Microscopy ListServer Archives  


File Requested = 0504.txt
Retrival Software Version=NJZ07060908

From: Beth Richardson :      beth-at-plantbio.uga.edu
Date: Fri, 1 Apr 2005 10:04:06 -0500
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

On my bulletin board I have a wonder photo of Katherine Esau - she is
standing next to a TEM (column) and the caption says "Modern American
Gothic".

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I have had a request for information on women who were on the forefront
} during the early days of development of electron microscopy. Of most
} interest would be those who were involved in Biological imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}
**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 09:38:40 2005



From: Scheltens Frank J Contr AFRL/MLLN :      Frank.Scheltens-at-wpafb.af.mil
Date: Fri, 1 Apr 2005 10:28:19 -0500
Subject: [Microscopy] Electron Microscopy Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopy Technician


UES Incorporated, a high-tech R&D company in Dayton, Ohio and operator of the AFRL Materials Characterization Facility (MCF), has an immediate need for a full time electron microcopy research technician. The candidate must have a minimum of three to five years experience, a High School degree (Associates degree preferred) and be capable of providing on-site support at the MCF located within WPAFB AFRL/Materials and Manufacturing Directorate. The ideal candidate will be experienced in the operation and maintenance of TEM, SEM and associated support equipment as well as the preparation of materials for examination by these techniques. Experience with the electron microscopy of high temperature ceramic, intermetallic, metallic, metal matrix composite and ceramic matrix composite materials, as well as their preparation by metallographic, electropolishing and ion milling techniques is highly desirable. Knowledge of polymer, Carbon / Carbon composite and semi-conductor electron m!
icroscopy and the preparation of these materials, including experience in microtomy of materials, is preferred. The technician must also be capable of training and assisting individuals in the execution of all of the above techniques. Additionally, they will be responsible for the maintenance of the electron microscopes, which are all under service contract, and the maintenance of associated support equipment such as sample preparation facilities, as well as other general laboratory duties. U.S. Citizenship is required.


UES, Inc. Attn: Debbie Yount 4401 Dayton-Xenia Road Dayton, OH 45432 Fax: (937) 426-0533; E-mail: humanresources-at-ues.com; AA/EEO Employer





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 10:49:25 2005



From: Ronald Smith :      rsmith-at-uwo.ca
Date: Fri, 01 Apr 2005 12:05:46 -0500
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debby,
Just a couple of Canadians that come to mind are Dr.Bessie Borwein,
Dept. of Anatomy University of Western Ontario, Dr Elaine Humphrey
University of British Columbia, vancouver B.C. Dr. Margaret McCully,
Carlton University, Ottawa.

Ron.
Debby Sherman wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Ronald J. Smith
Department of Biology
Room 235, Biological & Geological Sciences Bldg.
U.W.O., London, Ontario
N6A 5B7
(519) 661-2111 ext.86486




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 11:10:44 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Fri, 01 Apr 2005 12:19:51 -0500
Subject: [Microscopy] Re: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This would make a great dissertation topic for a History of Science
student. MSA should consider funding such a student to go around and
interview these women who are still alive and close associates of those who
have gone on to the great EM lab in the sky.

At 10:04 AM 4/1/2005, Beth Richardson wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 11:19:14 2005



From: Salamon, Daniel :      Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Fri, 1 Apr 2005 12:31:59 -0500
Subject: [Microscopy] checking for sample contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

It seems that people are putting all the wrong samples into our field emission SEM and it's causing contamination problems. We decided to ask all people to fill in forms with detailed information about their samples, but it's hard to predict how all samples will behave in vacuum.

1. Can you suggest a way to check that samples are vacuum-friendly before loading them into the SEM?

2. Is there any good way to periodically clean the specimen chamber from contamination?

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-081 ECERF Bldg
9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 11:43:46 2005



From: BETTY FABER :      bfaber-at-lsc.org
Date: Fri, 01 Apr 2005 12:54:46 -0500
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Eleanor H. Slifer

She did insect chemoreceptors.

Academy of Natural Sciences in Philadelphia


cheers

Betty Faber
Liberty Science center

} } } Beth Richardson {beth-at-plantbio.uga.edu} - 4/1/05 10:04 AM } } }


------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

On my bulletin board I have a wonder photo of Katherine Esau - she is

standing next to a TEM (column) and the caption says "Modern American

Gothic".

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
}
-----------------------------------------------------------------------

} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------

} --------
}
} I have had a request for information on women who were on the
forefront
} during the early days of development of electron microscopy. Of
most
} interest would be those who were involved in Biological imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}
**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************

***





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 13:20:30 2005



From: Ted Pella :      ted_pella-at-tedpella.com
Date: Fri, 1 Apr 2005 11:33:32 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Debbie and Caroline,

I can't remember the lady who, in the early days, worked at Rockefeller
and married Dr. George Palade. It seemed to me that she was very active
in the early research days. She finished her career at the Pathology
Department at the Univ. of Calif. Medical School in San Francisco. I'll
try to recall that name.

I agree that Audrey Glauert is a terrific choice.

Ted Pella



-----Original Message-----
} From: Caroline Schooley [mailto:schooley-at-mcn.org]
Sent: Thursday, March 31, 2005 2:43 PM
To: Debby Sherman
Cc: Microscopy-at-MSA.Microscopy.Com

} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} during the early days of development of electron microscopy. Of most
} interest would be those who were involved in Biological imaging. I drew

} a blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby -

How early is early? 1950s? The wonderful protozoologist who gave me
my first EM job at U.C. Berkeley in '53, Dorothy Pitelka. I watched
her make glass knives by throwing a piece of plate glass on the floor
& picking thru the shards. And certainly Audrey Glauert, who is
still with us; ask her for more names: amg44-at-cam.ac.uk

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates:
http://www.fortbragg.k12.ca.us/AG/marinelab.html






From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 13:22:33 2005



From: Ted Pella :      ted_pella-at-tedpella.com
Date: Fri, 1 Apr 2005 11:35:38 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

How could I forget!! Talk about lapses.

Chris and I visited with Norma Reid (who wrote "Sectioning" in the
Glauert series)in February, in New Zealand - and with her husband Bob
Hudson.

As far as I am concerned, Judy and Caroline are qualified for the
incredible teaching, scrimping and saving under tough budgeting
conditions while teaching a large number of individuals over years.
And, in article submissions.




-----Original Message-----
} From: Judy Murphy [mailto:murphyjudy-at-comcast.net]
Sent: Thursday, March 31, 2005 4:13 PM
To: Debby Sherman; microscopy-at-msa.microscopy.com

Hi Debby,

Also Norma Reid
bob.norma-at-bopis.co.nz

and
Chris Pella for contacts of who was there
contact at Ted Pella Inc.

Good Luck
There were actually quite a few women involved.

Judy

Debby Sherman wrote:

} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} during the early days of development of electron microscopy. Of most
} interest would be those who were involved in Biological imaging. I drew

} a blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
}
}
}
}
}
}






From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 14:02:34 2005



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 1 Apr 2005 10:14:47 -1000 (HST)
Subject: [Microscopy] Re: checking for sample contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi-

I just dealt with this, probably as you were hitting the "send" button.
Someone came in with charcoal that they said probably had 20% humidity. We
have a Hitachi FESEM with a pre-pump chamber/airlock and if something
takes longer than 30 sec to pump, it's too wet or gassy. So I pumped it in
the sputter coater to 30 mTorr for a few minutes, then put it in the
pre-pump chamber for a bit, then there was no problem.

For contamination, I question people closely about their samples. Covered
in oil? No way. Some things I'll sneak a quick peek at to see what
happens. Stuff embedded in epoxy and then polished, I'll warn them not to
let the beam touch the epoxy. Preview anything suspicious and ban it if
it's going to muck up the column!

} It seems that people are putting all the wrong samples into our field emission SEM and it's causing contamination problems. We decided to ask all people to fill in forms with detailed information about their samples, but it's hard to predict how all samples will behave in vacuum.
}
} 1. Can you suggest a way to check that samples are vacuum-friendly before loading them into the SEM?
}
} 2. Is there any good way to periodically clean the specimen chamber from contamination?

Good luck,
Tina


****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 15:35:33 2005



From: Ted Pella :      ted_pella-at-tedpella.com
Date: Fri, 1 Apr 2005 13:48:41 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Yes Debbie, Marilyn Farquhar was the woman I was thinking of - married
to Dr. Geo. Palade, Rockefeller-Yale-UCSFMedSch

Ted

-----Original Message-----
} From: Marc Pypaert [mailto:marc.pypaert-at-yale.edu]
Sent: Thursday, March 31, 2005 2:44 PM
To: Debby Sherman
Cc: message to: MSA list

How about Marilyn Farquhar, who used to work
here at Yale with another great pioneer of
cell biology and EM - George Palade. She
published some influential EM papers
in the 50's already.

Marc

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
} ----------------------------------------------------------------------
} -
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------
} --------
}
} I have had a request for information on women who were on the
} forefront during the early days of development of electron microscopy.

} Of most interest would be those who were involved in Biological
imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446







From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 16:19:15 2005



From: Sandra Masur :      sandra.masur-at-mssm.edu
Date: Fri, 01 Apr 2005 17:28:12 -0500
Subject: [Microscopy] Re: RE: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Marilyn Farquhar
and she is still active at UCSD - her career is not finished!

On Friday, April 1, 2005, at 02:33 PM, Ted Pella wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Greetings Debbie and Caroline,
}
} I can't remember the lady who, in the early days, worked at Rockefeller
} and married Dr. George Palade. It seemed to me that she was very
} active
} in the early research days. She finished her career at the Pathology
} Department at the Univ. of Calif. Medical School in San Francisco.
} I'll
} try to recall that name.
}
} I agree that Audrey Glauert is a terrific choice.
}
} Ted Pella
}
}
}
} -----Original Message-----
} } From: Caroline Schooley [mailto:schooley-at-mcn.org]
} Sent: Thursday, March 31, 2005 2:43 PM
} To: Debby Sherman
} Cc: Microscopy-at-MSA.Microscopy.Com
} Subject: [Microscopy] Re: Women pioneers in electron microscopy
}
}
}
}
} -----------------------------------------------------------------------
} -
} ------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} -
} -------
}
} } ----------------------------------------------------------------------
} } -
} } -------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } -
} --------
} }
} } I have had a request for information on women who were on the
} } forefront
}
} } during the early days of development of electron microscopy. Of most
} } interest would be those who were involved in Biological imaging. I
} } drew
}
} } a blank as all the early pioneers that came to mind were men.
} }
} } Help me out here!
} }
} } Debby -
}
} How early is early? 1950s? The wonderful protozoologist who gave me
} my first EM job at U.C. Berkeley in '53, Dorothy Pitelka. I watched
} her make glass knives by throwing a piece of plate glass on the floor
} & picking thru the shards. And certainly Audrey Glauert, who is
} still with us; ask her for more names: amg44-at-cam.ac.uk
}
} Caroline
} --
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
} Intertidal invertebrates:
} http://www.fortbragg.k12.ca.us/AG/marinelab.html
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 17:01:38 2005



From: Webster, Paul :      PWebster-at-hei.org
Date: Fri, 1 Apr 2005 15:12:55 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ted,

A small correction is needed here. Professor Farquar is still based in San Diego (UCSD), where she moved to when she left Yale.

No-one has mentioned Betty Hay yet. She ran the Anatomy Department at Harvard Medical School for many years. This department was very influential in sponsoring electron microscopy for biologists. I can think of a few names that have links with this department (Fawcett, Ito, Karnovski). I am sure there are more in this list. I think I once saw a photo of a young Ted Pella looking down an AEI microscope that may have been in Harvard.

Is it true that the early TEMs used rubber mallets to align the apertures?

Paul Webster.

-----Original Message-----
} From: Ted Pella [mailto:ted_pella-at-tedpella.com]
Sent: Fri 4/1/2005 1:48 PM
To: 'Marc Pypaert'; 'Debby Sherman'
Cc: 'message to: MSA list'

Yes Debbie, Marilyn Farquhar was the woman I was thinking of - married
to Dr. Geo. Palade, Rockefeller-Yale-UCSFMedSch

Ted

-----Original Message-----
} From: Marc Pypaert [mailto:marc.pypaert-at-yale.edu]
Sent: Thursday, March 31, 2005 2:44 PM
To: Debby Sherman
Cc: message to: MSA list

How about Marilyn Farquhar, who used to work
here at Yale with another great pioneer of
cell biology and EM - George Palade. She
published some influential EM papers
in the 50's already.

Marc

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
} ----------------------------------------------------------------------
} -
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------
} --------
}
} I have had a request for information on women who were on the
} forefront during the early days of development of electron microscopy.

} Of most interest would be those who were involved in Biological
imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446












From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 18:35:18 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Fri, 01 Apr 2005 18:47:40 -0600
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Karnovsky was in the Pathology dept not Anatomy. I once asked Sandford
Palay (Anatomy at HMS) in the late 80's about using TEM's in the "old" days
and he told me how they used rubber mallets to align the apertures. they
just tapped on the column until they fell into the correct location. I am
not sure but I think it was Fawcett who hired Ito and Hay and then Betty
Hay went on to assume Fawcett's position when he retired.





At 05:12 PM 04/01/05, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 18:42:51 2005



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 1 Apr 2005 17:00:01 -0800
Subject: [Microscopy] RE: RE: Re: Women pioneers in electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Paul -

I guess I AM a pioneer, because I've seen this. Dorothy Pitelka used
Tom Hayes' RCA EMU 2 at what is now Lawrence Berkeley Lab in the mid
50s. That scope had a "fixed" aperture & owners loosened the
clamping screws. Tom used a ball peen hammer - gently.

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 19:37:51 2005



From: Narasimhan S :      narasimhanpotti-at-hotmail.com
Date: Sat, 02 Apr 2005 01:50:36 +0000
Subject: [Microscopy] Star Anaphase & Chromosome stickiness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Can anybody tell me what is chromosome stickiness and star anaphase ?
How do they occur ?

thanks all,
Narasimhan

_________________________________________________________________
The MSN Survey!
http://www.cross-tab.com/surveys/run/test.asp?sid=2026&respid=1 Help us help
you better!



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 19:51:06 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Fri, 01 Apr 2005 18:02:52 -0800
Subject: [Microscopy] Re: RE: RE: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We stigmated (objective lens) our RCA 2A and 2E with a rubber mallet for
yrs - very gently!!! It moved the aperture around enough to affect the
astigmatism.

We had the 2nd Cambridge SEM in the country Mark I and did several
things with that with a rubber mallet!!!!!

Judy Murphy



Caroline Schooley wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} }
} } Is it true that the early TEMs used rubber mallets to align the
} } apertures?
} }
} } Paul Webster.
}
}
} Paul -
}
} I guess I AM a pioneer, because I've seen this. Dorothy Pitelka used
} Tom Hayes' RCA EMU 2 at what is now Lawrence Berkeley Lab in the mid
} 50s. That scope had a "fixed" aperture & owners loosened the clamping
} screws. Tom used a ball peen hammer - gently.
}
} Caroline



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 21:57:59 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Fri, 01 Apr 2005 22:16:54 -0600
Subject: [Microscopy] Re: RE: RE: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

paul

unlike carolyn, my first experience was with an old Siemens, they used a
soft brass mallet, not a ball peen hammer. i say they - a young
technician trainee would never have been allowed to touch the beast.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 2 00:50:13 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Fri, 01 Apr 2005 23:02:27 -0800
Subject: [Microscopy] Re: Re: RE: RE: Re: Women pioneers in electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Siemens I: Like Paul's lab, most weren't allowed to tinker with the
Siemens I as it was the "research microscope" in the early 60's at the
Bevier Hall Center for Microscopy at Univ of Illinois (Urbana). ALL of
us users however were many times tempted as The bathroom was a couple
doors away down the hall. EVERYTIME someone flushed the toilet, the
Siemens "red barred" which meant it got a signal for low water pressure,
and went through its vacuum sequencing and one had to manually valve it
back to high vacuum which took a long time. It wasn't very conducive
for "research microscopy" or any kind of microscopy on busy lab days
(i.e. when lots of folks used the bathroom).

Can't resist sharing this story also about the Siemens: apologize for
the aside ahead of time.
The Siemens was in a room (column only, power supplies in separate room)
about 4 ft x 5 ft or very small. The Electronics Engineer in the lab
decided one morning to bring his son's 10 ft boa constrictor into lab
(haven't a clue why, but anyway he did) and decided he wanted it
contained in a small room i.e. the Siemens room (smallest room in the
set of labs). He had come in at 5:30am and put it in the room. The
snake of course liked the warmth near the diffusion pump at the front
bottom of the column. I had the Siemens that day from 6am to 6pm. Got
the sample loaded, and turned out the lights (the snake being out of
sight underneath). Well you can imagine my surprise when something
crawled around my legs which were under the scope. After running out of
the room, and likely screaming at the top of my voice, the Siemens took
on a whole different meaning besides microscopy!!!!! Later, much
later,it was determined that the engineer knew I had the scope at 6, and
thus planned to have it in there when I arrived. ALSO, I didn't
appreciate the humor in it all until a GREAT deal of time passed! AND
yeah, before anyone asks, after that I didn't like snakes much,
especially crawling on me.

Judy



Judy Murphy, PhD
Microscopy Training, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net


paul r hazelton wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 2 19:24:26 2005



From: Corvos-at-aol.com
Date: Sun, 3 Apr 2005 17:51:27 EDT
Subject: [Microscopy] Vacuum Pump service Houston TX area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Well, I guess I have to add the name of Elizabeth MacLean, who was my
guide in a course in Optical Methods in Biology at Brandeis during
1963. I remember not only the optical theory, but also the aroma of
floating colloidion films out of amyl acetate.

Send reply to: {ted_pella-at-tedpella.com}
} From: "Ted Pella" {ted_pella-at-tedpella.com}
To: "'Caroline Schooley'" {schooley-at-mcn.org} ,
"'Debby Sherman'" {dsherman-at-purdue.edu}
Copies to: {Microscopy-at-MSA.Microscopy.Com}

All,

Does anyone know of a Edwards vacuum pump service company in the Houston TX
area.

Regards,

Walter Protheroe
E-MAC, Inc
corvos-at-aol.com
www.emaci.com



From MicroscopyL-request-at-ns.microscopy.com Sun Apr 3 21:38:13 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Sun, 03 Apr 2005 21:52:22 -0500
Subject: [Microscopy] Women EM Pioneers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My request for names of women who were pioneers in the field of electron
microscopy has resulted in a extensive list. The list starts with those
active in the 40¹s and continues on to many women who are still very active
in the field today. Their inclusion in the list is due to their influence
on someone who took the time to honor them by responding to my initial
request.

I have sorted out those that I knew were active in the very early days.
These women were indeed pioneers in both scientific research, when the
numbers of active women were rather small, and in the new discipline of
electron microscopy. In addition there is an extensive list of others who
carried on with the research using EM and helped expand or open new areas of
research.

Thanks to all who replied and I apologize if I left someone off of the list.
It was not intentional but rather a matter of trying to do to many things in
too short of an amount of time.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


Women in the early 40¹s
Beatrice Deacon and Alice Gray (Toronto, E.F Burton group)
Katherine Polevitsky (Bacteriology, U. of Penn.)
Irene Manton FRS: very early plant electron microscopist who worked
primarily at Leeds University in the UK. She held the Chair of Botany there
from 1946 - 1969, and died in 1988. A building at Leeds University is now
named after her as well as prize from the Linnean Society and Phycological
Society



mid to late-40s
Mary Schuster Jaffe: formvar fenestrated films (first worked with E.F.
Fullam)
Gertrude Fleming Rempfer: Electron optics -- one of the best, man or woman

Early 50¹s
Jean Hanson (E.J. Hanson) who first showed the structure of actin
microfilaments.
Marie Jakus: Biological EM (collagen & muscle proteins) with F.O. Schmitt
and Cecil Hall at MIT
Audrey Glauert: originally trained at the Rockefeller Cytology Group
(Porter and Palade) along with Farquar and Bonnneville
Marilyn Farquhar (Yale)
Mary Bonneville


EMSA Education Committee members in the early years (with most still active)
Caroline Schooley
Judy Murphy
Betty Mathews
Beverly Giammara

June Almeida, Francis Doane and Nan Anderson: Microbiology, esp virology

Katherine Esau: light microscopist who entered the field of EM to better
elucidate plant structure, and plant pathogen/host systems. Member of Nat¹l
Academy of Science

Elizabeth (Betsy) Haye: Head of Anatomy, Harvard School of Medicine

Dorothy Pitelka: protobiologist..Berkeley

Chris Pella

Helen Padykula (Mt. Holyoke 1948, Harvard 1954) the American Society of Cell
Biology¹s second woman president

Geraldine Gauthier, a student of Helen Padykula, was at Brown, Harvard,
Wellesley and U. Mass. Med. from the 60s to the 80s.
Norma Reid: wrote "Sectioning" in the Glauert series

Dr. Betty Geren Uzman, Mary Jaffe, and Marie Jakus.

Eleanor H. Slifer: insect chemoreceptors. Academy of Natural Sciences in
Philadelphia

Elizabeth Luduc,cro-TEM sectioning methods (1960s)

Agnes Oberlin: electron microscopist in France (TEM) going back to the 40s.
carbon-based materials

Helen Gay: first of cold Spring Harbor and later The
University of Michigan? In the mid 1950's she published beautiful
micrographs of cell nuclei and nucleocytoplasmic relationships --"blebs".





From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:46:03 2005



From: somayyeh_kheiri-at-yahoo.com (by way of Ask-A-Microscopist)
Date: Mon, 4 Apr 2005 09:02:10 -0500
Subject: [Microscopy] AskAMicroscopist: FAA preserved materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, April 3, 2005 at 00:13:14
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh kheiri

Organization: urmia university

Education: Graduate College

Location: Urmia,west azarbaijan,Iran

Question: Hello Dear all

I have a question about FAA preserved materials:
I am doing experiments on ssectionig fruit pericarp and leaves.I have gathered the material in FAA
how long do you put the material in FAA?the epidermis in the fruits that i have preserved in FAA sometimes become detached from the mesocarp.
I thought may this problem be because of the long time of preservation i have kept the material.(6month)I thought the tissues have been loosened.

can it be the reason?

thank you very much
Somayyeh



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:47:53 2005



From: Bstud-at-yandex.ru (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:03:55 -0500
Subject: [Microscopy] viaWWW: Backscattered and secondary electrons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Bstud-at-yandex.ru) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 4, 2005 at 04:09:17
---------------------------------------------------------------------------

Email: Bstud-at-yandex.ru
Name: Andrey

Organization: IPM RAS

Title-Subject: [Microscopy] [Filtered] Backscattered and secondary electrons

Question: I'm doing some work in electron-beam lithografy and need to know: how to calculate, where backscattered and secondary electrons will occur. Please give me references to abstracts or computer programs which may be helpful for this.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:48:13 2005



From: Somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:04:23 -0500
Subject: [Microscopy] viaWWW: pollen grain counting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday, April 3, 2005 at 06:26:26
---------------------------------------------------------------------------

Email: Somayyeh_kheiri-at-yahoo.com
Name: Somayyeh

Organization: urmia university

Title-Subject: [Microscopy] [Filtered] about pollen grain counting

Question: Hello dear all

I have been counting the number of pollen grains and ovules to get the pollen/ovule ratio for verbascum species
and populations.

I pressed the anthers of the flowers and provided a susponsion of the debris of anthers in distilled water to count the pollen
grains in a 5 microliter volume.

some of the samples in the susponsion were counted
one day after they were provided so the result showed
differences from the first sample which was counted immidiately after providing the susponsion.
I myself thought keeping the material in water for oneday
caused the grains to be attached together and the amount of the grains that are counted are reduced.

can anybody help me solve the problem?

is this reasonable or there maybe errors in the method of counting?

I appreciate any kind reply.

Somayyeh



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:50:14 2005



From: vfink-at-shaw.ca (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:06:26 -0500
Subject: [Microscopy] viaWWW: Thanks to all for recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (vfink-at-shaw.ca) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 1, 2005 at 17:37:07
---------------------------------------------------------------------------

Email: vfink-at-shaw.ca
Name: Victoria

Title-Subject: [Microscopy] [Filtered] Thanks to all for recommendations

Question: I just wanted to thank you all for the making recommendations, and providing with useful information regarding to the used analytic techniques search.
It is amazing how helpful could be this professional collaboration networking!

Victoria

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:50:21 2005



From: narasimhanpotti-at-hotmmail.com (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:05:55 -0500
Subject: [Microscopy] viaWWW: chromosome stickiness & star anaphase

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (narasimhanpotti-at-hotmmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 1, 2005 at 19:40:04
---------------------------------------------------------------------------

Email: narasimhanpotti-at-hotmmail.com
Name: Narasimhan

Organization: University of Kerala

Title-Subject: [Microscopy] [Filtered] chromosome stickiness & star anaphase

Question: Dear all,

Can anbody tell me what is chromosome stickiness and star anaphase ? How do they occur in mitosis or meiosis ?

thanks,
Narasimhan

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:50:50 2005



From: corvos-at-aol.com (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:06:50 -0500
Subject: [Microscopy] viaWWW: Roughing pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (corvos-at-aol.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 1, 2005 at 17:02:43
---------------------------------------------------------------------------

Email: corvos-at-aol.com
Name: Walter J. Protheroe Jr.

Organization: Energy - Micro-Analytical Consultants, Inc.

Title-Subject: [Microscopy] [Filtered] MListserver: Roughing pumps

Question: I am looking for a company in the Houston, TX area that can rebuild Edwards roughing pumps. Any info would be a help...

Regards,

Walter Protheroe
E-MAC, Inc
corvos-at-aol.com
www.emaci.com

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 10:10:25 2005



From: glaevsky-at-ECE.NEU.EDU
Date: Mon, 04 Apr 2005 11:26:31 -0400
Subject: [Microscopy] Motorized Linear Positioner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

I'm looking for a motorized linear positioner that will be putting
different types of mirrors in and out of the beam path. X resolution is
not critical. Speed is a moderate concern. I need an approximate travel
distance of 16 cm. Weight is also not a concern.

I searched and found about 30 different places. Does anyone have any
experience with any product such as this?

Thanks in advance for your help.
Best,

Gary



Gary Laevsky, Ph.D.
Keck Facility Manager, CenSSIS
Northeastern University
302 Stearns
360 Huntington Ave.
Boston, MA 02115
voice(617) 373 - 2589 {br}
fax(617) 373 - 7783 {br} {br}

http://www.censsis.neu.edu

http://www.ece.neu.edu/groups/osl

http://www.keck3dfm.neu.edu



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 17:07:47 2005



From: GAFTUNE-at-A0L.COM (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 17:23:35 -0500
Subject: [Microscopy] viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (GAFTUNE-at-A0L.COM) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 4, 2005 at 12:14:11
---------------------------------------------------------------------------

Email: GAFTUNE-at-A0L.COM
Name: GERALD FISHER

Organization: NA

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I AM AN ENGINEER WHO IS INTERESTED IN THE TECHNIQUES USED INIDENTIFYING PARTICLES FILTERED ONTO FILTER PAPER USING AN OPTICAL MICROSCOPE. THE PARTICL SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON NOMINAL DIAMETER OR LENGTH.

THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING SOLUTIONS. THEY MIGHT TYPICALLY BE ACTIVATED CARBON, METAL FINES(LIKE ELEMENTAL NICKEL OR COPPER), FILTER MEDIA POWDERS, OR ATMOSPHERIC DIRT.

ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE QUANTITY OF EACH PRESENT?

CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 17:08:10 2005



From: diller-at-assisi.de (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 17:24:05 -0500
Subject: [Microscopy] viaWWW: EMSCOPE Sputtercoater SC 500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-assisi.de) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 4, 2005 at 13:52:55
---------------------------------------------------------------------------

Email: diller-at-assisi.de
Name: Stefan Diller

Organization: Photography

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear listeners,
I am looking for manuals and electronic layouts for an EMSCOPE
Sputtercoater SC 500 with carbon coater head SB 250.
Any help would be fine, especially a short explanation how to work with it...
I reach the first trip-point at 0,15 Torr but then nothing happens after pressing the start-button...

Best regards,
Stefan Diller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 18:17:53 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 05 Apr 2005 11:33:57 +1200
Subject: [Microscopy] EDS detector vacuum lifetime?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


How long should the vacuum (and thus the LN2 consumption and the resolution) of a
Be-window EDS detector last?

The LN2 consumption of ours rose to about 2.2 litres/day by the time it was two years
old, then it was sent back to the factory for a leak-check and a pump 'n' bake.

When we got it back, the consumption rate was about 1.3 litres/day, but after a further
twenty-two months, the consumption had risen to around 2 litres.day. During this time
the detector had been mounted on an SEM which was under vacuum 24/7, and had not
been allowed to warm up at all.

The consumption then continued to rise, and after a further six months, all under
vacuum and with no warmups, it ran out of LN2 over 3 1/2 days of Easter, indicating a
consumption rate of about 2.2 litres/day.

And now, after recooling, the consumtion is up to about 2.4 litres/day, and, of course,
the resolution has degraded by about 3 eV.

It seems to me that this detector has always had a slow leak, not through the window,
which the factory failed to find and fix both at the time of manufacture and when it was
returned to them.

The factory, however, maintains that, because it was leak-checked when it was
returned to them, that any problem must have developed subsequently.

Now I'm not a deeply cynical person, but it seems to me unlikely that twice the same
detector has developed vacuum problems within two years of leaving the factory.

I will be interested in the opinions of others about the history of this particular detector,
and also in the experience of others with the vacuum lifetimes of their EDS detectors.

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 18:54:09 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Mon, 4 Apr 2005 18:07:49 -0600
Subject: [Microscopy] viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gerald,

please contact me via email. We have a product for exactly that application.

Mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: GAFTUNE-at-A0L.COM [mailto:GAFTUNE-at-A0L.COM]
Sent: Monday, April 04, 2005 16:24
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (GAFTUNE-at-A0L.COM) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April
4, 2005 at 12:14:11
---------------------------------------------------------------------------

Email: GAFTUNE-at-A0L.COM
Name: GERALD FISHER

Organization: NA

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I AM AN ENGINEER WHO IS INTERESTED IN THE TECHNIQUES USED
INIDENTIFYING PARTICLES FILTERED ONTO FILTER PAPER USING AN OPTICAL
MICROSCOPE. THE PARTICL SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON
NOMINAL DIAMETER OR LENGTH.

THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING SOLUTIONS. THEY MIGHT
TYPICALLY BE ACTIVATED CARBON, METAL FINES(LIKE ELEMENTAL NICKEL OR COPPER),
FILTER MEDIA POWDERS, OR ATMOSPHERIC DIRT.

ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE QUANTITY OF EACH
PRESENT?

CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 20:16:37 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 4 Apr 2005 18:47:45 -0700
Subject: [Microscopy] Re: EDS detector vacuum lifetime?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 4, 2005, at 4:33 PM, Ritchie Sims wrote:

} How long should the vacuum (and thus the LN2 consumption and the
} resolution) of a
} Be-window EDS detector last?
}
} The LN2 consumption of ours rose to about 2.2 litres/day by the time
} it was two years
} old, then it was sent back to the factory for a leak-check and a pump
} 'n' bake.
}
} When we got it back, the consumption rate was about 1.3 litres/day,
} but after a further
} twenty-two months, the consumption had risen to around 2 litres.day.
} During this time
} the detector had been mounted on an SEM which was under vacuum 24/7,
} and had not
} been allowed to warm up at all.
}
} The consumption then continued to rise, and after a further six
} months, all under
} vacuum and with no warmups, it ran out of LN2 over 3 1/2 days of
} Easter, indicating a
} consumption rate of about 2.2 litres/day.
}
} And now, after recooling, the consumtion is up to about 2.4
} litres/day, and, of course,
} the resolution has degraded by about 3 eV.
}
} It seems to me that this detector has always had a slow leak, not
} through the window,
} which the factory failed to find and fix both at the time of
} manufacture and when it was
} returned to them.
}
} The factory, however, maintains that, because it was leak-checked when
} it was
} returned to them, that any problem must have developed subsequently.
}
} Now I'm not a deeply cynical person, but it seems to me unlikely that
} twice the same
} detector has developed vacuum problems within two years of leaving the
} factory.
}
} I will be interested in the opinions of others about the history of
} this particular detector,
} and also in the experience of others with the vacuum lifetimes of
} their EDS detectors.
}
Dear Rich,
The detector we had on the HVEM also had a slow leak, and I think that
any system that has o-rings, valves, and other parts that can be
disassembled will have a slow leak. When we mounted a modified back
plate on our system to facilitate pump-outs in situ, we had to fiddle
with it to minimize the leaks, and, even so, we had to pump the system
out every few months to get good resolution. (BTW, 3 eV is very
good--I suspect you mean 300 eV.) We didn't ever have to replace the
LN2 more frequently than once a week, but your dewar may be smaller
than ours. 2+ l/d is a fast consumption rate, though. If you can hook
up a leak detector to the EDS detector, you might be able to find the
leak(s) and remedy it or them. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 20:21:31 2005



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Tue, 5 Apr 2005 11:36:20 +1000
Subject: [Microscopy] Re: [ Microscopy] EMU design lessons learned from the past

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

When we moved into our new building, a home had to be found for the
TEM. The new building, though beautiful and heritage listed sandstone,
was not exactly suited for an electron microscope. We had the choice of
the first or second floor. There was no choice of the underground
railway or the main road outside. As there was no money to pay for
proper testing, I spent a delightful day checking for vibration with a
saucer of water. I could be found for long periods at prospective
locations crouched on the floor with my saucer staring intently at the
water surface waiting for a train or the traffic lights to change. As
we in a hospital complex, this gave rise to several people solicitously
stopping and asking if I needed medical attention. I dare say they were
completely confused when I explained my mission; I really should have
thought up something more plausible, such as an alien attack!

As it turned out, the microscope is very happy and vibration free in
its second floor location!

The moral? It may seem impossible, but if there's no choice and some
imagination.......


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
On 1 Apr 2005, at 3:45 PM, Coetzee, Mr S. H Physics Science wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear All
} I just love this list. We are having a workshop on Laboratory
} infrastructure and M {management.
} Most EM Units (like ours) I know was shoved into a existing building.
} Some even of 2nd and 3rd floors.
} I will appreciate it if people can share there experience with
} relationship to performance problems observed, instrument involved (W,
} LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a
} few nice and humorous examples will be great. I hope to get the point
} across that an EM unit is a important part of a University, and if
} possible must be taken into consideration during building design and
} ultimately must play a part in the location decision of a University.
}
} Thanks
}
}
} Since some mail do get Lost, Bounces, etc Please send a duplicate/copy
} of all urgent mail to:
}
} coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
}
} Mr S. H. Coetzee
} Electron Microscope Unit
} University of Botswana
} Private Bag 0022
} Gaborone
} Botswana
} Phone : +267 355 2462/5222
} Mobile : +267 718 36547
} Fax : +267 318 5097
} e-mail : coetzees-at-mopipi.ub.bw
}



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 20:44:48 2005



From: moss-at-relia.net
Date: Mon, 4 Apr 2005 19:58:17 -0600 (MDT)
Subject: [Microscopy] Re: viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

SEM with EDX would make this a simple analysis. It could even be automated.

Bill McManus
Mt Ogden Scientific Services
moss-at-relia.net

}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (GAFTUNE-at-A0L.COM) from
} http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
} April 4, 2005 at 12:14:11
} ---------------------------------------------------------------------------
}
} Email: GAFTUNE-at-A0L.COM
} Name: GERALD FISHER
}
} Organization: NA
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: I AM AN ENGINEER WHO IS INTERESTED IN THE TECHNIQUES USED
} INIDENTIFYING PARTICLES FILTERED ONTO FILTER PAPER USING AN OPTICAL
} MICROSCOPE. THE PARTICL SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON
} NOMINAL DIAMETER OR LENGTH.
}
} THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING SOLUTIONS. THEY MIGHT
} TYPICALLY BE ACTIVATED CARBON, METAL FINES(LIKE ELEMENTAL NICKEL OR
} COPPER), FILTER MEDIA POWDERS, OR ATMOSPHERIC DIRT.
}
} ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE QUANTITY OF EACH
} PRESENT?
}
} CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?
}
} ---------------------------------------------------------------------------
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 02:03:00 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Tue, 05 Apr 2005 02:16:49 -0500
Subject: [Microscopy] Re: viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gerald,

Several thoughts:
If you are having contrast problems, try using mineral oil or immersion oil
to "remove" the paper background. Also, reflected light darkfield
microscopy often helps.

Secondly, regarding counting - if your particles are indeed only 1 micron
in size, it will probably be difficult to get enough pixels to really image
them well with a camera. I'd recommend that you try, but it might be tough
because there won't be enough pixels/particle to really limage them. If
you can, indeed, see them by eye, you may want to invest in a simple grid
that fits in your eyepiece and count the number in a representative number
of squares. If the distribution is homogeneous, you don't have to count
all the particles in all the squares. Just count a representative number
then multiply by the total number of squares.

If the particles are as small as you say they are, you might get tricky and
use a camera to project them onto a computer monitor then make a grid on a
piece of overhead transparency and count them as you see them on the monitor.

These are just several options. I'm sure the listserver will have lots of
others.

Good hunting!

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website
to learn more about "Optimizing LIght Microscopy". Copies still available
through MME... even for class-room lots ... and we give quantity discounts.


At 05:23 PM 4/4/2005, GAFTUNE-at-A0L.COM wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 04:21:16 2005



From: terry.cooper :      terry.cooper-at-btinternet.com
Date: Tue, 5 Apr 2005 10:35:45 +0100
Subject: [Microscopy] Women pioneers in EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Only picked up my e-mails after a few days but I would agree that Irene
Manton was one of the leaders at least in the UK from the 40's to the 70's.
It so happens that Dr Peter Evennett is giving a talk on the contributions
of Irene Manton (and EM in Leeds) at the 35 year Anniversary Meeting of the
SEMT (Society of Electron Microscope Technology) in The Open University,
Milton Keynes, UK on the 27th of April. If you can get there great as we
would love to see you, but if not I would guess a transcript of the talk
might be available,

Regards

Terry Cooper
TAAB Laboratories Equipment Ltd
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England

Tel ++44(0)118 981 7775
Fax ++44(0)118 981 7881
e-mail sales-at-taab.co.uk
www.taab.co.uk



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 07:59:43 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Tue, 05 Apr 2005 08:13:48 -0500
Subject: [Microscopy] Re: Re: [ Microscopy] EMU design lessons learned

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Diane,
You were very fortunate that you were able to retrofit a facility with
minimum problems to house your microscopes. This is not always possible for
high end instruments and high resolution demands.

One topic to be addressed during the Core Facility Management session at
M&M2005 is construction of a facility for these special needs. I hope all
that are fortunate enough to be able to attend the meeting will come and
share their experiences and solutions to both routine and high end
installation problems during our extensive discussion period.

I hope that the proceedings of this session will be able to be taped and
published in Microscopy Today as we have done previously so all will
benefit.

By the way, this is the first time that this session is officially
sponsored by our newly MSA-certified Focused Interest Group on Facility
Operations.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy




On 4/4/05 8:36 PM, "Diana van Driel" {dianavd-at-eye.usyd.edu.au} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} When we moved into our new building, a home had to be found for the
} TEM. The new building, though beautiful and heritage listed sandstone,
} was not exactly suited for an electron microscope. We had the choice of
} the first or second floor. There was no choice of the underground
} railway or the main road outside. As there was no money to pay for
} proper testing, I spent a delightful day checking for vibration with a
} saucer of water. I could be found for long periods at prospective
} locations crouched on the floor with my saucer staring intently at the
} water surface waiting for a train or the traffic lights to change. As
} we in a hospital complex, this gave rise to several people solicitously
} stopping and asking if I needed medical attention. I dare say they were
} completely confused when I explained my mission; I really should have
} thought up something more plausible, such as an alien attack!
}
} As it turned out, the microscope is very happy and vibration free in
} its second floor location!
}
} The moral? It may seem impossible, but if there's no choice and some
} imagination.......
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
} On 1 Apr 2005, at 3:45 PM, Coetzee, Mr S. H Physics Science wrote:
}
} }
} }
} } -----------------------------------------------------------------------
} } -------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------
} } --------
} }
} } Dear All
} } I just love this list. We are having a workshop on Laboratory
} } infrastructure and M {management.
} } Most EM Units (like ours) I know was shoved into a existing building.
} } Some even of 2nd and 3rd floors.
} } I will appreciate it if people can share there experience with
} } relationship to performance problems observed, instrument involved (W,
} } LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a
} } few nice and humorous examples will be great. I hope to get the point
} } across that an EM unit is a important part of a University, and if
} } possible must be taken into consideration during building design and
} } ultimately must play a part in the location decision of a University.
} }
} } Thanks
} }
} }
} } Since some mail do get Lost, Bounces, etc Please send a duplicate/copy
} } of all urgent mail to:
} }
} } coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
} }
} } Mr S. H. Coetzee
} } Electron Microscope Unit
} } University of Botswana
} } Private Bag 0022
} } Gaborone
} } Botswana
} } Phone : +267 355 2462/5222
} } Mobile : +267 718 36547
} } Fax : +267 318 5097
} } e-mail : coetzees-at-mopipi.ub.bw
} }
}
}




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 09:29:05 2005



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Tue, 05 Apr 2005 10:43:28 -0400
Subject: [Microscopy] Floor vibration measurements (was "EMU design lessons...")

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Diana, et al,

Concerning floor vibration.

Perhaps an easier way to use the water saucer trick is to reflect a laser
from the surface onto a wall. By having a large saucer-to-wall distance,
you can amplify the effect of the water ripples. One thing you do need to
watch out for are air currents which can also ripple the surface of the
water. I reduced this effect by taking a large cardboard box (with
appropriate cutouts) and placing it over the water dish.

I used an old HeNe laser we had in the lab, but I suspect that with a
little ingenuity, you could use a laser pointer just as well.

Does anyone else have home-made environmental sensors for EM
labs? (vibration, acoustics, EM fields, etc)?

Cheers,
Henk

At 09:36 PM 04/04/05, Diana van Driel wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all at
once. Lately it doesn't seem to be working.



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 09:37:30 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Tue, 5 Apr 2005 07:52:09 -0700 (PDT)
Subject: [Microscopy] Re: viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gerald:

I agree with the post by Bill McManus that SEM/EDS
methods are the best way to identify the contaminant
sample you described. I do this routinely for various
customers who present contaminants on filter paper. I
don't believe one micron-sized particles are amenable
to analysis using optical microscopy methods. My
approach using SEM/EDS is cutomized for each set of
analyses I perform, depending on the customer's
concerns.

I recommend you get in touch with a laboratory that
has SEM/EDS and an operator that can interpret the
results.

Stu Smalinskas, P.E.
Metallurgist
SKF USA
Plymouth, Michigan
(734) 414-6862

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Gerald Fisher wrote:

Question: I AM AN ENGINEER WHO IS INTERESTED IN THE
TECHNIQUES USED IN IDENTIFYING PARTICLES FILTERED ONTO
FILTER PAPER USING AN OPTICAL MICROSCOPE. THE PARTICLE
SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON NOMINAL
DIAMETER OR LENGTH.

THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING
SOLUTIONS. THEY MIGHT TYPICALLY BE ACTIVATED CARBON,
METAL FINES (LIKE ELEMENTAL NICKEL OR COPPER), FILTER
MEDIA POWDERS, OR ATMOSPHERIC DIRT.

ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE
QUANTITY OF EACH PRESENT?

CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?



__________________________________
Yahoo! Messenger
Show us what our next emoticon should look like. Join the fun.
http://www.advision.webevents.yahoo.com/emoticontest


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 10:30:56 2005



From: Prof. Dr. Wim Van den Broeck :      wim.vandenbroeck-at-UGent.be
Date: Tue, 5 Apr 2005 17:46:12 +0200
Subject: [Microscopy] LM embedding larvae in paraffin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

I need to embed some very small larvae of Ostertagia ostertagi in paraffin,
but they are only visible under the microscope. I have a basic protocol
advising to embed the larvae in 3% agar, fix them overnight in Bouins fluid,
and subsequently process and embed them in paraffin, but does it mean that
the larvae, pre-embedded in agar, can subsequently be fixed, processed and
embedded in paraffin ? Or do I have to remove the agar in a particular step
? Or are there other protocols that I can use ?

Thanks in advance for your comments.

Kind regards,

Wim.


---
Wim Van den Broeck, DVM, MSc, PhD
Docent Cytology and Histology
Department of Morphology,
Faculty of Veterinary Medicine, Ghent University
Salisburylaan 133, B-9820 Merelbeke
BELGIUM
tel.: +32 (0)9 264 77 16
fax: +32 (0)9 264 77 90
Email: wim.vandenbroeck-at-UGent.be
http://allserv.rug.ac.be/~bdepauw/




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 10:39:10 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Tue, 5 Apr 2005 10:54:32 -0500
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I would like to find a suitable scanner to scan Kodak 4489 film, that is:
3.25" X 4" negatives. Preferably as low a priced system as possible would
be suitable, so I'm interested to see what others have done or are doing
when it comes to scanning this size of negative.

I've already noticed that when Nikon or Minolta talks about "electron
microscope film" they have in mind a format of 59mm X 82 mm, which is too
small for us.

Your help with valuable ideas would be GREATLY appreciated in this matter.



Garry Burgess

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 11:22:29 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 05 Apr 2005 09:32:01 -0700
Subject: [Microscopy] Re: Floor vibration measurements (was "EMU design

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I use a small magnetometer that plugs into my notebook PC.
Using Spectrum Laboratory Windows app. Free download is
from here:

www . qsl. net/dl4yhf/spectra1.html

It was made by ham radio folks to recover Morse Code and
to do other radio things but is great for FFT analysis of
all sorts of vibration and acoustic noise.

gary g.
N6OIJ



At 07:43 AM 4/5/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 11:41:20 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Tue, 5 Apr 2005 11:56:42 -0500
Subject: [Microscopy] viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gerald,

There are two components to your proposed analysis: 1) you want to count
particles that are as small as 1 micrometer, and 2) you want to count
particles based on their elemental composition and/or morphology. I am
doubtful that you will be able to clearly distinguish between the groups of
particles using the light microscope and even with EDXS, you may have some
difficulty distinguishing between the activated carbon and some atmospheric
dirt and perhaps the filter media powders depending on their composition
(are they a silicon based material?); the nickel and copper would be easy to
do with EDXS as has been suggested by other replies. The other aspect of
using the light microscope would, as Ms Foster mentioned, be the ability to
image the smallest of the particles with a digital camera, too few pixels.

Another concern is the use of filter paper (depth filters) where the smaller
particles can get trapped within the matrix of the paper and be lost to
imaging on the SEM. What you need to use is a mesh type filtration
membrane. Nuclear track etched membranes can also cause "piling up" of
particles around the pore and consequently you can lose some particles
embedded in a "mountain" of other particles (I've seen this unless you have
very few particles in your solution). If you can get access to an SEM with
EDXS I have another procedure that you can use to filter the particles but
that would require that you 1) can dilute the electroplating solution with
0.45 micrometer or smaller filtered distilled water, 2) that the
electroplating solution will not attack aluminum, and 3) there is no
aluminum or gold in the solution to be filtered.

If you can live with the above limitations, dilute the electroplating
solution with filtered distilled water and filter the particles on a vacuum
evaporated gold coated 0.2 micrometer retention rated Anodisc(TM) filtration
membrane. This filtration medium is like a filter screen so the particles
will all sit atop the flat surface. I've found that coating helps reduce
clumping of the particles and of course reduces charging. The total
particulate burden can be easily determined using a series of Fovea Pro 3.0
(plug-ins for Photoshop) steps which I can give you (other software can
probably do the same thing). The EDXS with other dedicated image analysis
software can also do this for each type of particle based on elemental
composition (probably even automated) although again, separating some of the
types of particles may be problematic.

Damian Neuberger, Ph.D.
2416 Covert Rd
Glenview IL 60025
Tel: (847) 998-8574
email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}


-----Original Message-----
} From: by way of MicroscopyListserver [mailto:GAFTUNE-at-A0L.COM]
Sent: Monday, April 04, 2005 5:24 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (GAFTUNE-at-A0L.COM) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April
4, 2005 at 12:14:11
---------------------------------------------------------------------------

Email: GAFTUNE-at-A0L.COM
Name: GERALD FISHER

Organization: NA

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I AM AN ENGINEER WHO IS INTERESTED IN THE TECHNIQUES USED
INIDENTIFYING PARTICLES FILTERED ONTO FILTER PAPER USING AN OPTICAL
MICROSCOPE. THE PARTICL SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON
NOMINAL DIAMETER OR LENGTH.

THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING SOLUTIONS. THEY MIGHT
TYPICALLY BE ACTIVATED CARBON, METAL FINES(LIKE ELEMENTAL NICKEL OR COPPER),
FILTER MEDIA POWDERS, OR ATMOSPHERIC DIRT.

ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE QUANTITY OF EACH
PRESENT?

CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?

---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:06:22 2005



From: Tom Murray :      murraytm-at-u.washington.edu
Date: Tue, 5 Apr 2005 10:21:26 -0700
Subject: [Microscopy] Digital Camera Recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am looking at moving my Philips EM420 from film to digital imaging.
This is a materials science program so diffraction as well as
bright-field imaging are important.

I have done an online search for suppliers for digital cameras and
found the following:

Gatan
SIS
Teitz
SIA

As with everyone money is a factor in this decision.

I am looking for recommendations in two areas.

1) Do you have experience with cameras from the above manufacturers, or
other manufacturers my search missed? Are you happy with the product
and the support?

2) What are the pros and cons of top mount vs bottom mount cameras?
I know the top mount cameras have imaging areas which are
similar to the plate film my students are used to, but there is lower
resolution. Any other tradeoffs to consider?

Thanks for your help,

Tom

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email: murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:19:50 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 5 Apr 2005 13:36:58 -0400
Subject: [Microscopy] Re: NESM 22nd Annual Spring Symposium-Woods Hole, MA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The New England Society for Microscopy (NESM), along with it's co-host, the
Connecticut Microscopy Society (CMS)is pleased to announce it's 22nd Annual
Spring Symposium at Marine Biological Lab (MBL) in Woods Hole, MA. The 2-day
Symposium will be held Friday April 29th and Saturday, April 30th. Preceeding
the symposium on Thursday, April 28th, there will be a half-day workshop on
"Presentation Skills and Details for Microscopists" presented by Paul Matsudaira
of The Whitehead Institute-MIT (Cambridge, MA).

The program will include presentations from both the Materials Science and
Biological Sciences. After registration at Noon on Friday, and the welcome by
NESM's President-elect, Dan Gibson, there will be 2 technical sessions which
will include such fascinating talks as large X-ray mapping for the analysis of
geological samples and the use of Northeastern's Keck 3D fusion microscope to
examine protein expression in mouse embryos. At the close of the technical
presentations on Friday, attendees will enjoy socializing at a cocktail hour and
dinner at MBL's Swope Center with a wonderful view of Eel Pond. The
after-dinner speaker, Dr. Greg erickson of Florida Statue University, will
present an interesting talk entitled, "Building Giants: Unlocking the Mysteries
of Dinosaurian Growth Patterns".

The Saturday morning session will include 3 talks, after which the attendees can
meet with our sponsors in the vendor display and peruse the Poster session (many
submitted by students). Monetary prizes will be awarded for the Best Poster,
Best Student Poster, and Best Photo-as-Art. Do come and support our poster
entrants for all their hard work!

Pre-registration is mandatory: if you plan on staying for dinner and if you want
to attend the workshop, you must reserve your spot. Complete details re: the
meeting program, the Poster Session, and the workshop (which is a separate
registration from the Symposium) can be found on NESM's website
(http://nesm.cims.harvard.edu) under "current newsletter".

If you require any information re: the meeting, please contact Paul Bain, NESM's
Treasurer at paul_bain-at-HMS.HARVARD.EDU or by phone: 617-432-3236.

Please plan on joining us at Woods Hole! It is one of the most anticipated
meetings of the year for NESM.

Peggy Sherwood
Corresponding Secretary/Newsletter Editor-NESM











Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:31:19 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Tue, 05 Apr 2005 12:46:16 -0500
Subject: [Microscopy] Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

a key feature in the selection of a scanner for EM film is the Dmax. most
"home" flat bed scanners don't have a sufficiently high enough Dmax value
to capture the essential information. my epson 1680 does a decent job.


} X-Authentication-Warning: ns.microscopy.com: mail set sender to
} MicroscopyL-request-at-ns.microscopy.com using -f
} Date: Tue, 5 Apr 2005 10:54:32 -0500
} From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
} Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives
} To: {microscopy-at-microscopy.com}
} X-Mailer: Internet Mail Service (5.5.2653.19)
} X-OriginalArrivalTime: 05 Apr 2005 17:28:06.0738 (UTC)
} FILETIME=[D461B320:01C53A04]
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:49:36 2005



From: ekomarnicki-at-MacDermid.com
Date: Tue, 5 Apr 2005 14:05:25 -0400
Subject: [Microscopy] Surface roughness using SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Does any on the list know if one can perform surface roughness
measurements using a SEM either directly or through EDS software? I am
aware that this can be accomplished using AFMs and light and laser
profilometers, but is there software out there that allows the use of a
SEM?

TIA,

Ed Komarnicki
Sr. Analytical Chemist
MacDermid Inc.



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:53:06 2005



From: Yang, Ann-Fook :      YANGA-at-AGR.GC.CA
Date: Tue, 5 Apr 2005 14:03:28 -0400
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



I am quite happy with Epson Perfection 4870 Photo flatbed scanner (just a happy user).

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Tuesday, April 05, 2005 11:55 AM
To: microscopy-at-microscopy.com


I would like to find a suitable scanner to scan Kodak 4489 film, that is:
3.25" X 4" negatives. Preferably as low a priced system as possible would
be suitable, so I'm interested to see what others have done or are doing
when it comes to scanning this size of negative.

I've already noticed that when Nikon or Minolta talks about "electron
microscope film" they have in mind a format of 59mm X 82 mm, which is too
small for us.

Your help with valuable ideas would be GREATLY appreciated in this matter.



Garry Burgess

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 13:14:08 2005



From: Richard Edelmann :      edelmare-at-MUOhio.Edu
Date: Tue, 5 Apr 2005 13:59:30 -0400
Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Folks:

Help! I need a different name to call "Dualbeam" or "Crossbeam"
instruments since one is trademarked and the other is registered (FEI &
Zeiss).



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 13:26:51 2005



From: Montpetit, Diane :      MontpetitD-at-agr.gc.ca
Date: Tue, 5 Apr 2005 14:40:12 -0400
Subject: [Microscopy] bacteria capsular material and lectin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,



I want to stabilize the capsule surrounding a bacteria in order to see if the lectin I intend to use is binding to a sugar residue located in the capsule itself or on the cell wall.



I have already tried negative staining (PTA) and I was not satisfy with the results. There is some binding but I cannot discriminate clearly if it on the capsule or the wall.



I decided to go for thin sections but I need to stabilize the capsule, with ruthenium red per exemple, in order to avoid its collapse, but I am wondering if the affinity lectin/sugar residue will be affected by the stabilization.



Any ideas anyone?


Diane Montpetit
Centre de Recherche et de Développement sur les Aliments
3600 Boul. Casavant Ouest,
St-Hyacinthe, (Québec) Canada J2S 8E3
Téléphone/Phone: 450-773-1105
Télécopieur/ Fax: 450-773-8461
montpetitd-at-agr.gc.ca




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 13:31:29 2005



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Tue, 5 Apr 2005 15:43:58 -0400
Subject: [Microscopy] Scaning 3 1/4x 4 negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
We use Microtek ScanMaker 6800 with acceptable results.
Dorota


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 14:12:04 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 5 Apr 2005 15:28:05 -0400
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We have the Epson Perfection 3200 scanner and like it very much.

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Tuesday, April 05, 2005 11:55 AM
To: microscopy-at-microscopy.com


I would like to find a suitable scanner to scan Kodak 4489 film, that is:
3.25" X 4" negatives. Preferably as low a priced system as possible would
be suitable, so I'm interested to see what others have done or are doing
when it comes to scanning this size of negative.

I've already noticed that when Nikon or Minolta talks about "electron
microscope film" they have in mind a format of 59mm X 82 mm, which is too
small for us.

Your help with valuable ideas would be GREATLY appreciated in this matter.



Garry Burgess

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg Canada

This e-mail and/or any documents in this transmission is intended for the
address(s) only and may contain legally privileged or confidential information.
Any unauthorized use, disclosure, distribution, copying or dissemination is
strictly prohibited. If you receive this transmission in error, please notify
the sender immediately and return the original.




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 14:12:21 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 05 Apr 2005 15:26:41 -0400
Subject: [Microscopy] Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
I have an Epson Expression 1600 (its about 4 years old) that works
well for me. It has 1600 dpi resolution It does not have a holder
that fits the negatives, but it does have 2 focus settings (one for
film in holders and a 0mm distance for "contact prints"). As long as
my film is perfectly dry and I position it carefully, I have no
problems with Newton rings. I do scan it as positive film and then
reverse contrast in Photoshop, since the settings for scanning
negative film give really awful results.
When new, the scanner cost around $2,500, which at the time was half
of my budget for a new computer system. I don't what what the
current prices are, but I think that I get a lot of bang for my buck.
Just a happy user....
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 14:31:50 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Tue, 5 Apr 2005 15:44:20 -0400
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Richard,
You might try "Orthobeam" (though perhaps "Onthebeam" would
be more poetic?) or "Pairabeam".

TB


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 15:32:04 2005



From: Hicks, Aaron :      A.W.Hicks-at-massey.ac.nz
Date: Wed, 6 Apr 2005 08:47:35 +1200
Subject: [Microscopy] Re: Floor vibration measurements (was "EMU design

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Do you have a link for the magnetometer you use?

Aaron Hicks
Electron Microscopy Preparation Technician

Comparative Physiology and Anatomy
Institute of Veterinary, Animal, and Biomedical Sciences
Massey University

PN-412
Private Bag 11 222
Palmerston North
New Zealand

Phone +64 06 350 4874


-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Wednesday, 6 April 2005 4:32 a.m.
To: Hendrik O. Colijn
Cc: MSA listserver

I use a small magnetometer that plugs into my notebook PC. Using
Spectrum Laboratory Windows app. Free download is from here:

www . qsl. net/dl4yhf/spectra1.html

It was made by ham radio folks to recover Morse Code and
to do other radio things but is great for FFT analysis of
all sorts of vibration and acoustic noise.

gary g.
N6OIJ



At 07:43 AM 4/5/2005, you wrote:


} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} water. I reduced this effect by taking a large cardboard box (with
} appropriate cutouts) and placing it over the water dish.
}
} I used an old HeNe laser we had in the lab, but I suspect that with a
} little ingenuity, you could use a laser pointer just as well.
}
} Does anyone else have home-made environmental sensors for EM
} labs? (vibration, acoustics, EM fields, etc)?
}
} Cheers,
} Henk
}
} At 09:36 PM 04/04/05, Diana van Driel wrote:
}
}
} } ----------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} } was not exactly suited for an electron microscope. We had the choice
} } of the first or second floor. There was no choice of the underground
} } railway or the main road outside. As there was no money to pay for
} } proper testing, I spent a delightful day checking for vibration with a

} } saucer of water. I could be found for long periods at prospective
} } locations crouched on the floor with my saucer staring intently at the

} } water surface waiting for a train or the traffic lights to change. As
} } we in a hospital complex, this gave rise to several people
} } solicitously stopping and asking if I needed medical attention. I dare

} } say they were completely confused when I explained my mission; I
} } really should have thought up something more plausible, such as an
} } alien attack!
} }
} } As it turned out, the microscope is very happy and vibration free in
} } its second floor location!
} }
} } The moral? It may seem impossible, but if there's no choice and some
} } imagination.......
} }
} }
} } Diana van Driel
} } Dept Ophthalmology
} } Sydney University
} } GPO Box 4337
} } Sydney, NSW
} } AUSTRALIA 2001
} }
} } Phone 61 2 93827278
} } Mobile 0423 151614
} } FAX 61 2 93827318
} } On 1 Apr 2005, at 3:45 PM, Coetzee, Mr S. H Physics Science wrote:
} }
} } }
} } }
} } } ---------------------------------------------------------------------
} } } --
} } } -------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America

} } } shoved into a existing building. Some even of 2nd and 3rd floors.
} } } I will appreciate it if people can share there experience with
} } } relationship to performance problems observed, instrument involved
(W,
} } } LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a
} } } few nice and humorous examples will be great. I hope to get the
point
} } } across that an EM unit is a important part of a University, and if
} } } possible must be taken into consideration during building design and
} } } ultimately must play a part in the location decision of a University.
} } }
} } } Thanks
} } }
} } }
} } } Since some mail do get Lost, Bounces, etc Please send a
} } } duplicate/copy of all urgent mail to:
} } }
} } } coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
} } }
} } } Mr S. H. Coetzee
} } } Electron Microscope Unit
} } } University of Botswana
} } } Private Bag 0022
} } } Gaborone
} } } Botswana
} } } Phone : +267 355 2462/5222
} } } Mobile : +267 718 36547
} } } Fax : +267 318 5097
} } } e-mail : coetzees-at-mopipi.ub.bw
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://www.ceof.ohio-state.edu
} Time is that quality of nature which keeps events from happening all at
} once. Lately it doesn't seem to be working.





From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 15:48:37 2005



From: Ian Hallett :      IHallett-at-hortresearch.co.nz
Date: Wed, 06 Apr 2005 09:04:58 +1200
Subject: [Microscopy] Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Garry

I would second Ann Fook Yang's comments on the Epson 4870, it has a quoted DMax of 3.8 and certainly compares well with a dedicated negative scanner used by our photographic section. Like most scanners it does not have a negative holder that exactly fits the 4489 size but the films can be securely held by the 4" x 5" holder.

Ian



Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz


} } } Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6/04/2005 3:54:32 a.m. } } }


------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I would like to find a suitable scanner to scan Kodak 4489 film, that is:
3.25" X 4" negatives. Preferably as low a priced system as possible would
be suitable, so I'm interested to see what others have done or are doing
when it comes to scanning this size of negative.

I've already noticed that when Nikon or Minolta talks about "electron
microscope film" they have in mind a format of 59mm X 82 mm, which is too
small for us.

Your help with valuable ideas would be GREATLY appreciated in this matter.



Garry Burgess

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 15:54:07 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Tue, 5 Apr 2005 15:08:24 -0600
Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Richard,

how about:

Double-beam
2-beam
Two-beam
FIB/SEM
SEM/FIB

Btw, I don't know if by making these suggestions here I would infringe or
perhaps gain copyright to these words. In any case, I don't mind if you use
any of them ;-)

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.Edu]
Sent: Tuesday, April 05, 2005 12:00
To: microscopy-at-MSA.Microscopy.com

Folks:

Help! I need a different name to call "Dualbeam" or "Crossbeam"
instruments since one is trademarked and the other is registered (FEI &
Zeiss).



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 16:00:18 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Tue, 5 Apr 2005 15:14:10 -0600
Subject: [Microscopy] Surface roughness using SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Ed,

depending on the amplitude of the roughness, you could use Stereo imaging
and analysis. Essentially, you take a stereo pair and run it through
software to obtain a surface profile. We can do this with our stereo package
for the Scandium software, contact me off-line if you need further
information. The surface profile can then be analyzed to get roughness
parameters (r_sub_m, r_sub_q, etc.).

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: ekomarnicki-at-MacDermid.com [mailto:ekomarnicki-at-MacDermid.com]
Sent: Tuesday, April 05, 2005 12:05
To: microscopy-at-MSA.microscopy.com

Does any on the list know if one can perform surface roughness
measurements using a SEM either directly or through EDS software? I am
aware that this can be accomplished using AFMs and light and laser
profilometers, but is there software out there that allows the use of a
SEM?

TIA,

Ed Komarnicki
Sr. Analytical Chemist
MacDermid Inc.



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 16:58:29 2005



From: Tom Murray :      murraytm-at-u.washington.edu
Date: Tue, 5 Apr 2005 15:13:46 -0700
Subject: [Microscopy] Re: Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

You could go with multi-beam. Even though it is only two it is
technically multi.


On Apr 5, 2005, at 12:44 PM, Tobias Baskin wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Richard,
} You might try "Orthobeam" (though perhaps "Onthebeam" would be more
} poetic?) or "Pairabeam".
}
} TB
}
}
} } ----------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ---------
} }
} } Folks:
} }
} } Help! I need a different name to call "Dualbeam" or "Crossbeam"
} } instruments since one is trademarked and the other is registered (FEI
} } &
} } Zeiss).
} }
} }
} }
} } Richard E. Edelmann, Ph.D.
} } Associate Editor of EXPO, Microscopy and Microanalysis Supplement
} } Electron Microscopy Facility Director
} } 350 Pearson Hall
} } Miami University, Oxford, OH 45056
} } Ph: 513.529.5712 Fax: 513.529.4243
} } E-mail: edelmare-at-muohio.edu
} } http://www.emf.muohio.edu
}
}
} --
} _ ____ __ ____ / \ / / \ /
} \ \ Tobias I. Baskin
} / / / / \ \ \ Biology Department
} /_ / __ /__ \ \ \__ 611 N. Pleasant St.
} / / / \ \ \ University of
} Massachusetts
} / / / \ \ \ Amherst, MA, 01003
} / / ___ / \ \__/ \ ____
} http://www.bio.umass.edu/biology/baskin/
} Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
}
}

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email: murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 17:30:38 2005



From: hkonishi-at-indiana.edu
Date: Tue, 5 Apr 2005 17:46:18 -0500
Subject: [Microscopy] Si and O in lacey/holey C film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I am wondering why Si and O are detected from commercial C films (two
companies). I measured C film and empty area (hole) next to the film, and I
only detected Si and O from C film. I think that Si and O really come from C
film.

I think that C was coated on plastic film with small holes. Commercial
lacey/holey C films are pure C or C on plastic. I don't think that plastic
contains Si.

I would appreciate any suggestion you might have.

Thank you,
Hiromi Konishi
IU and JHU


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 17:35:56 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 5 Apr 2005 16:07:02 -0700
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 5, 2005, at 10:59 AM, Richard Edelmann wrote:

} Help! I need a different name to call "Dualbeam" or "Crossbeam"
} instruments since one is trademarked and the other is registered (FEI &
} Zeiss).
}
Dear Richard,
How about "colliding beam"? I'm sure that the accelerator physics
people let that one get into the public domain.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 17:38:54 2005



From: George Theodossiou :      George.Theodossiou-at-amcor.com.au
Date: Wed, 6 Apr 2005 08:53:59 +1000
Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

BiBeam, DuoBeam, DeauxBeam, DiploBeam, MultiBeam......

-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.Edu]
Sent: Wednesday, 6 April 2005 04:00
To: microscopy-at-MSA.Microscopy.com

Folks:

Help! I need a different name to call "Dualbeam" or "Crossbeam"
instruments since one is trademarked and the other is registered (FEI &
Zeiss).



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


************************************************************************
CAUTION - This message may contain privileged and confidential
information intended only for the use of the addressee named above.
If you are not the intended recipient of this message you are hereby
notified that any use, dissemination, distribution or reproduction of
this message is prohibited. If you have received this message in error
please notify AMCOR immediately.
Any views expressed in this message are those of the individual sender
and may not necessarily reflect the views of AMCOR.
************************************************************************



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 18:28:44 2005



From: Ivan Petrov :      petrov-at-mrl.uiuc.edu
Date: Tue, 5 Apr 2005 18:44:45 -0500
Subject: [Microscopy] Job opening at CMM/UIUC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

FYI, this announcement is posted on the MSA web-site.

Regards

Ivan



University of Illinois at Urbana-Champaign

Frederick Seitz Materials Research Laboratory

SENIOR RESEARCH SCIENTIST IN ELECTRON MICROSCOPY

The Frederick Seitz Materials Research Laboratory at the University of
Illinois is seeking an experienced electron microscopist as a staff
member in the Center for Microanalysis of Materials. The Center is a
major DOE sponsored research facility for electron beam
microcharacterization and maintains state of the art electron microscopy
instrumentation as well as instruments for surface microanalysis, x-ray
diffraction and other analytical techniques.

The successful candidate will focus mainly on analytical STEM/TEM but is
expected to have the experience and the flexibility to work on other
techniques if needed. We are particularly interested in hiring a person
with experience in working with field emission TEMs, EDS and EELS
techniques. The Center houses five TEMs including a VG HB 501, JEOL
2010F, and is expected soon to acquire a Cs-corrected STEM instrument
with a monochromator and in-colunm energy filter. The person appointed
will have primary responsibility for these advanced instruments. The
successful candidate will also be responsible for facilitating the
research of approximately 50 users per year on advanced TEM and STEM
techniques. With the broad range of research programs active at the
Center, the position will offer ample opportunity to develop an
interactive research program as well as to pursue individual research
agenda.

This position requires a Ph.D. in Physics, Chemistry, Materials Science,
or related discipline with at least three years experience in advanced
electron microscopy techniques. Salary is commensurate with experience
and qualifications.

This is a full-time appointment with standard university benefits. The
proposed starting date is as soon as possible after the closing date.
In order to ensure full consideration, applications must be received by
May 15, 2005. Please send letter of application, resume, and names and
addresses of three references to Ivan Petrov, c/o Susan Logan,
University of Illinois, Materials Research Laboratory, 104 South Goodwin
Avenue, Urbana, Illinois 61801, phone (217) 244-2944.

The University of Illinois is an Affirmative Action/Equal Opportunity
Employer.


=====================================================================
Prof. Ivan Petrov, Director
Center for Microanalysis of Materials http://cmm.mrl.uiuc.edu
Frederick Seitz Materials Research Laboratory
University of Illinois tel: 217-333-8396
104 S. Goodwin Avenue fax: 217-244-2278
Urbana, IL 61801 USA email: petrov-at-uiuc.edu
=====================================================================



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 18:31:21 2005



From: Ronald Anderson :      randerson20-at-tampabay.rr.com
Date: Tue, 05 Apr 2005 19:46:28 -0400
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Substitute "column" for "beam" in any of the suggestions. Dual-Column
and Cross-Column seem to work.

Ron Anderson


Richard Edelmann wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 18:56:51 2005



From: :      lgiannuzzi-at-adelphia.net
Date: Tue, 5 Apr 2005 20:11:27 -0400
Subject: [Microscopy] Re: RE: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

To avoid any such problems, we have referred to these instruments in the M&M "FIB" session as:

"Dual Platform" Instruments or how about

"Dual Column" Instruments or

"Multi-Column Platforms"

Lucille Giannuzzi
FEI Company


---- George Theodossiou {George.Theodossiou-at-amcor.com.au} wrote:
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} BiBeam, DuoBeam, DeauxBeam, DiploBeam, MultiBeam......
}
} -----Original Message-----
} } From: Richard Edelmann [mailto:edelmare-at-MUOhio.Edu]
} Sent: Wednesday, 6 April 2005 04:00
} To: microscopy-at-MSA.Microscopy.com
} Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name
}
}
}
} ----------------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America To



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 19:19:02 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 5 Apr 2005 17:50:08 -0700
Subject: [Microscopy] Re: Si and O in lacey/holey C film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 5, 2005, at 3:46 PM, hkonishi-at-indiana.edu wrote:

} I am wondering why Si and O are detected from commercial C films (two
} companies). I measured C film and empty area (hole) next to the film,
} and I
} only detected Si and O from C film. I think that Si and O really come
} from C
} film.
}
} I think that C was coated on plastic film with small holes. Commercial
} lacey/holey C films are pure C or C on plastic. I don't think that
} plastic
} contains Si.
}
} I would appreciate any suggestion you might have.

Dear Hiromi,
If the films are prepared by coating the plastic on a glass slide,
evaporating C onto the plastic, placing the coating onto grids (or
grids onto coating), then dissolving away the plastic, some of the Si
(and maybe O) could be removed from the glass and remain on the C even
after the plastic is dissolved, since the Si may be insoluble in the
organic solvent used to remove the plastic. If, however, the plastic
is cast onto water, picked up onto grids and C evaporated onto it
without using glass in any part of the process (except as a major
constituent of the bell jar in the evaporator), I would not expect to
see Si, although O is still possible. You could prepare films yourself
using each method and see if my expectations are borne out. I would be
interested in what the vendors who sell C-coated grids have to say.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 19:22:15 2005



From: somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Tue, 5 Apr 2005 19:38:12 -0500
Subject: [Microscopy] viaWWW: thanks Dr Rosemary white

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 4, 2005 at 23:12:44
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh

Organization: urmia university

Title-Subject: [Microscopy] [Filtered] thanks Dr Rosemary white

Question: Dear Dr White

Thanks for replying me for the lenghth of FAA preserved material.
SO if the FAA shrinks the material what can I do with fruit
preserved in FAA?can I use these material or they have become unconsumable and useless?
and should I provide some other samples in a new FAA solution?


may this shrinking happen if I provide sectioning by microtomy or not?

thank you very much

Somayyeh

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 19:22:50 2005



From: guillet-at-chim.ucl.ac.be (by way of MicroscopyListserver)
Date: Tue, 5 Apr 2005 19:38:34 -0500
Subject: [Microscopy] viaWWW: osmium selective staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (guillet-at-chim.ucl.ac.be) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, April 5, 2005 at 12:33:44
---------------------------------------------------------------------------

Email: guillet-at-chim.ucl.ac.be
Name: Pierre

Title-Subject: [Microscopy] [Filtered] MListserver: osmium selective staining?

Question: Hi,
I'm looking for to choose a selective staining for polystyrene against polyethyleneoxyde. Before, I've tried ruthenium tetraoxide, but unsuccesfully.
I've plenty of hopes with osmium tetraoxide, but I don't know precisely its staining mecanism: do you think that polyethyleneoxide will be preserved from OsO4? Have you some tips about it?
Thank you.

Pierre GUILLET
PhD Student
University of Louvain-La-Neuve
Belgium

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 19:22:51 2005



From: montpetitd-at-agr.gc.ca (by way of MicroscopyListserver)
Date: Tue, 5 Apr 2005 19:38:54 -0500
Subject: [Microscopy] viaWWW: bacteria capsular material and lectins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (montpetitd-at-agr.gc.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, April 5, 2005 at 12:34:18
---------------------------------------------------------------------------

Email: montpetitd-at-agr.gc.ca
Name: diane montpetit

Organization: agriculture canada

Title-Subject: [Microscopy] [Filtered] bacteria capsular material and lectins

Question: Hello all,

I need to stabilize the capsular material surrounding a bacteria, in order to see if the lectin I intend to use is directed againts a sugar residue standing on the capsular material or on the cell wall itself.

I have already looked at the bacteria with PTA (negative staining)and I am not satisfied. There is binding but it is still unclear, so I decided that I would go for thin sectioning.

But I believe I need to somehow stabilize the capsule, in order to avoid most of the collapsing, and I am wondering if doing so, using ruthenium red per exemple, I would affect the affinity lectin/sugar ?

Anyone has an idea?

thank you,
Diane

Diane Montpetit
Centre de Recherche et de DÈveloppement sur les Aliments
3600 Boul. Casavant Ouest,
St-Hyacinthe, (QuÈbec) Canada J2S 8E3
TÈlÈphone/Phone: 450-773-1105
TÈlÈcopieur/ Fax: 450-773-8461
montpetitd-at-agr.gc.ca




---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 22:31:23 2005



From: Luc Harmsen :      luc-at-anaspec.co.za
Date: Wed, 6 Apr 2005 05:46:11 +0200
Subject: [Microscopy] Re: [ Microscopy] EMU design lessons learned from the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

As can be seen by the responses there are quite a variety of methods to
check a lab.
Unfortunately we are always required to do it the correct way and use the
expensive test kits.
In 90% of cases where we do a pre-install site check there is never a
problem for the applications of 80% of our customers.

I will define that. We install SEMs on Mine sites where at 12pm every day
they blast with dynamite. It's no problem. Its not that they get all the lab
staff to lift the SEM off the floor for that 1/2 a second. The SEM is
actually running at the time. They use the SEM to analyze rock particle
sections on a 24-7 basis. So, yes when the blast occurs possibly 1 or 2
particle sections may have a bit of a jaggered edge to them but in a total
of possibly 2000 sections in that run, who cares.

I would also dare to say for most SEM operators who never go over 10,000
times are even aware that their SEM may have vibration from old fans etc.

Vibration and fields only really start becoming a problem when you are
expecting to really drive the EM. FEG's and TEM's being used for
micrographing Diffraction patterns need to have a decent vibration and field
check done.

So my point here could really be that in a perfect world we would all pay
the service company to do a thorough job on a site inspection, but on realty
university budgets, in most cases the cup of water and working credit cards
after them being in a room can also work. It mostly depends on what you are
going to expect from the system.

Then the second consideration is; how easy can we move the unit, if that
method did not work. Most modern SEM's come on wheels. But try de-installing
a TEM if you find fields in the room you thought was OK. A bit more costly
than possibly having paid for the site check and sharing a coffee with you
friendly support engineer before the install. It's all about risk and who
gets blamed when it does not work.

Luc Harmsen
www.Anaspec.co.za


-----Original Message-----
} From: Diana van Driel [mailto:dianavd-at-eye.usyd.edu.au]
Sent: 05 April 2005 03:36
To: Coetzee, Mr S. H Physics Science
Cc: Microscopy

When we moved into our new building, a home had to be found for the
TEM. The new building, though beautiful and heritage listed sandstone,
was not exactly suited for an electron microscope. We had the choice of
the first or second floor. There was no choice of the underground
railway or the main road outside. As there was no money to pay for
proper testing, I spent a delightful day checking for vibration with a
saucer of water. I could be found for long periods at prospective
locations crouched on the floor with my saucer staring intently at the
water surface waiting for a train or the traffic lights to change. As
we in a hospital complex, this gave rise to several people solicitously
stopping and asking if I needed medical attention. I dare say they were
completely confused when I explained my mission; I really should have
thought up something more plausible, such as an alien attack!

As it turned out, the microscope is very happy and vibration free in
its second floor location!

The moral? It may seem impossible, but if there's no choice and some
imagination.......


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
On 1 Apr 2005, at 3:45 PM, Coetzee, Mr S. H Physics Science wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear All
} I just love this list. We are having a workshop on Laboratory
} infrastructure and M {management.
} Most EM Units (like ours) I know was shoved into a existing building.
} Some even of 2nd and 3rd floors.
} I will appreciate it if people can share there experience with
} relationship to performance problems observed, instrument involved (W,
} LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a
} few nice and humorous examples will be great. I hope to get the point
} across that an EM unit is a important part of a University, and if
} possible must be taken into consideration during building design and
} ultimately must play a part in the location decision of a University.
}
} Thanks
}
}
} Since some mail do get Lost, Bounces, etc Please send a duplicate/copy
} of all urgent mail to:
}
} coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
}
} Mr S. H. Coetzee
} Electron Microscope Unit
} University of Botswana
} Private Bag 0022
} Gaborone
} Botswana
} Phone : +267 355 2462/5222
} Mobile : +267 718 36547
} Fax : +267 318 5097
} e-mail : coetzees-at-mopipi.ub.bw
}





From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 23:58:37 2005



From: Ian Hallett :      IHallett-at-hortresearch.co.nz
Date: Wed, 06 Apr 2005 17:15:53 +1200
Subject: [Microscopy] Freeze Substitution Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have any views on the relative merits of the RMC Boeckler FS-7500 and Leica EM AFS freeze substitution systems.

Thanks

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 01:25:23 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 5 Apr 2005 23:40:23 -0700
Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Richard;
I don't think you can trademark the English language. Dual and
Beam with a space between them can be used as a descriptor as long as
you use something else to be your tool's model name, and the tool itself
does not violate patents.

John Mardinly
408-765-2346
877-277-1182


-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.Edu]
Sent: Tuesday, April 05, 2005 11:00 AM
To: microscopy-at-MSA.Microscopy.com

Folks:

Help! I need a different name to call "Dualbeam" or "Crossbeam"

instruments since one is trademarked and the other is registered (FEI &
Zeiss).



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 03:54:23 2005



From: gillian.2.brown-at-gsk.com
Date: Wed, 6 Apr 2005 10:09:20 +0100
Subject: [Microscopy] Assessing and quantitation of mast cell degranulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I have posted a question about guinea-pig mast cells and their
demonstration (many thanks to Karen Bentley for the most successful
method). However we have moved on and we now need to actually catch
degranulation in progress. Due to the difficulty of guinea-pig we are
moving to the mouse. Other getting tissue quickly post the 'trigger' then
glut fixing, osmium and processing to resin and looking at the Tol blue
for purple/pink granules a la Dvorak, Blood 1994 83:3600-3612 and Oliani
2001 Cell Biol Int 25:795-803, does any-one know of an other method?
We are doing this in the nasal airways and will be taking lavages etc so
can measure tryptase histamine but these are considered circumstantial.
The tryptase antibody we use for human does not work in mouse but has
any-one any ideas for 'activated' mast cell markers tinctorial or IHC I
would be most grateful.

many thanks
Gillian Brown


Histopathology Group
GlaxoSmithKline Medicines Research Centre,





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 07:13:47 2005



From: AtcSEM :      atcsem-at-sbcglobal.net
Date: Wed, 6 Apr 2005 08:29:24 -0400
Subject: [Microscopy] RE: Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

How about "DuoBeam"?

Pavel Lozovyy
ATC SEM Lab
(216)692-6637
http://www.atclabs.com/SEM.htm
 
 
 
 


| -----Original Message-----
| From: Tobias Baskin [mailto:baskin-at-bio.umass.edu]
| Sent: Tuesday, April 05, 2005 3:44 PM
| To: edelmare-at-MUOhio.Edu
| Cc: microscopy-at-MSA.Microscopy.com
| Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name
|
|
|
| --------------------------------------------------------------------------
| ----
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe --
| http://www.microscopy.com/MicroscopyListserver
| On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
| --------------------------------------------------------------------------
| -----
|
| Richard,
| You might try "Orthobeam" (though perhaps "Onthebeam" would
| be more poetic?) or "Pairabeam".
|
| TB
|
|
| } -------------------------------------------------------------------------
| -----
| } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| } To Subscribe/Unsubscribe --
| http://www.microscopy.com/MicroscopyListserver
| } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
| } -------------------------------------------------------------------------
| ------
| }
| } Folks:
| }
| } Help! I need a different name to call "Dualbeam" or "Crossbeam"
| } instruments since one is trademarked and the other is registered (FEI &
| } Zeiss).
| }
| }
| }
| } Richard E. Edelmann, Ph.D.
| } Associate Editor of EXPO, Microscopy and Microanalysis Supplement
| } Electron Microscopy Facility Director
| } 350 Pearson Hall
| } Miami University, Oxford, OH 45056
| } Ph: 513.529.5712 Fax: 513.529.4243
| } E-mail: edelmare-at-muohio.edu
| } http://www.emf.muohio.edu
|
|
| --
| _ ____ __ ____
| / \ / / \ / \ \ Tobias I. Baskin
| / / / / \ \ \ Biology Department
| /_ / __ /__ \ \ \__ 611 N. Pleasant St.
| / / / \ \ \ University of
| Massachusetts
| / / / \ \ \ Amherst, MA, 01003
| / / ___ / \ \__/ \ ____
| http://www.bio.umass.edu/biology/baskin/
| Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 08:13:55 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 6 Apr 2005 08:28:51 -0500
Subject: [Microscopy] Re: Sputter Coating Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

After receiving all the very helpful replies on this topic, we decided
to severely limit the use of our machine for "industrial strength" uses.
Unfortunately I was about a week too late, and it looks like a board
fried, probably, I'm told, due to being run too hard for too long.
Except for an old conventionally pumped Au coater, we are down.....

So, a word to the wise out on the list----unless you have a } 15 year old
Emscope SC500, best keep your general use coaters for general use and
leave the heavy duty stuff to the big machines. And, David, can we
borrow your coater for a couple weeks?

Sputterlessly,
Randy

-----Original Message-----
} From: David Patton [mailto:David.Patton-at-uwe.ac.uk]
Sent: Wednesday, April 06, 2005 7:13 AM
To: Tindall, Randy D.

Hi All,

Many thanks for the replies to my original question on the scaling Pt
target and microcracks in the specimen coating. The consensus seems to
be that overheating is the problem.

I wasn't clear in my original posting about why we are doing this.
These specimens are not being coated for SEM viewing, but because we
have the only sputter coater on campus and the particular lab doing all
this work requires an extremely heavy layer of whatever metal they are
using---which may be Ni, Pt, or Ta. They are the ones driving the 90 mA
and repeated 4 minute cycles (because 4 minutes is the longest time we
can program into the coater and there is no manual on/off, if we want a
16 minute coat, we have to do 4 runs). This is very heavy use for a
coater designed for general EM use, rather than industrial strength use,
and I am concerned that it's beyond the design parameters of the
instrument and may leave us with a crippled coater (and a crippled lab).
We don't have the funds readily available to replace this machine if it
dies. Normally we run this instrument between 10 and 20 mA for 15
seconds to 3 minutes for SEM coating.

In terms of purging the chamber with argon, etc., this turbo pumped
EmiTech K575X runs on a programmed, automated cycle. The user's role is
to punch in the parameters and push the start button. It has been a
very reliable instrument after some initial setup problems were
corrected. We hope it remains so!

Thanks again, everybody.

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu









This incoming email to UWE has been independently scanned for viruses
and any virus detected has been removed using McAfee anti-virus software


This email has been independently scanned for viruses and any virus
software has been removed using McAfee anti-virus software



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 08:46:58 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 06 Apr 2005 10:01:11 -0700
Subject: [Microscopy] Re: Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I have an Epson Perfection 3200 and I just put the negative, emulsion
side up (yes up) on the bed and scan it as if it were a negative
transparency. Works fine. Dedicated film scanners that will do 3.25x4
film cost thousands of dollars.
Geoff

} } } } Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6/04/2005 3:54:32 a.m. } } }
} } } }
} } } }
} -----------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 08:57:51 2005



From: Stacey.Andringa-at-uc.edu (by way of MicroscopyListserver)
Date: Wed, 6 Apr 2005 09:13:52 -0500
Subject: [Microscopy] viaWWW: Fun stories about EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, April 6, 2005 at 06:26:44
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Microscopy] [Filtered] MListserver:Fun stories about EM

Question: Several of us were inspired by a previous listserv response
to collect interesting, funny, and odd stories about
our experiences in microscopy (especially those
historical and hysterical ones) to create a small compendium
to submit to one of the microscopy journals, or just to circulate
for nostalgic reading. Any and all stories are
welcome. If you contribute and want your name
withheld for some reason, please advise. If you have
imges (tifs, jpgs, gifs, or psds)they are welcome too.
Have fun, thanks, and have a great day, Marian L. Miller

Stacey Andringa
Marian Miller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 09:57:43 2005



From: frank.karl-at-degussa.com
Date: Wed, 6 Apr 2005 11:13:08 -0400
Subject: [Microscopy] I got 'em TEM blues....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,
I'm hoping I can get some advise on my Philips 430 TEM.

The problem appears that the filament, condenser aperture, specimen (lets
not forget those little guys)and objective aperture are not alined. I have
walked through the alinement procedure several times, but I continue to see
some odd artifacts:

At relatively low (6300X) mag I still see the edge of my objective aperture
in the plane of the specimen (I don't normally see this at this mag).

Going through cross-over I get a "dark field image" of my specimen
surrounding the bight field image on what appears to be an image of the
condenser aperture.

With the objective aperture out, I get a relatively even and circular
opening and closing cross over. When I put the objective aperture in the
cross over becomes egg shaped cross over. Centering either the objective
or condenser aperture doe's not seem to resolve the problem.

I have these problems with the specimen in or out.

Please feel free to contact me directly, if you think the response doesn't
merit the bandwidth.

Thanks........

Frank.karl-at-degussa.com


Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 10:15:53 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 6 Apr 2005 10:31:19 -0500
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives - many thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I would just like to thank everyone for their advice and comments on this
scanner question that I posted. I was able to research it fairly well in a
very short period of time.

I find this mailing list a huge and valuable resource to answer questions
like this, esp. when it comes to rapidly changing technology. I ended up
going with the Epson Perfection 4870 Photo because several people
recommended it, and the specs looked good, and the price was also very
reasonable, and I would be able to fit my negatives into the 4X5" holders
putting them crossways. I don't have it yet but people here have made me
feel confident about my decision. Thanks again.

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 11:10:59 2005



From: Ray D. Twesten :      twesten-at-uiuc.edu
Date: Wed, 6 Apr 2005 11:26:19 -0500
Subject: [Microscopy] RE: Si and O in lacey/holey C film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I have on occasion seen batches of grids that are contaminated with
silicone. Most EM people will eschew silicone based vacuum greases and
fluids, but I do not know if all the makers of carbon films are as careful.
The times that I have seen this, the manufacture was happy to replace the
grids.

I have also seen hydrocarbon solvents that are contaminated with silicon.
The result is the grid is clean until a user places their suspended sample
on the grid. Using a bare grid will confirm this.

If you have silicone contamination and you focus a small spot on the sample,
you will see a rapidly growing contamination spot. EDS will verify this
spot is Si and O rather than carbon.

Hope this helps, Ray

Ray D. Twesten, PhD.
Center for Microanalysis of Materials
Seitz Materials Research Laboratory
104 S. Goodwin Ave., Urbana, IL 61801
+1 217 244-6177 (Fax -2278)


} -----Original Message-----
} From: hkonishi-at-indiana.edu [mailto:hkonishi-at-indiana.edu]
} Sent: Tuesday, April 05, 2005 5:46 PM
} To: microscopy-at-microscopy.com
} Subject: [Microscopy] Si and O in lacey/holey C film
}
}
}
}
} ------------------------------------------------------------------
} ------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------
} -------------
}
} Hello,
}
} I am wondering why Si and O are detected from commercial C films (two
} companies). I measured C film and empty area (hole) next to the
} film, and I
} only detected Si and O from C film. I think that Si and O really
} come from C
} film.
}
} I think that C was coated on plastic film with small holes. Commercial
} lacey/holey C films are pure C or C on plastic. I don't think
} that plastic
} contains Si.
}
} I would appreciate any suggestion you might have.
}
} Thank you,
} Hiromi Konishi
} IU and JHU
}



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 11:23:37 2005



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Wed, 6 Apr 2005 12:39:16 -0400
Subject: [Microscopy] Jet Polishers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We are in the market for a twin (preferably) jet polisher for TEM foils.
I searched the List Server archives and found that this question was
discussed in 1997.
Is there any improvement since then?
The players then were:
Fischione
South Bay
Struers.
Anyone have any experience with any of them?
The South Bay unit looks suspiciously like the Struer's unit anyone
know who actually makes it?
Anyone have recommendations or horror stories?

Email please and I'll summarize if there are other parties interested.

John Mansfield PhD CPhys CSci MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143 USA
Phone: (734) 936-3352
FAX (734) 763-2282
Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"
AIM: thejfmjfm
Yahoo: thejfmjfm
Skype: thejfmjfm, (preferred)





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 11:34:33 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 6 Apr 2005 10:09:14 -0700
Subject: [Microscopy] Re: I got 'em TEM blues....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Garry

I would seriously looked at the Microtek type of flatbed scanner. It is
similar to the old AGFA Duoscan having a standard A4+ glass flatbed and
a similar size glassless film drawer with glassless holders for
negatives and transparencies up to A4. Check out the i900 below:
http://www.microtekusa.com/smi900.html

or other dual scan glassless flatbeds:
http://www.microtekusa.com/pp.html

I have been using the Microtek Scanmaker 8700 for some time and have
been very impressed, yet it's specification is blown away by the new
Microtek. It appears to be offering 3200x6400dpi resolution, 4.2 Dmax
(ie dense negatives and 48 bit colour - 16 bit B&W). I still use the
original 4x5 inch glassless holders on my 3.25 x 4inch negatives but I
am planning to get a special holder made up.

The only annoying quibble that I have is the i900 system comes with
digital 'ICE' for reduction of dust and scratches but this is only
available for prints - not negatives. So it may be worth checking if
there is a version that has 'ICE' for negatives/transparencies (in
fairness I think that only the Epson 4700 has 'ICE' on a normal
flatbed).

The i900 is about 600 US dollars (I,ve seen an advert for 60 dollars
less as well) but that's a lot cheaper than a true dedicated large film
scanner. I don't know how the prices compare in the USA with the Epson
4700 but the Epson is copying negatives on a glass bed which may mark.
gather dust or create Newtons's Rings.

I have no connection with Microtek other than as a satisfied user and I
was very happy with my old Epson 1200 scanner before that.


Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk




----- Original Message -----
} From: Geoff McAuliffe {mcauliff-at-umdnj.edu}


On Apr 6, 2005, at 8:13 AM, frank.karl-at-degussa.com wrote:

} I'm hoping I can get some advise on my Philips 430 TEM.
}
} The problem appears that the filament, condenser aperture, specimen
} (lets
} not forget those little guys)and objective aperture are not alined. I
} have
} walked through the alinement procedure several times, but I continue
} to see
} some odd artifacts:
}
} At relatively low (6300X) mag I still see the edge of my objective
} aperture
} in the plane of the specimen (I don't normally see this at this mag).
}
} Going through cross-over I get a "dark field image" of my specimen
} surrounding the bight field image on what appears to be an image of the
} condenser aperture.
}
} With the objective aperture out, I get a relatively even and circular
} opening and closing cross over. When I put the objective aperture in
} the
} cross over becomes egg shaped cross over. Centering either the
} objective
} or condenser aperture doe's not seem to resolve the problem.
}
} I have these problems with the specimen in or out.
}
} Please feel free to contact me directly, if you think the response
} doesn't
} merit the bandwidth.
}
Dear Frank,
From your description it looks like the filament, condenser aperture
and specimen are OK. I surmise this from the fact that with the
objective aperture out the beam spot expands evenly on both sides of
crossover, and that you do not report image motion when the condenser
is changed. I also assume that if the magnification were much
different from the indicated value, you'd have noticed and reported it,
and that you are not in some weird defocus condition (although this
would account for the dark-field-surrounding-bright-field effect, which
sounds somewhat like defocussed diffraction). Having the objective
aperture visible in the specimen plane sounds like a low mag condition,
where the objective aperture becomes an area-selecting aperture, and I
wonder if the "egg shaped cross over" is the edge of the objective
aperture occluding the beam. Have you checked all the lens currents?
If some of them are not at the usual values for the microscope
settings, there may be problems with the electronics. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 11:44:40 2005



From: MIKO(Lianfeng) :      lffu-at-ucdavis.edu
Date: Wed, 6 Apr 2005 10:00:26 -0700 (PDT)
Subject: [Microscopy] POSTDOCTORAL POSITIONS AVAILABLE IN UC-DAVIS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


POSTDOCTORAL POSITIONS IN INTERFACE PHYSICS

DEPARTMENT OF CHEMICAL ENGINEERING AND MATERIALS SCIENCE
UNIVERSITY OF CALIFORNIA-DAVIS

3 postdoctoral positions are available in the Interface Physics Group at
the University of California-Davis (UCD). Research in the Interface
Physics Group focuses on the use of atomic resolution imaging/analytical
techniques and newly developed dynamic measurement capabilities (sub-ns
time resolution) in electron microscopy, coupled with theoretical
simulations, to determine the structure-property relationships in nanoscale
systems. The positions that are currently available are related to high-Tc
superconductors, semiconductor quantum dots and the development of
nanoscale programs for the dynamic TEM. Successful candidates will be
recent Ph.D. graduates in physics, metallurgy, or materials science with a
sound background in the relevant materials issues and an ambition to be
part of a developing program pushing at the frontiers of interface
physics. Please send a resume and publication list to Professor Nigel D.
Browning at the address below. Prior experience in STEM or TEM is
essential. However, consideration will be based on the candidates overall
potential for success in the field and applicants with prior experience in
related fields are encouraged to apply. Positions are for one year
initially, normally renewed for a second year with possibilities existing
for further years. Salary is commensurate with experience.


Nigel D. Browning

Department of Chemical Engineering and Materials Science
University of California-Davis
One Shields Ave
Davis, CA 95616

AND

National Center for Electron Microscopy, MS 72-150
Lawrence Berkeley National Laboratory
Berkeley, CA 94720

Tel: 530-754-5358 (Davis)
510-486-4612 (NCEM)
Fax: 530-752-9554 (Davis)
510-486-5888 (NCEM)
e-mail: nbrowning-at-ucdavis.edu
ndbrowning-at-lbl.gov







From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 12:29:21 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 6 Apr 2005 13:45:59 -0400
Subject: [Microscopy] Re: Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Actually, that's what we do too with our Epson 3200--works nicely.

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu]
Sent: Wednesday, April 06, 2005 1:01 PM
To: microscopy-at-microscopy.com

I have an Epson Perfection 3200 and I just put the negative, emulsion
side up (yes up) on the bed and scan it as if it were a negative
transparency. Works fine. Dedicated film scanners that will do 3.25x4
film cost thousands of dollars.
Geoff

} } } } Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6/04/2005 3:54:32 a.m. } } }
} } } }
} } } }
} -----------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America







From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 12:54:15 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 06 Apr 2005 14:10:05 -0500
Subject: [Microscopy] Automatic vs manual desiccator storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Michael Zemyan wrote:
=========================================================
In reading the description of these automatic dessicators, I notice that
they have 2 fans, one of which continually circulates air inside the chamber
Does anyone know, are the fan bearings oil-lubricated? Is this
potentially a source of oil vapor contamination? We use dessicators (not
the automatic kind) to store cleaned vacuum parts, and so oil is a concern.
===========================================================
With regard to the Secador Automatic desiccator cabinets, see URL
http://www.2spi.com/catalog/supp/secador-desiccator-cabinets-4-0-vertical.
shtml

A ball bearing fan is used and these ball bearings are never oiled, however
they do contain oil. We have never heard of any reports however of anyone
reporting oil contamination of otherwise clean parts after sitting in the
environment of a Secador cabinet. I guess the acid test would be to do XPS
on similar parts stored, manual desiccator vs. automatic.

If you do this experiment, please share your results with us!

Disclaimer: SPI Supplies offers the Secador automatic desiccating cabinets,
both automatic as well as "manual" so if you don't like the automatic
feature, we would be happy to have you consider the manual version!

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 13:14:53 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.berkeley.edu
Date: Wed, 6 Apr 2005 11:30:31 -0700 (PDT)
Subject: [Microscopy] sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Since the topic of sputter coating has come up, I had a question I was
wondering about. We have a Balzers/technotrade MED020 sputter coater.
When coating, sometimes I have trouble generating the plasma. It will go
through arcing. If I adjust the argon flow to give a good amount of argon
in the chamber I can usually get the plasma to ignite better and have more
stability, then I can turn the argon flow down. Sometimes though, the
plasma will not ignite at all. I'm stumped by the problem and was
wondering if anyone had some advice?

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 13:33:42 2005



From: Yang, Ann-Fook :      YANGA-at-agr.gc.ca
Date: Wed, 6 Apr 2005 14:46:38 -0400
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives - many thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I find that the 4" X 5" film holder is clumsy to use. I had asked my machinist to cut two 31/4" X 4" holes, each with a 1 mm thick lip and a cut- out for lifting the negative, out of a black plastic sheet. You may have four holes cut from a plastic sheet; I chose two because I was afraid that four holes would weaken the plastic too much.
Placing the negatives on the glass is workable, but, one may get Newton rings.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Wednesday, April 06, 2005 11:31 AM
To: microscopy-at-microscopy.com


I would just like to thank everyone for their advice and comments on this
scanner question that I posted. I was able to research it fairly well in a
very short period of time.

I find this mailing list a huge and valuable resource to answer questions
like this, esp. when it comes to rapidly changing technology. I ended up
going with the Epson Perfection 4870 Photo because several people
recommended it, and the specs looked good, and the price was also very
reasonable, and I would be able to fit my negatives into the 4X5" holders
putting them crossways. I don't have it yet but people here have made me
feel confident about my decision. Thanks again.

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 13:42:51 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 6 Apr 2005 13:58:27 -0500
Subject: [Microscopy] New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in purchasing a new Ultramicrotome for sectioning in
diagnostic pathology to replace an aging Reichert that has been giving us
some problems. I have used Reichert Ultramictrotomes for the last 21 years,
and now they have become Leica, but I was wondering what opinions that
people have other other ultramicrotomes from other manufacturers, especially
new ultramicrotomes, such as the Powertome X or XO from RMC Products. Are
there other ultramicrotomes on the market that I should know about?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 14:24:59 2005



From: Lois Anderson :      landers-at-jhmi.edu
Date: Wed, 06 Apr 2005 15:39:33 -0400
Subject: [Microscopy] Re: Automatic vs manual desiccator storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We are very much interested in an automated film desiccator. Does
anyone have one or know who manufactures one?

Lois Anderson
Johns Hopkins University
Dept. of Pathology
Laboratory Manager
EM/IF/Reference Histology
600 N. Wolfe Street/Pathology 709 A
Baltimore, MD 21287
(410) 955-2861/fax (410) 614-7110
landers-at-jhmi.edu



} } } "Garber, Charles A." {cgarber-at-2spi.com} 04/06/05 3:10 PM } } }


------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Michael Zemyan wrote:
=========================================================
In reading the description of these automatic dessicators, I notice
that
they have 2 fans, one of which continually circulates air inside the
chamber
Does anyone know, are the fan bearings oil-lubricated? Is this
potentially a source of oil vapor contamination? We use dessicators
(not
the automatic kind) to store cleaned vacuum parts, and so oil is a
concern.
===========================================================
With regard to the Secador Automatic desiccator cabinets, see URL
http://www.2spi.com/catalog/supp/secador-desiccator-cabinets-4-0-vertical.
shtml

A ball bearing fan is used and these ball bearings are never oiled,
however
they do contain oil. We have never heard of any reports however of
anyone
reporting oil contamination of otherwise clean parts after sitting in
the
environment of a Secador cabinet. I guess the acid test would be to do
XPS
on similar parts stored, manual desiccator vs. automatic.

If you do this experiment, please share your results with us!

Disclaimer: SPI Supplies offers the Secador automatic desiccating
cabinets,
both automatic as well as "manual" so if you don't like the automatic
feature, we would be happy to have you consider the manual version!

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com

West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 14:39:47 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 06 Apr 2005 15:35:04 -0500
Subject: [Microscopy] Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gordon:

If I remember correctly, you can look to confirm this yourself, that the
argon inlet is at the bottom of the chamber NOT anywhere near the
sputterhead, imagine that! I found that most of the argon was going right
down the throat of the TMP.. The fix is to get a small SST tube that will
fit snuggly into the Argon inlet aperture and bend it around so it goes
around all of the other hardware in the chamber and end up about 1 cm lower
than the sputter head when sealed to the bell jar. This should do the trick
by actually putting the gas where it's needed, an advanced concept my
friends from "Happy Valley" didn't think of when designing the beast.

Should you care to discuss it further feel free to give me a call.

Cheers!


Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com


----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.berkeley.edu}
To: {microscopy-at-msa.microscopy.com}
Sent: Wednesday, April 06, 2005 2:30 PM

HI, Garry,

Leica, which is the company which acquired the Reichert microtome business
as part of the Cambridge-Leitz merger in 1990, came out last year with the
UC6. I had a chance to see it "up close and personal" at Cell Bio last
December and to work with it a little as part of a new AFM/ultramicrotome
integration for both bio and polymer applications. The same people who
sold your Reichert (ex: Ann Korsen) are with Leica and can answer your
questions. Ann can be reached at Akorsen-at-leica-microsystems.com

Hope this is helpful.

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to
learn more about "Optimizing LIght Microscopy". Copies still available
through MME... even for class-room lots ... and we give quantity discounts.



At 01:58 PM 4/6/2005, Garry Burgess wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 15:35:56 2005



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Wed, 6 Apr 2005 21:48:04 +0100
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Although I do have a vested interest, quite honestly I can't see that
'legal' protection for these terms achieves anything for anybody
apart from feeding the lawyers. In both cases, they are hardly more
than a simple description.

Personally, I'd suggest everybody use both as much as possible to
emphasise what nonsense it is.
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 16:35:08 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 06 Apr 2005 18:41:32 -0500
Subject: [Microscopy] Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

MKS http://www.mksinst.com/ was making a product in the past that might work
nicely. It is a tiny UV gas discharge lamp mounted in a standard vacuum
fitting. 120V AC, plugs into wall outlet. It is intended for starting up and
maintaining stable operation of cold cathode (Penning) vacuum sensors at
very low pressures (down to 10 -10 Torr). Description was "Cold Cathode
Starting Device", IgniTorr, part #100006850 (for 120V AC), used to cost
$220.

Even if it is discontinued, similar devices must be available from
semiconductor process tools suppliers (plasma etch, vac. vapor deposition,
etc.). In fact, any similar low power slow discharge UV lamp will work, just
find a way of placing it anywhere inside the process chamber. The one used
in IgniTorr has 2mA current and 55V breakdown voltage. It was connected to
120V AC line through 60K Ohm resistor. An isolation transformer wouldn't
hurt, plus, you will need only single pole vacuum connector.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane
Duluth, GA 30096
Tel. (770)232-7785
Fax (770)232-1791
Mobile (678)467-0012
www.sia-cam.com
----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.berkeley.edu}
To: {microscopy-at-msa.microscopy.com}
Sent: Wednesday, April 06, 2005 2:30 PM

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Larry Stoter wrote:
=================================================================
Although I do have a vested interest, quite honestly I can't see that
'legal' protection for these terms achieves anything for anybody apart from
feeding the lawyers. In both cases, they are hardly more than a simple
description.

Personally, I'd suggest everybody use both as much as possible to emphasise
what nonsense it is.
==================================================================
A trade name is someone's intellectual property right. It is analogous to
ownership in a patent.

It is also the only way a customer has of differentiating between products
of one brand vs. another (which indeed might have entirely different
properties and qualities). In microscopy, keeping track of brand identity
could be the difference in being able to reproduce someone else's results.
Indeed the major use of trade names on this listserver would show that as an
industry we do understand the importance of recognizing brand differences.

I would respectfully maintain that respecting someone's trade name is not
"nonsense". And to not respect such intellectual property rights and use
trade names incorrectly, is also illegal in most countries.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 20:07:51 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 6 Apr 2005 18:22:48 -0700
Subject: [Microscopy] Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Chuck;
Maybe a parallel could be seen in the auto industry where they
have a larger legal budget. Did auto company ever register 'Dual
Exhausts'? Dual is just a simple adjective, and to claim exclusive right
to that would be to hijack the English language. 'Magic Tip', on the
other hand, is a delightfully creative assembly of words, and
registering that does not hijack the English language. Anyway, who knows
how it could be decided when too many lawyers get involved. Witness the
spat in the courts now over Smucker's attempt to patent the peanut
butter and jelly sandwich!

http://www.msnbc.msn.com/id/7408857/

John Mardinly

-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: Wednesday, April 06, 2005 4:42 PM
To: MICROSCOPY BB

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Larry Stoter wrote:
=================================================================
Although I do have a vested interest, quite honestly I can't see that
'legal' protection for these terms achieves anything for anybody apart
from
feeding the lawyers. In both cases, they are hardly more than a simple
description.

Personally, I'd suggest everybody use both as much as possible to
emphasise
what nonsense it is.
==================================================================
A trade name is someone's intellectual property right. It is analogous
to
ownership in a patent.

It is also the only way a customer has of differentiating between
products
of one brand vs. another (which indeed might have entirely different
properties and qualities). In microscopy, keeping track of brand
identity
could be the difference in being able to reproduce someone else's
results.
Indeed the major use of trade names on this listserver would show that
as an
industry we do understand the importance of recognizing brand
differences.

I would respectfully maintain that respecting someone's trade name is
not
"nonsense". And to not respect such intellectual property rights and
use
trade names incorrectly, is also illegal in most countries.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================






From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 20:11:57 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 06 Apr 2005 18:29:06 -0700
Subject: [Microscopy] Re: Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I had terrible experience with Ann Korsen - she basically did not returned
telephone calls and ignored my E.mails. At one single instance when I had
a chance to talk to her, she was quite helpless and rigid. I am sorry,
this is my personal experience on the way to purpose new ultratome. I also
had multiple problems a couple years ago when my UCT needed
service. Finally, some problems were resolved (not in my favor to be
truthful, on some I just gave up) but I still have a feeling that Leica's
personnel was sort of unfriendly to me. To me, Leica provides miserable 5%
"educational" discount (as a huge favor to me), they do not exchange/return
stuff at all: illuminator I ordered for UC6 appeared to be for UCT and they
denied to exchange (unopened box) or get it back. Spare-parts box for UCT
was broken, it took more than year for Leica to replace it. Knifemaker was
at least a month late than promised... In my last purpose, they were trying
to "sweet" my experience with Leica including some free stuff. Paul
DeGeorge was quite nice after all, I appreciate it. Nevertheless, having
terrible customer service (from my experience), they have superior
instruments. I also have to admit that I never ever had any negative
experience (only positive) with people from RMC - they are extremely
helpful and flexible. I don't have any interest in Leica or RMC: I am
using the products from both companies. Sergey

At 01:35 PM 4/6/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 21:31:23 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 06 Apr 2005 21:47:16 -0500
Subject: [Microscopy] Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have a student who is trying to grow cultured cells on coverslips to use
them for SEM imaging. However, the cells either do not stay on the
coverslips when they are transported to our lab or tend to lift during
preparation for SEM. Growth conditions, etc are below.

Cell media: Dulbecco's Mod. Eagle Medium, 4.5g/L glucose, Na pyruvate, L-
glutamine. The cells are grown in the presence of 10% FBS, but the FBS is
removed from the media the day before the experiment.

Coverslips: Thermanox plastic coverslips, cell culture treated on one side,
purchased from VWR. Glass coverslips have also been tried and actually
worked better than the thermanox.

Cells: Caco-2 cells, grown for 8-10 days prior to conducting experiment.

Fixation is with 3% glutaraldehyde in phosphate buffer followed by 1%
osmium, ETOH series and critical pint dry. Samples are handled very gently
when solutions are changed.

We usually have little trouble with cultured cells but this one has been
nothing but problems. Suggestions for growth or SEM prep would be
appreciated.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 21:44:43 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 06 Apr 2005 20:00:34 -0700
Subject: [Microscopy] Re: RE: Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The issue is the trademark as it is registered.

The holder has a responsibility to defend the trademark.
If not, the mark is lost. If I call my company Intel or
Microsoft or AMD or Micron, or whatever, is that OK?

No. These are registered trademarks and are not useable
without attribution. And they cannot be usurped or owned.

The trademark has other more powerful yet obscure values.
A registered trademark that is a TLD overpowers any one
else trying to use it as a domain name. This is of course
based on the TM or TLD being registered before an attempted
infringement.

Trademarks are valuable and costly. They are to be protected.
I have one and I protect it.

gary g.


At 06:22 PM 4/6/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 00:27:19 2005



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Thu, 7 Apr 2005 07:47:07 +0200
Subject: [Microscopy] RE: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Debby
Quite some time ago I was involved sorting the a similar problem for a Masters degree Student, Denise Lindsey in microbiology at WITS University, Johannesburg, South Africa. She work on bio films. Might work equally well for Caco-2 cells. I am not sure if she ever published the sample preparation technique. We resorted to polished Stainless steel squares with a small hole in one corner. The surface finish was polished to 1200# SiC grinding paper. If the surface is to rough you do not get a good view the cells and if it is to smooth you loose to many cells during processing. To that we tied a thin string through the whole to handle it through the fixation and Dehydration process. Worked beautifully. They are reusable and have some direct industrial application in the case of biofilms. Give less charging problems in conventional SEM. Apparently she finished a PhD and is still in academia. The best person to contact is her former supervisor Prof. Alex von Holy. He should be able to get you in contact with her.
His e-mail is: alex-at-gecko.biol.wits.ac.za
I am also copying him in the mail.
Hope this helps

} -----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
} Sent: Thursday, April 07, 2005 4:47 AM
} To: message to: MSA list
} Subject: [Microscopy] Cultured cell prep for SEM
}
}
}
}
} --------------------------------------------------------------
} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} -----------------
}
} Hi all,
}
} I have a student who is trying to grow cultured cells on
} coverslips to use
} them for SEM imaging. However, the cells either do not stay on the
} coverslips when they are transported to our lab or tend to lift during
} preparation for SEM. Growth conditions, etc are below.
}
} Cell media: Dulbecco's Mod. Eagle Medium, 4.5g/L glucose, Na
} pyruvate, L-
} glutamine. The cells are grown in the presence of 10% FBS,
} but the FBS is
} removed from the media the day before the experiment.
}
} Coverslips: Thermanox plastic coverslips, cell culture
} treated on one side,
} purchased from VWR. Glass coverslips have also been tried and actually
} worked better than the thermanox.
}
} Cells: Caco-2 cells, grown for 8-10 days prior to conducting
} experiment.
}
} Fixation is with 3% glutaraldehyde in phosphate buffer followed by 1%
} osmium, ETOH series and critical pint dry. Samples are
} handled very gently
} when solutions are changed.
}
} We usually have little trouble with cultured cells but this
} one has been
} nothing but problems. Suggestions for growth or SEM prep would be
} appreciated.
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 02:27:46 2005



From: Victoria Fink :      vfink-at-shaw.ca
Date: Thu, 07 Apr 2005 00:43:12 -0700
Subject: [Microscopy] Re: RE: Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Gary,

Completely agree. I believe when somebody selecting trademark, or name of
the company a few optional names should be provided by the applying person.
You should pay for the name search prior a registration. And they usually
checking up our options. If none of them will not go through you will need
to do this again. I would suggest to check out already known names on
similar products to avoid to pay again. You should be creative enough to
select this name. If you have created product, I sure you will be able to
create a name for it. This is not necessary should be name of your product,
it could be name of your lovely person ( as "Mercedes" did) , or anything
you like, or anything you have invented in this model, any language: classic
ones, bible, anything. When you designed your product you did it with
passion as usually creative people do. Try to concentrate on the one or two
worlds you would express your passion, or what does it mean for you, or your
customers benefit/s. Good luck!

Victoria

----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
Sent: Wednesday, April 06, 2005 8:00 PM



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 04:36:24 2005



From: yezer-at-cc.hut.fi
Date: Thu, 07 Apr 2005 12:50:55 +0300 (EEST)
Subject: [Microscopy] HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I am using Hitachi S-4700 FESEM (cold tip) and so far didn’t succeed to achieve
a SE image with resolution better than 10 nm (including in the ultra high-
resolution mode). Can anyone help us with a guide lines how to reach high
resolution by controlling the parameters (Ie, Cond lens, and etc) with this
microscope?.

Thanks,

E. Yossef
Helsinki University of Technology
Physical Metallurgy and Materials Science


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 05:40:46 2005



From: George Theodossiou :      George.Theodossiou-at-amcor.com.au
Date: Thu, 7 Apr 2005 17:47:38 +1000
Subject: [Microscopy] Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Or the bitter fight over ownership of the term 'Ugg Boots'. That
delightfully australian sheepskin footwear that is oh so comfortable and oh
so warm.



-----Original Message-----
} From: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Thursday, 7 April 2005 11:23
To: Garber, Charles A.; MICROSCOPY BB

Chuck;
Maybe a parallel could be seen in the auto industry where they have
a larger legal budget. Did auto company ever register 'Dual Exhausts'? Dual
is just a simple adjective, and to claim exclusive right to that would be to
hijack the English language. 'Magic Tip', on the other hand, is a
delightfully creative assembly of words, and registering that does not
hijack the English language. Anyway, who knows how it could be decided when
too many lawyers get involved. Witness the spat in the courts now over
Smucker's attempt to patent the peanut butter and jelly sandwich!

http://www.msnbc.msn.com/id/7408857/

John Mardinly

-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: Wednesday, April 06, 2005 4:42 PM
To: MICROSCOPY BB

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Larry Stoter wrote:
=================================================================
Although I do have a vested interest, quite honestly I can't see that
'legal' protection for these terms achieves anything for anybody apart from
feeding the lawyers. In both cases, they are hardly more than a simple
description.

Personally, I'd suggest everybody use both as much as possible to emphasise
what nonsense it is.
==================================================================
A trade name is someone's intellectual property right. It is analogous to
ownership in a patent.

It is also the only way a customer has of differentiating between products
of one brand vs. another (which indeed might have entirely different
properties and qualities). In microscopy, keeping track of brand identity
could be the difference in being able to reproduce someone else's results.
Indeed the major use of trade names on this listserver would show that as an
industry we do understand the importance of recognizing brand differences.

I would respectfully maintain that respecting someone's trade name is not
"nonsense". And to not respect such intellectual property rights and
use
trade names incorrectly, is also illegal in most countries.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================






************************************************************************
CAUTION - This message may contain privileged and confidential
information intended only for the use of the addressee named above.
If you are not the intended recipient of this message you are hereby
notified that any use, dissemination, distribution or reproduction of
this message is prohibited. If you have received this message in error
please notify AMCOR immediately.
Any views expressed in this message are those of the individual sender
and may not necessarily reflect the views of AMCOR.
************************************************************************



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 06:19:58 2005



From: ech :      ech-at-interchange.ubc.ca
Date: Thu, 07 Apr 2005 04:35:56 -0700 (PDT)
Subject: [Microscopy] Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Garry
we have three OM3s, an Ultracut E and an Ultracut T, all Leica. For the International Cryo EM Course last year, Leica brought in the UC6 which we got to thoroughly test. We really liked it.

They have made so many improvements on it that we (by that I actually mean George Postuma, Philip Hyam and Paul de George) easily had the course participants cutting cryo sections in a very short time. Three UC6's were bought by new faculty at UBC for their own labs in the next year and I believe them to be satisfied customers. These don't have cryo capability; they use our cryo system if they need it. Since we have George at the cryo course, we have been using the Tokuyasu method in our lab a lot more. He makes it very easy. The advantages of course being that the immunogold penetrates the whole section as there is no resin to get in the way. We used to have a lot of trouble with this method until George came from Jan Slot's lab gave us his experience.

We have cryo cutting ability on the Ultracut E, but the ease of use of the Ultracut E and UC6 is night and day. Leica are bringing UC6s to the cryo course this year. One thing we hope to try is high pressure freezing, cryo planing (UC6) and transfering onto the cryo stage of the cryo sem. We will also be trying the new Bal-Tec cryo transfer device to go from the high pressure freezer to the cryo-sem. I haven't advertised the cryo course on the website this year as there are only two places left. This year Helmut Gnaegi from Diatome will also be coming to teach cutting techniques.

I am sorry that I have no experience of the other products that you mentioned. Will you be going to the Microscopy Society of Canada meeting in Hamilton in May? Maybe we can touch base then as I would love to network and find out about these other products.

As you are in Canada, I guess Philip Hyam is your Leica rep too. We have always had very good dealings with him. We have someone in Vancouver who is very good at servicing our microtomes which is an added benefit and probably the most important thing about buying a new piece of equipment. At least for me running a facility.



----Original Message-----

} Date: Wed Apr 06 11:58:27 PDT 2005
} From: "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca}
} Subject: [Microscopy] New Ultramicrotomes
} To: "Microscopy MSA" {microscopy-at-microscopy.com}
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} I am interested in purchasing a new Ultramicrotome for sectioning in
} diagnostic pathology to replace an aging Reichert that has been giving us
} some problems. I have used Reichert Ultramictrotomes for the last 21 years,
} and now they have become Leica, but I was wondering what opinions that
} people have other other ultramicrotomes from other manufacturers, especially
} new ultramicrotomes, such as the Powertome X or XO from RMC Products. Are
} there other ultramicrotomes on the market that I should know about?
}
} This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 06:32:55 2005



From: ech :      ech-at-interchange.ubc.ca
Date: Thu, 07 Apr 2005 04:48:53 -0700 (PDT)
Subject: [Microscopy] Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Garry
we have three OM3s, an Ultracut E and an Ultracut T, all Leica. For the International Cryo EM Course last year, Leica brought in the UC6 which we got to thoroughly test. We really liked it.

They have made so many improvements on it that we (by that I actually mean George Postuma, Philip Hyam and Paul de George) easily had the course participants cutting cryo sections in a very short time. Three UC6's were bought by new faculty at UBC for their own labs in the next year and I believe them to be satisfied customers. These don't have cryo capability; they use our cryo system if they need it. Since we have George at the cryo course, we have been using the Tokuyasu method in our lab a lot more. He makes it very easy. The advantages of course being that the immunogold penetrates the whole section as there is no resin to get in the way. We used to have a lot of trouble with this method until George came from Jan Slot's lab and gave us his experience.

We have cryo cutting ability on the Ultracut E, but the ease of use of the Ultracut E and UC6 is night and day. Leica are bringing UC6s to the cryo course this year in June. One thing we hope to try is high pressure freezing, cryo planing (UC6) and transfering onto the cryo stage of the cryo sem. We will also be trying the new Bal-Tec cryo transfer device to go from the high pressure freezer to the cryo-sem. I haven't advertised the cryo course on the website this year as there are only two places left. This year Helmut Gnaegi from Diatome will also be coming to teach cutting techniques.

I am sorry that I have no experience of the other products that you mentioned. Will you be going to the Microscopy Society of Canada meeting in Hamilton in May? Maybe we can touch base then as I would love to network and find out about these other products.

As you are in Canada, I guess Philip Hyam is your Leica rep too. We have always had very good dealings with him. We have someone in Vancouver who is very good at servicing our microtomes which is an added benefit and probably the most important thing about buying a new piece of equipment. At least for me running a facility.
Elaine
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} I am interested in purchasing a new Ultramicrotome for sectioning in
} diagnostic pathology to replace an aging Reichert that has been giving us
} some problems. I have used Reichert Ultramictrotomes for the last 21 years,
} and now they have become Leica, but I was wondering what opinions that
} people have other other ultramicrotomes from other manufacturers, especially
} new ultramicrotomes, such as the Powertome X or XO from RMC Products. Are
} there other ultramicrotomes on the market that I should know about?
}
} This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
}
--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 07:29:48 2005



From: Richard Edelmann :      edelmare-at-MUOhio.edu
Date: Thu, 7 Apr 2005 08:44:57 -0400
Subject: [Microscopy] Re: Trade names: was: "Dualbeam" "Crossbeam"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

With respect to Charles and a few other vendors, I agree with Larry and
John. A Unique name is very valuable and can serve a company exceedingly
well in recognition - and justifyable should be protected legally. If the name
comes be associated with a particular product or even better to be almost
synonymous with technique or product so much the better for the vendor - as
in Kleenex, or EDAX. But like “Miracle Tip” forceps, you will note that these
are unique names and NOT simply descriptive terms. Attempting to force
synonymy by trademarking or registering a description is “questionable” to
say the least. However, we at least have EDS, EDX, EDXS, XEDS, or even
EDXRF under which to list the technique used.

What I was hoping for was an answer from the folks who are actually
USING “dual column ,dual gun, sample milling and imaging FIB/SEM systems
“ on a term they use or even would LIKE to use for the technique. I have no
vested interest in the technology, I do not use the instruments, nor even write
about them, but in trying to assemble the buyers guide for the EXPO
supplement for this summers M&M meeting I just wanted a name to list the
vendors under rather than leaving the “products” out of the listing all together.


However, it has turned into an interesting discussion and I do hope the
vendors are listening. Secondly, it does raise the point that as a society of
real users perhaps we should be the ones whom tell the vendors what to call
things.

(With some apologies to the marketing folks at FEI and Zeiss for offending
or even EDAX)


} }
} } Folks:
} }
} } Help! I need a different name to call "Dualbeam" or "Crossbeam"
} } instruments since one is trademarked and the other is registered (FEI &
} } Zeiss).
} }
} }
} }
} } Richard E. Edelmann, Ph.D.
}
} Although I do have a vested interest, quite honestly I can't see that
} 'legal' protection for these terms achieves anything for anybody
} apart from feeding the lawyers. In both cases, they are hardly more
} than a simple description.
}
} Personally, I'd suggest everybody use both as much as possible to
} emphasise what nonsense it is.
} --
} Larry Stoter
} JEOL (UK) Ltd
} tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com
}
} PLEASE NOTE
} 1. Any mail other than plain text will be automatically deleted.
} 2. Any mail, legitimate or not, apparently or actually from hotmail,
} netscape, yahoo or excite will automatically be deleted.
} 3. Mail with no subject or without a clear subject will be ignored :-)
}



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 07:45:34 2005



From: Lesley Graham :      patljg-at-gwumc.edu
Date: Thu, 07 Apr 2005 09:00:50 -0400
Subject: [Microscopy] fibrin embedded cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Name: Lesley Graham

Organization: George Washington Univ.

Has anyone ever embedded cells in fibrin as opposed to agar? If so,
what protocol did you use?

Thank you,
Lesley


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 08:23:02 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 07 Apr 2005 09:38:14 -0400
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debby,
You may be making for some very unhappy cells by removing the FBS a
day ahead of fixation. Yes, you want to remove it so that it does
not get fixed to the surface of your cells, but I have found that
washing the cells with serum-free media 2-3 times just prior to
fixation is usually adequate and that way the cells are kept in their
"happy" conditions right up to the end.
When you say that the coversilps are cell culture treated, what does
that mean? Poly-l-lysine? Collagen? A different base might work
better. Caco-2 cells are big ugly things, but they are widely used so
you should be able to find references for conditions for good
adherence.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 08:33:25 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Apr 2005 08:48:35 -0500
Subject: [Microscopy] HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We also have this microscope and we can see 10nm immunogold label in
backscatter mode, so you should get much better than that in SE. For
highest resolution, we use the shortest working distances possible, high
KV's, and keep the stage lock on. We normally just use high-resolution
mode, but sometimes we manually set the condenser lenses to higher
values. Often, however, by doing this the image may become so noisy
that the increased resolution doesn't do any good, since you can't
really see it anyway. At close working distances we make sure the lower
detector is "off", since it contributes little or nothing to the signal.


The lower the noise level in the room, the better. Keep very quiet
during high-mag image recording, since we can often see noise vibration
from voices in the picture. Try to keep your vacuum pumps and air
compressor in another room, if possible. If that's not possible, try
to reduce vibrations from them by setting pumps on short lengths of
thick vacuum hose. Make sure the nitrogen dewar for the cold finger is
full and give it time to settle down---it will contribute significant
vibration for up to 15 minutes after filling.

For surface resolution on "softer", i.e., less dense samples, such as
most biological specimens, it may be better to use lower accelerating
voltages to limit generation of SE's from deeper inside the specimen.
This will increase contrast of surface features, which is not the same
as resolution, and will help keep them from being overwhelmed by
secondaries originating from deeper inside the sample.

One thing we have consistently noted in our instrument is that very
close working distances require more or less constant realignment of the
scope, including electronic aperture alignment and stigmation. We check
alignment at the highest mag possible for each and every image we
record. I don't know if this is a characteristic of these scopes, in
general, but it is certainly the case for us. A little error in
stigmation can really mess up an image at high magnification.

Use the lightest coatings of the finest grain metals possible. We
routinely use platinum to coat our samples, and for high-resolution work
we start with a light coat (say 10mA for 15-30 seconds in our coater)
and add more metal as needed until we get charging under control. You
can always add more, but removing a too-heavy layer is another story.
Chromium coating is another option for even finer grain, but the
oxidation problem limits the useful lifetime of a single coating. Or
one can carbon coat.

We find that every sample is unique, especially when trying for the
highest resolutions we can get. We often check several accelerating
voltages.

I am sure that other users of this scope have their own tricks and tips
for high-res work and Dr. David Joy and others teach a short course on
how to maximize the potential of these instruments.

We love this scope! It requires tweaking to get the best results, but
those results can be spectacular.

Hope this helps a little.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
} From: yezer-at-cc.hut.fi [mailto:yezer-at-cc.hut.fi]
Sent: Thursday, April 07, 2005 4:51 AM
To: Microscopy-at-microscopy.com

Hi all,

I am using Hitachi S-4700 FESEM (cold tip) and so far didn't succeed to
achieve a SE image with resolution better than 10 nm (including in the
ultra high- resolution mode). Can anyone help us with a guide lines how
to reach high resolution by controlling the parameters (Ie, Cond lens,
and etc) with this microscope?.

Thanks,

E. Yossef
Helsinki University of Technology
Physical Metallurgy and Materials Science




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 09:03:49 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Thu, 07 Apr 2005 09:19:01 -0500
Subject: [Microscopy] Re: Re: RE: Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

getting back to the original issue, which was raised by larry stoter, i
believe.

for those of us who also work with molecular biology, there is a
prominant example of the importance of trade names and what can happen
if we fail to protect them. i refer to the TaqMan assay system. i
suspect most of us have heard of TaqMan, and 'the TaqMan machine'. this
system for (RT-)PCR, which utilizes 5' nuclease sensitive
oligonucleotides for probes, was developed by Perkin Elmer and used
prominantly in marketing and use of their 7100 realtime thermocycler
system. the way i understand the story, several years ago a competitor,
Roche, realized that the name had not been trademarked. they registered
the name. technically, now you can only use the name when you are
referring to realtime (RT)-PCR using a Roche system, while those who
popularized the concept have been left out in the cold.

we should all remember this type of story when the issue of trade names
arises, along with the consequences of failure to adequately protect
them. personally, i view the merchandising of scientific discoveries
with a great deal of distaste, and from the notes suspect larry stoter
has a similar view. but the issue of recognition is important. we need
to know that our peers know what we do and recognize us. it is
important in our scientific careers. we depend upon protection through
referencing our publications, copyright protection against plagerism,
and legal protection against theft of intellectual property. like it or
not, tradenames are as important to the business community as our
publications and recognition guaranteed by copyrights are to us.

note, i have no interest in any companies, molecular, EM, or otherwise,
but have used 5'-nuclease probes with various systems. to avoid any
dispute and try to be fair to all, i make it clear that i refer to the
system as "5'-nuclease probe" regardless of what method or
manufacturer's system i am using to test for a molecular target.

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 09:39:11 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Thu, 07 Apr 2005 09:54:08 -0500
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Debby,

First, try fixing with 1.25% glut + 1% tannic acid. This should
improve the cells' structures, especially the membrane. Also, when we
do SEM of cultured cells, we typically don't use OsO4. No need, and
it can interfere with visualizing colloidal metal labels with BSE
imaging. When I have used OsO4 with cells, I again add 1% tannic acid
to the OsO4.
I'm not surprised the cells stick better to glass, though. They may
stick even better to gold. If you have a 100% Au target in your
sputter coater, or gold wire for your vacuum evaporator, you might
try gold-coating the glass or Thermanox and then growing the cells.
Caco-2 ... the only time I've done these was using transwells in
multi-well plates, 1.25% glut in serum (and other protein) free
buffer (organics free is better, like Na/Na2 PO4, as the organics
tend to fix into an obscuring goo over the cells), no OsO4. The
Caco-2 cells stuck to the transwell membranes very nicely. Given the
way OsO4 can harden cells and tissues, this may be your problem.
These wells also allow experiments where the cells have different
environments on the two surfaces (basal and lumenal). Give the
transwells a try.

Phil
P.S. When you "critical pint dry", what pint do you use? I tend to
prefer Elijah Craig.


} Hi all,
}
} I have a student who is trying to grow cultured cells on coverslips to use
} them for SEM imaging. However, the cells either do not stay on the
} coverslips when they are transported to our lab or tend to lift during
} preparation for SEM. Growth conditions, etc are below.
}
} Cell media: Dulbecco's Mod. Eagle Medium, 4.5g/L glucose, Na pyruvate, L-
} glutamine. The cells are grown in the presence of 10% FBS, but the FBS is
} removed from the media the day before the experiment.
}
} Coverslips: Thermanox plastic coverslips, cell culture treated on one side,
} purchased from VWR. Glass coverslips have also been tried and actually
} worked better than the thermanox.
}
} Cells: Caco-2 cells, grown for 8-10 days prior to conducting experiment.
}
} Fixation is with 3% glutaraldehyde in phosphate buffer followed by 1%
} osmium, ETOH series and critical pint dry. Samples are handled very gently
} when solutions are changed.
}
} We usually have little trouble with cultured cells but this one has been
} nothing but problems. Suggestions for growth or SEM prep would be
} appreciated.
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 09:59:15 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Thu, 07 Apr 2005 10:14:57 -0500
Subject: [Microscopy] Re: sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gordon,

I find that with our Balzers MED 010, which is basically the same
thing, I have to have the pressure at 2X10^-5 mbar to 1X10^-1 mbar
routinely, as was given this value by the good folks at TechnoTrade.
As to arcing and never getting the plasma to ignite, the only times
I've had this happen, it was cured by opening up the chamber and lid
and cleaning everything, including under the sputtering target.
Especially around the target.

Phil

} Hello,
} Since the topic of sputter coating has come up, I had a question I
} was wondering about. We have a Balzers/technotrade MED020 sputter
} coater. When coating, sometimes I have trouble generating the
} plasma. It will go through arcing. If I adjust the argon flow to
} give a good amount of argon in the chamber I can usually get the
} plasma to ignite better and have more stability, then I can turn the
} argon flow down. Sometimes though, the plasma will not ignite at
} all. I'm stumped by the problem and was wondering if anyone had
} some advice?
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak
} Electron Microscope Lab
} AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 10:01:26 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 07 Apr 2005 11:10:18 -0400
Subject: [Microscopy] Re: Re: Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Well, not everyone has had bad experiences with Ann Korsen.
She has brought us instruments on loan last fall, and was most
helpful and generous towards us, as well as easily reachable
at all times. Overall my experience with Leica has always been
most pleasant. Yes, they do offer small rebates on instruments,
but in the end you get what you pay for! All the experts in the
field of cryo-ultramicrotomy will tell you that there is only one
machine on the market worth considering. I experienced this
first-hand at a EMBO course in Paris last September, when
we had the choice between Leica Ultracut microtomes and
RMC. Yes, maybe RMC works well when you know how to
use it, but there was no doubt in my mind (and that of the other
instructors on the course) that the Leica machine is beautifully
designed, user-friendly and reliable, at least as far as cryo-sectioning
is concerned. So OK, one company will cost much more and
give smaller rebates, but if you had the choice between a
Yugo with a 25% rebate, or a Mercedes with no rebate, and
money was not an issue, what would you buy?!!
And like you, I don't have any special interests in either
company. I'm just a very happy Leica customer!

Marc


On Wednesday, April 6, 2005, at 09:29 PM, Sergey Ryazantsev wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I had terrible experience with Ann Korsen - she basically did not
} returned telephone calls and ignored my E.mails. At one single
} instance when I had a chance to talk to her, she was quite helpless
} and rigid. I am sorry, this is my personal experience on the way to
} purpose new ultratome. I also had multiple problems a couple years
} ago when my UCT needed service. Finally, some problems were resolved
} (not in my favor to be truthful, on some I just gave up) but I still
} have a feeling that Leica's personnel was sort of unfriendly to me.
} To me, Leica provides miserable 5% "educational" discount (as a huge
} favor to me), they do not exchange/return stuff at all: illuminator I
} ordered for UC6 appeared to be for UCT and they denied to exchange
} (unopened box) or get it back. Spare-parts box for UCT was broken, it
} took more than year for Leica to replace it. Knifemaker was at least a
} month late than promised... In my last purpose, they were trying to
} "sweet" my experience with Leica including some free stuff. Paul
} DeGeorge was quite nice after all, I appreciate it. Nevertheless,
} having terrible customer service (from my experience), they have
} superior instruments. I also have to admit that I never ever had any
} negative experience (only positive) with people from RMC - they are
} extremely helpful and flexible. I don't have any interest in Leica or
} RMC: I am using the products from both companies. Sergey
}
} At 01:35 PM 4/6/2005, you wrote:
}
}
} } ----------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ---------
} }
} } HI, Garry,
} }
} } Leica, which is the company which acquired the Reichert microtome
} } business as part of the Cambridge-Leitz merger in 1990, came out last
} } year with the UC6. I had a chance to see it "up close and personal"
} } at Cell Bio last December and to work with it a little as part of a
} } new AFM/ultramicrotome integration for both bio and polymer
} } applications. The same people who sold your Reichert (ex: Ann
} } Korsen) are with Leica and can answer your questions. Ann can be
} } reached at Akorsen-at-leica-microsystems.com
} }
} } Hope this is helpful.
} }
} } Best regards,
} } Barbara Foster
} }
} } Microscopy/Microscopy Education
} } 313 S Jupiter Rd, Suite 100
} } Allen, TX 75002
} } P: 972-954-8011
} } W: www.MicroscopyEducation.com
} }
} } P. S.
} } Need a good general reference or light microscopy text? Call us today
} } to learn more about "Optimizing LIght Microscopy". Copies still
} } available through MME... even for class-room lots ... and we give
} } quantity discounts.
} }
} }
} }
} } At 01:58 PM 4/6/2005, Garry Burgess wrote:
} }
} }
} } } ---------------------------------------------------------------------
} } } ---------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ---------------------------------------------------------------------
} } } ----------
} } }
} } }
} } } I am interested in purchasing a new Ultramicrotome for sectioning in
} } } diagnostic pathology to replace an aging Reichert that has been
} } } giving us
} } } some problems. I have used Reichert Ultramictrotomes for the last
} } } 21 years,
} } } and now they have become Leica, but I was wondering what opinions
} } } that
} } } people have other other ultramicrotomes from other manufacturers,
} } } especially
} } } new ultramicrotomes, such as the Powertome X or XO from RMC
} } } Products. Are
} } } there other ultramicrotomes on the market that I should know about?
} } }
} } } This e-mail and/or any documents in this transmission is intended
} } } for the address(s) only and may contain legally privileged or
} } } confidential information. Any unauthorized use, disclosure,
} } } distribution, copying or dissemination is strictly prohibited. If
} } } you receive this transmission in error, please notify the sender
} } } immediately and return the original.
} }
} }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 10:13:21 2005



From: Lesley Weston :      leswes-at-shaw.ca
Date: Thu, 07 Apr 2005 08:28:27 -0700
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Would it be possible for the student to glow-discharge the coverslips
(either glass or Thermanox) before plating the cells? This would make them
much more adhesive and has the side-benefit of sterilising them as well. It
could affect the behaviour of the cells, but this might not matter,
depending on the experiment. Another possibility is to remove the FBS from
the medium closer to the time of the experiment, if possible. The presence
or absence of FBS has large effects on cell behaviour, possibly including
adhesion.

Lesley Weston.


} From: Debby Sherman {dsherman-at-purdue.edu}
} Date: Wed, 06 Apr 2005 21:47:16 -0500
} To: "message to: MSA list" {microscopy-at-MSA.microscopy.com}
} Subject: [Microscopy] Cultured cell prep for SEM
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
--} -
}
} Hi all,
}
} I have a student who is trying to grow cultured cells on coverslips to use
} them for SEM imaging. However, the cells either do not stay on the
} coverslips when they are transported to our lab or tend to lift during
} preparation for SEM. Growth conditions, etc are below.
}
} Cell media: Dulbecco's Mod. Eagle Medium, 4.5g/L glucose, Na pyruvate, L-
} glutamine. The cells are grown in the presence of 10% FBS, but the FBS is
} removed from the media the day before the experiment.
}
} Coverslips: Thermanox plastic coverslips, cell culture treated on one side,
} purchased from VWR. Glass coverslips have also been tried and actually
} worked better than the thermanox.
}
} Cells: Caco-2 cells, grown for 8-10 days prior to conducting experiment.
}
} Fixation is with 3% glutaraldehyde in phosphate buffer followed by 1%
} osmium, ETOH series and critical pint dry. Samples are handled very gently
} when solutions are changed.
}
} We usually have little trouble with cultured cells but this one has been
} nothing but problems. Suggestions for growth or SEM prep would be
} appreciated.
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 10:22:17 2005



From: Robert A Underwood :      underwoo-at-u.washington.edu
Date: Thu, 7 Apr 2005 08:37:47 -0700 (PDT)
Subject: [Microscopy] picking up ultrathin cryosections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Fellow Microscopists,

I have a question about picking up ultrathin cryosections. I have notice when I am using very lightly
fixed tissue, many of the sections look like the tissue is stretching apart, leaving small holes
throughout. I thought at first that this was just due to lack of fixation, however I could find a ocassional
section/grid that was amazingly better. This indicated that the pickup is a major factor. I have tried
sucrose vs. methylcellulose/sucrose vs. methylcellulose/sucrose/UA and find that it is still just in the
pickup that some are better than others. Do you have some advice on how to pickup the sections to
avoid this type of artifact?
Thank you for any advice.

Robert Underwood
Research Scientist
University of Washington
Seattle, WA USA







From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 11:08:53 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 07 Apr 2005 12:24:01 -0400
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

another nice thing about Transwells (for TEM): if you cut the filter
from the holder before dehydration, when you put them into Proplyene
oxide they curl up like a jelly roll. You can then embed them in a
flat mold and cut cross sections of the roll so that you get many
more cell profiles/section than if you'd cut a strip of the membrane
and just embedded that.
For SEM, I leave them intact and put them into the CPD. You just
have to keep track of which one is which.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 11:13:28 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Thu, 07 Apr 2005 12:28:18 -0400
Subject: [Microscopy] Re: fibrin embedded cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We were using this technique around 1970 when I was a grad student. It was
published by a guy named J. Furtado and it may have been published in
MYCOLOGIA. That is the best I can do for you with out some more digging.
It worked quite nicely to keep fungal spores in place during
ptocessing.

Good luck on finding it and let me know.

Greg

At 09:00 AM 4/7/2005, Lesley Graham wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 11:26:35 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Apr 2005 11:41:50 -0500
Subject: [Microscopy] HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

In my earlier reply to this, I forgot to mention one other factor that
affects the final quality of the digital image produced by our S4700. A
peculiarity of our instrument, and I have seen at least one other
mention of this on the listserv, is that when an image from the scope is
opened in Adobe Photoshop it comes up in "Indexed Color" mode. I have
no idea why. If this image is converted into 8-bit greyscale mode it
gets cleaned up quite a bit in terms of "noise"---the difference is
sometimes striking.

Granted this has nothing to due with the resolution of the image in the
microscope, but it does have a lot to do with the appearance of the
final presentation image.

Is anyone else familiar with this?

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
} From: yezer-at-cc.hut.fi [mailto:yezer-at-cc.hut.fi]
Sent: Thursday, April 07, 2005 4:51 AM
To: Microscopy-at-microscopy.com

Hi all,

I am using Hitachi S-4700 FESEM (cold tip) and so far didn't succeed to
achieve a SE image with resolution better than 10 nm (including in the
ultra high- resolution mode). Can anyone help us with a guide lines how
to reach high resolution by controlling the parameters (Ie, Cond lens,
and etc) with this microscope?.

Thanks,

E. Yossef
Helsinki University of Technology
Physical Metallurgy and Materials Science




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 11:37:16 2005



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Thu, 7 Apr 2005 12:52:07 -0700
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

HI, Debby-
I don't know why anyone would want to use cover slips. We grow them
on the regular plastic dishes they are used to. These samples are
easy to embed and the cell layer separates readily from the dish if
the procedure is performed correctly. If yu give me a FAX number, I
shall send you a protocol This one is not on our web site (yet).
Carol


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu
http://www.bgsu.edu/departments/biology/facilities/MnM
___________________________________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 12:35:58 2005



From: pgrover :      pgrover-at-bilbo.bio.purdue.edu
Date: Thu, 7 Apr 2005 12:51:57 -0500
Subject: [Microscopy] re: Fun stories about EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm sure I'm not alone in my fond memories of the cool micrographs that used
to grace the cover of the Journal of Irreproducible Results (and maybe I'm
not the only one to drop my subscription when they stopped doing it). Does
anyone know whether there exists a compendium of those cover images? Or any
other collection of weird & wonderful micrographs?

Paul Grover

----------------------------------------------------------------------
"Keep your eyes slightly wide and blank. Show no interest or excitement."
- Dr. Miles Bennell
Invasion of the Body Snatchers (1956)

(Opinions expressed here are not necessarily those of my employer or of
Purdue University)








From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 12:51:18 2005



From: Peter Ingram :      p.ingram-at-voice.cellbio.duke.edu
Date: Thu, 7 Apr 2005 14:07:01 -0400
Subject: [Microscopy] Trademarks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below you will find a short comment from a lawyer on
trademarks, copyrights and patents that might clarify - or confuse! -
the issue. In any event if there is enough interest I am sure the
MSA Public Policy committee could organize a discussion on trademarks
at the Hawaii meeting this August.

If nyone is interested, please respond to me with the word
"Trademaks" in the Subject line so that a) the Listserv and b) my
regular email don't get unecessarily cluttered!

Peter Ingram

MSA Legal Liaison

Comments:

The two (trademarks and copyrights) are wholly different species of
intellectual property, and both are distinct from patents. Each is
governed by statute, but copyright and trademark law also has a large
add-on of "common law" arising out of both state statute and decided
cases. An overview/introduction to the
three categories of intellectual property staes that there are actually "3.5
categories" - the ".5" is a very different, but very interesting, area
of "trade secrets." These are largely non-statutory and, most
interesting, based on exactly the opposite legal basis of the other
three - that is, copyright, trademark and patent laws depend on public
disclosure, publicity, to establish their strengths - patents, as you
know, mandate full public disclosure, in filings with the US Patent
office, which are publicly available - in order for the patent to
"issue." The reason for this is that patent protection is limited in
time; after the patent time expires, all are free to exactly duplicate
the instructions in the patent. Trademarks are not similarly limited in
time, but may be lost by failure to protect them adequately
--
Peter Ingram
Duke University Medical Center
Box 90319
LaSalle Street Extension
DURHAM NC USA 27708-0319

Tel: (919) 660-2695
Fax: (919) 660-2671
e-mail: p.ingram-at-cellbio.duke.edu



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 12:56:48 2005



From: Paul Webster :      pwebster-at-hei.org
Date: Thu, 07 Apr 2005 11:10:06 -0700
Subject: [Microscopy] Re: picking up ultrathin cryosections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Robert,

You have come across an interesting observation with cryosections. As you
know cryosections are not embedded in a continuous, polymerized plastic
sheet, as are resin-embedded sections. The only physical bonds holding the
structure together are those protein interactions that may remain after the
tissue has been treated, and cross-linking bonds formed by the aldehyde
fixation. Of course, less fixation will mean fewer cross-linking bonds to
hold the sections together.

To cut the story short, the variability you are seeing in the section
morphology is most probably due to surface tension effects on the surface of
the pick-up drop. Reduce the surface tension and you will improve the
section morphology.

Warm pick-up solutions will spread the sections more than frozen drops of
pick-up solution. More methyl cellulose in the sucrose appears also to
reduce the surface tension. You could also try adding gelatin to the sucrose
to lower the surface tension.

You could also experiment with the time taken to collect the sections from
the knife. You may find that as you get better at picking them up, the
morphology will get worse. If this is so, slow down the process and give the
pick-up solution time to cool down. Once you get a good result, stick with
the protocol.

Alternatively, you could add a very small amount of glutaraldehyde to your
fixative. This may toughen up the sections enough to improve the morphology.

Finally, make sure that you are not drying the sections during the labeling
process. If you are in the habit of taking off excess buffer before you
float that grids on antibody you may be drying them enough to cause
morphology changes. Thin sections are very sensitive to drying.

I am sure that you are aware that you can take your frozen, cryoprotected
blocks and either warm them up again for embedding in epoxy resin. This has
always been a good way to convince people that poor fixation or freezing is
not the reason for poor morphology in cryoprotected frozen material.

You can also take the frozen blocks of sucrose-infiltrated material and
freeze substitute in cold methanol. This is also a good way of checking the
morphology if the material is then embedded in epoxy resin.

Regards,

Paul Webster.




Paul Webster, Ph.D.
House Ear Institute
2100 West Third Street
Los Angeles
CA 90057-1922



On 4/7/05 8:37 AM, "Robert A Underwood" {underwoo-at-u.washington.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Hi Fellow Microscopists,
}
} I have a question about picking up ultrathin cryosections. I have notice when
} I am using very lightly
} fixed tissue, many of the sections look like the tissue is stretching apart,
} leaving small holes
} throughout. I thought at first that this was just due to lack of fixation,
} however I could find a ocassional
} section/grid that was amazingly better. This indicated that the pickup is a
} major factor. I have tried
} sucrose vs. methylcellulose/sucrose vs. methylcellulose/sucrose/UA and find
} that it is still just in the
} pickup that some are better than others. Do you have some advice on how to
} pickup the sections to
} avoid this type of artifact?
} Thank you for any advice.
}
} Robert Underwood
} Research Scientist
} University of Washington
} Seattle, WA USA
}
}
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 13:01:45 2005



From: Jay Campbell :      microtomy-at-gmail.com
Date: Thu, 7 Apr 2005 13:16:30 -0500
Subject: [Microscopy] Re: Re: Re: Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

"Yes, maybe RMC works well when you know how to
use it"

In my experience this is pretty important no matter what
brand/piece of equipment you are talking about. Some people have a
preference based on what they are "used to" that may have little or no
bearing on the quality of the competing product.
Jay

On Apr 7, 2005 10:10 AM, Marc Pypaert {marc.pypaert-at-yale.edu} wrote:
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Well, not everyone has had bad experiences with Ann Korsen.
} She has brought us instruments on loan last fall, and was most
} helpful and generous towards us, as well as easily reachable
} at all times. Overall my experience with Leica has always been
} most pleasant. Yes, they do offer small rebates on instruments,
} but in the end you get what you pay for! All the experts in the
} field of cryo-ultramicrotomy will tell you that there is only one
} machine on the market worth considering. I experienced this
} first-hand at a EMBO course in Paris last September, when
} we had the choice between Leica Ultracut microtomes and
} RMC. Yes, maybe RMC works well when you know how to
} use it, but there was no doubt in my mind (and that of the other
} instructors on the course) that the Leica machine is beautifully
} designed, user-friendly and reliable, at least as far as cryo-sectioning
} is concerned. So OK, one company will cost much more and
} give smaller rebates, but if you had the choice between a
} Yugo with a 25% rebate, or a Mercedes with no rebate, and
} money was not an issue, what would you buy?!!
} And like you, I don't have any special interests in either
} company. I'm just a very happy Leica customer!
}
} Marc
}
}
} On Wednesday, April 6, 2005, at 09:29 PM, Sergey Ryazantsev wrote:
}
} }
} }
} } -----------------------------------------------------------------------
} } -------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------
} } --------
} }
} } I had terrible experience with Ann Korsen - she basically did not
} } returned telephone calls and ignored my E.mails. At one single
} } instance when I had a chance to talk to her, she was quite helpless
} } and rigid. I am sorry, this is my personal experience on the way to
} } purpose new ultratome. I also had multiple problems a couple years
} } ago when my UCT needed service. Finally, some problems were resolved
} } (not in my favor to be truthful, on some I just gave up) but I still
} } have a feeling that Leica's personnel was sort of unfriendly to me.
} } To me, Leica provides miserable 5% "educational" discount (as a huge
} } favor to me), they do not exchange/return stuff at all: illuminator I
} } ordered for UC6 appeared to be for UCT and they denied to exchange
} } (unopened box) or get it back. Spare-parts box for UCT was broken, it
} } took more than year for Leica to replace it. Knifemaker was at least a
} } month late than promised... In my last purpose, they were trying to
} } "sweet" my experience with Leica including some free stuff. Paul
} } DeGeorge was quite nice after all, I appreciate it. Nevertheless,
} } having terrible customer service (from my experience), they have
} } superior instruments. I also have to admit that I never ever had any
} } negative experience (only positive) with people from RMC - they are
} } extremely helpful and flexible. I don't have any interest in Leica or
} } RMC: I am using the products from both companies. Sergey
} }
} } At 01:35 PM 4/6/2005, you wrote:
} }
} }
} } } ----------------------------------------------------------------------
} } } --------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------
} } } ---------
} } }
} } } HI, Garry,
} } }
} } } Leica, which is the company which acquired the Reichert microtome
} } } business as part of the Cambridge-Leitz merger in 1990, came out last
} } } year with the UC6. I had a chance to see it "up close and personal"
} } } at Cell Bio last December and to work with it a little as part of a
} } } new AFM/ultramicrotome integration for both bio and polymer
} } } applications. The same people who sold your Reichert (ex: Ann
} } } Korsen) are with Leica and can answer your questions. Ann can be
} } } reached at Akorsen-at-leica-microsystems.com
} } }
} } } Hope this is helpful.
} } }
} } } Best regards,
} } } Barbara Foster
} } }
} } } Microscopy/Microscopy Education
} } } 313 S Jupiter Rd, Suite 100
} } } Allen, TX 75002
} } } P: 972-954-8011
} } } W: www.MicroscopyEducation.com
} } }
} } } P. S.
} } } Need a good general reference or light microscopy text? Call us today
} } } to learn more about "Optimizing LIght Microscopy". Copies still
} } } available through MME... even for class-room lots ... and we give
} } } quantity discounts.
} } }
} } }
} } }
} } } At 01:58 PM 4/6/2005, Garry Burgess wrote:
} } }
} } }
} } } } ---------------------------------------------------------------------
} } } } ---------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } } America
} } } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } } ---------------------------------------------------------------------
} } } } ----------
} } } }
} } } }
} } } } I am interested in purchasing a new Ultramicrotome for sectioning in
} } } } diagnostic pathology to replace an aging Reichert that has been
} } } } giving us
} } } } some problems. I have used Reichert Ultramictrotomes for the last
} } } } 21 years,
} } } } and now they have become Leica, but I was wondering what opinions
} } } } that
} } } } people have other other ultramicrotomes from other manufacturers,
} } } } especially
} } } } new ultramicrotomes, such as the Powertome X or XO from RMC
} } } } Products. Are
} } } } there other ultramicrotomes on the market that I should know about?
} } } }
} } } } This e-mail and/or any documents in this transmission is intended
} } } } for the address(s) only and may contain legally privileged or
} } } } confidential information. Any unauthorized use, disclosure,
} } } } distribution, copying or dissemination is strictly prohibited. If
} } } } you receive this transmission in error, please notify the sender
} } } } immediately and return the original.
} } }
} } }
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } 10833 Le Conte Ave, Room 33-080
} } Los Angeles, CA 90095
} }
} } Phone: (310) 825-1144 (office)
} } (310) 206-1029 (Lab)
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
} }
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 13:21:10 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 07 Apr 2005 14:35:57 -0400
Subject: [Microscopy] Re: picking up ultrathin cryosections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If it makes you feel better, most of us are struggling with the
very same problem! So I am very interested in the responses
you are going to receive to your question.

I used to think that when picking up, either with sucrose alone
or sucrose/methyl cellulose, one had to be extremely quick, and
the sections had to magically lift up onto the still liquid drop in
which it would disappear as if they were dissolving in the sucrose.
But talking to others since then, I have come to realize that
being too fast can actually harm the sections, by over-stretching
them and making holes and tears appear. One person told me
that she waits until the rim of the loop of sucrose/methylcellulose
just starts freezing (a white ring appearing at the periphery of
the droplet) before picking up the sections. Given the speed
at which sucrose/methylcellulose freezes, this often means that
by the time the sections have been picked up and the loop
retrieved from the chamber, the whole drop is now frozen. You
just have to wait for it to thaw again, then transfer the sections on
the grid. But this technique hasn't worked too well in my hands
so far - I get too many folds. There are too many factors that
are involved here: size of the loop (we use a very small one -
smaller diameter than a grid), percentage of sucrose and methyl
cellulose (I use 50/50), temperature of the chamber (-108°C
as opposed to -120°C), the use of gelatin to embed samples,
and of course the fixation protocol. But anyway, you should
maybe give this a try and see if waiting a few more sections
during pick up, so that the surface of your drop is close to
freezing point, might help prevent some of the tearing.

By the way, what do you call "very slightly fixed", and why
do you have to fix so lightly?

Best

Marc


On Thursday, April 7, 2005, at 11:37 AM, Robert A Underwood wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Hi Fellow Microscopists,
}
} I have a question about picking up ultrathin cryosections. I have
} notice when I am using very lightly fixed tissue, many of the sections
} look like the tissue is stretching apart, leaving small holes
} throughout. I thought at first that this was just due to lack of
} fixation, however I could find a ocassional section/grid that was
} amazingly better. This indicated that the pickup is a major factor. I
} have tried sucrose vs. methylcellulose/sucrose vs.
} methylcellulose/sucrose/UA and find that it is still just in the
} pickup that some are better than others. Do you have some advice on
} how to pickup the sections to avoid this type of artifact?
} Thank you for any advice.
}
} Robert Underwood
} Research Scientist
} University of Washington
} Seattle, WA USA
}
}
}
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 13:29:05 2005



From: bbandli :      bbandli-at-mvainc.com
Date: Thu, 07 Apr 2005 14:44:41 -0400
Subject: [Microscopy] Re: RE: HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Our JEOL6500-F (JEOL PCSEM software) has the same indexed color
"feature" and converting it to 8-bit greyscale has the same effect of
cleaning up the noise in the image. I also have no idea why.

Bryan Bandli

Tindall, Randy D. wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 14:29:40 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Apr 2005 14:45:26 -0500
Subject: [Microscopy] Re: Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I feel I need to chime in on this, since my experiences with Leica have
been wonderful. I have dealt with Ann Korsen, Paul DeGeorge, Robert
Seiler, and others in the company in both sales and training situations
and have no complaints about their knowledge, willingness to work with
the customer, or followup on any problems that might occur. This has
also been true of their distributors, in our case Mager Scientific.

That said, I have also had very pleasant dealings with the RMC crew
during demonstrations and workshops. If I were buying a new
ultramicritome I would be making my equipment decisions based solely on
the equipment itself and the applications it will address, since both
companies field good people, in my opinion. Maybe I'm just
wishy-washy....

Our lab and I have no financial or other ties with any of these
companies, etc., but we do have two Leica UCT ultramicrotomes (one with
cryo), a Leica high-pressure freezer, and a Leica freeze-substitution
unit, so we have plenty of experience with this company.

Just my two copper-coated zinc plugs worth....

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu




-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, April 06, 2005 8:29 PM
To: Microscopy-at-microscopy.com

I had terrible experience with Ann Korsen - she basically did not
returned telephone calls and ignored my E.mails. At one single instance
when I had a chance to talk to her, she was quite helpless and rigid. I
am sorry, this is my personal experience on the way to purpose new
ultratome. I also had multiple problems a couple years ago when my UCT
needed service. Finally, some problems were resolved (not in my favor
to be truthful, on some I just gave up) but I still have a feeling that
Leica's personnel was sort of unfriendly to me. To me, Leica provides
miserable 5% "educational" discount (as a huge favor to me), they do not
exchange/return stuff at all: illuminator I ordered for UC6 appeared to
be for UCT and they denied to exchange (unopened box) or get it back.
Spare-parts box for UCT was broken, it took more than year for Leica to
replace it. Knifemaker was at least a month late than promised... In my
last purpose, they were trying to "sweet" my experience with Leica
including some free stuff. Paul DeGeorge was quite nice after all, I
appreciate it. Nevertheless, having terrible customer service (from my
experience), they have superior instruments. I also have to admit that
I never ever had any negative experience (only positive) with people
from RMC - they are extremely helpful and flexible. I don't have any
interest in Leica or RMC: I am using the products from both companies.
Sergey

At 01:35 PM 4/6/2005, you wrote:


} -----------------------------------------------------------------------
} ------- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver

} Cell Bio last December and to work with it a little as part of a new
} AFM/ultramicrotome integration for both bio and polymer applications.
} The same people who sold your Reichert (ex: Ann Korsen) are with Leica
} and can answer your questions. Ann can be reached at
} Akorsen-at-leica-microsystems.com
}
} Hope this is helpful.
}
} Best regards,
} Barbara Foster
}
} Microscopy/Microscopy Education
} 313 S Jupiter Rd, Suite 100
} Allen, TX 75002
} P: 972-954-8011
} W: www.MicroscopyEducation.com
}
} P. S.
} Need a good general reference or light microscopy text? Call us today
} to learn more about "Optimizing LIght Microscopy". Copies still
} available through MME... even for class-room lots ... and we give
quantity discounts.
}
}
}
} At 01:58 PM 4/6/2005, Garry Burgess wrote:
}
}
} } ----------------------------------------------------------------------
} } -------- The Microscopy ListServer -- Sponsor: The Microscopy Society

} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver

} } us some problems. I have used Reichert Ultramictrotomes for the last
} } 21 years, and now they have become Leica, but I was wondering what
} } opinions that people have other other ultramicrotomes from other
} } manufacturers, especially new ultramicrotomes, such as the Powertome X

} } or XO from RMC Products. Are there other ultramicrotomes on the
market that I should know about?
} }
} } This e-mail and/or any documents in this transmission is intended for
} } the
} } address(s) only and may contain legally privileged or confidential
} } information. Any unauthorized use, disclosure, distribution, copying
} } or dissemination is strictly prohibited. If you receive this
} } transmission in error, please notify the sender immediately and return
the original.
}
}

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu







From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 15:17:34 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 08 Apr 2005 08:33:45 +1200
Subject: [Microscopy] EDS recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The best and simple thing to do is to go to the nearest business centre and
tell them that you want to register trademark on your product. They will
teach you what to do, how much it may cost, and how to do this properly.
Even they will recommend to you how to do this, and what start from, and you
will have an idea how much it will cost. They will refer you to appropriate
lawyers, and organization. Trademarks are associated with commercial, or
ready to sell products. Do you have it? If you have commercial product you
may wish to sell it by your own company. Then you may decide to register
your company, and name of your product could be associated with name of the
company. Just some ideas.
Victoria


----- Original Message -----
} From: "Peter Ingram" {p.ingram-at-voice.cellbio.duke.edu}
To: {microscopy-at-MSA.Microscopy.com}
Sent: Thursday, April 07, 2005 11:07 AM

Hi

I'm in the market for a new LN2-cooled, Be-window Si(Li) EDS detector, to go on a
JEOL 840, and to be used mainly for quantitative analysis of minerals.

The manufacturers that I'm currently considering are EDAX, Gresham, Thermo Noran,
and Rontec.

Anybody got any to add to this list?

Anybody got any reasonably valid bias towards or against any of these?

Confidentiality of off-list replies will be respected.

Please note that I'm looking for a detector/preamp only, I already have a very
satisfactory pulse-processor, mca, etc.


cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 18:16:26 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Fri, 08 Apr 2005 09:37:16 +0100
Subject: [Microscopy] Re: picking up ultrathin cryosections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the replies so far, keep 'em coming, but please note that I'm enquiring about
detectors only ie detector plus detector preamp, not systems (pulse-processor, MCA,
software etc).

cheers

rtch


} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
Organization: Dept of Geology, Univ of Auckland
To: microscopy-at-microscopy.com
Date sent: Fri, 08 Apr 2005 08:33:45 +1200

Dear Robert,

1) It would be better to use tissues fixed with a little percentage of
glutaraldehyde (0.1-0.2%). this will not kill much of antigenic epitops
but will greatly improve the ultrastructural stability of the sample.
You can find exact reference in the papers of P.J.Peters group in
Amsterdam or H.Geuse group in Utrecht.
2) In my hands the best solution for picking up is
Methylcellulose/sucrose. Sucrose alone has a very big surface tension,
which will eventually destroy sensitive structures as Golgi complex.
Addition of UA to the pick up solution can improve the ultrastructure
but can reduce labeling. You can try to add low concentration of tannic
acid (less than 0.1%) in solution. Sometimes it helps but be aware of
the effects on the epitopes for labeling.
3) The other thing to consider is timing of your pick up. It is very
critical to pick up at the moment when the solution just on the verge to
start to freeze. If you will pick up sections sooner or later then you
will probably end up with a horrible ultrastructure. It is very hard to
explain how to determine the right moment. I think it will be better
that you just try yourself several settings because our instuments for
pick up could be different from yours (loop diameter, wire thickness
etc.) and it will affect the timing. May be this movie from P.Peters'
lab will help:
http://129.112.18.40/cryomovies/

Sincerely,
Alex

Robert A Underwood:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Hi Fellow Microscopists,
}
} I have a question about picking up ultrathin cryosections. I have
} notice when I am using very lightly fixed tissue, many of the sections
} look like the tissue is stretching apart, leaving small holes
} throughout. I thought at first that this was just due to lack of
} fixation, however I could find a ocassional section/grid that was
} amazingly better. This indicated that the pickup is a major factor. I
} have tried sucrose vs. methylcellulose/sucrose vs.
} methylcellulose/sucrose/UA and find that it is still just in the
} pickup that some are better than others. Do you have some advice on
} how to pickup the sections to avoid this type of artifact?
} Thank you for any advice.
}
} Robert Underwood
} Research Scientist
} University of Washington
} Seattle, WA USA
}
}
}
}
}
}
}

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 04:31:43 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Fri, 8 Apr 2005 07:17:00 -0230
Subject: [Microscopy] RE: RE: HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Randy D. writes ...

} ... A peculiarity of our instrument, and I have seen at
} least one other mention of this on the listserv, is that
} when an image from the scope is opened in Adobe Photoshop
} it comes up in "Indexed Color" mode. I have no idea why.
} If this image is converted into 8-bit greyscale mode it
} gets cleaned up quite a bit in terms of "noise"---

Check this behaviour at Photoshop 100% magnification and above. I believe
that Photoshop blends pixels differently at lower view magnifications,
depending on whether the working space is color managed or not. An indexed
grayscale would not be color managed.

The better question would be to ask the manufacturuers for their reasons
why grayscales are indexed. Most do, and there is really no justification.

my $0.02 & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 07:09:35 2005



From: cbutterick-at-poco.com (by way of MicroscopyListserver)
Date: Fri, 8 Apr 2005 07:25:41 -0500
Subject: [Microscopy] viaWWW: Independent Service Contractor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cbutterick-at-poco.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, April 7, 2005 at 09:21:35
---------------------------------------------------------------------------

Email: cbutterick-at-poco.com
Name: Chuck Butterick

Organization: Poco Graphite, Inc

Title-Subject: [Microscopy] [Filtered] Independent Service Contract

Question: Is there an independent service engineer interested in servicing a DSM 962 (currently under contract) in Texas (DFW)?

Contact me off the Listserver, please.


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 07:10:15 2005



From: mcary-at-gatan.com (by way of MicroscopyListserver)
Date: Fri, 8 Apr 2005 07:26:06 -0500
Subject: [Microscopy] viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mcary-at-gatan.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, April 7, 2005 at 12:12:38
---------------------------------------------------------------------------

Email: mcary-at-gatan.com
Name: Matthew Cary

Organization: gatan inc

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: how do i most acuratly measure the ohms/square inch of an indium tin coated surface??

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 07:10:07 2005



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Fri, 08 Apr 2005 09:25:38 -0300
Subject: [Microscopy] Re: RE: RE: HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The same thing happens with the images from our JEOL JSM-5600. A real
annoyance since a lot of useful
things in Photoshop are disabled for indexed images. I usually convert
them wholesale to grayscale with a
Photoshop script. In the case of the 5600, I believe the images are
indexed so that you can apply the
color lookup table, etc. to the images within the GUI. A relatively
useless "eye candy" feature, in my
opinion, but then I'm not a salesman. Equally annoying is that the color
stuff that you can draw on the images
(measurement lines, etc.) are smashed down into grayscale when they are
written into the image! Why aren't
these colors preserved in the indexed image? Grrr! Anybody interested in
starting a thread about useless
and annoying "features" on scopes that could best be left out or
improved? Maybe the manufacturers are listening....

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



michael shaffer wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 08:43:49 2005



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Fri, 8 Apr 2005 10:24:57 -0400
Subject: [Microscopy] re new anything

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Yee Yan,

My understanding is that there are several factors involved in selecting
an accelerating voltage (KV). At higher KVs, chromatic aberration is
reduced, resulting in greater resolution, i.e., the ability to separate
two points visually. However, higher KVs also result in the generation
of secondary electrons from a larger surface area, due to primary beam
electron scattering over a larger lateral distance, as well as from
deeper within the specimen.

This theoretically has two effects. One is that the larger area of the
surface emitting SE's gives a larger "effective spot size", or area from
which signal is generated, and this decreases resolution. The other is
that the SEs escaping from deeper within the specimen are detected along
with the SEs generated at or just below the surface, and this part of
the signal can obscure surface detail by lowering contrast. This effect
can easily be seen by taking a biological specimen or other soft sample
and simply comparing a 1.0 KV image to a 30 KV image: the higher KV
image looks smoother and less detailed.

This effect is complicated by the effect of sample density, in that a
denser sample will have a smaller "effective spot size" than a
less-dense sample at the same KV. This means that for many samples you
can increase resolution by increasing KV if the sample is dense enough
to restrict the electron scattering to acceptable levels for the
magnification you are working at. In addition, higher KVs generally
allow such adjustments as using smaller apertures to decrease spherical
aberration effects and increase depth of field, and higher lens
currents to demagnify the probe size and increase resolution that way.
Generally speaking, adjusting the SEM for high resolution means
decreasing the signal to the detectors, so if you have more signal to
begin with, you can afford to lose more signal before noise overwhelms
your image.

I agree with you that for many samples, especially biological ones,
lower KVs are better at retaining surface detail in the image. We
routinely run our FESEM at 5.0 KV, and often at 1.0 KV. But not always.
Separating out the relative effects of larger "effective spot sizes"
(decreased resolution), escape of SE's from deeper within the specimen
(lower surface contrast), and decreased aberrations (increased
resolution) at high KV's is not straightforward. And let's not even get
into the effects of coatings on these factors, since you may need
heavier coatings to counter increased charging effects, as you
mentioned! As I said, it's probably best to approach each sample as
unique and experiment.

By copy of this to the listserv I am asking to be set straight on any
errors in the above sermon. I always learn a lot from these discussions
and have had faulty assumptions corrected on several occasions.

Cheers,
Randy

-----Original Message-----
} From: Tay Yee Yan [mailto:one_twinklestar-at-yahoo.com.sg]
Sent: Thursday, April 07, 2005 7:17 PM
To: Tindall, Randy D.

I have been getting increasingly disturbed by the extensive traffic
concerning both ultramicrotomes and personnel associated with companies
selling the product. Some observations after roughly 30 years in the
business of purchasing and maintaining both major instruments (TEM, SEM,
XPS, Auger, EPMA, SIMS, etc) and ancillary equipment (microtomes, polishers,
ion mills, etc):

1. for a given instrument most everyone with extensive experience possesses
'brand loyalty' to some degree, based on a combination of need,vs instrument
specs, a good price, reasonable expectation of good service and
user-friendlyness over a period of time.

2. even if the above has held true for some time, you never know when new
developments may occur, pricing policies may change (or can be forced to
change on a case-by-case basis), or sales and service personnel might change
(don't forget the former people as they often can influence the latter).
It's an ever-shifting field.

3. returning to #1, you never know when your needs may change, so be careful
about swearing too much loyalty to a particular brand (unless you have a
very stable capital budget and senior management that trusts you
completely!).

4. I've known a lot of company staff from sales, service and/or R&D, and
have met relatively few 'duds'. These people work very hard and in a
surprising number of cases are paid even less than we are. For certain they
have nowhere near our degree of autonomy, so please keep their names (pro or
con) off the air. I doubt any of us would appreciate our qualifications
and style being debated in public.

5. For that matter, why not simply keep specific product names off the
Listserver as much as possible? Give your candid impressions to the
inquirer off-line. If you feel the need to enlighten the Listserver at
large, confine your comments to more generic comments, "I find in my lab hat
a good X needs to have qualities A, B, C-- to get the job done".

6. As an example of #5, I have given some dozen workshops on hard materials
microtomy with both of the producers mentioned in the above thread.
Students have attended who have, or are considering, instruments from both
vendors. The focus has religously been on the technique and its potential
results, not the pros and cons of the products that can get you there, and
the results have been very pleasing to all concerned. In my opinion, this
is the prime purpose of the Listserver; education, not brand endorsement.

Tom

Dr. Tom Malis
Manager
Academic User Access Facility (AUAF)
CANMET Materials Technology Laboratory
Natural Resources Canada
558 Booth St., Ottawa, Ontario K1A 0G1
Tel.: (613) 995-7358 FAX: (613) 992-8735, cell: 613-371-4577
malis-at-nrcan.gc.ca {mailto:malis-at-nrcan.gc.ca}




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 09:43:18 2005



From: Linda Fox :      lfox1-at-lumc.edu
Date: Fri, 08 Apr 2005 09:58:20 -0500
Subject: [Microscopy] TEM digital plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Friends,

Is anyone using digital plates in place of film in their TEM's?

Can you let me know about cost, efficiency, resolution, practicality,
and any problems that you have had. Also what system you are using and
has the service been good.

We are looking into this option as one way to "go digital" with 2 old
TEM's. Also, does anyone still use film, but then scan the images into
a computer? That is another option that we are looking at.
Thanks,
Linda Fox
lfox1-at-lumc.edu

Linda M. Fox
Loyola University
Stritch School of Medicine
Core Imaging Facility
2160 S. First Ave.
Maywood, Il 60153
Bld. 102 Room 0617
1-708-216-3395
lfox1-at-lumc.edu



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 09:45:57 2005



From: Linda Fox :      lfox1-at-lumc.edu
Date: Fri, 08 Apr 2005 10:01:01 -0500
Subject: [Microscopy] Video Camera for LM scope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We are upgrading our image analysis system (Leitz Orthoplan interfaced to an Apple computer running OSX and ImageJ) and would appreciate any suggestions for a video camera with a screw mount. We currently have an older Javelin with a ½ inch chip, which has a thread mount on a tube (approx 7/8 in diameter) out the back port. We'd like to keep the tube so we don't need to worry about the optical path, working distance, etc.
Thanks,
John McNulty
jmcnulty-at-lumc.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 11:35:58 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 8 Apr 2005 10:07:04 -0700
Subject: [Microscopy] Re: viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 8, 2005, at 5:26 AM, by way of MicroscopyListserver wrote:

} Question: how do i most acuratly measure the ohms/square inch of an
} indium tin coated surface??
}
Dear Matthew,
There are two papers by Mike Lamvik (RS Rader & MK Lamvik (1992)
High-conductivity amorphous TiSi substrates for low-temperature
electron microscopy. J. Microsc. 168, 71-77. and MK Lamvik, SD
Davilla, and J Tuttle (1989) Properties of substrates for low
temperature quantitative microscopy and microanalysis. Scan. Microsc.
Suppl. 3 271-276.) that describe resistivity measurements of thin
films. If you can configure your coating as described in those papers,
that would be a way to measure the resistance accurately. If, however,
your coating is already prepared, and if it is not on an insulating
surface, you will have to go to greater lengths, and accurate
measurement may not be possible. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 11:37:49 2005



From: William H Roberts :      William.H.Roberts-at-USA.dupont.com
Date: Fri, 8 Apr 2005 12:53:10 -0400
Subject: [Microscopy] viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Matthew,

Surface resistivity has units of ohms per square (not per square inch). I
know that this sounds strange, but the dimensions drop out in the
calculations. There are a number of vendors who sell the circular
concentric electrodes which are used to measure the value, for example:
keithly and trek, to name just two. Also if you just Google on "surface
resistivity measurement" there are some very good papers that are available
in pdf format which will explain surface resistivity in much greater detail
than I can. There may even be some simple way of measuring the value
without the use of one of the commercial units (or without making one
yourself).

Bill




mcary-at-gatan.com (by way of MicroscopyListserver) on 04/08/2005 08:26:06 AM


To: microscopy-at-microscopy.com
cc:


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mcary-at-gatan.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday,
April 7, 2005 at 12:12:38
---------------------------------------------------------------------------

Email: mcary-at-gatan.com
Name: Matthew Cary

Organization: gatan inc

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: how do i most acuratly measure the ohms/square inch of an indium
tin coated surface??

---------------------------------------------------------------------------






This communication is for use by the intended recipient and contains
information that may be privileged, confidential or copyrighted under
applicable law. If you are not the intended recipient, you are hereby
formally notified that any use, copying or distribution of this e-mail,
in whole or in part, is strictly prohibited. Please notify the sender
by return e-mail and delete this e-mail from your system. Unless
explicitly and conspicuously designated as "E-Contract Intended",
this e-mail does not constitute a contract offer, a contract amendment,
or an acceptance of a contract offer. This e-mail does not constitute
a consent to the use of sender's contact information for direct marketing
purposes or for transfers of data to third parties.

Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean

http://www.DuPont.com/corp/email_disclaimer.html




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 12:24:25 2005



From: Gerroir, Paul :      paul.gerroir-at-xrcc.xeroxlabs.com
Date: Fri, 8 Apr 2005 13:38:35 -0400
Subject: [Microscopy] re new anything

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Tom,
Well said. Unfortunately there have been individuals who use this
listserver to make personal attacks publicly. Ultimately they are the
ones who suffer.

Paul


Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: 905-823-7091, ext.216
FAX: 905-822-7022
e-mail: paul.gerroir-at-xrcc.xeroxlabs.com

-----Original Message-----
} From: Malis, Tom [mailto:malis-at-NRCan.gc.ca]
Sent: Friday, April 08, 2005 10:25 AM
To: 'microscopy-at-microscopy.com'

I have been getting increasingly disturbed by the extensive traffic
concerning both ultramicrotomes and personnel associated with companies
selling the product. Some observations after roughly 30 years in the
business of purchasing and maintaining both major instruments (TEM, SEM,
XPS, Auger, EPMA, SIMS, etc) and ancillary equipment (microtomes,
polishers, ion mills, etc):

1. for a given instrument most everyone with extensive experience
possesses 'brand loyalty' to some degree, based on a combination of
need,vs instrument specs, a good price, reasonable expectation of good
service and user-friendlyness over a period of time.

2. even if the above has held true for some time, you never know when
new developments may occur, pricing policies may change (or can be
forced to change on a case-by-case basis), or sales and service
personnel might change (don't forget the former people as they often can
influence the latter).
It's an ever-shifting field.

3. returning to #1, you never know when your needs may change, so be
careful about swearing too much loyalty to a particular brand (unless
you have a very stable capital budget and senior management that trusts
you completely!).

4. I've known a lot of company staff from sales, service and/or R&D, and
have met relatively few 'duds'. These people work very hard and in a
surprising number of cases are paid even less than we are. For certain
they have nowhere near our degree of autonomy, so please keep their
names (pro or
con) off the air. I doubt any of us would appreciate our
qualifications and style being debated in public.

5. For that matter, why not simply keep specific product names off the
Listserver as much as possible? Give your candid impressions to the
inquirer off-line. If you feel the need to enlighten the Listserver at
large, confine your comments to more generic comments, "I find in my lab
hat a good X needs to have qualities A, B, C-- to get the job done".

6. As an example of #5, I have given some dozen workshops on hard
materials microtomy with both of the producers mentioned in the above
thread.
Students have attended who have, or are considering, instruments from
both vendors. The focus has religously been on the technique and its
potential results, not the pros and cons of the products that can get
you there, and the results have been very pleasing to all concerned. In
my opinion, this is the prime purpose of the Listserver; education, not
brand endorsement.

Tom

Dr. Tom Malis
Manager
Academic User Access Facility (AUAF)
CANMET Materials Technology Laboratory
Natural Resources Canada
558 Booth St., Ottawa, Ontario K1A 0G1
Tel.: (613) 995-7358 FAX: (613) 992-8735, cell: 613-371-4577
malis-at-nrcan.gc.ca {mailto:malis-at-nrcan.gc.ca}






From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 12:39:03 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 8 Apr 2005 11:10:09 -0700
Subject: [Microscopy] Re: TEM digital plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 8, 2005, at 7:58 AM, Linda Fox wrote:

} Is anyone using digital plates in place of film in their TEM's?
}
} Can you let me know about cost, efficiency, resolution, practicality,
} and any problems that you have had. Also what system you are using and
} has the service been good.
}
} We are looking into this option as one way to "go digital" with 2 old
} TEM's. Also, does anyone still use film, but then scan the images into
} a computer? That is another option that we are looking at.
}
Dear Linda,
Although I have not used imaging plates myself, I did investigate them
at one time. Their resolution and linearity are excellent, and their
quantum efficiency (the fraction of electrons that result in a signal)
is the best of any detector system I know of for ~100 kV electrons.
This falls off with increasing energy, however, and they are not so
good at 300-400 kV (let alone at 1.2 MV, which I was interested in).
I'm sure that the properties are likely to have improved since I looked
into things. The cost of the plates themselves is not an issue, since
they are reusable, and the cost of the reader was on the order of
$100,000. I expect that, if the readers have improved, the cost has
risen commensurably, but I am not up to date on this. As far as film
is concerned, we are still investigating whether film or CCD will
ultimately be better for single-particle analysis. The benefits of
film are the larger field of view and (for ED measurements) the fact
that one does not have to insert a beam stop over the unscattered beam.
The disadvantages of film are its limited linearity and the necessity
to scan it in order to use computer processing, either of which will
introduce errors into quantitation. It is also more difficult to get
the same consistency in the developing process as is inherent in a CCD
image. Additionally, to get good quantitation requires a good scanner,
and for ED, one needs something like the Perkin-Elmer microdensitometer
(at a cost of a few hundred thousand dollars), since one needs to get
accurate readings from small areas of high OD surrounded by large areas
of essentially transparent film. In other words, the best system
depends on just what kinds of experiments one is doing (and might be
doing in the future).
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 12:48:59 2005



From: Roger Baker :      rcbaker-at-eden.infohwy.com
Date: Fri, 8 Apr 2005 13:03:34 -0500
Subject: [Microscopy] Re: viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

One way to do the job is to paint two low resistance parallel lines on
the surface with a conductive silver particle-based paint (carbon based
conductive paint probably has too much resistance) and then use a good
digital ohmmeter to measure the resistance across the gap. The
resistance in of such an oxide film might be typically be on the order
of 10-100 ohms per square.

You might want to put a straight sliver of masking tape two
millimeters wide down first and then paint over it in a region 1 cm
long with the conductive paint. Since the painted conductors are 1 cm
long with a separation of 2 mm, this equals .2 square (once you peel
away the masking tape). You would then multiply the measured resistance
by five to get the correct value in ohms per square. -- Roger



}
} Email: mcary-at-gatan.com
} Name: Matthew Cary
}
} Organization: gatan inc
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: how do i most acuratly measure the ohms/square inch of an
} indium tin coated surface??
}
} -----------------------------------------------------------------------
} ----
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 13:18:24 2005



From: Scott Walck on Comcast :      swalck-at-comcast.net
Date: Fri, 8 Apr 2005 14:30:43 -0400
Subject: [Microscopy] viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sheet resistance is measured by a 4-point probe. The probes are equidistance
(about 1 mm) and inline. A constant current is put between the outer two
and the voltage drop between the inner two is measured. Then the sheet
resistance is given by V/I except that there is a geometric correction
factor (CF) to convert the voltage/current ratio measured by the 4-point
probe into sheet resistance. This correction factor accounts for the sample
size, shape and probe spacings. The probes are spring mounted to avoid
damaging the film. The sheet resistance measure by the probe is given by:

Rs = (V/I) * CF.

For an semi-infinite thin sheet, CF = 4.53. What is typically done is to
set the current at 0.453 mA and then multiply the voltage measured by 10.
The bulk resistivity of the coating material is given as Rs * t.

The measured resistance of a sample of dimensions L and W and sheet
resistance, Rs is given by
R = Rs * W/L. Which means that if you have a square sample of any size, the
resistance will be the same. An easy way to measure the sheet resistance
fairly accurately on transparent conducting coated glass samples is to take
a square sample of about 12 x 12 inches and measure the resistance with a
multimeter across it. If you know the sheet resistance of a coating, then
all you do is "count the squares". For example if you have a sample that is
twice its length, then the resistance along the long axis will be twice the
sheet resistance because it is two squares by one square, but across it, it
will be 1/2 because it is only a half square across.

There are commercially available four point probes for sheet resistance
available. I think that Keithley makes one. You might also consider this
source: http://bridgetec.com/srm.html
I Don't have any financial interest in either of these companies and I am
sure that they are not the only manufacturers of four point probes.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127





-----Original Message-----
} From: by way of MicroscopyListserver [mailto:mcary-at-gatan.com]
Sent: Friday, April 08, 2005 8:26 AM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mcary-at-gatan.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday,
April 7, 2005 at 12:12:38
---------------------------------------------------------------------------

Email: mcary-at-gatan.com
Name: Matthew Cary

Organization: gatan inc

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: how do i most acuratly measure the ohms/square inch of an indium
tin coated surface??

---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 13:35:30 2005



From: Valery Ray :      vray-at-partbeamsystech.com
Date: Fri, 8 Apr 2005 11:50:48 -0700 (PDT)
Subject: [Microscopy] Re: Re: viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Matthew,

In addition to the papers mentioned by Bill, you can
order a little book called "Low Level Measurements"
from Keithley Instruments, (www dot keithley dot com)
- it is a free literature.

On the pages 4-27 through 4-31 of this book is a
descripton of four-probe method for measurements of
surface resistivity, including some tricks for
enhancing accuracy.

Disclaimer: PBS&T has no financial interest in
Keithley Instruments, just a satisfied user.


--- Bill Tivol {tivol-at-caltech.edu} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
}
} On Apr 8, 2005, at 5:26 AM, by way of
} MicroscopyListserver wrote:
}
} } Question: how do i most acuratly measure the
} ohms/square inch of an
} } indium tin coated surface??
} }
} Dear Matthew,
} There are two papers by Mike Lamvik (RS Rader & MK
} Lamvik (1992)
} High-conductivity amorphous TiSi substrates for
} low-temperature
} electron microscopy. J. Microsc. 168, 71-77. and MK
} Lamvik, SD
} Davilla, and J Tuttle (1989) Properties of
} substrates for low
} temperature quantitative microscopy and
} microanalysis. Scan. Microsc.
} Suppl. 3 271-276.) that describe resistivity
} measurements of thin
} films. If you can configure your coating as
} described in those papers,
} that would be a way to measure the resistance
} accurately. If, however,
} your coating is already prepared, and if it is not
} on an insulating
} surface, you will have to go to greater lengths, and
} accurate
} measurement may not be possible. Good luck.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
}

Valery Ray

Particle Beam Systems
& Technology
www.partbeamsystech.com


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 14:15:15 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 08 Apr 2005 12:30:19 -0700
Subject: [Microscopy] Re: viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The "best" way to measure sheet resistivity is with a
four point probe using the van der Pauw method.
you can read about it here:

http://electron.mit.edu/~gsteele/vanderpauw/

The measured area must be on an insulator. Sheet rho is
in units of Ohms/square. However, if you did a square inch,
then you would have Ohms/square inch.

There are other structures that can be made that are still
four points. One such structure is a long (relatively) runner
with contacts at each end and two contacts placed inwards from
each end. Make the whole thing an nice number of squares (1x1u,
2x2u, 5x5u, etc.) and make the inner two taps at another nice
number of squares. Run current through the outer contacts and
measure the voltage across the inner contacts.

In all of these measurements, contact resistance must be kept
low or results are off.

gary g.



At 05:26 AM 4/8/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 14:25:22 2005



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 08 Apr 2005 17:46:57 -0400
Subject: [Microscopy] RE: re new anything, public critique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

Just a small correction, the secondary electrons from the backscattered
electrons coming back out of the sample are still being generated and
escaping close to the surface. It is the size of the interaction volume of
the primary electrons, loosing some energy deeper in and then returning
back towards the surface and generating secondaries there that decrease the
resolution. Because of the more forward scattering of electrons and
increased backscattered near the surface on tilted surface, that the
resolution is further degraded on surface not perpendicular to the beam.

With respect to heavier coatings, what is important is to match the coating
with the need and sample. If the bulk conductivity of the sample is so low
that it can trap charge within it, then a high accelerating voltage can
still charge a sample that has a heavy conductive coating because the charge
can't escape to the surface. What is best is to have a conductive coating
that is thin and uniform, i.e. continuous, across the surface and use a beam
energy that will allow the charge to drain from the surface. This requires
both rotating the sample and tilting it doing coating. Also remember that
even if you coat your sample, you still need for the charge to drain to
ground. The coating itself should not interfere with the imaging. The
material type and deposition method affects the grain size and if it is too
big for the magnification that you want to image, then that's what you are
going to see, not the sample.


-Scott

Disclaimer: South Bay Technology, Inc. manufacturers and sells the IBS/e
sputter coater for high resolution coatings.

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127


-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Friday, April 08, 2005 9:59 AM
To: Tay Yee Yan
Cc: microscopy-at-microscopy.com

Hi Yee Yan,

My understanding is that there are several factors involved in selecting
an accelerating voltage (KV). At higher KVs, chromatic aberration is
reduced, resulting in greater resolution, i.e., the ability to separate
two points visually. However, higher KVs also result in the generation
of secondary electrons from a larger surface area, due to primary beam
electron scattering over a larger lateral distance, as well as from
deeper within the specimen.

This theoretically has two effects. One is that the larger area of the
surface emitting SE's gives a larger "effective spot size", or area from
which signal is generated, and this decreases resolution. The other is
that the SEs escaping from deeper within the specimen are detected along
with the SEs generated at or just below the surface, and this part of
the signal can obscure surface detail by lowering contrast. This effect
can easily be seen by taking a biological specimen or other soft sample
and simply comparing a 1.0 KV image to a 30 KV image: the higher KV
image looks smoother and less detailed.

This effect is complicated by the effect of sample density, in that a
denser sample will have a smaller "effective spot size" than a
less-dense sample at the same KV. This means that for many samples you
can increase resolution by increasing KV if the sample is dense enough
to restrict the electron scattering to acceptable levels for the
magnification you are working at. In addition, higher KVs generally
allow such adjustments as using smaller apertures to decrease spherical
aberration effects and increase depth of field, and higher lens
currents to demagnify the probe size and increase resolution that way.
Generally speaking, adjusting the SEM for high resolution means
decreasing the signal to the detectors, so if you have more signal to
begin with, you can afford to lose more signal before noise overwhelms
your image.

I agree with you that for many samples, especially biological ones,
lower KVs are better at retaining surface detail in the image. We
routinely run our FESEM at 5.0 KV, and often at 1.0 KV. But not always.
Separating out the relative effects of larger "effective spot sizes"
(decreased resolution), escape of SE's from deeper within the specimen
(lower surface contrast), and decreased aberrations (increased
resolution) at high KV's is not straightforward. And let's not even get
into the effects of coatings on these factors, since you may need
heavier coatings to counter increased charging effects, as you
mentioned! As I said, it's probably best to approach each sample as
unique and experiment.

By copy of this to the listserv I am asking to be set straight on any
errors in the above sermon. I always learn a lot from these discussions
and have had faulty assumptions corrected on several occasions.

Cheers,
Randy

-----Original Message-----
} From: Tay Yee Yan [mailto:one_twinklestar-at-yahoo.com.sg]
Sent: Thursday, April 07, 2005 7:17 PM
To: Tindall, Randy D.

As someone who once made a very significant purchase from a major microscope
manufacturer and then received a condenser lens assembly that was missing an entire
lens element in its interior, only to encounter foot dragging and imperious
responses from the regional sales manager regarding getting it replaced, I think
there needs to be some room for factual critique that doesn't cross the bounds of
libel. Had I not been intimately familiar with the normal construction and
performance of the condenser, I would have a) suffered for ever after thinking that
we got what we paid for; or b) wasted days of my instititution's time getting to
the bottom of it. I don't know why U.S. researchers should feel the need to muzzle
themselves regarding any irresponsible behavior on the part of sales people or
unmet technical performance. After all, I would guess that a great many
microscopes are bought with tax dollars directly or indirectly.

John Twilley

Gerroir, Paul wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Tom,
} Well said. Unfortunately there have been individuals who use this
} listserver to make personal attacks publicly. Ultimately they are the
} ones who suffer.
}
} Paul
}
} Paul J. Gerroir
} Microscopy
} Materials Characterization
} Xerox Research Centre of Canada
} 2660 Speakman Drive
} Mississauga, Ontario L5K 2L1
}
} Phone: 905-823-7091, ext.216
} FAX: 905-822-7022
} e-mail: paul.gerroir-at-xrcc.xeroxlabs.com
}
} -----Original Message-----
} } From: Malis, Tom [mailto:malis-at-NRCan.gc.ca]
} Sent: Friday, April 08, 2005 10:25 AM
} To: 'microscopy-at-microscopy.com'
} Subject: [Microscopy] re new anything
}
} ------------------------------------------------------------------------
} ------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} -------
}
} I have been getting increasingly disturbed by the extensive traffic
} concerning both ultramicrotomes and personnel associated with companies
} selling the product. Some observations after roughly 30 years in the
} business of purchasing and maintaining both major instruments (TEM, SEM,
} XPS, Auger, EPMA, SIMS, etc) and ancillary equipment (microtomes,
} polishers, ion mills, etc):
}
} 1. for a given instrument most everyone with extensive experience
} possesses 'brand loyalty' to some degree, based on a combination of
} need,vs instrument specs, a good price, reasonable expectation of good
} service and user-friendlyness over a period of time.
}
} 2. even if the above has held true for some time, you never know when
} new developments may occur, pricing policies may change (or can be
} forced to change on a case-by-case basis), or sales and service
} personnel might change (don't forget the former people as they often can
} influence the latter).
} It's an ever-shifting field.
}
} 3. returning to #1, you never know when your needs may change, so be
} careful about swearing too much loyalty to a particular brand (unless
} you have a very stable capital budget and senior management that trusts
} you completely!).
}
} 4. I've known a lot of company staff from sales, service and/or R&D, and
} have met relatively few 'duds'. These people work very hard and in a
} surprising number of cases are paid even less than we are. For certain
} they have nowhere near our degree of autonomy, so please keep their
} names (pro or
} con) off the air. I doubt any of us would appreciate our
} qualifications and style being debated in public.
}
} 5. For that matter, why not simply keep specific product names off the
} Listserver as much as possible? Give your candid impressions to the
} inquirer off-line. If you feel the need to enlighten the Listserver at
} large, confine your comments to more generic comments, "I find in my lab
} hat a good X needs to have qualities A, B, C-- to get the job done".
}
} 6. As an example of #5, I have given some dozen workshops on hard
} materials microtomy with both of the producers mentioned in the above
} thread.
} Students have attended who have, or are considering, instruments from
} both vendors. The focus has religously been on the technique and its
} potential results, not the pros and cons of the products that can get
} you there, and the results have been very pleasing to all concerned. In
} my opinion, this is the prime purpose of the Listserver; education, not
} brand endorsement.
}
} Tom
}
} Dr. Tom Malis
} Manager
} Academic User Access Facility (AUAF)
} CANMET Materials Technology Laboratory
} Natural Resources Canada
} 558 Booth St., Ottawa, Ontario K1A 0G1
} Tel.: (613) 995-7358 FAX: (613) 992-8735, cell: 613-371-4577
} malis-at-nrcan.gc.ca {mailto:malis-at-nrcan.gc.ca}





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 17:13:48 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 8 Apr 2005 15:44:57 -0700
Subject: [Microscopy] Re: RE: re new anything, public critique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 8, 2005, at 2:46 PM, John Twilley wrote:

} As someone who once made a very significant purchase from a major
} microscope
} manufacturer and then received a condenser lens assembly that was
} missing an entire
} lens element in its interior, only to encounter foot dragging and
} imperious
} responses from the regional sales manager regarding getting it
} replaced, I think
} there needs to be some room for factual critique that doesn't cross
} the bounds of
} libel. Had I not been intimately familiar with the normal
} construction and
} performance of the condenser, I would have a) suffered for ever after
} thinking that
} we got what we paid for; or b) wasted days of my instititution's time
} getting to
} the bottom of it. I don't know why U.S. researchers should feel the
} need to muzzle
} themselves regarding any irresponsible behavior on the part of sales
} people or
} unmet technical performance. After all, I would guess that a great
} many
} microscopes are bought with tax dollars directly or indirectly.
}

} } Tom,
} } Well said. Unfortunately there have been individuals who use this
} } listserver to make personal attacks publicly. Ultimately they are the
} } ones who suffer.
} }
} } Paul


} } } 4. I've known a lot of company staff from sales, service and/or R&D,
} } } and
} } } have met relatively few 'duds'. These people work very hard and in a
} } } surprising number of cases are paid even less than we are. For
} } } certain
} } } they have nowhere near our degree of autonomy, so please keep their
} } } names (pro or
} } } con) off the air. I doubt any of us would appreciate our
} } } qualifications and style being debated in public.

Dear John,
Prompt, competent service is a key component of running any
complicated piece of equipment, so both positive and negative comments
regarding service experiences, IMHO, have a place on this list. These
can always be made without mentioning the name of the person involved,
and personal attacks--as opposed to opinions as to the level of
service--have no place here. Of course, the particularly competent
service or sales person would likely appreciate being mentioned by
name, and I think that there are circumstances where this is proper;
e.g., when someone either high up in a company or known to be in the
same area as the person who posted a question to the list has performed
particularly well. In that case, the poster would be likely to deal
with that same person.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 18:12:14 2005



From: Paul Webster :      pwebster-at-hei.org
Date: Fri, 08 Apr 2005 16:26:15 -0700
Subject: [Microscopy] Re: re new anything - long message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

First I would like to thank Tom Malis for bringing some fairness back onto
the listserver.

What we should be looking for here is educational material. If someone asks
for an opinion about what microtome to buy, then we can ask what
applications the machine is to be used for and what the prospective new
customer will expect of their machine. If the machine does not meet the
standards required of it, we should have enough knowledge to recognize this.

As an illustration, some years ago, I appealed to this list-server to help
me through a purchase of an SEM. As an electron microscopist who knew
nothing about SEM's, I mistakenly thought I would have some useful advice
given to me when I had to finally make a decision.

Instead I only received two, off-line replies that both basically told me to
buy one machine (both answers listed the same machine but neither said why I
should buy it). There was no reasoned discussion on-line about the different
features offered by the microscope companies and how they would be useful,
nor was there any advice on sputter coaters and other ancillary equipment.

Eventually I relied on books and advice from sales people to make my final
decision. It may not have been the best decision and I am sure that many
people would not agree with my choice but it fitted with what I needed at
the time. However, it would have been comforting to know a little more about
the differences (advantages/disadvantages) of cold cathode and Schottke
electron guns, or the usefulness of databases etc before purchasing.

As customers we need to know that machines will perform as they are
advertised. We also need to know what is essential on a machine and what we
can cut out if we have to cut costs. Also important is the quality of
service offered by a company both in skill and availability.

An an aside, I once had a service engineer tell me a machine wasn't working
because I didn't know how to use it properly. However, when I asked him to
show me, he admitted that he didn't know how to use it either. Should I have
expected him to know how to use it?

So, if anyone asks me for advice on which ultramicrotome to buy I will tell
them that all currently available machines will cut sections well - this is
true if the machine is made to specification. However, I also know that
older ultramicrotomes that have been around for many years also cut sections
well. My very first ultrathin cryosections were prepared using a Christensen
cryobox attached to a Sorvall MT2-B using a glass knife. The sections were
as good as any that can be made using new machines. However, these machines
are no-longer available and service is impossible.

Stretching the metaphor that was used in an earlier message, a Mercedes is
wonderful if you can afford to run it but why get one when it is only to be
used once a month to do the shopping? After all, if we all wanted "the best"
machines, surely we would all be using Macintosh computers.

More seriously, for the people wondering about which ultramicrotome to buy,
I would suggest first (if possible) taking a look at the machines at a trade
show. Don't expect the machine to be working, but you will get to see and
touch it. You will also meet some of the company people there who will give
you an insight on how the machine was developed and who uses it. Try to get
a list of customers from them and talk to the customers. Of course, most
customers will be partisan because they have to justify their purchase.
However, if you talk to enough people off the record, you will get a good
impression of the companies you will be dealing with.

The next step is to find a way to actually use a machine before you buy it.
Going to a working laboratory is the best way to do this so that you can see
how the machine performs in a real environment and see how the machine fits
your working methods. I once saw a well endowed lady try to use a machine
that required long arms to operate - it was obvious as soon as she sat down
in front of it that she would be unable to use it. This is something she
would not have discovered if she had to rely on the opinions of others.

If we assume that all machines will do the job they are supposed to do, then
the user interface is probably the most important part of any machine.
Evaluating the interface will be a personal process. For example, I think
that when I organize things everything is easily accessible. My staff, who
are all significantly shorter than me, think the place is a disaster when I
put things away because they can't reach most of the stuff. Of course, I
don't spend all my time in the lab so they are correct in their assessment.

Another good way of evaluating a machine, and especially an ultramicrotome,
is to use it on a teaching course. A three day workshop will reveal much
about how a machine works. Watching machines working for 10 days, being
operated by novices is especially interesting.

Marc Pypaert, in his message, mentions an EMBO course in Paris last year
where cryoultramicrotomes were installed in a crowded laboratory and left
working all day for 10 days. This is an extreme case of what we would expect
a machine to endure yet most of the machines endured it. I am sure that many
sales were made as a result of what the participants witnessed. However,
there have been many other courses where some machines have failed and
others have excelled. Such endurance cannot be the only way of evaluating a
machine. Remember that most courses are held away from the home base of the
manufacturer and machines have to be shipped in (at great cost) for use on
the course. Damning a machine for not working under such conditions is
unfair.

In conclusion, the sale of ultramicrotomes has become a hot topic and the
support for different companies is probably more political than it should
be. As Tom Malis says, the people involved with manufacture and sales are
people too and are trying to do their job. Sometimes what they want to do
and what they have to do are conflicting tasks. However, if service and
machines are substandard the company will suffer in the long term. This is
normal in the business environment.

We can also affect the way we are treated by manufacturing and sales people
by the way we interact with them. This too is normal because we are all
human.

Our job as customers is to find what works best for us using as much
knowledge as we can obtain. As advisors our role is to educate the
customers.

If you read this far, thank you for your time.

Regards,

Paul Webster.



Paul Webster, Ph.D.
House Ear Institute
2100 West Third Street
Los Angeles
CA 90057-1922
Phone: 213 273 8026
Fax: 213 13 739






On 4/8/05 7:24 AM, "Malis, Tom" {malis-at-nrcan.gc.ca} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} I have been getting increasingly disturbed by the extensive traffic
} concerning both ultramicrotomes and personnel associated with companies
} selling the product. Some observations after roughly 30 years in the
} business of purchasing and maintaining both major instruments (TEM, SEM,
} XPS, Auger, EPMA, SIMS, etc) and ancillary equipment (microtomes, polishers,
} ion mills, etc):
}
} 1. for a given instrument most everyone with extensive experience possesses
} 'brand loyalty' to some degree, based on a combination of need,vs instrument
} specs, a good price, reasonable expectation of good service and
} user-friendlyness over a period of time.
}
} 2. even if the above has held true for some time, you never know when new
} developments may occur, pricing policies may change (or can be forced to
} change on a case-by-case basis), or sales and service personnel might change
} (don't forget the former people as they often can influence the latter).
} It's an ever-shifting field.
}
} 3. returning to #1, you never know when your needs may change, so be careful
} about swearing too much loyalty to a particular brand (unless you have a
} very stable capital budget and senior management that trusts you
} completely!).
}
} 4. I've known a lot of company staff from sales, service and/or R&D, and
} have met relatively few 'duds'. These people work very hard and in a
} surprising number of cases are paid even less than we are. For certain they
} have nowhere near our degree of autonomy, so please keep their names (pro or
} con) off the air. I doubt any of us would appreciate our qualifications
} and style being debated in public.
}
} 5. For that matter, why not simply keep specific product names off the
} Listserver as much as possible? Give your candid impressions to the
} inquirer off-line. If you feel the need to enlighten the Listserver at
} large, confine your comments to more generic comments, "I find in my lab hat
} a good X needs to have qualities A, B, C-- to get the job done".
}
} 6. As an example of #5, I have given some dozen workshops on hard materials
} microtomy with both of the producers mentioned in the above thread.
} Students have attended who have, or are considering, instruments from both
} vendors. The focus has religously been on the technique and its potential
} results, not the pros and cons of the products that can get you there, and
} the results have been very pleasing to all concerned. In my opinion, this
} is the prime purpose of the Listserver; education, not brand endorsement.
}
} Tom
}
} Dr. Tom Malis
} Manager
} Academic User Access Facility (AUAF)
} CANMET Materials Technology Laboratory
} Natural Resources Canada
} 558 Booth St., Ottawa, Ontario K1A 0G1
} Tel.: (613) 995-7358 FAX: (613) 992-8735, cell: 613-371-4577
} malis-at-nrcan.gc.ca {mailto:malis-at-nrcan.gc.ca}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 18:13:38 2005



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Fri, 8 Apr 2005 19:28:09 -0700
Subject: [Microscopy] Fwd: Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Debby and LISTers
OOPS! I apologize. I thought you were asking for a TEM protocol - I
also have one for SEM. There is no need to reply. I shall FAX it to
you.
C.

} X-Authentication-Warning: ns.microscopy.com: mail set sender to
} MicroscopyL-request-at-ns.microscopy.com using -f
} X-Sender: heckman-at-mailstore.bgsu.edu
} Date: Thu, 7 Apr 2005 12:52:07 -0700
} To: {microscopy-at-MSA.microscopy.com} , Debby Sherman {dsherman-at-purdue.edu}
} From: Carol Heckman {heckman-at-bgnet.bgsu.edu}
} Subject: [Microscopy] Re: Cultured cell prep for SEM
} X-MASF: 0.00%
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu
http://www.bgsu.edu/departments/biology/facilities/MnM
___________________________________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 23:58:09 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 8 Apr 2005 22:12:58 -0700
Subject: [Microscopy] RE: Re: re new anything - long message (short response, though)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Uh, guys, well, thank you all for your opinions on how this listserver
ought to be run and what is appropriate to be on it, but ah, isn't all
that really up to Nestor, since he runs it (a great personal effort
perhaps not always sufficiently appreciated)?

John Mardinly
408-765-2346
877-277-1182





From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 00:30:33 2005



From: Alberto Diaspro :      diaspro-at-fisica.unige.it
Date: Sat, 9 Apr 2005 07:44:59 +0200
Subject: [Microscopy] reprint about Abbe's work et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear friends,
I'd appreciate very much if you you send me the pdf of the following
paper:
Applied Optics, Volume 5, Issue 11, 1720-
November 1966

Ernst Abbe and his work

H. Volkmann

and some other pdf or ppt related to Abbe's formula. IN Genoa, within
the Science Festival 2005, I am tentatively organizing an exhibition of
microscopes dedited to Abbe's work, celebrating again 1905-2005.
Thank you in advance for your help.
All my best
Alby

p.s. Science festival will be held in Genoa from October 25 to
Novembere 5, approx, see also at
http://www.festival.infm.it/it/home.php

------------------------------------------------------------------------
------------------------------------------------------------------------
--------------------------------------------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)
------------------------------------------------------------------------
------------------------------------------------------------------------
-------------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department
of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309; URL:
http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 08:32:10 2005



From: donc-at-asmicro.com (by way of MicroscopyListserver)
Date: Sat, 9 Apr 2005 08:48:15 -0500
Subject: [Microscopy] viaWWW: AFM - want used Dimension scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (donc-at-asmicro.com) from on Friday, April 8, 2005 at 14:30:20
---------------------------------------------------------------------------

Email: donc-at-asmicro.com
Name: Don Chernoff

Organization: Advanced Surface Microscopy Inc

Title-Subject: [Microscopy] [Filtered] MListserver: AFM - want used Dimension scanner

Question: Where can I buy a used Dimension AFM scanner?


If you are not familiar with it, this is a component of Dimension-series (3000, 3100, 5000, etc...) large sample AFMs sold under the NanoScope brand by Veeco Metrology/Digital Instruments.
Identifying photos are shown at:
http://www.asmicro.com/Equipment/Identifying_NanoScope.htm

Please reply directly to me:
donc-at-asmicro.com

regards,
Don Chernoff
{business activities: analytical services in AFM, AFM probes, calibration and test specimens, calibration and measurement software, used NanoScope equipment.}
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd. Suite 120 Voice: 317-895-5630
INDIANAPOLIS IN 46220 Toll free: 800-374-8557 (in USA)
web: http://www.asmicro.com Fax: 317-895-5652


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 17:54:18 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Sat, 09 Apr 2005 16:09:17 -0700
Subject: [Microscopy] Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Epson 4870 does an excellent job for all negs 4 x 5 and smaller.
One can also use their 3200 Scanner which is a bit cheaper. It doesn't
scan as many negs at once as the 4870, but works fine.

Some of the lower end Microtech scanners also do a decent job.

Judy


Judy Murphy, PhD
Microscopy Training, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net


Garry Burgess wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 17:56:48 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Sat, 09 Apr 2005 16:12:03 -0700
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

How about Two Beam (not very creative I guess, but perhaps workable)

Judy


Judy Murphy, PhD
Microscopy Training, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net


Richard Edelmann wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 18:12:44 2005



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Sat, 9 Apr 2005 13:28:29 -1000 (HST)
Subject: [Microscopy] Tips for M&M05 in Hawaii

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello, All-

I've lived in Hawaii for just short of 37 years and, like the native New
Yorker who has never been to the Statue of Liberty, I'm not a good tour
guide for HOnolulu in its current incarnation. But I'm getting daily
emails from microscopists about Microscopy & Microanalysis 2005 here in
Honolulu, asking about hotels and transportation, so here are some
observations that might help you.

Waikiki is about a mile end to end, and the Honolulu Convention Center is
at the far western end, actually a bit beyond Waikiki proper, and not next
to the bulk of the hotels. Waikiki is flat and an easy walk. The three
meeting hotels are about 20 and 25 minutes' walk from the Convention
Center, and both the Sheraton Waikiki and the Hyatt Regency Waikiki will
have shuttle buses to the Convention Center for meeting attendees. Meeting
rates for both hotels are very good compared to their published
prices. Both larger hotels have lots of amenities and seasonal children's
programs (better check first), and I think that people who choose the
Sheraton Princess Kaiulani have privileges at the other Sheratons as well.

For those who choose not to stay in the above hotels, you might find some
budget hotels, especially those away from the beach, such as along the Ala
Wai Canal. Be aware that whole blocks at the western end of Waikiki are
about to be torn down for a major new development. If you are nostalgic
about rooms and eateries along part of Lewers (the makai, or ocean end),
Beachwalk, Saratoga and Kalia, they will probably be a big hole in the
ground in August! This will also put pressure on hotel rooms, so please
book soon if you have not already. There are very few hotels outside of
Waikiki, except for a few high-end resorts. If you are looking for cheap
accommodations, be aware that you will be competing with lots of others
who are relatively poor, such as the nearly homeless and drug-addicted.
Waikiki is currently pretty safe, but you will also find those elements
there. It's a big city and resort area with the usual problems; please
exercise good judgment!

Traffic is really heavy in Waikiki and around the Convention Center, and
parking is at a premium. I personally recommend that you do not have a car
for the meeting portion of your stay, and then rent a car for sightseeing
before or after the meeting. To get to the Convention Center by city bus
(TheBus) from Waikiki, take the #2 or #13 bus (westbound) on Kuhio
Ave. Get off on the corner of Kalakaua Ave. and Kapiolani Blvd. in front
of Hard Rock Cafe, then cross the street (scary intersection!) to the
Convention Center. TheBus (http://www.thebus.org, $2 per trip) is a pretty
good way to get around island-wide. If you have family accompanying you,
they will find lots to do within Waikiki or on tours, easily booked in the
lobby of most hotels, and they may be able to do without a car until you
can join them.

There is a recently published book that is pretty thorough, Oahu
Revealed; the Ultimate Guide to Honolulu, Waikiki & Beyond by Andrew
Doughty and Harriett Friedman. It has good maps, hotel and food reviews
and tips, and I recommend it. The authors have also covered Maui, Kauai
and the Big Island, and those books are supposed to be excellent.

I think airfares just hopped up, but most people I've talked to have been
able to find some pretty good deals. You might look for fly/room/car deals
and deals that combine inter-island travel as well. Travel agents often
have access to excursion rates. Take heart in knowing that you will be
able to get to Hawaii and back cheaper than I can get to the Mainland and
back! I find Microscopy & Microanalysis worth the expense each year,
anyway. With an excellent program and over 1100 papers, this will be the
biggest M&M ever! And you won't want to miss the usual fabulous trade
show. I hope to see all of you in a few months.

Aloha,
Tina

Microscopy & Microanalysis 2005
Local Arrangements Committee Co-Chair

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From MicroscopyL-request-at-ns.microscopy.com Sun Apr 10 11:24:13 2005



From: somayyeh_kheiri-at-yahoo.com (by way of Ask-A-Microscopist)
Date: Sun, 10 Apr 2005 11:43:25 -0500
Subject: [Microscopy] AskAMicroscopist: counting of pollen grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, April 10, 2005 at 10:55:17
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh

Organization: kheiri

Education: Graduate College

Location: urmia,Iran

Question: I have been counting the number of pollen grains and ovules to get the
pollen/ovule ratio for verbascum species
and populations.

I pressed the anthers of the flowers and provided a susponsion of the
debris of anthers in distilled water to count the pollen
grains in a 5 microliter volume.

some of the samples in the susponsion were counted
one day after they were provided so the result showed
differences from the first sample which was counted immidiately after
providing the susponsion.
I myself thought keeping the material in water for oneday
caused the grains to be attached together and the amount of the grains
that are counted are reduced.

can anybody help me solve the problem?

is this reasonable or there maybe errors in the method of counting?

I appreciate any kind reply.

Somayyeh


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 08:04:16 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 11 Apr 2005 09:22:27 -0400
Subject: [Microscopy] Re: Tips for M&M05 in Hawaii

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Tina,
Thank you for the advice. See you in August.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 08:21:57 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Mon, 11 Apr 2005 08:41:28 -0500
Subject: [Microscopy] Administrivia: March Archives On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues....


The March Archives are now available at:

http://www.microscopy.com/MicroscopyListserver


Nestor
Your FriendlyNeighborhood SysOp


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 08:56:50 2005



From: Lou Ross :      RossLM-at-missouri.edu
Date: Mon, 11 Apr 2005 09:15:29 -0500
Subject: [Microscopy] 3rd annual Short Course on Computer-Assisted Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Electron Microscopy Core Facility at the University of
Missouri-Columbia is hosting the 3rd annual Short Course and Workshop
on Computer-Assisted Image Analysis and Measurement taught by Dr.
John C. Russ on May 25-27, 2005. This popular course is intended to
familiarize users of image analysis equipment with the fundamental
principles and methods available to obtain meaningful results, and to
educate laboratory supervisors or research professionals seeking to
learn how to use such methods in their applications. The techniques
are applicable to fields ranging from materials, geological and
biological/medical research to food technology and manufacturing
quality control.

The course relies heavily on tightly coupled lectures and hands-on
experience with the various techniques. The laboratory includes a
wide variety of image analysis methods designed to cover the range of
approaches and tools, and a detailed set of practical instructions to
enable their use with a minimal learning curve. No specific
background is assumed, although users should already be familiar with
microscopy or other imaging technology, and the techniques required
to obtain the images to be measured. Many of the examples used in the
course involve light or electron microscope images, but students are
invited to bring their own most interesting images (TIFF files) for
discussion and analysis.

Image analysis and measurement methods are used in a broad range of
applications and are usually concerned with extracting a few
numerical values, such as the number, size, shape or location of
objects from the image. In other cases, global structural parameters
such as measures of the volume and surface of structures present are
of interest. These measurements may require image processing to
correct defects, feature enhancement, comparison of multiple images,
object recognition, or other steps. Ultimately, the image is reduced
to just the features of interest. Measurements on these individual
features, or on the image as a whole, must then be obtained and
interpreted in a proper stereological context to obtain useful data
about the objects. Statistical interpretation of the data allows
comparisons of different populations, understanding of distribution
plots, and other inferences about the original objects. Structural
modeling and geometric probability can be used to develop models for
this interpretation.

Dr. Russ is the internationally acclaimed author of innumerable
articles and several books on image analysis techniques and digital
imaging, including the The Image Processing Handbook. He is widely
known for his workshops and short courses and has helped to develop
software packages to make image analysis methods more accessible to
non-specialists.

The registration fee is $1100 and has an enrollment limit of 20.
Registration deadline is April 29. More information can be found at
{http://www.emc.missouri.edu/works.htm} http://www.emc.missouri.edu/works.htm,
or by contacting course coordinator Lou Ross at 573.882.4777 or
rosslm-at-missouri.edu.

Lou Ross
--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 10:34:48 2005



From: John Shields :      jpshield-at-uga.edu
Date: Mon, 11 Apr 2005 11:53:42 -0400
Subject: [Microscopy] SEMS meeting in Pensacola

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Just a reminder...
Greetings Members,

The Southeastern Microscopy Society's 41st Annual Meeting is
fast approaching us. The Meeting Dates are May 18-20, 2005
and will be held at the Hilton Garden Inn, Pensacola Beach,
Florida.

Meeting Information can be found on the SEMS Website at:
http://www.semicroscopy.org/

As a reminder, please note the following deadlines and
Abstract Due Date Extension:*

*1. Deadline for receipt of abstracts (Including Ruska Award
Participants) was April 10, 2005 to Jim Sheetz, (see address
below).
THE DEADLINE FOR ABSTRACT SUBMISSIONS HAS BEEN EXTENDED TO
APRIL 18!!!!

Abstract Submissions to BOTH:
Dr. Jim Sheetz
Dept. Cell Biology
Vocker Hall, Box 302
Univ. of Alabama at Birmingham
1670 Univ. Blvd.
Birmingham, AL 35294
and
Dr. John P. Shields phone:706-542-4080
EM Lab e-mail: jshields-at-cb.uga.edu
151 Barrow HallUniversity of Georgia

Questions or problems with preparation of abstracts - please
contact the Proceedings Editor (John
Shields: phone: 706-542-4080 or e-mail: jshields-at-cb.uga.edu.

2. Hotel Reservation Deadline: April 17, 2005

Please note that reservations made after April 17 will be
subject to rates based on availability at the time the
reservations are made. After April 17, please continue to
ask for "Group Reservations" when calling the Hilton and use
Group/Convention code SMS so that SEMS will be credited.

Property Location and Reservation Information
Hilton Garden Inn
12 Via de Luna Drive
Pensacola Beach FL 32561
1-866-916-2999 Ask for "Group Reservations"
3. Conference Registration Fee(s) Deadline: Early
Registration APRIL 25
To register, please mail form to:
Ms. Karen L. Kelley Phone: (352)-392-
1184
University of Florida Fax: (352)-846-
0251
P.O. Box 118525
Gainesville, Florida, 32611
John P. Shields
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602

706-542-4080


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 13:13:13 2005



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 9 Apr 2005 12:07:12 +0100
Subject: [Microscopy] Re: RE: HR images-Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi All

[This note is with the blessing of Randy Tindall]

I think we have a bit of a problem with Randy's explanation of secondary
electron signals?

} My understanding is that there are several factors involved in selecting
} an accelerating voltage (KV). At higher KVs, chromatic aberration is
} reduced, resulting in greater resolution, i.e., the ability to separate
} two points visually. However, higher KVs also result in the generation
} of secondary electrons from a larger surface area, due to primary beam
} electron scattering over a larger lateral distance, as well as from
} deeper within the specimen

I have to dispute the second sentence. Secondary electrons have an energy
of less than 50eV be they produced from a 500v beam or a 1MeV beam. Thus
their mean free path within the specimen is reduced to less than 15nm!
David Joy explains that 80% of the SE signal comes from the top 5nm, around
15% from the next 5nm and less than 5% from the final 5nm.

} This theoretically has two effects. One is that the larger area of the
} surface emitting SE's gives a larger "effective spot size", or area from
} which signal is generated, and this decreases resolution. The other is
} that the SEs escaping from deeper within the specimen are detected along
} with the SEs generated at or just below the surface, and this part of
} the signal can obscure surface detail by lowering contrast. This effect
} can easily be seen by taking a biological specimen or other soft sample
} and simply comparing a 1.0 KV image to a 30 KV image: the higher KV
} image looks smoother and less detailed.

Higher kV actually produce a probe nearer to the theoretical probe size than
do lower kV where aberrations and external fields play their part in
disturbing the beam. I would agree that the higher the kV the higher the
emission of backscattered electrons and these are dependant upon incident
beam energy. In light element materials like biological specimens a 30kV
beam may bring backscattered information from as much as 3um below the
surface and it is this information that may blur the image and spoil
resolution. BSE you see come from the full width of the reaction volume and
are therefore at a much lower spatial resolution. It is BSE not SE that are
spoiling images. If you increase the kV and the specimen becomes more
interesting it is the sub surface detail (BSE) that is bringing the interest
as the BSE overwhelm the SE. Lowering the kV the number of BSE are reduced
and the SE start to dominate. In a material science specimens the sub
surface detail may be of more interest than the surface; cracks, porosity
and different phases with different density etc etc.

} This effect is complicated by the effect of sample density, in that a
} denser sample will have a smaller "effective spot size" than a
} less-dense sample at the same KV. This means that for many samples you
} can increase resolution by increasing KV if the sample is dense enough
} to restrict the electron scattering to acceptable levels for the
} magnification you are working at. In addition, higher KVs generally
} allow such adjustments as using smaller apertures to decrease spherical
} aberration effects and increase depth of field, and higher lens
} currents to demagnify the probe size and increase resolution that way.
} Generally speaking, adjusting the SEM for high resolution means
} decreasing the signal to the detectors, so if you have more signal to
} begin with, you can afford to lose more signal before noise overwhelms
} your image.

The biggest gain in performance is to optimise the kV for the task in hand.
Balancing resolution with information is more important than out and out
resolution even when working a FEG system at 350,000X! As a general
statement biological specimens will be best between 2 and 10kV, other light
elements (Al, Si) 5 to 10kV, nickel, iron and copper alloys 7 to 15kV. The
performance of a FEG SEM like any other often depends upon emission current
rather than kV. Optimise the kV for the specimen then tune the emission
current to optimise performance and signal when apertures and spot sizes are
brought into play. It may interest you to know that working with an Intel
laboratory we found on a Hitachi S4500 we only needed spot size 8 to achieve
the highest resolution working at 4mm 15kV. Smaller spot sizes in a FEG SEM
usually do not have the same advantage as in a tungsten hairpin instrument.
Short working distances, good alignment of the gun and top notch alignment
for the aperture (up to the magnification you intend to record the image)
are far more important than putting effort into "reducing aberrations" by
other means.

} I agree with you that for many samples, especially biological ones,
} lower KVs are better at retaining surface detail in the image. We
} routinely run our FESEM at 5.0 KV, and often at 1.0 KV. But not always.
} Separating out the relative effects of larger "effective spot sizes"
} (decreased resolution), escape of SE's from deeper within the specimen
} (lower surface contrast), and decreased aberrations (increased
} resolution) at high KV's is not straightforward. And let's not even get
} into the effects of coatings on these factors, since you may need
} heavier coatings to counter increased charging effects, as you
} mentioned! As I said, it's probably best to approach each sample as
} unique and experiment.

Please forget about SE from greater depths and concentrate on balancing the
information you require. Very short WD ( {5) on a 4500/4700 restricts the
imaging data to SE which can be very boring and this restriction vastly
increases the possibility of charge. Move away from the lens a little (~7
to 9mm) and you bring in some of the BSE information that has been converted
to secondaries, these will provide added contrast, some image depth and most
of all less charge.

} By copy of this to the listserv I am asking to be set straight on any
} errors in the above sermon. I always learn a lot from these discussions
} and have had faulty assumptions corrected on several occasions.

I have a high regard for Randy hence this reply is not going to the
listserver unless he requests it? I do not remember if I gave Randy a copy
of the "Working With a SEM CD" where you will find a full explanation of
signals. If I have time I will put something on my web site in Hints and
Tips - Monday.

Regards to you all

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
www.emcourses.com tel +44 1280 816512 fax +44 1280 814007



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 15:02:07 2005



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 11 Apr 2005 10:20:58 -1000 (HST)
Subject: [Microscopy] Top 10 Reasons to Attend M&M 2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Top 10 Reasons your Boss Should Send You to M&M 2005 in Honolulu

1. You'll be networking with colleagues from the 7 participating
microscopy societies, making great international contacts and developing
new scientific collaborations

2. You'll see the latest and greatest in instrumentation and techniques
at the M&M exhibit

3. Pre-meeting congress and short courses followed by a compelling
scientific program of over 1100 papers from 40 different countries will
prevent you from getting a sunburn

4. Vendor and sponsor tutorials in new and emerging technologies will
keep you up-to-date

5. Free popcorn and beer in the exhibit hall/poster sessions will keep
you off the beach

6. You have a chance to win a diamond knife and other goodies for your
lab

7. You can interact with vendors so that they know what you need in a
product

8. Face-to-face problem solving with colleagues and experts in the field

9. With the time zone difference you can get most of a day's work done by
internet before even going to the meeting

10. You promise to teach everyone the hula when you return



Keep up with the news on the meeting website
http://mm2005/microscopy.org




****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 15:53:09 2005



From: Scott Griffith :      skod-at-ises-llc.com
Date: Mon, 11 Apr 2005 15:12:08 -0600
Subject: [Microscopy] Searching for Leitz Ergolux AMC documentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sirs:

I'm a new list member who would like to contact anyone on the list who
might have access to any documentation for an early 90's Leitz Ergolux AMC
optical microscrope, particularly with respect to the optional laser
autofocus hardware and stage controller. I recently acquired one in quite
good condition at an auction, and I am slowly bringing it up to replace my
venerable old Orthoplan with its time-consuming manual stage and focus.
Having this machine up and running will be a tremendous boon to my
business.

The microscope is part number 020-501.015, the laser autofocus module on
the illuminator path is 036-094.102, dated January 1991. The stage is
labelled Wild Leitz 026-407.110, but looks identical to a number of
Marzhauser stages I've dealt with in the past (rectangular motor cases
with no manual knobs). The joystick/controller pad is one of the older
black Leitz units with a 2-line LCD readout and a DB25 interface to the
electronics rack, numbered 301-336.029. And finally, the electronics rack
for the unit that contains the autofocus controller, stage motor drivers,
IEE 488 interface, video hardware, and other ancillary gear is numbered
301-341.150.

Regrettably, Leitz USA has turned out to be essentially no help on this,
and the instrument appears to be more or less completely unsupported.
Therefore, if anyone has some of this ancient documentation gathering dust
on the shelves in the back room (particularly the command set for stage
positioning, and any schematics or the like), please contact me offlist at
the email address below to discuss them. I'd ask that we take this
discussion offlist to preserve listserver bandwidth- because wrenching on
this poor old beast is probably not of general interest!

Thanks very much in advance for any help any of you might be able to
render. I'd be eternally grateful for any feedback on this query.

Yours truly,

Scott Griffith, ISES-LLC
9745 Steeplechase Drive
Franktown, CO 80116
303-660-2541 voice
303-660-2542 fax
skod-at-ises-llc.com


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 18:28:01 2005



From: Ben Moshiri :      Ben.Moshiri-at-ametek.com
Date: Mon, 11 Apr 2005 19:47:00 -0400
Subject: [Microscopy] Job Opening - Crystallography (EBSD) Product Manager

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Product Manager, Crystallography Products
EDAX, Mahwah, NJ, USA

Description:
Management of Crystallography (Electron Backscatter Diffraction) products,
including definition of future products, communicating customer/market
requirements to the rest of the organization, executing marketing programs
and providing sales support.

Responsibilities:
Develop product line concepts and position the product line in the
marketplace. Analyze the marketplace to determine growth and technology
trends.
Develop short, medium and long term plans and forecasts for the product
line.
Work closely with other Product Managers to ensure continuity and
coherence in the marketing, design and support of integrated products.

Qualifications and Requirements:
BS or MS in Science/Engineering.
MBA or equivalent highly desirable.
5 years of relevant work experience in a marketing or sales environment.
Previous Product Management experience highly desirable.
Technically knowledgeable about elemental analysis and electron
microscopy.
Excellent communication/interpersonal skills and project management
abilities.

The Company:
EDAX is a leader in EDS, EBSD and Micro-XRF systems, serving customers
across the globe. EDAX is a unit of AMETEK (NYSE: AME), a global
manufacturer of electronic instruments with annual sales of over $1.2
billion.

An excellent compensation package is offered, together with a relocation
package.

Contact Information:
Roberta Burns, HR Manager
Roberta.Burns-at-ametek.com
+1 201 529 4880
www.edax.com




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 10:52:11 2005



From: Mark Robertson-Tessi :      mtessi20-at-yahoo.com
Date: Tue, 12 Apr 2005 09:11:02 -0700 (PDT)
Subject: [Microscopy] Re: HR images-Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

--- Steve Chapman {protrain-at-emcourses.com} wrote:

} Very short WD ( {5) on a 4500/4700 restricts the
} imaging data to SE which can be very boring and this restriction vastly
} increases the possibility of charge. Move away from the lens a little (~7
} to 9mm) and you bring in some of the BSE information that has been converted
} to secondaries, these will provide added contrast, some image depth and most
} of all less charge.

Steve,

I do not see why changing the working distance will reduce charging, all other
things being equal. Whether or not signal is reaches a detector does not
affect charging, I thought. Please explain.

Cheers,
Mark Robertson-Tessi





__________________________________
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
http://smallbusiness.yahoo.com/resources/


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 13:50:31 2005



From: Chaoying Ni :      cni-at-UDel.Edu
Date: Tue, 12 Apr 2005 15:08:46 -0400 (EDT)
Subject: [Microscopy] Re: Re: HR images-Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Mark,

WD or the geometry between a detector and sample surface does affect the
S/N, especially the SE flux to the detector, hence the contrast of an
image. Charging electrons are primarily those having energies ~10keV or
less (SE counts vs. keV curve). By increasing WD, some of those electrons
may not survive the distance, so less charging is visible. To minimize
charging effects some vendors have designs to filter/block electrons
within a keV window from getting into detectors.

In a sense, you are right, SE electrons of varied energy are produced
anyway with little dependence on WD. But as long as "the charging
electrons" dont get into the detector and dont interfere with the incident
beam, they are not visible.

****************************************
Chaoying Ni, PhD
The W.M. Keck Electron Microscope Facility
Facility location: 022 Spencer Laboratory
Mailing address:
201 duPont Hall
Dept of Matls Sci & Eng
University of Delaware
Newark, DE 19716
(302) 831-2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************


On Tue, 12 Apr 2005, Mark Robertson-Tessi wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} --- Steve Chapman {protrain-at-emcourses.com} wrote:
}
} } Very short WD ( {5) on a 4500/4700 restricts the
} } imaging data to SE which can be very boring and this restriction vastly
} } increases the possibility of charge. Move away from the lens a little (~7
} } to 9mm) and you bring in some of the BSE information that has been converted
} } to secondaries, these will provide added contrast, some image depth and most
} } of all less charge.
}
} Steve,
}
} I do not see why changing the working distance will reduce charging, all other
} things being equal. Whether or not signal is reaches a detector does not
} affect charging, I thought. Please explain.
}
} Cheers,
} Mark Robertson-Tessi
}
}
}
}
}
} __________________________________
} Do you Yahoo!?
} Yahoo! Small Business - Try our new resources site!
} http://smallbusiness.yahoo.com/resources/
}
}


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 15:03:11 2005



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Tue, 12 Apr 2005 21:17:34 +0100
Subject: [Microscopy] Re: Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Perhaps I didn't make myself clear.

I am not objecting to trademarks per se. If somebody has a
distinguishing trademark ("Tecnai" or "Gemini", are obvious examples
in the field of electron microscopy), then I'm all in favour of
providing legal protection to that distiguishing mark.

What I am suggesting is that to trademark simply descriptive terms is
a nonsense. Trademarking, in my personal opinion, of "Dualbeam" or
"Crossbeam" is equivalent to trademarking "Mass Spectrometer" or
"Yellow". It achieves little for the companies concerned, other than
giving companies lawyers work and suggests that the marketing is
being run by some rather unimaginative people. It certainly fails to
generate a distintive brand identity.
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 15:35:13 2005



From: Becky Holdford :      r-holdford-at-ti.com
Date: Tue, 12 Apr 2005 15:53:25 -0500
Subject: [Microscopy] Re: Re: Trade names: was: "Dualbeam" "Crossbeam"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Richard: as a long-time user of these types of tools, I usually refer to
them as "dual-column" FIBs, unless I'm actually referencing a particular
model.

Richard Edelmann wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 15:47:42 2005



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Tue, 12 Apr 2005 17:05:51 -0400
Subject: [Microscopy] Re: RE: HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm not sure if this is pertinent but check your file parameters when saving
images. These problems do not occur on my system if I save images in TIFF
or JPEG formats. I've only observed this when saving them as Bitmap. I
personally recommend using the TIFF format.

If you are using the Hitachi data manager you select the format upon saving
the image.

If you are using PCI Quartz you need to change this in the PCI.INI file
located on the local PC. Open the PCI.INI file in notepad and make an edit
to the "ImageFileType=" line as shown below:


[Files]
ImageFileType=.TIF
ImageDirectory=
ImportDirectory=C:\aaaacalibration
PhotoCDPage=0

Then save the file. When PCI Quartz is launched it will save the images as
TIF files. I hope this helps?

John A. Robson
Boehringer Ingelheim Pharmaceuticals, Inc.
PO Box 368
900 Ridgebury Rd
Ridgefield, CT 06877

Phone (203)798-5640
Fax (203)798-5698
e-mail jrobson-at-RDG.boehringer-ingelheim.com



-----Original Message-----
} From: bbandli [mailto:bbandli-at-mvainc.com]
Sent: Thursday, April 07, 2005 2:45 PM
To: microscopy-at-microscopy.com

Our JEOL6500-F (JEOL PCSEM software) has the same indexed color
"feature" and converting it to 8-bit greyscale has the same effect of
cleaning up the noise in the image. I also have no idea why.

Bryan Bandli

Tindall, Randy D. wrote:

} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 21:34:41 2005



From: Taylor, Samantha :      Samantha.Taylor-at-alcoa.com.au
Date: Wed, 13 Apr 2005 10:52:40 +0800
Subject: [Microscopy] FEI Quanta 400 FEG

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi All

I am looking for information on the FEI Quanta 400 FEG. If you have one
of these instruments - can you please let me know what you think.

Specifically, I am interested in performance, ease of operation, wet
imaging, maintenance issues and support.


Thanks in anticipation.


Samantha Taylor
TDG Experimental Officer
Alcoa World Alumina
XRD, Microscopy and Thermal Analysis
* Phone:(08) 9410 3588
* Fax: (08) 9410 3167
* Email: Samantha.Taylor-at-Alcoa.com.au




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 23:22:35 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 12 Apr 2005 21:40:50 -0700
Subject: [Microscopy] Re: Indexed Color RE: HR images- Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

when the SEM makers work up graphics interfaces, I suspect
that they make simple color presentations. This is the
basis of indexed color. Instead of true color (RBG), they
use a palette of colors which are indexed by the pixel value.
This is typically 4 bits (8 colors) or 8 bits (16 colors).
This reduces the overhead for defining and dealing with
colors. Since the SEM image itself is greyscale, the annotation
and legends are typically of some set of colors. These are
colors out of the palette.

When the image is saved, the result is all grey scale, usually.
hence, the better observable quality. But for those programs
that do not store pure grey scale, Photoshop will allow Mode
change to greyscale. Then, other programs can process the
image file no problem.

JPEG is by definition a true color system--RGB. However, some
apps don't adhere to this. The basic theme is to figure out
what mode your image files are and convert them to greyscale.
Or, convert them to RGB if you want to pseudo color them.

gary g.



At 02:05 PM 4/12/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 03:45:04 2005



From: yezer-at-cc.hut.fi
Date: Wed, 13 Apr 2005 12:03:01 +0300 (EEST)
Subject: [Microscopy] HR images with Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

Thank you for the suggestions and comments for achieving high resolution
images. We will review them and reply with feed back. We are looking for a
shareware program for calculating thin films thickness by SEM. If any one know
one and can recommend we will appreciate.

Regards,
Ezer Yossef


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 08:09:31 2005



From: cpotter-at-scripps.edu (by way of MicroscopyListserver)
Date: Wed, 13 Apr 2005 08:28:39 -0500
Subject: [Microscopy] viaWWW: A Practical Course in Molecular Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cpotter-at-scripps.edu) from http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April 12, 2005 at 14:26:52
---------------------------------------------------------------------------

Email: cpotter-at-scripps.edu
Name: Clint Potter

Title-Subject: [Microscopy] [Filtered] A Practical Course in Molecular Microscopy

Question: The National Resource for Automated Molecular Microscopy
Sponsored by the National Center for Research Resources

Announce

A Practical Course in Molecular Microscopy
November 2-10, 2005
National Resource for Automated Molecular Microscopy
The Scripps Research Institute (TSRI), La Jolla, California.

We invite applications to participate in a Practical Course in Molecular Microscopy. This biennial course is part of the outreach and training activities of the NCRR National Resource for Automated Molecular Microscopy (NRAMM), at the Center for Integrative Molecular Biosciences (CIMBio) at The Scripps Research Institute (TSRI). The course is aimed at approximately 40 participants who want a thorough grounding in all aspects of molecular structural determination by cryo-electron microscopy (cryoEM).

The course will include an extensive exposure to all of the practical aspects of cryoEM as well as a solid grounding in the theory. The basic format will be theoretical lectures in the morning followed by hands-on lab sessions and demonstrations in the afternoon. The lab sessions will include instruction in specimen preparation, use of the electron microscope for imaging of cryo specimens, evaluation of the images, and a thorough treatment of the image analysis techniques required to reconstruct three dimensional maps. In addition there will be a number of afternoon lectures introducing participants to research topics in the area of macromolecular structure. Evenings will be reserved for presentations by participants and for helping participants with their own projects.

The instructors for the course include: Francisco Asturias (TSRI), Tim Baker (UCSD), Charlie Brooks (TSRI), Nicolas Boisset (Paris), Anchi Cheng (TSRI), Wah Chiu (Baylor), David de Rosier (Brandeis), Joachim Frank (Albany), Yoshi Fujiyoshi (Kyoto), Bob Glaeser (Berkeley), Niko Grigorieff (Brandeis), Dorit Hanein (Burnham), Elizabeth Kubalek (TSRI), Steven Ludtke (Baylor), Ron Milligan (TSRI), Pawel Penczek (Houston), Clint Potter (TSRI), Tanvir Shaikh (Albany), Vinzenz Unger (Yale), Nigel Unwin (MRC), Neils Volkmann (Burnham), Thomas Walz (Harvard) and Mark Yeager (TSRI).

Applications forms may be found online at the workshop web page:
http://nramm.scripps.edu/seminars/2005/cryoem/
Applicants are asked to provide a CV, publication list, and a short description of their current work and future plans. In addition participants will be asked to state a preference for the particular area of image analysis (single particles, viruses, helices, 2D crystals) on which they wish to focus. This will help balance the participation in the lab sessions.

A registration fee of $450 will be charged to participants from non-profit institutions. Meals will be provided as part of the course registration fee but participants will be expected to cover their own travel and lodging expenses. Cost of housing is approximately $60/night for a shared room. The course organizers will help coordinate room sharing arrangements.

The application deadline is 1 July and a final selection of participants will be made by 1 August.

Please see all other information pertaining to the course at the web site:
http://nramm.scripps.edu/seminars/2005/cryoem/

For other inquiries send email to: amiadmin-at-scripps.edu

Organizers
Bridget Carragher, Clinton S. Potter and Ronald A Milligan
National Resource for Automated Molecular Microscopy,
The Scripps Research Institute


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 08:08:57 2005



From: somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Wed, 13 Apr 2005 08:28:06 -0500
Subject: [Microscopy] viaWWW: thanks Dr Warren E Straszheim for coments about pollen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://www.microscopy.com/MLFormMail.html on Tuesday, April 12, 2005 at 13:52:20
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh

Organization: urmia university

Title-Subject: [Microscopy] [Filtered] thanks Dr Warren E Straszheim for coments about pollen grian counting

Question: Hello Dear Dr Warren

I appreciate your helpful reply about the reason of reuction
in pollen grain counting.
you are right, I have mentioned the big size of pollen grains being kept in water after oneday.
sincerely
Somayyeh

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 08:08:27 2005



From: yagerra-at-aol.com (by way of MicroscopyListserver)
Date: Wed, 13 Apr 2005 08:27:35 -0500
Subject: [Microscopy] viaWWW: good starting microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (yagerra-at-aol.com) from http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April 12, 2005 at 12:52:49
---------------------------------------------------------------------------

Email: yagerra-at-aol.com
Name: Robert A. Yager

Organization: Yager Laboratories

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I want to expand my consulting to optical microscopy analysis of defects in organic coatings. Can anyone recommend a good starting microscope (low $$).



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 09:27:29 2005



From: Ciaburri, Diane A :      Diane.Ciaburri-at-gd-ais.com
Date: Wed, 13 Apr 2005 10:43:40 -0400
Subject: [Microscopy] Job Opening - Failure Analyst

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

We have an opening for a failure analyst. Please direct all inquiries
to me.

Diane Ciaburri
General Dynamics Advanced Information Services
Pittsfield MA
(413) 494-3430




Posted Title: Senior Engineer - Electrical (Failure Analysis)

Job Location: Massachusetts - Pittsfield

Job Requirements:

The candidate should have a minimum of 3-5 years experience in the
evaluation and failure analysis of materials relating to both Electrical
and Mechanical components. Knowledge of Materials Science and Analysis
Lab experience is required. Experience in one or more of the following
is preferred: Scanning Electron Microscopy, Infrared & Optical Imaging,
metallurgy, thermal analysis, chemistry, coating techniques, X-ray
analysis. The candidate must be proficient with Microsoft Windows and
be capable of utilizing software tools relative to image processing
(Image Pro, Adobe, etc.) The candidate must also be proficient at
developing and presenting both oral and written test reports and be an
effective communicator who can work "real-time" with designers to solve
complex issues. The Senior Engineer - Electrical will be an active
participant in implementing and improving the GDAIS Common Process
relative to both failure analysis and general processes.

BS (EE, ChemE, Physics, Chemistry, Materials Engineering) or related
area with a minimum of 3 to 5 years experience in material analysis or
related areas (Required)
MS (Desired)

Other Job Requirements:
Strong communication and team skills.
Dedication to ethics, diversity and safety.
Experience in the following: Scanning Electron Microscopy, Infrared &
Optical Imaging, metallurgy, thermal analysis, chemistry, coating
techniques, X-ray analysis. (Preferred)
Experience with Microsoft Windows (Required)
Knowledge of Image processing tools and techniques (Preferred)
Proficient at identifying and developing process and quality
improvements.
Dedication to meeting assigned budgets and schedules.
Minimum travel required.
Relocation negotiable


Specific Responsibilities:

The Senior Engineer - Electrical (Failure Analysis) is responsible for
designing and conducting tests for physical, electrical, and mechanical
attributes to determine production/process anomalies, causes of failure,
and recommend corrective action.

Responsibilities include:

Perform material evaluation and failure analysis of samples provided by
Engineering and Operations.
Work with designers to understand underlying issues leading to a root
cause of the failure.
Generate reports and oral presentations documenting the tests performed,
data taken and conclusions/recommendations.
Improve current material evaluation and failure analysis processes and
techniques.




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 09:30:04 2005



From: Scott Griffith :      skod-at-ises-llc.com
Date: Wed, 13 Apr 2005 08:49:03 -0600
Subject: [Microscopy] Re: Searching for Leitz Ergolux AMC documentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Well, here's a report of customer service that exceeded my expectations by
a good fraction. Within 12 hours of my plea for Ergolux documentation, I
got a response from not only Ms. Ziegler below, but also from the Leica
Microsystems Denver area dealer (Gary Hanson), who is just a few miles
away. So, it would appear that I have simply been talking to the wrong
people- showing once again the value of widely-distributed lists like this.

I now have some documentation on the way from Mr. Hanson, which should
help somewhat with restoring the stand. I still need documentation on the
stage and electronics rack (particularly cable pinouts), which will
hopefully come from Germany or from some helpful list member- so don't
stop dusting off any old manuals, please! But the instrument is now a lot
closer to being productive again than it was.

My thanks to Ms. Ziegler to making the extra effort. Unsupported hardware
or not, there are still folks out there who want to take care of their
customers.

On Tue, 12 Apr 2005 07:50:10 -0500,
{Gretchen.Ziegler-at-leica-microsystems.com} wrote:

} I will send out a search with our dealers, and see if anyone in Germany
} has anything for you.
}
} Gretchen
}
} Gretchen Ziegler, Sales Manager, Educational Division
} Leica Microsystems, Inc.
} P.O. Box 151 - Ocean Grove, NJ 07756
} Phone: 732-897-9506 - Fax: 847-236-3013
} Voicemail: 1-800-248-0665 ext. 5131

-skod


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 09:34:38 2005



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Wed, 13 Apr 2005 15:53:31 +0100 (BST)
Subject: [Microscopy] Relocating a slide position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

The old chestnut about relocating that area of
interest on a slide. I've used my own England Finder,
but have not recently used any other methods on a
non-motorised stage.

Anyone out there with alternative favourite methods?

I want to ensure that I include other possibilities
within a lecture I'm going to give....

Regards,
Jeremy

Send instant messages to your online friends http://uk.messenger.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 14 09:12:22 2005



From: gazzoman-at-hotmail.com (by way of MicroscopyListserver)
Date: Thu, 14 Apr 2005 07:33:50 -0500
Subject: [Microscopy] viaWWW: PET-100x

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gazzoman-at-hotmail.com) from http://www.msa.microscopy.com/MLFormMail.html on Wednesday, April 13, 2005 at 14:44:27
---------------------------------------------------------------------------

Email: gazzoman-at-hotmail.com
Name: Daniele Gazzola

Organization: university of bologna

Title-Subject: [Microscopy] [Filtered] PET-100x

Question: I would appreciate if somebody could let me know if exists an immersion oil with which I can use a 100x obj. with a PET coverslip.
I believe that PET index of refraction is about 1.64.

Thanks,
Daniele

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 14 10:05:31 2005



From: ke :      kevans1-at-nycap.rr.com (by way of Ask-A-Microscopist)
Date: Thu, 14 Apr 2005 07:30:15 -0500
Subject: [Microscopy] AskAMicroscopist: Microscope restoration Project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello

I wonder if you might be able to help me.

My name is Kenneth Evans and I am a retired Chemistry instructor in upstste New York (Albany region). I have recently acquired a microscope which I am intending to restore. I have been unable to find but four or five references to it on the internet and therefore I have been asking for help from people like yourself .

The scope may be called a Quantimet 720 and it may have been part of a metallurgical system produced by a company called IMANCO in or about 1969. I do not know if they produced the scope or just the "system" in which the scope was used. There seems to be little record of them on the net.

Any information you might be able to provide would be greatly appreciated.


Thank you1

kenneth



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 14 11:51:20 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Thu, 14 Apr 2005 10:10:29 -0700 (PDT)
Subject: [Microscopy] Re: AskAMicroscopist: Microscope restoration Project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Kenneth:

I believe the microscope was part of a much larger
unit, similar to the relic I once tried to resurrect
at my previous position. The Quantimet unit was used
for image analyzing. I imagine the microscope can be
used on its own without the image analyzing system.

Are there any other brand names on the microscope? I
find it hard to imagine they made a microscope
dedicated just for this system, versus adapting an
existing make to their unit.

Stu Smalinskas, PE
Metallurgist
SKF
Plymouth, Michigan

--- ke {kevans1-at-nycap.rr.com} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
}
} Hello
}
} I wonder if you might be able to help me.
}
} My name is Kenneth Evans and I am a retired
} Chemistry instructor in upstste New York (Albany
} region). I have recently acquired a microscope
} which I am intending to restore. I have been unable
} to find but four or five references to it on the
} internet and therefore I have been asking for help
} from people like yourself .
}
} The scope may be called a Quantimet 720 and it may
} have been part of a metallurgical system produced by
} a company called IMANCO in or about 1969. I do not
} know if they produced the scope or just the "system"
} in which the scope was used. There seems to be
} little record of them on the net.
}
} Any information you might be able to provide would
} be greatly appreciated.
}
}
} Thank you,
}
} kenneth
}
}
}



__________________________________
Do you Yahoo!?
Make Yahoo! your home page
http://www.yahoo.com/r/hs


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 14 13:37:32 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 14 Apr 2005 13:29:32 -0400
Subject: [Microscopy] Re: viaWWW: PET-100x

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Cargille labs has a wide range of immersion fluids.
See http://www.cargille.com/opticalintro.shtml



} Email: gazzoman-at-hotmail.com
} Name: Daniele Gazzola
} Organization: university of bologna
} Title-Subject: [Microscopy] [Filtered] PET-100x
}
} Question: I would appreciate if somebody could let me know if exists an
} immersion oil with which I can use a 100x obj. with a PET coverslip.
} I believe that PET index of refraction is about 1.64.
}
} Thanks,
} Daniele

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 07:31:05 2005



From: t.keller-at-uni-jena.de (by way of MicroscopyListserver)
Date: Fri, 15 Apr 2005 07:50:20 -0500
Subject: [Microscopy] viaWWW: Jeol operation at different voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (t.keller-at-uni-jena.de) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 15, 2005 at 03:54:57
---------------------------------------------------------------------------

Email: t.keller-at-uni-jena.de
Name: Thomas Keller

Organization: University of Jena

Title-Subject: [Microscopy] [Filtered] Jeol operation at different voltage

Question: Dear all,

I would like to operate our TEM (JEOL 3010) at different voltages (300 kV, and 120 kV / 200 kV). Is that possible without much realigment each time?

Thanks for your help
Thomas Keller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 07:31:18 2005



From: helen.gruber-at-carolinashealthcare.org (by way of MicroscopyListserver)
Date: Fri, 15 Apr 2005 07:50:43 -0500
Subject: [Microscopy] viaWWW: TEM tech position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (helen.gruber-at-carolinashealthcare.org) from http://www.msa.microscopy.com/MLFormMail.html on Friday, April 15, 2005 at 06:11:02
---------------------------------------------------------------------------

Email: helen.gruber-at-carolinashealthcare.org
Name: Helen E. Gruber, Ph.D.

Organization: Carolinas Medicial Center, Charlotte, NC

Title-Subject: [Microscopy] [Filtered] MListserver: TEM tech position available

Question: Transmission Electron Microscopy technician position is available immediately. Clinical/research work. Certification preferred but not essential. Previous TEM experience required; immuno experience is advantageous. "Hard money" hospital funded position (not grant funded). Salary range is for a Research Lab Tech II, $13.10-19.65/hour. 401k and health/dental benefits. Send me your resume, history of your TEM experience, and names and contact info for 3 references.

Carolinas Medical Center is an Academic Medical Center Teaching Hospital and the flagship facility of Carolinas HealthCare System. Carolinas HealthCare is an equal-opportunity, not-for-profit, self-supporting public organization offering a wide variety of health and human services to residents of both North and South Carolina. Carolinas HealthCare System is the largest healthcare system in the Carolinas, and fourth largest public system in the nation

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 07:32:34 2005



From: tony.j.savage-at-gsk.com (by way of MicroscopyListserver)
Date: Fri, 15 Apr 2005 07:51:59 -0500
Subject: [Microscopy] AskAMicroscopist: Microscope restoration Project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html





The Quantimet 720 was an early image analyser which was developed and manufactured in the UK by a Company called Cambridge Instruments. A few years ago, this company was amalgamated into the Leica Group. I suspect that there may be a few people around who could still help you in this restoration project. You could try by approaching their UK address: Leica UK Ltd, Davy Avenue, Knowl Hill, Milton Keynes. MK5 8LB. UK. (tel. 00 44 01908 666663). Good luck
Regards,
Tony
Histopathology Group
Asthma Biology Department.
RIRP CEDD.
GlaxoSmithKline Medicines Research Centre,
Gunnelswood Road,
STEVENAGE,
Hertfordshire.
SG1 2NY
tel. +44 (0)1438 764117
fax. +44 (0)1438 764782
email. Tony.J.Savage-at-gsk.com
mobile +44 07753609835
http://ukdiscovery.gsk.com/histopathology/default.htm


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 07:58:31 2005



From: frank.karl-at-degussa.com
Date: Fri, 15 Apr 2005 08:23:03 -0400
Subject: [Microscopy] Re: Re: viaWWW: PET-100x

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html





The bigger question is, is the working distance of the oil emersion lens
long enough to ue a PET cover slip. In many cases a number 1 slip is used
instead of a 1.5 slip. Some folks have been known to put the specimen on
the slip (blood smear or thin section) and not the slide. Working distance
rules in oil emersion. Don't forget to use an oilable condenser, 'cause
resolution is a function of the lowest refractive index in the
condenser/sample/objective system.

Best wishes and good luck!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



Michael Cammer
{cammer-at-aecom.yu. To: gazzoman-at-hotmail.com (by way of MicroscopyListserver),
edu} microscopy-at-microscopy.com
cc:
04/14/2005 01:29 Subject: [Microscopy] Re: viaWWW: PET-100x
PM







------------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Cargille labs has a wide range of immersion fluids.
See http://www.cargille.com/opticalintro.shtml



} Email: gazzoman-at-hotmail.com
} Name: Daniele Gazzola
} Organization: university of bologna
} Title-Subject: [Microscopy] [Filtered] PET-100x
}
} Question: I would appreciate if somebody could let me know if exists an
} immersion oil with which I can use a 100x obj. with a PET coverslip.
} I believe that PET index of refraction is about 1.64.
}
} Thanks,
} Daniele

____________________________________________________________________________

Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of
Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is
privileged.**







From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 09:32:02 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Fri, 15 Apr 2005 12:11:32 -0230
Subject: [Microscopy] Rolling shutter digital microscope cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

MSA :o)

Can someone explain the how "rolling shutter" digital cameras are different?
I'd especially like to know how this difference affects practical use for
applications in microscopy.

Tia & cheerios ... michael shaffer :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 09:59:55 2005



From: Mike McKay :      mike.mckay-at-pixelink.com
Date: Fri, 15 Apr 2005 11:19:06 -0400
Subject: [Microscopy] Rolling shutter digital microscope cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Michael,

An electronic rolling shutter is similar to a drop-curtain shutter on a film
camera. With a Rolling Shutter, only a few rows of pixels are exposed at
one time. The camera builds a frame by reading out the most exposed row of
pixels (and ceasing exposure of that row), starting exposure of the next
unexposed row down in the Region of Interest (ROI), then repeating the
process on the next most exposed row and continuing until the frame is
complete. After the bottom row of the ROI starts its exposure, the process
"rolls" to the top row of the ROI to begin exposure of the next frame's
pixels.

The exposure down each frame, and from frame-to-frame, remains consistent
due to this continuous read-out.

The row read-out rate is constant, so the longer the exposure setting, the
greater the number of rows being exposed, or integrated, at a given time.
Rows are added to the exposed area one at a time. The more time that a row
spends being integrated, the greater the electrical charge built up in the
row's pixels and the brighter the output pixels will be. As each fully
exposed row is read out, another row is added to the set of rows being
integrated.

Rolling Shutter provides evenly exposed image data with the greatest
possible speed. Because of its speed, a camera in rolling shutter mode is
programmed to free-run, that is to sends frames across the bus as fast as it
can.

Each row of pixels has a slightly different exposure start and end times
from the adjacent rows, so Rolling Shutter can produce a distorted effect
when imaging fast moving subjects, even with very short exposure times. The
distortion is due to the comparatively lengthy process of readout compared
to exposure.

As an example, if using a PixeLINK Pl-A686 camera with 6.6 megapixel sensor,
to readout the entire frame requires approximately 250 milliseconds. While
a short exposure may stop a moving object, the same object can move
appreciably in the quarter second that it takes to readout the frame
resulting in distortion in the direction of motion. The distortion will be
less noticeable on sensors with faster readout times, smaller resolutions
(fewer rows in the ROI) or if strobe/flash illumination is used.

For best results

Rolling shutter should be used with constant illumination and with a static
subject.



Yours,


Michael McKay | Product Manager | PixeLINK
3030 Conroy Road Ottawa, ON K1G 6C2
tel: 613.247.1211 x 152 | fax: 613.247.2001 | http://www.pixelink.com/















-----Original Message-----
} From: michael shaffer [mailto:michael-at-Shaffer.net]
Sent: April 15, 2005 10:42 AM
To: MSA Listserver

MSA :o)

Can someone explain the how "rolling shutter" digital cameras are different?
I'd especially like to know how this difference affects practical use for
applications in microscopy.

Tia & cheerios ... michael shaffer :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 10:49:59 2005



From: MelissaRanieri21-at-aol.com
Date: Fri, 15 Apr 2005 12:09:13 EDT
Subject: [Microscopy] IXRF Open House @ Hitachi Maryland

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

IXRF Systems, Inc. Open House

IXRF would like to invite anyone to an open house being held at Hitachi's
facility in Gaithersburg, MD May 16-18, 2005. IXRF will be demonstrating on a
Hitachi S4800 FE the EDS2004 as well at the new fX SEM Tube that incorperates
XRF analysis in the SEM. We will be scheduling demonstrations on these 3
days from 9 am - 4 pm. For more information on these products please visit our
website at www.ixrfsystems.com or find us on www.microscopy.info.

Please contact Melissa Ranieri -at- melissa-at-ixrfsystems.com or call #
281-286-6485 for further details.

Thank you and have a great weekend everyone!



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 11:10:23 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Fri, 15 Apr 2005 11:29:39 -0500
Subject: [Microscopy] RE: re new anything

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I thought that after the initial emotional impact of the "new anything" post
had worn off a bit, in favor of an alternate opinion, I thought that I'd
contribute my own $0.02 worth here.

So I thought that I'd give my own point by point thoughts on this post,
since it seems to have been provoked by my own post on new ultramicrotomes,
which was primarily prompted by my need for 3 different quotes before a
major purchase can be made in most cases, and in a lot of institutions.

There is indeed brand loyalty that can happen, and sometimes with good
reason, though it is true that new developments may occur, pricing policies
can change, etc. That said, and given that the United States and Canada are
supposedly free countries, I don't see anything particularly wrong or
objectionable about expressing praise or one's brand loyalties. It's sort
of like the Toyota vs. Honda thing. Everyone ultimately realizes that we
are just dealing with opinions, and people also realise that there is a
brand loyalty thing in many cases. And if you are test driving a Toyota
Corolla, the sales person should be aware that you've probably test driven a
Honda Civic. [both of them are good cars by the way!]

That said, I wouldn't myself say too many things about sales, service staff,
since I not only like to give people the benefit of the doubt, but also,
because overall my experience with people in commercial sales has been very
good. So I would agree that it is not desirable to debate someone's
qualifications and style in public. I guess I missed the part of the thread
here that did that.

But when it comes to the point about keeping specific product names off the
listserver, this is a totally different issue. To praise one product
profusely is not necessarily to trash another product. In the post that I
made about scanners for example, I found it extremely helpful to get
specific product names, and model numbers. When I have many people telling
me the same thing, it gives me a lot of confidence in a particular product,
so that I am better able to read between the lines when I read up on the
specs of a given product. It doesn't mean that other products are bad, just
that for a given task, one product might be better suited. And one also has
to remember that price is another variable that keeps everyone from buying
the Mercedes of the lot. I find that generic comments about a product are
almost useless in my opinion, because the main questions that you have are
still left unanswered.

I have participated in many forums on many different sort of topics, and my
experience is that most people don't find a mention of specific products
objectionable, as long as people respect the opinions of those with a
dissenting opinion. Indeed, this can be a way to help other products,
because if they really do have merit, someone who owns it will notice it,
and communicate that to others, especially if such a product is quite a bit
less expensive that the frontrunner.

You can have education as a prime purpose of a listserver where there is
some brand endorsement, and the education won't suffer. People are
generally able to differentiate between the 2 without any adverse affects.

To give a concrete example in another area, I might mention the Nikon vs
Canon debate that one might see on photography forums. Most people can
recognize that it's ultimately a matter of preference, and no doubt those
who own Nikons secretly want into the Canon camp, and vice-versa, but if one
is considering buy a given camera, it is very valuable to be able to hear
comments from an owner of such a camera directly, without having to encode
such talk in generics. And usually the better photographers realize that
the main reason that they have to stick to one manufacturer is primarily
because they have so much invested in lenses, that it's just too expensive
to change sides. One likes to hear about the pros and cons of not only a
given manufacturer, but also a given model and the accessories that are
available for that model. Because if the accessories are not available for
a certain model, that one needs for a specific purpose, then it's better to
know that beforehand than after the purchase. Each manufacturer and model
has it's own strenghts and weaknesses, and it's helpful to know exactly what
these are when one is making a decision about an expensive product. It's
less important when one is buying a pencil.

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 11:24:32 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Fri, 15 Apr 2005 11:43:50 -0500
Subject: [Microscopy] Re: Re: Re: viaWWW: PET-100x

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

One point to remember: the coverslip is an optical element, just like the lenses and prisms in the microscope. The reason that we use #1-1/2 coverslips is the optical path, which is the thickness (0.17mm) times the refractive index (typically about 1.52).
OP = n x t

If the optical path is too thick, you will get spherical aberration. The two best symptoms are that you can't really find a plane of focus and that the image looks milky or "soft".

Since PET has a has RI (n) of 1.64, it makes perfect sense to move down to a #1 or even a #0. My recommendation - get some and try them with YOUR sample.

Hope this is helpful.

Best regards,
Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). For details, call us at 972-943-8011 and ask for Ken Piel.

At 07:23 AM 4/15/2005, frank.karl-at-degussa.com wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 13:03:04 2005



From: Tracy Challman :      tchallman-at-forensic-engrs.com
Date: Fri, 15 Apr 2005 12:38:53 -0700
Subject: [Microscopy] S-2460N Independent Service Contractors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Sorry for any delay but I have been working overseas and could not download
my mail.

Changing the working distance in any SEM varies the type and levels of
signal reaching the Everhart-Thornley detector. Finding a position which
maximises BSE reduces the appearance of charge. At the higher voltage BSE
are not effected by the charge to the same extent as the lower energy SE. An
example of a typical position for the maximum BSE contribution, therefore
the minimum charge position, on Japanese instruments with a conventional
detector position in the specimen chamber is 15mm WD and 45degs of tilt .

The simple way of determining the "minimum charge" position is to form an
image and monitor its wave form. Without changing the gain move the sample
to another position and view the wave form at the same magnification. You
will see a rise or fall depending upon the signal level change. The point
of highest signal will be that of the highest levels of backscatter or
converted backscatter entering the detector; the secondary levels remain
relatively constant.

For more information and ideas on signals take a look at our web site in
Hints and Tips

Regards

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel +44 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com

----- Original Message -----
} From: Mark Robertson-Tessi {mtessi20-at-yahoo.com}
To: {Microscopy-at-microscopy.com}
Sent: Tuesday, April 12, 2005 5:11 PM

Dear MSA Listserver Colleagues,
 
I have been asked to find alternative service contractors / independent
contractors for our Hitachi S-2460N SEM.
 
I am aware that I may be the one selected as the alternative contractor,
however, I would be very interested to know if others have reliable people
working on their instruments aside from Hitachi's own field engineers.  I
have been VERY pleased with their services.  This issue is cost related,
with hopes of maintaining the quality.
 
Sincerely,
Tracy
 
Tracy Challman
Materials Scientist/Ceramic Engineer
Schaefer Engineering Corporation
23109  55th Ave West
Mountlake Terrace WA 98043-4711
 
Tel.:  425-775-5550    Toll-free:  800-711-0704    Fax: 425-775-0900
 
tchallman-at-forensic-engrs.com       www.forensic-engrs.com
 



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 22:44:52 2005



From: amr2w-at-virginia.edu (by way of MicroscopyListserver)
Date: Fri, 15 Apr 2005 23:04:12 -0500
Subject: [Microscopy] viaWWW: 3-D Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://www.msa.microscopy.com/MLFormMail.html on Friday, April 15, 2005 at 15:22:42
---------------------------------------------------------------------------

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: University of Virginia

Title-Subject: [Microscopy] [Filtered] 3-D Reconstruction

Question: Hello All,
I am interested in doing 3-D reconstruction using the SEM, but not by sectioning. There are a few programs out there that will generate a 3-D model from simply a height map and texture image or from just a stereo pair. Is there a program out there that will use micrographs from multiple angles/rotations for a more true 3-D volume? Thanks for the help!


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 16 00:07:18 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 15 Apr 2005 22:26:40 -0700
Subject: [Microscopy] viaWWW: 3-D Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Try:
http://www.alicona.com/

John Mardinly

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:amr2w-at-virginia.edu]
Sent: Friday, April 15, 2005 9:04 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (amr2w-at-virginia.edu) from
http://www.msa.microscopy.com/MLFormMail.html on Friday, April 15, 2005
at 15:22:42
------------------------------------------------------------------------
---

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: University of Virginia

Title-Subject: [Microscopy] [Filtered] 3-D Reconstruction

Question: Hello All,
I am interested in doing 3-D reconstruction using the SEM, but not by
sectioning. There are a few programs out there that will generate a 3-D
model from simply a height map and texture image or from just a stereo
pair. Is there a program out there that will use micrographs from
multiple angles/rotations for a more true 3-D volume? Thanks for the
help!


------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Sat Apr 16 08:19:15 2005



From: Bill Miller :      microbill-at-mohawk.net
Date: Sat, 16 Apr 2005 09:38:31 -0400
Subject: [Microscopy] Re: viaWWW: 3-D Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Alicona's MeX software will do this..

Bill Miller

At 12:04 AM 4/16/2005, amr2w-at-virginia.edu wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Sun Apr 17 19:49:36 2005



From: James Pawley :      jbpawley-at-wisc.edu
Date: Sun, 17 Apr 2005 20:12:00 -0500
Subject: [Microscopy] Re: RE: HR images-Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} }
} } unique and experiment.
}
} Please forget about SE from greater depths and concentrate on balancing the
} information you require. Very short WD ( {5) on a 4500/4700 restricts the
} imaging data to SE which can be very boring and this restriction vastly
} increases the possibility of charge. Move away from the lens a little (~7
} to 9mm) and you bring in some of the BSE information that has been converted
} to secondaries, these will provide added contrast, some image depth and most
} of all less charge.

Hi all,

I think that there is a way that both are right.

True, SE do not originate from more than about 50 nm inside most specimens.

However, the BSE that do can produce SE "on their way out", as it
where. In fact, on metals, above about 5kV, most SE are produced in
just this way.

With a good modern FEG SEM, I, myself, prefer 1- 1.5 kv for lightly
coated biological specimens.

As far at the "less charge" claim, a lot of SE are produced from BSE
hitting the polepiece, and, I suppose depending on its shape this may
be more or less with longer WD. Although I guess that these might
affect the actual surface charge level (by scattering back to the
sepcimen?), it is important to note that seeing the "anomalous
brightness" effects of charging is merely an indication that the
charge present has messed up the SE collection fields, (usually to
decrease the collection efficiency from nearby details but sometimes
to increase it from the "charged" feature) and I think that we can
assume that the SE collection field in a "below-lens" SEM will
increase (and so be harder to disrupt ) at longer WD.

Cheers,

Jim P.
--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 11-23, 2005, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2005


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 10:17:43 2005



From: Iain Miller :      milleri-at-ohio.edu
Date: Mon, 18 Apr 2005 11:30:03 -0400
Subject: [Microscopy] SEM 3-D reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Photomodeler can be adapted for use with scanning micrographs
http://www.photomodeler.com/

The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (amr2w-at-virginia.edu) from
http://www.msa.microscopy.com/MLFormMail.html on Friday, April 15, 2005 at
15:22:42
---------------------------------------------------------------------------

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: University of Virginia

Title-Subject: [Microscopy] [Filtered] 3-D Reconstruction

Question: Hello All,
I am interested in doing 3-D reconstruction using the SEM, but not by
sectioning. There are a few programs out there that will generate a 3-D
model from simply a height map and texture image or from just a stereo pair.
Is there a program out there that will use micrographs from multiple
angles/rotations for a more true 3-D volume? Thanks for the help!


---------------------------------------------------------------------------

Iain Miller
Department of Biological Sciences
Ohio University
Athens, OH 45701

740-593-2120
milleri-at-ohio.edu


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 10:18:49 2005



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Mon, 18 Apr 2005 10:37:05 -0500
Subject: [Microscopy] TEM-200CX SHP polepiece

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi All:
I am looking for someone who has a 200 CX TEM and uses it (or has used
it) with the SHP pole piece or is knowledgeable on the different
configurations possible with the different pole pieces. There are
several pole piece/objective aperture configurations for the 200CX but
we have very little JEOL documentation on the subject. Any real world
experience, expertise or literature that could be shared would be
greatly appreciated. Feel free to contact me offline if you think it
makes sense.
Thanks in advance,
Michael Coviello




From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 18:42:02 2005



From: Ian Hallett :      IHallett-at-hortresearch.co.nz
Date: Tue, 19 Apr 2005 12:06:39 +1200
Subject: [Microscopy] Printing grayscale images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking at putting a printer next to our SEM so users can quickly print out a copy of an image without having to go to one of our networked printers elsewhere in the building. I'm interested in good grayscale images - colour is not important. Has anyone any suggestion of printers, I should look at without breaking the budget.

Thanks

Ian

Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 19:49:32 2005



From: kushalseth-at-gmail.com (by way of Ask-A-Microscopist)
Date: Mon, 18 Apr 2005 20:11:55 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: HUVEC CELLS ON COLLAGEN AND GELATIN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kushalseth-at-gmail.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, April 18, 2005 at 19:06:57
---------------------------------------------------------------------------

Email: kushalseth-at-gmail.com
Name: kushal Seth

Organization: Texas tech university

Education: Graduate College

Location: Lubbock, TX, USA

Question: I have to grow HUVEC CELLS ON COLLAGEN AND GELATIN NANO FIBRES . AND VIEW THEM UNDER sem .
Itried to grow andf see them but it had BACTERIAS IT SEEMS RATHER THAN HUVECS.
Please suggest viable steps and procedures or any reference papers.
Thanks

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 19:50:04 2005



From: somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Mon, 18 Apr 2005 20:12:30 -0500
Subject: [Microscopy] viaWWW: preparing herbarium specimens for sectining in paraffin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, April 16, 2005 at 08:53:42
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh

Organization: urmia university

Title-Subject: [Microscopy] [Filtered] preparing herbarium specimens for sectining in paraffin

Question: Hello Dear all

at first thank you very much for helping me doing my experiments
by giving your useful comments.
I want to provide some section from dried herbarium
leaves. Does anybody know how to prepare the dried ones
for Paraffin embedding?
I just know I should boil the leaves for 2-3 minutes.
but can we use FAA for dried leaves and then usual Etoh series for dehydration like FAA preserved materials or
the herbaruim specimens need other treatments?
I appreciate any kind reply.
Sincerely
Somayyeh


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 19:50:24 2005



From: wong2u23-at-yahoo.com (by way of MicroscopyListserver)
Date: Mon, 18 Apr 2005 20:12:53 -0500
Subject: [Microscopy] viaWWW: Vacuum issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (wong2u23-at-yahoo.com) from http://www.msa.microscopy.com/MLFormMail.html on Sunday, April 17, 2005 at 00:14:39
---------------------------------------------------------------------------

Email: wong2u23-at-yahoo.com
Name: Vic Wong

Title-Subject: [Microscopy] [Filtered] MListserver: Vacuum issues

Question: We have a Hitachi s-4500 FESEM which has until recently worked fine. Recently we have had vacuum trouble, unable to get the specimen chamber to pump down. We have changed the oil in the RP and the diff pump, but still remain at the same pressure. The problem occured when I was exchanging a specimen and when I went to pump down, only the low lamp in the S.C. went on. The SEC has the high lamp on. We are down until this problem is fixed, any suggestions?

Vic

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 19:56:25 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 18 Apr 2005 18:18:43 -0700
Subject: [Microscopy] Re: Printing grayscale images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I use HP LJ 2300dn. There is a 2430 USB or LPR. These
are 1200dpi and have adjustable "dot" characteristics.
These are in the 550-600USD range. You have to check for
optional JetDirect if needing TCP/IP interface. If not on
a network, just use the native parallel.

If you want real cheap, look for a used JP LJ 4M+ or 4+
(about $200USD). These are 600dpi. All of the LJs are
workhorses. The later models are much faster and have
toner saving modes. I use the 4+ and 4MP for many years.
The paper output feeder usually fails after 20,000 sheets.
These are still available for about $80...field replaceable.

If you want to spring for color, the new OKI 5430n is
awesome at $1250USD. Ethernet or parallel or USB and
very fast and high quality color. For pure greyscale,
the b/w printers do a better job, IMO.

gary g.


At 05:06 PM 4/18/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 23:02:07 2005



From: Scott Walck on Comcast :      swalck-at-comcast.net
Date: Tue, 19 Apr 2005 00:21:15 -0400
Subject: [Microscopy] Re: Printing grayscale images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gary's solution is similar to what we did at the PPG Glass Technology Center
while I was there. We used a HP LJ2100N which was also 1200 dpi. We set up
our LEO 1350 so that if we printed a 5" wide image on that printer that it
was the same magnification as a stored image. Our standard image output
size was 1024 x 768. However, that did not give us the range in gray levels
of the image. If we were interested in the gray scale of the image, then we
printed an image using the whole page with 1/2" margins. Remember that
there is a tradeoff in grayscale and image pixel resolution for a laser jet
printer because it can only print a dot or not print a dot at the 1200 dpi
resolution. If you are using the 1200 dpi laser printer, the 256 (actually
257) gray levels for a single "pixel" of the image corresponds to an array
of 16 x 16 dots on the printer. At 1200 dpi, this gives 75 16x16 squares
per inch for your image. Your eye has at best (when you were young and
didn't appreciate it) a resolution close to 300 dpi. That means that if a
1024 x 768 digital image is printed at this best eye resolution, it is 3.4"
x 2.56" in size. If I take that same image and print it at 1024 x 768 image
and print it at 10" x 7.5" on a 1200 dpi printer, then I get a factor of
2.9X to expand the gray levels over the image. In other words, my 75 dpi
image now goes to 217 dpi that can be display with all of 256 gray levels.
This assumes a "dumb" laser printer that just uses the 16 x 16 dot array for
printing the grayscale. I don't pretend to know what is going on in the
"smart" laser printers such as the LJ2100N, but the book "Real World
Photoshop" discusses it in much more detail and much more authoritatively
than I can. The net result was that we got pretty good looking images. We
also regularly used the grayscale histogram in the microscope software to
set up our brightness and contrast levels to get as close to using all of
the 256 gray levels as we could.

One other point is that the 2100N was surprisingly fast. I would just wait
until the end of my session and print the images out using Thumbsplus
because I could print the comments and filename along with the image. It
never took very long to print out the images and I would do that as I was
closing down the microscope and removing my sample.

With respect to Gary's suggestion on using the 4+ and 4MP, we had a number
of 600 dpi HP LaserJet printers of this style and some newer styles around
GTC and the quality of the images printed to them depended on the specific
printer. We could get widely varying results printing to the same model
which was not related to the age or usage of the printer. Some simply gave
good results and other didn't. It was a crap shoot. The 1200 dpi printers
always seem to work well for printing images.

I also agree with Gary with respect to color LaserJet printers. The color
laser printers just don't do grayscale images well no matter how you set up
the printer in software.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Monday, April 18, 2005 9:19 PM
To: Ian Hallett
Cc: MSA listserver

I use HP LJ 2300dn. There is a 2430 USB or LPR. These
are 1200dpi and have adjustable "dot" characteristics.
These are in the 550-600USD range. You have to check for
optional JetDirect if needing TCP/IP interface. If not on
a network, just use the native parallel.

If you want real cheap, look for a used JP LJ 4M+ or 4+
(about $200USD). These are 600dpi. All of the LJs are
workhorses. The later models are much faster and have
toner saving modes. I use the 4+ and 4MP for many years.
The paper output feeder usually fails after 20,000 sheets.
These are still available for about $80...field replaceable.

If you want to spring for color, the new OKI 5430n is
awesome at $1250USD. Ethernet or parallel or USB and
very fast and high quality color. For pure greyscale,
the b/w printers do a better job, IMO.

gary g.


At 05:06 PM 4/18/2005, you wrote:


} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 07:38:51 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 19 Apr 2005 09:01:07 -0400
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: HUVEC CELLS ON COLLAGEN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

check out papers by William A Muller, et al
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 07:40:09 2005



From: jacqui.ross-at-auckland.ac.nz (by way of MicroscopyListserver)
Date: Tue, 19 Apr 2005 08:02:30 -0500
Subject: [Microscopy] viaWWW: Nikon EclipseNet Time-lapse Data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jacqui.ross-at-auckland.ac.nz) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 18, 2005 at 21:16:43
---------------------------------------------------------------------------

Email: jacqui.ross-at-auckland.ac.nz
Name: Jacqui Ross

Organization: The University of Auckland

Title-Subject: [Microscopy] [Filtered] Nikon EclipseNet Time-lapse Data

Question: Dear All,

We are using a TE-2000E running through EclipseNet to do time-lapse imaging in 3 channels.

However, when I try to merge the images in order to then create an AVI movie, I can't seem to do it. I can merge individual images but can't do the whole series at once (as a stack).

Can anyone tell me whether it is possible to do this and if so, how?

Your help would be much appreciated.

Cheers,

Jacqui.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 07:40:38 2005



From: diller-at-stefan-diller.com (by way of MicroscopyListserver)
Date: Tue, 19 Apr 2005 08:03:05 -0500
Subject: [Microscopy] viaWWW: manual for the ImageSlave framegrabber-card

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-stefan-diller.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, April 19, 2005 at 02:15:35
---------------------------------------------------------------------------

Email: diller-at-stefan-diller.com
Name: Stefan Diller

Organization: Photography

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear All,
does anyone have a manual for the ImageSlave framegrabber-card available and can email it to me? Or give me hint where to download it?

Thanks a lot,
Stefan Diller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 11:10:40 2005



From: Alexander Solodukhin :      as4j-at-virginia.edu
Date: Tue, 19 Apr 2005 12:33:04 -0400
Subject: [Microscopy] MM2005 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,



Does somebody know where can I find a list of participants or papers
accepted for

the Microscopy and Microanalysis 2005 Conference?



Many thanks in advance.



Sincerely,



Alexander Solodukhin



Department of Anesthesiology

University of Virginia Health System

P.O.Box 800710

Charlottesville VA 22906-0710

Fax: 434-982-0019

Phone: 434-924-2494




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 12:13:41 2005



From: stuart McKernan :      stuartm-at-umn.edu
Date: Tue, 19 Apr 2005 12:36:00 -0500
Subject: [Microscopy] Re: MM2005 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Alexander (and everyone else)

The program is finally in its final state. The list of papers will be
going to Nestor this week and put up on the MSA website. The official
emails from the meeting management will be going out this week also,
now that we have a finalized meeting. We did not want to send out
information with the wrong dates and times for the presentations. It
has taken rather longer than anticipated to get all the sponsoring
societies to agree on the meeting layout.

Having finished we have ended up with the strongest meeting so far with
close to 1100 scientific papers - almost 50% more than previous "good"
meetings!

We look forward to seeing you all in Hawaii.

Stuart McKernan (proceedings editor)

On Apr 19, 2005, at 11:33 AM, Alexander Solodukhin wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear Listers,
}
}
}
} Does somebody know where can I find a list of participants or papers
} accepted for
}
} the Microscopy and Microanalysis 2005 Conference?
}
}
}
} Many thanks in advance.
}
}
}
} Sincerely,
}
}
}
} Alexander Solodukhin
}
}
}
} Department of Anesthesiology
}
} University of Virginia Health System
}
} P.O.Box 800710
}
} Charlottesville VA 22906-0710
}
} Fax: 434-982-0019
}
} Phone: 434-924-2494
}
}
}
}
Prof. Stuart McKernan
IT Characterization Facility, University of Minnesota            
E-mail :  stuartm-at-umn.edu
12 Shepherd Labs,                                                    
                 Office:         (612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455                    
Lab:            (612) 626-7594






From MicroscopyL-request-at-ns.microscopy.com Wed Apr 20 09:41:47 2005



From: Matt Olszta :      mjo10-at-psu.edu
Date: Wed, 20 Apr 2005 11:49:13 -0400
Subject: [Microscopy] JEOL JEE 4C Vacuum Evaporator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Bonjour à tous!

A researcher working in the Chemistry department here at UM has asked
we a question that I wasn't that comfortable answering so I suggested
we could solicit some other opinions on the EM list. My experience with
the list has shown me there are many unselfish folks out there with a
wealth of knowledge. Maybe one day I will have chance to meet a few of
you. Thanks in advance for your comments and suggestions.
Merci,

André

} From: gthorne-at-Ms.UManitoba.CA

All,
We recently resurrected an old JEOL JEE 4C vacuum evaporator for carbon
coating.
We have the instructions and everything works well on the instrument. I was
wondering if anyone who might have used one of these systems in the past could
give some advice on carbon tip geometry and deposition times. It would be
nice
to put down a thick coating of carbon before I start FIB milling.

Matt Olszta, Ph.D.
Materials Research Institute
Penn State University



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 20 13:18:06 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 20 Apr 2005 11:55:33 -070