg.de/valet/cytompr2.html
http://ourworld.compuserve.com/homepages/pvosta/humcyt.htm
http://www.cytomics.info/

Regards,

Peter Van Osta

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Dear Gerald,
I have found that Abe Dayani of Refining Systems Inc. gives good prices and
service on sputtering and deposition targets of all types. He specializes in
targets. You can contact him at:
Refining Systems Inc.
PO Box 72466
Las Vegas, NV 89170
phone: 702-368-0579, fax: 702-368-0933
www.refiningsystems.com
I am just a satisfied customer.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "Gerald Bourne" {grb-at-ufl.edu}
To: "Microscopy ListServer" {Microscopy-at-msa.microscopy.com}
Sent: Friday, August 06, 2004 8:05 AM

Article in today's New York Times about joys of microscopy for children
etc. If the link is still up:
http://nytimes.com/2004/08/10/science/10essa.html?8hpib

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/

{/x-flowed}

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Hello Everyone
We had a wonderful time in Savannah at the M&M meeting. Many thanks
to all the organisers, vendors and particpants who made it so
worthwhile for me.

But one piece of news I heard at the meeting was that Spurr resin is
supposed to be going off the market next year. As far as I understand
it one of the ingredients is being removed. Has anyone got any
comments about this or heard of an alternative ingredient?
Elaine

--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca

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George,
Peter Stolzenberg of Pesto Inc. had serviced the Siemens in the past but I
received word after I returned from the M&M 2004 meeting that he had recently
passed away. He was the serviceman for my Philips 200 and I received the
message so quickly because I had requested that he do a routine for me when I
returned. Consequently I am also looking for someone who can help me service my
TEM if I get stuck.

A few years ago there was a serviceman working for JEOL whom I had first met at
Temple University when he was servicing a Siemens Elmiskop there. Unfortunately
I can not remember his name.

Pat Connelly
Univ. of PA, Biology
Philadelphia, PA 19104-6018
psconnel-at-sas.upenn.edu
=========================
} Organization: New York University Medical Center
} Title-Subject: [Microscopy] siemens elmiskop 1a
} } I am writing in the hope that
} } you might refer us to someone able to repair and service a Siemens
} } Elmiskop 1A. I have obtained a limited amount of contacts through the
} } web, but with no knowledge of this scope because of its age. Perhaps
} } among your members there is a better chance.
} } I am very grateful for your time and attention.
} }
} } George Castro
} } Dept. of Surgery
} } New York University Medical Center
} } 520 First Ave. #421
} } New York, NY 10016
} } Tel 212 263 6777
} } fax 212 263 0227
} } castrj01-at-popmail.med.nyu.edu

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Dear Listers,

I am just catching up after a holiday so apologies for any delay. There has
been a lot of interesting info regarding early Leitz and Reichert
ultramicrotomes. I can add a little more to this dialogue.

During my time with Reichert-Jung UK as EM product specialist I was offered
several old ultramicrotomes before they were to be thrown into the Skip
(Dumpster in the US?). At one time I had around a dozen of these dinosaurs
cluttering up my house but most of them are now thankfully residing in the
Archive Building of the Science Museum in London.

Some of those I had were as follows:

Sorvall MT1 and MT2
Reichert Om U1 a design by Prof. H Sitte taken up by that company
LKB Sjostrand - the original ultramicrotome from LKB on which I learned
ultramicrotomy
Leitz - after a design by Fernandez-Moran, a massive but beautifully made
unit
Philips - yes even this company dabbled in ultramicrotomes
Cambridge Rocker - a factory modified microtome for ultra thin sectioning
Cooke and Perkins - a simple English ultramicrotme from the fifties
Cambridge Huxley
Si-ro-flex - an excellent microtome with superbly novel and advanced
features made by the Fairey Aviation in Australia

For those interested the first attempts to cut "ultrathin" were a bit of a
cheat as the idea was to cut cake-type slice from a specimen and try to
image cells at the thinnest part of the wedge. Not entirely successful.
There was an early diversion with high speed microtomes in the forties
particularly in the USA with massive rotational speeds up to 57,000 rpm but
cost and probably aerodynamics (or lack of them) led to there demise.

For anyone who might be as 'barmy' as I am with the history of these things
I was once talked into writing a short article for "Microscopy and
Analysis". The reference is:

The Thin End of The Wedge - A personal View of The Development of The
Ultramicrotome by T W Cooper, Microscopy and Analysis, January 1990.

I have no electronic copy of this dry old missive, but for anyone unable to
track it down I do still have a few reprints available that I would happily
send one to any interested party,

Best regards

Terry Cooper
TAAB Laboratories Equipment Ltd
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England
Tel ++44 (0)118 981 7775 Fax ++44 (0)118 981 7881
e-mail sales-at-taab.co.uk
www.taab.co.uk

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So sad to hear of Peter Stolzenberg's passing. He lived and had his
offices in Cold Spring Harbor, NY (on Long Island) when I was a grad
student at the CW Post campus of LIU, and he kept our ancient Hitachi
Hu-11A in fine running order. I got to know Peter quite well during
the protracted period of time it took to track down one circuit in
the HV cabinet that would short out in a very unpredictable fashion.
It was always fun to be working at the scope and hear what sounded
like a crack of lightening followed by total loss of HV. It was a
matter of having Peter there with his meters in the right place at
the right moment. Since his home was just a few miles down the road
from the campus, he would stop by in the morning on his way out to
other calls and then again on his way home at the end of the day.
This went on for about 2 weeks or so. We were finally lucky, he
found the problem and even had the right tube (yes, it was all vacuum
tubes, remember those?) in stock.
I learned a lot of what I know about the inner workings of an EM from
Peter, and with his coaching and coaxing, got past my uneasiness
about tackling mechanically-related problems with that old beast.
Skills that I've carried with me.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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Terry's mention of the high speed microtomes reminded me of a talk by
the then semi-retired Keith Porter that I was lucky enough to attend
in which he discussed the early years of EM work at the Rockefeller
University. One of the things he described was just such a
microtome. He said that the cutting speed was very fast, and that
the sections flew off the knife ( steel, if I remember correctly) and
were caught against a wire screen cage surrounding the microtome.
The sections were manually retrieved from the screen.
I hope I've remembered this correctly. Dr. Porter's description was
so animated, it created a very vivid impression.
Talk about doing things the hard way!

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

{/x-flowed}

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Good Morning Elaine and all other microscopy listservers whom are
interested in the Spurrs resin and its "whereabouts". Although it is
true that one of the constituents is being dwindled from the market
ERL-4206 (due to safety issues) for the past 2 years all of our
Scientific team at Electron Microscopy Sciences has been doing
comparitive testing of like and similar resins to find the one that best
works as the original. Over the 2 years of testing we have been able to
come up with one and modify it to the point where it is basically
undistinguishable from the original 4206. We call it the ERL
4221(catalog number EMS 15004). Formulas do not have to change and the
4221 will become an exact replacement fo the 4206 and none of us will
ever know the difference. Blocks, sectioning, staining all will remian
unchanged. So this is the good news. At this time there is no bad news
at all.
If you have any questions or I may be of any assistance please do not
hesitate to contact us. We look forward to hearing from you

Sincerely

Stacie Kirsch
Electron Microscopy Sciences
Tel: 215-412-8400
Fax: 215-412-8450
E-mail: sgkcck-at-aol.com
Website: www.emsdiasum.com
-----Original Message-----
} From: Elaine Humphrey [mailto:ech-at-interchange.ubc.ca]
Sent: Tuesday, August 10, 2004 3:34 PM
To: microscopy-at-msa.microscopy.com

Hello Everyone
We had a wonderful time in Savannah at the M&M meeting. Many thanks
to all the organisers, vendors and particpants who made it so
worthwhile for me.

But one piece of news I heard at the meeting was that Spurr resin is
supposed to be going off the market next year. As far as I understand
it one of the ingredients is being removed. Has anyone got any
comments about this or heard of an alternative ingredient? Elaine

--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca

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Oh, I'm sorry to hear that! Mr. Stolzenberg was just here a couple of
weeks ago to service our old Zeiss EM 10-he did a great job. Please
send my condolences.

Does anyone know of anyone who can service our Zeiss EM 10CA? It's OK
now, but we will certainly need someone in the future.

Thanks,
Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Dept. of Pharmacology & Toxicology
Ernest Mario School of Pharmacy
Rutgers University
41 B Gordon Rd
Piscataway, NJ 08854

psconnel-at-sas.upenn.edu wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

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Colleagues

I have spoken with Eric Ambrose concerning this message. In my opinion it
is not an advertisement and is definitely of interest and importance to the Microscopy Community.

Eric , thank you again for first checking with me on this.

Nestor
Your Friendly Neighborhood SysOp

----------------------------------------------------

Bill:

We have some containers that are ideal for this application. You can
see an image of them on our website at www.southbaytech.com. Do a
keyword search for "containers". You will also find them listed with
our extensive range of metallographic consumables under the
"consumables" button. I will contact you off-line with pricing.

Best regards-

David

Giles, Bill wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
David Henriks
Vice President

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.

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Hello Microscopy folks,
I am trying to resurrect an old spectroscopy system that uses a Tracor
Northern TN-6500 box for controlling a linear diode readout. Anyone out
there with a manual for the TN-6500? I am happy to pay photocopy charges
or do anything (reasonable!) to get my hands on one. Thanks again.
Greg Mulhollan
-----------------------------------
Gregory Mulhollan, Ph.D.
Saxet Surface Science
1001 S. Sunset Canyon Drive
Dripping Springs, TX 78620
(512)858-2841 office
(512)694-4879 cell
mulhollan-at-saxetsurfacescience.com
www.saxetsurfacescience.com

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

William Giles wrote:
============================================================================
==============
We are looking for a source of individual metallographic sample storage
containers. Our mounts are typically 1" diameter and 0.5-1" tall.

Anyone have a source for these?
============================================================================
==============
SPI Supplies has manufactured for some years our special mount storage box
for 1" mounts, see URL
http://www.2spi.com/cataelog/boxes/speci_box.shtml

This would be our SPI #02020-AB and each one holds eight mounts. There are
inserts for desiccating capsules. And once the samples are loaded into the
box, and the lid closed, some ordinary Scotch Tape is put around the lip of
the closed box to seal out air or anything else that might seep in and
damage samples.

Disclaimer: SPI Supplies is the manufacturer of this special kind of
storage box.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

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Thanks for all the responses; I'll filter thru all the suggestions.

William Giles
Senior Electron Microscopist
Metallography Lab Coordinator
P.O. Box 2128
Henderson, Nevada 89009
(702) 566-4436
(702) 564-9038 (Fax)
William.Giles-at-timet.com

*

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We use the "Cap Plugs" Woody has suggested and I find they work well if
you're looking for inexpensive (a few cents per piece) individual sample
storage.

The caps come in a variety of shapes and sizes, and I'm sure there are
many sources for such items. The vendor we ordered from has a good page
with drawings and dimensions for each:
http://www.niagaracapsandplugs.com/nonthreaded_caps.htm

Regards,

Steven T Szewczyk
Materials Science Contractor
US Army Research Lab
Aberdeen Proving Ground, MD

-----Original Message-----
} From: White, Woody N. [mailto:nwwhite-at-bwxt.com]
Sent: Monday, August 02, 2004 4:08 PM
To: 'Giles, Bill'; 'Microscopy-at-microscopy.com'

Let us know if you find something that is suitable and *inexpensive*.
Microscopy supply vendors sell plastic boxes, but at the rate I go
through samples, I would go broke and run out of storage room as well.

No inexpensive vials, jars, etc. that I have found protect the prepared
surface well - no mounting method. The best compromise is to use a
snugly fitting "Cap Plug" (poly closure used to close pipe ends, other
holes) and partially insert the mount into it.

If nothing else fits, I stuff the large mounts into a locking closure
plastic bag. It is likely that if fragile or coated, this will harm the
specimen, however.

A great solution (not my idea) for 1/2" (12mm) pin-mounts is an
"Opticlear" 25 dram glass vial with a closed bottom poly cap. Punch or
cut a snug hole in the bottom (inside) of the cap, shove the stub pin in
and place in vial...

Regards,
Woody

Woody White
BWXT Services:
http://www.bwxt.com/bwxt.html
My Site:
http://woody.white.home.att.net

-----Original Message-----
} From: Giles, Bill [mailto:William.Giles-at-TIMET.com]
Sent: Monday, August 02, 2004 1:39 PM
To: 'Microscopy-at-microscopy.com'

Hey Listers,

We are looking for a source of individual metallographic sample storage
containers. Our mounts are typically 1" diameter and 0.5-1" tall.

Anyone have a source for these?

William Giles
Senior Electron Microscopist
Metallography Lab Coordinator
P.O. Box 2128
Henderson, Nevada 89009
(702) 566-4436
(702) 564-9038 (Fax)
William.Giles-at-timet.com

*

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Hello Microscopists !

Quite some time ago, in anticipation of a congenial meeting of interested
parties at a common-ground investigation of several broken steel bolts in
the Cleveland, Ohio, area, I asked about suitable SEM labs in the area.
I received an excellent response from The List, which indicates that it is
the primary means of rapid and effective communication in the micrsocopy
community. Alas, there was a slower response from the above-referenced
interested parties, and so no agreement about protocol or venue was
reached until we had all retired to our own offices. Later on, we wound up
going back to the place selected originally by one of the parties way back
at the beginning of the project, where 1000X is about the limit of useful
magnification. I am sure any one of you could have done better.

Thanks to all who reponded.

Best regards,
George Langford, Sc.D.
Principal Consultant
Amenex Associates, Inc.
amenex-at-amenex.com
http://www.amenex.com/

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Mr. Chapman,
When you say "stock ammonia solution", are you talking about standard
Ammonia(NH3)?

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (garyeaston-at-scannerscorp.com) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, August 3, 2004 at 13:34:53
---------------------------------------------------------------------------

Email: garyeaston-at-scannerscorp.com
Name: Gary M. Easton

Organization: Scanners Corporation

Title-Subject: [Microscopy] [Filtered] Cambridge/Leica/LEO S360 SEM

Question: I just picked up a used Cambridge S360 that gives me an
error code 307(defective LAB6 switch). Does anyone out there that
has a complete list of the software error codes and their
explanation? The operator's manual offers no help. Also, if anyone
has the setup software (mag cal,
etc) for this series SEM and is willing to part with a copy, it would
be greatly appreciated. Please reply offline to
garyeaston-at-scannerscorp.com. Thanks in advance.

Gary M. Easton

---------------------------------------------------------------------------

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cnorton-at-wis-inc.net) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, August 3, 2004 at 15:36:41
---------------------------------------------------------------------------

Email: cnorton-at-wis-inc.net
Name: chris norton

Organization: Wafer Inspection Services, Inc.

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: We are searching for service engineers with KLA-Tencor
service and applications experience on all models.
Prometrix UV1250/1280; RS 75; 6420; AIT; Starlight; 8100 CD SEMS; etc.

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Hi all
I am looking at Leica ultra-microtomes. The new version (the UC6b) has
two controllers, the key pad or the touch screen. I would appreciate
any input or comments on these. I am especially interested in reasons
to get one or the other.
Thanx
David

_____________________

David Elliott Ph.D.
Research Assistant Professor
Department of Cell Biology and Anatomy
PO Box 245004
Tucson, AZ 85724

Voice: 520-626-7870
Fax: 520-626-2097

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Hi all, I am co-chair of a workshop being given on algae identification at
the North American Lakes Management Society International Symposium in
Victoria Canada. The workshop is being offered November 2,
2004. Normally, its not a problem to get the scopes, but I can't find a
source in Canada. I need 10 teaching scopes with Phase and 400 objectives
and 1 BX60 with Phase at 200 and 400 with a trinoc head and a digital
camera. Can anyone help? I'm located in Michigan, United States. Thanks
much, Ann.

Ann St. Amand, Ph.D.
President
PhycoTech, Inc.
620 Broad St., Suite 100
St. Joseph, Michigan 49085 USA

269-983-3654 (voice)
269-983-3653 (fax)
mailto:astamand-at-phycotech.com
www.phycotech.com

Specializing in Aquatic Sample Analysis and Microscope Accessories

Director, Region V, North American Lake Management Society, www.nalms.org

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Hi David
Keypad does not have all features, touch screen has. It's quite
confusing. For instance if you go with touch pad, you need to buy $5K
interface (whichever it called) for their new cryo-attachment and so on. As
far as I understand, touch-screen is sort of "standard" and they "invented"
key-pad to make cheaper version of the ultratome. You need to check the
compatibility issue, because UC6 is not compatible with previous generation
stuff, like UCT, FSC etc. Good luck with shopping. Sergey

At 08:09 AM 8/4/2004, you wrote:

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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-089
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (matthew.salanga-at-childrens.harvard.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html#form
on Wednesday, August 4, 2004 at 15:15:22
---------------------------------------------------------------------------

Email: matthew.salanga-at-childrens.harvard.edu
Name: Matthew Salanga

Organization: Children's Hospital Boston

Education: Graduate College

Location: Boston, MA

Question: Greetings!

Does anybody know where I can obtain CFP-YFP FRET control slides. Or
transfected a stable FRET positive cell line. Ideally I am looking
for cells grown on coverslips which have tagged CFP and YFP proteins
that are known to elicit a FRET response.

Thanks!

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sspence-at-flemingc.on.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Wednesday, August 4, 2004 at 19:41:48
---------------------------------------------------------------------------

Email: sspence-at-flemingc.on.ca
Name: Susan Spence

Organization: Tottenham Public School

Education: 6-8th Grade Middle School

Location: Tottenham, Ontario Canada

Question: I have a very ruly group of grade 8's, very few
microscopes, and even fewer quality slides from which to view
organelles and unicellular organisms. DO you have any suggestions as
to where I may find good images, either from a light microscope or an
electron microscope? I want to show them what cells really look like
through a microscope without actually having them to touch one.
Thanks for your suggestions!

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The Leica ultra-microtome ....?!
This brings back memories of the original one, based on the design by
Humberto Fernandez-Moran about 50 years ago. Anyone still remember it?
Its design was absolutely unique in that the specimen chuck was mounted
in a motor-driven massive cylinder which performed complete rotations in
the horizontal plane over two pairs of huge flat sapphire bearings
placed in a "V" orientation. I imagine that it must have been a "cow of
a thing" to set up! It was possibly developed to utilize the newly
invented diamond knives (also invented by F-M, whose genius contributed
so much to new developments in the early days of TEM). I only ever saw
advertising brochures of the ultra-microtome, so would be interested to
learn of users' experiences from those times and perhaps also something
about the fate of these now-historic instruments.

-----Original Message-----
} From: David Elliott [mailto:David.Elliott-at-yale.edu]
Sent: Wednesday, August 04, 2004 5:09 PM
To: Microscopy ListServer

Hi all
I am looking at Leica ultra-microtomes. The new version (the UC6b) has
two controllers, the key pad or the touch screen. I would appreciate
any input or comments on these. I am especially interested in reasons
to get one or the other.
Thanx
David

_____________________

David Elliott Ph.D.
Research Assistant Professor
Department of Cell Biology and Anatomy
PO Box 245004
Tucson, AZ 85724

Voice: 520-626-7870
Fax: 520-626-2097

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I do remember setting up something like that. Except my recollection
is that it was designed by Sjostrand. The cylindrical chuck unit had
a groove around it so that you would mount the motor on the wall, and
run a long V-belt from the motor to the microtome. This was to
minimize vibration. The other feature that I remember is that the
cutting speed was quite fast, not at all like what we use today.

Joel

I am using a Reichart-Jung MT 6000 ultramicrotome for standard EM
sectioning. After the specimen goes through the cutting range there is a
beep and the microtome electronics will freeze up, I have tried reseting the
knife and specimen advancements but the problem still persists, does anyone
have a recommendation?

Thanks,

Todd Hamm
Research Technician
Oklahoma Medical Research Foundation
tmhamm09-at-yahoo.com

_________________________________________________________________
Express yourself instantly with MSN Messenger! Download today - it's FREE!
http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/

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On my search for the possibility to get high tension and magnification
values out of older Philips and Zeiss TEMs I got no answer from the group
and finally found after an extensive search of the web the following
interface-cards for these TEMs:

For Philips EM 400, 410, 420: http://www.stefan-diller.com/rem_tem3_en.htm
For Zeiss TEMs EM 10 A, B and C: http://www.stefan-diller.com/rem_tem4_en.htm

I hope this is useful for somebody having the same problems with wrong
mag-values and doing new reference images after changing the HT-value on an
old TEM...

--
Timo Junker Holografie
Lindenstr. 10
97297 Waldb�ttelbrunn
Tel: ++49 (0)931 4520655
Fax: ++49 (0)931 4520657
www.holografie.com

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Colleagues

The July Archives are now on-line at

http://www.microscopy.com/MicroscopyListserver

Nestor
Your Friendly Neighborhood SysOp

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Hi All,

We have a Reichert Jung Ultracut microtome in need of a part,
specifically the clutch assembly in the fine advance. If anyone could
tell us where we could get such a part it would be greatly appreciated.
In Australia would be best but we'll get it from any part of the globe
if we have to!

Thank you very much for your help and it's great to be back working in
microscopy again after a few years "sabbatical" on other projects!!

Cheers

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.

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Colleagues

I have spoken with Eric Ambrose concerning this message. In my opinion it
is not an advertisement and is definitely of interest and importance to the Microscopy Community.

Eric , thank you again for first checking with me on this.

Nestor
Your Friendly Neighborhood SysOp

----------------------------------------------------

Hi,

For the M & M Education Outreach program next year in Hawaii, we would
like to gather information on the various curriculum available for
teaching electron microscopy in the high schools. One of the drawbacks
of doing this, is that each of us must develop exercises and curriculum
to make it available to the high school teacher. This is very time
consuming. Rather than re-inventing the wheel, we are trying to find
those involved in these types of outreach activities who would be
willing to share some of their materials or let us know if it is
available for purchase. Even finding out the logistics of how you teach
the courses in the high school would be helpful.

This would be really helpful to percolate the excitement about
microscopy to high school students.

You can email me off-line.

Thank you so much in advance, for any assistance you can be.

Judy Murphy
Stockton, CA

murphyjudy-at-comcast.net

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 13 11:29:01 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (morillm-at-mail.rockefeller.edu) from http://microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, August 13, 2004 at 11:26:17
---------------------------------------------------------------------------

Email: morillm-at-mail.rockefeller.edu
Name: Marivel Morillo

Organization: Rockefeller University

Title-Subject: [Microscopy] [Filtered] full-time electron microscopist position open

Question: Attention all Electron Microscopists!

The Laboratory of Developmental Genetics at The Rockefeller University
seeks a full-time electron microscopist to assist in studies of nervous
system development and cell death in the nematode C. elegans.

Work will involve preparing specimens for TEM, serial sectioning, and 3-D
image reconstructions. The successful candidate will become an integral part
of ongoing scientific efforts in the lab, and could also pursue independent
research. Previous experience with TEM is required, and experience with
serial sectioning experience a plus. Applicants must hold a minimum of a
Bachelor's degree.

We offer a competitive salary, comprehensive benefits, a gracious working
environment, and tuition reimbursement. For immediate consideration, send
resume/C.V. to: Ms. Kara R. Marshak, Senior Employment Specialist, The
Rockefeller University via fax (212)-327-7079 or email
{mailto:marshak-at-rockefeller.edu} marshak-at-rockefeller.edu. For more information about ongoing research in
this laboratory, please visit our website located at
{http://www.rockefeller.edu/labheads/shaham/shaham-lab.php} http://www.rockefeller.edu/labheads/shaham/shaham-lab.php.

An Affirmative Action/Equal Opportunity Employer. The Rockefeller
University appreciates all responses and will contact candidates selected
for further consideration.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Sat Aug 14 02:15:49 2004

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I have just received a box full of wax blocks I made about 18 years ago for a study of reproductive biology and zooxanthellae of Millepora platyphylla, fire coral. I don't have a microtome, but thanks to the generosity of a microscopist cleaning out a lab, I have a steel microtome knife. I surely cannot afford a microtome, or especially the shipping, but I remember some plans from some discarded library material some years ago, for microtomes. One used a candle to heat a long metal rod, effectuating a gradual lengthening, for example.

Can anyone suggest whether hand sections are worth while to try of wax blocks. The blocks were made with paraplast. Being several years old, even though I have to assume they are still ok, should I take any special steps with storage or rejuvenation?

I'm afraid to ruin them. The study broke some ground, has not been published, and deserves to be followed through. Even though my notes have been lost through years of sore neglect, typhoons, and ex-spousal neglect, there are some hopeful clues in these specimens that would still be worthwhile, and dates of collection and other information are still recoverable for the most part, through existence of a parallel labelled collection of hard parts, with dates written on them. So... any suggestions on long term care of wax blocks, as well as suggestions for hand-sectioning, would be welcome.

I have a hand microtome, with German inscriptions, that may be useful.

Thank you,

Alan Davis
Kagman High School
Saipan, N. Mariana Islands
aedavis-at-eccomm.com

From MicroscopyL-request-at-ns.microscopy.com Sat Aug 14 15:55:53 2004

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Alan,

Having made more Paraplast embeddings than I care to remember..... The only
thing I can think of is to keep them in a cool dry place; otherwise, they
should last a very long time. The other question about how to section is an
interesting one. You already have a hand microtome and it might be worth a
try although I don't know how it is to be set up. You have a microtome
blade but do you have a handle for it? If not, it should be a low cost item
to acquire and I have the name of a local place that may have some.

I made a hand microtome using a screw type micrometer gauge, cutting off the
arch portion and mounting the screw portion to a steel cylinder with a hole
in it through which the screw part would push the tissue sample out by a
known amount. The top of the cylinder of course was ground to a reasonably
fine polish. The exposed sample was then cut using a microtome blade on a
handle. I used it primarily for fresh plant samples that I would sandwich
between some soft material like balsa, or Sambucus pith, cork, etc before
inserting it into the hole.

Damian Neuberger Ph.D.

I have just received a box full of wax blocks I made about 18 years ago for
a study of reproductive biology and zooxanthellae of Millepora platyphylla,
fire coral. I don't have a microtome, but thanks to the generosity of a
microscopist cleaning out a lab, I have a steel microtome knife. I surely
cannot afford a microtome, or especially the shipping, but I remember some
plans from some discarded library material some years ago, for microtomes.
One used a candle to heat a long metal rod, effectuating a gradual
lengthening, for example.

Can anyone suggest whether hand sections are worth while to try of wax
blocks. The blocks were made with paraplast. Being several years old, even
though I have to assume they are still ok, should I take any special steps
with storage or rejuvenation?

I'm afraid to ruin them. The study broke some ground, has not been
published, and deserves to be followed through. Even though my notes have
been lost through years of sore neglect, typhoons, and ex-spousal neglect,
there are some hopeful clues in these specimens that would still be
worthwhile, and dates of collection and other information are still
recoverable for the most part, through existence of a parallel labelled
collection of hard parts, with dates written on them. So... any
suggestions on long term care of wax blocks, as well as suggestions for
hand-sectioning, would be welcome.

I have a hand microtome, with German inscriptions, that may be useful.

Thank you,

Alan Davis
Kagman High School
Saipan, N. Mariana Islands
aedavis-at-eccomm.com

From MicroscopyL-request-at-ns.microscopy.com Sun Aug 15 10:31:27 2004

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Hello everybody
�� I am looking for advice on representing 2D and 3D vector data. Namely, the image I collect from the microscope is either two dimensional vector (dx,dy) at each point, or three dimensional vector (dx,dy,dz). For example, it is in-plane polarization (or magnetization) vector or full 3D polarization vector. The data is collected in the form of ASCII files.
�� I have seen numerous examples in which such data is represented as color-coded images (e.g., EBSD) and I will appreciate advice on how it can be done best (e.g., how to relate (dx, dy) and RGB color intensities, etc). I am currently using the straightforward approach of importing data files into Mathematika (they are generated by custom LabView based software) and assigning color values using RGBColor function.
�� Thank you in advance
�� Sergei

--
Sergei V. Kalinin,
Wigner Fellow and Research Staff Member
Oak Ridge National Laboratory,
1 Bethel Valley Rd,
Bldg. 3025, MS6030,
Oak Ridge, TN 37831
Phone: (865) 241-0236
FAX: (865) 574-4143
URL: sergei2.kalininweb.com
New: http://nanotransport.ornl.gov

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 16 07:17:48 2004

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NIST, AVS and MAS will co-sponsor a Workshop on Modeling Electron Transport
for Applications in Electron and X-ray Analysis and Metrology at NIST
(Gaithersburg, Maryland, November 8-10, 2004) to highlight progress and
determine directions for future development of electron-simulation
techniques and the needs for electron-interaction data. This Workshop will
bring together experts in the physical theory and data that support the
simulation techniques as well as scientists and engineers who develop
models and obtain results for specific applications. Applications will
include x-ray microanalysis of particles, rough and layered surfaces, Auger
analysis of near-surface features, testing of matrix-correction procedures
for bulk analysis, metrology of sub-micrometer scale features in SEM
images, low-voltage and ultra-low-voltage microscopy simulation, radiation
physics, etc. A planned poster session will include demonstrations of
relevant software and databases.

Further information on the Workshop can be found at
http://www.nist.gov/electron. We are now inviting the submission of
abstracts for the Workshop, the deadline being October 8. Enquiries should
be directed to dale.newbury-at-nist.gov

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 16 09:38:32 2004

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Hello, I work for a small research lab, and we plan to do TEM on mouse
tissue. We will fix the tissue in Trump's which is 4% formaldehyde + 1%
glutaraldehyde in a phosphate buffer. Has anyone use this fixative before?
Any pitfalls that we should avoid? Any comments will be very helpful.
Thanks alot!

Scott
Beth Israel Deaconess Medical Center
Boston, MA

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 16 12:01:15 2004

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} Good Morning -
}
} There is a great job opening in my area. The work is very interesting and
} the work environment is casual.
} Thousand Oaks (T.O.) is 1 hour NW of LA in beautiful Ventura County. The
} hiking trails are everywhere and we are 8 miles north of Malibu Beach.
} T.O. was recently rated safest city over 100,000 in the US. If interested
} you can email me directly or go thru the Amgen website and click on
} careers.
regards
} Gianni Torraca
} 805-447-7445
} gtorraca-at-amgen.com
}
} Title: Research Scientist/Assoc Scientist
} Department: 3760 - Analytical Sciences
} Location: Thousand Oaks CA
} Job ID: amge-00004427
} HR Recruiter: Ernest Allen
} (805) 447-6072
} ernesta-at-amgen.com
}
} Requirements:
} Strong skills in microscopy and micro-particle handling are needed.
} Experience in optical microscopy, FT-IR microscopy, and SEM-EDS is
} preferred. Experience in micro-particle identification and sizing and
} manufacturing incident investigation is desired. Experience in techniques
} commonly used for PSDA, such as light-obscuration techniques, as well as
} other skills relevant to the structural characterization of
} pharmaceuticals or biopharmaceuticals, is a plus. Excellent problem
} solving skills and good written and verbal communication skills are
} required.
} Educational requirements:
} M.S. or equivalent with extensive experience in Physical Chemistry,
} Analytical Chemistry, Biochemistry or related field.
}
} Job Summary:
} This position is for a scientist in the structural characterization group.
} A key responsibility will be analytical support of investigations by
} identification of adventitious micro-particles. Support of process
} development and support of incident investigations for both clinical and
} commercial drug products will be required. This position requires hands on
} work in the lab and may require supervision of scientific staff as the
} group grows. The position will also involve some particle size
} distribution analysis of resins, inclusion bodies, and similar objects.
} Experience:
} Relevant experience in the pharmaceutical or biopharmaceutical industry is
} desirable; other relevant experience may be acceptable. Experience in
} development and implementation of emerging technologies is desirable, and
} supervision of scientific staff is a plus.
}
****************************************************************************
**********************

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 16 13:59:47 2004

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Phosphate fixes can result in fine calcium precipitates. I prefer HEPES or
PIPES.

At 10:59 AM 08/16/04 -0400, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 16 21:10:56 2004

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I use modified Trump's fixative with 0.1M sodium cacodylate buffer
without problems with most kinds of tissue. I also use 0.1M phosphate
buffer without artifact problems as long as I wash in buffer 3X after
fixation prior to osmification (1% osmium in 0.1M buffer for 60 minutes)
followed by 3X rinse in buffer and additional 3X rinse in water prior to
dehydration in ethanol series. My problem had been "osmium peppering" -- a
fine precipitate contaminating samples run up in phosphate buffer when not
thoroughly washed. Na-cacodylate avoids this problem, but I always rinse
thoroughly when running up samples for TEM.
I run up tissue at room temperature because of a superstition
acquired as a graduate student that cold temperature promotes the
disassembly of microtubules. Does anyone have thoughts pro or con about
running up tissue on ice vs. room temperature?

Dean Abel
Biological Sciences 143 BB
University of Iowa
Iowa City IA 52242-1324

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 16 21:22:24 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (frah0010-at-umn.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, August 16, 2004 at 19:54:57
---------------------------------------------------------------------------

Email: frah0010-at-umn.edu
Name: Ellery Frahm

Organization: University of Minnesota Electron Microprobe Lab

Title-Subject: [Microscopy] [Filtered] MListserver: Cameca timeline question

Question: Greetings,

Does anyone know approximately when Cameca SU30 SEMprobes were manufactured?

Thanks,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 17 02:07:43 2004

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Dear all,

I'm looking for a staining method to prepare an ultramicrotomed
polyurethane polymer for a TEM investigation. Contrast is needed between
the hard and soft segements of the polymer. The functional groups of the
soft segments are mainly carbonates, and of the hard segments amides or
urea. Both segments also have a different urethane content.

Are there staining methods recommended for polyurethane polymers with
such a combination of functional groups (for certain urethanes, OsO4 or
RuO4 may be used)? Can such polymers be imaged by coherent phase
contrast alone?

Thanks in advance

Thomas Keller

--
---------------------------------------------------
Dr. Thomas Keller
Institute of Materials Science and Technology (IMT)
Friedrich-Schiller-University Jena
L�bdergraben 32
D-07743 Jena
Germany
Phone: ++ 49 3641 947742
Fax: ++ 49 3641 947732
Mobile: ++ 49 170 1439522
Internet: t.keller-at-uni-jena.de
---------------------------------------------------
Visit us under http://www.uni-jena.de/matwi/

"Making Materials Science Work 4 U"
---------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 17 09:33:17 2004

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Dean,
I have used a combination fixative of 1% glutaraldehyde,1% OsO4 in
0.05M Phosphate Buffer -at-6.2pH ON ICE and kept dark for 45min. for
many tissues. There are many microtubules present.
Perhaps the microtubules were stablized by the osmium before
they were able to disassemble in the cold temperature.

The only problem I had with this fixative was with bacteria inside
tissue cultured macrophages. It did not wash out as easily/quickly
as I expected. As a result I had the "peppering" of the osmium in and
around the bacteria itself when the TEM beam hit the bacteria.

Pat Connelly
Univ. of PA, Biology
Philadelphia, PA
psconnel-at-sas.upenn.edu
=======================
Quoting Dean Abel {dean-abel-at-uiowa.edu} :
} I use modified Trump's fixative with 0.1M sodium cacodylate buffer
} without problems with most kinds of tissue. I also use 0.1M phosphate
} buffer without artifact problems as long as I wash in buffer 3X after
} fixation prior to osmification (1% osmium in 0.1M buffer for 60 minutes)
} followed by 3X rinse in buffer and additional 3X rinse in water prior to
} dehydration in ethanol series. My problem had been "osmium peppering" -- a
} fine precipitate contaminating samples run up in phosphate buffer when not
} thoroughly washed. Na-cacodylate avoids this problem, but I always rinse
} thoroughly when running up samples for TEM.
} I run up tissue at room temperature because of a superstition
} acquired as a graduate student that cold temperature promotes the
} disassembly of microtubules. Does anyone have thoughts pro or con about
} running up tissue on ice vs. room temperature?
}
} Dean Abel
} Biological Sciences 143 BB
} University of Iowa
} Iowa City IA 52242-1324

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 17 09:57:44 2004

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Hi, Thomas,

Specifically what information are you trying to obtain? If you are looking
for location of specific functional groups, you might try scanning
tunneling microscopy instead. I just sat in on a demo where Kim
Kangasniemi discussed some work like this, as part of his Master's
thesis. He can be reached at Kim-at-nt-america.com.

Hope this is helpful.

Barbara Foster
Microscopy/Microscopy Education
We've Moved!
313 S Jupiter Road, Suite 100
Allen, TX 75002
PH: 972-954-8011 FX: 972-954-8018 Web: www.MicroscopyEducation.com

~-at-~-at-~~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-
Optimizing Light Microscopy for Biological and Clinical Labs is available
in individual copies or classroom size orders. Visit
www.MicroscopyEducation.com
for details.
~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-~-at-

At 12:29 AM 8/17/04, Thomas Keller wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 17 11:14:03 2004

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Thomas
Depending on the size of "soft" and "hard" regions, the best way to image them can be variants of phase-sensitive AFM (phase mode), force modulation mode, or acoustic or ultrasonic force microscopy. Basically, all these techniques are sensitive to hardness and losses at the tip-surface junction; the variation is primarily in frequency range and modulation/detection mechanism. Resolution is primarily limited by the tip-surface contact area and can be as small as few nanometers. Please feel free to contact me directly if this sounds useful.
Sergei

--
Sergei V. Kalinin,
Wigner Fellow and Research Staff Member
Oak Ridge National Laboratory,
1 Bethel Valley Rd,
Bldg. 3025, MS6030,
Oak Ridge, TN 37831
Phone: (865) 241-0236
FAX: (865) 574-4143
URL: sergei2.kalininweb.com

-----Original Message-----
} From: Barbara Foster [mailto:bfoster-at-mme1.com]
Sent: Tuesday, August 17, 2004 1:16 PM
To: Thomas Keller; Microscopy-at-MSA.Microscopy.Com

Hi, Thomas,

Specifically what information are you trying to obtain? If you are looking
for location of specific functional groups, you might try scanning
tunneling microscopy instead. I just sat in on a demo where Kim
Kangasniemi discussed some work like this, as part of his Master's
thesis. He can be reached at Kim-at-nt-america.com.

Hope this is helpful.

Barbara Foster
Microscopy/Microscopy Education
We've Moved!
313 S Jupiter Road, Suite 100
Allen, TX 75002
PH: 972-954-8011 FX: 972-954-8018 Web: www.MicroscopyEducation.com

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Optimizing Light Microscopy for Biological and Clinical Labs is available
in individual copies or classroom size orders. Visit
www.MicroscopyEducation.com
for details.
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At 12:29 AM 8/17/04, Thomas Keller wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 17 21:27:50 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (NairIndira2004-at-yahoo.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, August 17, 2004 at 11:07:42
---------------------------------------------------------------------------

Email: NairIndira2004-at-yahoo.com
Name: Indira Nair

Organization: Bahauddin Zakariya University

Education: Undergraduate College

Location: Multan, Pakistan

Question: Dear Gurus,
can anybody help me to find any technical information on LEO EVO 50SXVP SEM? I've found description of EVO50XVP at LEO's site but no information on the former model.

Thanks in advance,
Indira

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 18 01:09:24 2004

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------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (NairIndira2004-at-yahoo.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, August 17, 2004 at 11:07:42
---------------------------------------------------------------------------

Email: NairIndira2004-at-yahoo.com
Name: Indira Nair

Organization: Bahauddin Zakariya University

Education: Undergraduate College

Location: Multan, Pakistan

Question: Dear Gurus,
can anybody help me to find any technical information on LEO EVO 50SXVP SEM? I've found description of EVO50XVP at LEO's site but no information on the former model.

Thanks in advance,
Indira

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 18 07:18:32 2004

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Trypan quenching of fluroescence has been used for years to decrease
fluorescence of particles and dextran. In our case, we are trying to
use it to quench the fluorescence of surface labeled particles to
distinguish internalized particles (phagocytosis) from external
particles. We have not gotten significant quenching, and as I read
the literature, I am unclear as to how this is supposed to work. In
the original Hed paper that most people reference, they use FITC
labeled particles and quench by putting the cells in a trypan
solution in a pH 4.5 buffer. They describe this as a FRET type
quenching to a non-radiative acceptor. Trypan does emit, but lets
leave this aside for now. Their explanation makes little sense to me
since with the known pH dependence of FITC, it seems to me they are
doing primarily pH based quenching as opposed to FRET based
quenching. If so, then are they simply looking at the difference in
FITC fluorescence between the phagosomal pH (5.5) and the external pH
(4.5)?
In other papers, rhodamine labelled particles are used, and trypan
quenching is also supposed to work, however in our case, we can only
see about 50% quenching. Given how opaque the solution is, I could
easily see that being a case of simply decreasing both excitation and
emission due to opacity of the solution. In some cases (yeast
particles) I have been told that the trypan actually binds to the
particles so that you can wash it out and the particles are still
blue, and in that case, I would imagine very different quenching
properties, since the trypan is no longer freely diffusing in
solution. I have yet to find literature that clearly investigates
the quenching phenomenon with different fluors under solution vs.
bound conditions. Can anyone help? Thanks- Dave
--

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd. U-3125
Storrs, CT 06269-3125
knecht-at-uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 18 11:54:06 2004

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Does anyone have an idea where I can find a NIST traceable glass scale that
measures in the range of 1 ~ 10 microns possibly with the first micron
subdivided into tenths. This will be used on a light microscope for
calibration purposes. Thank you

Robert Fowler
Quality Assurance Failure Analysis Technician
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.249
Fax: (770) 487-1460
email: robert.fowler-at-tdktca.com
www.component.tdk.com

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 18 13:24:30 2004

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Hello,

I'm looking at putting a digital camera (Evolution MP, QIMAGING
MicroPublisher 5.0 RTV) on my Olympus Stereozoom SZH Microscope (with DF
PLAN objectives). In the initial trial, I had some chromatic aberration.
I'm not sure where it's coming from. Does anyone have any experience with
combining this camera and microscope? Any suggestions to reduce chromatic
aberration?

Thanks,

Diane Ciaburri

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 18 15:16:57 2004

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Robert -

Not sure of the exact traceability path, but BCR and PTB are recognized
Worldwide.

http://www.irmm.jrc.be/rm/cert-reports/BCR-676_cert.pdf

Good Luck

Marc Helvey
Strategic Accounts Manager
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
Phone: (408) 428-1800, ext. 108
FAX: (408) 428-9555
E-mail: marc.helvey-at-vlsistd.com
Internet: http://www.vlsistandards.com

-----Original Message-----
} From: Robert.Fowler-at-tdktca.com [mailto:Robert.Fowler-at-tdktca.com]
Sent: Wednesday, August 18, 2004 10:16 AM
To: Microscopy-at-MSA.microscopy.com

Post Doctoral Position in the NCSU Analytical Instrumentation Facility SIMS
Laboratory

The Analytical Instrumentation Facility (AIF) of the NC State University
College of Engineering has an opening for a postdoctoral research associate
in its SIMS laboratory. The successful candidate will operate our magnetic
sector IMS-6f magnetic sector and our Atomika 4100 quadrupole SIMS
instruments. The successful candidate must be interested in learning and
participating in the analysis of a wide variety of samples (semiconductors,
metals, ceramics, polymers). Work in the laboratory will consist of a
combination of SIMS analytical technique research, experimental design,
instrument operation and data interpretation for university and industrial
researchers. Training in SIMS and other analytical techniques and
instrumentation (AFM, FIB, SEM, XPS, optical and stylus profilometry etc.)
will be provided as needed for supporting SIMS research and analysis. For
information on AIF, see our web site: http://www.ncsu.edu/aif/.

A Ph.D or equivalent in Analytical Chemistry, Applied Physics, Materials
Science or a materials related discipline is required. SIMS experience is
highly desirable although training will be given to a suitable applicant.
Experience in vacuum equipment and/or electronics maintenance; operational
and programming experience with Windows and Unix operating systems; and
experience with analysis of semiconductor or other non biological materials
are desirable.

Please send resume and three letters of reference to: Dr.
Dieter Griffis, Associate Director; Analytical Instrumentation Facility;
North Carolina State University; Box 7531, Room 118A EGRC; 2410 Main Campus
Drive; Raleigh, NC 27695-7531 or email dgriffis-at-ncsu.edu

NC State is an EOE/AA employer. NC State welcomes all persons without
regard to sexual orientation. ADA individuals desiring reasonable
accommodations contact Dieter Griffis at above address, or
dgriffis-at-ncsu.edu, or call 919-515-2128

______________________________
Roberto Garcia
rgarcia-at-unity.ncsu.edu
http://www.ncsu.edu/aif
http://www.ncsu.edu/aif/asm

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 19 09:22:20 2004

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Scott,

I was assuming it was not the chip since I have blue fringes toward the
outside of the image and red fringes toward the center. If it was the chip,
I thought the colored fringes would stay on one side. Does that make sense?

Diane

-----Original Message-----
} From: Scott Whittaker [mailto:Whittaker.scott-at-nmnh.si.edu]
Sent: Wednesday, August 18, 2004 4:52 PM
To: Ciaburri, Diane A

Hello,

I'm looking at putting a digital camera (Evolution MP, QIMAGING
MicroPublisher 5.0 RTV) on my Olympus Stereozoom SZH Microscope (with DF
PLAN objectives). In the initial trial, I had some chromatic aberration.
I'm not sure where it's coming from. Does anyone have any experience with
combining this camera and microscope? Any suggestions to reduce chromatic
aberration?

Thanks,

Diane Ciaburri

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 19 09:33:11 2004

Contents Retrieved from Microscopy Listserver Archives
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Jim,

It sounds interesting. Could you give me a few more details about running a
test with a color chart? What kind of color chart, and what would I do with
it?

Thanks!

Diane Ciaburri

-----Original Message-----
} From: Jim Quinn [mailto:jquinn-at-www.matscieng.sunysb.edu]
Sent: Wednesday, August 18, 2004 5:30 PM
To: Ciaburri, Diane A

Dear Listees,

Does anyone have a spare Chiller for our Hitachi 7000, TEM? or do you know
some one who might?

Flow of 4-5 L/Min.
Pressure: 0.5-2 kg/sq. cm
Temp: 10-25 degrees Centigrade

Thanks,

Darryl Krueger
Research Associate
Clemson University

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 19 15:35:04 2004

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I'm dismantling a vintage 1968 Hitachi HU125E TEM that has been our faithful
scope since 1986. Anyone need some parts? Contact me off-list.

Robert
--
Robert Fitton
Teaching Associate/Director of Laboratories
Luther College
Department of Biology
700 College Drive
Decorah, IA 52101

fittonro-at-luther.edu
Voice 563-387-1559
FAX 563-387-1080

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 19 22:07:46 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (rgr-at-georgetown.edu) from
http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Thursday, August 19, 2004 at 08:41:36
---------------------------------------------------------------------------

Email: rgr-at-georgetown.edu
Name: Robert Russell

Organization: Georgetown University

Education: Graduate College

Location: Washington,District of Columbia, USA

Question: I am seeking information about suitable resins/plastics in which to
embed metal stents and similar devices and then
conduct imunohistochemistry staining for biomarkers
in the surrounding tissues - i.e. artery and soft tissue.
This nquiry is about suitable protocols/experience
of plastics or other embedding methods that preserve
the antigens during the polymerization process so
that IHC or immunofluorescence staining can be conducted
for biomarkers. As I understand, it the heat in the
polymerization exothermic reaction may destroy destroy
the antigens. I assume that the plastic requires
pretreatment etching to expose the antigenic epitopes.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 20 09:36:41 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What are the makes of ultramicrotomes currently on the market and what is the consensus about them? I'm aware of RMC (Boeckeler) and Leica. I'm helping a colleague equip her new lab and she needs to submit three bids.

Thank you very much,

Jaclynn Lett
Senior Research Technician, EM Core Facility
Washington University School of Medicine
Department of Otolaryngology
660 South Euclid Ave., Box 8115
St. Louis, MO 63110

email: lettj-at-ent.wustl.edu

Note: NEW PHONE NUMBERS
voice: 314-747-7257
fax: 314-747-7230

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 20 13:33:59 2004

Contents Retrieved from Microscopy Listserver Archives
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I would like to hear from anyone who may be able to help me find and
install a backscatter detector. Our SEM is a JEOL T-220A (built approx.
1987).

Tom Bargar
Core Electron Microscopy Research Facility
Univ. of Nebraska Medical Center
986395 Nebraska Medical Center
Omaha, NE 68198-6395
phone 402-559-7347
e-mail tbargar-at-unmc.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 20 16:42:45 2004

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Robert,
In my lab, we have had very good luck using
butyl-methyl-methacrylate for immuno work. It is polymerized by UV at
4 C, minimizing the heat problem you mentioned, and after sectioning,
the resin can be removed with 10 min in acetone. We also include
dithiothreitol (DTT) in the resin which also seems to keep those
antigens nice and perky. You can find results and information in 1992
Planta 187: 405 - 413. and in 1997 Plant Physiology 115: 101 - 111.
If you are interested, I'll be happy to send you a detailed protocol
and discuss technical stuff. Caveat: I work on plants and have no
idea about the metal stent issue. My answer speaks only for a
generally useful resin for immnocyt.

Hope thise helps,
tobias
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (rgr-at-georgetown.edu) from
} http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
} on Thursday, August 19, 2004 at 08:41:36
} ---------------------------------------------------------------------------
}
} Email: rgr-at-georgetown.edu
} Name: Robert Russell
}
} Organization: Georgetown University
}
} Education: Graduate College
}
} Location: Washington,District of Columbia, USA
}
} Question: I am seeking information about suitable resins/plastics in which to
} embed metal stents and similar devices and then
} conduct imunohistochemistry staining for biomarkers
} in the surrounding tissues - i.e. artery and soft tissue.
} This nquiry is about suitable protocols/experience
} of plastics or other embedding methods that preserve
} the antigens during the polymerization process so
} that IHC or immunofluorescence staining can be conducted
} for biomarkers. As I understand, it the heat in the
} polymerization exothermic reaction may destroy destroy
} the antigens. I assume that the plastic requires
pretreatment etching to expose the antigenic epitopes

From MicroscopyL-request-at-ns.microscopy.com Sat Aug 21 18:25:21 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

I need som advice about BEEM capsules for LR White embedding.

I am developing a work where I want to do both LM and TEM studies.

First I will include material about 8mm (or maybe a little more) diameter
(or major axis measure) in LR White, then I want to look at H&E sections
in order to select an area for appropiate preparation for TEM and
Immunocytochemical analysis.

I am not finding an appropiate BEEM capsule (or equivalent) to include
material without oxygen penetration. I need a capsule which prevent oxygen
penetration and with both flat ends (Top and bottom), not the usual
conical or pyramid end shapes we usually see in standard BEEM capsules
(excellent when the only concern is TEM).

It doesn�t matter if if square or cylindrical what matters is its flat end
for appropiate inclusion of my material.

I�d like to hear suggestion from those of you who might already developed
similar studies.
Does anyone know a supplier(s) where I can get such capsules?

As for LR White polymerization, I only can use an oven so I pretend to
work with 55-60�C. I have no access to ultraviolet.

I'd appreciate both list replies or private ones
(silvia_nit200-micro-at-yahoo.com.br)

Thanks,
Silvia E. Navas

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

From MicroscopyL-request-at-ns.microscopy.com Sat Aug 21 21:51:20 2004

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Hi, Tom

I suppose you've explored the 3rd-party manufacturers, like Oxford Intruments's Tetra,
and GW Electronics.

I don't know your model, but if JEOL ever made one for it, that may be your best bet, at
least for the actual detector, because you know that their one will actually fit without
compromising anything else. The signal-processing electronics is relatively easy.

I fooled around once with bits of solar cell, but it didn't get anywhere close to as good as
the JEOL one for my old JXA-5A.

good luck

rtch

}
}
} I would like to hear from anyone who may be able to help me find and
} install a backscatter detector. Our SEM is a JEOL T-220A (built
} approx. 1987).
}
} Tom Bargar
} Core Electron Microscopy Research Facility
} Univ. of Nebraska Medical Center
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} phone 402-559-7347
} e-mail tbargar-at-unmc.edu
}
}

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand

From MicroscopyL-request-at-ns.microscopy.com Sat Aug 21 23:54:29 2004

Contents Retrieved from Microscopy Listserver Archives
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I used to cut the tips off the BEEM capsules and place an extra cap
on the cut end. This would give me a cylinder with two end caps. The
block would be a little shorter than the standard capsule. Would
that work for you?

Joel

Date sent: Sat, 21 Aug 2004 20:47:52 -0300 (ART)
} From: {silvia_nit2000-micro-at-yahoo.com.br}
Send reply to: silvia_nit2000-micro-at-yahoo.com.br

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (popco-at-toast.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Sunday, August 22, 2004 at 23:36:12
---------------------------------------------------------------------------

Email: popco-at-toast.net
Name: Lloyd McClure

Education: Undergraduate College

Location: White City, OR

Question: Hi,
I just picked up a 2X Bausch microscope of unknown age numbered 312684119.
I have a set of 10X WF eyepieces and would like to know if higher
magnification eyepieces were made for this model and if so, where I
might buy them.
Thanks,
Newbie, Mac McClure

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 00:24:04 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (zerfasp-at-ors.od.nih.gov) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Sunday, August 22, 2004 at 14:06:24
---------------------------------------------------------------------------

Email: zerfasp-at-ors.od.nih.gov
Name: Patricia Zerfas

Organization: NIH

Title-Subject: [Microscopy] [Filtered] MListserver: Lactoferrin gold

Question: Does anyone know of a source for lactoferrin gold? It was
once manufactured by Sigma and was discontinued. I am willing to pay
for the lactoferrin gold and shipping.

Thanks.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 02:48:19 2004

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} Sylvia,
} You can try gelatin capsules of the bigger size,
of course both the
ends are not going to be flat, other option is to try
getting plastic
stoppers for bottles which come in various sizes and
can be used as
mould, unless polymerization has to be done in
airtight capsules.
} regards
} shashi
} CCMB Hyderabad
} INDIA

_______________________________
Do you Yahoo!?
Win 1 of 4,000 free domain names from Yahoo! Enter now.
http://promotions.yahoo.com/goldrush

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 02:47:53 2004

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Re LR White embedding temperature. We can polymerise our
blocks at a tlower temperature - 50 degrees for 48hrs.

Dave

On Sat, 21 Aug 2004 20:47:52 -0300 (ART)
silvia_nit2000-micro-at-yahoo.com.br wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi!
}
} I need som advice about BEEM capsules for LR White embedding.
}
} I am developing a work where I want to do both LM and TEM studies.
}
} First I will include material about 8mm (or maybe a little more) diameter
} (or major axis measure) in LR White, then I want to look at H&E sections
} in order to select an area for appropiate preparation for TEM and
} Immunocytochemical analysis.
}
} I am not finding an appropiate BEEM capsule (or equivalent) to include
} material without oxygen penetration. I need a capsule which prevent oxygen
} penetration and with both flat ends (Top and bottom), not the usual
} conical or pyramid end shapes we usually see in standard BEEM capsules
} (excellent when the only concern is TEM).
}
} It doesn�t matter if if square or cylindrical what matters is its flat end
} for appropiate inclusion of my material.
}
} I�d like to hear suggestion from those of you who might already developed
} similar studies.
} Does anyone know a supplier(s) where I can get such capsules?
}
} As for LR White polymerization, I only can use an oven so I pretend to
} work with 55-60�C. I have no access to ultraviolet.
}
} I'd appreciate both list replies or private ones
} (silvia_nit200-micro-at-yahoo.com.br)
}
} Thanks,
} Silvia E. Navas
}
} __________________________________________________
} Do You Yahoo!?
} Tired of spam? Yahoo! Mail has the best spam protection around
} http://mail.yahoo.com
}
}
}
} This incoming email to UWE has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"

This email has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 08:30:18 2004

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Greetings Everyone,

I would appreciate hearing from anyone who has had experience with field emission SEM both cold and thermal in regards to WDS. Also interested in some of the in lens detectors used for imaging. We are in the process of evaluating FESEM's to add to our laboratory. Please reply off line and if there is enough response I will summarize the information.

Thanks very much,

John Humenansky john.humenansky-at-guidant.com {mailto:john.humenansky-at-guidant.com}
Guidant Corporate Laboratory
4100 Hamline Ave. No.
St. Paul, MN 55117

651-582-6730

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 09:23:58 2004

Contents Retrieved from Microscopy Listserver Archives
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You can buy flat bottom polyethylene of polypropylene capsules through
Pelco (number 133 or 133-P) with an outside diameter of 7.9mm. That may
be big enough for your purpose. I have successfully used them for LR
White.

{} {} {} {} {} {} {} {} {} {}
Kim Rensing PhD
Research Associate
Wood Science, UBC
Vancouver BC, Canada
V6T 1Z4
{} {} {} {} {} {} {} {} {} {}

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 09:41:17 2004

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Hello to all on the listserv-

My boss asked me last week to start researching macrophotography
systems; I managed to turn up a few leads via Google, but it wasn't
much. For those of you who are using such a system, could you please
tell me about it? Which camera, what equipment, software,etc. And, most
importantly, what you like and don't like about it?

Thank you in advance for all your help-
Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Ernest Mario School of Pharmacy
Rutgers University
41 B Gordon Rd
Piscataway, NJ 08854

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 10:19:59 2004

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Dear Sylvia,

We at TAAB Laboratories supply 8mm diameter polythene or polyethylene
capsules with a flat end (instead of the pyramid) and an air tight attached
lid as one of our standard products. Please contact us directly if you think
this may be of interest,

Best regards

Terry Cooper
TAAB Laboratories Equipment Ltd
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England
Tel ++44 (0)118 981 7775 Fax ++44 (0)118 981 7881
e-mail sales-at-taab.co.uk
www.taab.co.uk

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 10:49:46 2004

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In a message dated 8/23/04 12:03:47 PM, kgrobert-at-rci.rutgers.edu writes:

} My boss asked me last week to start researching macrophotography
} systems; I managed to turn up a few leads via Google, but it wasn't
} much. For those of you who are using such a system, could you please
} tell me about it? Which camera, what equipment, software,etc. And, most
} importantly, what you like and don't like about it?

Whoa-- that's way to broad a question for any meaningful answers. What kind
of things are you trying to image - what sizes and particularly what heights
(relevant to the depth-of-field problem)? Are they colored and does reproducing
the color accurately matter? Are they shiny and reflective? Is the purpose to
document and build a file of images, or to make measurements, or to produce
good prints?

There are a lot of camera/software systems that can be used to do a good job
in the hands of someone who has built up some basic photography skills. I
happen to like the Nikon D70 myself, but mostly because I've used Nikons for a
long time and have a bunch of really good lenses. For someone else it might be a
poor choice. Software like Fovea is great for serious processing and
measurement but for some purposes just Photoshop is all you would need (probably it
will be basic to any digital imaging setup). And then there are printers - that
is a field unto itself. A lot of your problems will revolve around proper
lighting, rather than the camera/software choices.

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 12:55:29 2004

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In a message dated 8/23/04 1:44:08 PM, kgrobert-at-rci.rutgers.edu writes:

} Sorry for being so broad-I am a neophyte when it comes to this stuff.
}
} What we are trying to image: We are a university neurotoxicology lab that
} also serves
} as a general core facility for pathology, so we provide services in that
} area-necropsy,
} tissue fixation and processing into paraffin, microtomy, staining (anything
} from H&E to
} IHC) and likewise for TEM and LM. One of the things we would like to do
} is capture
} images of tumors or other pathology in mouse, rat, other animal species,
} etc. organs in
} situ during necropsies as necessary for research purposes. Size, heights
} and color
} will vary; they might be shiny if we wet them down with enough fixative
} to keep them
} moist. I assume that accurate reproduction of color would be necessary.
} The purpose
} here is to document pathology for both research and teaching purposes,
} so measurements
} and good prints would be necessary, and it would be great if we could build
} an archive
} of images.

If you have been doing your photography with film and are just trying to
switch to digital, there are a few things to consider:
1. depending on the camera body you select, you may be able to use your
existing macro lenses, ringlights, and other attachments. That's why I went with
the Nikon D70. It's a fine digital camera, but not the only good one. Take a
look at {http://www.dpreview.com/reviews/specs.asp} for good reviews on most of
the current models. You will certainly want a camera that accepts
interchangeable lenses, not one of the all-in-one models.
2. With that level of camera, you will be able to get the raw output rather
than being limited to a lossy-compressed jpeg image (you will NEVER want to use
jpeg). Photoshop CS will read the raw files and recover more than 8 bits -
sometimes as much as 10 - of data. That is important because with film the
dynamic range (brightness range) that can be handled is much greater than any
digital camera, and you want to capture as much as possible so that the highlights
don't blow out and the shadows plug and hide information (it's worse with
shiny specimens)
3. Check out ReindeerGraphics.com for their package deal on Fovea and
Optipix. The former is great for serious processing, color correction and
measurement. The latter has several tools for macrophotographry and comes with two books
- my "Photoshop for Digital Photographers" and George DeWolfe's guide to
workflow- that will help you to convert from film to digital.
4. If true color is important, you will almost certainly need to get a color
chart (e.g., see gretagmacbeth.com) to include in your scene, if not in every
picture then in every sequence under given lighting. Getting color right is
almost impossible without such a chart.
5. At some point, you will probably want to attend a workshop to pick up some
hands-on training and sharpen your skills. Skip the ones that are
Photoshop-centric - most of those folks are doing creative graphics. But there are still
lots of good choices (a hint - I teach several myself).
6. Before you start, sit down and think very hard about how you are going to
organize the images. There is nothing more frustrating when you have a huge
server or several dozen DVDs full of images and you know you've taken one of a
particular subject, but can't remember how to find it. Searching through the
thumbnails visually is practically worthless. If you use something like the
Photoshop catalog system, you will want to set up lists of keywords beforehand
that can be applied to images in order to make searching more efficient.

John Russ

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 13:02:30 2004

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Workshop Announcement
University of California at Santa Barbara

The Department of Molecular, Cellular, and Developmental Biology and the
Neuroscience Research Institute are sponsoring an advance course on light
microscopy. This 5-day workshop will be offered from September 13 through
September 17, 2004 and will consist of lectures and laboratory exercises
that will run from 9 am to approximately 5 pm each day. The seminar/workshop
will be intensive enabling participants to develop theoretical and hands-on
expertise with light microscopes. Attendees will interact closely with the
instructors while using modern research grade microscopes, cameras, and
computers. The seminars and laboratories will cover basic optical theory and
how it pertains to increasing contrast (signal to noise ratio) in biological
samples. Fundamental techniques such as fluorescence, phase contrast,
Nomarski differential interference contrast, and darkfield imaging will be
taught and attendees will use microscopes equipped with these optical
enhancement accessories. In addition, the theory and practice of electronic
image acquisition (analog and digital) will be discussed and attendees will
work with low-light cameras, digital image processing computers, and
morphometric programs. There are five research grade microscopes, five
electronic imaging cameras, two computer workstations, and one confocal
microscope. With a maximum enrollment of 10 students, there will be ample
opportunity to work with all of the microscopes and cameras. For those so
interested, intensive hands-on instruction and guidance on the confocal
microscope will be provided.

For a fuller description of the workshop, please check the web address
below. Enrollment forms can be completed online and this workshop provides
an opportunity to have a working-vacation in Santa Barbara, California.

http://www.lifesci.ucsb.edu/mcdb/events/events.html

Brian Matsumoto
Adjunct Associate Professor
Dept. MCDB, Neuroscience Research Institute
UCSB
Santa Barbara, CA 93106

voice mail 805-893-8702
FAX 805-893-2005

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 13:33:11 2004

Contents Retrieved from Microscopy Listserver Archives
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I'm not sure what you understand by "macrophotgraphy", particularly
what size object do you want to image full frame.

I've recently used a Nikon Coolpix 8700 (8MB chip, with "macro"
capability), and am not impressed by its performance in close range
(down to approx 2 inches, 5 cm). Depth of field is limited by the max
f-stop of f/8, the built in flash produces a shadow against the
extended lens (even when using the longest macro-allowable focal
length of the zoom), TTL flash exposure compensation does not work at
all (continuous light exposure comp works), and yellow color
overexposes regardless of exposure compensation settings, autofocus
has problems in close range, and there is too little detail on the
LCD screen to allow accurate manual focusing. Although the 8700 is
good for overall scenes (RAW, TIF files), macro capabilities are
disappointing.

I would recommend to use a camera with optical-glass viewfinder (not
a LCD screen) for accurate manual focusing, test the TTL-flash
exposure compensation regardless of manufacturer's claims. Consider
also bellows head lenses; Zeiss Luminars can be obtained on the
second hand market, and some manufacturer's still produce new ones
(Minolta, Olympus, I think). The smaller diameter thread mount makes
them interchangeable between systems, and the narrow diameter of the
lens barrel allows for more freedom in lighting. Depending on
magnification, get a lens with decent f-stop range. Note, that
cranking down the f-stop at higher mag (} 2:1) may cause image
degradation due to diffraction. See Sydney Ray's excellent tome
"Applied Photographic Optics" now in the 3rd edition for details; all
the personal feel, final magnification dependence, aperture blade
numbers.

I use a Contax RTS III with Contax bellows, that allow to place the
focal plane at an angle to the film plane (shift/tilt or PC bellows)
to approx 3.5:1 with a 100 mm Macro (i.e. approx. 8 mm full frame),
or the 50/1.4 reversed a bit higher. It is a film camera, but the
Carl Zeiss optics are wonderful. Some of the older bellows by Nikon
and Minolta also are PC capable, but I am not sure whether they
accept any of the new digital bodies.

RAW files are nice as they are 16 bit/channel, but they are
agonizingly slow to open. The 12 MB RAW/NEF files from the Nikon 8700
takes about 2 minutes to process on a 1 GHz Mac G4. Writing the RAW
files to a 1GB flash card also takes a while, say 20-30 seconds.

Best wishes Daniel

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
***************************************************************************************

Daniel L. Geiger, Ph.D.
Research Associate Santa Barbara Museum of Natural History
Adjunct Assistant Professsor, University of Southern California, Los Angeles

Mailing Address:
Molecular Systematics Lab, Natural History Museum of Los Angeles County
900 Exposition Blvd., Los Angeles, CA 90007, USA
phone: (213) 763 3431 fax: (213) 748 4432
NEW e-mail geiger-at-vetigastropoda.com
NEW www http://www.vetigastropoda.com

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 14:09:57 2004

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I need a mercury arc source for a Zeiss Axiovert 100. I have an old
Olympus power supply for a 100W mercury burner. Does anyone have any
idea whether it would be possible to buy a Zeiss 50 or 100W lamp
house and wire the Olympus supply to drive it? Thanks- Dave
--

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd. U-3125
Storrs, CT 06269-3125
knecht-at-uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 15:54:16 2004

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Adapting a good simple lens such a Zeiss Tessar or Kodak Extar with
as few surfaces and good color correction to a digital camera will
give the best images. Trying to use the photube of stereo microscope
is a bit handier but requires a very good stereo microscope to get
as sharp and high a contrasts images as a old coated cine lens off
ebay. While not as good as the f 6.3 reversed this 45 mm 2.8 Zeiss
Tessar http://www.surplusshed.com/detail.cfm?ID=L2158 sould do a
pretty good job on a digal back at 1 to 1 work. If it was stopped
down to F 8 or f 16. The focal length is a bit long for more
magification.

A Nikon CoolPix with Mark Simmons adapter
http://www.perspectiveimage.com/ and a reversed cine lens work very
well. As does his short tube microscope and good 1x to 4x objective
or Micro Tessars with the eyepice to make the work wiht the lens in
the coolpix. The quality of the objective really shows here. In
http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artjan03/gcshorttube.html
the 12.5 Leitz Photar and Leitz 1x Plan did much better work than
the regualy objectives that I used. The sharpness of the Photar was
really noticable.

A better choice would be a camera with a removable lens or a digital
back for 4 x5 camera. so you would have full movements of the view
camera and a larger chip area.

The lighting is an area that requires a lot of attention as well. I
prefer fiber optics and several of them for control in small areas.
I also use a good deal of electronic flash slaved to the camera.

Gordon
Gordon Couger

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward any links or information you think might be useful to
others.
Microscope Manual at www.science-info.org

----- Original Message -----
} From: "Kathleen Roberts" {kgrobert-at-rci.rutgers.edu}
To: {Microscopy-at-microscopy.com}
Sent: Monday, August 23, 2004 10:13 AM

I've been using a Nikon D1, D100 and D70 and must say that their
performances are quite close to eachother, nevertheless the price. Be aware
that TTL is not always available, eg. not only when working in manual
position you'll have problems, also in the aperture-stand (where you choose
the aperture), you might have overexposed images!

Major thing about the D70 is its price, highly competitive concerning
price/quality compared to others. Negative points: no TIFF available, only
RAW and three JPEG-values (high, medium, low compression) in 3 modes: low,
medium and high resolution. When obtaining for the RAW-file (non-compressed
JPEG at high resolution is enough for high quality printing in the
advertisement-world!), also take in account that the setup of a RAW-file
with the D100 is not the same as with the D70 (i.e. a program that can read
RAW-files from a D100 therefor is not capable of reading the RAW-file from a
D70).

The D1 on the other hand I find outdated, big, heavy, not very user-friendly
and still pricy, where a D70 costs about 1000 euro, a D1 still costs
something like 3000 euro.

My opinion, take in account the Nikon D100. It's a little heavier and
bigger than a D70, allthough not much, offers you the same capabilities, but
also the TIFF-format, which you will be using greatfully! JPEG's are very
nice for regular photography and morphometry, except if intensity and
color-definition is important, here a TIFF will offer you more. RAW is not
always better (in most cases, a TIFF-file has more data in it's pixels than
a RAW-file, allthough this is generally not known nor accepted, check the
digital photography-books and magazines for comprovement!).

A Nikon D100 you'll have for about 1400 euro without objective, a Nikon D70
for 1000 euro (also without objective), but the 400 euro make a difference.
When obtaining an objective (macro!), choose also a Nikon! The difference
in definition of your images is very big, and you will regret the day you
bought an objective from another, cheaper, brand and not one from a known
brand! You'll see the difference in definition, especially when zooming in
to look at the small details (not even talking about aberations and
color-shifts)!
I hope I helped you out a little more concerning Nikon, but it does not mean
that other brands as Canon cannot give you the same! I do not have anything
to do with Nikon, just that I've always been using them in the lab (also in
combination with Zeiss stereomicroscopes (great photo's!) and for personal
use: vacations, macro-photography of mushrooms etc... Good luck!

Sven Terclavers

-----Original Message-----
} From: Daniel Geiger [mailto:geiger-at-vetigastropoda.com]
Sent: Monday, August 23, 2004 20:55
To: kgrobert-at-rci.rutgers.edu; Microscopy-at-microscopy.com

I'm not sure what you understand by "macrophotgraphy", particularly
what size object do you want to image full frame.

I've recently used a Nikon Coolpix 8700 (8MB chip, with "macro"
capability), and am not impressed by its performance in close range
(down to approx 2 inches, 5 cm). Depth of field is limited by the max
f-stop of f/8, the built in flash produces a shadow against the
extended lens (even when using the longest macro-allowable focal
length of the zoom), TTL flash exposure compensation does not work at
all (continuous light exposure comp works), and yellow color
overexposes regardless of exposure compensation settings, autofocus
has problems in close range, and there is too little detail on the
LCD screen to allow accurate manual focusing. Although the 8700 is
good for overall scenes (RAW, TIF files), macro capabilities are
disappointing.

I would recommend to use a camera with optical-glass viewfinder (not
a LCD screen) for accurate manual focusing, test the TTL-flash
exposure compensation regardless of manufacturer's claims. Consider
also bellows head lenses; Zeiss Luminars can be obtained on the
second hand market, and some manufacturer's still produce new ones
(Minolta, Olympus, I think). The smaller diameter thread mount makes
them interchangeable between systems, and the narrow diameter of the
lens barrel allows for more freedom in lighting. Depending on
magnification, get a lens with decent f-stop range. Note, that
cranking down the f-stop at higher mag (} 2:1) may cause image
degradation due to diffraction. See Sydney Ray's excellent tome
"Applied Photographic Optics" now in the 3rd edition for details; all
the personal feel, final magnification dependence, aperture blade
numbers.

I use a Contax RTS III with Contax bellows, that allow to place the
focal plane at an angle to the film plane (shift/tilt or PC bellows)
to approx 3.5:1 with a 100 mm Macro (i.e. approx. 8 mm full frame),
or the 50/1.4 reversed a bit higher. It is a film camera, but the
Carl Zeiss optics are wonderful. Some of the older bellows by Nikon
and Minolta also are PC capable, but I am not sure whether they
accept any of the new digital bodies.

RAW files are nice as they are 16 bit/channel, but they are
agonizingly slow to open. The 12 MB RAW/NEF files from the Nikon 8700
takes about 2 minutes to process on a 1 GHz Mac G4. Writing the RAW
files to a 1GB flash card also takes a while, say 20-30 seconds.

Best wishes Daniel

} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
****************************************************************************
***********

Daniel L. Geiger, Ph.D.
Research Associate Santa Barbara Museum of Natural History
Adjunct Assistant Professsor, University of Southern California, Los Angeles

Mailing Address:
Molecular Systematics Lab, Natural History Museum of Los Angeles County
900 Exposition Blvd., Los Angeles, CA 90007, USA
phone: (213) 763 3431 fax: (213) 748 4432
NEW e-mail geiger-at-vetigastropoda.com
NEW www http://www.vetigastropoda.com

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 16:06:43 2004

Contents Retrieved from Microscopy Listserver Archives
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As far as I understand, BEEM capsules (at least original ones) are not good
for LR White. Don't ask me why, it's a mystery! 8 mm also looks like too
big to fit a standard ultratome holder. I had similar problem previously
doing EM on 0.2 mm mouse brain slices. I solved it by flat embedding of
the brain slices in LR White (between two sheets of polypropylene) and then
gluing the sample to the standard post with epoxy. Gluing appeared to be a
problem - it seems to me that LR White at high humidity adsorbs water and
do not stick well to the posts... Otherwise, it was working quite well.
Additional reason to do flat-embedding is because such large (8 mm) samples
tends to be curved, so I actually straighten them between two
polypropylene sheets with some force. You need to understand that plastic
will not polymerize at the edges, so sheets should be much bigger than your
sample.
I hope it may help. Sergey

At 08:47 PM 8/21/2004 -0300, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 16:13:12 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kathleen,

I agree with most of what John says in his posting. A couple of things I
would like to add:

1) You may want to check out if a "copystand" or some other device that lets
you set up more consistent illumination and imaging conditions is the right
thing to use. this would also allow you to apply correct calibrations to
your images. Call me if you have any questions.

2) I would also consider a microscope camera with live imaging on the
computer screen. That way it is a lot easier to adjust lighting or exposure
to avoid reflections and/or dark areas.

3) Photoshop is certainly a capable application. However, you may want to
investigate other software that is geared more towards microscopy. You may
want to use the same software for microscopes as well, and then other
software might be better suited. You can start by looking at our website,
but there are other vendors as well.

4) I could not agree more with John regarding cataloging the images. Again,
I think you can do better than Photoshop, especially when it comes to
automatically reading parameters from microscopes, etc.

5) From your address I see that your work is related to Pharma. I don't know
if FDA rules apply to your work, but if so, make sure that the software you
use is Rule 11 compliant. There is a good chance that you don't need this,
but I'd make sure. Again, you can check our web site for more information.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: DrJohnRuss-at-aol.com [mailto:DrJohnRuss-at-aol.com]
Sent: Monday, August 23, 2004 12:17
To: kgrobert-at-rci.rutgers.edu; microscopy-at-microscopy.com

In a message dated 8/23/04 1:44:08 PM, kgrobert-at-rci.rutgers.edu writes:

} Sorry for being so broad-I am a neophyte when it comes to this stuff.
}
} What we are trying to image: We are a university neurotoxicology lab that
} also serves
} as a general core facility for pathology, so we provide services in that
} area-necropsy,
} tissue fixation and processing into paraffin, microtomy, staining (anything
} from H&E to
} IHC) and likewise for TEM and LM. One of the things we would like to do
} is capture
} images of tumors or other pathology in mouse, rat, other animal species,
} etc. organs in
} situ during necropsies as necessary for research purposes. Size, heights
} and color
} will vary; they might be shiny if we wet them down with enough fixative
} to keep them
} moist. I assume that accurate reproduction of color would be necessary.
} The purpose
} here is to document pathology for both research and teaching purposes,
} so measurements
} and good prints would be necessary, and it would be great if we could build
} an archive
} of images.

If you have been doing your photography with film and are just trying to
switch to digital, there are a few things to consider:
1. depending on the camera body you select, you may be able to use your
existing macro lenses, ringlights, and other attachments. That's why I went
with
the Nikon D70. It's a fine digital camera, but not the only good one. Take a

look at {http://www.dpreview.com/reviews/specs.asp} for good reviews on most
of
the current models. You will certainly want a camera that accepts
interchangeable lenses, not one of the all-in-one models.
2. With that level of camera, you will be able to get the raw output rather
than being limited to a lossy-compressed jpeg image (you will NEVER want to
use
jpeg). Photoshop CS will read the raw files and recover more than 8 bits -
sometimes as much as 10 - of data. That is important because with film the
dynamic range (brightness range) that can be handled is much greater than
any
digital camera, and you want to capture as much as possible so that the
highlights
don't blow out and the shadows plug and hide information (it's worse with
shiny specimens)
3. Check out ReindeerGraphics.com for their package deal on Fovea and
Optipix. The former is great for serious processing, color correction and
measurement. The latter has several tools for macrophotographry and comes
with two books
- my "Photoshop for Digital Photographers" and George DeWolfe's guide to
workflow- that will help you to convert from film to digital.
4. If true color is important, you will almost certainly need to get a color

chart (e.g., see gretagmacbeth.com) to include in your scene, if not in
every
picture then in every sequence under given lighting. Getting color right is
almost impossible without such a chart.
5. At some point, you will probably want to attend a workshop to pick up
some
hands-on training and sharpen your skills. Skip the ones that are
Photoshop-centric - most of those folks are doing creative graphics. But
there are still
lots of good choices (a hint - I teach several myself).
6. Before you start, sit down and think very hard about how you are going to

organize the images. There is nothing more frustrating when you have a huge
server or several dozen DVDs full of images and you know you've taken one of
a
particular subject, but can't remember how to find it. Searching through the

thumbnails visually is practically worthless. If you use something like the
Photoshop catalog system, you will want to set up lists of keywords
beforehand
that can be applied to images in order to make searching more efficient.

John Russ

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 17:08:49 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You can buy flat-bottomed capsules, most microscopy suppliers should have
them. For example, EMS
(http://www.emsdiasum.com/microscopy/products/catalog.aspx ) has them under
Specimen preparation.... Vials and sample storage. I used to use some from
TAAB a few years ago. Even if they aren't in the catalogue, many suppliers
can find them for you.

good luck,
Rosemary

No commercial affiliation, etc, etc

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5000
Canberra, ACT 2601
Australia

} From: shashi singh {shashis_99-at-yahoo.com}
} Date: Mon, 23 Aug 2004 01:09:51 -0700 (PDT)
} To: microscopy-at-msa.microscopy.com
} Cc: silvia_nit200-micro-at-yahoo.com.br
}
}
}
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} } Sylvia,
} } You can try gelatin capsules of the bigger size,
} of course both the
} ends are not going to be flat, other option is to try
} getting plastic
} stoppers for bottles which come in various sizes and
} can be used as
} mould, unless polymerization has to be done in
} airtight capsules.
} } regards
} } shashi
} } CCMB Hyderabad
} } INDIA
}
}
}
}
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} Win 1 of 4,000 free domain names from Yahoo! Enter now.
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From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 22:35:46 2004

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Dear fellow microscopists,

I am looking for comments regarding a general protocol for DAB staining
for correlative light and TEM. I found a procedure of Essner (1973) that
uses 3% glut fixation, incubation in DAB for 2 hours, followed by 1%
hydrogen peroxide, subsequent washing steps, and incubation in buffered
KFeCN (3 mM) to eliminate non-specific adsorption of DAB. Samples are
further fixed with OsO4, dehydrated and embedded in epoxy resin.

I would like to hear tips from people who have success with this or
other DAB protocol for TEM. We are trying to visualize heme-containing
cytochromes in bacteria.

Many thanks in advance,
Alice.

Alice Dohnalkova
Research Scientist
Environmental Microbiology
Pacific Northwest National Laboratory
Richland, WA 99352
(509) 372-0692 office
(509) 376-3654 TEM lab

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 23 23:23:17 2004

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Hi, Kathleen,

I'd suggest you take a look at Microptics. They have a great system ...
fully integrated and very functional and cost-effective.

You should be able to find them on the web. If you can't, get in touch
with me offline and I'll find their info for you.

Caveat: MME has no financial interest in this company.

Good hunting

Barbara Foster
Microscopy/Microscopy Education

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
At 10:13 AM 8/23/2004, Kathleen Roberts wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 24 00:15:18 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (wall1-at-llnl.gov) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Monday, August 23, 2004 at 11:08:27
---------------------------------------------------------------------------

Email: wall1-at-llnl.gov
Name: Mark A. Wall

Organization: LLNL

Title-Subject: [Microscopy] [Filtered] MListserver: CBED software

Question: I would like information on software (free or commercial)
for the simulation/comparison of HOLZ lines in CBED patterns. For
lattice parameter and strain measurements.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 24 03:54:32 2004

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: }
: } Hello to all on the listserv-
: }
: } My boss asked me last week to start researching macrophotography
systems;
: } I managed to turn up a few leads via Google, but it wasn't much.
For
: } those of you who are using such a system, could you please tell
me about
: } it? Which camera, what equipment, software,etc. And, most
importantly,
: } what you like and don't like about it?
: }
: } Thank you in advance for all your help-
: } Kathleen Roberts
: } Principal Lab Technician
: } Neurotoxicology Labs
: } Dept of Pharmacology & Toxicology
: } Ernest Mario School of Pharmacy
: } Rutgers University
: } 41 B Gordon Rd
: } Piscataway, NJ 08854
: }

Evaluate what you already have first. Particularly in skills.

If you set up a comprehensive faculty the camera is a small part of
the expense. A really good camera to build a versatile systems
hasn't really been made yet. The closest are the backs for 4X5
camera to use in existing set ups using lenses and lighting you
already have. A better choice would be a system that used a digital
camera that could use your existing 35 mm macro equipment. Most
reports are not very encouraging.

Jan Hinsch wrote about using a Digital SLR on a microscope for
McCrone's www.ModernMicroscopy.com
http://www.modernmicroscopy.com/main.asp?article=33 and was
satisfied. Ted Clarke discuses some of the issues of resolution of
digital image in the same online journal.
http://www.modernmicroscopy.com/main.asp?article=31

With a digital SLR the first problem all your lenses are twice the
effective size due to the smaller image area of the CCD. Other
problems include the inability to take long exposures, many cameras
can't lock the mirror up, problems with preview on some cameras and
mirror lock up. Mostly the cameras haven't evolved to the point that
the features need for macro work are available on enough cameras.
The lack of long exposure times is a problem with noise form heat
that can be solved by cooling the sensor or with software by adding
images.

Before you spend a lot of time on the project find out what they
mean by macro photography. If you are replacing a 4X5 with a full
set of Zeiss micro Tessars on a Leitz Astrophot stand with all the
nice lighting accessories that go with it and an operator that knows
how to use them a 4x5 digital insert for the camera will probably be
the way to go.

If you are starting from scratch and need to take product shots of
pills a good consumer camera may do the job with a careful selection
of lighting and backgrounds. I expect you application falls
somewhere in between. But getting the answer before you understand
the problem will most likely be time wasted.

Good luck
Gordon
Gordon Couger gcc-at-couger.com

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward any links or information you think might be useful to
others.
Microscope Manual at www.science-info.org

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 24 04:39:43 2004

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Chair in Materials Science, Institute of Materials Science & Technology
(IMT), Friedrich-Schiller University (FSU) Jena, Germany
�
Postdoctoral research scientist or PhD student position (government
pay scale BAT-O IIa for initially 24 months, full time or part time,
depending on qualification, subject to approval of funds) to be filled
by 1. October 2004 in the research area:
�
Phase Boundaries of Proteins and Biomaterials
�
Aim of the project is an enhanced understanding of the interaction of
biological molecules or cells and different biomaterials.
The interest is in understanding the correlation between materials
structure and properties and the process of biological reactions, such
as blood coagulation or complement activation.
The ideal candidate for this position has sound experience in atomic
force microscopy (AFM) and is interested to work with TEM, CLSM, XPS
applied to biomaterials.
A background in one or more of the following areas is suitable:
physics, biophysics biomaterials, materials science, chemistry/polymer
chemistry, biology, engineering or related disciplines.
�
There are opportunities to co-operate with the members of the Start-up
Centre for Young Entrepreneurs at the department.
The group co-operates closely with other groups at the FSU, nationally
and internationally leading universities of the Anglo-American
countries as well as industrial partners.
�
Candidates for postdoctoral positions are requested to submit a
one-page research proposal.
Submit your full application, quoting ref. 34/04 including CV, photo,
research experience and qualifications and have two academic references
forwarded to
�
Prof. Dr. Klaus D. Jandt
(k.jandt-at-uni-jena.de)
Institute of Materials Science and Technology (IMT)
Friedrich-Schiller University Jena
L�bdergraben 32,
D-07743 Jena
Germany.
�
Closing date is 15. September 2004.
�
This is an unofficial, shortened translation of the German original
advertisement text which can be found at
http://www2.uni-jena.de/matwi/. This translation is not legally
binding.
http://www.uni-jena.de/matwi/

-----------------------------------------------------------------
Prof. Dr. Klaus D. Jandt
Chair in Materials Science
Director of Institute of Materials Science and Technology (IMT)
Friedrich-Schiller-University Jena
L�bdergraben 32
D-07743 Jena
Germany
Phone: ++ 49 3641 947730
Fax: ++ 49 3641 947732
Internet: K.Jandt-at-uni-jena.de

Visit us under http://www.uni-jena.de/matwi/

"Making Materials Science Work 4 U"

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 24 08:14:24 2004

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Mark,

check out our ASAC (Automatic Strain Calculation by CBED,
http://www.soft-imaging.com/rd/english/410.htm) product on our web site. It
is currently implemented for Si at various zone axes.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: wall1-at-llnl.gov [mailto:wall1-at-llnl.gov]
Sent: Monday, August 23, 2004 23:38
To: microscopy-at-ns.microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (wall1-at-llnl.gov) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Monday, August 23, 2004 at 11:08:27
---------------------------------------------------------------------------

Email: wall1-at-llnl.gov
Name: Mark A. Wall

Organization: LLNL

Title-Subject: [Microscopy] [Filtered] MListserver: CBED software

Question: I would like information on software (free or commercial)
for the simulation/comparison of HOLZ lines in CBED patterns. For
lattice parameter and strain measurements.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 24 13:32:24 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We recently purchased a used Hitachi H7000 (without manuals,
schematics, etc.) and have a question related to magnification.

Does the H7000 generate a magnification marker bar on the negative?
If so, how is this activated from the menu?

Thanks very much.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 24 16:32:05 2004

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Dear All,

I have need to automate the Z axis on our JEOL JSM-6400 stage (same as
840). At one time JEOL made an automated Z-axis system that they
installed on the JXA-840 systems. Follow this link to see a few images
of this assembly.

http://www.mse.mtu.edu/~opmills/zaxis.htm

Do you have this part in a drawer or cabinet somewhere? I am seeking
to buy or have this part donated.

Please reply to me if you have this part or know one's whereabouts.

Thanks!

Owen

Owen P. Mills
Electron Optics Engineer
Applied Chemical & Morphological Analysis Laboratory
Materials Science & Engineering
Michigan Technological University
Rm 512 M&M Bldg.
Houghton, MI 49931
PH 906-369-1875
FAX 906-487-2934
mailto:opmills-at-mtu.edu
http://www.mm.mtu.edu/~opmills

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 24 16:53:13 2004

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Does anyone have experience with the EXFO X-cite 120 light source for
fluorescence microscopy? THanks-Dave
--

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd. U-3125
Storrs, CT 06269-3125
knecht-at-uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 25 02:13:51 2004

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Greetings All,

After many years of faithful service an old Sony CCD has passed on. It was
used on a Zeiss Stemi SV-11 and images were captured on a MAC G4 running OS
8.6, via Photoshop 5.5 using Import/Export plugins.

Can anyone recommend a camera that would:
1. Fit on a Zeiss Stemi SV-11
2. Interface with a MAC (preferably via Firewire)
3. Interface with Photoshop
4. Include micron markers with the captured image

I realise that I will probably have to upgrade the OS and add some RAM.

Any suggestions would be greatly appreciated.

Regards
George

George Theodossiou
Physicist / Electron Microscopist

AMCOR Research and Technology
Ph: +61 3 9490 6135
Fax: +61 3 9499 4295
Mobile: 0409 568 840
email: George.Theodossiou-at-amcor.com.au

************************************************************************
CAUTION - This message may contain privileged and confidential
information intended only for the use of the addressee named above.
If you are not the intended recipient of this message you are hereby
notified that any use, dissemination, distribution or reproduction of
this message is prohibited. If you have received this message in error
please notify AMCOR immediately.
Any views expressed in this message are those of the individual sender
and may not necessarily reflect the views of AMCOR.
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From MicroscopyL-request-at-ns.microscopy.com Wed Aug 25 03:22:59 2004

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John

I'm afraid that the basic Hitachi H7000 doesn't generate a micron mark, although the STEM and SEM system does. I just checked a couple of old brochures and the micron mark capability seems to have come in with the H 7100.

Let me know if you need any help with the manuals or basic schematics for the H7000.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
} From: "John J. Bozzola" {bozzola-at-siu.edu}

Our data collection contractor in Virginia has been instructed to close the
MT Salary and Equipment Usage Survey on September 1st.

This survey was mailed to nearly 17,000 MT subscribers in June and
handed-out in hard copy at the M&M meeting in Savannah.

If you have already sent your completed survey form to Virginia, thank you.
If you have been putting it off, please mail it in TODAY!

If you wish to remain anonymous, just fill in your home state so we can
report salary vs. state data.

The drawing for the hand-held TV prize will take place in the first week in
September. Naturally, we cannot award the prize to anonymous submissions.

If you live in the USA and Canada and want to participate, send me an email
and I'll fax you the survey plus the fax number of the data collection
contractor.

Ron Anderson, Editor
Microscopy Today

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 25 16:20:28 2004

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Greetings David,
Toludine blue and sodium borohydride are also commonly used quenching
agents where broadly emmitting background fluorescence is concerned.
Trypan blue is an acid dye and will bind positively charged moeties, and
toluidine blue is a basic stain known to bind negatively charged
substrates such as chromatin. The quenching idea simply involves
getting some chemical moiety to absorb the frequency being emmitted, a
solution of green absorbing fluorochrome such as rhodamine may do the
same thing without absorbing your illumination source as much (I'm
assuming these are adherent cells). I'm not entirely sure what sodium
borohydride does, but it is a popular method for quenching
autofluorescence in the presence of GFP.
One approach which might prove to be a more specific (and expensive)
system for quenching would involve the use of anti-FITC antibodies (I
believe Sigma carries them), although I'm not positive that the bound
anti-body will quench the fluorochome. Anti-fluorochrome antibodies
will quench some target fluorochromes. One could also label the FITC
targets with a different color fluorochrome through the use of
antibodies to FITC. The alternate fluorochrome or a gold conjugate may
be in close enough proximity for non-radiative transfer and subsequent
quenching to occur.
Regards,
Karl

David Knecht wrote:

}
}
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}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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}
}
} Trypan quenching of fluroescence has been used for years to decrease
} fluorescence of particles and dextran. In our case, we are trying to
} use it to quench the fluorescence of surface labeled particles to
} distinguish internalized particles (phagocytosis) from external
} particles. We have not gotten significant quenching, and as I read
} the literature, I am unclear as to how this is supposed to work. In
} the original Hed paper that most people reference, they use FITC
} labeled particles and quench by putting the cells in a trypan solution
} in a pH 4.5 buffer. They describe this as a FRET type quenching to a
} non-radiative acceptor. Trypan does emit, but lets leave this aside
} for now. Their explanation makes little sense to me since with the
} known pH dependence of FITC, it seems to me they are doing primarily
} pH based quenching as opposed to FRET based quenching. If so, then
} are they simply looking at the difference in FITC fluorescence between
} the phagosomal pH (5.5) and the external pH (4.5)?
} In other papers, rhodamine labelled particles are used, and trypan
} quenching is also supposed to work, however in our case, we can only
} see about 50% quenching. Given how opaque the solution is, I could
} easily see that being a case of simply decreasing both excitation and
} emission due to opacity of the solution. In some cases (yeast
} particles) I have been told that the trypan actually binds to the
} particles so that you can wash it out and the particles are still
} blue, and in that case, I would imagine very different quenching
} properties, since the trypan is no longer freely diffusing in
} solution. I have yet to find literature that clearly investigates the
} quenching phenomenon with different fluors under solution vs. bound
} conditions. Can anyone help? Thanks- Dave

--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 25 17:55:46 2004

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Dear colleagues
I am looking for simple sign-in calendar software (preferably freeware), so
users could sign-in for the instruments on the WEB. I expect this software
should work on my server (windows based) and provide easy way to sign-in,
sign-out for users for the different instruments. I do remember, this
issue was discussed on the server a few years ago but I want to see what
people currently used. I was trying Google search and it was not so
productive: most links were addressed to the calendars hosted on the
Internet (like free E. mail accounts) or calendars for the business
(intranet). I would really appreciate your input. Have a great day
(night?). Sergey.

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Aug 25 19:55:54 2004

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Dear listers,
Does anyone have access to a set of schematics (11" x 17") for the Amray
1000? This is not the 1000B (and 1600 series) with the microprocessor-based
mag control, but the original 1000 with analog controls
We can copy and return originals or pay copy and shipping costs. Please
contact Earl Weltmer at: earlw-at-sbcglobal.net

Or me, below.

Thanks,

Ken Converse

owner�
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

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From MicroscopyL-request-at-ns.microscopy.com Wed Aug 25 23:08:29 2004

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Good to hear someone is still running a 1000, as we have not set ours up
yet, it and all the documentation is boxed up. I fear it may be behind a few
thousand pounds of boxes in the warehouse, but I can check on it. we ended
up with the unit from Honeywell computer plant here in phoenix.

It would be good to know who else out there is running one of these units. I
suspect we are a small group. Just have Amray in the title of the message
and it will catch my eye.

Thanks Ed Sharpe, Archivist for SMECC - - See the Museum's Web Site at
www.smecc.org

Coury House / SMECC
5802 W. Palmaire Ave. Phone 623-435-1522
Glendale Az 85301 USA
----- Original Message -----
} From: "Ken Converse" {kenconverse-at-qualityimages.biz}
To: "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com}
Sent: Wednesday, August 25, 2004 6:18 PM

Hi,

I just would like to inform you that if you want to change
with Nikon D1, D100 or D70 from macroscopy to microscopy we
have camera adapters for SLR cameras and many other cameras
which fit for your microscope.

If you are interested let me know, I send you a camera list.

mfg / regards

Anneliese Schmaus
Product Manager

klughammer gmbh
Strassbach 9
85229 Markt Indersdorf
Germany
Tel. +49 08136 6011
Fax +49 08136 7098
info-at-klughammer.de
www.klughammer.de

ST} ------------------------------------------------------------------------------
ST} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
ST} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
ST} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
ST} -------------------------------------------------------------------------------

ST} I've been using a Nikon D1, D100 and D70 and must say that their
ST} performances are quite close to eachother, nevertheless the price. Be aware
ST} that TTL is not always available, eg. not only when working in manual
ST} position you'll have problems, also in the aperture-stand (where you choose
ST} the aperture), you might have overexposed images!

ST} Major thing about the D70 is its price, highly competitive concerning
ST} price/quality compared to others. Negative points: no TIFF available, only
ST} RAW and three JPEG-values (high, medium, low compression) in 3 modes: low,
ST} medium and high resolution. When obtaining for the RAW-file (non-compressed
ST} JPEG at high resolution is enough for high quality printing in the
ST} advertisement-world!), also take in account that the setup of a RAW-file
ST} with the D100 is not the same as with the D70 (i.e. a program that can read
ST} RAW-files from a D100 therefor is not capable of reading the RAW-file from a
ST} D70).

ST} The D1 on the other hand I find outdated, big, heavy, not very user-friendly
ST} and still pricy, where a D70 costs about 1000 euro, a D1 still costs
ST} something like 3000 euro.

ST} My opinion, take in account the Nikon D100. It's a little heavier and
ST} bigger than a D70, allthough not much, offers you the same capabilities, but
ST} also the TIFF-format, which you will be using greatfully! JPEG's are very
ST} nice for regular photography and morphometry, except if intensity and
ST} color-definition is important, here a TIFF will offer you more. RAW is not
ST} always better (in most cases, a TIFF-file has more data in it's pixels than
ST} a RAW-file, allthough this is generally not known nor accepted, check the
ST} digital photography-books and magazines for comprovement!).

ST} A Nikon D100 you'll have for about 1400 euro without objective, a Nikon D70
ST} for 1000 euro (also without objective), but the 400 euro make a difference.
ST} When obtaining an objective (macro!), choose also a Nikon! The difference
ST} in definition of your images is very big, and you will regret the day you
ST} bought an objective from another, cheaper, brand and not one from a known
ST} brand! You'll see the difference in definition, especially when zooming in
ST} to look at the small details (not even talking about aberations and
ST} color-shifts)!
ST} I hope I helped you out a little more concerning Nikon, but it does not mean
ST} that other brands as Canon cannot give you the same! I do not have anything
ST} to do with Nikon, just that I've always been using them in the lab (also in
ST} combination with Zeiss stereomicroscopes (great photo's!) and for personal
ST} use: vacations, macro-photography of mushrooms etc... Good luck!

ST} Sven Terclavers

ST} -----Original Message-----
} } From: Daniel Geiger [mailto:geiger-at-vetigastropoda.com]
ST} Sent: Monday, August 23, 2004 20:55
ST} To: kgrobert-at-rci.rutgers.edu; Microscopy-at-microscopy.com
ST} Subject: [Microscopy] Re: Digital Macrophotography

ST} ----------------------------------------------------------------------------
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ST} ---

ST} I'm not sure what you understand by "macrophotgraphy", particularly
ST} what size object do you want to image full frame.

ST} I've recently used a Nikon Coolpix 8700 (8MB chip, with "macro"
ST} capability), and am not impressed by its performance in close range
ST} (down to approx 2 inches, 5 cm). Depth of field is limited by the max
ST} f-stop of f/8, the built in flash produces a shadow against the
ST} extended lens (even when using the longest macro-allowable focal
ST} length of the zoom), TTL flash exposure compensation does not work at
ST} all (continuous light exposure comp works), and yellow color
ST} overexposes regardless of exposure compensation settings, autofocus
ST} has problems in close range, and there is too little detail on the
ST} LCD screen to allow accurate manual focusing. Although the 8700 is
ST} good for overall scenes (RAW, TIF files), macro capabilities are
ST} disappointing.

ST} I would recommend to use a camera with optical-glass viewfinder (not
ST} a LCD screen) for accurate manual focusing, test the TTL-flash
ST} exposure compensation regardless of manufacturer's claims. Consider
ST} also bellows head lenses; Zeiss Luminars can be obtained on the
ST} second hand market, and some manufacturer's still produce new ones
ST} (Minolta, Olympus, I think). The smaller diameter thread mount makes
ST} them interchangeable between systems, and the narrow diameter of the
ST} lens barrel allows for more freedom in lighting. Depending on
ST} magnification, get a lens with decent f-stop range. Note, that
ST} cranking down the f-stop at higher mag (} 2:1) may cause image
ST} degradation due to diffraction. See Sydney Ray's excellent tome
ST} "Applied Photographic Optics" now in the 3rd edition for details; all
ST} the personal feel, final magnification dependence, aperture blade
ST} numbers.

ST} I use a Contax RTS III with Contax bellows, that allow to place the
ST} focal plane at an angle to the film plane (shift/tilt or PC bellows)
ST} to approx 3.5:1 with a 100 mm Macro (i.e. approx. 8 mm full frame),
ST} or the 50/1.4 reversed a bit higher. It is a film camera, but the
ST} Carl Zeiss optics are wonderful. Some of the older bellows by Nikon
ST} and Minolta also are PC capable, but I am not sure whether they
ST} accept any of the new digital bodies.

ST} RAW files are nice as they are 16 bit/channel, but they are
ST} agonizingly slow to open. The 12 MB RAW/NEF files from the Nikon 8700
ST} takes about 2 minutes to process on a 1 GHz Mac G4. Writing the RAW
ST} files to a 1GB flash card also takes a while, say 20-30 seconds.

ST} Best wishes Daniel

} } ---------------------------------------------------------------------------
ST} ---
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

ST} --
ST} ****************************************************************************
ST} ***********

ST} Daniel L. Geiger, Ph.D.
ST} Research Associate Santa Barbara Museum of Natural History
ST} Adjunct Assistant Professsor, University of Southern California, Los Angeles

ST} Mailing Address:
ST} Molecular Systematics Lab, Natural History Museum of Los Angeles County
ST} 900 Exposition Blvd., Los Angeles, CA 90007, USA
ST} phone: (213) 763 3431 fax: (213) 748 4432
ST} NEW e-mail geiger-at-vetigastropoda.com
ST} NEW www http://www.vetigastropoda.com

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 26 06:55:25 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear vacationing listers,
Last night I posted a message. This morning I received 17 e-mails: 4 were
submissions to the list server, 3 were other e-mails and the following 10
were auto replies from the Listserver.

Mei Lie Wong [wong-at-msg.ucsf.edu]
moritz-andreas.meyer-at-amd.com
Oparowski, Joseph [Joseph_Oparowski-at-bose.com]
scosgrove [scosgrove-at-diaginc.com]
Rice, Trisha [TRice-at-FEICO.com]
Dunlap, Jonathan C. [Jonathan.Dunlap-at-sylvania.com]
Stewart Bean [bean-at-smt.zeiss.com]
rhumphre-at-ucalgary.ca
Postmaster-at-mxg.usuhs.mil
Mike Ingram [MIngram-at-rohmhaas.com]

Please read the rules of use. Please review Nestor�s many suggestions in
the archives. Please don�t just add to all the spam we all get.

Thank you for your consideration,

Ken Converse

owner�
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

___________________________________________________________
$0 Web Hosting with up to 120MB web space, 1000 MB Transfer
10 Personalized POP and Web E-mail Accounts, and much more.
Signup at www.doteasy.com

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 26 08:50:41 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Sergey,

I was looking for sign-up calendar software recently myself as we were
trying to set up on-line instrument reservation and most of what I found was
web calendar hosting service such as http://www.calendars.net/. I know that
some laboratories are using it and quite happy.

Our lab is using Calcium software from http://www.brownbearsoftware.com/
that we run from our own server. It is not free unfortunately, but except
for a few minor things we are quite happy with it. Contact me if you need
more feedback on Calcium.

Evgenia

====================================
Evgenia Pekarskaya
Keck Electron Microscopy Laboratory
University of Massachusetts, Amherst
Tel. 413 545 2261
E-fax 325 202 7338
pekarskaya-at-mail.pse.umass.edu
====================================

-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, August 25, 2004 7:19 PM
To: Microscopy-at-microscopy.com

Dear colleagues
I am looking for simple sign-in calendar software (preferably freeware), so
users could sign-in for the instruments on the WEB. I expect this software
should work on my server (windows based) and provide easy way to sign-in,
sign-out for users for the different instruments. I do remember, this
issue was discussed on the server a few years ago but I want to see what
people currently used. I was trying Google search and it was not so
productive: most links were addressed to the calendars hosted on the
Internet (like free E. mail accounts) or calendars for the business
(intranet). I would really appreciate your input. Have a great day
(night?). Sergey.

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 26 09:24:44 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

List,

As a result of a new instrument purchase, we are going to offer for sell our Scintag PadV X-ray Powder Diffraction System.

The system is configured with a vertical 2-Theta:Theta X-ray goniometer, high intensity Cu X-ray tube (replaced about 3 years ago), and a Kevex psi peltier cooled silicon detector. Automation and data collection is accomplished through a Compaq computer running Windows NT and the latest version of Scintag's DMSNT software including stress analysis.

Some spare parts will also be available with the system.

Contact me directly if you have an interest in this instrument.

Regards

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-mail.smu.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 26 11:11:32 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ryan.davis-at-hydro.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Thursday, August 26, 2004 at 09:30:09
---------------------------------------------------------------------------

Email: ryan.davis-at-hydro.com
Name: Ryan Davis

Title-Subject: [Microscopy] [Filtered] MListserver: Unstable emission current

Question: Over the past couple of weeks I watched the emission
current on our Hitachi 3500N jump around like a dancing lady. I have
spoken with the service engineer, and he seemingly thinks dust might
be in the gun chamber. So, I wiped the gun chamber down using
Kimwipes and ethanol, and blew out the gun chamber out to remove any
fibers or debris left behind after cleaning. After cleaning I am
still having this problem. So, does anyone have any tricks for
removing dust?

} A list of things I have checked or replaced:

} Checked for whiskers on the filament. Saw none.
} Replaced filament. Emission current still unstable.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 26 13:24:30 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

Do you know who is going to give up his ESEM E3?
Is there an ESEM E3 near Washington Thank you for
your help.

Tim
}
}
}
}
}
}
} __________________________________
} Do you Yahoo!?
} New and Improved Yahoo! Mail - 100MB free storage!
} http://promotions.yahoo.com/new_mail

} ATTACHMENT part 2 message/rfc822
} From: "Ken Converse" {kenconverse-at-qualityimages.biz}
} To: "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com}
} Subject: [Microscopy] Amray 1000 schematics
} Date: Wed, 25 Aug 2004 21:18:03 -0400
}
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Dear listers,
} Does anyone have access to a set of schematics (11"
} x 17") for the Amray
} 1000? This is not the 1000B (and 1600 series) with
} the microprocessor-based
} mag control, but the original 1000 with analog
} controls
} We can copy and return originals or pay copy and
} shipping costs. Please
} contact Earl Weltmer at: earlw-at-sbcglobal.net
}
} Or me, below.
}
} Thanks,
}
} Ken Converse
}
} owner�
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 16 Creek Rd.
} Delta, PA 17314
} 717-456-5491
} Fax 717-456-7996
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
}
}
}
}
___________________________________________________________
} $0 Web Hosting with up to 120MB web space, 1000 MB
} Transfer
} 10 Personalized POP and Web E-mail Accounts, and
} much more.
} Signup at www.doteasy.com
}
}
}
}

__________________________________
Do you Yahoo!?
Yahoo! Mail - You care about security. So do we.
http://promotions.yahoo.com/new_mail

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 26 15:54:45 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopists,

For a seminar about "Progress in imaging since Van Leeuwenhoeck", I'm
looking for:

Firstly: a photo (and description) of the largest microscope in the world
(it's dimensions, therefor not specifically it's magnification), and not the
particle accelerator of CERN in Swiss (since this is somehow also
'microscope').

Secondly: Websites with new achievements about imaging, here I mean new
technologies like EM, PET-scan, MicroCT, Synchrotron,... compared to normal
brightfield microscopy

Thanks a lot in advance!

Sven Terclavers

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Sven Terclavers
Life Science Imaging - Microscopy
Center for Transgene Technology & Gene Therapy - VIB
Campus Gasthuisberg O&N
Herestraat 49
3000 Leuven
Belgium
Tel.: +32 (0)16 34 61 73
Fax: +32 (0)16 34 59 90
Email: Sven.Terclavers-at-med.kuleuven.ac.be
Intranet: http://debian.med.kuleuven.ac.be
Internet: http://www.kuleuven.ac.be/mcm
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

From MicroscopyL-request-at-ns.microscopy.com Thu Aug 26 16:24:52 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ryan,
Does the SE image change intensity with the emission current
fluctuations? If not, it could simply be a defective metering circuit. Or,
check the following:
1. Poor vacuum in the gun chamber - Are the walls of the gun chamber a
silver-blue color which wipes off easily with some acetone on a cloth? This
indicates a vacuum leak in the gun area, check your "O" rings for dirt, etc.
2. Remover your filament entirely, pump the SEM back down, turn on the HV
and run up the filament current knob to where your filament is normally
peaked and see if (A) you get an emission current above the normal "dark"
current and (B) does it still fluctuate. If the answer is no, I would
suspect vacuum. If Yes, then your gun may be contaminated, a bad HV cable,
or High Voltage Power Supply, or the emission (BIAS) circuit. If you HVPS
is an oil filled tank like the JEOL SEM's, the oil may be old and
contaminated and simply may need to be exchanged with fresh. Good luck!

Gary M. Easton
Scanners Corporation
Independent SEM Service, EDS and SEM Digital Imaging Sales
410-857-7633

----- Original Message -----
} From: "by way of MicroscopyListserver" {ryan.davis-at-hydro.com}
To: {microscopy-at-ns.microscopy.com}
Sent: Thursday, August 26, 2004 12:33 PM

Dear Ryan,
In addition to the suggestions you have already received, a couple of other
things you might check:
1. whiskers on the inside of the hole in the Wehnelt cap. It never hurts to
clean the Wehnelt, anode plate and check just under the anode plate for
contamination.
2. remove the Wehnelt and check the two little copper contacts in the gun
that the legs of the filament engage when you put the filament assembly into
the gun. Make sure they are firm, not bent or broken.
3. Make sure you are not putting the filament into the Wehnelt at right
angles to the proper direction. In my S-3000N, the legs of the filament must
line up with the notches in the Wehnelt, but with another, older Hitachi
SEM, they must be at right angles to the notches. Check your
filament-changing instructions in the manual.
I hope this helps.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "by way of MicroscopyListserver" {ryan.davis-at-hydro.com}
To: {microscopy-at-ns.microscopy.com}
Sent: Thursday, August 26, 2004 9:33 AM

I have received the following responses to my inquirey about care and sectioning of 17 year old paraplast blocks. I cannot begin to repay or even relate the help I have received through the Internet over the past five years or so on microscope issues. These replies are further evidence of the good will of microscopists everywhere. I don't mind expressing my gratitude to a friend, a microscopist who is a wealth of information and advice about every level of microscopy from the most primitive LM to the most advanced electronic varieties, who has offered to provide a microtome which is not being used, for which I will pay shipping. My benefactor, whom I am loathe to identify for fear of embarassing him, has made the use of a lonely phase contrast scope, several remarkable objectives, and other diverse parts available for my teaching and research.

Meanwhile, upon opening the box of wax blocks, I have found a number of slides of previously sectioned material that was not deparaffinized. I don't remember why, in the heat of the moment, when I was packing up to leave the laboratory where I'd been working, I made these particular sections.

Thanks to everyone who has offered advice, as follows:

Damian Neuberger Ph.D.:

Having made more Paraplast embeddings than I care to remember.....
The only thing I can think of is to keep them in a cool dry place;
otherwise, they should last a very long time. The other question
about how to section is an interesting one. You already have a hand
microtome and it might be worth a try although I don't know how it is
to be set up. You have a microtome blade but do you have a handle for
it? If not, it should be a low cost item to acquire and I have the
name of a local place that may have some.

I made a hand microtome using a screw type micrometer gauge, cutting
off the arch portion and mounting the screw portion to a steel
cylinder with a hole in it through which the screw part would push the
tissue sample out by a known amount. The top of the cylinder of
course was ground to a reasonably fine polish. The exposed sample was
then cut using a microtome blade on a handle. I used it primarily for
fresh plant samples that I would sandwich between some soft material
like balsa, or Sambucus pith, cork, etc before inserting it into the
hole.

} From: "Ray Hicks" {rayh-at-fcspress.com}

some plans are given here
http://www.microscopy-uk.org.uk/mag/art97b/microt.html
along the lines of damian's suggestion - if you don't want to destroy
a micrometer, you can buy the moving part (minus the frame) as a
separate component - this gives you a neat cylindrical stub that you
can insert into your tube, and hold it with a grub screw. This thing
is sold as a "micrometer head", slightly more expensive (�7.95) than a
micrometer (�6.95)
http://www.proopsbrothers.com/acatalog/
Online_Catalogue_Micrometers_54.html,
(this is the cheapest I can find them on the web btw, they range up to
250 pounds) but easier to handle I'm sure, also more reproducible than
the heated rod method (these are graduated in 10 micron steps, but you
can estimate smaller steps - although I imagine that the effect of
cutting will cause wedge-shaped sections unless the core you're
cutting is pretty much jammed in the barrel, I made something similar
to make plastic discs from teflon rod, and had to fit a friction screw
to stop the rod being dragged out of the tube by the force of cutting)

as an alternative, the shop at microscopy org has two microtomes (a
hand one -at- 32 pounds, and a rocking one -at- 120 pounds) that might just
be worth exploring... http://www.microscopy-uk.org.uk/full_shop.html

} From: {jb_sanderson-at-yahoo.com}

You are very unlikely to cut good sections on a hand
microtome, and the wax blocks will probably need
softening and then cutting very cold on a modern
microtome with sharp new Feather disposable blades. I
suggest that you try to contact your local
histopathology department for more help at your
hospital.

} From: "Michael Pendleton" {mpendleton-at-mic.tamu.edu}

While I worked at the USDA, I used paraplast blocks for thin
sectioning of insects which were about 10 years old and they still had
active peptides capable of immunocytochemical labelling. The sections
looked very good also. Perhaps it is more important that the
fixatives used worked well because it is probably unlikely that the
paraplast has deteriorated. My guess is the samples will work well if
they were well fixed.

Gordon Couger gcc-at-couger.com:

As a high school teacher in the Pacific you can probably get some
one to donate a microtome if you pay the freight. A wanted message
in the equipment exchange section of the MSA web page would be one
place and wanted messages are free on www.labx.com if you highschool
will give a letter to the person donating the micotome a letter for
a tax deduction it is probably worth more than a used microtome will
sell for.

Of course the freight is going to be a good deal of money on a
microtome.

Frederick Monson:

As far as preserving the blocks, there is only one thing that you will
have to insure. You must try to keep the blocks from temperatures
over 40 degrees C. In a black box, such outside temperatures can
become elevated and cause damage. On the other hand, as long as the
tissues/specimens remain enveloped in the paraffin, you can always
'trim' them out and re-block them before you are ready to section.

On Sat, 14 Aug 2004 17:35:22 +1000
"Alan E. Davis" {aedavis-at-eccomm.com} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I have just received a box full of wax blocks I made about 18 years ago for a study of reproductive biology and zooxanthellae of Millepora platyphylla, fire coral. I don't have a microtome, but thanks to the generosity of a microscopist cleaning out a lab, I have a steel microtome knife. I surely cannot afford a microtome, or especially the shipping, but I remember some plans from some discarded library material some years ago, for microtomes. One used a candle to heat a long metal rod, effectuating a gradual lengthening, for example.
}
} Can anyone suggest whether hand sections are worth while to try of wax blocks. The blocks were made with paraplast. Being several years old, even though I have to assume they are still ok, should I take any special steps with storage or rejuvenation?
}
} I'm afraid to ruin them. The study broke some ground, has not been published, and deserves to be followed through. Even though my notes have been lost through years of sore neglect, typhoons, and ex-spousal neglect, there are some hopeful clues in these specimens that would still be worthwhile, and dates of collection and other information are still recoverable for the most part, through existence of a parallel labelled collection of hard parts, with dates written on them. So... any suggestions on long term care of wax blocks, as well as suggestions for hand-sectioning, would be welcome.
}
} I have a hand microtome, with German inscriptions, that may be useful.
}
} Thank you,
}
} Alan Davis
} Kagman High School
} Saipan, N. Mariana Islands
} aedavis-at-eccomm.com
}

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 27 08:49:06 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have an Amray 1000A currently in use. 1977 or 1978 vintage.
I assume that it is the model that you are referring to as
the "1000". As far as I know all the manuals, charts, etc. are still
in the cabinet
but we have not needed to refer to them in some time so I will check for you.
If you have already received the schematics please let me know.

Pat Connelly
Univ. of Pennsylvania, Dept. of Biology
Philadelphia, PA 19104-6018
215-898-7145

} ----- Original Message -----
} } From: "Ken Converse" {kenconverse-at-qualityimages.biz}
} To: "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com}
} Sent: Wednesday, August 25, 2004 6:18 PM
} Subject: [Microscopy] Amray 1000 schematics
}
} } Does anyone have access to a set of schematics (11" x 17") for the Amray
} } 1000? This is not the 1000B (and 1600 series) with the
} } microprocessor-based
} } mag control, but the original 1000 with analog controls
} } We can copy and return originals or pay copy and shipping costs. Please
} } contact Earl Weltmer at: earlw-at-sbcglobal.net
} } Or me, below.
} }
} } Thanks,
} } Ken Converse
} }
} } owner
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes
} } Since 1981
} } 16 Creek Rd.
} } Delta, PA 17314
} } 717-456-5491
} } Fax 717-456-7996
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} } ___________________________________________________________
} Good to hear someone is still running a 1000, as we have not set ours up
} yet, it and all the documentation is boxed up. I fear it may be behind a few
} thousand pounds of boxes in the warehouse, but I can check on it. we ended
} up with the unit from Honeywell computer plant here in Phoenix.
}
} It would be good to know who else out there is running one of these units. I
} suspect we are a small group. Just have Amray in the title of the message
and it will catch my eye.

} Thanks Ed Sharpe, Archivist for SMECC - - See the Museum's Web Site at
} www.smecc.org
}
} Coury House / SMECC
} 5802 W. Palmaire Ave. Phone 623-435-1522
Glendale Az 85301 USA

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 27 13:33:34 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (watson-at-wi.mit.edu) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, August 27, 2004 at 10:20:20
---------------------------------------------------------------------------

Email: watson-at-wi.mit.edu
Name: Nicki Watson

Organization: Whitehead Institute

Title-Subject: [Microscopy] [Filtered] MEM training schools

Question: Hi all,
I am trying to find the name and web site of the two schools that
offer an associate degree in electron microscopy.
I believe there is one on the west coast, and one in the mid west.
I met a few of the students from those schools at the recent meeting
in Savannah, and I would like to get some more information about
their program, and possibly submit a want ad to their placement
department.
If anyone knows of these schools please let me know.
Thanks
Nicki Watson

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 27 15:14:08 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Aug 27, 2004, at 11:56 AM, by way of MicroscopyListserver wrote:

} I am trying to find the name and web site of the two schools that
} offer an associate degree in electron microscopy.
} I believe there is one on the west coast, and one in the mid west.
} I met a few of the students from those schools at the recent meeting
} in Savannah, and I would like to get some more information about their
} program, and possibly submit a want ad to their placement department.
} If anyone knows of these schools please let me know.
}
Dear Nicki,
Delta College is probably the West Coast school you have in mind. It
was run by Judy Murphy for a long time. I do not know the present
situation with that program, since Judy is no longer affiliated with
it; however, up until Judy left, it was an excellent program, and I
would be certain that any graduate of that program would be exceedingly
well trained.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 27 16:53:52 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nicki:

The training schools are as follows:

Madison Area Technical College
http://matcmadison.edu/electronmicros/

San Joaquin Delta College
http://www.deltacollege.edu/dept/electmicro/index.html

We have hired people from both places and have been very pleased with
the quality of students they turn out.

I hope this helps.

Best regards-

David

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for
Metallogaphy, Crystallography and Electron Microscopy.
Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is
privileged and confidential. This message is intended for the
individual or entity addressed. If you are not the intended recipient,
please do not read, copy or disclose this communication. Notify the
sender of the mistake by calling +1-949-492-2600 and delete this message
from your system.

by way of MicroscopyListserver wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (watson-at-wi.mit.edu) from
} http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
} Friday, August 27, 2004 at 10:20:20
} ---------------------------------------------------------------------------
}
}
} Email: watson-at-wi.mit.edu
} Name: Nicki Watson
}
} Organization: Whitehead Institute
}
} Title-Subject: [Microscopy] [Filtered] MEM training schools
}
} Question: Hi all,
} I am trying to find the name and web site of the two schools that
} offer an associate degree in electron microscopy.
} I believe there is one on the west coast, and one in the mid west.
} I met a few of the students from those schools at the recent meeting
} in Savannah, and I would like to get some more information about their
} program, and possibly submit a want ad to their placement department.
} If anyone knows of these schools please let me know.
} Thanks
} Nicki Watson
}
}
} ---------------------------------------------------------------------------
}
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 27 17:02:33 2004

Contents Retrieved from Microscopy Listserver Archives
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Check out:

http://www.deltacollege.edu/dept/electmicro/

It is Delta College in Stockton, CA.

gary g.

At 11:56 AM 8/27/2004, you wrote:

} Email: watson-at-wi.mit.edu
} Name: Nicki Watson
}
} Organization: Whitehead Institute
}
} Title-Subject: [Microscopy] [Filtered] MEM training schools
}
} Question: Hi all,
} I am trying to find the name and web site of the two schools that offer
} an associate degree in electron microscopy.
} I believe there is one on the west coast, and one in the mid west.
} I met a few of the students from those schools at the recent meeting in
} Savannah, and I would like to get some more information about their
} program, and possibly submit a want ad to their placement department.
} If anyone knows of these schools please let me know.
} Thanks
} Nicki Watson
}
}
} ---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 27 17:14:52 2004

Contents Retrieved from Microscopy Listserver Archives
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Just using Delta will get you to the wrong spot I think its full name is:
San Joaquin Delta College
http://www.deltacollege.org/dept/electmicro/
it is in Stockton Ca.

Jim

James L. Roberts
Firearm & Toolmark Examiner
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 654-2308

James.Roberts-at-mail.co.ventura.ca.us

} } } "Bill Tivol" {tivol-at-caltech.edu} 08/27/04 01:44PM } } }

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On Aug 27, 2004, at 11:56 AM, by way of MicroscopyListserver wrote:

} I am trying to find the name and web site of the two schools that
} offer an associate degree in electron microscopy.
} I believe there is one on the west coast, and one in the mid west.
} I met a few of the students from those schools at the recent meeting
} in Savannah, and I would like to get some more information about their
} program, and possibly submit a want ad to their placement department.
} If anyone knows of these schools please let me know.
}
Dear Nicki,
Delta College is probably the West Coast school you have in mind. It
was run by Judy Murphy for a long time. I do not know the present
situation with that program, since Judy is no longer affiliated with
it; however, up until Judy left, it was an excellent program, and I
would be certain that any graduate of that program would be exceedingly
well trained.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Aug 27 20:26:42 2004

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Glad to know of other users out there!

we also have a strange specimen holder that holds many specimens and you
can rotate them in and out of the beam and switch to another one while still
maintaining the vacuum on the column!

Thanks Ed Sharpe archivist for SMECC

Please check our web site at
http://www.smecc.org
to see other engineering fields, communications and computation stuff we
buy, and by all means when in Arizona drop in and see us.

address:

coury house / smecc
5802 w palmaire ave
glendale az 85301

----- Original Message -----
} From: "Pat Connelly" {psconnel-at-sas.upenn.edu}
To: "ed sharpe" {esharpe-at-uswest.net}
Cc: "Ken Converse" {kenconverse-at-qualityimages.biz} ; "MSA Listserver"
{Microscopy-at-MSA.Microscopy.Com}
Sent: Friday, August 27, 2004 7:09 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (patrick.stallings-at-amd.com) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, August 27, 2004 at 16:28:15
---------------------------------------------------------------------------

Email: patrick.stallings-at-amd.com
Name: Patrick Stallings

Organization: AMD

Title-Subject: [Microscopy] [Filtered] TEM junction stain for FIB prepared
semiconductor samples

Question: All,
I am trying to prepare samples for TEM analysis using focused ion
beam and then using a chemical stain to visually enhance the
semiconductor junction. Does anyone have a recipe that does this
well on FIB samples. I have tried several HNO3:HF solutions with no
real luck.
Thanks,

Patrick Stallings

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Sat Aug 28 08:36:15 2004

Contents Retrieved from Microscopy Listserver Archives
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Greetings,

Listers,

Here is the September 2004 Microscopy Today table of contents. I will close
the subscription list for this issue on Tuesday 7 September 2004.
Microscopists in North America and MSA members anywhere can subscribe for
free. Anyone else may subscribe for US$35 per year (to PARTIALLY cover
postage). All subscriptions at http://www.microscopy-today.com Thank you.

Carmichael: Cells Connected by Tiny Tunnels

Gillies: Microscopy & Microanalytical Support for NASA's Microgravity
Materials Science Programs

Magonov: High-Resolution Imaging with Atomic Force Microscopy

Brown, Nicholls, Royston, & D'Keidek: A Suspended Floor for
High-Performance Electron Microscopy and Nanoscience

Favret and Fuentes: RIMAPS and Variogram Analysis of Barley Leaf Surfaces

Hugo and Cady: Preparation of Geological and Biological TEM Specimens by
Embedding in Sulfur

Armitage: Artifacts from Rapid Microwave Processing of Trematode
Tissues (Ascocotyle pachycystis and leighi)

Neal and Russ: Principal Components Analysis of Multispectral Image Data

Camp, Byun, and Jacob: Electron Microscopy Analysis of Multilamellar
Vesicles Prepared from Synthetic Lipids: A Model System for Studying
Membrane Structure in the Molecular Cell Biology Classroom and Laboratory

Greenhut and Greenhut: Flicker Stereo: Digital 3D Viewing for PC &
Presentation

New and Interesting at Microscopy & Microanalysis 2004
Industry News
NetNotes

Ron Anderson, Editor
Microscopy Today

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 30 07:11:45 2004

Contents Retrieved from Microscopy Listserver Archives
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The midwest school offering an Associate Degree in Electron Microscopy is
Madison Area Technical College. The students that we have worked with
from MATC have been very good. Information is available at:

http://matcmadison.edu/electronmicros/

Joe Neilly, Research Investigator
Abbott Laboratories
R4R9, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027
E-mail: joe.neilly-at-abbott.com

} Email: watson-at-wi.mit.edu
} Name: Nicki Watson
}
} Organization: Whitehead Institute
}
} Title-Subject: [Microscopy] [Filtered] MEM training schools
}
} Question: Hi all,
} I am trying to find the name and web site of the two schools that offer
} an associate degree in electron microscopy.
} I believe there is one on the west coast, and one in the mid west.
} I met a few of the students from those schools at the recent meeting in
} Savannah, and I would like to get some more information about their
} program, and possibly submit a want ad to their placement department.
} If anyone knows of these schools please let me know.
} Thanks
} Nicki Watson
}

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 30 07:50:08 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings all,

I'm posting this on behalf of my McCrone colleague, David Wiley. He's
the managing editor of the online journal, ModernMicroscopy.com, and he
asked me to provide this update to the listserver.

Regards,

Elaine Schumacher
McCrone Associates, Inc.
eschumacher-at-mccrone.com

I would like to take this opportunity to thank everyone who visited or
tried to visit our new online journal www.ModernMicroscopy.com. Many of
you responded because of difficulties accessing the site, and I
appreciate your feedback. I tried to work with our ISP at the time, to
fix the problems with the site. In the end, the decision was made to
move the site to another ISP.

The move has been completed, and I would like to extend another
invitation to everyone. I hope that you will give the site a second
chance, if you haven't already. ModernMicroscopy.com is a peer reviewed
journal featuring articles, reviews and tutorials about microscopy by
some of the most experienced microscopists in the field. We are
actively looking for new topics and ideas from the entire microscopy
community. Submission information can be found on the site.

Regards,

David Wiley
Managing Editor
ModernMicroscopy.com
editor-at-modernmicroscopy.com

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 30 09:53:15 2004

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have a cut-away drawing of a scroll pump?

I give a lecture on vacuum including pumping systems in my SEM class and
have demo pumps that have been disassembled or cut open. However, I do not
have a demo scroll pump, their being relatively new as standard on new
instruments. A good drawing or comparison of features and maintenance
compared to oil rotary pump would be appreciated.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

From MicroscopyL-request-at-ns.microscopy.com Mon Aug 30 12:30:39 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There are diagrams that may be helpful in "High-Vacuum Technology - A
Practical Guide", 2E, M. H. Hablanian, 1997, Marcel Dekker, Inc. pp 190-196.

Michael Zemyan
Schafer Corporation

-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Monday, August 30, 2004 8:16 AM
To: message to: MSA list

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mfein-at-bu.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, August 30, 2004 at 09:56:16
---------------------------------------------------------------------------

Email: mfein-at-bu.edu
Name: Marcia D. Feinberg

Organization: Boston University

Education: Graduate College

Location: Boston, MA , U.S.A.

Question: My Pioloform coated slot grids continue to break under the 6okV TEM beam. I cut and look at serial thin sections and we note that the Pioloform usually starts to break between two adjacent sections. We use a lot of liquid Nitrogen in the scope and it helps somewhat, but we still get breakage. I understand at 60kV the electrons are slower and are heating up the grid, compared to working at higher kV's but because of our low contrast brain tissue, we are forced to work at 60kV. I could try re-coating the grids with a thinner layer of Pioloform but that has some risks and lowers resolution somewhat. We also don't have a carbon coater and choose not to carbon coat because of the risks of messing up our series. Knowing all this, could someone suggest another way we can solve our problem? I thank you very much for your consideration. Marcia Feinberg

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 07:12:30 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

With a number of on-going building modifications here as well as the
potential for relocating my EM Facility. we find ourselves in need of a vibration
testing/monitoring system. What I am hoping to find is a simply system to
plug into a Laptop. Having had various vibration testing done in the past, and
where as I fully acknowledge the quality experience of testing service
providers we just can't afford that kind of expense on a continuing basis. We
are looking to do this in-house, monitor "baseline" building vibrations every
couple of weeks or so.

I have picked up the recommendations for a Wilcoxon 731A/P31
Accelerometer but now I need a compatible PCMCIA spectrum analyzer
interface board and software.

Any recommendations? And yes, vendors may respond directly back to
me.

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 10:05:22 2004

Contents Retrieved from Microscopy Listserver Archives
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We have hired Vibration Engineering Consultants to do site surveys and I
believe they also sell survey equipment that runs from a laptop. You can
find more inforation at:
www.vibeng.com
We have been very happy with their work.

Regards,
Joe Neilly, Research Investigator
Abbott Laboratories
R4R9, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027
E-mail: joe.neilly-at-abbott.com

"Richard Edelmann" {edelmare-at-MUOhio.edu}
08/31/2004 07:34 AM
Please respond to edelmare

To: microscopy-at-MSA.Microscopy.com
cc:
Subject: [Microscopy] Vibration testing system

------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

With a number of on-going building modifications here as
well as the
potential for relocating my EM Facility. we find ourselves in need of a
vibration
testing/monitoring system. What I am hoping to find is a simply system to

plug into a Laptop. Having had various vibration testing done in the
past, and
where as I fully acknowledge the quality experience of testing service
providers we just can't afford that kind of expense on a continuing basis.
We
are looking to do this in-house, monitor "baseline" building vibrations
every
couple of weeks or so.

I have picked up the recommendations for a Wilcoxon
731A/P31
Accelerometer but now I need a compatible PCMCIA spectrum analyzer
interface board and software.

Any recommendations? And yes, vendors may respond
directly back to
me.

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 11:27:16 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Aug 31, 2004, at 5:22 AM, by way of Ask-A-Microscopist wrote:

} Question: My Pioloform coated slot grids continue to break under the
} 6okV TEM beam. I cut and look at serial thin sections and we note that
} the Pioloform usually starts to break between two adjacent sections.
} We use a lot of liquid Nitrogen in the scope and it helps somewhat,
} but we still get breakage. I understand at 60kV the electrons are
} slower and are heating up the grid, compared to working at higher kV's
} but because of our low contrast brain tissue, we are forced to work at
} 60kV. I could try re-coating the grids with a thinner layer of
} Pioloform but that has some risks and lowers resolution somewhat. We
} also don't have a carbon coater and choose not to carbon coat because
} of the risks of messing up our series. Knowing all this, could someone
} suggest another way we can solve our problem? I thank you very much
} for your consideration. Marcia Feinberg
}
Dear Marcia,
You need to conduct heat and charge away from the spot where the
electron beam interacts with the specimen, and, since you do not want
to coat the specimen and the Pioloform is not a good conductor, a
remaining possibility is to lower the dose rate to the point that the
heat and charge will dissipate. Possibly, pre-irradiation of the
entire slot with a low dose could produce enough conductivity in the
Pioloform to overcome the problem. I'd experiment with grids that do
not have valuable material on them to determine what pre-irradiation
and/or dose rate are optimal. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 13:58:03 2004

Contents Retrieved from Microscopy Listserver Archives
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Dear Marcia
Try Formvar instead Pioloform. Carbon coating of the grids (before
attaching sections) also highly recommended (you could bought them from any
major EM suppliers) problems with contrast even using Spurr resin (images
in Spurr for some reason looks less contrasty than from Epon) at 80 kV. I
don't believe lowering accelerating voltage could benefit on very (yes)
thick Pioloform film. Pioloform itself will scatter a lot of electrons, so
your signal (image) would be very noisy and less informative. You could
easily adjust contrast using higher voltage and smaller obj aperture. Low
voltage also damaged your sample, so we are not talking here about better
"resolution" at lower voltage... Sergey

At 07:22 AM 8/31/2004 -0500, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 14:09:56 2004

Contents Retrieved from Microscopy Listserver Archives
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We just bought the Spicer Consulting Analysis System SC11. It is a Laptop
based system to measure vibrations using a Wilcoxon 731A Accelerometer,
AC/DC-fields and Sounds.

You can find more information at http://www.spicerconsulting.com/

Robert

At 08:34 AM 8/31/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

----------
Robert F. Klie, PhD
Goldhaber Distinguished Fellow
Center for Functional Nanomaterials (Bldg.480)
Brookhaven National Laboratory
Upton, NY 11973
Tel. (631) 344-7709
Fax. (631)344-4071

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 15:45:06 2004

Contents Retrieved from Microscopy Listserver Archives
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An inexpensive possibility...

The accelerometer may need a signal conditioner/buffer/amplifier - I have
not checked it's specifications. If a PC sound card meets your frequency
range and resolution requirements, the rest is simple and cheap.

A number of free/shareware programs that will turn your PC into an audio
range spectrum analyzer are available. Adjust the accelerometer/amplifier
output level to be compatible with the sound card "line in" requirement
(typically 1 volt peak, max)and you are there...

Regards,
Woody

Woody White
BWXT Services:
http://www.bwxt.com/bwxt.html
My Site:
http://woody.white.home.att.net

-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.edu]
Sent: Tuesday, August 31, 2004 8:34 AM
To: microscopy-at-MSA.Microscopy.com

With a number of on-going building modifications here as well as the

potential for relocating my EM Facility. we find ourselves in need of a
vibration
testing/monitoring system. What I am hoping to find is a simply system to
plug into a Laptop. Having had various vibration testing done in the past,
and
where as I fully acknowledge the quality experience of testing service
providers we just can't afford that kind of expense on a continuing basis.
We
are looking to do this in-house, monitor "baseline" building vibrations
every
couple of weeks or so.

I have picked up the recommendations for a Wilcoxon 731A/P31
Accelerometer but now I need a compatible PCMCIA spectrum analyzer
interface board and software.

Any recommendations? And yes, vendors may respond directly back
to
me.

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 18:11:33 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kn77-at-uwyo.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, August 31, 2004 at 14:43:11
---------------------------------------------------------------------------

Email: kn77-at-uwyo.edu
Name: Kusum Naithani

Organization: University of Wyoming

Education: Graduate College

Location: Laramie, Wyoming, USA

Question: Hi!
I'm working on plant leaf material (sagebrush). Leaf is covered by minute white hairs and due to this reason I'm not able to find the distribution of stomatal cells. Could you please suggest a way to remove these hairs so that I can see stomatal cells.
2nd question..
I want to measure the size and depth of stomatal cells. Could you please tell me the way to fix leaf in its living conditions.
I would greatly appreciate your help.
Thanks!
Kusum

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 18:13:35 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (donaldawbrey-at-texashealth.org) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, August 31, 2004 at 13:00:40
---------------------------------------------------------------------------

Email: donaldawbrey-at-texashealth.org
Name: Donald G. Awbrey

Organization: Harris Methodist Hospital

Title-Subject: [Microscopy] [Filtered] MSA Certification

Question: Dear MSAnetters,

Is there a MSA certification for TEM? If so, where does one go about looking for information. If any one knows, please contact me.

Thank you in advance,

Donald G. Awbrey, HT(ASCP) QIHC
donaldawbrey-at-texashealth.org

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Aug 31 19:04:58 2004

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Greetings all

What embedding protocol have you used for skin (epidermis and dermis)
into Spurr?

Thanks

Bob Becker

Robert Becker, PhD
Department of Anatomy and Cell Biology (mc512)
Univ IL -at- Chicago
Room 578
808 S Wood St
Chicago, IL 60612-7308
312 996 7215 ph
312 413 0354 fx

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 06:16:27 2004

Contents Retrieved from Microscopy Listserver Archives
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Hello All

I'm a student now considering studies in basics of microscopy to consider a
possible career change.

I'm seeking information on flourescence microscopy, why would someone use
this over other methods, the difference between single photon and two photon
fluorescence . Anyone's experience on laser scanning confocal microscopes (
1photon and 2 photon), is it worth the expense? Although I don't know what
they cost, I am told that they are expensive, does anyone know?

Thank You
Paul Bitetto

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 06:42:33 2004

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Silvia,
There are indeed flat, enclosable embedding cylindrical molds, standard size
#00, with a flat end....on both sides! Just like BEEM capsules. I
get ours from Electron Microscopy Sciences (#70021):

http://www.emsdiasum.com/microscopy/products/preparation/capsule.aspx#70021

These should work great with LR White, though I typically use the #00
gelatin capsules with LR White.

Walter F. Bobrowski
Investigative Pathology
Safety Sciences
Pfizer Global Research & Development
Ann Arbor, MI 48105

TEL: 734-622-7814
FAX: 734-622-3478
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From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 08:33:47 2004

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Kusum,

I don't know a good way to remove the hairs, but for counting and
measuring stomata, it would be easier if any waxy cuticle was removed
during processing. I have done this by using acetone as a dehydrant,
rather than ethanol. In my experience, it cleans up the surface of the
leaf quite well and makes the stomata and other surface features stand
out nicely.

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

-----Original Message-----
} From: by way of Ask-A-Microscopist [mailto:kn77-at-uwyo.edu]
Sent: Tuesday, August 31, 2004 6:34 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kn77-at-uwyo.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 31, 2004 at 14:43:11
------------------------------------------------------------------------
---

Email: kn77-at-uwyo.edu
Name: Kusum Naithani

Organization: University of Wyoming

Education: Graduate College

Location: Laramie, Wyoming, USA

Question: Hi!
I'm working on plant leaf material (sagebrush). Leaf is covered by
minute white hairs and due to this reason I'm not able to find the
distribution of stomatal cells. Could you please suggest a way to remove
these hairs so that I can see stomatal cells.
2nd question..
I want to measure the size and depth of stomatal cells. Could you please
tell me the way to fix leaf in its living conditions. I would greatly
appreciate your help. Thanks! Kusum

------------------------------------------------------------------------
---

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 08:58:41 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mingram-at-rohmhaas.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 1, 2004 at 05:24:13
---------------------------------------------------------------------------

Email: mingram-at-rohmhaas.com
Name: Mike Ingram

Organization: Rohm and Haas

Title-Subject: [Microscopy] [Filtered] MListserver: Registration of SEM's due to X-rays

Question: In Delaware it appears we are required to register our SEM as a X-ray source. Does any know this to be true?

Thanks

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 11:05:14 2004

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Kusum,

You can try removing the trichomes by very gently shaving the leaf.
Hold a razor blade almost vertical, just not touching the leaf
surface, and pull in the direction of the tilt. This requires a
steady hand, and doesn't work on robust trichomes, but might on
sagebrush -- I don't know that plant. The other approach would be to
go at the leaf with the blade near horizontal and again just not
touching the leaf, like shaving your face. But I haven't done that in
so long, I've forgotten how.
You don't need to remove the entire trichome, just most of it, so the
surface is revealed.
The best way to measure the size of the stomatal cells would be with
a light microscope on freshly picked leafs in a room (or chamber) of
the appropriate humidity. Or, same conditions, but make a replica
with dental silicone or one of the replica materials the EM companies
sell. The replica could then be examined in the SEM, or with a light
microscope.

Phil

} Email: kn77-at-uwyo.edu
} Name: Kusum Naithani
}
} Organization: University of Wyoming
}
} Education: Graduate College
}
} Location: Laramie, Wyoming, USA
}
} Question: Hi!
} I'm working on plant leaf material (sagebrush). Leaf is covered by
} minute white hairs and due to this reason I'm not able to find the
} distribution of stomatal cells. Could you please suggest a way to
} remove these hairs so that I can see stomatal cells.
} 2nd question..
} I want to measure the size and depth of stomatal cells. Could you
} please tell me the way to fix leaf in its living conditions.
} I would greatly appreciate your help.
} Thanks!
} Kusum
}
} ---------------------------------------------------------------------------

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 11:16:27 2004

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Hi Paul,
Fluorescence microscopy when you have access to confocal microscope is awesome.
For career, every experimentals fields, particulary with bio tag on the name is
not an easy career from money perspective, but from scientific point of view it
is great.

All the best,

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
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}
} Hello All
}
} I'm a student now considering studies in basics of microscopy to consider a
} possible career change.
}
} I'm seeking information on flourescence microscopy, why would someone use
} this over other methods, the difference between single photon and two photon
} fluorescence . Anyone's experience on laser scanning confocal microscopes (
} 1photon and 2 photon), is it worth the expense? Although I don't know what
} they cost, I am told that they are expensive, does anyone know?
}
} Thank You
} Paul Bitetto
}
}
}
}
}
}
}
}

--
Antoine Blanc, Research Associate
Ecole Polytechnique de Montreal
Chemical Engineering, Mike Buschmann Laboratory
CP 6079, succ. Centre-ville
Montr�al Qc, Canada H3C 3A7
Tel.:514-340-4711 ext.:3212,3336,3337
FAX:514-340-4159
secr�tariat: 514-340-4711 ext.:4984

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 11:44:29 2004

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I'm not sure about Delaware, but this is true for the
State of Michigan. You may want to check your State's
website in the registration or health section for
information.

In the last conversation I had with the state
inspector, the State is considering dropping
registration for scanning electron microscopes because
of the negligible danger from SEMs leaking X-rays.
Since the SEM by all practical purposes cannot operate
unless everything is buttoned up for the vacuum, the
chance for X-ray leakage is just about nil.

Stu Smalinskas, P.E.
SKF USA
Plymouth, Michigan

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Mike wrote:

Question: In Delaware it appears we are required to
register our SEM as a X-ray source. Does any know
this to be true?

Thanks

Email: mingram-at-rohmhaas.com
Name: Mike Ingram
Organization: Rohm and Haas

__________________________________
Do you Yahoo!?
Yahoo! Mail is new and improved - Check it out!
http://promotions.yahoo.com/new_mail

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 12:27:45 2004

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O.k., here is my suggestion for removing the root hairs: Follow any standard
fixation protocol (I know you asked about them as well) but you'll find that
sagebrush does not "wet" very well, so you'll need to add a surfactant like
Kodak Photo-flo or Tween (1 drop / 10ml fixative is generally enough) - if you
are looking to follow up with light microscopy then FAA might work very nicely
for you [FAA = Formalin-Acedic acid-Alcohol Formulation: 50% (or 70%)
ethyl alcohol - 90ml, Glacial Acetic Acid - 5ml, Formalin - 5ml]. In which the
alcohol works well to "wet" the material.

In any case, dehydrate the samples to 50%-100% solvent (EtOH), plunge
freeze in liquid nitrogen, rub the surface of the leaves with a wood stick, pre-
cooled razor blade, plastic bar, etc. This should break all the tricomes off the
leaf surface revealing the stomata. Transfer samples back from liquid
nitrogen and continue with sample prep.

To accurately measure somata depth you will have to section the samples and
look at the cells in cross-section.

Standard fixatation

1) Primary Fixation: 1-2% paraformaldehyde, 2-4% glutaraldehyde, in a
suitable pH buffer (i.e. 6.8-7.2 pH 0.2 M Sodium Phosphate, 0.05 M Sodium
Cacodylate, HEPES, PIPES, etc. ). Fixation for 5-120 min. at room temp. (20-
22 C), normal growth temp (37 C?) or on ice (0-4 C). [50 min. -at- room
temp]. Specimens should be cut as small as possible.

2) Rinse: 4 times -at- 10-15 min. each with the above buffer without
aldehyde fixatives. (Residual aldehydes will bind with OsO4 in secondary
fixation if used.)

*3) Secondary Fixation: 1-2% Osmium tetroxide (OsO4) in full to half
strength buffer used above. Fixation for 2-6 hours at room temperature.
(OsO4 fixation generally not used if immunological staining procedures will be
used.)

*4) Rinse: 4 times -at- 15-20 min. each with distilled water.

5) Dehydration: Generally either absolute ethanol (200 proof) or glass
distilled acetone is used.

% Solvent in water Time

25% 20-30 min
50% 20-30 min
75% 20-30 min
95% 30-60 min
100% 60+ min
100% 60+ min

SEM: CPD samples

TEM: resin embbedd samples

}
} Email: kn77-at-uwyo.edu
} Name: Kusum Naithani
}
} Organization: University of Wyoming
}
} Education: Graduate College
}
} Location: Laramie, Wyoming, USA
}
} Question: Hi!
} I'm working on plant leaf material (sagebrush). Leaf is covered by minute white
} hairs and due to this reason I'm not able to find the distribution of stomatal
} cells. Could you please suggest a way to remove these hairs so that I can see
} stomatal cells. 2nd question.. I want to measure the size and depth of stomatal
} cells. Could you please tell me the way to fix leaf in its living conditions. I
} would greatly appreciate your help. Thanks! Kusum
}
} ---------------------------------------------------------------------------
}

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 12:57:24 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is also true for Illinois. We used to have routine inspections by the
IL Dept of Nuclear Safety, but they felt it was no longer justified for SEMs.

Alan Stone
ASTON

At 12:06 PM 9/1/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 14:02:18 2004

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Mike,
As of July 2000 it was still required. Try contacting

Delaware Health and Social Services
Division of Public Health
Office of Radiation Control
P.O. Box 637
Dover, DE 19903
302-739-3787

Perhaps 4 years later you might be able to find a website for them. From
Stu's reply it sounds like some sanity might be returning concerning SEMs
and x-rays, but I'm not going to go there today.

Good luck,
Ken Converse

owner�
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:mingram-at-rohmhaas.com]
Sent: Wednesday, September 01, 2004 10:21 AM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mingram-at-rohmhaas.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday,
September 1, 2004 at 05:24:13
---------------------------------------------------------------------------

Email: mingram-at-rohmhaas.com
Name: Mike Ingram

Organization: Rohm and Haas

Title-Subject: [Microscopy] [Filtered] MListserver: Registration of SEM's
due to X-rays

Question: In Delaware it appears we are required to register our SEM as a
X-ray source. Does any know this to be true?

Thanks

---------------------------------------------------------------------------

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From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 14:55:47 2004

Contents Retrieved from Microscopy Listserver Archives
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Hi Marcia,

Just some thoughts on your breaking grids:
1. Make sure the Pioloform is absolutely fresh.
2. Can you use a regular mesh grid for additional support?
3. You could obtain carbon coated slot grids from Ladd or some other EM
supplier.

John Arnott

Disclaimer: Ladd Research sells grids, custom coated grids, and the supplies
needed to make them yourself.

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: ladres-at-att.net

----- Original Message -----
} From: "Bill Tivol" {tivol-at-caltech.edu}
To: {microscopy-at-msa.microscopy.com}
Sent: Tuesday, August 31, 2004 12:57 PM

The fall meeting of the Texas Society for Microscopy will be Oct. 21-23
at the Hilton Garden Inn in Allen, TX. We have an exciting schedule
planned including the Thursday workshop "EBSD and FESEM - A Formidable
Combination for Characterization of Semiconductor Materials" to be
presented by Dr. Keith Dicks from Oxford Instruments. The workshop will
take place at Microtech Analytical Labs, Inc., 538 Haggard Street, Suite
402 Plano, TX 75074.
In addition to the Thursday workshop, Oxford Instruments, JEOL and
Microtech Analytical Labs are making the Inca Energy EDX and Inca
Crystal EBSD system on a JEOL 6500F available for demo Tues, Wed and Fri
(Oct. 19-20, 22). This is an opportunity to bring your own samples and
evaluate this technique with one on one contact with the industry
experts. To schedule demo time, contact Mike Crowley with Oxford
Instruments at 512-246-7551 or by email at crowley-at-ma.oxinst.com. Space
is limited so sign up early.

All registration forms and hotel information are available on our web
site: http://www.texasmicroscopy.org/. We look forward to seeing you
in the fall.

Regards,
Jodi Roepsch
Program Chair
972-952-3228, j-roepsch1-at-raytheon.com

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 17:00:06 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (nbauer-at-paulstra.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, September 1, 2004 at 10:11:08
---------------------------------------------------------------------------

Email: nbauer-at-paulstra.com
Name: Nathan Bauer

Organization: Paulstra

Education: Graduate College

Location: Grand Rapids, Mi, USA

Question: Can carbon nano tubes :
1) Carry an electrical current (and if so, how much)?

2) Can they be polorized?

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 18:19:04 2004

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This is not strictly true, Michigan are thinking of stopping the
registration if the instrument is "unmodified" from the manufacturer.
If you have added anything that was not supplied by the manufacturer,
i.e an XEDS system, CL system, viewport or similar then they still want
it registered and tested. We just had our health and safety people
check all our stuff because the Michigan inspector came round and
checked all of our machines (TEMs, SEMs FIBs and XPS).

--
John Mansfield PhD MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143 USA
Phone: (734) 936-3352 FAX (734) 763-2282
Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42� 16' 48" Long. 83� 43' 48"
AIM: thejfmjfm

Home address:
4304 Spring Lake Boulevard
Ann Arbor MI 48108-9657
Phone (734) 994-3096

On Sep 1, 2004, at 1:06 PM, Kestutis Smalinskas wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I'm not sure about Delaware, but this is true for the
} State of Michigan. You may want to check your State's
} website in the registration or health section for
} information.
}
} In the last conversation I had with the state
} inspector, the State is considering dropping
} registration for scanning electron microscopes because
} of the negligible danger from SEMs leaking X-rays.
} Since the SEM by all practical purposes cannot operate
} unless everything is buttoned up for the vacuum, the
} chance for X-ray leakage is just about nil.
}
} Stu Smalinskas, P.E.
} SKF USA
} Plymouth, Michigan
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
} Mike wrote:
}
} Question: In Delaware it appears we are required to
} register our SEM as a X-ray source. Does any know
} this to be true?
}
} Thanks
}
} Email: mingram-at-rohmhaas.com
} Name: Mike Ingram
} Organization: Rohm and Haas
}
}
}
}
}
} __________________________________
} Do you Yahoo!?
} Yahoo! Mail is new and improved - Check it out!
} http://promotions.yahoo.com/new_mail
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 20:52:22 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lotocka-at-acn.waw.pl) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 1, 2004 at 14:49:49
---------------------------------------------------------------------------

Email: lotocka-at-acn.waw.pl
Name: Barbara Lotocka

Organization: Department of Botany, Warsaw Agricultural University

Title-Subject: [Microscopy] MListserver: fixative for lichen

Question: Hello Everyone,

I would be most grateful for any suggestions on a fixation protocol (for transmission electron microscope) optimized for lichens.

I fixed some samples of Cladonia in a fixative that is routinely used for plant samples in my department (paraformaldehyde + glutaraldehyde in sodium cacodylate buffer), but after embedding in epoxy resin the thallus looked shrunken and the section were "scratched" as if the thallus was extremely hard. Perhaps the problem was in dehydration? I used the usual graded series of ethanol and acetone.

With best regards - and hope ;-)
Barbara

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From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 20:53:09 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pzou-at-feico.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 1, 2004 at 20:19:52
---------------------------------------------------------------------------

Email: pzou-at-feico.com
Name: Pei Zou

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Can anybody comment on the image darkening effect as one progressively scans the surface of a sample with a SEM? What are the possible physical causes, and how to reduce the effect?

Any articles that can provide an overview of this phenomenon?

Thanks,

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 1 21:14:28 2004

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Kusum

One possible way to remove hairs that should work is to make a surface replica of the surface using nail varnish or a mounting medium such as Shur Mount and stripping off when dry. On most of the leaf tissues I've worked with it removes hairs, fungi, surface debris but does not damage the surface itself.

Ian

Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz

} } } by way of Ask-A-Microscopist {kn77-at-uwyo.edu} 1/09/2004 11:34:02 a.m. } } }

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kn77-at-uwyo.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, August 31, 2004 at 14:43:11
---------------------------------------------------------------------------

Email: kn77-at-uwyo.edu
Name: Kusum Naithani

Organization: University of Wyoming

Education: Graduate College

Location: Laramie, Wyoming, USA

Question: Hi!
I'm working on plant leaf material (sagebrush). Leaf is covered by minute white hairs and due to this reason I'm not able to find the distribution of stomatal cells. Could you please suggest a way to remove these hairs so that I can see stomatal cells.
2nd question..
I want to measure the size and depth of stomatal cells. Could you please tell me the way to fix leaf in its living conditions.
I would greatly appreciate your help.
Thanks!
Kusum

---------------------------------------------------------------------------

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From MicroscopyL-request-at-ns.microscopy.com Thu Sep 2 01:26:55 2004

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Hi

Anyone out there know of any courses in microscopic morphometry/digital
image analysis - preferably in Europe.

Websites of special interest groups of professional bodies with special
interest sections would also be appreciated.

All the best

Med v�nliga h�lsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Bes�ksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
f�r klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 2 01:54:31 2004

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Dear Pei Zou,

See section 9.10.6 in Goldstein et al.: Scanning electron microscopy and X-ray analysis" Plenum Press 1992. It begins: "A sample subjected to electron bombardment in a diffusion-pumped vacuum gradually becomes ccovered with a contamination layer due to polymerization, under the action of the beam, of organic matter adsorbed on the surface".

Ways to reduce the effect are: clean vacuum, clean sample, cold finger.

Best regards,
Jorgen.

{:::} --- {:::} --- {:::} --- {:::} --- {:::} --- {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:pzou-at-feico.com]
Sent: 2. september 2004 04:16
To: microscopy-at-microscopy.com

---------------------------
Dr Wally H. M�ller
Senior University Research
University of Utrecht, Faculty of Biology
Molecular Cell Biology - Electron Microscopy
Kruyt building, Room West 510
Padualaan 8, 3584 CH Utrecht, The Netherlands
Phone +31 30 2533588 Fax +31 30 2513655
E-mail W.H.Muller-at-bio.uu.nl

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 2 05:16:35 2004

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In a message dated 9/2/04 3:45:16 AM, Gareth.Morgan-at-labmed.ki.se writes:

} Anyone out there know of any courses in microscopic morphometry/digital
} image analysis - preferably in Europe.

Upcoming courses on quantitative image analysis that I will be teaching are:

Sunday October 3 - A one-day tutorial on strategies for quantitative image
analysis will be presented as part of the AOCS Conference on Food Structure and
Quality in Cork, Ireland. Registration for the workshop is available at
{http://www.aocs.org/meetings/fsq/courses.asp}

Tuesday, November 9 - Thursday, November 11 - A three-day hands-on course on
Quantitative Image Analysis will be presented at the University of Missouri,
Columbia, MO. Contact {rosslm-at-missouri.edu} Dr. Lou Ross, Electron Microscopy
Core Facility, W136 Veterinary Medicine, University of Missouri, Columbia, MO
65211-5120, (573) 882-4777, fax 884-5414.

Wednesday, March 16 - Friday, March 18, 2005 - A three-day hands-on course on
Photomicrography and Advanced Image Analysis will be presented at the McCrone
Institute in Chicago. Contact {rweaver-at-mcri.org} Dr. Rob Weaver at the
McCrone Institute, 2820 South Michigan Avenue, Chicago IL 60616, 312-842-7100. A
brief description of the course contents is available at
{http://www.mcri.org/Course_description.html#advdig} their website

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 2 08:17:25 2004

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Colleagues

The August Archives are now on-line at

http://www.microscopy.com/MicroscopyListserver

Nestor
Your Friendly Neighborhood SysOp

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 2 10:50:32 2004

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Kusum & Ian,

For a simple and effective method for making plant surface replicas for
observation by LM or SEM, see my article in the Nov/Dec 2003 issue of
Microscopy Today: Cellulose Acetate Replication of Plant Surfaces for SEM
(plug plug!!). You'll see images of stomata there.

http://www.microscopy-today.com

However, if a plant surface is very thickly populated by a tangled mess of
hairs, or trichomes, then replication may not be possible, as it will be
full of holes left by the trichomes and may tear apart upon attempted
removal from the surface. If the hair density is not too high, even though
holes from trichomes may be present, you may still be able to see enough
surface to get a good sample of the stomata.

--
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-2785 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

"You can learn a lot by observation - just by lookin'!" - Yogi Berra

} Kusum
}
} One possible way to remove hairs that should work is to make a surface replica
} of the surface using nail varnish or a mounting medium such as Shur Mount and
} stripping off when dry. On most of the leaf tissues I've worked with it
} removes hairs, fungi, surface debris but does not damage the surface itself.
}
} Ian
}
}
} Ian Hallett
} HortResearch
} Mt Albert Research Centre, Private Bag 92 169
} Auckland, New Zealand
} Fax +64 9 815 4201
} Telephone +64 9 815 4200
} EMail ihallett-at-hortresearch.co.nz
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted
} by (kn77-at-uwyo.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on
} Tuesday, August 31, 2004 at 14:43:11
} ---------------------------------------------------------------------------
}
} Email: kn77-at-uwyo.edu
} Name: Kusum Naithani
}
} Organization: University of Wyoming
}
} Education: Graduate College
}
} Location: Laramie, Wyoming, USA
}
} Question: Hi!
} I'm working on plant leaf material (sagebrush). Leaf is covered by minute
} white hairs and due to this reason I'm not able to find the distribution of
} stomatal cells. Could you please suggest a way to remove these hairs so that I
} can see stomatal cells.
} 2nd question..
} I want to measure the size and depth of stomatal cells. Could you please tell
} me the way to fix leaf in its living conditions.
} I would greatly appreciate your help.
} Thanks!
} Kusum
}

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 2 12:45:37 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jtd1-at-psu.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 2, 2004 at 10:55:11
---------------------------------------------------------------------------

Email: jtd1-at-psu.edu
Name: Tom Doman

Organization: Penn State University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Our lab is considering various photo printers (} $1K) for production of electron micrographs. Currently the Epson 2200 is the stromg favorite. Are there any recomendations for other printers which we should consider? What are your reasons for the recomended printer?

Thanks in advance!

Tom

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 2 12:46:17 2004

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} Email: walter.bobrowski-at-pfizer.com
} Name: Walt Bobrowski
}
} Organization: Pfizer Global R&D
}
} Title-Subject: [Microscopy] [Filtered] DMP-30 vs. BDMA
}
} Question: Can one substitute BDMA for DMP-30 in a Luft-based epoxy
} resin? If so, what would the proportion be? Currently, I add 2ml
} DMP-30 to 100 ml resin (PolyBed 812, NMA, DDSA). I believe I read
} you can substitute to produce a less viscous mixture, but can't find
} it. Any references appreciated!
}
} Walt -

The only reason that DMP-30 is still around is tradition; by all
means substitute the same amount of BDMA. Viscosities: BDMA, 0.85
cP, DMP-30, 20.5 cP. The quantity that you use is small, so there
won't be a big change in the viscosity of the mix, but DMP-30 is so
viscous that it can actually partition out of the mix during
infiltration! AND DMP-30 is hygroscopic, which leads to more
problems. The original reference is A. Glauert, Proc. RMS 22:264
(1987) and you'll find the data in chapter 6 of Glauert & Lewis,
Biological Specimen Preparation for Transmission Electron Microscopy,
Princeton,1998.

Why not use a less viscous epoxy, such as Spurr (with the new, safer
ERL 4221) or Embed-It? They're both 65 cP, mixed.

--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 2 14:50:47 2004

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So after years of hearing and reading about why BDMA is better than DMP-30,
I switched to BDMA I used it at about 60-80% of the weight of DMP-30 and
got equivalent results using freshly made resin mixes. But I routinely
store my extra resin at -20 C and saw a difference. The BDMA mixtures got
much more viscous (presumably partially polymerized) after 1-2 weeks at -20
compared to the DMP-30 mixtures. So I went back to DMP-30. Whichever one
you use, I strongly advised you begin to dispense it and the other
components by weight. I have a scale in my fume hood and make 50 ml
batches of epoxy resins this way and they are much more consistent and the
mess is significantly less. good luck. tom phillips

01:08 PM 09/02/04 -0500, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 00:24:30 2004

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Dear Tom

I had a look at the archive and this had been discussed a few times in the last few years. Technology is changing fast especially in the digital world. We two would like to know the best printer (quality) out there if there is no money limitation and the best value for money meaning quality prints all editors of journals will be happy with. We got a comment like "the digital images are brilliant but the prints do not do them justice" from a editor.
Please pass all communication to us as well.
Thanks for all the help.

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:jtd1-at-psu.edu]
Sent: Thursday, September 02, 2004 8:08 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jtd1-at-psu.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 2, 2004 at 10:55:11
---------------------------------------------------------------------------

Email: jtd1-at-psu.edu
Name: Tom Doman

Organization: Penn State University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Our lab is considering various photo printers (} $1K) for production of electron micrographs. Currently the Epson 2200 is the stromg favorite. Are there any recomendations for other printers which we should consider? What are your reasons for the recomended printer?

Thanks in advance!

Tom

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 02:43:09 2004

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In the same week there is news of significant change in two of the
companies that were
major players in silver image photography in the 20th century.
Ilford has shed almost half its staff preparative to sale of the
traditional photographic
business while it is still a going concern, allowing the company to
focus on its Swiss digital business.
http://www.channel4.com/news/news_story.jsp?storyId=156722
Agfa has shed its traditional photographic film and consumer imaging
business in a management buyout so that it can "focus on its core
growth markets of Graphic Systems and HealthCare, which are rapidly
going digital"
http://news.agfa.com/corporate/news.nsf/news/F07C0210ECC86EA9C1256EF3004D27CE?opendocument

Events like these, and Kodak's announcement earlier this year that it
would cease the production of its poneering APS cameras (though not of
films) underline the fragility of the conventional photographic
market in the face of the growth of digital imaging.

Which begs the question "can we rely on the continued availability of
EM film", and if not, how long have we got
to plan for the conversion to digital?

Dr. Chris Jeffree
University of Edinburgh
Schoolof Biological Sciences

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 05:34:31 2004

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If money were no object? We have been using the Fujifilm 3000 Pictrography
digital printer (it is truly a mini wet-lab). The latest model is the 4500:

http://www.fujifilm.com/JSP/fuji/epartners/PRNewsDetail.jsp?DBID=NEWS_547164
&CAT_ID=233840

These units are designed to print only photo-quality and they do it well. As
we have progressed towards keeping documents (including images) electronic
and have thrown out the darkroom, and due to cost per print ($4.00/8x10),
we only use the Pictrography to print images for manuscripts. If colleagues
want review-quality, they either must review on their computer monitor or
send image files to a B&W/Color LaserJet (yeah, lousy quality, but it's only
for review).

Best regards,

Walter F. Bobrowski
Investigative Pathology
Safety Sciences
Pfizer Global Research & Development
Ann Arbor, MI 48105

TEL: 734-622-7814
FAX: 734-622-3478
Mobile: 734-646-0502

-----Original Message-----
} From: Coetzee, Mr S. H Physics Science [mailto:COETZEES-at-mopipi.ub.bw]
Sent: Friday, September 03, 2004 1:51 AM
To: by way of MicroscopyListserver
Cc: microscopy-at-microscopy.com

Dear Tom

I had a look at the archive and this had been discussed a few times in the
last few years. Technology is changing fast especially in the digital
world. We two would like to know the best printer (quality) out there if
there is no money limitation and the best value for money meaning quality
prints all editors of journals will be happy with. We got a comment like
"the digital images are brilliant but the prints do not do them justice"
from a editor.
Please pass all communication to us as well.
Thanks for all the help.

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:jtd1-at-psu.edu]
Sent: Thursday, September 02, 2004 8:08 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jtd1-at-psu.edu) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday,
September 2, 2004 at 10:55:11
---------------------------------------------------------------------------

Email: jtd1-at-psu.edu
Name: Tom Doman

Organization: Penn State University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Our lab is considering various photo printers (} $1K) for
production of electron micrographs. Currently the Epson 2200 is the stromg
favorite. Are there any recomendations for other printers which we should
consider? What are your reasons for the recomended printer?

Thanks in advance!

Tom

---------------------------------------------------------------------------

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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 05:45:11 2004

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On the Vibration testing, the kit is available from various companies but is
expensive.
We bought a complete unit from spicer consulting
http://www.spicerconsulting.com/
Their SC11 kit costs around $10,000 but that does everything.

However there are other products available. (
http://www.predictech.com/CM/PT908.htm ) not sure though that they will be
sensitive enough.

Luc Harmsen
ANASPEC South Africa

Tel: +27 11 794 8340
Fax: +27 11 794 8349
Mobile: +27 82 4459 003
Email: luc-at-anaspec.co.za
www.anaspec.co.za

P.O. Box 2561
Honeydew 2040
Gauteng, South Africa
-----Original Message-----
} From: White, Woody N. [mailto:nwwhite-at-bwxt.com]
Sent: 31 August 2004 11:02
To: 'edelmare-at-MUOhio.edu'; MicroscopyListServer

An inexpensive possibility...

The accelerometer may need a signal conditioner/buffer/amplifier - I have
not checked it's specifications. If a PC sound card meets your frequency
range and resolution requirements, the rest is simple and cheap.

A number of free/shareware programs that will turn your PC into an audio
range spectrum analyzer are available. Adjust the accelerometer/amplifier
output level to be compatible with the sound card "line in" requirement
(typically 1 volt peak, max)and you are there...

Regards,
Woody

Woody White
BWXT Services:
http://www.bwxt.com/bwxt.html
My Site:
http://woody.white.home.att.net

-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.edu]
Sent: Tuesday, August 31, 2004 8:34 AM
To: microscopy-at-MSA.Microscopy.com

With a number of on-going building modifications here as well as the

potential for relocating my EM Facility. we find ourselves in need of a
vibration
testing/monitoring system. What I am hoping to find is a simply system to
plug into a Laptop. Having had various vibration testing done in the past,
and
where as I fully acknowledge the quality experience of testing service
providers we just can't afford that kind of expense on a continuing basis.
We
are looking to do this in-house, monitor "baseline" building vibrations
every
couple of weeks or so.

I have picked up the recommendations for a Wilcoxon 731A/P31
Accelerometer but now I need a compatible PCMCIA spectrum analyzer
interface board and software.

Any recommendations? And yes, vendors may respond directly back
to
me.

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 07:36:56 2004

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Group,
In my view, simple inkjet printers do an extraordinary job.
You can get one for 200 us dollars (or more or less depending on
features). What is important is that the quality of the output is
determined by the paper you put in (and some software toggles in the
printer driver). So, there is a range of paper from high quality
photo paper to zerox paper, with the cost per print scaling
appropriately (and the time per print likewise). This makes it easy
to get low, intermediate, high quality output on the same printer,
with little fuss. And I have to say that for monochrome prints, the
highest quality settings/paper produce prints equal to anything I
have seen from the wetlab type of printers mentioned below. And even
for color the differences are pretty small.
Another issue is networking. The inexpensive inkjet that I
have is not network-able (that's an intersting word) and I don't know
if the more expensive ones give you that option.

My two pixels,
Tobias

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 08:13:25 2004

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I have already starting campaigning for a digital camera
for our TEM!

I saw an advert in Microscopy and Analysis for a company
which is (presumably) starting making TEM film as others
leave the market.

Kodak did say recently on this listserver that they have no
plans to drop EM film.

Dave

On Fri, 03 Sep 2004 09:05:45 +0100 Chris Jeffree
{c.jeffree-at-ed.ac.uk} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} In the same week there is news of significant change in two of the
} companies that were
} major players in silver image photography in the 20th century.
} Ilford has shed almost half its staff preparative to sale of the
} traditional photographic
} business while it is still a going concern, allowing the company to
} focus on its Swiss digital business.
} http://www.channel4.com/news/news_story.jsp?storyId=156722
} Agfa has shed its traditional photographic film and consumer imaging
} business in a management buyout so that it can "focus on its core
} growth markets of Graphic Systems and HealthCare, which are rapidly
} going digital"
} http://news.agfa.com/corporate/news.nsf/news/F07C0210ECC86EA9C1256EF3004D27CE?opendocument
}
} Events like these, and Kodak's announcement earlier this year that it
} would cease the production of its poneering APS cameras (though not of
} films) underline the fragility of the conventional photographic
} market in the face of the growth of digital imaging.
}
} Which begs the question "can we rely on the continued availability of
} EM film", and if not, how long have we got
} to plan for the conversion to digital?
}
}
} Dr. Chris Jeffree
} University of Edinburgh
} Schoolof Biological Sciences
}
}
}
}
} This incoming email to UWE has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"

This email has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 09:21:02 2004

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Kodak has included a positive statement on its continued commitment to film
products in its contribution to the "New and Interesting at M&M 2004"
section of the September issue of Microscopy Today, at the printer now and
in your mail boxes starting in a week or so.

Ron Anderson, MT Editor

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Friday, September 03, 2004 4:06 AM
To: microscopy-at-msa.microscopy.com

In the same week there is news of significant change in two of the
companies that were
major players in silver image photography in the 20th century.
Ilford has shed almost half its staff preparative to sale of the
traditional photographic
business while it is still a going concern, allowing the company to
focus on its Swiss digital business.
http://www.channel4.com/news/news_story.jsp?storyId=156722
Agfa has shed its traditional photographic film and consumer imaging
business in a management buyout so that it can "focus on its core
growth markets of Graphic Systems and HealthCare, which are rapidly
going digital"
http://news.agfa.com/corporate/news.nsf/news/F07C0210ECC86EA9C1256EF3004D27C
E?opendocument

Events like these, and Kodak's announcement earlier this year that it
would cease the production of its poneering APS cameras (though not of
films) underline the fragility of the conventional photographic
market in the face of the growth of digital imaging.

Which begs the question "can we rely on the continued availability of
EM film", and if not, how long have we got
to plan for the conversion to digital?

Dr. Chris Jeffree
University of Edinburgh
Schoolof Biological Sciences

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 10:40:02 2004

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Hello,

I recently learned that Serco-Ilford is no more and I need service for my
2150RC. Does anyone know of anyone serving the New England area? Thanks in
advance.

Mary

Mary McKee
Program in Membrane Biology
MGH-Charlestown
(617)726-3696
--

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 11:18:33 2004

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I freeze with DMP-30 in the tube. Generally I aliquot them so they are
only thawed once. They are certainly good for a couple of weeks this way
and longer in many cases. I make all my resin by weight (typically 20 g
Embed 812, 10 g DDSA, 10 g NMA, and 0.6 g DMP-30) in a 50 ml plastic
disposable tube and shake vigorously until well mixed. We used 0.8 g BDMA
in place of DMP-30 in this formulation and saw no difference in cutting
quality but did have the storage problem. The viscosity of the DDSA and
NMA and Embed 812 is high and requires vigorous shaking regardless of the
catylst so I don't see lower viscosity of BDMA as significant if you are
measuring by weight. If you measure by volume, a viscous solution will be
tougher to accurately measure and deliver and the percent error will be
much higher for the small volume BDMA or DMP-30 component. I have never
ever seen the DMP-30 come out of solution such as Caroline Schooley
suggests in her e-mail; if this happens I would suspect improper
mixing. My use of DMP-30 is based on careful consideration and comparison
with BDMA and not on tradition. I used BDMA exclusively for over 1 year
and then switched back so I think I gave it a fair shot.

At 10:22 AM 09/03/04 -0600, you wrote:
} I have a question about freezing the resin mixtures - you freeze them with
} the accelerator already added? How many freeze/thaw cycles can they stand?
} Or, do you aliquot them to store and thaw only once?? I was taught to
} store mine without DMP-30....if they'll last OK with it in, I'm all for it!
}
} Thanks,
}
} Tamara
}
} On Thu, 2 Sep 2004, Tom Phillips wrote:
}
} }
} }
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 11:35:01 2004

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For a less viscous mixture, you could try one percent DMP-30 instead of
two percent. The final cure might not be quite so hard, though, so
depending on your tissue this may or may not help.

Lesley Weston.

----- Original Message -----
} From: walter.bobrowski-at-pfizer.com (by way of MicroscopyListserver)
Sent: 9/2/2004 11:08:46 AM
To: microscopy-at-microscopy.com

} -
}
} I freeze with DMP-30 in the tube. Generally I aliquot them so they
} are only thawed once. They are certainly good for a couple of weeks
} this way and longer in many cases. I make all my resin by weight
} (typically 20 g Embed 812, 10 g DDSA, 10 g NMA, and 0.6 g DMP-30) in
} a 50 ml plastic disposable tube and shake vigorously until well
} mixed. We used 0.8 g BDMA in place of DMP-30 in this formulation
} and saw no difference in cutting quality but did have the storage
} problem. The viscosity of the DDSA and NMA and Embed 812 is high
} and requires vigorous shaking regardless of the catylst so I don't
} see lower viscosity of BDMA as significant if you are measuring by
} weight. If you measure by volume, a viscous solution will be tougher
} to accurately measure and deliver and the percent error will be much
} higher for the small volume BDMA or DMP-30 component. I have never
} ever seen the DMP-30 come out of solution such as Caroline Schooley
} suggests in her e-mail; if this happens I would suspect improper
} mixing. My use of DMP-30 is based on careful consideration and
} comparison with BDMA and not on tradition. I used BDMA exclusively
} for over 1 year and then switched back so I think I gave it a fair
} shot.

You missed my point, Tom; when I said that the DMP-30 can partition
during infiltration, I meant that it doesn't enter the tissue as
rapidly as the other resin components. The symptom is soft tissue in
a normal, hard block. I agree with you that the high DMP-30
viscosity is going to have little effect on the viscosity of the
mixed epoxy.

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 15:21:20 2004

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Do you have evidence that the DMP-30 doesn't get in or is that your
hypothesis to explain the problem? How do you know it is a sign that the
DMP-30 didn't get in and not the complete mixture or one of the equally
viscous components? I find it hard to believe the DMP-30 differentially
inflitrates. In addition, the DMP-30 (and BDMA) has probably already begun
to react with the other components and would be "carried" in covalently
bound to the one of them. Poor infiltration of the entire mix would (and
does) result in the symptom you describe. Take a resin mix made with BDMA
and do a short infiltration on a tough to infiltrate tissue like maize
endosperm and the center will be softer than the perimeter and free resin
regions. BDMA is frequently touted as superior due to its lower viscosity
and less hydrophilic. It is lower viscosity but I don't see that as a
problem or benefit. I have no data on the relative hydroscopic properties
but I have never had a bottle of DMP-30 go bad on me in the 25 years I have
been doing TEM. Maybe I use my bottles up before they goes bad. We do
close it up promptly after using but I assume most labs do. I have nothing
against BDMA if one is making the resin up fresh each time (which is the
best practice regardless of whether you use BDMA or DMP-30). But for our
routine samples, we commonly make 40-50 gm batches and store the resin at
-20 C for 1-3 weeks. This works when we use DMP-30 but not with BDMA. I
agree that lots of what microscopists do is because of "tradition" but the
selection of DMP-30 can be the result of a careful, reasoned and
experimentally tested decision process. Tom Phillips

At 01:21 PM 09/03/04 -0700, you wrote:
} } -
} }
} } I freeze with DMP-30 in the tube. Generally I aliquot them so they are
} } only thawed once. They are certainly good for a couple of weeks this way
} } and longer in many cases. I make all my resin by weight (typically 20 g
} } Embed 812, 10 g DDSA, 10 g NMA, and 0.6 g DMP-30) in a 50 ml plastic
} } disposable tube and shake vigorously until well mixed. We used 0.8 g
} } BDMA in place of DMP-30 in this formulation and saw no difference in
} } cutting quality but did have the storage problem. The viscosity of the
} } DDSA and NMA and Embed 812 is high and requires vigorous shaking
} } regardless of the catylst so I don't see lower viscosity of BDMA as
} } significant if you are measuring by weight. If you measure by volume, a
} } viscous solution will be tougher to accurately measure and deliver and
} } the percent error will be much higher for the small volume BDMA or DMP-30
} } component. I have never ever seen the DMP-30 come out of solution such
} } as Caroline Schooley suggests in her e-mail; if this happens I would
} } suspect improper mixing. My use of DMP-30 is based on careful
} } consideration and comparison with BDMA and not on tradition. I used BDMA
} } exclusively for over 1 year and then switched back so I think I gave it a
} } fair shot.
}
} You missed my point, Tom; when I said that the DMP-30 can partition during
} infiltration, I meant that it doesn't enter the tissue as rapidly as the
} other resin components. The symptom is soft tissue in a normal, hard
} block. I agree with you that the high DMP-30 viscosity is going to have
} little effect on the viscosity of the mixed epoxy.
}
} Caroline
} --
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
} Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 20:08:29 2004

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I'm looking for sources of acoustic/anachoic
blocks of foam to reduce SEM room interference.

These are like standard anechoic chamber panels.
I'm having trouble getting above 400KX without
mechanical noise from the scroll pump that is
located in a separate room. The acoustical
isolation from one room to the other is not
all that great, I suppose.

Does anyone have experience with suppliers of
these panels? I'd like to glue them to drywall.

Supplier responses are welcomed as off-line
messages.

gary g.

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 3 21:05:34 2004

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Gary,

You can try http://www.illbruck-sonex.com/ for what you need. I'm familiar
with this product when we used it at the High Voltage EM lab at UW Madison.
Worked well.

Damian Neuberger

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Friday, September 03, 2004 8:31 PM
To: MSA listserver

I'm looking for sources of acoustic/anachoic
blocks of foam to reduce SEM room interference.

These are like standard anechoic chamber panels.
I'm having trouble getting above 400KX without
mechanical noise from the scroll pump that is
located in a separate room. The acoustical
isolation from one room to the other is not
all that great, I suppose.

Does anyone have experience with suppliers of
these panels? I'd like to glue them to drywall.

Supplier responses are welcomed as off-line
messages.

gary g.

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 4 07:05:23 2004

Contents Retrieved from Microscopy Listserver Archives
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Dear Researchers:

��We use a Polaroid SprintScan 45 multi-format film scanner to
scan our EM negatives. It has been a work horse for us for 7 years, and
the hardware is still in a good shape. However, We lost the original
disk with driver (for Mac) on it and we could not get support from the
company anymore because Polaroid has discontinued scanner
business.�Does anyone out there have the same scanner and would be
willing to lend us the driver software?�Our machine is currently down
because the computer could not locate it even though the scanner was on
and all cables were connected.�We need to re-install the driver as the
first trouble shooting step.

��If we can not find a driver, we might have to purchase a new
film scanner.�Does anyone has a recommendation on makes and models?

��Thank you very much in advance.

��
Hong
Emory EM
hyi-at-emory.edu

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 4 11:39:26 2004

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Bill Robertson in his reply posting pointed out
a very good point about wall mass. My initial
thoughts were to put the acoustic tiles in the SEM room.
But the noise is more likely coming from the adjacent
room where the scroll pump and specimen interchange
pump are located. The specimen interchange pump
is not at issue. The scroll pump is an Edwards XDS 10.
Based on limited experience, it does not sound like
it is running as it should. Vacuum is fine but the
sound is odd. It makes a mechanical knocking noise.
I'm told that either these pumps work almost forever
when new or die quickly. Sounds like I have the latter.

In either case, wall mass has a high probability
of attention. The intervening wall is standard
drywall with 2x4 studs. There is no insulation
in the wall. I'm thinking of having liquid foam
insulation put in the wall cells and seeing if that
helps. In the mean time, the plinth anti-vibration
system is not exactly right and will get adjusted
shortly. then, based on how that turns out, acoustic
tiles in the pump room may be a good solution. If that
does not help much, then foam insulation.

The chiller is in the same room as the SEM but turning
it off makes no change in image noise. So the noise
is external.

Thanks for the replies. Will work on this in the
next couple of weeks.

gary g.

At 08:38 AM 9/4/2004, you wrote:
} Gary,
}
} They can be pricey. One reason is that most jurisdictions require that
} insulation
} applied to an open wall surface be flame proof. Not only that, the
} adhesive used
} to apply it must also be flame proof, per the fire department and building
} codes.
} (Recall the disasterous fire in the Rhode Island night club a couple years
} back).
} Some years ago I found that the material required behind an XRD system in
} a small
} room ran several hundred dollars for a half dozen panels. However, I
} found that I
} could get a big improvement by placing a small number of them so as to
} block the
} noise at the source, rather than covering the more distant wall
} surfaces. Buy a
} small number to try out, or experiment with packing foam. Have an
} assistant hold
} them in different locations so as to block acoustic reflections. You may
} find that
} suspending one from the ceiling in the plane of the instrument helps alot with
} noise at the position where the operator sits.
}
} John Twilley
}
} Gary Gaugler wrote:
}
} }
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -------------------------------------------------------------------------------
} }
} } I'm looking for sources of acoustic/anachoic
} } blocks of foam to reduce SEM room interference.
} }
} } These are like standard anechoic chamber panels.
} } I'm having trouble getting above 400KX without
} } mechanical noise from the scroll pump that is
} } located in a separate room. The acoustical
} } isolation from one room to the other is not
} } all that great, I suppose.
} }
} } Does anyone have experience with suppliers of
} } these panels? I'd like to glue them to drywall.
} }
} } Supplier responses are welcomed as off-line
} } messages.
} }
} } gary g.

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 4 11:44:55 2004

Contents Retrieved from Microscopy Listserver Archives
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I was a fan of Epson photo printers for quite
some time. Notable was the 890 & 980. It is a
small format printer compared to the 2200.
I recently (a year or so ago) bought a Epson Stylus
Photo 2000. It lasted about six months and
then jammed constantly. In-warranty customer
service and any idea of repair was on a wish list.
It never happened. The printer was scrapped.

The 2200 may have solved teething problems
with large format printers. However, the 2000
was VERY slooooow using photo paper. When it
worked, the results were stunning. Many times
(too many) it would stop printing 1/4 or 1/2
way through the print and just die. The job
hung (Win2K Pro) and had to be restarted with
a new sheet of paper.

The Epson and Canon small format printers seem
to do a better, more reliable job. As a result
of being burned by Epson, I now take print jobs
to a local service bureau. they do a very nice
job for not much cost. These are mostly for
24" x 48" glossy mounted prints. Small ones
are done on my HP 4550 color laser printer.
If the color gamut is matched well between
the monitor and Photoshop, the HP does a nice job
for reports. For transparencies (not much used
any longer), the Kodak dye sub is excellent.

Let us know what you find. There are a lot
of options. Also, check out the Ethernet print
servers that will connect a non-network printer
to a LAN and allow all to use it. HP and others
make these. they usually cost about $100 or so.

gary g.

At 11:08 AM 9/2/2004, you wrote:

} Email: jtd1-at-psu.edu
} Name: Tom Doman
}
} Organization: Penn State University
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Our lab is considering various photo printers (} $1K) for
} production of electron micrographs. Currently the Epson 2200 is the stromg
} favorite. Are there any recomendations for other printers which we should
} consider? What are your reasons for the recomended printer?
}
} Thanks in advance!
}
} Tom
}
} ---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 4 13:33:46 2004

Contents Retrieved from Microscopy Listserver Archives
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From your description of the sound coming from your pump, it sounds
like your issue might be low frequency. For low frequencies, acoustic
tiles and the like are not very effective. The strategy for reducing
high frequencies is to absorb the energy, and usually involves foam or
fibruous materials that will vibrate and dissipate the energy. For low
frequencies, the strategy is to block/reflect the energy, and this
requires rigidity and mass. The best is a very solid wall, but there
are also a number of lead-backed sheet materials, either separately or
in combination with absorbers. If you have access to a McMaster Carr
catalog, I suggest you check out "Sound Absorbers" (or visit their web
site at www.mcmaster.com and do a keyboard search for "sound". There
is also a helpful summary on page 3266 of their online catalog which
explains the various types and ratings systems. (No financial interest
in McMaster Carr, but use them all the time.)
Fred Schamber
ASPEX, LLC

Gary Gaugler wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
}
} Bill Robertson in his reply posting pointed out
} a very good point about wall mass. My initial
} thoughts were to put the acoustic tiles in the SEM room.
} But the noise is more likely coming from the adjacent
} room where the scroll pump and specimen interchange
} pump are located. The specimen interchange pump
} is not at issue. The scroll pump is an Edwards XDS 10.
} Based on limited experience, it does not sound like
} it is running as it should. Vacuum is fine but the
} sound is odd. It makes a mechanical knocking noise.
} I'm told that either these pumps work almost forever
} when new or die quickly. Sounds like I have the latter.
}
} In either case, wall mass has a high probability
} of attention. The intervening wall is standard
} drywall with 2x4 studs. There is no insulation
} in the wall. I'm thinking of having liquid foam
} insulation put in the wall cells and seeing if that
} helps. In the mean time, the plinth anti-vibration
} system is not exactly right and will get adjusted
} shortly. then, based on how that turns out, acoustic
} tiles in the pump room may be a good solution. If that
} does not help much, then foam insulation.
}
} The chiller is in the same room as the SEM but turning
} it off makes no change in image noise. So the noise
} is external.
}
} Thanks for the replies. Will work on this in the
} next couple of weeks.
}
} gary g.
}
}
} At 08:38 AM 9/4/2004, you wrote:
}
} } Gary,
} }
} } They can be pricey. One reason is that most jurisdictions require
} } that insulation
} } applied to an open wall surface be flame proof. Not only that, the
} } adhesive used
} } to apply it must also be flame proof, per the fire department and
} } building codes.
} } (Recall the disasterous fire in the Rhode Island night club a couple
} } years back).
} } Some years ago I found that the material required behind an XRD
} } system in a small
} } room ran several hundred dollars for a half dozen panels. However, I
} } found that I
} } could get a big improvement by placing a small number of them so as
} } to block the
} } noise at the source, rather than covering the more distant wall
} } surfaces. Buy a
} } small number to try out, or experiment with packing foam. Have an
} } assistant hold
} } them in different locations so as to block acoustic reflections. You
} } may find that
} } suspending one from the ceiling in the plane of the instrument helps
} } alot with
} } noise at the position where the operator sits.
} }
} } John Twilley
} }
} } Gary Gaugler wrote:
} }
} } }
} } ------------------------------------------------------------------------------
} }
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } -------------------------------------------------------------------------------
} }
} } }
} } } I'm looking for sources of acoustic/anachoic
} } } blocks of foam to reduce SEM room interference.
} } }
} } } These are like standard anechoic chamber panels.
} } } I'm having trouble getting above 400KX without
} } } mechanical noise from the scroll pump that is
} } } located in a separate room. The acoustical
} } } isolation from one room to the other is not
} } } all that great, I suppose.
} } }
} } } Does anyone have experience with suppliers of
} } } these panels? I'd like to glue them to drywall.
} } }
} } } Supplier responses are welcomed as off-line
} } } messages.
} } }
} } } gary g.
}
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Sun Sep 5 11:32:17 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jbarclay-at-southpointresources.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday, September 5, 2004 at 08:40:08
---------------------------------------------------------------------------

Email: jbarclay-at-southpointresources.com
Name: Jim Barclay

Organization: Calgary, Alberta

Title-Subject: [Microscopy] [Filtered] Wild Heerbrug M5A microscope & camera attachment

Question: I am trying to see if I can find some type of adaptor that will fit my older Wild Heerbrug M5A binocular microscope (15-20 years old?) and allow me to fit a digital camera to the scope. I would need some type of beam splitter that would continue to allow simultaneosu viewing of samples while also allowing occasional photo taking.

I have already contacted and am waiting for reply from a Leica Microsystems representative which owns the Wild Leitz brand.

Thank you for listening to a newbie to this board. Any suggestions welcome.

JB.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Sun Sep 5 17:01:07 2004

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On Sep 3, 2004, at 6:30 PM, Gary Gaugler wrote:

} I'm looking for sources of acoustic/anachoic
} blocks of foam to reduce SEM room interference.
}
} These are like standard anechoic chamber panels.
} I'm having trouble getting above 400KX without
} mechanical noise from the scroll pump that is
} located in a separate room. The acoustical
} isolation from one room to the other is not
} all that great, I suppose.
}
} Does anyone have experience with suppliers of
} these panels? I'd like to glue them to drywall.
}
} Supplier responses are welcomed as off-line
} messages.
}
Dear Gary,
Fred has it right about the difference between low and high
frequencies. The depth of the invaginations in anechoic panels has to
be 1/4 of the wavelength of the sound in order to be effective, so for
low frequencies, the panels could take up the entire room, leaving no
space for the equipment.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Sun Sep 5 18:43:56 2004

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I don't have any direct way of knowing whether the
noise is high or low frequency. It only shows up
above 60KX as sinusoids on the edges of specimen
details.

I can probably use FFT to compute the frequency.
It is consistent.

gary g.

At 03:34 PM 9/5/2004, you wrote:

} On Sep 3, 2004, at 6:30 PM, Gary Gaugler wrote:
}
} } I'm looking for sources of acoustic/anachoic
} } blocks of foam to reduce SEM room interference.
} }
} } These are like standard anechoic chamber panels.
} } I'm having trouble getting above 400KX without
} } mechanical noise from the scroll pump that is
} } located in a separate room. The acoustical
} } isolation from one room to the other is not
} } all that great, I suppose.
} }
} } Does anyone have experience with suppliers of
} } these panels? I'd like to glue them to drywall.
} }
} } Supplier responses are welcomed as off-line
} } messages.
} Dear Gary,
} Fred has it right about the difference between low and high
} frequencies. The depth of the invaginations in anechoic panels has to be
} 1/4 of the wavelength of the sound in order to be effective, so for low
} frequencies, the panels could take up the entire room, leaving no space
} for the equipment.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}

From MicroscopyL-request-at-ns.microscopy.com Sun Sep 5 23:13:35 2004

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If you are building a wall that may transmuted vibration making the
wall a 6 inch wall with double 4 inch studs that are every 8 inches
with every other stud supporting opposite faces of the wall. If you
use a sound deadening blanket widen the wall to leave room to weave
the padding between the studs with out compressing it.

Two layers of 5/8 inch gypsum wall board are generaly considered
fire proof enough for university buildings at Oklahoma State. The
last I knew there were no paints that could be used on anything but
aluminum to increase their fire rating. But that has been a while.
In actual practice there are paints that improve the fire resistance
of anything but last I knew it changed so much with age that for
anything but metal it was too unpredictable to approve.

Gordon
Gordon Couger gcc-at-couger.com

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward any links or information you think might be useful to
others.
Microscope Manual at www.science-info.org

} From: "Gary Gaugler" {gary-at-gaugler.com}
:
: Bill Robertson in his reply posting pointed out
: a very good point about wall mass. My initial
: thoughts were to put the acoustic tiles in the SEM room.
: But the noise is more likely coming from the adjacent
: room where the scroll pump and specimen interchange
: pump are located. The specimen interchange pump
: is not at issue. The scroll pump is an Edwards XDS 10.
: Based on limited experience, it does not sound like
: it is running as it should. Vacuum is fine but the
: sound is odd. It makes a mechanical knocking noise.
: I'm told that either these pumps work almost forever
: when new or die quickly. Sounds like I have the latter.
:
: In either case, wall mass has a high probability
: of attention. The intervening wall is standard
: drywall with 2x4 studs. There is no insulation
: in the wall. I'm thinking of having liquid foam
: insulation put in the wall cells and seeing if that
: helps. In the mean time, the plinth anti-vibration
: system is not exactly right and will get adjusted
: shortly. then, based on how that turns out, acoustic
: tiles in the pump room may be a good solution. If that
: does not help much, then foam insulation.
:
: The chiller is in the same room as the SEM but turning
: it off makes no change in image noise. So the noise
: is external.
:
: Thanks for the replies. Will work on this in the
: next couple of weeks.
:
: gary g.
:
:
: At 08:38 AM 9/4/2004, you wrote:
: } Gary,
: }
: } They can be pricey. One reason is that most jurisdictions
require that
: } insulation
: } applied to an open wall surface be flame proof. Not only that,
the
: } adhesive used
: } to apply it must also be flame proof, per the fire department and
building
: } codes.
: } (Recall the disasterous fire in the Rhode Island night club a
couple years
: } back).
: } Some years ago I found that the material required behind an XRD
system in
: } a small
: } room ran several hundred dollars for a half dozen panels.
However, I
: } found that I
: } could get a big improvement by placing a small number of them so
as to
: } block the
: } noise at the source, rather than covering the more distant wall
: } surfaces. Buy a
: } small number to try out, or experiment with packing foam. Have
an
: } assistant hold
: } them in different locations so as to block acoustic reflections.
You may
: } find that
: } suspending one from the ceiling in the plane of the instrument
helps alot with
: } noise at the position where the operator sits.
: }
: } John Twilley
: }
: } Gary Gaugler wrote:
: }
: } }
:
} ------------------------------------------------------------------
------------
: } } The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
: } } To Subscribe/Unsubscribe --
: } http://www.msa.microscopy.com/MicroscopyListserver
: } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
: } }
:
} ------------------------------------------------------------------
-------------
: } }
: } } I'm looking for sources of acoustic/anachoic
: } } blocks of foam to reduce SEM room interference.
: } }
: } } These are like standard anechoic chamber panels.
: } } I'm having trouble getting above 400KX without
: } } mechanical noise from the scroll pump that is
: } } located in a separate room. The acoustical
: } } isolation from one room to the other is not
: } } all that great, I suppose.
: } }
: } } Does anyone have experience with suppliers of
: } } these panels? I'd like to glue them to drywall.
: } }
: } } Supplier responses are welcomed as off-line
: } } messages.
: } }
: } } gary g.
:
:
:

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 01:14:19 2004

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chris, ron, and everyone else...

not wanting to sound paranoid, i think we have to consider chris's words
carefully.

while digital systems are good, after 35 years i cannot get the same
results from a digital image than i can from film, no matter how hard i
try. double exposures with polychromatic paper using different filters,
dodging, burning, combined, can contribute to excellent prints. perhaps
others can get the same results from digitized images, after 5 years, i
cannot.

we currently maintain two older Philips 201 and one cm10 microscopes.
they have 35mm cameras. Kodak has discontinued manufacture of Direct
Positive 5302, which we use for these instruments. the only sources of
which i now know for this film is the different suppliers. but how long
will their stocks last? what other 35mm format films are there that
have the same high resolution that is found with 5302?

all in all, the promise from Kodak is fine and dandy, but will that hold
when Kodak decides that the digital market has made it no longer viable
to support wet chemistry with specialized, high resolution films such as
we require.

of course, as far as i am concerned, i have 25 roles of 5302 in the
freezer, so i'm set until our microscopes die. but it is an ongoing
concern for the rest of you who were not ordering at the time that Kodak
made their decision and were not able to stock up.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 01:57:23 2004

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Dear Mr. Barclay,

we produce c-mount adapters which can be connected to the
eyepiece of a microscope. The eyepiece remains in place, you
put our eyepiece adapter on top of it. Our eyepiece adapter
has a lens built-in. This has the advantage of capturing most
of what you see through the eyepiece. You can easily remove
the adapter and look through the eyepiece again. We offer
different sizes of eyepiece adapters. We also manufacture on
demand.

If you would like to get more information please contact me.

mfg / regards

Anneliese Schmaus
Product Manager

klughammer gmbh
Strassbach 9
85229 Markt Indersdorf
Germany
Tel. +49 08136 6011
Fax +49 08136 7098
info-at-klughammer.de
www.klughammer.de

bwoM} ------------------------------------------------------------------------------
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bwoM} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jbarclay-at-southpointresources.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
bwoM} Sunday, September 5, 2004 at 08:40:08
bwoM} ---------------------------------------------------------------------------

bwoM} Email: jbarclay-at-southpointresources.com
bwoM} Name: Jim Barclay

bwoM} Organization: Calgary, Alberta

bwoM} Title-Subject: [Microscopy] [Filtered] Wild Heerbrug M5A microscope & camera attachment

bwoM} Question: I am trying to see if I can find some type of adaptor that will fit my older Wild Heerbrug M5A binocular microscope (15-20 years old?) and allow me to fit a digital camera to the
bwoM} scope. I would need some type of beam splitter that would continue to allow simultaneosu viewing of samples while also allowing occasional photo taking.

bwoM} I have already contacted and am waiting for reply from a Leica Microsystems representative which owns the Wild Leitz brand.

bwoM} Thank you for listening to a newbie to this board. Any suggestions welcome.

bwoM} JB.

bwoM} ---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 02:20:34 2004

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Chris Jeffree wrote the following:
======================================================================
In the same week there is news of significant change in two of the
companies that were
major players in silver image photography in the 20th century.
Ilford has shed almost half its staff preparative to sale of the
traditional photographic
business while it is still a going concern, allowing the company to
focus on its Swiss digital business.
http://www.channel4.com/news/news_story.jsp?storyId=156722
Agfa has shed its traditional photographic film and consumer imaging
business in a management buyout so that it can "focus on its core
growth markets of Graphic Systems and HealthCare, which are rapidly
going digital"
http://news.agfa.com/corporate/news.
nsf/news/F07C0210ECC86EA9C1256EF3004D27CE?opendocument

Events like these, and Kodak's announcement earlier this year that it would
cease the production of its poneering APS cameras (though not of films)
underline the fragility of the conventional photographic market in the face
of the growth of digital imaging.

Which begs the question "can we rely on the continued availability of
EM film", and if not, how long have we got
to plan for the conversion to digital?
============================================================================
Chris is correct in that there has been a real decline worldwide of emulsion
based photographic products.

But for the TEM film, the main people who are spreading the "fear" of a
"filmless day" soon to arrive are the ones who would benefit the most if one
did convert to all digital recording. There could be legitimate reasons to
do that, of course, but the inability to purchase high quality TEM film is
not going to be one of them at least not in the near and intermediate term
future. And besides, someone with a ten or more year old TEM probably is
not going to be too keen on making the large capital investment needed to
convert to digital anyhow, since the digital add-on would be worth far more
than the TEM onto which it is going.

When a large manufacturer decides to get out of a particular business, it is
not at all uncommon or unusual for them to find a smaller firm to continue
the manufacturing, marketing and distribution of the soon-to-be discontinued
product. This makes sense ethically as well since that way they don't leave
their existing customers "cut off at their knees". And what would seem like
"peanuts" to a large global manufacturer if not also a nuisance could be
seen as gigantic volume to a much smaller firm. This kind of licensing of
"mature" products being discontinued goes on all the time, including even
the marketplace for TEM film. For example, when Agfa " discontinued" the
Agfa Scientia brand of TEM film, they licensed a highly reputable German
photographic film manufacturing firm, MACO, to continue to manufacturer the
TEM films that users around the world had used for their work. And the MACO
TEM film is available from PLANO in Germany and from SPI Supplies everywhere
else. Everyone can rest well assured that MACO will continue to manufacture
TEM film well into the future, farther in fact than most would even want to
look.

More information and prices about the MACO film could be found at URL
http://www.2spi.com/catalog/photo/maco-TEM-film.html

Disclaimer: SPI Supplies is the worldwide distributor for the MACO TEM film
so we have an obviously vested interest in publicizing that fact.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 05:32:46 2004

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Chuck
Thanks for this clear statement of the position, which will surely
reassure many
TEM users like myself with a middle-aged instrument and little
prospect
of going digital at the moment. I will try to relax, but our
audio-visual services have
just refreshed my paranoia by announcing that they are no longer able
to source 35mm
slide projectors and lenses suitable for use in lecture theatres. (Is
that really true??)
We are therefore being encouraged to reduce our dependence on slides.

Best wishes
Chris

Dr. Chris Jeffree

----- Original Message -----
} From: "Garber, Charles A." {cgarber-at-2spi.com}
To: "MICROSCOPY BB" {Microscopy-at-MSA.Microscopy.com}
Sent: Monday, September 06, 2004 9:45 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (stokes-at-saturn.med.nyu.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday, September 5, 2004 at 19:04:28
---------------------------------------------------------------------------

Email: stokes-at-saturn.med.nyu.edu
Name: David Stokes

Organization: NYU Skirball Inst

Title-Subject: [Microscopy] [Filtered] EM Core Director

Question: The Skirball Institute of New York University School of Medicine seeks an individual to set up and direct an imaging core facility with an initial emphasis on electron microscopy. The Skirball Insitute, located in midtown Manhattan, consists of 35 laboratories with a diverse array of biomedical research projects, which are described in detail at http://saturn.med.nyu.edu. Individual laboratories currently operate two electron microscopes and several confocal microscopes together with various ancillary equipment for specimen preparation and image analysis. To organize these into a shared facility, we seek an individual with extensive experience in conventional thin sectioning and immunolabeling of biological organisms. The ideal individual will also have management skills and an ambition to develop a comprehensive facility with additional staff offering a wide range of imaging services. Applicants should send their curriculum vitae along with the names and addresses of three references to: Dr. David Stokes at stokes-at-saturn.med.nyu.edu.

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From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 08:13:55 2004

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Can anyone recommend a good commercial photolab that is
also reasonably priced, for scanning hundreds of 35 mm
slides and saving on CDs?

Sandra K. Masur, PhD
Prof. Ophthalmology
MSSM
NYC

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 14:57:27 2004

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Hi People

We here at the end of the world have already suffered from this "problem" for the last 4 or 5 years where Kodak could not supply us with "EM" grade film.
We still use film for all the reasons already quoted; particularly that it still produces the best results compared to digital.

Four years ago we switched suppliers to Agfa and use a product called Copex Positive Pet 10.
Code number 2OYAT CNP3 NP EI
This is in the 35mm format

Good Luck
Raymond Bennett

Keith Williamson EM Unit
HortResearch
Private Bag 11030
Palmerston North
NEW ZEALAND

} } } Paul Hazelton {paul_hazelton-at-umanitoba.ca} 09/06/04 4:06:52 a.m. } } }

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chris, ron, and everyone else...

not wanting to sound paranoid, i think we have to consider chris's words
carefully.

while digital systems are good, after 35 years i cannot get the same
results from a digital image than i can from film, no matter how hard i
try. double exposures with polychromatic paper using different filters,
dodging, burning, combined, can contribute to excellent prints. perhaps
others can get the same results from digitized images, after 5 years, i
cannot.

we currently maintain two older Philips 201 and one cm10 microscopes.
they have 35mm cameras. Kodak has discontinued manufacture of Direct
Positive 5302, which we use for these instruments. the only sources of
which i now know for this film is the different suppliers. but how long
will their stocks last? what other 35mm format films are there that
have the same high resolution that is found with 5302?

all in all, the promise from Kodak is fine and dandy, but will that hold
when Kodak decides that the digital market has made it no longer viable
to support wet chemistry with specialized, high resolution films such as
we require.

of course, as far as i am concerned, i have 25 roles of 5302 in the
freezer, so i'm set until our microscopes die. but it is an ongoing
concern for the rest of you who were not ordering at the time that Kodak
made their decision and were not able to stock up.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926

______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 15:59:54 2004

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TEM Research Scientist

Industrial Research Limited is New Zealand's leading industrial scientific research organisation. Our role is to develop leading-edge technologies into practical business opportunities (http://www.irl.cri.nz).

IRL is strengthening its research efforts in the area of superconducting materials and is now seeking expressions of interest from suitably qualified scientists for a two year Post-doctoral position, at our Gracefield site (near Lower Hutt, Wellington). This is an opportunity to join an internationally renowned team working on both Government and industry funded projects in the area of superconducting technologies.

Key responsibilities, knowledge and qualifications:
* To undertake applied research and development projects in superconducting materials, including the preparation and analysis of samples of so-called "2nd generation" Yttrium Barium Copper OxideYBCO tapes
* PhD or equivalent in materials science or a related field with a focus on TEM work.
* Experience in preparing TEM samples of composite materials is highly desirable
* To develop and contribute to the development of new research areas
* To take an active role in the technology transfer process
* Experience with superconducting materials is desirable but not essential
*
*
* The successful applicant will possess:
* Strong aptitude for experimental research and development
* Demonstrated TEM experience
* Good planning, organisational and problem solving skills
* An ability to work independently and as part of a team
* Superior oral and written communication skills

An attractive remuneration package commensurate with qualifications and experience will be offered to the successful candidate as well as a wonderful opportunity to live in a vibrant capital city in a country renowned for its quality of life and outdoor activities.

'Expressions or Interest' are invited and should be forwarded by 17 September 2004 to: Jennie Scott, Industrial Research Limited, P O Box 31-310, Lower Hutt, Wellington,
Phone: (04) 931 3094, Fax: (04) 569 0019, E-mail: j.scott-at-irl.cri.nz {mailto:j.scott-at-irl.cri.nz} .

Industrial Research Limited is an Equal Opportunities Employer

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 16:26:07 2004

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chuck

i had been thinking of looking up agfa, then they went out of the
field. does MACO produce a 35mm film, and is it of similar grain?

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 20:12:30 2004

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-----Original Message-----
} From: Paul Hazelton [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Sunday, September 05, 2004 11:07 AM

double exposures with polychromatic paper using different filters,
dodging, burning, combined, can contribute to excellent prints.

---------------------------------------

I don't mean to sound sarcastic, and I know it's going to, so please
understand that's not how I mean it.

How is the above different from manipulating a digital image?

I guess there are two parts to the question. First, is this any less of
a manipulation (and hence potential for inaccurate or inappropriate
artifacts) than using digital techniques to improve the appearance of an
image? I mention this in the context of other discussions on digital
image integrity.

Second, is the specific problem you're describing a limit of digital
technology, or a limit of the skills and resources available? It is
certainly a different set of skills to work in a "wet" darkroom than
that used in the "digital" darkroom. (Having worked some in both, I too
found the digital harder. None the less, I can see where given the
right set of tools and skills, the "useable quality" of digital and film
could be equal.)

John R.

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 21:41:28 2004

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Raymond Bennett wrote:
===============================================
We here at the end of the world have already suffered from this "problem"
for the last 4 or 5 years where Kodak could not supply us with "EM" grade
film.
We still use film for all the reasons already quoted; particularly that it
still produces the best results compared to digital.
===============================================
I am sympathetic to your problem but it is a different kind of problem, not
to be confused with the issue already being discussed on the listserver.
Your problem is a distribution problem and is the result of the way Kodak
(and other manufacturers) distribute(s) their films and other photographic
products. Now I am not the last word on this but I am sure someone from
Kodak would correct me quite quickly should I be wrong.

My perception is that Kodak establishes an exclusive distributorship,
sometimes their own subsidiary, to distribute film in a specific market,
such as NZ. But it is up to that particular distributor to decide what they
will either a) keep in stock or b) "handle" even on special order.
Unfortunately, too many such distributors decide that because the volume is
so low, and perhaps they don't want to end up with stale-dated film, they
just don't want to be bothered with the handling of such a specialty item
like the EM films so they tell their customers it is "not available" but of
course, they are really saying it is not available from them, even thought
it certainly could be avalable generally, such as in the USA or other
countries.

That is why so many end users in New Zealand purchase their Kodak EM film
from those firms already providing EM consumables, such as SPI Supplies, Ted
Pella, or Ladd (to name a few) in the USA. By ordering this way, you can
combine all your other needs for TEM supplies and consumables and the end
result is that the incremental shipping costs associated with the film can
become almost negligible if not zero. This problem is far from being
limited to NZ and in fact gets repeated in many other countries and markets
around the world.

With regard to getting superior results with film vs.digital, I hear this
all the time, but some would say it would depend on the quality of the
digital system you are using to make the comparison. Could you comment on
what digital system you used to make your comparison? Those of us who have
not converted yet could find your answer very interesting.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 22:09:56 2004

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Paul R. Hazelton wrote:
============================================================================
==
i had been thinking of looking up agfa, then they went out of the field.
does MACO produce a 35mm film, and is it of similar grain?
============================================================================
===
Was this a 35 mm film that actually went into the vacuum of the TEM or are
you talking about using it with a camera that photographed the screen
through a viewing glass from outside the vacuum? Do you have a former Agfa
product number?

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 6 23:01:14 2004

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john

certainly your comments are taken well. probably because i agree, and
that the whole intent of what i said is that i can manipulate the images
much better with wet chemistry after 35 years than i can after 4-5 years
digital. i know someone in the advertising business who tells me
digital is great, he just hires someone to do the digital work. i
cannot do that.

paul

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 02:28:03 2004

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Kodak is not the only maker of film on earth and even if 5302 is the
best film for the job it is not the only film that will work. Any
film can be a direct positive film with the right chemistry. Unless
there is some reason it needs to insensitive to red light there are
any number of films that with the right developers will work. If you
limit it to ortho film the field is a bit limited but there are
still a few others.

Gordon

} From: "Paul Hazelton" {paul_hazelton-at-umanitoba.ca}

:
: chris, ron, and everyone else...
:
: not wanting to sound paranoid, i think we have to consider chris's
words
: carefully.
:
: while digital systems are good, after 35 years i cannot get the
same
: results from a digital image than i can from film, no matter how
hard i
: try. double exposures with polychromatic paper using different
filters,
: dodging, burning, combined, can contribute to excellent prints.
perhaps
: others can get the same results from digitized images, after 5
years, i
: cannot.
:
: we currently maintain two older Philips 201 and one cm10
microscopes.
: they have 35mm cameras. Kodak has discontinued manufacture of
Direct
: Positive 5302, which we use for these instruments. the only
sources of
: which i now know for this film is the different suppliers. but
how long
: will their stocks last? what other 35mm format films are there
that
: have the same high resolution that is found with 5302?
:
: all in all, the promise from Kodak is fine and dandy, but will
that hold
: when Kodak decides that the digital market has made it no longer
viable
: to support wet chemistry with specialized, high resolution films
such as
: we require.
:
: of course, as far as i am concerned, i have 25 roles of 5302 in
the
: freezer, so i'm set until our microscopes die. but it is an
ongoing
: concern for the rest of you who were not ordering at the time that
Kodak
: made their decision and were not able to stock up.
:
: paul
:
: Paul R. Hazelton, PhD
: Electron Microscope Unit
: University of Manitoba
: Department of Medical Microbiology
: 531 Basic Medical Sciences Building
: 730 William Avenue
: Winnipeg, Manitoba, Canada, R3E 0W3
: e-mail: paul_hazelton-at-umanitoba.ca
: Phone:204-789-3313
: Pager:204-931-954
: Cell:204-781-1502
: Fax:204-789-3926
:
:
:
:
:
:

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 07:16:40 2004

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Unless the eye alone is used to view a scene (and almost every human eye is
different) some form of manipulation has occurred. The minute that an image
detector other than the eye is used, its response characteristics define what can
be seen or not seen. By definition, every mode of imaging other than that of
visible light has been through the selective filtration of some device. As soon as
the interpretive and adaptive mechanisms of the brain are divorced from the
immediate act of seeing a scene in context, in visible light, a greatly diminished
amount of information is available.

For conventional film photography every image has been manipulated from the moment
that the decision was made to record it. The choice of one film over another is a
choice to use a certain set of contrast and density limits over some other. This
is what professionals do - they make informed decisions about work that they are
uniquely trained to do. Anyone who is not prepared to take that responsibility for
the product of their work probably should stick to an arbitrary formula since they
apparently are no more qualified to make those judgments than those to whom any
exercise of professional judgment somehow looks suspicious.

John Twilley

Chiphead wrote:

} How is the above different from manipulating a digital image?
} is this any less of
} a manipulation (and hence potential for inaccurate or inappropriate
} artifacts) than using digital techniques to improve the appearance of an
} image?

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 08:05:29 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tnicklee-at-uhnresearch.ca) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 6, 2004 at 13:33:11
---------------------------------------------------------------------------

Email: tnicklee-at-uhnresearch.ca
Name: Trudey

Organization: Ontario Cancer Institute

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Has any one performed a dual immunohistochemistry staining, then removed, destained or stripped the antibodies and performed either a second series of immunofluoresecnce or immunohistochemistry? I have heard this discussed at conferences, but can not find a recent reference.
We initially are staining for HIF, CA9 and EF5 in fluorescence, which are working. We are then interested in restaining for CD34 (Nova Red in immunohistochemistry) which is a very robust antibody. CD34 should be in regions where HIF CA9 and EF5 are not, but for some reason it is not staining.
Thanks for any suggestions.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 08:05:11 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sarbu-at-mf.mpg.de) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 6, 2004 at 11:52:53
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Email: sarbu-at-mf.mpg.de
Name: Corneliu Sarbu

Organization: National Institute for Materials Research, Bucharest

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear colleagues,
does anybody know of a software able to assist in the geometrical drawing of the relationship between the Buergers vector, dislocation line direction, diffraction vector and perhaps electron beam direction (all expressed in a crystallographical way) in in a given material (of cubic symmetry) whose crystallographical constants are known ? The task can be fulfilled by hand drawing, in an approximate way, but I would be pleased to do it in authomatically, as I have to cope with a large variety of such configurations.
A software able to display dinamically the variation of all the above mentioned parameters would be great.

Thank you for your attention.

Dr. Corneliu Sarbu

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From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 08:06:36 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, September 7, 2004 at 06:04:51
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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I processed quite a few blocks of tissue in Spurr last week.
Some of the tissue was actually cell pellets embedded in conical
capsules. These blocks and some others in regular capsules did not
polymerize in the 2 days as I expected. I left them in the oven
over the Holiday, but they are still soft. I may have made the
Spurr wrong. Does anyone know if these will eventually cure
or can I dig out the tissue, put it into a Spurr/Propylene oxide
mixture and try to re-embed it? Of course these were the most
important blocks in all that I embedded!
Thanks.
Stacey Andringa

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From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 08:07:22 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (walter.bobrowski-at-pfizer.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, September 7, 2004 at 07:16:18
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Email: walter.bobrowski-at-pfizer.com
Name: Walt Bobrowski

Organization: Pfizer Global R&D

Title-Subject: [Microscopy] [Filtered] RE: DMP-30 vs. BDMA

Question: Thanks all for the wonderful advice. Yes, old habits die hard! Caroline, I've "always" used the Mollenhauer recipe as I liked it's cutting properties (it felt 'just right'). However, I'm attempting to optimize our 13-year old Lynx el automated processor and moving toward a less-viscous epoxy formulation (Luft with BDMA) for our routine embedding. Yes, I know that Spurr's is even less viscous, but didn't know they had re-formulated the components to be less toxic. I'll have to look into that.

Best regards,

Walter F. Bobrowski
Investigative Pathology
Safety Sciences
Pfizer Global Research & Development
Ann Arbor, MI 48105

TEL: 734-622-7814
FAX: 734-622-3478
Mobile: 734-646-0502

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From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 08:36:41 2004

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There are sound-deadening metal channels that are attached to the wall
studs, then drywall attached to them. A drywall supply firm could supply
them. These, and a second layer of 'rock' over the old one could obviate
tearing out the wall. With the enduring popularity of 'bass you can feel',
in popular 'music', this should be an ongoing concern. :0)

Paul Grover

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Saturday, September 04, 2004 12:01 PM
To: John Twilley
Cc: MSA listserver

Bill Robertson in his reply posting pointed out
a very good point about wall mass. My initial
thoughts were to put the acoustic tiles in the SEM room.
But the noise is more likely coming from the adjacent
room where the scroll pump and specimen interchange
pump are located. The specimen interchange pump
is not at issue. The scroll pump is an Edwards XDS 10.
Based on limited experience, it does not sound like
it is running as it should. Vacuum is fine but the
sound is odd. It makes a mechanical knocking noise.
I'm told that either these pumps work almost forever
when new or die quickly. Sounds like I have the latter.

In either case, wall mass has a high probability
of attention. The intervening wall is standard
drywall with 2x4 studs. There is no insulation
in the wall. I'm thinking of having liquid foam
insulation put in the wall cells and seeing if that
helps. In the mean time, the plinth anti-vibration
system is not exactly right and will get adjusted
shortly. then, based on how that turns out, acoustic
tiles in the pump room may be a good solution. If that
does not help much, then foam insulation.

The chiller is in the same room as the SEM but turning
it off makes no change in image noise. So the noise
is external.

Thanks for the replies. Will work on this in the
next couple of weeks.

gary g.

At 08:38 AM 9/4/2004, you wrote:
} Gary,
}
} They can be pricey. One reason is that most jurisdictions require that
} insulation
} applied to an open wall surface be flame proof. Not only that, the
} adhesive used
} to apply it must also be flame proof, per the fire department and building
} codes.
} (Recall the disasterous fire in the Rhode Island night club a couple years
} back).
} Some years ago I found that the material required behind an XRD system in
} a small
} room ran several hundred dollars for a half dozen panels. However, I
} found that I
} could get a big improvement by placing a small number of them so as to
} block the
} noise at the source, rather than covering the more distant wall
} surfaces. Buy a
} small number to try out, or experiment with packing foam. Have an
} assistant hold
} them in different locations so as to block acoustic reflections. You may
} find that
} suspending one from the ceiling in the plane of the instrument helps alot
with
} noise at the position where the operator sits.
}
} John Twilley
}
} Gary Gaugler wrote:
}
} }
}
----------------------------------------------------------------------------
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} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
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} }
} } I'm looking for sources of acoustic/anachoic
} } blocks of foam to reduce SEM room interference.
} }
} } These are like standard anechoic chamber panels.
} } I'm having trouble getting above 400KX without
} } mechanical noise from the scroll pump that is
} } located in a separate room. The acoustical
} } isolation from one room to the other is not
} } all that great, I suppose.
} }
} } Does anyone have experience with suppliers of
} } these panels? I'd like to glue them to drywall.
} }
} } Supplier responses are welcomed as off-line
} } messages.
} }
} } gary g.

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 09:23:18 2004

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If you can see the noise as discrete horizontal sinusoids, it is
probably low frequency. If you increase your scan speed and the
spacing widens proportionately, then you are looking at the true
frequency (not an 'aliasing' effect). Also, when aliasing is
occurring, the disturbance will often appear to be propagating at an
angle, rather than purely horizontal.

A simple way to estimate low frequencies is to employ something that
generates a strong 60Hz magnetic field as a reference (I have often used
a hand-held magnetic tape eraser). Set up with a relatively high scan
speed so that your noise appears as nice sinusoidal disturbances. Count
how many cusps you get in a convenient vertical distance. Then turn on
the magnetic field generator and do the same for its disturbance.
Calculate the ratio vs 60 Hz and you have a crude frequency estimate.

However, if you are seeing discrete sinusoidal disturbances and
especially since this is coming from a piece of equipment in a
neighboring room that is described as making a "knocking" sound,
relatively low frequencies sound like a good bet.

Fred Schamber
ASPEX, LLC

Gary Gaugler wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} I don't have any direct way of knowing whether the
} noise is high or low frequency. It only shows up
} above 60KX as sinusoids on the edges of specimen
} details.
}
} I can probably use FFT to compute the frequency.
} It is consistent.
}
} gary g.
}
}
} At 03:34 PM 9/5/2004, you wrote:
}
}
}
} } On Sep 3, 2004, at 6:30 PM, Gary Gaugler wrote:
} }
} } } I'm looking for sources of acoustic/anachoic
} } } blocks of foam to reduce SEM room interference.
} } }
} } } These are like standard anechoic chamber panels.
} } } I'm having trouble getting above 400KX without
} } } mechanical noise from the scroll pump that is
} } } located in a separate room. The acoustical
} } } isolation from one room to the other is not
} } } all that great, I suppose.
} } }
} } } Does anyone have experience with suppliers of
} } } these panels? I'd like to glue them to drywall.
} } }
} } } Supplier responses are welcomed as off-line
} } } messages.
} }
} } Dear Gary,
} } Fred has it right about the difference between low and high
} } frequencies. The depth of the invaginations in anechoic panels has
} } to be 1/4 of the wavelength of the sound in order to be effective, so
} } for low frequencies, the panels could take up the entire room,
} } leaving no space for the equipment.
} } Yours,
} } Bill Tivol, PhD
} } EM Scientist and Manager
} } Cryo-Electron Microscopy Facility
} } Broad Center, Mail Code 114-96
} } California Institute of Technology
} } Pasadena CA 91125
} } (626) 395-8833
} } tivol-at-caltech.edu
} }
} }
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 09:38:34 2004

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Any professional photo lab could do this for you. Since you are in
New York City finding a pro lab should be easy. However, high quality
film scanners (from Nikon, Minolta, etc). that do 35 mm slides are
relatively cheap these days, you might look into buying one and scanning
the slides yourself.

Geoff

Sandra Masur wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 10:44:17 2004

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On Sep 6, 2004, at 6:35 AM, Sandra Masur wrote:

} Can anyone recommend a good commercial photolab that is
} also reasonably priced, for scanning hundreds of 35 mm
} slides and saving on CDs?
}
Dear Sandra,
I have used Dale Labs in Florida for many years for my personal
photography, and one of the services they offer is to scan 35 mm slides
onto CDs. Their phone number is (800) 327-1776. I have no affiliation
with them except as a long-time satisfied customer.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 10:50:14 2004

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Hi,

Suggest that you contact the Royal Microscopical Society in Oxford, UK. They have a wide range of courses.

Here in the US, John Russ' course at North Carolina State is one of the best respected courses.

Hope this was helpful,
Barbara Foster
Microscopy/Microscopy Education

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018

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Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

At 01:53 AM 9/2/2004, Gareth Morgan wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 11:05:12 2004

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Dear Gary,
I have had some success here isolating the noise from the pump by putting
the pump up on a sound-deadening material such as two-inch-thick packing
foam, with a piece of plywood on top. I now have all my rotary pumps up on
pads. There can be considerable noise transmission through the floor to your
SEM column.
Regards and good luck,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "John Twilley" {jtwilley-at-sprynet.com}
Cc: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
Sent: Saturday, September 04, 2004 10:00 AM

MACO have contacted me to draw my attention to their company, which
produces EM film, X-ray film and other specialist B&W films, printing
paper and chemicals. The company partners Oriental in Japan and Lucky
in China, both large producers of silver photographic emulsions and
coated papers for digital printers.

I had not been aware of the existence of this company until I started
this thread on continued availability of EM film, and I suspect that
is true of many other EM users on this list.

MACO's web site is at http://www.mahn.net

Prompted by Nestor, I have suggested to MACO that they should list
themselves as a company on the MSA Commercial Organisations site
http://www.amc.anl.gov/docs/nonanl/wwwform.html

This is not an attempt to promote MACO in particular, and I have no
financial interest whatever in this particular company, although I
will be trying out their EM film if I can obtain a sample of it here.
I merely wish to draw attention to the fact that, as Chuck has pointed
out, this is a new company already poised to take over film production
if the big companies pull out. It would be a service to us all if
other emerging companies producing EM films and related photographic
materials and equipment could also be encouraged to register with the
Commercial Organisations site.

Best wishes
Chris

Dr. Chris Jeffree
University of Edinburgh

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 12:36:21 2004

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I have inherited some aged vials of crystalline osmium. They are a tad
older than normal which is to say 1940! But they look quite normal and are
sealed in glass ampules just like a more modern vintage. I have offered 30
x 1 gm vials so it was hard to not accept the gift; this would be a
lifetime supply. I intend to try them out in an experiment tomorrow. If
anyone knows why this is doomed to failure, please let me know
asap. Otherwise I will let the list know how it works out. Tom

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 13:39:18 2004

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I need to do some embedding in Lowicryl HM20 and will be ordering new resin kit. I noticed EMS is now offering "MonoStep" - a pre-mixed variation of the normal kit that supposedly gets rid of all the manipulations. Does anybody have some experience with this resin? I am a bit cautious about possible partial polymerization of the pre-made mixture.

Thanks,

Michael

--
Michael Jarnik, Ph.D.
Electron Microscope Facility
Fox Chase Cancer Center
7701 Burholme Ave.
Philadelphia, PA 19111
Tel. 215-728-5675
Fax 215-728-2412

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 15:54:56 2004

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I think you have a winner!
Chris

} I have inherited some aged vials of crystalline osmium. They are a
tad
} older than normal which is to say 1940! But they look quite normal
and are
} sealed in glass ampules just like a more modern vintage. I have
offered 30
} x 1 gm vials so it was hard to not accept the gift; this would be a
} lifetime supply. I intend to try them out in an experiment
tomorrow. If
} anyone knows why this is doomed to failure, please let me know
} asap. Otherwise I will let the list know how it works out. Tom
}
}
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 16:38:43 2004

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I know nothing about "dual" immunostaining" but would think that any
"destaining", stripping etc will equally affect antibodies and antigens, so
I don't expect anything good from it. From another hand, formally speaking
you do not limited to only "double" staining in immunofluorescence. With
luck, you may use for instance mouse, rat, rabbit, chicken primary
antibodies and correspondent secondary with different
fluorochromes. Modern confocal microscopes permits to analyze the whole
spectrum of emission, so you could separate signals quite easily. I don't
know is my approach realistic or not. It would be nice to hear expert's
opinion. From biochemical point of view, you could "strip" antibodies by
low pH (=2) or a few moles of urea, but it would affect your antigen as
well (for bad or good). Sergey

At 08:30 AM 9/7/2004 -0500, you wrote:

} ------------------------------------------------------------------------------
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From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 16:50:20 2004

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I haven't found any difference. Tom

At 03:04 PM 09/07/04 -0400, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 7 17:42:14 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jcai-at-nanostellar.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, September 7, 2004 at 12:57:14
---------------------------------------------------------------------------

Email: jcai-at-nanostellar.com
Name: Juan

Organization: Nanostellar

Title-Subject: [Microscopy] [Filtered] selective etching method for alumina

Question: Hi all,

I am looking for a way to chemically etch gama-alumina powders, without etching Ag and Cu nano-pariticles mixed in them.

The weight percentage of Ag or Cu in Alumina is around 0.5-1%. We have tried HF, but it turned out Ag and Cu are also etched out.

Thanks in advance for your advice,

Juan

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 8 02:45:39 2004

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Hello,
Please, could anybody of Philips CM100 users send me a list of coil currents
(C1, C2, OBJ etc.)in LM and M Mode at 80kV for magnifications of 1.4kx, 10.5kx
and 34kx, respectively. We have some troubles with our EM and unfortunately we
do not have a record of these values.
Thanking you in advance.
Best regards from Prague
Oldrich

+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 8 03:17:40 2004

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Stacey

it's unlikely that the samples will polymerise much more after a couple of days. You don't say whether you use Spurr's regularly and this is a first batch to go wrong.

You can certainly try re-embedding but be prepared for a slightly poorer block but be aware of the points below in case it happens again.

Spurr's can poorly embed for a variety of reasons:
1. incomplete dehydration, incomplete removal of alcohol - although if you're using propylene oxide as well I wouldn't have thought so.
2. incomplete impregnation with resin because too little time, not enough stages (eg 50%, 75%, 2x100%), not enough agitation/rotation.
3. bad mix either due to wrong amounts or incomplete mix.
4. One of the components has deteriorated (S1 curing agent may have a shelf life of 6-12 months; I believe that the NSA anhydride hardener can go off especially if exposed to moisture over time)

Spurr's can be affected by moisture so if you chill or freeze it, allow plenty of time for it to reach room temperature and don't leave the lids of the components or mixture for too long.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
} From: Stacey.Andringa-at-uc.edu (by way of MicroscopyListserver)

Announcement and Call for Papers
Michigan Microscopy &Microanalysis Society Fall Meeting, 2004

Kellogg Center
East Lansing, MI
November 5, 2004

Abstract Deadline: October 1, 2004

The Fall Meeting of the Michigan Microscopy and Microanalysis Society will
be held on November 5th, 2004 at the Kellogg Center in East Lansing, MI. In
this one-day conference, there will be two sessions. One is a platform
session. This session will have approximately 8-10 speakers representing
industry, academia, and research laboratories. The other is a poster
session. Please encourage your colleagues who prefer to avoid a platform
presentation to submit abstracts for the poster presentations. Some
selected abstracts for the oral presentation can be transferred to the
poster session if too many abstracts come for the platform session. In
addition to the speakers, vendors will exhibit a wide range of products and
services of interest to the microscopy community. Presentations are being
solicited from researchers in the Physical and Biological Sciences,
including one vendor presentation and an invited speaker. Student
participation is particularly encouraged. Also, vendors are encouraged to
contact the below address to reserve space for product display.

Abstract Submission
Please submit a 300 to 350 word abstract by October 1st indicating which
session you prefer (poster or presentation)

Geoff Williams
MMM President
217 Brooks Hall
Biology Department
Central Michigan University
Mt. Pleasant, MI 48859
Ph 989 774 3576
Fax 989 774 3462
Email: ge.willi-at-cmich.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 8 14:13:31 2004

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I need a non standard band pass filter and grating for a B &L
monochromatic grating. for 500 nm to 1,000 nm. I know these are not
standard. The band pass filter can be wider and a water cell would
be actable. I would also like to find a hot or cold mirror that had
a 1,100 to 1,400 cut off. All the filters need to have pretty high
transmittance in the pass band.

Thanks
Gordon
Gordon Couger gcc-at-couger.com

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward any links or information you think might be useful to
others.
Microscope Manual at www.science-info.org
-----

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 8 16:13:41 2004

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That's interesting - what was it used for in 1940?

Lesley Weston.

on 07/09/2004 11:03 AM, Tom Phillips at phillipst-at-missouri.edu wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
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--} -
}
} I have inherited some aged vials of crystalline osmium. They are a tad
} older than normal which is to say 1940! But they look quite normal and are
} sealed in glass ampules just like a more modern vintage. I have offered 30
} x 1 gm vials so it was hard to not accept the gift; this would be a
} lifetime supply. I intend to try them out in an experiment tomorrow. If
} anyone knows why this is doomed to failure, please let me know
} asap. Otherwise I will let the list know how it works out. Tom
}
}
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 8 17:16:25 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oh, what a good question! I do think osmium was used pre-EM days for some
speciality stains (neuro?) but I was given at least 30 ampules so that
seems like a lot for someone doing a speciality stain. I don't know which
department this chemical stock came from. This came to me via the
Environmental Health and Safety people on campus who try to recycle
"unwanted" but unused chemicals (a great program you should all get your
schools to copy since it says lots of disposal costs and reduces the
researcher expenses). It may have been used in some chemical lab but once
again seems like a lot. These vials were packaged by Merck (stamped made
in Germany), sold by Arthur Thomas and each encased in a nice little wood
case. They have 8/13/40 or 1940 written on them. I will let the list know
how the expt comes out. Tom

At 02:39 PM 09/08/04 -0700, you wrote:
} That's interesting - what was it used for in 1940?
}
} Lesley Weston.
}
}
} on 07/09/2004 11:03 AM, Tom Phillips at phillipst-at-missouri.edu wrote:
}
} }
} }
} }
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} --} -
} }
} } I have inherited some aged vials of crystalline osmium. They are a tad
} } older than normal which is to say 1940! But they look quite normal and are
} } sealed in glass ampules just like a more modern vintage. I have offered 30
} } x 1 gm vials so it was hard to not accept the gift; this would be a
} } lifetime supply. I intend to try them out in an experiment tomorrow. If
} } anyone knows why this is doomed to failure, please let me know
} } asap. Otherwise I will let the list know how it works out. Tom
} }
} }
} }
} } Thomas E. Phillips, PhD
} } Professor of Biological Sciences
} } Director, Molecular Cytology Core
} } 3 Tucker Hall
} } University of Missouri
} } Columbia, MO 65211-7400
} }
} } 573-882-4712 (office)
} } 573-882-0123 (fax)
} } PhillipsT-at-missouri.edu
} }
} }
} }

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 8 18:13:38 2004

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Hi,

OsO4 is used as a histological stain for light microscopy, this use may
have preceded its use in Electron Microscopy.

Aaron Hicks
Electron Microscopy Preparation Technician

Comparative Physiology and Anatomy
Institute of Veterinary, Animal, and Biomedical Sciences
Massey University

PN-412
Private Bag 11 222
Palmerston North
New Zealand

Phone +64 06 350 4470

-----Original Message-----
} From: Lesley Weston [mailto:lesley-at-vancouverbc.net]
Sent: Thursday, 9 September 2004 9:39 a.m.
To: Tom Phillips; Microscopy-at-msa.microscopy.com

That's interesting - what was it used for in 1940?

Lesley Weston.

on 07/09/2004 11:03 AM, Tom Phillips at phillipst-at-missouri.edu wrote:

}
}
} ----------------------------------------------------------------------
} --------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
--} -
}
} I have inherited some aged vials of crystalline osmium. They are a
} tad older than normal which is to say 1940! But they look quite
} normal and are sealed in glass ampules just like a more modern
} vintage. I have offered 30 x 1 gm vials so it was hard to not accept
} the gift; this would be a lifetime supply. I intend to try them out
} in an experiment tomorrow. If anyone knows why this is doomed to
} failure, please let me know asap. Otherwise I will let the list know
} how it works out. Tom
}
}
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 8 18:50:27 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (wwiggins-at-carolinas.org) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 8, 2004 at 12:41:52
---------------------------------------------------------------------------

Email: wwiggins-at-carolinas.org
Name: Winston Wiggins

Organization: Carolinas HealthCare System

Title-Subject: [Microscopy] [Filtered] Ilford

Question: Does anyone have any details about Ilford going under? There was a blurb in the Guardian about it last month but I've heard nothing here in the States. Worried because I just got an Ilford 2150XL processor 2 years ago and hoped I could stave off digital equipment for a longer while.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 9 02:32:47 2004

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Winston
I posted a comment about this to the list a few days ago. See below
Some further links:
http://www.guardian.co.uk/arts/news/story/0,11711,1289459,00.html
http://www.manchesteronline.co.uk/business/general/s/128/128120_fears_for_700_jobs_as_ilford_faces_closure.html
By contrast, Ilford's web site suggests situation normal!
Hope this helps
Chris

Dr. Chris Jeffree
Inveresk Cottage
26, Carberry Road
Inveresk
Musselburgh
Midlothian
EH21 8PR
Tel: +44 131 665 6062
FAX +44 131 653 6248
Mobile 07710 585 401
----- Original Message -----
} From: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
To: {microscopy-at-msa.microscopy.com}
Sent: Friday, September 03, 2004 9:05 AM

Dear listserver readers,
I am in the lucky position of getting a second hand Micrion 2500
focused ion beam microscope. All the bits are in the room, installation
(via FEI) should start soon. However I have no manual, and no information
on how it was configured in its previous life. Are there any users of this
machine out there who can give me some advice on the 'standard'
configuration (particularly things like the gas supplies) - and what it is
like to use/maintain? It looks quite frightening to an old TEM/SEM user
like me, UHV stainless steel and a million wires and tubes, more like some
experimental surface science kit than a routine spec prep tool..

Many thanks indeed

Richard

_______________________________
Richard Beanland
Analytical Services
Bookham Technology plc
Caswell,
Towcester,
Northamptonshire NN12 8EQ
UK
Tel: +44 (0) 1327 356362
Fax: +44 (0) 1327 356775
http://www.bookham.com

=======================================================================
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No part of this message can be considered a request for goods or
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From MicroscopyL-request-at-ns.microscopy.com Thu Sep 9 07:42:15 2004

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OsO4 is a classic stain for the basal bodies of ciliates. It's
important in ciliate systematics and morphology, as it reveals the
ciliary patterns at the light microscope level.
But.
It was usually applied to a dish of critters while watching through a
microscope.
Think Van Gogh's "Starry Night" ...

Phil

} That's interesting - what was it used for in 1940?
}
} Lesley Weston.
}
}
} on 07/09/2004 11:03 AM, Tom Phillips at phillipst-at-missouri.edu wrote:
}
} }
} }
} }
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} --} -
} }
} } I have inherited some aged vials of crystalline osmium. They are a tad
} } older than normal which is to say 1940! But they look quite normal and are
} } sealed in glass ampules just like a more modern vintage. I have offered 30
} } x 1 gm vials so it was hard to not accept the gift; this would be a
} } lifetime supply. I intend to try them out in an experiment tomorrow. If
} } anyone knows why this is doomed to failure, please let me know
} } asap. Otherwise I will let the list know how it works out. Tom
} }
} }
} }
} } Thomas E. Phillips, PhD
} } Professor of Biological Sciences
} } Director, Molecular Cytology Core
} } 3 Tucker Hall
} } University of Missouri
} } Columbia, MO 65211-7400
} }
} } 573-882-4712 (office)
} } 573-882-0123 (fax)
} } PhillipsT-at-missouri.edu
} }
} }
} }

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 9 08:00:08 2004

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Perhaps for Mann's osmium-sublimate fixative, Flemming's fixative or for
lipid staining.

Lesley Weston wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 9 09:55:13 2004

Contents Retrieved from Microscopy Listserver Archives
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Dear Richard,

Congratulations on your new 2500! It is a great tool,
but you are right, it is not really designed to be
easy service-able.

The "standard" configuration of gas supply boxes would
depend on what are you planning to do with the tool.
If you could please describe your intended
applications in some detail (materials you plan to
deal with and a type of work you intend to do), I
would be more then glad to help you with selection of
he gas configuration. There are also a lot of routine
service and periodic maintenance that you can do on
your own with some skill and perhaps a bit of
training.

If it is a standard, single-column 2500, then there is
no UHV involved, so you would not have to deal with
bake-outs.

Please contact me with your application details and if
(or rather when) you will have more questions
regarding the tool.

Best Regards,
Valery Ray
Particle Beam Systems
& Technology
www.partbeamsystech.com

--- Richard Beanland {richard.beanland-at-bookham.com}
wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Dear listserver readers,
} I am in the lucky position of getting a second hand
} Micrion 2500
} focused ion beam microscope. All the bits are in
} the room, installation
} (via FEI) should start soon. However I have no
} manual, and no information
} on how it was configured in its previous life. Are
} there any users of this
} machine out there who can give me some advice on the
} 'standard'
} configuration (particularly things like the gas
} supplies) - and what it is
} like to use/maintain? It looks quite frightening to
} an old TEM/SEM user
} like me, UHV stainless steel and a million wires and
} tubes, more like some
} experimental surface science kit than a routine spec
} prep tool..
}
} Many thanks indeed
}
} Richard
}
} _______________________________
} Richard Beanland
} Analytical Services
} Bookham Technology plc
} Caswell,
} Towcester,
} Northamptonshire NN12 8EQ
} UK
} Tel: +44 (0) 1327 356362
} Fax: +44 (0) 1327 356775
} http://www.bookham.com
}
}
}
=======================================================================
} This e-mail is intended for the person it is
} addressed to only. The
} information contained in it may be confidential
} and/or protected by
} law. If you are not the intended recipient of this
} message, you must
} not make any use of this information, or copy or
} show it to any
} person. Please contact us immediately to tell us
} that you have
} received this e-mail, and return the original to us.
} Any use,
} forwarding, printing or copying of this message is
} strictly prohibited.
} No part of this message can be considered a request
} for goods or
} services.
}
=======================================================================
}
}

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 9 11:10:22 2004

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers:
We have an SIS Megaview II mounted on the 35mm port of our Hitachi
H-7000. This interferes with the proper operation of the left specimen
traverse control. If there is anyone out there who has managed to
customize the system so that it operates properly, I would appreciate
hearing from them.
Thanks much
Greg

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 9 16:30:00 2004

Contents Retrieved from Microscopy Listserver Archives
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Hello Greg,

We have at least one other installation on an H-7000, where a pulley system
was used to "redirect" the control rod. I will send you a document directly
that shows the modifications. If you'd like I can get more information for
you (parts, prices, etc.)

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: Greg Erdos [mailto:gwe-at-biotech.ufl.edu]
Sent: Thursday, September 09, 2004 10:35
To: Microscopy-at-sparc5.microscopy.com

Dear Listers:
We have an SIS Megaview II mounted on the 35mm port of our Hitachi
H-7000. This interferes with the proper operation of the left specimen
traverse control. If there is anyone out there who has managed to
customize the system so that it operates properly, I would appreciate
hearing from them.
Thanks much
Greg

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 9 21:54:27 2004

Contents Retrieved from Microscopy Listserver Archives
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Greg,

The easy way (perhaps the easiest way) is to use flexible shaft. We use flexible shafts from Small Parts Inc. (Miami Lakes, FL),
www.smallparts.com or 800-220-4242 . Choose panel type, with 1/4" diameter bore at one end and 1/4" diameter shaft at the other end.
Then you can simply remove existing left specimen translation knob, attach bore end of the flexible shaft to the left control of the
specimen translation control axis, and attach removed knob to the rod end of the flexible shaft. You need also a small (rectangular)
piece of aluminum or whatever suitable material to hold the outer end of the shaft at the new location. External end of the flexible
shaft attaches to a panel (aluminum piece) in the same way as regular potentiometer or a toggle switch. Flexible shafts 6" and 12"
long are standard. Several can be joined for greater length. Part numbers Y-FDCP-4/6 and Y-FDCP-4/12 respectively, cost $19 and $23.

Please see one such camera installation on Hitachi HF-2000 TEM on our web site at http://www.sia-cam.com/2/photo2.html ;
http://www.sia-cam.com/2/photo3.html ; http://www.sia-cam.com/2/photo4.html . Both knobs were moved there. It took us 1 hour to
complete this modification from scratch.

Do not use flex. shaft much longer than needed, in order to avoid excessive free-play.

Rigid U-joint shafts can be used too, but modification becomes much more complex, since bearing support will be required on each
side of a U-joint.

You are welcome to contact me for further details.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
(678)467-0012 mobile
www.sia-cam.com

This message is made of 100% recycled electrons.

This address can not receive messages larger than 15 kb without prior
notification.

----- Original Message -----
} From: "Greg Erdos" {gwe-at-biotech.ufl.edu}
To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com}
Sent: Thursday, September 09, 2004 12:35 PM

Anyone know of a company that specializes in blue filters for microscopy.
We are looking for various sizes and need quite a few for student scopes,
some that are not standard sizes. Obviously the dealers have some sizes,
but I am really looking for a company that carries a large range of sizes
and types.

Thanks for the help!
David Burton
Optical Specialist
University of Washington

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 10 05:10:01 2004

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
Would anyone be kind enough to comment on the relative merits of
lens-coupled and fibre-optic-coupled CCD cameras for use at the wide
angle (35 mm) port? I am currently investigating the best choice for
the recording of diffraction patterns on a FEI Tecnai.

Thanks

--
Ian MacLaren
Department of Physics and Astronomy
University of Glasgow
Glasgow G12 8QQ
Scotland
http://www.ssp.gla.ac.uk/

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 10 09:05:26 2004

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Dear All:

We have a Hitachi H-7500 TEM, and want to have objective aperture
strips with four 20 micron apertures made for our scope.�Can anyone
recommend a company?�Thank you.

Hong
Emory EM

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 10 12:23:58 2004

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Dear Hong:

Yes, Ladd Research can provide a customized aperture for your scope. I
believe we are the only supplier of customized apertures in the U.S., but we
can make them available through other EM suppliers if you choose.

Deb Sicard

*Disclaimer: Ladd Research is a dealer of microscopy supplies and
accessories

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: ladres-at-att.net

----- Original Message -----
} From: "Hong Yi" {hyi-at-emory.edu}
To: {microscopy-at-ns.microscopy.com}
Sent: Friday, September 10, 2004 10:30 AM

Dear All:

We have a Hitachi H-7500 TEM, and want to have objective aperture
strips with four 20 micron apertures made for our scope. Can anyone
recommend a company? Thank you.

Hong
Emory EM

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 10 13:52:14 2004

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In a message dated 9/9/2004 10:07:31 PM US Mountain Standard Time,
dburton-at-nwlink.com writes:
Anyone know of a company that specializes in blue filters for microscopy.
We are looking for various sizes and need quite a few for student scopes,
some that are not standard sizes. Obviously the dealers have some sizes,
but I am really looking for a company that carries a large range of sizes
and types.
David,

The "default" size for such filters seems to be 24 mm or 1 inch, which is
probably what you'll find from most of the suppliers unless you want to get
plastic filter material in sheet form and make your own.

Try the following:

Edmund Optical at [www.edmundoptical.com]. Click on their Online Catalog,
then =} Optics =} Filters and Diffusers =} Color. You'll get several options,
such as plastic, glass, wratten filters, etc.

Chroma Technology at [www.chroma.com], Toll Free: 1-800-824-7662. They're a
specialty filter manufacturer, but I've used them before for simple blue scope
filters. They can cut and mount the filters in any size you want. Paul
Millman is the person to talk to.

Good luck, hope this helps.

Cheers,

Bob Chiovetti
RMC Products / Boeckeler Instruments, Inc.

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 10 14:58:15 2004

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Hi Fellow Microscopists,

I am immunolabeling cryothin sections of tissue that has been lightly fixed in 2 or 4%
paraformaldehyde with 0.1% glut, then cryoprotected, frozen and thin sectioned.
Does anyone have antigen unmasking techniques that you are very pleased with for cryothins? If
so I would be so happy to hear about them.
I have tried 0.005% trypsin, hot citrate buffer, hot tris 9.0 and 0.3% SDS and have had no effect
on the antigens that I am trying to unmask. Morphology, however, has suffered!
Thank you for any info advance.

Robert Underwood
Research Scientist
University of Washington

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 10 18:20:54 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (konishi-at-geofourpeaks.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, September 10, 2004 at 14:57:52
---------------------------------------------------------------------------

Email: konishi-at-geofourpeaks.com
Name: Hiromi Konishi

Organization: Indiana University

Education: Graduate College

Location: Bloomington, Indiana

Question: } I am asking about how to estimate the number of atoms at the surface of nano particles.
}
} You can roughly estimate the number using volumes of sphere and unit cell. If you assume that �surface� is the region between two spheres that have a slightly different diameter, you can calculate the volume of �surface� and estimate the number of atoms in the �surface�. However, it is a very rough estimation, and it is not clear how to chose the depth to define the surface.
}
} I would like to know general method in calculation of the number of atoms on surface. Also, if there is a program for such calculation, please advise.
}
} Thank you,
} Hiromi Konishi
} Indiana University

PS. I am a member of the list, but currently I cannot post my message for unknow reason, so I used this form.

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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 10 20:11:56 2004

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Dear Colleagues:

I would like to transfer the license of MacTempas (HRTEM Image
Simulation Software package for Apple) with USB hardware key (fair
price!).
Interested please contact : benyam-at-recite.ca

Regards,
Benyam

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 11 16:10:56 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tauria-at-hotmail.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, September 11, 2004 at 12:06:51
---------------------------------------------------------------------------

Email: tauria-at-hotmail.com
Name: FRANCIS J. PRONESTI

Organization: WORLD ENERGY SERVICES

Education: Graduate College

Location: CASTELLANA GROTTE (BARI), ITALY

Question: I HAVE AN OLD UNITRON SERIES "N" METALURGICAL MICROSCOPE, PURCHASED RECENTLY ON EBAY. I DO OWE MANY OTHER MICROSCOPES, BUT I HAVE FALLEN IN LOVE WITH THIS BEAUTIFUL, OPTICALLY COMPLEX INSTRUMENT. THERE IS NOTHING INTUITIVE ABOUT ITS SET-UP AND USE, SO I WONDER IF ANYONE WOULD KNOW WHERE I CAN BUY A COPY OF THE OPERATING MANUAL FOR THIS SCOPE.
FRANCIS J. PRONESTI
MSME, MSEE, MEMBER IEEE.
PRESIDENT AND OWNER, WORLD ENERGY SERVICES LTD.

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From MicroscopyL-request-at-ns.microscopy.com Sat Sep 11 16:16:30 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dmk8533-at-louisiana.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 10, 2004 at 14:05:22
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Email: dmk8533-at-louisiana.edu
Name: David Krayesky

Organization: University of Louisiana

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I have a Olympus Vanox fluoresence microscope in my lab. I need a manual for the microscope to explain what all the different markings mean on the various filters, so I can figure out what the filters do. Does anyone know where I can get this information? I haven't had a lot of luck at Olympus's web site or the FSU microscopy Primer web site.

Thanks,

Dave

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From MicroscopyL-request-at-ns.microscopy.com Sat Sep 11 16:17:53 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (burnin1970-at-hotmail.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 10, 2004 at 23:17:54
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Email: burnin1970-at-hotmail.com
Name: Andrei Burnin

Organization: Dartmouth College

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Recently, we tried EDS analysis of non-carbon nanotubes (20-30 nm in diameter) in TEM microscope (Tecnai F20).
The signals from compositional elements are very tiny in comparison with the cupper and carbon signals, which makes perfect sense, because we used carbon coated grids.
However, in the literature such EDS spectra from a spot in one (not from a bunch), say, a ZnO nanotube exhibits signals comparable to those from Cu-C background. How it is possible, that people obtain so high intensity of compositional elements from a very small analyzed area?
What should also we do to improve our results of getting EDS from a single nanotube?
Is it necessary to use beryllium grids?
Thank you.

Andrei

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From MicroscopyL-request-at-ns.microscopy.com Sat Sep 11 16:19:05 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kssim-at-mmu.edu.my) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, September 11, 2004 at 04:49:50
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Email: kssim-at-mmu.edu.my
Name: kssim

Organization: mmu

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear all,

I need help from anyone who know the following question:-

Basically I are going to prove that SE3 (secondary electron generated by BSEs at the pole-piece and the chamber wall from returning to the specimen) does not contribute to SE yield. The pole-piece of the SEM is covered by a 2 by 2 inch aluminum (Al) plate that has been painted with carbon paint to absorb SE3s. I need to know the bethe range of K-O range of the target which is carbon on the Al. I could not find this data from 10kev to 30kev range. I believe that with the thickness of 10um is good enough, but not a good reference. Can anyone help me on this?

But expertise says that the SE yield of both AL and carbon are not so high - refer to DC Joy journal paper. So the true SE3 contribution can be much higher. This is one of the points that I need to prove that SE3 still can be ignored.

Thanks
Ks

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From MicroscopyL-request-at-ns.microscopy.com Sun Sep 12 09:55:36 2004

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There is a general statement at http://www.amtimaging.com/english_site/faq_e/faq_e.html#3, not specific to the 35 mm port. However, I too would like to learn more on this.

----
Divakar
------
Physical Metallurgy Section,
Indira Gandhi Centre for Atomic Research.
Kalpakkam, TN 603102, India

-----Original Message-----
} From: Ian MacLaren [SMTP:i.maclaren-at-physics.gla.ac.uk]
Sent: Monday, September 13, 2004 9:17 AM
To: Microscopy-at-MSA.Microscopy.Com

Dear all,
Would anyone be kind enough to comment on the relative merits of
lens-coupled and fibre-optic-coupled CCD cameras for use at the wide
angle (35 mm) port? I am currently investigating the best choice for
the recording of diffraction patterns on a FEI Tecnai.

Thanks

--
Ian MacLaren
Department of Physics and Astronomy
University of Glasgow
Glasgow G12 8QQ
Scotland
http://www.ssp.gla.ac.uk/

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 08:06:37 2004

Contents Retrieved from Microscopy Listserver Archives
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The job below is for a TEM specialist although you have to read well
through the job particulars
http://www.materials.ox.ac.uk/vacancies/Job-Begbroke.pdf to find that out.

----------------------------------
UNIVERSITY OF OXFORD
Begbroke Science Park
Business-Development Materials Analyst
Academic-Related (Research) Grade RSIa - Salary Range - �19,460 - �28,128

Applications are invited for the post of Business Development Materials
Analyst for a period of up to two years. The Business Development
Materials Analyst will be working for the Institute of Aerospace and
Automotive Studies, in collaboration with Faraday Advance and the Oxford
Materials Characterisation Service. He or she will be responsible for
customer-oriented provision of industrial and analytical materials
characterisation services, including provision of rapid high-quality
investigations and proposals for materials related problems, and will
work to broaden and strengthen the academic and commercial relationships
of the OMCS.

The successful candidate is likely to have a track record in materials
characterisation, particularly in operating electron microscopes and
interpreting the resultant data, an appreciation of business issues
including Intellectual Property Rights, budgeting, management and
marketing, and the ability to communicate effectively to a range of
audiences. An understanding of the Aerospace and Automotive sectors
would also be beneficial.

Further particulars of the post are available from Mr Michael Sloane,
Begbroke Directorate, Oxford University Begbroke Science Park, Sandy
Lane, Yarnton, Oxfordshrire, OX5 1PF. email:
michael.sloane-at-begbroke.ox.ac.uk.

Applications, including a covering letter indicating how the applicant
meets the requirements of the post together with a detailed curriculum
vitae, should reach Mr. Sloane at the above address, by no later than
September 30th, 2004. Please include full contact details for three
referees, of whom one must be an existing or recent employer. The post
can be discussed informally with Dr Alison Crossley,
alison.crossley-at-materials.ox.ac.uk, 01865 283726.

The University is an equal opportunities employer.

See also http://www.ox.ac.uk/jobs
--
Chris Salter,
Oxford Materials Characterisation Service,
&
Material Science-based Archaeology Group,
&
Electron Microscopy Research Support Group,
Oxford University Begbroke Science Park,
Sandy Lane, Yarnton, Oxford, OX5 1PF
Tel 01865 283722, EPMA 283741, Mobile 07776031608

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 09:14:18 2004

Contents Retrieved from Microscopy Listserver Archives
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I am looking for Windows software that allows me to see crystal structure with
a less expensive cost. I want to use the software for quick check of number of
atoms in a unit cell and distance between atoms, looking at crystal structure,
and making figures for publication. Is there any recommendation?

Thank you,

Hiromi Konishi
Indiana University

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 10:14:59 2004

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers,

I'm interested in hearing opinions (pro/con) from anyone that has
experience with the Aspex PSEM. Please call/e-mail me off
list if you wish. Thanks for your input and time.

Ed Dargelis
Engineering Systems, Inc.
3851 Exchange Ave.
Aurora, Il 60504
(630) 851-4566
evdargelis-at-esi-il.com

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 10:28:30 2004

Contents Retrieved from Microscopy Listserver Archives
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ATOMS is a very good software which I used to do all kinds of work you want to do. But it is a commercial software, I do not know its exact price.

You can go to IUCr's (International Union of Crystallography)Webster and check their software page, you will find a lot of free software.

Best regards,

Ying

-----Original Message-----
} From: hkonishi-at-indiana.edu [mailto:hkonishi-at-indiana.edu]
Sent: Monday, September 13, 2004 9:42 AM
To: Microscopy-at-MSA.Microscopy.Com

I am looking for Windows software that allows me to see crystal structure with
a less expensive cost. I want to use the software for quick check of number of
atoms in a unit cell and distance between atoms, looking at crystal structure,
and making figures for publication. Is there any recommendation?

Thank you,

Hiromi Konishi
Indiana University

****************************************************************************************

Note: The information contained in this message may be privileged and confidential and thus protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you.

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From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 10:34:54 2004

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Marcus, are you out there?
If anyone knows how to contact Marcus, please respond off line.
Thanks,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 11:24:25 2004

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Dear Pei & Jorgen,

A tool developed in '99, visit www.Evactron.com actively prevents sample
'image darkening effects' from polymerized hydrocarbon contamination. The
device is commercially available, improves DP & TMP system vacuum levels
safely without damage to SEM/FIB components and eliminates manual iso
swabbing cleanup.

XEI Scientific

-----Original Message-----
} From: j.bilde-at-risoe.dk [mailto:j.bilde-at-risoe.dk]
Sent: Thursday, September 02, 2004 12:16 AM
To: pzou-at-feico.com
Cc: microscopy-at-microscopy.com

Dear Pei Zou,

See section 9.10.6 in Goldstein et al.: Scanning electron microscopy and
X-ray analysis" Plenum Press 1992. It begins: "A sample subjected to
electron bombardment in a diffusion-pumped vacuum gradually becomes ccovered
with a contamination layer due to polymerization, under the action of the
beam, of organic matter adsorbed on the surface".

Ways to reduce the effect are: clean vacuum, clean sample, cold finger.

Best regards,
Jorgen.

{:::} --- {:::} --- {:::} --- {:::} --- {:::} --- {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:pzou-at-feico.com]
Sent: 2. september 2004 04:16
To: microscopy-at-microscopy.com

Dear Gary,

I use 4 " sorbothane " mounts (stock number: NT35-264).
Usually I use it for holography but it worked well for damping the rot.
pump vibrations.

---
Shock Absorbing Sorbothane�
{http://www.edmundoptics.com/onlinecatalog/displayproduct.cfm?productID=1806&search=1}
Easily Cut to Size � Ideal For Use In Labs and Assembly Shops Absorbs
up to 95.5% of impact energy, then reforms to its original shape.
Sorbothane� is a solid that behaves like a liquid by absorbing...

Sorbothane� Mounts
{http://www.edmundoptics.com/onlinecatalog/displayproduct.cfm?productID=1618&search=1}

Patented Sorbothane� polymer mount absorbs (57% absorption efficiency)
and dissipates energy (70% specific damping). Reduces vibration to other
components by isolating the vibrations within the materi...

Bases and Platforms
{http://www.edmundoptics.com/onlinecatalog/displayproduct.cfm?productID=1255&search=1}

Large Workstation Platform: Hard-coat black anodized aluminum, 13"W x
18"L x 1/2"T platform with sorbothane feet and 3/4" threaded post hole.
Weighs 12 lbs. Small Platform Base: Hard-coated black anod...
---

Best regards
Timo Junker

Gary Gaugler schrieb:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} I'm looking for sources of acoustic/anachoic
} blocks of foam to reduce SEM room interference.
}
} These are like standard anechoic chamber panels.
} I'm having trouble getting above 400KX without
} mechanical noise from the scroll pump that is
} located in a separate room. The acoustical
} isolation from one room to the other is not
} all that great, I suppose.
}
} Does anyone have experience with suppliers of
} these panels? I'd like to glue them to drywall.
}
} Supplier responses are welcomed as off-line
} messages.
}
} gary g.

--
Timo Junker Holografie
Lindenstr. 10
97297 Waldb�ttelbrunn
Tel: ++49 (0)931 4520655
Fax: ++49 (0)931 4520657
www.holografie.com

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 11:59:54 2004

Contents Retrieved from Microscopy Listserver Archives
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Francis:

I would suggest that you contact John Coyle at Unitron. You can reach
him by email at : johnc-at-unitronusa.com.

DISCLAIMER: South Bay Technology distributes Unitron Microscopes and,
therefore, has a vested interest in promoting their use.

Best regards-

David

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 12:56:44 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It seems that during the summer months, we have no end of troubles with our
plastic not infiltrating the tissue. I've always assumed that this was
because of the humidity rising quite a bit during the summer months, and the
poor air conditioning.

It comes out as sections falling apart when I try to flatten them in the
water boat, or breaking in the electron beam. We have always kept our epoxy
resin in the freezer in 30 ml plastic syringes, but it usually only gives us
a problem during the summer months. During the winter here, the air is
extremely dry.

We do 4 rinses in absolute alcohol, followed by 3 rinses in propylene oxide,
followed by a 50/50 mixture of epon/araldite mixed with propylene oxide over
night with the caps off, for the propylene oxide evaporating overnight and
slowly leaving the specimens in 100% plastic by morning. We polymerize at
70 deg C overnight, keeping a dessicant in our embedding oven to keep the
air as dry as possible too.

Are there any resins which might be used for electron microscopy which are
more tolerant of water, and which would give a good result with human tissue
in the electron microscope?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 13:13:39 2004

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Thanks everyone. I've found him.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 13:23:55 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think Mercury is a free viewer. Check out
http://www.ccdc.cam.ac.uk/products/csd_system/mercury/ It is decent and
works in windows.

-----Original Message-----
} From: hkonishi-at-indiana.edu [mailto:hkonishi-at-indiana.edu]
Sent: Monday, September 13, 2004 9:42 AM
To: Microscopy-at-MSA.Microscopy.Com

I am looking for Windows software that allows me to see crystal structure
with
a less expensive cost. I want to use the software for quick check of number
of
atoms in a unit cell and distance between atoms, looking at crystal
structure,
and making figures for publication. Is there any recommendation?

Thank you,

Hiromi Konishi
Indiana University

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 13:30:21 2004

Contents Retrieved from Microscopy Listserver Archives
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Dear Andrei,
The subject of EDS spectra in a TEM or STEM is complex and there are many
different configurations of EDS detector in the TEM column. Some of these
account for the differences in relative background and specific element
signal. If you have a horizontal-mount detector and then tilt the sample
towards the detector, this will pick up a lot of grid and carbon x-rays. I
have a TEM with a high-takeoff-angle detector (68 degrees) and that results
in a much lower Cu and C signal when looking at tiny particles on a
carbon-coated copper grid. A 30 mm2 detector will also pick up more counts
than a 10 mm2. The material in the specimen holder and the column above and
below the sample also affects the relative background of your spectrum. A
TEM really needs to be designed for EDS at the factory, with light-element
inserts to reduce the secondary x-ray radiation that generates much of your
background. The better spectra you are referring to may have come from a TEM
or STEM designed or modified to give the best EDS performance, perhaps at
the cost of less performance in other areas. I know my TEM was designed for
analytical work, at the sacrifice of ultimate resolution.
To increase your counts you can try lowering the accelerating voltage,
increasing the spot size, increasing the condenser aperture size and, of
course, increasing the time you count for. Make sure your objective aperture
is removed, the specimen holder is specified for EDS and your EDS detector
is properly aligned.
Good luck,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca

----- Original Message -----
} From: "by way of MicroscopyListserver" {burnin1970-at-hotmail.com}
To: {microscopy-at-microscopy.com}
Sent: Saturday, September 11, 2004 2:43 PM

Hi, David

There are several sources, depending on the optical requirements. Both Chroma and Omega Filters provide an extensive line of filters. If this are fairly simple scopes, you might also try looking at Edmund Scientific.

If you have trouble finding any of the above, please contact me off-line.

Hope this is helpful.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018

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Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.
At 11:20 PM 9/9/2004, David Burton wrote:

} ------------------------------------------------------------------------------
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From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 15:51:36 2004

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Gary;
Your procedure is similar to the one I use here in Houston where we have
very high humidity also. The difference is that my Epon/araldite: PO step
is for one hour followed by one hour in pure resin then embedded in
capsules in fresh resin. This sets at RT for 3-4 hours then in the oven
overnight at 80 degrees C.
I have been using this method for over 20 years with no problems.

Mannie Steglich
Tech Dir E M Lab
M D Anderson Cancer Center

Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}

09/13/2004 01:24 PM

To:
{Microscopy-at-msa.microscopy.com}
cc:

It seems that during the summer months, we have no end of troubles with
our
plastic not infiltrating the tissue. I've always assumed that this was
because of the humidity rising quite a bit during the summer months, and
the
poor air conditioning.

It comes out as sections falling apart when I try to flatten them in the
water boat, or breaking in the electron beam. We have always kept our
epoxy
resin in the freezer in 30 ml plastic syringes, but it usually only gives
us
a problem during the summer months. During the winter here, the air is
extremely dry.

We do 4 rinses in absolute alcohol, followed by 3 rinses in propylene
oxide,
followed by a 50/50 mixture of epon/araldite mixed with propylene oxide
over
night with the caps off, for the propylene oxide evaporating overnight and
slowly leaving the specimens in 100% plastic by morning. We polymerize at
70 deg C overnight, keeping a dessicant in our embedding oven to keep the
air as dry as possible too.

Are there any resins which might be used for electron microscopy which are
more tolerant of water, and which would give a good result with human
tissue
in the electron microscope?

This e-mail and/or any documents in this transmission is intended for the
address(s) only and may contain legally privileged or confidential
information. Any unauthorized use, disclosure, distribution, copying or
dissemination is strictly prohibited. If you receive this transmission in
error, please notify the sender immediately and return the original.

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 16:19:13 2004

Contents Retrieved from Microscopy Listserver Archives
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Hi, All-

I have two JB-4 microtomes that I would like to repair, even if only to
get one to work. Neither advance. One has electronic advance and the other
doesn't. I have experience in repairing older ultramicrotomes, but I'd
like to have a set of service instructions, a blueprint, or even a users'
manual in hand before tearing them apart!

Does anyone have these documents? More importantly, perhaps, advice?

Mahalo,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 17:29:13 2004

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Osmium was used in the old days for treatment of arthritis. It
appears to be making a comeback......

http://rheumatology.oupjournals.org/cgi/content/abstract/42/9/1036

}
} That's interesting - what was it used for in 1940?
}
} Lesley Weston.
}
} on 07/09/2004 11:03 AM, Tom Phillips at phillipst-at-missouri.edu wrote:
}
} }
} } I have inherited some aged vials of crystalline osmium. They are a tad
} } older than normal which is to say 1940! But they look quite normal and are
} } sealed in glass ampules just like a more modern vintage. I have offered 30
} } x 1 gm vials so it was hard to not accept the gift; this would be a
} } lifetime supply. I intend to try them out in an experiment tomorrow. If
} } anyone knows why this is doomed to failure, please let me know
} } asap. Otherwise I will let the list know how it works out. Tom
} }
} }
} }
} } Thomas E. Phillips, PhD

--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 18:23:35 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (adriana-at-cab.cnea.gov.ar) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 13, 2004 at 13:56:32
---------------------------------------------------------------------------

Email: adriana-at-cab.cnea.gov.ar
Name: Adriana Cond�

Organization: CONICET, Argentina.

Title-Subject: [Microscopy] [Filtered] MListserver: CCD for TEM in material science

Question:
Dear microscopists,

We are interested in buying a CCD camera for a Philips CM200 TEM with an ultratwin lens for HRTEM. The main use is for materials science.
We would like to hear comments on the performance of the KeenView CCD camera offered by SIS.
We are particularly concerned with the sensibility for HRTEM (for example typical exposure times) and with the performance of the 12 bit camera for diffraction pattern acquisition and for online astigmatism correction using FFT.

All comments will be appreciated.

Thank you for your help.

Adriana

----------------------------------------------------------
Adriana Cond�
CONICET Researcher
Metals Physics Group
Centro At�mico Bariloche
8400 San Carlos de Bariloche
ARGENTINA
e-mail: adriana-at-cab.cnea.gov.ar
Fax: +54 2944 445299
TE: +54 2944 445290
----------------------------------------------------------

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 13 19:30:57 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alfred Nobel used Osmium in the production of Explosives in the 1860's. He
is best known for discovering Nitroglycerin & Dynamite (TNT). Now of course
his last will & testament funds "The Nobel Prize".

Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com

----- Original Message -----
} From: {sbarlow-at-sunstroke.sdsu.edu}
To: {microscopy-at-msa.microscopy.com}
Sent: Monday, September 13, 2004 6:56 PM

}
} It seems that during the summer months, we have no end of
} troubles with our plastic not infiltrating the tissue. I've
} always assumed that this was because of the humidity rising
} quite a bit during the summer months, and the poor air conditioning.
}
} It comes out as sections falling apart when I try to flatten
} them in the water boat, or breaking in the electron beam. We
} have always kept our epoxy resin in the freezer in 30 ml
} plastic syringes, but it usually only gives us a problem
} during the summer months. During the winter here, the air is
} extremely dry.
}
} We do 4 rinses in absolute alcohol, followed by 3 rinses in
} propylene oxide, followed by a 50/50 mixture of epon/araldite
} mixed with propylene oxide over night with the caps off, for
} the propylene oxide evaporating overnight and slowly leaving
} the specimens in 100% plastic by morning. We polymerize at
} 70 deg C overnight, keeping a dessicant in our embedding oven
} to keep the air as dry as possible too.

Is dessicant effective at 70 deg C, or will it release
moisture accumulated at room temperatures?

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 14 08:15:50 2004

Contents Retrieved from Microscopy Listserver Archives
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I've been following this thread of conversation on old OsO4 with great interest.
I know that there are many (?) books, articles written on the history of
microscopes (esp. electron microscopes), but is there an article/book written on
such interesting facts i.e. OsO4 and othere EM fixatives, etc. I would love to
read more......

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

-----Original Message-----
} From: Sample Prep [mailto:sampleprep-at-earthlink.net]
Sent: Monday, September 13, 2004 8:56 PM
To: microscopy-at-msa.microscopy.com; sbarlow-at-sunstroke.sdsu.edu

Alfred Nobel used Osmium in the production of Explosives in the 1860's. He
is best known for discovering Nitroglycerin & Dynamite (TNT). Now of course
his last will & testament funds "The Nobel Prize".

Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com

----- Original Message -----
} From: {sbarlow-at-sunstroke.sdsu.edu}
To: {microscopy-at-msa.microscopy.com}
Sent: Monday, September 13, 2004 6:56 PM

Hi Tina,

We manufacture the JB-4 and JB-4A here at EBS. It is our policy to
provide user manuals and telephone support for all of our instruments at
no charge. Please send us your contact information and we'll mail a
manual right out to you. Beyond that, we can also provide you with
factory parts and professional repair services, in the event that you
should you need either.

Sincerely,

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
800 992-9037
mnesta-at-ebsciences.com
www.ebsciences.com
"Adding Brilliance to Your Vision"

Tina Carvalho wrote:
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi, All-
}
} I have two JB-4 microtomes that I would like to repair, even if only to
} get one to work. Neither advance. One has electronic advance and the other
} doesn't. I have experience in repairing older ultramicrotomes, but I'd
} like to have a set of service instructions, a blueprint, or even a users'
} manual in hand before tearing them apart!
}
} Does anyone have these documents? More importantly, perhaps, advice?
}
} Mahalo,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 14 12:52:37 2004

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Dear Adriana,

as the manufacturer of the KeenView system I would like to provide the
following information:

1) The KeenView has a high anti-bloom factor of about 300. This means that a
pixel can hold 300 times the saturation charge before blooming occurs.

2) A KeenView system is delivered with the analySIS iTEM software, which
includes a Real-time FFT option for focusing and astigmatism correction.

3) Sensitivity is usually not an issue, unless you wish to do low-dose
cryo-TEM. If you let me know what you plan to do, I can see if I have some
images that I could send you.

Please contact me if you have further questions.

Mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: adriana-at-cab.cnea.gov.ar [mailto:adriana-at-cab.cnea.gov.ar]
Sent: Monday, September 13, 2004 17:52
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (adriana-at-cab.cnea.gov.ar) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Monday, September 13, 2004 at 13:56:32
---------------------------------------------------------------------------

Email: adriana-at-cab.cnea.gov.ar
Name: Adriana Cond�

Organization: CONICET, Argentina.

Title-Subject: [Microscopy] [Filtered] MListserver: CCD for TEM in material
science

Question:
Dear microscopists,

We are interested in buying a CCD camera for a Philips CM200 TEM with an
ultratwin lens for HRTEM. The main use is for materials science.
We would like to hear comments on the performance of the KeenView CCD camera
offered by SIS.
We are particularly concerned with the sensibility for HRTEM (for example
typical exposure times) and with the performance of the 12 bit camera for
diffraction pattern acquisition and for online astigmatism correction using
FFT.

All comments will be appreciated.

Thank you for your help.

Adriana

----------------------------------------------------------
Adriana Cond�
CONICET Researcher
Metals Physics Group
Centro At�mico Bariloche
8400 San Carlos de Bariloche
ARGENTINA
e-mail: adriana-at-cab.cnea.gov.ar
Fax: +54 2944 445299
TE: +54 2944 445290
----------------------------------------------------------

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 14 13:50:54 2004

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I don't think water may penetrate epoxy resin easily. The picture, you
described is more like you have water in your sample before plastic. As
far as I could see, the 100% Et-OH step may be a problem in your recipe. 4x
100% Et-OH sounds excessive (I always do 2x20 min 100% Et-OH) - so to me it
looks like that your 100% Et-ON is not a 100% (if you still have water
after 4x). You may need to change the batch or use molecular sieve. You
need also to use at least 1 ml of Et-OH per sample. It's better to do it
in hermetic vials like 1.5 ml Eppendorf tube. If I forgot Et-OH bottle to
close immediately after use, I consider it's 95% Et-OH and use it for
30-95% dehydratation steps only. 3x of PO is sounds excessive too. The
trick here is that as more changes you do, as more your sample has exposed
to water (when you emptied the vial). So, 1 exchange of PO (and Et-OH) is
much better than 3 (you still need 2 for Et-OH) in my point of view. You
need to use huge excess of the solvents - at least 1 ml per sample. As soon
as your sample in PO (or epoxy) - it's protected from water by the layer of
solvent (epoxy). PO and epoxy do not dissolve water, therefore it could
not reach your sample. You still may have a droplets of water (from
condensation for instance) but it'll generate a different picture: round
empty spaces in your sample. You may try also Spurr resin - it may
tolerate a few %% of water in your sample. I hope it'll help, Sergey

At 06:38 AM 9/14/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 14 17:05:46 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (snyderlt-at-newpaltz.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, September 14, 2004 at 08:42:51
---------------------------------------------------------------------------

Email: snyderlt-at-newpaltz.edu
Name: Teresa Snyder-Leiby

Organization: SUNY New Paltz

Title-Subject: [Microscopy] [Filtered] service for philips 300

Question: We have a philips 300 TEM that has been under service contract for the past 4 years. The company is no longer available and I am trying to find a service provider. We are located in New Paltz, NY, about half way between NYC and Albany.

Any suggestions would be greatly appreciated. Teresa

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 14 17:23:10 2004

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If the problem is caused by high humidity in the air, you could try using a
water-miscible resin such as Durcupan or Aquembed. They're both a lot more
expensive than Epon-substitutes and Araldite and they polymerise to a rather
soft block, which may or may not matter depending on your tissue, but it
would eliminate the problem if that is the cause. Another possibility is
that there might still be a trace of PO left, if you don't do one more 100%
in the morning before transferring to the DMP-30 mix, but I don't know why
that would happen only in summer. Hope this helps.

Lesley Weston.

on 13/09/2004 11:24 AM, Garry Burgess at GBurgess-at-exchange.hsc.mb.ca wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
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--} -
}
}
} It seems that during the summer months, we have no end of troubles with our
} plastic not infiltrating the tissue. I've always assumed that this was
} because of the humidity rising quite a bit during the summer months, and the
} poor air conditioning.
}
} It comes out as sections falling apart when I try to flatten them in the
} water boat, or breaking in the electron beam. We have always kept our epoxy
} resin in the freezer in 30 ml plastic syringes, but it usually only gives us
} a problem during the summer months. During the winter here, the air is
} extremely dry.
}
} We do 4 rinses in absolute alcohol, followed by 3 rinses in propylene oxide,
} followed by a 50/50 mixture of epon/araldite mixed with propylene oxide over
} night with the caps off, for the propylene oxide evaporating overnight and
} slowly leaving the specimens in 100% plastic by morning. We polymerize at
} 70 deg C overnight, keeping a dessicant in our embedding oven to keep the
} air as dry as possible too.
}
} Are there any resins which might be used for electron microscopy which are
} more tolerant of water, and which would give a good result with human tissue
} in the electron microscope?
}
} This e-mail and/or any documents in this transmission is intended for the
} address(s) only and may contain legally privileged or confidential
} information. Any unauthorized use, disclosure, distribution, copying or
} dissemination is strictly prohibited. If you receive this transmission in
} error, please notify the sender immediately and return the original.
}

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 14 19:37:02 2004

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I have to take exception to some of the assertions made in a recent response to Garry Burgess' posting. First, propylene oxide is completely miscible with water. And second, Spurr's resin is much more likely to have polymerization problems caused by high humidity than is Epon/Araldite type resin mixtures. Spurr's should be cured either in sealed plastic capsules, or if you prefer to use flat molds, with ample fresh desiccant present. I place the flat molds in a sealed Tupperware container, along with plenty of desiccant. The first time I used Spurr's in flat molds on a humid day, I didn't use a desiccant, and the blocks were too soft to section. I repeated the embedding using a desiccant, and the same batch of chemicals, and the blocks were perfect.

We routinely cure our specimens in Epon/Araldite/DDSA without using a desiccant, and never have problems despite summer humidities of 70% or more. We use 3 x 10m changes of 100% ethanol, 3 x 10m changes in PO, infiltrate overnight in 50% resin, and 8 hours the next day in 100% resin, but always in sealed vials. Leaving vials of 50% resin/PO open to the atmosphere on a humid day will result in water absorption, and might be the cause of the problem. Also, I suspect that the propylene oxide might not be completely removed. We use commercial 100% ethanol from plastic bottles with no problems. As was discussed recently in this forum, use of a molecular sieve can result in particles embedded in your specimen, and damage to diamond knives.

In the recent discussion of the merits of BDMA I was surprised to see that people are pre-mixing their resin with DMP-30 or BDMA, and freezing it. Your resin mix will last much longer (at least a couple of months) if you leave out the accelerator. We mix up large batches of our resin, and freeze aliquots of various sizes in glass vials. When we need the resin, we warm it to room temperature, add the appropriate amount of DMP-30, and mix thoroughly. You may spend a little more time mixing in the DMP, but you won't have to worry about your resin thickening in the freezer.

Ralph Common
Michigan State University
Division of Human Pathology

--------------
Original posting from Garry Burgess:

It seems that during the summer months, we have no end of troubles with our
plastic not infiltrating the tissue. I've always assumed that this was
because of the humidity rising quite a bit during the summer months, and the
poor air conditioning.

It comes out as sections falling apart when I try to flatten them in the
water boat, or breaking in the electron beam. We have always kept our epoxy
resin in the freezer in 30 ml plastic syringes, but it usually only gives us
a problem during the summer months. During the winter here, the air is
extremely dry.

We do 4 rinses in absolute alcohol, followed by 3 rinses in propylene oxide,
followed by a 50/50 mixture of epon/araldite mixed with propylene oxide over
night with the caps off, for the propylene oxide evaporating overnight and
slowly leaving the specimens in 100% plastic by morning. We polymerize at
70 deg C overnight, keeping a dessicant in our embedding oven to keep the
air as dry as possible too.

Are there any resins which might be used for electron microscopy which are
more tolerant of water, and which would give a good result with human tissue
in the electron microscope?

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 14 20:11:54 2004

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margaret

while the thread has been very interesting, your question has been the
most helpful to me. there is a book, it is Hayat's Fixation for
Electron Microscopy. publication date was around 1980. it does not
cover some of the history, but it is an excellent book if you can get
your hands on a copy of it. the help to me....if i can just find that
student who borrowed my copy in june.....

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 14 20:56:29 2004

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Hi, Garry-

I am chronicallly plagued by humidity problems here. Besides making sure
the bottles of absolute ethanol, propylene oxide and resin components, are
opened as little as possible, I put my vials with samples over dessicant
whenever I'm using absolute ethanol, PO, or resin. Always. I have a
rotator we made years ago that accommodates film cans with dessicant into
which my vials fit. I keep dessicant in the embedding oven. And,
imortantly, I pre-heat my molds and labels overnight in the oven before
putting in the samples. This preheating of the capsules or molds and paper
labels also keep bubbles from forming so that I never have to pull a
vacuum on them any more. Yes, it's totally anal, but lots of TEM is!

Good luck!

Aloha,
Tina

} } It seems that during the summer months, we have no end of troubles with our
} } plastic not infiltrating the tissue. I've always assumed that this was
} } because of the humidity rising quite a bit during the summer months, and the
} } poor air conditioning.
} }
} } It comes out as sections falling apart when I try to flatten them in the
} } water boat, or breaking in the electron beam. We have always kept our epoxy
} } resin in the freezer in 30 ml plastic syringes, but it usually only gives us
} } a problem during the summer months. During the winter here, the air is
} } extremely dry.
} }
} } We do 4 rinses in absolute alcohol, followed by 3 rinses in propylene oxide,
} } followed by a 50/50 mixture of epon/araldite mixed with propylene oxide over
} } night with the caps off, for the propylene oxide evaporating overnight and
} } slowly leaving the specimens in 100% plastic by morning. We polymerize at
} } 70 deg C overnight, keeping a dessicant in our embedding oven to keep the
} } air as dry as possible too.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 03:43:01 2004

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Sergey
A propylene oxide layer over the specimen cannot protect against water
penetration.
Water's solubility in 1,2 propylene oxide (=1,2 epoxypropane) is 14.7 wt%.
Conversely, PPO's solubility in water is 40.5 wt%.

Chris

----- Original Message -----
} From: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
To: {Microscopy-at-microscopy.com}
Sent: Tuesday, September 14, 2004 8:18 PM

How effective is molecular sieve as a desiccant at typical polymerization
temperatures (60-70 oC)?? I suspect not very. Would there be a better
choice?

I would also be interested to know what strategies people now recommend for
drying EM solvents
without getting them contaminated with bits of desiccant.

And another thing - how on earth can one determine whether the desiccant is
still being effective?
Can you trust the blue indicator in molecular sieves? What is the
equilibrium water concentration in ethanol above fresh molecular sieve
(assuming the ethanol contained some water initially), and does this
increase when the MS contains, say, 10% water (half its saturation
capacity)?

Best wishes
Chris

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 05:35:21 2004

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Each time I post a message to the list a flurry of autoforwarded replies is
generated - "I am in the Bay area", I am on holiday", "I can be reached at
blah blah!", "I am canoeing down the Danube", Etc.
Although fascinating at a sociological level, this is not maximally useful
web traffic at the EM level. Might I respectfully suggest that listers try
to prevent these messages from reaching the list?

Best regards
Chris

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 07:47:27 2004

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Dear Listservers: We have a CM-10 we wish to do both EDS and low temp work
with. We have the Be Gatan cryo-blades from a 400 but need to adapt the column
phlange to fit the CM column. Neither Gatan nor FEI have been able to resolve
this as yet. Does anyone have any advice or spare phlanges so we can get on
with this? I am thankful for any and all advice I can get. Bob Harris

Guelph Regional Imaging Facility
Dept. of Microbiology
Rm 1199 Thornbrough Bldg.
Univ of Guelph
Guelph,On, Canada
N1G 2W1
ph: 519-824-4120 ext. 56409
Fax: 519-837-1802

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 08:37:29 2004

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Colleagues.....

The Listserver rules ask you to NOT USE "I'm not in the office" autoreplies,
instead you should unsubscribe when you go on vacation or out of town.

If you job requires you to use "out of the office" messages for your work Email
then can I suggest you ask your local system guru to setup a second Email address
for Microscopy which you can recieve all the message at and NOT set this function.

I will remind you that ALL postings are archived and if you miss something
you can find it at:

http://www.microscopy.com/MicroscopyListserver

It is also possible to have multiple addresses subscribed, so there are alot
of ways to mitigate this problem with only a few minutes of your time.
The rest of the community will appreciate your thoughtfulness and consideration.

Cheers..

Nestor
Your Friendly Neighborhood SysOp

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 08:39:54 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tem_iopb-at-iopb.res.in) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 15, 2004 at 05:15:20
---------------------------------------------------------------------------

Email: tem_iopb-at-iopb.res.in
Name: P. V. Satyam

Organization: Institute of Physics, Bhubaneswar,India

Title-Subject: [Microscopy] MListserver: Polymeric Samples: Sample Stage requirements

Question: Dear All,
I would like to do TEM on
(1) Metal/Semiconducting nano-particles dispersed on polymer surfaces
(for example: Ge on PS)
(2)Langmuir-Blodgett Films

We have JEOL 2010 Ultra High Resolution TEM operating at 200kV and LaB6 Filament.

We tried to work at 200 kV using standard double tilt holder (room temperature) but found that polymeric samples started melting upon e-beam irradiation. We dont want to coat any high Z conducting layer on our sample.

As majority of our work (in materials science) is done using 200 kV, I am concerned to work at lower energies.

Does it help having a cooling holder? If so, are there any particulars that should be taken care before purchasing so as enable us to work with polymer and LB films?

I will appreciate your suggestions either directly to me or to the list.

Best regards
Satyam
Institute of Physics, Bhubaneswar, India
Home page: www.iopb.res.in/~tem_iopb

tem_iopb-at-iopb.res.in or satyam-at-iopb.res.in

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From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 08:53:41 2004

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Chris

to avoid the bits in your solvents, try putting your molecular sieve (or whatever) into a length of dialysis tubing. You can fold the ends over and staple them to form a small sausage.

If you get any answers to the rest of your questions I, for one, would be interested to hear them.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
} From: Chris Jeffree {c.jeffree-at-ed.ac.uk}

Hi Chris,

I am out of the office and canoeing on the Current River. I will reply
to your email soon, if I don't drown.

Seriously though, I asked a similar question a while back and was told
that molecular sieves can be put in dialysis tubing to keep them from
chunking up your solvents. Basically, we have quit using molecular
sieves just in case they were responsible for what we thought was
excessive wear on our diamond knives. Since we use a lot of microwave
processing, we generally use acetone, rather than ethanol, and mix our
dehydration series fresh each time. When I use ethanol, I usually
finish up with a recently opened bottle of absolute, but after two or
three uses I relegate that bottle to the 90-95% category, and open up a
new one for the final steps.

This seems to work for us, as we rarely have infiltration problems, even
with retinal tissue which can be problematic.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Wednesday, September 15, 2004 5:40 AM
To: microscopy-at-msa.microscopy.com

How effective is molecular sieve as a desiccant at typical
polymerization temperatures (60-70 oC)?? I suspect not very. Would
there be a better choice?

I would also be interested to know what strategies people now recommend
for drying EM solvents without getting them contaminated with bits of
desiccant.

And another thing - how on earth can one determine whether the desiccant
is still being effective?
Can you trust the blue indicator in molecular sieves? What is the
equilibrium water concentration in ethanol above fresh molecular sieve
(assuming the ethanol contained some water initially), and does this
increase when the MS contains, say, 10% water (half its saturation
capacity)?

Best wishes
Chris

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 09:04:23 2004

Contents Retrieved from Microscopy Listserver Archives
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Malcolm
Thanks for this.I have heard it suggested previously, but I am sceptical
about it.

1) Presumably the dialysis tubing has to be wet before it can be loaded?.
OK, so you can re-dry the MS after loading.
2) I wonder what the permeability of dialysis tubing is to water vapour when
very dry and
immersed in ethanol. It that environment it will be in its most
ultra-compact precipitated state
(since ethanol precipitates polysaccharides from aqueous solution).
3) As in my original question, do you really know that it works, and if so,
from what sort of evidence?

Best wishes
Chris

----- Original Message -----
} From: "Malcolm Haswell" {malcolm.haswell-at-sunderland.ac.uk}
To: "Microscopy MSA" {Microscopy-at-microscopy.com}
Cc: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
Sent: Wednesday, September 15, 2004 3:20 PM

I'm curious about this, how was it used and what explosive was made. By the way Dynamite is not TNT. Dynamite is Nitroglycerin soaked into a diatomaceous earth called Kieselguhr (other absorbents have been used too), this is the explosive invented by Nobel that founded his munitions industry. Dynamite Nobel is still one of the worlds largest munitions makers. TNT is Trinitrotoluene, a different explosive, invented by Wilbrand. For more info on this see: http://en.wikipedia.org/wiki/TNT_(explosive)
and http://en.wikipedia.org/wiki/Dynamite

James L. Roberts
Firearm & Toolmark Examiner
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 654-2308

James.Roberts-at-mail.co.ventura.ca.us

} } } "Sample Prep" {sampleprep-at-earthlink.net} 09/13/04 05:56PM } } }

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Alfred Nobel used Osmium in the production of Explosives in the 1860's. He
is best known for discovering Nitroglycerin & Dynamite (TNT). Now of course
his last will & testament funds "The Nobel Prize".

Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com

----- Original Message -----
} From: {sbarlow-at-sunstroke.sdsu.edu}
To: {microscopy-at-msa.microscopy.com}
Sent: Monday, September 13, 2004 6:56 PM

Chris
Molecular sieve usually used for removing water from the solvents like
Et-OH etc. It has very high capacity and quite effective. Unfortunately
(at my knowledge) molecular sieve does not have indicators signaling it's
time to change sieve. As far as I know, if you add sieve in proportion 10%
from volume, it should work effectively for couple of month (we are talking
about adding molecular sieve to absolute 200 proof ethanol). You may
regenerate molecular sieve at +200-250oC (24-48 h). The disadvantage of
the sieve is that you may have particles in your sample (you right). There
was posting on ListServer a few years ago that people used syringes with
0.22 mkm filters to remove particles. I don't remember details. In my
experience, if I don't disturb solution, it's OK, no particles. Another
popular desiccant is "anhydrous calcium sulfate" - this one do change the
color and may be used for liquids or gases (for some reasons I did not hear
that molecular sieve used for gases). All these materials are capable to
remove the TRACES of water from your solution. If you have 10% water, you
have to distill solvent first and then treat with few portions of
desiccant. Personally, I am using 200 proof ethanol without any
desiccants. I prefer to open new bottle if sample is
important. Otherwise, I am using the bottle a few times as a 100% Et-OH
and then use it for 30-95% dehydratation steps. Have a great day, Sergey

At 03:39 AM 9/15/2004, you wrote:

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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 12:33:31 2004

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Yes it has to be wetted, but just wet the end and pour the sieve in with a funnel. Trim off the wet part and either tie or staple the ends. As for working, I also make a little packet of cupric sulfate the same way as an indicator and it turns blue indicating it has taken up moisture.

Dry it over a flame until it turns white. Recharge when blue. I generally need to do this once a year up here. all my 100% solutions (ethanol, acetone, and HMDS) are on sieve.

Scott Whittaker
Laboratories of Analytical Biology
Smithsonian Institution
National Museum of Natural History
PO Box 37012 MRC104
Washington DC 20013-7012
***New Number202-633-0891***

} } } "Chris Jeffree" {c.jeffree-at-ed.ac.uk} 09/15/04 10:27AM } } }

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Malcolm
Thanks for this.I have heard it suggested previously, but I am sceptical
about it.

1) Presumably the dialysis tubing has to be wet before it can be loaded?.
OK, so you can re-dry the MS after loading.
2) I wonder what the permeability of dialysis tubing is to water vapour when
very dry and
immersed in ethanol. It that environment it will be in its most
ultra-compact precipitated state
(since ethanol precipitates polysaccharides from aqueous solution).
3) As in my original question, do you really know that it works, and if so,
from what sort of evidence?

Best wishes
Chris

----- Original Message -----
} From: "Malcolm Haswell" {malcolm.haswell-at-sunderland.ac.uk}
To: "Microscopy MSA" {Microscopy-at-microscopy.com}
Cc: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
Sent: Wednesday, September 15, 2004 3:20 PM

We have been using molecular sieve in dialysis tubing for at least
20 years as a means of drying our 100% ethanol and acetone. Note that I am
in Florida where the humidity is quite high for many months out of the
year. Dry dialysis tubing can be opened, in order to fill it, by getting a
small opening at one end and then blowing air through it to force it open
all the way. A little blue indicator silica gel can be added for ethanol,
but not acetone, since the dye is acetone soluble.

Greg Erdos, Hurricane Country

} Malcolm
} Thanks for this.I have heard it suggested previously, but I am sceptical
} about it.
}
} 1) Presumably the dialysis tubing has to be wet before it can be loaded?.
} OK, so you can re-dry the MS after loading.
} 2) I wonder what the permeability of dialysis tubing is to water vapour when
} very dry and
} immersed in ethanol. It that environment it will be in its most
} ultra-compact precipitated state
} (since ethanol precipitates polysaccharides from aqueous solution).
} 3) As in my original question, do you really know that it works, and if so,
} from what sort of evidence?
}
} Best wishes
} Chris
}
} ----- Original Message -----
} } From: "Malcolm Haswell" {malcolm.haswell-at-sunderland.ac.uk}
} To: "Microscopy MSA" {Microscopy-at-microscopy.com}
} Cc: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
} Sent: Wednesday, September 15, 2004 3:20 PM
} Subject: [Microscopy] Re: infiltration problems - suitable desiccants
}
}
} } Chris
} }
} } to avoid the bits in your solvents, try putting your molecular sieve (or
} whatever) into a length of dialysis tubing. You can fold the ends over and
} staple them to form a small sausage.
} }
} } If you get any answers to the rest of your questions I, for one, would be
} interested to hear them.
} }
} } Malcolm
} }
} } Malcolm Haswell
} } e.m. unit
} } School of Health, Natural and Social Sciences
} } Fleming Building
} } University of Sunderland
} } Tyne & Wear
} } SR1 3SD
} } UK
} } e-mail: malcolm.haswell-at-sunderland.ac.uk
} }
} } ----- Original Message -----
} } From: Chris Jeffree {c.jeffree-at-ed.ac.uk}
} } Date: Wednesday, September 15, 2004 11:39 am
} } Subject: [Microscopy] infiltration problems - suitable desiccants
} }
} } }
} } }
} } } -------------------------------------------------------------------
} } } -----------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } AmericaTo Subscribe/Unsubscribe --
} } } http://www.msa.microscopy.com/MicroscopyListserverOn-Line Help
} } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html--------
} } } -------------------------------------------------------------------
} } } ----
} } }
} } } How effective is molecular sieve as a desiccant at typical
} } } polymerizationtemperatures (60-70 oC)?? I suspect not very. Would
} } } there be a better
} } } choice?
} } }
} } } I would also be interested to know what strategies people now
} } } recommend for
} } } drying EM solvents
} } } without getting them contaminated with bits of desiccant.
} } }
} } } And another thing - how on earth can one determine whether the
} } } desiccant is
} } } still being effective?
} } } Can you trust the blue indicator in molecular sieves? What is the
} } } equilibrium water concentration in ethanol above fresh molecular sieve
} } } (assuming the ethanol contained some water initially), and does this
} } } increase when the MS contains, say, 10% water (half its saturation
} } } capacity)?
} } }
} } } Best wishes
} } } Chris
} } }
} } }
} } }
} }
} }

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 13:10:43 2004

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I use acetone for my dehydrations (prior to SPurr's infiltration). My
professor taught me years ago (and I teach my students now) to dehydrate
acetone with CuSO4. Place about 50-100g of CuSO4 in an evaporating dish
in a muffle furnace for about 6 hrs. The deep aqua crystals will turn to
a white powder with a faint greenish cast. Allow to cool slightly (in the
furnace), add to an empty bottle, add "100%" acetone from a freshly opened
500 mL bottle, shake, and allow to settle over night. As long as the
copper sulfate does not change color, the acetone is assumed to be
adequately desiccated. I've used this with complete success for acetone,
with not apparent affect on specimens from the copper. I have no idea
whether it works for EtOH.

Don

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 13:39:19 2004

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Hello Listers
I have been reading the replies with great interest because I too
have been struggling with incomplete dehydration/poor infiltration problems
with 7 day zebrafish brain in very dry California. I am using a combination
of microwave and bench processing. My dehydration steps are done under
vacuum on the stedi-temp in the microwave(power level 3, 40 seconds each
100% 1 minute each)and I do 100% ethanol 4x (bottle used only 3 times
before new one is opened, no molecular sieves.)
Moving to 100% acetone and sometimes even PPO and infiltrating with acetone
-resin mixture or PPO/resin. I include 1 overnight step, in 50-50 on
rotator, then 100% for several hours on rotator. I have tried epon/araldite
(frozen with accelerator-made fresh every 3 months) and recently Embed It
(Polysciences Spurr like formulation).
I have white holes or empty areas -sometimes myelin like figures. I have
looked at conventionally prepared tissue that doesn't have it and was
dehydrated for longer periods so I think it is a dehydration problem. The
microwave fixation is far superior to the bench fixed material so I don't
think it can be blamed on the microwave. I have just ordered new acetone in
smaller bottles thinking that maybe my acetone had taken on water. I
process in dram glass vials and always keep the tissue covered.
It is only this darn fish that gives me this trouble. I.have done mouse
brain, Drosophila, rat heart, 5 day fish all using this protocol with great
results. There must be water somewhere but it is hard to track down. This
fish brain is very dense material, along with the skin around it. I dissect
away the eyes and jaw to improve penetration.
Is there anyone out there who has seen this problem before? I'd love some
advice.
Frustrated in California, JoAnn Buchanan

Department of Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 13:43:13 2004

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Dear List;

On the subject of humidity causing poor infiltration ....... several
of my colleagues go from 95% ethanol to Epon substitutes without any
problems. No absolute alcohol, no propylene oxide. Yes, extra changes of
epoxy are needed but the results are fine. Hayat's Prin. and Tech of EM,
second ed., vol 1, page 154 reports that Epon is miscible with 70% ethanol.
I was "raised" with the "you must get every last molecule of water
out of the specimen" dogma but eperience had taught me otherwise. I
suggest you look elsewhere for the cause of your difficulties.

Geoff

Chris Jeffree wrote:

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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 13:56:24 2004

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I want to buy a grinder/polisher that works with Gatan 623 Disc Grinder or SPI
Precision Disc Grinder. Does anyone use "8� Grinder/Polisher" from Electron
Microscopy Sciences with Disc Grinder? Is there any other recommendation? I
would find a grinder around 1-2K.
Thank you,
Hiromi Konishi
Indiana University

Product:
http://www.emsdiasum.com/microscopy/products/materials/polishing.aspx?
mm=9

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 14:10:26 2004

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I've always for the last 20 years used digital balances to measure out
quantities of resin components to mix up plastics, but I'm curious as to why
the manufacturers always specify the amount in volume measures instead of
weight, since it seems to me that it would be a tough job to clean out grad
cylinders full of resin components, vs just filling up a plastic beaker on a
balance, and disposing of it afterwards.

I was just curious as to how others do their measuring, and if they resort
to the horrifying task of cleaning glassware of resin components, which
strikes me as a particularly nasty job.

Garry Burgess

Charge Technologist
Department of Pathology
Health Sciences Centre
Winnipeg, Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 14:25:01 2004

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Dear Satyam,

What, specifically, are you trying to image in these samples? It may be that TEM is not the right tool. An associate of mine has been imaging LB and polymer films with AFM and AFAM (Atomic Force Acoustical Microscopy), with great success and no damage. Contact me off-line if you are interested in details.

Thanks
Barbara Foster
Microscopy/Microscopy Education

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018

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Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

At 09:07 AM 9/15/2004, tem_iopb-at-iopb.res.in wrote:

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From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 16:40:37 2004

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Garry, we measure in volume equivalents, one ml per gram. Although I
recognize that the differing specific gravities of the various
components means that these are not exactly equivalent measures, it
works well for us.

We use 30 ml syringes sans needles to measure the components---pop out
the plunger (and make sure the syringe cap is on!) and fill the body of
the syringe up to the appropriate mark. Reinsert the plunger and push
out the goo into a plastic mixing cup. Repeat for the other components.
Not only is this quick, easy, and about as mess free as working with
resins ever gets, but you get some interesting sound effects that raise
eyebrows on people not in the know. As an added bonus, if you pour the
mixed resin back in the syringes, they make for nice freezer storage and
easy dispensing. When empty, just throw them into an oven until the
remnants are polymerized and toss the used syringes.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Wednesday, September 15, 2004 2:38 PM
To: microscopy-at-microscopy.com

I've always for the last 20 years used digital balances to measure out
quantities of resin components to mix up plastics, but I'm curious as to
why the manufacturers always specify the amount in volume measures
instead of weight, since it seems to me that it would be a tough job to
clean out grad cylinders full of resin components, vs just filling up a
plastic beaker on a balance, and disposing of it afterwards.

I was just curious as to how others do their measuring, and if they
resort to the horrifying task of cleaning glassware of resin components,
which strikes me as a particularly nasty job.

Garry Burgess

Charge Technologist
Department of Pathology
Health Sciences Centre
Winnipeg, Canada

This e-mail and/or any documents in this transmission is intended for
the address(s) only and may contain legally privileged or confidential
information. Any unauthorized use, disclosure, distribution, copying or
dissemination is strictly prohibited. If you receive this transmission
in error, please notify the sender immediately and return the original.

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 16:56:07 2004

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Dear Hiromi,
Because the amount of material removed from 3.05 mm TEM discs with the Gatan
Disc Grinder is very small, I use 600 grit paper stuck to a glass sheet and
carefully hand grind the samples under running water, then finish off with
1200 grit the same way. It only takes a few strokes to remove 50 microns,
then advance the dial and remove another 50 microns. We have spinning wheel
grinders and automatic polishers, but I would not use them for this.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: {hkonishi-at-indiana.edu}
To: {Microscopy-at-microscopy.com}
Sent: Wednesday, September 15, 2004 12:23 PM

Dear Garry,
I found that 1 oz. medicine cups, available from medical suppliers in 1000
cup batches, are disposable and marked off in ml. They hold about 30 ml and
do one or two 1-inch mounts. I use the Tri-pour plastic beakers, which my
Stores man assures me are inexpensive, for larger amounts. I have also heard
that some people use disposable syringes.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca}
To: {microscopy-at-microscopy.com}
Sent: Wednesday, September 15, 2004 12:37 PM

In dense plant tissues, we generally get problems in embedded tissues not
because of incomplete dehydration, but due to infiltration either too fast
or in steps that are too large. If cell walls are moderately impermeable,
then solvent may diffuse out of the tissue faster than resin can diffuse in,
causing tissue collapse and "airspaces" when remaining traces of solvent
within such tissue vapourise during polymerisation. Slower infiltration,
with smaller increases of % resin in solvent, and holding the tissue longer
at each step, has generally solved this problem, for us, anyway.

As an example, a friend found that the single- to few-celled algal zygotes
she was working on had to be infiltrated in increments of 1-2% resin per day
up to 10% or so, after which the increments could be larger, then above 90%
resin, the increments had to be smaller again (don't remember all the
details). If this wasn't done, the zygotes looked like flat balloons after
resin polymerisation.

Not sure if this applies to animal tissues but is another protocol
modification to try....
cheers,
Rosemary

Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 02-6246 5475
CSIRO Plant Industry mob. 0402 835 973
GPO Box 1600 fax. 02-6246 5000
Canberra, ACT 2601

} From: JoAnn Buchanan {redhair-at-stanford.edu}
} Date: Wed, 15 Sep 2004 12:06:49 -0700
} To: {Microscopy-at-msa.microscopy.com}
} Subject: [Microscopy] Infiltration Problems
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
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}
------------------------------------------------------------------------------}
-
}
} Hello Listers
} I have been reading the replies with great interest because I too
} have been struggling with incomplete dehydration/poor infiltration problems
} with 7 day zebrafish brain in very dry California. I am using a combination
} of microwave and bench processing. My dehydration steps are done under
} vacuum on the stedi-temp in the microwave(power level 3, 40 seconds each
} 100% 1 minute each)and I do 100% ethanol 4x (bottle used only 3 times
} before new one is opened, no molecular sieves.)
} Moving to 100% acetone and sometimes even PPO and infiltrating with acetone
} -resin mixture or PPO/resin. I include 1 overnight step, in 50-50 on
} rotator, then 100% for several hours on rotator. I have tried epon/araldite
} (frozen with accelerator-made fresh every 3 months) and recently Embed It
} (Polysciences Spurr like formulation).
} I have white holes or empty areas -sometimes myelin like figures. I have
} looked at conventionally prepared tissue that doesn't have it and was
} dehydrated for longer periods so I think it is a dehydration problem. The
} microwave fixation is far superior to the bench fixed material so I don't
} think it can be blamed on the microwave. I have just ordered new acetone in
} smaller bottles thinking that maybe my acetone had taken on water. I
} process in dram glass vials and always keep the tissue covered.
} It is only this darn fish that gives me this trouble. I.have done mouse
} brain, Drosophila, rat heart, 5 day fish all using this protocol with great
} results. There must be water somewhere but it is hard to track down. This
} fish brain is very dense material, along with the skin around it. I dissect
} away the eyes and jaw to improve penetration.
} Is there anyone out there who has seen this problem before? I'd love some
} advice.
} Frustrated in California, JoAnn Buchanan
}
} Department of Molecular and Cellular Physiology
} Stanford University School of Medicine
} Stanford, CA 94305
} 650-723-5856
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 17:58:07 2004

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Garry

We have used weight measurement for many years. We have a scale in a hood
and use gloves and all disposable containers so dare not too concerned about
the resin components being hazardous. We mix resins as needed rather than
mix large batches and freeze aliquots since it only takes a few minutes to
mix the resins in the quantity we need. This works fine and we get
consistent resin properties in our blocks.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 9/15/04 2:37 PM, "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca} wrote:

}
}
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}
} I've always for the last 20 years used digital balances to measure out
} quantities of resin components to mix up plastics, but I'm curious as to why
} the manufacturers always specify the amount in volume measures instead of
} weight, since it seems to me that it would be a tough job to clean out grad
} cylinders full of resin components, vs just filling up a plastic beaker on a
} balance, and disposing of it afterwards.
}
} I was just curious as to how others do their measuring, and if they resort
} to the horrifying task of cleaning glassware of resin components, which
} strikes me as a particularly nasty job.
}
} Garry Burgess
}
} Charge Technologist
} Department of Pathology
} Health Sciences Centre
} Winnipeg, Canada
}
} This e-mail and/or any documents in this transmission is intended for the
} address(s) only and may contain legally privileged or confidential
} information. Any unauthorized use, disclosure, distribution, copying or
} dissemination is strictly prohibited. If you receive this transmission in
} error, please notify the sender immediately and return the original.
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 19:55:36 2004

Contents Retrieved from Microscopy Listserver Archives
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Dear Hiromi:

The EMS Polisher you reference would certainly be suitable for use with
the disc grinder. Mary Mager makes a good point that a rotating wheel
may not be necessary - or even desirable - if you don't have a lot of
material to remove. I can offer a few suggestions:

Rotating Wheel
If you do require a rotating wheel, our Model 900 Grinder/Polisher is an
enconomical solution that would be suitable for the disc grinder you
describe.

Hand Polishing
If you decide to go with a manual hand polishing process, I would
suggest our Model 180 Lapping Tray. This is essentially a glass plate
mounted in an aluminum base. It is set up to collect waste water and
debris and comes with a cover that allows you to stack multiple trays
each with a different grit size abrasive.

We also have a wide range of other polishing tools and supplies that may
be of interest. I would be pleased to discuss your application with you
in detail off-line.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David

hkonishi-at-indiana.edu wrote:

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--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

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From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 20:34:08 2004

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Dear Hiromi,
Because the amount of material removed from 3.05 mm TEM discs with the Gatan
Disc Grinder is very small, I use 600 grit paper stuck to a glass sheet and
carefully hand grind the samples under running water, then finish off with
1200 grit the same way. It only takes a few strokes to remove 50 microns,
then advance the dial and remove another 50 microns. We have spinning wheel
grinders and automatic polishers, but I would not use them for this.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: {hkonishi-at-indiana.edu}
To: {Microscopy-at-microscopy.com}
Sent: Wednesday, September 15, 2004 12:23 PM

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Garry, we measure in volume equivalents, one ml per gram. Although I
recognize that the differing specific gravities of the various
components means that these are not exactly equivalent measures, it
works well for us.

We use 30 ml syringes sans needles to measure the components---pop out
the plunger (and make sure the syringe cap is on!) and fill the body of
the syringe up to the appropriate mark. Reinsert the plunger and push
out the goo into a plastic mixing cup. Repeat for the other components.
Not only is this quick, easy, and about as mess free as working with
resins ever gets, but you get some interesting sound effects that raise
eyebrows on people not in the know. As an added bonus, if you pour the
mixed resin back in the syringes, they make for nice freezer storage and
easy dispensing. When empty, just throw them into an oven until the
remnants are polymerized and toss the used syringes.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Wednesday, September 15, 2004 2:38 PM
To: microscopy-at-microscopy.com

I've always for the last 20 years used digital balances to measure out
quantities of resin components to mix up plastics, but I'm curious as to
why the manufacturers always specify the amount in volume measures
instead of weight, since it seems to me that it would be a tough job to
clean out grad cylinders full of resin components, vs just filling up a
plastic beaker on a balance, and disposing of it afterwards.

I was just curious as to how others do their measuring, and if they
resort to the horrifying task of cleaning glassware of resin components,
which strikes me as a particularly nasty job.

Garry Burgess

Charge Technologist
Department of Pathology
Health Sciences Centre
Winnipeg, Canada

This e-mail and/or any documents in this transmission is intended for
the address(s) only and may contain legally privileged or confidential
information. Any unauthorized use, disclosure, distribution, copying or
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From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 20:34:25 2004

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Garry

We have used weight measurement for many years. We have a scale in a hood
and use gloves and all disposable containers so dare not too concerned about
the resin components being hazardous. We mix resins as needed rather than
mix large batches and freeze aliquots since it only takes a few minutes to
mix the resins in the quantity we need. This works fine and we get
consistent resin properties in our blocks.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 9/15/04 2:37 PM, "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca} wrote:

}
}
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
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}
}
} I've always for the last 20 years used digital balances to measure out
} quantities of resin components to mix up plastics, but I'm curious as to why
} the manufacturers always specify the amount in volume measures instead of
} weight, since it seems to me that it would be a tough job to clean out grad
} cylinders full of resin components, vs just filling up a plastic beaker on a
} balance, and disposing of it afterwards.
}
} I was just curious as to how others do their measuring, and if they resort
} to the horrifying task of cleaning glassware of resin components, which
} strikes me as a particularly nasty job.
}
} Garry Burgess
}
} Charge Technologist
} Department of Pathology
} Health Sciences Centre
} Winnipeg, Canada
}
} This e-mail and/or any documents in this transmission is intended for the
} address(s) only and may contain legally privileged or confidential
} information. Any unauthorized use, disclosure, distribution, copying or
} dissemination is strictly prohibited. If you receive this transmission in
} error, please notify the sender immediately and return the original.
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 20:34:19 2004

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In dense plant tissues, we generally get problems in embedded tissues not
because of incomplete dehydration, but due to infiltration either too fast
or in steps that are too large. If cell walls are moderately impermeable,
then solvent may diffuse out of the tissue faster than resin can diffuse in,
causing tissue collapse and "airspaces" when remaining traces of solvent
within such tissue vapourise during polymerisation. Slower infiltration,
with smaller increases of % resin in solvent, and holding the tissue longer
at each step, has generally solved this problem, for us, anyway.

As an example, a friend found that the single- to few-celled algal zygotes
she was working on had to be infiltrated in increments of 1-2% resin per day
up to 10% or so, after which the increments could be larger, then above 90%
resin, the increments had to be smaller again (don't remember all the
details). If this wasn't done, the zygotes looked like flat balloons after
resin polymerisation.

Not sure if this applies to animal tissues but is another protocol
modification to try....
cheers,
Rosemary

Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 02-6246 5475
CSIRO Plant Industry mob. 0402 835 973
GPO Box 1600 fax. 02-6246 5000
Canberra, ACT 2601

} From: JoAnn Buchanan {redhair-at-stanford.edu}
} Date: Wed, 15 Sep 2004 12:06:49 -0700
} To: {Microscopy-at-msa.microscopy.com}
} Subject: [Microscopy] Infiltration Problems
}
}
}
} ------------------------------------------------------------------------------
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}
} Hello Listers
} I have been reading the replies with great interest because I too
} have been struggling with incomplete dehydration/poor infiltration problems
} with 7 day zebrafish brain in very dry California. I am using a combination
} of microwave and bench processing. My dehydration steps are done under
} vacuum on the stedi-temp in the microwave(power level 3, 40 seconds each
} 100% 1 minute each)and I do 100% ethanol 4x (bottle used only 3 times
} before new one is opened, no molecular sieves.)
} Moving to 100% acetone and sometimes even PPO and infiltrating with acetone
} -resin mixture or PPO/resin. I include 1 overnight step, in 50-50 on
} rotator, then 100% for several hours on rotator. I have tried epon/araldite
} (frozen with accelerator-made fresh every 3 months) and recently Embed It
} (Polysciences Spurr like formulation).
} I have white holes or empty areas -sometimes myelin like figures. I have
} looked at conventionally prepared tissue that doesn't have it and was
} dehydrated for longer periods so I think it is a dehydration problem. The
} microwave fixation is far superior to the bench fixed material so I don't
} think it can be blamed on the microwave. I have just ordered new acetone in
} smaller bottles thinking that maybe my acetone had taken on water. I
} process in dram glass vials and always keep the tissue covered.
} It is only this darn fish that gives me this trouble. I.have done mouse
} brain, Drosophila, rat heart, 5 day fish all using this protocol with great
} results. There must be water somewhere but it is hard to track down. This
} fish brain is very dense material, along with the skin around it. I dissect
} away the eyes and jaw to improve penetration.
} Is there anyone out there who has seen this problem before? I'd love some
} advice.
} Frustrated in California, JoAnn Buchanan
}
} Department of Molecular and Cellular Physiology
} Stanford University School of Medicine
} Stanford, CA 94305
} 650-723-5856
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 20:41:51 2004

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Garry

We have always measured our Spurr's resin components by weight, most of the recipes for this resin seem to do this. I agree most of the literature on other resins seems to quote volumes though in the latest edition of Glauerts book on Biological Specimen Preparation weight equivalents are given (proportions are close to but not identical to those using 1g = 1ml)

The reference is
Biological Specimen Preparation for Transmission Electron Microscopy - Audrey M. Glauert and Peter R. Lewis
Practical Methods in Electron Microscopy: Vol 17
Portland Press London 1998

The earlier version of this (1974) only seems to note volumes.

Ian

Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz

} } } Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 16/09/2004 7:37:56 a.m. } } }

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I've always for the last 20 years used digital balances to measure out
quantities of resin components to mix up plastics, but I'm curious as to why
the manufacturers always specify the amount in volume measures instead of
weight, since it seems to me that it would be a tough job to clean out grad
cylinders full of resin components, vs just filling up a plastic beaker on a
balance, and disposing of it afterwards.

I was just curious as to how others do their measuring, and if they resort
to the horrifying task of cleaning glassware of resin components, which
strikes me as a particularly nasty job.

Garry Burgess

Charge Technologist
Department of Pathology
Health Sciences Centre
Winnipeg, Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 23:12:08 2004

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It works perfectly with Et-OH and this is exactly how they teach me. Yes,
CuSO4 - it was many years ago. Thanks for reminding. Sergey

At 11:37 AM 9/15/2004, you wrote:

} ------------------------------------------------------------------------------
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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 23:20:35 2004

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Chris, you right.
I am sorry, I was surely believe that PPO does not soluble in the
water. So, my theory that PPO may "protect" sample from the water is
wrong. From another hand I never used desiccants with PPO. PPO comes in
0.5 liter cans and I opened it frequently. Nevertheless, I did not have
problems with water. Anyway, thanks for clarification and have a great
day/night. Sergey

At 02:06 AM 9/15/2004, you wrote:
} Sergey
} A propylene oxide layer over the specimen cannot protect against water
} penetration.
} Water's solubility in 1,2 propylene oxide (=1,2 epoxypropane) is 14.7 wt%.
} Conversely, PPO's solubility in water is 40.5 wt%.
}
} Chris
}
} ----- Original Message -----
} From: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
} To: {Microscopy-at-microscopy.com}
} Sent: Tuesday, September 14, 2004 8:18 PM
} Subject: [Microscopy] Re: RE: Infiltration Problems
}
}
} }
} }
} } --------------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
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} -----
} }
} } I don't think water may penetrate epoxy resin easily. The picture, you
} } described is more like you have water in your sample before plastic. As
} } far as I could see, the 100% Et-OH step may be a problem in your recipe.
} 4x
} } 100% Et-OH sounds excessive (I always do 2x20 min 100% Et-OH) - so to me
} it
} } looks like that your 100% Et-ON is not a 100% (if you still have water
} } after 4x). You may need to change the batch or use molecular sieve. You
} } need also to use at least 1 ml of Et-OH per sample. It's better to do it
} } in hermetic vials like 1.5 ml Eppendorf tube. If I forgot Et-OH bottle to
} } close immediately after use, I consider it's 95% Et-OH and use it for
} } 30-95% dehydratation steps only. 3x of PO is sounds excessive too. The
} } trick here is that as more changes you do, as more your sample has exposed
} } to water (when you emptied the vial). So, 1 exchange of PO (and Et-OH) is
} } much better than 3 (you still need 2 for Et-OH) in my point of view. You
} } need to use huge excess of the solvents - at least 1 ml per sample. As
} soon
} } as your sample in PO (or epoxy) - it's protected from water by the layer
} of
} } solvent (epoxy). PO and epoxy do not dissolve water, therefore it could
} } not reach your sample. You still may have a droplets of water (from
} } condensation for instance) but it'll generate a different picture: round
} } empty spaces in your sample. You may try also Spurr resin - it may
} } tolerate a few %% of water in your sample. I hope it'll help, Sergey
} }
} } At 06:38 AM 9/14/2004, you wrote:
} }
} }
} }
} } ---------------------------------------------------------------------------
} ---
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
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} }
} } ---------------------------------------------------------------------------
} ----
} } }
} } } }
} } } } It seems that during the summer months, we have no end of
} } } } troubles with our plastic not infiltrating the tissue. I've
} } } } always assumed that this was because of the humidity rising
} } } } quite a bit during the summer months, and the poor air conditioning.
} } } }
} } } } It comes out as sections falling apart when I try to flatten
} } } } them in the water boat, or breaking in the electron beam. We
} } } } have always kept our epoxy resin in the freezer in 30 ml
} } } } plastic syringes, but it usually only gives us a problem
} } } } during the summer months. During the winter here, the air is
} } } } extremely dry.
} } } }
} } } } We do 4 rinses in absolute alcohol, followed by 3 rinses in
} } } } propylene oxide, followed by a 50/50 mixture of epon/araldite
} } } } mixed with propylene oxide over night with the caps off, for
} } } } the propylene oxide evaporating overnight and slowly leaving
} } } } the specimens in 100% plastic by morning. We polymerize at
} } } } 70 deg C overnight, keeping a dessicant in our embedding oven
} } } } to keep the air as dry as possible too.
} }
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } 10833 Le Conte Ave, Room 33-080
} } Los Angeles, CA 90095
} }
} } Phone: (310) 825-1144 (office)
} } (310) 206-1029 (Lab)
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
} }

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 23:30:23 2004

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Dear Colleagues:

I�m working with some extremely fastidious
dinoflagelates, which I can�t seam to keep the
flagella in place. I�m after the flagella attachment
of the Pyrodinium Bahamense. On a separate batch I�m
looking to break the armor to take a look inside. I
have modified the dehydration process in order to see
if that preserve the flagella. I�m also thinking to
change the fixative from Paraformaldehyde to
Glutaraldehyde. Any ideas into how to treat these
organisms will be greatly appreciated.

Omayra Velez
New Jersey

__________________________________________________
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Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 15 23:34:33 2004

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Dear Colleagues:

I'm sorry for the previous email subject heading. It
didnot make any sense. Originaly, I had an osmium
question, about the dinoflagellates but I found the
answer myself.

Omayra Velez
New Jersey

__________________________________________________
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Tired of spam? Yahoo! Mail has the best spam protection around
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From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 08:01:18 2004

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Just to add to you potential choices Ladd offers our L900 8"
Grinder/Polisher.

JD Arnott

Disclaimer: Ladd Research is in the business of supplying your microscopy
needs including the products mentioned in this thread.

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: ladres-at-att.net

----- Original Message -----
} From: {hkonishi-at-indiana.edu}
To: {Microscopy-at-microscopy.com}
Sent: Wednesday, September 15, 2004 3:23 PM

I want to buy a grinder/polisher that works with Gatan 623 Disc Grinder or
SPI
Precision Disc Grinder. Does anyone use "8" Grinder/Polisher" from Electron
Microscopy Sciences with Disc Grinder? Is there any other recommendation? I
would find a grinder around 1-2K.
Thank you,
Hiromi Konishi
Indiana University

Product:
http://www.emsdiasum.com/microscopy/products/materials/polishing.aspx?
mm=9

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: ladres-at-att.net

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 08:12:18 2004

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It's not hard to measure volumes when you use a syringe.

Mannie Steglich
UT MDACC

Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}

09/15/2004 02:37 PM

To:
{microscopy-at-microscopy.com}
cc:

I've always for the last 20 years used digital balances to measure out
quantities of resin components to mix up plastics, but I'm curious as to
why
the manufacturers always specify the amount in volume measures instead of
weight, since it seems to me that it would be a tough job to clean out
grad
cylinders full of resin components, vs just filling up a plastic beaker on
a
balance, and disposing of it afterwards.

I was just curious as to how others do their measuring, and if they resort
to the horrifying task of cleaning glassware of resin components, which
strikes me as a particularly nasty job.

Garry Burgess

Charge Technologist
Department of Pathology
Health Sciences Centre
Winnipeg, Canada

This e-mail and/or any documents in this transmission is intended for the
address(s) only and may contain legally privileged or confidential
information. Any unauthorized use, disclosure, distribution, copying or
dissemination is strictly prohibited. If you receive this transmission in
error, please notify the sender immediately and return the original.

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 08:44:56 2004

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Debby,

I only freeze a small amount and only use the stored frozen aliquots for the
intermediate stages: the mixes with propylene oxide. I always use freshly
mixed resin for embedding.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Wednesday, September 15, 2004 7:25 PM
To: Garry Burgess; microscopy-at-microscopy.com

Garry

We have used weight measurement for many years. We have a scale in a hood
and use gloves and all disposable containers so dare not too concerned about
the resin components being hazardous. We mix resins as needed rather than
mix large batches and freeze aliquots since it only takes a few minutes to
mix the resins in the quantity we need. This works fine and we get
consistent resin properties in our blocks.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 9/15/04 2:37 PM, "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
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}
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-
}
}
} I've always for the last 20 years used digital balances to measure out
} quantities of resin components to mix up plastics, but I'm curious as to why
} the manufacturers always specify the amount in volume measures instead of
} weight, since it seems to me that it would be a tough job to clean out grad
} cylinders full of resin components, vs just filling up a plastic beaker on a
} balance, and disposing of it afterwards.
}
} I was just curious as to how others do their measuring, and if they resort
} to the horrifying task of cleaning glassware of resin components, which
} strikes me as a particularly nasty job.
}
} Garry Burgess
}
} Charge Technologist
} Department of Pathology
} Health Sciences Centre
} Winnipeg, Canada
}
} This e-mail and/or any documents in this transmission is intended for the
} address(s) only and may contain legally privileged or confidential
} information. Any unauthorized use, disclosure, distribution, copying or
} dissemination is strictly prohibited. If you receive this transmission in
} error, please notify the sender immediately and return the original.
}

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 08:51:56 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy, et al,

I prepare my resin exactly the same way, Randy, and have not had any problems.
Have raised a few eyebrows with the sound effects also!
I store small aliquots of resin in scintillation vials in the freezer. I don't
process a high volume of tissue, so for me this method works well. I just thaw
a vial and use a plastic disposable pipet to fill my molds. No fuss, no mess.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Wednesday, September 15, 2004 6:07 PM
To: Garry Burgess
Cc: microscopy-at-microscopy.com

Garry, we measure in volume equivalents, one ml per gram. Although I
recognize that the differing specific gravities of the various
components means that these are not exactly equivalent measures, it
works well for us.

We use 30 ml syringes sans needles to measure the components---pop out
the plunger (and make sure the syringe cap is on!) and fill the body of
the syringe up to the appropriate mark. Reinsert the plunger and push
out the goo into a plastic mixing cup. Repeat for the other components.
Not only is this quick, easy, and about as mess free as working with
resins ever gets, but you get some interesting sound effects that raise
eyebrows on people not in the know. As an added bonus, if you pour the
mixed resin back in the syringes, they make for nice freezer storage and
easy dispensing. When empty, just throw them into an oven until the
remnants are polymerized and toss the used syringes.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Wednesday, September 15, 2004 2:38 PM
To: microscopy-at-microscopy.com

I've always for the last 20 years used digital balances to measure out
quantities of resin components to mix up plastics, but I'm curious as to
why the manufacturers always specify the amount in volume measures
instead of weight, since it seems to me that it would be a tough job to
clean out grad cylinders full of resin components, vs just filling up a
plastic beaker on a balance, and disposing of it afterwards.

I was just curious as to how others do their measuring, and if they
resort to the horrifying task of cleaning glassware of resin components,
which strikes me as a particularly nasty job.

Garry Burgess

Charge Technologist
Department of Pathology
Health Sciences Centre
Winnipeg, Canada

This e-mail and/or any documents in this transmission is intended for
the address(s) only and may contain legally privileged or confidential
information. Any unauthorized use, disclosure, distribution, copying or
dissemination is strictly prohibited. If you receive this transmission
in error, please notify the sender immediately and return the original.

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 08:54:25 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dinoflagellates are not easy to fix properly. My experience with them has been that each species (especially the lightly armored or unarmored ones)can have their own individual reqirements for fixation. When I have problems with fixation I use what has been called Parducz fixative. It was originally developed in the 1960's for work on cilliates by Bela Parducz to study the metachronal wave pattern of the cillia. It consists of a mixture of Osmium tetroxide and mercuric chloride. To prepare the solution according to Parducz, you make a 6:1(V:V) mixture of 2% aqueous Osmium tetroxide(OsO4) with saturated aqueous mercuric chloride(HgCl2). You can adjust the relative mixtures of the two components as I know that I have used 3:2 and perhaps 1:1. This fixative has been referred to as an "instantaneous fix" as it will fix the specimens very, very, rapidly. Parducz fixative was the only way that I could prepare some dinoflagellates for SEM and retain not only their morphology, but flagella as well. It is not as good a fix for TEM. Here are a couple of references that you may want to see:

Small, E.B. 1968. Scanning electron microscopy of fixed, frozen and dried
} protozoa. Science. 163:1064-1065.
}
} and
}
} Bela Parducz. 1966. Ciliary Movement abd Coordination in Cilliates.
} International Review of Cytology. 21:91-128.

Omayra Velez wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 09:10:45 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List:

The July/August issue of PhotoTechniquesUSA has a review of the HP
Photosmart 7960 printer. The reviewer, a professional photographer and
printmaker named Ctein, said "it makes the best (and most permanent)
black and white prints of any computer printer I've ever tried." It is
an 8 ink printer that has 3 photo gray inks in addition to the usual
color inks although it seems that the photo gray ink cartridge is an
extra cost option?
The magazine has a website http://www.phototechmag.com/ but I do
not know if the review is available there.

Geoff

Gary Gaugler wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} I was a fan of Epson photo printers for quite
} some time. Notable was the 890 & 980. It is a
} small format printer compared to the 2200.
} I recently (a year or so ago) bought a Epson Stylus
} Photo 2000. It lasted about six months and
} then jammed constantly. In-warranty customer
} service and any idea of repair was on a wish list.
} It never happened. The printer was scrapped.
}
} The 2200 may have solved teething problems
} with large format printers. However, the 2000
} was VERY slooooow using photo paper. When it
} worked, the results were stunning. Many times
} (too many) it would stop printing 1/4 or 1/2
} way through the print and just die. The job
} hung (Win2K Pro) and had to be restarted with
} a new sheet of paper.
}
} The Epson and Canon small format printers seem
} to do a better, more reliable job. As a result
} of being burned by Epson, I now take print jobs
} to a local service bureau. they do a very nice
} job for not much cost. These are mostly for
} 24" x 48" glossy mounted prints. Small ones
} are done on my HP 4550 color laser printer.
} If the color gamut is matched well between
} the monitor and Photoshop, the HP does a nice job
} for reports. For transparencies (not much used
} any longer), the Kodak dye sub is excellent.
}
} Let us know what you find. There are a lot
} of options. Also, check out the Ethernet print
} servers that will connect a non-network printer
} to a LAN and allow all to use it. HP and others
} make these. they usually cost about $100 or so.
}
} gary g.
}
}
} At 11:08 AM 9/2/2004, you wrote:
}
} } Email: jtd1-at-psu.edu
} } Name: Tom Doman
} }
} } Organization: Penn State University
} }
} } Title-Subject: [Microscopy] [Filtered] MListserver:
} }
} } Question: Our lab is considering various photo printers (} $1K) for
} } production of electron micrographs. Currently the Epson 2200 is the
} } stromg favorite. Are there any recomendations for other printers
} } which we should consider? What are your reasons for the recomended
} } printer?
} }
} } Thanks in advance!
} }
} } Tom
} }
} } ---------------------------------------------------------------------------
} }
}
}
}

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 10:44:57 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greg, Omayra

i wonder if the following is relevant - narcotize the critters before fixing
them.
see, for example:

May L. (1985) The use of procaine hydrochloride in the preparation
of rotifer samples for counting. Verh.Internat.Verein.Limnol. 22, 2987-2990.

Abstract: Although many rotifer species are easily recognized when
killed and fixed in formalin, some of the soft-bodied forms contract
violently on contact with the chemical and become unrecognizable.
In samples from Loch Leven this situation occurred most commonly with
Synchaeta kitina Rousselet and several possible methods of killing
the species in a relaxed from were considered (May, 1980).
Narcotizing the animals with procaine hydrochloride
(NH2.C6H4.COO.CH2.CH2.N(C2H5)2.HCl) before adding formalin was
found to be the most succesful method. This paper describes the
development of the method and examines its effect on rotifer
density estimates

Chris

----- Original Message -----
} From: "Greg Strout" {gstrout-at-ou.edu}
To: "Omayra Velez" {mayas003-at-yahoo.com}
Cc: {Microscopy-at-MSA.Microscopy.com}
Sent: Thursday, September 16, 2004 3:21 PM

I am looking for a "micrometer" that can work with optical microscope. I think
that it read the difference of dial for focusing and convert to actual
distance (um). I want to use it for determining the thickness of disc during
disc grinding if it is not so expensive.
Please advise on website or product information.
Thank you,

Hiromi Konishi
Indiana University

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 11:06:46 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

List,

I own 2 of these printers and they actually come with all 4 cartridges,
color, photo, black & grey. The unit has a storage compartment for the
cartridge not in use (either the grey or the black). I agree with the
review it does an outstanding job of both color and B&W. When printing
at its highest resolution an 8X10 print can take 11 minutes to print
and will need (temporarly) 400 Mb of hard disk space. When I got mine
the were selling at 300 USD, they are now selling at about 200 USD.

Love 'em
Robert Schoonhoven

Geoff McAuliffe wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Dear List:
}
} The July/August issue of PhotoTechniquesUSA has a review of the HP
} Photosmart 7960 printer. The reviewer, a professional photographer and
} printmaker named Ctein, said "it makes the best (and most permanent)
} black and white prints of any computer printer I've ever tried." It is
} an 8 ink printer that has 3 photo gray inks in addition to the usual
} color inks although it seems that the photo gray ink cartridge is an
} extra cost option?
} The magazine has a website http://www.phototechmag.com/ but I do
} not know if the review is available there.
}
} Geoff
}
} Gary Gaugler wrote:
}
} }
} }
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} }
} } I was a fan of Epson photo printers for quite
} } some time. Notable was the 890 & 980. It is a
} } small format printer compared to the 2200.
} } I recently (a year or so ago) bought a Epson Stylus
} } Photo 2000. It lasted about six months and
} } then jammed constantly. In-warranty customer
} } service and any idea of repair was on a wish list.
} } It never happened. The printer was scrapped.
} }
} } The 2200 may have solved teething problems
} } with large format printers. However, the 2000
} } was VERY slooooow using photo paper. When it
} } worked, the results were stunning. Many times
} } (too many) it would stop printing 1/4 or 1/2
} } way through the print and just die. The job
} } hung (Win2K Pro) and had to be restarted with
} } a new sheet of paper.
} }
} } The Epson and Canon small format printers seem
} } to do a better, more reliable job. As a result
} } of being burned by Epson, I now take print jobs
} } to a local service bureau. they do a very nice
} } job for not much cost. These are mostly for
} } 24" x 48" glossy mounted prints. Small ones
} } are done on my HP 4550 color laser printer.
} } If the color gamut is matched well between
} } the monitor and Photoshop, the HP does a nice job
} } for reports. For transparencies (not much used
} } any longer), the Kodak dye sub is excellent.
} }
} } Let us know what you find. There are a lot
} } of options. Also, check out the Ethernet print
} } servers that will connect a non-network printer
} } to a LAN and allow all to use it. HP and others
} } make these. they usually cost about $100 or so.
} }
} } gary g.
} }
} }
} } At 11:08 AM 9/2/2004, you wrote:
} }
} } } Email: jtd1-at-psu.edu
} } } Name: Tom Doman
} } }
} } } Organization: Penn State University
} } }
} } } Title-Subject: [Microscopy] [Filtered] MListserver:
} } }
} } } Question: Our lab is considering various photo printers (} $1K) for
} } } production of electron micrographs. Currently the Epson 2200 is the
} } } stromg favorite. Are there any recomendations for other printers
} } } which we should consider? What are your reasons for the recomended
} } } printer?
} } }
} } } Thanks in advance!
} } }
} } } Tom
} } }
} } } ---------------------------------------------------------------------------
} } }
} }
} }
} }
} }
}

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 11:05:30 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If a rotating platen is used with a hand grinder, the flat surface of the hand grinder is usually rounded off and can become facetted. However, you can use a polishing station, just don't turn it on; keep it stationary. I have used the South Bay Technology Lapping tray with all of the common hand grinders, South Bay's, Fischione's, and Gatan's. This is my preferred way of thinning TEM samples down to 100 um or less. I have also used the Gatan lapping tray. I found this one too small and I don't like using the PSA backing on the disks (besides the high costs of the disks). With the lapping tray, I use 8" SiC disks without the PSA backing and hold them down by hand. They don't last long enough to go through the hassle of pasting them down. In addition, the lapping tray works very well with the 3M diamond films and they can last a very long time depending on your material. For III-V compounds, I have used a single 30 um disk for months. These work well, because the water used
for lubrication can be kept on the pad because they are hydrophobic. (Careful, not all diamond films are the same and other brands are not hydrophobic and have this property.) They stay put when water is used to hold them down. As in the Tripod(TM) polishing technique, you hold them down by putting a puddle of water on the plate and then carefully put the pad down moving it around so that no air bubbles get under the pad and then squeegee the water out. It will stay put like that for over 1/2 hr if you do not let any water get under it.

A word about the glass used in the lapping tray, since I work at the Glass Technology Center. Glass has two sides, the air side and the tin side. The tin side is the smoothest and is less sensitive to corrosion. You can tell the Sn side because it will fluoresce under UV. If you can determine it, this is the side that you should put your 3M papers on. You do not want water with any of the ground material to dry on the glass. If there are any silicates present, they will bond with the glass when it dries and you will not be able to remove it. I always use distilled water with the lapping trays, but that may not always be possible.

Another word of caution, don't drop your hand grinder on the glass plate! Not everyone can replace it as easily as I did.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)

-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Wednesday, September 15, 2004 6:23 PM
To: hkonishi-at-indiana.edu
Cc: Microscopy

Dear Hiromi,
Because the amount of material removed from 3.05 mm TEM discs with the Gatan
Disc Grinder is very small, I use 600 grit paper stuck to a glass sheet and
carefully hand grind the samples under running water, then finish off with
1200 grit the same way. It only takes a few strokes to remove 50 microns,
then advance the dial and remove another 50 microns. We have spinning wheel
grinders and automatic polishers, but I would not use them for this.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: {hkonishi-at-indiana.edu}
To: {Microscopy-at-microscopy.com}
Sent: Wednesday, September 15, 2004 12:23 PM

Members,
In my opinion, the HP 7960 is a great photo printer. It has it all -
dedicated gray level ink cartridge, tiny ink droplet size, low cost ( { $200
at the typical warehouse club stores) and has all of the various memory card
slots on the front panel for your digital camera along with a color LCD
display for previewing the images. Even on standard copy paper, the images
really come out nice.

Disclaimer:
I do not work for HP nor have any financial interest in said company. I
do however, bundle this printer (at the end user's request) with the SEM
Digital Image Capture Systems that I do sell.

Gary M. Easton
Scanners Corporation
Third Party SEM Service/Digital Imaging/EDS Sales
410-857-7633 x102

----- Original Message -----
} From: "Geoff McAuliffe" {mcauliff-at-umdnj.edu}
To: "Gary Gaugler" {gary-at-gaugler.com}
Cc: "by way of MicroscopyListserver" {jtd1-at-psu.edu} ; "MSA listserver"
{Microscopy-at-MSA.Microscopy.Com}
Sent: Thursday, September 16, 2004 1:33 PM

Recently I acquired an HP 6540 printer, not a photo printer as such (no built in card reader or LCD screen), but I have been extremely impressed with its quality and speed in printing my SEM images (formerly used an HP 970). It is very quick - prints two 4x5" photos on one sheet of HP Premium inkjet paper in 30 seconds (standard print quality setting). I compared the output quality with printing at the top quality setting and found little if any visible improvement for the doubling in output time.

It also takes gray or photo color cartridges in place of the black one. I have not been impressed with either of these, the colors seem weak and don't match the ones on my monitor, which are gray scale. So for all that I'm sticking with the standard black and color cartridges for the best results here.

I had been looking at the HP 7960, but purchasing wrinkles pointed me to the 6540 and I'm happy they did. Was a bit cheaper too ($150).

Richard Shalvoy
Arch Chemicals, Inc.
350 Knotter Drive
Cheshire, CT 06410
(203) 271-4394
rbshalvoy-at-archchemicals.com

-----Original Message-----
} From: rschoon [mailto:rschoon-at-email.unc.edu]
Sent: Thursday, September 16, 2004 12:32 PM
To: Geoff McAuliffe
Cc: Gary Gaugler; by way of MicroscopyListserver; MSA listserver

Hi Omayra,
We have used Parducz since 1967 and it works wonders on bacteria,
protozoa, and many other critters especially those with flagella and
cilia. Two things that we have found important in its use are:
1. Make it FRESH just before use
We keep a saturated solution of HgCl2 around in a brown 50 ml bottle
which has been as old as one year. To make it we put a bit of HgCl2 into
distilled water until we see that it is saturated i.e. with a ppt on the
bottom. We also keep a bottle of 2% aq OsO4 in the refrigerator (usually
double jarred to prevent vapors from escaping).
JUST before use then we mix 6 ml of 2% OsO4 and 1 ml of the saturated
HgCl2 (taken from the top of the bottle). We then fix whatever we are
fixing for an hour or so. Obviously if more fix is needed, larger
amounts can be made.
2. Wash WELL after the Parducz fixation. Otherwise you get starfish
shaped crystals on the surface of your prep. They may look neat but
obviously are not part of the specimen.
Other than that, it is a great hardening fix for SEM.

Good Luck,
Judy Murphy
Stockton, CA

Omayra Velez wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 13:30:40 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Hiromi,
A good solution would be a linear encoder for upright microscopes with a
digital readout. We use a unit from Hiedenhain
(www.heidenhain.com/product.html) on our stereology rig. There may be a
way to set one up for inverted bases as well. Digital length gauge
systems or linear encoders can measure z position with very high
precision. You may wish to contact the folks at MicroBrightfield
(www.microbrightfield.com) as they sell such solutions specifically for
microscopes.
Simply calibrating the markings on focus knobs to displacement of
the stage in z may not be very precise in some instances. Older
axiovert bases have a friction based focusing system which can slip
depending on the speed or distance through which the knob is turned.
Other bases may have similar issues with the focus mechanism.
Calibration strategies in which the top and bottom of a z calibration
standard are imaged to determine endpoints will have to take into
account the uncertainty due to depth of focus of a particular objective
lens as well. The linear encoder avoids this uncertaintly.
Regards,
Karl

hkonishi-at-indiana.edu wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 14:08:21 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We just purchased a Canon i9900. The initial test prints are beautiful. It
uses eight cartridges and when using Canon's Pro Paper, you would not know
that they are computer prints rather than high quality true photographs. We
elected to have a larger format capacity (13" x 19") as we like to decorate
our lab and office walls with our favorites.

Al Stone
ASTON Metallurgical Services

At 01:02 PM 9/16/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 16:20:53 2004

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Dear All EM Specialists:

I really enjoy being on this forum and am educated a lot. Now I have a question about how often we need to flash our field emission SEM's tip.

We have a brand new Hitachi 4700 which has a cold field emission tip. When it was first installed, the service engineer told us to record the initial extraction voltage each time right after flashing, during the use if the increase of extraction voltage is more than 1.4 KV, we need to do a flash. We follow this rule strictly. Several weeks ago Hitachi's application engineer visited us and said by doing that we flashed too much and would short our tip's lifetime. We do not need to flash until we see the tip noise. I never see any tip noise in my image by following his suggestion so I am forced to flash by the instrument setting (every 48 hours).

Could you please kindly share your experience with me?

Thanks

Ying Shi

Analytic Scientist
Delphi Catalyst
ying.shi-at-delphi.com

****************************************************************************************

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From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 16:28:53 2004

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Hello All,

I have a BioScan Ted Pella microwave with a cold-spot and vacuum chamber
that I would like to use for fixation and embedding insect eggs and larvae
for TEM.

The project includes mosquito larvae, pupae and fly eggs. I worked on this
same project many years ago using traditional benchtop methods. The eggs
and larvae were difficult, if not impossible, to infiltrate. Now that I
have a MW with vacuum I am trying this project again.

I would greatly appreciate any suggestions, advice or direct me to references?

My first attempt at MW didn't go so well.
I MW fixed the larvae with 2% glut in cacodylate in 1.5ml centrifuge tubes
at 20mmHg vacuum -at- 250W 1' on, 1' off. Repeated several times removing the
vacuum to observe the larvae. At first when vacuum was applied the larvae
floated to the top and squirmed around a bit, then after several attempts
at that they finally all stopped moving and didn't float. Buffer washed on
benchtop then used the vacuum again at the same settings for the buffered
osmium tetroxide. Just the ends of the larvae turned black. I tried it
again without any change. I finally got tired of messing with them and
placed them into a KFeCN + OsO4 overnight at 4C and have had them stored in
buffer. ( I have used the overnight KFeCN + OsO4 with success for hard to
infiltrate nematodes). Dehydrated with MW 40s -at- 250W no vacuum 40 s on, 1'
off, infiltrated 50% acetone/Embed no vac at 250W 3', 100% Embed -at- 450W
with vac (20mm Hg). The larvae had areas of collapse after the 100% with
vacuum so I'm concerned that they are not infiltrating as I had hoped.

Karen L. Kelley
ICBR Electron Microscopy Manager
University of Florida
ICBR Electron Microscopy Core Lab
Bartram Hall Room 214
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
Email: klk-at-biotech.ufl.edu
Southeastern Microscopy Society Treasurer
http://www.biotech.ufl.edu/EM/

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 17:18:35 2004

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Dear colleague,
I remember some discussion about CD-R storage. Some CD are not good at all
and in many cases some CD become unreadable after 3 years.
I would like to know what is the best brand or type of CD for storing
images

Keep care and be of good cheer

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

Tenebrionidae of the World, incl. Alleculinae and Lagriinae,
higher taxonomy, Australian beetles.
websites:
http://www.coleoptera.org. and
http://www.egroups.com/group/coleoptera

University of Sydney
The Wentworth Bldg., B 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: ricardo-at-ans.com.au
vratislav-at-bigfoot.com
ICQ: 13610107

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 17:23:42 2004

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We have been using a digital length gauge from Heidenhain. the readout
unit has an RS-232 port so that we can record depth with a computer.
It uses an optical encoder that has been clamped in a holder so that it
can be placed below the stage.

Our cost was about $1500, but that was about 1993.

Regards,
Glen
On Sep 16, 2004, at 9:11 AM, hkonishi-at-indiana.edu wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I am looking for a "micrometer" that can work with optical microscope.
} I think
} that it read the difference of dial for focusing and convert to actual
} distance (um). I want to use it for determining the thickness of disc
} during
} disc grinding if it is not so expensive.
} Please advise on website or product information.
} Thank you,
}
} Hiromi Konishi
} Indiana University
}
}
}
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

************************************************************************
******
The box said "Requires Windows 95 or better", so I bought a Macintosh.
************************************************************************
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From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 17:32:22 2004

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Can you make an estimate how much a high quality print, letter size in full
color and b/w costs (probably just ink and paper)?

I'd be interested also for the HP that was mentioned in the original post.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: Alan Stone [mailto:as-at-astonmet.com]
Sent: Thursday, September 16, 2004 13:36
To: microscopy-at-microscopy.com

We just purchased a Canon i9900. The initial test prints are beautiful. It
uses eight cartridges and when using Canon's Pro Paper, you would not know
that they are computer prints rather than high quality true photographs. We
elected to have a larger format capacity (13" x 19") as we like to decorate
our lab and office walls with our favorites.

Al Stone
ASTON Metallurgical Services

At 01:02 PM 9/16/2004, you wrote:

} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 18:53:41 2004

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IMO, Mitsui is the absolute best for CD-R or
DVD-R:

http://www.mitsuicdr.com/

gary g.

At 03:46 PM 9/16/2004, you wrote:

} Dear colleague,
} I remember some discussion about CD-R storage. Some CD are not good at all
} and in many cases some CD become unreadable after 3 years.
} I would like to know what is the best brand or type of CD for storing
} images
}
} Keep care and be of good cheer
}
} Regards
}
} (name) Vratislav Richard Eugene Maria John Baptist
} (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 20:26:25 2004

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(9/17/04 8:46) Vr.R.E.M.J..-B. BEJSAK-COLLOREDO-MANSFELD {ricardo-at-ans.com.au} wrote:

} Dear colleague,
} I remember some discussion about CD-R storage. Some CD are not good at all
} and in many cases some CD become unreadable after 3 years.
} I would like to know what is the best brand or type of CD for storing
} images
}
} Keep care and be of good cheer
}
} Regards
}
} (name) Vratislav Richard Eugene Maria John Baptist
} (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

I would recommend Mitsui. Remember, the ideal conditions for storage of CDs is very similar to negatives: cool, dark, and dry.

It is essential to remember that a CDR is -absolutely not- an archival copy of your data.

Brent

--
Brent Neal, Ph.D.
Reindeer Graphics, Inc.
brent-at-reindeergraphics.com

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 16 22:43:50 2004

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One thing of note....

DO NOT use sharpie markers or any other acid base pen to label cd's or
dvd's. The acid in these pens etches through the top of the disk destroying
your data.

Dave Crone B.E. (Mechanical)
Engineer-in-Training
Department Assistant
Metallurgical Lab
Mechanical Engineering
College of Engineering
University of Saskatchewan
57 Campus Drive
Saskatoon, SK
S7N 5A9
Phone: (306) 966-5461
Fax: (306) 966-5427
E-mail: dgc132-at-mail.usask.ca
-----Original Message-----
} From: Brent Neal [mailto:brent-at-reindeergraphics.com]
Sent: Thursday, September 16, 2004 7:54 PM
To: Vr.R.E.M.J..-B. BEJSAK-COLLOREDO-MANSFELD
Cc: MICROSCOPY

(9/17/04 8:46) Vr.R.E.M.J..-B. BEJSAK-COLLOREDO-MANSFELD
{ricardo-at-ans.com.au} wrote:

} Dear colleague,
} I remember some discussion about CD-R storage. Some CD are not good at all
} and in many cases some CD become unreadable after 3 years.
} I would like to know what is the best brand or type of CD for storing
} images
}
} Keep care and be of good cheer
}
} Regards
}
} (name) Vratislav Richard Eugene Maria John Baptist
} (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

I would recommend Mitsui. Remember, the ideal conditions for storage of CDs
is very similar to negatives: cool, dark, and dry.

It is essential to remember that a CDR is -absolutely not- an archival copy
of your data.

Brent

--
Brent Neal, Ph.D.
Reindeer Graphics, Inc.
brent-at-reindeergraphics.com

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 01:24:07 2004

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Hi all

We use on an old OPL inverted microscope (~1950) a Mitutoyo digital
workshop micrometer (Digimat IDF 543 serie, ref 543-511) witch gives 1
micron as last digit. It's easy to put in on inverted microscope, a bit
more complicate for a upright one. Very useful and reliable enough for all
samples thinning job for TEM (before tripod polishing or dimpler
thinning). I don't remember the price, we bought it 6-7 years ago, but
it's � workshop stuf, not a laboratory one. So it should not be much
expensive. Some models can be interface to record measurements.

No interest in that manufacturer, just satisfied from that product.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Mat�riaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Thu, 16 Sep 2004 hkonishi-at-indiana.edu wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I am looking for a "micrometer" that can work with optical microscope. I think
} that it read the difference of dial for focusing and convert to actual
} distance (um). I want to use it for determining the thickness of disc during
} disc grinding if it is not so expensive.
} Please advise on website or product information.
} Thank you,
}
} Hiromi Konishi
} Indiana University
}
}

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 06:30:11 2004

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For archival quality:
you should always finalise the disk and don't bother with multisession, consider copying at a slower than maximum speed for the drive and disk. Run the disk check software and maybe check some images on the CD (or just the disk directory) because problems may happen at the copying stage. I have used those special CD markers (I know TDK make some) for about two to three years without problem.

Make 2 (or 3) copies instead of one and store separately and check one periodically - at least if one fails you have a reasonable chance that the other(s) may survive.

Make sure that you will be able to read them in the next few years - with the advance of technologies such as 'Blu-ray' DVD writers how long will CD compatibility be maintained? You only have to look at 8, 5 1/4, & 3 inch floppies and some of the older magneto optical formats to realize that the format may have a shorter useful lifetime than its archival quality.

Malcolm

PS I don't always practice what I preach.

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
} From: Dave Crone {dgc132-at-mail.usask.ca}

So what should be used to archive electronic data?

Richard Doelle
Dofasco Inc.
richard_doelle-at-dofasco.ca

-----Original Message-----
} From: Brent Neal [mailto:brent-at-reindeergraphics.com]
Sent: Thursday, September 16, 2004 9:54 PM
To: Vr.R.E.M.J..-B. BEJSAK-COLLOREDO-MANSFELD
Cc: MICROSCOPY

HI Garry,
I too, mix by weight but I have a friend who uses disposable syringes
to measure by volume. No cleaning, since she uses each syringe once
and discards it (adding to the trash load...we just can't win).
She sets up her resin component bottles with stoppers and tubing that
she can seal off when not measuring and which she can just hook up to
the syringes and then draw out the volume she needs. I know that she
got this method from her first employer and that between them the
method has worked beautifully for 30 years or more.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 08:02:17 2004

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At 4:47 PM -0500 9/16/04, Ying.Shi wrote:
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Ying Shi,

We have had a Hitachi S4700 since 10/1996. We typically only flash
the tip only once in the morning each day. If I remember correctly,
the instrument was originally programmed to make you flash about
every eight hours. We had the service engineer change the required
flash time to 16 hours. This allows us to have a stable beam current
for XEDS line scans and maps in the afternoon. We record the current
at each flash, when this gets above a certain level it indicates to
the service engineer that the column needs to be baked, typically
twice a year. We are still using our original tip!! Good luck with
your S4700.

--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 08:02:08 2004

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Ying;

I think you would be safe in doing as the applications engineer tells
you, i.e. every 48 hrs. Of course, if you have samples that outgas,
this may need to be more frequent. If the emission current is stable,
and the extraction voltage is stable, just let it run.

We have 2 4700's and a 4500 here at Agere, Allentown, PA.

Hope that is of some help.

Peter Tomic
Agere System

-----Original Message-----
} From: Ying.Shi [mailto:Ying.Shi-at-delphi.com]
Sent: Thursday, September 16, 2004 5:48 PM
To: Microscopy-at-MSA.Microscopy.com

Dear All EM Specialists:

I really enjoy being on this forum and am educated a lot. Now I have a
question about how often we need to flash our field emission SEM's tip.

We have a brand new Hitachi 4700 which has a cold field emission tip.
When it was first installed, the service engineer told us to record the
initial extraction voltage each time right after flashing, during the
use if the increase of extraction voltage is more than 1.4 KV, we need
to do a flash. We follow this rule strictly. Several weeks ago Hitachi's
application engineer visited us and said by doing that we flashed too
much and would short our tip's lifetime. We do not need to flash until
we see the tip noise. I never see any tip noise in my image by following
his suggestion so I am forced to flash by the instrument setting (every
48 hours).

Could you please kindly share your experience with me?

Thanks

Ying Shi

Analytic Scientist
Delphi Catalyst
ying.shi-at-delphi.com

************************************************************************
****************

Note: The information contained in this message may be privileged and
confidential and thus protected from disclosure. If the reader of this
message is not the intended recipient, or an employee or agent
responsible for delivering this message to the intended recipient, you
are hereby notified that any dissemination, distribution or copying of
this communication is strictly prohibited. If you have received this
communication in error, please notify us immediately by replying to the
message and deleting it from your computer. Thank you.

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****************

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 08:10:25 2004

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Ying,

We have a Hitachi S4700, too, and were told by our service engineer to
flash about 1.3 KV above the starting extraction voltage. We have had
the same tip since installation in 1999 and since then the starting
extraction voltage (i.e., right after flashing) has increased from 4.1
to 4.3 KV at 5.0 KV accelerating voltage at 10ua beam current.

My impression from this is that the tip is relatively robust and
long-lasting, even under frequent flashing. This included one period of
VERY frequent flashing due to a bad connection to the gun which
prevented flashing from occurring at the proper strength. When this was
discovered and repaired, the first flash was so powerful it gave us a
reading of 72, rather the recommended 20, before we dialed down the
flash intensity! We were afraid that we'd taken the end right off the
tip with this one, but there was no apparent harm and resolution has
remained fine since then.

Whether or not this is better than flashing only when the software tells
you to is unclear to me, but this is how we were told to do it.

Hope this helps.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

-----Original Message-----
} From: Ying.Shi [mailto:Ying.Shi-at-delphi.com]
Sent: Thursday, September 16, 2004 4:48 PM
To: Microscopy-at-MSA.Microscopy.com

Dear All EM Specialists:

I really enjoy being on this forum and am educated a lot. Now I have a
question about how often we need to flash our field emission SEM's tip.

We have a brand new Hitachi 4700 which has a cold field emission tip.
When it was first installed, the service engineer told us to record the
initial extraction voltage each time right after flashing, during the
use if the increase of extraction voltage is more than 1.4 KV, we need
to do a flash. We follow this rule strictly. Several weeks ago Hitachi's
application engineer visited us and said by doing that we flashed too
much and would short our tip's lifetime. We do not need to flash until
we see the tip noise. I never see any tip noise in my image by following
his suggestion so I am forced to flash by the instrument setting (every
48 hours).

Could you please kindly share your experience with me?

Thanks

Ying Shi

Analytic Scientist
Delphi Catalyst
ying.shi-at-delphi.com

************************************************************************
****************

Note: The information contained in this message may be privileged and
confidential and thus protected from disclosure. If the reader of this
message is not the intended recipient, or an employee or agent
responsible for delivering this message to the intended recipient, you
are hereby notified that any dissemination, distribution or copying of
this communication is strictly prohibited. If you have received this
communication in error, please notify us immediately by replying to the
message and deleting it from your computer. Thank you.

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****************

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 08:11:19 2004

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Ying,

We have a Hitachi S-900 field-emission SEM, and I agree with the 2nd
engineer's comment. Flash when you see noise, not by hours or
extraction voltage. If the extraction voltage gets excessive, then
I'd consider flashing, but "excessive" depends on your instrument and
operating variables. 1.4kV may not be "excessive". For us it would be
-- but we rarely seen that much increase in extraction V.

Phil

} Dear All EM Specialists:
}
} I really enjoy being on this forum and am educated a lot. Now I have
} a question about how often we need to flash our field emission SEM's
} tip.
}
} We have a brand new Hitachi 4700 which has a cold field emission
} tip. When it was first installed, the service engineer told us to
} record the initial extraction voltage each time right after
} flashing, during the use if the increase of extraction voltage is
} more than 1.4 KV, we need to do a flash. We follow this rule
} strictly. Several weeks ago Hitachi's application engineer visited
} us and said by doing that we flashed too much and would short our
} tip's lifetime. We do not need to flash until we see the tip noise.
} I never see any tip noise in my image by following his suggestion so
} I am forced to flash by the instrument setting (every 48 hours).
}
} Could you please kindly share your experience with me?
}
} Thanks
}
} Ying Shi
}
} Analytic Scientist
} Delphi Catalyst
} ying.shi-at-delphi.com
}
}
} ****************************************************************************************
}
} Note: The information contained in this message may be privileged
} and confidential and thus protected from disclosure. If the reader
} of this message is not the intended recipient, or an employee or
} agent responsible for delivering this message to the intended
} recipient, you are hereby notified that any dissemination,
} distribution or copying of this communication is strictly
} prohibited. If you have received this communication in error, please
} notify us immediately by replying to the message and deleting it
} from your computer. Thank you.
}
} ****************************************************************************************

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 08:33:55 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bgorman-at-unt.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 16, 2004 at 09:59:31
---------------------------------------------------------------------------

Email: bgorman-at-unt.edu
Name: Brian Gorman

Organization: University of North Texas

Title-Subject: [Microscopy] [Filtered] H-9000 Gun Housing

Question: Dear Listers,

We are dismantling our H-9000 TEM, and have a brand new (never installed) gun housing with retro fit kit available to anyone interested. Please contact me for more details.

If anyone wants other parts of this microsope, please contact me as well.

Regards,
Brian

Brian P. Gorman, PhD
Assistant Professor
Dept. of Materials Science and Engineering
University of North Texas
Denton, TX 76203
bgorman-at-unt.edu
ph: 940-891-6778
fax: 940-565-4824

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 09:10:17 2004

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richard_doelle-at-dofasco.ca wrote:

}
} So what should be used to archive electronic data?
}

I recommend a stylus and clay tablets. Stored under
proper conditions, these have been shown to last
several thousand years.

Rick Mott

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 10:34:55 2004

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Depending on your definition of archival, Mitsui DVD-R
media ought to work for you. Their media is rated at
75 year retention. Their Gold Archival CD-R is rated
at 100 years. Obviously, don't expect these results
if you leave them on the rear deck of your car for
twenty years.

I believe that this topic has been discussed before
and archived. If not, it will get archived now.

My belief and strategy is to have four, redundant backups.
The first backup is either dual CD-R or dual DVD-R.
Second backup is dual hard drives. These are typically
120GB IDE/ATAPI. Third backups are two SNAP/NAS
servers, 340GB-1TB. Fourth backup are two identical
Ultrium 1 or Ultrium 2 LTO tapes.

The hard drives are in removeable bays and are real time
storage and retrieval media. The SNAP/NAS servers are
about the same but are slower due to LAN speed. Once a
directory of data on a drive will fill a CD or DVD, it is written to
the optical media. At this point, the last backup is to
the optical media. The first backup is to hard drive, then
to NAS, then to LTO and finally, optical.

The LTO 1 media will hold 100GB of un-compressed data.
The LTO 2 holds 200GB. Since the image data is either
TIFF or JPEG, it does not really compress much more
at all. LTO is backward compatable to LTO 1. The drives
are wide SCSI and work with most all operating systems.
NovaStore and Veritas make backup software for these
drives.

A most important aspect of backing up is to actually do it.

gary g.

At 06:05 AM 9/17/2004, you wrote:

} So what should be used to archive electronic data?
}
} Richard Doelle
} Dofasco Inc.
} richard_doelle-at-dofasco.ca
}
}
} -----Original Message-----
} } From: Brent Neal [mailto:brent-at-reindeergraphics.com]
} Sent: Thursday, September 16, 2004 9:54 PM
} To: Vr.R.E.M.J..-B. BEJSAK-COLLOREDO-MANSFELD
} Cc: MICROSCOPY
} Subject: [Microscopy] Re: Brand / type of CD-R disk for sImages
}
}
}
}
} I would recommend Mitsui. Remember, the ideal conditions for storage of
} CDs is very similar to negatives: cool, dark, and dry.
}
} It is essential to remember that a CDR is -absolutely not- an archival
} copy of your data.
}
} Brent
}
} --
} Brent Neal, Ph.D.
} Reindeer Graphics, Inc.
} brent-at-reindeergraphics.com

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 10:42:03 2004

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I'm really not sure that I should say this, but for some serious archiving
requirements, one could always convert the digital files to film.

Damian

So what should be used to archive electronic data?

Richard Doelle
Dofasco Inc.
richard_doelle-at-dofasco.ca

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 12:03:50 2004

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For all you aficionados of classic, operating ultramicrotomes---this is
your chance! We are disposing of four LKB Ultratome III's (control and
main units), which, when last assembled, comprised three operating units
plus one for spare parts. Included are numerous separate spare parts,
including chucks, belts, fuses, etc., etc., etc.

We also have a operating Pyramitome 11800 and an LKB Historange 2218
Microtome.

The catch: whoever gets these units is responsible for supplying packing
materials and shipping costs. The machines themselves are free.

We had been using this equipment for all of our ultramicrotomy until the
last couple years and had planned to keep using them as teaching units,
but we are desperately short on space. We hope we can find a good home
for them out there in EM land, because our alternative is surplusing
them with our beat up filing cabinets and broken computer monitors.

Any takers?

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 12:30:56 2004

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On Sep 16, 2004, at 3:46 PM, Vr.R.E.M.J..-B. BEJSAK-COLLOREDO-MANSFELD
wrote:

} I remember some discussion about CD-R storage. Some CD are not good at
} all
} and in many cases some CD become unreadable after 3 years.
} I would like to know what is the best brand or type of CD for storing
} images
}
} Keep care and be of good cheer
}
Dear Vratislav,
We are using Imation disks. Since the cost is small, we get the best
combination of brand name and price. If someone like Computer Shopper
has done a test for the reliability of various brands of CD-R or DVD-R
disks, I'd be interested in the results.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 14:25:05 2004

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Agree on this interest point. I've bought brand-name CD-R that show a rating of "48X Multispeed" and found that my recorder (rated up to 24X) can only record at 4X on these CDs. Curiously, other CD-R from the same manufacturer with nominally the same rating and price can go at the full 24X of my recorder. I asked the fellow at our local computer shop and he gave me the blank stare of ignorance followed by a shoulder shrug.

If anyone can explain how I can determine which CD-R will record "fast" (and as Vratislav suggests, will also have a long life) I'm all ears. I suppose speed ratings on CDs are like mileage ratings on cars - your results may vary from the sticker...

Bill
William A. Heeschen, Ph.D.
Microscopy, Digital Imaging
The Dow Chemical Company
Midland, MI 48674
waheeschen-at-dow.com

-----Original Message-----
} From: Bill Tivol [mailto:tivol-at-caltech.edu]
Sent: Friday, September 17, 2004 2:07 PM
To: microscopy-at-msa.microscopy.com

Wow, there's a big demand for Ultratome III's out there. I think I have
enough takers for now, so early next week I'll sort through the replies
and see what I can do, starting with the first ones.

Correction: The Historange microtome is not included, after all. I
almost gave away my boss's machine by mistake....

Thanks for responding.

Randy

-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Friday, September 17, 2004 1:31 PM
To: microscopy-at-microscopy.com
Cc: Katz, Martin

For all you aficionados of classic, operating ultramicrotomes---this is
your chance! We are disposing of four LKB Ultratome III's (control and
main units), which, when last assembled, comprised three operating units
plus one for spare parts. Included are numerous separate spare parts,
including chucks, belts, fuses, etc., etc., etc.

We also have a operating Pyramitome 11800 and an LKB Historange 2218
Microtome.

The catch: whoever gets these units is responsible for supplying packing
materials and shipping costs. The machines themselves are free.

We had been using this equipment for all of our ultramicrotomy until the
last couple years and had planned to keep using them as teaching units,
but we are desperately short on space. We hope we can find a good home
for them out there in EM land, because our alternative is surplusing
them with our beat up filing cabinets and broken computer monitors.

Any takers?

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 17:39:54 2004

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I don't think the terms "Archival" and CD-R/DVD-R technologies are
compatible and should not be used in the same sentence unless associated
with the phrase "do not count on".

Yes, manufacturers will offer ratings of 75 or 100 years.

If the media is not exposed to _ANY_ variations in temperature,
humidity, etc. and never handled, I expect you could see that life. I
know of very few places on earth that meet those requirements.

There are a number of potential things that will render the media
unusable. This includes not only the recordable, but also the
"pre-recorded" or "permanent" versions.

The permanent versions especially are susceptible to the aluminum layer
(the LABEL side) oxidizing, and then falling apart.

As the disks go through thermal cycling, there can also be problems with
de-lamination.

Scratches on the "bottom" side of the media are relatively harmless.
Some media is VERY susceptible to scratching and damage on the top or
label side. In addition to avoiding the acidic "Sharpies", avoid
pencils, ball point pens, etc. I would also caution against trying to
"undo" any pressure sensitive label.

DVD-R may prove to be more stable then their CD-R counterparts. I would
still be cautious. Many of the vulnerabilities of CDs (permanent and
recordable) were not realized until recently. There may be unknown
vulnerabilities to DVD-Rs that we don't know about yet either.

Probably the most reliable "archive" media is still magnetic tape. I
have know people that have taken a tape from a "fireproof" safe, where
the tape cartridge was melted beyond use (due to longer than rated
exposure), and even appeared "melted into" the tape. The tape was sent
out to a recovery specialist and 90% plus of the data was recovered.
The stuff takes a beating and keeps on winding.

John W. Raffensperger, Jr.
IT Manager
Apache Stainless

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 17:55:04 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bwadgaobkar-at-mail.mcg.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, September 17, 2004 at 13:15:12
---------------------------------------------------------------------------

Email: bwadgaobkar-at-mail.mcg.edu
Name: Bakul wadgaonkar

Organization: Medical College of Georgia

Education: Graduate College

Location: Augusta, GA

Question: We are studying a polymer (poly e-caprolactone)thats semicrystelline. We were wondering how to stain just the crystelline lamella to eatimate the crystillinity and study the morphology.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 19:45:34 2004

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I concur with what all that was said. But still cd's are cheap, 25 cents a
piece here, and at 40x or faster you are looking at 5 to 6 min burn. A
non-acidic marker is $2, so backup your data and if you are unsure back it
up again in 6 months. If it is dvd-r they are $0.46 and you can fit 4.2 GB
on them, so back up often! If you have problems with data backup feel free
to contact me I will help you out as much as I can.

Dave Crone B.E. (Mechanical)
Engineer-in-Training
Department Assistant
Metallurgical Lab
Mechanical Engineering
College of Engineering
University of Saskatchewan
57 Campus Drive
Saskatoon, SK
S7N 5A9
Phone: (306) 966-5461
Fax: (306) 966-5427
E-mail: dgc132-at-mail.usask.ca
-----Original Message-----
} From: Chiphead [mailto:chiphead-at-sbcglobal.net]
Sent: Friday, September 17, 2004 5:07 PM
To: 'Microscopy MSA'

I don't think the terms "Archival" and CD-R/DVD-R technologies are
compatible and should not be used in the same sentence unless associated
with the phrase "do not count on".

Yes, manufacturers will offer ratings of 75 or 100 years.

If the media is not exposed to _ANY_ variations in temperature,
humidity, etc. and never handled, I expect you could see that life. I
know of very few places on earth that meet those requirements.

There are a number of potential things that will render the media
unusable. This includes not only the recordable, but also the
"pre-recorded" or "permanent" versions.

The permanent versions especially are susceptible to the aluminum layer
(the LABEL side) oxidizing, and then falling apart.

As the disks go through thermal cycling, there can also be problems with
de-lamination.

Scratches on the "bottom" side of the media are relatively harmless.
Some media is VERY susceptible to scratching and damage on the top or
label side. In addition to avoiding the acidic "Sharpies", avoid
pencils, ball point pens, etc. I would also caution against trying to
"undo" any pressure sensitive label.

DVD-R may prove to be more stable then their CD-R counterparts. I would
still be cautious. Many of the vulnerabilities of CDs (permanent and
recordable) were not realized until recently. There may be unknown
vulnerabilities to DVD-Rs that we don't know about yet either.

Probably the most reliable "archive" media is still magnetic tape. I
have know people that have taken a tape from a "fireproof" safe, where
the tape cartridge was melted beyond use (due to longer than rated
exposure), and even appeared "melted into" the tape. The tape was sent
out to a recovery specialist and 90% plus of the data was recovered.
The stuff takes a beating and keeps on winding.

John W. Raffensperger, Jr.
IT Manager
Apache Stainless

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 20:18:09 2004

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(9/17/04 9:01) Gary Gaugler {gary-at-gaugler.com} wrote:

} Depending on your definition of archival, Mitsui DVD-R
} media ought to work for you. Their media is rated at
} 75 year retention. Their Gold Archival CD-R is rated
} at 100 years. Obviously, don't expect these results
} if you leave them on the rear deck of your car for
} twenty years.
}
} I believe that this topic has been discussed before
} and archived. If not, it will get archived now.
}
} My belief and strategy is to have four, redundant backups.
} The first backup is either dual CD-R or dual DVD-R.

}
} The hard drives are in removeable bays and are real time
} storage and retrieval media. The SNAP/NAS servers are
\
}
} The LTO 1 media will hold 100GB of un-compressed data.

}
} A most important aspect of backing up is to actually do it.
}
} gary g.

Of course, in 20 years, no drives will exists that will read any of the above media, so it really doesn't matter how long the supposed "archival" DVDs last, now does it. How many DECtape readers have -you- seen recently?

Brent

--
Brent Neal, Ph.D.
Reindeer Graphics, Inc.
brent-at-reindeergraphics.com

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 17 23:55:33 2004

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} From: "Brent Neal" {brent-at-reindeergraphics.com}
:
: (9/17/04 9:01) Gary Gaugler {gary-at-gaugler.com} wrote:
:
: } Depending on your definition of archival, Mitsui DVD-R
: } media ought to work for you. Their media is rated at
: } 75 year retention. Their Gold Archival CD-R is rated
: } at 100 years. Obviously, don't expect these results
: } if you leave them on the rear deck of your car for
: } twenty years.
: }
: } I believe that this topic has been discussed before
: } and archived. If not, it will get archived now.
: }
: } My belief and strategy is to have four, redundant backups.
: } The first backup is either dual CD-R or dual DVD-R.
:
: }
: } The hard drives are in removeable bays and are real time
: } storage and retrieval media. The SNAP/NAS servers are
: \
: }
: } The LTO 1 media will hold 100GB of un-compressed data.
:
: }
: } A most important aspect of backing up is to actually do it.
: }
: } gary g.
:
:
:
: Of course, in 20 years, no drives will exists that will read any
of the above media, so it really doesn't matter how long the
supposed "archival" DVDs last, now does it. How many DECtape readers
have -you- seen recently?
:
If you want to preserve your work, data, photos, notes and such
forever there is no computer media up to the task. Only a system
that relies on copies of the data sorted in geographically diverse
location that are recopied to new media and updated media as it
becomes available. This quickly becomes a very large difficult
problem much like putting one grain of wheat on the first square of
a chess board, 2 on the second, 4 on the third, ..... and you exceed
the world capacity to grow wheat before the chess board is full. It
is more practical with computer data but not a lot and any of us the
are truly confident of the integrity our back ups are few and far
between.

I still contend that black and white silver halide film is the least
expensive longest lived option available. We can all do it and
afford it and the technology is well tested an proven. Building a
device to put digital information on 35 mm film would be trivial and
we all have the equipment for our images. Adding a copy stand covers
notes and printed matter. With today's technology that we can use
that is proven to have a 150 year life stored in a shoe box. Modern
film substrates probably fixed and toned stored in a controlled
atmosphere should last a very long time indeed.

Digital images, data and writing are sure a lot more convenient but
they are sure not as reliable or as long lived as conventional
photography.

Gordon
Gordon Couger gcc-at-couger.com

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward any links or information you think might be useful to
others.
Microscope Manual at www.science-info.org

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 18 07:40:53 2004

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Gordon Couger writes ...

} : Of course, in 20 years, no drives will exists that will read any
} of the above media, so it really doesn't matter how long the
} supposed "archival" DVDs last, now does it. How many DECtape readers
} have -you- seen recently?

I don't know that I'd agree. Today's optical media and drives appear to
offer a means for total backward compatibility ... e.g., DVD drive which can
read CDs. This is of course up to the manufacturers sticking to it and
supporting its application toward archiving.

That said ... I believe this thread should be as much about the
dependability of the software. It isn't so much about the media ... e.g.,
if an archive was written today, what software would be able to restore it
in 20 years? What can I use with confidence? ... which will write archives
identical to the original directory structure, without splitting files, and
verify the archive integrity??? Will the software maintain any database
characteristics of the original collection of files?? What software would
support and manage redundant archives across multiple devices (as Gary
Gaugler suggested as required)??

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 18 08:29:08 2004

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Perhaps we're way too obsessed with saving everything.
-Michael

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 18 08:43:29 2004

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(9/18/04 9:54) Michael Cammer {cammer-at-aecom.yu.edu} wrote:

} Perhaps we're way too obsessed with saving everything.
} -Michael

You try doing a patent defense 15 years later without the original micrographs.

B
--
Brent Neal, Ph.D.
Reindeer Graphics, Inc.
brent-at-reindeergraphics.com

From MicroscopyL-request-at-ns.microscopy.com Sat Sep 18 10:34:43 2004

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I have a DEC TK-70, circa 1990. I don't have
any VAX systems any more so the drive is not
really being used. That said, it is a DLT-II
tape unit. This format is still offered, and
media is still available. It is not a huge
repository of data. So from a hardware standpoint,
this is a viable option. But the point of selecting
a long lived platform is valid.

The longer the media type and drives stay around,
it is all that much longer before the data needs to
be migrated to newer methods. If one picks reliable
and solid backup software from either NovaStore or
Veritas, they very much seem to be fully backwards
compatible regardless of the OS that was used to
host the app. Furthermore, these apps will backup
to drives, tapes, CDs and DVDs. So, it gets back
to the hardware media set. Once written, the app's
format does not change. The new app will have new
features but it allows reading older media that used
earlier versions of the app.

The most long lasting standard as I recall is SCSI.
If the drive works, it can still be read today.
SCSI has evolved to SCSI-II and more exotic types
like LVD (low voltage differential). Even Adaptec
LVD adapters will work with single ended narrow SCSI
devices. They have made dual channel adapters so
each side can work with either type of media.

SCSI is fast but it is costly. Hence, this is why
I prefer the IDE/ATAPI drives at about 1/3 the cost
of a similar SCSI drive. MFM was around at first
but gave way to IDE, and IDE is still here. What is
happening now is that the parallel IDE/ATAPI is
evolving to serial ATA (SATA). They are not plug
compatible. Even if mother boards drop parallel
IDE, there are SATA-to-IDE adapters. In fact, current
versions of SATA drives include a SATA-to-IDE adapter
since true SATA drives are just now coming to market.

My selection of backup was carefully made to support
archiving tens of thousands of digital images. Many
of these are 30MB-250MB TIFF each. As was pointed out,
technology moves on. Consequently, a one time archive
is not going to last indefinitely into the future.
But it potentially could if properly designed. I'm
not taking that chance.

How many specimens do you all prepare and archive? What
about this facet of preservation? If the captured
data was lost, having the specimen would allow re-doing
the imaging. A pain for sure but not fatal. Properly
prepared specimens stored in a vacuum seem to hold up
for a long, long time with no discernable effects.
So, archive the specimens (this can get overwhelming
for sure) and archive the image results.

BTW, I use a fine point Sharpie to write on CDs and DVDs.
I never have a problem. The big felt markers are a no-no.

gary g.

At 06:08 AM 9/18/2004, you wrote:

} Gordon Couger writes ...
}
} } : Of course, in 20 years, no drives will exists that will read any
} } of the above media, so it really doesn't matter how long the
} } supposed "archival" DVDs last, now does it. How many DECtape readers
} } have -you- seen recently?
}
} I don't know that I'd agree. Today's optical media and drives appear to
} offer a means for total backward compatibility ... e.g., DVD drive which can
} read CDs. This is of course up to the manufacturers sticking to it and
} supporting its application toward archiving.
}
} That said ... I believe this thread should be as much about the
} dependability of the software. It isn't so much about the media ... e.g.,
} if an archive was written today, what software would be able to restore it
} in 20 years? What can I use with confidence? ... which will write archives
} identical to the original directory structure, without splitting files, and
} verify the archive integrity??? Will the software maintain any database
} characteristics of the original collection of files?? What software would
} support and manage redundant archives across multiple devices (as Gary
} Gaugler suggested as required)??
}
} cheerios ... shAf :o)

From MicroscopyL-request-at-ns.microscopy.com Sun Sep 19 12:03:15 2004

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Hi,

A nice introduction to astigmatism in microscopy can be found at:

http://micro.magnet.fsu.edu/primer/java/aberrations/astigmatism/

Autofocusing on a digital microscope works fine, even at high
magnification (63x, 1.4 NA) in both brightfield as well as fluorecence
microscopy, as was published in:

Geusebroek J.M., Cornelissen F., Smeulders A. W. M., and Geerts H.,
Robust autofocusing in microscopy.
Cytometry, 36(1), pp. 1-9, (2000).

Regardless of which algortihm you use, it is important to sample your
Z-stack at the appropriate interval (see Geusebroek et al.).

Regards,

Peter

----------------------------------------------
Peter Van Osta

Director Imaging
MAIA SCIENTIFIC (formerly Union Biometrica NV)
Cipalstraat 3
B-2440 Geel, Belgium
Tel.: +32 (0)14 570 620
Mobile: +32 (0)497 228 725
Fax.: +32 (0)14 570 621
Email: pvosta-at-maia-scientific.com
Website: www.maia-scientific.com
A Harvard Bioscience Company
----------------------------------------------

by way of MicroscopyListserver wrote:
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} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kssim-at-mmu.edu.my) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday, September 19, 2004 at 11:37:16
} ---------------------------------------------------------------------------
}
} Email: kssim-at-mmu.edu.my
} Name: kssim
}
} Organization: mmu
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Does anyone know tell me whether there is stigmatism problem or auto focusing problem at high resolution on optical misroscope? How to solve this problem?? Let me know ...
}
} Many thanks
} Ks
}

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 20 08:06:05 2004

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Peter Van Osta provided a reference to a very helpful article. Sadly, there was a typo in the reference. The correct citation is

Geusebroek J.M., Cornelissen F., Smeulders A. W. M., and Geerts H.,
Robust autofocusing in microscopy.
Cytometry, 39(1), pp. 1-9, (2000).

The article is worth the look...

I also found the following article helpful:
A. Santos et. al., "Evaluation of autofocus functions in molecular
cytogenic analysis," J. Microsc., 188(3), 264-272 (1997)

In particular, I found the functions "F4" and "F5", attributed to Vollath,
to be robust.

Hope this helps.

John Minter

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 20 17:13:11 2004

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That's a good point about the software needed to access these archived
CD's remaining available. I now have a number of CD's of data that were
made using Roxio's "Direct CD" that I do not have on my new computer.
And Win XP's CD handler won't access the files. The data isn't so
important for me to take the trouble to find Direct CD and load it onto
my new computer just to off load data from old CD's. But it made me
aware of the fact that Win XP's CD handler isn't going to be around
forever either. When the new "Big Thing" shows up, we need to be ready
to transfer anything that's important to the new media. Come to think of
it, I have a bunch of stuff on Jazz cartridges too.

Joiner Cartwright, Jr., Ph.D.
Baylor College of Medicine
Houston, Texas U.S.A.

-----Original Message-----
} From: michael shaffer [mailto:michael-at-shaffer.net]
Sent: Saturday, September 18, 2004 7:09 AM
To: MSA listserver

Gordon Couger writes ...

} : Of course, in 20 years, no drives will exists that will read any of
} the above media, so it really doesn't matter how long the supposed
} "archival" DVDs last, now does it. How many DECtape readers have -you-

} seen recently?

I don't know that I'd agree. Today's optical media and drives appear
to offer a means for total backward compatibility ... e.g., DVD drive
which can read CDs. This is of course up to the manufacturers sticking
to it and supporting its application toward archiving.

That said ... I believe this thread should be as much about the
dependability of the software. It isn't so much about the media ...
e.g., if an archive was written today, what software would be able to
restore it in 20 years? What can I use with confidence? ... which will
write archives identical to the original directory structure, without
splitting files, and verify the archive integrity??? Will the software
maintain any database characteristics of the original collection of
files?? What software would support and manage redundant archives
across multiple devices (as Gary Gaugler suggested as required)??

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 20 19:22:33 2004

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,

Previously I worked for a GLP accredited CRO, and we had some guidelines
on using CDs for backups. At that time I was the Systems Administrator
(Bask in Zoology, go figure...) and wrote most of their Standard
Operating Procedures. Generally this can be summed up:

- Buy gold or silver CD-Rs, and buy a reputable brand. 'Gold' or
'silver' CDs usually use a reactive metal coating to store data, rather
than a photoreactive dye as seen in 'green' or 'blue' CDs. The metal
based CD-R is more stable and (usually) better quality. I'd trust these
for 5 years. Good brands are Kodak, Imation, Verbatium, TDK, Sony,
Mitsubishi. Other brands may be good too, if you trust their VHS or
Audio tapes then you can trust their CD-Rs.

- Never ever CR-RW. Untrustworthy and expensive. CD-Rs are cheap, use
it, store it, throw it away. Better than losing data.

- Buy a good CD writer. This is hard for those not savvy with PCs. Buy a
good brand, and not all the good brands are well-known brands. Asus,
Sony, Pioneer, and Plextor are considered the best. Never use HP CD
writers, though this is mostly from poor personal experience. Finally,
buy a new one every two years, or more often if you use it lots. Make
sure that it has a 2MB buffer or bigger (8MB is best) and some form of
'underrun protection'.

- Get good software and use it. Forget the widgets built into Windows
2000/XP, too easy to stuff up. Don't use DirectCD. Don't use ROXIO (some
versions of Roxio software had some serious bugs that put your whole
hard drive at risk! I'm not forgiving enough to start using their
software again). I recommend Nero Burning ROM. It's cheap, easy, and
often comes bundled with a CD-Writer. CloneCD is a good package for just
copying CDs.

- Keep your CDs safe. A cool dry dark place. A cupboard is fine. Do not
keep CDs in the fridge or freezer, some brands get very brittle at low
temperatures and will shatter in the CD drive. Keep them out of direct
sunlight and don't store them in a car.

Finally for the really paranoid. Don't use CDs, use tapes, or even
better arrange for a reputable archiving company to do your backups for
you and store them off site. If you have to use CDs, make multiple
copies and keep one set off site. Use more than one brand of CD-R and
alternate, so if one brand packs it in, the next month/week CD will
still be Ok.

More will probably come to me later.

Aaron Hicks
Electron Microscopy Preparation Technician

Comparative Physiology and Anatomy
Institute of Veterinary, Animal, and Biomedical Sciences
Massey University

PN-412
Private Bag 11 222
Palmerston North
New Zealand

Phone +64 06 350 4470

At 03:46 PM 9/16/2004, you wrote:

} Dear colleague,
} I remember some discussion about CD-R storage. Some CD are not good at
} all and in many cases some CD become unreadable after 3 years.
} I would like to know what is the best brand or type of CD for storing

} images
}
} Keep care and be of good cheer
}
} Regards
}
} (name) Vratislav Richard Eugene Maria John Baptist
} (surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 20 22:33:34 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (patljg-at-gwumc.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 20, 2004 at 11:47:56
---------------------------------------------------------------------------

Email: patljg-at-gwumc.edu
Name: lesley graham

Organization: george washington university

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Recently, I have been having problems with my thick sections lying flat. Nothing has been changed in the protocol; section, stain, and rinse. No matter how carefully I place the section on the slide, it will not be flat after the slide has dried.

Lesley Graham

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 20 22:33:58 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jameen-at-rohmhaas.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 20, 2004 at 16:05:08
---------------------------------------------------------------------------

Email: jameen-at-rohmhaas.com
Name: Joseph G. Ameen

Organization: Rohm and Haas Electronic Materials

Title-Subject: [Microscopy] [Filtered] MListserver: electronic Magnetic Cancellation Systems

Question: Does any one know if the Electromagnetic Compensation Systems or Magnetic Active Compensation systems work on an SEM? Our company is about to purchase a new SEM and the site survey showed AC fields in the 11 - 29 milliGauss range and DC fields in the 2 mG range. Vibration testing was fine. The stray fields are at least an order of magnitude higher then the recommended levels. Has anyone had experience with these cancellation systems?

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 20 22:34:16 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cmeyer911-at-yahoo.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 20, 2004 at 22:23:28
---------------------------------------------------------------------------

Email: cmeyer911-at-yahoo.com
Name: Chris Meyer

Organization: Boeing

Title-Subject: [Microscopy] [Filtered] [TEM] Selected Area Diffraction ZA Deconvolution

Question: I would like to hear any suggestions as to what is the best free software or web-based solutions site, for de-convoluting zone axis for selected-area diffraction patterns based on angles and length ratios. I'm specifically looking for some help beyond the FCC, BCC and HCP (with Ti c/a ratio)that I find in textbooks.

Any suggestions?

Thank you

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 11:12:26 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

RE: Plastic Membrane Boxes, Gel Sticky Box, Wafer Containers (see website
below)

I am looking for containers of ion-milling samples and small pieces in
progress of sample preparation. I found three types of containers: Membrance
Box, Stickey gel box, and just small containers. Is there any suggestion,
regarding advantage or disadvantage. I am using this when I trip by car or
airplane.

I know that some people use TEM grid boxes that come with carbon films when
you buy it. I don't like this because sometimes I break ion-thinned samples
when I close the cover.

Thank you,

Hiromi Konishi
Indiana University

Products websites:

http://www.mtixtl.com/index.asp?PageAction=VIEWCATS&Category=212

http://www.tedpella.com/box_html/membox.htm

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 11:23:55 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lesley,
I presume that you have already stained your semi-thin sections at that
point, when you notice that the section is no longer flat. One thing that I
have noticed, is that a person needs to wait a bit longer after the section
has dried on the hot plate before they start staining. So when the section
is drying, I am busy typing my slide labels, but even then, when the slide
looks dry and ready to go, it isn't in fact ready to go, and you need to
give it a few more minutes, otherwise you won't have a good bond of the
section to the slide, and it will start to lift off during the staining, and
especially the washing after the staining. This could be the most
frustrating thing in the universe if this happens.

If on the other hand, you give your slide some time to BOND to the slide, (a
few minutes) even after it has dried down, then the sections will refrain
from lifting up and folding over during the staining process.

If you allow it this extra time, you can be quite rough with the slide
during the staining and washing, and those sections won't lift off and fold.
And not only that, but you won't have to use coated slides to make sure that
the sections won't wash off, because they won't be going anywhere.

Garry

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (patljg-at-gwumc.edu) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Monday, September 20, 2004 at 11:47:56
---------------------------------------------------------------------------

Email: patljg-at-gwumc.edu
Name: lesley graham

Organization: george washington university

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Recently, I have been having problems with my thick sections
lying flat. Nothing has been changed in the protocol; section, stain, and
rinse. No matter how carefully I place the section on the slide, it will
not be flat after the slide has dried.

Lesley Graham

---------------------------------------------------------------------------

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 13:11:54 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I have some Kodak EM film SO-163 and the expiration date was July 2003.
It has been stored in the freezer. What are the odds that it is still
useable? Any advice would be appreciated.
thanks in advance,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 13:28:48 2004

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Hello Lesley,
When I cut resin thick sections (0.5 - 5.0 microns) I transfer the
sections to a drop of water on a slide and set the slide on a slide warmer
under a large Petri dish cover with a cotton swab dipped in toluene. The
toluene vapor softens and smooths the resin sections so that they flatten
and adhere to the slide.

At 10:32 PM 9/20/2004 -0500, you wrote:
} Email: patljg-at-gwumc.edu
} Name: lesley graham
} Organization: george washington university
} Title-Subject: [Microscopy] [Filtered] MListserver:
} Question: Recently, I have been having problems with my thick sections
} lying flat. Nothing has been changed in the protocol; section, stain, and
} rinse. No matter how carefully I place the section on the slide, it will
} not be flat after the slide has dried.
} Lesley Graham

Dean Abel
Biological Sciences 143 BB
University of Iowa
Iowa City IA 52242-1324

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 14:55:49 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Definitely useable
Chris

Dr. Chris Jeffree
University of Edinburgh

----- Original Message -----
} From: "Beth Richardson" {beth-at-plantbio.uga.edu}
To: "microscopy microscopy" {microscopy-at-msa.microscopy.com}
Sent: Tuesday, September 21, 2004 7:10 PM

On Sep 21, 2004, at 11:10 AM, Beth Richardson wrote:

} I have some Kodak EM film SO-163 and the expiration date was July
} 2003. It has been stored in the freezer. What are the odds that it is
} still useable? Any advice would be appreciated.
} thanks in advance,

Dear Beth,
When I was at Albany, we had both 4489 and LoDose films that had been
refrigerated for some years when I got there, so was from the 70s or
before. When I used either film and developed it according to
instructions, there was no loss of contrast compared to fresh film, and
the fog level--especially with the LoDose, which is about 1.5 orders of
magnitude more sensitive than SO163--was not measurable; i.e., one
could detect only a few grains in a 10 x 10 um field, and the
microdensitometer reading was 0.000 OD. Unless the quality control has
gotten much worse, I expect that the SO163 should be indistinguishable
in response from brand new film.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 16:19:06 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Hiromi,
I use flat silicon rubber plates to hold ion-milled samples. These are flat
slabs of silicon rubber that fit in a petri plate and the pieces or grids
stick to them, then release them cleanly. They are available from Canemco
(Canada) and probably other EM suppliers and are marked off in numbered
squares. I have also made my own out of the castable silicon rubber kits.
Good luck,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: {hkonishi-at-indiana.edu}
To: {Microscopy-at-microscopy.com}
Sent: Tuesday, September 21, 2004 9:10 AM

Hiromi: I like the membrane boxes for thin, non-mechanically strong
things. They hold tightly but when you take the lid off, the parts can
be handled w/ ease. I love the sticky-gel-type boxes for most
everything else but things do STICK in them and can be damaged when
trying to remove them. For what you're doing, I recommend the membrane
boxes.

hkonishi-at-indiana.edu wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 17:46:49 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (donaldawbrey-at-texashealth.org) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, September 21, 2004 at 09:05:20
---------------------------------------------------------------------------

Email: donaldawbrey-at-texashealth.org
Name: Donald G. Awbrey HT(ASCP) QIHC

Organization: Harrris Methodist Hospital

Title-Subject: [Microscopy] [Filtered] Printers for diagnostic quality images.

Question: Dear fellow mico netters,

We are checking into purchasing a monochrome laser printer that will be used to print EM photos for diagnostic purposes.

The ones we are looking at are at 1200 X 1200 dpi and have a 16-32MB expanded memory. We are not neccessarily interested in print speed.

We will be using a 2K camera on our TEM.

Will this printer give us the quality for photographs that will be used for pathology diagnosis in a clinical setting? Will these prints be of good quality to pass any laboratory inspection (i.e. CAP)?

Thank you in advance for any info.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 18:14:48 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Karen,
There was some discussion of microwave protocols for insect larvae in January 2004. Try the Listserver archives for these. Myself, I found that much trial and error was necessary. Some things you may want to try include:

- Karnovsky's fixative (glutaraldehyde & paraformaldehyde in phosphate buffer)
- injecting the fixative into the specimen
- using the vacuum (I draw the vacuum to 20" Hg and leave it for 5 minutes before MWing. MW with vacuum on.)

Using cooled Karnovsky's, I draw up a vacuum, leave it for 5 min and then MW with an initial sequence at 100 W (2 min 0% power, 2 min 100% power, 3 min 0% power), cool my specimen to 20 deg C, draw up a vacuum and MW for a second sequence at 450 W (30 sec 0% power, 30 sec 100% power, 30 sec 0% power). I repeat the 450 W sequence 3 times. After I let my specimens sit in fix for 5 min.

For my osmium step, with a vacuum and cooled solution, I MW at 100 W for a sequence of 2 min 0% power, 2 min 100% power, 3 min 0% power. I also let the samples sit in osmium for a few minutes (10-20 min).

I've used this on larvae larger than mosquitos (specifically, diamond back moth and Colorado potato beetle) and it has yielded good results. I've also found that processing several samples will yield some with good quality fixation and the others can be discarded.

If you have any questions, feel free to contact me. I'd be interested in hearing how things worked out for you.

Shannan

Shannan Little
Research Technician/Technicien de recherche
Electron Microscopy and Image Analysis /Microscopie �lectronique et Analyse d'images
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/T�l�phone: 403-317-3446
Facsimile/T�l�copieur: 403-382-3156
P.O. Box 3000 / CP 3000
Lethbridge, Alberta T1J 4B1
littlesm-at-agr.gc.ca
http://res2.agr.ca/lethbridge/emia/index_e.htm

-----Original Message-----
} From: Karen Kelley [mailto:klk-at-biotech.ufl.edu]
Sent: Thursday, September 16, 2004 4:06 PM
To: Microscopy-at-MSA.Microscopy.Com

Hello All,

I have a BioScan Ted Pella microwave with a cold-spot and vacuum chamber
that I would like to use for fixation and embedding insect eggs and larvae
for TEM.

The project includes mosquito larvae, pupae and fly eggs. I worked on this
same project many years ago using traditional benchtop methods. The eggs
and larvae were difficult, if not impossible, to infiltrate. Now that I
have a MW with vacuum I am trying this project again.

I would greatly appreciate any suggestions, advice or direct me to references?

My first attempt at MW didn't go so well.
I MW fixed the larvae with 2% glut in cacodylate in 1.5ml centrifuge tubes
at 20mmHg vacuum -at- 250W 1' on, 1' off. Repeated several times removing the
vacuum to observe the larvae. At first when vacuum was applied the larvae
floated to the top and squirmed around a bit, then after several attempts
at that they finally all stopped moving and didn't float. Buffer washed on
benchtop then used the vacuum again at the same settings for the buffered
osmium tetroxide. Just the ends of the larvae turned black. I tried it
again without any change. I finally got tired of messing with them and
placed them into a KFeCN + OsO4 overnight at 4C and have had them stored in
buffer. ( I have used the overnight KFeCN + OsO4 with success for hard to
infiltrate nematodes). Dehydrated with MW 40s -at- 250W no vacuum 40 s on, 1'
off, infiltrated 50% acetone/Embed no vac at 250W 3', 100% Embed -at- 450W
with vac (20mm Hg). The larvae had areas of collapse after the 100% with
vacuum so I'm concerned that they are not infiltrating as I had hoped.

Karen L. Kelley
ICBR Electron Microscopy Manager
University of Florida
ICBR Electron Microscopy Core Lab
Bartram Hall Room 214
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
Email: klk-at-biotech.ufl.edu
Southeastern Microscopy Society Treasurer
http://www.biotech.ufl.edu/EM/

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 19:12:31 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I also swear by the membrane boxes. I use the small 1 x 1 size. For very brittle samples, such as II-Vi and III-V compounds, I don't put the membrane boxes together with full force. Just tight enough so that they can't go from edge to edge. I also re-use them. I use 1" white Post-it tape and tear off long enough to use as a label.

One thing that you must be very careful with using these and that is that the sample must be thoroughly dried from any solvents that you may have used, especially acetone. If the sample is even slightly damp when you put it in, it will stick to the membrane.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)

-----Original Message-----
} From: hkonishi-at-indiana.edu [mailto:hkonishi-at-indiana.edu]
Sent: Tuesday, September 21, 2004 12:11 PM
To: Microscopy-at-microscopy.com

RE: Plastic Membrane Boxes, Gel Sticky Box, Wafer Containers (see website
below)

I am looking for containers of ion-milling samples and small pieces in
progress of sample preparation. I found three types of containers: Membrance
Box, Stickey gel box, and just small containers. Is there any suggestion,
regarding advantage or disadvantage. I am using this when I trip by car or
airplane.

I know that some people use TEM grid boxes that come with carbon films when
you buy it. I don't like this because sometimes I break ion-thinned samples
when I close the cover.

Thank you,

Hiromi Konishi
Indiana University

Products websites:

http://www.mtixtl.com/index.asp?PageAction=VIEWCATS&Category=212

http://www.tedpella.com/box_html/membox.htm

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 21 21:29:25 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All:

When we use our TEM at low magnification (below 4,000X), we sometimes
get a bright spot in the middle of pictures we take.�I always thought
the microscope setting was the only thing responsible for this
problem.�But when I checked into a latest episode, I found pictures
from one particular specimen (thin sections on Formvar coated grid) had
it, but others from a different specimen (negative staining on
Farmvar/carbon coated grid) did not, even though all pictures were
taken with the same scope setting.��Has anyone else experienced the
same?�Can anyone explain to me thoroughly how this problem occurs, and
how to avoid it.�Thank you very much in advance.�

Hong
Emory EM

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 22 07:45:41 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dieter-at-genetik.uni-bielefeld.de) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 22, 2004 at 06:14:11
---------------------------------------------------------------------------

Email: dieter-at-genetik.uni-bielefeld.de
Name: Dieter Kapp

Organization: Academic/University

Title-Subject: [Microscopy] [Filtered] MListserver: Which Deconvolution Software?

Question: Hello Listers!

We are currently searching for a deconvolution software.
Mainly we look at GFP-fusions in single cells, monolayers (Sf9) or cell aggregates (tobacco BY2), up to 100 �m on a Olympus IX81.

What are your experiences with such a software tool?
How are the results compared to confocal images of the same specimen (...okay, deconvolution can be applied to confocal images too)?
Is it worth the money?

The two major competitors in the market are SVI/Huygens and AutoQuant. Which product did you choose and why?
Is there a side by side test of both softwares available? Do they differ in image quality?
We only need deconvolution, since our imaging software is doing well.
Do you have other recommendations/suggestions?

Any comment on this issue will be warmly welcomed!
Thank you in advance,

Dieter

------------------------------------------------------------
Dr. Dieter Kapp
Lehrstuhl fuer Genetik
Postfach 100131
Universitaet Bielefeld PHONE: +49 (0)521 106 5620
D-33501 Bielefeld FAX: +49 (0)521 106 5626
GERMANY Email: dieter-at-genetik.uni-bielefeld.de

------------------------------------------------------------

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From MicroscopyL-request-at-ns.microscopy.com Wed Sep 22 10:19:59 2004

Contents Retrieved from Microscopy Listserver Archives
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Pacific Northwest Microscopy Society (PNMS) announces its annual meeting
held this year in Hillsboro, OR during two half-day sessions on
September 30 and October 1st.

The FEI company generously offered its new facility for the meeting
location, and largely contributed to its program.
Two parallel platform sessions will run in biological and material
sciences. Besides the scientific program, demonstrations of the FIB and
a Technai TEM will be available, as well as the FEI factory tour. The
evening social at the historical Cornelius House and Brewery will
include a barbecue dinner, and it is covered with the registration. The
second day morning biological session will take place in the OSHU
Primate Center including its tour.
Due to the space limitation (50 people), we require advanced
registration. Registration is free of charge to the current PNMS
members, and $10 for non-members.

Program:

Fall 2004 Annual Meeting
When: Thursday Sept. 30th, and Friday October 1st, 2004
Where: FEI Company, Hillsboro, Oregon
OHSU National Primate Research Center

Thursday Sept 30, 2004
11:30am - Arrival to FEI, registration
12:00 - Lunch
12:30 - Welcome note
- 2 keynote speakers provided by FEI
Speaker I - DualBeam Applications in Biological and Materials Science
Speaker II - Tomography Applications in Biological and Materials Science
1:45pm - break

2:00 - Demonstrations of DualBeam Focused Ion Beam (FIB) instrument, and
the Tomography capability on a Technai TEM (split into 2 groups)
2:45 - break

3:00 - Split into 2 sessions - Biological and Material Science platforms
will
run in parallel.

Material Science:
3:00 - Jun Jiao, Portland State University
3:20 - Joe Robinson Ascend
3:40 - David Basile, HP
4:00 Break
4:10 - Barbara Miner, Intel
4:30 - Eric Sanchez, Portland State U
4:50 - Jeff McDowell, Rontec

Biological Sciences:
3:00 - Elaine Humphrey, University of British Columbia - "Microwave
Processing In a Modern Microscopy Facility"
3:30 - Glen MacDonald, University of Washington Seattle, Virginia
Merrill Bloedel Hearing Research Center
4:00 - mini workshop: "Coloring Electron Micrographs With PhotoShop"
presented by the image processing guru Jim Young

5:15 -6:00 - Poster session
We are soliciting poster presentations in both areas, and strongly
encouraging students to participate in the poster session.
6:00 - Dinner social at the Cornelius Pass Road House and Brewery
(McMenamins).
Poster winners announcement.

Friday Oct. 1, 2004
9:00 Arrival at FEI
9:15 - FEI factory tour (!!!), talk: Emitters and Optics by FEI Beam
Technology.
10:15am Depart from FEI, commute (only minutes drive) to the OSHU's
Primate Research Center.
10:30 - 11am Tour of the Primate Center.
11am -2pm Biological session at the conference room, tour of Imaging
and Morphology Core Facility (Anda Cornea lab director). Lunch will be
arranged in the cafeteria within the PC.
2pm - departure.

Alice Dohnalkova
PNMS President
Environmental Microbiology
Pacific Northwest National Laboratory
Richland, WA 99352
(509) 372-0692 office
(509) 376-3654 TEM lab

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 22 12:20:41 2004

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} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I work for JEOL in the UK. We use them fairly frequently and they
generally work pretty well.

Normally, the standard systems only cancel AC fields - DC
cancellation is usually an option, although it is very rare that DC
fields need to be cancelled - the SEM alignment does that for you
unless the DC field is very high.

You need to look at the wave form of the AC fields - a coil and an
oscilloscope is all you need. If the wave form is fairly stable and
changes only relatively slowly, then cancellation will work well. If
it is changing a lot, then you may have problems - the control box on
the system we use has a LED to indicate when the field is cancelled
or if it is still calculating the wave form. In some cases, it will
help if you have two sensor boxes - these need to be placed as near
to the sample as possible, usually on either side of the chamber.
--
Larry Stoter
PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 22 12:20:23 2004

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} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

If you want top quality photo-standard prints, you really need a
dye-sublimation printer. Quite a bit more expensive to purchase and
to run than a laser printer but the results are much better. Codonics
make several printers aimed specifically at the medical imaging
market.
--
Larry Stoter
PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 22 13:15:45 2004

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} When we use our TEM at low magnification (below 4,000X), we
} sometimes get a bright spot in the middle of pictures we take. I
} always thought the microscope setting was the only thing responsible
} for this problem. But when I checked into a latest episode, I found
} pictures from one particular specimen (thin sections on Formvar
} coated grid) had it, but others from a different specimen (negative
} staining on Farmvar/carbon coated grid) did not, even though all
} pictures were taken with the same scope setting. Has anyone else
} experienced the same? Can anyone explain to me thoroughly how this
} problem occurs, and how to avoid it. Thank you very much in advance.
}
} Hong
} Emory EM

The most likely cause is removal of the plastic due to the beam --
resulting in an area of lowered density. This is quite common (and
can sometimes be used to enhance the contrast of sectioned
specimens). What is probably happening is that someone is
investigating the specimen at high magnification with the beam
condensed to a small spot. When you then go to a lower magnification,
you will see the area where the beam spot has etched away the
plastic. Also, some people focus the image with the condenser reduced
in size (since it much brighter). If too much time is spent in this
condition, you will see the etching of the plastic after the beam is
spread.

One work-around would be to record the images at low mag FIRST and
then do the high mag work (where the condenser is reduced in size)
AND avoid focusing at crossover.

We sometimes use this with specimens of low contrast (LR White and
Spurr's, for example). As the plastic is removed by the beam, the
remaining specimen appears with greater contrast. In fact, our TEM
even has this capability programmed in by the manufacturer (the beam
is traversed over the specimen to "stabilize" the plastic--and it
also improves contrast).
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 22 13:17:35 2004

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} When we use our TEM at low magnification (below 4,000X), we
} sometimes get a bright spot in the middle of pictures we take. I
} always thought the microscope setting was the only thing responsible
} for this problem. But when I checked into a latest episode, I found
} pictures from one particular specimen (thin sections on Formvar
} coated grid) had it, but others from a different specimen (negative
} staining on Farmvar/carbon coated grid) did not, even though all
} pictures were taken with the same scope setting. Has anyone else
} experienced the same? Can anyone explain to me thoroughly how this
} problem occurs, and how to avoid it. Thank you very much in advance.
}
} Hong
} Emory EM

The most likely cause is removal of the plastic due to the beam --
resulting in an area of lowered density. This is quite common (and
can sometimes be used to enhance the contrast of sectioned
specimens). What is probably happening is that someone is
investigating the specimen at high magnification with the beam
condensed to a small spot. When you then go to a lower magnification,
you will see the area where the beam spot has etched away the
plastic. Also, some people focus the image with the condenser reduced
in size (since it much brighter). If too much time is spent in this
condition, you will see the etching of the plastic after the beam is
spread.

One work-around would be to record the images at low mag FIRST and
then do the high mag work (where the condenser is reduced in size)
AND avoid focusing at crossover.

We sometimes use this with specimens of low contrast (LR White and
Spurr's, for example). As the plastic is removed by the beam, the
remaining specimen appears with greater contrast. In fact, our TEM
even has this capability programmed in by the manufacturer (the beam
is traversed over the specimen to "stabilize" the plastic--and it
also improves contrast).
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 05:45:26 2004

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FYI, here is a current job opening at the GE Global Research Center in
Niskayuna, NY. To apply, please go to www.gecareers.com
{http://www.gecareers.com} and enter job #372689.

Materials Scientist/ Microscopist

Business Unit:

GE Global Research

Function:

Engineering/Technology

Location:

NISKAYUNA, NY

Job #:

372689
Posted: Aug 12, 2004

Job Description:
Responsibilities

The Microstructural and Surface Sciences Laboratory is involved in research
into the structure and composition of materials in support of development
programs both at GEGR and at GE businesses. Staff members are expected to
work independently with a high level of expertise, and to become involved
with a number of major project teams. The successful candidate will execute
research projects using advanced electron beam analysis of bulk materials
(e.g., HRSEM, EDS, EBSD, FIB) to study the structure and composition of
materials, including metals, ceramics, composites, polymers, and electronic
materials. Lead in the development of new advanced techniques for materials
characterization. Participate in or lead project teams with the goal of
developing new processes and materials.

Qualifications

BS required, MS preferred in Materials Science, Chemistry, or Physics.
Extensive training and experience in cutting-edge materials characterization
techniques, particularly electron beam techniques. Demonstrated technical
leadership capabilities with excellent teaming and communication skills.
THIS POSITION REQUIRES UNRESTRICTED U.S. WORK AUTHORIZATION (US citizen or
permanent resident status required).

General
We offer a competitive salary, outstanding benefits package and the
professional advantages of an environment that supports your development and
recognizes your achievements. We are an Equal Opportunity Employer.

****************************************************************************
**************************************
Katharine Dovidenko, Ph.D.
Materials Scientist
Microstructural and Surface Sciences Laboratory
GE Global Research Center
K-1-2C12, 1 Research Circle
Niskayuna, NY 12309
Phone: 518-387-4759
Fax: 518-387-6972
Email: dovidenk-at-research.ge.com {mailto:dovidenk-at-research.ge.com}

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 08:34:28 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rdesilva-at-ipn.mx) from on Wednesday, September 22, 2004 at 08:59:35
---------------------------------------------------------------------------

Email: rdesilva-at-ipn.mx
Name: Claudia Douriet

Organization: CIIDIR

Education: Graduate College

Location: Guasave, Sinaloa, M�xico

Question: Hi!
I just started to use a Leica stereomicroscope for my Bachelor degree work and unfortunately I never used one before. I am taking pictures of zooplanktonic organisms and I dont know how much magnification does a picture has. I am not sure if you have to multiply the zomm, by the objective and by the ocular numbers. Can you help me? Thank you very much.

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From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 08:55:24 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (zerfasp-at-ors.od.nih.gov) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 22, 2004 at 15:24:37
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Email: zerfasp-at-ors.od.nih.gov
Name: Patricia Zerfas

Organization: NIH

Title-Subject: [Microscopy] [Filtered] MListserver: Bone Fixation

Question: Does anyone have a protocol for fixing mouse bone tissue? The investigator does not have a microwave nor does she plan to profuse the animals.
Thanks.

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From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 08:55:37 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (john.dumont-at-ctel.net) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 22, 2004 at 16:26:34
---------------------------------------------------------------------------

Email: john.dumont-at-ctel.net
Name: John dumont

Organization: Continental Consulting

Title-Subject: [Microscopy] [Filtered] Olympus EHT Scope

Question: I'm presently refurbishing an older Olympus Model EHT with a DO head. I require a lower and upper lamp assemblies, as well as an owner/operator's manual.

Any suggestions would be appreciated.

Thanks

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 11:06:25 2004

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Thank you for your input. I definitely have seen this removal of the
plastic phenomenon on scope. But I am not convinced that is all what
was happening here. The spot was there even if we moved away from
etched area. Also, the spot sometimes was so intense that it could be
seen on phosphor screen. Someone suggested that it has something to do
with the distance between the pole piece and the objective aperture. I
think I agree, but it seemed that the type of sample prep played a role
in it too.

Thank you all.

Hong

On Sep 22, 2004, at 2:14 PM, John J. Bozzola wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} } When we use our TEM at low magnification (below 4,000X), we
} } sometimes get a bright spot in the middle of pictures we take. I
} } always thought the microscope setting was the only thing responsible
} } for this problem. But when I checked into a latest episode, I found
} } pictures from one particular specimen (thin sections on Formvar
} } coated grid) had it, but others from a different specimen (negative
} } staining on Farmvar/carbon coated grid) did not, even though all
} } pictures were taken with the same scope setting. Has anyone else
} } experienced the same? Can anyone explain to me thoroughly how this
} } problem occurs, and how to avoid it. Thank you very much in advance.
} } Hong
} } Emory EM
}
} The most likely cause is removal of the plastic due to the beam --
} resulting in an area of lowered density. This is quite common (and can
} sometimes be used to enhance the contrast of sectioned specimens).
} What is probably happening is that someone is investigating the
} specimen at high magnification with the beam condensed to a small
} spot. When you then go to a lower magnification, you will see the area
} where the beam spot has etched away the plastic. Also, some people
} focus the image with the condenser reduced in size (since it much
} brighter). If too much time is spent in this condition, you will see
} the etching of the plastic after the beam is spread.
}
} One work-around would be to record the images at low mag FIRST and
} then do the high mag work (where the condenser is reduced in size) AND
} avoid focusing at crossover.
}
} We sometimes use this with specimens of low contrast (LR White and
} Spurr's, for example). As the plastic is removed by the beam, the
} remaining specimen appears with greater contrast. In fact, our TEM
} even has this capability programmed in by the manufacturer (the beam
} is traversed over the specimen to "stabilize" the plastic--and it also
} improves contrast).
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}
}
======================
Hong Yi
Emory School of Medicine Microscopy Core
Emory University
6215 Woodruff Memorial Research Building
101 Woodruff Circle
Atlanta, GA 30322

Tel: (404) 727-8692 (Office), (404) 712-8491 (Lab)
Fax: (404) 727-3157
Email: hyi-at-emory.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 11:47:17 2004

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} } When we use our TEM at low magnification (below 4,000X), we
} } sometimes get a bright spot in the middle of pictures we take. I
} } always thought the microscope setting was the only thing
} } responsible for this problem. But when I checked into a latest
} } episode, I found pictures from one particular specimen (thin
} } sections on Formvar coated grid) had it, but others from a
} } different specimen (negative staining on Farmvar/carbon coated
} } grid) did not, even though all pictures were taken with the same
} } scope setting. Has anyone else experienced the same? Can anyone
} } explain to me thoroughly how this problem occurs, and how to avoid
} } it. Thank you very much in advance.
} } Hong
} } Emory EM
} =============
} The most likely cause is removal of the plastic due to the beam --
} resulting in an area of lowered density. This is quite common (and
} can sometimes be used to enhance the contrast of sectioned
} specimens). What is probably happening is that someone is
} investigating the specimen at high magnification with the beam
} condensed to a small spot. When you then go to a lower
} magnification, you will see the area where the beam spot has etched
} away the plastic. Also, some people focus the image with the
} condenser reduced in size (since it much brighter). If too much time
} is spent in this condition, you will see the etching of the plastic
} after the beam is spread.
}
} One work-around would be to record the images at low mag FIRST and
} then do the high mag work (where the condenser is reduced in size)
} AND avoid focusing at crossover.
}
} We sometimes use this with specimens of low contrast (LR White and
} Spurr's, for example). As the plastic is removed by the beam, the
} remaining specimen appears with greater contrast. In fact, our TEM
} even has this capability programmed in by the manufacturer (the beam
} is traversed over the specimen to "stabilize" the plastic--and it
} also improves contrast).
}
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} ########################
Hong,
I knew what you were referring to on the low power magnification and
just checked it out on my old Philips 200.

The spot is evident on the screen when in low mag. and hence would be
on the negative if I took a picture. It even shows up on the grid
bar when I scan ONLY when the image is out of focus!

If you have a WOBBLER on your scope use it to get into focus and see
if the light spot disappears. If you do not have a wobbler, put in a
holey grid or of course a thicker section that has a hole or two and
focus on the hole to the point where it is in focus and look for the
spot.
I bet it is no longer there.

Pat Connelly
The University of Pennsylvania
Department of Biology
Philadelphia, PA 19104-6018
215-898-7145
psconnel-at-sas.upenn.edu
***********************

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 13:26:26 2004

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Greetings all,
I'm helping with a project which requires dehydration/clearing of the
specimen for observation using laser scanning using both reflected light
and fluorescence detection. Also, we need to minimize mounting agent
shrinkage in order to preserve the z axis aspect ratio of the specimen.
I've suggested using an ethanol dehydration followed by clearing and
mounting in clove oil, but I'm not sure what problems we might encounter
from auto-fluorescence of the clove oil. Oil of wintergreen would work
also, I've just read that clove oil is a little more tolerant of
incomplete dehydration (and seeing as I'm not performing the dehydration
I figured a little margin for error would be a good idea). Any input on
the relative merits of clove oil/oil of wintergreen or even immersion
oil as a penetrating mounting agent would be appreciated. Thanks.
-Karl

--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 14:42:10 2004

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We use clove oil routinely to clear spider genitalia that is preserved in
ethanol 75%, without any previous dehydration. It works very well. Larger
pieces take longer, but I guess you will be working with very small
preparations.

Other labs use lactic acid or methyl salicylate, but I prefer clove oil.

Mart�n J. Ram�rez
Divisi�n Aracnolog�a
Museo Argentino de Ciencias Naturales
Av. Angel Gallardo 470
C1405DJR Buenos Aires
Argentina
tel +54 11 4982-8370 int. 168
fax +54 11 4982-4494

At 03:24 PM 9/23/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 21:47:43 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Tammy-at-kbwiz.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, September 23, 2004 at 16:19:04
---------------------------------------------------------------------------

Email: Tammy-at-kbwiz.com
Name: Tammy Schwalb

Organization: Schwalb Research

Education: Graduate College

Location: Irvine, CA

Question: I am lookig for an embedding media for a delicate tissue membrane encasing a small sensor surface. The device material is epo fix. I would prefer a hydrophilic material that is easy to cut. Any advice?

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From MicroscopyL-request-at-ns.microscopy.com Thu Sep 23 21:48:24 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (spradhan-at-siu.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, September 23, 2004 at 12:30:09
---------------------------------------------------------------------------

Email: spradhan-at-siu.edu
Name: sailesh pradhan

Organization: southern illinois university, carbondale

Education: Graduate College

Location: carbondale,illinois,USA

Question: i have been thinking about building an experimental setup to test the mechanical properties of thin films under loading using speckle pattern interferometry with electron microscopy...i would like to observe the speckle patters using the electron microscopy facility we have here(S-2460N hitachi)...it seems however that i might have problems placing the loading unit inside the chamber primarily owing to the contract they have here regarding the maintainence of the microscope with hitachi and also due to the fact that there arent supposed to be any EM fields or unapproved active devices inside the chamber...in this regard, i would be glad if you could help me out by providing ways to circumvent the problems involved....have people done that kind of work before? if so then how....how can u replace the motor, for example, to load the specimen?
i shall look forward to your reply...

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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 07:54:31 2004

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Dieter:

We are currently using the Slidebook by Inteligent Imaging Inc. (3I) with
an Olympus IX-81.
[http://www.intelligent-imaging.com/slidebook/overview.php ]

It integrates and operates the IX-81 fully, comes with all its available camera
drivers, and does little other than deconvolution. 3I seems to be readily
working with the camera vendors and Olympus to continually update the
drivers and software. I think it maybe a little less costly than the others but
came as a larger package deal. I've not tried the others - Slide book seems to
work well - makes use of multiple processors and available memory (4GB
RAM) to run pretty fast.

Deconvolution: We're just basic users in deconvolution but it does seem
to me that there has been plenty of published justification that the transition
from confocal to deconvolution is at 15-micrometers, i.e. below 15
deconvolution is better, thicker than 15 confocal is better. For 100um samples
you maybe far better served by confocal.

}
} Email: dieter-at-genetik.uni-bielefeld.de
} Name: Dieter Kapp
}
} Organization: Academic/University
}
} Title-Subject: [Microscopy] [Filtered] MListserver: Which Deconvolution
} Software?
}
} Question: Hello Listers!
}
} We are currently searching for a deconvolution software.
} Mainly we look at GFP-fusions in single cells, monolayers (Sf9) or cell
} aggregates (tobacco BY2), up to 100 �m on a Olympus IX81.
}
} What are your experiences with such a software tool?
} How are the results compared to confocal images of the same specimen (...okay,
} deconvolution can be applied to confocal images too)? Is it worth the money?
}
} The two major competitors in the market are SVI/Huygens and AutoQuant. Which
} product did you choose and why? Is there a side by side test of both softwares
} available? Do they differ in image quality? We only need deconvolution, since
} our imaging software is doing well. Do you have other
} recommendations/suggestions?
}
} Any comment on this issue will be warmly welcomed!
} Thank you in advance,
}
} Dieter
}
} ------------------------------------------------------------
} Dr. Dieter Kapp
} Lehrstuhl fuer Genetik
} Postfach 100131
} Universitaet Bielefeld PHONE: +49 (0)521 106 5620
} D-33501 Bielefeld FAX: +49 (0)521 106 5626
} GERMANY Email: dieter-at-genetik.uni-bielefeld.de
}
} ------------------------------------------------------------
}
}
}
} ---------------------------------------------------------------------------
}
}

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 08:10:01 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (John H. Cross-at-lmco.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 23, 2004 at 13:31:31
---------------------------------------------------------------------------

Email: John H. Cross-at-lmco.com
Name: John H. Cross, CIH

Organization: Lockheed Martin Space Operations

Title-Subject: [Microscopy] [Filtered] MListserver: Occupational Health Aspects of Microscope Usage

Question: We have a group of technicians using microscopes to solder electronic components 10-12 hours per day 6-7 days a week.

Does any professional, academic, or industry organization publish occupational health guidelines for the use of microscopes? A sample of questions I need to address are: How often must an individual take a break from looking through a microscope. Is exposure to light coming through the eyepieces a problem? Has anyone described ergonomically-correct work stations for microscopists?

All comments will be welcome. I particularly need objective standards that I can present to management to justify work practice adjustments.

Thanks,

John Cross

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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 09:04:29 2004

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Todd

what sort of microtome are you using? If it's an LKB/Reichert ultramicrotome you could be aligning specimen and knife when the knife is retracted - this is part of the normal cutting cycle to prevent the block snagging the knife when it returns to the top of its travel. When you complete the first cutting cycle the microtome knife stage jumps forward at least 10s of microns. So when setting up for semi-thin sectioning make sure that this isn't happening - it's quite easy to do when you know how.

I haven't gone into detail in case this is not your problem, but it should be easy enough to check. Let me know if you need more info.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
} From: todd hamm {ripbnowell-at-hotmail.com}

Dear Claudia,

The best way to define magnification is to set-up the microscope to a prescribed magnification and zoom (one which is reproducible) then to take a picture of either a ruler with mm or a stage micrometer. If you are viewing the image on a screen, you can lay an overhead transparency on the screen and draw the length of some convenient "marker bar" (ex: 50 micrometers). You will then always have the magnification available. Also, if you are going to capture digital images, most software gives you the ability to calibrate the magnification.

Because of the nature of both the focusing and zoom mechanism on the stereo microscope, it is more difficult to establish exact magnification, so you may need to repeat this calibration for each test run.

Good hunting!
Barbara Foster
Microscopy/Microscopy Education

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018

^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

At 08:33 AM 9/23/2004, rdesilva-at-ipn.mx wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 12:22:53 2004

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Dear Claudia,

The best way to define magnification is to set-up the microscope to a prescribed magnification and zoom (one which is reproducible) then to take a picture of either a ruler with mm or a stage micrometer. If you are viewing the image on a screen, you can lay an overhead transparency on the screen and draw the length of some convenient "marker bar" (ex: 50 micrometers). You will then always have the magnification available. Also, if you are going to capture digital images, most software gives you the ability to calibrate the magnification.

Because of the nature of both the focusing and zoom mechanism on the stereo microscope, it is more difficult to establish exact magnification, so you may need to repeat this calibration for each test run.

Good hunting!
Barbara Foster
Microscopy/Microscopy Education

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018

^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

At 08:33 AM 9/23/2004, rdesilva-at-ipn.mx wrote:

} ------------------------------------------------------------------------------
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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 12:29:05 2004

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When doing semi-thin sectioning I face off my block to cut, when the
specimen comes across the knife it takes an obscenely thick section
and ruins my block face. I have had problems before where the first
section is a little too thick, but simply backing the knife off a
little bit helped. No matter what I do or change the section is still
cutting too thick, are there any suggestions before I go crazy!
______________________________________

Todd,
Could you give a little more info?

What microtome are you using? The "newer" ones (less than 20 years
old) have back lighting that makes approaching the block so much
easier.

I know that this may be a silly question, but are you sure that
everything is tight? (knife stage, knife holder, specimen chuck,
specimen arm)

How are you approaching the block face? I normally creep up on it
slowly using the knife advance while watching through the binoculars
until I'm shaving off little bits. Then I set up my section thickness
and cut a few thicks manually (rather than with the motor drive).

When you bring your knife up to the block are you sure that the
specimen arm is in the cutting stroke and not in the retracted phase
of the cycle?

Have you had your microtome checked to be sure that the calibration
for the thick sectioning mode is OK?

That pretty much covers all of the mistakes I've made over the years...
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 12:30:50 2004

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Dear Salish,

Again, this might be better done as a scanning probe experiment rather than an SEM experiment. You can readily measure force, adhesion, and viscoelasticity directly.

Contact me offline for details.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018

^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

At 09:47 PM 9/23/2004, spradhan-at-siu.edu wrote:

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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 13:56:58 2004

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_________________________________________________________________
Get ready for school! Find articles, homework help and more in the Back to
School Guide! http://special.msn.com/network/04backtoschool.armx

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 14:07:15 2004

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Sorry I was a little vague before, the ultramicrotome is a fairly new LKB
model. I found that when I take the semi-thins manually the problem isn't
as bad. Is it possible that the belt that controls the specimen advancement
has been worn, I thought that might be possible since the thickness varies
considerably from section to section. But like I said when section manually
it is much less of a problem.

Todd Hamm

_________________________________________________________________
Don�t just search. Find. Check out the new MSN Search!
http://search.msn.click-url.com/go/onm00200636ave/direct/01/

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 14:39:01 2004

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Hello all,

We're looking at mouse embryos in the early
stages using quadrature tomographic microscopy.
What I want to do is rotate an embryo in specific
increments and image at these increments at
various time points (total � 48 hrs). This will
be done in a Bioptechs chamber (no affiliation)
with additional atmospheric control.

My first thought is to "attach" the embryo to a
"tube" via a syringe, with slight negative
pressure. Then, attach a goniometer somehow to
the tube or the syringe.

These lists have been extremely useful in the
past and I thank you all in advance for your
input.

Best,

Gary
--

Gary Laevsky, Ph.D.
Keck Facility Manager, CenSSIS
Northeastern University
302 Stearns
360 Huntington Ave.
Boston, MA 02115

voice (617) 373-8570
fax (617) 373-7783

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 17:51:40 2004

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Hi:

Can anyone fill me in on the details of the EMSA file format for EDS spectra.

Mostly I need to know how to help someone strip out the header so they can
look at it in Excel.

The file I see when I look at it has the header info, followed by what
looks like 4 columns of numbers. I was expecting 2 columns, x and y. What's
up?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 22:49:11 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (timandcathy-at-mho.com ) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Micro-Form.html on Friday, September 24, 2004 at 13:04:20
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Email: timandcathy-at-mho.com
Name: Timothy Personett

School: NA

State: CO

Question: Hi I am looking for a microscope -at- 1600x to look at bacteria, I have seen oil immersion does the microscope need a oil lense or can you just use the oil on the slides ? and any ideas on good brands for the max power ?

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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 23:14:00 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (necipunlu-at-yahoo.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 24, 2004 at 08:51:18
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Email: necipunlu-at-yahoo.com
Name: necip unlu

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hey guys,

I need JEOL2000EX alignment procedure if you have that kind of knowledge, i will be grateful to you.

Thanks alot.

Necip.

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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 23:14:34 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rangari0-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 24, 2004 at 22:21:53
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Email: rangari0-at-yahoo.com
Name: vijay

Organization: tuskegee university

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi
Members
pl let me know where I can buying the glass knifes for microtoming.
Thanks

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From MicroscopyL-request-at-ns.microscopy.com Fri Sep 24 23:23:50 2004

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Jon

The header is a text field which describes how the data is formated.
You should be able to read the entire spectrum in any text editor.
XY columns is only one of many possible formats which are legal
forms for the data set to be output.

The program that is exporting the data into the MSA-MAS file format
should ask the individual how many columns and what format to export
the file into. It sounds like the person requested 4 column format. I'd suggest
that you read the header closely it should explain how the data is
output. You can then edit it as appropriate.

Alternatively have the data re-exported into XY format.

The details of the format can be found here:

http://www.amc.anl.gov/ANLSoftwareLibrary/02-EMMPDL/Xeds/EMMFF/

Nestor

Your Friendly Neighborhood SysOp

Title :EMSAMASFF
Keywords :XEDS,EELS,AES,WDS,CLS,GAM,XRF,PES
Computer :IBM, MAC, DEC
Operating System :ALL
Programming Language :Fortran 77
Hardware Requirements :None
Author(s) :EMSA/MAS TASK FORCE
Ray Egerton ,Charles E. Fiori ,John A. Hunt ,Mike S. Isaacson, Earl J. Kirkland ,Nestor J. Zaluzec
Correspondence Address : R.F. EGERTON CHAIRMAN
University of Alberta
Dept. of Physics
Alberta, Canada,
Abstract:

A simple format for the exchange of digital spectral data is
presented, and proposed as an EMSA/MAS standard. This format is readable by both humans and computers and is suitable for transmission through various electronic networks (BITNET, ARPANET), the phone system (with modems) or on physical computer storage
devices (such as floppy disks). The format is not tied to any one computer, programming language or computer operating system. The adoption of a standard format would enable different laboratories to freely exchange spectral data, and would help to standarize data
analysis software. If equipment manufacturers were to support a common format, the microscopy and microanalysis community would avoid duplicated effort in writing data-analysis software. This version of EMSAMASFF contains two subroutines which read and write spectral data files Version 1.0 data format. The data are stored as simple ASCII characters at a user defined number of columns per line for the length of the data file. The spectral data is preceeded by a series of header lines, which tell the user about the parameters of the spectrum. The header lines are identified by the first character in the line being the symbol (#) followed by a descriptor and if appropriate its units. An example of a data file format can be found in the EMSAMASFF.DOC file.

}
} Hi:
}
} Can anyone fill me in on the details of the EMSA file format for EDS spectra.
}
} Mostly I need to know how to help someone strip out the header so they can
} look at it in Excel.
}
} The file I see when I look at it has the header info, followed by what
} looks like 4 columns of numbers. I was expecting 2 columns, x and y. What's
} up?
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu

From MicroscopyL-request-at-ns.microscopy.com Sun Sep 26 10:59:11 2004

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Hi, Tim

There is a new technology coming from a company called AETOS which is probably just what you need. Contact Sam Lawrence at 334-749-0134. The technology is called "CytoViva". I had a chance to run it last week and was imaging particles at the 100nm level (I think a combo of both detection and resolution).

Hope this is helpful.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018

^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

At 10:48 PM 9/24/2004, timandcathy-at-mho.com wrote:

} ------------------------------------------------------------------------------
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From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 06:42:23 2004

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Hi Todd,
I had a similar problems while cutting semi-thin sections. What I've
noticed it always happend after rough triming. I talked to Leica
representative and he expalained to me that the problem was how
I've been trimming blocks. I advanced knife towards the block and
always at the same time I moved the block in the specimen arm up
and down, but I was not making a full rotation with the wheel. He told
me that even though I do not rotate the specimen arm and it is not
advancing it builds up the distance, meaning that every time I
moved block up and down it advanced the specimen setting without
advancing it physically.( I am not sure if I explained well.) Then when
I switch to an automatic mode all this "build up" adds up and
microtome jumps the specimen forward cutting a big chunk. I
changed my method of trimming and I do not have any problems
anymore.
I hope my explanation will be of help to you.
Dorota

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 07:51:46 2004

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Greetings,
We are submitting a purchase requisition for an image capture system for
our SEM that has an older Microspec WDS spectrometer (pre-Oxford).
Due to budget requirements we are unable to upgrade the WDS system and
the need to capture the data stream from the spectrometer and bring it
into our image capture database is great.
Are there any suggestions to accomplish this capture of the data stream
from the spectrometer and bring it into the computer for further
processing?

Thanks for any help.
Robb

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 08:55:45 2004

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Hi all,

I just ran across this reference in our local newspaper about the care and
storage of CDs and DVDs. It is copublished by CLIR (Council on Library and
Information Resources) and NIST, so it should be reliable info.

http://www.clir.org/pubs/reports/pub121/contents.html

From my quick run through of the article, it appears that DVDs may be
inherently more stable than CDs.

Cheers,
Henk

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all at
once. Lately it doesn't seem to be working.

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 10:34:19 2004

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Hi all,
Despite pending budget cuts, I've been asked to submit an equipment
wish list. Go figure;-)
So wishfully thinking, I'd really like to hear your thoughts on the
various microwave ovens that are available from the EM supply
companies. Any great machines out there? Giving great results!
thanks in advance,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 10:43:24 2004

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Hello Rob,

I am in a similar situation...
SEM: Hitachi S-3500H
EDS: IXRF Systems (Full)
WDS: Microspec 2A w/ Kevex Sesame24 Controller

My WDS controller has an analog ratemeter output for line scans and a
"digital" output for mapping. These signals are input to the "x-ray_1 and
x-ray_2 external inputs on the SEM. Maps and line scans are captured and
stored directly using Hitachi/Quartz software.
FWIW:
Image brightness/contrast manipulation may be required if your SEM works
like my Hitachi. In the mapping mode, I must use slow recursive imaging to
have sufficient integration time (other modes too fast) for most work. The
3500 software does not handle this gracefully. The finished map is usually
totally black because each "dot" luminosity is divided (I infer) by the
number of (recursive) passes and the data summed for presentation. Since
dots may not occur at the same exact location for each pass, they are too
dim to see. Thus, contrast/brightness expansion, using Photoshop, IrFan
View, or the like, is required to see the result. In the linescan mode
dynamic range is about half of what would be expected. The software limits
graphical amplitude excursions of half (or less) of the vertical range of
the crt image.

I often run "spectrum - scans" also, generating data (spectrum graphic)
within a limited wavelength range. My Sesame system has a serial data port
which will allow me to upload the wavelength positions and x-ray intensities
to my EDS PC. Any serial terminal program should work. The saved data can
be sent to a spreadsheet, or wherever.

If you have a 3PC? instead of the Kevex Sesame, I cannot be so specific...

Hope this is helpful.

Woody

-----Original Message-----
} From: Robb Westby [mailto:robbw-at-mxim.com]
Sent: Monday, September 27, 2004 8:51 AM
To: microscopy-at-msa.microscopy.com

Greetings,
We are submitting a purchase requisition for an image capture system for
our SEM that has an older Microspec WDS spectrometer (pre-Oxford).
Due to budget requirements we are unable to upgrade the WDS system and
the need to capture the data stream from the spectrometer and bring it
into our image capture database is great.
Are there any suggestions to accomplish this capture of the data stream
from the spectrometer and bring it into the computer for further
processing?

Thanks for any help.
Robb

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 11:06:06 2004

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Hi,

I think there was an article in some magazine months ago on how
reliable CD-R are for archival. Depending on the brand (or no-brand if you
prefer) it can fail after less than 2 years. That was much less than the
20-100 years promised by the maker. People were recomending to use CD-RW
for archival purpose instead. Since it doesn't use a dye for recording the
data should be less affected by the problem. I suppose the same applies
for DVD+/-R and DVD+/-RW. Regards,

Carlos

On Mon, 27 Sep 2004, Hendrik O. Colijn wrote:

}
} Hi all,
}
} I just ran across this reference in our local newspaper about the care and
} storage of CDs and DVDs. It is copublished by CLIR (Council on Library and
} Information Resources) and NIST, so it should be reliable info.
}
} http://www.clir.org/pubs/reports/pub121/contents.html
}
} From my quick run through of the article, it appears that DVDs may be
} inherently more stable than CDs.
}
} Cheers,
} Henk
}
}

o-------------------------------------------------------o
| Carlos Kazuo Inoki |
| Department of Physics - University at Albany |
| 1400 Washington Ave.- Albany - NY - 12222 |
o-------------------------------------------------------o

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 12:26:47 2004

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Henk,

That's an extremely informative and useful article. Thank you!

It was interesting to learn that CD RW and DVD RW (bits encoded in metal structure
by a phase change from crystalline to amorphous) are less stable over time than CD
R and DVD R (bits encoded in degradable organic dyes). And that RW discs that are
"cycled" a lot (many re-writes) will degrade faster.

The best advice seems to be to use R (write once) discs (CD or DVD) and store them
vertically in a cool, low-light environment. Of course, transferring to fresh
media every year or so couldn't hurt...

Mike

"Hendrik O. Colijn" wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi all,
}
} I just ran across this reference in our local newspaper about the care and
} storage of CDs and DVDs. It is copublished by CLIR (Council on Library and
} Information Resources) and NIST, so it should be reliable info.
}
} http://www.clir.org/pubs/reports/pub121/contents.html
}
} From my quick run through of the article, it appears that DVDs may be
} inherently more stable than CDs.
}
} Cheers,
} Henk
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://www.ceof.ohio-state.edu
} Time is that quality of nature which keeps events from happening all at
} once. Lately it doesn't seem to be working.

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 12:54:34 2004

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} Name: John H. Cross, CIH
} Organization: Lockheed Martin Space Operations
} Title-Subject: [Microscopy] [Filtered] MListserver: Occupational
} Health Aspects of
} Microscope Usage
} Question: We have a group of technicians using microscopes to solder
} electronic
} components 10-12 hours per day 6-7 days a week.
}
} Does any professional, academic, or industry organization publish
} occupational health
} guidelines for the use of microscopes? A sample of questions I need
} to address are:
} How often must an individual take a break from looking through a microscope.
} Is exposure to light coming through the eyepieces a problem?
} Has anyone described ergonomically-correct work stations for microscopists?
}
} All comments will be welcome. I particularly need objective
} standards that I can present
} to management to justify work practice adjustments.
}
} John Cross
Email: John H. Cross-at-lmco.com
==============
John,
I am not aware of the reference that you requested. However now that
I am in my mid-50's ,
I recently have gotten several work related physical problems.

When I am using any scope for an extended period of time my eyes fail
to focus properly
for distance. This I first noticed after nearly a whole day on my
TEM, doing a lot of
scanning for a particular cell type in sections. When I left the
building for the night I
could not see properly anything that was more than a few yards in
front of me. I realized
that this had been happening over an extended period of time but it
was not so disturbing as
it had become. I consulted my eye doctor and he said that this was
not abnormal for people
my age. The eye muscles are not as elastic as they were a few years
ago hence they do not
snap back like they used to. I need to stop "scoping" every 15
minutes or so and give my
muscles a workout by focusing on a particular object in the distance
for a short while.
This has been working great for me after I got used to the short
interruptions and my concentration was improved because of the short
distraction from what can be very boring,
when what I am looking for is difficult to find.

A second problem is the nerve that goes through my elbow. Everyone
has heard of carpel
tunnel syndrome. There is a similar area in the elbow. The test for
this is to extend the
arms straight out to the front, at shoulder height with the backs of
the hands facing
upwards.
Make a fist, flex the wrist to raise just the fist upwards. If there
is no pain, it is OK,
but if you are truly having a problem the pain can be quite intense.
I would suggest that
one contact the doctor about this. My case was already very painful
when I went to the
doctor, thinking that it would get better itself - mistake! I
received several shots into the
area over an 8 week period because I can not take oral anti-inflammatory drugs.
I also had to do exercises several times a day to strengthen the
surrounding muscles.
In the mean time I took several older mouse-pads, cut them in half,
stacked them so that
my lower arm/elbow is at least as high as my hand when using the controls.
When using the hands to solder, one may just need more cushioning
under the elbow.
I have seen advertised scopes that claim to be more ergonomical but
at the moment
I can not recall the make.

If this is not already being done, I should expect that the fumes
from the solder should be
sucked away from the scope and go through a suitable filter so that
they are not inhaled
by the tech.

Pat Connelly
The University of Pennsylvania
Department of Biology
Philadelphia, PA 19104-6018
215-898-7145
psconnel-at-sas.upenn.edu

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 13:16:21 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear John,
I am the Ergonomics Representative for our department at UBC and the
guidelines I was given for people working at computer terminals all day was:
a one minute break every ten minutes and a ten-minute break every hour. The
short break was just to ease the eye strain of looking at a fixed focus for
extended periods and just consists of focussing out the window or at a
longer distance and relaxing arms, hands, neck, etc. The longer hour break
was to stand up, stretch, move around and relax. These were just a
guideline. There are also things about the setup to check, such as the angle
of arms, wrists, hips, knees, the height of chair and height of monitor.
Your case is special, but you should be able to work out, with the people
doing the work, a checklist of things to adjust to make the work
ergonomically sound or as least-damaging as possible. Good support chairs
with lots of adjustment, microscopes that can adjust eyepiece spacing,
armrests are just some of the things that might help.
The light coming from the microscope should not be a problem, but should be
controlled by the user so it can be adjusted to a comfortable level. The
breaks are important to relieve eye-strain.
Good luck,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "by way of MicroscopyListserver" {John.H.Cross-at-lmco.com}
To: {microscopy-at-microscopy.com}
Sent: Friday, September 24, 2004 6:08 AM

Pat;

Have you tried contacting OSHA [Occupational Safety and Health
Aministration]? OSHA is a US Gov't agency. http://www.osha.gov

Peter Tomic
Agere Systems
Allentown, PA

-----Original Message-----
} From: Pat Connelly [mailto:psconnel-at-sas.upenn.edu]
Sent: Monday, September 27, 2004 1:54 PM
To: by way of MicroscopyListserver
Cc: microscopy-at-msa.microscopy.com

} Name: John H. Cross, CIH
} Organization: Lockheed Martin Space Operations
} Title-Subject: [Microscopy] [Filtered] MListserver: Occupational
} Health Aspects of
} Microscope Usage
} Question: We have a group of technicians using microscopes to solder
} electronic
} components 10-12 hours per day 6-7 days a week.
}
} Does any professional, academic, or industry organization publish
} occupational health guidelines for the use of microscopes? A sample of

} questions I need to address are:
} How often must an individual take a break from looking through a
microscope.
} Is exposure to light coming through the eyepieces a problem?
} Has anyone described ergonomically-correct work stations for
microscopists?
}
} All comments will be welcome. I particularly need objective standards
} that I can present to management to justify work practice adjustments.
}
} John Cross
Email: John H. Cross-at-lmco.com
==============
John,
I am not aware of the reference that you requested. However now that
I am in my mid-50's ,
I recently have gotten several work related physical problems.

When I am using any scope for an extended period of time my eyes fail
to focus properly
for distance. This I first noticed after nearly a whole day on my
TEM, doing a lot of
scanning for a particular cell type in sections. When I left the
building for the night I
could not see properly anything that was more than a few yards in
front of me. I realized
that this had been happening over an extended period of time but it
was not so disturbing as
it had become. I consulted my eye doctor and he said that this was
not abnormal for people
my age. The eye muscles are not as elastic as they were a few years
ago hence they do not
snap back like they used to. I need to stop "scoping" every 15
minutes or so and give my
muscles a workout by focusing on a particular object in the distance
for a short while.
This has been working great for me after I got used to the short
interruptions and my concentration was improved because of the short
distraction from what can be very boring,
when what I am looking for is difficult to find.

A second problem is the nerve that goes through my elbow. Everyone
has heard of carpel
tunnel syndrome. There is a similar area in the elbow. The test for
this is to extend the
arms straight out to the front, at shoulder height with the backs of
the hands facing
upwards.
Make a fist, flex the wrist to raise just the fist upwards. If there
is no pain, it is OK,
but if you are truly having a problem the pain can be quite intense.
I would suggest that
one contact the doctor about this. My case was already very painful
when I went to the
doctor, thinking that it would get better itself - mistake! I
received several shots into the
area over an 8 week period because I can not take oral anti-inflammatory
drugs. I also had to do exercises several times a day to strengthen the
surrounding muscles.
In the mean time I took several older mouse-pads, cut them in half,
stacked them so that
my lower arm/elbow is at least as high as my hand when using the
controls. When using the hands to solder, one may just need more
cushioning
under the elbow.
I have seen advertised scopes that claim to be more ergonomical but
at the moment
I can not recall the make.

If this is not already being done, I should expect that the fumes
from the solder should be
sucked away from the scope and go through a suitable filter so that
they are not inhaled
by the tech.

Pat Connelly
The University of Pennsylvania
Department of Biology
Philadelphia, PA 19104-6018
215-898-7145
psconnel-at-sas.upenn.edu

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 15:38:57 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

Try these websites. Maybe they will help you.

http://www.office-ergo.com/
http://www.niehs.nih.gov/odhsb/ergoguid/home.htm
http://www.dehs.umn.edu/ergo/lab/
http://www.osha.gov/SLTC/laboratories/index.html

I will also send you a ppt-presentation that I did for a lab 'safety'
meeting.

Kelly A. Ramos
Metallurgical Engineer / Supervisor
Argo-Tech Materials Laboratories
23555 Euclid Avenue
Cleveland, OH 44117
216-692-5904 or 216-692-5446
(fax) 216-692-5816
http://www.atclabs.com

****************************************************************

JOHN WROTE:
------------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America

} Name: John H. Cross, CIH
} Organization: Lockheed Martin Space Operations
} Title-Subject: [Microscopy] [Filtered] MListserver: Occupational
} Health Aspects of
} Microscope Usage
} Question: We have a group of technicians using microscopes to solder
} electronic
} components 10-12 hours per day 6-7 days a week.
}
} Does any professional, academic, or industry organization publish
} occupational health
} guidelines for the use of microscopes? A sample of questions I need
} to address are:
} How often must an individual take a break from looking through a
microscope.
} Is exposure to light coming through the eyepieces a problem?
} Has anyone described ergonomically-correct work stations for
microscopists?
}
} All comments will be welcome. I particularly need objective
} standards that I can present
} to management to justify work practice adjustments.
}
} John Cross
Email: John H. Cross-at-lmco.com

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 19:50:02 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all;

Can someone tell me if such a data base exist? I am hoping to get
information on rates and annual budget of university EM facilities in
the US. Specifically, I am hoping to know the percentage of charge
based income vs. university support. If there is not such a data base,
would you EM facility directors mind emailing me and giving me some
idea about the case in your facility. Thank you all very much in
advance.

Hong
Emory EM.

From MicroscopyL-request-at-ns.microscopy.com Mon Sep 27 20:56:57 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mdawes-at-scu.edu.au) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 27, 2004 at 19:33:49
---------------------------------------------------------------------------

Email: mdawes-at-scu.edu.au
Name: Maxine Dawes

Organization: Southern Cross University

Title-Subject: [Microscopy] [Filtered] Fluorochrome stain - Light Microscope

Question: We have a Reflected light fluorescence attachment CX-RFL which fits onto our Olympus Compound microscope CX 40. The Cube Dichroic Mirror/Filter combinations are: a). CX-DMB (Mirror cube), Exciter filter BP475, Barrier filter 05151F and b). CX-DMG (Mirror cube), Exciter filter BP545, Barrier filter 0590. My question is:
We are trying to stain some pollen tubes to photograph the pollen tube growth - all papers that I have read seem to use the fluorescing stain Analine Blue which stains the cellulose � we do not have a Mirror cube to view this stain and after doing some research with our suppliers we cannot use Aniline blue with the set up we have. We can use the fluorochrome FITC . As there seem to be several stains Fluoresceine diacetate FDA (which appears to stain for protein) and Fluoresceine isothiocyanates FITC (which also appears to stain for protein), my question is can we use either of these stains with our system to photograph pollen tube growth and which one is the easier of the two and is there a tried and tested procedure that someone might be willing to share with us.

Regards
Maxine

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 03:13:37 2004

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I�m worried about my health too.
My question: Is there any harmful radiation working with
JEOL 100-S electron microscope?

K�rt Padari
University of Tartu
kartp-at-ut.ee

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 03:20:12 2004

Contents Retrieved from Microscopy Listserver Archives
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Torino 28 September 2004
(ITALY)

Hi all,

Does anyone have an experience or some information about how to realize a sharp cutting edge by hand working an iron piece?
I�d like to use it for cutting wax in an hand microtome.
I�d like to know the cutting angle, the abrasive powders to use and the operating steps.
Thank you.
Kindly Regards,

Massimo

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 08:19:14 2004

Contents Retrieved from Microscopy Listserver Archives
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Maxine,
It was probably a typo in your post, but analine blue stains
callose (as well as some other polysaccharides), not cellulose. You
probably also know that Biosupplies in Mebourne sells a cleaned up
version of the dye called Sirofluor that is more speciific for
callose, and often gives nicer images, but is still uv excited.

Have you tried imaging analine blue stained stuff with your
blue cube? It just might work--I think that analine blue has a pretty
broad excitation spectrum.

HTH,
Tobias

}
}
} Email: mdawes-at-scu.edu.au
} Name: Maxine Dawes
}
} Organization: Southern Cross University
}
} Title-Subject: [Microscopy] [Filtered] Fluorochrome stain - Light Microscope
}
} Question: We have a Reflected light fluorescence attachment CX-RFL
} which fits onto our Olympus Compound microscope CX 40. The Cube
} Dichroic Mirror/Filter combinations are: a). CX-DMB (Mirror cube),
} Exciter filter BP475, Barrier filter 05151F and b). CX-DMG (Mirror
} cube), Exciter filter BP545, Barrier filter 0590. My question is:
} We are trying to stain some pollen tubes to photograph the pollen
} tube growth - all papers that I have read seem to use the
} fluorescing stain Analine Blue which stains the cellulose � we do
} not have a Mirror cube to view this stain and after doing some
} research with our suppliers we cannot use Aniline blue with the set
} up we have. We can use the fluorochrome FITC . As there seem to be
} several stains Fluoresceine diacetate FDA (which appears to stain
} for protein) and Fluoresceine isothiocyanates FITC (which also
} appears to stain for protein), my question is can we use either of
} these stains with our system to photograph pollen tube growth and
} which one is the easier of the two and is there a tried and tested
} procedure that someone might be willing to share with us.
}
} Regards
} Maxine
}
}
} ---------------------------------------------------------------------------

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 08:20:03 2004

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Your service engineer should monitor radiation leaking
during operation. After 23yrs our SEM has just started to
leak (at 30kV only). I am having a lead shield fitted to
block the leak.

Dave

On Tue, 28 Sep 2004 11:21:35 +0300 (EEST) Kart Padari
{kartp-at-ut.ee} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
}
} I�m worried about my health too.
} My question: Is there any harmful radiation working with
} JEOL 100-S electron microscope?
}
} K�rt Padari
} University of Tartu
} kartp-at-ut.ee
}
}
}
}
}
}
} This incoming email to UWE has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"

This email has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 08:45:09 2004

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Hong,
You can see the rates for my 2 facilities (EM and Optical Microscopy)
on the school's website at:
http://www.med.cornell.edu/research/cores/index.html

We are subsidized by the College to help cover staff salaries, we are
not charged "overhead" costs. Otherwise, the 2 facilities that I'm
involved with cover all other costs.

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 09:10:54 2004

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

K�rt Padari wrote:
=====================================================
I�m worried about my health too.
My question: Is there any harmful radiation working with
JEOL 100-S electron microscope?
=====================================================
My first exposure to an EM was in 1963, the round screen "horizontal"
Philips 100 TEM. I was told (but don't know from my own knowledge that
this was true) that some of these earliest models were manufactured with
ordinary instead of lead glass. I also had contact with an early RCA TEM,
I think it was an EMU-1 (or something close to that). Those are the only two
EMs I have ever heard about that had inherent radiation problems and once
the problem was recognized, retrofits were installed, taking away the
problems. I have met operators of those early RCA instruments who
literally did their work wearing a lead apron. My sense if that there are
none of these old generation EMs in use any more.

Instruments manufactured after that era were designed such that the mass of
the column itself would be sufficiently massive and absorbing of x-rays,
that raditation just could not escape during operation. True, one could
envision that a column could be sufficiently misaligned that x-rays could
possibly get out, but the instruments seem to be designed in a way that if
they really were misaligned by that extent, there would be a vacuum leak and
the high voltage would automatically shut down. Some very many years ago,
we tried this with a JEOL 100CX and could not get the microscope
sufficiently misaligned to allow x-rays to get out. With a JSM-U3 SEM, at
that same time, the column would lose vacuum before it could be misaligned
sufficiently to allow x-rays to come through.

But there are at least several circumstances in which radiation can escape:

a) Someone breaks the lead glass viewing screen of a TEM and thinking they
are saving money, replaces the broken glass with ordinary glass. I have
heard of this very thing happening, on occasion over the years. Such a
replacement of the glass would be picked up instantly during any kind of a
radiation survey, however. Without such a survey, it is not possible to
differentiate, by appearance, lead vs. non-lead glass.

b) Adjustment knobs for the lenses seem to be slightly (x-ray) "leaky" in
some instruments, but the exposure is localized to the area of the knobs,
and therefore also to the fingers. With modern instruments where this is
done by the computer, this risk is much less and might no longer be present.
I have heard of people wrapping lead foil around their several fingers when
adjusting the knobs but I don't know if that is necessarily a "recommended"
procedure. But a general room survey would not necessarily reveal this
exposure risk.

c) Home made or radically altered commercial instruments which do not have
the "safety engineering" built into them that would otherwise be found in a
commerical instrument. I have always thought that this type of instrument
should be surveyed quite frequently because of the frequency that changes
seem to be made to the basic instrument.

Much of the motivation for those who do radiation surveys involving
commercially purchase instruments is to protect the organization from
lawsuits from former or present employees who make the claim that some
medical condition was either caused or aggrivated by their operation of
their TEM or SEM. Considering all the persons who have used both SEMs and
TEMs over the years, if there was some health issue, I would have thought
that it would have shown up clinically by now. When considering all the
risks in an EM loboratory, I have always thought that exposure to the
embedding resin chemicals would represent a far greater health risk than
operating a column instrument.

This is a very important topic and if there are other perspectives on this,
I would surely like to hear them.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 10:15:45 2004

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I would hope that these rates are not for the general public and are
restricted to in-house use. Those of us in the private commercial labs are
not subsidized by our own tax dollars. This is always an issue for us with
faculty taking on outside work and using school resources.

My $0.02 as a local professor was soliciting his services only
yesterday's. My associate was a upset with the lack of awareness and
sensitivity to the unfair tax advantage.

Alan Stone
ASTON

At 08:45 AM 9/28/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 10:43:01 2004

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Dave,
Could you please elaborate? I'm having a little trouble imagining what
could change (other than physical modifications) that would allow leakage.

Is the newest survey perhaps simply more sensitive than previous surveys?

What brand and model?

Where has it started leaking?

What kind of intensity?

I service a lot of older scopes (as much as 10 years older than yours) and
need to know if this should be more closely watched.

Thanks for your time and help.

Ken Converse

owner�
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
} From: Patton, David [mailto:David.Patton-at-uwe.ac.uk]
Sent: Tuesday, September 28, 2004 9:20 AM
To: Kart Padari
Cc: microscopy-at-msa.microscopy.com

Your service engineer should monitor radiation leaking
during operation. After 23yrs our SEM has just started to
leak (at 30kV only). I am having a lead shield fitted to
block the leak.

Dave

On Tue, 28 Sep 2004 11:21:35 +0300 (EEST) Kart Padari
{kartp-at-ut.ee} wrote:

}
}
}
----------------------------------------------------------------------------
--
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
---
}
}
}
} I�m worried about my health too.
} My question: Is there any harmful radiation working with
} JEOL 100-S electron microscope?
}
} K�rt Padari
} University of Tartu
} kartp-at-ut.ee
}
}
}
}
}
}
} This incoming email to UWE has been independently scanned for viruses and
any virus detected has been removed using McAfee anti-virus software

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"

This email has been independently scanned for viruses and any virus detected
has been removed using McAfee anti-virus software

___________________________________________________________
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From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 10:54:28 2004

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Alan, and others
My rates are for grant-funded "in house" personnel...we add a
whopping surcharge to outside users to compensate for the fact that
they don't contribute to the overhead costs of the College as do our
grant-funded people.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 13:00:53 2004

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Leona,

Thank you for clarifying that.

Alan

At 12:37 PM 9/28/2004, you wrote:
} Alan, and others
} My rates are for grant-funded "in house" personnel...we add a whopping
} surcharge to outside users to compensate for the fact that they don't
} contribute to the overhead costs of the College as do our grant-funded people.
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 13:58:37 2004

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On Sep 28, 2004, at 1:12 AM, Kart Padari wrote:

} I�m worried about my health too.
} My question: Is there any harmful radiation working with
} JEOL 100-S electron microscope?
}
Dear K�rt,
There should not be any radiation problems; however, not all EMs have
been properly maintained, so it is best to check. The best ways are to
use a hand-held ion chamber detector to survey the area while the scope
is operated in the most extreme conditions, such as putting a Pt
aperture in the stage and hitting the aperture with the most intense
beam you can produce, moving the shift and tilt controls through the
full extent of their range, etc. Another way is to place film badges
or thermoluminescent detectors around the room and measure the
background on a month-by-month schedule. You should decide where a
radiation leak is most likely by looking at the location of shielding
pieces and thin spots in the column wall, and you need to measure the
areas where a radiation leak, however unlikely, would do the most
damage--obviously where the operator sits, where specimens are loaded,
or anywhere that someone is expected to spend a fair amount of time.
Remember that x-rays can penetrate walls and floors, so check for
radiation that could harm someone in the lab above, below, across the
hall, or other possible locations. Thick concrete is a much better
shielding material than wood or sheet metal, so more care is required,
if your scope is surrounded by the latter. Take the time to make this
check, then you will be able to operate with greater peace of mind.
Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 16:43:57 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (donaldawbrey-at-texashealth.org) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, September 28, 2004 at 09:22:18
---------------------------------------------------------------------------

Email: donaldawbrey-at-texashealth.org
Name: Donald G. Awbrey HT(ASCP) QIHC

Organization: Harris Methodist Hospital

Title-Subject: [Microscopy] [Filtered] TEM Laser Printer

Question: Dear Micro netters,

We are looking for a high resolution printer for our TEM workstation.
I've seen monochrome lazer printers that have a 1200 X 1200 dpi
resolution. Our digital camera will be a 2K camera. Will this
printer print photos of diagnostic quality? Or would we have to go
with a dye sublimation printer? I've noticed that these sub printers
are very expensive, but have a "spatial" resolution of around 320
dpi. Is this the same as regular resolution? If so, then the laser
printer would have better resolution. This does not make sense. Is
1200 X 1200 dpi good enough for CAP inspection.

Any information on diagnostic quality printers for TEM will be
greatfully appreciated.

Thank you in advance.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 17:38:43 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chuck;
Chuck;
When I was in grad school, we purchased a used JSM-U3 through JEOL that had been in previous use at the GM tech center. The stage door was EXTREMELY leaky under normal use conditions (it was different than our other JSM-U3). I eventually installed a lead lining in the door.

John Mardinly
Intel

-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: Tuesday, September 28, 2004 8:12 AM
To: MICROSCOPY BB

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

K�rt Padari wrote:
=====================================================
I�m worried about my health too.
My question: Is there any harmful radiation working with
JEOL 100-S electron microscope?
=====================================================
My first exposure to an EM was in 1963, the round screen "horizontal"
Philips 100 TEM. I was told (but don't know from my own knowledge that
this was true) that some of these earliest models were manufactured with
ordinary instead of lead glass. I also had contact with an early RCA TEM,
I think it was an EMU-1 (or something close to that). Those are the only two
EMs I have ever heard about that had inherent radiation problems and once
the problem was recognized, retrofits were installed, taking away the
problems. I have met operators of those early RCA instruments who
literally did their work wearing a lead apron. My sense if that there are
none of these old generation EMs in use any more.

Instruments manufactured after that era were designed such that the mass of
the column itself would be sufficiently massive and absorbing of x-rays,
that raditation just could not escape during operation. True, one could
envision that a column could be sufficiently misaligned that x-rays could
possibly get out, but the instruments seem to be designed in a way that if
they really were misaligned by that extent, there would be a vacuum leak and
the high voltage would automatically shut down. Some very many years ago,
we tried this with a JEOL 100CX and could not get the microscope
sufficiently misaligned to allow x-rays to get out. With a JSM-U3 SEM, at
that same time, the column would lose vacuum before it could be misaligned
sufficiently to allow x-rays to come through.

But there are at least several circumstances in which radiation can escape:

a) Someone breaks the lead glass viewing screen of a TEM and thinking they
are saving money, replaces the broken glass with ordinary glass. I have
heard of this very thing happening, on occasion over the years. Such a
replacement of the glass would be picked up instantly during any kind of a
radiation survey, however. Without such a survey, it is not possible to
differentiate, by appearance, lead vs. non-lead glass.

b) Adjustment knobs for the lenses seem to be slightly (x-ray) "leaky" in
some instruments, but the exposure is localized to the area of the knobs,
and therefore also to the fingers. With modern instruments where this is
done by the computer, this risk is much less and might no longer be present.
I have heard of people wrapping lead foil around their several fingers when
adjusting the knobs but I don't know if that is necessarily a "recommended"
procedure. But a general room survey would not necessarily reveal this
exposure risk.

c) Home made or radically altered commercial instruments which do not have
the "safety engineering" built into them that would otherwise be found in a
commerical instrument. I have always thought that this type of instrument
should be surveyed quite frequently because of the frequency that changes
seem to be made to the basic instrument.

Much of the motivation for those who do radiation surveys involving
commercially purchase instruments is to protect the organization from
lawsuits from former or present employees who make the claim that some
medical condition was either caused or aggrivated by their operation of
their TEM or SEM. Considering all the persons who have used both SEMs and
TEMs over the years, if there was some health issue, I would have thought
that it would have shown up clinically by now. When considering all the
risks in an EM loboratory, I have always thought that exposure to the
embedding resin chemicals would represent a far greater health risk than
operating a column instrument.

This is a very important topic and if there are other perspectives on this,
I would surely like to hear them.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 17:43:58 2004

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Peter;
I'm not sure what good OSHA is. They require records of film
badges to be kept for 30 years but don't require film badges, so guess
what my employer did? They elimininated film badges!

John Mardinly
Intel

-----Original Message-----
} From: Tomic, Peter (Peter) [mailto:ptomic-at-agere.com]
Sent: Monday, September 27, 2004 12:06 PM
To: Pat Connelly; by way of MicroscopyListserver
Cc: microscopy-at-msa.microscopy.com

Pat;

Have you tried contacting OSHA [Occupational Safety and Health
Aministration]? OSHA is a US Gov't agency. http://www.osha.gov

Peter Tomic
Agere Systems
Allentown, PA

-----Original Message-----
} From: Pat Connelly [mailto:psconnel-at-sas.upenn.edu]
Sent: Monday, September 27, 2004 1:54 PM
To: by way of MicroscopyListserver
Cc: microscopy-at-msa.microscopy.com

} Name: John H. Cross, CIH
} Organization: Lockheed Martin Space Operations
} Title-Subject: [Microscopy] [Filtered] MListserver: Occupational
} Health Aspects of
} Microscope Usage
} Question: We have a group of technicians using microscopes to solder
} electronic
} components 10-12 hours per day 6-7 days a week.
}
} Does any professional, academic, or industry organization publish
} occupational health guidelines for the use of microscopes? A sample of

} questions I need to address are:
} How often must an individual take a break from looking through a
microscope.
} Is exposure to light coming through the eyepieces a problem?
} Has anyone described ergonomically-correct work stations for
microscopists?
}
} All comments will be welcome. I particularly need objective standards
} that I can present to management to justify work practice adjustments.
}
} John Cross
Email: John H. Cross-at-lmco.com
==============
John,
I am not aware of the reference that you requested. However now that
I am in my mid-50's ,
I recently have gotten several work related physical problems.

When I am using any scope for an extended period of time my eyes fail
to focus properly
for distance. This I first noticed after nearly a whole day on my
TEM, doing a lot of
scanning for a particular cell type in sections. When I left the
building for the night I
could not see properly anything that was more than a few yards in
front of me. I realized
that this had been happening over an extended period of time but it
was not so disturbing as
it had become. I consulted my eye doctor and he said that this was
not abnormal for people
my age. The eye muscles are not as elastic as they were a few years
ago hence they do not
snap back like they used to. I need to stop "scoping" every 15
minutes or so and give my
muscles a workout by focusing on a particular object in the distance
for a short while.
This has been working great for me after I got used to the short
interruptions and my concentration was improved because of the short
distraction from what can be very boring,
when what I am looking for is difficult to find.

A second problem is the nerve that goes through my elbow. Everyone
has heard of carpel
tunnel syndrome. There is a similar area in the elbow. The test for
this is to extend the
arms straight out to the front, at shoulder height with the backs of
the hands facing
upwards.
Make a fist, flex the wrist to raise just the fist upwards. If there
is no pain, it is OK,
but if you are truly having a problem the pain can be quite intense.
I would suggest that
one contact the doctor about this. My case was already very painful
when I went to the
doctor, thinking that it would get better itself - mistake! I
received several shots into the
area over an 8 week period because I can not take oral anti-inflammatory
drugs. I also had to do exercises several times a day to strengthen the
surrounding muscles.
In the mean time I took several older mouse-pads, cut them in half,
stacked them so that
my lower arm/elbow is at least as high as my hand when using the
controls. When using the hands to solder, one may just need more
cushioning
under the elbow.
I have seen advertised scopes that claim to be more ergonomical but
at the moment
I can not recall the make.

If this is not already being done, I should expect that the fumes
from the solder should be
sucked away from the scope and go through a suitable filter so that
they are not inhaled
by the tech.

Pat Connelly
The University of Pennsylvania
Department of Biology
Philadelphia, PA 19104-6018
215-898-7145
psconnel-at-sas.upenn.edu

From MicroscopyL-request-at-ns.microscopy.com Tue Sep 28 21:56:08 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chuck's mention of the horizontal Philips 100 TEM brought to mind a story
that very likely was true. At the beginning of my microscopy career I
worked in the lab of Humberto Fernandez-Moran (of pointed filament and
diamond knife fame). Moran started using TEMS in Europe in the late 40's
including working on the earliest versions of the Philips 100 TEM. He had a
a large scar on his nose from surgery to remove a skin cancer. He blamed
the cancer on long hours with his face pressed close to the unleaded window
of the TEM and the resultant exposure to x-rays. Being rather sensitive to
this problem, he was quite careful to have later instruments tested. When I
worked in his lab we had Siemens Elmiskop 1 and 1a TEMs that tested fine.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 9/28/04 5:39 PM, "Mardinly, John" {john.mardinly-at-intel.com} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Chuck;
} Chuck;
} When I was in grad school, we purchased a used JSM-U3 through JEOL that had
} been in previous use at the GM tech center. The stage door was EXTREMELY leaky
} under normal use conditions (it was different than our other JSM-U3). I
} eventually installed a lead lining in the door.
}
} John Mardinly
} Intel
}
} -----Original Message-----
} } From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
} Sent: Tuesday, September 28, 2004 8:12 AM
} To: MICROSCOPY BB
} Subject: [Microscopy] Safety around a column instrument
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} K�rt Padari wrote:
} =====================================================
} I�m worried about my health too.
} My question: Is there any harmful radiation working with
} JEOL 100-S electron microscope?
} =====================================================
} My first exposure to an EM was in 1963, the round screen "horizontal"
} Philips 100 TEM. I was told (but don't know from my own knowledge that
} this was true) that some of these earliest models were manufactured with
} ordinary instead of lead glass. I also had contact with an early RCA TEM,
} I think it was an EMU-1 (or something close to that). Those are the only two
} EMs I have ever heard about that had inherent radiation problems and once
} the problem was recognized, retrofits were installed, taking away the
} problems. I have met operators of those early RCA instruments who
} literally did their work wearing a lead apron. My sense if that there are
} none of these old generation EMs in use any more.
}
} Instruments manufactured after that era were designed such that the mass of
} the column itself would be sufficiently massive and absorbing of x-rays,
} that raditation just could not escape during operation. True, one could
} envision that a column could be sufficiently misaligned that x-rays could
} possibly get out, but the instruments seem to be designed in a way that if
} they really were misaligned by that extent, there would be a vacuum leak and
} the high voltage would automatically shut down. Some very many years ago,
} we tried this with a JEOL 100CX and could not get the microscope
} sufficiently misaligned to allow x-rays to get out. With a JSM-U3 SEM, at
} that same time, the column would lose vacuum before it could be misaligned
} sufficiently to allow x-rays to come through.
}
}
} But there are at least several circumstances in which radiation can escape:
}
} a) Someone breaks the lead glass viewing screen of a TEM and thinking they
} are saving money, replaces the broken glass with ordinary glass. I have
} heard of this very thing happening, on occasion over the years. Such a
} replacement of the glass would be picked up instantly during any kind of a
} radiation survey, however. Without such a survey, it is not possible to
} differentiate, by appearance, lead vs. non-lead glass.
}
} b) Adjustment knobs for the lenses seem to be slightly (x-ray) "leaky" in
} some instruments, but the exposure is localized to the area of the knobs,
} and therefore also to the fingers. With modern instruments where this is
} done by the computer, this risk is much less and might no longer be present.
} I have heard of people wrapping lead foil around their several fingers when
} adjusting the knobs but I don't know if that is necessarily a "recommended"
} procedure. But a general room survey would not necessarily reveal this
} exposure risk.
}
} c) Home made or radically altered commercial instruments which do not have
} the "safety engineering" built into them that would otherwise be found in a
} commerical instrument. I have always thought that this type of instrument
} should be surveyed quite frequently because of the frequency that changes
} seem to be made to the basic instrument.
}
}
} Much of the motivation for those who do radiation surveys involving
} commercially purchase instruments is to protect the organization from
} lawsuits from former or present employees who make the claim that some
} medical condition was either caused or aggrivated by their operation of
} their TEM or SEM. Considering all the persons who have used both SEMs and
} TEMs over the years, if there was some health issue, I would have thought
} that it would have shown up clinically by now. When considering all the
} risks in an EM loboratory, I have always thought that exposure to the
} embedding resin chemicals would represent a far greater health risk than
} operating a column instrument.
}
} This is a very important topic and if there are other perspectives on this,
} I would surely like to hear them.
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================
}
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 29 01:00:02 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As has been mentioned previously on this listserver, at first nearly every
state, if not all of them, required regular surveys of EMs. This was
sparked by at least one manufacturer that released some TEMs with
non-leaded glass (problems mainly restricted to the facial area) and I
believe at least one other instance of TEM camera assemblies that leaked (a
number of early TEM operators took to clipping their film badges over their
genitals). Over the years, most states have dropped this requirement as
few surveys ever detect anything. However, a good knowledge of the
potential radiation and the detecting methods used is really required.

Any change in radiation leakage in an EM over time is probably an
indication that either something has been improperly changed in the EM or
the instruments being used to detect a problem have changed.

In the first case, the basic design of a modern EM (and by that I mean
anything produced in the last 30 years) makes them inherently safe in most
respects. The standard design of shrouding electromagnetic lenses in soft
iron (ferromagnetics) in order to use those shrouds to focus the fields in
narrow spaces means that the majority of the column is well shielded. In
areas where these lenses aren't used, the outer structures are very thick
metal just to support the electron gun or upper lenses and keep them in
alignment. Where these sections are mated, they normally have close
fitting, concentric overlaps for alignment. In other sealing areas, such
as vacuum manifolds, gauges, port covers and door seals, sufficient overlap
is provided to prevent a direct path for x-rays.

For example, a flat door with an o-ring sealing up against flat metal
chamber in an SEM generally has a half an inch to an inch of overlap. Yes,
x-rays will leak, but due to the repeated, zigzag path they would have to
take to get out, you won't see primary x-rays. Instead, you will get the
result of multiple fluorescence events, with each generation producing
x-rays of lower energy and fewer photons that can make it out. There
could, however, be structures within the chamber that are always, or
occasionally, in the right position to provide a fluorescence to the
primary sample x-rays with a portion going directly out between the door
and the chamber, through the o-ring.

That brings us to the equipment used to detect these leaks. Geiger tubes
of various shapes and sizes have been the standard over the years. There
are two general varieties - compensated and uncompensated. The
uncompensated tube is a bare tube. Because of the design, the
uncompensated Geiger tube is extremely sensitive to x-rays, the lower the
energy the more it reports. As long as some x-rays can penetrate the outer
glass tube, or cause a secondary fluorescence in it, the lower energy
x-rays stand a much better chance of ionizing the gas within.

Because a wide range of radiation detection is desired for most Geiger
counters, various means are used to 'compensate' them. This basically
means shielding them from lower energy x-rays so that they aren't swamped
by them and thus higher energy x-rays and gamma rays can be more
effectively detected. Other types of radiation detectors can have a
response similar to the uncompensated Geiger tube - they can be far more
sensitive to low energy x-rays than other radiation, but they are often
calibrated to high energy x-rays or gamma rays (the most interesting form
of radiation detection for many uses) and perhaps compensated in some way.

Leaving the technical behind, a change over time in the x-ray emissions of
an EM could also be the result of a change in instrumentation to detect it.
If the detector doesn't have a linear calibration that includes the low
x-ray energies, then it may produce results on an EM that don't represent
reality. Since most survey instruments can't differentiate radiation
sources by energy, appropriate adjustment has to be made to their readings.
An SEM running at 30KV won't leak 30KV x-rays, and if that assumption is
made the reading will be off. More than likely any emitted radiation would
be an order of magnitude or more lower in energy. At those energies, the
survey meter would likely have a much different sensitivity, perhaps
several orders of magnitude.

In regards to vacuum integrity, generally an instrument column misaligned
enough to allow x-ray leakage from the column would not be capable of
maintaining a vacuum and thus the instrument would itself prevent the
application of the electron beam. Avoiding the issue of properly operating
vacuum interlocks, I confess I can think of an instrument or two that could
have such misalignments and still operate. These would be visibly obvious
to anyone familiar with the instrument and extremely unlikely to occur even
from the most inexperienced service personnel. Yet, the possibility
exists.

User modification of an instrument is one I watch for carefully. I have
had customers who have done things like replace a sample chamber metal port
cover with a Plexiglas panel so they could peer in and see the sample
positioning. Of course, they would fashion a light tight cover to prevent
swamping of the secondary electron detector in operation, but it would
usually be of a thin aluminum construction. Great, we now have an aluminum
fluorescence source external to the chamber. Don't try this at home,
kiddies.

Localized, point sources such as lens adjustment knobs present little
problem. First, remember that radiation rates fall off as the inverse
square of the distance. If you measure a dose rate at 5mm from the
external exit, at 10mm it is 1/4 as strong, at 20mm 1/16 the strength and
at 40mm (just 1.6") 1/64 as strong.

You also have to consider the extent of the exposure. We probably all
agree that we'd like to minimize exposure to radiation as much as possible,
but what areas of the body are exposed have a large effect on any probable
future problems. While not fully understood, radiation effects are
generally agreed to affect different parts of the body to differing
extents. At the low end of things is exposure of peripheral areas - hands,
arms, feet and legs. These areas don't contain the essential organs that
the thorax does, don't have the faster regenerating cells or the high blood
supply that can support rapid cellular mutations. While there are always
exceptions, you rarely hear of cancers that originate in the fingers or
toes, arms or legs.

My own personal feeling is that Chuck is right, you stand a much greater
chance of long term damage from the various chemicals used in sample
preparation, not to mention common solvents and the samples themselves,
used in an EM lab than you do from possible radiation from an EM.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com

On Tuesday, September 28, 2004 10:12 AM, Garber, Charles A.
[SMTP:cgarber-at-2spi.com] wrote:
}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
-------
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Kart Padari wrote:
} =====================================================
} I?m worried about my health too.
} My question: Is there any harmful radiation working with
} JEOL 100-S electron microscope?
} =====================================================
} My first exposure to an EM was in 1963, the round screen "horizontal"
} Philips 100 TEM. I was told (but don't know from my own knowledge that
} this was true) that some of these earliest models were manufactured with
} ordinary instead of lead glass. I also had contact with an early RCA
TEM,
} I think it was an EMU-1 (or something close to that). Those are the only
two
} EMs I have ever heard about that had inherent radiation problems and once
} the problem was recognized, retrofits were installed, taking away the
} problems. I have met operators of those early RCA instruments who
} literally did their work wearing a lead apron. My sense if that there
are
} none of these old generation EMs in use any more.
}
} Instruments manufactured after that era were designed such that the mass
of
} the column itself would be sufficiently massive and absorbing of x-rays,
} that raditation just could not escape during operation. True, one could
} envision that a column could be sufficiently misaligned that x-rays could
} possibly get out, but the instruments seem to be designed in a way that
if
} they really were misaligned by that extent, there would be a vacuum leak
and
} the high voltage would automatically shut down. Some very many years
ago,
} we tried this with a JEOL 100CX and could not get the microscope
} sufficiently misaligned to allow x-rays to get out. With a JSM-U3 SEM,
at
} that same time, the column would lose vacuum before it could be
misaligned
} sufficiently to allow x-rays to come through.
}
}
} But there are at least several circumstances in which radiation can
escape:
}
} a) Someone breaks the lead glass viewing screen of a TEM and thinking
they
} are saving money, replaces the broken glass with ordinary glass. I have
} heard of this very thing happening, on occasion over the years. Such a
} replacement of the glass would be picked up instantly during any kind of
a
} radiation survey, however. Without such a survey, it is not possible to
} differentiate, by appearance, lead vs. non-lead glass.
}
} b) Adjustment knobs for the lenses seem to be slightly (x-ray) "leaky"
in
} some instruments, but the exposure is localized to the area of the knobs,
} and therefore also to the fingers. With modern instruments where this is
} done by the computer, this risk is much less and might no longer be
present.
} I have heard of people wrapping lead foil around their several fingers
when
} adjusting the knobs but I don't know if that is necessarily a
"recommended"
} procedure. But a general room survey would not necessarily reveal this
} exposure risk.
}
} c) Home made or radically altered commercial instruments which do not
have
} the "safety engineering" built into them that would otherwise be found in
a
} commerical instrument. I have always thought that this type of
instrument
} should be surveyed quite frequently because of the frequency that changes
} seem to be made to the basic instrument.
}
}
} Much of the motivation for those who do radiation surveys involving
} commercially purchase instruments is to protect the organization from
} lawsuits from former or present employees who make the claim that some
} medical condition was either caused or aggrivated by their operation of
} their TEM or SEM. Considering all the persons who have used both SEMs
and
} TEMs over the years, if there was some health issue, I would have thought
} that it would have shown up clinically by now. When considering all
the
} risks in an EM loboratory, I have always thought that exposure to the
} embedding resin chemicals would represent a far greater health risk than
} operating a column instrument.
}
} This is a very important topic and if there are other perspectives on
this,
} I would surely like to hear them.
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================
}
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 29 11:52:45 2004

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Short Course and Workshop Announcement
University of Missouri - Columbia
(originally scheduled for August 16-18, 2004 was postponed due to Dr.
Russ' illness)

The Electron Microscopy Core Facility is hosting a 3-day Short Course
and Workshop on Computer-Assisted Image Analysis and Measurement
taught by Dr. John C. Russ on November 9=11, 2004. This popular
course is intended to familiarize users of image analysis equipment
with the fundamental principles and methods available to obtain
meaningful results, and to educate laboratory supervisors or research
professionals seeking to learn how to use such methods in their
applications. The techniques are applicable to fields ranging from
materials, geological and biological/medical research to food
technology and manufacturing quality control.

The course relies heavily on tightly coupled lectures and hands-on
experience with the various techniques. The laboratory includes a
wide variety of image analysis methods designed to cover the range of
approaches and tools, and a detailed set of practical instructions to
enable their use with a minimal learning curve. No specific
background is assumed, although users should already be familiar with
microscopy or other imaging technology, and the techniques required
to obtain the images to be measured. Many of the examples used in the
course involve light or electron microscope images, but students are
invited to bring their own most interesting images (TIFF files) for
discussion and analysis.

Image analysis and measurement methods are used in a broad range of
applications and are usually concerned with extracting a few
numerical values, such as the number, size, shape or location of
objects from the image. In other cases, global structural parameters
such as measures of the volume and surface of structures present are
of interest. These measurements may require image processing to
correct defects, feature enhancement, comparison of multiple images,
object recognition, or other steps. Ultimately, the image is reduced
to just the features of interest. Measurements on these individual
features, or on the image as a whole, must then be obtained and
interpreted in a proper stereological context to obtain useful data
about the objects. Statistical interpretation of the data allows
comparisons of different populations, understanding of distribution
plots, and other inferences about the original objects. Structural
modeling and geometric probability can be used to develop models for
this interpretation.

The course will have an enrollment limit of 20, and there are ~10
spots still open. More information including a registration form can
be found at http://www.emc.missouri.edu, or by contacting Lou Ross at
573.882.4777 or rosslm-at-missouri.edu.
--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 29 11:59:53 2004

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Hi everyone,

Can you help my colleague, Milos Kalab, to solve his problem. His question is posted below. Thank you.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/T�l�phone: 613-759-1638
Facsimile/T�l�copieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

My objective is to obtain SEM images of stainless steel and other surfaces used in food processing. For this reason I wish to replicate small parts of the surfaces and examine the negative replicas. Advice on materials and procedures which would reproduce details down to 1 micrometer would be appreciated. (I am aware of Ted Pella and SPI cellulose acetate replication materials for coarser surfaces and I wonder why I have not been successful.)

Thank you.

Milos Kalab

Agriculture and Agri-Food Canada in Ottawa

kalabm-at-agr.gc.ca

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 29 12:23:42 2004

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Dear Listserver,

Is any one using or know of a consumer camera with full computer control
and live video preview mounted on an optical microscope?

Peggy Miller
UTHSCSA Ophthalmology
Lions Sight Research Center
7703 Floyd Curl Drive MC6230
San Antonio, Texas 78229-3900
PH: (210)567-8460
FAX:(210)567-8413

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 29 16:11:25 2004

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We routinely use acetate replicas from machined metal surfaces that resolve
to the micron level.

Can you describe the procedure used by Milos? Perhaps it differs from what
we do.

Regards,
Woody

-----Original Message-----
} From: Yang, Ann-Fook [mailto:YANGA-at-agr.gc.ca]
Sent: Wednesday, September 29, 2004 1:01 PM
To: microscopy-at-microscopy.com

Hi everyone,

Can you help my colleague, Milos Kalab, to solve his problem. His question
is posted below. Thank you.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/T�l�phone: 613-759-1638
Facsimile/T�l�copieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

My objective is to obtain SEM images of stainless steel and other surfaces
used in food processing. For this reason I wish to replicate small parts of
the surfaces and examine the negative replicas. Advice on materials and
procedures which would reproduce details down to 1 micrometer would be
appreciated. (I am aware of Ted Pella and SPI cellulose acetate replication
materials for coarser surfaces and I wonder why I have not been successful.)

Thank you.

Milos Kalab

Agriculture and Agri-Food Canada in Ottawa

kalabm-at-agr.gc.ca

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 29 17:10:08 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mark.evans-at-uvm.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 29, 2004 at 15:53:40
---------------------------------------------------------------------------

Email: mark.evans-at-uvm.edu
Name: Mark Evans

Organization: University of Vermont

Title-Subject: [Microscopy] [Filtered] MListserver: Isoamyl acetate and critical point drying

Question: Some protocols for sem indicate that following ethanol series dehydration samples should be soaked in isoamyl acetate and that this media should also be used for critical point drying. Can anyone tell me what the theory is behind using isoamyl acetate and what benefit, if any, there is over leaving samples in 100% ethanol prior to the critical point drying?
Thanks,
Mark

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Sep 29 21:34:39 2004

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Milos Kalab asks:
====================================================
My objective is to obtain SEM images of stainless steel and other surfaces
used in food processing. For this reason I wish to replicate small parts of
the surfaces and examine the negative replicas. Advice on materials and
procedures which would reproduce details down to 1 micrometer would be
appreciated. (I am aware of Ted Pella and SPI cellulose acetate replication
materials for coarser surfaces and I wonder why I have not been successful.)
====================================================
If you are talking about pretty smooth surfaces, there is no reason that I
can see why either a) the cellulose acetate system should not "work" or b)
you could also try what we at SPI Supplies call the SPI Wet Replica Kit,
which is a very rapidly curing silicone system.

You did not say what your basis was for it "not working" but I will assume
that you just did not "see" anything when in fact you expected to be seeing
something. There could be several possible reasons for this:

a) there is some kind of organic contamination coating the surface, so if
you solvent washed the surface (or plasma etched it if it was not too large
to get into a plasma etcher) you might "uncover" what you wanted to be
seeing and/or

b) work with a more dilute solution of the cellulose acetate or

c) there is structure there but you might do better seeing it by Pt/C
shadowing the cellulose acetate (negative) replica, dissolving away the
plastic, picking up the replica on a TEM grid and viewing the replica in a
TEM instead of an SEM. It is possible that the topographical variation of
your structure is insufficient to give the level of contrast needed to be
seen by SEM.

The silicone system might be easier to work with, especially if you wanted
to return to the same identical area, if you wanted to be following the same
area as a function of time (e.g. as a function of use if you are doing wear
studies). But the same comments about making sure the surface is "clean"
and properly "prepared" would apply, otherwise you will just be replicating
the surface of the contamination or residues. The silicone system would
work only for SEM examination, not TEM.

Disclaimer: SPI Supplies offers both cellulose acetate replicating tapes
and sheets and also the SPI Wet Replica kit as described on the website
given below.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 02:28:47 2004

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I have not used amyl acetate for CPD since my first CPD run almost 30
years ago.
The head of department was incensed by the strong pear/banana smell
and banned it as antisocial.
In those days nobody seemed to worry about its toxicity. Amyl acetate
also damages
the rubber seals of the CPD faster than other solvents.

The objective is to fully replace the dehydrating solvent with liquid
CO2.
Amyl acetate is more soluble in liquid CO2 than the more polar
ethanol, but
many people nevertheless use ethanol routinely and obtain great
results, especially if the
specimens are small, thin, and unenclosed by cuticle (cultured cells
on coverslips, for example).
If the specimens are large chunks of plant material it would be better
to work with acetone (propanone)
as both dehydrating and intermediate solvent.

Dr. Chris Jeffree
University of Edinburgh

----- Original Message -----
} From: "by way of MicroscopyListserver" {mark.evans-at-uvm.edu}
To: {microscopy-at-microscopy.com}
Sent: Wednesday, September 29, 2004 11:12 PM

Peggy

most of the recent Canon Powershot range allow for control and previewing of the camera from a computer via the USB port. The A80 (recently superseded by the A95) and the A95 also have a screen which can be rotated to a useful angle for viewing when the camera is on the top of a microscope. One disadvantage is that they only produce JPEG images (not RAW or TIFF).
You could check some of the specifications at:
http://consumer.usa.canon.com/ir/controller?act=ProductCatIndexAct&fcategoryid=113

A more upmarket Canon would be the Powershot G6 (this does JPEG and RAW format I believe) - see website below for a review which includes detail of the computer control facilities:
http://www.dcresource.com/reviews/canon/powershot_g6-review/index.shtml

I would assume that some Nikons may have the facilities that you want but I have no personal experience of them - just my trusty little A80.

Certainly there are are microscope and camera adaptors to enable attachment to light microscopes for Nikon and Canon digital cameras - just do a web search.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
} From: "Miller, Margaret M" {MILLERMM-at-uthscsa.edu}

Hi, Peggy!

We do quite a lot of LM digital imaging these days. Through Nikon we had
purchased the Photometrics Cool Snap cf camera to place atop an Olympus BH-2
light microscope. The software program we're using is called Metavue; it
allows you to view live and make changes to lighting and such before saving.
The best part is being able to make much smaller files by making duplicate
images and saving into TIFF. It also allows you to put in micron bars after
calibrating your scope and camera system together. Afterwards, the
duplicated images are able to be used in Photoshop or Power Point.

Good luck in your search!

*Disclaimer--I do not work for, or receive any compensation from, Nikon or
the makers of Metavue. We are just satisfied with the setup.

Donna R. Clarkson, HT (ASCP)
Northrop Grumman Information Technology
for U S Army Medical Research Detachment
at Brooks City-Base
Phone (210) 536-1416
FAX (210) 536-1449
e-mail donna.clarkson-at-brooks.af.mil
"Our Army at War--Relevant and Ready"

-----Original Message-----
} From: Miller, Margaret M [mailto:MILLERMM-at-uthscsa.edu]
Sent: Wednesday, September 29, 2004 12:25 PM
To: Microscopy-at-microscopy.com

Dear Listserver,

Is any one using or know of a consumer camera with full computer control
and live video preview mounted on an optical microscope?

Peggy Miller
UTHSCSA Ophthalmology
Lions Sight Research Center
7703 Floyd Curl Drive MC6230
San Antonio, Texas 78229-3900
PH: (210)567-8460
FAX:(210)567-8413

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 09:04:36 2004

Contents Retrieved from Microscopy Listserver Archives
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Amyl acetate is supposed to be more miscible in lqCO2 than is EtOH. I
have also heard that it's supposed to be a suspected carcinogen, but
I don't have any references on that. One reason I was given for using
Amyl acetate when I was learning EM Back When was that it smells of
bananas, and therefore it can be easily known when it's all been
flushed out.
I've never used A. acetate, just EtOH, and I get fine results. I may
have to purge more than I would if I used A. acetate, but not much.
And the EtOH has plenty of odor, so it's also easy to tell when the
bulk is gone by smell.
But it's the Aac or EtOH *in* the samples that has to be gotten rid
of, not the bulk fluid, and that's too small a volume to smell anyway.
Phil

} Email: mark.evans-at-uvm.edu
} Name: Mark Evans
}
} Organization: University of Vermont
}
} Title-Subject: [Microscopy] [Filtered] MListserver: Isoamyl acetate
} and critical point drying
}
} Question: Some protocols for sem indicate that following ethanol
} series dehydration samples should be soaked in isoamyl acetate and
} that this media should also be used for critical point drying. Can
} anyone tell me what the theory is behind using isoamyl acetate and
} what benefit, if any, there is over leaving samples in 100% ethanol
} prior to the critical point drying?
} Thanks,
} Mark
}
} ---------------------------------------------------------------------------

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 09:07:27 2004

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Greetings Margaret,
I don't know of any "consumer grade" cameras (the kind you can get at
BestBuy) with real time focusing and robust computer control. We had an
older Kodak DC210 "micro-imaging package" and it was, and still is,
quite possibly the worst ccd solution marketed for microscopy that I
have ever seen. The little Pixellink cameras for microscopy are fine
for routine brightfield work but they cost about $2k (the Kodak system
was about the same amount several years ago). A favorite among amateur
microscopists is the Nikon CoolPix (google nikon coolpix microscopy),
but I don't know of a real time computer control interface for it. In
order to use such a camera on a microscope you either have to dimantle
the camera to expose the bare ccd, then make some sort of c-mount
adapter, or you will have to get a hold of a special optical coupler
with a lens system to project the image on the ccd correctly through the
camera lens. Actually I've heard that you can take a snapshot directly
through the eyepiece if you hold the camera just right. I highly
recommend a camera designed for microscopy if you don't want to look
through the eyepieces or don't like judging image quality from a 2 inch
low-res lcd.
-Karl

--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 10:08:14 2004

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The following is the agenda for the Fall MATCSEM and Midwest Society meeting. For those planning on attending, please email me for parking permits:

wcarmichael-at-matcmadison.edu

Annual Fall MATCEM / MMMS Microscopy Meeting

Friday, October 15 at Madison Area Technical College, Truax campus

8:45 - 9:30 Meeting registration (Coffee, tea, juice, donuts and bagels will be provided)

9:30 - 9:45 Welcome, Introductions and Business information

9:45 - 10:30 Food Microstructure: Industry Perspectives
By Randy Brandsma of Schreiber Foods, Inc.
Randy is currently a research scientist with Schreiber Foods Inc. of Green Bay WI.
After receiving his MS in Dairy Science and working as a QA manager for Davisco International he earned his PhD from Cornell University with focus on membrane filtration for cheese manufacturing. He is currently using Confocal and SEM in his work to understand the microstructure of cheese and its effects on performance.

10:30 - 12:00 Forensic Microscopical Analysis of Food, Beverages and Containers from Top to Bottom.
By Richard E. Bisbing and Elaine F. Schumacher of McCrone Associates, Inc.

Richard is the Executive Vice President, managing the technical operations group and overseeing all services at McCrone Associates. Prior to joining McCrone Richard was involved in trace evidence work for the Michigan state police and Dept. of public health. He continues to consult on analytical light microscopy and forensic sciences.

Elaine is a Senior Research Scientist and utilizes TEM techniques such as high resolution imaging, electron diffraction, x-ray microanalysis and EELS to characterize a wide variety of industrial materials and problems. Prior to McCrone Elaine worked at UOP Research Center developing catalysts and process technology for the refining industry.

Lunch - A box lunch will be provided courtesy of the MATC vendors

1:15 - 2:30 How Measurements in the nm to �m Size Range Help in Polymer Product Development by Bodan Ma and Sunil Jayasuriya of SC Johnson Polymer

Sunil is the Technical Manager for Polymer Characterization and Applications Research group. He earned his PhD in Physical Chemistry from the Univ. of Bristol, UK. Sunil has worked on applications of surface chemistry and colloid science for printing, personal care, and package coating industries.

Bodan is the Technical Manager for Coatings and has extensive experience in the structure-property relationship of polymeric materials, such as adhesives and coatings. He holds a PhD in Polymer Sciences from Univ. of Massachusetts, Amherst and holds 8 US patents in formulation of new materials.

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 11:10:47 2004

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The very old Sorvall Critical Point Dryer came with a spare parts box
that had special
O-Rings that needed to be used if amyl acetate was to be used. The
directions did not
give any reason for using it. I have usually used Acetone for
dehydrations and a freshly
opened bottle of Mallinckrodt #2440 acetone for the final exchange
before the drying.

Many years ago an investigator insisted on using amyl acetate with
his sample and he
ran the operation himself. He later admitted that similar samples
that I ran in acetone
looked better.

If one uses amyl acetate in the critical point dryer it is extremely
necessary for the
amyl acetate to be properly vented into a hood (acetone also). If
the amyl acetate of
this volume gets into the room it can make one behave strangely.
My co-workers thought that I was drunk! I did not make that mistake again.
Pat Connelly
The University of Pennsylvania
Department of Biology
Philadelphia, PA 19104-6018
215-898-7145
psconnel-at-sas.upenn.edu
===========================
} I have not used amyl acetate for CPD since my first CPD run almost
} 30 years ago.
} The head of department was incensed by the strong pear/banana smell
} and banned it as
} antisocial.In those days nobody seemed to worry about its toxicity.
} Amyl acetate also
} damages the rubber seals of the CPD faster than other solvents.
}
} The objective is to fully replace the dehydrating solvent with liquid CO2.
} Amyl acetate is more soluble in liquid CO2 than the more polar ethanol, but
} many people nevertheless use ethanol routinely and obtain great
} results, especially if
} the specimens are small, thin, and unenclosed by cuticle (cultured
} cells on coverslips,
} for example). If the specimens are large chunks of plant material it
} would be better to
} work with acetone (propanone) as both dehydrating and intermediate solvent.
}
} Dr. Chris Jeffree {c.jeffree-at-ed.ac.uk}
} University of Edinburgh
============================
} } Email: mark.evans-at-uvm.edu
} } Name: Mark Evans
} } Organization: University of Vermont
} } Title-Subject: [Microscopy] Isoamyl acetate and critical point drying
} }
} } Question: Some protocols for sem indicate that following ethanol
} } series dehydration samples should be soaked in isoamyl acetate and
} } that this media should also be used for critical point drying. Can
} } anyone tell me what the theory is behind using isoamyl acetate and
} } what benefit, if any, there is over leaving samples in 100% ethanol
} } prior to the critical point drying?
} } Thanks,
} } Mark
} } ---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 12:36:11 2004

Contents Retrieved from Microscopy Listserver Archives
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We have been doing replicating products since the 1950s, so if you wish to
discuss replication please call Mike Bouchard at 1-800-451-3406 and he'll be
glad to provide assistance on replicating. Mike has many years of
experience in this area.

Thanks,
Deb Sicard

Disclaimer: Ladd has been a supplier of EM products, including replicating
materials, for more than 50 years

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: ladres-at-att.net

----- Original Message -----
} From: "White, Woody N." {nwwhite-at-bwxt.com}
To: "'Yang, Ann-Fook'" {YANGA-at-agr.gc.ca} ; {microscopy-at-microscopy.com}
Sent: Wednesday, September 29, 2004 5:06 PM

We routinely use acetate replicas from machined metal surfaces that resolve
to the micron level.

Can you describe the procedure used by Milos? Perhaps it differs from what
we do.

Regards,
Woody

-----Original Message-----
} From: Yang, Ann-Fook [mailto:YANGA-at-agr.gc.ca]
Sent: Wednesday, September 29, 2004 1:01 PM
To: microscopy-at-microscopy.com

Hi everyone,

Can you help my colleague, Milos Kalab, to solve his problem. His question
is posted below. Thank you.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/T�l�phone: 613-759-1638
Facsimile/T�l�copieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

My objective is to obtain SEM images of stainless steel and other surfaces
used in food processing. For this reason I wish to replicate small parts of
the surfaces and examine the negative replicas. Advice on materials and
procedures which would reproduce details down to 1 micrometer would be
appreciated. (I am aware of Ted Pella and SPI cellulose acetate replication
materials for coarser surfaces and I wonder why I have not been successful.)

Thank you.

Milos Kalab

Agriculture and Agri-Food Canada in Ottawa

kalabm-at-agr.gc.ca

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 15:02:08 2004

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I can offer only the comments of an experimenting amateur, with a Nikon Coolpix 990. I am using GNU/Linux as an operating system, and have had a modicum of success with setting up several programs to manipulate the camera's controls. It's exciting enough to encourage me to do further experiments with a newer model of Coolpix.

I take photos through the eyepiece, using a relatively inexpensive coupler that is merely a clamp to hold the camera onto the eyepiece. Several companies manufacture these, as well as optical couplers (so-called relay lenses) which take the place of an eyepiece. (I don't understand the difference between relay lenses and the projection lenses of the past).

I really like that the coolpix can be monitored in real time through the TV, which works wonders for me, as a teacher, in teaching about microscopical realms without having student microscopes at our school. Using some tips for setting up this camera for microscopy (more than one set of which I found on the Inet) I find it relatively easy to take at least snapshots. With a cable release, and a bright enough light---perhaps I will finally get around to setting up a strobe---the results promise to be able to freeze motion even of cilia.

A promising suggestion was to use a video capture card to move the video image to the computer screen while using software to remotely fire the camera. I don't know whether this is possible, but I hope to try it. I can see why the Coolpix 990 has received so much attention from the microscopical community. It is available relatively cheaply on ebay, too, so perhaps this could serve as the basis for some experiments for you, too.

I am doubly encouraged at how well the camera is supported by a GIMP (Gnu Image Manipulation Program---a photoshop like program) plugin. Better than any other software, so far, I have found it easy to even monitor the image in *pretty much* real time and snap on demand, at which point the image is immediately available for cropping and editing. The program is free (in terms of your being able to share copies---in terms, in other words, of freeDOM, as well as free BEER freedom). This, again, encourages my further experimentation. I am considering the upcoming Nikon Coolpix 8800 which has vibration reduction technology included!

Take my comments with the appropriate grain of salt.

Alan Davis
Kagman High School
Saipan, Northern Mariana Islands
aedavis-at-eccomm.com

On Thu, 30 Sep 2004 08:02:31 -0500
Clarkson Donna R Contr USAMRD/MCMR {donna.clarkson-at-brooks.af.mil} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi, Peggy!
}
} We do quite a lot of LM digital imaging these days. Through Nikon we had
} purchased the Photometrics Cool Snap cf camera to place atop an Olympus BH-2
} light microscope. The software program we're using is called Metavue; it
} allows you to view live and make changes to lighting and such before saving.
} The best part is being able to make much smaller files by making duplicate
} images and saving into TIFF. It also allows you to put in micron bars after
} calibrating your scope and camera system together. Afterwards, the
} duplicated images are able to be used in Photoshop or Power Point.
}
} Good luck in your search!
}
} *Disclaimer--I do not work for, or receive any compensation from, Nikon or
} the makers of Metavue. We are just satisfied with the setup.
}
} Donna R. Clarkson, HT (ASCP)
} Northrop Grumman Information Technology
} for U S Army Medical Research Detachment
} at Brooks City-Base
} Phone (210) 536-1416
} FAX (210) 536-1449
} e-mail donna.clarkson-at-brooks.af.mil
} "Our Army at War--Relevant and Ready"
}
}
}
} -----Original Message-----
} } From: Miller, Margaret M [mailto:MILLERMM-at-uthscsa.edu]
} Sent: Wednesday, September 29, 2004 12:25 PM
} To: Microscopy-at-microscopy.com
} Subject: [Microscopy] LM digital imaging
}
}
}
}
} ----------------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
} ---
}
} Dear Listserver,
}
} Is any one using or know of a consumer camera with full computer control
} and live video preview mounted on an optical microscope?
}
} Peggy Miller
} UTHSCSA Ophthalmology
} Lions Sight Research Center
} 7703 Floyd Curl Drive MC6230
} San Antonio, Texas 78229-3900
} PH: (210)567-8460
} FAX:(210)567-8413
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 16:51:56 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello, All-

Last year a couple of students from BYU Hawaii were interested in looking
for copper in the mitochondria of yeast by EELS and/or ESI, using our LEO
912 EFTEM. The first (but by no means the only) stumbling block was
getting decent ultrastructure, or at least good enough that they could
identify the mitochondria, and good enough infiltration that 25-30 nm
sections could be obtained.

I do not have any experience with yeast and so I am open to any and all
hints, tips, and suggestions!

If this works out, I invite you all to come to see the results at M&M 2005
in Honolulu next August.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 17:57:15 2004

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After years of trying desperately to get one of our microtomes
working, I'm thinking of changing the company that provides
service on our instruments (2 Reichert Ultracut E and 2 Leica
UCT/FCS). Does anyone in the NorthEast know of someone
capable of providing service in Connecticut? I'm especially
interested in someone with good experience with the
Leica instruments.
Thank you

Marc
--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

From MicroscopyL-request-at-ns.microscopy.com Thu Sep 30 21:23:11 2004

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John:

See http://http://www.math.ualberta.ca/imaging/
There, one might find these remarks:

"... newer cameras (Coolpix 995/775 and beyond) can only be controlled via a serial cable"

There's more to all this than I understand.

Alan

On Thu, 30 Sep 2004 20:58:25 -0400
John Twilley {jtwilley-at-sprynet.com} wrote:

} Dear Alan,
}
} Do I understand you correctly that the 990 has some input for control by a PC? Is this true of the 995 as well? Are you writing this control routine yourself?
}
} John Twilley
}
} Alan E. Davis wrote:
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} } I can offer only the comments of an experimenting amateur, with a Nikon Coolpix 990. I am using GNU/Linux as an operating system, and have had a modicum of success with setting up several programs to manipulate the camera's controls. It's exciting enough to encourage me to do further experiments with a newer model of Coolpix.
} }
} } I take photos through the eyepiece, using a relatively inexpensive coupler that is merely a clamp to hold the camera onto the eyepiece. Several companies manufacture these, as well as optical couplers (so-called relay lenses) which take the place of an eyepiece. (I don't understand the difference between relay lenses and the projection lenses of the past).
} }
} } I really like that the coolpix can be monitored in real time through the TV, which works wonders for me, as a teacher, in teaching about microscopical realms without having student microscopes at our school. Using some tips for setting up this camera for microscopy (more than one set of which I found on the Inet) I find it relatively easy to take at least snapshots. With a cable release, and a bright enough light---perhaps I will finally get around to setting up a strobe---the results promise to be able to freeze motion even of cilia.
} }
} } A promising suggestion was to use a video capture card to move the video image to the computer screen while using software to remotely fire the camera. I don't know whether this is possible, but I hope to try it. I can see why the Coolpix 990 has received so much attention from the microscopical community. It is available relatively cheaply on ebay, too, so perhaps this could serve as the basis for some experiments for you, too.
} }
} } I am doubly encouraged at how well the camera is supported by a GIMP (Gnu Image Manipulation Program---a photoshop like program) plugin. Better than any other software, so far, I have found it easy to even monitor the image in *pretty much* real time and snap on demand, at which point the image is immediately available for cropping and editing. The program is free (in terms of your being able to share copies---in terms, in other words, of freeDOM, as well as free BEER freedom). This, again, encourages my further experimentation. I am considering the upcoming Nikon Coolpix 8800 which has vibration reduction technology included!
} }
} } Take my comments with the appropriate grain of salt.
} }
} } Alan Davis
} } Kagman High School
} } Saipan, Northern Mariana Islands
} } aedavis-at-eccomm.com
} }
} } On Thu, 30 Sep 2004 08:02:31 -0500
} } Clarkson Donna R Contr USAMRD/MCMR {donna.clarkson-at-brooks.af.mil} wrote:
} }
} } }
} } }
} } } ------------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -------------------------------------------------------------------------------
} } }
} } } Hi, Peggy!
} } }
} } } We do quite a lot of LM digital imaging these days. Through Nikon we had
} } } purchased the Photometrics Cool Snap cf camera to place atop an Olympus BH-2
} } } light microscope. The software program we're using is called Metavue; it
} } } allows you to view live and make changes to lighting and such before saving.
} } } The best part is being able to make much smaller files by making duplicate
} } } images and saving into TIFF. It also allows you to put in micron bars after
} } } calibrating your scope and camera system together. Afterwards, the
} } } duplicated images are able to be used in Photoshop or Power Point.
} } }
} } } Good luck in your search!
} } }
} } } *Disclaimer--I do not work for, or receive any compensation from, Nikon or
} } } the makers of Metavue. We are just satisfied with the setup.
} } }
} } } Donna R. Clarkson, HT (ASCP)
} } } Northrop Grumman Information Technology
} } } for U S Army Medical Research Detachment
} } } at Brooks City-Base
} } } Phone (210) 536-1416
} } } FAX (210) 536-1449
} } } e-mail donna.clarkson-at-brooks.af.mil
} } } "Our Army at War--Relevant and Ready"
} } }
} } }
} } }
} } } -----Original Message-----
} } } } From: Miller, Margaret M [mailto:MILLERMM-at-uthscsa.edu]
} } } Sent: Wednesday, September 29, 2004 12:25 PM
} } } To: Microscopy-at-microscopy.com
} } } Subject: [Microscopy] LM digital imaging
} } }
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
} } } --
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.msa.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } } ---
} } }
} } } Dear Listserver,
} } }
} } } Is any one using or know of a consumer camera with full computer control
} } } and live video preview mounted on an optical microscope?
} } }
} } } Peggy Miller
} } } UTHSCSA Ophthalmology
} } } Lions Sight Research Center
} } } 7703 Floyd Curl Drive MC6230
} } } San Antonio, Texas 78229-3900
} } } PH: (210)567-8460
} } } FAX:(210)567-8413
} } }
} } }
} } }
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 1 07:37:03 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tek-Net in Lakewood, NJ
732-905-5530

Geoff

Marc Pypaert wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} After years of trying desperately to get one of our microtomes
} working, I'm thinking of changing the company that provides
} service on our instruments (2 Reichert Ultracut E and 2 Leica
} UCT/FCS). Does anyone in the NorthEast know of someone
} capable of providing service in Connecticut? I'm especially
} interested in someone with good experience with the
} Leica instruments.
} Thank you
}
} Marc
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 1 08:52:21 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marc, et al,

I am also interested in getting a repairperson for my Ultracut E. It really
needs an overhaul.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

-----Original Message-----
} From: Marc Pypaert [mailto:marc.pypaert-at-yale.edu]
Sent: Thursday, September 30, 2004 6:58 PM
To: 'Microscopy-at-MSA.Microscopy.Com'

After years of trying desperately to get one of our microtomes
working, I'm thinking of changing the company that provides
service on our instruments (2 Reichert Ultracut E and 2 Leica
UCT/FCS). Does anyone in the NorthEast know of someone
capable of providing service in Connecticut? I'm especially
interested in someone with good experience with the
Leica instruments.
Thank you

Marc
--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 1 09:03:25 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, everyone:

I have some questions regarding DNA stains:

What are the advantages of using DAPI instead of Hoescht? In which
conditions should we use one or the other?

What are the main differences between the 2 Hoescht dyes?

I read about a new DNA stain, DRAQ5, has anyone used it? Can it be applied
to study apoptosis?

Thank you in advance,

Vera Santos

Instituto de Tecnologia Biom�dica

Faculdade Medicina Dent�ria

Universidade de Lisboa

Portugal

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 1 12:07:41 2004

Contents Retrieved from Microscopy Listserver Archives
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Hi Peggy,

I use Microscopical Optical Consulting, out of Valley Cottage NY. You
can reach them at 845-268-6450.

Ann Lehman
Electron Microscopy Facility
Mailstop: LSC314
Trinity College
300 Summit Street
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman

-----Original Message-----
} From: Sherwood, Margaret [mailto:MSHERWOOD-at-PARTNERS.ORG]
Sent: Friday, October 01, 2004 9:47 AM
To: 'Marc Pypaert'; 'Microscopy-at-MSA.Microscopy.Com'

Marc, et al,

I am also interested in getting a repairperson for my Ultracut E. It
really
needs an overhaul.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

-----Original Message-----
} From: Marc Pypaert [mailto:marc.pypaert-at-yale.edu]
Sent: Thursday, September 30, 2004 6:58 PM
To: 'Microscopy-at-MSA.Microscopy.Com'

After years of trying desperately to get one of our microtomes
working, I'm thinking of changing the company that provides
service on our instruments (2 Reichert Ultracut E and 2 Leica
UCT/FCS). Does anyone in the NorthEast know of someone
capable of providing service in Connecticut? I'm especially
interested in someone with good experience with the
Leica instruments.
Thank you

Marc
--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 1 15:02:20 2004

Contents Retrieved from Microscopy Listserver Archives
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Hi:

We just installed a new TEM and it was suggested that we use a 'demand
regulator' to control the flow of nitrogen during specimen exchange and/or
venting the column etc. The installing engineer didn't have the details for
ordering etc.

Ok, so tell me about demand regulators and where to get one.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 1 18:15:35 2004

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Colleagues,

Our Mini-Workshop Series on "Cryoultramicrotomy for Materials Science" is
coming to Akron, Ohio!

**When**:
Thursday, October 14 - Friday, October 15, 2004, 9:00am-4:00pm

**Where**:
University of Akron
Department of Polymer Engineering
Polymer Engineering Academic Center (PEAC)
Room 307
250 South Forge Street
Akron, OH 44325

**What**:
A two-day mini-workshop with presentations, demonstrations and hands-on
sessions for cryoultramicrotomy, including toolmaking (wire loops and hair probes),
glass knife making, evaluation of glass knives, care and cleaning of diamond
knives and operation of the cryoultramicrotome.

Lectures, demonstrations and an introduction to the cryoultramicrotome will
be given on the first day; open lab sessions and hands-on use of the
instrumentation in small groups will be the focus of the second day.

Demonstrations will be given using well-characterized specimens which are
provided by the instructors. There is a limited amount of time to work with
specimens provided by attendees. If you would like to bring your own specimens,
please provide details on the materials when you RSVP and reserve a place in
the workshop.

**Background**:
These techniques are of special interest to anyone working with polymers or
other materials which could benefit from sectioning or surface "polishing"at
low temperatures (no embedding required). The sections may be used for TEM,
optical microscopy, IR spectroscopy and FTIR. The polished surface of the bulk
material is suitable for AFM, SEM, X-ray microanalysis and elemental mapping.

**Important Info**:

1. There is no charge for this workshop.

2. Meals and refreshments will be served!

3. Attendance is open to everyone for the presentations and demonstrations
on the first day. However, attendance is limited for the cryoultramicrotomy
hands-on sessions on the second day.

**RSVPs and Reservations**:
To RSVP and to reserve a spot for the hands-on sessions, please contact Kim
Megaw at Boeckeler Instruments, Inc. ( {kim-at-boeckeler.com} , 800.552.2262).

**Sponsors and Organizers**:
University of Akron
Department of Polymer Engineering
RMC Products Group, Boeckeler Instruments, Inc.

See you in Akron!

Bob Chiovetti
Senior Product Specialist
Boeckeler Instruments, Inc.

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 1 20:41:20 2004

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Jon,

Check your local scuba shop. I used some PVC (Tygon) tubing, a few hose
clamps, and a few copper plumbing fittings to adapt a scuba regulator for
my dry nitrogen backfill.

I hose-clamped a piece of large diameter Tygon over the mouthpiece then
downsized with the Cu fittings to a 1/4" hose barb which hooked to the hose
to the scope. On the supply side, I cut the original fitting off the high
pressure hose and used a brass hose barb to connect it to my nitrogen gas
regulator.

Have fun explaining to your financial people why you needed to buy a scuba
regulator!

Cheers,
Henk

At 04:03 PM 10/1/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
OSU Campus Electron Optics Facility www.ceof.ohio-state.edu
040 Fontana Labs, 116 W. 19th Ave

From MicroscopyL-request-at-ns.microscopy.com Sat Oct 2 03:31:27 2004

Contents Retrieved from Microscopy Listserver Archives
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Why is this stuff not being supplied by the vendor, I wonder.

Chris Jeffree
University of Edinburgh

----- Original Message -----
From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-microscopy.com}
Sent: Friday, October 01, 2004 9:03 PM
Subject: [Microscopy] Demand regulator

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi:
}
} We just installed a new TEM and it was suggested that we use a
} 'demand
} regulator' to control the flow of nitrogen during specimen exchange
} and/or
} venting the column etc. The installing engineer didn't have the
} details for
} ordering etc.
}
} Ok, so tell me about demand regulators and where to get one.
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Sun Oct 3 09:40:29 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ghina_24-at-yahoo.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, October 3, 2004 at 00:06:00
---------------------------------------------------------------------------

Email: ghina_24-at-yahoo.com
Name: ghina husnulnisa

Organization: university of andalas

Education: Undergraduate College

Location: padang,west sumatra,indonesia

Question: Do you know about composition of may-gruenwald's eosin methylene blue solution ?
thank's for your answer.

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Sun Oct 3 17:51:38 2004

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__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 07:04:34 2004

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Tina,
I recently fixed yeast cells for the first time and also found that it is hard to see the membranes. I followed a protocol suggested by Mark Winey which referenced a paper by Byers (Byers, B. and Goetsch, L. (1991) Preparation of yeast cells for thin section electron microscopy. Methods Enzymol. 194: 602-608.) This procedure involves spheroplasting which removes the cell wall, thus allowing good infiltration. The cells turned out looking great but the people I did the work for wanted to see more membranes.

Another good reference for fixing yeast is a paper by Robin Wright (Wright,R. (2000) Transmission Electron Microscopy of Yeast. Microsc. Res. Tech. 51:496-510). She gives alot of helpful hints for fixing yeast. She gives a detailed procedure in the paper using potassium permanganate as a post fixative. If you use potassium permanganate as a post fixative you don't have to spheroplast the cells (according to the paper, I have not tried it yet). Of course we all know that potassium permanganate extracts much of the cytoplasm, which is perfect for viewing membranes. I hope this will be helpful.

Janice Pennington
Senior Electron Microscopist
Nephrology/Anatomy and Cell Biology
Indiana University School of Medicine
635 Barnhill Drive, MS 5065
Indianapolis, IN 46202
Phone (317) 274-8730
Fax (317) 278-2040

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 09:10:21 2004

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Dear Listers,

I would like to open a discussion about the use of Biosafety Level 2
regulated cells, tissues and organisms in a multi-user microscopy
facility not designed to house BSL2 regulated agents (no once-through
air or biosafety cabinet).

Is it customary to have researchers use the microscopy facility if BSL2
regulated agents are alive but completely contained in unbreakable and
well-sealed containers? While such conditions are easily accomplished
for transport of materials, they are less so for the actual viewing.
How is this commonly resolved?

Is it customary for users to write a protocol for microscope use,
spelling out what to do in an emergency situation?

Is expected from microscopy facility personnel to know exactly what to
do in case of an emergency situation (e.g. if somebody works on BSL2
regulated bacteria in a flow cell and one of the connections breaks
during microscope operation, spilling the content over microscope
equipment), or is this the responsibility of the investigator?

In case of spillage, a broken slide, etc., how does one deal with the
resulting contaminated equipment?

Is it customary to have every user fill out and sign a form explaining
what organisms they plan to use, how it is regulated and how they plan
to use those organisms in the microscopy facility?

Thank you for your input.

All the best,
Judith.

Judith Wopereis
Microscopy Facility Manager and
Instructor of Laboratories in Biological Sciences
Smith College
Northampton, MA 01063
(413) 585 3829 (voice mail)
(413) 585 3786 (fax)
jwoperei-at-email.smith.edu

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 09:12:36 2004

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We purchased ours through our local compressed gas vendor.

Hendrik O. Colijn wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Jon,
}
} Check your local scuba shop. I used some PVC (Tygon) tubing, a few
} hose clamps, and a few copper plumbing fittings to adapt a scuba
} regulator for my dry nitrogen backfill.
}
} I hose-clamped a piece of large diameter Tygon over the mouthpiece
} then downsized with the Cu fittings to a 1/4" hose barb which hooked
} to the hose to the scope. On the supply side, I cut the original
} fitting off the high pressure hose and used a brass hose barb to
} connect it to my nitrogen gas regulator.
}
} Have fun explaining to your financial people why you needed to buy a
} scuba regulator!
}
} Cheers,
} Henk
}
}
} At 04:03 PM 10/1/2004, you wrote:
}
}
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} }
} } Hi:
} }
} } We just installed a new TEM and it was suggested that we use a 'demand
} } regulator' to control the flow of nitrogen during specimen exchange
} } and/or
} } venting the column etc. The installing engineer didn't have the
} } details for
} } ordering etc.
} }
} } Ok, so tell me about demand regulators and where to get one.
} }
} } Thanks
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
}
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} OSU Campus Electron Optics Facility www.ceof.ohio-state.edu
} 040 Fontana Labs, 116 W. 19th Ave
}
}
}

--
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 10:14:05 2004

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I would like to know if there is a mixture of epoxy and carbon powders for ion-
thinning in market. A researcher from Hungary made a low angle ion-thinner
(IV3?), and also he confirmed that if you use C powder with epoxy, you can ion-
thin fine grain materials like powder. I do not remember the reference now,
but you can get a lower thinning rate of the C and epoxy mixture, according to
the paper.
I used a kit for ion-thinning in Japan. Probably, it was imported from
Hungary.
If you use similar technique, please advise where I can get the powdered epoxy
and C.

Thank you,
Hiromi Konishi, Ph.D.
Indiana University

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 10:29:26 2004

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We have also used these on several instruments at our facility. Alex Greene
set them up. The only difference was he filled the mouthpiece with RTV
sealant and used a secondary output on the regulator to connect to the
scope. I am not sure if all SCUBA regulators have this output, or if the
ones he found were special.

One problem you might find is if the demand is too high or the supply too
low, the small silicone diaphragm used for exhaling can invert allowing room
air into the system. I have also always worried (however with no proof)
that the line between the regulator and the instrument, being at zero
pressure WRT the room, can become contaminated with room air.

On our latest TEM and SEM installs, we have not used these since the
manufactures have included doors or popup valves that open when the pressure
difference becomes zero WRT the room.

-Ray

Ray D. Twesten, PhD.
Center for Microanalysis of Materials
Seitz Materials Research Laboratory
104 S. Goodwin Ave., Urbana, IL 61801
+1 217 244-6177 (Fax -2278)

-----Original Message-----
} From: colijn-at-er6s1.ecr6.ohio-state.edu
[mailto:colijn-at-er6s1.ecr6.ohio-state.edu]On Behalf Of Hendrik O. Colijn
Sent: Friday, October 01, 2004 8:47 PM
To: Microscopy-at-microscopy.com

Jon,

Check your local scuba shop. I used some PVC (Tygon) tubing, a few hose
clamps, and a few copper plumbing fittings to adapt a scuba regulator for
my dry nitrogen backfill.

I hose-clamped a piece of large diameter Tygon over the mouthpiece then
downsized with the Cu fittings to a 1/4" hose barb which hooked to the hose
to the scope. On the supply side, I cut the original fitting off the high
pressure hose and used a brass hose barb to connect it to my nitrogen gas
regulator.

Have fun explaining to your financial people why you needed to buy a scuba
regulator!

Cheers,
Henk

At 04:03 PM 10/1/2004, you wrote:

} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
OSU Campus Electron Optics Facility www.ceof.ohio-state.edu
040 Fontana Labs, 116 W. 19th Ave

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 11:44:37 2004

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Hi Tina,

First of all, I can vouch for the Wright protocol for preparing yeast for
TEM mentioned by Janice (1. below). Works great! Membranes stand right out
there, resins infiltrate through wall completely into cell (I used
Embed812).

In your query (2. below) you mention that the ultimate goal of your study is
to look for copper in mitochondria in yeast, using EELS or whatever
analytical technique. For elemental analysis in sections, given all the prep
they usually go through, there is always the danger of leaching out the
element you are interested in looking for. Any elements/compounds that are
water soluble - they're gone! So even if you succeed in getting good
membrane and mitochondrial fixation, may not be any copper left. Do you know
for sure if the copper is bound in the mitochondria?

I have heard that freeze-substitution does a better job of preserving
elements in place for elemental analysis - you'd just have to try it and
see. Or try freeze-drying small masses of sample, then vacuum infiltrate
with resin.

Good luck! Gib
--
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-2785 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

"You can learn a lot by observation - just by lookin'!" - Yogi Berra
-------------

1. ---------
Tina,
I recently fixed yeast cells for the first time and also found that it is
hard to see the membranes. I followed a protocol suggested by Mark Winey
which referenced a paper by Byers (Byers, B. and Goetsch, L. (1991)
Preparation of yeast cells for thin section electron microscopy. Methods
Enzymol. 194: 602-608.) This procedure involves spheroplasting which
removes the cell wall, thus allowing good infiltration. The cells turned
out looking great but the people I did the work for wanted to see more
membranes.

Another good reference for fixing yeast is a paper by Robin Wright
(Wright,R. (2000) Transmission Electron Microscopy of Yeast. Microsc. Res.
Tech. 51:496-510). She gives alot of helpful hints for fixing yeast. She
gives a detailed procedure in the paper using potassium permanganate as a
post fixative. If you use potassium permanganate as a post fixative you
don't have to spheroplast the cells (according to the paper, I have not
tried it yet). Of course we all know that potassium permanganate extracts
much of the cytoplasm, which is perfect for viewing membranes. I hope this
will be helpful.

Janice Pennington
Senior Electron Microscopist
Nephrology/Anatomy and Cell Biology
Indiana University School of Medicine
635 Barnhill Drive, MS 5065
Indianapolis, IN 46202
Phone (317) 274-8730
Fax (317) 278-2040

2. -----------------
Hello, All-

Last year a couple of students from BYU Hawaii were interested in looking
for copper in the mitochondria of yeast by EELS and/or ESI, using our LEO
912 EFTEM. The first (but by no means the only) stumbling block was
getting decent ultrastructure, or at least good enough that they could
identify the mitochondria, and good enough infiltration that 25-30 nm
sections could be obtained.

I do not have any experience with yeast and so I am open to any and all
hints, tips, and suggestions!

If this works out, I invite you all to come to see the results at M&M 2005
in Honolulu next August.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 12:28:39 2004

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Dear Hiromi:

The Low Angle Ion Thinner you are referring to is the IV3 from
Technoorg-Linda in Budapest. Actually, they make the IV3 with high
energy guns, low energy guns as well as a focussed ion gun. They also
produce the Gentle Mill which is a dedicated low energy ion polisher.

As the distributor for Technoorg-Linda products in North America, we do
offer the carbon/epoxy product that you reference. I will contact you
offline with the part number and pricing.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David

hkonishi-at-indiana.edu wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 13:25:49 2004

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Hi Tina,
Yeast cells freeze quite nicely. I think the closest high pressure
freezer to you is located in Kent McDonald's lab at Berkeley .

Beth

On Thursday, September 30, 2004, at 05:53 PM, Tina Carvalho wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Hello, All-
}
} Last year a couple of students from BYU Hawaii were interested in
} looking
} for copper in the mitochondria of yeast by EELS and/or ESI, using our
} LEO
} 912 EFTEM. The first (but by no means the only) stumbling block was
} getting decent ultrastructure, or at least good enough that they could
} identify the mitochondria, and good enough infiltration that 25-30 nm
} sections could be obtained.
}
} I do not have any experience with yeast and so I am open to any and all
} hints, tips, and suggestions!
}
} If this works out, I invite you all to come to see the results at M&M
} 2005
} in Honolulu next August.
}
} Aloha,
} Tina
}
} ***********************************************************************
} *****
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
} *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} ***********************************************************************
} *****
}
}
}
**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 16:03:18 2004

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But how does one know which marker pens are OK and which are not?

cheers

rtch

}
} One thing of note....
}
} DO NOT use sharpie markers or any other acid base pen to label cd's or
} dvd's. The acid in these pens etches through the top of the disk
} destroying your data.
}
} Dave Crone B.E. (Mechanical)
} Engineer-in-Training
} Department Assistant
} Metallurgical Lab
} Mechanical Engineering
} College of Engineering
} University of Saskatchewan
} 57 Campus Drive
} Saskatoon, SK
} S7N 5A9
} Phone: (306) 966-5461
} Fax: (306) 966-5427

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 18:24:47 2004

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It is a safe bet that if they are sold on the premise that they are cd safe
they should be good

Dave Crone B.E. (Mechanical)
Engineer-in-Training
Department Assistant
Metallurgical Lab
Mechanical Engineering
College of Engineering
University of Saskatchewan
57 Campus Drive
Saskatoon, SK
S7N 5A9
Phone: (306) 966-5461
Fax: (306) 966-5427
E-mail: dgc132-at-mail.usask.ca
dgc132-at-gmail.com
-----Original Message-----
} From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz]
Sent: Monday, October 04, 2004 3:06 PM
To: Dave Crone; 'MICROSCOPY'

But how does one know which marker pens are OK and which are not?

cheers

rtch

}
} One thing of note....
}
} DO NOT use sharpie markers or any other acid base pen to label cd's or
} dvd's. The acid in these pens etches through the top of the disk
} destroying your data.
}
} Dave Crone B.E. (Mechanical)
} Engineer-in-Training
} Department Assistant
} Metallurgical Lab
} Mechanical Engineering
} College of Engineering
} University of Saskatchewan
} 57 Campus Drive
} Saskatoon, SK
} S7N 5A9
} Phone: (306) 966-5461
} Fax: (306) 966-5427

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 19:10:31 2004

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I have used Sharpies many times to mark disks, and have never seen a
problem yet. I should add that I always use top-coated disks.

John Mardinly
Intel

-----Original Message-----
} From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz]
Sent: Monday, October 04, 2004 2:06 PM
To: Dave Crone; 'MICROSCOPY'

But how does one know which marker pens are OK and which are not?

cheers

rtch

}
} One thing of note....
}
} DO NOT use sharpie markers or any other acid base pen to label cd's or
} dvd's. The acid in these pens etches through the top of the disk
} destroying your data.
}
} Dave Crone B.E. (Mechanical)
} Engineer-in-Training
} Department Assistant
} Metallurgical Lab
} Mechanical Engineering
} College of Engineering
} University of Saskatchewan
} 57 Campus Drive
} Saskatoon, SK
} S7N 5A9
} Phone: (306) 966-5461
} Fax: (306) 966-5427

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9
3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 19:36:57 2004

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Hi,

I agree with Henk and Mike. I believe CDRs ARE archival grade if handled
properly. Even those thousand year old clay tablets, suggested in jest,
are almost useless if broken or turned to dust from improper handling.

I did not read the whole article but I what to address the Sharpie� acidic
ink problem. In the interest of having all this CDR stuff in one posting,
I offer this web information:

I used Sharpie pens for years on CDRs without a problem.
The acidic pen posting raised these questions:
Were my Sharpie� pens the acidic types?
Was my first official US flag research CD going to disintegrate?
So, I looked to SandfordCorp.com to see what they said about acidic pens.

http://www.sanfordcorp.com/sanford/consumer/jhtml/help/sanford_help_922.jhtml

Quoting:
"There are two criteria, which identify "Acid Free" Products:
1. The product must contain no added acid
2. The product must contain an ink with a pH specification of 7 or above,
or the product has no measurable pH because it contains a solvent - based
ink or it is a solid. (The pH cannot be measured if the ink does not
contain water)

Product Color
(snip other types of markers that are not permanent)

Permanent Markers
7000 Marker All Colors
7007 Marker All Colors
Deluxe Marker - All Colors Not Black
King Size Marker - All Colors Not Black
Liquid Tip Marker All Colors
Magnum 44 - All Colors Not Black
Mean Streak All Colors
Rub-A-Dub Black
Sharpie Autograph Black
Label Pen Black
Penguin Freezer Wrap Marker Black
T.E.C. Markers Black (Trace Element free pens)
Sharpie Industrial Black "
End quote.

It should be obvious what to use.
I used black Industrial and Label pens without any problems, IMO.

Disclaimer: I don't work for Sanford Corp but I like Sharpie� pens.

My thanks to Hendrik O. Colijn at The Ohio State University for providing
the original reference.

Paul Beauregard
Senior Research Associate

-----------------------------------------------------------------------
} Original Sharpie Pen post:
} One thing of note....
} DO NOT use sharpie markers or any other acid base pen to label cd's or
} dvd's. The acid in these pens etches through the top of the disk destroying
} your data.
}
} Dave Crone B.E. (Mechanical)
}
End Beauregard postings.
-------------------------------------------
At 10:28 AM 9/27/04 -0700, Michael O'Keefe wrote:
}
} Henk,
}
} That's an extremely informative and useful article. Thank you!
}
} It was interesting to learn that CD RW and DVD RW (bits encoded in metal
structure
} by a phase change from crystalline to amorphous) are less stable over time
than CD
} R and DVD R (bits encoded in degradable organic dyes). And that RW discs
that are
} "cycled" a lot (many re-writes) will degrade faster.
}
} The best advice seems to be to use R (write once) discs (CD or DVD) and
store them
} vertically in a cool, low-light environment. Of course, transferring to
fresh
} media every year or so couldn't hurt...
}
} Mike
}
} "Hendrik O. Colijn" wrote:
}
} }
------------------------------------------------------------------------------
} }
} } Hi all,
} }
} } I just ran across this reference in our local newspaper about the care and
} } storage of CDs and DVDs. It is copublished by CLIR (Council on Library and
} } Information Resources) and NIST, so it should be reliable info.
} }
} } http://www.clir.org/pubs/reports/pub121/contents.html
} }
} } From my quick run through of the article, it appears that DVDs may be
} } inherently more stable than CDs.
} }
} } Cheers,
} } Henk
} }
} } Hendrik O. Colijn colijn.1-at-osu.edu
} } Campus Electron Optics Facility Ohio State University
} } (614) 292-0674 http://www.ceof.ohio-state.edu
} } Time is that quality of nature which keeps events from happening all at
} } once. Lately it doesn't seem to be working.

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 19:58:26 2004

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Ritchie,

According to the article I read, the issue seems to be damage to the
lacquer on the top surface of the CD. The author of the article recommends
water based markers or perhaps alcohol based, but not other solvent
based. For example, I've got a marker which appears to be xylene-based
(sniff test!) and probably damaging to the protective lacquer. I certainly
found it interesting that the top-surface is more delicate than the bottom!

For details, see below...
http://www.clir.org/pubs/reports/pub121/contents.html

Cheers,
Henk

At 05:06 PM 10/4/2004, Ritchie Sims wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
OSU Campus Electron Optics Facility www.ceof.ohio-state.edu
040 Fontana Labs, 116 W. 19th Ave

From MicroscopyL-request-at-ns.microscopy.com Mon Oct 4 21:07:25 2004

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Greetings,

Optical Elements Corporation (OPELCO) has current openings for an Image
Analysis Sales Specialist and a Confocal Microscopy Sales Specialist in
the Mid-Atlantic region. If interested, please click on the following
link or contact me directly.

http://www.opelco.com/employmentcontact.htm

Best Regards,

Cliff Glier
COO
OPELCO
105 Executive Drive Suite 100
Dulles, VA 20166
703.471.0080 x230
703.904.9432 (fax)
cglier-at-opelco.com
www.opelco.com

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 10:29:47 2004

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Paul,

Thanks for your info. I went to the Sanford site and noticed that they
provide MSDS sheets for their markers. Interestingly, Sharpies do not all
use the same solvents. I'm not an organic chemist, so I would appreciate
if someone would correct me... If I remember correctly, n-propanol and
n-butanol are alcohols and should not affect the lacquer. I don't know
about diacetone alcohol or about the ethers. Based on my uneducated guess,
I would probably avoid the naptha solvents in the "Sharpie Professional"
series.

Can anyone provide additional info about lacquers and solvents?

Sharpie Fine Point Permanent, Sharpie Twin-tip, Super Sharpie, Super
Sharpie Twin-tip
n-propanol, n-butanol, diacetone alcohol

Sharpie Ultra Fine Point
n-propanol, n-butanol, diacetone alcohol, isopropyl alcohol, ethyl alcohol

Sharpie Industrial Fine Point
ethylene glycol monobutyl ether, ethylene glycol monoethyl ether, ethyl alcohol

Sharpie Professional
nitroparaffin solvent, solvent naptha

Cheers,
Henk Colijn

At 08:41 PM 10/04/04, Beauregard wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all at
once. Lately it doesn't seem to be working.

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 12:41:49 2004

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Dear All,

I've recently started taking birefringence photographs of Congo Red stained
tissue. I've noticed that over time, the very same field doesn't appear the
same, and that the labeled surface varies up to 25%.

Everything being very stable (all screws firmly screwed, polarizer glued to
its base), I'm wondering if anyone has performed a test to check the warm-up
period of a halogen 6 volt lamp. The company who sells the lamp says 5 to 10
minutes, but could it be longer?

Thank you!

Marie-Claude Belanger

_________________________________________________________________
Des m�canismes de contr�le parental puissants permettent � votre enfant de
d�couvrir tout ce qu�Internet a � offrir.
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From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 12:57:22 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listserver,

Does anyone have a good reciepe for TEM
fixation of ebryonic cell cultures? (ie 293
kidney). Are these cell's osmolarities generally
higher or lower than other cell types. Thanks.

Mike Delannoy

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 16:38:06 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (monica.iliescu-at-polymtl.ca) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, October 5, 2004 at 10:47:16
---------------------------------------------------------------------------

Email: monica.iliescu-at-polymtl.ca
Name: Monica ILIESCU

Organization: Ecole Polytechnique

Title-Subject: [Microscopy] [Filtered] Sample preparation

Question:
Does some know how prepare the biological samples for ESEM observations? We have an Quanta FEG 200, FEI Company, and we want to prepare for observation a sample from chitosan solution, to see the chitosan nanoparticles.
Thank you!

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 16:38:57 2004

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (aiqbal-at-email.arizona.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, October 5, 2004 at 13:21:33
---------------------------------------------------------------------------

Email: aiqbal-at-email.arizona.edu
Name: Asad Iqbal

Organization: University of Arizona

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Please if some one could help me with this.
I would like to know if there is any software available to measure thickness and composition of the sample using SEM/EDS. Where can I get it from?

Thank You,

Regards,
Asad

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 16:43:13 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for help imaging active drug particles in an aqueous pharmaceutical suspension. I am trying to locate someone who can view frozen sections of this material in the 100 angstrom size range. I believe this will require an environmental SEM with a cryogenic stage. I would appreciate any help. Thank you.

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 17:05:09 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
I have not used NIH Image or Scion Image and a student in my department
has the following question:

} I'm looking at leaf morphology in a number of tree species, and I've
} got 100's of flatbed leaf scans that I'm trying to analyze using Scion
} Image (a PC spin-off of NIH Image) and ImageJ. The problem I'm having
} is that the program is recognizing (and measuring) the background as
} well as the leaf in its area calculations. Do you have any advice or
} could you help in any way in dealing with this problem?

Any help would be greatly appreciated!
thanks in advance,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 17:19:46 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
} I believe CDRs ARE archival grade if handled
} properly.

That (handled properly) is indeed the problem with this class of media.

As was mentioned in the report referenced by an earlier post, (if you
read between the lines), this media CAN be very easily damaged.

As an IT Manager, I would suggest you think about what you mean by
"Archive" class media.

If you mean something that you can hide away in a controlled
environment, and carefully access at some point in the future, then
maybe this class of media is appropriate.

If you mean something that you can use for routine "archive" access (as
opposed to "on-line" access) I would strongly suggest that the media CAN
be too sensitive to physical damage (more so on the label side than the
"bottom" side).

If you mean something that will absolutely, positively be there when you
need it for "disaster recovery", I would suggest that this isn't the
media you want for that.

I don't mean to say the media isn't durable, or won't last. Just that
there is a chance that a single disk may be easily damaged, either by
natural causes (thermal cycling, humidity, etc.) or accidentally by
routine handling. My friend Murphy says that this will happen on the
most important area of the most important disk at the most inopportune
time.

I have lived the nightmare of backup systems not working for a number of
reasons. You don't want to go there.

John W. Raffensperger, Jr.
IT Manager
Apache Stainless Equipment

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 17:35:18 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to know the best way to prepare ion-thinning samples of small
particles. The size is several 10 um. I know that FIB or microtome might work
better, but I want to try ion thinning. The material is mineral like SiO2.

If you embed small grains in epoxy, the grains drop during milling in many
cases because thinning rates of sample and epoxy are different. Also, rough
surface that are produced by preferential etching is another problem. It is
hard to make good ion-thinning sample of small grains in general.

Using Araldite (AT1) containing C or M-bond 610 can reduces the problem of
preferential etching of glue (Barna 1992, Westman et at 1999, McCaffrey and
Barna 1997). (SBT sells "Carbon powder & Araldite-Type AT1" for low angle ion
milling). Photo-resist is "hard" in ion milling (Yoshioka at al 1995).

I do not have any other idea that may reduce the problem in preferential
etching. If you have any suggetions, please advise. Also, if someone used AT1
with Gatan Dual Mill or PIPS, I would like to know if you got better result or
not.

Thank you,

Hiromi Konishi, Ph.D.
Indiana University
www.unm.edu/~hkonishi

From MicroscopyL-request-at-ns.microscopy.com Tue Oct 5 19:22:37 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It is probably trivial but ...

.. does the student threshold and binarize the picture before to do
the analysis ?

1) Threshold and choose the level he wants

2) Binarize (black & white picture, 0 or 256)

3) Analyse (and if the program take everything & not only the
particles, try invert picture. I don't remember if NIH or Scion Image
consider the white or the black spots as particles...).

Hope it helps

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Christian Wuethrich, PhD
Harvard Medical School
Division of Viral Pathogenesis
Beth Israel Deaconess Medical Center
Research East. Room 217
41 Av. Louis Pasteur
Boston, MA 02115
United States

Phone: 001-617-667-2143
Fax: 001-617-667-8210

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 00:37:19 2004

Contents Retrieved from Microscopy Listserver Archives
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Friends
on Darwin, a new italian journal on science and science policy, there
is an article on Multiphoton microscopy "Il radioso futuro della
microscopia ottica" and also the cover has been dedicated to
multiphoton microscopy. I'd like to thank Kenneth Dunn for providing
one of the images of the article collected and rendered by Ruben
Sandoval at the Indiana Center for Biological Microscopy. Website is
www.darwinweb.it.
All my best
ALby

------------------------------------------------------------------------
---------------------------
Alberto Diaspro, Department of Physics, University of Genoa
Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309
URL: http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html
------------------------------------------------------------------------
--------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 03:05:53 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Asad,

layer thickness measurement within the Electron Microscope and with the
EDX-spectrometer with electron excitation is possible, but is faced to a
lot of limits. The penetration-range of electrons in specimen is
strongly limited. If the layer is going to become thicker (depends from
primary electron energy, typically 1 micron or less), saturation is
reached, no reliable results are possible. The calculation of all
interactions are complex. If there are multi-layers, the electrons have
to travel through the top layers... Therefore layer-measurements are
only possible with very thin layers.

With an X-ray excitation the entire situation is more friendly and
therefore more reliable. Thicknesses of 1 nm up to 50 microns are
possible to deal with. Because of the larger range of X-ray penetration
in specimen, multi-layers are not a problem. An X-ray excitation in SEM
is available now and proper software for calculation thickness and
concentrations with X-ray excitation, as well: see:
http://www.ifg-adlershof.de/sem.htm

Best regards

Frank Eggert

====================
http://www.microanalyst.net
====================

by way of MicroscopyListserver wrote:

------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (aiqbal-at-email.arizona.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, October 5, 2004 at 13:21:33
---------------------------------------------------------------------------

Email: aiqbal-at-email.arizona.edu
Name: Asad Iqbal

Organization: University of Arizona

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Please if some one could help me with this.
I would like to know if there is any software available to measure thickness and composition of the sample using SEM/EDS. Where can I get it from?

Thank You,

Regards,
Asad

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 07:31:41 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,
Then what would a "good" method of data archive? Hardware RAID and tape?

---
Robb Westby
Senior SEM/FIB Technician
Maxim Integrated Products

On Tue, 2004-10-05 at 15:23, Chiphead wrote:
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} }
} } I believe CDRs ARE archival grade if handled
} } properly.
}
} That (handled properly) is indeed the problem with this class of media.
}
} As was mentioned in the report referenced by an earlier post, (if you
} read between the lines), this media CAN be very easily damaged.
}
} As an IT Manager, I would suggest you think about what you mean by
} "Archive" class media.
}
} If you mean something that you can hide away in a controlled
} environment, and carefully access at some point in the future, then
} maybe this class of media is appropriate.
}
} If you mean something that you can use for routine "archive" access (as
} opposed to "on-line" access) I would strongly suggest that the media CAN
} be too sensitive to physical damage (more so on the label side than the
} "bottom" side).
}
} If you mean something that will absolutely, positively be there when you
} need it for "disaster recovery", I would suggest that this isn't the
} media you want for that.
}
} I don't mean to say the media isn't durable, or won't last. Just that
} there is a chance that a single disk may be easily damaged, either by
} natural causes (thermal cycling, humidity, etc.) or accidentally by
} routine handling. My friend Murphy says that this will happen on the
} most important area of the most important disk at the most inopportune
} time.
}
} I have lived the nightmare of backup systems not working for a number of
} reasons. You don't want to go there.
}
} John W. Raffensperger, Jr.
} IT Manager
} Apache Stainless Equipment
}
}
}
}

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 08:43:55 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Already a couple of years ago, former colleagues did automated
calcium-ratio measurements with CCD-camera's on individual
(cardio)myocytes in cell cultures. They used a modified CCD-camera which
allowed them to capture an image of an individual (cardio)myocyte every
10 milliseconds to monitor intracellular calcium.

I am wondering which type of camera could now be used to give the same
time-resolution (10 msec. frame time) for individual cells (the hardware
they used is now no longer available) ?

Reference:

Cornelissen, F., Wouters, L., Ver Donck, L., Verellen, G., Geerts, H.
Automatic quantification of the effect of cardioprotective drugs in
isolated myocytes.
Bioimaging, 1993, 1, pp. 197-206.

Regards,

Peter Van Osta

----------------------------------------------
Peter Van Osta

Director Imaging
MAIA SCIENTIFIC
Cipalstraat 3
B-2440 Geel, Belgium
Tel.: +32 (0)14 570 620
Mobile: +32 (0)497 228 725
Fax.: +32 (0)14 570 621
Email: pvosta-at-maia-scientific.com
Website: www.maia-scientific.com
A Harvard Bioscience Company
----------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 10:08:41 2004

Contents Retrieved from Microscopy Listserver Archives
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In my opinion (for what it may be worth) tape has
proven to be, and remains, the best "archival" media.
It has proven to be the most durable, and most likely
to be available when needed.

Raid would come into play not so much for archive, but
for "availability". If you can live with the data
being unavailable for a period of time while a new
disk drive is put in place (if required) and the
backup restored, then raid is not necessarily
required. If you NEED to have the data available NOW,
then raid provides some fault tolerance, and the
ability to keep the data available while the recovery
is in process.

Tape is not perfect, it can be destroyed, but it is
less likely to have the types of single use failure
that CD/DVD media are susceptible to. They can take
an awful lot of environmental and physical abuse, and
still be readable.

Don't get me wrong, CD/DVD have their place. I use
them all the time. Just not for archive, and I never
use them to hold the only copy of something (even on
multiple disks) that needs to be kept. For cases
where the data could not be kept on the hard drives, I
would make a tape backup, then a set of CD/DVDs for
the users.

John Raffensperger

--- Robb Westby {robbw-at-mxim.com} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} John,
} Then what would a "good" method of data archive?
} Hardware RAID and tape?
}
} ---
} Robb Westby
} Senior SEM/FIB Technician
} Maxim Integrated Products
}
}

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 15:14:26 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Would using hepes as a buffer for reducing glutaraldehyde
with Nh4Cl be a problem? I know hepes isn't compatible with
osmium, but what about other IEM chemicals say tannic acid?
thanks
Mike D.

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 17:35:15 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We use HEPES for paraformaldehyde/glutaraldehyde in the primary fix, with
50 mM glycine in the blocking step, and with 1% osmium. why do you think
it is incompatible with osmium?

At 03:18 PM 10/06/04, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 18:17:59 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bwareham-at-utah.gov) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, October 6, 2004 at 16:06:52
---------------------------------------------------------------------------

Email: bwareham-at-utah.gov
Name: Beverly Wareham

Organization: Utah Veterinary Diagnostic Laboratory

Title-Subject: [Microscopy] [Filtered] hydrogels/organogels

Question: Hi all,
I'm looking for a protocol to prepare some gel samples for SEM. They are referred to in papers as hydrogels or organogels. Any help with this is greatly appreciated.

Thanks,
Beverly Wareham
Utah Veterinary Diagnostic Laboratory
Logan, Utah

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 18:18:51 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (njt3-at-u.washington.edu) from http://msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, October 6, 2004 at 15:14:41
---------------------------------------------------------------------------

Email: njt3-at-u.washington.edu
Name: Jeanie Taylor

Organization: Univ of Wash

Education: Graduate College

Location: Seattle, Wa

Question: I am trying to calibrate the scale on an Olympus BH-2 compound scope. How do I check the measurement of the sacle in the eyepiece?

Thanks!

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 18:19:13 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mahtab977-at-yahoo.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, October 6, 2004 at 16:48:16
---------------------------------------------------------------------------

Email: mahtab977-at-yahoo.com
Name: Mahtab Shahkarami

Organization: San Jose State University

Education: Graduate College

Location: San Jose, CA USA

Question: I was wondering if anyone has had any experience using Si-chips (from Ted Pella) for growing cells. My purpose is to visualize attachment appendages of gram-negative bacteria, so a smooth surface is key. I'm not sure how much better Si-chips are than glass coverslips, and I also don't know how how to sterilize them (autoclave or ethanol?).

Furthermore, I want to thank you all for your responses to a past question of mine about HMDS drying- the procedure/tips I recieved were very useful.

-Mahtab Shahkarami

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 20:13:40 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Oct 6, 2004, at 4:22 PM, by way of MicroscopyListserver wrote:

} I'm looking for a protocol to prepare some gel samples for SEM. They
} are referred to in papers as hydrogels or organogels. Any help with
} this is greatly appreciated.
}
Dear Beverly,
Robert Apkarian has worked with these substances a lot, so check his
recent papers (if you have not already done so).
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 21:14:30 2004

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Another media which may be considered "archival quality" is magneto-optical
(MO) disks. They are mounted in the case and have a shelf life expectation
of 100+ years. As far as I know most of the government agencies used this
media to store our records at FBI and INS. MOs may be destroyed by
physical force (hammer?) or extreme temperature (fire). Tapes are great
too and more practical than MOs. The greatest disadvantage of MOs is that
they are slow and capacity is not very impressive (a couple of Gigs, which
is nothing nowadays). Modern CD/DVDs may not be considered as a good
storage solution. Meantime, we are using them in everyday life (with some
risk). The best known to me storage media is ceramic tablets. Sergey

At 08:12 AM 10/6/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From MicroscopyL-request-at-ns.microscopy.com Wed Oct 6 21:52:34 2004

Contents Retrieved from Microscopy Listserver Archives
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Pardon this commercial announcement.

Bioptechs does not make a habit of this type of announcement in this forum.
However, in this case I, think it is appropriate.

For the past 12 years we have been asked , by many users of this
list, for a micro-environmental cell culture chamber system for
upright microscopes. I am pleased to announce that we have finally
completed it! It is called the FCS3. It is based on the popular FCS2
technology. This system will enable any upright microscope equipped
with a digital camera to acquire long term ,live-cell , time-lapse
images of living organisms including mammalian specimens. Now, those
of you without an inverted microscope can easily accommodate
live-cell experiments with the same ease as inverted scope users.
Ideal for multi user facilities! Additional information is
available at: http://www.bioptechs.com/Products/FCS3/fcs3.html (This
link will take you directly to the appropriate page)

If you are offended by this posting please respond directly to me
with your admonishment.

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 00:30:19 2004

Contents Retrieved from Microscopy Listserver Archives
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I have been following the tread since we also store data. (as do all EM units!) I did not see the mentioning of hardisks as a option. I have thrown away (beginning of last year) my vintage 20meg hard disk that was purchased in 1989. My Pentium 1 was scrapped. It was still working perfectly with all the data intact. Hot-swapping allows fast and reliable backup easy. The cost of HD has dropped dramatically. Re installing is fast (hot swapping)
Just a thought.

-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Thursday, October 07, 2004 4:19 AM
To: Microscopy-at-microscopy.com

Another media which may be considered "archival quality" is magneto-optical
(MO) disks. They are mounted in the case and have a shelf life expectation
of 100+ years. As far as I know most of the government agencies used this
media to store our records at FBI and INS. MOs may be destroyed by
physical force (hammer?) or extreme temperature (fire). Tapes are great
too and more practical than MOs. The greatest disadvantage of MOs is that
they are slow and capacity is not very impressive (a couple of Gigs, which
is nothing nowadays). Modern CD/DVDs may not be considered as a good
storage solution. Meantime, we are using them in everyday life (with some
risk). The best known to me storage media is ceramic tablets. Sergey

At 08:12 AM 10/6/2004, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 06:22:49 2004

Contents Retrieved from Microscopy Listserver Archives
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We had a quick look at hydogels by putting them wet in an
ESEM.

Dave

On Wed, 06 Oct 2004 18:22:35 -0500 by way of
MicroscopyListserver {bwareham-at-utah.gov} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bwareham-at-utah.gov) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, October 6, 2004 at 16:06:52
} ---------------------------------------------------------------------------
}
} Email: bwareham-at-utah.gov
} Name: Beverly Wareham
}
} Organization: Utah Veterinary Diagnostic Laboratory
}
} Title-Subject: [Microscopy] [Filtered] hydrogels/organogels
}
} Question: Hi all,
} I'm looking for a protocol to prepare some gel samples for SEM. They are referred to in papers as hydrogels or organogels. Any help with this is greatly appreciated.
}
} Thanks,
} Beverly Wareham
} Utah Veterinary Diagnostic Laboratory
} Logan, Utah
}
} ---------------------------------------------------------------------------
}
}
}
} This incoming email to UWE has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"

This email has been independently scanned for viruses and any virus detected has been removed using McAfee anti-virus software

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 08:00:02 2004

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Mahtab Shahkarami wrote:
============================================================================
=
Question: I was wondering if anyone has had any experience using Si-chips
(from Ted Pella) for growing cells. My purpose is to visualize attachment
appendages of gram-negative bacteria, so a smooth surface is key. I'm not
sure how much better Si-chips are than glass coverslips, and I also don't
know how how to sterilize them (autoclave or ethanol?).
============================================================================
I have been lead to believe that at least some are using our own gold coated
Si-Chips for the growing of cells, see URL
http://www.2spi.com/catalog/submat/gold-coated-silicon-chips.shtml

The description of the uncoated silicon chips, also used for cell growth, is
given on URL
http://www.2spi.com/catalog/standards/silicon-chip-sample.shtml
The smoothness of the silicon surface is comparable to that of a glass cover
slip. The silicon used for their production is standard electronics
industry grade silicon wafers.

The uncoated silicon chips can certainly be autoclaved, however, we don't
have information on the survivability of the gold coated substrates under
autoclave conditions. We would appreciate knowing if anyone has such
information since we are asked that question from time to time.

Disclaimer: SPI Supplies manufactures both uncoated and gold coated silicon
chip substrates for cell growth applications.

Chuck
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 08:51:01 2004

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Hi,

My company is considering obtaining an Hitachi 806 cold field emitter
SEM. The organization that if offering it has not been pleased with its
performance, but is offering a really good deal. I was wondering if
anyone has experience with this tool? If so I would appreciate your
comments.

Thanks,

Gerhard
--
Gerhard S. Schoenthal, PhD
Director of Laboratories
Virginia Diodes, Inc.
321 West Main Street
Charlottesville, VA 22903

W: 434.297.3257
M: 434.409.7760
F: 530.884.5710

W: Schoenthal-at-vadiodes.com

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 08:59:21 2004

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Tom,
I once read on this listserver that organic
buffers shouldn't be used with osmium because they
could react (oxidized?). Although I will admit that
I have used reduced osmium with hepes with no noticeable artifacts (maybe due to the lower oxidation
state). I want to know if Hepes can be used as the
only buffer in a post-embed IEM protocol up to
uranyl acetate.

thanks
Mike Delannoy

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 09:39:09 2004

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Charles;

To answer your question about gold surviving autoclave on Si substrate,
you must state what the base metal is, e.g. Ti, Ti-Pt? I don't think you
put Au directly on the wafer without a base metal.

Peter Tomic
Agere Systems
Allentown, PA

-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: Thursday, October 07, 2004 10:04 AM
To: MICROSCOPY BB

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Mahtab Shahkarami wrote:
========================================================================
====
=
Question: I was wondering if anyone has had any experience using
Si-chips
(from Ted Pella) for growing cells. My purpose is to visualize
attachment appendages of gram-negative bacteria, so a smooth surface is
key. I'm not sure how much better Si-chips are than glass coverslips,
and I also don't know how how to sterilize them (autoclave or ethanol?).

========================================================================
====
I have been lead to believe that at least some are using our own gold
coated Si-Chips for the growing of cells, see URL
http://www.2spi.com/catalog/submat/gold-coated-silicon-chips.shtml

The description of the uncoated silicon chips, also used for cell
growth, is given on URL
http://www.2spi.com/catalog/standards/silicon-chip-sample.shtml
The smoothness of the silicon surface is comparable to that of a glass
cover
slip. The silicon used for their production is standard electronics
industry grade silicon wafers.

The uncoated silicon chips can certainly be autoclaved, however, we
don't have information on the survivability of the gold coated
substrates under
autoclave conditions. We would appreciate knowing if anyone has such
information since we are asked that question from time to time.

Disclaimer: SPI Supplies manufactures both uncoated and gold coated
silicon chip substrates for cell growth applications.

Chuck
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 09:44:27 2004

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (schoenthal-at-vadiodes.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, October 7, 2004 at 09:03:40
---------------------------------------------------------------------------

Email: schoenthal-at-vadiodes.com
Name: Gerhard Schoenthal

Organization: Virginia Diodes, Inc.

Title-Subject: [Microscopy] [Filtered] Opinions on Hitachi 806 SEM

Question: Hi,

My company is considering obtaining an Hitachi 806 cold field emitter SEM. The organization that if offering it has not been pleased with its performance, but is offering a really good deal. I was wondering if anyone has experience with this tool? If so I would appreciate your comments.

Thanks,

Gerhard
--
Gerhard S. Schoenthal, PhD
Director of Laboratories
Virginia Diodes, Inc.
321 West Main Street
Charlottesville, VA 22903

W: 434.297.3257
M: 434.409.7760
F: 530.884.5710

W: Schoenthal-at-vadiodes.com

---------------------------------------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 09:55:13 2004

Contents Retrieved from Microscopy Listserver Archives
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I have seen HEPES react with KMnO4 (it literally turns the mix into a gel
that then breaks down into time into a precipitate). Never had a problem
with either 1% osmium or 1% reduced osmium. After osmium, we usually rinse
with dH2O before ethanol dehydration since by this time the tissue is no
longer osmotically sensitive. We have done uranyl acetate in ethanol, or
sodium acetate buffer, or water at this stage. I haven't seen a lot of
difference but I think others are strong advocates of uranyl in the ethanol
or sodium acetate. If by post-embedding IEM you mean doing immunostaining
of osmicated, embedded tissues, you have a low probability of success with
most antigens in my experience. I have had a few antigens survive
osmication but generally only ones in high abundance to start. Most immuno
EM work using staining of sections would use aldehyde fixation
(formaldehyde only, or with low glutaraldehyde ~0.2% or sometimes a
standard 2% PF + 2.5% glut) followed by buffer rinses and then maybe a
uranyl acetate en bloc staining and dehydration before embedding in an
immuno friendly plastic like K4M, HM20, LRW, or LRG. LR Gold is my current
favorite but we find the choice is dependent on the antigen and we need to
re-invent the wheel each time and try them all. During our immunostaining
of sections, we use a HEPES buffer (70 mM NaCl, 30 mM HEPES, 2 mM CaCl2, pH
7.4) for the antibodies (usually with 0.1% BSA). good luck.

At 09:12 AM 10/07/04, you wrote:
} Tom,
} I once read on this listserver that organic
} buffers shouldn't be used with osmium because they
} could react (oxidized?). Although I will admit that
} I have used reduced osmium with hepes with no noticeable artifacts (maybe
} due to the lower oxidation
} state). I want to know if Hepes can be used as the
} only buffer in a post-embed IEM protocol up to
} uranyl acetate.
}
} thanks
} Mike Delannoy

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 09:58:22 2004

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} Date: Thu, 07 Oct 2004 09:58:33 -0500
} To: MICHAEL J DELANNOY {delannoy-at-jhmi.edu}
} From: Tom Phillips {phillipst-at-missouri.edu}
} Subject: [Microscopy] Re: Re: re: Hepes and reduction
} Cc: microscopy
}
} I have seen HEPES react with KMnO4 (it literally turns the mix into a gel
} that then breaks down into time into a precipitate). Never had a problem
} with either 1% osmium or 1% reduced osmium. After osmium, we usually
} rinse with dH2O before ethanol dehydration since by this time the tissue
} is no longer osmotically sensitive. We have done uranyl acetate in
} ethanol, or sodium acetate buffer, or water at this stage. I haven't seen
} a lot of difference but I think others are strong advocates of uranyl in
} the ethanol or sodium acetate. If by post-embedding IEM you mean doing
} immunostaining of osmicated, embedded tissues, you have a low probability
} of success with most antigens in my experience. I have had a few antigens
} survive osmication but generally only ones in high abundance to
} start. Most immuno EM work using staining of sections would use aldehyde
} fixation (formaldehyde only, or with low glutaraldehyde ~0.2% or sometimes
} a standard 2% PF + 2.5% glut) followed by buffer rinses and then maybe a
} uranyl acetate en bloc staining and dehydration before embedding in an
} immuno friendly plastic like K4M, HM20, LRW, or LRG. LR Gold is my
} current favorite but we find the choice is dependent on the antigen and we
} need to re-invent the wheel each time and try them all. During our
} immunostaining of sections, we use a HEPES buffer (70 mM NaCl, 30 mM
} HEPES, 2 mM CaCl2, pH 7.4) for the antibodies (usually with 0.1%
} BSA). good luck.
}
}
}
} At 09:12 AM 10/07/04, you wrote:
} } Tom,
} } I once read on this listserver that organic
} } buffers shouldn't be used with osmium because they
} } could react (oxidized?). Although I will admit that
} } I have used reduced osmium with hepes with no noticeable artifacts (maybe
} } due to the lower oxidation
} } state). I want to know if Hepes can be used as the
} } only buffer in a post-embed IEM protocol up to
} } uranyl acetate.
} }
} } thanks
} } Mike Delannoy
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 10:17:53 2004

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Silicon wafers behave pretty much like glass. They are ultra-smooth, cells
can be grown on them
without prior coating with gold, and they are rsistant to most reagents you
are likely to use.
An advantage of silicon as a substrate for SEM of cell monlayers is that the
substrate has some conductivity,
the silicon background charges rather less than glass for a given
accelerating voltage and beam current,
and therefore thinner sputtered coatings of gold/palladium, platinum or
chromium can be employed.
A disadvantage is they are more expensive than coverslips, and thicker.
Chris

----- Original Message -----
} From: "Garber, Charles A." {cgarber-at-2spi.com}
To: "MICROSCOPY BB" {Microscopy-at-MSA.Microscopy.com}
Sent: Thursday, October 07, 2004 3:04 PM

Continuing the thread, Some have cited acid-based markers as a potential
problem. Paul Beauregard offered a site at Sanford listing their acid-free
markers. It was not clear whether Sharpie Fine Point markers were included
or not, so I posed the question to their customer service department asking
for clarification. I received the following two responses.

First, they stated that their markers are not acid-free. Next, they stated
that their markers have been tested on CDs without problems.

I guess that simply means that no problem has been observed yet. Since it
is a case of trying to prove a negative, I will go ahead and continue to
use them unless someone can definitely show that the Fine Point markers
have caused a problem. But I suppose I will still pick up a 'certified' CD
marker the next time I go to the store.

Warren

} From: {consumer.service-at-sanfordcorp.com}
} To: {wesaia-at-iastate.edu}
} Subject: [Microscopy] Re: 000356732A, reply from Sanford, Paper Mate, Sharpie,
} uni-ball, Eldon Office Solutions or Foohy.com web-site.
} Date: Tue, 5 Oct 2004 19:00:25 -0400
}
} Hello Warren,
}
} We received your recent inquiry regarding Sharpie� Fine Point Markers.
}
} The Sharpie� Fine Point Markers are not acid free.
}
} Thank you for e-mailing us and for your support of Sanford products.
}
} Sanford Consumer Affairs
}
} 000356732A

} From: {consumer.service-at-sanfordcorp.com}
} To: {wesaia-at-iastate.edu}
} Subject: [Microscopy] Re: 000356732B, reply from Sanford, Paper Mate, Sharpie,
} uni-ball, Eldon Office Solutions or Foohy.com web-site.
} Date: Thu, 7 Oct 2004 11:00:05 -0400
}
} Hello Warren,
}
} Although the Sharpie Markers are not acid free, they have been found safe
} for use on CD's and DVD�s by our lab. Our lab has conducted extensive
} testing on these markers, and found since they are alcohol based, they are
} safe for that application.
}
} Thank you for e-mailing us and for your support of Sanford products.
}
} Sanford Consumer Affairs
}
} 000356732B
-------------
} Date: Mon, 04 Oct 2004 20:41:11 -0400
} To: Microscopy-at-microscopy.com
} From: Beauregard {beaurega-at-westol.com}
} Subject: [Microscopy] Re: Re: CD/DVD as archival media revisited
}
} Hi,
}
} I agree with Henk and Mike. I believe CDRs ARE archival grade if handled
} properly. Even those thousand year old clay tablets, suggested in jest,
} are almost useless if broken or turned to dust from improper handling.
}
} I did not read the whole article but I what to address the Sharpie� acidic
} ink problem. In the interest of having all this CDR stuff in one posting,
} I offer this web information:
}
} I used Sharpie pens for years on CDRs without a problem.
} The acidic pen posting raised these questions:
} Were my Sharpie� pens the acidic types?
} Was my first official US flag research CD going to disintegrate?
} So, I looked to SandfordCorp.com to see what they said about acidic pens.
}
} http://www.sanfordcorp.com/sanford/consumer/jhtml/help/sanford_help_922.jhtml
}
} Paul Beauregard
} Senior Research Associate

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 12:24:33 2004

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A colleague is interested in using a centrifuge to spin cells down on to
glass slides. We would like the cells to be in multiple discrete spots on
the slide. Anybody have tips on using this approach or places to buy such
a device. Thanks, Tom

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 12:47:33 2004

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Hi Listers:

I have a question regarding the ultrastructural differences between
Aspergillus and a gram positive bacteria. Does anyone out there know any
differences that could be documented by the electron microscope? We have
done all the special stains on this pathology specimen, but we are hoping
the EM will help to further identify the organism we have in question.

Thanks for any help!

Karen L. Bentley, M.S.(previously Jensen)
Associate Scientist & Project Manager
Electron Microscope Research Core
University of Rochester Medical Center
Rochester, NY 14642
585-275-1954

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 13:33:44 2004

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ThermoElectron Cytospin:
http://www.thermo.com/com/cda/product/detail/1,,16469,00.html

Up to two 6-mm diameter cell spots per cytofunnel. I authored the user's'
manual in 1982 or so.

Gary Gill

-----Original Message-----
} From: Tom Phillips [mailto:phillipst-at-missouri.edu]
Sent: Thursday, October 07, 2004 12:29 PM
To: Microscopy-at-msa.microscopy.com

NESM Fall 2004 Meeting and Workshop on Cryo Techniques

The 2004/2005 meetings of the New England Society for Microscopy kick off this
Fall with a pair of events on Cryo Techniques for specimen preparation. These
techniques are useful for processing soft, high-volatile specimens for both SEM
and TEM investigations. Soft, wet specimens are common in biological and human
medical investigations. Perhaps less-appreciated are the relatively dry, soft
polymers investigated by materials scientists and food technologists. The
introductory event consists of three lectures on Cryo Techniques in Microscopy.
It will be held on October 19th in the JEOL conference room in Peabody,
Massachusetts. Registration starts at 5:30 PM, then a buffet dinner, and then
the presentations. Those especially interested in learning Cryo skills should
also plan to attend the two-day Workshop on Cryo-Ultramicrotomy on November 3-4.
This workshop, sponsored or supported by NESM, RMC/Boeckeler, Harvard, and MIT,
will be held at Harvard's Center for Imaging and Mesoscale Structures. The
details of the meeting and workshop, including registration information, can be
found on NESM's website http://prism.mit.edu:8083 under "current newsletter".

Registration forms/information for both events can be sent to: NESM Treasurer,
Paul Bain c/o NESM, Harvard Medical School, Countway 212, 10 Shattuck Street,
Boston, MA 02115 email: Paul_bain-at-hms.harvard.edu. The October 19th meeting
costs $5 for members and $20 for non-members (you receive a one-year NESM
membership) and the registration deadline is Friday, October 15th. The November
3-4 Workshop has a $50 professional registration and a $25 student registration.
The registration deadline for the Workshop is Friday, October 22nd. (Register
early for the workshop--space is limited!)

Peggy Sherwood
Corresponding Secretary/Newsletter Editor-NESM

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

From MicroscopyL-request-at-ns.microscopy.com Thu Oct 7 16:40:44 2004

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karen

while you are aware of this, we should all remind ourselves that EM
diagnostics of micro-organisms can be quite inexact. while negative
stain of viruses will usually give good information concerning the
family, even there we cannot go beyond the fact that the virus is a
herpes, not a poxvirus, not that it is cytomegalovirus, or herpes
zoster, or any other herpes, for that matter. we need the information
of the infectious diseases and/or microbiology staff to be able to go
from EM to specific diagnosis.

having said that, there are clear differences between the bacterial and
fungal cells. most significant is the presence of internal membrane
organelles with the eukaryotic cell, such as aspergillis, and the
presence of the nucleoid in the prokaryotic cell. a second difference
is that the fungal cell is significantly larger than the bacterial
cell. by this, i mean 15-30microns as opposed to 1-5 microns. thirdly,
the yeast and fungal cell walls and external structures are thicker (up
to 500nm) than those seen with the gram positive bacteria. fourthly,
with yeast, and some fungi, you will see budding scars. their
orientation may give some idea as to the specific nature of the
micro-organism you are seeing.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 8 01:12:32 2004

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Hi

We are looking at upgrading our facility here for photographing resected
organs etc at the histopathology department. The new system will be based
on digital cameras so that we can link the photos to the patient
information and diagnosis in our database.

What I would like to know is what people are using these days in terms of
macro lenses, ring flashes (if they exist for the newer cameras) and
lighting systems, lamps, multiple swan-neck lighting.

Any tips would be appreciated from users and from manufacturers etc.

Thanks

Med v�nliga h�lsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Bes�ksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
f�r klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 8 03:25:57 2004

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Hi,

Thank you all for sending information and suggestions about calcium
ratio mesurements. The main issue was/is to find a camera-solution to
achieve at least the the same framerate as was possible with the old
hardware (now obsolete). Commonly used PAL video-cameras, have a
framerate of about 25 frames per second (FPS, or 33 in NTSC countries)
or lower. Popular digital cameras have advantages over "traditional"
video-standard cameras, but not always achieve a framerate of 25/33 FPS.

What was done in the old system, was to put a "temporal" framesplitter
on the camera, so it allowed to capture quarter frames at 1/4 of the
frametime, which lead to a temporal sampling of 10 msec instead of 40 msec.

Chosing a modern camera which surpasses the old days solution is not a
simple exercise. FireWire interfaces (800 Mbits/sec.) do not deliver the
high bandwidth of CameraLink interfaces (base 2380 Mbit/sec. up to 7140
Mbit/sec.) and their performance goes down when using full-frame
transfer. FireWire cameras are widely available, while the faster
CameraLink cameras are not so popular. Every computer nowadays comes
with a FireWire interface, but a CameraLink is not standard.

http://vsd.pennnet.com/Articles/Article_Display.cfm?Section=Articles&Subsection=Display&ARTICLE_ID=187955

Regards,

Peter

----------------------------------------------
Peter Van Osta

Director Imaging
MAIA SCIENTIFIC
Cipalstraat 3
B-2440 Geel, Belgium
Tel.: +32 (0)14 570 620
Mobile: +32 (0)497 228 725
Fax.: +32 (0)14 570 621
Email: pvosta-at-maia-scientific.com
Website: www.maia-scientific.com
A Harvard Bioscience Company
----------------------------------------------

=========================================================
Hi,

Already a couple of years ago, former colleagues did automated
calcium-ratio measurements with CCD-camera's on individual
(cardio)myocytes in cell cultures. They used a modified CCD-camera which
allowed them to capture an image of an individual (cardio)myocyte every
10 milliseconds to monitor intracellular calcium.

I am wondering which type of camera could now be used to give the same
time-resolution (10 msec. frame time) for individual cells (the hardware
they used is now no longer available) ?

Reference:

Cornelissen, F., Wouters, L., Ver Donck, L., Verellen, G., Geerts, H.
Automatic quantification of the effect of cardioprotective drugs in
isolated myocytes.
Bioimaging, 1993, 1, pp. 197-206.

Regards,

Peter Van Osta

----------------------------------------------
Peter Van Osta

Director Imaging
MAIA SCIENTIFIC
Cipalstraat 3
B-2440 Geel, Belgium
Tel.: +32 (0)14 570 620
Mobile: +32 (0)497 228 725
Fax.: +32 (0)14 570 621
Email: pvosta-at-maia-scientific.com
Website: www.maia-scientific.com
A Harvard Bioscience Company
----------------------------------------------

From MicroscopyL-request-at-ns.microscopy.com Fri Oct 8 06:04:17 2004

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Both Nikon and Olympus manufacture professional digital SLRs with
top-quality macro lenses and flash systems.
I always used to rate Olympus 35mm systems (based on OM2n, OM4ti) highly for
their macro lenses, and TTL ringflash and macro-flash capability, and had
always dreamed of the day when they would issue their updated system. Alas
that was not to be, but an interesting new digital system has been launched
by Olympus called E1 which uses the four thirds system. Macro lenses and a
ringflash are available for this camera, I see, so this may turn into the
digital equivalent of the OM system if it is equally well received in the
market place.

Depending on how critical you are of issues like camera versatility,
performance etc. you might get a long way towards a solution with a simpler
camera like the Nikon Coolpix 4500. It has remarkable macro capability for
the price, focussing to about 1 inch from front of lens, and there is a
so-called ringflash accessory for it too, actually a ring of high-intensity
white LEDs. I use one of these myself, and know many other scientists who
swear by them for field-recording of specimens ranging from fluorescent gels
to plants and fungi, even aurora borealis displays are within its
capabilities.

I have nothing to gain commercially from these comments
Hope this helps
Chris

----- Original Message -----
} From: "Gareth Morgan" {Gareth.Morgan-at-labmed.ki.se}
To: {Microscopy-at-msa.microscopy.com}
Sent: Friday, October 08, 2004 7:17 AM

For macrophotography of wet biological samples, you must use a
cross-polarization setup to eliminate unsightly lighting "glints"
(highlights). For the past 3 years, we have used both a copystand and
handheld configurations using:

Fujifilm S1 Pro DSLR (ISO 320, 3Kx2K pixels, JPG FINE setting)
Nikon 105mm MACRO with circular polarizing filter
Nikon Speedlight SB-29 Ring light with Polarizing filter
Nikon 24-140mm Zoom (general photography)
Bencher Copymate II with polarizing filters
IBM 1GB Microdrive
~1 ft. cable release (for use with copy stand configuration--don't get a
camera without cable release connector!)
Microdrive/Compact Flash USB Card Reader (forget about transferring directly
from camera to computer).

1. This older Fujifilm camera (current model is S3) is 3.3M sensor creating
6M image via interpolation. I've read and heard all the arguments regarding
interpolation, but the proof is in the image. Awesome. Camera will save in
TIF (18MB file) but it takes nearly 1 minute to transfer captured image to
microdrive so we opted for JPG format in FINE mode, so that the resulting
~2.5MB JPG format saves quicker, but still opens to 6Mpixel, 18MB image.
RAW format is way to go now, but the logistics of easily viewing RAW are not
available to most, and probably not handled by your database.

2. With copystand configuration and 105mm macro, adjust the circular
polarizer to minimize glints. Our current exposures are approximately 1/4 -
1/2 second, and really depend on tissue coloration.

3. For handheld configuration, one needs to determine the correct rotational
placement of the ringlight/polarizer relative to the polarizing filter on
the lens and make a series of test shots (this is because you can't see a
live preview of glint minimization as you do with the copystand setup). The
polarizing filter was custom cut to the shape of the ringlight and mounted
to the front.

Finally, the quality is primarily the lens and the sensor. Have some vendors
let you demo their DSLR models, if possible.

Best regards,

Walter F. Bobrowski
Investigative Pathology
Safety Sciences
Pfizer Global Research & Development
Ann Arbor, MI 48105

TEL: 734-622-7814
FAX: 734-622-3478
Mobile: 734-646-0502

-----Original Message-----
} From: Gareth Morgan [mailto:Gareth.Morgan-at-labmed.ki.se]
Sent: Friday, October 08, 2004 2:17 AM
To: Microscopy-at-msa.microscopy.com

Hi

We are looking at upgrading our facility here for photographing resected
organs etc at the histopathology department. The new system will be basedo
n digital cameras so that we can link the photos to the patient
information and diagnosis in our database.

What I would like to know is what people are using these days in terms ofm
acro lenses, ring flashes (if they exist for the newer cameras) and
lighting systems, lamps, multiple swan-neck lighting.

Any tips would be appreciated from users and from manufacturers etc.

Thanks

Med v�nliga h�lsningar/With best regards

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Bes�ksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratorietf
�r klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.

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