} We are comparing dye sub printers [...]. It is my understanding } that both produce good quality, gray-scale prints (versus } dithered, inkjet prints). ...
Inkjet printers may dither, but you cannot see the dithering at all with modern printers. For excellent color, excellent grayscale, as well as archival prints, I would suggest you take a look at the 9-ink HP Photosmart 8750. It's only downside is a thirst for ink cartridges, but considering the prices you are comparing, this printer would be a bargain.
Genuinely, Michael Shaffer :o) SEM/MLA Lab Coordinator (709) 737-6790 (Ofc) (709) 737-6790 (Lab) {www.micro-investigations.com} {www.esd.mun.ca/epma/} Inco Innovation Centre Memorial University of Newfoundland St. John's, Newfoundland
From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 07:22:43 2005
We have a Canon i9900. The prints are outstanding and in a way, sadly better than anything I could do in the darkroom.
Alan Stone ASTON
At 07:13 AM 7/1/2005, you wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Alan Stone ASTON Metallurgical Services Co., Inc. 200 Larkin Drive Ste A Wheeling, IL 60090 847/353-8100 www.astonmet.com
From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 08:08:20 2005
For a few years Pictrography was my printer of choice (average cost per print is about $2 or $3). Half a year ago I bought cheap inkjet HP Deskjet 6540 for draft prints. Surprisingly, it produces grayscale prints of very good quality, so now I practically stopped using Pictrography.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 3127 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: John J. Bozzola [mailto:bozzola-at-siu.edu] } Sent: Thursday, June 30, 2005 4:14 PM } To: Microscopy-at-msa.microscopy.com } Subject: [Microscopy] dye sub versus Pictrography } } } } } -------------------------------------------------------------- } ---------------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ----------------- } } We are comparing dye sub printers versus the Fuji Pictrography } PG4500. The price differential is a staggering $22,000 versus $6,500, } respectively. It is my understanding that both produce good quality, } gray-scale prints (versus dithered, inkjet prints). Why the price } differential? I would welcome any comments, user experiences, etc. } Does anyone know the average cost per 8.5 x 11in print? } } Thank you. } -- } ############################################################## } John J. Bozzola, Ph.D., Director } I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) } 750 Communications Drive - MC 4402 } Southern Illinois University } Carbondale, IL 62901 U.S.A. } Phone: 618-453-3730 } Email: bozzola-at-siu.edu } ############################################################## } }
From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 08:37:32 2005
I have to agree with the ink jet crowd. I have a Canon i9100 for personal use which will make prints up to 19x13 inches and it also gives me much more control than I had in the darkroom (and John Bozzola knows what a switch it is to hear that from me---I used to live in darkrooms!). I simply couldn't justify paying thousands of dollars for high-end dye-sub printers or the Fuji system in our lab, when a $500-600 printer will give excellent quality prints with a life span of 100+ years, using the right inks and papers.
I honestly don't even remember when we made the last print for our users in the lab. I bought a cheaper, but good quality ink-jet a couple of years ago for people who wanted prints and the original ink cartridges are still in it. We shoot film on the TEM and scan it and our SEM takes digital images directly. We give our clients the images on disks and they're happy. Occasionally we do quick work prints on a laser printer upon request, but even this is rare.
I still have a darkroom and two enlargers in storage in my garage, but it's becoming more out of nostalgia than any real hope I'll set them up again someday. I just can't let go......
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
-----Original Message----- } From: Alan Stone [mailto:as-at-astonmet.com] Sent: Friday, July 01, 2005 7:22 AM To: microscopy-at-microscopy.com
We have a Canon i9900. The prints are outstanding and in a way, sadly better than anything I could do in the darkroom.
Alan Stone ASTON
At 07:13 AM 7/1/2005, you wrote:
} ----------------------------------------------------------------------- } ------- The Microscopy ListServer -- Sponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
} } both produce good quality, gray-scale prints (versus dithered, } } inkjet prints). ... } } Inkjet printers may dither, but you cannot see the dithering at all } with modern printers. For excellent color, excellent grayscale, as } well as archival prints, I would suggest you take a look at the 9-ink } HP Photosmart 8750. It's only downside is a thirst for ink cartridges,
} but considering the prices you are comparing, this printer would be a bargain. } } Genuinely, Michael Shaffer :o) } SEM/MLA Lab Coordinator } (709) 737-6790 (Ofc) } (709) 737-6790 (Lab) } {www.micro-investigations.com} } {www.esd.mun.ca/epma/} } Inco Innovation Centre } Memorial University of Newfoundland } St. John's, Newfoundland
Alan Stone ASTON Metallurgical Services Co., Inc. 200 Larkin Drive Ste A Wheeling, IL 60090 847/353-8100 www.astonmet.com
From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 09:02:28 2005
There is no reason to spend this kind of money to get excellent B&W prints. Many fine art photographers are making beautiful prints with ink jet printers. Check out Lyson.com or inkjetmall.com. Also, continuous inking systems are available that bypass individual ink cartridges. I will post more specifics later or early next week.
Geoff
John J. Bozzola wrote:
} ------------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } } We are comparing dye sub printers versus the Fuji Pictrography PG4500. } The price differential is a staggering $22,000 versus $6,500, } respectively. It is my understanding that both produce good quality, } gray-scale prints (versus dithered, inkjet prints). Why the price } differential? I would welcome any comments, user experiences, etc. } Does anyone know the average cost per 8.5 x 11in print? } } Thank you.
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 10:53:09 2005
ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK (RTP), NORTH CAROLINA AREA
We have an immediate need for a full-time Electron Microscope Technician (maintenance and operation). 3+ years TEM and SEM experience in biological, polymer, carbon/carbon composite, and semi-conductor electron microscopy required. Send resume and salary requirements to sjeffers-at-dgisrd.com.
From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 11:15:37 2005
This post is tangential to the dye sub vs ink jet discussion. When one needs to compare 2 or more images simultaneously, prints can easily be laid out on a table, but this is not so easy with on-screen display of digital images. One thing that we have done in our lab is to install a second monitor at some workstations and increase the Windows desktop size so that it spans the two monitors. This is satisfactory for AFM images where the basic pixel count is 512x512 but may not be so good with other formats. I am curious to learn what other people do.
When you give your customers digital images only, do you feel there is a risk they could miss an important comparison?
regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 19:35:59 2005
Don't forget to use RIP software for excellent tonal range. I've seen photos done with and without and there is a discernible difference and improvement. Damian
-----Original Message----- } From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu] Sent: Friday, July 01, 2005 9:01 AM To: John J. Bozzola Cc: Microscopy-at-msa.microscopy.com
There is no reason to spend this kind of money to get excellent B&W prints. Many fine art photographers are making beautiful prints with ink jet printers. Check out Lyson.com or inkjetmall.com. Also, continuous inking systems are available that bypass individual ink cartridges. I will post more specifics later or early next week.
Geoff
John J. Bozzola wrote:
} -------------------------------------------------------------------------- ---- } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- ----- } } } We are comparing dye sub printers versus the Fuji Pictrography PG4500. } The price differential is a staggering $22,000 versus $6,500, } respectively. It is my understanding that both produce good quality, } gray-scale prints (versus dithered, inkjet prints). Why the price } differential? I would welcome any comments, user experiences, etc. } Does anyone know the average cost per 8.5 x 11in print? } } Thank you.
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
From MicroscopyL-request-at-ns.microscopy.com Sat Jul 2 02:54:09 2005
Don; I tried to purchase an IBM T-220 9 megapixel display, but was told by IBM that they had killed the product. This was the only display ever marketed that could display a 2K X 2K camera image showing all of the pixels on one screen. We still have two screens on our TEM, but need to either display the images at half resolution, or zoom to display only part of the image.
John Mardinly
-----Original Message----- } From: Don Chernoff at ASM [mailto:donc-at-asmicro.com] Sent: Friday, July 01, 2005 9:05 AM To: Microscopy List
This post is tangential to the dye sub vs ink jet discussion. When one needs to compare 2 or more images simultaneously, prints can easily be laid out on a table, but this is not so easy with on-screen display of digital images. One thing that we have done in our lab is to install a second monitor at some workstations and increase the Windows desktop size so that it spans the two monitors. This is satisfactory for AFM images where the basic pixel count is 512x512 but may not be so good with other formats. I am curious to learn what other people do.
When you give your customers digital images only, do you feel there is a risk they could miss an important comparison?
regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
From MicroscopyL-request-at-ns.microscopy.com Sat Jul 2 03:04:41 2005
John; We have a Fuji Pictrograph 3500, and I have to say that nothing I have ever seen prints with the resolution and vivid saturation of the pictrograph, both for color and B&W. However, since all of our conference rooms got digital projectors, we don't make prints any more. I used it to make some absolutely beautiful prints of my daughter, but that's about all. We have had multiple failures of a $1,000 circuit board, and the Fuji service department is a NIGHTMARE to deal with. Right now, the printer is inoperative. The most recent batch of paper and donor sticks to the drum, causing a fault. I don't know if spending another $400 for new rolls will cure that problem, or if there is something else wrong with the printer, but we're probably going to just push it out the door.
John Mardinly Intel
This is the opinion of the author and not of Intel Corporation.
-----Original Message----- } From: John J. Bozzola [mailto:bozzola-at-siu.edu] Sent: Thursday, June 30, 2005 2:14 PM To: Microscopy-at-msa.microscopy.com
We are comparing dye sub printers versus the Fuji Pictrography PG4500. The price differential is a staggering $22,000 versus $6,500, respectively. It is my understanding that both produce good quality, gray-scale prints (versus dithered, inkjet prints). Why the price differential? I would welcome any comments, user experiences, etc. Does anyone know the average cost per 8.5 x 11in print?
Thank you. -- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu ##############################################################
I have updated the security model and the software for the Listserver this morning , and need to perform a full mailing test to all subscribers to confirm functionality. Basically I believe that I have patched a few software holes that spammers have discovered.
I will be monitoring the system for problems the rest of the day. Hopefully this test will run fine and there will be minimal interruptions.
Should you encounter problems please contact me off-line (zaluzec-at-microscopy.com).
Cheers
Nestor Your Friendly Neighborhood SysOp
Sunday July 3, 2005 11:20 AM CST -----------------------------
Two similar models of this monitor were available: IBM T221 and Viewsonic VP2290B, both using IBM display panel 22" diagonal, 3840 x 2400 pixels, 204 dpi. Both discontinued. IBM had intentions of replacing T221 with new model as early as March 2005. I don't know whether they did yet.
We purchased several such monitors, IBM and Viewsonic, for TEM camera systems. Both models are similar in performance, except Viewsonic is a bit slower in full resolution mode, which makes no difference for static images. It's hard for a PC to run 9.2 MP frames at over 20 FPS refresh rate anyway. In fact, it is better to run 1600 x 1200 desktop most of the time and switch to 3840 x 2400 for high resolution viewing. 1600 x 1200 keeps fonts and icons size comfortable, and refresh rate is fast. The display in 3840 x 2400 mode is stunning- like looking through a window on a sunny day. You can take an eye loupe to the screen and see further detail in the image.
If you will be able to find refurbished or second hand T221 - IBM will honor original 3 year factory warranty as long as monitor is not physically damaged. One of our IBM monitors was refurbished, with a screen defect. IBM replaced the monitor. Consult with IBM regarding the warranty, before buying used monitor. Have monitor serial number when calling IBM. These monitors are still available, mostly on e-bay, and through some internet outlets. Could be even new in box, but not from a regular source.
Is anybody at IBM reading this message? Please comment on a future availability of equal or better display monitor.
See T221 in EM application at www-1.ibm.com/industries/healthcare/doc/content/bin/ls_t221_enhancing_electr on_1.pdf
Vitaly Feingold SIA 2773 Heath Lane Duluth, GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- } From: "Mardinly, John" {john.mardinly-at-intel.com} To: "Don Chernoff at ASM" {donc-at-asmicro.com} ; "Microscopy List" {microscopy-at-microscopy.com} Sent: Saturday, July 02, 2005 3:53 AM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://microscopy.com/MLFormMail.html on Thursday, June 30, 2005 at 12:58:42 ---------------------------------------------------------------------------
Email: amr2w-at-virginia.edu Name: Andrew Roelant
Organization: UVa
Title-Subject: [Microscopy] [Filtered] 3-D SEM reconstruction
Question: Hi all, I know there are software programs that do 3-D images from SEM using stereo pairs, however, what if you have a more complex shaped object? I hear that there has been new software that you can use a series of tilts like TEM tomography to obtain a better reconstruction. Does anybody know about this? Any information would be very much appreciated. Thanks! -Andrew Roelant
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (srandol3-at-utk.edu) from http://www.microscopy.com/MLFormMail.html on Sunday, July 3, 2005 at 19:26:02 ---------------------------------------------------------------------------
Email: srandol3-at-utk.edu Name: Steven Randolph
Organization: university of tennessee
Title-Subject: [Microscopy] [Filtered] MListserver: Area analysis scanning
Question: Hello all,
I have a question that is really more engineering-related than imaging. My question is regarding some of the various scanning modes on Hitachi SEMs such as the S4300, S4700, and S3500N. I need to know some of the specifics about how scanning takes place in area analysis mode and reduced screen mode. In area analysis, is the beam blanked in the region outside the box, or is the pixel size reduced to accomodate the box size? I guess what I'm trying to find out is the dwell time in area analysis and reduced screen mode. I seem to recall that there is a finite settle time associated with scanning in some modes. Anyways, any input you could provide would be much appreciated!
We have a Philips SEM 515 equipped with EDX spectrometer. The spectrometer is EDAX PV 9800 system with UTW detector (freshly repaired) and control unit with its specialized computer (both hardware and software). The computer is just dying and most probably can not be repaired. I wonder if anybody tried to "upgrade" this particular system to PC compatible version. Is it possible at reasonable cost?
Regards,
Leszek
Leszek Kepinski Institute of Low Temperature and Structure Research, Polish Academy of Sciences, P.O.Box 1410, 50-950 Wroclaw, Poland e-mail: L.Kepinski-at-int.pan.wroc.pl
Well, it was wishful thinking that I could adequitely solve the problem in the first attempt. After many hours of off-line testing (and too few pints for a holiday weekend). I need to run yet another full system delivery test. You may notice a few changes in the configuration of the Email headers in this version.
The previous test basically worked, but it took far too many hours to process & verify all the addresses. I've taken a whole different aproach with this modification (after having looked at the results over night).
No need to reply to this message. Again if you have problems posting with this new version of the filters implements, then please contact me off-line. (zaluzec-at-microscopy.com)
Nestor Your Friendly Neighborhood SysOp.
Monday - July 4th, 2005
------------------------------Original Headers------------------------------ 16, 11 -- From zaluzec-at-microscopy.com Mon Jul 4 13:54:57 2005 16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 16, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j64Ist7n013713 16, 11 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jul 2005 13:54:57 -0500 16, 11 -- Mime-Version: 1.0 16, 11 -- Message-Id: {p06110449beef32f13eb5-at-[206.69.208.22]} 16, 11 -- Date: Mon, 4 Jul 2005 13:54:54 -0500 16, 11 -- To: microscopy-at-microscopy.com 16, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 16, 11 -- Subject: Administrivia: Sorry - another full system test 16, 11 -- Content-Type: text/plain; charset="us-ascii" ------------------------------------------------------------------------
The next in the FOM conference series will take place in Perth, (Western) Australia from Sunday April 9th to Wednesday April 12, 2006. Please visit for details www.FocusOnMicroscopy.org
Focus on Microscopy 2006 is the continuation of a successful conference series presenting the latest innovations in optical microscopy and its applications in biology, medicine, material science, and information storage. 3D optical imaging and related theory are important subjects for the conference. The series is as relevant now as at any time in its history as the scientific and engineering communities strive to meet the needs of a surging life sciences sector as well as respond to the sustained pressure on miniaturisation in lithography and data storage.
Also a technical exhibition will be part of the conference. In Jena well over 30 companies participated including the major microscopy companies, see FOM2005 Sponsors & Exhibitors www.focusonmicroscopy.org/2005/sponsors.html .
The 2006 meeting will be held in Perth on Australia's western seaboard. The conference will be held in the nearby port city of Fremantle, at the scenic Esplanade hotel, close to the boat harbour and Perth' famous beaches. Perth's relaxed and outdoor lifestyle should prove an ideal setting for a stimulating and enjoyable meeting - see you there!
Local organising committee: Stephen Cody Central Resource for Advanced Microscopy, Ludwig Institute for Cancer Research, Melbourne
Guy Cox Electron Microscope Unit, University of Sydney
Ewa Goldys Division of Information and Communication Sciences, Macquarie University, Sydney
Brendan Griffin Centre for Microscopy and Microanalysis, University of Western Australia, Perth
Miranda Grounds School of Anatomy and Human Biology, University of Western Australia, Perth
Ian Harper School of Biomedical Sciences, Monash University, Melbourne
David Jans Department of Biochemistry and Molecular Biology, Monash University, Melbourne
Min Gu Centre for Microphotonics, Swinburne University, Melbourne
John Kuo Centre for Microscopy and Microanalysis, University of Western Australia, Perth
Keith Nugent School of Physics, University of Melbourne
Paul Rigby Biomedical Imaging and Analysis Facility, University of Western Australia, Perth
Alpha Yap Institute for Biomolecular Science, University of Queensland, Brisbane
Conference topics include: * Confocal and multiphoton-excitation microscopies * Novel illumination and detection strategies - selectiveplane, extended depth of focus, 4pi, structured illumination * Fluorescence - new labels, fluorescent proteins, quantum dots, single molecule, excitation-emission spectroscopy * Time-resolved fluorescence - FRET, FRAP, FLIM, FCS * Coherent non-linear microscopies - SHG, THG, SFG, CARS * Scattering processes: Raman, light scattering spectroscopy, second harmonic * Multi-dimensional imaging * Sub-wavelength resolution - near field microscopy, total internal reflection * Laser manipulation, ablation and microdissection, photoactivation * Magnetic resonance and X-ray microscopy * Image processing and visualisation * Live cell and tissue imaging * Whole tissue imaging - optical coherence tomography, endoscopy, whole animal fluorescence * New tools in genomics, proteomics, phenomics, cytometry * Lithography and data storage
To stay informed on the program, registration and abstract submission for the conference please leave your E-mail address here www.focusonmicroscopy.org/forms/subscr_notification.html .
On behalf of the FocusOnMicroscopy society, * David Sampson, University of Western Australia * Fred Brakenhoff, University of Amsterdam, The Netherlands
Stephen H. Cody
Microscopy Manager Central Resource for Advanced Microscopy Ludwig Institute For Cancer Research PO Box 2008 Royal Melbourne Hospital Parkville Victoria 3050 Australia Tel: 61 3 9341 3155 Fax: 61 3 9341 3104 email: stephen.cody-at-ludwig.edu.au www.ludwig.edu.au/labs/confocal.html www.ludwig.edu.au/confocal
------------------------------Original Headers------------------------------ 23, 23 -- From Stephen.Cody-at-ludwig.edu.au Mon Jul 4 19:10:35 2005 23, 23 -- Received: from CL380EVS-1.ludwig.edu.au (cl380-1.ludwig.edu.au [128.250.250.24]) 23, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j650AXXm030562 23, 23 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jul 2005 19:10:34 -0500 23, 23 -- Received: from exchange.ludwig.edu.au ([172.16.2.22]) by CL380EVS-1.ludwig.edu.au with Microsoft SMTPSVC(5.0.2195.6713); 23, 23 -- Tue, 5 Jul 2005 10:10:33 +1000 23, 23 -- Content-Class: urn:content-classes:message 23, 23 -- MIME-Version: 1.0 23, 23 -- Content-Type: text/plain; 23, 23 -- charset="iso-8859-1" 23, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 23, 23 -- Subject: FOM 2006 23, 23 -- Date: Tue, 5 Jul 2005 10:10:33 +1000 23, 23 -- Message-ID: {660936C3FAE0FB47BC49BB8B60FA14EC148BFE-at-exchange.ludwig.edu.au} 23, 23 -- X-MS-Has-Attach: 23, 23 -- X-MS-TNEF-Correlator: 23, 23 -- Thread-Topic: FOM 2006 23, 23 -- thread-index: AcWA9fXZB44rSoAySq+8wRoucYuX8Q== 23, 23 -- From: "Stephen Cody" {Stephen.Cody-at-ludwig.edu.au} 23, 23 -- To: {microscopy-at-microscopy.com} 23, 23 -- X-OriginalArrivalTime: 05 Jul 2005 00:10:33.0257 (UTC) FILETIME=[F6019990:01C580F5] 23, 23 -- Content-Transfer-Encoding: 8bit 23, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j650AXXm030562
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Postdoctoral Position: Computer Vision Algorithms for Studying Biological Growth and Motility
A postdoctoral position is available to join a research project in the quantification of deformable motion in biology, with emphasis on growth and cell motility. The position is supported by an NIH-funded collaboration between Tobias I. Baskin (a biologist at Umass Amherst) and K. Palaniappan (a computer scientist at University of Missouri, Columbia). Baskin and Palaniappan have developed new software for quantifying the spatial distribution of velocity within a growing plant organ (a root). The software is called RootflowRT and the biological application is described by van der Weele et al (2003 Plant Physiology, 32:1138-1148). The software implements a novel algorithm for quantifying deformable motion that combines structure-tensor and robust-matching approaches. The project is to enhance and validate RootFlowRT, apply software engineering principles to the current code base, explore new computational algorithms, and extend the robust-tensor approach to other kinds of biological objects, in particular motile animal cells and embryos. The open position is at Amherst and involves imaging different kinds of biological object as well as enhancing the software. Applicants should have experience in some area of image processing, good programming skills, and, preferably, experience in biology.
Those interested in the position should contact Dr Baskin (email: baskin-at-bio.umass.edu), and can find further information from his web page: http://www.bio.umass.edu/biology/baskin/ and the page for RootflowRT http://meru.rnet.missouri.edu/mvl/bio_motion.
I encourage applications from anyone regardless of skin color, religion, sex, sexual orientation, or nationality.
Hi, Is anyone aware of a method using DiI for staining cells growing in a monolayer thickness, targeting the cell membrane, to obtain enough contrast to yield a binary image of the cells representing their shape/morphology?
I'm growing multi-potent cells in 24-well plates and would like to quantify their shape (and changes in shape) as they differentiate with time.
I've looked in the literature, and I've found lots of examples of DiI being used to label neurons in live tissue. However, these papers don't focus so much on quantitative morphological measurements, but more on what the neurons are ennervating. I'd like a simple method for cells growing or fixed in a plate or on a slide, for quantifying shape.
I am looking for companies or individuals providing repair services for TEMs and SEMs in the New England area. Does anyone know of a comprehensive list of contacts? Any recommendations?
Any providers or others with relevant information are welcome to contact me at the phone number listed below, or by e-mail. Thanks.
Marie
Dr. Marie E. Cantino Director, Electron Microscopy Laboratory Associate Professor of Physiology and Neurobiology University of Connecticut Unit 3242 Storrs, CT 06269-3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 5, 20 -- From marie.cantino-at-uconn.edu Wed Jul 6 13:39:00 2005 5, 20 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j66IcxI8001091 5, 20 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 6 Jul 2005 13:39:00 -0500 5, 20 -- Received: from [137.99.47.197] (d47h197.public.uconn.edu [137.99.47.197]) 5, 20 -- by mail2.uits.uconn.edu (8.11.6/8.11.6) with ESMTP id j66IcmO19281 5, 20 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 6 Jul 2005 14:38:48 -0400 5, 20 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 20 -- Message-Id: {84b91857580009a34dab5aecf6fd70a7-at-uconn.edu} 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- Reply-To: Cantino Marie {marie.cantino-at-uconn.edu} 5, 20 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 5, 20 -- Subject: EM service 5, 20 -- Date: Wed, 6 Jul 2005 14:44:15 -0400 5, 20 -- To: MSA Listserver {Microscopy-at-msa.microscopy.com} 5, 20 -- X-Mailer: Apple Mail (2.622) 5, 20 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 20 -- X-UConn-MailScanner: Found to be clean 5, 20 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
Ken Converse Quality Images Delta PA (717) 456-5491
He participates in this newsgroup.
Stu Smalinskas Metallurgist SKF Plymouth, Michigan
--- marie.cantino-at-uconn.edu wrote:
} } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- Sponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I am looking for companies or individuals providing } repair services for } TEMs and SEMs in the New England area. Does anyone } know of a } comprehensive list of contacts? Any } recommendations? } } Any providers or others with relevant information } are welcome to } contact me at the phone number listed below, or by } e-mail. Thanks. } } Marie } } Dr. Marie E. Cantino } Director, Electron Microscopy Laboratory } Associate Professor of Physiology and Neurobiology } University of Connecticut Unit 3242 } Storrs, CT 06269-3242 } Phone: 860-486-3588 } Fax: 860-486-6369 }
____________________________________________________ Sell on Yahoo! Auctions – no fees. Bid on great items. http://auctions.yahoo.com/
I'm interested in hearing how long people keep the Uranyl Acetate stain that they use in grid staining, both the stock and working solution. Currently we are using a 2% solution in 50% methanol.
How long do you think one could say that they remain fresh after making up? Presently our lab keeps the stock solution for about a month, but I never really had any literature to know one way or the other if I could keep it long.
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==============================Original Headers============================== 5, 19 -- From GBurgess-at-exchange.hsc.mb.ca Wed Jul 6 14:20:58 2005 5, 19 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j66JKw26016949 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Jul 2005 14:20:58 -0500 5, 19 -- Received: from mudslide.hsc.mb.ca (unverified [172.16.6.136]) by 5, 19 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 5, 19 -- {B0013187032-at-hscxntmx0003.hsc.mb.ca} for {Microscopy-at-microscopy.com} ;Wed, 5, 19 -- 6 Jul 2005 14:23:29 -0500 5, 19 -- Received: by mudslide.hsc.mb.ca with Internet Mail Service (5.5.2653.19)id 5, 19 -- {3F0H7VDL} ; Wed, 6 Jul 2005 14:20:52 -0500 5, 19 -- Message-ID: {00A937989100304A83A058F6C45873FF32A241-at-hscxntmx0005} 5, 19 -- Date: Wed, 6 Jul 2005 14:19:35 -0500 5, 19 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 5, 19 -- Subject: Uranyl Acetate Shelf Life 5, 19 -- To: {Microscopy-at-microscopy.com} 5, 19 -- X-Mailer: Internet Mail Service (5.5.2653.19) 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: text/plain; 5, 19 -- charset="iso-8859-1" ==============================End of - Headers==============================
We've been using multiple LCD monitors lately to increase our windows desktop size. However, in doing so I have noted that Matrox does offer a high-resolution graphics card (3840x2400) { http://www.matrox.com/mga/workstation/3dws/products/special/hr25 6.cfm } and they point to three monitors for this card something refed to as 9 MP (assume 9 mega pixel ? maybe) monitors (Viewsonic, IBM T221, and Iiayam)
NVidia Quadro FX series cards also goes to 3840x2400
ATI as a few cards which will deliver 3840 x 2400 (using multiple monitors)
IBM lists a T221 as their 9.2 megapixel monitor - but I can not find its avavilablity.
A company called Planar makes a 5-megapixel greyscale monitor. Dome C5i
www.tridentmicrosystems.co.uk lists a 28.1” 2k x 2k TFT LCD monitor is designed for traffic management.
NEC lists 2048 x 1536 as their highest resolution monitor. (But does include 10-bit greyscale as well).
Viewsonic offers a number of "Thin Edge" monitors designed to used together including stands designed to hold 2,3 or 4 monitors at once.
There is a company www.9xmedia.com that offers multimonitor solutions.
(Note: I do not sell montiors, or own stock / interests in any monitor graphics company. :-)
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } Don; } I tried to purchase an IBM T-220 9 megapixel display, but was } told by IBM that they had killed the product. This was the only display } ever marketed that could display a 2K X 2K camera image showing all of } the pixels on one screen. We still have two screens on our TEM, but need } to either display the images at half resolution, or zoom to display only } part of the image. } } John Mardinly } } -----Original Message----- } } From: Don Chernoff at ASM [mailto:donc-at-asmicro.com] } Sent: Friday, July 01, 2005 9:05 AM } To: Microscopy List } Subject: [Microscopy] Image review: Hard copy vs. PC screen } } } } ------------------------------------------------------------------------ } ------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ------- } } This post is tangential to the dye sub vs ink jet discussion. } When one needs to compare 2 or more images simultaneously, prints can } easily } be laid out on a table, but this is not so easy with on-screen display } of } digital images. One thing that we have done in our lab is to install a } second monitor at some workstations and increase the Windows desktop } size so } that it spans the two monitors. This is satisfactory for AFM images } where } the basic pixel count is 512x512 but may not be so good with other } formats. } I am curious to learn what other people do. } } When you give your customers digital images only, do you feel there is a } risk they could miss an important comparison? } } regards, } Don Chernoff } ================================== } Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com } 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 } INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & } Canada) } web: http://www.asmicro.com Fax: 317-895-5652 } [business activities: analytical services in AFM, AFM probes, } consulting, } training, } calibration and test specimens, calibration and measurement software, } used NanoScope equipment.] } } } }
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 350 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 21, 25 -- From edelmare-at-muohio.edu Wed Jul 6 16:05:08 2005 21, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 21, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j66L582x025686 21, 25 -- for {microscopy-at-Microscopy.com} ; Wed, 6 Jul 2005 16:05:08 -0500 21, 25 -- Received: from muw2k04 (muw2k04.mcs.muohio.edu [134.53.6.18]) 21, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with SMTP id j66L53TN032744 21, 25 -- for {microscopy-at-Microscopy.com} ; Wed, 6 Jul 2005 17:05:03 -0400 21, 25 -- Received: From mcsaix06.mcs.muohio.edu ([134.53.253.28]) by muw2k04 (WebShield SMTP v4.5 MR1a P0803.345); 21, 25 -- id 1120683626359; Wed, 6 Jul 2005 17:00:26 -0400 21, 25 -- Received: from emf03 ([134.53.14.119]) 21, 25 -- by mcsaix06.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j66L4wMJ013798; 21, 25 -- Wed, 6 Jul 2005 17:04:58 -0400 21, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 21, 25 -- To: "Mardinly, John" {john.mardinly-at-intel.com} , microscopy-at-Microscopy.com 21, 25 -- Date: Wed, 6 Jul 2005 17:05:02 -0400 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-type: text/plain; charset=ISO-8859-1 21, 25 -- Subject: Re: [Microscopy] RE: High Res Monitors 21, 25 -- Message-ID: {42CC0F3E.21724.1D578A6-at-localhost} 21, 25 -- Priority: normal 21, 25 -- In-reply-to: {9E1ED6A623D8CB44B4866ACF20ECDB2B06C779AA-at-scsmsx403.amr.corp.intel.com} 21, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 21, 25 -- X-Scanned-By: MIMEDefang 2.45 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id j66L582x025686 ==============================End of - Headers==============================
We have an old Zeiss EM10C, which we try to put back to the service. It come with dual channel Roentgen meter board, MOSFET. Unfortunately we do not have a circuit diagram, which will allow us to fix a problem on the board. Some one has a copy of diagram of that board?
Thanks, Vlad
==============================Original Headers============================== 5, 19 -- From uti-at-direcpc.com Thu Jul 7 06:41:13 2005 5, 19 -- Received: from a34-mta01.direcway.com (a34-mta01.direcpc.com [66.82.4.90]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j67BfD5X031659 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 06:41:13 -0500 5, 19 -- Received: from ringo.direcpc.com (dpclt034012.direcpc.com [64.157.34.12]) 5, 19 -- by a34-mta01.direcway.com 5, 19 -- (iPlanet Messaging Server 5.2 HotFix 2.05 (built Mar 3 2005)) 5, 19 -- with ESMTP id {0IJ900DYB9S9LS-at-a34-mta01.direcway.com} for 5, 19 -- microscopy-at-microscopy.com; Thu, 07 Jul 2005 07:41:09 -0400 (EDT) 5, 19 -- Date: Thu, 07 Jul 2005 07:41:48 -0400 5, 19 -- From: UTI {uti-at-direcpc.com} 5, 19 -- Subject: Zeiss EM10C - Roentgen meter board 5, 19 -- X-Sender: uti-at-pop3.direcpc.com 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- Message-id: {5.1.0.14.2.20050707073518.01e65a30-at-pop3.direcpc.com} 5, 19 -- MIME-version: 1.0 5, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 5.1 5, 19 -- Content-type: text/plain; charset=us-ascii; format=flowed 5, 19 -- Content-transfer-encoding: 7BIT ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jmjenks-at-pacbell.net) from http://microscopy.com/MLFormMail.html on Thursday, July 7, 2005 at 09:31:04 ---------------------------------------------------------------------------
Email: jmjenks-at-pacbell.net Name: Jeff Jenks
Organization: micronetpartners.com
Title-Subject: [Filtered] Senior EPMA (Electron Probe Micro Analysis) Scientist position
Silicon Valley based semiconductor equipment company seeks Senior EPMA Scientist as follows:
Mission and Duties:
- Actively participate in the development, testing and evaluation of new metrology solutions for semiconductor processing from the concept stage to prototype testing.
- Provide a broad practical and theoretical knowledge base of electron spectroscopy, X-ray optics, and electron probe micro analysis (EPMA) to assess analytical capabilities, application areas and metrology opportunities of various analysis techniques.
- Work with a team of scientists and engineers in system development, prototype testing, and design of experiments.
- Plan, perform and evaluate hardware and application feasibility studies on test setups and complete prototype systems.
Responsibilities:
- Independently perform and evaluate experiments using prototype systems
- Design experiments for EPMA concept feasibility
- Build and modify experimental test systems using hands-on skills
- Provide and maintain up-to-date knowledge of surface characterization techniques
- Model and predict electron and X-ray intensities due to particle-material interaction
- Develop, test, and evaluate measurement protocols
- Actively participate in system development and subsystem designs
Background and Education:
- MS or Ph.D. in material science, physics or related field is required; an emphasis in measurement or instrumentation is preferred
- Broad knowledge in material analysis
- Strong practical experience in electron probe analysis, preferably WDX Wavelength- Dispersive X-ray Spectroscopy or EPMA or equivalent
- Experience in semiconductor material analysis is preferred but not required
- Intimate knowledge of data reduction methods, statistical analysis and principles of particle-material interaction
- Knowledge of X-ray optics and/or X-ray optical design is desirable
- Ability to work independently and within a team of scientists and engineers
- Minimum of 2 years of practical experience in a combination of the above areas
Company offers competitive salary, stock options, benefits, and relocation assistance in a collegial work environment. Company is privately held and VC-funded with outstanding prospects for stock price appreciation in the future.
For immediate consideration please contact Jeff Jenks with your resume or CV at Jeff-at-micronetpartners.com. Or you can call Jeff at 408-850-7271 for additional details. No visa sponsorship is available. For additional openings please view www.micronetpartners.com/jobs.html.
Sorry, I seem to have scrambled the subject line of the last few postings with my latest version of the filter code. Please do not critique the person posting it was my fault.
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Lab Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov =========================================== TPMLab: http://tpm.amc.anl.gov NEESLab: http://neestpm.mcs.anl.gov MMSite: http://www.amc.anl.gov ===========================================
The box said ... "This program requires Win 95/98/NT or better..." So I bought a G3 Mac !
===========================================
==============================Original Headers============================== 7, 14 -- From zaluzec-at-aaem.amc.anl.gov Thu Jul 7 10:45:31 2005 7, 14 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 7, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j67FjVae018382 7, 14 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 10:45:31 -0500 7, 14 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105]) 7, 14 -- by aaem.amc.anl.gov (8.12.11/8.12.10) with ESMTP id j67FjT3a027583 7, 14 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 10:45:31 -0500 7, 14 -- Mime-Version: 1.0 7, 14 -- Message-Id: {p0611040cbef2fe9749d0-at-[146.139.72.105]} 7, 14 -- Date: Thu, 7 Jul 2005 10:45:29 -0500 7, 14 -- To: microscopy-at-microscopy.com 7, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 7, 14 -- Subject: Administrivia: Subject Line scrambled...sorry 7, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Colleagues
Sorry, I seem to have scrambled the subject line of the last few postings with my latest version of the filter code. Please do not critique the person posting it was my fault.
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Lab Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov =========================================== TPMLab: http://tpm.amc.anl.gov NEESLab: http://neestpm.mcs.anl.gov MMSite: http://www.amc.anl.gov ===========================================
The box said ... "This program requires Win 95/98/NT or better..." So I bought a G3 Mac !
===========================================
==============================Original Headers============================== 13, 14 -- From zaluzec-at-aaem.amc.anl.gov Thu Jul 7 10:48:26 2005 13, 14 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 13, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j67FmPWm018432 13, 14 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 10:48:25 -0500 13, 14 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105]) 13, 14 -- by aaem.amc.anl.gov (8.12.11/8.12.10) with ESMTP id j67FmO1b027590 13, 14 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 10:48:24 -0500 13, 14 -- Mime-Version: 1.0 13, 14 -- Message-Id: {p0611040dbef2ff1a687b-at-[146.139.72.105]} 13, 14 -- Date: Thu, 7 Jul 2005 10:48:23 -0500 13, 14 -- To: microscopy-at-microscopy.com 13, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 13, 14 -- Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry 13, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jmjenks-at-pacbell.net) from http://microscopy.com/MLFormMail.html on Thursday, July 7, 2005 at 09:31:04 ---------------------------------------------------------------------------
Email: jmjenks-at-pacbell.net Name: Jeff Jenks
Organization: micronetpartners.com
Title-Subject: [Filtered] Senior EPMA (Electron Probe Micro Analysis) Scientist position
Silicon Valley based semiconductor equipment company seeks Senior EPMA Scientist as follows:
Mission and Duties:
- Actively participate in the development, testing and evaluation of new metrology solutions for semiconductor processing from the concept stage to prototype testing.
- Provide a broad practical and theoretical knowledge base of electron spectroscopy, X-ray optics, and electron probe micro analysis (EPMA) to assess analytical capabilities, application areas and metrology opportunities of various analysis techniques.
- Work with a team of scientists and engineers in system development, prototype testing, and design of experiments.
- Plan, perform and evaluate hardware and application feasibility studies on test setups and complete prototype systems.
Responsibilities:
- Independently perform and evaluate experiments using prototype systems
- Design experiments for EPMA concept feasibility
- Build and modify experimental test systems using hands-on skills
- Provide and maintain up-to-date knowledge of surface characterization techniques
- Model and predict electron and X-ray intensities due to particle-material interaction
- Develop, test, and evaluate measurement protocols
- Actively participate in system development and subsystem designs
Background and Education:
- MS or Ph.D. in material science, physics or related field is required; an emphasis in measurement or instrumentation is preferred
- Broad knowledge in material analysis
- Strong practical experience in electron probe analysis, preferably WDX Wavelength- Dispersive X-ray Spectroscopy or EPMA or equivalent
- Experience in semiconductor material analysis is preferred but not required
- Intimate knowledge of data reduction methods, statistical analysis and principles of particle-material interaction
- Knowledge of X-ray optics and/or X-ray optical design is desirable
- Ability to work independently and within a team of scientists and engineers
- Minimum of 2 years of practical experience in a combination of the above areas
Company offers competitive salary, stock options, benefits, and relocation assistance in a collegial work environment. Company is privately held and VC-funded with outstanding prospects for stock price appreciation in the future.
For immediate consideration please contact Jeff Jenks with your resume or CV at Jeff-at-micronetpartners.com. Or you can call Jeff at 408-850-7271 for additional details. No visa sponsorship is available. For additional openings please view www.micronetpartners.com/jobs.html.
Sorry, I seem to have scrambled the subject line of the last few postings with my latest version of the filter code. Please do not critique the person posting it was my fault.
I have reposted at least one of the scrambled messages.
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 8, 14 -- From zaluzec-at-aaem.amc.anl.gov Thu Jul 7 10:51:16 2005 8, 14 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 8, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j67FpEIB026871 8, 14 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 10:51:15 -0500 8, 14 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105]) 8, 14 -- by aaem.amc.anl.gov (8.12.11/8.12.10) with ESMTP id j67FpEge027604 8, 14 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 10:51:14 -0500 8, 14 -- Mime-Version: 1.0 8, 14 -- Message-Id: {p0611040ebef2ffbc8e92-at-[146.139.72.105]} 8, 14 -- Date: Thu, 7 Jul 2005 10:51:13 -0500 8, 14 -- To: microscopy-at-microscopy.com 8, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 8, 14 -- Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry 8, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Greetings from Baltimore. I have a Zeiss 10 CA. Our past service company Pesto Inc. longer is in business. Does anyone know of a person or company that works on Zeiss TEMs in my area? -- Chere Petty, M.S. Manager, Keith R. Porter Imaging Facility Department of Biological Sciences University of Maryland Baltimore County (UMBC) 1000 Hilltop Circle Baltimore, MD 21250 Phone: 410-455-2296 Fax: 410-455-3875
==============================Original Headers============================== 1, 21 -- From cpetty1-at-umbc.edu Thu Jul 7 11:58:28 2005 1, 21 -- Received: from mx1out.umbc.edu (mx1out.umbc.edu [130.85.25.10]) 1, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j67GwRfI010236 1, 21 -- for {Microscopy-at-Microscopy.com} ; Thu, 7 Jul 2005 11:58:27 -0500 1, 21 -- Received: from [130.85.116.27] (biosci83pc-01.biosci.umbc.edu [130.85.116.27]) 1, 21 -- by mx1out.umbc.edu (8.12.10/8.12.10/UMBC-Central 1.1.2.1 mxout 1.2.2.3) with ESMTP id j67GwP7T009436 1, 21 -- for {Microscopy-at-Microscopy.com} ; Thu, 7 Jul 2005 12:58:26 -0400 (EDT) 1, 21 -- Message-ID: {42CD5F2B.1000908-at-umbc.edu} 1, 21 -- Date: Thu, 07 Jul 2005 12:58:19 -0400 1, 21 -- From: Chere Petty {cpetty1-at-umbc.edu} 1, 21 -- Reply-To: cpetty1-at-umbc.edu 1, 21 -- Organization: Biological Sciences - UMBC 1, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.5) Gecko/20041217 1, 21 -- X-Accept-Language: en-us, en 1, 21 -- MIME-Version: 1.0 1, 21 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 1, 21 -- Subject: TEM local service 1, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed 1, 21 -- Content-Transfer-Encoding: 7bit 1, 21 -- X-AvMilter-Key: 1120755807:ba5319d8d857ad1aef62579cf7f2f12f 1, 21 -- X-Avmilter: Message Skipped, too small ==============================End of - Headers==============================
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Dear group,
We have an old Zeiss EM10C, which we try to put back to the service. It come with dual channel Roentgen meter board, MOSFET. Unfortunately we do not have a circuit diagram, which will allow us to fix a problem on the board. Some one has a copy of diagram of that board?
Thanks, Vlad
==============================Original Headers============================== 9, 15 -- From zaluzec-at-microscopy.com Thu Jul 7 12:06:12 2005 9, 15 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 9, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j67H6BRu018004 9, 15 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 12:06:11 -0500 9, 15 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105]) 9, 15 -- by aaem.amc.anl.gov (8.12.11/8.12.10) with ESMTP id j67H6Ar2027721 9, 15 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jul 2005 12:06:11 -0500 9, 15 -- Mime-Version: 1.0 9, 15 -- X-Sender: zaluzec-at-aaem.amc.anl.gov (Unverified) 9, 15 -- Message-Id: {p06110411bef31161b153-at-[146.139.72.105]} 9, 15 -- Date: Thu, 7 Jul 2005 12:06:10 -0500 9, 15 -- To: microscopy-at-microscopy.com 9, 15 -- From: uti-at-direcpc.com (by way of Nestor J. Zaluzec) 9, 15 -- Subject: [Microscopy] Zeiss EM10C - Roentgen meter board 9, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (vincent.metzger-at-philips.com) from http://www.microscopy.com/MLFormMail.html on Thursday, July 7, 2005 at 11:26:23 ---------------------------------------------------------------------------
Email: vincent.metzger-at-philips.com Name: Vincent Metzger
Organization: philips
Title-Subject: [Filtered] Inca energy - real K-ratio ?
Question: I'm looking for thickness measurement on a stratified sample by using HT variation on SEM and a software to modelize theoricaly the interactions in the stratified sample. I need to extract real Kratio. I made some tests by passing a standart of silicium (for instance), then my sample (containig a strat of Si) and making the ratio between the 2 intensity. This work quite well, but rather time consuming. I standardized my silicium in the inca energy software, but the K-ratio displayed was totally wrong. As an example the system uses Co for quant optimization, and when you analyse the Co, a 1.6 ratio is found:crazy.
I'm wondering how the soft works, very ergonomic but rather a black box. What coefficient is applied? How to get rid of? Is there a option that can be disabled or something else?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (yaseki-at-ucsd.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 7, 2005 at 18:25:53 ---------------------------------------------------------------------------
Email: yaseki-at-ucsd.edu Name: Seki Yasuaki
Organization: University of Calfornia-San Diego
Education: Graduate College
Location: La Jolla, CA, USA
Question: Looking for vacuum schemtics, connection help for LEO 438VP SEM. The disassembled instrument has an Edwards Turbo backed by Edwards RV12 pump. The isolation block has an additional port (NW25 fitting) for connection to the specimen chamber. The microscope also has a solenoid operated PV25EK valve. I am looking for info on where this valve is to be located? a) between the block and turbo? or b) between the block and the specimen chamber? or c) elsewher? Any pictures would be of immense help as well.
My apologies if this posting a repeater- I had problems with my e-mail in the past week.
Two similar models of this monitor were available: IBM T221 and Viewsonic VP2290B. Both (I was told) used IBM display panel 22" diagonal, 3840 x 2400 pixels, 204 dpi. Both discontinued. I was also told that IBM had plans of replacing T221 with a new model as early as March 2005. I don't know whether they did yet.
We purchased several such monitors, IBM and Viewsonic, for TEM camera systems. Both models are similar in performance, except Viewsonic is a bit slower in full resolution mode, which makes no difference for static images. It's hard for a PC to run 9.2 MP frames at over 20 FPS refresh rate anyway. In fact, it is better to run 1600 x 1200 desktop most of the time and switch to 3840 x 2400 for high resolution viewing. 1600 x 1200 keeps fonts and icons size comfortable, and refresh rate is fast. The display in 3840 x 2400 mode is stunning- like looking through a window on a sunny day. You can take an eye loupe to the screen and see further detail in the image.
If you will be able to find refurbished or second hand T221 - IBM will honor original 3 year factory warranty as long as monitor is not physically damaged. One of our IBM monitors was refurbished, with a screen defect. IBM replaced the monitor. Consult with IBM regarding the warranty, before buying used monitor. Have monitor serial number when calling IBM. These monitors are still available, mostly on e-bay, and through some internet outlets. Could be even new in box, but not from a regular source.
Is anybody at IBM reading this message? Please comment on a future availability of equal or better display monitor.
See IBM paper on T221 in EM application at http://www-1.ibm.com/industries/healthcare/doc/content/bin/ls_t221_enhancing_electron_1.pdf
Vitaly Feingold SIA 2773 Heath Lane Duluth, GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: "Mardinly, John" {john.mardinly-at-intel.com} To: "Don Chernoff at ASM" {donc-at-asmicro.com} ; "Microscopy List" {microscopy-at-microscopy.com} Sent: Saturday, July 02, 2005 3:53 AM
Vitally; Lucky you for getting these monitors. At $8400 for the monitor and $2500 for the video card, not too many of us can get the funds for one, but hey, compared to the price a new TEM, why don't new TEMs come standard with at least TWO of these? I have yet to even see one. IBM told me that the T220 was the original 9 megapixel monitor. It was discontinued and then revived as the T221 with a 48 hertz refresh rate and a font handling utility so that fonts could be displayed in a readable size when the monitor was in 3840x2400 mode. What I don't understand, is that over a month after they refused to sell one to me because it was discontinued, it is still listed for sale on the IBM web site! http://www-1.ibm.com/servers/intellistation/pro/t221/index.html
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net] Sent: Thursday, July 07, 2005 11:40 PM To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John
Hi,
We have troubles with evaporation control unit BSV 202 of our BALZERS BAF 301 FE device. Does anybody have some experiences with it?
The current needed for carbon evaporation suddenly jumped so high that the 8 A fuse blown out happend. After replacing it, the current needed for carbon evaporation stays too high and exceeds the permitted current of safeguarding 8A fuse. There are no differences if we use evaporator 1 (transformer 1) or evaporator 2 (transformer 2). For carbon evaporation we use sharpenned carbon rods.
Thanking in advance for any suggestion. Oldrich ------------------------------------------------------------------------- Oldrich Benada Institute of Microbiology, Acad. Sci. CR Lab. EM Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 5, 15 -- From benada-at-biomed.cas.cz Fri Jul 8 04:23:11 2005 5, 15 -- Received: from mail.biomed.cas.cz (mail.biomed.cas.cz [147.231.40.5]) 5, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j689NBE8007185 5, 15 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jul 2005 04:23:11 -0500 5, 15 -- Received: from mail (mail.biomed.cas.cz [147.231.40.5]) 5, 15 -- by mail.biomed.cas.cz (Postfix) with SMTP id 13C9C23D7D 5, 15 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jul 2005 10:54:27 +0200 (CEST) 5, 15 -- Date: Fri, 8 Jul 2005 10:54:26 +0200 (CEST) 5, 15 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 5, 15 -- X-X-Sender: benada-at-localhost.localdomain 5, 15 -- To: Microscopy-at-microscopy.com 5, 15 -- Subject: Carbon evaporation troubles 5, 15 -- Message-ID: {Pine.LNX.4.44.0507081042510.8516-100000-at-localhost.localdomain} 5, 15 -- MIME-Version: 1.0 5, 15 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
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O.k., I'm a materials neophyte and I'm looking for some direction. I can ultramicrotome things, I can crush and fracture things, and I can physically polish things, but now I am facing a new sample area: How do I prepare thin films, inorganic, crystal and semi-crystaline grown on a hard smooth substraight (glass, Si, Mica, etc.)? Film thickness below 1-micrometer, mostly below 300nm. We would like to study the morphology of the film via SEM or TEM. EDS and EBSD would also be nice. So how do I get a clean cross-section of the thin film? Buying a dual-column/beam FIB/SEM might be a nice idea but seems a little over-kill, eh? Can someone point me in the right direction? Ion-beam milling? Ion- beam polishing? Sectioning glass or Si crystal just does not sound like a good idea.
Next are there any thoughts on buying equipment and doing this in house vs having samples preped off-campus (I acknowledge that there are some techniques and equipment that are just really tricky).
Thank you!
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 350 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 7, 23 -- From edelmare-at-muohio.edu Fri Jul 8 09:52:35 2005 7, 23 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 7, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j68EqYFA030065 7, 23 -- for {microscopy-at-Microscopy.com} ; Fri, 8 Jul 2005 09:52:34 -0500 7, 23 -- Received: from muw2k04 (muw2k04.mcs.muohio.edu [134.53.6.18]) 7, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with SMTP id j68EqWp4015970 7, 23 -- for {microscopy-at-Microscopy.com} ; Fri, 8 Jul 2005 10:52:33 -0400 7, 23 -- Received: From mcsaix06.mcs.muohio.edu ([134.53.253.28]) by muw2k04 (WebShield SMTP v4.5 MR1a P0803.345); 7, 23 -- id 1120834077984; Fri, 8 Jul 2005 10:47:57 -0400 7, 23 -- Received: from emf03 ([134.53.14.119]) 7, 23 -- by mcsaix06.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j68EqVJu075820 7, 23 -- for {microscopy-at-Microscopy.com} ; Fri, 8 Jul 2005 10:52:31 -0400 7, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 7, 23 -- To: microscopy-at-Microscopy.com 7, 23 -- Date: Fri, 8 Jul 2005 10:52:31 -0400 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-type: text/plain; charset=US-ASCII 7, 23 -- Content-transfer-encoding: 7BIT 7, 23 -- Subject: Imaging thin films 7, 23 -- Message-ID: {42CE5AEF.1499.3CAEB37-at-localhost} 7, 23 -- Priority: normal 7, 23 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 7, 23 -- X-Scanned-By: MIMEDefang 2.45 ==============================End of - Headers==============================
I have been following this thread with interest, as it affects us of course. We would like to use higher resolution monitors for our TEM cameras, but perhaps you could answer two questions for me:
1) What exactly would be the advantage of a high resolution monitor? Unless you can see the pixilation on a regular monitor, a higher resolution monitor would not show much more. To see more, the screen would also have to be much bigger. Of course, you take a magnifying glass to the monitor and see more, but that you can do with a zoom function on a regular monitor as well.
2) Is this worth around $10K or more to you?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 1:29 AM To: Mike Bode
Vitally; Lucky you for getting these monitors. At $8400 for the monitor and $2500 for the video card, not too many of us can get the funds for one, but hey, compared to the price a new TEM, why don't new TEMs come standard with at least TWO of these? I have yet to even see one. IBM told me that the T220 was the original 9 megapixel monitor. It was discontinued and then revived as the T221 with a 48 hertz refresh rate and a font handling utility so that fonts could be displayed in a readable size when the monitor was in 3840x2400 mode. What I don't understand, is that over a month after they refused to sell one to me because it was discontinued, it is still listed for sale on the IBM web site! http://www-1.ibm.com/servers/intellistation/pro/t221/index.html
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net] Sent: Thursday, July 07, 2005 11:40 PM To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John
Hi Nestor,
As of today I am getting all postings twice. Is that a problem with my subscription or a general problem?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
==============================Original Headers============================== 6, 21 -- From Mike.Bode-at-soft-imaging.net Fri Jul 8 10:08:39 2005 6, 21 -- Received: from dr-lnx1.soft-imaging.com (67.104.115.34.ptr.us.xo.net [67.104.115.34]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j68F8dRr013305 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jul 2005 10:08:39 -0500 6, 21 -- Received: from lakewood.soft-imaging.com (lakewood.soft-imaging.com [192.168.5.225]) 6, 21 -- by dr-lnx1.soft-imaging.com (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id j68F8bx00071 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jul 2005 09:08:37 -0600 6, 21 -- Received: by hq-dc2.soft-imaging.net with Internet Mail Service (5.5.2657.72) 6, 21 -- id {3GTHZ2J5} ; Fri, 8 Jul 2005 09:08:55 -0600 6, 21 -- Message-ID: {6127CE87B9BDD511B59D0001028A497D011C226A-at-hq-dc2.soft-imaging.net} 6, 21 -- From: Mike Bode {Mike.Bode-at-soft-imaging.net} 6, 21 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 21 -- Subject: For Nestor: Double posting 6, 21 -- Date: Fri, 8 Jul 2005 09:07:53 -0600 6, 21 -- Deferred-Delivery: Fri, 8 Jul 2005 09:07:00 -0600 6, 21 -- MIME-Version: 1.0 6, 21 -- X-Mailer: Internet Mail Service (5.5.2657.72) 6, 21 -- Content-Type: text/plain; 6, 21 -- charset="iso-8859-1" 6, 21 -- Content-Transfer-Encoding: 8bit 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j68F8dRr013305 ==============================End of - Headers==============================
SEM will produce morphology info whereas EBSD will produce grain, orientation and texture info. Totally different results and prep methods.
Depending on what the film material is, that would dictate how it is prepared for analysis. Silicon is easiest I think to use as a substrate since it can easily be cut into small pieces. Mount the piece and coat it then SEM image for morphology. Mount another piece and polish it with colloidal silica or alumina (again, depending on film material) down to .02u and this should work for EBSD.
FIB ablades the surface and does not produce good EBSD specimens. I've tried various plasmas and Gatan 682 and do not get good specimens for EBSD. There is probably some magic combination that I have yet to find. Thus far, mechanical polishing and slight etch with DI water seems to do the job. There are dedicated ion beam polishing tools that do nice jobs for EBSD. But unless you are going to do a lot of this, I don't think it pencils out.
Since the analysis is tops down, I don't see why you would need to section the specimen. If there is more to your situation, tell us more.
gary g.
At 07:55 AM 7/8/2005, you wrote:
} O.k., I'm a materials neophyte and I'm looking for some } direction. I can ultramicrotome things, I can crush and fracture } things, and I can physically polish things, but now I am facing a new } sample area: How do I prepare thin films, inorganic, crystal and } semi-crystaline grown on a hard smooth substraight (glass, Si, } Mica, etc.)? Film thickness below 1-micrometer, mostly below } 300nm. We would like to study the morphology of the film via SEM } or TEM. EDS and EBSD would also be nice. So how do I get a } clean cross-section of the thin film? Buying a dual-column/beam } FIB/SEM might be a nice idea but seems a little over-kill, eh? Can } someone point me in the right direction? Ion-beam milling? Ion- } beam polishing? Sectioning glass or Si crystal just does not sound } like a good idea. } } Next are there any thoughts on buying equipment and doing this in } house vs having samples preped off-campus (I acknowledge that } there are some techniques and equipment that are just really tricky). } } Thank you! } } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 350 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (biology-at-ucla.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, July 8, 2005 at 10:41:07 ---------------------------------------------------------------------------
Email: biology-at-ucla.edu Name: Eric A. Rosen
Organization: UCLA Medical Center
Title-Subject: I am trying to get a hold of Judy Murphy at the San Joaquin Delta
Question: I am trying to get a hold of Judy Murphy at the San Joaquin Delta College EM School I have a open EM position and would like to advertise it with her...
I am not quite sure what it means when you say that the resolution of the human eye is 300 DPI. I looked up an article on the web (http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which specifies the angular resolution of the human eye under optimum conditions as 1/60th of a degree. If I do my math right, the resolution is then given by:
2 x distance x tan (angle/2).
For a viewing distance of about 1m, I get a resolution of 0.3mm (which corresponds to about 220 DPI at 1m distance), which is more or less the dot pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of 0.294mm). So, I think the normal monitors are actually at the limits of the human eye's resolution. If your eyes were much better, you should be able to see the individual red, green, and blue dots from a distance of 1m.
As I said, the optimum resolution assumes certain parameters: 25 cm viewing distance, perfect lighting, etc. All changes will reduce the resolution.
But that's all mathematics. Has anybody actually compared a high res monitor and a regular monitor side by side?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 10:04 AM To: Mike Bode
Hi all,
I have been following this thread with interest, as it affects us of course. We would like to use higher resolution monitors for our TEM cameras, but perhaps you could answer two questions for me:
1) What exactly would be the advantage of a high resolution monitor? Unless you can see the pixilation on a regular monitor, a higher resolution monitor would not show much more. To see more, the screen would also have to be much bigger. Of course, you take a magnifying glass to the monitor and see more, but that you can do with a zoom function on a regular monitor as well.
2) Is this worth around $10K or more to you?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 1:29 AM To: Mike Bode
Vitally; Lucky you for getting these monitors. At $8400 for the monitor and $2500 for the video card, not too many of us can get the funds for one, but hey, compared to the price a new TEM, why don't new TEMs come standard with at least TWO of these? I have yet to even see one. IBM told me that the T220 was the original 9 megapixel monitor. It was discontinued and then revived as the T221 with a 48 hertz refresh rate and a font handling utility so that fonts could be displayed in a readable size when the monitor was in 3840x2400 mode. What I don't understand, is that over a month after they refused to sell one to me because it was discontinued, it is still listed for sale on the IBM web site! http://www-1.ibm.com/servers/intellistation/pro/t221/index.html
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net] Sent: Thursday, July 07, 2005 11:40 PM To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John
I am considering buying a paraffin microtome for our core facility. I don't personally use one much so if any knowledgeable users have recommendations on brands or models to buy (or avoid), i would welcome replies (probably best sent to me directly and not the listserver). I will be happy to keep your comments confidential. In addition, if there are special features you think are essential, i would be pleased to hear of them also. thanks, tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Mike; One meter viewing distance? Sorry, my arms are no where near that long. Fortunately my presbyopia is compensated by severe myopia. Otherwise, use reading glasses. Closer up (~1 foot?), the human eye can resolve ~80-100 microns, which is 250-300 DPI, which is why probably why 300 DPI has been a target spec for printers for a long time. The IBM T221 has a pixel pitch of 0.1245 millimeters, so those pixels are half the size of the pixels of garden variety monitors that cost less, and the way that translates into 200DPI is (25 mm/in)/(0.1245 mm/pixel)=200.8 pixels/inch. BTW, I can see the vertical mask stripes of my Sony monitor with my naked eye if I get close, and everybody in our lab could see the difference between a 400DPI Fuji Pictrograph print and a monitor display.
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Mike.Bode-at-soft-imaging.net [mailto:Mike.Bode-at-soft-imaging.net] Sent: Friday, July 08, 2005 9:46 AM To: Mardinly, John
Hello John,
I am not quite sure what it means when you say that the resolution of the human eye is 300 DPI. I looked up an article on the web (http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which specifies the angular resolution of the human eye under optimum conditions as 1/60th of a degree. If I do my math right, the resolution is then given by:
2 x distance x tan (angle/2).
For a viewing distance of about 1m, I get a resolution of 0.3mm (which corresponds to about 220 DPI at 1m distance), which is more or less the dot pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of 0.294mm). So, I think the normal monitors are actually at the limits of the human eye's resolution. If your eyes were much better, you should be able to see the individual red, green, and blue dots from a distance of 1m.
As I said, the optimum resolution assumes certain parameters: 25 cm viewing distance, perfect lighting, etc. All changes will reduce the resolution.
But that's all mathematics. Has anybody actually compared a high res monitor and a regular monitor side by side?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 10:04 AM To: Mike Bode
Hi all,
I have been following this thread with interest, as it affects us of course. We would like to use higher resolution monitors for our TEM cameras, but perhaps you could answer two questions for me:
1) What exactly would be the advantage of a high resolution monitor? Unless you can see the pixilation on a regular monitor, a higher resolution monitor would not show much more. To see more, the screen would also have to be much bigger. Of course, you take a magnifying glass to the monitor and see more, but that you can do with a zoom function on a regular monitor as well.
2) Is this worth around $10K or more to you?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 1:29 AM To: Mike Bode
Vitally; Lucky you for getting these monitors. At $8400 for the monitor and $2500 for the video card, not too many of us can get the funds for one, but hey, compared to the price a new TEM, why don't new TEMs come standard with at least TWO of these? I have yet to even see one. IBM told me that the T220 was the original 9 megapixel monitor. It was discontinued and then revived as the T221 with a 48 hertz refresh rate and a font handling utility so that fonts could be displayed in a readable size when the monitor was in 3840x2400 mode. What I don't understand, is that over a month after they refused to sell one to me because it was discontinued, it is still listed for sale on the IBM web site! http://www-1.ibm.com/servers/intellistation/pro/t221/index.html
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net] Sent: Thursday, July 07, 2005 11:40 PM To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John
Hi John,
I was actually talking about monitors. I agree with you that you normally look at prints at a shorter distance, but I think 1 m for a monitor is a reasonable distance.
And what you are saying ("BTW, I can see the vertical mask stripes of my Sony monitor with my naked eye if I get close") basically confirms my assumption: If you are NOT up close, you can't see the stripe, so the pixel size is close to resolution of the eye (at normal viewing distances). You would not be able to see or detect a further reduction in pixel size. I don't know if the width of the vertical mask is the same as a pixel. I, for one, cannot see individual green, red and blue pixels when I look at a white spot on the monitor, unless I use a magnifying glass.
Comparing printers and monitors is difficult. One is "back-illuminated", the other shows reflected light, the color gamut is different, etc. I'd be interested to hear from someone who has compared a normal LCD monitor with a high resolution LCD monitor.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 1:11 PM To: Mike Bode Cc: Listserver
Hello John,
I am not quite sure what it means when you say that the resolution of the human eye is 300 DPI. I looked up an article on the web (http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which specifies the angular resolution of the human eye under optimum conditions as 1/60th of a degree. If I do my math right, the resolution is then given by:
2 x distance x tan (angle/2).
For a viewing distance of about 1m, I get a resolution of 0.3mm (which corresponds to about 220 DPI at 1m distance), which is more or less the dot pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of 0.294mm). So, I think the normal monitors are actually at the limits of the human eye's resolution. If your eyes were much better, you should be able to see the individual red, green, and blue dots from a distance of 1m.
As I said, the optimum resolution assumes certain parameters: 25 cm viewing distance, perfect lighting, etc. All changes will reduce the resolution.
But that's all mathematics. Has anybody actually compared a high res monitor and a regular monitor side by side?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 10:04 AM To: Mike Bode
Hi all,
I have been following this thread with interest, as it affects us of course. We would like to use higher resolution monitors for our TEM cameras, but perhaps you could answer two questions for me:
1) What exactly would be the advantage of a high resolution monitor? Unless you can see the pixilation on a regular monitor, a higher resolution monitor would not show much more. To see more, the screen would also have to be much bigger. Of course, you take a magnifying glass to the monitor and see more, but that you can do with a zoom function on a regular monitor as well.
2) Is this worth around $10K or more to you?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 1:29 AM To: Mike Bode
Vitally; Lucky you for getting these monitors. At $8400 for the monitor and $2500 for the video card, not too many of us can get the funds for one, but hey, compared to the price a new TEM, why don't new TEMs come standard with at least TWO of these? I have yet to even see one. IBM told me that the T220 was the original 9 megapixel monitor. It was discontinued and then revived as the T221 with a 48 hertz refresh rate and a font handling utility so that fonts could be displayed in a readable size when the monitor was in 3840x2400 mode. What I don't understand, is that over a month after they refused to sell one to me because it was discontinued, it is still listed for sale on the IBM web site! http://www-1.ibm.com/servers/intellistation/pro/t221/index.html
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net] Sent: Thursday, July 07, 2005 11:40 PM To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John
Mike; Phil Batson reported at M&M 2004 that it was extremely useful and highly recommended. Vitaly Feingold reported this week in the Listserver that he has two. Perhaps he can also describe the experience of beholding an IBM T221.
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Mike.Bode-at-soft-imaging.net [mailto:Mike.Bode-at-soft-imaging.net] Sent: Friday, July 08, 2005 12:49 PM To: Mardinly, John
Hi John,
I was actually talking about monitors. I agree with you that you normally look at prints at a shorter distance, but I think 1 m for a monitor is a reasonable distance.
And what you are saying ("BTW, I can see the vertical mask stripes of my Sony monitor with my naked eye if I get close") basically confirms my assumption: If you are NOT up close, you can't see the stripe, so the pixel size is close to resolution of the eye (at normal viewing distances). You would not be able to see or detect a further reduction in pixel size. I don't know if the width of the vertical mask is the same as a pixel. I, for one, cannot see individual green, red and blue pixels when I look at a white spot on the monitor, unless I use a magnifying glass.
Comparing printers and monitors is difficult. One is "back-illuminated", the other shows reflected light, the color gamut is different, etc. I'd be interested to hear from someone who has compared a normal LCD monitor with a high resolution LCD monitor.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 1:11 PM To: Mike Bode Cc: Listserver
Hello John,
I am not quite sure what it means when you say that the resolution of the human eye is 300 DPI. I looked up an article on the web (http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which specifies the angular resolution of the human eye under optimum conditions as 1/60th of a degree. If I do my math right, the resolution is then given by:
2 x distance x tan (angle/2).
For a viewing distance of about 1m, I get a resolution of 0.3mm (which corresponds to about 220 DPI at 1m distance), which is more or less the dot pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of 0.294mm). So, I think the normal monitors are actually at the limits of the human eye's resolution. If your eyes were much better, you should be able to see the individual red, green, and blue dots from a distance of 1m.
As I said, the optimum resolution assumes certain parameters: 25 cm viewing distance, perfect lighting, etc. All changes will reduce the resolution.
But that's all mathematics. Has anybody actually compared a high res monitor and a regular monitor side by side?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: Mardinly, John [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 10:04 AM To: Mike Bode
Hi all,
I have been following this thread with interest, as it affects us of course. We would like to use higher resolution monitors for our TEM cameras, but perhaps you could answer two questions for me:
1) What exactly would be the advantage of a high resolution monitor? Unless you can see the pixilation on a regular monitor, a higher resolution monitor would not show much more. To see more, the screen would also have to be much bigger. Of course, you take a magnifying glass to the monitor and see more, but that you can do with a zoom function on a regular monitor as well.
2) Is this worth around $10K or more to you?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 1:29 AM To: Mike Bode
Vitally; Lucky you for getting these monitors. At $8400 for the monitor and $2500 for the video card, not too many of us can get the funds for one, but hey, compared to the price a new TEM, why don't new TEMs come standard with at least TWO of these? I have yet to even see one. IBM told me that the T220 was the original 9 megapixel monitor. It was discontinued and then revived as the T221 with a 48 hertz refresh rate and a font handling utility so that fonts could be displayed in a readable size when the monitor was in 3840x2400 mode. What I don't understand, is that over a month after they refused to sell one to me because it was discontinued, it is still listed for sale on the IBM web site! http://www-1.ibm.com/servers/intellistation/pro/t221/index.html
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net] Sent: Thursday, July 07, 2005 11:40 PM To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John
John et al.:
A few more details on what I wrote on this subject before. Lyson has been making inks and papers for photo quality ink jet printing for some time. Their new system is the "Daylight Darkroom". It is a dedicated 4 to 7 ink system (depending on your printer) for black and white printing only. I think the price includes software and inks but no printer, the system only works with certain printers. It has gotten very favorable reviews in fine art photo magazines (May/June 2005 issue of Photo Techniques, the review might be on the magazine's website (www.phototechmag.com) or on Lyson's). Lyson will send you some sample prints if you register at their website. I suspect that if someone sent Lyson a digital TEM or SEM image they might make a print of that as a sales tool. I'll bet they have no idea that there is a market for their products in the EM field. I only wish I did more than a few prints per year of parts of ground up cells. Lyson is not the only firm making inks, papers and printing systems for fine art B&W photography. Check out InkjetMall.com or MediaStreet.com. Disclaimer: I don't have any financial interest in any of the firms or products I mentioned.
Geoff
John J. Bozzola wrote:
} ------------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------------- } } } We are comparing dye sub printers versus the Fuji Pictrography PG4500. } The price differential is a staggering $22,000 versus $6,500, } respectively. It is my understanding that both produce good quality, } gray-scale prints (versus dithered, inkjet prints). Why the price } differential? I would welcome any comments, user experiences, etc. } Does anyone know the average cost per 8.5 x 11in print? } } Thank you.
--
-- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 10, 32 -- From mcauliff-at-umdnj.edu Fri Jul 8 16:04:45 2005 10, 32 -- Received: from mail01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 10, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j68L4jG7014722 10, 32 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 8 Jul 2005 16:04:45 -0500 10, 32 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 10, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 13C3FEC05E 10, 32 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 8 Jul 2005 17:04:45 -0400 (EDT) 10, 32 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 10, 32 -- by mail01.umdnj.edu (Proprietary) with ESMTP id 0239DEC057 10, 32 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 8 Jul 2005 17:04:44 -0400 (EDT) 10, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 10, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 10, 32 -- id {0IJB00E01TI1QW-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 10, 32 -- for Microscopy-at-msa.microscopy.com; Fri, 08 Jul 2005 17:04:43 -0400 (EDT) 10, 32 -- Received: from [127.0.0.1] ([10.138.2.240]) 10, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 10, 32 -- 2004)) with ESMTP id {0IJB002G2UJV7T-at-Polaris.umdnj.edu} ; Fri, 10, 32 -- 08 Jul 2005 17:04:43 -0400 (EDT) 10, 32 -- Date: Fri, 08 Jul 2005 17:04:52 -0400 10, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 10, 32 -- Subject: Re: [Microscopy] ink jet versus dye sub versus Pictrography 10, 32 -- In-reply-to: {p06110419beea0f7e2027-at-[131.230.177.142]} 10, 32 -- To: "John J. Bozzola" {bozzola-at-siu.edu} 10, 32 -- Cc: Microscopy-at-msa.microscopy.com 10, 32 -- Message-id: {42CEEA74.40601-at-umdnj.edu} 10, 32 -- MIME-version: 1.0 10, 32 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 10, 32 -- Content-transfer-encoding: 7BIT 10, 32 -- X-Accept-Language: en-us, en 10, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 10, 32 -- Gecko/20040804 Netscape/7.2 (ax) 10, 32 -- References: {p06110419beea0f7e2027-at-[131.230.177.142]} ==============================End of - Headers==============================
Richard E. Edelmann wrote: =========================================== O.k., I'm a materials neophyte and I'm looking for some direction. I can ultramicrotome things, I can crush and fracture things, and I can physically polish things, but now I am facing a new sample area: How do I prepare thin films, inorganic, crystal and semi-crystaline grown on a hard smooth substraight (glass, Si, Mica, etc.)? Film thickness below 1-micrometer, mostly below 300nm. We would like to study the morphology of the film via SEM or TEM. EDS and EBSD would also be nice. So how do I get a clean cross-section of the thin film? Buying a dual-column/beam FIB/SEM might be a nice idea but seems a little over-kill, eh? Can someone point me in the right direction? Ion-beam milling? Ion- beam polishing? Sectioning glass or Si crystal just does not sound like a good idea.
Next are there any thoughts on buying equipment and doing this in house vs having samples preped off-campus (I acknowledge that there are some techniques and equipment that are just really tricky). =============================================== Since you are already set up to do ultramicrotomy, have you considered the following:
a) Take a freshly cleaved stripping of HOPG and then do your evaporation. If you are not familiar with HOPG, see URL http://www.2spi.com/catalog/new/hopgsub.shtml In terms of smoothness, HOPG can be atomically smooth, the size of the atomically smooth areas depending on the grade of the HOPG selected.
b) Put the HOPG stripping in a flat embedding mold and then expose the coated sample, HOPG side up, to an oxygen plasma, such as in one of our Plasma Prep II plasma etchers, which will etch away the HOPG but leave your coating intact.
c) Fill the cavity containing the thin film, now with HOPG removed, with your favorite Epon 812 substitute, or SPI-Pon 812 and after curing,
d) Face off the block and diamond knife thin section the coating.
A variation on this theme would be to substitute a freshly cleaved surface of NaCl for the substrate, and after coating, the NaCl is dissolved away and the coating when dry, can be embedded as above.
You should have no difficulty getting good TEM images of the coating.
We actually prefer diamond knife thin sectioning to other methods for this kind of sample. It is not a method free of artifacts. But when they are ones that are induced by the knife edge itself, they are directional and easily recognized as being artifacts, but for the other approaches one might consider, the artifacts are not directional and therefore much more difficult to recognize.
Disclaimer: SPI Supplies offers HOPG, plasma etchers, diamond knives, and NaCl crystals so we would have a vested interest in promoting this approach relative to others.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 15, 21 -- From cgarber-at-2spi.com Fri Jul 8 17:02:14 2005 15, 21 -- Received: from diskless1.axs2000.net (mail.del.net [209.120.196.47]) 15, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j68M2Ek0023153 15, 21 -- for {microscopy-at-msa.microscopy.com} ; Fri, 8 Jul 2005 17:02:14 -0500 15, 21 -- Received: from gw.idv.net (diskless6.external [209.120.196.50]) 15, 21 -- by diskless1.axs2000.net (8.12.11/8.12.11) with ESMTP id j68M2Dmp009029; 15, 21 -- Fri, 8 Jul 2005 18:02:13 -0400 15, 21 -- X-IDV-FirstRcvd: diskless6.external [209.120.196.50] 15, 21 -- X-IDV-HELO: gw.idv.net 15, 21 -- X-Originating-IP: [81.254.170.250] 15, 21 -- From: cgarber-at-2spi.com 15, 21 -- To: microscopy-at-msa.microscopy.com 15, 21 -- Subject: Characterization of thin film coatings by TEM 15, 21 -- Date: Fri, 08 Jul 2005 22:02:14 +0000 15, 21 -- Message-ID: {20050708.UJ5.17172200-at-gw.idv.net} 15, 21 -- MIME-Version: 1.0 15, 21 -- Content-Type: text/plain; 15, 21 -- charset="iso-8859-1" 15, 21 -- Content-Transfer-Encoding: 8bit 15, 21 -- Content-Disposition: inline 15, 21 -- X-Mailer: phpGroupWare (http://www.phpgroupware.org) v 0.9.13.018 ==============================End of - Headers==============================
Oldrich Benada wrote: ================================================================= We have troubles with evaporation control unit BSV 202 of our BALZERS BAF 301 FE device. Does anybody have some experiences with it?
The current needed for carbon evaporation suddenly jumped so high that the 8 A fuse blown out happend. After replacing it, the current needed for carbon evaporation stays too high and exceeds the permitted current of safeguarding 8A fuse. There are no differences if we use evaporator 1 (transformer 1) or evaporator 2 (transformer 2). For carbon evaporation we use sharpenned carbon rods.
Thanking in advance for any suggestion. ================================================================= Have you switched sources for your "carbon" rods lately? First, some vendors offering "carbon" rods are really offering graphite rods. It is my own perception that most people who think they are using carbon rods are actually using graphite rods. Also, even among graphite rods, there is a large variation of resistivities (due to variations in density). So a common problem is that one procures the wrong kind of rods (which would have a resistivity that is out of range for their instrumental set up) so that in order to get evaporation, they exceed the instrumental parameters of their system. See URL http://2spi.com/catalog/spec_prep/carbon-graphite-rods.html for our simple "test" to judge which type of rod you are actually using.
If you have not switched rod vendors, then the second most common reason is that you are not making a sharp enough "point". Hence the tip, in order to get hot enough to evaporate carbon, draws so much current, you exceed the limits of your system. For the optimum dimensions for rod tips, see the drawings on URL http://2spi.com/catalog/spec_prep/carbon-rods.shtml
Rods can be purchased pre-sharpened from all of the major suppliers of consumables for EM laboratories, such as SPI, but if you want to make your own presharpened points, carbon rod sharpeners can be purchased as well such as are found on URL http://2spi.com/catalog/spec_prep/carbon-rod-sharpener.shtml
Disclaimer: SPI Supplies offers carbon and graphite rods as well as sharpeners.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 12, 22 -- From cgarber-at-2spi.com Sat Jul 9 03:09:02 2005 12, 22 -- Received: from smtp-wifi.orange.fr (smtp-wifi.orange.fr [194.250.131.236]) 12, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j69892Um019929 12, 22 -- for {microscopy-at-msa.microscopy.com} ; Sat, 9 Jul 2005 03:09:02 -0500 12, 22 -- Received: from ibm1x23g2abfyg (81.254.170.250) by smtp-wifi.orange.fr (7.0.031.3) 12, 22 -- id 425E7F1100039606 for microscopy-at-msa.microscopy.com; Sat, 9 Jul 2005 10:14:36 +0200 12, 22 -- Message-ID: {00d101c5845d$74ee33c0$faaafe51-at-ibm1x23g2abfyg} 12, 22 -- Reply-To: "Charles A Garber" {cgarber-at-2spi.com} 12, 22 -- From: "Charles A Garber" {cgarber-at-2spi.com} 12, 22 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 12, 22 -- Subject: carbon evaporation problem 12, 22 -- Date: Sat, 9 Jul 2005 04:08:57 -0400 12, 22 -- MIME-Version: 1.0 12, 22 -- Content-Type: text/plain; 12, 22 -- format=flowed; 12, 22 -- charset="Windows-1252"; 12, 22 -- reply-type=original 12, 22 -- Content-Transfer-Encoding: 7bit 12, 22 -- X-Priority: 3 12, 22 -- X-MSMail-Priority: Normal 12, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 12, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
It is unquestionably right that the distance of best resolution for the human eye is about 25 cm. However, I was talking about working on a monitor, and I am typically sitting at arm's length from it. I just measured my arm, and it is 75 cm. I do not work with a monitor with my eyes 25 cm from the screen. Would definitely give me headaches.
I am not trying to prove to everyone that high res monitors are useless, far from it. But I am personally not convinced that it would be worth $10,000. Considering the eye's resolution and the options of today's software, I think you can easily get what you want on a lower res monitor.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, July 08, 2005 18:50 To: Mike Bode
Barbara; YES! 10 inches is the right number! That's just slightly less than the length of my arms, and slightly more than the length of my nose, so everything I look at ends up at about 10 inches.
John Mardinly Intel
The opinions of this author do not necessarily represent the opinions on Intel Corporation.
-----Original Message----- X-from: Barbara Foster [mailto:bfoster-at-mme1.com] Sent: Friday, July 08, 2005 2:59 PM To: Mardinly, John
==============================Original Headers============================== 19, 38 -- From john.mardinly-at-intel.com Fri Jul 8 19:44:38 2005 19, 38 -- Received: from fmsfmr002.fm.intel.com (fmr14.intel.com [192.55.52.68]) 19, 38 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j690ibow032523 19, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jul 2005 19:44:38 -0500 19, 38 -- Received: from fmsfmr100.fm.intel.com (fmsfmr100.fm.intel.com [10.253.24.20]) 19, 38 -- by fmsfmr002.fm.intel.com (8.12.10/8.12.10/d: major-outer.mc,v 1.1 2004/09/17 17:50:56 root Exp $) with ESMTP id j690ibos007054; 19, 38 -- Sat, 9 Jul 2005 00:44:37 GMT 19, 38 -- Received: from fmsmsxvs043.fm.intel.com (fmsmsxvs043.fm.intel.com [132.233.42.129]) 19, 38 -- by fmsfmr100.fm.intel.com (8.12.10/8.12.10/d: major-inner.mc,v 1.2 2004/09/17 18:05:01 root Exp $) with SMTP id j690iWaC000506; 19, 38 -- Sat, 9 Jul 2005 00:44:35 GMT 19, 38 -- Received: from fmsmsx332.amr.corp.intel.com ([132.233.42.148]) 19, 38 -- by fmsmsxvs043.fm.intel.com (SAVSMTP 3.1.7.47) with SMTP id M2005070817443407650 19, 38 -- ; Fri, 08 Jul 2005 17:44:34 -0700 19, 38 -- Received: from fmsmsx311.amr.corp.intel.com ([132.233.42.214]) by fmsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 19, 38 -- Fri, 8 Jul 2005 17:44:35 -0700 19, 38 -- Received: from scsmsx402.amr.corp.intel.com ([10.3.90.16]) by fmsmsx311.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 19, 38 -- Fri, 8 Jul 2005 17:44:34 -0700 19, 38 -- Received: from scsmsx403.amr.corp.intel.com ([10.3.90.18]) by scsmsx402.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 19, 38 -- Fri, 8 Jul 2005 17:44:33 -0700 19, 38 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 19, 38 -- Content-class: urn:content-classes:message 19, 38 -- MIME-Version: 1.0 19, 38 -- Content-Type: text/plain; 19, 38 -- charset="us-ascii" 19, 38 -- Subject: RE: [Microscopy] RE: RE: RE: RE: RE: Image review: Hard copy vs. PC 19, 38 -- Date: Fri, 8 Jul 2005 17:44:32 -0700 19, 38 -- Message-ID: {9E1ED6A623D8CB44B4866ACF20ECDB2B06D40C2F-at-scsmsx403.amr.corp.intel.com} 19, 38 -- X-MS-Has-Attach: 19, 38 -- X-MS-TNEF-Correlator: 19, 38 -- Thread-Topic: [Microscopy] RE: RE: RE: RE: RE: Image review: Hard copy vs. PC 19, 38 -- Thread-Index: AcWECE7TyeyW6fSsQiW5AVr84prlVQAFmI6A 19, 38 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 19, 38 -- To: "Barbara Foster" {bfoster-at-mme1.com} 19, 38 -- Cc: "Listserver" {Microscopy-at-microscopy.com} 19, 38 -- X-OriginalArrivalTime: 09 Jul 2005 00:44:33.0970 (UTC) FILETIME=[6004B120:01C5841F] 19, 38 -- X-Scanned-By: MIMEDefang 2.44 19, 38 -- Content-Transfer-Encoding: 8bit 19, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j690ibow032523 ==============================End of - Headers==============================
==============================Original Headers============================== 32, 19 -- From Mike.Bode-at-soft-imaging.net Sat Jul 9 11:48:17 2005 32, 19 -- Received: from dr-lnx1.soft-imaging.com (67.104.115.34.ptr.us.xo.net [67.104.115.34]) 32, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j69GmH8f008366 32, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 9 Jul 2005 11:48:17 -0500 32, 19 -- Received: from lakewood.soft-imaging.com (lakewood.soft-imaging.com [192.168.5.225]) 32, 19 -- by dr-lnx1.soft-imaging.com (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id j69GmFx11662 32, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 9 Jul 2005 10:48:15 -0600 32, 19 -- Received: by hq-dc2.soft-imaging.net with Internet Mail Service (5.5.2657.72) 32, 19 -- id {3GTHZKC1} ; Sat, 9 Jul 2005 10:48:31 -0600 32, 19 -- Message-ID: {6127CE87B9BDD511B59D0001028A497D01189171-at-hq-dc2.soft-imaging.net} 32, 19 -- From: Mike Bode {Mike.Bode-at-soft-imaging.net} 32, 19 -- To: Microscopy-at-microscopy.com 32, 19 -- Subject: RE: [Microscopy] RE: RE: RE: RE: RE: RE: Image review: Ha 32, 19 -- rd copy vs. PC 32, 19 -- Date: Sat, 9 Jul 2005 10:47:25 -0600 32, 19 -- Deferred-Delivery: Sat, 9 Jul 2005 10:47:00 -0600 32, 19 -- MIME-Version: 1.0 32, 19 -- X-Mailer: Internet Mail Service (5.5.2657.72) 32, 19 -- Content-Type: text/plain ==============================End of - Headers==============================
For someone that also has far too much monitor/eye neck strain I also work at 75 cm distance. To make matters worse my desktop systems are both 3200x1200 pixels... (Dual HR Monitors) and I sometimes run with multiple virtual desktops (giving me an effective desktop area of 6400x3200 pixels. You can't display this all at once, but switching is very quick (on a Mac or Linux box)
I guess this is just a comment on technology. We are now overloaded sometimes with far too much information or we are just "parallell processing" alot now adays. Thinking back I don't see how I used to get along with the old 640x480 monitors or even worse the old VT100 text terminals.
Sigh....
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 5, 11 -- From zaluzec-at-microscopy.com Sat Jul 9 12:04:43 2005 5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 5, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j69H4gbk016217 5, 11 -- for {microscopy-at-microscopy.com} ; Sat, 9 Jul 2005 12:04:43 -0500 5, 11 -- Mime-Version: 1.0 5, 11 -- Message-Id: {p06110401bef5b13ba145-at-[206.69.208.22]} 5, 11 -- Date: Sat, 9 Jul 2005 12:04:42 -0500 5, 11 -- To: microscopy-at-microscopy.com 5, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 5, 11 -- Subject: Distance to the Monitor 5, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (normzoo-at-yahoo.com) from http://microscopy.com/MLFormMail.html on Sunday, July 10, 2005 at 04:46:26 ---------------------------------------------------------------------------
Email: normzoo-at-yahoo.com Name: Norman Shedlo
Title-Subject: [Filtered] MListserver: LM Nikon AFM microscope camera
Question: This piece of equipment seems to be relatively common. What is the usual reason for the Nikon AFM microflex shutter to stop working on this model of microscope camera?
The controller works fine and has fresh batteries.
If you can crush, fracture, and polish samples, you are well on your way to preparing samples by other means. South Bay Technology, as well as our competitors, sell complete lines of sample preparation equipment for doing what you want to do. A lot of the expertise of sample prep has been built into the instruments or is contained in the operating manuals. At the South Bay Technology web site, www.southbaytech.com, you can find a list of application notes and technical papers associated with each instrument. At our competitors' sites, you can find similar information. South Bay Technology isn't the only company that has an experienced microscopist on staff (just the best, LOL -Sorry Wolfgang and Rocco. I just couldn't resist.). There are also short courses that involve sample preparation. Ron Anderson and I are teaching a one day short course on TEM sample preparation at the M&M 2005 meeting that you might be interested in attending. We, as well as our competitors, periodically announce short courses on general EM preparation techniques and on specific techniques.
For your immediate needs, I would recommend the MicroCleave(TM) technique, (aka Small Angle Cleavage Technique). This is our model 520 kit and there is a "how to" instruction guide that I wrote that you can get at our site to learn how to do this. It is applicable to silicon, III-V and II-V compounds, SiC, sapphire, GaN, glass, and other brittle materials. It produces extremely good samples and is fairly easy to learn how to do and to teach students and doesn't require any more skills that you have already stated that you have.
For using other sample prep techniques such as dimpling, ion milling, and Tripod Polishing, you can also find specific information about these techniques at our website or you can refer to the four MRS proceedings on TEM Preparation for the Physical Sciences. The common editor in these four proceedings is Ron Anderson. I am not in my office at the moment, or I would give you the volume numbers, but I do remember three: 115, 254, and 480.
Another good way of finding out about these techniques is to find a university with an electron microscopy center that has an experienced lab director and ask for their advice. A few universities that immediately come to mind since I visited or used their facilities are Carnegie Mellon Univ, North Carolina State Univ, Ohio State Univ, Univ of Florida, Univ of California at Irvine, and Penn State Univ. I hope that I haven't slighted anyone. The people at these centers have dealt with a multitude of materials and sample preparation techniques and have a wealth of information. If they can't help you, they will know who can.
I hope this helps.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
I am not familiar with this unit. However, your description sounds like there is a short in the electrodes. Have you checked to see whether evaporated material has put a conductive path between your electrodes? Try putting a current through without the carbon rod to see if you still get a high or even a low current draw. If you do, clean the system.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-------- Original Message -------- } From: cgarber-at-2spi.com } Sent: Saturday, July 09, 2005 4:14 AM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] carbon evaporation problem } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Oldrich Benada wrote: } ================================================================= } We have troubles with evaporation control unit BSV 202 of our BALZERS BAF } 301 FE device. Does anybody have some experiences with it? } } The current needed for carbon evaporation suddenly jumped so high that the } 8 A fuse blown out happend. After replacing it, the current needed for } carbon evaporation stays too high and exceeds the permitted current of } safeguarding 8A fuse. There are no differences if we use evaporator 1 } (transformer 1) or evaporator 2 (transformer 2). } For carbon evaporation we use sharpenned carbon rods. } } Thanking in advance for any suggestion.
==============================Original Headers============================== 5, 23 -- From walck-at-southbaytech.com Mon Jul 11 02:15:11 2005 5, 23 -- Received: from smtp05.safesecureweb.com (smtp05.safesecureweb.com [209.41.179.8]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6B7FBEP002734 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2005 02:15:11 -0500 5, 23 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 5, 23 -- by smtp05.safesecureweb.com (Postfix) with ESMTP id 445AE55436F 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2005 03:15:11 -0400 (EDT) 5, 23 -- Received: from mail15.safesecureweb.com (unknown [192.168.2.180]) 5, 23 -- by smtp05.safesecureweb.com (Postfix) with ESMTP id C287A55430C 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2005 03:15:09 -0400 (EDT) 5, 23 -- MIME-Version: 1.0 5, 23 -- Date: Mon, 11 Jul 2005 03:12:28 -0400 5, 23 -- Content-Type: text/plain; 5, 23 -- charset=iso-8859-1 5, 23 -- Subject: re: [Microscopy] carbon evaporation problem 5, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 5, 23 -- Reply-To: Walck-at-southbaytech.com 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- Cc: 5, 23 -- Message-ID: {e36e4cc2faa54b4595470c6effbd0eb8-at-southbaytech.com} 5, 23 -- X-Virus-Scanned: by amavisd-new-20030616-p10 at safesecureweb.com 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6B7FBEP002734 ==============================End of - Headers==============================
Applicants are being sought for the position of Specialist Light Microscopist {jobs/SeniorMicro.htm} in the Imaging Technology Group (ITG) at the Beckman Institute at the University of Illinois at Urbana-Champaign.
Information regarding this position can be found here: http://www.itg.uiuc.edu/jobs/SeniorMicro.htm
More information on what we do at the Imaging Technology Group can be found here: http://www.itg.uiuc.edu/
Sincerely,
Jonathan M. Ekman Beckman Institute for Advanced Science and Technology, Imaging Technology Group University of Illinois at Urbana-Champaign 405 N. Mathews Avenue Urbana, IL 61801 USA Tel: 217-244-6292 Fax: 217-244-6219
==============================Original Headers============================== 6, 18 -- From ekman-at-itg.uiuc.edu Mon Jul 11 10:17:15 2005 6, 18 -- Received: from zeus.itg.uiuc.edu (zeus.itg.uiuc.edu [130.126.126.162]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6BFHFhZ019469 6, 18 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Jul 2005 10:17:15 -0500 6, 18 -- Received: from [130.126.126.232] (rollins.itg.uiuc.edu [130.126.126.232]) 6, 18 -- by zeus.itg.uiuc.edu (8.12.11/8.12.11) with ESMTP id j6BFHELO020386 6, 18 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Jul 2005 10:17:15 -0500 6, 18 -- Message-ID: {42D28D7A.4070300-at-itg.uiuc.edu} 6, 18 -- Date: Mon, 11 Jul 2005 10:17:14 -0500 6, 18 -- From: Jon Ekman {ekman-at-itg.uiuc.edu} 6, 18 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 6, 18 -- X-Accept-Language: en-us, en 6, 18 -- MIME-Version: 1.0 6, 18 -- To: Microscopy-at-Microscopy.Com 6, 18 -- Subject: LM- Light Microscopy Position - Beckman Institute at the University 6, 18 -- of Illinois at Urbana-Champaign 6, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (snydert-at-mcmaster.ca) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Monday, July 11, 2005 at 12:42:58 ---------------------------------------------------------------------------
Email: snydert-at-mcmaster.ca Name: Tom Snyder
Organization: McMaster University
Title-Subject: [Filtered] MListserver:JEOL-10S SAM Documentation
Question: Hello,
We are trying to get this Auger microscope up and running but are missing documentation about electron-optics and how they should be set. Specifically we are having trouble locating the beam.
If anyone has access to the documentation or knows what the settings should be I would appreciate any feedback. Also if you know of a good test to verify the opticas are working, that would be great info as well.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (svanhorn-at-notes.cc.sunysb.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, July 11, 2005 at 15:01:44 ---------------------------------------------------------------------------
Email: svanhorn-at-notes.cc.sunysb.edu Name: Sue Van Horn
Organization: SUNY-at-StonyBrook
Education: Graduate College
Location: Stony Brook,NY,USA
Question: has anyone ever used DAP Weldwood contact cement mixed with xylene to help in picking up serial sections???..if so how did you use it and at what dilution??? thanks sue
Sue, I don't remember if it was contact cement or some other glue, but I do remember reading (years ago) about painting the top and bottom sides of the block with a dilute "stick-um" so that as the sections were cut, they adhered to one another via the layer of sticky stuff on the edges. I never tried it, and I don't know how, if the sections adhere so well, one is supposed to separate the ribbon into pieces that will fit on a grid. I know this isn't much help, but at least you know you're not the only one who has heard of such an approach. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Sue, The use of Weldwood cement "diluted with one or two parts of commercially available contact cement thinner" (20% n-butyl acetate, 80% mineral spirits) to coat the leading and trailing block faces plus many other technique tips for serial sectioning was published by WH Fahrenbach J. Electron Microscopy Technique 1:387-398; 1984. See also EC Henry Stain Technol. 52: 59-60; 1977 who I think was first to use contact cement for this purpose
Iain Miller Department of Biological Sciences Ohio University Athens, OH 45701
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (chrisjohnrhodes-at-hotmail.com) from on Tuesday, July 12, 2005 at 10:56:47 ---------------------------------------------------------------------------
Email: chrisjohnrhodes-at-hotmail.com Name: Chris Rhodes
Organization: Syracuse University
Title-Subject: [Filtered] MListserver:
Question: I'm looking for a semi-permanent mounting medium with the best possible refractive index match to immersion oil and a cover slip (ie as close as possible to 1.515).
The best I have found so far is 1.539
It is for mounting small (less than 2mm) insect structures.
It will be used for CLSM and thus would hopefully have minimal fluorescence.
Due to numerous camera jams over the years we are a bit short on film cassettes for the Philips CM-10 and CM-100. I would appreciate your contacting me if any of you have extra cassettes that you are willing to give/sell to me.
Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
I got quite a few from our Philips 300 before we got rid of it. How many do you need? I use them to replace bad ones in our CM10 and they work fine.
Mike
Michael P. Goheen Electron Microscopy Lab Dept. of Pathology & Lab Medicine Indiana University School of Medicine Tel. 317-274-7604 Fax 317-274-5346 mgoheen-at-iupui.edu
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Tuesday, July 12, 2005 12:04 PM To: Goheen, Michael P.
Folks,
Due to numerous camera jams over the years we are a bit short on film cassettes for the Philips CM-10 and CM-100. I would appreciate your contacting me if any of you have extra cassettes that you are willing to give/sell to me.
Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
I have another one of my "back to the basics" questions that probably make people wonder how the devil I ever got into this business in the first place, but here goes anyway.
We have a client who prepares his own blocks and brings them to us for sectioning, staining, viewing, etc. One day he came into the lab with a bunch of sections on copper mesh grids which he had done immunocytochemistry with standard gold-conjugated secondaries. After viewing them, I offered him the standard advice that next time we would mount some sections on nickel or gold grids if he let us know in advance that he would be doing immunocytochemistry. He said he always used copper and asked why he should switch. My answer was something like "Umm-uhhh.....because it's always done that way" with an additional mumble about copper interfering with the labeling reaction somehow.
Now I have another client, also infinitely more savvy in chemistry that I am, asking the same question.
So I checked through our little in-house research library (again) and did some targeted Googling (again) and found that some folks say that copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use that anyway); 2) may react with PBS buffers to produce fine precipitates (but, but....doesn't that mean we should NEVER use Cu with PBS?); 3) reacts with gold colloid (no other explanation given), 4) can alter the charge distribution of the section and cause non-specific background label; and 5) may be oxidized during labelling or interfere with oxidation of chemical groups in the tissue to be labelled. Mostly people just say to use Ni or Au without stating any reasons.
Also, a glance through the literature shows that it's not uncommon to find perfectly successful immunolabelling done on copper grids.
Now, if I were determined to invade a country called Copper any or all of the above reasons could be used with little further explanation, but I'd like to know the Truth and pass it along to my admiring customers.
Any takers?
Thanks in advance.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 15, 23 -- From TindallR-at-missouri.edu Tue Jul 12 17:12:03 2005 15, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 15, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CMC2XX014275 15, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 17:12:03 -0500 15, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 15, 23 -- Tue, 12 Jul 2005 17:11:56 -0500 15, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 15, 23 -- Content-class: urn:content-classes:message 15, 23 -- MIME-Version: 1.0 15, 23 -- Content-Type: text/plain; 15, 23 -- charset="us-ascii" 15, 23 -- Subject: TEM: Immunocytochemistry and Cu grids 15, 23 -- Date: Tue, 12 Jul 2005 17:11:47 -0500 15, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE79802F3-at-UM-EMAIL09.um.umsystem.edu} 15, 23 -- X-MS-Has-Attach: 15, 23 -- X-MS-TNEF-Correlator: 15, 23 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids 15, 23 -- Thread-Index: AcWHLrKQfT52UG1vRkSz6rcfHcA8yA== 15, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 15, 23 -- To: {microscopy-at-microscopy.com} 15, 23 -- X-OriginalArrivalTime: 12 Jul 2005 22:11:56.0360 (UTC) FILETIME=[B74F4C80:01C5872E] 15, 23 -- Content-Transfer-Encoding: 8bit 15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6CMC2XX014275 ==============================End of - Headers==============================
I only ran into the "use only Ni or Au grids" rule here, in my current job, and my predecessor certainly produced some superb images showing gold labelling of TEM sections. Blissfully unaware of this rule, I had been using copper grids for all EM immunolabelling. To get good labelling of one particular structure, which is about 40 nm diameter, I used uncoated thin-bar Cu grids so I could get labelling on both sides of the section - seemed to work just fine. I'll be interested to see the responses to your question.
cheers, Rosemary
Dr. Rosemary White rosemary.white-at-csiro.au Microscopy Centre ph. 61-2-6246 5475 CSIRO Plant Industry mob. 61-0402 835 973 GPO Box 1600 fax. 61-2-6246 5334 Canberra, ACT 2601 Australia
} From: TindallR-at-missouri.edu } Reply-To: microscopy-at-microscopy.com } Date: Tue, 12 Jul 2005 17:15:38 -0500 } To: rosemary.white-at-csiro.au } Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I have another one of my "back to the basics" questions that probably } make people wonder how the devil I ever got into this business in the } first place, but here goes anyway. } } We have a client who prepares his own blocks and brings them to us for } sectioning, staining, viewing, etc. One day he came into the lab with a } bunch of sections on copper mesh grids which he had done } immunocytochemistry with standard gold-conjugated secondaries. After } viewing them, I offered him the standard advice that next time we would } mount some sections on nickel or gold grids if he let us know in advance } that he would be doing immunocytochemistry. He said he always used } copper and asked why he should switch. My answer was something like } "Umm-uhhh.....because it's always done that way" with an additional } mumble about copper interfering with the labeling reaction somehow. } } Now I have another client, also infinitely more savvy in chemistry that } I am, asking the same question. } } So I checked through our little in-house research library (again) and } did some targeted Googling (again) and found that some folks say that } copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use } that anyway); 2) may react with PBS buffers to produce fine } precipitates (but, but....doesn't that mean we should NEVER use Cu with } PBS?); 3) reacts with gold colloid (no other explanation given), 4) } can alter the charge distribution of the section and cause non-specific } background label; and 5) may be oxidized during labelling or interfere } with oxidation of chemical groups in the tissue to be labelled. Mostly } people just say to use Ni or Au without stating any reasons. } } Also, a glance through the literature shows that it's not uncommon to } find perfectly successful immunolabelling done on copper grids. } } Now, if I were determined to invade a country called Copper any or all } of the above reasons could be used with little further explanation, but } I'd like to know the Truth and pass it along to my admiring customers. } } Any takers? } } Thanks in advance. } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } } } } } ==============================Original Headers============================== } 15, 23 -- From TindallR-at-missouri.edu Tue Jul 12 17:12:03 2005 } 15, 23 -- Received: from um-exproto9.um.umsystem.edu } (um-exproto9.um.umsystem.edu [207.160.151.49]) } 15, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CMC2XX014275 } 15, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 17:12:03 -0500 } 15, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by } um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 15, 23 -- Tue, 12 Jul 2005 17:11:56 -0500 } 15, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 15, 23 -- Content-class: urn:content-classes:message } 15, 23 -- MIME-Version: 1.0 } 15, 23 -- Content-Type: text/plain; } 15, 23 -- charset="us-ascii" } 15, 23 -- Subject: TEM: Immunocytochemistry and Cu grids } 15, 23 -- Date: Tue, 12 Jul 2005 17:11:47 -0500 } 15, 23 -- Message-ID: } {BA876152E8653240BE8572E897083EE79802F3-at-UM-EMAIL09.um.umsystem.edu} } 15, 23 -- X-MS-Has-Attach: } 15, 23 -- X-MS-TNEF-Correlator: } 15, 23 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids } 15, 23 -- Thread-Index: AcWHLrKQfT52UG1vRkSz6rcfHcA8yA== } 15, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 15, 23 -- To: {microscopy-at-microscopy.com} } 15, 23 -- X-OriginalArrivalTime: 12 Jul 2005 22:11:56.0360 (UTC) } FILETIME=[B74F4C80:01C5872E] } 15, 23 -- Content-Transfer-Encoding: 8bit } 15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j6CMC2XX014275 } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 23 -- From Rosemary.White-at-csiro.au Tue Jul 12 17:53:34 2005 6, 23 -- Received: from vic-ironport-ext-out2.csiro.au (vic-ironport-ext-out2.csiro.au [150.229.64.38]) 6, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CMrXDw022373 6, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 17:53:34 -0500 6, 23 -- Received: from exgw1-cbr.nexus.csiro.au (152.83.3.66) 6, 23 -- by vic-ironport-ext-out2.csiro.au with ESMTP; 13 Jul 2005 08:53:32 +1000 6, 23 -- X-BrightmailFiltered: true 6, 23 -- X-Brightmail-Tracker: AAAAAQAAA+k= 6, 23 -- X-IronPort-AV: i="3.93,284,1114956000"; 6, 23 -- d="scan'208"; a="45339190:sNHT23254752" 6, 23 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 6, 23 -- Wed, 13 Jul 2005 08:53:31 +1000 6, 23 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 6, 23 -- Date: Wed, 13 Jul 2005 08:54:42 +1000 6, 23 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 6, 23 -- From: Rosemary White {Rosemary.White-at-csiro.au} 6, 23 -- To: {microscopy-at-microscopy.com} 6, 23 -- Message-ID: {BEFA8752.108B7%Rosemary.White-at-csiro.au} 6, 23 -- In-Reply-To: {200507122215.j6CMFcFZ019486-at-ns.microscopy.com} 6, 23 -- Mime-version: 1.0 6, 23 -- Content-type: text/plain; charset="US-ASCII" 6, 23 -- Content-transfer-encoding: 7bit 6, 23 -- X-OriginalArrivalTime: 12 Jul 2005 22:53:31.0241 (UTC) FILETIME=[865FED90:01C58734] ==============================End of - Headers==============================
As usual, I am hoping to enlist the vast knowledge and experience of the this group to solve a perplexing problem. Here is the puzzle.
We have noticed artifact elemental peaks in EDS spectra obtained with our Oxford Inca system on a Hitachi S-4700 FESEM. For example, an analysis in the center of a strip of 6 mm wide clean copper tape on an aluminum stub generates a spectrum with large peaks for copper (this is good) and a small aluminum peak (not good). Repeat the experiment with a carbon stub, and the small aluminum peak is replaced by a carbon peak.
Oddly, the phenomenon is observed only with the microscope operating in High Mag mode. The same analysis (same mag, count rate, etc.) on the copper tape in Low Mag mode detects only copper. This result is similar to what we might expect from beam scatter in a variable pressure SEM.
Anyone one out there experienced this problem? Anyone with a similar microscope willing to try the copper tape experiment to let us know if this is unique to our instrument or fundamental to the S-4700. Hitachi and Oxford are perplexed so far.
Thanks for your kind assistance.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 8, 22 -- From hanke-at-mee-inc.com Tue Jul 12 18:02:48 2005 8, 22 -- Received: from mail5.atl.registeredsite.com (mail5.atl.registeredsite.com [64.224.219.79]) 8, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CN2mBT030143 8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 18:02:48 -0500 8, 22 -- Received: from netmail.mail.registeredsite.com ([216.122.69.14]) 8, 22 -- by mail5.atl.registeredsite.com (8.12.11/8.12.8) with ESMTP id j6CN2mDA007877 8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 23:02:48 GMT 8, 22 -- Received: (qmail 61036 invoked by uid 89); 12 Jul 2005 23:09:35 -0000 8, 22 -- Received: from unknown (HELO ?192.168.1.109?) (216.43.123.204) 8, 22 -- by mail.billdalton2002.com with SMTP; 12 Jul 2005 23:09:35 -0000 8, 22 -- Message-ID: {42D44C17.4030804-at-mee-inc.com} 8, 22 -- Date: Tue, 12 Jul 2005 18:02:47 -0500 8, 22 -- From: Larry Hanke {hanke-at-mee-inc.com} 8, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 8, 22 -- X-Accept-Language: en-us, en 8, 22 -- MIME-Version: 1.0 8, 22 -- To: microscopy-at-microscopy.com 8, 22 -- Subject: Stray X-rays in FE SEM? 8, 22 -- References: {200507122213.j6CMDHwb015857-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200507122213.j6CMDHwb015857-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Randy, I had problems with Cu grids when incubating sections for long periods - overnight for instance. The copper would react with whatever was available and form green-blue salts. Not unexpected. We switched to nickel grids for a while but they charge, gold but they are delicate...by this time we had shortened the incubation times used, and tried copper again... And had no problem using coated grids and incubation times of an hour or so. Grids are floated on drops of media so that only the coated side is wetted - I don't know if that is important.
Dr SJ Stowe Facility Coordinator ANU Electron Microscopy Unit ANU CRICOS#00120C
} -----Original Message----- } From: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] } Sent: Wednesday, 13 July 2005 8:12 AM } To: sally.stowe-at-anu.edu.au } Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids } } } } } } --------------------------------------------------------------- } ------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
==============================Original Headers============================== 7, 27 -- From sally.stowe-at-anu.edu.au Tue Jul 12 18:07:03 2005 7, 27 -- Received: from mail.rsbs.anu.edu.au (rsbspc309.anu.edu.au [150.203.72.70]) 7, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CN729f005441 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 18:07:03 -0500 7, 27 -- Received: from rsbs2262 (rsbs2262.rsbs.anu.edu.au [150.203.36.144]) 7, 27 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP id A10A1F4408C 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 09:06:25 +1000 (EST) 7, 27 -- Reply-To: {Sally.Stowe-at-anu.edu.au} 7, 27 -- From: "Sally Stowe" {Sally.Stowe-at-anu.edu.au} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- Subject: RE: [Microscopy] TEM: Immunocytochemistry and Cu grids 7, 27 -- Date: Wed, 13 Jul 2005 09:06:26 +1000 7, 27 -- Message-ID: {006201c58736$549b3a10$9024cb96-at-rsbs.anu.edu.au} 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="US-ASCII" 7, 27 -- X-Priority: 3 (Normal) 7, 27 -- X-MSMail-Priority: Normal 7, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 7, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1409 7, 27 -- In-Reply-To: {200507122212.j6CMCCCH014469-at-ns.microscopy.com} 7, 27 -- Importance: Normal 7, 27 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information 7, 27 -- X-RSBS-MailScanner: Found to be clean 7, 27 -- X-MailScanner-From: sally.stowe-at-anu.edu.au 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6CN729f005441 ==============================End of - Headers==============================
What is the analytical WD for EDS in this SEM? What WD are you using?
gary g.
At 04:04 PM 7/12/2005, you wrote:
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==============================Original Headers============================== 8, 18 -- From gary-at-gaugler.com Tue Jul 12 18:30:49 2005 8, 18 -- Received: from smtp2.mc.surewest.net (smtp2.mc.surewest.net [66.60.130.51]) 8, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6CNUn3P013376 8, 18 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 18:30:49 -0500 8, 18 -- Received: (s3-16861); Tue, 12 Jul 2005 16:30:48 -0700 8, 18 -- Received: from unknown (66.60.171.211) 8, 18 -- by smtp2.mc.surewest.net (s3-smtpd/0.90-beta3) with SMTP; Tue, 12 Jul 2005 16:30:47 -0700 8, 18 -- Message-Id: {6.2.0.14.2.20050712163005.02861a30-at-mail.calweb.com} 8, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 8, 18 -- Date: Tue, 12 Jul 2005 16:30:46 -0700 8, 18 -- To: microscopy-at-microscopy.com 8, 18 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 18 -- Subject: Re: [Microscopy] Stray X-rays in FE SEM? 8, 18 -- In-Reply-To: {200507122304.j6CN4K2C001228-at-ns.microscopy.com} 8, 18 -- References: {200507122304.j6CN4K2C001228-at-ns.microscopy.com} 8, 18 -- Mime-Version: 1.0 8, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 18 -- X-TST: smtp2.mc.surewest.net SNWK3 0.31-80 ip=66.60.171.211 ==============================End of - Headers==============================
I didn't think the ones from the EM series would work in the CM series. Our EM-400 took the larger plate cassettes with the film inserts. I thought those inserts were different from the film holders that came with the CM series but I may be wrong. If they do work than I may still have a few around as well. I gave away most of my cameras and cassettes from the EM-400 when we decommissioned it but still may have a few around. I'll check and let you know if I would like to try the ones you have.
Are you going to M&M2005?
Debby
On 7/12/05 1:40 PM, "mgoheen-at-iupui.edu" {mgoheen-at-iupui.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Debby, } } I got quite a few from our Philips 300 before we got rid of it. How many } do you need? I use them to replace bad ones in our CM10 and they work } fine. } } Mike } } Michael P. Goheen } Electron Microscopy Lab } Dept. of Pathology & Lab Medicine } Indiana University School of Medicine } Tel. 317-274-7604 } Fax 317-274-5346 } mgoheen-at-iupui.edu } } } -----Original Message----- } X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] } Sent: Tuesday, July 12, 2005 12:04 PM } To: Goheen, Michael P. } Subject: [Microscopy] Film cassettes } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Folks, } } Due to numerous camera jams over the years we are a bit short on film } cassettes for the Philips CM-10 and CM-100. I would appreciate your } contacting me if any of you have extra cassettes that you are willing to } give/sell to me. } } Thanks, } Debby } } Debby Sherman, Manager Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www3.agriculture.purdue.edu/microscopy } } } } ==============================Original } Headers============================== } 6, 20 -- From dsherman-at-purdue.edu Tue Jul 12 12:00:35 2005 } 6, 20 -- Received: from mailhub248.itcs.purdue.edu } (mailhub248.itcs.purdue.edu [128.210.5.248]) } 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6CH0ZKY027403 } 6, 20 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 } 12:00:35 -0500 } 6, 20 -- Received: from [192.168.1.104] } (cpe-24-25-218-216.san.res.rr.com [24.25.218.216]) } 6, 20 -- (authenticated bits=0) } 6, 20 -- by mailhub248.itcs.purdue.edu } (8.13.4/8.13.4/avscan-smtp) with ESMTP id j6CH0VTF031403 } 6, 20 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 } 12:00:33 -0500 } 6, 20 -- User-Agent: Microsoft-Entourage/11.0.0.040405 } 6, 20 -- Date: Tue, 12 Jul 2005 12:00:29 -0500 } 6, 20 -- Subject: Film cassettes } 6, 20 -- From: Debby Sherman {dsherman-at-purdue.edu} } 6, 20 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 6, 20 -- Message-ID: {BEF9615D.4A27%dsherman-at-purdue.edu} } 6, 20 -- Mime-version: 1.0 } 6, 20 -- Content-type: text/plain; } 6, 20 -- charset="US-ASCII" } 6, 20 -- Content-transfer-encoding: 7bit } 6, 20 -- X-PMX-Version: 4.7.1.128075 } 6, 20 -- X-PerlMx-Virus-Scanned: Yes } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 17, 26 -- From mgoheen-at-iupui.edu Tue Jul 12 13:37:06 2005 } 17, 26 -- Received: from julesburg.uits.indiana.edu } (julesburg.uits.indiana.edu [129.79.1.75]) } 17, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CIb6X8003989 } 17, 26 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 13:37:06 -0500 } 17, 26 -- Received: from iu-mssg-smtp03.ads.iu.edu } (iu-mssg-smtp03.exchange.iu.edu [129.79.1.220]) } 17, 26 -- by julesburg.uits.indiana.edu (8.12.10/8.12.10/IUPO) with ESMTP id } j6CIb4En026636 } 17, 26 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 13:37:04 -0500 } (EST) } 17, 26 -- Received: from iu-mssg-mbx01.ads.iu.edu ([129.79.1.210]) by } iu-mssg-smtp03.ads.iu.edu with Microsoft SMTPSVC(5.0.2195.6713); } 17, 26 -- Tue, 12 Jul 2005 13:37:04 -0500 } 17, 26 -- X-MIMEOLE: Produced By Microsoft Exchange V6.0.6603.0 } 17, 26 -- content-class: urn:content-classes:message } 17, 26 -- MIME-Version: 1.0 } 17, 26 -- Content-Type: text/plain; } 17, 26 -- charset="us-ascii" } 17, 26 -- Subject: RE: [Microscopy] Film cassettes } 17, 26 -- Date: Tue, 12 Jul 2005 13:37:04 -0500 } 17, 26 -- Message-ID: } {CED839BBC1F79B439C630BEBD9661A6A010CAB24-at-iu-mssg-mbx01.exchange.iu.edu} } 17, 26 -- X-MS-Has-Attach: } 17, 26 -- X-MS-TNEF-Correlator: } 17, 26 -- Thread-Topic: [Microscopy] Film cassettes } 17, 26 -- Thread-Index: AcWHA7eWMUED3onrTPq+k9A7IC4dxgADHRjg } 17, 26 -- From: "Goheen, Michael P." {mgoheen-at-iupui.edu} } 17, 26 -- To: {microscopy-at-microscopy.com} } 17, 26 -- X-OriginalArrivalTime: 12 Jul 2005 18:37:04.0891 (UTC) } FILETIME=[B3653CB0:01C58710] } 17, 26 -- Content-Transfer-Encoding: 8bit } 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j6CIb6X8003989 } ==============================End of - Headers==============================
I'm not overly familiar with the Hitachi SEMs, but is the "hi-mag" mode on your SEM an immersion lens mode? This is most likely the case if you are using an "in-lens" or "in-column" detector. If so, it is possible that the BSE are being bent around by the immersion field and striking back on the sample. Depending on the immersion field, this could be close or far from the beam. These will probably still be within the viewing angle of your EDS collimator. In their immersion lens SEMs, FEI provides an EDS mode with a weakened immersion lens field so that the BSE are bent somewhat less and strike outside the field of view of the EDS collimator.
Cheers, Henk
At 07:04 PM 7/12/2005, hanke-at-mee-inc.com wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility www.ceof.ohio-state.edu 040 Fontana Labs, 116 W. 19th Ave
==============================Original Headers============================== 10, 25 -- From colijn.1-at-osu.edu Tue Jul 12 19:12:25 2005 10, 25 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6D0CPK8028631 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 19:12:25 -0500 10, 25 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 10, 25 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x5 #31056) 10, 25 -- id {01LQJTLJP7CW9HC4MN-at-er6s1.eng.ohio-state.edu} for 10, 25 -- microscopy-at-microscopy.com; Tue, 12 Jul 2005 20:12:24 -0400 (EDT) 10, 25 -- Received: from HOC3.osu.edu 10, 25 -- (d149-67-33-174.col.wideopenwest.com [67.149.174.33]) 10, 25 -- by er6s1.eng.ohio-state.edu (PMDF V6.2-1x5 #31056) 10, 25 -- with ESMTPA id {01LQJTLISNP09H2SL1-at-er6s1.eng.ohio-state.edu} for 10, 25 -- microscopy-at-microscopy.com; Tue, 12 Jul 2005 20:12:24 -0400 (EDT) 10, 25 -- Date: Tue, 12 Jul 2005 20:12:10 -0400 10, 25 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 25 -- Subject: Re: [Microscopy] Stray X-rays in FE SEM? 10, 25 -- In-reply-to: {200507122304.j6CN4Fdd000963-at-ns.microscopy.com} 10, 25 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 25 -- To: microscopy-at-microscopy.com 10, 25 -- Message-id: {6.2.0.14.2.20050712200205.01ea9018-at-mail.er6.eng.ohio-state.edu} 10, 25 -- MIME-version: 1.0 10, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 10, 25 -- Content-type: text/plain; charset=us-ascii; format=flowed 10, 25 -- X-Env-From: auth/colijn.1-at-osu.edu 10, 25 -- References: {200507122304.j6CN4Fdd000963-at-ns.microscopy.com} ==============================End of - Headers==============================
The CM series used both the 36 and 56 sheet film boxes. We have both an early CM12 which used the 2-part film carriers (36 exposure box; same as our old EM400) and a CM200 with the thinner single piece film carriers (56 exposure box). I don't know if the EM300 used the same film carriers as the EM400 series since the camera boxes in the EM300 we had (years ago) were different. The EM400 and later Philips/FEI scopes use the single "behind the column" film box assembly
I believe the issue is more the film box than the microscope. ...and more specifically, the film transport tray since the 2 carriers are different thicknesses. The boxes have the same outside dimensions and are (I believe) interchangeable. In other words, I could take my old 36 exposure film box, drop it right into my CM200 and only need to change the film stock number. CAUTION -- I haven't actually tested this since I haven't felt motivated to reduce the number of exposures in my microscope!
Cheers, Henk
At 08:10 PM 7/12/2005, dsherman-at-purdue.edu wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility www.ceof.ohio-state.edu 040 Fontana Labs, 116 W. 19th Ave
==============================Original Headers============================== 9, 25 -- From colijn.1-at-osu.edu Tue Jul 12 19:24:11 2005 9, 25 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6D0OB9s004623 9, 25 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 19:24:11 -0500 9, 25 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 9, 25 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x5 #31056) 9, 25 -- id {01LQJU14I6PC9H5VYA-at-er6s1.eng.ohio-state.edu} for 9, 25 -- microscopy-at-microscopy.com; Tue, 12 Jul 2005 20:24:11 -0400 (EDT) 9, 25 -- Received: from HOC3.osu.edu 9, 25 -- (d149-67-33-174.col.wideopenwest.com [67.149.174.33]) 9, 25 -- by er6s1.eng.ohio-state.edu (PMDF V6.2-1x5 #31056) 9, 25 -- with ESMTPA id {01LQJU13LJA89H2SL1-at-er6s1.eng.ohio-state.edu} for 9, 25 -- microscopy-at-microscopy.com; Tue, 12 Jul 2005 20:24:10 -0400 (EDT) 9, 25 -- Date: Tue, 12 Jul 2005 20:24:04 -0400 9, 25 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 25 -- Subject: Re: [Microscopy] Film cassettes 9, 25 -- In-reply-to: {200507130010.j6D0A1MX024791-at-ns.microscopy.com} 9, 25 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 25 -- To: microscopy-at-microscopy.com 9, 25 -- Message-id: {6.2.0.14.2.20050712201248.01eb33d0-at-mail.er6.eng.ohio-state.edu} 9, 25 -- MIME-version: 1.0 9, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 9, 25 -- Content-type: text/plain; charset=us-ascii; format=flowed 9, 25 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 25 -- References: {200507130010.j6D0A1MX024791-at-ns.microscopy.com} ==============================End of - Headers==============================
We used Cu grids for many years. Normally we use formvar + carbon coated grids for ICC since we have a lot less loss of sections. We also tend to do long incubations ...usually overnight. This is time efficient and also lets us use highly diluted antibody so also minimize background due to cross-reactions from contaminants in polyclonal antibodies.
Occasionally we would have a reaction due to copper oxidizing with resultant green solution. I attributed this to salts or traces of Tween 20 in the buffer and breaks in the coating exposing the Cu. As far as I can see, this is the only reason for not using Cu grids if you use coated grids. We have switched for the most part to using coated Ni grids to avoid this problem.
On the other hand, occasionally we need to do a double labeling using uncoated grids and both sides of the sections. I would hesitate to use Cu for this and prefer Ni. Gold would work but is both more expensive and more delicate than Ni grids.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
On 7/12/05 5:15 PM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I have another one of my "back to the basics" questions that probably } make people wonder how the devil I ever got into this business in the } first place, but here goes anyway. } } We have a client who prepares his own blocks and brings them to us for } sectioning, staining, viewing, etc. One day he came into the lab with a } bunch of sections on copper mesh grids which he had done } immunocytochemistry with standard gold-conjugated secondaries. After } viewing them, I offered him the standard advice that next time we would } mount some sections on nickel or gold grids if he let us know in advance } that he would be doing immunocytochemistry. He said he always used } copper and asked why he should switch. My answer was something like } "Umm-uhhh.....because it's always done that way" with an additional } mumble about copper interfering with the labeling reaction somehow. } } Now I have another client, also infinitely more savvy in chemistry that } I am, asking the same question. } } So I checked through our little in-house research library (again) and } did some targeted Googling (again) and found that some folks say that } copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use } that anyway); 2) may react with PBS buffers to produce fine } precipitates (but, but....doesn't that mean we should NEVER use Cu with } PBS?); 3) reacts with gold colloid (no other explanation given), 4) } can alter the charge distribution of the section and cause non-specific } background label; and 5) may be oxidized during labelling or interfere } with oxidation of chemical groups in the tissue to be labelled. Mostly } people just say to use Ni or Au without stating any reasons. } } Also, a glance through the literature shows that it's not uncommon to } find perfectly successful immunolabelling done on copper grids. } } Now, if I were determined to invade a country called Copper any or all } of the above reasons could be used with little further explanation, but } I'd like to know the Truth and pass it along to my admiring customers. } } Any takers? } } Thanks in advance. } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } } } } } ==============================Original Headers============================== } 15, 23 -- From TindallR-at-missouri.edu Tue Jul 12 17:12:03 2005 } 15, 23 -- Received: from um-exproto9.um.umsystem.edu } (um-exproto9.um.umsystem.edu [207.160.151.49]) } 15, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CMC2XX014275 } 15, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 17:12:03 -0500 } 15, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by } um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 15, 23 -- Tue, 12 Jul 2005 17:11:56 -0500 } 15, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 15, 23 -- Content-class: urn:content-classes:message } 15, 23 -- MIME-Version: 1.0 } 15, 23 -- Content-Type: text/plain; } 15, 23 -- charset="us-ascii" } 15, 23 -- Subject: TEM: Immunocytochemistry and Cu grids } 15, 23 -- Date: Tue, 12 Jul 2005 17:11:47 -0500 } 15, 23 -- Message-ID: } {BA876152E8653240BE8572E897083EE79802F3-at-UM-EMAIL09.um.umsystem.edu} } 15, 23 -- X-MS-Has-Attach: } 15, 23 -- X-MS-TNEF-Correlator: } 15, 23 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids } 15, 23 -- Thread-Index: AcWHLrKQfT52UG1vRkSz6rcfHcA8yA== } 15, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 15, 23 -- To: {microscopy-at-microscopy.com} } 15, 23 -- X-OriginalArrivalTime: 12 Jul 2005 22:11:56.0360 (UTC) } FILETIME=[B74F4C80:01C5872E] } 15, 23 -- Content-Transfer-Encoding: 8bit } 15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j6CMC2XX014275 } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 21 -- From dsherman-at-purdue.edu Tue Jul 12 19:46:33 2005 12, 21 -- Received: from mailhub248.itcs.purdue.edu (mailhub248.itcs.purdue.edu [128.210.5.248]) 12, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6D0kWdK013786 12, 21 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 19:46:32 -0500 12, 21 -- Received: from [192.168.1.104] (cpe-24-25-218-216.san.res.rr.com [24.25.218.216]) 12, 21 -- (authenticated bits=0) 12, 21 -- by mailhub248.itcs.purdue.edu (8.13.4/8.13.4/avscan-smtp) with ESMTP id j6D0kUI5025545 12, 21 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 19:46:31 -0500 12, 21 -- User-Agent: Microsoft-Entourage/11.0.0.040405 12, 21 -- Date: Tue, 12 Jul 2005 19:46:28 -0500 12, 21 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 12, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 12, 21 -- To: "message to: MSA list " {microscopy-at-microscopy.com} 12, 21 -- Message-ID: {BEF9CE94.4A5B%dsherman-at-purdue.edu} 12, 21 -- In-Reply-To: {200507122215.j6CMFI6D018851-at-ns.microscopy.com} 12, 21 -- Mime-version: 1.0 12, 21 -- Content-type: text/plain; 12, 21 -- charset="US-ASCII" 12, 21 -- Content-transfer-encoding: 7bit 12, 21 -- X-PMX-Version: 4.7.1.128075 12, 21 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
The film holders for the Philips EM400 and the CM series are the same. The same holders can be used in the Philips EM201,EM300, EM301 . With the inserts you load cut film without you load glass plates. ----- Original Message ----- X-from: {dsherman-at-purdue.edu} To: {amtecss-at-earthlink.net} Sent: Tuesday, July 12, 2005 8:08 PM
randy
you don't say what your client's stuff looked like. that would help.
had a former director, before i grew up and went back to school - or was that truly growing up? anyway, out of nickel grids, not to mention formvar-nickel. she wanted IEM done. then! forthwith! no delay! do not put it of! threat of discipline if not in her hands that afternoon!! etc. insisted i use copper. this was also on formvar coated grids. not pretty, quite nasty, large semicrystals of junk all over. really did not look good after the second and third try.
there were several possible problems. first, we spun things onto the grid, 30 minutes in an air driven ultracentrifuge. so the copper back surface was guaranteed to get wet and react with the buffer. or perhaps it was the buffer she had things in, i never could get a straight answer from her on that one.
recently i did a long ultracentrifuge run to load some 10S particles onto a formvar-copper grid. the grids also reacted with the phosphate buffer over the 4 hour spin time. again, not a pretty picture - i had to go back to my collaborator and get fresh material so we could put it onto formvar-nickel grids. wonderful gentleman. much more mature. real pleasure to collaborate with.
having said that, i read what rosemary white, sally stowe and debbie sherman have said. i make no pretense about my opinion on dogma (dogma-schmogma, it ain't gospel until i try it and prove the fact to me personally). give it a go and let the rest of us try out to see what happens. perhaps i will even try it in my overworked 10 finger lab. but after the two molecular experiments i need to do in order get that paper of mine accepted on resubmission, and the 7 other collaborators' projects, the looking at micrographs from 2 outside sources with questions, etc. all right, i exagerate, it's not quite that bad but i really do have to get back to one person about his results before i go on holiday tomorrow.
give it a shot and let us know
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 10, 18 -- From paul_hazelton-at-umanitoba.ca Tue Jul 12 22:55:26 2005 10, 18 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 10, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6D3tQXP031283 10, 18 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 22:55:26 -0500 10, 18 -- Received: from umanitoba.ca (cvx-045.cc.umanitoba.ca [130.179.152.108]) 10, 18 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id j6D3tH1b004399 10, 18 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 22:55:23 -0500 (CDT) 10, 18 -- Message-ID: {42D4924F.EE51DAB5-at-umanitoba.ca} 10, 18 -- Date: Tue, 12 Jul 2005 23:02:30 -0500 10, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 10, 18 -- X-Mailer: Mozilla 4.78 [en] (Win98; U) 10, 18 -- X-Accept-Language: en 10, 18 -- MIME-Version: 1.0 10, 18 -- To: microscopy-at-microscopy.com 10, 18 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 10, 18 -- References: {200507122215.j6CMF5cr018469-at-ns.microscopy.com} 10, 18 -- Content-Type: text/plain; charset=us-ascii 10, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
With magnetic field active, test stray signal as a function of WD. Same experiment with a charge sensitive sample should also be interesting, not for x-ray, but SE. response.
-----Original Message----- X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} Sent: Tuesday, July 12, 2005 11:03 PM To: "eprincipe01-at-hotmail.com" {eprincipe01-at-hotmail.com}
Dear Listers:
As usual, I am hoping to enlist the vast knowledge and experience of the this group to solve a perplexing problem. Here is the puzzle.
We have noticed artifact elemental peaks in EDS spectra obtained with our Oxford Inca system on a Hitachi S-4700 FESEM. For example, an analysis in the center of a strip of 6 mm wide clean copper tape on an aluminum stub generates a spectrum with large peaks for copper (this is good) and a small aluminum peak (not good). Repeat the experiment with a carbon stub, and the small aluminum peak is replaced by a carbon peak.
Oddly, the phenomenon is observed only with the microscope operating in High Mag mode. The same analysis (same mag, count rate, etc.) on the copper tape in Low Mag mode detects only copper. This result is similar to what we might expect from beam scatter in a variable pressure SEM.
Anyone one out there experienced this problem? Anyone with a similar microscope willing to try the copper tape experiment to let us know if this is unique to our instrument or fundamental to the S-4700. Hitachi and Oxford are perplexed so far.
Thanks for your kind assistance.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 8, 22 -- From hanke-at-mee-inc.com Tue Jul 12 18:02:48 2005 8, 22 -- Received: from mail5.atl.registeredsite.com (mail5.atl.registeredsite.com [64.224.219.79]) 8, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CN2mBT030143 8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 18:02:48 -0500 8, 22 -- Received: from netmail.mail.registeredsite.com ([216.122.69.14]) 8, 22 -- by mail5.atl.registeredsite.com (8.12.11/8.12.8) with ESMTP id j6CN2mDA007877 8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 23:02:48 GMT 8, 22 -- Received: (qmail 61036 invoked by uid 89); 12 Jul 2005 23:09:35 -0000 8, 22 -- Received: from unknown (HELO ?192.168.1.109?) (216.43.123.204) 8, 22 -- by mail.billdalton2002.com with SMTP; 12 Jul 2005 23:09:35 -0000 8, 22 -- Message-ID: {42D44C17.4030804-at-mee-inc.com} 8, 22 -- Date: Tue, 12 Jul 2005 18:02:47 -0500 8, 22 -- From: Larry Hanke {hanke-at-mee-inc.com} 8, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 8, 22 -- X-Accept-Language: en-us, en 8, 22 -- MIME-Version: 1.0 8, 22 -- To: microscopy-at-microscopy.com 8, 22 -- Subject: Stray X-rays in FE SEM? 8, 22 -- References: {200507122213.j6CMDHwb015857-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200507122213.j6CMDHwb015857-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 34 -- From eprincipe01-at-hotmail.com Tue Jul 12 23:22:57 2005 19, 34 -- Received: from hotmail.com (bay0-dav-040.bay0.hotmail.com [64.4.60.62]) 19, 34 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6D4Mvqm006821 19, 34 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 23:22:57 -0500 19, 34 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 19, 34 -- Tue, 12 Jul 2005 21:22:54 -0700 19, 34 -- Message-ID: {BAY0-DAV-0403BFF608A882A9ABCE754B1DE0-at-phx.gbl} 19, 34 -- Received: from 207.68.174.36 by BAY0-DAV-040.phx.gbl with DAV; 19, 34 -- Wed, 13 Jul 2005 04:22:53 +0000 19, 34 -- X-Originating-IP: [207.68.174.36] 19, 34 -- X-Originating-Email: [eprincipe01-at-hotmail.com] 19, 34 -- X-Sender: eprincipe01-at-hotmail.com 19, 34 -- From: "Edward Principe" {eprincipe01-at-hotmail.com} 19, 34 -- Cc: 19, 34 -- Date: Tue, 12 Jul 2005 21:22:54 -0700 19, 34 -- thread-index: AcWHYbjkNc3aUdtgRZWNu5tAwUFXVg== 19, 34 -- To: {microscopy-at-microscopy.com} 19, 34 -- Thread-Topic: [Microscopy] Stray X-rays in FE SEM? 19, 34 -- MIME-Version: 1.0 19, 34 -- Content-Type: text/plain; 19, 34 -- charset="iso-8859-1" 19, 34 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1106 19, 34 -- X-Message-Status: n 19, 34 -- X-SID-PRA: hanke-at-mee-inc.com 19, 34 -- X-SID-Result: TempError 19, 34 -- X-Message-Info: JGTYoYF78jGeUUdCkFIoKJh+2+AQwGIRJvW2yuJm+a0= 19, 34 -- Received: from ns.microscopy.com ([206.69.208.10]) by mc7-f18.hotmail.com with Microsoft SMTPSVC(6.0.3790.211); Tue, 12 Jul 2005 16:03:33 -0700,from ns.microscopy.com (localhost.localdomain [127.0.0.1]) by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CN3XLw032069 for {eprincipe01-at-hotmail.com} ; Tue, 12 Jul 2005 18:03:33 -0500,(from mail-at-localhost) by ns.microscopy.com (8.12.11/8.12.10/Submit) id j6CN3Xks032067; Tue, 12 Jul 2005 18:03:33 -0500 19, 34 -- X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} 19, 34 -- Subject: RE: [Microscopy] Stray X-rays in FE SEM? 19, 34 -- Errors-to: MicroscopyListSpamFilter-at-microscopy.com 19, 34 -- X-lewp: MicroscopyListSpam NAGS 19, 34 -- X-OriginalArrivalTime: 12 Jul 2005 23:03:33.0917 (UTC) FILETIME=[ED98FCD0:01C58735] 19, 34 -- Content-Transfer-Encoding: 8bit 19, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6D4Mvqm006821 ==============================End of - Headers==============================
I had a bad experience with formvar-copper grids in immunogold experiment many years ago when incubating primary antibody overnight and the rest done on the following morning. I am using Nickel grids now -- no more problems.
Ann Fook Yang EM Unit/ Unite EM Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 613-759-1638 Facsimile/Télécopieur: 613-759-1701 960 Carling Ave/960 Boul Carling Ottawa,Ontario/Ottawa, Ontario Canada K1A 0C6 yanga-at-agr.gc.ca
Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: Tuesday, July 12, 2005 6:55 PM To: Yang, Ann-Fook
Dear Randy,
I only ran into the "use only Ni or Au grids" rule here, in my current job, and my predecessor certainly produced some superb images showing gold labelling of TEM sections. Blissfully unaware of this rule, I had been using copper grids for all EM immunolabelling. To get good labelling of one particular structure, which is about 40 nm diameter, I used uncoated thin-bar Cu grids so I could get labelling on both sides of the section - seemed to work just fine. I'll be interested to see the responses to your question.
cheers, Rosemary
Dr. Rosemary White rosemary.white-at-csiro.au Microscopy Centre ph. 61-2-6246 5475 CSIRO Plant Industry mob. 61-0402 835 973 GPO Box 1600 fax. 61-2-6246 5334 Canberra, ACT 2601 Australia
} From: TindallR-at-missouri.edu } Reply-To: microscopy-at-microscopy.com } Date: Tue, 12 Jul 2005 17:15:38 -0500 } To: rosemary.white-at-csiro.au } Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I have another one of my "back to the basics" questions that probably } make people wonder how the devil I ever got into this business in the } first place, but here goes anyway. } } We have a client who prepares his own blocks and brings them to us for } sectioning, staining, viewing, etc. One day he came into the lab with a } bunch of sections on copper mesh grids which he had done } immunocytochemistry with standard gold-conjugated secondaries. After } viewing them, I offered him the standard advice that next time we would } mount some sections on nickel or gold grids if he let us know in advance } that he would be doing immunocytochemistry. He said he always used } copper and asked why he should switch. My answer was something like } "Umm-uhhh.....because it's always done that way" with an additional } mumble about copper interfering with the labeling reaction somehow. } } Now I have another client, also infinitely more savvy in chemistry that } I am, asking the same question. } } So I checked through our little in-house research library (again) and } did some targeted Googling (again) and found that some folks say that } copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use } that anyway); 2) may react with PBS buffers to produce fine } precipitates (but, but....doesn't that mean we should NEVER use Cu with } PBS?); 3) reacts with gold colloid (no other explanation given), 4) } can alter the charge distribution of the section and cause non-specific } background label; and 5) may be oxidized during labelling or interfere } with oxidation of chemical groups in the tissue to be labelled. Mostly } people just say to use Ni or Au without stating any reasons. } } Also, a glance through the literature shows that it's not uncommon to } find perfectly successful immunolabelling done on copper grids. } } Now, if I were determined to invade a country called Copper any or all } of the above reasons could be used with little further explanation, but } I'd like to know the Truth and pass it along to my admiring customers. } } Any takers? } } Thanks in advance. } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } } } } } ==============================Original Headers============================== } 15, 23 -- From TindallR-at-missouri.edu Tue Jul 12 17:12:03 2005 } 15, 23 -- Received: from um-exproto9.um.umsystem.edu } (um-exproto9.um.umsystem.edu [207.160.151.49]) } 15, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CMC2XX014275 } 15, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 17:12:03 -0500 } 15, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by } um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 15, 23 -- Tue, 12 Jul 2005 17:11:56 -0500 } 15, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 15, 23 -- Content-class: urn:content-classes:message } 15, 23 -- MIME-Version: 1.0 } 15, 23 -- Content-Type: text/plain; } 15, 23 -- charset="us-ascii" } 15, 23 -- Subject: TEM: Immunocytochemistry and Cu grids } 15, 23 -- Date: Tue, 12 Jul 2005 17:11:47 -0500 } 15, 23 -- Message-ID: } {BA876152E8653240BE8572E897083EE79802F3-at-UM-EMAIL09.um.umsystem.edu} } 15, 23 -- X-MS-Has-Attach: } 15, 23 -- X-MS-TNEF-Correlator: } 15, 23 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids } 15, 23 -- Thread-Index: AcWHLrKQfT52UG1vRkSz6rcfHcA8yA== } 15, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 15, 23 -- To: {microscopy-at-microscopy.com} } 15, 23 -- X-OriginalArrivalTime: 12 Jul 2005 22:11:56.0360 (UTC) } FILETIME=[B74F4C80:01C5872E] } 15, 23 -- Content-Transfer-Encoding: 8bit } 15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j6CMC2XX014275 } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 23 -- From Rosemary.White-at-csiro.au Tue Jul 12 17:53:34 2005 6, 23 -- Received: from vic-ironport-ext-out2.csiro.au (vic-ironport-ext-out2.csiro.au [150.229.64.38]) 6, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6CMrXDw022373 6, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 17:53:34 -0500 6, 23 -- Received: from exgw1-cbr.nexus.csiro.au (152.83.3.66) 6, 23 -- by vic-ironport-ext-out2.csiro.au with ESMTP; 13 Jul 2005 08:53:32 +1000 6, 23 -- X-BrightmailFiltered: true 6, 23 -- X-Brightmail-Tracker: AAAAAQAAA+k= 6, 23 -- X-IronPort-AV: i="3.93,284,1114956000"; 6, 23 -- d="scan'208"; a="45339190:sNHT23254752" 6, 23 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 6, 23 -- Wed, 13 Jul 2005 08:53:31 +1000 6, 23 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 6, 23 -- Date: Wed, 13 Jul 2005 08:54:42 +1000 6, 23 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 6, 23 -- From: Rosemary White {Rosemary.White-at-csiro.au} 6, 23 -- To: {microscopy-at-microscopy.com} 6, 23 -- Message-ID: {BEFA8752.108B7%Rosemary.White-at-csiro.au} 6, 23 -- In-Reply-To: {200507122215.j6CMFcFZ019486-at-ns.microscopy.com} 6, 23 -- Mime-version: 1.0 6, 23 -- Content-type: text/plain; charset="US-ASCII" 6, 23 -- Content-transfer-encoding: 7bit 6, 23 -- X-OriginalArrivalTime: 12 Jul 2005 22:53:31.0241 (UTC) FILETIME=[865FED90:01C58734] ==============================End of - Headers==============================
==============================Original Headers============================== 14, 29 -- From YANGA-at-AGR.GC.CA Wed Jul 13 08:24:43 2005 14, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 14, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DDOhbQ020584 14, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 08:24:43 -0500 14, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 14, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j6DDOg8Y024546 14, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 09:24:42 -0400 14, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 14, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j6DDOcOO023305 14, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 09:24:38 -0400 14, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 14, 29 -- Wed, 13 Jul 2005 09:25:17 -0400 14, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 14, 29 -- content-class: urn:content-classes:message 14, 29 -- MIME-Version: 1.0 14, 29 -- Content-Type: text/plain; 14, 29 -- charset="iso-8859-1" 14, 29 -- Subject: RE: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids 14, 29 -- Date: Wed, 13 Jul 2005 09:25:16 -0400 14, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB13553AA-at-onncrxms3.agr.gc.ca} 14, 29 -- X-MS-Has-Attach: 14, 29 -- X-MS-TNEF-Correlator: 14, 29 -- Thread-Topic: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids 14, 29 -- Thread-Index: AcWHNM6wUJRJ1yarRAuufTkDb80FgAAdzEcg 14, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} 14, 29 -- To: {microscopy-at-microscopy.com} 14, 29 -- X-OriginalArrivalTime: 13 Jul 2005 13:25:17.0171 (UTC) FILETIME=[4F237430:01C587AE] 14, 29 -- Content-Transfer-Encoding: 8bit 14, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6DDOhbQ020584 ==============================End of - Headers==============================
We have had problems with Cu grids, even Formvar coated ones. I have precoated copper grids with Parlodion by dipping, blotting and drying before use, with or with out a formvar coating. THat method seems to protect the copper from reacting with PBS or whatever. This is not original to me, but I cannot recall where I read this (among many other things I can no longer recall). I think it might have been one of those smart Swiss guys.
Greg
TindallR-at-missouri.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 23 -- From gwe-at-ufl.edu Wed Jul 13 08:39:16 2005 6, 23 -- Received: from smtp.ufl.edu (sp42en1.nerdc.ufl.edu [128.227.74.42]) 6, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DDdFCv028580 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 08:39:15 -0500 6, 23 -- Received: from [127.0.0.1] (pc2524c.dhcp.clas.ufl.edu [128.227.60.197]) 6, 23 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j6DDdCog123902 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 09:39:14 -0400 6, 23 -- Message-ID: {42D51989.90109-at-ufl.edu} 6, 23 -- Date: Wed, 13 Jul 2005 09:39:21 -0400 6, 23 -- From: Greg Erdos {gwe-at-ufl.edu} 6, 23 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 6, 23 -- X-Accept-Language: en-us, en 6, 23 -- MIME-Version: 1.0 6, 23 -- To: microscopy-at-microscopy.com 6, 23 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 6, 23 -- References: {200507122213.j6CMD58B015666-at-ns.microscopy.com} 6, 23 -- In-Reply-To: {200507122213.j6CMD58B015666-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- X-Spam-Status: hits=-0.904, required=5, tests=BAYES_30 6, 23 -- X-UFL-Spam-Status: hits=-0.904, required=5, tests=BAYES_30 6, 23 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 23 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
What's the big deal about preferring Cu to Ni anyway? Right, there is the charging problem with Nickel, but if you use anti-magnetic tweezers they are just as easy to handle than copper. And the price is really not that much more! We sometimes have to leave grids overnight or longer on solutions, so to be safe we use nickel for this. Since we don't want to make and keep separate stocks of copper and nickel, we switched entirely to nickel grids, and have had no problems with any of our applications. Am I missing something here?!!
Marc
On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Dear Listers, } } I have another one of my "back to the basics" questions that probably } make people wonder how the devil I ever got into this business in the } first place, but here goes anyway. } } We have a client who prepares his own blocks and brings them to us for } sectioning, staining, viewing, etc. One day he came into the lab with } a } bunch of sections on copper mesh grids which he had done } immunocytochemistry with standard gold-conjugated secondaries. After } viewing them, I offered him the standard advice that next time we would } mount some sections on nickel or gold grids if he let us know in } advance } that he would be doing immunocytochemistry. He said he always used } copper and asked why he should switch. My answer was something like } "Umm-uhhh.....because it's always done that way" with an additional } mumble about copper interfering with the labeling reaction somehow. } } Now I have another client, also infinitely more savvy in chemistry that } I am, asking the same question. } } So I checked through our little in-house research library (again) and } did some targeted Googling (again) and found that some folks say that } copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use } that anyway); 2) may react with PBS buffers to produce fine } precipitates (but, but....doesn't that mean we should NEVER use Cu with } PBS?); 3) reacts with gold colloid (no other explanation given), 4) } can alter the charge distribution of the section and cause non-specific } background label; and 5) may be oxidized during labelling or } interfere } with oxidation of chemical groups in the tissue to be labelled. } Mostly } people just say to use Ni or Au without stating any reasons. } } Also, a glance through the literature shows that it's not uncommon to } find perfectly successful immunolabelling done on copper grids. } } Now, if I were determined to invade a country called Copper any or all } of the above reasons could be used with little further explanation, but } I'd like to know the Truth and pass it along to my admiring customers. } } Any takers? } } Thanks in advance. } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } } } } } ==============================Original } Headers============================== } 15, 23 -- From TindallR-at-missouri.edu Tue Jul 12 17:12:03 2005 } 15, 23 -- Received: from um-exproto9.um.umsystem.edu } (um-exproto9.um.umsystem.edu [207.160.151.49]) } 15, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6CMC2XX014275 } 15, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 17:12:03 } -0500 } 15, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) } by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 15, 23 -- Tue, 12 Jul 2005 17:11:56 -0500 } 15, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 15, 23 -- Content-class: urn:content-classes:message } 15, 23 -- MIME-Version: 1.0 } 15, 23 -- Content-Type: text/plain; } 15, 23 -- charset="us-ascii" } 15, 23 -- Subject: TEM: Immunocytochemistry and Cu grids } 15, 23 -- Date: Tue, 12 Jul 2005 17:11:47 -0500 } 15, 23 -- Message-ID: } {BA876152E8653240BE8572E897083EE79802F3-at-UM-EMAIL09.um.umsystem.edu} } 15, 23 -- X-MS-Has-Attach: } 15, 23 -- X-MS-TNEF-Correlator: } 15, 23 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids } 15, 23 -- Thread-Index: AcWHLrKQfT52UG1vRkSz6rcfHcA8yA== } 15, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 15, 23 -- To: {microscopy-at-microscopy.com} } 15, 23 -- X-OriginalArrivalTime: 12 Jul 2005 22:11:56.0360 (UTC) } FILETIME=[B74F4C80:01C5872E] } 15, 23 -- Content-Transfer-Encoding: 8bit } 15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j6CMC2XX014275 } ==============================End of - } Headers============================== } }
-- Marc Pypaert Department of Cell Biology Center for Cell and Molecular Imaging Ludwig Institute for Cancer Research Yale University School of Medicine 333 Cedar Street, PO Box 208002 New Haven, CT 06520-8002 TEL 203-785 3681 FAX 203-785 7446
==============================Original Headers============================== 6, 18 -- From marc.pypaert-at-yale.edu Wed Jul 13 10:37:11 2005 6, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DFbBff005748 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 10:37:11 -0500 6, 18 -- Received: from yale.edu (net234-111.med.yale.edu [130.132.234.111]) 6, 18 -- by biomed.med.yale.edu (PMDF V6.1-1 #30532) 6, 18 -- with ESMTP id {01LQKPAV9P3Y007BQ2-at-biomed.med.yale.edu} for 6, 18 -- microscopy-at-microscopy.com; Wed, 13 Jul 2005 11:20:03 -0400 (EDT) 6, 18 -- Date: Wed, 13 Jul 2005 11:19:42 -0400 6, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu} 6, 18 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 6, 18 -- In-reply-to: {200507122213.j6CMDcxr016073-at-ns.microscopy.com} 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- Message-id: {896F9E09-F3B1-11D9-AC3D-0030659833B4-at-yale.edu} 6, 18 -- MIME-version: 1.0 (Apple Message framework v553) 6, 18 -- X-Mailer: Apple Mail (2.553) 6, 18 -- Content-type: text/plain; format=flowed; charset=US-ASCII; delsp=yes 6, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
It doesn't make a lot of difference to me either way, except to be able to actually have an intelligent explanation when our users ask questions about our methods. That said, there are times when we do get severe image distortion in our TEM when using Ni grids with a healthy charge on them. But my main motivation was curiosity, nada mas.
Randy
-----Original Message----- X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu] Sent: Wednesday, July 13, 2005 10:39 AM To: Tindall, Randy D.
What's the big deal about preferring Cu to Ni anyway? Right, there is the charging problem with Nickel, but if you use anti-magnetic tweezers they are just as easy to handle than copper. And the price is really not that much more! We sometimes have to leave grids overnight or longer on solutions, so to be safe we use nickel for this. Since we don't want to make and keep separate stocks of copper and nickel, we switched entirely to nickel grids, and have had no problems with any of our applications. Am I missing something here?!!
Marc
On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote:
} } } } ---------------------------------------------------------------------- } - } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } - } ----- } } Dear Listers, } } I have another one of my "back to the basics" questions that probably } make people wonder how the devil I ever got into this business in the } first place, but here goes anyway. } } We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with
} a bunch of sections on copper mesh grids which he had done } immunocytochemistry with standard gold-conjugated secondaries. After } viewing them, I offered him the standard advice that next time we } would mount some sections on nickel or gold grids if he let us know in
} advance that he would be doing immunocytochemistry. He said he always
} used copper and asked why he should switch. My answer was something } like "Umm-uhhh.....because it's always done that way" with an } additional mumble about copper interfering with the labeling reaction } somehow. } } Now I have another client, also infinitely more savvy in chemistry } that I am, asking the same question. } } So I checked through our little in-house research library (again) and } did some targeted Googling (again) and found that some folks say that } copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use } that anyway); 2) may react with PBS buffers to produce fine } precipitates (but, but....doesn't that mean we should NEVER use Cu with } PBS?); 3) reacts with gold colloid (no other explanation given), 4) } can alter the charge distribution of the section and cause } non-specific background label; and 5) may be oxidized during } labelling or interfere } with oxidation of chemical groups in the tissue to be labelled. } Mostly } people just say to use Ni or Au without stating any reasons. } } Also, a glance through the literature shows that it's not uncommon to } find perfectly successful immunolabelling done on copper grids. } } Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, } but I'd like to know the Truth and pass it along to my admiring customers. } } Any takers? } } Thanks in advance. } } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } } } } } ==============================Original } Headers============================== } 15, 23 -- From TindallR-at-missouri.edu Tue Jul 12 17:12:03 2005 15, 23 } -- Received: from um-exproto9.um.umsystem.edu } (um-exproto9.um.umsystem.edu [207.160.151.49]) } 15, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6CMC2XX014275 } 15, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 17:12:03 } -0500 } 15, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31])
} by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 15, 23 -- Tue, 12 Jul 2005 17:11:56 -0500 } 15, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 15, 23
-- Marc Pypaert Department of Cell Biology Center for Cell and Molecular Imaging Ludwig Institute for Cancer Research Yale University School of Medicine 333 Cedar Street, PO Box 208002 New Haven, CT 06520-8002 TEL 203-785 3681 FAX 203-785 7446
==============================Original Headers============================== 6, 18 -- From marc.pypaert-at-yale.edu Wed Jul 13 10:37:11 2005 6, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DFbBff005748 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 10:37:11 -0500 6, 18 -- Received: from yale.edu (net234-111.med.yale.edu [130.132.234.111]) 6, 18 -- by biomed.med.yale.edu (PMDF V6.1-1 #30532) 6, 18 -- with ESMTP id {01LQKPAV9P3Y007BQ2-at-biomed.med.yale.edu} for 6, 18 -- microscopy-at-microscopy.com; Wed, 13 Jul 2005 11:20:03 -0400 (EDT) 6, 18 -- Date: Wed, 13 Jul 2005 11:19:42 -0400 6, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu} 6, 18 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 6, 18 -- In-reply-to: {200507122213.j6CMDcxr016073-at-ns.microscopy.com} 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- Message-id: {896F9E09-F3B1-11D9-AC3D-0030659833B4-at-yale.edu} 6, 18 -- MIME-version: 1.0 (Apple Message framework v553) 6, 18 -- X-Mailer: Apple Mail (2.553) 6, 18 -- Content-type: text/plain; format=flowed; charset=US-ASCII; delsp=yes 6, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 22, 23 -- From TindallR-at-missouri.edu Wed Jul 13 10:56:21 2005 22, 23 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 22, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DFuLiE013730 22, 23 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 10:56:21 -0500 22, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 22, 23 -- Wed, 13 Jul 2005 10:56:21 -0500 22, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 22, 23 -- Content-class: urn:content-classes:message 22, 23 -- MIME-Version: 1.0 22, 23 -- Content-Type: text/plain; 22, 23 -- charset="us-ascii" 22, 23 -- Subject: RE: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids 22, 23 -- Date: Wed, 13 Jul 2005 10:56:20 -0500 22, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE79802F4-at-UM-EMAIL09.um.umsystem.edu} 22, 23 -- X-MS-Has-Attach: 22, 23 -- X-MS-TNEF-Correlator: 22, 23 -- Thread-Topic: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids 22, 23 -- Thread-Index: AcWHwPNRIL3xOIhMTey96wt+T7YehAAAdtIg 22, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 22, 23 -- To: {microscopy-at-microscopy.com} 22, 23 -- X-OriginalArrivalTime: 13 Jul 2005 15:56:21.0191 (UTC) FILETIME=[69B72570:01C587C3] 22, 23 -- Content-Transfer-Encoding: 8bit 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6DFuLiE013730 ==============================End of - Headers==============================
The main problem encountered with copper grids used for immunocytochemistry or immunogold labeling is the reaction of the copper with salts in the buffers used during labeling. This is a time-related reaction, and can usually be avoided by having short labeling runs and using grids with films on them. Gilder grids, available from major microscope supply vendors, are gold-coated copper grids, and are not that much more expensive than regular copper grids. These grids may be the variety used by researchers who have reported having no problems with their grids during their immuno runs. I usually use nickel grids for my work, but have used formvar-coated copper grids without problems for 1 day immuno runs so long as the film is intact and as long as I don't get clumsy and end up sinking my grids in my solutions (if I do sink them, I do a quick distilled water rinse, blot the back of the grids with filter paper until almost dry, then re-float them where I left off). As others have shared, the nickel grids are much sturdier, not too expensive, and are not a problem to handle as long as you use antimagnetic forceps. With the nickel grids, remember to correct for astigmatism in the TEM by using a hole in your sample on the nickel grid. This will accommodate for any inherent residual magnetic field in the grid itself.
Edward Haller Lab Manager, Diagnostic Electron Microscopy Lab University of South Florida Pathology Department Tampa, FL 33612 (813) 974-9584
==============================Original Headers============================== 4, 26 -- From ehaller-at-hsc.usf.edu Wed Jul 13 11:22:28 2005 4, 26 -- Received: from hscantivirus.hsc.usf.edu (hscantivirus.hsc.usf.edu [131.247.67.157]) 4, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6DGMRdH021865 4, 26 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 11:22:28 -0500 4, 26 -- Received: from HSCMAIL.hscnet.hsc.usf.edu ([IP=131.247.67.144]) by eSafe SMTP Relay 1121174809; Wed Jul 13 12:21:14 2005 4, 26 -- Received: from CONEXCHANGE.hscnet.hsc.usf.edu ([131.247.67.142]) by HSCMAIL.hscnet.hsc.usf.edu with Microsoft SMTPSVC(6.0.3790.0); 4, 26 -- Wed, 13 Jul 2005 12:19:37 -0400 4, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 4, 26 -- Content-class: urn:content-classes:message 4, 26 -- MIME-Version: 1.0 4, 26 -- Content-Type: text/plain; 4, 26 -- charset="US-ASCII" 4, 26 -- Subject: RE: [Microscopy] TEM: Immunocytochemistry and Cu grids 4, 26 -- Date: Wed, 13 Jul 2005 12:19:37 -0400 4, 26 -- Message-ID: {841D767DCDE87C49A02DA6DFC0BABABF691823-at-COMEXCHANGE.hscnet.hsc.usf.edu} 4, 26 -- X-MS-Has-Attach: 4, 26 -- X-MS-TNEF-Correlator: 4, 26 -- Thread-Topic: [Microscopy] TEM: Immunocytochemistry and Cu grids 4, 26 -- Thread-Index: AcWHw4VKXyuYUb6ZSuiO7MbvaO3xNAAALgXg 4, 26 -- From: "Haller, Ed" {ehaller-at-hsc.usf.edu} 4, 26 -- To: {microscopy-at-microscopy.com} 4, 26 -- X-OriginalArrivalTime: 13 Jul 2005 16:19:37.0495 (UTC) FILETIME=[A9FA3A70:01C587C6] 4, 26 -- X-ESAFE-STATUS: Mail clean 4, 26 -- X-ESAFE-DETAILS: Clean 4, 26 -- Content-Transfer-Encoding: 8bit 4, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6DGMRdH021865 ==============================End of - Headers==============================
Sorry for any misunderstanding - My response was not really directed at you, but at the EM community in general who for some reason seems to be biased towards copper. I have never understood this. I have been doing EM for nearly 20 years, exclusively using nickel grids, and would really like to find out if I was mistaken this entire time!! Or is it just another case of "dogma", like Paul put in nicely in his posting? Anyway, I will read with interest the responses you get to your posting! Best
Marc
On Wednesday, July 13, 2005, at 11:57 AM, TindallR-at-missouri.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Marc, } } It doesn't make a lot of difference to me either way, except to be able } to actually have an intelligent explanation when our users ask } questions } about our methods. That said, there are times when we do get severe } image distortion in our TEM when using Ni grids with a healthy charge } on } them. But my main motivation was curiosity, nada mas. } } Randy } } -----Original Message----- } X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu] } Sent: Wednesday, July 13, 2005 10:39 AM } To: Tindall, Randy D. } Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids } } } } } ----------------------------------------------------------------------- } - } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } - } ---- } } What's the big deal about preferring Cu to Ni anyway? } Right, there is the charging problem with Nickel, but if you use } anti-magnetic tweezers they are just as easy to handle than copper. And } the price is really not that much more! We sometimes have to leave } grids } overnight or longer on solutions, so to be safe we use nickel for this. } Since we don't want to make and keep separate stocks of copper and } nickel, we switched entirely to nickel grids, and have had no problems } with any of our applications. Am I missing something here?!! } } Marc } } On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote: } } } } } } } } } ---------------------------------------------------------------------- } } - } } ----- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } - } } ----- } } } } Dear Listers, } } } } I have another one of my "back to the basics" questions that probably } } make people wonder how the devil I ever got into this business in the } } first place, but here goes anyway. } } } } We have a client who prepares his own blocks and brings them to us for } } } sectioning, staining, viewing, etc. One day he came into the lab with } } } a bunch of sections on copper mesh grids which he had done } } immunocytochemistry with standard gold-conjugated secondaries. After } } viewing them, I offered him the standard advice that next time we } } would mount some sections on nickel or gold grids if he let us know in } } } advance that he would be doing immunocytochemistry. He said he always } } } used copper and asked why he should switch. My answer was something } } like "Umm-uhhh.....because it's always done that way" with an } } additional mumble about copper interfering with the labeling reaction } } somehow. } } } } Now I have another client, also infinitely more savvy in chemistry } } that I am, asking the same question. } } } } So I checked through our little in-house research library (again) and } } did some targeted Googling (again) and found that some folks say that } } copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use } } that anyway); 2) may react with PBS buffers to produce fine } } precipitates (but, but....doesn't that mean we should NEVER use Cu } with } } PBS?); 3) reacts with gold colloid (no other explanation given), 4) } } can alter the charge distribution of the section and cause } } non-specific background label; and 5) may be oxidized during } } labelling or interfere } } with oxidation of chemical groups in the tissue to be labelled. } } Mostly } } people just say to use Ni or Au without stating any reasons. } } } } Also, a glance through the literature shows that it's not uncommon to } } find perfectly successful immunolabelling done on copper grids. } } } } Now, if I were determined to invade a country called Copper any or all } } } of the above reasons could be used with little further explanation, } } but I'd like to know the Truth and pass it along to my admiring } customers. } } } } Any takers? } } } } Thanks in advance. } } } } Randy } } } } Randy Tindall } } EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W122 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } } } } } } } } } } } } } } } ==============================Original } } Headers============================== } } 15, 23 -- From TindallR-at-missouri.edu Tue Jul 12 17:12:03 2005 15, 23 } } -- Received: from um-exproto9.um.umsystem.edu } } (um-exproto9.um.umsystem.edu [207.160.151.49]) } } 15, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } j6CMC2XX014275 } } 15, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2005 } 17:12:03 } } -0500 } } 15, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) } } } by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } } 15, 23 -- Tue, 12 Jul 2005 17:11:56 -0500 } } 15, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 15, 23 } } } -- Content-class: urn:content-classes:message 15, 23 -- MIME-Version: } } 1.0 15, 23 -- Content-Type: text/plain; } } 15, 23 -- charset="us-ascii" } } 15, 23 -- Subject: TEM: Immunocytochemistry and Cu grids 15, 23 -- } } Date: Tue, 12 Jul 2005 17:11:47 -0500 15, 23 -- Message-ID: } } {BA876152E8653240BE8572E897083EE79802F3-at-UM-EMAIL09.um.umsystem.edu} } } 15, 23 -- X-MS-Has-Attach: } } 15, 23 -- X-MS-TNEF-Correlator: } } 15, 23 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids 15, 23 } } -- Thread-Index: AcWHLrKQfT52UG1vRkSz6rcfHcA8yA== 15, 23 -- From: } } "Tindall, Randy D." {TindallR-at-missouri.edu} 15, 23 -- To: } } {microscopy-at-microscopy.com} 15, 23 -- X-OriginalArrivalTime: 12 Jul } } 2005 22:11:56.0360 (UTC) FILETIME=[B74F4C80:01C5872E] 15, 23 -- } } Content-Transfer-Encoding: 8bit 15, 23 -- X-MIME-Autoconverted: from } } quoted-printable to 8bit by ns.microscopy.com id j6CMC2XX014275 } } ==============================End of - } } Headers============================== } } } } } } -- } Marc Pypaert } Department of Cell Biology } Center for Cell and Molecular Imaging } Ludwig Institute for Cancer Research } Yale University School of Medicine } 333 Cedar Street, PO Box 208002 } New Haven, CT 06520-8002 } TEL 203-785 3681 } FAX 203-785 7446 } } } ==============================Original } Headers============================== } 6, 18 -- From marc.pypaert-at-yale.edu Wed Jul 13 10:37:11 2005 6, 18 -- } Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu } [130.132.232.48]) } 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6DFbBff005748 } 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 } 10:37:11 -0500 } 6, 18 -- Received: from yale.edu (net234-111.med.yale.edu } [130.132.234.111]) 6, 18 -- by biomed.med.yale.edu (PMDF V6.1-1 } #30532) } 6, 18 -- with ESMTP id {01LQKPAV9P3Y007BQ2-at-biomed.med.yale.edu} for 6, } 18 -- microscopy-at-microscopy.com; Wed, 13 Jul 2005 11:20:03 -0400 (EDT) } 6, 18 -- Date: Wed, 13 Jul 2005 11:19:42 -0400 6, 18 -- From: Marc } Pypaert {marc.pypaert-at-yale.edu} 6, 18 -- Subject: Re: [Microscopy] TEM: } Immunocytochemistry and Cu grids 6, 18 -- In-reply-to: } {200507122213.j6CMDcxr016073-at-ns.microscopy.com} } 6, 18 -- To: microscopy-at-microscopy.com } 6, 18 -- Message-id: {896F9E09-F3B1-11D9-AC3D-0030659833B4-at-yale.edu} } 6, 18 -- MIME-version: 1.0 (Apple Message framework v553) 6, 18 -- } X-Mailer: Apple Mail (2.553) 6, 18 -- Content-type: text/plain; } format=flowed; charset=US-ASCII; delsp=yes 6, 18 -- } Content-transfer-encoding: 7bit ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 22, 23 -- From TindallR-at-missouri.edu Wed Jul 13 10:56:21 2005 } 22, 23 -- Received: from um-exproto8.um.umsystem.edu } (um-exproto8.um.umsystem.edu [207.160.151.48]) } 22, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6DFuLiE013730 } 22, 23 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 10:56:21 } -0500 } 22, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) } by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 22, 23 -- Wed, 13 Jul 2005 10:56:21 -0500 } 22, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 22, 23 -- Content-class: urn:content-classes:message } 22, 23 -- MIME-Version: 1.0 } 22, 23 -- Content-Type: text/plain; } 22, 23 -- charset="us-ascii" } 22, 23 -- Subject: RE: [Microscopy] Re: TEM: Immunocytochemistry and } Cu grids } 22, 23 -- Date: Wed, 13 Jul 2005 10:56:20 -0500 } 22, 23 -- Message-ID: } {BA876152E8653240BE8572E897083EE79802F4-at-UM-EMAIL09.um.umsystem.edu} } 22, 23 -- X-MS-Has-Attach: } 22, 23 -- X-MS-TNEF-Correlator: } 22, 23 -- Thread-Topic: [Microscopy] Re: TEM: Immunocytochemistry and } Cu grids } 22, 23 -- Thread-Index: AcWHwPNRIL3xOIhMTey96wt+T7YehAAAdtIg } 22, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 22, 23 -- To: {microscopy-at-microscopy.com} } 22, 23 -- X-OriginalArrivalTime: 13 Jul 2005 15:56:21.0191 (UTC) } FILETIME=[69B72570:01C587C3] } 22, 23 -- Content-Transfer-Encoding: 8bit } 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j6DFuLiE013730 } ==============================End of - } Headers============================== } }
-- Marc Pypaert Department of Cell Biology Center for Cell and Molecular Imaging Ludwig Institute for Cancer Research Yale University School of Medicine 333 Cedar Street, PO Box 208002 New Haven, CT 06520-8002 TEL 203-785 3681 FAX 203-785 7446
==============================Original Headers============================== 7, 18 -- From marc.pypaert-at-yale.edu Wed Jul 13 12:01:02 2005 7, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48]) 7, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DH11b8030049 7, 18 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 12:01:02 -0500 7, 18 -- Received: from yale.edu (net234-111.med.yale.edu [130.132.234.111]) 7, 18 -- by biomed.med.yale.edu (PMDF V6.1-1 #30532) 7, 18 -- with ESMTP id {01LQKSJRTF2K009625-at-biomed.med.yale.edu} for 7, 18 -- microscopy-at-microscopy.com; Wed, 13 Jul 2005 12:52:45 -0400 (EDT) 7, 18 -- Date: Wed, 13 Jul 2005 12:52:22 -0400 7, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu} 7, 18 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 7, 18 -- In-reply-to: {200507131557.j6DFv7KK015520-at-ns.microscopy.com} 7, 18 -- To: microscopy-at-microscopy.com 7, 18 -- Message-id: {7B66628D-F3BE-11D9-AC3D-0030659833B4-at-yale.edu} 7, 18 -- MIME-version: 1.0 (Apple Message framework v553) 7, 18 -- X-Mailer: Apple Mail (2.553) 7, 18 -- Content-type: text/plain; format=flowed; charset=US-ASCII; delsp=yes 7, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Over the years I have frequently seen cases where the samples themselves have reacted with the copper grid. Two examples:
1) Looking at Fe-S precipitates in magnetotactic bacteria, when the precipitates were ugly and analyzed as copper sulphide. Our surmise was that the iron and copper had undergone ion exchange while the grid (and carbon film) was wet with the culture medium. When we used nickel grids, the precipitates were well formed and composed of iron and sulphur.
2) Looking at Si precipitates in Al-Si electronic bond wire. The specimens were prepared by embedding and microtoming, floating in DI water. The Si precipitates were surrounded by an ugly mess containing masses of copper. Again, presumed to me electrochemical reaction between the Al and the Cu grid, through the DI water medium, somehow preferentially at the Si precipitates. Again, use of Ni grids produced good pictures allowing us to characterize the precipitation.
Having said all that, we continue to default to use copper grids!
Tony.
*********************************************** Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
Thanks for your weigh-in on this subject. I was sorry to learn of your hearing problems; has this been coming on gradually or did something happen (i.e. a severe cold, etc.)to bring the hearing loss on?
I was surprised to learn that Boston is not yet out of the running for an MSA meeting! If I can help in any way, I'll throw my hat in the ring.
Hope your summer (now that it is the middle of July!) is going well.
Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
-----Original Message----- X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU] Sent: Wednesday, July 13, 2005 1:33 PM To: Sherwood, Margaret
Over the years I have frequently seen cases where the samples themselves have reacted with the copper grid. Two examples:
1) Looking at Fe-S precipitates in magnetotactic bacteria, when the precipitates were ugly and analyzed as copper sulphide. Our surmise was that the iron and copper had undergone ion exchange while the grid (and carbon film) was wet with the culture medium. When we used nickel grids, the precipitates were well formed and composed of iron and sulphur.
2) Looking at Si precipitates in Al-Si electronic bond wire. The specimens were prepared by embedding and microtoming, floating in DI water. The Si precipitates were surrounded by an ugly mess containing masses of copper. Again, presumed to me electrochemical reaction between the Al and the Cu grid, through the DI water medium, somehow preferentially at the Si precipitates. Again, use of Ni grids produced good pictures allowing us to characterize the precipitation.
Having said all that, we continue to default to use copper grids!
Tony.
*********************************************** Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tpepper-at-iastate.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, July 13, 2005 at 09:21:59 ---------------------------------------------------------------------------
Email: tpepper-at-iastate.edu Name: Tracey Pepper
Organization: Iowa State University
Title-Subject: [Filtered] MListserver:
Question: HELP! We are in desparate need of some assisitance! We have a scientist in Texas who is in the field collecting crucial samples for TEM examination. They must be collected in the next couple of days. Our efforts to send (via DHL) good fixative (standard em fix for plants a double aldehyde in a 0.1 M cacodylate) have failed! The person is located in Plainview, Texas. Is there anyone on this listserver who could make us some fixtive (150 mls)for this person to come pick up? Preferably within an hour radius? If you can, Please call 515-294-3872 as soon as possible. We would be in your debt!! With Great appreciation, Tracey Pepper Bessey Microscopy Facility Iowa State University Ames, IA 50011-1020
Your best bet would be to call some one at Texas Tech in Lubbock http://www.macmed.ttuhsc.edu/ They may be more than an hour from Plainview but that close to the edge of the world. Your only other chance is Amarillo with the TAM Vet Med Diagnostic lab http://www.medcenter.org/facilities/am-vetlab.html.
Good luck Gordon
Gordon Couger I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
tpepper-at-iastate.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by } (tpepper-at-iastate.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } on Wednesday, July 13, 2005 at 09:21:59 } --------------------------------------------------------------------------- } } Email: tpepper-at-iastate.edu } Name: Tracey Pepper } } Organization: Iowa State University } } Title-Subject: [Filtered] MListserver: } } Question: HELP! We are in desparate need of some assisitance! We have a scientist in Texas who is in the field collecting crucial samples for TEM examination. They must be collected in the next couple of days. Our efforts to send (via DHL) good fixative (standard em fix for plants a double aldehyde in a 0.1 M cacodylate) have failed! The person is located in Plainview, Texas. Is there anyone on this listserver who could make us some fixtive (150 mls)for this person to come pick up? Preferably within an hour radius? If you can, Please call 515-294-3872 as soon as possible. We would be in your debt!! } With Great appreciation, } Tracey Pepper } Bessey Microscopy Facility } Iowa State University } Ames, IA 50011-1020 } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Wed Jul 13 13:54:01 2005 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DIs1ei022223 } 6, 12 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 13:54:01 -0500 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110400befb1371ac5b-at-[206.69.208.22]} } 6, 12 -- Date: Wed, 13 Jul 2005 13:54:00 -0500 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: tpepper-at-iastate.edu (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: Help with Fixative near Plainview Texas } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } }
==============================Original Headers============================== 5, 20 -- From gcc-at-couger.com Wed Jul 13 15:01:27 2005 5, 20 -- Received: from lakermmtao08.cox.net (lakermmtao08.cox.net [68.230.240.31]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DK1RJr030657 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 15:01:27 -0500 5, 20 -- Received: from [127.0.0.1] (really [68.12.43.168]) by lakermmtao08.cox.net 5, 20 -- (InterMail vM.6.01.04.00 201-2131-118-20041027) with ESMTP 5, 20 -- id {20050713200117.YBBU19415.lakermmtao08.cox.net-at-[127.0.0.1]} 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 16:01:17 -0400 5, 20 -- Message-ID: {42D5730D.4090609-at-couger.com} 5, 20 -- Date: Wed, 13 Jul 2005 15:01:17 -0500 5, 20 -- From: Gordon Couger {gcc-at-couger.com} 5, 20 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 5, 20 -- X-Accept-Language: en-us, en 5, 20 -- MIME-Version: 1.0 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Subject: Re: [Microscopy] viaWWW: Help with Fixative near Plainview Texas 5, 20 -- References: {200507131858.j6DIw5Mo027248-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200507131858.j6DIw5Mo027248-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does anyone have a Nikon Diaphot epi-fluorescence lamp house and filter set/holder for sale? Alternatively, if anyone knows of a second hand microscope parts vendor?
Thanks for your help.
Regards
Andrew
________________________________________________________________________ ______ Andrew McNaughton Otago Centre for Confocal Microscopy C/-Department of Anatomy and Structural Biology School of Medical Sciences University of Otago PO Box 913, Dunedin NEW ZEALAND
I am using titanium tweezers with nickel grids - they are cheap nowadays and I like them (tweezers) for light weight and soft action. I am using carbon coating on top of all my plastic films (copper or nickel grids) and have no problems with charge/astigmatism. I suspect, charging problem happens when grids quite old and/or dirty. Sergey
At 09:23 AM 7/13/2005, you wrote:
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==============================Original Headers============================== 6, 25 -- From sryazant-at-ucla.edu Wed Jul 13 16:09:43 2005 6, 25 -- Received: from smtp-3.smtp.ucla.edu (smtp-3.smtp.ucla.edu [169.232.48.136]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DL9gde014359 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 16:09:43 -0500 6, 25 -- Received: from mail.ucla.edu (mail.ucla.edu [169.232.48.141]) 6, 25 -- by smtp-3.smtp.ucla.edu (8.13.4/8.13.4) with ESMTP id j6DL9gFM012306 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 14:09:42 -0700 6, 25 -- Received: from kopoba.ucla.edu (ts13-161.dialup.bol.ucla.edu [169.232.229.108]) 6, 25 -- (authenticated bits=0) 6, 25 -- by mail.ucla.edu (8.13.4/8.13.4) with ESMTP id j6DL9YYN006121 6, 25 -- (version=TLSv1/SSLv3 cipher=DES-CBC3-SHA bits=168 verify=NOT) 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 14:09:38 -0700 6, 25 -- Message-Id: {6.1.2.0.2.20050713140435.032c38b0-at-mail.ucla.edu} 6, 25 -- X-Sender: sryazant-at-mail.ucla.edu 6, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 25 -- Date: Wed, 13 Jul 2005 14:09:25 -0700 6, 25 -- To: microscopy-at-microscopy.com 6, 25 -- From: Sergey {sryazant-at-ucla.edu} 6, 25 -- Subject: Re: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids 6, 25 -- In-Reply-To: {200507131623.j6DGNPQ6024061-at-ns.microscopy.com} 6, 25 -- References: {200507131623.j6DGNPQ6024061-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 25 -- X-Probable-Spam: no 6, 25 -- X-Scanned-By: smtp.ucla.edu on 169.232.48.136 ==============================End of - Headers==============================
John Oren, Vermontoptech 802 425 2040 ----- Original Message ----- X-from: {andrew.mcnaughton-at-stonebow.otago.ac.nz} To: {micro-at-superlink.net} Sent: Wednesday, July 13, 2005 4:43 PM
Actually, TTU is only about 45 minutes from Plainview, and both the Dept. of Bio. Sci. and the TTU Medical School have electron microscopy labs.
-----Original Message----- X-from: gcc-at-couger.com [mailto:gcc-at-couger.com] Sent: Wednesday, July 13, 2005 1:05 PM To: jfb-at-uidaho.edu
Your best bet would be to call some one at Texas Tech in Lubbock http://www.macmed.ttuhsc.edu/ They may be more than an hour from Plainview but that close to the edge of the world. Your only other chance is Amarillo with the TAM Vet Med Diagnostic lab http://www.medcenter.org/facilities/am-vetlab.html.
Good luck Gordon
Gordon Couger I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
tpepper-at-iastate.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by } (tpepper-at-iastate.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } on Wednesday, July 13, 2005 at 09:21:59 } --------------------------------------------------------------------------- } } Email: tpepper-at-iastate.edu } Name: Tracey Pepper } } Organization: Iowa State University } } Title-Subject: [Filtered] MListserver: } } Question: HELP! We are in desparate need of some assisitance! We have a scientist in Texas who is in the field collecting crucial samples for TEM examination. They must be collected in the next couple of days. Our efforts to send (via DHL) good fixative (standard em fix for plants a double aldehyde in a 0.1 M cacodylate) have failed! The person is located in Plainview, Texas. Is there anyone on this listserver who could make us some fixtive (150 mls)for this person to come pick up? Preferably within an hour radius? If you can, Please call 515-294-3872 as soon as possible. We would be in your debt!! } With Great appreciation, } Tracey Pepper } Bessey Microscopy Facility } Iowa State University } Ames, IA 50011-1020 } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Wed Jul 13 13:54:01 2005 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DIs1ei022223 } 6, 12 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 13:54:01 -0500 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110400befb1371ac5b-at-[206.69.208.22]} } 6, 12 -- Date: Wed, 13 Jul 2005 13:54:00 -0500 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: tpepper-at-iastate.edu (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: Help with Fixative near Plainview Texas } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } }
==============================Original Headers============================== 5, 20 -- From gcc-at-couger.com Wed Jul 13 15:01:27 2005 5, 20 -- Received: from lakermmtao08.cox.net (lakermmtao08.cox.net [68.230.240.31]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DK1RJr030657 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 15:01:27 -0500 5, 20 -- Received: from [127.0.0.1] (really [68.12.43.168]) by lakermmtao08.cox.net 5, 20 -- (InterMail vM.6.01.04.00 201-2131-118-20041027) with ESMTP 5, 20 -- id {20050713200117.YBBU19415.lakermmtao08.cox.net-at-[127.0.0.1]} 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 16:01:17 -0400 5, 20 -- Message-ID: {42D5730D.4090609-at-couger.com} 5, 20 -- Date: Wed, 13 Jul 2005 15:01:17 -0500 5, 20 -- From: Gordon Couger {gcc-at-couger.com} 5, 20 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 5, 20 -- X-Accept-Language: en-us, en 5, 20 -- MIME-Version: 1.0 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Subject: Re: [Microscopy] viaWWW: Help with Fixative near Plainview Texas 5, 20 -- References: {200507131858.j6DIw5Mo027248-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200507131858.j6DIw5Mo027248-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 14, 27 -- From jfb-at-uidaho.edu Wed Jul 13 16:27:33 2005 14, 27 -- Received: from proofagent.csrv.uidaho.edu (mx2.uidaho.edu [129.101.155.249]) 14, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DLRWRx029989 14, 27 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 16:27:32 -0500 14, 27 -- Received: from mailB.its.uidaho.edu (mailB.its.uidaho.edu [129.101.155.251]) 14, 27 -- by proofagent.csrv.uidaho.edu (8.13.3/8.13.3) with ESMTP id j6DLRVQW020697 14, 27 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 14:27:31 -0700 14, 27 -- Received: from jfb1 (PC006696.fs.uidaho.edu [129.101.141.111]) 14, 27 -- by mailB.its.uidaho.edu (Go Vandals!) 14, 27 -- with SMTP id {0IJL00CI44XVC6-at-mailB.its.uidaho.edu} for 14, 27 -- microscopy-at-microscopy.com; Wed, 13 Jul 2005 14:27:31 -0700 (PDT) 14, 27 -- Date: Wed, 13 Jul 2005 14:27:49 -0700 14, 27 -- From: Franklin Bailey {jfb-at-uidaho.edu} 14, 27 -- Subject: RE: [Microscopy] Re: viaWWW: Help with Fixative near Plainview Texas 14, 27 -- In-reply-to: {200507132004.j6DK4osn002913-at-ns.microscopy.com} 14, 27 -- To: microscopy-at-microscopy.com 14, 27 -- Message-id: {GPEIIPGKOJCCMLKMDOOFAEJDCLAA.jfb-at-uidaho.edu} 14, 27 -- MIME-version: 1.0 14, 27 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 14, 27 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 14, 27 -- Content-type: text/plain; charset=iso-8859-1 14, 27 -- Content-transfer-encoding: 7bit 14, 27 -- Importance: Normal 14, 27 -- X-Priority: 3 (Normal) 14, 27 -- X-MSMail-priority: Normal 14, 27 -- X-SpamDetails: rule=notspam policy=default score=0 mlx=0 adultscore=0 adjust=0 reason=mlx engine=3.0.0-05071100 definitions=3.0.0-05071302 14, 27 -- X-SpamScore: 0 ==============================End of - Headers==============================
Henk: I believe you are correct. The "hi-mag" mode does use an in-lens detector and you will get x-ray peaks you are not wanting due to the way the magnetic lens acts.
Larry: using "Analysis" mode will correct this problem. The WD for this scope with an INCA detector should be about 12mm +/- 1mm. Using the "hi-mag" mode for EDX work is not recommended; you won't get good resolution at this working distance anyway and the in-lens detector won't give a very good image at this distance, besides being very sensitive to charging.
colijn.1-at-osu.edu wrote:
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-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 6, 23 -- From r-holdford-at-ti.com Wed Jul 13 17:39:30 2005 6, 23 -- Received: from go4.ext.ti.com (go4.ext.ti.com [192.91.75.132]) 6, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6DMdTS1005980 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 17:39:30 -0500 6, 23 -- Received: from dlep30.itg.ti.com ([157.170.139.157]) 6, 23 -- by go4.ext.ti.com (8.13.1/8.13.1) with ESMTP id j6DMdN08019399 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 17:39:28 -0500 (CDT) 6, 23 -- Received: from [156.117.194.45] (localhost [127.0.0.1]) 6, 23 -- by dlep30.itg.ti.com (8.12.11/8.12.11) with ESMTP id j6DMdN4b018550 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2005 17:39:23 -0500 (CDT) 6, 23 -- Message-ID: {42D5981A.8050700-at-ti.com} 6, 23 -- Date: Wed, 13 Jul 2005 17:39:22 -0500 6, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 6, 23 -- Organization: SC Packaging Development -- FA Development 6, 23 -- User-Agent: Mozilla Thunderbird 0.8 (Windows/20040913) 6, 23 -- X-Accept-Language: en-us, en 6, 23 -- MIME-Version: 1.0 6, 23 -- To: microscopy-at-microscopy.com 6, 23 -- Subject: Re: [Microscopy] Re: Stray X-rays in FE SEM? 6, 23 -- References: {200507130012.j6D0CZYa029297-at-ns.microscopy.com} 6, 23 -- In-Reply-To: {200507130012.j6D0CZYa029297-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The actual retail price of T221 in the summer of 2004 (when T221 was available from regular retail sources) was only $4K. Proper video adapters are $800 to $1,200, largely matter of taste. It is safe to say the whole deal was approximately. $5K. Totally worth it IMO. The figures around $8K to $10K discussed in this thread are MSRP, not retail prices.
Vitaly Feingold SIA 2773 Heath Lane Duluth, GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {Mike.Bode-at-soft-imaging.net} To: {vitalylazar-at-att.net} Sent: Friday, July 08, 2005 11:01 AM
Hi John,
The advantages besides the obvious superb display are:
1) the ability to display entire image at 100% zoom (pixel-to-pixel sensor-to-monitor) without zoom or pan;
2) have such image and multiple toolbars and control boxes displayed on the desktop without interfering with each other, plus an extra application or two open at the same time, again, not hiding behind other open windows- makes it much easier on the operator.
Works very well for 4 or 6 megapixels camera. Now, for larger sensor such as 11 or 16 megapixels, one would have to zoom out to 50% in order to see entire image at once. Still much better than zooming down to 25% on a regular monitor.
Vitaly Feingold SIA 2773 Heath Lane Duluth, GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {john.mardinly-at-intel.com} To: {vitalylazar-at-att.net} Sent: Friday, July 08, 2005 4:16 PM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (xuy-at-nih.gov) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, July 14, 2005 at 08:09:33 ---------------------------------------------------------------------------
Email: xuy-at-nih.gov Name: Yuhui Xu
Organization: Harvard Medical School
Title-Subject: [Filtered] MListserver:
Question: I am in the process of buying a digital camera for my light microscope ( Leica DM LB2), and would like to get your opinion as to which maker or model is a good choice in terms of resolution, stability, and ease of use, and of course the price.
Once again the MSA Education Committee has organized Exhibitor Demonstrations and Tutorials during the Microscopy and Microanalysis 2005 meeting. This will occur on Tuesday, August 2 starting at 5:00 pm in the Exhibit Hall. These mini-seminars and/or tutorial demonstrations are held in the booths of the participating companies after the Hall is closed to non-participants.
Reservations sheets with titles and descriptions will be at the MSA Education table in the MSA Mega Booth. When you sign up you will be issued a ticket, which you will need to renter the Hall after it is closed. You need to sign up no later than noon on Tuesday. The number of attendees is limited, so visit the MSA Education table soon, since the demonstrations get filled up quickly!
Here's a list of participating Exhibitors and titles:
Your problem is simply one of backscatter bouncing off the final lens irradiating areas off axis, generating the x-rays as appropriate. This is not just a problem with the SEM you mention, it is a general problem of which many people are unaware. The worst case I have ever seen was obtaining x-ray information from 0.75cm (~1/4inch) from the point of initial beam impact!
Modern detectors and collimators do help but scatter is always a problem. I believe that is why SEM manufacturer's main holder is often one which places the specimen surface at a higher level well away from the holder/stage interface. A specimen "in space" will always offer a cleaner x-ray signal.
It is a personal suggestion to clients that when trying hard with an analysis they DO NOT use a multi specimen holder!
Those who wish to know more should read texts relating to the production of SE in relation to those produced by "bounce off" backscatter. We have a short piece in the "Hints and Tips" section of our web site.
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7802 966067 Web www.emcourses.com
----- Original Message ----- X-from: {hanke-at-mee-inc.com} To: {protrain-at-emcourses.com} Sent: Wednesday, July 13, 2005 12:03 AM
We have an old Wild MTr19 illuminator for an equally old Wild model 20 compound light microscope. The bulb in the illuminator burnt out and from the sources I checked that type of bulb is supposedly no longer made. Also, there are no markings on the bulb to indicate what it is. Is anyone familiar with this old illuminator and either knows of bulb replacements or a compatible illuminator for which I can still get bulbs?
Thanks
Norm Olson ______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 4107 Natural Science Building Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu (858)534-5852 Office; (858)534-5846 - Fax ______________________________________________________________
==============================Original Headers============================== 9, 24 -- From nholson-at-ucsd.edu Thu Jul 14 13:52:19 2005 9, 24 -- Received: from mailbox4.ucsd.edu (mailbox4.ucsd.edu [132.239.1.56]) 9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6EIqJbE032597 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 13:52:19 -0500 9, 24 -- Received: from smtp.ucsd.edu (smtp-a.ucsd.edu [132.239.1.49]) 9, 24 -- by mailbox4.ucsd.edu (8.13.3/8.13.3) with ESMTP id j6EIOBhA036898 9, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 11:24:12 -0700 (PDT) 9, 24 -- Received: from [132.239.184.37] ([132.239.184.37]) 9, 24 -- by smtp.ucsd.edu (8.12.10/8.9.3) with ESMTP id j6EIO3Tg020814 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 11:24:11 -0700 (PDT) 9, 24 -- User-Agent: Microsoft-Entourage/10.0.0.1309 9, 24 -- Date: Thu, 14 Jul 2005 11:24:03 -0700 9, 24 -- Subject: Illuminator for old Wild light microscope 9, 24 -- From: Norman Olson {nholson-at-ucsd.edu} 9, 24 -- To: {Microscopy-at-microscopy.com} 9, 24 -- Message-ID: {BEFBFBD3.28C6%nholson-at-ucsd.edu} 9, 24 -- Mime-version: 1.0 9, 24 -- Content-type: text/plain; charset="ISO-8859-1" 9, 24 -- X-Greylisting: NO DELAY (Trusted relay host); 9, 24 -- processed by UCSD_GL-v1.3 on mailbox4.ucsd.edu; 9, 24 -- Thu, 14 July 2005 11:24:12 -0700 (PDT) 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6EIqJbE032597 ==============================End of - Headers==============================
Joe Mears knows everything you what to know about the Swiss made Wild. Microscope Services 1403 Bradley Avenue, Rockville, Maryland 20851 Office 301.294.7960 Fax 301.294.1934 Cell 240.994.7191
Joe also works on Leica, Zeiss, and Nikon light scopes. He is a very good repair person.
nholson-at-ucsd.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have an old Wild MTr19 illuminator for an equally old Wild model 20 } compound light microscope. The bulb in the illuminator burnt out and from } the sources I checked that type of bulb is supposedly no longer made. Also, } there are no markings on the bulb to indicate what it is. Is anyone } familiar with this old illuminator and either knows of bulb replacements or } a compatible illuminator for which I can still get bulbs? } } Thanks } } Norm Olson } ______________________________________________________________ } Norm Olson } Cryoelectron Microscopy Facilities Manager } 4107 Natural Science Building } Department of Chemistry & Biochemistry, MC-0378 } University of California San Diego } La Jolla, CA 92093-0378 } nholson-at-ucsd.edu } http://cryoem.ucsd.edu } (858)534-5852 Office; (858)534-5846 - Fax } ______________________________________________________________ } } } } } } } } ==============================Original Headers============================== } 9, 24 -- From nholson-at-ucsd.edu Thu Jul 14 13:52:19 2005 } 9, 24 -- Received: from mailbox4.ucsd.edu (mailbox4.ucsd.edu [132.239.1.56]) } 9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6EIqJbE032597 } 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 13:52:19 -0500 } 9, 24 -- Received: from smtp.ucsd.edu (smtp-a.ucsd.edu [132.239.1.49]) } 9, 24 -- by mailbox4.ucsd.edu (8.13.3/8.13.3) with ESMTP id j6EIOBhA036898 } 9, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) } 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 11:24:12 -0700 (PDT) } 9, 24 -- Received: from [132.239.184.37] ([132.239.184.37]) } 9, 24 -- by smtp.ucsd.edu (8.12.10/8.9.3) with ESMTP id j6EIO3Tg020814 } 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 11:24:11 -0700 (PDT) } 9, 24 -- User-Agent: Microsoft-Entourage/10.0.0.1309 } 9, 24 -- Date: Thu, 14 Jul 2005 11:24:03 -0700 } 9, 24 -- Subject: Illuminator for old Wild light microscope } 9, 24 -- From: Norman Olson {nholson-at-ucsd.edu} } 9, 24 -- To: {Microscopy-at-microscopy.com} } 9, 24 -- Message-ID: {BEFBFBD3.28C6%nholson-at-ucsd.edu} } 9, 24 -- Mime-version: 1.0 } 9, 24 -- Content-type: text/plain; charset="ISO-8859-1" } 9, 24 -- X-Greylisting: NO DELAY (Trusted relay host); } 9, 24 -- processed by UCSD_GL-v1.3 on mailbox4.ucsd.edu; } 9, 24 -- Thu, 14 July 2005 11:24:12 -0700 (PDT) } 9, 24 -- Content-Transfer-Encoding: 8bit } 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6EIqJbE032597 } ==============================End of - Headers============================== }
-- Chere Petty, M.S. Manager, Keith R. Porter Imaging Facility Department of Biological Sciences University of Maryland Baltimore County (UMBC) 1000 Hilltop Circle Baltimore, MD 21250 Phone: 410-455-2296 Fax: 410-455-3875
"The statements and opinions expressed here by Gary M. Brown represent neither those of ExxonMobil Corporation nor its affiliates."
Norm,
Leica should have a part number for the bulb and the vital stats needed to purchase it elsewhere.
Regards,
Gary M. Brown ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
nholson-at-ucsd.e du To gary.m.brown-at-exxonmobil.com 07/14/05 01:54 cc PM Subject [Microscopy] Illuminator for old Please respond Wild light microscope to microscopy-at-mic roscopy.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
We have an old Wild MTr19 illuminator for an equally old Wild model 20 compound light microscope. The bulb in the illuminator burnt out and from the sources I checked that type of bulb is supposedly no longer made. Also, there are no markings on the bulb to indicate what it is. Is anyone familiar with this old illuminator and either knows of bulb replacements or a compatible illuminator for which I can still get bulbs?
Thanks
Norm Olson ______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 4107 Natural Science Building Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu (858)534-5852 Office; (858)534-5846 - Fax ______________________________________________________________
==============================Original Headers============================== 9, 24 -- From nholson-at-ucsd.edu Thu Jul 14 13:52:19 2005 9, 24 -- Received: from mailbox4.ucsd.edu (mailbox4.ucsd.edu [132.239.1.56]) 9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6EIqJbE032597 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 13:52:19 -0500 9, 24 -- Received: from smtp.ucsd.edu (smtp-a.ucsd.edu [132.239.1.49]) 9, 24 -- by mailbox4.ucsd.edu (8.13.3/8.13.3) with ESMTP id j6EIOBhA036898 9, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 11:24:12 -0700 (PDT) 9, 24 -- Received: from [132.239.184.37] ([132.239.184.37]) 9, 24 -- by smtp.ucsd.edu (8.12.10/8.9.3) with ESMTP id j6EIO3Tg020814 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 11:24:11 -0700 (PDT) 9, 24 -- User-Agent: Microsoft-Entourage/10.0.0.1309 9, 24 -- Date: Thu, 14 Jul 2005 11:24:03 -0700 9, 24 -- Subject: Illuminator for old Wild light microscope 9, 24 -- From: Norman Olson {nholson-at-ucsd.edu} 9, 24 -- To: {Microscopy-at-microscopy.com} 9, 24 -- Message-ID: {BEFBFBD3.28C6%nholson-at-ucsd.edu} 9, 24 -- Mime-version: 1.0 9, 24 -- Content-type: text/plain; charset="ISO-8859-1" 9, 24 -- X-Greylisting: NO DELAY (Trusted relay host); 9, 24 -- processed by UCSD_GL-v1.3 on mailbox4.ucsd.edu; 9, 24 -- Thu, 14 July 2005 11:24:12 -0700 (PDT) 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6EIqJbE032597 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 21 -- From gary.m.brown-at-exxonmobil.com Thu Jul 14 16:37:49 2005 30, 21 -- Received: from hoespc02.exxonmobil.com (hoespc02.exxonmobil.com [192.67.48.39]) 30, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6ELbnYQ017505 30, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 16:37:49 -0500 30, 21 -- Received: from hounmg03.NA.XOM.COM (hounmg03.na.xom.com [158.35.101.41]) 30, 21 -- by hoespc02.exxonmobil.com (Switch-3.1.7/Switch-3.1.7) with ESMTP id j6ELbkCx010176 30, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2005 16:37:49 -0500 (CDT) 30, 21 -- In-Reply-To: {200507141854.j6EIsqtp004351-at-ns.microscopy.com} 30, 21 -- Subject: Re: [Microscopy] Illuminator for old Wild light microscope 30, 21 -- Importance: 30, 21 -- To: microscopy-at-microscopy.com 30, 21 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 30, 21 -- Message-ID: {OF0700E947.8B6F5623-ON8625703E.007675EB-8625703E.0076D0A3-at-exxonmobil.com} 30, 21 -- From: gary.m.brown-at-exxonmobil.com 30, 21 -- Date: Thu, 14 Jul 2005 16:37:46 -0500 30, 21 -- X-MIMETrack: Serialize by Router on Hounmg03.na.xom.com/S/ExxonMobil(652HF702|December 30, 21 -- 14, 2004) at 07/14/2005 04:37:49 PM 30, 21 -- MIME-Version: 1.0 30, 21 -- Content-type: text/plain; charset=ISO-8859-1 30, 21 -- Content-Transfer-Encoding: 8bit 30, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6ELbnYQ017505 ==============================End of - Headers==============================
Does anyone know of a company that can service an old Gatan Duomill? Ours has developed an electrical problem that keeps the guns from energizing.
Thanks in advance.
----------------------------------------- John Bonevich, Ph.D. National Institute of Standards and Technology Metallurgy Division, Mail-Stop 8555 100 Bureau Drive Gaithersburg, MD 20899 USA
==============================Original Headers============================== 5, 20 -- From john.bonevich-at-nist.gov Fri Jul 15 12:11:10 2005 5, 20 -- Received: from smtp.nist.gov (rimp2.nist.gov [129.6.16.227]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6FHBADR005599 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 15 Jul 2005 12:11:10 -0500 5, 20 -- Received: from postmark.nist.gov (pullyou.nist.gov [129.6.16.93]) 5, 20 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id j6FHB7S3009790 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 15 Jul 2005 13:11:07 -0400 5, 20 -- Received: from h180168.nist.gov (h180168.nist.gov [129.6.180.168]) 5, 20 -- by postmark.nist.gov (8.12.5/8.12.5) with ESMTP id j6FHAO6v002771 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 15 Jul 2005 13:10:24 -0400 (EDT) 5, 20 -- Message-Id: {6.2.1.2.2.20050715130621.0389b1e0-at-email.nist.gov} 5, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 5, 20 -- Date: Fri, 15 Jul 2005 13:10:21 -0400 5, 20 -- To: Microscopy-at-MSA.Microscopy.Com 5, 20 -- From: John Bonevich {john.bonevich-at-nist.gov} 5, 20 -- Subject: Gatan Duomill service 5, 20 -- Mime-Version: 1.0 5, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 20 -- X-NIST-MailScanner: Found to be clean 5, 20 -- X-NIST-MailScanner-From: john.bonevich-at-nist.gov ==============================End of - Headers==============================
-----Original Message----- X-from: john.bonevich-at-nist.gov [mailto:john.bonevich-at-nist.gov] Sent: Friday, July 15, 2005 10:15 AM To: curulli-at-usc.edu
Hello,
Does anyone know of a company that can service an old Gatan Duomill? Ours has developed an electrical problem that keeps the guns from energizing.
Thanks in advance.
----------------------------------------- John Bonevich, Ph.D. National Institute of Standards and Technology Metallurgy Division, Mail-Stop 8555 100 Bureau Drive Gaithersburg, MD 20899 USA
==============================Original Headers============================== 5, 20 -- From john.bonevich-at-nist.gov Fri Jul 15 12:11:10 2005 5, 20 -- Received: from smtp.nist.gov (rimp2.nist.gov [129.6.16.227]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6FHBADR005599 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 15 Jul 2005 12:11:10 -0500 5, 20 -- Received: from postmark.nist.gov (pullyou.nist.gov [129.6.16.93]) 5, 20 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id j6FHB7S3009790 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 15 Jul 2005 13:11:07 -0400 5, 20 -- Received: from h180168.nist.gov (h180168.nist.gov [129.6.180.168]) 5, 20 -- by postmark.nist.gov (8.12.5/8.12.5) with ESMTP id j6FHAO6v002771 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 15 Jul 2005 13:10:24 -0400 (EDT) 5, 20 -- Message-Id: {6.2.1.2.2.20050715130621.0389b1e0-at-email.nist.gov} 5, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 5, 20 -- Date: Fri, 15 Jul 2005 13:10:21 -0400 5, 20 -- To: Microscopy-at-MSA.Microscopy.Com 5, 20 -- From: John Bonevich {john.bonevich-at-nist.gov} 5, 20 -- Subject: Gatan Duomill service 5, 20 -- Mime-Version: 1.0 5, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 20 -- X-NIST-MailScanner: Found to be clean 5, 20 -- X-NIST-MailScanner-From: john.bonevich-at-nist.gov ==============================End of - Headers==============================
==============================Original Headers============================== 14, 20 -- From curulli-at-usc.edu Fri Jul 15 12:43:29 2005 14, 20 -- Received: from msg-mx5.usc.edu (msg-mx5.usc.edu [128.125.137.10]) 14, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6FHhSes013552 14, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jul 2005 12:43:28 -0500 14, 20 -- Received: from CEMMA2 ([128.125.153.147]) 14, 20 -- by msg-mx5.usc.edu (Sun Java System Messaging Server 6.2-2.07 (built May 25 14, 20 -- 2005)) with ESMTP id {0IJO0055WJWDOOA0-at-msg-mx5.usc.edu} for 14, 20 -- microscopy-at-microscopy.com; Fri, 15 Jul 2005 10:43:28 -0700 (PDT) 14, 20 -- Date: Fri, 15 Jul 2005 10:43:24 -0700 14, 20 -- From: John Curulli {curulli-at-usc.edu} 14, 20 -- Subject: RE: [Microscopy] Gatan Duomill service 14, 20 -- In-reply-to: {200507151714.j6FHEWHj009497-at-ns.microscopy.com} 14, 20 -- To: microscopy-at-microscopy.com 14, 20 -- Message-id: {0IJO0055ZJWGOOA0-at-msg-mx5.usc.edu} 14, 20 -- MIME-version: 1.0 14, 20 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 14, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 14, 20 -- Content-type: text/plain; charset=us-ascii 14, 20 -- Content-transfer-encoding: 7BIT 14, 20 -- Thread-index: AcWJYKzdElCIuMriSBCO+fa/tcHJtQAA9csA ==============================End of - Headers==============================
I am an amateur and have been experimenting for quite some time with a technique for sharpening silicon carbide (SiC) crystals as an alternative to the very expensive diamond knives commonly used with ultramicrotomes.
The major difficulty is that SiC crystals are very brittle; thus SiC crystals need to be lapped and polished by unconventional methods to prevent micro-mechanical stresses from breaking the edge. Conventional gem faceting technology does not work.
Vitreous carbon knives have also been reported in the literature but did not live up to expectations apparently.
As many on this list will know, glass knives are preferred for their initial sharpness. Meanwhile diamond knives are preferred for their unexcelled durability, flat faces and straight edges, which makes it possible to make almost unlimited numbers of serial sections up to a few millimeters wide.
My efforts indicate that it is possible to make SiC knives and that they can generate silvery sections down to 100 nanometers or so, which puts them in the ball park for practical EM work. My sections do transmit electrons, and at best do not appear to be much worse than diamond knives in regards to their initial sharpness.
Initially I had imagined that SiC knives might potentially replace diamond knives, since the hardness of SiC crystals is 9+ on the Mho's scale, while diamond is 10.
As it turns out, my SiC knives gradually become dull over the course of making hundreds of sections. However they are distinctly more durable than glass knives, and the lapped faces are much flatter and the edges wider compared to glass.
The SiC knife durability seems to be a function of the hardness of the embedding plastic being sectioned, as one may imagine. (They section glycol methacrylate nicely, for making floating ribbons, which is helpful since I intend to use the SiC knives myself for LM work).
My question is how useful such knives are likely to be in practice? I imagine SiC knives might fill a niche market for disposable substitutes for diamond knives, when an inexpensive alternative to glass knives is needed only occasionally and the purchase of a diamond knife doesn't make sense, or maybe for student teaching.
It seems likely that the same technology can probably be used to make sapphire knives of equivalent initial sharpness (sapphire is much tougher and more fracture resistant than SiC but not so hard as SiC). But I don't know, my efforts so far have only concerned SiC knives.
I expect to publish my SiC knife making technology and put it in the public domain, but for now I am soliciting comments on their potential usefulness. -- Roger, Austin, Tx
==============================Original Headers============================== 17, 19 -- From rcbaker-at-eden.infohwy.com Sat Jul 16 10:57:37 2005 17, 19 -- Received: from mx2.lsn.net (mx2.lsn.net [66.90.130.74]) 17, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6GFvbcC004113 17, 19 -- for {microscopy-at-microscopy.com} ; Sat, 16 Jul 2005 10:57:37 -0500 17, 19 -- Received: from [192.168.1.100] (66-90-146-134.dyn.grandenetworks.net [66.90.146.134]) 17, 19 -- by mx2.lsn.net (8.13.0.Beta3/8.12.8) with ESMTP id j6GFvZef022611 17, 19 -- for {microscopy-at-microscopy.com} ; Sat, 16 Jul 2005 10:57:40 -0500 17, 19 -- Mime-Version: 1.0 (Apple Message framework v730) 17, 19 -- In-Reply-To: {200507151716.j6FHGuR9011532-at-ns.microscopy.com} 17, 19 -- References: {200507151716.j6FHGuR9011532-at-ns.microscopy.com} 17, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 17, 19 -- Message-Id: {472D4ED4-B5BB-474E-BDF9-BEE268C8A16A-at-eden.infohwy.com} 17, 19 -- Content-Transfer-Encoding: 7bit 17, 19 -- From: Roger Baker {rcbaker-at-eden.infohwy.com} 17, 19 -- Subject: Alternatives to diamond knives? 17, 19 -- Date: Sat, 16 Jul 2005 10:57:25 -0500 17, 19 -- To: microscopy-at-microscopy.com 17, 19 -- X-Mailer: Apple Mail (2.730) 17, 19 -- X-Antivirus: Scanned by Vexira Antivirus 1.0.6 ==============================End of - Headers==============================
Contact Art McCanna at MAS amccanna-at-mastest.com ; (770)866-3200; direct-(770)866-3212. MAS is exclusive factory representative for GATAN ion mills.
Vitaly Feingold SIA 2773 Heath Lane Duluth, GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {john.bonevich-at-nist.gov} To: {vitalylazar-at-att.net} Sent: Friday, July 15, 2005 1:13 PM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (luce-at-earthtech.org) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, July 17, 2005 at 16:06:51 ---------------------------------------------------------------------------
Email: luce-at-earthtech.org Name: George Luce
Organization: Earthtech Internaional, Inc.
Education: Graduate College
Location: Austin, Texas, USA
Question: We are looking for the electrical schematics for an International Scientific Instruments (ISI) Model 100B Scanning Electron Microscope. (~1979 vintage, probably made by Akashi)
Is this company still in business, or is there another resource for information about this SEM?
Thanks,
George Luce luce-at-earthtech.org
Earthtech International Inc. Austin, TX www.earthtech.org
the only comment that I can make is that sapphire knives were marketed a few (maybe 20) years ago but they were much more expensive than glass and not as hard as diamond so were never really that popular. I suppose in the real world a lot will depend on price and quality and whether there is a sufficiently large market, now.
I wish you luck.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: rcbaker-at-eden.infohwy.com
Hi Roger, It sounds interesting, keep us informed. Years ago, Diatome did market a Sapphire knife as a less-expensive alternative to diamonds. In my lab, they proved difficult to clean, and did not wear well enough to continue using them. They seem to have disappeared from the market, so my guess is that others had the same observations. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
==============================Original Headers============================== 1, 23 -- From lcgould-at-med.cornell.edu Mon Jul 18 09:11:25 2005 1, 23 -- Received: from smtp-in1.med.cornell.edu (smtp-in1.med.cornell.edu [140.251.1.25]) 1, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IEBPjw018439 1, 23 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 09:11:25 -0500 1, 23 -- Received: from mpx2.med.cornell.edu (pc113142-10.med.cornell.edu [140.251.11.119]) 1, 23 -- by smtp-in1.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6IEBNmQ207152 1, 23 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 10:11:23 -0400 1, 23 -- Received: from [140.251.145.131] by mpx2.med.cornell.edu 1, 23 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 23 -- with ESMTP id {0IJT00CEIU2Y6S90-at-mpx2.med.cornell.edu} for 1, 23 -- microscopy-at-microscopy.com; Mon, 18 Jul 2005 10:11:23 -0400 (EDT) 1, 23 -- Date: Mon, 18 Jul 2005 10:11:20 -0400 1, 23 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 23 -- Subject: Re: [Microscopy] Alternatives to diamond knives? 1, 23 -- In-reply-to: {200507161559.j6GFxljV005537-at-ns.microscopy.com} 1, 23 -- X-Sender: lcgould-at-pop.med.cornell.edu 1, 23 -- To: microscopy-at-microscopy.com 1, 23 -- Cc: rcbaker-at-eden.infohwy.com 1, 23 -- Message-id: {p0602040abf01684f55e8-at-[140.251.145.131]} 1, 23 -- MIME-version: 1.0 1, 23 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 23 -- References: {200507161559.j6GFxljV005537-at-ns.microscopy.com} 1, 23 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.7.18.11 ==============================End of - Headers==============================
The problem has been solved by using external fans running through tubes to the camera.
Roper and Cooke each have their own different designs for the fan. Each were extremely helpful with the retrofits.
Cooke runs the camera power through the fan; this assures that the fan must be on for the camera to run. Roper doesn't have this safety feature.
I would recommend not purchasing a camera with an on board fan. Always go for external cooling.
Thanks for the help from everybody who responded to my query ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/ **This electronic transmission contains information that is privileged.**
==============================Original Headers============================== 6, 24 -- From cammer-at-aecom.yu.edu Mon Jul 18 09:14:33 2005 6, 24 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) 6, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IEEXZ0022691 6, 24 -- for {microscopy-at-msa.microscopy.com} ; Mon, 18 Jul 2005 09:14:33 -0500 6, 24 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) 6, 24 -- by mailgw.aecom.yu.edu (8.12.11/8.12.11) with SMTP id j6IEE9B9030533 6, 24 -- for {microscopy-at-msa.microscopy.com} ; Mon, 18 Jul 2005 10:14:33 -0400 6, 24 -- Received: from post.aecom.yu.edu ([129.98.1.100]) 6, 24 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2005071810143320737 6, 24 -- for {microscopy-at-msa.microscopy.com} ; Mon, 18 Jul 2005 10:14:33 -0400 6, 24 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.30.137]) 6, 24 -- by post.aecom.yu.edu (Postfix) with ESMTP id E869A2FC0 6, 24 -- for {microscopy-at-msa.microscopy.com} ; Mon, 18 Jul 2005 10:14:32 -0400 (EDT) 6, 24 -- Message-Id: {5.2.1.1.2.20050718100956.033d4060-at-mailserver.aecom.yu.edu} 6, 24 -- X-Sender: cammer-at-mailserver.aecom.yu.edu 6, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 6, 24 -- Date: Mon, 18 Jul 2005 10:14:37 -0400 6, 24 -- To: microscopy-at-msa.microscopy.com 6, 24 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 6, 24 -- Subject: Re: vibrations in air cooled CCD cameras; follow-up 6, 24 -- In-Reply-To: {20050525210830.20166.qmail-at-web81301.mail.yahoo.com} 6, 24 -- References: {6667} 6, 24 -- Mime-Version: 1.0 6, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I have a student who wants to look at clusters of methanosarcina and methanosaeta in an aqueous solution. I have a materials background and have never worked with this type of sample. The student found a reference for preparing samples for electron microscopy which looks to me like it is for TEM. She is interested in what the clusters look like so is interested in retaining their relative position. I think SEM would be a good technique to see the 3D cluster if there is a method to prepare samples.
I have a FESEM which is high vacuum. I don't have easy access to an environmental SEM, so I'm looking for a technique to image these clusters in a high vacuum SEM.
Any suggestions for prep techniques and/or references?
Thanks,
Tom
------------------------------------------------------------------------ --------------------------------------------- Thomas M Murray email: murraytm-at-u.washington.edu Electron Microscopy Center Manager Phone: (206)543-2836 Materials Science & Engineering Fax: (206)543-3100 Box 352120 302 Roberts Hall Cell: (425)345-0083 University of Washington Seattle, WA 98195
==============================Original Headers============================== 7, 24 -- From murraytm-at-u.washington.edu Mon Jul 18 10:56:23 2005 7, 24 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134]) 7, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IFuMul003018 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 10:56:22 -0500 7, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 7, 24 -- by mxout1.cac.washington.edu (8.13.4+UW05.04/8.13.4+UW05.05) with ESMTP id j6IFuLsd018291 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 08:56:21 -0700 7, 24 -- Received: from [128.95.118.89] (tstoebe-mse.ad.engr.washington.edu [128.95.118.89]) 7, 24 -- (authenticated authid=murraytm) 7, 24 -- by smtp.washington.edu (8.13.4+UW05.04/8.13.4+UW05.05) with ESMTP id j6IFuLaF015330 7, 24 -- (version=TLSv1/SSLv3 cipher=RC4-SHA bits=128 verify=NOT) 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 08:56:21 -0700 7, 24 -- Mime-Version: 1.0 (Apple Message framework v733) 7, 24 -- In-Reply-To: {200507141333.j6EDXIb0014709-at-ns.microscopy.com} 7, 24 -- References: {200507141333.j6EDXIb0014709-at-ns.microscopy.com} 7, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 24 -- Message-Id: {9EC9D803-ACF3-4069-AA98-E78D3ABBACB2-at-u.washington.edu} 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- From: Tom Murray {murraytm-at-u.washington.edu} 7, 24 -- Subject: SEM Sample Prep Question 7, 24 -- Date: Mon, 18 Jul 2005 08:56:19 -0700 7, 24 -- To: microscopy-at-microscopy.com 7, 24 -- X-Mailer: Apple Mail (2.733) ==============================End of - Headers==============================
Al Coritz, Technical Engineer Electron Microscopy Sciences www.emsdiasum.com
----- Original Message ----- X-from: {murraytm-at-u.washington.edu} To: {Sampleprep-at-earthlink.net} Sent: Monday, July 18, 2005 11:56 AM
A colleague of mine is looking for an intensive TEM short course, 3-4 days, that covers theory and operation of TEM's, as well as interpretation of images, particularly as they are used in materials science. The course at Lehigh University for this year was just held, and waiting until next year is not a good option.
If you teach or know about such a course, please contact me offline and I will it forward the information.
Thanks very much,
--John Chandler Fort Collins, CO jchandler-at-ial-fa.com 970.217.1321
==============================Original Headers============================== 5, 22 -- From jchandler-at-ial-fa.com Mon Jul 18 11:51:41 2005 5, 22 -- Received: from sole.serverhost.net (sole.serverhost.net [216.71.84.188]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IGpfq9019267 5, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 11:51:41 -0500 5, 22 -- Received: from JohnChandler (proxy1.lsil.com [147.145.40.41]) 5, 22 -- by sole.serverhost.net (8.11.6/8.11.6) with SMTP id j6IGpeg09884 5, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 12:51:40 -0400 5, 22 -- Message-ID: {004c01c58bb8$d6f9ddf0$02000000-at-JohnChandler} 5, 22 -- Reply-To: "John Chandler" {jchandler-at-ial-fa.com} 5, 22 -- From: "John Chandler" {jchandler-at-ial-fa.com} 5, 22 -- To: {Microscopy-at-microscopy.com} 5, 22 -- Subject: Training: looking for TEM short course 5, 22 -- Date: Mon, 18 Jul 2005 10:50:43 -0600 5, 22 -- Organization: Insight Analytical Labs 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; 5, 22 -- charset="iso-8859-1" 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Priority: 3 5, 22 -- X-MSMail-Priority: Normal 5, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1506 5, 22 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.2800.1506 ==============================End of - Headers==============================
What's the procedure? Very likely, she can use it up through the 100% ethanol steps, whether or not it is for TEM. The critters can be filtered onto 0.22 micron membrane filters (the kind with nice, round pores, not a Millipore), and the filter critical point dried. An alternative to CPD is to air dry from HMDS (hexamethyldisilizane). Process the samples in 1.5 mL minifuge tubes, not on filters. After the last EtOH, go through a 2:1 1:1 1:2 EtOH:HMDS series, 3 changes in 100% HMDS, put sputter coated filters on the SEM stubs, drop the bugs in HMDS on the filters, and allow to air dry at room temp **do all this in a fume hood!**. See the U. Florida "tips and tricks" web site: http://www.biotech.ufl.edu/EM/tips/index.html
Or have a chat with the U. Washington biological EM people.
Phil
} I have a student who wants to look at clusters of methanosarcina and } methanosaeta in an aqueous solution. I have a materials background } and have never worked with this type of sample. The student found a } reference for preparing samples for electron microscopy which looks } to me like it is for TEM. She is interested in what the clusters } look like so is interested in retaining their relative position. I } think SEM would be a good technique to see the 3D cluster if there is } a method to prepare samples. } } I have a FESEM which is high vacuum. I don't have easy access to an } environmental SEM, so I'm looking for a technique to image these } clusters in a high vacuum SEM. } } Any suggestions for prep techniques and/or references? } } Thanks, } } Tom } } ------------------------------------------------------------------------ } --------------------------------------------- } Thomas M Murray email: } murraytm-at-u.washington.edu } Electron Microscopy Center Manager Phone: (206)543-2836 } Materials Science & Engineering Fax: (206)543-3100 } Box 352120 302 Roberts Hall Cell: (425)345-0083 } University of Washington } Seattle, WA 98195 } } } ==============================Original Headers============================== } 7, 24 -- From murraytm-at-u.washington.edu Mon Jul 18 10:56:23 2005 } 7, 24 -- Received: from mxout1.cac.washington.edu } (mxout1.cac.washington.edu [140.142.32.134]) } 7, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6IFuMul003018 } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 } 10:56:22 -0500 } 7, 24 -- Received: from smtp.washington.edu (smtp.washington.edu } [140.142.32.139]) } 7, 24 -- by mxout1.cac.washington.edu } (8.13.4+UW05.04/8.13.4+UW05.05) with ESMTP id j6IFuLsd018291 } 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA } bits=256 verify=OK) } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 } 08:56:21 -0700 } 7, 24 -- Received: from [128.95.118.89] } (tstoebe-mse.ad.engr.washington.edu [128.95.118.89]) } 7, 24 -- (authenticated authid=murraytm) } 7, 24 -- by smtp.washington.edu } (8.13.4+UW05.04/8.13.4+UW05.05) with ESMTP id j6IFuLaF015330 } 7, 24 -- (version=TLSv1/SSLv3 cipher=RC4-SHA bits=128 verify=NOT) } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 } 08:56:21 -0700 } 7, 24 -- Mime-Version: 1.0 (Apple Message framework v733) } 7, 24 -- In-Reply-To: {200507141333.j6EDXIb0014709-at-ns.microscopy.com} } 7, 24 -- References: {200507141333.j6EDXIb0014709-at-ns.microscopy.com} } 7, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 7, 24 -- Message-Id: {9EC9D803-ACF3-4069-AA98-E78D3ABBACB2-at-u.washington.edu} } 7, 24 -- Content-Transfer-Encoding: 7bit } 7, 24 -- From: Tom Murray {murraytm-at-u.washington.edu} } 7, 24 -- Subject: SEM Sample Prep Question } 7, 24 -- Date: Mon, 18 Jul 2005 08:56:19 -0700 } 7, 24 -- To: microscopy-at-microscopy.com } 7, 24 -- X-Mailer: Apple Mail (2.733) } ==============================End of - Headers==============================
-- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) http://www.ansci.wisc.edu/microscopy.htm
==============================Original Headers============================== 6, 27 -- From peoshel-at-wisc.edu Mon Jul 18 12:50:56 2005 6, 27 -- Received: from smtp7.wiscmail.wisc.edu (hagen.doit.wisc.edu [144.92.197.163]) 6, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IHounE027818 6, 27 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 12:50:56 -0500 6, 27 -- Received: from avs-daemon.smtp7.wiscmail.wisc.edu by smtp7.wiscmail.wisc.edu 6, 27 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 6, 27 -- id {0IJU00F5348SA4-at-smtp7.wiscmail.wisc.edu} for microscopy-at-microscopy.com; 6, 27 -- Mon, 18 Jul 2005 12:50:52 -0500 (CDT) 6, 27 -- Received: from [10.25.102.29] (ansci.wisc.edu [144.92.132.175]) 6, 27 -- by smtp7.wiscmail.wisc.edu 6, 27 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 6, 27 -- with ESMTPSA id {0IJU0085948QH8-at-smtp7.wiscmail.wisc.edu} for 6, 27 -- microscopy-at-microscopy.com; Mon, 18 Jul 2005 12:50:51 -0500 (CDT) 6, 27 -- Date: Mon, 18 Jul 2005 12:50:50 -0500 6, 27 -- From: Philip Oshel {peoshel-at-wisc.edu} 6, 27 -- Subject: Re: [Microscopy] SEM Sample Prep Question 6, 27 -- In-reply-to: {200507181557.j6IFvBgC004474-at-ns.microscopy.com} 6, 27 -- X-Sender: peoshel-at-wiscmail.wisc.edu 6, 27 -- To: microscopy-at-microscopy.com 6, 27 -- Message-id: {p05210601bf019a009786-at-[10.25.102.29]} 6, 27 -- MIME-version: 1.0 6, 27 -- Content-type: text/plain; format=flowed; charset=us-ascii 6, 27 -- Content-transfer-encoding: 7BIT 6, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.132.175 6, 27 -- X-Spam-PmxInfo: Server=avs-2, Version=4.7.1.128075, Antispam-Engine: 2.0.3.1, 6, 27 -- Antispam-Data: 2005.7.18.20, SenderIP=144.92.132.175 6, 27 -- References: {200507181557.j6IFvBgC004474-at-ns.microscopy.com} ==============================End of - Headers==============================
One potential use would be to section materials that might damage a diamond knife, but that could not be cut using a glass knife. For example, specimens containing potentially damaging inclusions as sand, metals, etc. or to cut hard botanical specimens. -- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
==============================Original Headers============================== 2, 18 -- From bozzola-at-siu.edu Mon Jul 18 14:49:53 2005 2, 18 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 2, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IJnrxe004719 2, 18 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 14:49:53 -0500 2, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 2, 18 -- by abbmta2.siu.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6IJnpvo026923 2, 18 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 14:49:52 -0500 (CDT) 2, 18 -- Mime-Version: 1.0 2, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu 2, 18 -- Message-Id: {p06110400bf01b80ddb8f-at-[131.230.177.142]} 2, 18 -- In-Reply-To: {200507161600.j6GG0kUa006196-at-ns.microscopy.com} 2, 18 -- References: {200507161600.j6GG0kUa006196-at-ns.microscopy.com} 2, 18 -- Date: Mon, 18 Jul 2005 14:49:50 -0500 2, 18 -- To: microscopy-at-microscopy.com 2, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 2, 18 -- Subject: Re: [Microscopy] Alternatives to diamond knives? 2, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 2, 18 -- X-MASF: 0.00% ==============================End of - Headers==============================
We have a Winter School from January 9 through 12. One half day on the 13th. Please go to our web site where you will find a description of last year's school. We will have the info for 2006 up in a few days. Let me know if you need more info. John Wheatley
} ---------- } From: jchandler-at-ial-fa.com } Reply To: microscopy-at-microscopy.com } Sent: Monday, July 18, 2005 9:53 AM } To: John Wheatley } Subject: [Microscopy] Training: looking for TEM short course } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } A colleague of mine is looking for an intensive TEM short course, 3-4 days, } that covers theory and operation of TEM's, as well as interpretation of } images, particularly as they are used in materials science. The course at } Lehigh University for this year was just held, and waiting until next year } is not a good option. } } If you teach or know about such a course, please contact me offline and I } will it forward the information. } } Thanks very much, } } --John Chandler } Fort Collins, CO } jchandler-at-ial-fa.com } 970.217.1321 } } } ==============================Original Headers============================== } 5, 22 -- From jchandler-at-ial-fa.com Mon Jul 18 11:51:41 2005 } 5, 22 -- Received: from sole.serverhost.net (sole.serverhost.net [216.71.84.188]) } 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IGpfq9019267 } 5, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 11:51:41 -0500 } 5, 22 -- Received: from JohnChandler (proxy1.lsil.com [147.145.40.41]) } 5, 22 -- by sole.serverhost.net (8.11.6/8.11.6) with SMTP id j6IGpeg09884 } 5, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 12:51:40 -0400 } 5, 22 -- Message-ID: {004c01c58bb8$d6f9ddf0$02000000-at-JohnChandler} } 5, 22 -- Reply-To: "John Chandler" {jchandler-at-ial-fa.com} } 5, 22 -- From: "John Chandler" {jchandler-at-ial-fa.com} } 5, 22 -- To: {Microscopy-at-microscopy.com} } 5, 22 -- Subject: Training: looking for TEM short course } 5, 22 -- Date: Mon, 18 Jul 2005 10:50:43 -0600 } 5, 22 -- Organization: Insight Analytical Labs } 5, 22 -- MIME-Version: 1.0 } 5, 22 -- Content-Type: text/plain; } 5, 22 -- charset="iso-8859-1" } 5, 22 -- Content-Transfer-Encoding: 7bit } 5, 22 -- X-Priority: 3 } 5, 22 -- X-MSMail-Priority: Normal } 5, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1506 } 5, 22 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.2800.1506 } ==============================End of - Headers============================== } }
==============================Original Headers============================== 8, 25 -- From JOHN.WHEATLEY-at-asu.edu Mon Jul 18 14:59:32 2005 8, 25 -- Received: from post5.inre.asu.edu (post5.inre.asu.edu [129.219.110.120]) 8, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IJxWeZ012538 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 14:59:32 -0500 8, 25 -- Received: from conversion.post5.inre.asu.edu by asu.edu (PMDF V6.1-1X6 #30769) 8, 25 -- id {0IJU00301A56P0-at-asu.edu} for microscopy-at-microscopy.com; Mon, 8, 25 -- 18 Jul 2005 12:58:18 -0700 (MST) 8, 25 -- Received: from EX1.asurite.ad.asu.edu (ex1.asurite.ad.asu.edu [129.219.10.211]) 8, 25 -- by asu.edu (PMDF V6.1-1X6 #30769) with ESMTP id {0IJU00LMFA56BR-at-asu.edu} for 8, 25 -- microscopy-at-microscopy.com; Mon, 18 Jul 2005 12:58:18 -0700 (MST) 8, 25 -- Date: Mon, 18 Jul 2005 12:58:18 -0700 8, 25 -- From: John Wheatley {JOHN.WHEATLEY-at-asu.edu} 8, 25 -- Subject: RE: [Microscopy] Training: looking for TEM short course 8, 25 -- To: microscopy-at-microscopy.com 8, 25 -- Message-id: {109BCD17C302C843A084EB9A5D45111D033E85E4-at-ex1.asurite.ad.asu.edu} 8, 25 -- MIME-version: 1.0 8, 25 -- X-MIMEOLE: Produced By Microsoft Exchange V6.0.6603.0 8, 25 -- Content-type: text/plain; charset=us-ascii 8, 25 -- Thread-Topic: [Microscopy] Training: looking for TEM short course 8, 25 -- Thread-Index: AcWLuUERqRrk+3XPQ/ertLOOe6eSagANYuxg 8, 25 -- Content-class: urn:content-classes:message 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6IJxWeZ012538 ==============================End of - Headers==============================
Thanks so much for getting back to me on your training course. I had looked at your website before polling the microscopy listserv, and it seemed to have lots of what is needed. ASU is one of the few centers for EM that I looked at before asking the question. I will pass along your information.
With best regards,
--John | jchandler-at-ial-fa.com | 970.217.1321
----- Original Message ----- X-from: {JOHN.WHEATLEY-at-asu.edu} To: {jchandler-at-ial-fa.com} Sent: Monday, July 18, 2005 1:59 PM
Dear Listers,
Below is a compilation of the replies I received to my recent question about Cu vs. Ni grids for immunolabeling. I usually don't do this without express permission, but most everybody posted to the list anyway, and I made sure to edit those that didn't. If it bothers anyone, let me know and I will never do it again.
In summary, it appears that copper can and will react with salts in buffers and other solutions, although some people still have decent luck. This problem can be ameliorated by using coated Cu grids and only wetting the coated side and by shortening incubation times
Also, Marc Pypaert had the interesting suggestion of just using Ni grids for everything, since they're not much more expensive, thereby avoiding the problem entirely. Any thoughts on this?
Many thanks to everyone who replied! I now have a much better response to our clients' questions on this matter (not to mention my own).
Cheers, Randy
Over the years I have frequently seen cases where the samples themselves have reacted with the copper grid. Two examples:
1) Looking at Fe-S precipitates in magnetotactic bacteria, when the precipitates were ugly and analyzed as copper sulphide. Our surmise was that the iron and copper had undergone ion exchange while the grid (and carbon film) was wet with the culture medium. When we used nickel grids, the precipitates were well formed and composed of iron and sulphur.
2) Looking at Si precipitates in Al-Si electronic bond wire. The specimens were prepared by embedding and microtoming, floating in DI water. The Si precipitates were surrounded by an ugly mess containing masses of copper. Again, presumed to me electrochemical reaction between the Al and the Cu grid, through the DI water medium, somehow preferentially at the Si precipitates. Again, use of Ni grids produced good pictures allowing us to characterize the precipitation.
Having said all that, we continue to default to use copper grids!
Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
-------------------------------------------
The main problem encountered with copper grids used for immunocytochemistry or immunogold labeling is the reaction of the copper with salts in the buffers used during labeling. This is a time-related reaction, and can usually be avoided by having short labeling runs and using grids with films on them. Gilder grids, available from major microscope supply vendors, are gold-coated copper grids, and are not that much more expensive than regular copper grids. These grids may be the variety used by researchers who have reported having no problems with their grids during their immuno runs. I usually use nickel grids for my work, but have used formvar-coated copper grids without problems for 1 day immuno runs so long as the film is intact and as long as I don't get clumsy and end up sinking my grids in my solutions (if I do sink them, I do a quick distilled water rinse, blot the back of the grids with filter paper until almost dry, then re-float them where I left off). As others have shared, the nickel grids are much sturdier, not too expensive, and are not a problem to handle as long as you use antimagnetic forceps. With the nickel grids, remember to correct for astigmatism in the TEM by using a hole in your sample on the nickel grid. This will accommodate for any inherent residual magnetic field in the grid itself.
Edward Haller Lab Manager, Diagnostic Electron Microscopy Lab University of South Florida Pathology Department Tampa, FL 33612
We have had problems with Cu grids, even Formvar coated ones. I have precoated copper grids with Parlodion by dipping, blotting and drying before use, with or with out a formvar coating. THat method seems to protect the copper from reacting with PBS or whatever. This is not original to me, but I cannot recall where I read this (among many other things I can no longer recall). I think it might have been one of those smart Swiss guys.
Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 -------------------------------------------------------------------
I had a bad experience with formvar-copper grids in immunogold experiment many years ago when incubating primary antibody overnight and the rest done on the following morning. I am using Nickel grids now -- no more problems.
Ann Fook Yang EM Unit/ Unite EM Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada 960 Carling Ave/960 Boul Carling Ottawa,Ontario/Ottawa, Ontario Canada K1A 0C6 ------------------------------------------------------------------------ ------------
recently i did a long ultracentrifuge run to load some 10S particles onto a formvar-copper grid. the grids reacted with the phosphate buffer over the 4 hour spin time. not a pretty picture - i had to go back to my collaborator and get fresh material so we could put it onto formvar-nickel grids. wonderful gentleman.
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 ----------------------------------------------------------------
We used Cu grids for many years. Normally we use formvar + carbon coated grids for ICC since we have a lot less loss of sections. We also tend to do long incubations ...usually overnight. This is time efficient and also lets us use highly diluted antibody so also minimize background due to cross-reactions from contaminants in polyclonal antibodies.
Occasionally we would have a reaction due to copper oxidizing with resultant green solution. I attributed this to salts or traces of Tween 20 in the buffer and breaks in the coating exposing the Cu. As far as I can see, this is the only reason for not using Cu grids if you use coated grids. We have switched for the most part to using coated Ni grids to avoid this problem.
On the other hand, occasionally we need to do a double labeling using uncoated grids and both sides of the sections. I would hesitate to use Cu for this and prefer Ni. Gold would work but is both more expensive and more delicate than Ni grids.
Debby Sherman, Manager Life Science Microscopy Facility Purdue University S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 ------------------------------------------------------------------------ -
X-from my experience, coated copper grids will be fine for most (if not all) immunolabelling, providing the solutions do not wet the uncoated side of the grid. Uncoated copper grids always have been fine when I've used PBS.
However, copper grids (uncoated - or the uncoated side) usually will react with Tris-HCl buffers and some of the high salt buffer formulations that I've used. Longer incubation times (eg. overnight) cause more reaction with the grid than shorter times (eg. 60mins) - so you may be able to use copper grids without problems if incubations are short, even with buffer formulations that might react with the grid if left for longer periods. It also is more difficult to prevent wetting of the reverse (uncoated) side of coated copper grids if incubation times are long, and particularly if wetting agents are used in the buffer formulation.
When I started immunolabelling in the early 1980s - and hadn't yet heard about the use of nickel grids - I used uncoated copper grids (and PBS) for years without problems. My current lab generally uses a high salt Tris-HCl buffer which includes a small amount of Tween 20, so we routinely use nickel grids for immunolabelling. Nickel grids do have some disadvantages (eg. charge), but we run a multi-user facility, often training students or other inexperienced users, we've found nickel the best option to overcome potential "grid-reaction" problems. However, nickel grids certainly aren't essential for successful immunolabelling - you just need to be aware of the possible problems.
Dr Deborah Stenzel Analytical Electron Microscopy Facility Queensland University of Technology GPO Box 2434 Brisbane 4001 Australia
------------------------------------------------
I know (from sad experience) that Tris buffers will etch Cu grids - the antibody droplets turn a lovely blue and there is gunk all over the sections. I use Ni grids, only because I prefer Tris buffers (no real reason why, I guess) for IEM and my current PI cringes at buying Au grids. I know one person who does all of her labelling in phosphate buffer and uses Cu grids with no precipitate problems.
As for the other reasons you Googled....I've heard them, but never seen any documentation nor had personal experience.
Can't wait to hear if anyone has actual evidence for some of the other no-Cu reasons!
Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 ----------------------------------------------------------------
Hi Randy, I had problems with Cu grids when incubating sections for long periods - overnight for instance. The copper would react with whatever was available and form green-blue salts. Not unexpected. We switched to nickel grids for a while but they charge, gold but they are delicate...by this time we had shortened the incubation times used, and tried copper again... And had no problem using coated grids and incubation times of an hour or so. Grids are floated on drops of media so that only the coated side is wetted - I don't know if that is important.
Dr SJ Stowe Facility Coordinator ANU Electron Microscopy Unit ANU CRICOS#00120C
I only ran into the "use only Ni or Au grids" rule here, in my current job, and my predecessor certainly produced some superb images showing gold labelling of TEM sections. Blissfully unaware of this rule, I had been using copper grids for all EM immunolabelling. To get good labelling of one particular structure, which is about 40 nm diameter, I used uncoated thin-bar Cu grids so I could get labelling on both sides of the section - seemed to work just fine. I'll be interested to see the responses to your question.
Dr. Rosemary White Microscopy Centre CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 54, 25 -- From TindallR-at-missouri.edu Mon Jul 18 16:21:32 2005 54, 25 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 54, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6ILLW7R028963 54, 25 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 16:21:32 -0500 54, 25 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 54, 25 -- Mon, 18 Jul 2005 16:21:31 -0500 54, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 54, 25 -- Content-class: urn:content-classes:message 54, 25 -- MIME-Version: 1.0 54, 25 -- Content-Type: text/plain; 54, 25 -- charset="us-ascii" 54, 25 -- Subject: TEM: Immunocytochemistry and Cu grids 54, 25 -- Date: Mon, 18 Jul 2005 16:21:30 -0500 54, 25 -- Message-ID: {BA876152E8653240BE8572E897083EE79802FD-at-UM-EMAIL09.um.umsystem.edu} 54, 25 -- X-MS-Has-Attach: 54, 25 -- X-MS-TNEF-Correlator: 54, 25 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids 54, 25 -- Thread-Index: AcWL3qpvaFt7UpRlRY2LVYYS/0TUZg== 54, 25 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 54, 25 -- To: {microscopy-at-microscopy.com} 54, 25 -- Cc: "Jensen, Cheryl A." {JensenC-at-missouri.edu} , 54, 25 -- "Chance, Deborah L." {ChanceD-at-health.missouri.edu} 54, 25 -- X-OriginalArrivalTime: 18 Jul 2005 21:21:31.0576 (UTC) FILETIME=[AAE06380:01C58BDE] 54, 25 -- Content-Transfer-Encoding: 8bit 54, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6ILLW7R028963 ==============================End of - Headers==============================
Dear Roger I am not sure is tungsten carbide is similar to SiC or not. My experience with tungsten carbide basically was negative - this knife starts scratching the surface after just 50+ 0.5 um sections of standard plastic embedded tissue (no hard inclusions etc). As manufacturer explained to me it's because of polycrystalline nature of the material - small crystals just became loose and left the edge creating ruff surface. From this point of view, amorphous (like glass) or monocrystal (like diamond) material is preferable for good sections. Sapphire (hardness is 9) knifes were on the market for while without great success. From economical point of view, tungsten carbide knifes were also not so good - $100 each with ability to produce only 50+ good sections. If SiC acts in the similar way, then it would be difficult to use it in EM applications. Another aspect of using exotic materials - is it hydrophilic or hydrophobic? Could you use water with them? How easy to clean it up and handle? Sergey
At 07:00 AM 7/18/2005, you wrote:
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==============================Original Headers============================== 6, 25 -- From sryazant-at-ucla.edu Mon Jul 18 17:44:31 2005 6, 25 -- Received: from smtp-9.smtp.ucla.edu (smtp-9.smtp.ucla.edu [169.232.48.137]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6IMiV81005437 6, 25 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 17:44:31 -0500 6, 25 -- Received: from mail.ucla.edu (mail.ucla.edu [169.232.47.141]) 6, 25 -- by smtp-9.smtp.ucla.edu (8.13.4/8.13.4) with ESMTP id j6IMiTCN030258 6, 25 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 15:44:29 -0700 6, 25 -- Received: from kopoba.ucla.edu (ts17-67.dialup.bol.ucla.edu [169.232.230.143]) 6, 25 -- (authenticated bits=0) 6, 25 -- by mail.ucla.edu (8.13.4/8.13.4) with ESMTP id j6IMiHs9001337 6, 25 -- (version=TLSv1/SSLv3 cipher=DES-CBC3-SHA bits=168 verify=NOT) 6, 25 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 15:44:21 -0700 6, 25 -- Message-Id: {6.1.2.0.2.20050718152757.032ce900-at-mail.ucla.edu} 6, 25 -- X-Sender: sryazant-at-mail.ucla.edu 6, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 25 -- Date: Mon, 18 Jul 2005 15:44:06 -0700 6, 25 -- To: microscopy-at-microscopy.com 6, 25 -- From: Sergey {sryazant-at-ucla.edu} 6, 25 -- Subject: Re: [Microscopy] Re: Alternatives to diamond knives? 6, 25 -- In-Reply-To: {200507181400.j6IE0bdx012784-at-ns.microscopy.com} 6, 25 -- References: {200507181400.j6IE0bdx012784-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 25 -- X-Probable-Spam: no 6, 25 -- X-Scanned-By: smtp.ucla.edu on 169.232.48.137 ==============================End of - Headers==============================
On Jul 18, 2005, at 5:48 PM, sryazant-at-ucla.edu wrote: } } Dear Roger } I am not sure is tungsten carbide is similar to SiC or not. My } experience } with tungsten carbide basically was negative - this knife starts } scratching } the surface after just 50+ 0.5 um sections of standard plastic } embedded } tissue (no hard inclusions etc). As manufacturer explained to me it's } because of polycrystalline nature of the material - small crystals } just } became loose and left the edge creating ruff surface. From this } point of } view, amorphous (like glass) or monocrystal (like diamond) material is } preferable for good sections. Sapphire (hardness is 9) knifes were } on the } market for while without great success. From economical point of } view, } tungsten carbide knifes were also not so good - $100 each with } ability to } produce only 50+ good sections. If SiC acts in the similar way, } then it } would be difficult to use it in EM applications. Another aspect of } using } exotic materials - is it hydrophilic or hydrophobic? Could you use } water } with them? How easy to clean it up and handle? Sergey
Thanks everyone, I've gotten lots of helpful advice and comments, so I'll try to sum up my conclusions.
Some background: Silicon carbide (SiC) is produced by a very old company Washington Mills located near Niagara Falls but production is in Illinois. Blessed folks; they sent me a sample of crystals for free.
It is made in tonnage quantities as clusters of glossy flat hexagonal black crystals up to a few centimeters across, and which are actually deep transparent blue and are crushed for abrasives and metallurgy. The cost of this industrial crystalline material is negligible. The Cree Corporation in NC makes also clear white SiC crystals and wafers for a fairly high price, but only sells them already faceted into clear diamond substitutes for jewelry. Such clear SiC crystals are termed "moissenite".
SiC crystals are very hard and brittle and the edge breaks when you try to sharpen them into knives using anything resembling normal gem faceting methods. But knives can be made using the proper lapping technique and the appropriate grades of diamond powder. Here is an interesting link on modern diamond powder technology:
At any rate, besides glass, only hard single crystals of diamond, sapphire, and silicon carbide seem to be appropriate for making ultramicrotome knives, which by their nature demand a perfectly homogeneous material. Sapphire knives were sold at one time by Diatome but the price was reputedly about $500 apiece and they became dull, unlike diamond knives, so the economics was questionable.
My SiC knives become dull after a few hundred slices, probably depending on the the hardness of the embedding plastic (glycol methacrylate works well), but the raw materials are inexpensive and my lapping machine only cost about $50 to build, so the labor of making them is inherently the major cost.
Since SiC crystals are difficult to sharpen without breaking the edge, I assume the exact same lapping techniques would also be appropriate for sapphire, which is softer tougher and less brittle than SiC; the latter are the very devil to sharpen without breaking. I learned how to make them through sheer stubbornness in order to make many serial sections with my Porter Blum MT-2 without paying $1000+ for a diamond knife.
I can only guess at the durability of sapphire knives, but the fact that they were once offered indicates that they might still be a viable alternative for some applications if they were only available at a lower cost.
My SiC crystals are epoxyed in a slot cut in the apex of a steel holder the same size and shape as a right angle glass knife. The clean crystals are hydrophilic (I assume the exposed surface layer at the atomic level is silicon oxide). I clean them by wiping the edge sideways with Teflon and don't have many wetting problems.
My conclusion is that I don't much want to go the trouble of trying to make money patenting, licensing and selling knives, but that I should publish my technique of making them, most likely in the Review of Scientific Instruments, and see if the world pays attention. I don't think the method of sharpening hard crystals to give very sharp edges has ever been published; I can't find it in the scientific literature. I seriously doubt it would work for diamonds without modification.
The only possible drawback to open publication is that no one maker could make a lot of money making them, and thus might not offer them, assuming they prove to be a modestly useful alternative to diamonds.
I assume that maybe the Chinese could sell disposable knives for $20 or so and they might catch on for student use and low budget and occasional use. Glass knives are clearly sharper for a few dozen sections and diamond knives clearly last longer.
-- Roger Baker Jr. 1303 Bentwood, Austin Tx, 78722
==============================Original Headers============================== 29, 19 -- From rcbaker-at-eden.infohwy.com Mon Jul 18 21:18:00 2005 29, 19 -- Received: from mx3.lsn.net (mx3.lsn.net [66.90.130.75]) 29, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6J2Hw75015970 29, 19 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 21:17:59 -0500 29, 19 -- Received: from [192.168.1.100] (66-90-146-134.dyn.grandenetworks.net [66.90.146.134]) 29, 19 -- by mx3.lsn.net (8.13.0.Beta3/8.12.8) with ESMTP id j6J2HkKD013027 29, 19 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 21:17:50 -0500 29, 19 -- Mime-Version: 1.0 (Apple Message framework v733) 29, 19 -- In-Reply-To: {200507182248.j6IMmje4011361-at-ns.microscopy.com} 29, 19 -- References: {200507182248.j6IMmje4011361-at-ns.microscopy.com} 29, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 29, 19 -- Message-Id: {0FA04694-AC3E-4D7F-894D-13AD154CAF36-at-eden.infohwy.com} 29, 19 -- Content-Transfer-Encoding: 7bit 29, 19 -- From: Roger Baker {rcbaker-at-eden.infohwy.com} 29, 19 -- Subject: Re: [Microscopy] Alternatives to diamond knives? 29, 19 -- Date: Mon, 18 Jul 2005 21:17:44 -0500 29, 19 -- To: microscopy-at-microscopy.com 29, 19 -- X-Mailer: Apple Mail (2.733) 29, 19 -- X-Antivirus: Scanned by Vexira Antivirus 1.0.6 ==============================End of - Headers==============================
You should be aware that recently, gem-quality silicon carbide has been under production and is being marketed under its mineralogical name as "synthetic moissanite". This grade is significantly more free of defects and might yield a much more durable edge. I don't know the economics of producing a knife edge from this stock but I suspect that the material price is not prohibitively high, given the prices of stones that are being sold as jewelry and the usual mark-up in that field.
Sincerely,
John Twilley
rcbaker-at-eden.infohwy.com wrote:
} Thanks everyone, I've gotten lots of helpful advice and comments, so } I'll try to sum up my conclusions. } } Some background: Silicon carbide (SiC) is produced by a very old } company Washington Mills located near Niagara Falls but production is } in Illinois. Blessed folks; they sent me a sample of crystals for free. } } {http://www.medibix.com/company.jsp?company_id=10001791} } } It is made in tonnage quantities as clusters of glossy flat hexagonal } black crystals up to a few centimeters across, and which are actually } deep transparent blue and are crushed for abrasives and metallurgy. } The cost of this industrial crystalline material is negligible. The } Cree Corporation in NC makes also clear white SiC crystals and wafers } for a fairly high price, but only sells them already faceted into } clear diamond substitutes for jewelry. Such clear SiC crystals are } termed "moissenite". } } SiC crystals are very hard and brittle and the edge breaks when you } try to sharpen them into knives using anything resembling normal gem } faceting methods. But knives can be made using the proper lapping } technique and the appropriate grades of diamond powder. Here is an } interesting link on modern diamond powder technology: } } {http://www.ceramicindustry.com/CDA/ArticleInformation/features/ } BNP__Features__Item/0,2710,152388,00.html} } } At any rate, besides glass, only hard single crystals of diamond, } sapphire, and silicon carbide seem to be appropriate for making } ultramicrotome knives, which by their nature demand a perfectly } homogeneous material. Sapphire knives were sold at one time by } Diatome but the price was reputedly about $500 apiece and they became } dull, unlike diamond knives, so the economics was questionable. } } My SiC knives become dull after a few hundred slices, probably } depending on the the hardness of the embedding plastic (glycol } methacrylate works well), but the raw materials are inexpensive and } my lapping machine only cost about $50 to build, so the labor of } making them is inherently the major cost. } } Since SiC crystals are difficult to sharpen without breaking the } edge, I assume the exact same lapping techniques would also be } appropriate for sapphire, which is softer tougher and less brittle } than SiC; the latter are the very devil to sharpen without breaking. } I learned how to make them through sheer stubbornness in order to } make many serial sections with my Porter Blum MT-2 without paying } $1000+ for a diamond knife. } } I can only guess at the durability of sapphire knives, but the fact } that they were once offered indicates that they might still be a } viable alternative for some applications if they were only available } at a lower cost. } } My SiC crystals are epoxyed in a slot cut in the apex of a steel } holder the same size and shape as a right angle glass knife. The } clean crystals are hydrophilic (I assume the exposed surface layer at } the atomic level is silicon oxide). I clean them by wiping the edge } sideways with Teflon and don't have many wetting problems. } } My conclusion is that I don't much want to go the trouble of trying } to make money patenting, licensing and selling knives, but that I } should publish my technique of making them, most likely in the Review } of Scientific Instruments, and see if the world pays attention. I } don't think the method of sharpening hard crystals to give very sharp } edges has ever been published; I can't find it in the scientific } literature. I seriously doubt it would work for diamonds without } modification. } } The only possible drawback to open publication is that no one maker } could make a lot of money making them, and thus might not offer them, } assuming they prove to be a modestly useful alternative to diamonds. } } I assume that maybe the Chinese could sell disposable knives for $20 } or so and they might catch on for student use and low budget and } occasional use. Glass knives are clearly sharper for a few dozen } sections and diamond knives clearly last longer. } } -- Roger Baker Jr. } 1303 Bentwood, } Austin Tx, 78722 } } } ==============================Original Headers============================== } 29, 19 -- From rcbaker-at-eden.infohwy.com Mon Jul 18 21:18:00 2005 } 29, 19 -- Received: from mx3.lsn.net (mx3.lsn.net [66.90.130.75]) } 29, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6J2Hw75015970 } 29, 19 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 21:17:59 -0500 } 29, 19 -- Received: from [192.168.1.100] (66-90-146-134.dyn.grandenetworks.net [66.90.146.134]) } 29, 19 -- by mx3.lsn.net (8.13.0.Beta3/8.12.8) with ESMTP id j6J2HkKD013027 } 29, 19 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 21:17:50 -0500 } 29, 19 -- Mime-Version: 1.0 (Apple Message framework v733) } 29, 19 -- In-Reply-To: {200507182248.j6IMmje4011361-at-ns.microscopy.com} } 29, 19 -- References: {200507182248.j6IMmje4011361-at-ns.microscopy.com} } 29, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 29, 19 -- Message-Id: {0FA04694-AC3E-4D7F-894D-13AD154CAF36-at-eden.infohwy.com} } 29, 19 -- Content-Transfer-Encoding: 7bit } 29, 19 -- From: Roger Baker {rcbaker-at-eden.infohwy.com} } 29, 19 -- Subject: Re: [Microscopy] Alternatives to diamond knives? } 29, 19 -- Date: Mon, 18 Jul 2005 21:17:44 -0500 } 29, 19 -- To: microscopy-at-microscopy.com } 29, 19 -- X-Mailer: Apple Mail (2.733) } 29, 19 -- X-Antivirus: Scanned by Vexira Antivirus 1.0.6 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 18 -- From jtwilley-at-sprynet.com Mon Jul 18 22:24:32 2005 9, 18 -- Received: from pop-satin.atl.sa.earthlink.net (pop-satin.atl.sa.earthlink.net [207.69.195.63]) 9, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6J3OW7G024406 9, 18 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 22:24:32 -0500 9, 18 -- Received: from pool-68-161-123-16.ny325.east.verizon.net ([68.161.123.16] helo=sprynet.com) 9, 18 -- by pop-satin.atl.sa.earthlink.net with esmtp (Exim 3.36 #10) 9, 18 -- id 1Duiid-0002BG-00; Mon, 18 Jul 2005 23:24:31 -0400 9, 18 -- Message-ID: {42DC7B7B.2CBEC0B5-at-sprynet.com} 9, 18 -- Date: Tue, 19 Jul 2005 00:03:07 -0400 9, 18 -- From: John Twilley {jtwilley-at-sprynet.com} 9, 18 -- X-Mailer: Mozilla 4.04 [en] (Win95; U) 9, 18 -- MIME-Version: 1.0 9, 18 -- To: microscopy-at-microscopy.com, rcbaker-at-eden.infohwy.com 9, 18 -- Subject: Re: [Microscopy] Re: Alternatives to diamond knives? 9, 18 -- References: {200507190218.j6J2IJo0016245-at-ns.microscopy.com} 9, 18 -- X-Corel-MessageType: EMail 9, 18 -- Content-Type: text/plain; charset=us-ascii 9, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I followed this thread by curiosity as I knew that copper is not always a good solution. And it was good to receive your message so that I know that there was no response that we missed. I think that the idea of giving a summary of the answers to the list, is a very respectful manner to contribute to its usefulness. Thanks a lot,
Sousan
TindallR-at-missouri.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } Below is a compilation of the replies I received to my recent question } about Cu vs. Ni grids for immunolabeling. I usually don't do this } without express permission, but most everybody posted to the list } anyway, and I made sure to edit those that didn't. If it bothers } anyone, let me know and I will never do it again. } } In summary, it appears that copper can and will react with salts in } buffers and other solutions, although some people still have decent } luck. This problem can be ameliorated by using coated Cu grids and only } wetting the coated side and by shortening incubation times } } Also, Marc Pypaert had the interesting suggestion of just using Ni grids } for everything, since they're not much more expensive, thereby avoiding } the problem entirely. Any thoughts on this? } } Many thanks to everyone who replied! I now have a much better response } to our clients' questions on this matter (not to mention my own). } } Cheers, } Randy } } } Over the years I have frequently seen cases where the samples themselves } have reacted with the copper grid. Two examples: } } 1) Looking at Fe-S precipitates in magnetotactic bacteria, when the } precipitates were ugly and analyzed as copper sulphide. Our surmise was } that the iron and copper had undergone ion exchange while the grid (and } carbon film) was wet with the culture medium. When we used nickel } grids, the precipitates were well formed and composed of iron and } sulphur. } } 2) Looking at Si precipitates in Al-Si electronic bond wire. The } specimens were prepared by embedding and microtoming, floating in DI } water. The Si precipitates were surrounded by an ugly mess containing } masses of copper. Again, presumed to me electrochemical reaction } between the Al and the Cu grid, through the DI water medium, somehow } preferentially at the Si precipitates. Again, use of Ni grids produced } good pictures allowing us to characterize the precipitation. } } Having said all that, we continue to default to use copper grids! } } Anthony J. Garratt-Reed, M.A., D.Phil. } MIT Room 13-1027 } 77 Massachusetts Avenue } Cambridge, MA 02139-4307 } USA } } } ------------------------------------------- } } The main problem encountered with copper grids used for } immunocytochemistry or immunogold labeling is the reaction of the copper } with salts in the buffers used during labeling. This is a time-related } reaction, and can usually be avoided by having short labeling runs and } using grids with films on them. Gilder grids, available from major } microscope supply vendors, are gold-coated copper grids, and are not } that much more expensive than regular copper grids. These grids may be } the variety used by researchers who have reported having no problems } with their grids during their immuno runs. } I usually use nickel grids for my work, but have used } formvar-coated copper grids without problems for 1 day immuno runs so } long as the film is intact and as long as I don't get clumsy and end up } sinking my grids in my solutions (if I do sink them, I do a quick } distilled water rinse, blot the back of the grids with filter paper } until almost dry, then re-float them where I left off). As others have } shared, the nickel grids are much sturdier, not too expensive, and are } not a problem to handle as long as you use antimagnetic forceps. With } the nickel grids, remember to correct for astigmatism in the TEM by } using a hole in your sample on the nickel grid. This will accommodate } for any inherent residual magnetic field in the grid itself. } } Edward Haller } Lab Manager, Diagnostic Electron Microscopy Lab University of South } Florida Pathology Department Tampa, FL 33612 } } ------------------------------------------------------------ } } We have had problems with Cu grids, even Formvar coated ones. I have } precoated copper grids with Parlodion by dipping, blotting and drying } before use, with or with out a formvar coating. THat method seems to } protect the copper from reacting with PBS or whatever. This is not } original to me, but I cannot recall where I read this (among many other } things I can no longer recall). I think it might have been one of those } smart Swiss guys. } } Gregory W. Erdos, Ph.D. } Assistant Director, Biotechnology Program Scientific Director, Electron } Microscopy P.O. Box 118525 } 217 Carr Hall } University of Florida } Gainesville, FL 32611 } ------------------------------------------------------------------- } } I had a bad experience with formvar-copper grids in immunogold } experiment many years ago when incubating primary antibody overnight and } the rest done on the following morning. I am using Nickel grids now -- } no more problems. } } Ann Fook Yang } EM Unit/ Unite EM } Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada } 960 Carling Ave/960 Boul Carling } Ottawa,Ontario/Ottawa, Ontario } Canada } K1A 0C6 } ------------------------------------------------------------------------ } ------------ } } recently i did a long ultracentrifuge run to load some 10S particles } onto a formvar-copper grid. the grids reacted with the phosphate buffer } over the 4 hour spin time. not a pretty picture - i had to go back to } my collaborator and get fresh material so we could put it onto } formvar-nickel grids. wonderful gentleman. } } } Paul R. Hazelton, PhD } Electron Microscope Unit } University of Manitoba } Department of Medical Microbiology } 531 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 0W3 } ---------------------------------------------------------------- } } We used Cu grids for many years. Normally we use formvar + carbon } coated grids for ICC since we have a lot less loss of sections. We also } tend to do long incubations ...usually overnight. This is time } efficient and also lets us use highly diluted antibody so also minimize } background due to cross-reactions from contaminants in polyclonal } antibodies. } } Occasionally we would have a reaction due to copper oxidizing with } resultant green solution. I attributed this to salts or traces of Tween } 20 in the buffer and breaks in the coating exposing the Cu. As far as I } can see, this is the only reason for not using Cu grids if you use } coated grids. We have switched for the most part to using coated Ni } grids to avoid this problem. } } On the other hand, occasionally we need to do a double labeling using } uncoated grids and both sides of the sections. I would hesitate to use } Cu for this and prefer Ni. Gold would work but is both more expensive } and more delicate than Ni grids. } } Debby Sherman, Manager } Life Science Microscopy Facility } Purdue University } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } ------------------------------------------------------------------------ } - } } X-from my experience, coated copper grids will be fine for most (if not } all) immunolabelling, providing the solutions do not wet the uncoated } side of the grid. Uncoated copper grids always have been fine when I've } used PBS. } } However, copper grids (uncoated - or the uncoated side) usually will } react with Tris-HCl buffers and some of the high salt buffer } formulations that I've used. Longer incubation times (eg. overnight) } cause more reaction with the grid than shorter times (eg. 60mins) - so } you may be able to use copper grids without problems if incubations are } short, even with buffer formulations that might react with the grid if } left for longer periods. It also is more difficult to prevent wetting } of the reverse (uncoated) side of coated copper grids if incubation } times are long, and particularly if wetting agents are used in the } buffer formulation. } } When I started immunolabelling in the early 1980s - and hadn't yet heard } about the use of nickel grids - I used uncoated copper grids (and PBS) } for years without problems. My current lab generally uses a high salt } Tris-HCl buffer which includes a small amount of Tween 20, so we } routinely use nickel grids for immunolabelling. Nickel grids do have } some disadvantages (eg. charge), but we run a multi-user facility, often } training students or other inexperienced users, we've found nickel the } best option to overcome potential "grid-reaction" problems. However, } nickel grids certainly aren't essential for successful immunolabelling - } you just need to be aware of the possible problems. } } Dr Deborah Stenzel } Analytical Electron Microscopy Facility } Queensland University of Technology } GPO Box 2434 } Brisbane 4001 } Australia } } ------------------------------------------------ } } I know (from sad experience) that Tris buffers will etch Cu grids - the } antibody droplets turn a lovely blue and there is gunk all over the } sections. I use Ni grids, only because I prefer Tris buffers (no real } reason why, I guess) for IEM and my current PI cringes at buying Au } grids. } I know one person who does all of her labelling in phosphate buffer and } uses Cu grids with no precipitate problems. } } As for the other reasons you Googled....I've heard them, but never seen } any documentation nor had personal experience. } } Can't wait to hear if anyone has actual evidence for some of the other } no-Cu reasons! } } Tamara Howard } Department of Cell Biology and Physiology } University of New Mexico - Health Sciences Center } Albuquerque, NM 87131 } ---------------------------------------------------------------- } } Hi Randy, } I had problems with Cu grids when incubating sections for long periods - } overnight for instance. The copper would react with whatever was } available and form green-blue salts. Not unexpected. We switched to } nickel grids for a while but they charge, gold but they are } delicate...by this time we had shortened the incubation times used, and } tried copper again... And had no problem using coated grids and } incubation times of an hour or so. Grids are floated on drops of media } so that only the coated side is wetted - I don't know if that is } important. } } Dr SJ Stowe } Facility Coordinator } ANU Electron Microscopy Unit } ANU CRICOS#00120C } } } I only ran into the "use only Ni or Au grids" rule here, in my current } job, and my predecessor certainly produced some superb images showing } gold labelling of TEM sections. Blissfully unaware of this rule, I had } been using copper grids for all EM immunolabelling. To get good } labelling of one particular structure, which is about 40 nm diameter, I } used uncoated thin-bar Cu grids so I could get labelling on both sides } of the section - seemed to work just fine. I'll be interested to see } the responses to your question. } } Dr. Rosemary White } Microscopy Centre } CSIRO Plant Industry } GPO Box 1600 } Canberra, ACT 2601 } Australia } } } } } } } } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } } } } } ==============================Original Headers============================== } 54, 25 -- From TindallR-at-missouri.edu Mon Jul 18 16:21:32 2005 } 54, 25 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) } 54, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6ILLW7R028963 } 54, 25 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 16:21:32 -0500 } 54, 25 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 54, 25 -- Mon, 18 Jul 2005 16:21:31 -0500 } 54, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 54, 25 -- Content-class: urn:content-classes:message } 54, 25 -- MIME-Version: 1.0 } 54, 25 -- Content-Type: text/plain; } 54, 25 -- charset="us-ascii" } 54, 25 -- Subject: TEM: Immunocytochemistry and Cu grids } 54, 25 -- Date: Mon, 18 Jul 2005 16:21:30 -0500 } 54, 25 -- Message-ID: {BA876152E8653240BE8572E897083EE79802FD-at-UM-EMAIL09.um.umsystem.edu} } 54, 25 -- X-MS-Has-Attach: } 54, 25 -- X-MS-TNEF-Correlator: } 54, 25 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids } 54, 25 -- Thread-Index: AcWL3qpvaFt7UpRlRY2LVYYS/0TUZg== } 54, 25 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 54, 25 -- To: {microscopy-at-microscopy.com} } 54, 25 -- Cc: "Jensen, Cheryl A." {JensenC-at-missouri.edu} , } 54, 25 -- "Chance, Deborah L." {ChanceD-at-health.missouri.edu} } 54, 25 -- X-OriginalArrivalTime: 18 Jul 2005 21:21:31.0576 (UTC) FILETIME=[AAE06380:01C58BDE] } 54, 25 -- Content-Transfer-Encoding: 8bit } 54, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6ILLW7R028963 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 20 -- From sousan.abolhassani-at-psi.ch Tue Jul 19 01:34:57 2005 5, 20 -- Received: from psi11.psi.ch (psi11.psi.ch [129.129.190.111]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6J6YuAl009619 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 01:34:56 -0500 5, 20 -- Received: from 129.129.190.113 by psi11.psi.ch (InterScan E-Mail VirusWall NT); Tue, 19 Jul 2005 08:34:54 +0200 5, 20 -- Received: from [129.129.237.86] (PC3870.psi.ch [129.129.237.86]) by psi13.psi.ch with SMTP (Microsoft Exchange Internet Mail Service Version 5.5.2657.72) 5, 20 -- id JGAVKF5S; Tue, 19 Jul 2005 08:34:53 +0200 5, 20 -- Message-ID: {42DC9F0D.7050109-at-psi.ch} 5, 20 -- Date: Tue, 19 Jul 2005 08:34:53 +0200 5, 20 -- From: Sousan Abolhassani {sousan.abolhassani-at-psi.ch} 5, 20 -- Organization: Paul Scherrer Institut 5, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.8) Gecko/20050511 5, 20 -- X-Accept-Language: en-us, en 5, 20 -- MIME-Version: 1.0 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids 5, 20 -- References: {200507182124.j6ILOfrB002304-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200507182124.j6ILOfrB002304-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I need to prep some samples for SEM, but I'm not sure of the best way to go about it.
We have some bacteria that normally grow in a type of liquid nutrient medium as free, discrete and seperate motile cells. However, we have come across a strain that forms into 'mats' on the bottom of the culture vessel. It is these that we would like to examine by SEM.
The problem is that the 'mats' are not like a normal biofilm in that they are not strongly adherant to a solid surface, and the mat is not very robust. The mats will 'float' off the bottom of the culture vessel if they are disturbed and they will fragment into small flakes if the vessel is shaken or swirled. The fragments are usually a milimetre or so square.
Of course, the 'flakes' need to be flat in order to view them properly. I thought I might be able to pipette them, in a drop of the media they're already in, onto nucleopore membranes and allow them to settle, then try to remove the rest of the media and hope that they stick through the dehydration process... but i'm not sure they will. I'm planning on using HMDS.
I'm goig to try it just to see what happens... but has anyone prepared a sample like this that could suggest something that is know to work well?
Cheers,
-- Scott J. Coutts --------------------------------------------------------------- Bacterial Pathogenesis Research Group Department of Microbiology, Box 53, Monash University, 3800, Australia Phone: 9905 4838 Fax: 9905 4811 ---------------------------------------------------------------
==============================Original Headers============================== 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005 8, 17 -- Received: from omta04sl.mx.bigpond.com (omta04sl.mx.bigpond.com [144.140.93.156]) 8, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6J6k1Km017389 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 01:46:02 -0500 8, 17 -- Received: from [60.224.29.18] by omta04sl.mx.bigpond.com with ESMTP 8, 17 -- id {20050719064600.GCWB28893.omta04sl.mx.bigpond.com-at-[60.224.29.18]} 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 06:46:00 +0000 8, 17 -- Message-ID: {42DCA1AB.5070904-at-med.monash.edu.au} 8, 17 -- Date: Tue, 19 Jul 2005 16:46:03 +1000 8, 17 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au} 8, 17 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 8, 17 -- X-Accept-Language: en-us, en 8, 17 -- MIME-Version: 1.0 8, 17 -- To: microscopy-at-microscopy.com 8, 17 -- Subject: Another SEM sample prep question 8, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Scott, Is there a specific reason for looking at these bacteria with SEM? cheers, Roseamry
Dr. Rosemary White rosemary.white-at-csiro.au Microscopy Centre ph. 61-2-6246 5475 CSIRO Plant Industry mob. 61-0402 835 973 GPO Box 1600 fax. 61-2-6246 5334 Canberra, ACT 2601 Australia
} From: scott.coutts-at-med.monash.edu.au } Reply-To: microscopy-at-microscopy.com } Date: Tue, 19 Jul 2005 01:47:57 -0500 } To: rosemary.white-at-csiro.au } Subject: [Microscopy] Another SEM sample prep question } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } } I need to prep some samples for SEM, but I'm not sure of the best way to } go about it. } } We have some bacteria that normally grow in a type of liquid nutrient } medium as free, discrete and seperate motile cells. However, we have } come across a strain that forms into 'mats' on the bottom of the culture } vessel. It is these that we would like to examine by SEM. } } The problem is that the 'mats' are not like a normal biofilm in that } they are not strongly adherant to a solid surface, and the mat is not } very robust. The mats will 'float' off the bottom of the culture vessel } if they are disturbed and they will fragment into small flakes if the } vessel is shaken or swirled. The fragments are usually a milimetre or so } square. } } Of course, the 'flakes' need to be flat in order to view them properly. } I thought I might be able to pipette them, in a drop of the media } they're already in, onto nucleopore membranes and allow them to settle, } then try to remove the rest of the media and hope that they stick } through the dehydration process... but i'm not sure they will. I'm } planning on using HMDS. } } I'm goig to try it just to see what happens... but has anyone prepared a } sample like this that could suggest something that is know to work well? } } Cheers, } } -- } Scott J. Coutts } --------------------------------------------------------------- } Bacterial Pathogenesis Research Group } Department of Microbiology, } Box 53, Monash University, 3800, Australia } Phone: 9905 4838 Fax: 9905 4811 } --------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005 } 8, 17 -- Received: from omta04sl.mx.bigpond.com (omta04sl.mx.bigpond.com } [144.140.93.156]) } 8, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6J6k1Km017389 } 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 01:46:02 -0500 } 8, 17 -- Received: from [60.224.29.18] by omta04sl.mx.bigpond.com with ESMTP } 8, 17 -- id } {20050719064600.GCWB28893.omta04sl.mx.bigpond.com-at-[60.224.29.18]} } 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 06:46:00 } +0000 } 8, 17 -- Message-ID: {42DCA1AB.5070904-at-med.monash.edu.au} } 8, 17 -- Date: Tue, 19 Jul 2005 16:46:03 +1000 } 8, 17 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au} } 8, 17 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) } 8, 17 -- X-Accept-Language: en-us, en } 8, 17 -- MIME-Version: 1.0 } 8, 17 -- To: microscopy-at-microscopy.com } 8, 17 -- Subject: Another SEM sample prep question } 8, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 17 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 23 -- From Rosemary.White-at-csiro.au Tue Jul 19 01:52:08 2005 4, 23 -- Received: from vic-ironport-ext-out2.csiro.au (vic-ironport-ext-out2.csiro.au [150.229.64.38]) 4, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6J6q7M5025152 4, 23 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 01:52:07 -0500 4, 23 -- Received: from exgw1-mel.nexus.csiro.au (138.194.3.56) 4, 23 -- by vic-ironport-ext-out2.csiro.au with ESMTP; 19 Jul 2005 16:52:05 +1000 4, 23 -- X-BrightmailFiltered: true 4, 23 -- X-Brightmail-Tracker: AAAAAQAAA+k= 4, 23 -- X-IronPort-AV: i="3.93,298,1114956000"; 4, 23 -- d="scan'208"; a="46134978:sNHT22338420" 4, 23 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 4, 23 -- Tue, 19 Jul 2005 16:52:04 +1000 4, 23 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 4, 23 -- Date: Tue, 19 Jul 2005 16:53:19 +1000 4, 23 -- Subject: Re: [Microscopy] Another SEM sample prep question 4, 23 -- From: Rosemary White {Rosemary.White-at-csiro.au} 4, 23 -- To: {microscopy-at-microscopy.com} 4, 23 -- Message-ID: {BF02E07F.10AE9%Rosemary.White-at-csiro.au} 4, 23 -- In-Reply-To: {200507190647.j6J6lvFi022584-at-ns.microscopy.com} 4, 23 -- Mime-version: 1.0 4, 23 -- Content-type: text/plain; charset="US-ASCII" 4, 23 -- Content-transfer-encoding: 7bit 4, 23 -- X-OriginalArrivalTime: 19 Jul 2005 06:52:04.0345 (UTC) FILETIME=[5F326690:01C58C2E] ==============================End of - Headers==============================
IMAQUE IMAGING INC CALL 571-437-6593 ----- Original Message ----- X-from: {luce-at-earthtech.org} To: {JSMIT51-at-TAMPABAY.RR.COM} Sent: Sunday, July 17, 2005 7:05 PM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Tuesday, July 19, 2005 at 04:02:00 ---------------------------------------------------------------------------
Email: K.venner-at-ion.ucl.ac.uk Name: kerrie
Organization: Institute of Neurology, University College London UK
Title-Subject: [Filtered] MListserver: digital ccd cameras versus digital plate system for TEM
Question: Many thanks to everyone who took the time and kindly contributed to the very long debate my query sparked up. All contributions have been compiled and have enabled me to present a balanced working view of the systems currently available.
there is a very simple way to look at bacteria in the SEM. just puch teh bacteria/liquid through a .45 micron filter. then process the filter paper for the SEM. this is a tried and true method. john
--- scott.coutts-at-med.monash.edu.au wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } } I need to prep some samples for SEM, but I'm not } sure of the best way to } go about it. } } We have some bacteria that normally grow in a type } of liquid nutrient } medium as free, discrete and seperate motile cells. } However, we have } come across a strain that forms into 'mats' on the } bottom of the culture } vessel. It is these that we would like to examine by } SEM. } } The problem is that the 'mats' are not like a normal } biofilm in that } they are not strongly adherant to a solid surface, } and the mat is not } very robust. The mats will 'float' off the bottom of } the culture vessel } if they are disturbed and they will fragment into } small flakes if the } vessel is shaken or swirled. The fragments are } usually a milimetre or so } square. } } Of course, the 'flakes' need to be flat in order to } view them properly. } I thought I might be able to pipette them, in a drop } of the media } they're already in, onto nucleopore membranes and } allow them to settle, } then try to remove the rest of the media and hope } that they stick } through the dehydration process... but i'm not sure } they will. I'm } planning on using HMDS. } } I'm goig to try it just to see what happens... but } has anyone prepared a } sample like this that could suggest something that } is know to work well? } } Cheers, } } -- } Scott J. Coutts } --------------------------------------------------------------- } Bacterial Pathogenesis Research Group } Department of Microbiology, } Box 53, Monash University, 3800, Australia } Phone: 9905 4838 Fax: 9905 4811 } --------------------------------------------------------------- } } ==============================Original } Headers============================== } 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul } 19 01:46:02 2005 } 8, 17 -- Received: from omta04sl.mx.bigpond.com } (omta04sl.mx.bigpond.com [144.140.93.156]) } 8, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j6J6k1Km017389 } 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 } Jul 2005 01:46:02 -0500 } 8, 17 -- Received: from [60.224.29.18] by } omta04sl.mx.bigpond.com with ESMTP } 8, 17 -- id } {20050719064600.GCWB28893.omta04sl.mx.bigpond.com-at-[60.224.29.18]} } 8, 17 -- for {microscopy-at-microscopy.com} ; } Tue, 19 Jul 2005 06:46:00 +0000 } 8, 17 -- Message-ID: } {42DCA1AB.5070904-at-med.monash.edu.au} } 8, 17 -- Date: Tue, 19 Jul 2005 16:46:03 +1000 } 8, 17 -- From: Scott Coutts } {scott.coutts-at-med.monash.edu.au} } 8, 17 -- User-Agent: Mozilla Thunderbird 1.0.2 } (Windows/20050317) } 8, 17 -- X-Accept-Language: en-us, en } 8, 17 -- MIME-Version: 1.0 } 8, 17 -- To: microscopy-at-microscopy.com } 8, 17 -- Subject: Another SEM sample prep question } 8, 17 -- Content-Type: text/plain; } charset=ISO-8859-1; format=flowed } 8, 17 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
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Interesting problem. The "matts" you refer to are likely to fragment or worse if you use the usual filter-based preparation methods . First question: do you have access to cryoSEM? If yes, this would be the best way to do your samples. Freeze in a high-pressure freezer, if available, or by plunging into slush nitrogen, then do the cryo SEM. This is the method most likely to give you the unaltered structure of the matts and the critters therein. If you don't have access to cryoSEM, then the best way is to use minifuge tubes or the like. Let the samples sit in the tube in fix, then the EtOH dehydration steps, being very careful when you withdraw the fluid to change it. Add the fluid for the next step as you withdraw the old fluid to minimize disturbance of the matts, and maybe add the fluid for each succeeding step down the side of the tube. I suggest a 2:1 1:1 1:2 EtOH:HMDS series before the 3 changes in 100% HMDS. I've found it helpful to deposit the last HMDS + sample drops on a sputter-coated membrane filter* stuck to a SEM stub for drying. This further minimizes handling. * 0.22 micron "nucleopore" type -- the ones with the nice, round holes, not the torturous-path type of filter. Sputter coat both sides of the filter before sticking to the stub, best, or stick to the stub with conductive carbon tabs, then sputter coat. Then drop on the samples in HMDS. (And yes, sputter coat for viewing in the SEM.)
Phil
} Hi All, } } I need to prep some samples for SEM, but I'm not sure of the best way to } go about it. } } We have some bacteria that normally grow in a type of liquid nutrient } medium as free, discrete and seperate motile cells. However, we have } come across a strain that forms into 'mats' on the bottom of the culture } vessel. It is these that we would like to examine by SEM. } } The problem is that the 'mats' are not like a normal biofilm in that } they are not strongly adherant to a solid surface, and the mat is not } very robust. The mats will 'float' off the bottom of the culture vessel } if they are disturbed and they will fragment into small flakes if the } vessel is shaken or swirled. The fragments are usually a milimetre or so } square. } } Of course, the 'flakes' need to be flat in order to view them properly. } I thought I might be able to pipette them, in a drop of the media } they're already in, onto nucleopore membranes and allow them to settle, } then try to remove the rest of the media and hope that they stick } through the dehydration process... but i'm not sure they will. I'm } planning on using HMDS. } } I'm goig to try it just to see what happens... but has anyone prepared a } sample like this that could suggest something that is know to work well? } } Cheers, } } -- } Scott J. Coutts } --------------------------------------------------------------- } Bacterial Pathogenesis Research Group } Department of Microbiology, } Box 53, Monash University, 3800, Australia } Phone: 9905 4838 Fax: 9905 4811 } --------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005 } 8, 17 -- Received: from omta04sl.mx.bigpond.com } (omta04sl.mx.bigpond.com [144.140.93.156]) } 8, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6J6k1Km017389 } 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 } 01:46:02 -0500 } 8, 17 -- Received: from [60.224.29.18] by omta04sl.mx.bigpond.com with ESMTP } 8, 17 -- id } {20050719064600.GCWB28893.omta04sl.mx.bigpond.com-at-[60.224.29.18]} } 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 } 06:46:00 +0000 } 8, 17 -- Message-ID: {42DCA1AB.5070904-at-med.monash.edu.au} } 8, 17 -- Date: Tue, 19 Jul 2005 16:46:03 +1000 } 8, 17 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au} } 8, 17 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) } 8, 17 -- X-Accept-Language: en-us, en } 8, 17 -- MIME-Version: 1.0 } 8, 17 -- To: microscopy-at-microscopy.com } 8, 17 -- Subject: Another SEM sample prep question } 8, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 17 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
-- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) http://www.ansci.wisc.edu/microscopy.htm
==============================Original Headers============================== 5, 27 -- From peoshel-at-wisc.edu Tue Jul 19 09:11:41 2005 5, 27 -- Received: from smtp7.wiscmail.wisc.edu (hagen.doit.wisc.edu [144.92.197.163]) 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6JEBeEu028672 5, 27 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 09:11:41 -0500 5, 27 -- Received: from avs-daemon.smtp7.wiscmail.wisc.edu by smtp7.wiscmail.wisc.edu 5, 27 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 5, 27 -- id {0IJV00B1UORFO1-at-smtp7.wiscmail.wisc.edu} for microscopy-at-microscopy.com; 5, 27 -- Tue, 19 Jul 2005 09:11:39 -0500 (CDT) 5, 27 -- Received: from [10.25.102.29] (ansci.wisc.edu [144.92.132.175]) 5, 27 -- by smtp7.wiscmail.wisc.edu 5, 27 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 5, 27 -- with ESMTPSA id {0IJV00MVUORDC9-at-smtp7.wiscmail.wisc.edu} for 5, 27 -- microscopy-at-microscopy.com; Tue, 19 Jul 2005 09:11:38 -0500 (CDT) 5, 27 -- Date: Tue, 19 Jul 2005 09:11:38 -0500 5, 27 -- From: Philip Oshel {peoshel-at-wisc.edu} 5, 27 -- Subject: Re: [Microscopy] Another SEM sample prep question 5, 27 -- In-reply-to: {200507190646.j6J6kc1d018856-at-ns.microscopy.com} 5, 27 -- X-Sender: peoshel-at-wiscmail.wisc.edu 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- Message-id: {p05210605bf02b64f46f3-at-[10.25.102.29]} 5, 27 -- MIME-version: 1.0 5, 27 -- Content-type: text/plain; format=flowed; charset=us-ascii 5, 27 -- Content-transfer-encoding: 7BIT 5, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.132.175 5, 27 -- X-Spam-PmxInfo: Server=avs-7, Version=4.7.1.128075, Antispam-Engine: 2.0.3.1, 5, 27 -- Antispam-Data: 2005.7.19.11, SenderIP=144.92.132.175 5, 27 -- References: {200507190646.j6J6kc1d018856-at-ns.microscopy.com} ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tantt-at-umich.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, July 19, 2005 at 09:03:46 ---------------------------------------------------------------------------
A few more details on what I wrote on this subject before. Lyson has been making inks and papers for photo quality ink jet printing for some time. Their new system is the "Daylight Darkroom". It is a dedicated 4 to 7 ink system (depending on your printer) for black and white printing only. I think the price includes software and inks but no printer, the system only works with certain printers. It has gotten very favorable reviews in fine art photo magazines (May/June 2005 issue of Photo Techniques, the review might be on the magazine's website (phototechmag.com) or on Lyson's). Lyson will send you some sample prints if you register at their website. I suspect that if someone sent Lyson a digital TEM or SEM image they might make a print of that as a sales tool. I'll bet they have no idea that there is a market for their products in the EM field. I only wish I did more than a few prints per year of parts of ground up cells. Lyson is not the only firm making inks, papers and printing systems for fine art B&W photography. Check out InkjetMall.com or MediaStreet.com. If you want to stick with an ink jet and the manufacturer's inks and papers the Canon i9900 is the Editor's Choice in the June 28 2005 issue of PC magazine. The same printer is also the first choice in Photo Techniques May/June 2005 issue. Someone on the list also liked this printer. If you like HP printers the PhotoSmart 8450 will take an HP 'photo gray' cartridge set for doing black and white prints. Disclaimer: I don't have any financial interest in any of the firms or products I mentioned.
Geoff
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 6, 29 -- From mcauliff-at-umdnj.edu Tue Jul 19 09:32:30 2005 6, 29 -- Received: from mail01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6JEWU4T011889 6, 29 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 19 Jul 2005 09:32:30 -0500 6, 29 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 6, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id A46DEEC0D0 6, 29 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 19 Jul 2005 10:32:29 -0400 (EDT) 6, 29 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 6, 29 -- by mail01.umdnj.edu (Proprietary) with ESMTP id F0CF1EC0C6 6, 29 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 19 Jul 2005 10:32:27 -0400 (EDT) 6, 29 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 6, 29 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 6, 29 -- id {0IJV00401P5RJY-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 6, 29 -- for Microscopy-at-msa.microscopy.com; Tue, 19 Jul 2005 10:32:27 -0400 (EDT) 6, 29 -- Received: from [127.0.0.1] ([10.138.2.240]) 6, 29 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 6, 29 -- 2004)) with ESMTP id {0IJV00IVXPOJ8M-at-Polaris.umdnj.edu} ; Tue, 6, 29 -- 19 Jul 2005 10:31:31 -0400 (EDT) 6, 29 -- Date: Tue, 19 Jul 2005 10:31:34 -0400 6, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 6, 29 -- Subject: dye sub, ink jet etc for prints 6, 29 -- To: Microscopy-at-msa.microscopy.com, "John J. Bozzola" {bozzola-at-siu.edu} 6, 29 -- Message-id: {42DD0EC6.90103-at-umdnj.edu} 6, 29 -- MIME-version: 1.0 6, 29 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 6, 29 -- Content-transfer-encoding: 7BIT 6, 29 -- X-Accept-Language: en-us, en 6, 29 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 6, 29 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
hoffpajo-at-yahoo.com wrote: } } there is a very simple way to look at bacteria in the } SEM. just puch teh bacteria/liquid through a .45 } micron filter. then process the filter paper for the } SEM. this is a tried and true method. } john }
Yes, but that doesnt help me to lay mats of bacteria onto a support - filtering them is what we normally do for the free-living cells, but if I filter the mats, firstly there's a good chance that they;ll get stuck in the syringe, and secondly, they won't come out flat on the filter. THey'll be all bunched up or folded.
-- Scott J. Coutts --------------------------------------------------------------- Bacterial Pathogenesis Research Group Department of Microbiology, Box 53, Monash University, 3800, Australia Phone: 9905 4838 Fax: 9905 4811 ---------------------------------------------------------------
==============================Original Headers============================== 3, 19 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 09:53:59 2005 3, 19 -- Received: from omta05ps.mx.bigpond.com (omta05ps.mx.bigpond.com [144.140.83.195]) 3, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6JErwbY019859 3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 09:53:59 -0500 3, 19 -- Received: from [60.224.29.18] by omta05ps.mx.bigpond.com with ESMTP 3, 19 -- id {20050719145357.UTTR21584.omta05ps.mx.bigpond.com-at-[60.224.29.18]} 3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 14:53:57 +0000 3, 19 -- Message-ID: {42DD1404.3020406-at-med.monash.edu.au} 3, 19 -- Date: Wed, 20 Jul 2005 00:53:56 +1000 3, 19 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au} 3, 19 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 3, 19 -- X-Accept-Language: en-us, en 3, 19 -- MIME-Version: 1.0 3, 19 -- To: microscopy-at-microscopy.com 3, 19 -- Subject: Re: [Microscopy] Re: Another SEM sample prep question 3, 19 -- References: {200507191344.j6JDi0Zi028149-at-ns.microscopy.com} 3, 19 -- In-Reply-To: {200507191344.j6JDi0Zi028149-at-ns.microscopy.com} 3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This is interesting information. Would you be willing to write it up for MIcroscopy Today? With illustrations and all. We are interested in running such an article, and there would be room for the details and so forth that you can't put in an email.
Phil (also still not entirely retired from Tech Ed. at Microscopy Today)
} Thanks everyone, I've gotten lots of helpful advice and comments, so } I'll try to sum up my conclusions. } } Some background: Silicon carbide (SiC) is produced by a very old } company Washington Mills located near Niagara Falls but production is } in Illinois. Blessed folks; they sent me a sample of crystals for free. } } {http://www.medibix.com/company.jsp?company_id=10001791} } } It is made in tonnage quantities as clusters of glossy flat hexagonal } black crystals up to a few centimeters across, and which are actually } deep transparent blue and are crushed for abrasives and metallurgy. } The cost of this industrial crystalline material is negligible. The } Cree Corporation in NC makes also clear white SiC crystals and wafers } for a fairly high price, but only sells them already faceted into } clear diamond substitutes for jewelry. Such clear SiC crystals are } termed "moissenite". } } SiC crystals are very hard and brittle and the edge breaks when you } try to sharpen them into knives using anything resembling normal gem } faceting methods. But knives can be made using the proper lapping } technique and the appropriate grades of diamond powder. Here is an } interesting link on modern diamond powder technology: } } {http://www.ceramicindustry.com/CDA/ArticleInformation/features/ } BNP__Features__Item/0,2710,152388,00.html} } } At any rate, besides glass, only hard single crystals of diamond, } sapphire, and silicon carbide seem to be appropriate for making } ultramicrotome knives, which by their nature demand a perfectly } homogeneous material. Sapphire knives were sold at one time by } Diatome but the price was reputedly about $500 apiece and they became } dull, unlike diamond knives, so the economics was questionable. } } My SiC knives become dull after a few hundred slices, probably } depending on the the hardness of the embedding plastic (glycol } methacrylate works well), but the raw materials are inexpensive and } my lapping machine only cost about $50 to build, so the labor of } making them is inherently the major cost. } } Since SiC crystals are difficult to sharpen without breaking the } edge, I assume the exact same lapping techniques would also be } appropriate for sapphire, which is softer tougher and less brittle } than SiC; the latter are the very devil to sharpen without breaking. } I learned how to make them through sheer stubbornness in order to } make many serial sections with my Porter Blum MT-2 without paying } $1000+ for a diamond knife. } } I can only guess at the durability of sapphire knives, but the fact } that they were once offered indicates that they might still be a } viable alternative for some applications if they were only available } at a lower cost. } } My SiC crystals are epoxyed in a slot cut in the apex of a steel } holder the same size and shape as a right angle glass knife. The } clean crystals are hydrophilic (I assume the exposed surface layer at } the atomic level is silicon oxide). I clean them by wiping the edge } sideways with Teflon and don't have many wetting problems. } } My conclusion is that I don't much want to go the trouble of trying } to make money patenting, licensing and selling knives, but that I } should publish my technique of making them, most likely in the Review } of Scientific Instruments, and see if the world pays attention. I } don't think the method of sharpening hard crystals to give very sharp } edges has ever been published; I can't find it in the scientific } literature. I seriously doubt it would work for diamonds without } modification. } } The only possible drawback to open publication is that no one maker } could make a lot of money making them, and thus might not offer them, } assuming they prove to be a modestly useful alternative to diamonds. } } I assume that maybe the Chinese could sell disposable knives for $20 } or so and they might catch on for student use and low budget and } occasional use. Glass knives are clearly sharper for a few dozen } sections and diamond knives clearly last longer. } } -- Roger Baker Jr. } 1303 Bentwood, } Austin Tx, 78722 } } } } } } } } } } } } } ==============================Original Headers============================== } 29, 19 -- From rcbaker-at-eden.infohwy.com Mon Jul 18 21:18:00 2005 } 29, 19 -- Received: from mx3.lsn.net (mx3.lsn.net [66.90.130.75]) } 29, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6J2Hw75015970 } 29, 19 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 } 21:17:59 -0500 } 29, 19 -- Received: from [192.168.1.100] } (66-90-146-134.dyn.grandenetworks.net [66.90.146.134]) } 29, 19 -- by mx3.lsn.net (8.13.0.Beta3/8.12.8) with ESMTP id } j6J2HkKD013027 } 29, 19 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 } 21:17:50 -0500 } 29, 19 -- Mime-Version: 1.0 (Apple Message framework v733) } 29, 19 -- In-Reply-To: {200507182248.j6IMmje4011361-at-ns.microscopy.com} } 29, 19 -- References: {200507182248.j6IMmje4011361-at-ns.microscopy.com} } 29, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 29, 19 -- Message-Id: {0FA04694-AC3E-4D7F-894D-13AD154CAF36-at-eden.infohwy.com} } 29, 19 -- Content-Transfer-Encoding: 7bit } 29, 19 -- From: Roger Baker {rcbaker-at-eden.infohwy.com} } 29, 19 -- Subject: Re: [Microscopy] Alternatives to diamond knives? } 29, 19 -- Date: Mon, 18 Jul 2005 21:17:44 -0500 } 29, 19 -- To: microscopy-at-microscopy.com } 29, 19 -- X-Mailer: Apple Mail (2.733) } 29, 19 -- X-Antivirus: Scanned by Vexira Antivirus 1.0.6 } ==============================End of - Headers==============================
-- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) http://www.ansci.wisc.edu/microscopy.htm
==============================Original Headers============================== 5, 27 -- From peoshel-at-wisc.edu Tue Jul 19 10:19:15 2005 5, 27 -- Received: from smtp7.wiscmail.wisc.edu (hagen.doit.wisc.edu [144.92.197.163]) 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6JFJFLi027873 5, 27 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 10:19:15 -0500 5, 27 -- Received: from avs-daemon.smtp7.wiscmail.wisc.edu by smtp7.wiscmail.wisc.edu 5, 27 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 5, 27 -- id {0IJV00F0QRVGWH-at-smtp7.wiscmail.wisc.edu} for microscopy-at-microscopy.com; 5, 27 -- Tue, 19 Jul 2005 10:18:52 -0500 (CDT) 5, 27 -- Received: from [10.25.102.29] (ansci.wisc.edu [144.92.132.175]) 5, 27 -- by smtp7.wiscmail.wisc.edu 5, 27 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 5, 27 -- with ESMTPSA id {0IJV00G1BRVB3H-at-smtp7.wiscmail.wisc.edu} for 5, 27 -- microscopy-at-microscopy.com; Tue, 19 Jul 2005 10:18:48 -0500 (CDT) 5, 27 -- Date: Tue, 19 Jul 2005 10:18:49 -0500 5, 27 -- From: Philip Oshel {peoshel-at-wisc.edu} 5, 27 -- Subject: [Microscopy] Re: Alternatives to diamond knives? 5, 27 -- In-reply-to: {200507190219.j6J2JWRe017424-at-ns.microscopy.com} 5, 27 -- X-Sender: peoshel-at-wiscmail.wisc.edu 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- Message-id: {p05210609bf02c9c1d87a-at-[10.25.102.29]} 5, 27 -- MIME-version: 1.0 5, 27 -- Content-type: text/plain; format=flowed; charset=us-ascii 5, 27 -- Content-transfer-encoding: 7BIT 5, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.132.175 5, 27 -- X-Spam-PmxInfo: Server=avs-3, Version=4.7.1.128075, Antispam-Engine: 2.0.3.1, 5, 27 -- Antispam-Data: 2005.7.19.14, SenderIP=144.92.132.175 5, 27 -- References: {200507190219.j6J2JWRe017424-at-ns.microscopy.com} ==============================End of - Headers==============================
I second Sousan's reply. It saves downloading a lot of different emails! I also found the information quite helpful.
Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Monday, July 18, 2005 5:22 PM To: Sherwood, Margaret
Dear Listers,
Below is a compilation of the replies I received to my recent question about Cu vs. Ni grids for immunolabeling. I usually don't do this without express permission, but most everybody posted to the list anyway, and I made sure to edit those that didn't. If it bothers anyone, let me know and I will never do it again.
In summary, it appears that copper can and will react with salts in buffers and other solutions, although some people still have decent luck. This problem can be ameliorated by using coated Cu grids and only wetting the coated side and by shortening incubation times
Also, Marc Pypaert had the interesting suggestion of just using Ni grids for everything, since they're not much more expensive, thereby avoiding the problem entirely. Any thoughts on this?
Many thanks to everyone who replied! I now have a much better response to our clients' questions on this matter (not to mention my own).
Cheers, Randy
Over the years I have frequently seen cases where the samples themselves have reacted with the copper grid. Two examples:
1) Looking at Fe-S precipitates in magnetotactic bacteria, when the precipitates were ugly and analyzed as copper sulphide. Our surmise was that the iron and copper had undergone ion exchange while the grid (and carbon film) was wet with the culture medium. When we used nickel grids, the precipitates were well formed and composed of iron and sulphur.
2) Looking at Si precipitates in Al-Si electronic bond wire. The specimens were prepared by embedding and microtoming, floating in DI water. The Si precipitates were surrounded by an ugly mess containing masses of copper. Again, presumed to me electrochemical reaction between the Al and the Cu grid, through the DI water medium, somehow preferentially at the Si precipitates. Again, use of Ni grids produced good pictures allowing us to characterize the precipitation.
Having said all that, we continue to default to use copper grids!
Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
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The main problem encountered with copper grids used for immunocytochemistry or immunogold labeling is the reaction of the copper with salts in the buffers used during labeling. This is a time-related reaction, and can usually be avoided by having short labeling runs and using grids with films on them. Gilder grids, available from major microscope supply vendors, are gold-coated copper grids, and are not that much more expensive than regular copper grids. These grids may be the variety used by researchers who have reported having no problems with their grids during their immuno runs. I usually use nickel grids for my work, but have used formvar-coated copper grids without problems for 1 day immuno runs so long as the film is intact and as long as I don't get clumsy and end up sinking my grids in my solutions (if I do sink them, I do a quick distilled water rinse, blot the back of the grids with filter paper until almost dry, then re-float them where I left off). As others have shared, the nickel grids are much sturdier, not too expensive, and are not a problem to handle as long as you use antimagnetic forceps. With the nickel grids, remember to correct for astigmatism in the TEM by using a hole in your sample on the nickel grid. This will accommodate for any inherent residual magnetic field in the grid itself.
Edward Haller Lab Manager, Diagnostic Electron Microscopy Lab University of South Florida Pathology Department Tampa, FL 33612
We have had problems with Cu grids, even Formvar coated ones. I have precoated copper grids with Parlodion by dipping, blotting and drying before use, with or with out a formvar coating. THat method seems to protect the copper from reacting with PBS or whatever. This is not original to me, but I cannot recall where I read this (among many other things I can no longer recall). I think it might have been one of those smart Swiss guys.
Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 -------------------------------------------------------------------
I had a bad experience with formvar-copper grids in immunogold experiment many years ago when incubating primary antibody overnight and the rest done on the following morning. I am using Nickel grids now -- no more problems.
Ann Fook Yang EM Unit/ Unite EM Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada 960 Carling Ave/960 Boul Carling Ottawa,Ontario/Ottawa, Ontario Canada K1A 0C6 ------------------------------------------------------------------------ ------------
recently i did a long ultracentrifuge run to load some 10S particles onto a formvar-copper grid. the grids reacted with the phosphate buffer over the 4 hour spin time. not a pretty picture - i had to go back to my collaborator and get fresh material so we could put it onto formvar-nickel grids. wonderful gentleman.
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 ----------------------------------------------------------------
We used Cu grids for many years. Normally we use formvar + carbon coated grids for ICC since we have a lot less loss of sections. We also tend to do long incubations ...usually overnight. This is time efficient and also lets us use highly diluted antibody so also minimize background due to cross-reactions from contaminants in polyclonal antibodies.
Occasionally we would have a reaction due to copper oxidizing with resultant green solution. I attributed this to salts or traces of Tween 20 in the buffer and breaks in the coating exposing the Cu. As far as I can see, this is the only reason for not using Cu grids if you use coated grids. We have switched for the most part to using coated Ni grids to avoid this problem.
On the other hand, occasionally we need to do a double labeling using uncoated grids and both sides of the sections. I would hesitate to use Cu for this and prefer Ni. Gold would work but is both more expensive and more delicate than Ni grids.
Debby Sherman, Manager Life Science Microscopy Facility Purdue University S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 ------------------------------------------------------------------------ -
X-from my experience, coated copper grids will be fine for most (if not all) immunolabelling, providing the solutions do not wet the uncoated side of the grid. Uncoated copper grids always have been fine when I've used PBS.
However, copper grids (uncoated - or the uncoated side) usually will react with Tris-HCl buffers and some of the high salt buffer formulations that I've used. Longer incubation times (eg. overnight) cause more reaction with the grid than shorter times (eg. 60mins) - so you may be able to use copper grids without problems if incubations are short, even with buffer formulations that might react with the grid if left for longer periods. It also is more difficult to prevent wetting of the reverse (uncoated) side of coated copper grids if incubation times are long, and particularly if wetting agents are used in the buffer formulation.
When I started immunolabelling in the early 1980s - and hadn't yet heard about the use of nickel grids - I used uncoated copper grids (and PBS) for years without problems. My current lab generally uses a high salt Tris-HCl buffer which includes a small amount of Tween 20, so we routinely use nickel grids for immunolabelling. Nickel grids do have some disadvantages (eg. charge), but we run a multi-user facility, often training students or other inexperienced users, we've found nickel the best option to overcome potential "grid-reaction" problems. However, nickel grids certainly aren't essential for successful immunolabelling - you just need to be aware of the possible problems.
Dr Deborah Stenzel Analytical Electron Microscopy Facility Queensland University of Technology GPO Box 2434 Brisbane 4001 Australia
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I know (from sad experience) that Tris buffers will etch Cu grids - the antibody droplets turn a lovely blue and there is gunk all over the sections. I use Ni grids, only because I prefer Tris buffers (no real reason why, I guess) for IEM and my current PI cringes at buying Au grids. I know one person who does all of her labelling in phosphate buffer and uses Cu grids with no precipitate problems.
As for the other reasons you Googled....I've heard them, but never seen any documentation nor had personal experience.
Can't wait to hear if anyone has actual evidence for some of the other no-Cu reasons!
Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 ----------------------------------------------------------------
Hi Randy, I had problems with Cu grids when incubating sections for long periods - overnight for instance. The copper would react with whatever was available and form green-blue salts. Not unexpected. We switched to nickel grids for a while but they charge, gold but they are delicate...by this time we had shortened the incubation times used, and tried copper again... And had no problem using coated grids and incubation times of an hour or so. Grids are floated on drops of media so that only the coated side is wetted - I don't know if that is important.
Dr SJ Stowe Facility Coordinator ANU Electron Microscopy Unit ANU CRICOS#00120C
I only ran into the "use only Ni or Au grids" rule here, in my current job, and my predecessor certainly produced some superb images showing gold labelling of TEM sections. Blissfully unaware of this rule, I had been using copper grids for all EM immunolabelling. To get good labelling of one particular structure, which is about 40 nm diameter, I used uncoated thin-bar Cu grids so I could get labelling on both sides of the section - seemed to work just fine. I'll be interested to see the responses to your question.
Dr. Rosemary White Microscopy Centre CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 54, 25 -- From TindallR-at-missouri.edu Mon Jul 18 16:21:32 2005 54, 25 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 54, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6ILLW7R028963 54, 25 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 16:21:32 -0500 54, 25 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 54, 25 -- Mon, 18 Jul 2005 16:21:31 -0500 54, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 54, 25 -- Content-class: urn:content-classes:message 54, 25 -- MIME-Version: 1.0 54, 25 -- Content-Type: text/plain; 54, 25 -- charset="us-ascii" 54, 25 -- Subject: TEM: Immunocytochemistry and Cu grids 54, 25 -- Date: Mon, 18 Jul 2005 16:21:30 -0500 54, 25 -- Message-ID: {BA876152E8653240BE8572E897083EE79802FD-at-UM-EMAIL09.um.umsystem.edu} 54, 25 -- X-MS-Has-Attach: 54, 25 -- X-MS-TNEF-Correlator: 54, 25 -- Thread-Topic: TEM: Immunocytochemistry and Cu grids 54, 25 -- Thread-Index: AcWL3qpvaFt7UpRlRY2LVYYS/0TUZg== 54, 25 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 54, 25 -- To: {microscopy-at-microscopy.com} 54, 25 -- Cc: "Jensen, Cheryl A." {JensenC-at-missouri.edu} , 54, 25 -- "Chance, Deborah L." {ChanceD-at-health.missouri.edu} 54, 25 -- X-OriginalArrivalTime: 18 Jul 2005 21:21:31.0576 (UTC) FILETIME=[AAE06380:01C58BDE] 54, 25 -- Content-Transfer-Encoding: 8bit 54, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6ILLW7R028963 ==============================End of - Headers==============================
==============================Original Headers============================== 66, 23 -- From MSHERWOOD-at-PARTNERS.ORG Tue Jul 19 10:20:43 2005 66, 23 -- Received: from PHSXCON5.partners.org (phsxcon5.mgh.harvard.edu [132.183.130.38]) 66, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6JFKgkt030890 66, 23 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 10:20:43 -0500 66, 23 -- Received: from PHSXMB1.partners.org ([132.183.118.117]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.211); 66, 23 -- Tue, 19 Jul 2005 11:20:42 -0400 66, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 66, 23 -- Content-class: urn:content-classes:message 66, 23 -- MIME-Version: 1.0 66, 23 -- Content-Type: text/plain; 66, 23 -- charset="iso-8859-1" 66, 23 -- Subject: RE: [Microscopy] TEM: Immunocytochemistry and Cu grids 66, 23 -- Date: Tue, 19 Jul 2005 11:20:42 -0400 66, 23 -- Message-ID: {1AF23D0AD12E7444A5DB083CA978B73403F9258E-at-PHSXMB1.partners.org} 66, 23 -- X-MS-Has-Attach: 66, 23 -- X-MS-TNEF-Correlator: 66, 23 -- Thread-Topic: [Microscopy] TEM: Immunocytochemistry and Cu grids 66, 23 -- Thread-Index: AcWL3sjn9bEx0u/LQYmcg5JsgJpv5gAloloA 66, 23 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 66, 23 -- To: {microscopy-at-microscopy.com} 66, 23 -- X-OriginalArrivalTime: 19 Jul 2005 15:20:42.0372 (UTC) FILETIME=[6D5BC440:01C58C75] 66, 23 -- Content-Transfer-Encoding: 8bit 66, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6JFKgkt030890 ==============================End of - Headers==============================
This sounds like a job for ESEM. How much magnification do you need? You can work with fully hydrated samples. You can't get into the ultrastructual range of mags but excellent imaging in the classic SEM mag range, particularly with the newer line of ESEM's.
Tobias } } } hoffpajo-at-yahoo.com wrote: } } } } there is a very simple way to look at bacteria in the } } SEM. just puch teh bacteria/liquid through a .45 } } micron filter. then process the filter paper for the } } SEM. this is a tried and true method. } } john } } } } Yes, but that doesnt help me to lay mats of bacteria onto a support - } filtering them is what we normally do for the free-living cells, but if } I filter the mats, firstly there's a good chance that they;ll get stuck } in the syringe, and secondly, they won't come out flat on the filter. } THey'll be all bunched up or folded. } } -- } Scott J. Coutts } --------------------------------------------------------------- } Bacterial Pathogenesis Research Group } Department of Microbiology, } Box 53, Monash University, 3800, Australia } Phone: 9905 4838 Fax: 9905 4811 } --------------------------------------------------------------- } }
Hi, How about using a wire loop to place a mat of cells on a poly-L-lysine coated cover slip and then running it through fix, dehydration, etc.... Maybe you don't need the loop at all. Just slide the cover slip under a mat and lift out of the solution. I've never done a mat of cells but I've had success with SEM of cells from suspension on coated cover slips or pieces of Fisher Brand Plus Slides.
Andy Carol UIC Biology Chicago, IL
On Tue, July 19, 2005 9:54 am, scott.coutts-at-med.monash.edu.au said: } } } } ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ---------------------------------------------------------------------------- } } hoffpajo-at-yahoo.com wrote: } } there is a very simple way to look at bacteria in the } } SEM. just puch teh bacteria/liquid through a .45 } } micron filter. then process the filter paper for the } } SEM. this is a tried and true method. } } john } } Yes, but that doesnt help me to lay mats of bacteria onto a support - filtering them is what we normally do for the free-living cells, but if I filter the mats, firstly there's a good chance that they;ll get stuck in the syringe, and secondly, they won't come out flat on the filter. THey'll be all bunched up or folded. } } -- } Scott J. Coutts } --------------------------------------------------------------- } Bacterial Pathogenesis Research Group } Department of Microbiology, } Box 53, Monash University, 3800, Australia } Phone: 9905 4838 Fax: 9905 4811 } --------------------------------------------------------------- } } ==============================Original } Headers============================== } 3, 19 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 09:53:59 2005 3, 19 -- Received: from omta05ps.mx.bigpond.com (omta05ps.mx.bigpond.com [144.140.83.195]) } 3, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6JErwbY019859 } 3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 09:53:59 -0500 } 3, 19 -- Received: from [60.224.29.18] by omta05ps.mx.bigpond.com with ESMTP } 3, 19 -- id } {20050719145357.UTTR21584.omta05ps.mx.bigpond.com-at-[60.224.29.18]} 3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 14:53:57 +0000 } 3, 19 -- Message-ID: {42DD1404.3020406-at-med.monash.edu.au} } 3, 19 -- Date: Wed, 20 Jul 2005 00:53:56 +
You can find them at: http://www.precisiontem.com/ttools.html
If you are looking for tools for precision cross sectioning, you may also want to visit our website and look at the the following products:
Model 590 Tripod Polisher® Model 595 BiPod Polisher™
The Model 590 Tripod Polisher® is based on the original design by IBM East Fishkill and is used for SEM, TEM cross sectioning. The Model 595 BiPod™ Polisher is more similar to the T-tool although it incorporates many of the unique and critical features of the original Tripod Polisher® as well.
Please feel free to contact me off-line for details and pricing.
Best regards-
David
tantt-at-umich.edu wrote:
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Unless you can get life from the knife that is comparable to diamond or cost comparable to glass. The cost of labor make any thing much more expensive than glass a very hard sell when a diamond knife cost less than a week of a lab tech time not including the work lost while hie was messing around with knives when he could be doing more productive work.
Gordon Couger Stillwater, OK www.couger.com/gcouger
rcbaker-at-eden.infohwy.com wrote: } } On Jul 18, 2005, at 5:48 PM, sryazant-at-ucla.edu wrote: } } } Dear Roger } } I am not sure is tungsten carbide is similar to SiC or not. My } } experience } } with tungsten carbide basically was negative - this knife starts } } scratching } } the surface after just 50+ 0.5 um sections of standard plastic } } embedded } } tissue (no hard inclusions etc). As manufacturer explained to me it's } } because of polycrystalline nature of the material - small crystals } } just } } became loose and left the edge creating ruff surface. From this } } point of } } view, amorphous (like glass) or monocrystal (like diamond) material is } } preferable for good sections. Sapphire (hardness is 9) knifes were } } on the } } market for while without great success. From economical point of } } view, } } tungsten carbide knifes were also not so good - $100 each with } } ability to } } produce only 50+ good sections. If SiC acts in the similar way, } } then it } } would be difficult to use it in EM applications. Another aspect of } } using } } exotic materials - is it hydrophilic or hydrophobic? Could you use } } water } } with them? How easy to clean it up and handle? Sergey } } } } } Thanks everyone, I've gotten lots of helpful advice and comments, so } I'll try to sum up my conclusions. } } Some background: Silicon carbide (SiC) is produced by a very old } company Washington Mills located near Niagara Falls but production is } in Illinois. Blessed folks; they sent me a sample of crystals for free. } } {http://www.medibix.com/company.jsp?company_id=10001791} } } It is made in tonnage quantities as clusters of glossy flat hexagonal } black crystals up to a few centimeters across, and which are actually } deep transparent blue and are crushed for abrasives and metallurgy. } The cost of this industrial crystalline material is negligible. The } Cree Corporation in NC makes also clear white SiC crystals and wafers } for a fairly high price, but only sells them already faceted into } clear diamond substitutes for jewelry. Such clear SiC crystals are } termed "moissenite". } } SiC crystals are very hard and brittle and the edge breaks when you } try to sharpen them into knives using anything resembling normal gem } faceting methods. But knives can be made using the proper lapping } technique and the appropriate grades of diamond powder. Here is an } interesting link on modern diamond powder technology: } } {http://www.ceramicindustry.com/CDA/ArticleInformation/features/ } BNP__Features__Item/0,2710,152388,00.html} } } At any rate, besides glass, only hard single crystals of diamond, } sapphire, and silicon carbide seem to be appropriate for making } ultramicrotome knives, which by their nature demand a perfectly } homogeneous material. Sapphire knives were sold at one time by } Diatome but the price was reputedly about $500 apiece and they became } dull, unlike diamond knives, so the economics was questionable. } } My SiC knives become dull after a few hundred slices, probably } depending on the the hardness of the embedding plastic (glycol } methacrylate works well), but the raw materials are inexpensive and } my lapping machine only cost about $50 to build, so the labor of } making them is inherently the major cost. } } Since SiC crystals are difficult to sharpen without breaking the } edge, I assume the exact same lapping techniques would also be } appropriate for sapphire, which is softer tougher and less brittle } than SiC; the latter are the very devil to sharpen without breaking. } I learned how to make them through sheer stubbornness in order to } make many serial sections with my Porter Blum MT-2 without paying } $1000+ for a diamond knife. } } I can only guess at the durability of sapphire knives, but the fact } that they were once offered indicates that they might still be a } viable alternative for some applications if they were only available } at a lower cost. } } My SiC crystals are epoxyed in a slot cut in the apex of a steel } holder the same size and shape as a right angle glass knife. The } clean crystals are hydrophilic (I assume the exposed surface layer at } the atomic level is silicon oxide). I clean them by wiping the edge } sideways with Teflon and don't have many wetting problems. } } My conclusion is that I don't much want to go the trouble of trying } to make money patenting, licensing and selling knives, but that I } should publish my technique of making them, most likely in the Review } of Scientific Instruments, and see if the world pays attention. I } don't think the method of sharpening hard crystals to give very sharp } edges has ever been published; I can't find it in the scientific } literature. I seriously doubt it would work for diamonds without } modification. } } The only possible drawback to open publication is that no one maker } could make a lot of money making them, and thus might not offer them, } assuming they prove to be a modestly useful alternative to diamonds. } } I assume that maybe the Chinese could sell disposable knives for $20 } or so and they might catch on for student use and low budget and } occasional use. Glass knives are clearly sharper for a few dozen } sections and diamond knives clearly last longer. } } -- Roger Baker Jr. } 1303 Bentwood, } Austin Tx, 78722 } } } } } } } } } } } } } ==============================Original Headers============================== } 29, 19 -- From rcbaker-at-eden.infohwy.com Mon Jul 18 21:18:00 2005 } 29, 19 -- Received: from mx3.lsn.net (mx3.lsn.net [66.90.130.75]) } 29, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6J2Hw75015970 } 29, 19 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 21:17:59 -0500 } 29, 19 -- Received: from [192.168.1.100] (66-90-146-134.dyn.grandenetworks.net [66.90.146.134]) } 29, 19 -- by mx3.lsn.net (8.13.0.Beta3/8.12.8) with ESMTP id j6J2HkKD013027 } 29, 19 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2005 21:17:50 -0500 } 29, 19 -- Mime-Version: 1.0 (Apple Message framework v733) } 29, 19 -- In-Reply-To: {200507182248.j6IMmje4011361-at-ns.microscopy.com} } 29, 19 -- References: {200507182248.j6IMmje4011361-at-ns.microscopy.com} } 29, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 29, 19 -- Message-Id: {0FA04694-AC3E-4D7F-894D-13AD154CAF36-at-eden.infohwy.com} } 29, 19 -- Content-Transfer-Encoding: 7bit } 29, 19 -- From: Roger Baker {rcbaker-at-eden.infohwy.com} } 29, 19 -- Subject: Re: [Microscopy] Alternatives to diamond knives? } 29, 19 -- Date: Mon, 18 Jul 2005 21:17:44 -0500 } 29, 19 -- To: microscopy-at-microscopy.com } 29, 19 -- X-Mailer: Apple Mail (2.733) } 29, 19 -- X-Antivirus: Scanned by Vexira Antivirus 1.0.6 } ==============================End of - Headers============================== } }
==============================Original Headers============================== 4, 20 -- From gcc-at-couger.com Tue Jul 19 13:43:33 2005 4, 20 -- Received: from lakermmtai10.cox.net (lakermmtai10.cox.net [68.230.240.49]) 4, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6JIhWKX004223 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 13:43:33 -0500 4, 20 -- Received: from [127.0.0.1] (really [68.12.43.168]) by lakermmtao04.cox.net 4, 20 -- (InterMail vM.6.01.04.00 201-2131-118-20041027) with ESMTP 4, 20 -- id {20050719074647.GYID21705.lakermmtao04.cox.net-at-[127.0.0.1]} 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 03:46:47 -0400 4, 20 -- Message-ID: {42DCAFF3.6000703-at-couger.com} 4, 20 -- Date: Tue, 19 Jul 2005 02:46:59 -0500 4, 20 -- From: Gordon Couger {gcc-at-couger.com} 4, 20 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 4, 20 -- X-Accept-Language: en-us, en 4, 20 -- MIME-Version: 1.0 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- Subject: Re: [Microscopy] Re: Alternatives to diamond knives? 4, 20 -- References: {200507190222.j6J2MSVS021000-at-ns.microscopy.com} 4, 20 -- In-Reply-To: {200507190222.j6J2MSVS021000-at-ns.microscopy.com} 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Center for Materials Science and Engineering at the Massachusetts Institute of Technology has an immediate vacancy for a research specialist in electron microscopy. A description of the vacancy, together with MIT's employment policies, benefits, and other information, with links for on-line application is available at the following URL: http://sh.webhire.com/servlet/av/jd?ai=631&ji=1620589&sn=I or applications may be forwarded by electronic or paper mail to Ms. Jennifer Crockett, CMSE Assistant Director, Room # 13-2106, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307, e-mail crockett-at-mit.edu.
The application review process will be open until a qualified candidate has been identified. Questions about this position may be addressed to the undersigned.
Tony Garratt-Reed
*********************************************** Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
I used to work with Lyson "grey" inks and paper. It was previous generation, not "Daylight Darkroom" line. The quality of images was (yes, was) outstanding - no comparison to laser or dye-sub. The problem appeared to happens month later - the images faded very quickly: in month they lost about 50% of tone. I was trying different type of paper - no luck. Right now I am trying "grey" inks from different vendor, so I'll see. As far as I understand from numerous conversation with people from graphic design and digital photography area, it's practically impossible to protect ink-jet images from fading if stored not in album with protective cover. Another thing: glossy paper always bad in terms of fading. Matte paper is the best. Pigment inks are better than organic, but tends to clog the printer's head. After HP patent on ink-jet technology expired, Epson jumped into the market with few serious enhancements of the HP technology. I think they used piezo-element to disperse the inks and probably some other tricks. I am very happy with Epson 890 model. It was quite expensive a few years ago. You could get it for 10$ at eBay now. It has 6 inks and quality of color (with Epson cartridges) and B&W prints just outstanding. It's quite slow and as I mentioned above B&W images with Lyson inks tends to fade quick. No interest in Epson, just happy user. Sergey
At 07:34 AM 7/19/2005, you wrote:
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==============================Original Headers============================== 6, 25 -- From sryazant-at-ucla.edu Tue Jul 19 16:01:12 2005 6, 25 -- Received: from smtp-7.smtp.ucla.edu (smtp-7.smtp.ucla.edu [169.232.46.137]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6JL1B64021637 6, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 16:01:12 -0500 6, 25 -- Received: from mail.ucla.edu (mail.ucla.edu [169.232.48.141]) 6, 25 -- by smtp-7.smtp.ucla.edu (8.13.4/8.13.4) with ESMTP id j6JL19Lw020638 6, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 14:01:09 -0700 6, 25 -- Received: from kopoba.ucla.edu (ts12-160.dialup.bol.ucla.edu [169.232.225.107]) 6, 25 -- (authenticated bits=0) 6, 25 -- by mail.ucla.edu (8.13.4/8.13.4) with ESMTP id j6JL0x9H027334 6, 25 -- (version=TLSv1/SSLv3 cipher=DES-CBC3-SHA bits=168 verify=NOT) 6, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 14:01:03 -0700 6, 25 -- Message-Id: {6.1.2.0.2.20050719134117.027cf908-at-mail.ucla.edu} 6, 25 -- X-Sender: sryazant-at-mail.ucla.edu 6, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 25 -- Date: Tue, 19 Jul 2005 14:00:49 -0700 6, 25 -- To: microscopy-at-microscopy.com 6, 25 -- From: Sergey {sryazant-at-ucla.edu} 6, 25 -- Subject: Re: [Microscopy] dye sub, ink jet etc for prints 6, 25 -- In-Reply-To: {200507191434.j6JEYMqH014111-at-ns.microscopy.com} 6, 25 -- References: {200507191434.j6JEYMqH014111-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 25 -- X-Probable-Spam: no 6, 25 -- X-Scanned-By: smtp.ucla.edu on 169.232.46.137 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Tuesday, July 19, 2005 at 10:11:07 ---------------------------------------------------------------------------
Email: K.venner-at-ion.ucl.ac.uk Name: kerrie
Organization: ION, UCL UK
Title-Subject: [Filtered] sem prep of floating cell mats
Question: Never done this for sample prep, but it's a mind experiment: how about putting the cells and their media into a vessel like a bath with a plug at the bottom. Submerge the millipores and when they are sitting on the bottom, let the media out via the plug hole. I would say that the cell mats would settle gently with few creases. Sandwich lightly in a cut-down Swinnex holder, process and cpd intact. Just an idea from the old way I learned to coat grids with formvar.
Would not critical point drying them in one of our microporous specimen capsules be the best approach? See URL http://www.2spi.com/catalog/instruments/microporous.shtml
Chuck ----- Original Message ----- X-from: {peoshel-at-wisc.edu} To: {cgarber-at-2spi.com} Sent: Tuesday, July 19, 2005 10:13 AM
Chuck,
Well, he wants to try HMDS, and that works very well for bacteria. CPD is extra handling, and he's dealing with fragile biofilm mats, so that's another reason. It can work, but the less handling the better. I also don't use the porous capsules anymore. Too much trouble with staticy specimens after drying, and too many staticy little white bits of capsule coming off and getting on the specimens.
Phil
} Hello Phil, } } Would not critical point drying them in one of our microporous specimen } capsules be the best approach? See URL } http://www.2spi.com/catalog/instruments/microporous.shtml } } Chuck
} } Scott, } } } } Interesting problem. The "matts" you refer to are likely to fragment } } or worse if you use the usual filter-based preparation methods . } } First question: do you have access to cryoSEM? If yes, this would be } } the best way to do your samples. Freeze in a high-pressure freezer, } } if available, or by plunging into slush nitrogen, then do the cryo } } SEM. This is the method most likely to give you the unaltered } } structure of the matts and the critters therein. } } If you don't have access to cryoSEM, then the best way is to use } } minifuge tubes or the like. Let the samples sit in the tube in fix, } } then the EtOH dehydration steps, being very careful when you withdraw } } the fluid to change it. Add the fluid for the next step as you } } withdraw the old fluid to minimize disturbance of the matts, and } } maybe add the fluid for each succeeding step down the side of the } } tube. } } I suggest a 2:1 1:1 1:2 EtOH:HMDS series before the 3 changes in 100% } } HMDS. } } I've found it helpful to deposit the last HMDS + sample drops on a } } sputter-coated membrane filter* stuck to a SEM stub for drying. This } } further minimizes handling. } } * 0.22 micron "nucleopore" type -- the ones with the nice, round } } holes, not the torturous-path type of filter. Sputter coat both sides } } of the filter before sticking to the stub, best, or stick to the stub } } with conductive carbon tabs, then sputter coat. Then drop on the } } samples in HMDS. (And yes, sputter coat for viewing in the SEM.) } } } } Phil } } } } } Hi All, } } } } } } I need to prep some samples for SEM, but I'm not sure of the best way to } } } go about it. } } } } } } We have some bacteria that normally grow in a type of liquid nutrient } } } medium as free, discrete and seperate motile cells. However, we have } } } come across a strain that forms into 'mats' on the bottom of the culture } } } vessel. It is these that we would like to examine by SEM. } } } } } } The problem is that the 'mats' are not like a normal biofilm in that } } } they are not strongly adherant to a solid surface, and the mat is not } } } very robust. The mats will 'float' off the bottom of the culture vessel } } } if they are disturbed and they will fragment into small flakes if the } } } vessel is shaken or swirled. The fragments are usually a milimetre or so } } } square. } } } } } } Of course, the 'flakes' need to be flat in order to view them properly. } } } I thought I might be able to pipette them, in a drop of the media } } } they're already in, onto nucleopore membranes and allow them to settle, } } } then try to remove the rest of the media and hope that they stick } } } through the dehydration process... but i'm not sure they will. I'm } } } planning on using HMDS. } } } } } } I'm goig to try it just to see what happens... but has anyone prepared a } } } sample like this that could suggest something that is know to work well? } } } } } } Cheers, } } } } } } -- } } } Scott J. Coutts } } } --------------------------------------------------------------- } } } Bacterial Pathogenesis Research Group } } } Department of Microbiology, } } } Box 53, Monash University, 3800, Australia } } } Phone: 9905 4838 Fax: 9905 4811 } } } --------------------------------------------------------------- } } } } } } ==============================Original } } } Headers============================== } } } 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005 } } } 8, 17 -- Received: from omta04sl.mx.bigpond.com } } } (omta04sl.mx.bigpond.com [144.140.93.156]) } } } 8, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } } j6J6k1Km017389 } } } 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 } } } 01:46:02 -0500 } } } 8, 17 -- Received: from [60.224.29.18] by omta04sl.mx.bigpond.com with } } } ESMTP } } } 8, 17 -- id } } } {20050719064600.GCWB28893.omta04sl.mx.bigpond.com-at-[60.224.29.18]} } } } 8, 17 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2005 } } } 06:46:00 +0000 } } } 8, 17 -- Message-ID: {42DCA1AB.5070904-at-med.monash.edu.au} } } } 8, 17 -- Date: Tue, 19 Jul 2005 16:46:03 +1000 } } } 8, 17 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au} } } } 8, 17 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) } } } 8, 17 -- X-Accept-Language: en-us, en } } } 8, 17 -- MIME-Version: 1.0 } } } 8, 17 -- To: microscopy-at-microscopy.com } } } 8, 17 -- Subject: Another SEM sample prep question } } } 8, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } } 8, 17 -- Content-Transfer-Encoding: 7bit } } } ==============================End of - } } } Headers============================== } } } } -- } } Philip Oshel } } Supervisor, BBPIC microscopy facility } } Department of Animal Sciences } } University of Wisconsin } } 1675 Observatory Drive } } Madison, WI 53706 } } voice: (608) 263-4162 } } fax: (608) 262-5157 (dept. fax) } } http://www.ansci.wisc.edu/microscopy.htm } } } } ==============================Original } } Headers============================== } } 5, 27 -- From peoshel-at-wisc.edu Tue Jul 19 09:11:41 2005 } } 5, 27 -- Received: from smtp7.wiscmail.wisc.edu (hagen.doit.wisc.edu } } [144.92.197.163]) } } 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } j6JEBeEu028672 } } 5, 27 -- for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 6, 27 -- From peoshel-at-wisc.edu Wed Jul 20 08:20:17 2005 6, 27 -- Received: from smtp7.wiscmail.wisc.edu (hagen.doit.wisc.edu [144.92.197.163]) 6, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6KDKHLG023222 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2005 08:20:17 -0500 6, 27 -- Received: from avs-daemon.smtp7.wiscmail.wisc.edu by smtp7.wiscmail.wisc.edu 6, 27 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 6, 27 -- id {0IJX00J0TH1S6B-at-smtp7.wiscmail.wisc.edu} for microscopy-at-microscopy.com; 6, 27 -- Wed, 20 Jul 2005 08:20:16 -0500 (CDT) 6, 27 -- Received: from [10.25.102.29] (ansci.wisc.edu [144.92.132.175]) 6, 27 -- by smtp7.wiscmail.wisc.edu 6, 27 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 6, 27 -- with ESMTPSA id {0IJX0093MH1R0X-at-smtp7.wiscmail.wisc.edu} for 6, 27 -- microscopy-at-microscopy.com; Wed, 20 Jul 2005 08:20:15 -0500 (CDT) 6, 27 -- Date: Wed, 20 Jul 2005 08:20:15 -0500 6, 27 -- From: Philip Oshel {peoshel-at-wisc.edu} 6, 27 -- Subject: Re: [Microscopy] Another SEM sample prep question 6, 27 -- In-reply-to: {200507200424.j6K4OBPJ010895-at-ns.microscopy.com} 6, 27 -- X-Sender: peoshel-at-wiscmail.wisc.edu 6, 27 -- To: microscopy-at-microscopy.com 6, 27 -- Message-id: {p05210624bf03ff7b9db7-at-[10.25.102.29]} 6, 27 -- MIME-version: 1.0 6, 27 -- Content-type: text/plain; format=flowed; charset=us-ascii 6, 27 -- Content-transfer-encoding: 7BIT 6, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.132.175 6, 27 -- X-Spam-PmxInfo: Server=avs-4, Version=4.7.1.128075, Antispam-Engine: 2.0.3.1, 6, 27 -- Antispam-Data: 2005.7.20.10, SenderIP=144.92.132.175 6, 27 -- References: {200507200424.j6K4OBPJ010895-at-ns.microscopy.com} ==============================End of - Headers==============================
Do you want to use an imaging program or by x-ray microanalysis? If imaging, one shareware program you can use is Image J from NIH. Do a search for image j and it will take you to the download site. For simple measurements all you need to do is select the straight line tool, draw a line over the scale bar, and then set scale from a pull down menu. After that, with the same tool to draw a line over the distance you want to measure, keeping the mouse button down, and in the information field will be the line distance.
If by microanalysis, there is a program called GMR Film in the MSA library that you can download. It is based on K-ratio measurements so you need standards, and the film has to be smaller than the interaction volume. It is an old DOS program but works with XP, and I'm sure there are newer programs pout there too.
If you need any other help, feel free to contact me off-line, Lou Ross
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-- Senior Electron Microscope Specialist Electron Microscopy Core Facility W136 Veterinary Medicine University of Missouri Columbia, MO 65211-5120 (573) 882-4777, fax 884=2227 email: rosslm-at-missouri.edu http://www.emc.missouri.edu
==============================Original Headers============================== 8, 19 -- From RossLM-at-missouri.edu Wed Jul 20 08:35:35 2005 8, 19 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6KDZZ8R031181 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jul 2005 08:35:35 -0500 8, 19 -- Received: from um-exproto8.um.umsystem.edu ([207.160.151.148]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 8, 19 -- Wed, 20 Jul 2005 08:35:34 -0500 8, 19 -- Received: from [128.206.78.68] ([128.206.78.68]) by um-exproto8.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830); 8, 19 -- Wed, 20 Jul 2005 08:35:34 -0500 8, 19 -- Mime-Version: 1.0 8, 19 -- X-Sender: RossLM-at-pop.missouri.edu 8, 19 -- Message-Id: {p05200f5ebf040171a8a8-at-[128.206.78.68]} 8, 19 -- In-Reply-To: {1113382981.425ce0457fcde-at-webmail2.hut.fi} 8, 19 -- References: {1113382981.425ce0457fcde-at-webmail2.hut.fi} 8, 19 -- Date: Wed, 20 Jul 2005 08:35:33 -0500 8, 19 -- To: yezer-at-cc.hut.fi, Microscopy-at-microscopy.com 8, 19 -- From: Lou Ross {RossLM-at-missouri.edu} 8, 19 -- Subject: Re: [Microscopy] HR images with Hitachi S-4700 8, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 8, 19 -- X-OriginalArrivalTime: 20 Jul 2005 13:35:34.0422 (UTC) FILETIME=[E7F0BB60:01C58D2F] ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (westlab-at-linkline.com) from http://www.microscopy.com/MLFormMail.html on Wednesday, July 20, 2005 at 16:12:07 ---------------------------------------------------------------------------
Email: westlab-at-linkline.com Name: Mike Maladzhikyan
I am wondering if anyone has the Tegal Plasmod manual available? We acquired this etcher on the used market and it did not come with a manual. Please email me. I will be happy to pay you for your time and the copying costs. Thanks.
I've got one. It's only 8 pages long so it won't take long to scan it in. I'll send it shortly.
Diane Ciaburri
-----Original Message----- X-from: westlab-at-linkline.com [mailto:westlab-at-linkline.com] Sent: Wednesday, July 20, 2005 6:49 PM To: Ciaburri, Diane A
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (westlab-at-linkline.com) from http://www.microscopy.com/MLFormMail.html on Wednesday, July 20, 2005 at 16:12:07 ------------------------------------------------------------------------ ---
Email: westlab-at-linkline.com Name: Mike Maladzhikyan
I am wondering if anyone has the Tegal Plasmod manual available? We acquired this etcher on the used market and it did not come with a manual. Please email me. I will be happy to pay you for your time and the copying costs. Thanks.
Hi All, Here's the problem I hope someone out there can help me with.... I have a set of samples that I need to embed for TEM. I've tried previous sets, with poor results. The samples are cells plated on collagen gels or on fibronectin in those 35mm cover-slip bottomed dishes (available commercially). The cells have been pfa fixed, antibody labelled with peroidase-DAB as the chromogen, then fixed in 4% buffered glutaradehyde. The problem I've had is that in the end, the blocks have come out gummy rather than hard, so that they cannot be sectioned. I took some plain dishes and tested them out with both the Spurr's and LX-112. The blocks were fine, so the problem is not an interaction between the plastic of the dish and the resin (I embed by over-filling the BEEM capsule and inverting the sample over the meniscus of the resin). I have extended the dehydration steps to 2 changes of 15 minutes each at each of the ethanol concentrations, used a new bottle of 100% at the end, and left the samples to infiltrate with the resin before placing them in the oven. This week I tried 2 sample dishes. One seems like regions of the block face will cut well, but there may not be enough material to cut away a piece to re-embed so that I can cut both en face and cross sections. The second dish is gummy...the dish pulled off leaving a rough surface (vs snapping off and leaving a clean , smooth face). I can't use PO at the end of the dehydration because it will dissolve the dish, same for acetone as a dehydrant. Aside from moving to Phoenix (for both lower humidity and anonymity), I am at a loss. Any ideas? Thanks, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
==============================Original Headers============================== 1, 22 -- From lcgould-at-med.cornell.edu Thu Jul 21 13:46:10 2005 1, 22 -- Received: from smtp-in2.med.cornell.edu (smtp-in2.med.cornell.edu [140.251.0.25]) 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LIk9E6017497 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 13:46:10 -0500 1, 22 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 22 -- by smtp-in2.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6LIk2TB277600 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 14:46:06 -0400 1, 22 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu 1, 22 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 22 -- with ESMTP id {0IJZ00CPXQSP5640-at-mpx1.med.cornell.edu} for 1, 22 -- microscopy-at-microscopy.com; Thu, 21 Jul 2005 14:46:02 -0400 (EDT) 1, 22 -- Date: Thu, 21 Jul 2005 14:46:00 -0400 1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 22 -- Subject: Re: gummy blocks 1, 22 -- In-reply-to: {200507190325.j6J3PNU1025858-at-ns.microscopy.com} 1, 22 -- X-Sender: lcgould-at-pop.med.cornell.edu 1, 22 -- To: microscopy-at-microscopy.com 1, 22 -- Message-id: {p06020408bf058a73f04d-at-[140.251.145.131]} 1, 22 -- MIME-version: 1.0 1, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 22 -- References: {200507190325.j6J3PNU1025858-at-ns.microscopy.com} 1, 22 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.7.21.22 ==============================End of - Headers==============================
I have an old Simplex microscope (actually Leitz,) with one of the massive stands used for this type of measuring microscope. The micrometer height adjustment went out on me years ago and I've just been using the threaded height adjustor on the column to focus the scope. Now, I'm at a point where I really need the fine adjustment. Is there anyone out there who can disassemble the focusing rack that holds the scope and replace the small ball bearings so it will function again? If anyone can help, please let me know. Thanks.
Henry Barwood Associate Professor of Science, Earth Science Department of Math and Physics MSCX 312G Troy University Troy, Alabama 36082 hbarwood-at-troy.edu
==============================Original Headers============================== 3, 25 -- From hbarwood-at-troy.edu Thu Jul 21 14:02:29 2005 3, 25 -- Received: from webshield01.troy.edu (scan.troy.edu [198.179.130.124]) 3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6LJ2Ssa025405 3, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 21 Jul 2005 14:02:28 -0500 3, 25 -- Received: from (198.179.130.118) by webshield01.troy.edu via smtp 3, 25 -- id 377a_109524d2_fa1a_11d9_94ab_0002b3cdc1aa; 3, 25 -- Thu, 21 Jul 2005 19:03:03 +0000 (UTC) 3, 25 -- Received: from amy ([10.10.5.248]) 3, 25 -- by mail.troy.edu (MOS 3.5.6-GR) 3, 25 -- with ESMTP id BDK92258 (AUTH hbarwood); 3, 25 -- Thu, 21 Jul 2005 14:01:02 -0500 (CDT) 3, 25 -- From: "Henry Barwood" {hbarwood-at-troy.edu} 3, 25 -- To: {Microscopy-at-Microscopy.Com} 3, 25 -- Subject: Mechanical repair help? 3, 25 -- Date: Thu, 21 Jul 2005 14:07:21 -0500 3, 25 -- Message-ID: {NFBBLIEMIMFMNHKCOKOCEEHOFBAA.hbarwood-at-troy.edu} 3, 25 -- MIME-Version: 1.0 3, 25 -- Content-Type: text/plain; 3, 25 -- charset="iso-8859-1" 3, 25 -- Content-Transfer-Encoding: 7bit 3, 25 -- X-Priority: 3 (Normal) 3, 25 -- X-MSMail-Priority: Normal 3, 25 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 3, 25 -- Importance: Normal 3, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1409 ==============================End of - Headers==============================
Leona, What time and temperature did you use to cure the blocks? You can try using a lower temperature for a longer time to polymerize the blocks. That's worked for me when I've had gummy bears ~, ah, blocks, that is! Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W. Wiggins, E.M. Pathology Asst. CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron Microscopy, LLSPT, A823-A828 8700 Beverly Blvd. Los Angeles, CA 90048 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax) Winston.Wiggins-at-CSHS.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-----Original Message----- X-from: lcgould-at-med.cornell.edu [mailto:lcgould-at-med.cornell.edu] Sent: Thursday, July 21, 2005 11:51 AM To: Winston.Wiggins-at-CSHS.org
Hi All, Here's the problem I hope someone out there can help me with.... I have a set of samples that I need to embed for TEM. I've tried previous sets, with poor results. The samples are cells plated on collagen gels or on fibronectin in those 35mm cover-slip bottomed dishes (available commercially). The cells have been pfa fixed, antibody labelled with peroidase-DAB as the chromogen, then fixed in 4% buffered glutaradehyde. The problem I've had is that in the end, the blocks have come out gummy rather than hard, so that they cannot be sectioned. I took some plain dishes and tested them out with both the Spurr's and LX-112. The blocks were fine, so the problem is not an interaction between the plastic of the dish and the resin (I embed by over-filling the BEEM capsule and inverting the sample over the meniscus of the resin). I have extended the dehydration steps to 2 changes of 15 minutes each at each of the ethanol concentrations, used a new bottle of 100% at the end, and left the samples to infiltrate with the resin before placing them in the oven. This week I tried 2 sample dishes. One seems like regions of the block face will cut well, but there may not be enough material to cut away a piece to re-embed so that I can cut both en face and cross sections. The second dish is gummy...the dish pulled off leaving a rough surface (vs snapping off and leaving a clean , smooth face). I can't use PO at the end of the dehydration because it will dissolve the dish, same for acetone as a dehydrant. Aside from moving to Phoenix (for both lower humidity and anonymity), I am at a loss. Any ideas? Thanks, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
==============================Original Headers============================== 1, 22 -- From lcgould-at-med.cornell.edu Thu Jul 21 13:46:10 2005 1, 22 -- Received: from smtp-in2.med.cornell.edu (smtp-in2.med.cornell.edu [140.251.0.25]) 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LIk9E6017497 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 13:46:10 -0500 1, 22 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 22 -- by smtp-in2.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6LIk2TB277600 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 14:46:06 -0400 1, 22 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu 1, 22 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 22 -- with ESMTP id {0IJZ00CPXQSP5640-at-mpx1.med.cornell.edu} for 1, 22 -- microscopy-at-microscopy.com; Thu, 21 Jul 2005 14:46:02 -0400 (EDT) 1, 22 -- Date: Thu, 21 Jul 2005 14:46:00 -0400 1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 22 -- Subject: Re: gummy blocks 1, 22 -- In-reply-to: {200507190325.j6J3PNU1025858-at-ns.microscopy.com} 1, 22 -- X-Sender: lcgould-at-pop.med.cornell.edu 1, 22 -- To: microscopy-at-microscopy.com 1, 22 -- Message-id: {p06020408bf058a73f04d-at-[140.251.145.131]} 1, 22 -- MIME-version: 1.0 1, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 22 -- References: {200507190325.j6J3PNU1025858-at-ns.microscopy.com} 1, 22 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.7.21.22 ==============================End of - Headers==============================
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==============================Original Headers============================== 11, 24 -- From winston.wiggins-at-cshs.org Thu Jul 21 14:33:59 2005 11, 24 -- Received: from csip2.csmc.edu (CSIP2.csmc.edu [192.231.133.36]) 11, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LJXwAd001161 11, 24 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 14:33:59 -0500 11, 24 -- Received: from unknown (HELO zixvpm02.csmc.edu) (192.168.10.27) 11, 24 -- by csip2.csmc.edu with ESMTP; 21 Jul 2005 12:33:58 -0700 11, 24 -- X-BrightmailFiltered: true 11, 24 -- X-Brightmail-Tracker: AAAAAA== 11, 24 -- Received: from zixvpm02.csmc.edu (ZixVPM [127.0.0.1]) 11, 24 -- by Outbound.cshs.org (Proprietary) with ESMTP id B53644BE3B 11, 24 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 13:58:07 -0500 (CDT) 11, 24 -- Received: from exchange2.csmc.edu (SMTP.csmc.edu [166.124.43.200]) 11, 24 -- by zixvpm02.csmc.edu (Proprietary) with ESMTP id 84ECE4BE33 11, 24 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 13:58:07 -0500 (CDT) 11, 24 -- Received: by SMTP.csmc.edu with Internet Mail Service (5.5.2653.19) 11, 24 -- id {3SCR53PQ} ; Thu, 21 Jul 2005 12:33:57 -0700 11, 24 -- Message-ID: {B5AEAEDBB5D44C47BFE2A6D791329D7F2ADC75-at-EXCHANGE24.csmc.edu} 11, 24 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} 11, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 11, 24 -- Subject: RE: [Microscopy] Re: gummy blocks 11, 24 -- Date: Thu, 21 Jul 2005 12:33:53 -0700 11, 24 -- MIME-Version: 1.0 11, 24 -- X-Mailer: Internet Mail Service (5.5.2653.19) 11, 24 -- Content-Type: text/plain ==============================End of - Headers==============================
We have used Vashaw Scientific in Norcross, Ga. for service and repair on our Leica/Leitz microscopes. The service engineer's name is David Benjamin, phone number is 770-447-5632 and email address is "dbenjamin-at-vashaw.com"
I have no vested interest in Vashaw Scientific, but have enjoyed excellent sales support and service from them for over 20 years.
Good luck, Wade McFaddin Nextek Inc. Madison, Al.
-----Original Message----- X-from: hbarwood-at-troy.edu [mailto:hbarwood-at-troy.edu] Sent: Thursday, July 21, 2005 2:03 PM To: wade.mcfaddin-at-nextekinc.com
I have an old Simplex microscope (actually Leitz,) with one of the massive stands used for this type of measuring microscope. The micrometer height adjustment went out on me years ago and I've just been using the threaded height adjustor on the column to focus the scope. Now, I'm at a point where I really need the fine adjustment. Is there anyone out there who can disassemble the focusing rack that holds the scope and replace the small ball bearings so it will function again? If anyone can help, please let me know. Thanks.
Henry Barwood Associate Professor of Science, Earth Science Department of Math and Physics MSCX 312G Troy University Troy, Alabama 36082 hbarwood-at-troy.edu
==============================Original Headers============================== 3, 25 -- From hbarwood-at-troy.edu Thu Jul 21 14:02:29 2005 3, 25 -- Received: from webshield01.troy.edu (scan.troy.edu [198.179.130.124]) 3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6LJ2Ssa025405 3, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 21 Jul 2005 14:02:28 -0500 3, 25 -- Received: from (198.179.130.118) by webshield01.troy.edu via smtp 3, 25 -- id 377a_109524d2_fa1a_11d9_94ab_0002b3cdc1aa; 3, 25 -- Thu, 21 Jul 2005 19:03:03 +0000 (UTC) 3, 25 -- Received: from amy ([10.10.5.248]) 3, 25 -- by mail.troy.edu (MOS 3.5.6-GR) 3, 25 -- with ESMTP id BDK92258 (AUTH hbarwood); 3, 25 -- Thu, 21 Jul 2005 14:01:02 -0500 (CDT) 3, 25 -- From: "Henry Barwood" {hbarwood-at-troy.edu} 3, 25 -- To: {Microscopy-at-Microscopy.Com} 3, 25 -- Subject: Mechanical repair help? 3, 25 -- Date: Thu, 21 Jul 2005 14:07:21 -0500 3, 25 -- Message-ID: {NFBBLIEMIMFMNHKCOKOCEEHOFBAA.hbarwood-at-troy.edu} 3, 25 -- MIME-Version: 1.0 3, 25 -- Content-Type: text/plain; 3, 25 -- charset="iso-8859-1" 3, 25 -- Content-Transfer-Encoding: 7bit 3, 25 -- X-Priority: 3 (Normal) 3, 25 -- X-MSMail-Priority: Normal 3, 25 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 3, 25 -- Importance: Normal 3, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1409 ==============================End of - Headers==============================
I have found that at least one Spurr component (DER or ERL, maybe both) does react with plastic culture dishes over a few hours so that may be the cause. I tested the components individually, not the final mixture. I don't know about LX112. You can skip intermediate slovents like PO with Epon substitutes, Epon is miscible with ethanols even with some water remaining. When I did cultures I went from abs. EtOH to an EtOH:Epon mix (2:1 first, then 1:2 each for an hour) with agitation (slowly on a shaker table or just tilting the dish by hand every so often), then several changes of pure Epon several hours each, at least one under vacuum. You should also check your accelerator, replace if more than 6 months old. Also, I don't use DMP-30, I use BDMA instead. I don't see anything in your protocol that should cause problems. What happens if you allow the epoxy to polymerize by itself, no contact with culture dish?
Geoff
lcgould-at-med.cornell.edu wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 31 -- From mcauliff-at-umdnj.edu Thu Jul 21 14:44:06 2005 9, 31 -- Received: from mail01.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125]) 9, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LJi5VL016659 9, 31 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 14:44:06 -0500 9, 31 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1]) 9, 31 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 9B9E823007B 9, 31 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 15:44:05 -0400 (EDT) 9, 31 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 9, 31 -- by mail01.umdnj.edu (Proprietary) with ESMTP id A6CCF20C0B3 9, 31 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 15:44:02 -0400 (EDT) 9, 31 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 9, 31 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 9, 31 -- id {0IJZ00401SWPYU-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 9, 31 -- for microscopy-at-microscopy.com; Thu, 21 Jul 2005 15:44:02 -0400 (EDT) 9, 31 -- Received: from [127.0.0.1] ([10.138.2.240]) 9, 31 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 9, 31 -- 2004)) with ESMTP id {0IJZ002ZZSODR7-at-Polaris.umdnj.edu} for 9, 31 -- microscopy-at-microscopy.com; Thu, 21 Jul 2005 15:26:37 -0400 (EDT) 9, 31 -- Date: Thu, 21 Jul 2005 15:26:39 -0400 9, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 31 -- Subject: Re: [Microscopy] Re: gummy blocks 9, 31 -- In-reply-to: {200507211848.j6LIm6tH019703-at-ns.microscopy.com} 9, 31 -- To: microscopy-at-microscopy.com 9, 31 -- Message-id: {42DFF6EF.1000502-at-umdnj.edu} 9, 31 -- MIME-version: 1.0 9, 31 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 9, 31 -- Content-transfer-encoding: 7BIT 9, 31 -- X-Accept-Language: en-us, en 9, 31 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 9, 31 -- Gecko/20040804 Netscape/7.2 (ax) 9, 31 -- References: {200507211848.j6LIm6tH019703-at-ns.microscopy.com} ==============================End of - Headers==============================
I've had this problem with cultures grown on Matrigel- as well as plain old collagen-coated plates. These substrates are very hydrated, so extending your dehydration times and doing a few extra changes of absolute, dry ethanol (or acetone) at the end should take care of the gummy-block syndrome.
Good luck!
Tamara
On Thu, 21 Jul 2005 lcgould-at-med.cornell.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } Here's the problem I hope someone out there can help me with.... } I have a set of samples that I need to embed for TEM. I've tried } previous sets, with poor results. The samples are cells plated on } collagen gels or on fibronectin in those 35mm cover-slip bottomed } dishes (available commercially). The cells have been pfa fixed, } antibody labelled with peroidase-DAB as the chromogen, then fixed in } 4% buffered glutaradehyde. The problem I've had is that in the end, } the blocks have come out gummy rather than hard, so that they cannot } be sectioned. } I took some plain dishes and tested them out with both the Spurr's } and LX-112. The blocks were fine, so the problem is not an } interaction between the plastic of the dish and the resin (I embed by } over-filling the BEEM capsule and inverting the sample over the } meniscus of the resin). } I have extended the dehydration steps to 2 changes of 15 minutes each } at each of the ethanol concentrations, used a new bottle of 100% at } the end, and left the samples to infiltrate with the resin before } placing them in the oven. } This week I tried 2 sample dishes. One seems like regions of the } block face will cut well, but there may not be enough material to cut } away a piece to re-embed so that I can cut both en face and cross } sections. The second dish is gummy...the dish pulled off leaving a } rough surface (vs snapping off and leaving a clean , smooth face). } I can't use PO at the end of the dehydration because it will dissolve } the dish, same for acetone as a dehydrant. Aside from moving to } Phoenix (for both lower humidity and anonymity), I am at a loss. } Any ideas? } Thanks, } Lee } -- } Leona Cohen-Gould, M.S. } Sr. Staff Associate } Director, Electron Microscopy & Histology Core Facility } Manager, Optical Microscopy Core Facility } Joan & Sanford I. Weill Medical College } of Cornell University } voice (212)746-6146 } fax (212)746-8175 } } ==============================Original Headers============================== } 1, 22 -- From lcgould-at-med.cornell.edu Thu Jul 21 13:46:10 2005 } 1, 22 -- Received: from smtp-in2.med.cornell.edu (smtp-in2.med.cornell.edu [140.251.0.25]) } 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LIk9E6017497 } 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 13:46:10 -0500 } 1, 22 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) } 1, 22 -- by smtp-in2.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6LIk2TB277600 } 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 14:46:06 -0400 } 1, 22 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu } 1, 22 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) } 1, 22 -- with ESMTP id {0IJZ00CPXQSP5640-at-mpx1.med.cornell.edu} for } 1, 22 -- microscopy-at-microscopy.com; Thu, 21 Jul 2005 14:46:02 -0400 (EDT) } 1, 22 -- Date: Thu, 21 Jul 2005 14:46:00 -0400 } 1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} } 1, 22 -- Subject: Re: gummy blocks } 1, 22 -- In-reply-to: {200507190325.j6J3PNU1025858-at-ns.microscopy.com} } 1, 22 -- X-Sender: lcgould-at-pop.med.cornell.edu } 1, 22 -- To: microscopy-at-microscopy.com } 1, 22 -- Message-id: {p06020408bf058a73f04d-at-[140.251.145.131]} } 1, 22 -- MIME-version: 1.0 } 1, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed } 1, 22 -- References: {200507190325.j6J3PNU1025858-at-ns.microscopy.com} } 1, 22 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.7.21.22 } ==============================End of - Headers============================== }
|--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward-at-unm.edu |--------------------------------------------------|
==============================Original Headers============================== 6, 16 -- From thoward-at-unm.edu Thu Jul 21 14:50:25 2005 6, 16 -- Received: from phact.unm.edu (f5vs2.unm.edu [64.106.76.41]) 6, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LJoPpt024390 6, 16 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 14:50:25 -0500 6, 16 -- Received: from [129.24.9.44] (HELO egor.unm.edu) 6, 16 -- by phact.unm.edu (CommuniGate Pro SMTP 4.2.7) 6, 16 -- with ESMTP-TLS id 23682686 for microscopy-at-microscopy.com; Thu, 21 Jul 2005 13:50:24 -0600 6, 16 -- Date: Thu, 21 Jul 2005 13:50:24 -0600 (MDT) 6, 16 -- From: Tamara Howard {thoward-at-unm.edu} 6, 16 -- To: microscopy-at-microscopy.com 6, 16 -- Subject: Re: [Microscopy] Re: gummy blocks 6, 16 -- In-Reply-To: {200507211848.j6LImQOc020124-at-ns.microscopy.com} 6, 16 -- Message-ID: {Pine.LNX.4.62.0507211346180.18821-at-egor.unm.edu} 6, 16 -- References: {200507211848.j6LImQOc020124-at-ns.microscopy.com} 6, 16 -- MIME-Version: 1.0 6, 16 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Hi Lee .Can you remove the coverslip from the dish after fixation and process it alone? That way you could embed the whole coverslip in a Chang mold, remove the glass using HF and punch out cells from the wafer. You could also re-embed chips to cross-section. You would avoid the dish problem. I have a feeling that your problems could be due to solutions getting trapped under the coverslip (I am not familiar with this type of dish) or sometimes the coating on the coverslip causes problems with removing the inverted Beem capsules. The whole coating comes off leaving the rough surface you mention. You might also want to check your resin and make sure it is freshly made. Good luck, JoAnn 01:49 PM 7/21/2005 -0500, you wrote:
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Department of Molecular and Cellular Physiology Stanford University School of Medicine Stanford, CA 94305 650-723-5856
==============================Original Headers============================== 6, 17 -- From redhair-at-stanford.edu Thu Jul 21 16:03:40 2005 6, 17 -- Received: from smtp2.Stanford.EDU (smtp2.Stanford.EDU [171.67.16.125]) 6, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LL3ejW000829 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 16:03:40 -0500 6, 17 -- Received: from Bucky.stanford.edu (B135-WinXP.Stanford.EDU [171.65.21.62]) 6, 17 -- by smtp2.Stanford.EDU (8.12.11/8.12.11) with ESMTP id j6LL3bYd007200 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 14:03:38 -0700 6, 17 -- Message-Id: {5.1.0.14.2.20050721124755.05ee2ca0-at-redhair.pobox.stanford.edu} 6, 17 -- X-Sender: redhair-at-redhair.pobox.stanford.edu (Unverified) 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 5.1 6, 17 -- Date: Thu, 21 Jul 2005 14:03:35 -0700 6, 17 -- To: microscopy-at-microscopy.com 6, 17 -- From: JoAnn Buchanan {redhair-at-stanford.edu} 6, 17 -- Subject: Re: [Microscopy] Re: gummy blocks 6, 17 -- In-Reply-To: {200507211849.j6LIngjK022126-at-ns.microscopy.com} 6, 17 -- Mime-Version: 1.0 6, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I will be out of the lab from Friday, July 22 through Friday, August 5, 2005.
If you need to speak with someone regarding the Microscopy and Imaging Core Facility, please contact our microscopy specialist, Rhonda Grebe, at 5-2597 for assistance; otherwise I will get back to you on Monday, August 8.
Mary Ellen
==============================Original Headers============================== 4, 26 -- From Mailer-Daemon-at-cis27.hosts.jhmi.edu Thu Jul 21 16:08:21 2005 4, 26 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141]) 4, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LL8LUj008588 4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 16:08:21 -0500 4, 26 -- Received: from cis27.hosts.jhmi.edu (162.129.9.14) 4, 26 -- by ipex1.johnshopkins.edu with ESMTP; 21 Jul 2005 17:08:20 -0400 4, 26 -- X-BrightmailFiltered: true 4, 26 -- X-Brightmail-Tracker: AAAAAA== 4, 26 -- X-IronPort-AV: i="3.95,134,1120449600"; 4, 26 -- d="scan'208"; a="78363069:sNHT21560736" 4, 26 -- Received: from Jhmipri-MTA by cis27.hosts.jhmi.edu 4, 26 -- with Novell_GroupWise; Thu, 21 Jul 2005 17:08:20 -0400 4, 26 -- Message-Id: {s2dfd684.077-at-cis27.hosts.jhmi.edu} 4, 26 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 4, 26 -- Date: Thu, 21 Jul 2005 17:08:06 -0400 4, 26 -- From: "Mary Ellen Pease" {mpease-at-jhmi.edu} 4, 26 -- Sender: Postmaster-at-jhmi.edu 4, 26 -- Reply-To: mpease-at-jhmi.edu 4, 26 -- Errors-To: Postmaster-at-jhmi.edu 4, 26 -- To: {microscopy-at-microscopy.com} 4, 26 -- Subject: Re: [Microscopy] gummy blocks (Out of the office/lab) 4, 26 -- Mime-Version: 1.0 4, 26 -- Content-Type: text/plain; charset=US-ASCII 4, 26 -- Content-Disposition: inline 4, 26 -- Content-Transfer-Encoding: 8bit 4, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6LL8LUj008588 ==============================End of - Headers==============================
Lee, Those small well dishes do not do so well when trying to exchange fluids. I have a few suggestions and you may wish to try one or all.
1. I use a rotating table so that the fluids are swirling during the time that they are sitting. 2. The dehydration times for single cells seems fine but adding the gel with cells on top is equivalent to thin blocks so that I would suggest doubling the time in 100% EtOH. 3. For many years I have been using HPMA (Hydroxypropyl Methacrylate)in place of Propylene Oxide both after the 100% EtOH and in a 1:1 and a 2:1::Epon:HPMA mixture before going into 100% Epon at least 3 times before embedding.
I do not know the reference for using HPMA. I was instructed to use it in plastic Tissue Culture dishes when I first learned embedding back in 1971. (Thanks to whomever the first one was who tried it!)
I agree that the LX-112 works great as the substitute for the original epon which does not melt the plastics that the dishes are made from.
Pat Connelly Univ. of Pennsylvania psconnel-at-sas.upenn.edu
} Here's the problem I hope someone out there can help me with.... } I have a set of samples that I need to embed for TEM. I've tried } previous sets, with poor results. The samples are cells plated on } collagen gels or on fibronectin in those 35mm cover-slip bottomed } dishes (available commercially). The cells have been pfa fixed, } antibody labelled with peroidase-DAB as the chromogen, then fixed in } 4% buffered glutaradehyde. The problem I've had is that in the end, } the blocks have come out gummy rather than hard, so that they cannot } be sectioned. } I took some plain dishes and tested them out with both the Spurr's } and LX-112. The blocks were fine, so the problem is not an } interaction between the plastic of the dish and the resin (I embed by } over-filling the BEEM capsule and inverting the sample over the } meniscus of the resin). } I have extended the dehydration steps to 2 changes of 15 minutes each } at each of the ethanol concentrations, used a new bottle of 100% at } the end, and left the samples to infiltrate with the resin before } placing them in the oven. } This week I tried 2 sample dishes. One seems like regions of the } block face will cut well, but there may not be enough material to cut } away a piece to re-embed so that I can cut both en face and cross } sections. The second dish is gummy...the dish pulled off leaving a } rough surface (vs snapping off and leaving a clean , smooth face). } I can't use PO at the end of the dehydration because it will dissolve } the dish, same for acetone as a dehydrant. Aside from moving to } Phoenix (for both lower humidity and anonymity), I am at a loss. } Thanks, } Lee } -- } Leona Cohen-Gould, M.S. } Sr. Staff Associate } Director, Electron Microscopy & Histology Core Facility } Manager, Optical Microscopy Core Facility } Joan & Sanford I. Weill Medical College of Cornell University
==============================Original Headers============================== 7, 25 -- From psconnel-at-sas.upenn.edu Thu Jul 21 16:46:31 2005 7, 25 -- Received: from webmail2.sas.upenn.edu (webmail.SAS.UPENN.EDU [128.91.55.36]) 7, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LLkVQS016811 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 16:46:31 -0500 7, 25 -- Received: from webmail2.sas.upenn.edu (localhost.localdomain [127.0.0.1]) 7, 25 -- by webmail2.sas.upenn.edu (8.12.8/8.12.8) with ESMTP id j6LLkUcr026695 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 17:46:30 -0400 7, 25 -- Received: (from nobody-at-localhost) 7, 25 -- by webmail2.sas.upenn.edu (8.12.8/8.12.8/Submit) id j6LLkU3p026692 7, 25 -- for microscopy-at-microscopy.com; Thu, 21 Jul 2005 17:46:30 -0400 7, 25 -- X-Authentication-Warning: webmail2.sas.upenn.edu: nobody set sender to psconnel-at-sas.upenn.edu using -f 7, 25 -- Received: from DEPT-DYNAMIC-HOST027.BIO.UPENN.EDU (DEPT-DYNAMIC-HOST027.BIO.UPENN.EDU [130.91.131.27]) 7, 25 -- by webmail.sas.upenn.edu (IMP) with HTTP 7, 25 -- for {psconnel-at-127.0.0.1} ; Thu, 21 Jul 2005 17:46:30 -0400 7, 25 -- Message-ID: {1121982390.42e017b672b1f-at-webmail.sas.upenn.edu} 7, 25 -- Date: Thu, 21 Jul 2005 17:46:30 -0400 7, 25 -- From: psconnel-at-sas.upenn.edu 7, 25 -- To: microscopy-at-microscopy.com 7, 25 -- Subject: Re: [Microscopy] Re: gummy blocks 7, 25 -- References: {200507211848.j6LIm7cW019724-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {200507211848.j6LIm7cW019724-at-ns.microscopy.com} 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- User-Agent: Internet Messaging Program (IMP) 3.2.7 ==============================End of - Headers==============================
The stage on my JSM 840A is pushed by linkage from the micrometer knob in one X direction, and pulled back by a spring in the other X direction.
It has become a bit sticky, so that the spring pulls it back only slowly. Some users find this disconcerting.
Should I clean it or lubricate it?
If the latter, with what?
Diff pump oil?
cheers
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 11, 28 -- From r.sims-at-auckland.ac.nz Thu Jul 21 17:10:41 2005 11, 28 -- Received: from smtpa.itss.auckland.ac.nz (mailhost.auckland.ac.nz [130.216.190.11]) 11, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6LMAfmH024666 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2005 17:10:41 -0500 11, 28 -- Received: from localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) 11, 28 -- by smtpa.itss.auckland.ac.nz (Postfix) with ESMTP id 4E0CD34DF7 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 10:10:40 +1200 (NZST) 11, 28 -- Received: from smtpa.itss.auckland.ac.nz ([127.0.0.1]) 11, 28 -- by localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id 21171-26 for {microscopy-at-microscopy.com} ; 11, 28 -- Fri, 22 Jul 2005 10:10:40 +1200 (NZST) 11, 28 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 11, 28 -- by smtpa.itss.auckland.ac.nz (Postfix) with ESMTP id 30CE534B1C 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 10:10:40 +1200 (NZST) 11, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 11, 28 -- Organization: Dept of Geology, Univ of Auckland 11, 28 -- To: microscopy-at-microscopy.com 11, 28 -- Date: Fri, 22 Jul 2005 10:11:54 +1200 11, 28 -- MIME-Version: 1.0 11, 28 -- Subject: Sticky Stage on 840A 11, 28 -- Message-ID: {42E0C620.7833.5A7682-at-localhost} 11, 28 -- Priority: normal 11, 28 -- In-reply-to: {200507122213.j6CMDjxZ016323-at-ns.microscopy.com} 11, 28 -- X-mailer: Pegasus Mail for Windows (4.21c) 11, 28 -- Content-type: text/plain; charset=US-ASCII 11, 28 -- Content-transfer-encoding: 7BIT 11, 28 -- Content-description: Mail message body 11, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
Dear Ritchie, I find that if you take off the micrometer or undo the gears inside and take the shaft right out of the feedthrough, you can clean the stiff black stuff off the shaft with lab alcohol. Take out the o-ring and clean it and its groove and then lubricate the o-ring lightly with Apiazon L high vacuum grease. Check that the spring isn't gummed up, as well. If you cannot get the shaft out, run it to one end and clean all that is exposed and then run it to the other end and clean the rest. If you cannot get out the o-ring, you can put a bit of high vacuum grease on the clean shaft and then run it into the o-ring hole. ----- Original Message ----- X-from: {r.sims-at-auckland.ac.nz} To: {mager-at-interchange.ubc.ca} Sent: Thursday, July 21, 2005 3:14 PM
Hi Ritchie,
Had the same problem a while back on our 5600. Our serviceman told me to use a bit of RP oil. I took the micrometer completely off and ran it through it's entire travel, adding a small amount (just a few drops total) as I went. It didn't really help at first, but then it must have coated the sticky part, because it became silky smooth again. It appears, though, that once they start doing this, they need to have this done every year or so. Mine is starting to get a little stiff again.
Hope this helps,
Jim
r.sims-at-auckland.ac.nz wrote:
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James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
Ritchie, Your problem is more than likely caused by a sticky universal "slip joint" that connects the external X micrometer with the X stage slide, not a sticky X bearing slide. You can check this by moving the universal itself in a back & forth motion. I would guess that the "Y" universal moves freely and the X universal is sticky. Or, I have seen those anti-backlash springs get weak after 20 years or so. And , lubricating your stage with anything, even dry graphite is not a good thing to do with regards to your vacuum system (cleanliness, ultimate vacuum, sample contamination, etc).
Gary M. Easton Scanners Corporation 3rd Party SEM Service
r.sims-at-auckland.ac.nz wrote:
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (qxing-at-uno.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 21, 2005 at 12:25:38 ---------------------------------------------------------------------------
Email: qxing-at-uno.edu Name: Qingfeng Xing
Organization: University of New Orleans
Education: Graduate College
Location: New Orleans LA 70148
Question: How to index high order Kikuchi lines of silicon?
Clear high order Kikuchi lines can be obtained by CBED at an orientation far from low-index zones. I found that it's difficult to index them even knowing the position of the direct beam on the Kickuchi map, as there are so many lines. Is there any procedure to do that?
We plan to make a modification for our optical motorized microscope and consider an option to go with Nikon D-70. If any one could share experience to adopt such camera for science, would you please share with me? There is any software, which could control the camera? Any preferences? Any other sources of information? There is another cameras/software suitable for such upgrade?
Thank you very much, Vlad
==============================Original Headers============================== 5, 20 -- From uti-at-direcpc.com Fri Jul 22 06:21:10 2005 5, 20 -- Received: from a34-mta02.direcway.com (a34-mta02.direcpc.com [66.82.4.91]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MBLAap007989 5, 20 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 06:21:10 -0500 5, 20 -- Received: from ringo.direcpc.com (dpclt036170.direcpc.com [64.157.36.170]) 5, 20 -- by a34-mta02.direcway.com 5, 20 -- (iPlanet Messaging Server 5.2 HotFix 1.25 (built Mar 3 2004)) 5, 20 -- with ESMTP id {0IK1004IP0UWRF-at-a34-mta02.direcway.com} for 5, 20 -- microscopy-at-microscopy.com; Fri, 22 Jul 2005 07:21:06 -0400 (EDT) 5, 20 -- Date: Fri, 22 Jul 2005 07:20:33 -0400 5, 20 -- From: UTI {uti-at-direcpc.com} 5, 20 -- Subject: Nikon D-70 for optical microscopy 5, 20 -- In-reply-to: {200507220307.j6M371Y0002010-at-ns.microscopy.com} 5, 20 -- X-Sender: uti-at-pop3.direcpc.com 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Message-id: {5.1.0.14.2.20050722070947.01e5ba10-at-pop3.direcpc.com} 5, 20 -- MIME-version: 1.0 5, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.1 5, 20 -- Content-type: text/plain; charset=us-ascii; format=flowed 5, 20 -- Content-transfer-encoding: 7BIT ==============================End of - Headers==============================
To add oil to your stage is nothing more than accelerated back-streaming. If the stage is sticking or "jumpy" when translating the sample, the best approach is to minimally disassemble, thoroughly clean, re-surface ball bearing races where necessary, inspect for worn parts, and reassemble as it was.
Bob Roberts EM Lab Services, Inc. 449 NW 62nd St. Topeka, Kansas 66617-1780 785.246.1232 voice 785.246.0168 fax www.emlabservices.com
==============================Original Headers============================== 5, 23 -- From emlabservices-at-cox.net Fri Jul 22 06:29:20 2005 5, 23 -- Received: from centrmmtai03.cox.net ([68.1.16.131]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MBTJ4r015977 5, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 06:29:20 -0500 5, 23 -- Received: from EMLabServices ([24.255.213.54]) by lakermmtao06.cox.net 5, 23 -- (InterMail vM.6.01.04.00 201-2131-118-20041027) with SMTP 5, 23 -- id {20050722112844.UWJ26250.lakermmtao06.cox.net-at-EMLabServices} 5, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 07:28:44 -0400 5, 23 -- Message-ID: {002e01c58eb0$8bee3680$6400a8c0-at-EMLabServices} 5, 23 -- From: "EM Lab Services" {emlabservices-at-cox.net} 5, 23 -- To: {Microscopy-at-microscopy.com} 5, 23 -- Subject: SEM: 840A Sticky Stage 5, 23 -- Date: Fri, 22 Jul 2005 04:28:55 -0700 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- format=flowed; 5, 23 -- charset="iso-8859-1"; 5, 23 -- reply-type=original 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Priority: 3 5, 23 -- X-MSMail-Priority: Normal 5, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 5, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
You can get various programs to simulate the HOLZ lines. You can also use the on-line resources developed by Stadelmann at Lausanne. The webaddress is:
-----Original Message----- X-from: qxing-at-uno.edu [mailto:qxing-at-uno.edu] Sent: 22. juli 2005 05:04 To: j.bilde-at-risoe.dk
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (qxing-at-uno.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 21, 2005 at 12:25:38 ---------------------------------------------------------------------------
Email: qxing-at-uno.edu Name: Qingfeng Xing
Organization: University of New Orleans
Education: Graduate College
Location: New Orleans LA 70148
Question: How to index high order Kikuchi lines of silicon?
Clear high order Kikuchi lines can be obtained by CBED at an orientation far from low-index zones. I found that it's difficult to index them even knowing the position of the direct beam on the Kickuchi map, as there are so many lines. Is there any procedure to do that?
Ask Prof. Zhou Weilie at UNO. He knows the answer.
---- Original message ---- } Date: Thu, 21 Jul 2005 22:04:03 -0500 } From: qxing-at-uno.edu } Subject: [Microscopy] AskAMicroscopist: How to index high order Kikuchi lines of } To: clei-at-uiuc.edu } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 4, 26 -- From clei-at-uiuc.edu Fri Jul 22 08:41:49 2005 4, 26 -- Received: from expredir3.cites.uiuc.edu (expredir3.cites.uiuc.edu [128.174.5.186]) 4, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MDfnGG000844 4, 26 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 08:41:49 -0500 4, 26 -- Received: from expms6.cites.uiuc.edu (expms6.cites.uiuc.edu [128.174.5.43]) 4, 26 -- by expredir3.cites.uiuc.edu (8.12.11/8.12.11) with ESMTP id j6MDfnjs010195 4, 26 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 08:41:49 -0500 (CDT) 4, 26 -- Received: from expms6.cites.uiuc.edu (localhost.cites.uiuc.edu [127.0.0.1]) 4, 26 -- by expms6.cites.uiuc.edu (MOS 3.4.8-GR) 4, 26 -- with ESMTP id AVA28490; 4, 26 -- Fri, 22 Jul 2005 08:41:48 -0500 (CDT) 4, 26 -- Received: from 128.174.5.212 4, 26 -- by expms6.cites.uiuc.edu (MOS 3.4.8-GR) 4, 26 -- with HTTP/1.1; 4, 26 -- Fri, 22 Jul 2005 07:41:48 -0600 4, 26 -- Date: Fri, 22 Jul 2005 07:41:48 -0600 4, 26 -- From: Changhui LEI {clei-at-uiuc.edu} 4, 26 -- Subject: Re: [Microscopy] AskAMicroscopist: How to 4, 26 -- index high order Kikuchi lines of 4, 26 -- To: microscopy-at-microscopy.com 4, 26 -- Reply-To: clei-at-uiuc.edu 4, 26 -- X-Mailer: Webmail Mirapoint Direct 3.4.8-GR 4, 26 -- MIME-Version: 1.0 4, 26 -- Message-Id: {347707fd.1fe09339.81bc500-at-expms6.cites.uiuc.edu} 4, 26 -- Content-Type: text/plain; charset=us-ascii 4, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
i haven't had the time to follow the thread so if someone has suggested this then just ignore. you can buy polyproplene dishes that work just as well so you can use PO, there are also polypropylene cover slips as well as slide, i thing the use of PO will solve your gummy blocks john
--- lcgould-at-med.cornell.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } Here's the problem I hope someone out there can } help me with.... } I have a set of samples that I need to embed for } TEM. I've tried } previous sets, with poor results. The samples are } cells plated on } collagen gels or on fibronectin in those 35mm } cover-slip bottomed } dishes (available commercially). The cells have been } pfa fixed, } antibody labelled with peroidase-DAB as the } chromogen, then fixed in } 4% buffered glutaradehyde. The problem I've had is } that in the end, } the blocks have come out gummy rather than hard, so } that they cannot } be sectioned. } I took some plain dishes and tested them out with } both the Spurr's } and LX-112. The blocks were fine, so the problem is } not an } interaction between the plastic of the dish and the } resin (I embed by } over-filling the BEEM capsule and inverting the } sample over the } meniscus of the resin). } I have extended the dehydration steps to 2 changes } of 15 minutes each } at each of the ethanol concentrations, used a new } bottle of 100% at } the end, and left the samples to infiltrate with the } resin before } placing them in the oven. } This week I tried 2 sample dishes. One seems like } regions of the } block face will cut well, but there may not be } enough material to cut } away a piece to re-embed so that I can cut both en } face and cross } sections. The second dish is gummy...the dish pulled } off leaving a } rough surface (vs snapping off and leaving a clean , } smooth face). } I can't use PO at the end of the dehydration because } it will dissolve } the dish, same for acetone as a dehydrant. Aside } from moving to } Phoenix (for both lower humidity and anonymity), I } am at a loss. } Any ideas? } Thanks, } Lee } -- } Leona Cohen-Gould, M.S. } Sr. Staff Associate } Director, Electron Microscopy & Histology Core } Facility } Manager, Optical Microscopy Core Facility } Joan & Sanford I. Weill Medical College } of Cornell University } voice (212)746-6146 } fax (212)746-8175 } } ==============================Original } Headers============================== } 1, 22 -- From lcgould-at-med.cornell.edu Thu Jul 21 } 13:46:10 2005 } 1, 22 -- Received: from smtp-in2.med.cornell.edu } (smtp-in2.med.cornell.edu [140.251.0.25]) } 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j6LIk9E6017497 } 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 } Jul 2005 13:46:10 -0500 } 1, 22 -- Received: from mpx1.med.cornell.edu } (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) } 1, 22 -- by smtp-in2.med.cornell.edu } (Switch-3.1.6/Switch-3.1.6) with ESMTP id } j6LIk2TB277600 } 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 } Jul 2005 14:46:06 -0400 } 1, 22 -- Received: from [140.251.145.131] by } mpx1.med.cornell.edu } 1, 22 -- (Sun Java System Messaging Server 6.1 } HotFix 0.11 (built Jan 28 2005)) } 1, 22 -- with ESMTP id } {0IJZ00CPXQSP5640-at-mpx1.med.cornell.edu} for } 1, 22 -- microscopy-at-microscopy.com; Thu, 21 Jul } 2005 14:46:02 -0400 (EDT) } 1, 22 -- Date: Thu, 21 Jul 2005 14:46:00 -0400 } 1, 22 -- From: Leona Cohen-Gould } {lcgould-at-med.cornell.edu} } 1, 22 -- Subject: Re: gummy blocks } 1, 22 -- In-reply-to: } {200507190325.j6J3PNU1025858-at-ns.microscopy.com} } 1, 22 -- X-Sender: lcgould-at-pop.med.cornell.edu } 1, 22 -- To: microscopy-at-microscopy.com } 1, 22 -- Message-id: } {p06020408bf058a73f04d-at-[140.251.145.131]} } 1, 22 -- MIME-version: 1.0 } 1, 22 -- Content-type: text/plain; charset=us-ascii; } format=flowed } 1, 22 -- References: } {200507190325.j6J3PNU1025858-at-ns.microscopy.com} } 1, 22 -- X-PMX-Version: 4.7.1.128075, } Antispam-Engine: 2.0.3.2, Antispam-Data: } 2005.7.21.22 } ==============================End of - } Headers============================== }
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I only saw one response to your question, so I thought that I would jump in and try to help. As was pointed out in the posting from Jorgen Bilde, you can get a program to help you, but you can also do it manually with some effort.
If you know the orientation of the beam, then you can figure out the range of indices that match or closely matches the g "dot" =1 or 2 condition (where B is the beam direction). You have to know the angular half angle to calculate the range of indices to use. Once you find the indices, you sort them to find the order from the exact Bragg condition (center of disk).
In Mike Kersker's (JEOL, USA) Ph.D. dissertation from , there is a nice demonstration on how to do this with an ordered alloy phase.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: qxing-at-uno.edu [mailto:qxing-at-uno.edu] Sent: Thursday, July 21, 2005 8:07 PM To: Walck-at-SouthBayTech.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (qxing-at-uno.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 21, 2005 at 12:25:38 ------------------------------------------------------------------------ ---
Email: qxing-at-uno.edu Name: Qingfeng Xing
Organization: University of New Orleans
Education: Graduate College
Location: New Orleans LA 70148
Question: How to index high order Kikuchi lines of silicon?
Clear high order Kikuchi lines can be obtained by CBED at an orientation far from low-index zones. I found that it's difficult to index them even knowing the position of the direct beam on the Kickuchi map, as there are so many lines. Is there any procedure to do that?
See if these guys can help you out: Molecular and Cellular Imaging Facility K1 Building University of Texas Southwestern Medical Center at Dallas 5323 Harry Hines Blvd Dallas, TX 5390-9039
Office for Christopher Gilpin Ph.D. 214.648.2827
Lab for George Lawton or Tom Januszewski (214) 648-7291
rbeavers-at-mail.smu.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Group, } } Trying to locate an environmental SEM (meaning I want to look at materials that have liquid solutions as a component) in the North Texas area. } } Thanks } } Roy Beavers } } Southern Methodist University } Department of Geological Sciences } P.O. Box 750395 } Dallas, TX 75275 } Voice: 214-768-2756 } Fax: 214-768-2701 } Email: rbeavers-at-smu.edu } } } } ==============================Original Headers============================== } 7, 20 -- From rbeavers-at-mail.smu.edu Fri Jul 22 12:24:38 2005 } 7, 20 -- Received: from s31xe5.systems.smu.edu (s31xe5.systems.smu.edu [129.119.70.74]) } 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MHOcDu027467 } 7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 12:24:38 -0500 } 7, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 7, 20 -- Content-class: urn:content-classes:message } 7, 20 -- MIME-Version: 1.0 } 7, 20 -- Content-Type: text/plain; } 7, 20 -- charset="iso-8859-1" } 7, 20 -- Subject: Environmental SEM in North Texas } 7, 20 -- Date: Fri, 22 Jul 2005 12:24:37 -0500 } 7, 20 -- Message-ID: {3A9F6E299461A44483961A57175598A71AD105-at-s31xe5.systems.smu.edu} } 7, 20 -- X-MS-Has-Attach: } 7, 20 -- X-MS-TNEF-Correlator: } 7, 20 -- Thread-Topic: Environmental SEM in North Texas } 7, 20 -- Thread-Index: AcWO4jyJWcNbkeqYTzS+WV+10DjcJA== } 7, 20 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} } 7, 20 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} } 7, 20 -- Content-Transfer-Encoding: 8bit } 7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6MHOcDu027467 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 27 -- From jpflugheber-at-stlawu.edu Fri Jul 22 13:08:22 2005 8, 27 -- Received: from mx2.stlawu.edu (mx2.stlawu.edu [199.0.76.10]) 8, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MI8MPa003556 8, 27 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 13:08:22 -0500 8, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 8, 27 -- by dummy.domain.name (Postfix) with ESMTP id C3B05122DFB 8, 27 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 14:08:21 -0400 (EDT) 8, 27 -- Received: from mx2.stlawu.edu ([127.0.0.1]) 8, 27 -- by localhost (mx1.stlawu.edu [127.0.0.1]) (amavisd-new, port 10025) 8, 27 -- with ESMTP id 07853-08 for {microscopy-at-microscopy.com} ; 8, 27 -- Fri, 22 Jul 2005 14:08:21 -0400 (EDT) 8, 27 -- Received: from stlawu.edu (pflugheber-jill.stlawu.local [10.32.122.85]) 8, 27 -- by mx2.stlawu.edu (Postfix) with ESMTP id A6A79122DF2 8, 27 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 14:08:21 -0400 (EDT) 8, 27 -- Message-ID: {42E13615.4050107-at-stlawu.edu} 8, 27 -- Date: Fri, 22 Jul 2005 14:08:21 -0400 8, 27 -- From: Jill Pflugheber {jpflugheber-at-stlawu.edu} 8, 27 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 8, 27 -- X-Accept-Language: en-us, en 8, 27 -- MIME-Version: 1.0 8, 27 -- To: microscopy-at-microscopy.com 8, 27 -- Subject: Re: [Microscopy] Environmental SEM in North Texas 8, 27 -- References: {200507221727.j6MHRSI8032125-at-ns.microscopy.com} 8, 27 -- In-Reply-To: {200507221727.j6MHRSI8032125-at-ns.microscopy.com} 8, 27 -- Content-Type: text/plain; charset=us-ascii; format=flowed 8, 27 -- Content-Transfer-Encoding: 7bit 8, 27 -- X-Virus-Scanned: by amavisd-new at stlawu.edu ==============================End of - Headers==============================
I'm sure that once you replace the battery, JEOL will be able to help you over the phone. They were always very accommodating when we had a JSM 35, even when it was not under contract.
Ron L
-----Original Message----- X-from: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu] Sent: Friday, July 22, 2005 2:50 PM To: lherault-at-bu.edu
Hi:
Anybody remember how to reset the computer on a JEOL 1200EX? Its battery died and it has lost its mind.
Thanks
Jon
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
==============================Original Headers============================== 7, 17 -- From jmkrupp-at-cats.ucsc.edu Fri Jul 22 13:45:46 2005 7, 17 -- Received: from cats-mx4.ucsc.edu (cats-mx4.ucsc.edu [128.114.125.37]) 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MIjjir011931 7, 17 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 13:45:45 -0500 7, 17 -- Received: from [128.114.25.163] (dhcp-25-163.ucsc.edu [128.114.25.163]) 7, 17 -- by cats-mx4.ucsc.edu (8.13.1/8.13.1) with SMTP id j6MIhkGM009366 7, 17 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 11:43:49 -0700 (PDT) 7, 17 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu 7, 17 -- Message-Id: {v01550100bf06ee74d4cd-at-[128.114.25.223]} 7, 17 -- Mime-Version: 1.0 7, 17 -- Content-Type: text/plain; charset="us-ascii" 7, 17 -- Date: Fri, 22 Jul 2005 11:43:15 -0700 7, 17 -- To: microscopy-at-microscopy.com 7, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) 7, 17 -- Subject: reset JEOL 1200EX? 7, 17 -- X-UCSC-CATS-MailScanner: Found to be clean 7, 17 -- X-UCSC-CATS-MailScanner-SpamCheck: ==============================End of - Headers==============================
==============================Original Headers============================== 17, 22 -- From lherault-at-bu.edu Fri Jul 22 13:53:15 2005 17, 22 -- Received: from relay16-159.bu.edu (relay16-159.bu.edu [128.197.159.83]) 17, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MIrFRe019898 17, 22 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 13:53:15 -0500 17, 22 -- Received: from lherault (dhcp212-61.bumc.bu.edu [155.41.212.61]) 17, 22 -- by relay16-159.bu.edu (8.12.8/8.12.8) with SMTP id j6MIqUEX028221 17, 22 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 14:52:31 -0400 17, 22 -- From: "Ron L'Herault" {lherault-at-bu.edu} 17, 22 -- To: {microscopy-at-microscopy.com} 17, 22 -- Subject: RE: [Microscopy] reset JEOL 1200EX? 17, 22 -- Date: Fri, 22 Jul 2005 14:56:44 -0400 17, 22 -- Message-ID: {NHBBLPOLELMEEBAIFOHFAECLGEAA.lherault-at-bu.edu} 17, 22 -- MIME-Version: 1.0 17, 22 -- Content-Type: text/plain; 17, 22 -- charset="iso-8859-1" 17, 22 -- Content-Transfer-Encoding: 7bit 17, 22 -- X-Priority: 3 (Normal) 17, 22 -- X-MSMail-Priority: Normal 17, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2910.0) 17, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1441 17, 22 -- Importance: Normal 17, 22 -- In-Reply-To: {200507221849.j6MInbHp018807-at-ns.microscopy.com} ==============================End of - Headers==============================
Hi Jon, One thing that helps when we get odd characters showing up on our 1200EX display is to hit control and A simultaneously. This puts an asterisk on the top of page. Then simply type RESET then hit return. Let me know if you need a hard reboot. I can give you info on that as well. Good luck. Tom
-- Tom Januszewski Senior Electron Microscopist Molecular and Cellular Imaging Facility UT Southwestern Medical Center at Dallas Dallas, TX 75390-9039 214-648-7291 214-648-6408 (FAX) tom.januszewski-at-UTSouthwestern.edu
The book "Electron microdiffraction" by J.M. Zuo and J. Spence had such program too.
Ch
---- Original message ---- } Date: Fri, 22 Jul 2005 12:08:48 -0500 } From: walck-at-southbaytech.com } Subject: [Microscopy] RE: AskAMicroscopist: How to index high order Kikuchi lines of } To: clei-at-uiuc.edu } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 4, 26 -- From clei-at-uiuc.edu Fri Jul 22 14:58:54 2005 4, 26 -- Received: from expredir6.cites.uiuc.edu (expredir6.cites.uiuc.edu [128.174.5.97]) 4, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MJwsOK012087 4, 26 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 14:58:54 -0500 4, 26 -- Received: from expms6.cites.uiuc.edu (expms6.cites.uiuc.edu [128.174.5.43]) 4, 26 -- by expredir6.cites.uiuc.edu (8.12.11/8.12.11) with ESMTP id j6MJwoeq013378 4, 26 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 14:58:53 -0500 (CDT) 4, 26 -- Received: from expms6.cites.uiuc.edu (localhost.cites.uiuc.edu [127.0.0.1]) 4, 26 -- by expms6.cites.uiuc.edu (MOS 3.4.8-GR) 4, 26 -- with ESMTP id AVA65927; 4, 26 -- Fri, 22 Jul 2005 14:58:49 -0500 (CDT) 4, 26 -- Received: from 128.174.5.212 4, 26 -- by expms6.cites.uiuc.edu (MOS 3.4.8-GR) 4, 26 -- with HTTP/1.1; 4, 26 -- Fri, 22 Jul 2005 13:58:49 -0600 4, 26 -- Date: Fri, 22 Jul 2005 13:58:49 -0600 4, 26 -- From: Changhui LEI {clei-at-uiuc.edu} 4, 26 -- Subject: Re: [Microscopy] RE: AskAMicroscopist: How 4, 26 -- to index high order Kikuchi lines of 4, 26 -- To: microscopy-at-microscopy.com 4, 26 -- Reply-To: clei-at-uiuc.edu 4, 26 -- X-Mailer: Webmail Mirapoint Direct 3.4.8-GR 4, 26 -- MIME-Version: 1.0 4, 26 -- Message-Id: {78bde74a.2003174b.8198700-at-expms6.cites.uiuc.edu} 4, 26 -- Content-Type: text/plain; charset=us-ascii 4, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
As far as I remember, reset will erase information about pole-piece and perhaps some other information from the computer memory, so you need to write it down before resetting. I would try just to turn off-on computer first (there is switch for it if you open back panel of the right console (where computer is)). Sergey
At 12:20 PM 7/22/2005, you wrote:
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==============================Original Headers============================== 6, 25 -- From sryazant-at-ucla.edu Fri Jul 22 15:51:30 2005 6, 25 -- Received: from smtp-8.smtp.ucla.edu (smtp-8.smtp.ucla.edu [169.232.47.137]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6MKpT9j020480 6, 25 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 15:51:29 -0500 6, 25 -- Received: from mail.ucla.edu (mail.ucla.edu [169.232.47.141]) 6, 25 -- by smtp-8.smtp.ucla.edu (8.13.4/8.13.4) with ESMTP id j6MKpQ8n032223 6, 25 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 13:51:26 -0700 6, 25 -- Received: from kopoba.ucla.edu (ts11-171.dialup.bol.ucla.edu [169.232.228.181]) 6, 25 -- (authenticated bits=0) 6, 25 -- by mail.ucla.edu (8.13.4/8.13.4) with ESMTP id j6MKpGsS009642 6, 25 -- (version=TLSv1/SSLv3 cipher=DES-CBC3-SHA bits=168 verify=NOT) 6, 25 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 13:51:20 -0700 6, 25 -- Message-Id: {6.1.2.0.2.20050722134743.032c8d60-at-mail.ucla.edu} 6, 25 -- X-Sender: sryazant-at-mail.ucla.edu 6, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 25 -- Date: Fri, 22 Jul 2005 13:51:03 -0700 6, 25 -- To: microscopy-at-microscopy.com 6, 25 -- From: Sergey {sryazant-at-ucla.edu} 6, 25 -- Subject: Re: [Microscopy] Re: reset JEOL 1200EX? 6, 25 -- In-Reply-To: {200507221920.j6MJKZe4001792-at-ns.microscopy.com} 6, 25 -- References: {200507221920.j6MJKZe4001792-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 25 -- X-Probable-Spam: no 6, 25 -- X-Scanned-By: smtp.ucla.edu on 169.232.47.137 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eoptics-at-mcmaster.ca) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, July 22, 2005 at 12:43:58 ---------------------------------------------------------------------------
Email: eoptics-at-mcmaster.ca Name: Fred Pearson
Organization: McMaster University
Title-Subject: [Filtered] MListserver: Making Nickel Evaporated Films
Question: Good day
I have been trying to produce films by evaporating nickel onto a glass slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear a very thin soap film across the slide and wipe it until the almost disappears. I then evaporate the nickel. I then score the slide with a sharp needle and immerse it into distilled water at about a 30 degree angle. The film floats in sections. I then scoop up the pieces with 200 um. aluminum grids.
The problem is that when it begins to dry, the film pops within each of the grid squares and disappears.
What I am I doing wrong??? Is the film not thick enough?
I have made other types of films with this exact same procedure with no problems.
On Jul 22, 2005, at 3:36 PM, eoptics-at-mcmaster.ca wrote:
} I have been trying to produce films by evaporating nickel onto a glass } slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear } a very thin soap film across the slide and wipe it until the almost } disappears. I then evaporate the nickel. I then score the slide with a } sharp needle and immerse it into distilled water at about a 30 degree } angle. The film floats in sections. I then scoop up the pieces with } 200 um. aluminum grids. } } The problem is that when it begins to dry, the film pops within each } of the grid squares and disappears. } } What I am I doing wrong??? Is the film not thick enough? } } I have made other types of films with this exact same procedure with } no problems. } Dear Fred, If the film breaks apart as it is floated off--assuming that is what you mean by "in sections"--then it may not be structurally strong enough. You don't say how thick the film is, so I don't have a feeling whether making it thicker will solve the problem. One thing you could try is to take the wet grid and put it onto a drop of ethanol, which will lower the surface tension, and that might reduce the forces that pop the film. It may be that the evaporated nickel is too crystalline with relatively weak cohesion between crystals, making it weaker than might be expected. A possible alternative is to put a formvar coat onto the grids, evaporate the Ni onto the formvar, then dissolve the formvar away with CHCl3. If you try this, be careful not to let pools of CHCl3 touch the grids, but put them on a piece of filter paper uphill from the CHCl3 and let only the liquid drawn up to the grids through the paper touch the formvar--the same technique for dissolving formvar from under a layer of C. Good luck. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 26 -- From tivol-at-caltech.edu Fri Jul 22 19:36:16 2005 5, 26 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6N0aFLY006137 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 19:36:15 -0500 5, 26 -- Received: from earth-dog (earth-dog [192.168.1.3]) 5, 26 -- by earth-ox-postvirus (Postfix) with ESMTP id 41B2E109A5E 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 17:36:15 -0700 (PDT) 5, 26 -- Received: from fire-ox ([192.168.1.31]) 5, 26 -- by earth-dog (MailMonitor for SMTP v1.2.2 ) ; 5, 26 -- Fri, 22 Jul 2005 17:36:14 -0700 (PDT) 5, 26 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 5, 26 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 7C66A35271 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jul 2005 17:36:14 -0700 (PDT) 5, 26 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 26 -- In-Reply-To: {200507222236.j6MMaJ4X029783-at-ns.microscopy.com} 5, 26 -- References: {200507222236.j6MMaJ4X029783-at-ns.microscopy.com} 5, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 26 -- Message-Id: {b4d43d1acf7b1588cbbe9272289c496e-at-caltech.edu} 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 26 -- Subject: Re: [Microscopy] viaWWW: Making Nickel Evaporated Films 5, 26 -- Date: Fri, 22 Jul 2005 17:37:03 -0700 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- X-Mailer: Apple Mail (2.622) 5, 26 -- X-Spam-Status: No, hits=0.0 tagged_above=-100000.0 required=5.0 5, 26 -- X-Spam-Level: ==============================End of - Headers==============================
Fred Pearson wrote: ==================================================== I have been trying to produce films by evaporating nickel onto a glass slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear a very thin soap film across the slide and wipe it until the almost disappears. I then evaporate the nickel. I then score the slide with a sharp needle and immerse it into distilled water at about a 30 degree angle. The film floats in sections. I then scoop up the pieces with 200 um. aluminum grids.
The problem is that when it begins to dry, the film pops within each of the grid squares and disappears.
What I am I doing wrong??? Is the film not thick enough?
I have made other types of films with this exact same procedure with no problems. =================================================== You did not mention how you were applying the Ni films, if you are trying to do this by sputtering, you would need a truly clean and UHV environment or else, so I have been led to believe, you will end up with a significant amount of oxide.
My biggest concern would be with the smearing of the "very thin soap film across the slide". What looks "disappeared" to the eye could be big globs so far as TEM observation dimensions are concerned (if transferred to the film) and that could lead to film instability in the electron beam.
An alternative approach might be to evaporate Victawet® (if you are not familiar with this interesting surfactant, see URL http://www.2spi.com/catalog/spec_prep/evapor_3c.shtml
By the vacuum evaporation of Victawet, you can apply a more thinner and more uniform layer than you could ever get by "wiping" and experience has been that whatever Victawet that might be left (it will dissolve in water) won't be sensitive in the presence of the electron beam.
We have ourselves not tried Victawet with Ni but have used it quite successfully with other coating materials and it really does work.
Disclaimer: SPI Supplies is a supplier of Victawet for electron microscopy so we would have a vested interest in having more people using Victawet.
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 19, 27 -- From cgarber-at-2spi.com Sat Jul 23 13:36:45 2005 19, 27 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 19, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6NIajZb021735 19, 27 -- for {microscopy-at-microscopy.com} ; Sat, 23 Jul 2005 13:36:45 -0500 19, 27 -- Received: from ibm1x23g2abfyg ([12.167.162.5]) 19, 27 -- (authenticated bits=0) 19, 27 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id j6NIaiA6000992; 19, 27 -- Sat, 23 Jul 2005 14:36:44 -0400 19, 27 -- X-IDV-FirstRcvd: [12.167.162.5] 19, 27 -- X-IDV-HELO: ibm1x23g2abfyg 19, 27 -- X-IDV-Authenticated-User: cgarber 19, 27 -- Message-ID: {067101c58fb5$7a48a180$05a2a70c-at-ibm1x23g2abfyg} 19, 27 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 19, 27 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 19, 27 -- To: {microscopy-at-microscopy.com} 19, 27 -- Subject: Making Ni films 19, 27 -- Date: Sat, 23 Jul 2005 14:36:39 -0400 19, 27 -- MIME-Version: 1.0 19, 27 -- Content-Type: text/plain; 19, 27 -- format=flowed; 19, 27 -- charset="Windows-1252"; 19, 27 -- reply-type=original 19, 27 -- Content-Transfer-Encoding: 8bit 19, 27 -- X-Priority: 3 19, 27 -- X-MSMail-Priority: Normal 19, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 19, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
it is quite easy to perform a full reset on a 1200 II. You will however lose a lot of information, for example type of vacuum system, type of polepiece and other serious data that the microscope needs to operate correctly, I would suggest you contact your local Jeol office who can give instructions over the phone,
Richard Hey
Principal Technical Support Engineer Jeol (UK) Ltd.
The views expressed herein do not reflect the views of Jeol
At 15:56 22/07/2005 -0500, you wrote:
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==============================Original Headers============================== 37, 18 -- From richard-at-torland.demon.co.uk Sun Jul 24 06:07:04 2005 37, 18 -- Received: from anchor-post-35.mail.demon.net (anchor-post-35.mail.demon.net [194.217.242.85]) 37, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6OB73AK021643 37, 18 -- for {microscopy-at-microscopy.com} ; Sun, 24 Jul 2005 06:07:03 -0500 37, 18 -- Received: from torland.demon.co.uk ([80.177.208.173] helo=lancelot.torland.demon.co.uk) 37, 18 -- by anchor-post-35.mail.demon.net with esmtp (Exim 4.42) 37, 18 -- id 1DweFb-0006le-JL 37, 18 -- for microscopy-at-microscopy.com; Sun, 24 Jul 2005 11:02:32 +0000 37, 18 -- Message-Id: {5.2.1.1.2.20050724120628.01ac5e10-at-pop3.demon.co.uk} 37, 18 -- X-Sender: torland-at-pop3.demon.co.uk 37, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 37, 18 -- Date: Sun, 24 Jul 2005 12:10:06 +0100 37, 18 -- To: microscopy-at-microscopy.com 37, 18 -- From: Richard {richard-at-torland.demon.co.uk} 37, 18 -- Subject: Re: [Microscopy] reset JEOL 1200EX? 37, 18 -- In-Reply-To: {200507222056.j6MKu621027004-at-ns.microscopy.com} 37, 18 -- Mime-Version: 1.0 37, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (philf-at-newton.umsl.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, July 23, 2005 at 14:43:34 ---------------------------------------------------------------------------
Email: philf-at-newton.umsl.edu Name: Phil Fraundorf
We're developing a webpage* for simulation and analysis of imaging, diffraction, and spectroscopic data inspired by the fact that for small numbers of atoms these effects are easier to model. One can already use it to index cross-fringe lattice images and single-crystal diffraction patterns against some candidate structures, and to generate projected-potential and truncated Debye-sum diffraction images down any orientation. The latter allow one to quantify finite-crystal effects, e.g. of reduced specimen thickness in directions perpendicular to a g-vector. This gives rise to high-frequency "fore-shortening tails" in powder patterns, even as reduced-thickness parallel to the same g-vector broadens diffraction spots symmetrically. These effects are quite important in the study, for example, of data from graphene nano-structures**.
As far as education is concerned, this virtual goniometer will offer your students a chance to take and ponder data from unknown nanostructures, without the cost of scope time. Moreover, for those into diffraction, specimen-tilting while viewing from the side also nicely illustrates a flat Ewald-sphere in action.
Suggestions and corrections (we're just getting started) to philf-at-newton.umsl.edu are invited. Except for this announcement, the system is too much in its infancy to warrant much bandwidth on the listserver. However, you might want to bookmark it, as we hope to develop it into a reliable resource for research and teaching in days ahead.
Ok, so this is not serious journalism but it was in the popular press - did anyone else read Parade columnist Marilyn vos Savant's article last Sunday "Are men smarter than women?"? The article is mainly about women in science or the lack thereof. About half way through the article she decides to bash microscopists:
"And note that dictators - who aren't any stronger than other men - are never women. Maybe females just don't have whatever it takes to bulldoze their way to this dubious sort of "success". No one thinks the paucity of women in the field of ruthless domination is because they aren't smart enough! So why should anyone be shocked to find that most bright people - including women - would flee from the sight of a microscope?!"
Marilyn is in the Guinness Book of Records Hall of Fame for having the highest IQ ever recorded. If you feel so inclined to enlighten Marilyn about microscopy (or bad analogies) you can email her at {marilyn-at-parade.com} .
see you "not so bright people" in Honolulu, Beth
PS - she is intelligent enough to say that neither gender is smarter than the other.
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
well this is a good 2 minutes of my life i will never get back, trume there haven't benn any women dictators, they just attach themselves to ditators.
--- beth-at-plantbio.uga.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Ok, so this is not serious journalism but it was in } the popular press - } did anyone else read Parade columnist Marilyn vos } Savant's article last } Sunday "Are men smarter than women?"? The article is } mainly about women } in science or the lack thereof. About half way } through the article she } decides to bash microscopists: } } "And note that dictators - who aren't any stronger } than other men - are } never women. Maybe females just don't have whatever } it takes to } bulldoze their way to this dubious sort of } "success". No one thinks the } paucity of women in the field of ruthless domination } is because they } aren't smart enough! So why should anyone be shocked } to find that most } bright people - including women - would flee from } the sight of a } microscope?!" } } Marilyn is in the Guinness Book of Records Hall of } Fame for having the } highest IQ ever recorded. If you feel so inclined to } enlighten Marilyn } about microscopy (or bad analogies) you can email } her at } {marilyn-at-parade.com} . } } see you "not so bright people" in Honolulu, } Beth } } PS - she is intelligent enough to say that neither } gender is smarter } than the other. } } ********************************************************************** } Beth Richardson } EM Lab Coordinator } Plant Biology Department } University of Georgia } Athens, GA 30602-7271 } } Phone - (706) 542-1790 & FAX - (706) 542-1805 } http://www.plantbio.uga.edu/emlab } } "Between the two evils, } I always pick the one I never tried before". Mae } West (1893-1980) } ******************************************************************* } } "And it's only the giving that makes you what you } are". } Wond'ring Aloud, Jethro Tull (Aqualung) } } ************************************************************************ } } *** } } } } ==============================Original } Headers============================== } 12, 18 -- From beth-at-plantbio.uga.edu Mon Jul 25 } 10:29:34 2005 } 12, 18 -- Received: from dogwood.plantbio.uga.edu } (dogwood.plantbio.uga.edu [128.192.26.2]) } 12, 18 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6PFTXIa021865 } 12, 18 -- for {microscopy-at-microscopy.com} ; Mon, 25 } Jul 2005 10:29:33 -0500 } 12, 18 -- Received: from localhost ([127.0.0.1]) } 12, 18 -- by dogwood.plantbio.uga.edu } 12, 18 -- (using TLSv1/SSLv3 with cipher } DES-CBC3-SHA (168 bits)) } 12, 18 -- for microscopy-at-microscopy.com; } 12, 18 -- Mon, 25 Jul 2005 11:29:29 -0400 } 12, 18 -- Date: Mon, 25 Jul 2005 11:29:30 -0400 } 12, 18 -- Mime-Version: 1.0 (Apple Message framework } v553) } 12, 18 -- Content-Type: text/plain; delsp=yes; } charset=US-ASCII; format=flowed } 12, 18 -- Subject: "most bright people" } 12, 18 -- From: Beth Richardson } {beth-at-plantbio.uga.edu} } 12, 18 -- To: microscopy {microscopy-at-microscopy.com} } 12, 18 -- Content-Transfer-Encoding: 7bit } 12, 18 -- Message-Id: } {E4EE6FFB-FD20-11D9-8C24-000393137C00-at-plantbio.uga.edu} } 12, 18 -- X-Mailer: Apple Mail (2.553) } ==============================End of - } Headers============================== }
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====== A student working on a Geoarchaeology masters dissertation writes:
I would like to use the environmental SEM soon after the centre re-opens on the 8th August. I have potentially up to 30 soil and sediment samples that I am hoping consist of loess to some degree or other. My plan is to examine the quartz grains in the samples for evidence of frost-shattering patterns. I've never used this kind of equipment before and I would very much appreciate any advice you may have regarding preparing these samples for viewing. Sample size, condition, mounting etc.
====== Thanks for your time,
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
==============================Original Headers============================== 8, 21 -- From hinmeigeng-at-hotmail.com Mon Jul 25 15:43:59 2005 8, 21 -- Received: from hotmail.com (bay101-f30.bay101.hotmail.com [64.4.56.40]) 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6PKhx61009344 8, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 25 Jul 2005 15:43:59 -0500 8, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 8, 21 -- Mon, 25 Jul 2005 13:43:58 -0700 8, 21 -- Message-ID: {BAY101-F30CFFD9E2F324B51359788CACA0-at-phx.gbl} 8, 21 -- Received: from 64.4.56.204 by by101fd.bay101.hotmail.msn.com with HTTP; 8, 21 -- Mon, 25 Jul 2005 20:43:57 GMT 8, 21 -- X-Originating-IP: [64.4.56.204] 8, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 8, 21 -- X-Sender: hinmeigeng-at-hotmail.com 8, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk 8, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 8, 21 -- To: Microscopy-at-MSA.Microscopy.Com 8, 21 -- Cc: R.H.Olley-at-reading.ac.uk 8, 21 -- Subject: ESEM of soil 8, 21 -- Date: Mon, 25 Jul 2005 20:43:57 +0000 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; format=flowed 8, 21 -- X-OriginalArrivalTime: 25 Jul 2005 20:43:58.0068 (UTC) FILETIME=[94929B40:01C59159] ==============================End of - Headers==============================
Hi and thanks to all of you who responded to my question about gummy blocks. Here is a summary of the suggestions you sent:
-extra changes of absolute ethanol -lower temp, longer cure -more gradations of ethanol-epon mix leading up to pure resin, with agitation at all steps -use fresh accelerator ( {6 months old) and switch to BDMA instead of DMP-30 -use HPMA (Hydroypropyl Methacrylate) in place of PO as a final dehydrating agent, and then stepwise into Epon (it won't eat the plastic dishes) -use polypropylene culture dishes -remove the cover glass and embed in Chang mold
The last 2 suggestions won't work for me. The dishes come in just 1 "flavor" and if I could remove the cover glass without destroying it, I would have. These are attached with Sylgard (I think), and its a tight bond.
Thanks again, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
==============================Original Headers============================== 4, 20 -- From lcgould-at-med.cornell.edu Mon Jul 25 15:52:01 2005 4, 20 -- Received: from smtp-in1.med.cornell.edu (smtp-in1.med.cornell.edu [140.251.1.25]) 4, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6PKq1re017089 4, 20 -- for {microscopy-at-msa.microscopy.com} ; Mon, 25 Jul 2005 15:52:01 -0500 4, 20 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 4, 20 -- by smtp-in1.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6PKpwed271390 4, 20 -- for {microscopy-at-msa.microscopy.com} ; Mon, 25 Jul 2005 16:51:58 -0400 4, 20 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu 4, 20 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 4, 20 -- with ESMTP id {0IK7001JHBAKIC70-at-mpx1.med.cornell.edu} for 4, 20 -- microscopy-at-msa.microscopy.com; Mon, 25 Jul 2005 16:51:57 -0400 (EDT) 4, 20 -- Date: Mon, 25 Jul 2005 16:51:55 -0400 4, 20 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 4, 20 -- Subject: gummy blocks-summary of responses 4, 20 -- X-Sender: lcgould-at-pop.med.cornell.edu 4, 20 -- To: "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 4, 20 -- Message-id: {p06020409bf0afd87b3d9-at-[140.251.145.131]} 4, 20 -- MIME-version: 1.0 4, 20 -- Content-type: text/plain; charset=us-ascii; format=flowed 4, 20 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.7.25.24 ==============================End of - Headers==============================
Isn't this the same Marilyn vos Savant who wrote in her column that a bullet fired from a high powered gun into the air was harmless when it came back down? I just want to know who did the IQ testing.
John Mardinly Intel
The opinion of this author is not necessarily the opinion of Intel corp.
-----Original Message----- X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu] Sent: Monday, July 25, 2005 8:30 AM To: Mardinly, John
Ok, so this is not serious journalism but it was in the popular press -
did anyone else read Parade columnist Marilyn vos Savant's article last
Sunday "Are men smarter than women?"? The article is mainly about women
in science or the lack thereof. About half way through the article she decides to bash microscopists:
"And note that dictators - who aren't any stronger than other men - are
never women. Maybe females just don't have whatever it takes to bulldoze their way to this dubious sort of "success". No one thinks the
paucity of women in the field of ruthless domination is because they aren't smart enough! So why should anyone be shocked to find that most bright people - including women - would flee from the sight of a microscope?!"
Marilyn is in the Guinness Book of Records Hall of Fame for having the highest IQ ever recorded. If you feel so inclined to enlighten Marilyn about microscopy (or bad analogies) you can email her at {marilyn-at-parade.com} .
see you "not so bright people" in Honolulu, Beth
PS - she is intelligent enough to say that neither gender is smarter than the other.
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
Well who knows. Perhaps Marilyn has a point about the lack of female dictators through history. But that's not to say that women haven't caused just as much damage. Take Helen of Troy for example. (Joke)
Since when was there ever a positive correlation between so-called intelligence and the abuse of opportunistic bigotry? Marilyn's mission is to help her employer's media company make money. Thus for the good of all this sort of crap journalism should just be ignored.
Anyway, aren't IQ tests supposed to be culture and gender biased in favor of white western males? Has anyone seen a photo of "Marilyn"?
} } "And note that dictators - who aren't any stronger than other men - are } } never women. Maybe females just don't have whatever it takes to } bulldoze their way to this dubious sort of "success". No one thinks the } } paucity of women in the field of ruthless domination is because they } aren't smart enough! So why should anyone be shocked to find that most } bright people - including women - would flee from the sight of a } microscope?!" } } Marilyn is in the Guinness Book of Records Hall of Fame for having the } highest IQ ever recorded. If you feel so inclined to enlighten Marilyn } about microscopy (or bad analogies) you can email her at } {marilyn-at-parade.com} .
==============================Original Headers============================== 7, 26 -- From ard-at-ansto.gov.au Mon Jul 25 18:37:23 2005 7, 26 -- Received: from tachyon.gw.ansto.gov.au (tachyon.gw.ansto.gov.au [137.157.8.253]) 7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6PNbMwO001736 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 25 Jul 2005 18:37:23 -0500 7, 26 -- Received: (from uucp-at-localhost) 7, 26 -- by tachyon.gw.ansto.gov.au (8.11.7p1+Sun/8.11.7) id j6PNbLn10794 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:37:21 +1000 (EST) 7, 26 -- Received: from jenner.ansto.gov.au(137.157.59.25) by tachyon.gw.ansto.gov.au via csmap (V6.0) 7, 26 -- id srcAAAwSaifv; Tue, 26 Jul 05 09:37:20 +1000 7, 26 -- Received: from hadron.ansto.gov.au (hadron.ansto.gov.au [137.157.13.219]) 7, 26 -- by jenner.ansto.gov.au (8.12.10/8.12.10) with ESMTP id j6PNbKBJ032450 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:37:20 +1000 7, 26 -- Received: from [137.157.95.82] (arthur.amat.ansto.gov.au [137.157.95.82]) 7, 26 -- by hadron.ansto.gov.au (8.9.3/8.9.3) with ESMTP id JAA15758 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:37:18 +1000 (EST) 7, 26 -- Mime-Version: 1.0 7, 26 -- X-Sender: ard-at-hadron.ansto.gov.au 7, 26 -- Message-Id: {v04210100bf0b1e868643-at-[137.157.95.82]} 7, 26 -- In-Reply-To: {200507252229.j6PMTacc027945-at-ns.microscopy.com} 7, 26 -- References: {200507252229.j6PMTacc027945-at-ns.microscopy.com} 7, 26 -- Date: Tue, 26 Jul 2005 09:37:17 +1000 7, 26 -- To: microscopy-at-microscopy.com 7, 26 -- From: Arthur Day {ard-at-ansto.gov.au} 7, 26 -- Subject: [Microscopy] RE: "most bright people" 7, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 26 -- X-ANSTO-MailScanner: Found to be clean ==============================End of - Headers==============================
women throughout history have latched themselves on to men od power, must i mention eva braum. i am not certain as to why beth would even bring this up, as far as i can see Marilyn's form of journalism is self serving crap. i am not even convinced the letters wrtten to her are real. so i ask beth, what was your motivation?
--- ard-at-ansto.gov.au wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Well who knows. Perhaps Marilyn has a point about } the lack of female } dictators through history. But that's not to say } that women haven't } caused just as much damage. Take Helen of Troy for } example. (Joke) } } Since when was there ever a positive correlation } between so-called } intelligence and the abuse of opportunistic bigotry? } Marilyn's } mission is to help her employer's media company make } money. Thus for } the good of all this sort of crap journalism should } just be ignored. } } Anyway, aren't IQ tests supposed to be culture and } gender biased in } favor of white western males? Has anyone seen a } photo of "Marilyn"? } } } } } } "And note that dictators - who aren't any stronger } than other men - are } } } } never women. Maybe females just don't have whatever } it takes to } } bulldoze their way to this dubious sort of } "success". No one thinks the } } } } paucity of women in the field of ruthless } domination is because they } } aren't smart enough! So why should anyone be } shocked to find that most } } bright people - including women - would flee from } the sight of a } } microscope?!" } } } } Marilyn is in the Guinness Book of Records Hall of } Fame for having the } } highest IQ ever recorded. If you feel so inclined } to enlighten Marilyn } } about microscopy (or bad analogies) you can email } her at } } {marilyn-at-parade.com} . } } } ==============================Original } Headers============================== } 7, 26 -- From ard-at-ansto.gov.au Mon Jul 25 18:37:23 } 2005 } 7, 26 -- Received: from tachyon.gw.ansto.gov.au } (tachyon.gw.ansto.gov.au [137.157.8.253]) } 7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j6PNbMwO001736 } 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 25 } Jul 2005 18:37:23 -0500 } 7, 26 -- Received: (from uucp-at-localhost) } 7, 26 -- by tachyon.gw.ansto.gov.au } (8.11.7p1+Sun/8.11.7) id j6PNbLn10794 } 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 } Jul 2005 09:37:21 +1000 (EST) } 7, 26 -- Received: from } jenner.ansto.gov.au(137.157.59.25) by } tachyon.gw.ansto.gov.au via csmap (V6.0) } 7, 26 -- id srcAAAwSaifv; Tue, 26 Jul 05 09:37:20 } +1000 } 7, 26 -- Received: from hadron.ansto.gov.au } (hadron.ansto.gov.au [137.157.13.219]) } 7, 26 -- by jenner.ansto.gov.au (8.12.10/8.12.10) } with ESMTP id j6PNbKBJ032450 } 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 } Jul 2005 09:37:20 +1000 } 7, 26 -- Received: from [137.157.95.82] } (arthur.amat.ansto.gov.au [137.157.95.82]) } 7, 26 -- by hadron.ansto.gov.au (8.9.3/8.9.3) with } ESMTP id JAA15758 } 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 } Jul 2005 09:37:18 +1000 (EST) } 7, 26 -- Mime-Version: 1.0 } 7, 26 -- X-Sender: ard-at-hadron.ansto.gov.au } 7, 26 -- Message-Id: } {v04210100bf0b1e868643-at-[137.157.95.82]} } 7, 26 -- In-Reply-To: } {200507252229.j6PMTacc027945-at-ns.microscopy.com} } 7, 26 -- References: } {200507252229.j6PMTacc027945-at-ns.microscopy.com} } 7, 26 -- Date: Tue, 26 Jul 2005 09:37:17 +1000 } 7, 26 -- To: microscopy-at-microscopy.com } 7, 26 -- From: Arthur Day {ard-at-ansto.gov.au} } 7, 26 -- Subject: [Microscopy] RE: "most bright } people" } 7, 26 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } 7, 26 -- X-ANSTO-MailScanner: Found to be clean } ==============================End of - } Headers============================== }
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My guess is that Beth thought this was pretty funny and wanted to share it with her friends and colleagues who she is really looking forward to seeing next week. If that's the case, I must agree.
Just a guess. I'm looking forward to meeting you Beth!
All best,
Angela } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } women throughout history have latched themselves on to } men od power, must i mention eva braum. } i am not certain as to why beth would even bring this } up, as far as i can see Marilyn's form of journalism } is self serving crap. i am not even convinced the } letters wrtten to her are real. } so i ask beth, what was your motivation? } } --- ard-at-ansto.gov.au wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Well who knows. Perhaps Marilyn has a point about } } the lack of female } } dictators through history. But that's not to say } } that women haven't } } caused just as much damage. Take Helen of Troy for } } example. (Joke) } } } } Since when was there ever a positive correlation } } between so-called } } intelligence and the abuse of opportunistic bigotry? } } Marilyn's } } mission is to help her employer's media company make } } money. Thus for } } the good of all this sort of crap journalism should } } just be ignored. } } } } Anyway, aren't IQ tests supposed to be culture and } } gender biased in } } favor of white western males? Has anyone seen a } } photo of "Marilyn"? } } } } } } } } } } "And note that dictators - who aren't any stronger } } than other men - are } } } } } } never women. Maybe females just don't have whatever } } it takes to } } } bulldoze their way to this dubious sort of } } "success". No one thinks the } } } } } } paucity of women in the field of ruthless } } domination is because they } } } aren't smart enough! So why should anyone be } } shocked to find that most } } } bright people - including women - would flee from } } the sight of a } } } microscope?!" } } } } } } Marilyn is in the Guinness Book of Records Hall of } } Fame for having the } } } highest IQ ever recorded. If you feel so inclined } } to enlighten Marilyn } } } about microscopy (or bad analogies) you can email } } her at } } } {marilyn-at-parade.com} . } } } } } } ==============================Original } } Headers============================== } } 7, 26 -- From ard-at-ansto.gov.au Mon Jul 25 18:37:23 } } 2005 } } 7, 26 -- Received: from tachyon.gw.ansto.gov.au } } (tachyon.gw.ansto.gov.au [137.157.8.253]) } } 7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with } } ESMTP id j6PNbMwO001736 } } 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 25 } } Jul 2005 18:37:23 -0500 } } 7, 26 -- Received: (from uucp-at-localhost) } } 7, 26 -- by tachyon.gw.ansto.gov.au } } (8.11.7p1+Sun/8.11.7) id j6PNbLn10794 } } 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 } } Jul 2005 09:37:21 +1000 (EST) } } 7, 26 -- Received: from } } jenner.ansto.gov.au(137.157.59.25) by } } tachyon.gw.ansto.gov.au via csmap (V6.0) } } 7, 26 -- id srcAAAwSaifv; Tue, 26 Jul 05 09:37:20 } } +1000 } } 7, 26 -- Received: from hadron.ansto.gov.au } } (hadron.ansto.gov.au [137.157.13.219]) } } 7, 26 -- by jenner.ansto.gov.au (8.12.10/8.12.10) } } with ESMTP id j6PNbKBJ032450 } } 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 } } Jul 2005 09:37:20 +1000 } } 7, 26 -- Received: from [137.157.95.82] } } (arthur.amat.ansto.gov.au [137.157.95.82]) } } 7, 26 -- by hadron.ansto.gov.au (8.9.3/8.9.3) with } } ESMTP id JAA15758 } } 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 } } Jul 2005 09:37:18 +1000 (EST) } } 7, 26 -- Mime-Version: 1.0 } } 7, 26 -- X-Sender: ard-at-hadron.ansto.gov.au } } 7, 26 -- Message-Id: } } {v04210100bf0b1e868643-at-[137.157.95.82]} } } 7, 26 -- In-Reply-To: } } {200507252229.j6PMTacc027945-at-ns.microscopy.com} } } 7, 26 -- References: } } {200507252229.j6PMTacc027945-at-ns.microscopy.com} } } 7, 26 -- Date: Tue, 26 Jul 2005 09:37:17 +1000 } } 7, 26 -- To: microscopy-at-microscopy.com } } 7, 26 -- From: Arthur Day {ard-at-ansto.gov.au} } } 7, 26 -- Subject: [Microscopy] RE: "most bright } } people" } } 7, 26 -- Content-Type: text/plain; } } charset="us-ascii" ; format="flowed" } } 7, 26 -- X-ANSTO-MailScanner: Found to be clean } } ==============================End of - } } Headers============================== } } } } } __________________________________________________ } Do You Yahoo!? } Tired of spam? Yahoo! Mail has the best spam protection around } http://mail.yahoo.com } } ==============================Original } Headers============================== } 5, 19 -- From hoffpajo-at-yahoo.com Mon Jul 25 19:27:32 2005 } 5, 19 -- Received: from web50208.mail.yahoo.com (web50208.mail.yahoo.com } [206.190.38.49]) } 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id } j6Q0RWNH010001 } 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 25 Jul 2005 19:27:32 -0500 } 5, 19 -- Received: (qmail 4778 invoked by uid 60001); 26 Jul 2005 00:27:31 } -0000 } 5, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 5, 19 -- s=s1024; d=yahoo.com; } 5, 19 -- } h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; } 5, 19 -- } b=WcW64J93yVV6WL08OmWcRg0mDFUFv8BJDrv5w++yM1VW2Z04EKRwyHCJ0YcFroJBpVISeyb3v7sLHEnQyMSDTcoXpa6l6nQk8/s2/ikOfP1vio8/sRaB9ACvijDqSv85Wdb5LFZL9nmZwmLIzy4HstMqkvFNH0J7QNBch3yq8SU= } ; } 5, 19 -- Message-ID: {20050726002731.4776.qmail-at-web50208.mail.yahoo.com} } 5, 19 -- Received: from [68.32.53.160] by web50208.mail.yahoo.com via } HTTP; Mon, 25 Jul 2005 17:27:31 PDT } 5, 19 -- Date: Mon, 25 Jul 2005 17:27:31 -0700 (PDT) } 5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com} } 5, 19 -- Subject: Re: [Microscopy] RE: "most bright people" } 5, 19 -- To: microscopy-at-microscopy.com } 5, 19 -- In-Reply-To: {200507252339.j6PNdQEQ004369-at-ns.microscopy.com} } 5, 19 -- MIME-Version: 1.0 } 5, 19 -- Content-Type: text/plain; charset=iso-8859-1 } 5, 19 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers============================== }
-- Angela V. Klaus, Ph.D. Director, Microscopy and Imaging Facility American Museum of Natural History Central Park West and 79th Street New York, NY 10024 USA Email: avklaus-at-amnh.org Tel: 212-796-5977 Fax: 212-496-3480
==============================Original Headers============================== 7, 32 -- From avklaus-at-amnh.org Mon Jul 25 19:39:22 2005 7, 32 -- Received: from lepore.amnh.org (lepore.amnh.org [216.73.241.12]) 7, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6Q0dMhT023604 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 25 Jul 2005 19:39:22 -0500 7, 32 -- Received: from localhost (localhost [127.0.0.1]) 7, 32 -- by lepore.amnh.org (Postfix) with ESMTP id 2C3115B766 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 25 Jul 2005 20:39:22 -0400 (EDT) 7, 32 -- Received: from lepore.amnh.org ([127.0.0.1]) 7, 32 -- by localhost (escada.amnh.org [127.0.0.1]) (amavisd-new, port 10024) 7, 32 -- with ESMTP id 07694-09; Mon, 25 Jul 2005 20:39:18 -0400 (EDT) 7, 32 -- Received: from webmail.amnh.org (abel.amnh.org [216.73.241.18]) 7, 32 -- by lepore.amnh.org (Postfix) with ESMTP id 0FF485B763; 7, 32 -- Mon, 25 Jul 2005 20:39:18 -0400 (EDT) 7, 32 -- Received: from 216.226.38.2 7, 32 -- (SquirrelMail authenticated user avklaus) 7, 32 -- by webmail.amnh.org with HTTP; 7, 32 -- Mon, 25 Jul 2005 20:39:18 -0400 (EDT) 7, 32 -- Message-ID: {61211.216.226.38.2.1122338358.squirrel-at-webmail.amnh.org} 7, 32 -- In-Reply-To: {200507260027.j6Q0Rawa010072-at-ns.microscopy.com} 7, 32 -- References: {200507260027.j6Q0Rawa010072-at-ns.microscopy.com} 7, 32 -- Date: Mon, 25 Jul 2005 20:39:18 -0400 (EDT) 7, 32 -- Subject: Re: [Microscopy] Re: RE: "most bright people" 7, 32 -- From: "Angela V. Klaus" {avklaus-at-amnh.org} 7, 32 -- To: microscopy-at-microscopy.com 7, 32 -- Cc: avklaus-at-amnh.org 7, 32 -- User-Agent: SquirrelMail/1.4.4 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Type: text/plain;charset=iso-8859-1 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-Priority: 3 (Normal) 7, 32 -- Importance: Normal 7, 32 -- X-Virus-Scanned: amavisd-new at amnh.org ==============================End of - Headers==============================
Ahhh... well, then I suppose that we won't see her at M&M 2005. That is too bad. It would be great to get her enlightened input on EBSD, CL, EDS, TEM, STEM, DIC, PH, BF, DF, and other EM/LM issues/topics.
I don't see a strong (or any) correlation between IQ and microscopy. If one is in microscopy, I assume that this is because they love it. Passion is not IQ. Focus is not IQ. Some are for a job. But then too, they do it. And if poorly done, they are done.
OK. So I'm passionate about EM. Get Marilyn here at M&M 2005 and let's talk about this topic. Otherwise, relegate her to history. There are way too many other important topics to worry about then her quantitative IQ.
Regardless of my personal IQ (Intelligence Quotient), my Interest Quotient (IQ2) for EM is very high. So I would go face-to-face with her on EM. That would be fun.
gary g.
At 08:34 AM 7/25/2005, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Steve Hope all is well over there. Great excitement here in SEM land - we have just got our new Emitech K575X Sputter Coater set up. Allan suggested I email and see if you had any brilliant idea as to what kind of test specimen I could use to try it out. You may also have some advice about how to use properly? Am still enjoying the Protrain course - getting there slowly! Thanks for your help Kind regards Liz PS Allan said you're coming down here next July - looking forward to it! --
------------------------------------------------------------------------------------------------------------------------ Liz Girvan Electron Microscopist
Otago Centre for Electron Microscopy C/- Department of Anatomy and Structural Biology Otago School of Medical Sciences PO Box 913 Dunedin New Zealand Phone (03) 479 7386
http://ocem.otago.ac.nz/
**Ride the Lightning**
==============================Original Headers============================== 5, 20 -- From liz.girvan-at-stonebow.otago.ac.nz Mon Jul 25 23:41:18 2005 5, 20 -- Received: from mailhub1.otago.ac.nz (mailhub1.otago.ac.nz [139.80.64.218]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6Q4fHrF011012 5, 20 -- for {microscopy-at-microscopy.com} ; Mon, 25 Jul 2005 23:41:18 -0500 5, 20 -- Received: from galadriel.otago.ac.nz (galadriel.otago.ac.nz [139.80.64.213]) 5, 20 -- by mailhub1.otago.ac.nz (8.12.11/8.12.11) with ESMTP id j6Q4fDUb026939 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:41:13 +1200 5, 20 -- Received: from [139.80.34.127] (ou034127.otago.ac.nz [139.80.34.127]) 5, 20 -- by galadriel.otago.ac.nz (8.12.8/8.12.8) with ESMTP id j6Q4fBeg007946 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:41:13 +1200 (NZST) 5, 20 -- Mime-Version: 1.0 5, 20 -- X-Sender: st021780-at-brandywine.otago.ac.nz 5, 20 -- Message-Id: {a06110408bf0b6eb511e3-at-[139.80.34.127]} 5, 20 -- In-Reply-To: {200507141712.j6EHC4LK029548-at-ns.microscopy.com} 5, 20 -- References: {200507141712.j6EHC4LK029548-at-ns.microscopy.com} 5, 20 -- Date: Tue, 26 Jul 2005 16:41:07 +1200 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- From: Liz Girvan {liz.girvan-at-stonebow.otago.ac.nz} 5, 20 -- Subject: Sputter Coater help for Dunedin! 5, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Hi, Robert, For the beginning you can put a piece of your sample on the standard SEM metal stub (aroung 12 mm diameter) coated with carbon tape (conductive carbon glue - also standard for SEM) and use Low Vacuum mode for studying the morphology of your samples/sediments. When looking at the micro structural features of the samples and seeing something like grains, which you can attribute to quartz or can not, you start EDS acquisitions for measuring their composition. And so on and so on. First, you will find proper LV conditions for observation (HT, pressure and spot size) with Gaseous SE detector. Then, in order to separate heavy and light elements containing features you can use BSE detector. And then you can measure the composition with EDS. If you want to go up to the quantification, you have to acquire spectra from the same region at two different pressure values, because only in this way you will be able extract a right chemical information. Good Luck, Best, Inna
Dr. Inna Popov Head of The Unit for Nanoscopic Characterization The Hebrew University of Jerusalem E.Safra Campus Givat Ram Jerusalem 91904 ISRAEL www.nanoscience.huji.ac.il/unit Tel: +972 2 6584808 Fax: +972 2 6584809 email: innap-at-savion.huji.ac.il
-----Original Message----- X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com] Sent: Monday, July 25, 2005 11:47 PM To: Inna Popov
Dear Listers,
Any advice on the following?
====== A student working on a Geoarchaeology masters dissertation writes:
I would like to use the environmental SEM soon after the centre re-opens on the 8th August. I have potentially up to 30 soil and sediment samples that I am hoping consist of loess to some degree or other. My plan is to examine the quartz grains in the samples for evidence of frost-shattering patterns. I've never used this kind of equipment before and I would very much appreciate any advice you may have regarding preparing these samples for viewing. Sample size, condition, mounting etc.
====== Thanks for your time,
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
==============================Original Headers============================== 8, 21 -- From hinmeigeng-at-hotmail.com Mon Jul 25 15:43:59 2005 8, 21 -- Received: from hotmail.com (bay101-f30.bay101.hotmail.com [64.4.56.40]) 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6PKhx61009344 8, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 25 Jul 2005 15:43:59 -0500 8, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 8, 21 -- Mon, 25 Jul 2005 13:43:58 -0700 8, 21 -- Message-ID: {BAY101-F30CFFD9E2F324B51359788CACA0-at-phx.gbl} 8, 21 -- Received: from 64.4.56.204 by by101fd.bay101.hotmail.msn.com with HTTP; 8, 21 -- Mon, 25 Jul 2005 20:43:57 GMT 8, 21 -- X-Originating-IP: [64.4.56.204] 8, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 8, 21 -- X-Sender: hinmeigeng-at-hotmail.com 8, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk 8, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 8, 21 -- To: Microscopy-at-MSA.Microscopy.Com 8, 21 -- Cc: R.H.Olley-at-reading.ac.uk 8, 21 -- Subject: ESEM of soil 8, 21 -- Date: Mon, 25 Jul 2005 20:43:57 +0000 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; format=flowed 8, 21 -- X-OriginalArrivalTime: 25 Jul 2005 20:43:58.0068 (UTC) FILETIME=[94929B40:01C59159] ==============================End of - Headers==============================
==============================Original Headers============================== 18, 33 -- From innap-at-savion.huji.ac.il Tue Jul 26 01:34:57 2005 18, 33 -- Received: from mail2.cc.huji.ac.il (real-outmail.cc.huji.ac.il [132.64.1.18]) 18, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6Q6YuU7019768 18, 33 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 01:34:57 -0500 18, 33 -- Received: from mail2.cc.huji.ac.il (localhost [127.0.0.1]) 18, 33 -- by mail2.cc.huji.ac.il (Postfix) with SMTP id 732621E1273; 18, 33 -- Tue, 26 Jul 2005 09:34:55 +0300 (IDT) 18, 33 -- Received: by mail2.cc.huji.ac.il (Postfix, from userid 31998) 18, 33 -- id 64E821E342A; Tue, 26 Jul 2005 09:34:55 +0300 (IDT) 18, 33 -- Received: from mango2.hustaff.huji.local (mango2.cc.huji.ac.il [132.64.16.16]) 18, 33 -- by mail2.cc.huji.ac.il (Postfix) with ESMTP id 6B8171E1273 18, 33 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:34:51 +0300 (IDT) 18, 33 -- Received: from MANGO1.hustaff.huji.local ([132.64.16.23]) by mango2.hustaff.huji.local with Microsoft SMTPSVC(6.0.3790.211); 18, 33 -- Tue, 26 Jul 2005 09:34:51 +0300 18, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 18, 33 -- Content-class: urn:content-classes:message 18, 33 -- MIME-Version: 1.0 18, 33 -- Content-Type: text/plain; 18, 33 -- charset="us-ascii" 18, 33 -- Subject: RE: [Microscopy] ESEM of soil 18, 33 -- Date: Tue, 26 Jul 2005 09:34:51 +0300 18, 33 -- Message-ID: {FCAF0955B591E644BEDEE450C0B58F410C36C1-at-MANGO1.hustaff.huji.local} 18, 33 -- X-MS-Has-Attach: 18, 33 -- X-MS-TNEF-Correlator: 18, 33 -- Thread-Topic: [Microscopy] ESEM of soil 18, 33 -- Thread-Index: AcWRWfN+M930BrzrRMu3HdJTDGGTjQAUhDxA 18, 33 -- From: "Inna Popov" {innap-at-savion.huji.ac.il} 18, 33 -- To: {microscopy-at-microscopy.com} 18, 33 -- X-OriginalArrivalTime: 26 Jul 2005 06:34:51.0396 (UTC) FILETIME=[20672440:01C591AC] 18, 33 -- X-Spam-Level: 18, 33 -- X-Virus: scanned by VAMS 2005 version 8.806 from Mon Jul 25 18:34:48 2005 CEST 18, 33 -- Content-Transfer-Encoding: 8bit 18, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6Q6YuU7019768 ==============================End of - Headers==============================
How about taking a ~0.25 latex and diluting it until you have an even monolayer (~30:1 distilled water). Then try coating you should NOT be able to visualise ANY structure!
You need to remove the chromium oxide prior to applying a coat. Shutter the target and sputter until the plasma reaches a very nice light blue colour (once seen never forgotten) then remove the shutter and coat for just a few seconds.
Good luck as my information is how we ran the prototype so there may be a better route today?
Regards
Steve
PS see you in July it seems - great
----- Original Message ----- X-from: {liz.girvan-at-stonebow.otago.ac.nz} To: {protrain-at-emcourses.com} Sent: Tuesday, July 26, 2005 5:42 AM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005 at 12:09:42 ---------------------------------------------------------------------------
Email: mccaulak-at-wfu.edu Name: Anita McCauley
Organization: Wake Forest University
Title-Subject: [Filtered] MListserver: user charges for SEM and related equipment
Question: I run a small microscopy facility in which equipment is normally made available to users free of charge. We are currently developing a relationship with an outside user and would like to generate a usage fee list so that we can recover costs for equipment wear and consumables.
Rather than pulling numbers out of thin air, I would like to know what others charge for the use of an SEM, a critical point dryer, and a sputter coater. Tech time does not need to be included. Hopefully with this information, I will be able to put together a reasonable usage fee list.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005 at 12:19:54 ---------------------------------------------------------------------------
Email: mccaulak-at-wfu.edu Name: Anita McCauley
Organization: Wake Forest University
Title-Subject: [Filtered] MListserver: video camera for stereomicroscope
Question: I am looking to add a video camera to an existing Olympus SZX12 stereomicroscope. I would like to consider cameras ranging from consumer-grade cameras bought at big-box electronic stores to those made for scientific applications. Currently, I am having trouble finding much information regarding scientific-grade video cameras.
I would appreciate hearing from folks that have been using either type of camera on a stereomicroscope, the pros and cons to their set-ups, and whether they are happy with their configuration.
I've been using two different, commercially available Sony digital videocameras with success on both Zeiss and Leica stereomicroscopes. The only disadvantage I experienced is that the cameras have difficulties when filming samples which have both dark and very light spots. However, with a little rearrangment of the light setup, things could be quite solved! It of course also depends on the purpose: analysis or imaging. But for imagingm the quality is more than enough to be accepted by top-rated journals! Best,
Sven Terclavers
Quoting mccaulak-at-wfu.edu:
} } } } ---------------------------------------------------------------------- ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- ------ } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (mccaulak-at-wfu.edu) from } http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005 at } 12:19:54 } ---------------------------------------------------------------------- ----- } } Email: mccaulak-at-wfu.edu } Name: Anita McCauley } } Organization: Wake Forest University } } Title-Subject: [Filtered] MListserver: video camera for } stereomicroscope } } Question: I am looking to add a video camera to an existing Olympus } SZX12 stereomicroscope. I would like to consider cameras ranging } from consumer-grade cameras bought at big-box electronic stores to } those made for scientific applications. Currently, I am having } trouble finding much information regarding scientific-grade video } cameras. } } I would appreciate hearing from folks that have been using either } type of camera on a stereomicroscope, the pros and cons to their } set-ups, and whether they are happy with their configuration. } } Thanks for the help. } } ---------------------------------------------------------------------- ----- } } ==============================Original } Headers============================== } 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:22 2005 } 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6QDoL8n008645 } 8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 08:50: 22 } -0500 } 8, 12 -- Mime-Version: 1.0 } 8, 12 -- X-Sender: (Unverified) } 8, 12 -- Message-Id: {p06110404bf0bf00e6734-at-[206.69.208.22]} } 8, 12 -- Date: Tue, 26 Jul 2005 08:50:21 -0500 } 8, 12 -- To: microscopy-at-microscopy.com } 8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver) } 8, 12 -- Subject: viaWWW: video camera for stereomicroscope } 8, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 10, 29 -- From Sven.Terclavers-at-med.kuleuven.be Tue Jul 26 09:14:07 2005 10, 29 -- Received: from thumbler.kulnet.kuleuven.ac.be (thumbler.kulnet.kuleuven.ac.be [134.58.240.45]) 10, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QEE6tS023423 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:14:07 -0500 10, 29 -- Received: from localhost (localhost [127.0.0.1]) 10, 29 -- by thumbler.kulnet.kuleuven.ac.be (Postfix) with ESMTP id 51C0F137E64 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:14:06 +0200 (CEST) 10, 29 -- Received: from antonius.kulnet.kuleuven.ac.be (antonius.kulnet.kuleuven.ac.be [134.58.240.73]) 10, 29 -- by thumbler.kulnet.kuleuven.ac.be (Postfix) with ESMTP id 60F5A137E74 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:14:05 +0200 (CEST) 10, 29 -- Received: from localhost (webmail2.cc.kuleuven.ac.be [134.58.242.4]) 10, 29 -- by antonius.kulnet.kuleuven.ac.be (Postfix) with ESMTP id 378344C2C5 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:14:05 +0200 (CEST) 10, 29 -- Received: from tom.med.kuleuven.ac.be (tom.med.kuleuven.ac.be [134.58.245.165]) 10, 29 -- by webmail2.kuleuven.be (IMP) with HTTP 10, 29 -- for {u0031291-at-127.0.0.1} ; Tue, 26 Jul 2005 16:13:51 +0200 10, 29 -- Message-ID: {1122387231.42e6451f43e81-at-webmail2.kuleuven.be} 10, 29 -- Date: Tue, 26 Jul 2005 16:13:51 +0200 10, 29 -- From: Sven Terclavers {Sven.Terclavers-at-med.kuleuven.be} 10, 29 -- To: microscopy-at-microscopy.com 10, 29 -- Subject: Re: [Microscopy] viaWWW: video camera for stereomicroscope 10, 29 -- References: {200507261353.j6QDrUIE020974-at-ns.microscopy.com} 10, 29 -- In-Reply-To: {200507261353.j6QDrUIE020974-at-ns.microscopy.com} 10, 29 -- MIME-Version: 1.0 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- User-Agent: Internet Messaging Program (IMP) 3.2-cvs 10, 29 -- X-Originating-IP: 134.58.245.165 10, 29 -- X-Virus-Scanned: by KULeuven Antivirus Cluster ==============================End of - Headers==============================
I have been in the (possibly foolish) process of restoring a 1991-era Leitz AMC AF microscope with the intention of building up an automated image capture/tiling system. Things have been going moderately well, and most of the system is finally ready to go. However, I've encountered a curious problem: the microscope controller is not particularly configurable, and will not release the autofocus unit for normal function until *after* it has gone completely through its stage homing procedure. And, since I have changed over to a stepper stage and a more modern PC-based controller, the old servo-driven stage is no longer even present in the system.
I have obtained all the documentation that is available from Leitz, and have been through it with a fine-toothed comb: no mention is ever made of using only the autofocus as a standalone unit. If anyone has any experience with hacking on the various mid-90s Leitz AF hardware in any of its many manifestations (the AMC AF, CDV, LIS, MPV-SP), I'd very much like to spend a few minutes picking your brain via email or telephone.
My plan B is to simply scrap the AF unit altogether and go with the digital video based solution offered by the new controller. However, if I can save myself a few hundred dollars (and justify having purchased the unit to start with!), I'd like to press the original AF hardware into use if possible. Many thanks in advance for anyone willing to spend a few minutes on this topic: please contact me offlist at skod-at-ises-llc.com.
Scott Griffith ISES-LLC 9745 Steeplechase Drive Franktown, CO 80116 303-660-2541 voice 303-660-2542 fax skod-at-ises-llc.com
==============================Original Headers============================== 5, 17 -- From skod-at-ises-llc.com Tue Jul 26 10:24:00 2005 5, 17 -- Received: from trenco2.ises-llc.com ([204.31.68.59]) 5, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QFNtW7000424 5, 17 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 10:23:55 -0500 5, 17 -- Received: from localhost (thrale.ises-llc.com [192.168.168.10]) 5, 17 -- by trenco2.ises-llc.com (8.12.10/8.12.10) with ESMTP id j6QF6f5F021342 5, 17 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:06:41 -0600 (MDT) 5, 17 -- To: microscopy-at-microscopy.com 5, 17 -- Subject: Would like to exchange email with experienced Leitz AF users 5, 17 -- Date: Tue, 26 Jul 2005 09:23:54 -0600 5, 17 -- From: "Scott Griffith" {skod-at-ises-llc.com} 5, 17 -- Organization: ISES-LLC 5, 17 -- Content-Type: text/plain; format=flowed; delsp=yes; charset=iso-8859-1 5, 17 -- MIME-Version: 1.0 5, 17 -- Content-Transfer-Encoding: 8bit 5, 17 -- Message-ID: {op.sui014jsgdwb11-at-localhost} 5, 17 -- User-Agent: Opera M2(BETA1)/8.02 (MacPPC, build 2129) ==============================End of - Headers==============================
You asked: } Question: ... snipppage ... We are currently developing a } relationship with an outside user and would like to generate } a usage fee list so that we can recover costs for equipment } wear and consumables. } } Rather than pulling numbers out of thin air, I would like to } know what others charge for the use of an SEM, a critical point } dryer, and a sputter coater. Tech time does not need to be } included. Hopefully with this information, I will be able to } put together a reasonable usage fee list.
Salaries and overhead ? If you don't recover _all_ costs, then your charges will be below actual cost to your funding agencies and therefore unfair to private microscopy labs that have to make a profit in order to survive in the marketplace.
You need to find out your true costs. Asking others to tell you the going rates amounts to a combination in restraint of trade between you and whoever responds or fails to object to this unfair trade practice. There are still antitrust laws in effect, I think.
Best regards, George Langford, Sc.D. Principal Consultant Amenex Associates, Inc. http://www.amenex.com/ amenex-at-amenex.com
==============================Original Headers============================== 7, 24 -- From amenex-at-amenex.com Tue Jul 26 10:38:01 2005 7, 24 -- Received: from mail08.voicenet.com (mail08.voicenet.com [207.103.0.34]) 7, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6QFc1iv008629 7, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 10:38:01 -0500 7, 24 -- Received: (qmail 7949 invoked from network); 26 Jul 2005 15:38:01 -0000 7, 24 -- Received: from email1.voicenet.com (207.103.0.55) 7, 24 -- by mail08.voicenet.com with SMTP; 26 Jul 2005 15:38:01 -0000 7, 24 -- Received: (from georgel-at-localhost) 7, 24 -- by email1.voicenet.com (8.11.6+Sun/8.11.6) id j6QFbwf01695; 7, 24 -- Tue, 26 Jul 2005 11:37:58 -0400 (EDT) 7, 24 -- Date: Tue, 26 Jul 2005 11:37:58 -0400 (EDT) 7, 24 -- Message-Id: {200507261537.j6QFbwf01695-at-email1.voicenet.com} 7, 24 -- X-Authentication-Warning: email1.voicenet.com: georgel set sender to amenex-at-amenex.com using -f 7, 24 -- From: "George Langford, Sc.D." {amenex-at-amenex.com} 7, 24 -- To: "Anita McCauley" {mccaulak-at-wfu.edu} 7, 24 -- CC: microscopy-at-microscopy.com, amenex-at-amenex.com 7, 24 -- Subject: Re: user charges for SEM and related equipment 7, 24 -- References: 7, 24 -- In-Reply-To: 7, 24 -- X-Mailer: Voicenet Webmail 7, 24 -- X-IPAddress: 67.100.47.123 7, 24 -- X-Sender: georgel-at-voicenet.com 7, 24 -- MIME-Version: 1.0 7, 24 -- Content-Type: text/plain; charset=iso-8859-1 ==============================End of - Headers==============================
Thanks for the feedback...I have been looking at some Sony MiniDVs and so its good to hear that folks have been able to use them successfully.
Anita K. McCauley, Ph.D. Director of Microscopy/Adj. Asst. Prof. PO Box 7325 Biology Department Wake Forest University Winston-Salem, NC 27109
-----Original Message----- X-from: Sven.Terclavers-at-med.kuleuven.be [mailto:Sven.Terclavers-at-med.kuleuven.be] Sent: Tuesday, July 26, 2005 10:17 AM To: mccaulak-at-wfu.edu
Dear Anita,
I've been using two different, commercially available Sony digital videocameras with success on both Zeiss and Leica stereomicroscopes. The only disadvantage I experienced is that the cameras have difficulties when filming samples which have both dark and very light spots. However, with a little rearrangment of the light setup, things could be quite solved! It of course also depends on the purpose: analysis or imaging. But for imagingm the quality is more than enough to be accepted by top-rated journals! Best,
Sven Terclavers
Quoting mccaulak-at-wfu.edu:
} } } } ---------------------------------------------------------------------- ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- ------ } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (mccaulak-at-wfu.edu) from } http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005 at } 12:19:54 } ---------------------------------------------------------------------- ----- } } Email: mccaulak-at-wfu.edu } Name: Anita McCauley } } Organization: Wake Forest University } } Title-Subject: [Filtered] MListserver: video camera for } stereomicroscope } } Question: I am looking to add a video camera to an existing Olympus } SZX12 stereomicroscope. I would like to consider cameras ranging } from consumer-grade cameras bought at big-box electronic stores to } those made for scientific applications. Currently, I am having } trouble finding much information regarding scientific-grade video } cameras. } } I would appreciate hearing from folks that have been using either } type of camera on a stereomicroscope, the pros and cons to their } set-ups, and whether they are happy with their configuration. } } Thanks for the help. } } ---------------------------------------------------------------------- ----- } } ==============================Original } Headers============================== } 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:22 2005 } 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6QDoL8n008645 } 8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 08:50: 22 } -0500 } 8, 12 -- Mime-Version: 1.0 } 8, 12 -- X-Sender: (Unverified) } 8, 12 -- Message-Id: {p06110404bf0bf00e6734-at-[206.69.208.22]} } 8, 12 -- Date: Tue, 26 Jul 2005 08:50:21 -0500 } 8, 12 -- To: microscopy-at-microscopy.com } 8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver) } 8, 12 -- Subject: viaWWW: video camera for stereomicroscope } 8, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 10, 29 -- From Sven.Terclavers-at-med.kuleuven.be Tue Jul 26 09:14:07 2005 10, 29 -- Received: from thumbler.kulnet.kuleuven.ac.be (thumbler.kulnet.kuleuven.ac.be [134.58.240.45]) 10, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QEE6tS023423 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:14:07 -0500 10, 29 -- Received: from localhost (localhost [127.0.0.1]) 10, 29 -- by thumbler.kulnet.kuleuven.ac.be (Postfix) with ESMTP id 51C0F137E64 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:14:06 +0200 (CEST) 10, 29 -- Received: from antonius.kulnet.kuleuven.ac.be (antonius.kulnet.kuleuven.ac.be [134.58.240.73]) 10, 29 -- by thumbler.kulnet.kuleuven.ac.be (Postfix) with ESMTP id 60F5A137E74 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:14:05 +0200 (CEST) 10, 29 -- Received: from localhost (webmail2.cc.kuleuven.ac.be [134.58.242.4]) 10, 29 -- by antonius.kulnet.kuleuven.ac.be (Postfix) with ESMTP id 378344C2C5 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:14:05 +0200 (CEST) 10, 29 -- Received: from tom.med.kuleuven.ac.be (tom.med.kuleuven.ac.be [134.58.245.165]) 10, 29 -- by webmail2.kuleuven.be (IMP) with HTTP 10, 29 -- for {u0031291-at-127.0.0.1} ; Tue, 26 Jul 2005 16:13:51 +0200 10, 29 -- Message-ID: {1122387231.42e6451f43e81-at-webmail2.kuleuven.be} 10, 29 -- Date: Tue, 26 Jul 2005 16:13:51 +0200 10, 29 -- From: Sven Terclavers {Sven.Terclavers-at-med.kuleuven.be} 10, 29 -- To: microscopy-at-microscopy.com 10, 29 -- Subject: Re: [Microscopy] viaWWW: video camera for stereomicroscope 10, 29 -- References: {200507261353.j6QDrUIE020974-at-ns.microscopy.com} 10, 29 -- In-Reply-To: {200507261353.j6QDrUIE020974-at-ns.microscopy.com} 10, 29 -- MIME-Version: 1.0 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- User-Agent: Internet Messaging Program (IMP) 3.2-cvs 10, 29 -- X-Originating-IP: 134.58.245.165 10, 29 -- X-Virus-Scanned: by KULeuven Antivirus Cluster ==============================End of - Headers==============================
==============================Original Headers============================== 19, 21 -- From mccaulak-at-wfu.edu Tue Jul 26 10:56:57 2005 19, 21 -- Received: from f4n35.wfunet.wfu.edu (f4n35.wfunet.wfu.edu [152.17.48.111]) 19, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QFuv1u016580 19, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 10:56:57 -0500 19, 21 -- Received: from mccaulak2004 (mccaulak-2004.dhcp.wfu.edu [10.122.1.11]) 19, 21 -- by f4n35.wfunet.wfu.edu (8.12.11/8.12.11) with ESMTP id j6QFuu1T002275 19, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:56:56 -0400 19, 21 -- Message-Id: {200507261556.j6QFuu1T002275-at-f4n35.wfunet.wfu.edu} 19, 21 -- Reply-To: {mccaulak-at-wfu.edu} 19, 21 -- From: "Anita K. McCauley" {mccaulak-at-wfu.edu} 19, 21 -- To: {microscopy-at-microscopy.com} 19, 21 -- Subject: RE: [Microscopy] Re: viaWWW: video camera for stereomicroscope 19, 21 -- Date: Tue, 26 Jul 2005 11:56:56 -0400 19, 21 -- MIME-Version: 1.0 19, 21 -- Content-Type: text/plain; 19, 21 -- charset="us-ascii" 19, 21 -- Content-Transfer-Encoding: 7bit 19, 21 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 19, 21 -- Thread-Index: AcWR7Kn6aNC+R2hKSUKHrv1m792r2QADdaXw 19, 21 -- In-Reply-To: {200507261416.j6QEGhp9027252-at-ns.microscopy.com} 19, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 ==============================End of - Headers==============================
I'm trying to express in grams the amount of NaOH that I'd need for 100 ml of saturated NaOH in ethanol.
Does anyone know this off the top of their head without me having to find a reference or figure it out with the real pellets.
This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
==============================Original Headers============================== 4, 19 -- From GBurgess-at-exchange.hsc.mb.ca Tue Jul 26 11:01:10 2005 4, 19 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 4, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QG1AJ2024276 4, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:01:10 -0500 4, 19 -- Received: from mudslide.hsc.mb.ca (unverified [172.16.6.136]) by 4, 19 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 4, 19 -- {B0013633890-at-hscxntmx0003.hsc.mb.ca} for {microscopy-at-microscopy.com} ;Tue, 4, 19 -- 26 Jul 2005 11:03:48 -0500 4, 19 -- Received: by mudslide.hsc.mb.ca with Internet Mail Service (5.5.2653.19)id 4, 19 -- {3F02CF45} ; Tue, 26 Jul 2005 11:01:09 -0500 4, 19 -- Message-ID: {00A937989100304A83A058F6C45873FF32A24E-at-hscxntmx0005} 4, 19 -- Date: Tue, 26 Jul 2005 10:59:25 -0500 4, 19 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 4, 19 -- Subject: saturated NaOH 4, 19 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 4, 19 -- X-Mailer: Internet Mail Service (5.5.2653.19) 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: text/plain; 4, 19 -- charset="iso-8859-1" ==============================End of - Headers==============================
You do have to be careful about this topic on the list. There is a concern (rightly so) from for-profit companies that Universities will deprive them of business by undercutting fees. The question you ask is fairly complex. You do need to look at all costs associated with your facility (service contracts, consumables, telephone, etc) and come up with a rate structure that would cover these costs if you needed to charge all current users.
Then look at your internal users as subsidized. To the base costs, add the percentage used by your university for indirect costs. This would be the same amount charged to grants to cover building costs, lights, heating, etc. This can be as high as 50% in many institutions.
If you are doing the work than figure your salary, fringe benefits, etc. to get an hourly rate that compensates totally for your time. Tack on a few extra dollars when figuring consumable costs as prices go up regularly and you need to recoup these costs without having to change your whole rate structure.
For example: Assume an SEM with $15,000 service contract and consumables (filaments, nitrogen, etc) of $2000/yr. Figure 1000 hours of billable use time results in an hourly charge of $17/hr. Add time you spend maintaining the instrument (aligning, changing filaments, other non-billable time adding up to 1000hrs/yr) and this figure can easily double. Add the 50% overhead and you arrive at $51/hr.
This amount is very low compared to what for-profit labs must charge as they have to use capital to purchase the instrument in the first place and then depreciate it so they will have funds to replace when necessary. They also need to pay all costs associated with the physical structures, advertising, etc. This is why they are concerned about Universities undercutting their necessary charges. If you have no private labs near you than you can probably get away with the lower costs. Industry would be delighted with this amount/hr as their costs if they owned the machine would be much greater due to the depreciation, etc.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
On 7/26/05 8:52 AM, "mccaulak-at-wfu.edu" {mccaulak-at-wfu.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Below is the result of your feedback form (NJZFM-ultra-55). It was submitted } by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on } Monday, July 25, 2005 at 12:09:42 } --------------------------------------------------------------------------- } } Email: mccaulak-at-wfu.edu } Name: Anita McCauley } } Organization: Wake Forest University } } Title-Subject: [Filtered] MListserver: user charges for SEM and related } equipment } } Question: I run a small microscopy facility in which equipment is normally } made available to users free of charge. We are currently developing a } relationship with an outside user and would like to generate a usage fee list } so that we can recover costs for equipment wear and consumables. } } Rather than pulling numbers out of thin air, I would like to know what others } charge for the use of an SEM, a critical point dryer, and a sputter coater. } Tech time does not need to be included. Hopefully with this information, I } will be able to put together a reasonable usage fee list. } } Thanks. } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:01 2005 } 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QDo0XS007866 } 8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 08:50:00 -0500 } 8, 12 -- Mime-Version: 1.0 } 8, 12 -- X-Sender: (Unverified) } 8, 12 -- Message-Id: {p06110403bf0beff76201-at-[206.69.208.22]} } 8, 12 -- Date: Tue, 26 Jul 2005 08:49:59 -0500 } 8, 12 -- To: microscopy-at-microscopy.com } 8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver) } 8, 12 -- Subject: viaWWW: user charges for SEM and related equipment } 8, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 13, 21 -- From dsherman-at-purdue.edu Tue Jul 26 11:07:59 2005 13, 21 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 13, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QG7wJC032030 13, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:07:59 -0500 13, 21 -- Received: from [128.210.121.247] (lsmf07.btny.purdue.edu [128.210.121.247]) 13, 21 -- by mailhub131.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j6QG7vBU012473 13, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:07:58 -0500 13, 21 -- User-Agent: Microsoft-Entourage/11.1.0.040913 13, 21 -- Date: Tue, 26 Jul 2005 11:07:56 -0500 13, 21 -- Subject: Re: [Microscopy] viaWWW: user charges for SEM and related 13, 21 -- equipment 13, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 13, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 13, 21 -- Message-ID: {BF0BCA0C.7850%dsherman-at-purdue.edu} 13, 21 -- In-Reply-To: {200507261352.j6QDqQlK016597-at-ns.microscopy.com} 13, 21 -- Mime-version: 1.0 13, 21 -- Content-type: text/plain; 13, 21 -- charset="US-ASCII" 13, 21 -- Content-transfer-encoding: 7bit 13, 21 -- X-PMX-Version: 4.7.1.128075 13, 21 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
as i recall 52 gms of NaOH in 100ml DH20 will yield a saturated soln john
--- GBurgess-at-exchange.hsc.mb.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } I'm trying to express in grams the amount of NaOH } that I'd need for 100 ml } of saturated NaOH in ethanol. } } Does anyone know this off the top of their head } without me having to find a } reference or figure it out with the real pellets. } } This e-mail and/or any documents in this } transmission is intended for the address(s) only and } may contain legally privileged or confidential } information. Any unauthorized use, disclosure, } distribution, copying or dissemination is strictly } prohibited. If you receive this transmission in } error, please notify the sender immediately and } return the original. } } ==============================Original } Headers============================== } 4, 19 -- From GBurgess-at-exchange.hsc.mb.ca Tue Jul 26 } 11:01:10 2005 } 4, 19 -- Received: from hscxntmx0003.hsc.mb.ca } (hscxntmx0003.hsc.mb.ca [142.233.100.122]) } 4, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j6QG1AJ2024276 } 4, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 } Jul 2005 11:01:10 -0500 } 4, 19 -- Received: from mudslide.hsc.mb.ca } (unverified [172.16.6.136]) by } 4, 19 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS } 4.0.346.0) with ESMTP id } 4, 19 -- {B0013633890-at-hscxntmx0003.hsc.mb.ca} for } {microscopy-at-microscopy.com} ;Tue, } 4, 19 -- 26 Jul 2005 11:03:48 -0500 } 4, 19 -- Received: by mudslide.hsc.mb.ca with } Internet Mail Service (5.5.2653.19)id } 4, 19 -- {3F02CF45} ; Tue, 26 Jul 2005 11:01:09 } -0500 } 4, 19 -- Message-ID: } {00A937989100304A83A058F6C45873FF32A24E-at-hscxntmx0005} } 4, 19 -- Date: Tue, 26 Jul 2005 10:59:25 -0500 } 4, 19 -- From: Garry Burgess } {GBurgess-at-exchange.hsc.mb.ca} } 4, 19 -- Subject: saturated NaOH } 4, 19 -- To: "'microscopy-at-microscopy.com'" } {microscopy-at-microscopy.com} } 4, 19 -- X-Mailer: Internet Mail Service } (5.5.2653.19) } 4, 19 -- MIME-Version: 1.0 } 4, 19 -- Content-Type: text/plain; } 4, 19 -- charset="iso-8859-1" } ==============================End of - } Headers============================== }
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Also, contact Bill Miller at microbill-at-mohawk.net or by phone at (860)672-0068.
Just a reminder: remember that what makes a stereo microscope stereo is your looking with two eyes and that a camera will only "look" with one. You may need to adjust the lighting to make your objects look a little more 3D.
Good hunting!
Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
P. S. Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 10:59 AM 7/26/2005, mccaulak-at-wfu.edu wrote:
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==============================Original Headers============================== 12, 18 -- From bfoster-at-mme1.com Tue Jul 26 11:35:33 2005 12, 18 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95] (may be forged)) 12, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QGZXTO015393 12, 18 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:35:33 -0500 12, 18 -- Received: (qmail 5867 invoked from network); 26 Jul 2005 16:35:42 -0000 12, 18 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 12, 18 -- by enterprise.5starpro.com with SMTP; 26 Jul 2005 16:35:42 -0000 12, 18 -- Message-Id: {6.1.2.0.0.20050726112822.028374a8-at-mail.mme1.com} 12, 18 -- X-Sender: bfoster-at-mail.mme1.com 12, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 12, 18 -- Date: Tue, 26 Jul 2005 11:35:21 -0500 12, 18 -- To: microscopy-at-microscopy.com 12, 18 -- From: Barbara Foster {bfoster-at-mme1.com} 12, 18 -- Subject: Re: [Microscopy] viaWWW: video camera for stereomicroscope 12, 18 -- In-Reply-To: {200507261559.j6QFxcpd021180-at-ns.microscopy.com} 12, 18 -- References: {200507261559.j6QFxcpd021180-at-ns.microscopy.com} 12, 18 -- Mime-Version: 1.0 12, 18 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
"Consumer-grade" cameras these have built in non-removable lenses. Unless you are really straped for money you want a camera which makes use of the steroscope's optics (for which you spent alot more than the $0.5-$10 piece of plastic sitting on a consumer grade camera). In which case you are now looking for a C-mount camera.
Since you have the SZX-12 I would defintiely recommend getting a U-TV0.5XC-2; CCD CAMERA ADAPTER, for mounting a c-mount "video camera". It is very compact, and has built in parfocalizing optics (for matching the eye pieces to the camera view) which you set and forget. (You will have to determine if you need to add other compoonents to get a photo port on your SZX12. If you must go through the eye-piece for cost reasons there are some very nice video couplers for this. E.g. Thales Optem optemintl.com or Diagnostic Instruments www.diaginc.com )
Next up for "video camera" becomes what do you want to do with it? What do you mean by "video camera"? Do you truely mean "video" as in television, VCR's, "live" motion video?
Standard NTSC (north american television) show you can display it on a "TV monitor", or do you want to use a computer to capture images? A tv monitor will require an analog input (or DV input), and you will need a capture card (Grabber) or a digital camera output (DV or IEEE-1394/firewire, etc.) to convert this to something a computer can use.
Still images or motion analysis? Still images moves into "digtal cameras" but motion anaylsis starts dealing with "frame" rates (for normal TV-monitors you need Standard NTSC = 30 frames/sec and PAL 25 frames/sec - yes, interlaced).
Monochrome or color?
Then you get into resolution standard NTSC is 640x480pixels good and inexpensive for TV monitors but not very good for "publication". Higher resolution 1024 x 768 or 1280 x 980 or HDTV
Low light (fluorescence) vs normal bright-field / reflected light work?
Hmm, looking around I found www.theimagingsource.com and it has lots of c-mount camera choices and pricing! (I have never shoped there yet but it does look very interesting) There are lots of other sources for "video" camera out there. and pricing will range from $300 to $5,000 on up into pricing over $200,000. I am sure you will here from a few vendors shortly.
In video cameras we here have a Dage MTI camera, two Sony cameras, and a Javalin camera collected through the years (none of which are available any longer)
Good luck and feel free to contact me back with more questions.
Note: We're a univeristy EM Faciltiy I do not sell any video equipment nor hold any financial interests in any company mentioned above . . . at least I don't think I do.
On 26 Jul 2005, at 8:51, mccaulak-at-wfu.edu wrote:
} Email: mccaulak-at-wfu.edu } Name: Anita McCauley } } Organization: Wake Forest University } } Title-Subject: [Filtered] MListserver: video camera for stereomicroscope } } Question: I am looking to add a video camera to an existing } Olympus SZX12 stereomicroscope. I would like to consider cameras } ranging from consumer-grade cameras bought at big-box electronic } stores to those made for scientific applications. Currently, I am } having trouble finding much information regarding scientific-grade } video cameras. } } I would appreciate hearing from folks that have been using either } type of camera on a stereomicroscope, the pros and cons to their } set-ups, and whether they are happy with their configuration. } } Thanks for the help. } } ---------------------------------------------------------------------------
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 350 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 18, 25 -- From edelmare-at-muohio.edu Tue Jul 26 11:45:32 2005 18, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 18, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QGjWht023157 18, 25 -- for {microscopy-at-Microscopy.com} ; Tue, 26 Jul 2005 11:45:32 -0500 18, 25 -- Received: from muw2k05 (muw2k05.mcs.muohio.edu [134.53.6.19]) 18, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with SMTP id j6QGjVoT007369 18, 25 -- for {microscopy-at-Microscopy.com} ; Tue, 26 Jul 2005 12:45:31 -0400 18, 25 -- Received: From mcsaix02.mcs.muohio.edu ([134.53.253.26]) by muw2k05 (WebShield SMTP v4.5 MR1a P0803.345); 18, 25 -- id 1122395855265; Tue, 26 Jul 2005 12:37:35 -0400 18, 25 -- Received: from emf03 ([134.53.14.119]) 18, 25 -- by mcsaix02.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6QGhatu212286; 18, 25 -- Tue, 26 Jul 2005 12:43:36 -0400 18, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 18, 25 -- To: mccaulak-at-wfu.edu 18, 25 -- Date: Tue, 26 Jul 2005 12:45:27 -0400 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-type: text/plain; charset=US-ASCII 18, 25 -- Content-transfer-encoding: 7BIT 18, 25 -- Subject: Re: [Microscopy] viaWWW: video camera for stereomicroscope 18, 25 -- CC: microscopy-at-Microscopy.com 18, 25 -- Message-ID: {42E63067.31791.4917BF-at-localhost} 18, 25 -- Priority: normal 18, 25 -- In-reply-to: {200507261351.j6QDp2mA011351-at-ns.microscopy.com} 18, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 18, 25 -- X-Scanned-By: MIMEDefang 2.45 ==============================End of - Headers==============================
Debby Sherman wrote "If you have no private labs near you than you can probably get away with the lower [prices]." I've always been concerned about the fuzzy definition of "near-ness". My AFM analytical services business in Indianapolis, Indiana has a worldwide clientele because express courier services extend our reach. This is fortunate, for if I had to depend on the business generated within the state of Indiana I would have gone out of business many years ago.
George Langford comments that a survey of going rates might violate antitrust laws. Nevertheless, I think the policy of government granting agencies requires this. Remember: no one ever said the government has to be self-consistent.
The unfortunate aspect of university services is that they really are driving the commercial labs out of business. I can say with confidence that the number of commercial, for-profit labs providing AFM service in the US has declined significantly in the last few years. I know this because I have been buying their AFM equipment for resale.
regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
----- Original Message ----- From: dsherman-at-purdue.edu To: donc-at-asmicro.com Sent: Tuesday, July 26, 2005 11:11 AM Subject: [a] [Microscopy] Re: viaWWW: user charges for SEM and related
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Anita,
You do have to be careful about this topic on the list. There is a concern (rightly so) from for-profit companies that Universities will deprive them of business by undercutting fees. The question you ask is fairly complex. You do need to look at all costs associated with your facility (service contracts, consumables, telephone, etc) and come up with a rate structure that would cover these costs if you needed to charge all current users.
Then look at your internal users as subsidized. To the base costs, add the percentage used by your university for indirect costs. This would be the same amount charged to grants to cover building costs, lights, heating, etc. This can be as high as 50% in many institutions.
If you are doing the work than figure your salary, fringe benefits, etc. to get an hourly rate that compensates totally for your time. Tack on a few extra dollars when figuring consumable costs as prices go up regularly and you need to recoup these costs without having to change your whole rate structure.
For example: Assume an SEM with $15,000 service contract and consumables (filaments, nitrogen, etc) of $2000/yr. Figure 1000 hours of billable use time results in an hourly charge of $17/hr. Add time you spend maintaining the instrument (aligning, changing filaments, other non-billable time adding up to 1000hrs/yr) and this figure can easily double. Add the 50% overhead and you arrive at $51/hr.
This amount is very low compared to what for-profit labs must charge as they have to use capital to purchase the instrument in the first place and then depreciate it so they will have funds to replace when necessary. They also need to pay all costs associated with the physical structures, advertising, etc. This is why they are concerned about Universities undercutting their necessary charges. If you have no private labs near you than you can probably get away with the lower costs. Industry would be delighted with this amount/hr as their costs if they owned the machine would be much greater due to the depreciation, etc.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
On 7/26/05 8:52 AM, "mccaulak-at-wfu.edu" {mccaulak-at-wfu.edu} wrote:
} } }
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} -------------------------------------------------------------------------- -- } } Below is the result of your feedback form (NJZFM-ultra-55). It was submitted } by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on } Monday, July 25, 2005 at 12:09:42
} -------------------------------------------------------------------------- - } } Email: mccaulak-at-wfu.edu } Name: Anita McCauley } } Organization: Wake Forest University } } Title-Subject: [Filtered] MListserver: user charges for SEM and related } equipment } } Question: I run a small microscopy facility in which equipment is normally } made available to users free of charge. We are currently developing a } relationship with an outside user and would like to generate a usage fee list } so that we can recover costs for equipment wear and consumables. } } Rather than pulling numbers out of thin air, I would like to know what others } charge for the use of an SEM, a critical point dryer, and a sputter coater. } Tech time does not need to be included. Hopefully with this information, I } will be able to put together a reasonable usage fee list. } } Thanks. }
} -------------------------------------------------------------------------- - } } ==============================Original Headers============================== } 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:01 2005 } 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QDo0XS007866 } 8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 08:50:00 -0500 } 8, 12 -- Mime-Version: 1.0 } 8, 12 -- X-Sender: (Unverified) } 8, 12 -- Message-Id: {p06110403bf0beff76201-at-[206.69.208.22]} } 8, 12 -- Date: Tue, 26 Jul 2005 08:49:59 -0500 } 8, 12 -- To: microscopy-at-microscopy.com } 8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver) } 8, 12 -- Subject: viaWWW: user charges for SEM and related equipment } 8, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 13, 21 -- From dsherman-at-purdue.edu Tue Jul 26 11:07:59 2005 13, 21 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 13, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QG7wJC032030 13, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:07:59 -0500 13, 21 -- Received: from [128.210.121.247] (lsmf07.btny.purdue.edu [128.210.121.247]) 13, 21 -- by mailhub131.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j6QG7vBU012473 13, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:07:58 -0500 13, 21 -- User-Agent: Microsoft-Entourage/11.1.0.040913 13, 21 -- Date: Tue, 26 Jul 2005 11:07:56 -0500 13, 21 -- Subject: Re: [Microscopy] viaWWW: user charges for SEM and related 13, 21 -- equipment 13, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 13, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 13, 21 -- Message-ID: {BF0BCA0C.7850%dsherman-at-purdue.edu} 13, 21 -- In-Reply-To: {200507261352.j6QDqQlK016597-at-ns.microscopy.com} 13, 21 -- Mime-version: 1.0 13, 21 -- Content-type: text/plain; 13, 21 -- charset="US-ASCII" 13, 21 -- Content-transfer-encoding: 7bit 13, 21 -- X-PMX-Version: 4.7.1.128075 13, 21 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
==============================Original Headers============================== 35, 21 -- From donc-at-asmicro.com Tue Jul 26 11:49:55 2005 35, 21 -- Received: from smtp104.sbc.mail.re2.yahoo.com (smtp104.sbc.mail.re2.yahoo.com [68.142.229.101]) 35, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6QGnsVr029289 35, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:49:54 -0500 35, 21 -- Received: (qmail 9605 invoked from network); 26 Jul 2005 16:49:53 -0000 35, 21 -- Received: from unknown (HELO ASM11) (asmicro-at-sbcglobal.net-at-68.254.139.10 with login) 35, 21 -- by smtp104.sbc.mail.re2.yahoo.com with SMTP; 26 Jul 2005 16:49:52 -0000 35, 21 -- Message-ID: {009801c59201$8bbf9ba0$c901a8c0-at-ASM11} 35, 21 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 35, 21 -- To: "Microscopy List" {microscopy-at-microscopy.com} 35, 21 -- References: {200507261611.j6QGBI4o006065-at-ns.microscopy.com} 35, 21 -- Subject: Re: [Microscopy] user charges for SEM and related 35, 21 -- Date: Tue, 26 Jul 2005 11:46:04 -0500 35, 21 -- MIME-Version: 1.0 35, 21 -- Content-Type: text/plain; 35, 21 -- charset="iso-8859-1" 35, 21 -- Content-Transfer-Encoding: 7bit 35, 21 -- X-Priority: 3 35, 21 -- X-MSMail-Priority: Normal 35, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1506 35, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 ==============================End of - Headers==============================
I apologize to the list for posting Marilyn vos Savant's statement on microscopy. I just wanted to share the article because I thought it was humorous...I shall refrain from such postings. best, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
George and Debby are correct. It is not fair to independent (=non taxpayer subsidized) labs when a university facility charges artificially low fees for instruments bought with public money, and it is actually a violation of federal law for them to compete in this manner. I remember that in the '70s Okla. State University's 'dairy barn' had to stop selling dairy products at prices below those of local grocery stores. However more recently I couldn't get business for my own SEM services because our Lafayette, IN industries tell me they can get their work done at our local university for little or no cost. But might as well mention the illegality of this practice to a brick wall. Grrrrrrrr.
Paul Grover Erstwhile Chief Microscopist & Bottle Washer Microvista Laboratory
------------------------------------------------------------------------ No matter how much cats fight, there always seem to be plenty of kittens. - A. Lincoln
==============================Original Headers============================== 6, 24 -- From pgrover-at-bilbo.bio.purdue.edu Tue Jul 26 11:54:45 2005 6, 24 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 6, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QGsiKs010447 6, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:54:45 -0500 6, 24 -- Received: from paklabpgrover (dhcp155-220.bio.purdue.edu [128.210.155.220]) 6, 24 -- by mailhub131.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j6QGsicN011702 6, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 11:54:44 -0500 6, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 6, 24 -- To: {microscopy-at-microscopy.com} 6, 24 -- Subject: [Microscopy] Re: user charges for SEM and related equipment 6, 24 -- Date: Tue, 26 Jul 2005 11:54:48 -0500 6, 24 -- Message-ID: {000101c59202$bb74a1a0$dc9bd280-at-paklabpgrover} 6, 24 -- MIME-Version: 1.0 6, 24 -- Content-Type: text/plain; 6, 24 -- charset="us-ascii" 6, 24 -- X-Priority: 3 (Normal) 6, 24 -- X-MSMail-Priority: Normal 6, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 6, 24 -- Importance: Normal 6, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 6, 24 -- X-PMX-Version: 4.7.1.128075 6, 24 -- X-PerlMx-Virus-Scanned: Yes 6, 24 -- Content-Transfer-Encoding: 8bit 6, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6QGsiKs010447 ==============================End of - Headers==============================
We must remember, that Microscopists do not live by electrons alone...a little levity is ALWAYS WELCOME! Especially at the National Labs!
Dorrance McLean Sandia National Laboratories Livermore, CA
-----Original Message----- X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu] Sent: Tuesday, July 26, 2005 9:52 AM To: McLean, Dorrance
I apologize to the list for posting Marilyn vos Savant's statement on microscopy. I just wanted to share the article because I thought it was humorous...I shall refrain from such postings. best, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
If you have any announcements to be included in the daily newsleter of the meeting, please email me before noon (EST) on Friday or drop your copy by the LAC Headquarters at the convention center.
Thanks, Greg
-- Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 Phone: 352-392-1295 Fax: 352 846 0251
==============================Original Headers============================== 5, 21 -- From gwe-at-ufl.edu Tue Jul 26 12:08:11 2005 5, 21 -- Received: from smtp.ufl.edu (sp11en1.nerdc.ufl.edu [128.227.74.11]) 5, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QH8Amp025135 5, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 26 Jul 2005 12:08:11 -0500 5, 21 -- Received: from [127.0.0.1] (pc2524c.dhcp.clas.ufl.edu [128.227.60.197]) 5, 21 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j6QH86r6117514 5, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 26 Jul 2005 13:08:08 -0400 5, 21 -- Message-ID: {42E66DED.5010805-at-ufl.edu} 5, 21 -- Date: Tue, 26 Jul 2005 13:07:57 -0400 5, 21 -- From: Greg Erdos {gwe-at-ufl.edu} 5, 21 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 5, 21 -- X-Accept-Language: en-us, en 5, 21 -- MIME-Version: 1.0 5, 21 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 5, 21 -- Subject: M&M 2005 Attendees 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- X-Spam-Status: hits=-1.428, required=5, tests=BAYES_20 5, 21 -- X-UFL-Spam-Status: hits=-1.428, required=5, tests=BAYES_20 5, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 5, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Don't apologize - it was a humorous (and brief) posting. (I noticed the complainers were mostly men- significant??). Keep 'em coming!
Alan Grant Dept. of Geological Sciences and Geological Engineering Queen's University Kingston ON Canada K7L 3N6
beth-at-plantbio.uga.edu wrote:
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==============================Original Headers============================== 5, 19 -- From granta-at-geol.queensu.ca Tue Jul 26 12:19:39 2005 5, 19 -- Received: from post.queensu.ca (post.QueensU.CA [130.15.126.6]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QHJdQg012384 5, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 12:19:39 -0500 5, 19 -- Received: from [130.15.110.53] (grantspc.geol.QueensU.CA [130.15.110.53]) 5, 19 -- by post.queensu.ca (8.13.1/8.13.1) with ESMTP id j6QHJcmE001711 5, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 13:19:38 -0400 (EDT) 5, 19 -- Message-ID: {42E670B4.7050306-at-geol.queensu.ca} 5, 19 -- Date: Tue, 26 Jul 2005 13:19:48 -0400 5, 19 -- From: Alan Grant {granta-at-geol.queensu.ca} 5, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.1) Gecko/20040707 5, 19 -- X-Accept-Language: en-us, en 5, 19 -- MIME-Version: 1.0 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- Subject: Re: [Microscopy] sorry about that 5, 19 -- References: {200507261655.j6QGtdeM014287-at-ns.microscopy.com} 5, 19 -- In-Reply-To: {200507261655.j6QGtdeM014287-at-ns.microscopy.com} 5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Beth: I noticed that a critical comment about this (took too much of his time?) took the time to post two times on the topic. I think some people take themselves too seriously.
Maureen Petersen Duke Univ.
} From: beth-at-plantbio.uga.edu } Reply-To: microscopy-at-microscopy.com } To: Maureen_Petersen-at-msn.com } Subject: [Microscopy] sorry about that } Date: Tue, 26 Jul 2005 11:52:00 -0500 } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 5, 20 -- From maureen_petersen-at-msn.com Tue Jul 26 15:35:38 2005 5, 20 -- Received: from hotmail.com (bay107-f16.bay107.hotmail.com [64.4.51.26]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QKZcHw031186 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 15:35:38 -0500 5, 20 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 5, 20 -- Tue, 26 Jul 2005 13:35:37 -0700 5, 20 -- Message-ID: {BAY107-F16DB732A64FA2E7B06223DF1CD0-at-phx.gbl} 5, 20 -- Received: from 64.4.51.220 by by107fd.bay107.hotmail.msn.com with HTTP; 5, 20 -- Tue, 26 Jul 2005 20:35:37 GMT 5, 20 -- X-Originating-IP: [64.4.51.220] 5, 20 -- X-Originating-Email: [maureen_petersen-at-msn.com] 5, 20 -- X-Sender: maureen_petersen-at-msn.com 5, 20 -- In-Reply-To: {200507261652.j6QGq0L7001954-at-ns.microscopy.com} 5, 20 -- From: "MAUREEN PETERSON" {maureen_petersen-at-msn.com} 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Subject: RE: [Microscopy] sorry about that 5, 20 -- Date: Tue, 26 Jul 2005 16:35:37 -0400 5, 20 -- Mime-Version: 1.0 5, 20 -- Content-Type: text/plain; format=flowed 5, 20 -- X-OriginalArrivalTime: 26 Jul 2005 20:35:37.0354 (UTC) FILETIME=[948976A0:01C59221] ==============================End of - Headers==============================
Don't be sorry: it was good posting and we need something humorous here from time to time. Sergey
At 09:53 AM 7/26/2005, you wrote:
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==============================Original Headers============================== 6, 25 -- From sryazant-at-ucla.edu Tue Jul 26 15:35:54 2005 6, 25 -- Received: from smtp-6.smtp.ucla.edu (smtp-6.smtp.ucla.edu [169.232.48.138]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QKZrHX031551 6, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 15:35:54 -0500 6, 25 -- Received: from mail.ucla.edu (mail.ucla.edu [169.232.48.141]) 6, 25 -- by smtp-6.smtp.ucla.edu (8.13.4/8.13.4) with ESMTP id j6QKZqkE030381 6, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 13:35:52 -0700 6, 25 -- Received: from kopoba.ucla.edu (ts14-189.dialup.bol.ucla.edu [169.232.226.73]) 6, 25 -- (authenticated bits=0) 6, 25 -- by mail.ucla.edu (8.13.4/8.13.4) with ESMTP id j6QKZiH5007892 6, 25 -- (version=TLSv1/SSLv3 cipher=DES-CBC3-SHA bits=168 verify=NOT) 6, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 13:35:48 -0700 6, 25 -- Message-Id: {6.1.2.0.2.20050726133320.027a6f90-at-mail.ucla.edu} 6, 25 -- X-Sender: sryazant-at-mail.ucla.edu 6, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 25 -- Date: Tue, 26 Jul 2005 13:35:30 -0700 6, 25 -- To: microscopy-at-microscopy.com 6, 25 -- From: Sergey {sryazant-at-ucla.edu} 6, 25 -- Subject: Re: [Microscopy] sorry about that 6, 25 -- In-Reply-To: {200507261653.j6QGrRYO006373-at-ns.microscopy.com} 6, 25 -- References: {200507261653.j6QGrRYO006373-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 25 -- X-Probable-Spam: no 6, 25 -- X-Scanned-By: smtp.ucla.edu on 169.232.48.138 ==============================End of - Headers==============================
And I wouldn't take much (any) notice of postings by anyone who doesn't identify themselves but who hides behind a freebie email address
cheers
rtch
Date sent: Tue, 26 Jul 2005 15:37:50 -0500 To: r.sims-at-auckland.ac.nz X-from: maureen_petersen-at-msn.com Send reply to: microscopy-at-microscopy.com
--- r.sims-at-auckland.ac.nz wrote: } And I wouldn't take much (any) notice of postings by } anyone who doesn't identify } themselves but who hides behind a freebie email } address } } cheers } } rtch
If this were a totally "private" list, I would agree with you. But given that it is realatively open, and given the current climate, from a corporate, identity theft, legal "this opinion is not necess....", etc., I don't know that it's fair to just write off a poster because they choose not to be fully identified.
John W. Raffensperger, Jr. Beaver Dam, Wisconsin, USofA
Ditto. It was funny and appreciated. I shared it with several friends. Thanks for posting. -Angela
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Don't be sorry: it was good posting and we need something humorous here } from time to time. Sergey } } At 09:53 AM 7/26/2005, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America
-- Angela V. Klaus, Ph.D. Director, Microscopy and Imaging Facility American Museum of Natural History Central Park West and 79th Street New York, NY 10024 USA Email: avklaus-at-amnh.org Tel: 212-796-5977 Fax: 212-496-3480
==============================Original Headers============================== 5, 32 -- From avklaus-at-amnh.org Tue Jul 26 16:23:39 2005 5, 32 -- Received: from lepore.amnh.org (lepore.amnh.org [216.73.241.12]) 5, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QLNd1Y030792 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 16:23:39 -0500 5, 32 -- Received: from localhost (localhost [127.0.0.1]) 5, 32 -- by lepore.amnh.org (Postfix) with ESMTP id 445995B776 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 17:23:38 -0400 (EDT) 5, 32 -- Received: from lepore.amnh.org ([127.0.0.1]) 5, 32 -- by localhost (lepore.amnh.org [127.0.0.1]) (amavisd-new, port 10024) 5, 32 -- with ESMTP id 00917-05; Tue, 26 Jul 2005 17:23:34 -0400 (EDT) 5, 32 -- Received: from webmail.amnh.org (abel.amnh.org [216.73.241.18]) 5, 32 -- by lepore.amnh.org (Postfix) with ESMTP id 2FFC35B768; 5, 32 -- Tue, 26 Jul 2005 17:23:34 -0400 (EDT) 5, 32 -- Received: from 216.226.38.2 5, 32 -- (SquirrelMail authenticated user avklaus) 5, 32 -- by webmail.amnh.org with HTTP; 5, 32 -- Tue, 26 Jul 2005 17:23:34 -0400 (EDT) 5, 32 -- Message-ID: {63240.216.226.38.2.1122413014.squirrel-at-webmail.amnh.org} 5, 32 -- In-Reply-To: {200507262036.j6QKa4jw031745-at-ns.microscopy.com} 5, 32 -- References: {200507262036.j6QKa4jw031745-at-ns.microscopy.com} 5, 32 -- Date: Tue, 26 Jul 2005 17:23:34 -0400 (EDT) 5, 32 -- Subject: Re: [Microscopy] Re: sorry about that 5, 32 -- From: "Angela V. Klaus" {avklaus-at-amnh.org} 5, 32 -- To: microscopy-at-microscopy.com 5, 32 -- Cc: avklaus-at-amnh.org 5, 32 -- User-Agent: SquirrelMail/1.4.4 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Type: text/plain;charset=iso-8859-1 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-Priority: 3 (Normal) 5, 32 -- Importance: Normal 5, 32 -- X-Virus-Scanned: amavisd-new at amnh.org ==============================End of - Headers==============================
There was no problem with Beth's initial posting and no apology was needed here Beth. Sorry if I appeared to have come down on you.
The followups IMHO were starting to drift into critiques of Marilyn whomever and her "crap Journalism" and I was trying to nip that direction of critique in the bud. However, I see I had the opposite effect. Oh well.....
As Dorrance said, everyone can use a grin occassionally .
Nestor
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==============================Original Headers============================== 13, 13 -- From zaluzec-at-microscopy.com Tue Jul 26 17:49:32 2005 13, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 13, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QMnUg4007030 13, 13 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 17:49:31 -0500 13, 13 -- Mime-Version: 1.0 13, 13 -- Message-Id: {p06110401bf0c6a18025e-at-[206.69.208.22]} 13, 13 -- In-Reply-To: {200507261651.j6QGpWZD000477-at-ns.microscopy.com} 13, 13 -- References: {200507261651.j6QGpWZD000477-at-ns.microscopy.com} 13, 13 -- Date: Tue, 26 Jul 2005 17:49:30 -0500 13, 13 -- To: microscopy-at-microscopy.com 13, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 13, 13 -- Subject: Administrivia: sorry about that/most bright people 13, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Nestor I don't see any reason why we could not discuss the quality of somebody's work even if it's a journalist with former (?) high IQ. Is it prohibited to discuss people's work with high IQ on this ListServer? Sergey
At 03:51 PM 7/26/2005, you wrote:
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_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry and UCLA Institute of Nanotechnology 10833 Le Conte Ave, Room 33-080 Los Angeles, CA 90095
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, July 26, 2005 at 18:41:08 ---------------------------------------------------------------------------
Email: cornheadorama-at-hotmail.com Name: Jeff Miller
Organization: Techutopia
Title-Subject: [Filtered] Looking for limited TAS(+?) motor wiring information.
Question: Hi,
I have a Leitz Orthoplan microscope from a TAS (or is it TAS+?) system. It has motorized stage and fine focus, relay lenses etc. for video.
I didn't pick up the console, controller, or schematics. I'm getting pretty handy with LabVIEW machine vision and motion control, so I'd like to revive this scope around a PC platform.
I need limited wiring diagram information. In particular, I need pinouts for the stepper motors, or to figure out the pinouts indirectly from other elements of the schematic. Ratings or replacement part information for the motors might be helpfull too, barring that I may be able to approximate these by studying the schematic in more detail.
If you have any wiring information for these things, please let me know.
} From the number of pins present, it appears as though the X and Y drives may have encoders associated with them, wheras focus deson't. I don't have the image rotation prism option, and in fact I'd be passively interested in buying one (image roatation is pretty trivial in SW these days).
I'd also be willing to part with the 'scope as opposed to undertaking the project if you're a big fan, and if I do proceed with the PC retrofit I may be offering the whole package after I've played with it for a while. The real motivation for having it evaporated soon after I bought it several years ago.
A honest discussion or even disagreement about a subject or work which is microscopy related (even tangentially) is not prohibited.
However, it is against the rules to intentionally disparage any person or organization on the Listserver. This rule applies equally to Journalists as well as Microscopists. Please review the rules which you received upon subscription.
Presumably a person of hi IQ has the ability to distinguish between these two.
Nestor Your Friendly Neighborhood SysOp
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==============================Original Headers============================== 15, 13 -- From zaluzec-at-microscopy.com Tue Jul 26 19:09:12 2005 15, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 15, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6R09BwQ032014 15, 13 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 19:09:11 -0500 15, 13 -- Mime-Version: 1.0 15, 13 -- Message-Id: {p06110405bf0c7c0b3751-at-[206.69.208.22]} 15, 13 -- In-Reply-To: {200507262340.j6QNeZQI016170-at-ns.microscopy.com} 15, 13 -- References: {200507262340.j6QNeZQI016170-at-ns.microscopy.com} 15, 13 -- Date: Tue, 26 Jul 2005 19:09:10 -0500 15, 13 -- To: microscopy-at-microscopy.com 15, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 15, 13 -- Subject: Discussion of high IQ vs Disparagement of a Person 15, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
My IQ is very low, Nestor, so I am electron microscopist for last 30 years... Is against rules to say truth? You know, truth sometime hurts and may be politically incorrect... This forum becomes too much politically correct and heavily censured (yes, it's true), so I am loosing interest here... I had enough censorship and "rules" in USSR. But, I have to admit US is over performing USSR now, so I feel I am at home. Sergey
At 05:10 PM 7/26/2005, you wrote:
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_____________________________________
Sergey Ryazantsev Ph. D. Electron Microscopy UCLA School of Medicine Department of Biological Chemistry and UCLA Institute of Nanotechnology 10833 Le Conte Ave, Room 33-080 Los Angeles, CA 90095
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } My IQ is very low, Nestor, so I am electron } microscopist for last 30 } years... Is against rules to say truth? You know, } truth sometime hurts } and may be politically incorrect... This forum } becomes too much politically } correct and heavily censured (yes, it's true), so I } am loosing interest } here... I had enough censorship and "rules" in } USSR. But, I have to admit } US is over performing USSR now, so I feel I am at } home. Sergey } } } At 05:10 PM 7/26/2005, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Sergey } } } } A honest discussion or even disagreement about a } subject or work which } } is microscopy } } related (even tangentially) is not prohibited. } } } } However, it is against the rules to intentionally } disparage any person } } or organization on the Listserver. This rule } applies equally to } } Journalists as well } } as Microscopists. Please review the rules which } you received } } upon subscription. } } } } } } http://microscopy.com/MicroscopyListserver/Rules.html } } } } Presumably a person of hi IQ has the ability to } distinguish between these } } two. } } } } Nestor } } Your Friendly Neighborhood SysOp } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ---------------------------------------------------------------------------- } } } } } } Nestor } } } I don't see any reason why we could not discuss } the quality of somebody's } } } work even if it's a journalist with former (?) } high IQ. Is it prohibited } } } to discuss people's work with high IQ on this } ListServer? Sergey } } } } } } At 03:51 PM 7/26/2005, you wrote: } } } } } } } } } } } } } } ------------------------------------------------------------------------ } } } ---- } } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ------------------------------------------------------------------------ } } } ---- } } } } } } } } Beth & everyone else } } } } } } } } There was no problem with Beth's initial } posting and no apology } } } } was needed here Beth. Sorry if I appeared to } have come down on you. } } } } } } } } The followups IMHO were starting to drift into } critiques of Marilyn } } whomever } } } } and her "crap Journalism" and I was trying to } nip that direction of } } } } critique in the bud. However, I see I had the } opposite effect. Oh well..... } } } } } } } } As Dorrance said, everyone can use a grin } occassionally . } } } } } } } } } } } } Nestor } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------- } } } ------ } } } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of } } America } } } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------- } } } ------ } } } } } } } } } } I apologize to the list for posting Marilyn } vos Savant's statement on } } } } } microscopy. I just wanted to share the article } because I thought it was } } } } } humorous...I shall refrain from such postings. } } } } } best, } } } } } Beth } } } } } } } } } } } ********************************************************************** } } } } } Beth Richardson } } } } } EM Lab Coordinator } } } } } Plant Biology Department } } } } } University of Georgia } } } } } Athens, GA 30602-7271 } } } } } } } } } } Phone - (706) 542-1790 & FAX - (706) } 542-1805 } } } } } http://www.plantbio.uga.edu/emlab } } } } } } } } } } "Between the two evils, } } } } } I always pick the one I never tried before". } Mae West (1893-1980) } } } } } } ******************************************************************* } } } } } } } } } } "And it's only the giving that makes you what } you are". } } } } } Wond'ring Aloud, Jethro Tull (Aqualung) } } } } } } } } } } } ************************************************************************ } } } } } *** } } } } } } } } } } } } } } } } } } } } ==============================Original } } Headers============================== } } } } } 8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26 } 11:51:32 2005 } } } } } 8, 18 -- Received: from } dogwood.plantbio.uga.edu } } } } (dogwood.plantbio.uga.edu [128.192.26.2]) } } } } } 8, 18 -- by ns.microscopy.com } (8.12.11/8.12.8) with ESMTP id } } } } j6QGpVhb000423 } } } } } 8, 18 -- for } {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 } } } } 11:51:31 -0500 } } } } } 8, 18 -- Received: from localhost } ([127.0.0.1]) } === message truncated ===
____________________________________________________ Start your day with Yahoo! - make it your home page http://www.yahoo.com/r/hs
==============================Original Headers============================== 6, 19 -- From hoffpajo-at-yahoo.com Tue Jul 26 20:30:51 2005 6, 19 -- Received: from web50202.mail.yahoo.com (web50202.mail.yahoo.com [206.190.38.43]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6R1Up45027111 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 20:30:51 -0500 6, 19 -- Received: (qmail 91470 invoked by uid 60001); 27 Jul 2005 01:30:50 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 19 -- b=0X15dlNJLlpVK4Y9tuofD9U/i1MLd2U2YVPuQS5kZ9PMXmcvK3L04ITxP1vZt+ySqS+dGGdJsVOKF1au0QSUCRdXFIgRayAk4+gLjek7Rhdx1OmBvcGXXRWkSfahwyUcZZJetlKn+eUqv9wkGl78CWrDaL6XY8pMCXNLvsmkwr0= ; 6, 19 -- Message-ID: {20050727013050.91468.qmail-at-web50202.mail.yahoo.com} 6, 19 -- Received: from [68.32.53.160] by web50202.mail.yahoo.com via HTTP; Tue, 26 Jul 2005 18:30:50 PDT 6, 19 -- Date: Tue, 26 Jul 2005 18:30:50 -0700 (PDT) 6, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com} 6, 19 -- Subject: Re: [Microscopy] Re: Discussion of high IQ vs Disparagement of a 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- In-Reply-To: {200507270129.j6R1Tf7M024770-at-ns.microscopy.com} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Great posting. One has to wonder about the role model of ethical values when one bases their decisions on the basis of what they can "get away " with.
You probably think I am losing my balls for keeping silent. I almost composed a posting but then again, I did not want to antagonize some of my best customers. Which is of course exactly what I woudl be doing.
Maybe I will have some scotch and get up the nerve to create a posting. Question: Will we see you in Honolulu?
Chuck ----- Original Message ----- X-from: {donc-at-asmicro.com} To: {cgarber-at-2spi.com} Sent: Tuesday, July 26, 2005 12:52 PM
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
Please accept my apologies for inadvertently sending out a message that was going to an individual and was obviously not intended for the list at large.
I am embarrassed about this and will try to make sure this never happens again.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 7, 13 -- From cgarber-at-2spi.com Tue Jul 26 21:51:09 2005 7, 13 -- Received: from sccrmhc12.comcast.net (sccrmhc12.comcast.net [204.127.202.56]) 7, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6R2p9Eo003595 7, 13 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 26 Jul 2005 21:51:09 -0500 7, 13 -- Message-Id: {200507270251.j6R2p9Eo003595-at-ns.microscopy.com} 7, 13 -- Received: from [127.0.0.1] (pcp02988842pcs.malvrn01.pa.comcast.net[68.85.250.247]) 7, 13 -- by comcast.net (sccrmhc12) with SMTP 7, 13 -- id {20050727025107012009vmd7e} ; Wed, 27 Jul 2005 02:51:07 +0000 7, 13 -- To: MICROSCOPY BB {Microscopy-at-msa.microscopy.com} 7, 13 -- Subject: Apology to the list 7, 13 -- Date: Tue, 26 Jul 2005 22:51:18 -0500 7, 13 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 7, 13 -- X-Mailer: E-Mail Connection v3.1a ==============================End of - Headers==============================
It's OK Chuck, Scotch can do this to you! ;-) Markus ----- Original Message ----- X-from: {cgarber-at-2spi.com} To: {micro-at-superlink.net} Sent: Tuesday, July 26, 2005 10:51 PM
Sergy
I don't care who you are or where your from, but I insist the rules which have been established for over a decade are followed by all. Since you believe you are above the rules so be it. You are welcome to go elsewhere.
I have canceled your subscription to the Listserver.
Are you thinking of having a Saturday newsletter? There was one in Savannah... I don't critical.
My cell phone number is 808-428-7546
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 20 -- From tina-at-pbrc.hawaii.edu Tue Jul 26 23:31:25 2005 6, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6R4VP3Z029164 6, 20 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 23:31:25 -0500 6, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id j6R4VKuX012760 6, 20 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 6, 20 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 18:31:21 -1000 (HST) 6, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id j6R4VJsc012757 6, 20 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 18:31:20 -1000 (HST) 6, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 20 -- Date: Tue, 26 Jul 2005 18:31:19 -1000 (HST) 6, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 20 -- X-Sender: tina-at-halia 6, 20 -- To: microscopy-at-microscopy.com 6, 20 -- Subject: Daily newsletter 6, 20 -- In-Reply-To: {200507261710.j6QHAVTW000305-at-ns.microscopy.com} 6, 20 -- Message-ID: {Pine.GSO.4.21.0507261830040.12448-100000-at-halia} 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Yikes! I laugh when people inadvertantly post to the entire List, and now I've done it! Sorry! And missing a couple of words, too. I'm tired...
See many of you in Honolulu next week!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 19 -- From tina-at-pbrc.hawaii.edu Tue Jul 26 23:37:38 2005 6, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6R4bbsl004449 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 26 Jul 2005 23:37:37 -0500 6, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id j6R4bXJj012807 6, 19 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 26 Jul 2005 18:37:33 -1000 (HST) 6, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id j6R4bWIY012804 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 26 Jul 2005 18:37:32 -1000 (HST) 6, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 19 -- Date: Tue, 26 Jul 2005 18:37:31 -1000 (HST) 6, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 19 -- X-Sender: tina-at-halia 6, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 6, 19 -- Subject: Sorry for mass post 6, 19 -- Message-ID: {Pine.GSO.4.21.0507261835250.12448-100000-at-halia} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
No apology needed. There are laws and ethics that we overtly or tacitly agree to abide by. If there is language in a contract, regulation or law then we would all like to believe that our peers actually abide by those terms.
We all have financial issues to contend with and I would hope our community conducts it business/research/education with respect and fairness to others. If an NSF grant or monies require directing a commercial service AFM inquiry to a service lab in Indiana, then it should be done without question. It is a moral and legal obligation. No excuses just as that Indiana lab is not excused from paying taxes which in turn supports those grants and institutions.
Alan Stone
At 09:52 PM 7/26/2005, you wrote:
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Alan Stone ASTON Metallurgical Services Co., Inc. 200 Larkin Drive Ste A Wheeling, IL 60090 847/353-8100 www.astonmet.com
==============================Original Headers============================== 11, 17 -- From as-at-astonmet.com Wed Jul 27 07:41:30 2005 11, 17 -- Received: from outbound4.mail.tds.net (outbound4.mail.tds.net [216.170.230.94]) 11, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RCfUUd024889 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 07:41:30 -0500 11, 17 -- Received: from AlansXPS.astonmet.com (vnhlilckedg01-xdata1-a4.vnhlil.tds.net [69.11.219.4]) 11, 17 -- by outbound4.mail.tds.net (8.13.4/8.12.2) with ESMTP id j6RCfRqs004114 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 07:41:29 -0500 (CDT) 11, 17 -- Message-Id: {6.2.0.14.2.20050727071540.025993c8-at-pop.tds.net} 11, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 11, 17 -- Date: Wed, 27 Jul 2005 07:41:27 -0500 11, 17 -- To: microscopy-at-microscopy.com 11, 17 -- From: Alan Stone {as-at-astonmet.com} 11, 17 -- Subject: Re: [Microscopy] Apology to the list 11, 17 -- In-Reply-To: {200507270252.j6R2qIra006130-at-ns.microscopy.com} 11, 17 -- References: {200507270252.j6R2qIra006130-at-ns.microscopy.com} 11, 17 -- Mime-Version: 1.0 11, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Nestor: I would respectfully ask you to reconsider dropping Sergey from the list. His comment was not that egrious. He is a very active member of this listserver and it would be a shame to drop him. I have never met him and this comment is not motivated by friendship or a personal tie. Tom Phillips
At 10:03 PM 07/26/05, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I was frankly relieved to see you step in a couple of times on this one, and your reminder to everyone to review the rules is obviously necessary. (I had already pulled them out myself yesterday, as a check against some of what was being posted.)
I was surprised by the tone and the subjectivity of some of the initial responses to what was obviously an amusing posting, and people do need to be reminded that the listserver is an educational/informational exchange, rather than a personal soapbox. I don't think it's an accident that the words, 'not a right, but a privilege', show up more than once in the rules.
Keep up the good work!
See you next week,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
-----Original Message----- X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] Sent: Tuesday, July 26, 2005 10:03 PM To: Elaine F. Schumacher
Sergy
I don't care who you are or where your from, but I insist the rules which have been established for over a decade are followed by all. Since you believe you are above the rules so be it. You are welcome to go elsewhere.
I have canceled your subscription to the Listserver.
I've gotten several questions lately on why things are operating slighlty differently recently. Particuliarly with respect to replies. If your interested please read on otherwise just trash this.
As you know SPAM has grown enormously this list gets "attacked" daily. The rate is approaching 1000 spam messages per day. Most of these were stopped but about one or two every week managed to get through.
What you MAY NOT know is that every message that is rejected by the listserver software gets a reply sent to the originator, letting them know there was a problem and how to deal with it. A number of you occassional get this message when there a problem with things you try to post. However on average this is a low number.
What you DEFINITELY don't know is that alot of the SPAM addresses are bogus and various Email servers just return the "There is a problem with your Email" message as undeliverable. Now think for a minute these messages are returned to someone and guess who that is....
Yep I've get barraged by huge numbers of not only spam but also literally hundreds of rejected mail messages the number of which grows daily. Being somewhat consciencous I check each just in case it was a real subscriber that had a problem.
The long and short of it was that things were just getting unmanagable, and I had to redesign the filter software. This has reduced the probem from 750+ junk messages/day to 1 or 2. The unfortunate thing is that now every message has a REPLY line which say microscopy listserver and it has a FROM line indicating the originator of the Email.
The net result of this is if you REPLY the message goes to the list. If you want to send a private message you will need to copy and paste the Email address of the sender into a new Email message. At the moment that is the correct procedure.
There are pro's and con's of this, the advantage is that it has made my life alot less annoying when I get home in the evening and only have to deal with a few problems. The Con, is the occassional, inadvertently posted message.
I'll pontificate abit on this abit longer and try to figure out a solution to the problem, which is compatible with both my needs to occassionally have a real life out side of this electronic world, and the convenience of replying . To be honest, I have lots to do right now and this is not a simple issue as there are lots of hidden loop holes which need to be managed.
For the moment just try to pay close attention. Whenever you hit reply look at the "TO" address , the location where a message is going is displayed on you Email and is never hidden by any good Email client program.
Nestor Your Tired Friendly (?) Neighborhood SysOp
==============================Original Headers============================== 14, 11 -- From zaluzec-at-microscopy.com Wed Jul 27 08:35:03 2005 14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 14, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RDZ3u7015953 14, 11 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 08:35:03 -0500 14, 11 -- Mime-Version: 1.0 14, 11 -- Message-Id: {p06110404bf0d359eb690-at-[206.69.208.22]} 14, 11 -- Date: Wed, 27 Jul 2005 08:35:02 -0500 14, 11 -- To: microscopy-at-microscopy.com 14, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 14, 11 -- Subject: Administrivia: Posting to the List vs Replying 14, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Thank you, Nestor. I really hope this thread will die now.
Geoff
zaluzec-at-microscopy.com wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 31 -- From mcauliff-at-umdnj.edu Wed Jul 27 08:43:16 2005 8, 31 -- Received: from mail01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 8, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RDhGtH023815 8, 31 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 08:43:16 -0500 8, 31 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 8, 31 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 19304EC062 8, 31 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 09:43:16 -0400 (EDT) 8, 31 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 8, 31 -- by mail01.umdnj.edu (Proprietary) with ESMTP id E00E8EC053 8, 31 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 09:43:14 -0400 (EDT) 8, 31 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 8, 31 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 8, 31 -- id {0IKA00801G9QOP-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 8, 31 -- for microscopy-at-microscopy.com; Wed, 27 Jul 2005 09:43:14 -0400 (EDT) 8, 31 -- Received: from [127.0.0.1] ([10.138.2.240]) 8, 31 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 8, 31 -- 2004)) with ESMTP id {0IKA008IEGN8ZS-at-Polaris.umdnj.edu} for 8, 31 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 09:40:20 -0400 (EDT) 8, 31 -- Date: Wed, 27 Jul 2005 09:40:19 -0400 8, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 31 -- Subject: Re: [Microscopy] Administrivia: Rules 8, 31 -- In-reply-to: {200507270303.j6R33E96021281-at-ns.microscopy.com} 8, 31 -- To: microscopy-at-microscopy.com 8, 31 -- Message-id: {42E78EC3.8080805-at-umdnj.edu} 8, 31 -- MIME-version: 1.0 8, 31 -- Content-type: text/plain; format=flowed; charset=us-ascii 8, 31 -- Content-transfer-encoding: 7BIT 8, 31 -- X-Accept-Language: en-us, en 8, 31 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 8, 31 -- Gecko/20040804 Netscape/7.2 (ax) 8, 31 -- References: {200507270303.j6R33E96021281-at-ns.microscopy.com} ==============================End of - Headers==============================
I was enjoying the IQ discourse when it was light-hearted, but I don't believe the Microscopy Listserver, or any other professional listserver, should disparage people personally. It is not a question of censorship but of human decency. What is it our parents said? "If you can't say something nice about someone then don't say anything at all". The IQ lady publicly wrote her opinion, so that leaves those words as open game for criticism. I think it is unfortunate that Sergey was not allowed to make his own choice about leaving the list; in that way, he was censored. This is my personal opinion (and since I submitted it, you folks are all welcome to criticize it.)
Jane L. LaGoy R&D Engineer Bodycote HIP 155 River Street Andover, MA 01810
==============================Original Headers============================== 3, 15 -- From Jane.LaGoy-at-bodycote.com Wed Jul 27 08:47:35 2005 3, 15 -- Received: from Exchange.BODYCOTE-IMT.COM (mail.bodycote-imt.com [12.30.23.178]) 3, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RDlYof028763 3, 15 -- for {Microscopy-at-MSA.Microscopy.com} ; Wed, 27 Jul 2005 08:47:35 -0500 3, 15 -- Received: by mail.bodycote-imt.com with Internet Mail Service (5.5.2657.72) 3, 15 -- id {3Y5JKY1V} ; Wed, 27 Jul 2005 09:48:37 -0400 3, 15 -- Message-ID: {CBB9714FDC67D411B39400D0B73C4B7302336FD8-at-mail.bodycote-imt.com} 3, 15 -- From: JLaGoy {Jane.LaGoy-at-bodycote.com} 3, 15 -- To: "Microscopy Listserver (E-mail)" {Microscopy-at-MSA.Microscopy.com} 3, 15 -- Subject: my $0.02 worth on disparagement 3, 15 -- Date: Wed, 27 Jul 2005 09:48:34 -0400 3, 15 -- MIME-Version: 1.0 3, 15 -- X-Mailer: Internet Mail Service (5.5.2657.72) 3, 15 -- Content-Type: text/plain; 3, 15 -- charset="iso-8859-1" ==============================End of - Headers==============================
I would back this opinion. Especially since the line: "Presumably a person of hi IQ has the ability to distinguish between these two" in Nestor's email could have been misinterpreted by Sergey as an unwarranted and disparaging remark on his IQ level. At least, this is the way I understood it, and apparently Sergey too. I find this very disturbing...
Marc
On Wednesday, July 27, 2005, at 09:15 AM, phillipst-at-missouri.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Nestor: I would respectfully ask you to reconsider dropping Sergey } from the } list. His comment was not that egrious. He is a very active member of } this } listserver and it would be a shame to drop him. I have never met him } and } this comment is not motivated by friendship or a personal tie. Tom } Phillips } } At 10:03 PM 07/26/05, you wrote: } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } Sergy } } } } I don't care who you are or where your from, but I insist the rules } } which have } } been established for over a decade are followed by all. Since you } } believe you are above the rules so be it. You are welcome to } } go elsewhere. } } } } I have canceled your subscription to the Listserver. } } } } } } Nestor } } Microscopy SysOp } } } } } } } } ==============================Original } } Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Tue Jul 26 22:02:16 2005 } } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } j6R32FsY019078 } } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 } } 22:02:16 } } -0500 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: {p0611040cbf0ca908c2d4-at-[206.69.208.22]} } } 7, 11 -- Date: Tue, 26 Jul 2005 22:02:14 -0500 } } 7, 11 -- To: microscopy-at-microscopy.com } } 7, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} } } 7, 11 -- Subject: Administrivia: Rules } } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } } ==============================End of - } } Headers============================== } } Thomas E. Phillips, PhD } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } } 573-882-4712 (office) } 573-882-0123 (fax) } PhillipsT-at-missouri.edu } } } } ==============================Original } Headers============================== } 9, 20 -- From PhillipsT-at-missouri.edu Wed Jul 27 08:14:54 2005 } 9, 20 -- Received: from um-exproto9.um.umsystem.edu } (um-exproto9.um.umsystem.edu [207.160.151.49]) } 9, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6RDEsRj000417 } 9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 08:14:54 } -0500 } 9, 20 -- Received: from um-exproto9.um.umsystem.edu } ([207.160.151.148]) by um-exproto9.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.1830); } 9, 20 -- Wed, 27 Jul 2005 08:14:53 -0500 } 9, 20 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) } by um-exproto9.um.umsystem.edu over TLS secured channel with Microsoft } SMTPSVC(6.0.3790.1830); } 9, 20 -- Wed, 27 Jul 2005 08:14:52 -0500 } 9, 20 -- Message-Id: } {6.0.0.22.2.20050727081355.01f66bc0-at-pop.missouri.edu} } 9, 20 -- X-Sender: phillipst-at-pop.missouri.edu } 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22 } 9, 20 -- Date: Wed, 27 Jul 2005 08:16:52 -0500 } 9, 20 -- To: microscopy-at-microscopy.com } 9, 20 -- From: Tom Phillips {phillipst-at-missouri.edu} } 9, 20 -- Subject: Re: [Microscopy] Administrivia: Rules } 9, 20 -- In-Reply-To: {200507270303.j6R3335J020764-at-ns.microscopy.com} } 9, 20 -- References: {200507270303.j6R3335J020764-at-ns.microscopy.com} } 9, 20 -- Mime-Version: 1.0 } 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 9, 20 -- X-OriginalArrivalTime: 27 Jul 2005 13:14:52.0698 (UTC) } FILETIME=[2CB4EFA0:01C592AD] } ==============================End of - } Headers============================== } }
-- Marc Pypaert Department of Cell Biology Center for Cell and Molecular Imaging Ludwig Institute for Cancer Research Yale University School of Medicine 333 Cedar Street, PO Box 208002 New Haven, CT 06520-8002 TEL 203-785 3681 FAX 203-785 7446
==============================Original Headers============================== 7, 18 -- From marc.pypaert-at-yale.edu Wed Jul 27 09:36:28 2005 7, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48]) 7, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6REaSob007352 7, 18 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 09:36:28 -0500 7, 18 -- Received: from yale.edu (net234-111.med.yale.edu [130.132.234.111]) 7, 18 -- by biomed.med.yale.edu (PMDF V6.1-1 #30532) 7, 18 -- with ESMTP id {01LR47P1GMOO00BUTS-at-biomed.med.yale.edu} for 7, 18 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 10:31:56 -0400 (EDT) 7, 18 -- Date: Wed, 27 Jul 2005 10:31:34 -0400 7, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu} 7, 18 -- Subject: Re: [Microscopy] Re: Administrivia: Rules 7, 18 -- In-reply-to: {200507271315.j6RDFjJ9002248-at-ns.microscopy.com} 7, 18 -- To: microscopy-at-microscopy.com 7, 18 -- Message-id: {21BD0816-FEAB-11D9-AC3D-0030659833B4-at-yale.edu} 7, 18 -- MIME-version: 1.0 (Apple Message framework v553) 7, 18 -- X-Mailer: Apple Mail (2.553) 7, 18 -- Content-type: text/plain; format=flowed; charset=US-ASCII; delsp=yes 7, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Nestor..........since you just used a derogatory and demeaning statement about someone publically using the listserver........are you going to remove yourself from the list? I think the "I dont care who you are" could have been left off and your point still made.
Nestor wrote: "Sergy
I don't care who you are or where your from, but I insist the rules which have been established for over a decade are followed by all. Since you believe you are above the rules so be it. You are welcome to go elsewhere.
I have canceled your subscription to the Listserver.
Nestor Microscopy SysOp"
==============================Original Headers============================== 8, 14 -- From bmollon-at-pacbell.net Wed Jul 27 09:41:38 2005 8, 14 -- Received: from web81308.mail.yahoo.com (web81308.mail.yahoo.com [206.190.37.83]) 8, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6REfcr9012847 8, 14 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 09:41:38 -0500 8, 14 -- Received: (qmail 17265 invoked by uid 60001); 27 Jul 2005 14:41:38 -0000 8, 14 -- Message-ID: {20050727144138.17263.qmail-at-web81308.mail.yahoo.com} 8, 14 -- Received: from [69.225.7.253] by web81308.mail.yahoo.com via HTTP; Wed, 27 Jul 2005 07:41:38 PDT 8, 14 -- Date: Wed, 27 Jul 2005 07:41:38 -0700 (PDT) 8, 14 -- From: Bill Mollon {bmollon-at-pacbell.net} 8, 14 -- Subject: rules 8, 14 -- To: microscopy-at-microscopy.com 8, 14 -- MIME-Version: 1.0 8, 14 -- Content-Type: text/plain; charset=iso-8859-1 8, 14 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
You do so much for this community and use such an even hand and light touch on this list that I could not believe that he was pushing it (and not for the first time)! You have my full support. I'd post this but I don't want to start/continue a flood!
Cheers, John
On 7/26/05 8:03 PM, "zaluzec-at-microscopy.com" {zaluzec-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Sergy } } I don't care who you are or where your from, but I insist the rules which have } been established for over a decade are followed by all. Since you } believe you are above the rules so be it. You are welcome to } go elsewhere. } } I have canceled your subscription to the Listserver. } } } Nestor } Microscopy SysOp } } } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Tue Jul 26 22:02:16 2005 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6R32FsY019078 } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 22:02:16 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p0611040cbf0ca908c2d4-at-[206.69.208.22]} } 7, 11 -- Date: Tue, 26 Jul 2005 22:02:14 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} } 7, 11 -- Subject: Administrivia: Rules } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
-- ******** John S. Vetrano Sr. Research Scientist Materials Structure and Performance Group Pacific Northwest National Laboratory MSIN P8-16 P.O. Box 999 Richland, WA 99352 Phone: (509)372-0724 Fax: (509)376-6308 Email: mailto:john.vetrano-at-pnl.gov
==============================Original Headers============================== 7, 28 -- From john.vetrano-at-pnl.gov Wed Jul 27 10:37:10 2005 7, 28 -- Received: from atlas.pnl.gov (atlas.pnl.gov [130.20.248.194]) 7, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RFb9vY023582 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:37:09 -0500 7, 28 -- Received: from emailfilter1.pnl.gov (emailfilter1.pnl.gov [130.20.248.23]) 7, 28 -- by pnl.gov (PMDF V6.2 #31042) with ESMTP id {0IKA000IBM1WXC-at-pnl.gov} for 7, 28 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 08:37:09 -0700 (PDT) 7, 28 -- Received: from pnlmse21.pnl.gov (Not Verified[130.20.128.55]) 7, 28 -- by emailfilter1.pnl.gov with NetIQ MailMarshal (v5.5.6.6) 7, 28 -- id {BA013f7cbf} ; Wed, 27 Jul 2005 08:37:10 -0700 7, 28 -- Received: from pnlmse24.pnl.gov ([130.20.128.29]) 7, 28 -- by pnlmse21.pnl.gov with Microsoft SMTPSVC(6.0.3790.211); Wed, 7, 28 -- 27 Jul 2005 08:37:08 -0700 7, 28 -- Received: from 130.20.26.66 ([130.20.26.66]) 7, 28 -- by pnlmse24.pnl.gov ([130.20.128.29]) with Microsoft Exchange Server HTTP-DAV 7, 28 -- ; Wed, 27 Jul 2005 15:36:47 +0000 7, 28 -- Date: Wed, 27 Jul 2005 08:36:47 -0700 7, 28 -- From: "Vetrano, John S" {john.vetrano-at-pnl.gov} 7, 28 -- Subject: Thanks, Nestor 7, 28 -- In-reply-to: {200507270303.j6R3367V020884-at-ns.microscopy.com} 7, 28 -- To: microscopy-at-microscopy.com 7, 28 -- Message-id: {BF0CF81F.76C3%john.vetrano-at-pnl.gov} 7, 28 -- MIME-version: 1.0 7, 28 -- Content-type: text/plain; charset=US-ASCII 7, 28 -- Content-transfer-encoding: 7bit 7, 28 -- User-Agent: Microsoft-Entourage/11.1.0.040913 7, 28 -- X-OriginalArrivalTime: 27 Jul 2005 15:37:08.0741 (UTC) 7, 28 -- FILETIME=[0C95BB50:01C592C1] ==============================End of - Headers==============================
List members et al., Is there any way we can "restore" the system to the point before the I.Q. posting, thereby returning Sergey to the List? I would hate to miss his often insightful comments. I also hate to think that any comment, understood or misunderstood, made in the heat of passion may summarily cause expulsion sans appeal, deservedly or not. "Can we all just get along?!" Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W. Wiggins, E.M. Pathology Asst. CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron Microscopy, LLSPT, A823-A828 8700 Beverly Blvd. Los Angeles, CA 90048 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax) Winston.Wiggins-at-CSHS.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-----Original Message----- X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] Sent: Tuesday, July 26, 2005 5:12 PM To: Winston.Wiggins-at-CSHS.org
Sergey
A honest discussion or even disagreement about a subject or work which is microscopy related (even tangentially) is not prohibited.
However, it is against the rules to intentionally disparage any person or organization on the Listserver. This rule applies equally to Journalists as well as Microscopists. Please review the rules which you received upon subscription.
Presumably a person of hi IQ has the ability to distinguish between these two.
Nestor Your Friendly Neighborhood SysOp
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==============================Original Headers============================== 18, 25 -- From winston.wiggins-at-cshs.org Wed Jul 27 10:46:49 2005 18, 25 -- Received: from csip1.csmc.edu (CSIP1.csmc.edu [192.231.133.35]) 18, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RFkmjF031434 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:46:48 -0500 18, 25 -- Received: from unknown (HELO zixvpm01.csmc.edu) (192.168.10.26) 18, 25 -- by csip1.csmc.edu with ESMTP; 27 Jul 2005 08:46:48 -0700 18, 25 -- X-BrightmailFiltered: true 18, 25 -- X-Brightmail-Tracker: AAAAAA== 18, 25 -- Received: from zixvpm01.csmc.edu (ZixVPM [127.0.0.1]) 18, 25 -- by Outbound.cshs.org (Proprietary) with ESMTP id 2BF4A4BDC8 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:03:55 -0500 (CDT) 18, 25 -- Received: from exchange1.csmc.edu (SMTP.csmc.edu [166.124.43.199]) 18, 25 -- by zixvpm01.csmc.edu (Proprietary) with ESMTP id 0132AA7B6F 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:03:55 -0500 (CDT) 18, 25 -- Received: by SMTP.csmc.edu with Internet Mail Service (5.5.2653.19) 18, 25 -- id {PXCNG821} ; Wed, 27 Jul 2005 08:46:47 -0700 18, 25 -- Message-ID: {B5AEAEDBB5D44C47BFE2A6D791329D7F2ADC7D-at-EXCHANGE24.csmc.edu} 18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} 18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of a Pers 18, 25 -- on 18, 25 -- Date: Wed, 27 Jul 2005 08:46:46 -0700 18, 25 -- MIME-Version: 1.0 18, 25 -- X-Mailer: Internet Mail Service (5.5.2653.19) 18, 25 -- Content-Type: text/plain ==============================End of - Headers==============================
I tend to agree with Winston. This list is too valuable to be torn asunder by one topic and a few heightened emotions. Perhaps when many of us unsubscribe while attending M&M 2005 we can "reboot".
Joseph
Joseph M. Oparowski Center for Materials Science - Consulting and Failure Analysis Bose Corporation Joseph_Oparowski-at-bose.com The Mountain, M/S 415 Phone: (508) 766-1371 Framingham, MA 01701-9168 Fax: (508) 766-1313
-----Original Message----- X-from: Winston.Wiggins-at-cshs.org [mailto:Winston.Wiggins-at-cshs.org] Sent: Wednesday, July 27, 2005 11:50 AM To: Oparowski, Joseph
List members et al., Is there any way we can "restore" the system to the point before the I.Q. posting, thereby returning Sergey to the List? I would hate to miss his often insightful comments. I also hate to think that any comment, understood or misunderstood, made in the heat of passion may summarily cause expulsion sans appeal, deservedly or not. "Can we all just get along?!" Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W. Wiggins, E.M. Pathology Asst. CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron Microscopy, LLSPT, A823-A828 8700 Beverly Blvd. Los Angeles, CA 90048 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax) Winston.Wiggins-at-CSHS.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-----Original Message----- X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] Sent: Tuesday, July 26, 2005 5:12 PM To: Winston.Wiggins-at-CSHS.org
Sergey
A honest discussion or even disagreement about a subject or work which is microscopy related (even tangentially) is not prohibited.
However, it is against the rules to intentionally disparage any person or organization on the Listserver. This rule applies equally to Journalists as well as Microscopists. Please review the rules which you received upon subscription.
Presumably a person of hi IQ has the ability to distinguish between these two.
Nestor Your Friendly Neighborhood SysOp
} ----------------------------------------------------------------------- } ---- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
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If you have received this message in error, please notify us immediately, by calling (310) 423-6428 -- and destroy the related message. Thank You for your cooperation.
==============================Original Headers============================== 18, 25 -- From winston.wiggins-at-cshs.org Wed Jul 27 10:46:49 2005 18, 25 -- Received: from csip1.csmc.edu (CSIP1.csmc.edu [192.231.133.35]) 18, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RFkmjF031434 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:46:48 -0500 18, 25 -- Received: from unknown (HELO zixvpm01.csmc.edu) (192.168.10.26) 18, 25 -- by csip1.csmc.edu with ESMTP; 27 Jul 2005 08:46:48 -0700 18, 25 -- X-BrightmailFiltered: true 18, 25 -- X-Brightmail-Tracker: AAAAAA== 18, 25 -- Received: from zixvpm01.csmc.edu (ZixVPM [127.0.0.1]) 18, 25 -- by Outbound.cshs.org (Proprietary) with ESMTP id 2BF4A4BDC8 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:03:55 -0500 (CDT) 18, 25 -- Received: from exchange1.csmc.edu (SMTP.csmc.edu [166.124.43.199]) 18, 25 -- by zixvpm01.csmc.edu (Proprietary) with ESMTP id 0132AA7B6F 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:03:55 -0500 (CDT) 18, 25 -- Received: by SMTP.csmc.edu with Internet Mail Service (5.5.2653.19) 18, 25 -- id {PXCNG821} ; Wed, 27 Jul 2005 08:46:47 -0700 18, 25 -- Message-ID: {B5AEAEDBB5D44C47BFE2A6D791329D7F2ADC7D-at-EXCHANGE24.csmc.edu} 18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} 18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 18, 25 --
I have to say I was waiting for Chuck's response on this thread - my jaw dropped when I read it, until the explanation came in the next e-mail!!!
More seriously--
Most of the discussion on this thread has been about the harm done to commercial labs by university labs "undercutting", "poaching customers" - or whatever description you want to apply, and by the need to determine the "true" cost of providing the service. I think, though, that there is a serious problem for the university labs themselves in allowing commercial service activities to become an important part of their operation.
Any operation has a core mission. The proprietor of any successful business knows the importance of identifying and maintaining their core mission, and the ex-proprietors of many failed businesses know the costs of straying from the mission. While individual organizations vary, the core mission of a university characterization laboratory is not the same as that of a commercial laboratory. In a university our basic mission is education through the generation of research results; that of the company is to generate a profit through providing an analytical service. These differences, though in some areas subtle, lead to totally different approaches to the way we interact with our clients, the way we manage our instrumentation, our time, etc, etc.
The government (through the NSF, in my case) contributes towards the cost of setting up and maintaining my characterization facilities. If we generate first-class results on new and interesting materials, then this is considered money well-spent. If I dilute this by taking on commercial work, even if this leads to a reduction in the amount of "subsidy", I am reducing the rationale for getting any support, and in general diluting the reputation of my laboratories.
I realize that not everyone in an academic environment has my good fortune to work for an administration that supports the ideas I have just expressed, and I also stress again that we each work in unique environments, but in general I would suggest that in most cases commercial clients are best left to commercial laboratories. A commercial client is generally looking for an analytical service, rather than to be educated.
Tony Garratt-Reed.
At 10:46 PM 7/26/2005, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
*********************************************** Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
I agree, we need to re-boot! It is great place and Sergey's knowledge is needed for us. Thanks, Vlad
At 11:24 AM 7/27/2005 -0500, you wrote:
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==============================Original Headers============================== 8, 21 -- From uti-at-direcpc.com Wed Jul 27 11:32:22 2005 8, 21 -- Received: from a34-mta01.direcway.com (a34-mta01.direcpc.com [66.82.4.90]) 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RGWMkL017513 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 11:32:22 -0500 8, 21 -- Received: from ringo.direcpc.com (dpclt034224.direcpc.com [64.157.34.224]) 8, 21 -- by a34-mta01.direcway.com 8, 21 -- (iPlanet Messaging Server 5.2 HotFix 2.05 (built Mar 3 2005)) 8, 21 -- with ESMTP id {0IKA008U8OLDES-at-a34-mta01.direcway.com} for 8, 21 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 12:32:18 -0400 (EDT) 8, 21 -- Date: Wed, 27 Jul 2005 12:32:18 -0400 8, 21 -- From: UTI {uti-at-direcpc.com} 8, 21 -- Subject: Re: [Microscopy] Discussion of high IQ vs Disparagement of a Pers 8, 21 -- In-reply-to: {200507271624.j6RGO7Fe014123-at-ns.microscopy.com} 8, 21 -- X-Sender: uti-at-pop3.direcpc.com 8, 21 -- To: microscopy-at-microscopy.com 8, 21 -- Cc: sryazant-at-ucla.edu 8, 21 -- Message-id: {5.1.0.14.2.20050727123005.01d97620-at-pop3.direcpc.com} 8, 21 -- MIME-version: 1.0 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 5.1 8, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed 8, 21 -- Content-transfer-encoding: 7BIT ==============================End of - Headers==============================
Folks, lets remenber the Golden Rule with our dealing with the list server....
The man with the gold get to make the rules. Simple is it not?
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you.
==============================Original Headers============================== 12, 17 -- From frank.karl-at-degussa.com Wed Jul 27 11:46:02 2005 12, 17 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 12, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RGjvV1030393 12, 17 -- for {microscopy-at-msa.microscopy.com} ; Wed, 27 Jul 2005 11:46:02 -0500 12, 17 -- Received: from mobuscomm01.mail.degussa.com ([172.20.6.74]) 12, 17 -- by framailout1.rz.itson.com (8.13.3/8.13.3/Debian-6) with ESMTP id j6RGiW5s027918 12, 17 -- for {microscopy-at-msa.microscopy.com} ; Wed, 27 Jul 2005 18:45:20 +0200 12, 17 -- Subject: golden rule applies here 12, 17 -- To: microscopy-at-msa.microscopy.com 12, 17 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 12, 17 -- Message-ID: {OF7928F80A.6C45314D-ON8525704B.005DDFD5-8525704B.005E1722-at-degussa.com} 12, 17 -- From: frank.karl-at-degussa.com 12, 17 -- Date: Wed, 27 Jul 2005 13:07:43 -0400 12, 17 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 12, 17 -- 07/27/2005 11:45:41 AM 12, 17 -- MIME-Version: 1.0 12, 17 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Anybody wishing to integrate quantitative analysis by design-based stereology into his/her studies might find this interesting. Please don´t hesitate to contact me for further details.
Best regards, Matthias
WORKSHOP ON RECENT STEREOLOGY - 19-23 September 2005, Institute of Anatomy, University of Bern, Switzerland.
The workshop is intended for students and researchers from the field of biology, medicine, and material sciences, interested in the theory and practice of geometric sampling and measurement of 3D structures at the LM and EM level.
The approach of the workshop is problem-based; ideally the participants will bring questions and unsolved case studies, and the teachers will try to supply concrete and detailed solutions, eventually explaining the underlying theory in short ad-hoc lectures. No strong background in mathematics or statistics is assumed. Central concepts of geometric sampling and estimation will be introduced at the beginning. Sampling protocols are also demonstrated on real tissue in several Laboratory sessions.
Instructors: L.M. Cruz-Orive, M. Geiser, M. Ochs. Details and registration: http://www.ana.unibe.ch/event/ster/index.html
Matthias Ochs, M.D. Institute of Anatomy Experimental Morphology Unit University of Bern Baltzerstr. 2 CH-3000 Bern 9 Switzerland Phone: +41 31 631 4624 Fax: +41 31 631 3807 E-mail: ochs-at-ana.unibe.ch
==============================Original Headers============================== 14, 19 -- From mochs-at-gwdg.de Wed Jul 27 11:54:31 2005 14, 19 -- Received: from mailer.gwdg.de (mailer.gwdg.de [134.76.10.26]) 14, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RGsU5s005737 14, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 11:54:31 -0500 14, 19 -- Message-Id: {200507271654.j6RGsU5s005737-at-ns.microscopy.com} 14, 19 -- Received: from mailbox.gwdg.de ([134.76.10.21] helo=gwdg.de) 14, 19 -- by mailer.gwdg.de with esmtp (Exim 4.42) 14, 19 -- id 1DxpAs-0001nx-8z 14, 19 -- for Microscopy-at-microscopy.com; Wed, 27 Jul 2005 18:54:30 +0200 14, 19 -- Content-Type: text/plain; charset=iso-8859-1 14, 19 -- X-Originating-IP: mochs[130.92.9.58] 14, 19 -- Date: Wed, 27 Jul 2005 18:54:30 +0200 14, 19 -- MIME-Version: 1.0 14, 19 -- Subject: Course announcement: Workshop on recent stereology 14, 19 -- Content-Transfer-Encoding: 8bit 14, 19 -- User-Agent: IMHO/0.98.3+G (Webmail for Roxen) 14, 19 -- From: Matthias Ochs {mochs-at-gwdg.de} 14, 19 -- To: Microscopy-at-microscopy.com 14, 19 -- X-Virus-Scanned: (clean) by exiscan+sophie ==============================End of - Headers==============================
Well, now that hopefully everyone is finished beating me down and questioning my ethics, I'd like to offer a bit more of an explanation into the reason for my posted question.
First of all, my facility exists to serve my departments needs and those needs alone. The "outsider" in question is a former student who works at another non-profit and who is establishing a pedagogical relationship with us which will include teaching, mentoring, and graduate student research. This person simply asked about providing monetary compensation if the need arose. I was at a loss for how to answer and so thought I would ask the listserv about this.
This is not a situation in which private labs are being undercut or a situation in which an academic lab is pursing commercial sources of funding.
Thanks to Debby for her instructive post on an appropriate methodology for arriving at a cost structure.
AM
-----Original Message----- X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU] Sent: Wednesday, July 27, 2005 12:34 PM To: mccaulak-at-wfu.edu
I have to say I was waiting for Chuck's response on this thread - my jaw dropped when I read it, until the explanation came in the next e-mail!!!
More seriously--
Most of the discussion on this thread has been about the harm done to commercial labs by university labs "undercutting", "poaching customers" - or whatever description you want to apply, and by the need to determine the "true" cost of providing the service. I think, though, that there is a serious problem for the university labs themselves in allowing commercial service activities to become an important part of their operation.
Any operation has a core mission. The proprietor of any successful business knows the importance of identifying and maintaining their core mission, and the ex-proprietors of many failed businesses know the costs of straying from the mission. While individual organizations vary, the core mission of a university characterization laboratory is not the same as that of a commercial laboratory. In a university our basic mission is education through the generation of research results; that of the company is to generate a profit through providing an analytical service. These differences, though in some areas subtle, lead to totally different approaches to the way we interact with our clients, the way we manage our instrumentation, our time, etc, etc.
The government (through the NSF, in my case) contributes towards the cost of setting up and maintaining my characterization facilities. If we generate first-class results on new and interesting materials, then this is considered money well-spent. If I dilute this by taking on commercial work, even if this leads to a reduction in the amount of "subsidy", I am reducing the rationale for getting any support, and in general diluting the reputation of my laboratories.
I realize that not everyone in an academic environment has my good fortune to work for an administration that supports the ideas I have just expressed, and I also stress again that we each work in unique environments, but in general I would suggest that in most cases commercial clients are best left to commercial laboratories. A commercial client is generally looking for an analytical service, rather than to be educated.
Tony Garratt-Reed.
At 10:46 PM 7/26/2005, you wrote:
} --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
*********************************************** Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
I have a small lab, we do mostly basic EM type work.
Now a days, we don't do much sectioning for the TEM. Once and a while someone wants me to do some sectioning, but many weeks (months) may pass between requests.
I am trying to figure out a way to manage the post staining solutions so I don't have to mix them up every time and/or how to make up just a little for the occasional job.
Any neat tricks or systems for storing post stains like lead citrate and uranyl acetate out there?
How about storage life time and/or ideas about mixing minimum quantities.
Thanks
Jon
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
==============================Original Headers============================== 11, 17 -- From jmkrupp-at-cats.ucsc.edu Wed Jul 27 12:23:06 2005 11, 17 -- Received: from cats-mx3.ucsc.edu (cats-mx3.ucsc.edu [128.114.125.36]) 11, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RHN6YI021563 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 12:23:06 -0500 11, 17 -- Received: from [128.114.25.223] (dhcp-25-223.ucsc.edu [128.114.25.223]) 11, 17 -- by cats-mx3.ucsc.edu (8.13.1/8.13.1) with SMTP id j6RHLeke021582 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:21:43 -0700 (PDT) 11, 17 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu 11, 17 -- Message-Id: {v01550100bf0d718a5ef0-at-[128.114.25.150]} 11, 17 -- Mime-Version: 1.0 11, 17 -- Content-Type: text/plain; charset="us-ascii" 11, 17 -- Date: Wed, 27 Jul 2005 10:20:59 -0700 11, 17 -- To: microscopy-at-microscopy.com 11, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) 11, 17 -- Subject: stain solutions 11, 17 -- X-UCSC-CATS-MailScanner: Found to be clean 11, 17 -- X-UCSC-CATS-MailScanner-SpamCheck: ==============================End of - Headers==============================
One issue on this topic is that those of us at state institutions have an obligation to serve the taxpayers of our state as well as the scientists on our respective campuses. Agricultural extension services are an example of organized fulfillment of such obligations. Many of their services are absolutely free. When external customers come to us they are more often looking for help with the science behind their projects rather than merely an analytical service. When their tax dollars support our salaries and other costs, they can certainly expect a break. These relationships could be interpreted as contracts-for-research, which are perfectly legitimate arrangements.
Greg Erdos
tonygr-at-MIT.EDU wrote:
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-- Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 Phone: 352-392-1295 Fax: 352 846 0251
==============================Original Headers============================== 8, 25 -- From gwe-at-ufl.edu Wed Jul 27 12:25:46 2005 8, 25 -- Received: from smtp.ufl.edu (sp42en1.nerdc.ufl.edu [128.227.74.42]) 8, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RHPk2R024604 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 12:25:46 -0500 8, 25 -- Received: from [127.0.0.1] (pc2524c.dhcp.clas.ufl.edu [128.227.60.197]) 8, 25 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j6RHPg1L085094 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 13:25:44 -0400 8, 25 -- Message-ID: {42E7C396.6020408-at-ufl.edu} 8, 25 -- Date: Wed, 27 Jul 2005 13:25:42 -0400 8, 25 -- From: Greg Erdos {gwe-at-ufl.edu} 8, 25 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 8, 25 -- X-Accept-Language: en-us, en 8, 25 -- MIME-Version: 1.0 8, 25 -- To: microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] Re: user charges for SEM and related 8, 25 -- References: {200507271631.j6RGVOD7016257-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {200507271631.j6RGVOD7016257-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 7bit 8, 25 -- X-Spam-Level: ** 8, 25 -- X-UFL-Spam-Level: ** 8, 25 -- X-Spam-Status: hits=2.399, required=5, tests=BUGGY_CGI 8, 25 -- X-UFL-Spam-Status: hits=2.399, required=5, tests=BUGGY_CGI 8, 25 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 8, 25 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Hi Jon. One of the best tricks I have found over the years is to weigh out small amounts of lead citrate (0.1 to 0.4 grams) into 15 ml centrifuge tubes. When you need to make stain, add 1ml of carbonate free 1N NaOH to the tube to dissolve the lead(solution should be clear). Then add 9 mls of dd water and shake well. Put solution through a syringe filter and it is ready for use. I stain 30 seconds to 1 minute. Never have a problem with precipitate and stain is fresh every time. JoAnn At 12:27 PM 7/27/2005 -0500, you wrote:
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Department of Molecular and Cellular Physiology Stanford University School of Medicine Stanford, CA 94305 650-723-5856
==============================Original Headers============================== 6, 17 -- From redhair-at-stanford.edu Wed Jul 27 12:47:42 2005 6, 17 -- Received: from smtp3.Stanford.EDU (smtp3.Stanford.EDU [171.67.16.138]) 6, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RHlg8q004942 6, 17 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 12:47:42 -0500 6, 17 -- Received: from Bucky.stanford.edu (B135-WinXP.Stanford.EDU [171.65.21.62]) 6, 17 -- by smtp3.Stanford.EDU (8.12.11/8.12.11) with ESMTP id j6RHle9t031324 6, 17 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:47:40 -0700 6, 17 -- Message-Id: {5.1.0.14.2.20050727104209.06106790-at-redhair.pobox.stanford.edu} 6, 17 -- X-Sender: redhair-at-redhair.pobox.stanford.edu (Unverified) 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 5.1 6, 17 -- Date: Wed, 27 Jul 2005 10:47:47 -0700 6, 17 -- To: microscopy-at-microscopy.com 6, 17 -- From: JoAnn Buchanan {redhair-at-stanford.edu} 6, 17 -- Subject: Re: [Microscopy] stain solutions 6, 17 -- In-Reply-To: {200507271727.j6RHRnQ7028966-at-ns.microscopy.com} 6, 17 -- Mime-Version: 1.0 6, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Your situation is more like extension at a land grant school than work for hire.
And there are many ways to view it. If it is an ongoing thing estimated the cost of materials and labor he will use what you think it right then decide how high up the ladder you go to get a decision.
If you use any services in a similar way use them for a pattern.
For things that cost small amounts of money time it often cost less to give them away than to go the trouble of book keeping and collecting them if no method is in place to do it.
I have see it done were the party need help bought supplies for the department that helped him.
None of these are in line with accounting guide lines but use good sense as their basis. I don't know how much trouble that can get you in these days.
Gordon Gordon Couger Stillwater, OK www.couger.com/gcouger
mccaulak-at-wfu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Well, now that hopefully everyone is finished beating me down and } questioning my ethics, I'd like to offer a bit more of an explanation into } the reason for my posted question. } } First of all, my facility exists to serve my departments needs and those } needs alone. The "outsider" in question is a former student who works at } another non-profit and who is establishing a pedagogical relationship with } us which will include teaching, mentoring, and graduate student research. } This person simply asked about providing monetary compensation if the need } arose. I was at a loss for how to answer and so thought I would ask the } listserv about this. } } This is not a situation in which private labs are being undercut or a } situation in which an academic lab is pursing commercial sources of funding. } } Thanks to Debby for her instructive post on an appropriate methodology for } arriving at a cost structure. } } AM } } } } -----Original Message----- } X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU] } Sent: Wednesday, July 27, 2005 12:34 PM } To: mccaulak-at-wfu.edu } Subject: [Microscopy] Re: user charges for SEM and related } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have to say I was waiting for Chuck's response on this thread - my jaw } dropped when I read it, until the explanation came in the next e-mail!!! } } More seriously-- } } Most of the discussion on this thread has been about the harm done to } commercial labs by university labs "undercutting", "poaching customers" - } or whatever description you want to apply, and by the need to determine the } "true" cost of providing the service. I think, though, that there is a } serious problem for the university labs themselves in allowing commercial } service activities to become an important part of their operation. } } Any operation has a core mission. The proprietor of any successful } business knows the importance of identifying and maintaining their core } mission, and the ex-proprietors of many failed businesses know the costs of } straying from the mission. While individual organizations vary, the core } mission of a university characterization laboratory is not the same as that } of a commercial laboratory. In a university our basic mission is education } through the generation of research results; that of the company is to } generate a profit through providing an analytical service. These } differences, though in some areas subtle, lead to totally different } approaches to the way we interact with our clients, the way we manage our } instrumentation, our time, etc, etc. } } The government (through the NSF, in my case) contributes towards the cost } of setting up and maintaining my characterization facilities. If we } generate first-class results on new and interesting materials, then this is } considered money well-spent. If I dilute this by taking on commercial } work, even if this leads to a reduction in the amount of "subsidy", I am } reducing the rationale for getting any support, and in general diluting the } reputation of my laboratories. } } I realize that not everyone in an academic environment has my good fortune } to work for an administration that supports the ideas I have just } expressed, and I also stress again that we each work in unique } environments, but in general I would suggest that in most cases commercial } clients are best left to commercial laboratories. A commercial client is } generally looking for an analytical service, rather than to be educated. } } Tony Garratt-Reed. } } } At 10:46 PM 7/26/2005, you wrote: } } } } } } --------------------------------------------------------------------------- } } - } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 20 -- From gcc-at-couger.com Wed Jul 27 12:49:03 2005 9, 20 -- Received: from eastrmmtao01.cox.net (eastrmmtao01.cox.net [68.230.240.38]) 9, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RHn3Ev007285 9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 12:49:03 -0500 9, 20 -- Received: from [127.0.0.1] (really [68.12.43.168]) by eastrmmtao01.cox.net 9, 20 -- (InterMail vM.6.01.04.00 201-2131-118-20041027) with ESMTP 9, 20 -- id {20050727174844.NMUY12912.eastrmmtao01.cox.net-at-[127.0.0.1]} 9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 13:48:44 -0400 9, 20 -- Message-ID: {42E7C90A.1070301-at-couger.com} 9, 20 -- Date: Wed, 27 Jul 2005 12:48:58 -0500 9, 20 -- From: Gordon Couger {gcc-at-couger.com} 9, 20 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 9, 20 -- X-Accept-Language: en-us, en 9, 20 -- MIME-Version: 1.0 9, 20 -- To: microscopy-at-microscopy.com 9, 20 -- Subject: Re: [Microscopy] user charges for SEM and related 9, 20 -- References: {200507271716.j6RHGeQg018718-at-ns.microscopy.com} 9, 20 -- In-Reply-To: {200507271716.j6RHGeQg018718-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Jon, I make up my lead citrate using the Venable & Coggeshall method (0.01% Pb citrate in 10 ml of water + 1 drop 10N NaOH) and store it in a syringe that is wrapped in foil. If I keep the syringe wrapped and its tip capped, the stain is quite stable for a long time. I use a 0.2 micron filter on the syringe to dispense the stain. I usually do en bloc staining with Ur Ac in water just before I begin my dehydration steps, and so I can usually avoid staining the sections with more Ur Ac. That solution too is pretty stable if kept in a foil-wrapped bottle. I've been using a 1.5% solution, but there was a recent thread on that topic where the concensus was that newer bottles of "deplete" Ur Ac necessitated higher concentrations (up to 8%, if I remember correctly). Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
==============================Original Headers============================== 1, 23 -- From lcgould-at-med.cornell.edu Wed Jul 27 13:17:08 2005 1, 23 -- Received: from smtp-in2.med.cornell.edu (smtp-in2.med.cornell.edu [140.251.0.25]) 1, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RIH7uG020644 1, 23 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 13:17:08 -0500 1, 23 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 23 -- by smtp-in2.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6RIH2kA269492 1, 23 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 14:17:06 -0400 1, 23 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu 1, 23 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 23 -- with ESMTP id {0IKA000INTGDGM80-at-mpx1.med.cornell.edu} for 1, 23 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 14:17:01 -0400 (EDT) 1, 23 -- Date: Wed, 27 Jul 2005 14:16:58 -0400 1, 23 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 23 -- Subject: Re: [Microscopy] stain solutions 1, 23 -- In-reply-to: {200507271723.j6RHNotS023022-at-ns.microscopy.com} 1, 23 -- X-Sender: lcgould-at-pop.med.cornell.edu 1, 23 -- To: microscopy-at-microscopy.com 1, 23 -- Cc: jmkrupp-at-cats.ucsc.edu 1, 23 -- Message-id: {p06020402bf0d7e1d4916-at-[140.251.145.131]} 1, 23 -- MIME-version: 1.0 1, 23 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 23 -- References: {200507271723.j6RHNotS023022-at-ns.microscopy.com} 1, 23 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.7.27.20 ==============================End of - Headers==============================
I make up 10 ml of each of my stains and store them in syringes fitted with 0.2 micron filters, excluding air. They keep pretty well this way - weeks to months at a time. I wrap the uranyl acetate syringe with aluminum foil. I inspect for obvious precipitates before use. Expel several drops through the filter before use.
Are you coming to M&M in Honolulu?
Aloha, Tina
} I have a small lab, we do mostly basic EM type work. } } Now a days, we don't do much sectioning for the TEM. Once and a while } someone wants me to do some sectioning, but many weeks (months) may pass } between requests. } } I am trying to figure out a way to manage the post staining solutions so I } don't have to mix them up every time and/or how to make up just a little } for the occasional job. } } Any neat tricks or systems for storing post stains like lead citrate and } uranyl acetate out there? } } How about storage life time and/or ideas about mixing minimum quantities.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 8, 20 -- From tina-at-pbrc.hawaii.edu Wed Jul 27 13:24:55 2005 8, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RIOsUl028424 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 13:24:54 -0500 8, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 8, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id j6RIOpvA017338 8, 20 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 08:24:51 -1000 (HST) 8, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id j6RIOoaU017335 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 08:24:50 -1000 (HST) 8, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 8, 20 -- Date: Wed, 27 Jul 2005 08:24:49 -1000 (HST) 8, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 8, 20 -- X-Sender: tina-at-halia 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- Subject: Re: [Microscopy] stain solutions 8, 20 -- In-Reply-To: {200507271724.j6RHO7BW023802-at-ns.microscopy.com} 8, 20 -- Message-ID: {Pine.GSO.4.21.0507270818580.17267-100000-at-halia} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
I have to slightly disagree with the long time debate about academic vs commercial labs.
My scope was partially purchased with non-NSF funds. Those funds were to encourage non-academic use.
Additionally, we have an administration that strongly encourages us to help local industry with University resources.
Infact, we have commercial consultants who use our equipment.
Hence, that is not competition.
regards,
JQuinn
********************************************************** Dr. Jim Quinn james.quinn-at-stonybrook.edu Materials Science 631-632-6663 FAX:8052 Stony Brook University www.matscieng.stonybrook.edu Stony Brook, New York 11794 - 2275 **********************************************************
==============================Original Headers============================== 13, 12 -- From jquinn-at-www.matscieng.sunysb.edu Wed Jul 27 13:25:14 2005 13, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 13, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RIPD0H029252 13, 12 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 13:25:14 -0500 13, 12 -- Received: (from jquinn-at-localhost) 13, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id j6RIIkU13391 13, 12 -- for microscopy-at-microscopy.com; Wed, 27 Jul 2005 14:18:46 -0400 13, 12 -- Date: Wed, 27 Jul 2005 14:18:46 -0400 13, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 13, 12 -- Message-Id: {200507271818.j6RIIkU13391-at-www.matscieng.sunysb.edu} 13, 12 -- To: microscopy-at-microscopy.com 13, 12 -- Subject: re: user charges for SEM and related ==============================End of - Headers==============================
I'm sure everyone laughed out loud at this posting.
Thanks for brightening up our day!!!
John
} } Please accept my apologies for inadvertently sending out a message that was } going to an individual and was obviously not intended for the list at large. } } I am embarrassed about this and will try to make sure this never happens } again. } } Chuck } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.2spi.com } ######################## } ============================================ } } } } } ==============================Original Headers============================== } 7, 13 -- From cgarber-at-2spi.com Tue Jul 26 21:51:09 2005 } 7, 13 -- Received: from sccrmhc12.comcast.net (sccrmhc12.comcast.net } [204.127.202.56]) } 7, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6R2p9Eo003595 } 7, 13 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 26 Jul 2005 } 21:51:09 -0500 } 7, 13 -- Message-Id: {200507270251.j6R2p9Eo003595-at-ns.microscopy.com} } 7, 13 -- Received: from [127.0.0.1] } (pcp02988842pcs.malvrn01.pa.comcast.net[68.85.250.247]) } 7, 13 -- by comcast.net (sccrmhc12) with SMTP } 7, 13 -- id {20050727025107012009vmd7e} ; Wed, 27 Jul 2005 } 02:51:07 +0000 } 7, 13 -- To: MICROSCOPY BB {Microscopy-at-msa.microscopy.com} } 7, 13 -- Subject: Apology to the list } 7, 13 -- Date: Tue, 26 Jul 2005 22:51:18 -0500 } 7, 13 -- From: "Garber, Charles A." {cgarber-at-2spi.com} } 7, 13 -- X-Mailer: E-Mail Connection v3.1a } ==============================End of - Headers==============================
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
==============================Original Headers============================== 8, 18 -- From bozzola-at-siu.edu Wed Jul 27 13:25:28 2005 8, 18 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 8, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RIPR4j029979 8, 18 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 13:25:27 -0500 8, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 8, 18 -- by abbmta2.siu.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6RIPOxE020412 8, 18 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 13:25:25 -0500 (CDT) 8, 18 -- Mime-Version: 1.0 8, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu 8, 18 -- Message-Id: {p06110401bf0d81cf0e35-at-[131.230.177.142]} 8, 18 -- In-Reply-To: {200507270252.j6R2q5pm005634-at-ns.microscopy.com} 8, 18 -- References: {200507270252.j6R2q5pm005634-at-ns.microscopy.com} 8, 18 -- Date: Wed, 27 Jul 2005 13:25:21 -0500 8, 18 -- To: microscopy-at-microscopy.com 8, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 8, 18 -- Subject: Re: [Microscopy] Apology to the list 8, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 8, 18 -- X-MASF: 0.00% ==============================End of - Headers==============================
We are currently considering adding security cameras in each of our EM labs.
We have a very large number of users, both during working hours and after-hours in SEM and TEM rooms. Just recently, we have had a few incidents where people have damaged the instrument and did not come forward to admit it.
Although we can see who logged on to the computers, you can still damage the instrument without logging in. We believe that a CCTV in each of the labs would be the best way to solve this problem, but unfortunately we are faced with "privacy issues". People do not want to be watched.
Please tell me if your labs use cameras (live or log) or if you found other ways to get around the problem.
Thanks, Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
Is there anyone out in microscopy land that might be willing to let me borrow a Keithley picoammeter. I wold like to do some experiments with an ion gun. The unit that came with the Gatan analytical stage is what I would like to get my hands on for a few days. I believe that the model number is a 480. I am not adverse to going to my new boss with some spirited horse trading if you can help me out, especially if you might be willing to part with it permanently. If someone has an even older model that they might want to part with like a 601 or 610, that would be useful to me also.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
==============================Original Headers============================== 7, 24 -- From walck-at-southbaytech.com Wed Jul 27 13:49:54 2005 7, 24 -- Received: from ylpvm12.prodigy.net (ylpvm12-ext.prodigy.net [207.115.57.43]) 7, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RInru2027043 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 13:49:54 -0500 7, 24 -- Received: from ylpvm01.prodigy.net (ylpvm01-int.prodigy.net [207.115.5.207]) 7, 24 -- by ylpvm12.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id j6RInjck020579 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 14:49:45 -0400 7, 24 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 7, 24 -- by ylpvm01.prodigy.net (8.13.4 dk-milter linux/8.13.4) with ESMTP id j6RInoE0031623 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 14:49:50 -0400 7, 24 -- From: "Scott Walck" {walck-at-southbaytech.com} 7, 24 -- To: {Microscopy-at-microscopy.com} 7, 24 -- Subject: picoammeter availability 7, 24 -- Date: Wed, 27 Jul 2005 11:50:17 -0700 7, 24 -- Message-ID: {000701c592dc$08525100$7801a8c0-at-dynamicbl8uno3} 7, 24 -- MIME-Version: 1.0 7, 24 -- Content-Type: text/plain; 7, 24 -- charset="US-ASCII" 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- X-Priority: 3 (Normal) 7, 24 -- X-MSMail-Priority: Normal 7, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 7, 24 -- Importance: Normal 7, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Use either Netscape or IE browsers , Safari access is currently broken I'll fix that after the MM2005 meeting.
Nestor Your Friendly Neighborhood SysOp
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
How on earth do you read Nestor's email as "derogatory and demeaning"?
Do you see some equivalence between saying "I don't care who you are" and describing someone else as practising "crap journalism"?
What color is the sky in your world?
And how about having the courage to identify yourself?
Anonymous postings don't rate very highly with most people.
cheers
rtch
Date sent: Wed, 27 Jul 2005 09:42:24 -0500 To: r.sims-at-auckland.ac.nz X-from: bmollon-at-pacbell.net Send reply to: microscopy-at-microscopy.com
Hey guys, I thing some of this is coming down to the issue of reading tone/intent into words in print. When Nestor wrote "I don't care who you are" , MY interpretation was "no one has special rights or privileges" not "I don't give a damn about you". (Just my humble reading). Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
==============================Original Headers============================== 1, 22 -- From lcgould-at-med.cornell.edu Wed Jul 27 15:06:29 2005 1, 22 -- Received: from smtp-in2.med.cornell.edu (smtp-in2.med.cornell.edu [140.251.0.25]) 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RK6SGq018967 1, 22 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 15:06:29 -0500 1, 22 -- Received: from mpx2.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 22 -- by smtp-in2.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6RK6RFU136116 1, 22 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 16:06:27 -0400 1, 22 -- Received: from [140.251.145.131] by mpx2.med.cornell.edu 1, 22 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 22 -- with ESMTP id {0IKA0063BYIQFDA0-at-mpx2.med.cornell.edu} for 1, 22 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 16:06:26 -0400 (EDT) 1, 22 -- Date: Wed, 27 Jul 2005 16:06:23 -0400 1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 22 -- Subject: [Microscopy] Re: rules 1, 22 -- In-reply-to: {200507271928.j6RJS3aE011900-at-ns.microscopy.com} 1, 22 -- X-Sender: lcgould-at-pop.med.cornell.edu 1, 22 -- To: microscopy-at-microscopy.com 1, 22 -- Message-id: {p06020405bf0d98be8a9e-at-[140.251.145.131]} 1, 22 -- MIME-version: 1.0 1, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 22 -- References: {200507271928.j6RJS3aE011900-at-ns.microscopy.com} 1, 22 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.7.27.24 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Wednesday, July 27, 2005 at 10:27:20 ---------------------------------------------------------------------------
Email: K.venner-at-ion.ucl.ac.uk Name: Kerrie
Organization: ION, UCL, UK
Title-Subject: [Filtered] tem flat embedding moulds
Question: I have some well used, much coveted flat embedding moulds for TEM samples. They are shallow, hexagonal-shaped recesses with an arrowhead on one side, and are in a pale blue rubbery material. Unfortunately they are beginning to perish, and we have no idea where we bought them from originally. We are desperate to replace them. Any ideas or suppliers out there?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (deerinck-at-ncmir.ucsd.edu) from http://microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, July 27, 2005 at 12:48:11 ---------------------------------------------------------------------------
Email: deerinck-at-ncmir.ucsd.edu Name: Tom Deerinck
Organization: NCMIR, UCSD
Title-Subject: [Filtered] MListserver:
Question: Staff Positions Available at the National Center for Microscopy and Imaging Research at the University of California, San Diego, CA
Two staff positions in TEM and 3D tomographic reconstruction are available immediately. The successful candidate should have a BS or MS in relevant fields of biological sciences and have experience in either 1) transmission electron microscopy or 2) image processing and/or three-dimensional reconstruction techniques.
Qualifications for the first position (Transmission Electron Microscopist) are: _ Experienced TEM operation (operation, alignment, image recording) _ Proficiency in sectioning of plastic embedded biological specimens (microtomy) _ Familiarity in preparation of biological material for plastic embedding (fixation, dehydration and embedding techniques) _ Experience in bacteriology, immunocytochemistry, cell biology or fluorescence/confocal microscopy preferred but not essential.
Qualifications for the second position (Image Processing Scientist) are: _ Experience in image processing and evaluating EM images _ Experience in three-dimensional tomographic reconstruction _ Familiarity using Linux or Unix, Mac and PC operating systems _ Proficiency in computer visualization and/or animation package _ General biology background with a preferred emphasis on bacterial systems.
Excellent written and verbal skills are expected. The candidates will be working in a large interdisciplinary research center located in the University of California San Diego main campus and will have access to state of the art electron microscopes. For more information on our laboratory, please visit http://www.ncmir.ucsd.edu. Please forward this message to all appropriate personnel. Applicants should send curriculum vitae, bibliography, a brief description of present research activities and plans and the names and contact information of three references to:
Dr. Mark Ellisman or Mr. Thomas Deerinck University of California at San Diego 1000 Basic Science Building MC 0608 9500 Gilman Drive La Jolla, CA 92093-0608 858-534-4583 (phone) mark-at-ncmir.ucsd.edu (email) 858-534-7497 (fax) deerinck-at-ncmir.ucsd.edu
We will also be available at the MSA meeting next week in Hawaii.
They sound like Chien molds to me....I know that EMS carries them, probably other suppliers too. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
==============================Original Headers============================== 1, 23 -- From lcgould-at-med.cornell.edu Wed Jul 27 15:25:38 2005 1, 23 -- Received: from smtp-in2.med.cornell.edu (smtp-in2.med.cornell.edu [140.251.0.25]) 1, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RKPXtk010161 1, 23 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 15:25:33 -0500 1, 23 -- Received: from mpx2.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 23 -- by smtp-in2.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j6RKPUQU231430 1, 23 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 16:25:30 -0400 1, 23 -- Received: from [140.251.145.131] by mpx2.med.cornell.edu 1, 23 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 23 -- with ESMTP id {0IKA00AKZZEH1B00-at-mpx2.med.cornell.edu} for 1, 23 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 16:25:30 -0400 (EDT) 1, 23 -- Date: Wed, 27 Jul 2005 16:25:26 -0400 1, 23 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 23 -- Subject: Re: [Microscopy] viaWWW: tem flat embedding moulds 1, 23 -- In-reply-to: {200507272012.j6RKCJ9H025298-at-ns.microscopy.com} 1, 23 -- X-Sender: lcgould-at-pop.med.cornell.edu 1, 23 -- To: microscopy-at-microscopy.com 1, 23 -- Cc: K.venner-at-ion.ucl.ac.uk 1, 23 -- Message-id: {p06020407bf0d9decc23d-at-[140.251.145.131]} 1, 23 -- MIME-version: 1.0 1, 23 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 23 -- References: {200507272012.j6RKCJ9H025298-at-ns.microscopy.com} 1, 23 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.7.27.24 ==============================End of - Headers==============================
} From: Winston.Wiggins-at-cshs.org } Reply-To: microscopy-at-microscopy.com } Date: Wed, 27 Jul 2005 10:54:20 -0500 } To: leswes-at-shaw.ca } Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } List members et al., } Is there any way we can "restore" the system to the point before the I.Q. } posting, thereby returning Sergey to the List? I would hate to miss his } often insightful comments. I also hate to think that any comment, understood } or misunderstood, made in the heat of passion may summarily cause expulsion } sans appeal, deservedly or not. } "Can we all just get along?!" } Winston } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Winston W. Wiggins, E.M. Pathology Asst. } CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine } Electron Microscopy, LLSPT, A823-A828 } 8700 Beverly Blvd. } Los Angeles, CA 90048 } 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax) } Winston.Wiggins-at-CSHS.org } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } -----Original Message----- } X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] } Sent: Tuesday, July 26, 2005 5:12 PM } To: Winston.Wiggins-at-CSHS.org } Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Person } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Sergey } } A honest discussion or even disagreement about a subject or work which is } microscopy } related (even tangentially) is not prohibited. } } However, it is against the rules to intentionally disparage any person } or organization on the Listserver. This rule applies equally to } Journalists as well } as Microscopists. Please review the rules which you received upon } subscription. } } } http://microscopy.com/MicroscopyListserver/Rules.html } } Presumably a person of hi IQ has the ability to distinguish between these } two. } } Nestor } Your Friendly Neighborhood SysOp } } } --------------------------------------------------------------------------- } - } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------------- } - } } } } Nestor } } I don't see any reason why we could not discuss the quality of somebody's } } work even if it's a journalist with former (?) high IQ. Is it prohibited } } to discuss people's work with high IQ on this ListServer? Sergey } } } } At 03:51 PM 7/26/2005, you wrote: } } --------------------------------------------------------------------------- } - } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------------------- } -- } } } } } } Beth & everyone else } } } } } } There was no problem with Beth's initial posting and no apology } } } was needed here Beth. Sorry if I appeared to have come down on you. } } } } } } The followups IMHO were starting to drift into critiques of Marilyn } whomever } } } and her "crap Journalism" and I was trying to nip that direction of } } } critique in the bud. However, I see I had the opposite effect. Oh } well..... } } } } } } As Dorrance said, everyone can use a grin occassionally . } } } } } } } } } Nestor } } } } } } } } } -------------------------------------------------------------------------- } -- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } --------------------------------------------------------------------------- } - } } } } } } } } I apologize to the list for posting Marilyn vos Savant's statement on } } } } microscopy. I just wanted to share the article because I thought it was } } } } humorous...I shall refrain from such postings. } } } } best, } } } } Beth } } } } } } } } ********************************************************************** } } } } Beth Richardson } } } } EM Lab Coordinator } } } } Plant Biology Department } } } } University of Georgia } } } } Athens, GA 30602-7271 } } } } } } } } Phone - (706) 542-1790 & FAX - (706) 542-1805 } } } } http://www.plantbio.uga.edu/emlab } } } } } } } } "Between the two evils, } } } } I always pick the one I never tried before". Mae West (1893-1980) } } } } ******************************************************************* } } } } } } } } "And it's only the giving that makes you what you are". } } } } Wond'ring Aloud, Jethro Tull (Aqualung) } } } } } } } } ************************************************************************ } } } } } Important Warning: This message is intended for the use of the person or } entity to which it is addressed and may contain information that is } privileged and confidential, the disclosure of which is governed by } aplicable law. If the reader is not the intended recipient, or the employee } or agent responsible for delivering it to the intended recipient, you are } hereby notified that any dissemination, distribution or copying of this } information is STRICTLY PROHIBITED. } } If you have received this message in error, please notify us immediately, } by calling (310) 423-6428 -- and destroy the related message. Thank You for } your cooperation. } } } } ==============================Original Headers============================== } 18, 25 -- From winston.wiggins-at-cshs.org Wed Jul 27 10:46:49 2005 } 18, 25 -- Received: from csip1.csmc.edu (CSIP1.csmc.edu [192.231.133.35]) } 18, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RFkmjF031434 } 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:46:48 -0500 } 18, 25 -- Received: from unknown (HELO zixvpm01.csmc.edu) (192.168.10.26) } 18, 25 -- by csip1.csmc.edu with ESMTP; 27 Jul 2005 08:46:48 -0700 } 18, 25 -- X-BrightmailFiltered: true } 18, 25 -- X-Brightmail-Tracker: AAAAAA== } 18, 25 -- Received: from zixvpm01.csmc.edu (ZixVPM [127.0.0.1]) } 18, 25 -- by Outbound.cshs.org (Proprietary) with ESMTP id 2BF4A4BDC8 } 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:03:55 -0500 } (CDT) } 18, 25 -- Received: from exchange1.csmc.edu (SMTP.csmc.edu [166.124.43.199]) } 18, 25 -- by zixvpm01.csmc.edu (Proprietary) with ESMTP id 0132AA7B6F } 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:03:55 -0500 } (CDT) } 18, 25 -- Received: by SMTP.csmc.edu with Internet Mail Service (5.5.2653.19) } 18, 25 -- id {PXCNG821} ; Wed, 27 Jul 2005 08:46:47 -0700 } 18, 25 -- Message-ID: } {B5AEAEDBB5D44C47BFE2A6D791329D7F2ADC7D-at-EXCHANGE24.csmc.edu} } 18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} } 18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } 18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of } a Pers } 18, 25 -- on } 18, 25 -- Date: Wed, 27 Jul 2005 08:46:46 -0700 } 18, 25 -- MIME-Version: 1.0 } 18, 25 -- X-Mailer: Internet Mail Service (5.5.2653.19) } 18, 25 -- Content-Type: text/plain } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 27 -- From leswes-at-shaw.ca Wed Jul 27 15:57:28 2005 5, 27 -- Received: from pd4mo2so.prod.shaw.ca (shawidc-mo1.cg.shawcable.net [24.71.223.10]) 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RKvRhp018496 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 15:57:27 -0500 5, 27 -- Received: from pd3mr3so.prod.shaw.ca 5, 27 -- (pd3mr3so-qfe3.prod.shaw.ca [10.0.141.179]) by l-daemon 5, 27 -- (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 5, 27 -- with ESMTP id {0IKB00BLI0VQHB00-at-l-daemon} for microscopy-at-microscopy.com; Wed, 5, 27 -- 27 Jul 2005 14:57:26 -0600 (MDT) 5, 27 -- Received: from pn2ml9so.prod.shaw.ca ([10.0.121.7]) 5, 27 -- by pd3mr3so.prod.shaw.ca (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 5, 27 -- 15 2004)) with ESMTP id {0IKB00IKB0VQI2O0-at-pd3mr3so.prod.shaw.ca} for 5, 27 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 14:57:26 -0600 (MDT) 5, 27 -- Received: from [24.85.86.83] (S0106000a27de81ae.vc.shawcable.net [24.85.86.83]) 5, 27 -- by l-daemon (iPlanet Messaging Server 5.2 HotFix 1.18 (built Jul 28 2003)) 5, 27 -- with ESMTP id {0IKB0001S0VQI2-at-l-daemon} for microscopy-at-microscopy.com; Wed, 5, 27 -- 27 Jul 2005 14:57:26 -0600 (MDT) 5, 27 -- Date: Wed, 27 Jul 2005 13:58:42 -0700 5, 27 -- From: Lesley Weston {leswes-at-shaw.ca} 5, 27 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers 5, 27 -- In-reply-to: {200507271554.j6RFsKKs004617-at-ns.microscopy.com} 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- Message-id: {BF0D4392.1FA5%leswes-at-shaw.ca} 5, 27 -- MIME-version: 1.0 5, 27 -- Content-type: text/plain; charset=US-ASCII 5, 27 -- Content-transfer-encoding: 7bit 5, 27 -- User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.0.6 ==============================End of - Headers==============================
-----Original Message----- X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca] Sent: Wednesday, July 27, 2005 3:58 PM To: Foran, David A
I'll second this.
-- Lesley Weston
} From: Winston.Wiggins-at-cshs.org } Reply-To: microscopy-at-microscopy.com } Date: Wed, 27 Jul 2005 10:54:20 -0500 } To: leswes-at-shaw.ca } Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a } Pers } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } List members et al., } Is there any way we can "restore" the system to the point before the } I.Q. posting, thereby returning Sergey to the List? I would hate to } miss his often insightful comments. I also hate to think that any } comment, understood or misunderstood, made in the heat of passion may } summarily cause expulsion sans appeal, deservedly or not. "Can we all } just get along?!" Winston } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Winston W. Wiggins, E.M. Pathology Asst. } CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron } Microscopy, LLSPT, A823-A828 8700 Beverly Blvd. } Los Angeles, CA 90048 } 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax) } Winston.Wiggins-at-CSHS.org } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } -----Original Message----- } X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] } Sent: Tuesday, July 26, 2005 5:12 PM } To: Winston.Wiggins-at-CSHS.org } Subject: [Microscopy] Discussion of high IQ vs Disparagement of a } Person } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Sergey } } A honest discussion or even disagreement about a subject or work } which is microscopy related (even tangentially) is not prohibited. } } However, it is against the rules to intentionally disparage any person } or organization on the Listserver. This rule applies equally to } Journalists as well as Microscopists. Please review the rules which } you received upon } subscription. } } } http://microscopy.com/MicroscopyListserver/Rules.html } } Presumably a person of hi IQ has the ability to distinguish between } these two. } } Nestor } Your Friendly Neighborhood SysOp } } } --------------------------------------------------------------------- } } ------ } - } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------------- } - } } } } Nestor } } I don't see any reason why we could not discuss the quality of } } somebody's work even if it's a journalist with former (?) high IQ. } } Is it prohibited to discuss people's work with high IQ on this } } ListServer? Sergey } } } } At 03:51 PM 7/26/2005, you wrote: } } --------------------------------------------------------------------- } } ------ } - } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------------- } } } ------ } -- } } } } } } Beth & everyone else } } } } } } There was no problem with Beth's initial posting and no apology } } } was needed here Beth. Sorry if I appeared to have come down on you. } } } } } } The followups IMHO were starting to drift into critiques of } } } Marilyn } whomever } } } and her "crap Journalism" and I was trying to nip that direction of } } } critique in the bud. However, I see I had the opposite effect. Oh } well..... } } } } } } As Dorrance said, everyone can use a grin occassionally . } } } } } } } } } Nestor } } } } } } } } } -------------------------------------------------------------------- } } } ------ } -- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help } } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } --------------------------------------------------------------------- } } ------ } - } } } } } } } } I apologize to the list for posting Marilyn vos Savant's statement } } } } on microscopy. I just wanted to share the article because I thought } } } } it was humorous...I shall refrain from such postings. best, } } } } Beth } } } } } } } } ******************************************************************* } } } } *** } } } } Beth Richardson } } } } EM Lab Coordinator } } } } Plant Biology Department } } } } University of Georgia } } } } Athens, GA 30602-7271 } } } } } } } } Phone - (706) 542-1790 & FAX - (706) 542-1805 } } } } http://www.plantbio.uga.edu/emlab } } } } } } } } "Between the two evils, } } } } I always pick the one I never tried before". Mae West (1893-1980) } } } } ******************************************************************* } } } } } } } } "And it's only the giving that makes you what you are". Wond'ring } } } } Aloud, Jethro Tull (Aqualung) } } } } } } } } ******************************************************************* } } } } ***** } } } } } Important Warning: This message is intended for the use of the person } or entity to which it is addressed and may contain information that is } privileged and confidential, the disclosure of which is governed by } aplicable law. If the reader is not the intended recipient, or the } employee or agent responsible for delivering it to the intended } recipient, you are hereby notified that any dissemination, } distribution or copying of this information is STRICTLY PROHIBITED. } } If you have received this message in error, please notify us } immediately, by calling (310) 423-6428 -- and destroy the related } message. Thank You for your cooperation. } } } } ==============================Original } Headers============================== } 18, 25 -- From winston.wiggins-at-cshs.org Wed Jul 27 10:46:49 2005 } 18, 25 -- Received: from csip1.csmc.edu (CSIP1.csmc.edu [192.231.133.35]) } 18, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RFkmjF031434 } 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:46:48 -0500 } 18, 25 -- Received: from unknown (HELO zixvpm01.csmc.edu) (192.168.10.26) } 18, 25 -- by csip1.csmc.edu with ESMTP; 27 Jul 2005 08:46:48 -0700 } 18, 25 -- X-BrightmailFiltered: true } 18, 25 -- X-Brightmail-Tracker: AAAAAA== } 18, 25 -- Received: from zixvpm01.csmc.edu (ZixVPM [127.0.0.1]) } 18, 25 -- by Outbound.cshs.org (Proprietary) with ESMTP id 2BF4A4BDC8 } 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:03:55 -0500 } (CDT) } 18, 25 -- Received: from exchange1.csmc.edu (SMTP.csmc.edu [166.124.43.199]) } 18, 25 -- by zixvpm01.csmc.edu (Proprietary) with ESMTP id 0132AA7B6F } 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:03:55 -0500 } (CDT) } 18, 25 -- Received: by SMTP.csmc.edu with Internet Mail Service (5.5.2653.19) } 18, 25 -- id {PXCNG821} ; Wed, 27 Jul 2005 08:46:47 -0700 } 18, 25 -- Message-ID: } {B5AEAEDBB5D44C47BFE2A6D791329D7F2ADC7D-at-EXCHANGE24.csmc.edu} } 18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} } 18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } 18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of } a Pers } 18, 25 -- on } 18, 25 -- Date: Wed, 27 Jul 2005 08:46:46 -0700 } 18, 25 -- MIME-Version: 1.0 } 18, 25 -- X-Mailer: Internet Mail Service (5.5.2653.19) } 18, 25 -- Content-Type: text/plain } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 27 -- From leswes-at-shaw.ca Wed Jul 27 15:57:28 2005 5, 27 -- Received: from pd4mo2so.prod.shaw.ca (shawidc-mo1.cg.shawcable.net [24.71.223.10]) 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RKvRhp018496 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 15:57:27 -0500 5, 27 -- Received: from pd3mr3so.prod.shaw.ca 5, 27 -- (pd3mr3so-qfe3.prod.shaw.ca [10.0.141.179]) by l-daemon 5, 27 -- (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 5, 27 -- with ESMTP id {0IKB00BLI0VQHB00-at-l-daemon} for microscopy-at-microscopy.com; Wed, 5, 27 -- 27 Jul 2005 14:57:26 -0600 (MDT) 5, 27 -- Received: from pn2ml9so.prod.shaw.ca ([10.0.121.7]) 5, 27 -- by pd3mr3so.prod.shaw.ca (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 5, 27 -- 15 2004)) with ESMTP id {0IKB00IKB0VQI2O0-at-pd3mr3so.prod.shaw.ca} for 5, 27 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 14:57:26 -0600 (MDT) 5, 27 -- Received: from [24.85.86.83] (S0106000a27de81ae.vc.shawcable.net [24.85.86.83]) 5, 27 -- by l-daemon (iPlanet Messaging Server 5.2 HotFix 1.18 (built Jul 28 2003)) 5, 27 -- with ESMTP id {0IKB0001S0VQI2-at-l-daemon} for microscopy-at-microscopy.com; Wed, 5, 27 -- 27 Jul 2005 14:57:26 -0600 (MDT) 5, 27 -- Date: Wed, 27 Jul 2005 13:58:42 -0700 5, 27 -- From: Lesley Weston {leswes-at-shaw.ca} 5, 27 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers 5, 27 -- In-reply-to: {200507271554.j6RFsKKs004617-at-ns.microscopy.com} 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- Message-id: {BF0D4392.1FA5%leswes-at-shaw.ca} 5, 27 -- MIME-version: 1.0 5, 27 -- Content-type: text/plain; charset=US-ASCII 5, 27 -- Content-transfer-encoding: 7bit 5, 27 -- User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.0.6 ==============================End of - Headers==============================
==============================Original Headers============================== 12, 17 -- From DFORAN-at-ORA.FDA.GOV Wed Jul 27 16:00:11 2005 12, 17 -- Received: from walltiger-pub.fda.gov (walltiger-pub.fda.gov [150.148.0.28]) 12, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RL0A6p022045 12, 17 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 16:00:11 -0500 12, 17 -- Received: from orshq08a.fda.gov by walltiger-pub.fda.gov 12, 17 -- via smtpd (for microscopy.com [206.69.208.10]) with ESMTP; Wed, 27 Jul 2005 17:00:11 -0400 12, 17 -- Received: by orshq08a.fda.gov with Internet Mail Service (5.5.2657.72) 12, 17 -- id {NFL92QN2} ; Wed, 27 Jul 2005 17:00:11 -0400 12, 17 -- Message-ID: {D117D2B78C6BA24C9500F2431F6F260A01F6F908-at-orsswkc02.fda.gov} 12, 17 -- From: "Foran, David A" {DFORAN-at-ORA.FDA.GOV} 12, 17 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 12, 17 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of a Per 12, 17 -- s 12, 17 -- Date: Wed, 27 Jul 2005 17:00:09 -0400 12, 17 -- MIME-Version: 1.0 12, 17 -- X-Mailer: Internet Mail Service (5.5.2657.72) 12, 17 -- Content-Type: text/plain ==============================End of - Headers==============================
On 7/27/05 2:00 PM, "DFORAN-at-ORA.FDA.GOV" {DFORAN-at-ORA.FDA.GOV} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Aye. } } -----Original Message----- } X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca] } Sent: Wednesday, July 27, 2005 3:58 PM } To: Foran, David A } Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'll second this.
==============================Original Headers============================== 5, 18 -- From tiekotte-at-up.edu Wed Jul 27 16:02:23 2005 5, 18 -- Received: from london.campus.up.edu (london.campus.up.edu [64.251.248.18]) 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RL2N0o026609 5, 18 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 16:02:23 -0500 5, 18 -- Received: from 165.121.33.173 ([165.121.33.173]) by london.campus.up.edu ([10.5.128.61]) via Exchange Front-End Server webmail.up.edu ([64.251.254.43]) with Microsoft Exchange Server HTTP-DAV ; 5, 18 -- Wed, 27 Jul 2005 21:02:22 +0000 5, 18 -- User-Agent: Microsoft-Entourage/11.0.0.040405 5, 18 -- Date: Wed, 27 Jul 2005 14:02:21 -0700 5, 18 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs Disparagement of a 5, 18 -- Per 5, 18 -- From: Ken Tiekotter {tiekotte-at-up.edu} 5, 18 -- To: {microscopy-at-microscopy.com} 5, 18 -- Message-ID: {BF0D446D.239B%tiekotte-at-up.edu} 5, 18 -- In-Reply-To: {200507272100.j6RL0QNc022921-at-ns.microscopy.com} 5, 18 -- Mime-version: 1.0 5, 18 -- Content-type: text/plain; 5, 18 -- charset="US-ASCII" 5, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
They are Chien embedding molds. We got ours from Ted Pella. Tom
Tom Januszewski Senior Electron Microscopist Molecular and Cellular Imaging Facility UT Southwestern Medical Center at Dallas Dallas, TX 75390-9039 214-648-7291 FAX 214-648-6408 tom.januszewski-at-UTSouthwestern.edu
==============================Original Headers============================== 3, 17 -- From Tom.Januszewski-at-UTSouthwestern.edu Wed Jul 27 16:14:16 2005 3, 17 -- Received: from mednet.swmed.edu (swnw124.swmed.edu [199.165.152.124]) 3, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RLEGuI009757 3, 17 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 16:14:16 -0500 3, 17 -- Received: from SWSMTP-MTA by mednet.swmed.edu 3, 17 -- with Novell_GroupWise; Wed, 27 Jul 2005 16:12:51 -0500 3, 17 -- Message-Id: {s2e7b283.039-at-mednet.swmed.edu} 3, 17 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 3, 17 -- Date: Wed, 27 Jul 2005 16:12:34 -0500 3, 17 -- From: "Tom Januszewski" {Tom.Januszewski-at-UTSouthwestern.edu} 3, 17 -- To: {microscopy-at-microscopy.com} 3, 17 -- Subject: Re: [Microscopy] viaWWW: tem flat embedding moulds 3, 17 -- Mime-Version: 1.0 3, 17 -- Content-Type: text/plain; charset=US-ASCII 3, 17 -- Content-Disposition: inline 3, 17 -- Content-Transfer-Encoding: 8bit 3, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6RLEGuI009757 ==============================End of - Headers==============================
} -----Original Message----- } From: DFORAN-at-ORA.FDA.GOV [mailto:DFORAN-at-ORA.FDA.GOV] } Sent: Wednesday, July 27, 2005 4:01 PM } To: Dusevich, Vladimir } Subject: [Microscopy] RE: Discussion of high IQ vs } Disparagement of a Per } } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } Aye. } } -----Original Message----- } X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca] } Sent: Wednesday, July 27, 2005 3:58 PM } To: Foran, David A } Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers } } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } I'll second this. } } -- } Lesley Weston } } } } From: Winston.Wiggins-at-cshs.org } } Reply-To: microscopy-at-microscopy.com } } Date: Wed, 27 Jul 2005 10:54:20 -0500 } } To: leswes-at-shaw.ca } } Subject: [Microscopy] RE: Discussion of high IQ vs } Disparagement of a } } Pers } } } } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------- } -------------- } } } } List members et al., } } Is there any way we can "restore" the system to the point before the } } I.Q. posting, thereby returning Sergey to the List? I would hate to } } miss his often insightful comments. I also hate to think that any } } comment, understood or misunderstood, made in the heat of } passion may } } summarily cause expulsion sans appeal, deservedly or not. } "Can we all } } just get along?!" Winston } } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } Winston W. Wiggins, E.M. Pathology Asst. } } CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron } } Microscopy, LLSPT, A823-A828 8700 Beverly Blvd. } } Los Angeles, CA 90048 } } 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax) } } Winston.Wiggins-at-CSHS.org } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } } } -----Original Message----- } } X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] } } Sent: Tuesday, July 26, 2005 5:12 PM } } To: Winston.Wiggins-at-CSHS.org } } Subject: [Microscopy] Discussion of high IQ vs Disparagement of a } } Person } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------- } -------------- } } } } Sergey } } } } A honest discussion or even disagreement about a subject or work } } which is microscopy related (even tangentially) is not prohibited. } } } } However, it is against the rules to intentionally disparage } any person } } or organization on the Listserver. This rule applies equally to } } Journalists as well as Microscopists. Please review the } rules which } } you received upon } } subscription. } } } } } } http://microscopy.com/MicroscopyListserver/Rules.html } } } } Presumably a person of hi IQ has the ability to distinguish between } } these two. } } } } Nestor } } Your Friendly Neighborhood SysOp } } } } } } --------------------------------------------------------------------- } } } ------ } } - } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } -------------------------------------------------------------- } ------------- } } - } } } } } } Nestor } } } I don't see any reason why we could not discuss the quality of } } } somebody's work even if it's a journalist with former (?) } high IQ. } } } Is it prohibited to discuss people's work with high IQ on this } } } ListServer? Sergey } } } } } } At 03:51 PM 7/26/2005, you wrote: } } } } --------------------------------------------------------------------- } } } ------ } } - } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } -------------------------------------------------------------------- } } } } ------ } } -- } } } } } } } } Beth & everyone else } } } } } } } } There was no problem with Beth's initial posting and no apology } } } } was needed here Beth. Sorry if I appeared to have come } down on you. } } } } } } } } The followups IMHO were starting to drift into critiques of } } } } Marilyn } } whomever } } } } and her "crap Journalism" and I was trying to nip that } direction of } } } } critique in the bud. However, I see I had the opposite effect. Oh } } well..... } } } } } } } } As Dorrance said, everyone can use a grin occassionally . } } } } } } } } } } } } Nestor } } } } } } } } } } } } } -------------------------------------------------------------------- } } } } ------ } } -- } } } } } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of } } America } } } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } } } On-Line Help
==============================Original Headers============================== 7, 23 -- From DusevichV-at-umkc.edu Wed Jul 27 16:25:16 2005 7, 23 -- Received: from kc-msxproto3.kc.umkc.edu (e2k.exchange.umkc.edu [134.193.44.10]) 7, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RLPGb3017684 7, 23 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 16:25:16 -0500 7, 23 -- Received: from KC-MAIL1.kc.umkc.edu ([134.193.32.1]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Wed, 27 Jul 2005 16:25:16 -0500 7, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5.7226.0 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: RE: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Per 7, 23 -- Date: Wed, 27 Jul 2005 16:25:15 -0500 7, 23 -- Message-ID: {4BF01C49DF1C4F4E883C3E25C670874933FF2D-at-KC-MAIL1.kc.umkc.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Per 7, 23 -- Thread-Index: AcWS7kOW97N42mZJTdCaYbStexeKCAAA2Fdw 7, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 27 Jul 2005 21:25:16.0109 (UTC) FILETIME=[AE6D27D0:01C592F1] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6RLPGb3017684 ==============================End of - Headers==============================
Tony Garratt-Reed wrote: ======================================================== I have to say I was waiting for Chuck's response on this thread - my jaw dropped when I read it, until the explanation came in the next e-mail!!! ======================================================== Perhaps I became a bit "gun shy" after sending off a message in error. I appreciate the understanding of those on the list for someone, like myself, who did make an error. It has taken me more than a few hours to recover from that.
I have operated an independent testing and analytical laboratory with its core capabilities being built around SEM/EDS and TEM and LM since 1970. I think I have at least some credentials to speak to the issue at hand. However, what I have to say might be seen as "too much" for a normal listserver posting, therefore I have posted my "reply" to URL http://www.2spi.com/letter.html
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 7, 13 -- From cgarber-at-2spi.com Wed Jul 27 17:12:36 2005 7, 13 -- Received: from sccrmhc12.comcast.net (sccrmhc12.comcast.net [204.127.202.56]) 7, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RMCZHk026498 7, 13 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 27 Jul 2005 17:12:35 -0500 7, 13 -- Message-Id: {200507272212.j6RMCZHk026498-at-ns.microscopy.com} 7, 13 -- Received: from [127.0.0.1] (pcp02988842pcs.malvrn01.pa.comcast.net[68.85.250.247]) 7, 13 -- by comcast.net (sccrmhc12) with SMTP 7, 13 -- id {2005072722123401200a4381e} ; Wed, 27 Jul 2005 22:12:34 +0000 7, 13 -- To: MICROSCOPY BB {Microscopy-at-msa.microscopy.com} 7, 13 -- Subject: University facilities being used for outside users 7, 13 -- Date: Wed, 27 Jul 2005 18:12:49 -0500 7, 13 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 7, 13 -- X-Mailer: E-Mail Connection v3.1a ==============================End of - Headers==============================
We used to showoff our instrumentation across the web through our Nanoworld site, we have 14 EM columns and most were available to view 24/7. This was until we had a nasty stalking incident that left both the client involved and our staff members unsettled until we took all cameras off air. We now do individual presentation to schools and groups as required and everyone is aware the cameras are ON.
Regards JVN
Daniel.Salamon-at-nrc-cnrc.gc.ca wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- John V Nailon Executive Officer and Operations Manager Centre for Microscopy and Microanalysis The University of Queensland St.Lucia QLD 4072 Australia Phone: 617 3365 4214 Fax: 617 3365 4422 Mobile: 0423 020 680
==============================Original Headers============================== 11, 23 -- From J.Nailon-at-uq.edu.au Wed Jul 27 17:44:00 2005 11, 23 -- Received: from mailhub1.uq.edu.au (mailhub1.uq.edu.au [130.102.148.128]) 11, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RMhxrG002315 11, 23 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 17:44:00 -0500 11, 23 -- Received: from smtp2.uq.edu.au (newsmtp2.uq.edu.au [130.102.149.129]) 11, 23 -- by mailhub1.uq.edu.au (8.13.3/8.13.3) with ESMTP id j6RMhwKo004181 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 08:43:58 +1000 (EST) 11, 23 -- Received: from uq.edu.au (budgie.cmm.uq.edu.au [152.98.195.29]) 11, 23 -- by smtp2.uq.edu.au (8.13.3/8.13.3) with ESMTP id j6RMhwkd068184 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 08:43:58 +1000 (EST) 11, 23 -- Message-ID: {42E80E2E.7050408-at-uq.edu.au} 11, 23 -- Date: Thu, 28 Jul 2005 08:43:58 +1000 11, 23 -- From: John V Nailon {J.Nailon-at-uq.edu.au} 11, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 11, 23 -- X-Accept-Language: en-us, en 11, 23 -- MIME-Version: 1.0 11, 23 -- To: microscopy-at-microscopy.com 11, 23 -- Subject: Re: [Microscopy] cameras in labs 11, 23 -- References: {200507271828.j6RISns6008698-at-ns.microscopy.com} 11, 23 -- In-Reply-To: {200507271828.j6RISns6008698-at-ns.microscopy.com} 11, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- X-Scanned-By: MIMEDefang 2.51 on UQ Mailhub on 130.102.148.128 ==============================End of - Headers==============================
I am again looking for prepared and interesting specimens for SEM/STEM or just TEM negs. Human pathogens are especially welcome. Standard 12mm pin stubs for SEM are perfect. Bacteria, parasites, cancers, etc. are good. CPD/fixed specimens coated or un-coated are fine.
Pls contact me off-line for my payment schedule and what you may have to sell (I keep it) or rent (in case you want it back).
See you in HI.
gary g.
==============================Original Headers============================== 5, 16 -- From gary-at-gaugler.com Wed Jul 27 18:19:32 2005 5, 16 -- Received: from smtp2.mc.surewest.net (smtp2.mc.surewest.net [66.60.130.51]) 5, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6RNJVH2010388 5, 16 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 18:19:32 -0500 5, 16 -- Received: (s3-27399); Wed, 27 Jul 2005 16:19:31 -0700 5, 16 -- Received: from unknown (66.60.171.211) 5, 16 -- by smtp2.mc.surewest.net (s3-smtpd/0.90-beta3) with SMTP; Wed, 27 Jul 2005 16:19:29 -0700 5, 16 -- Message-Id: {6.2.0.14.2.20050727161255.029a5908-at-mail.calweb.com} 5, 16 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 5, 16 -- Date: Wed, 27 Jul 2005 16:19:30 -0700 5, 16 -- To: MSA listserver {microscopy-at-microscopy.com} 5, 16 -- From: Gary Gaugler {gary-at-gaugler.com} 5, 16 -- Subject: Trolling for SEM/STEM specimens 5, 16 -- Mime-Version: 1.0 5, 16 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 16 -- X-TST: smtp2.mc.surewest.net SNWK3 0.31-80 ip=66.60.171.211 ==============================End of - Headers==============================
isn't the golden rule do uuto others before they do unto you? which clearly seems to be the case in a preemptive attack
--- frank.karl-at-degussa.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } } Folks, lets remenber the Golden Rule with our } dealing with the list } server.... } } The man with the gold get to make the rules. Simple } is it not? } } Frank Karl } Degussa Corporation } Akron Technical Center } 3500 Embassy Parkway } Suite 100 } Akron, Ohio 44333 } } } 330-668-2235 Ext. 238 } } } This e-mail transmission, and any documents, files } or previous e-mail } messages attached to it may contain information that } is confidential or } legally privileged. If you are not the intended } recipient, or a person } responsible for delivering it to the intended } recipient, you are hereby } notified that you must not read this transmission } and that any disclosure, } copying, printing, distribution or use of any of the } information contained } in or attached to this transmission is STRICTLY } PROHIBITED. If you have } received this transmission in error, please } immediately notify the sender } by telephone or return e-mail and delete the } original transmission and its } attachments without reading or saving in any manner. } Thank you. } } } ==============================Original } Headers============================== } 12, 17 -- From frank.karl-at-degussa.com Wed Jul 27 } 11:46:02 2005 } 12, 17 -- Received: from framailout1.rz.itson.com } (mailout2.degussa.com [149.216.91.173]) } 12, 17 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6RGjvV1030393 } 12, 17 -- for {microscopy-at-msa.microscopy.com} ; Wed, } 27 Jul 2005 11:46:02 -0500 } 12, 17 -- Received: from } mobuscomm01.mail.degussa.com ([172.20.6.74]) } 12, 17 -- by framailout1.rz.itson.com } (8.13.3/8.13.3/Debian-6) with ESMTP id } j6RGiW5s027918 } 12, 17 -- for {microscopy-at-msa.microscopy.com} ; Wed, } 27 Jul 2005 18:45:20 +0200 } 12, 17 -- Subject: golden rule applies here } 12, 17 -- To: microscopy-at-msa.microscopy.com } 12, 17 -- X-Mailer: Lotus Notes Release 6.5.2 June } 01, 2004 } 12, 17 -- Message-ID: } {OF7928F80A.6C45314D-ON8525704B.005DDFD5-8525704B.005E1722-at-degussa.com} } 12, 17 -- From: frank.karl-at-degussa.com } 12, 17 -- Date: Wed, 27 Jul 2005 13:07:43 -0400 } 12, 17 -- X-MIMETrack: Serialize by Router on } MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, } 2004) at } 12, 17 -- 07/27/2005 11:45:41 AM } 12, 17 -- MIME-Version: 1.0 } 12, 17 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - } Headers============================== }
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
This is a scientific forum, tirelessly, patiently, and good-humoredly run for the benefit of us all by Nestor, who certainly doesn't deserve this, not some sort of internet chat room.
Stop sniping or quit the list.
rtch
Date sent: Wed, 27 Jul 2005 19:32:16 -0500 To: r.sims-at-auckland.ac.nz X-from: hoffpajo-at-yahoo.com Send reply to: microscopy-at-microscopy.com
Note to Winston et al: I see nothing in the listserver FAQ that says a revoked subscriber can't request to be resubscribed.
I have a suspicion that much of this brouhaha can be sourced to record heat stewing the brains of hapless millions in the Lower 48 and southern Canada.
Hawaii sounds just perfect right about now. Y'all enjoy! The rest of us will stand in front of the refrigerator with the door open ;^)
Libby Shaw
} Date: Wed, 27 Jul 2005 10:49:35 -0500 } From: Winston.Wiggins-at-cshs.org } Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- **************************************************************** Elisabeth L. Shaw, Facility Coordinator Surface and Spectroscopy Labs Analytical Shared Experimental Facilities MIT Center for Materials Science and Engineering
Address: MIT Room 13-4149 Tel: 617-253-5045 77 Massachusetts Avenue Email: elshaw-at-mit.edu Cambridge, MA 02139 Fax: 617-258-6478 http://web.mit.edu/cmse/www/ ****************************************************************
==============================Original Headers============================== 9, 24 -- From elshaw-at-MIT.EDU Wed Jul 27 22:35:21 2005 9, 24 -- Received: from biscayne-one-station.mit.edu (BISCAYNE-ONE-STATION.MIT.EDU [18.7.7.80]) 9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6S3ZKZg004448 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 22:35:21 -0500 9, 24 -- Received: from outgoing.mit.edu (OUTGOING-AUTH.MIT.EDU [18.7.22.103]) 9, 24 -- by biscayne-one-station.mit.edu (8.12.4/8.9.2) with ESMTP id j6S3WsIg019830 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 23:32:54 -0400 (EDT) 9, 24 -- Received: from [18.82.0.176] (PEABODY.MIT.EDU [18.82.0.176]) 9, 24 -- (authenticated bits=0) 9, 24 -- (User authenticated as elshaw-at-ATHENA.MIT.EDU) 9, 24 -- by outgoing.mit.edu (8.13.1/8.12.4) with ESMTP id j6S3Wi5B016974 9, 24 -- (version=TLSv1/SSLv3 cipher=RC4-SHA bits=128 verify=NOT) 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 23:32:46 -0400 (EDT) 9, 24 -- Mime-Version: 1.0 9, 24 -- X-Sender: elshaw-at-po11.mit.edu 9, 24 -- Message-Id: {p06100508bf0de6fce8b9-at-[18.82.0.176]} 9, 24 -- Date: Wed, 27 Jul 2005 23:32:44 -0400 9, 24 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 9, 24 -- From: Libby Shaw {elshaw-at-MIT.EDU} 9, 24 -- Subject: RE: Discussion of high IQ vs Disparagement of a Pers 9, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 9, 24 -- X-Spam-Score: -0.596 9, 24 -- X-Spam-Flag: NO 9, 24 -- X-Scanned-By: MIMEDefang 2.42 ==============================End of - Headers==============================
My delete key has certainly had a workout yesterday and today. I do hope that tomorrow brings a respite from the "heat" generated by this thread and that we can all move on with our lives. From personal experience, life can be way too short to expend so much energy on this issue which in the end, I believe, will have advanced the wisdom of humankind not one iota but seems to have created ill feelings among some folks.
Please, if you wish to reply to this message, just reply to me and not the entire list and give this thread and the list a well deserved rest!
Damian Neuberger
==============================Original Headers============================== 7, 16 -- From neuberger1234-at-comcast.net Wed Jul 27 22:55:20 2005 7, 16 -- Received: from rwcrmhc11.comcast.net (rwcrmhc11.comcast.net [204.127.198.35]) 7, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6S3tHTw012352 7, 16 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 22:55:20 -0500 7, 16 -- Received: from 204.127.197.111 (rwcrwbc20.attbi.com[204.127.197.130]) 7, 16 -- by comcast.net (rwcrmhc11) with SMTP 7, 16 -- id {2005072803551801300raa7re} ; Thu, 28 Jul 2005 03:55:18 +0000 7, 16 -- Received: from [12.161.33.195] by 204.127.197.111; 7, 16 -- Thu, 28 Jul 2005 03:55:18 +0000 7, 16 -- From: neuberger1234-at-comcast.net 7, 16 -- To: microscopy-at-microscopy.com 7, 16 -- Subject: Discussion of high IQ thread 7, 16 -- Date: Thu, 28 Jul 2005 03:55:18 +0000 7, 16 -- Message-Id: {072820050355.19570.42E857260000586E00004C722206999735CBCCCDCE9D0A099D0A0D9A0A02-at-comcast.net} 7, 16 -- X-Mailer: AT&T Message Center Version 1 (Dec 17 2004) 7, 16 -- X-Authenticated-Sender: bmV1YmVyZ2VyMTIzNEBjb21jYXN0Lm5ldA== ==============================End of - Headers==============================
The beauty of this board is the ability to disseminate the knowledge of microscopy. This has proven useful to me as well as countless others across the globe.
That being said, I think some of us across this board need to lighten up.
If certain things said here trigger specific emotions personally, then it's time to bring those things up to your personal psychologist, not this listserv.
My trigger finger is getting tired of hitting the delete key to these emotional responses... can't we just get back to microscopy, the purpose of this board??!
- Tracy
On 7/27/05 5:16 PM, "cgarber-at-2spi.com" {cgarber-at-2spi.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } Tony Garratt-Reed wrote: } ======================================================== } I have to say I was waiting for Chuck's response on this thread - my jaw } dropped when I read it, until the explanation came in the next e-mail!!! } ======================================================== } Perhaps I became a bit "gun shy" after sending off a message in error. I } appreciate the understanding of those on the list for someone, like myself, } who did make an error. It has taken me more than a few hours to recover } from that. } } I have operated an independent testing and analytical laboratory with its } core capabilities being built around SEM/EDS and TEM and LM since 1970. I } think I have at least some credentials to speak to the issue at hand. } However, what I have to say might be seen as "too much" for a normal } listserver posting, therefore I have posted my "reply" to URL } http://www.2spi.com/letter.html } } Chuck } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.2spi.com } ######################## } ============================================ } } } } } } } } ==============================Original Headers============================== } 7, 13 -- From cgarber-at-2spi.com Wed Jul 27 17:12:36 2005 } 7, 13 -- Received: from sccrmhc12.comcast.net (sccrmhc12.comcast.net } [204.127.202.56]) } 7, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6RMCZHk026498 } 7, 13 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 27 Jul 2005 17:12:35 -0500 } 7, 13 -- Message-Id: {200507272212.j6RMCZHk026498-at-ns.microscopy.com} } 7, 13 -- Received: from [127.0.0.1] } (pcp02988842pcs.malvrn01.pa.comcast.net[68.85.250.247]) } 7, 13 -- by comcast.net (sccrmhc12) with SMTP } 7, 13 -- id {2005072722123401200a4381e} ; Wed, 27 Jul 2005 22:12:34 } +0000 } 7, 13 -- To: MICROSCOPY BB {Microscopy-at-msa.microscopy.com} } 7, 13 -- Subject: University facilities being used for outside users } 7, 13 -- Date: Wed, 27 Jul 2005 18:12:49 -0500 } 7, 13 -- From: "Garber, Charles A." {cgarber-at-2spi.com} } 7, 13 -- X-Mailer: E-Mail Connection v3.1a } ==============================End of - Headers==============================
----- Tracy E. Anderson Microscopist / Imaging Specialist Imaging Center University of Minnesota Phone: 612.624.3454 Fax: 612.624.1799 http://www.cbs.umn.edu/ic/
³Science and art belong to the whole world, and before them vanish the barriers of nationality.² - Goethe
==============================Original Headers============================== 11, 28 -- From tanderso-at-cbs.umn.edu Thu Jul 28 00:49:09 2005 11, 28 -- Received: from biosci.cbs.umn.edu (biosci.cbs.umn.edu [134.84.144.2]) 11, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6S5n9Rp021551 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 00:49:09 -0500 11, 28 -- Received: from localhost (atp.cbs.umn.edu [134.84.144.139]) 11, 28 -- by biosci-10025.cbs.umn.edu (Postfix) with ESMTP id F362D11608 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 00:47:30 -0500 (CDT) 11, 28 -- Received: from biosci.cbs.umn.edu ([134.84.144.2]) 11, 28 -- by localhost (atp [134.84.144.139]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id 02013-10 for {microscopy-at-microscopy.com} ; 11, 28 -- Thu, 28 Jul 2005 00:49:18 -0500 (CDT) 11, 28 -- Received: from [160.94.80.149] (c94-80-149.cbs.umn.edu [160.94.80.149]) 11, 28 -- by biosci.cbs.umn.edu (Postfix) with ESMTP id A278011607 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 00:47:25 -0500 (CDT) 11, 28 -- User-Agent: Microsoft-Entourage/11.1.0.040913 11, 28 -- Date: Thu, 28 Jul 2005 00:49:15 -0500 11, 28 -- Subject: Re: [Microscopy] University facilities being used for outside 11, 28 -- users 11, 28 -- From: "Tracy E. Anderson" {tanderso-at-cbs.umn.edu} 11, 28 -- To: {microscopy-at-microscopy.com} 11, 28 -- Message-ID: {BF0DDC0B.21BD%tanderso-at-cbs.umn.edu} 11, 28 -- In-Reply-To: {200507272216.j6RMGXYo001049-at-ns.microscopy.com} 11, 28 -- Mime-version: 1.0 11, 28 -- Content-type: text/plain; 11, 28 -- charset="ISO-8859-1" 11, 28 -- X-Virus-Scanned: by amavisd-new-20030616-p10 (Debian) at cbs.umn.edu 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6S5n9Rp021551 ==============================End of - Headers==============================
I realize now that my previous message doesn't directly relate to the subject line. It was mainly in response to the "IQ" post, which I thought was interesting and humorous to read.
Bon soiree
==============================Original Headers============================== 8, 25 -- From tanderso-at-cbs.umn.edu Thu Jul 28 01:00:51 2005 8, 25 -- Received: from biosci.cbs.umn.edu (biosci.cbs.umn.edu [134.84.144.2]) 8, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6S60pQu029329 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 01:00:51 -0500 8, 25 -- Received: from localhost (atp.cbs.umn.edu [134.84.144.139]) 8, 25 -- by biosci-10025.cbs.umn.edu (Postfix) with ESMTP id 7C70011607 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 00:59:27 -0500 (CDT) 8, 25 -- Received: from biosci.cbs.umn.edu ([134.84.144.2]) 8, 25 -- by localhost (atp [134.84.144.139]) (amavisd-new, port 10024) 8, 25 -- with ESMTP id 03726-10 for {microscopy-at-microscopy.com} ; 8, 25 -- Thu, 28 Jul 2005 01:01:15 -0500 (CDT) 8, 25 -- Received: from [160.94.80.149] (c94-80-149.cbs.umn.edu [160.94.80.149]) 8, 25 -- by biosci.cbs.umn.edu (Postfix) with ESMTP id 7642F115FE 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 00:59:23 -0500 (CDT) 8, 25 -- User-Agent: Microsoft-Entourage/11.1.0.040913 8, 25 -- Date: Thu, 28 Jul 2005 01:01:13 -0500 8, 25 -- Subject: Zoinks 8, 25 -- From: "Tracy E. Anderson" {tanderso-at-cbs.umn.edu} 8, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 25 -- Message-ID: {BF0DDED9.21D2%tanderso-at-cbs.umn.edu} 8, 25 -- Mime-version: 1.0 8, 25 -- Content-type: text/plain; 8, 25 -- charset="US-ASCII" 8, 25 -- Content-transfer-encoding: 7bit 8, 25 -- X-Virus-Scanned: by amavisd-new-20030616-p10 (Debian) at cbs.umn.edu ==============================End of - Headers==============================
I will be out of the office starting 07/28/2005 and will not return until 08/10/2005.
I am on vacation and not able to pick up emails or voicemails. If you need immediate help please contact customer service in Bannockburn, otherwise I will respond on August 10th. Thank you. Gretchen
_____________________________________________________________________ This e-mail has been scanned for viruses by MCI's Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.mci.com
==============================Original Headers============================== 8, 30 -- From Gretchen.Ziegler-at-leica-microsystems.com Thu Jul 28 01:01:15 2005 8, 30 -- Received: from mail78.messagelabs.com (mail78.messagelabs.com [195.245.230.131]) 8, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6S61EsO029744 8, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 01:01:15 -0500 8, 30 -- X-VirusChecked: Checked 8, 30 -- X-Env-Sender: Gretchen.Ziegler-at-leica-microsystems.com 8, 30 -- X-Msg-Ref: server-11.tower-78.messagelabs.com!1122530501!30047897!1 8, 30 -- X-StarScan-Version: 5.4.15; banners=leica-microsystems.com,-,- 8, 30 -- X-Originating-IP: [207.242.216.181] 8, 30 -- Received: (qmail 3231 invoked from network); 28 Jul 2005 06:01:41 -0000 8, 30 -- Received: from external.leica-microsystems.com (HELO USDERNS05.e-leica.com) (207.242.216.181) 8, 30 -- by server-11.tower-78.messagelabs.com with SMTP; 28 Jul 2005 06:01:41 -0000 8, 30 -- Received: from usderns01.e-leica.com ([10.30.19.17]) 8, 30 -- by USDERNS05.e-leica.com (Lotus Domino Release 6.5.1) 8, 30 -- with ESMTP id 2005072801013938-1691 ; 8, 30 -- Thu, 28 Jul 2005 01:01:39 -0500 8, 30 -- Subject: Gretchen Ziegler/USDER/West/Leica is out of the office. 8, 30 -- From: Gretchen.Ziegler-at-leica-microsystems.com 8, 30 -- To: microscopy-at-microscopy.com 8, 30 -- Message-ID: {OFCF0BA9DD.D8B20859-ON8625704C.00211C5C-8625704C.00211C5C-at-leica-microsystems.com} 8, 30 -- Date: Thu, 28 Jul 2005 01:01:39 -0500 8, 30 -- MIME-Version: 1.0 8, 30 -- X-MIMETrack: Serialize by Router on USDERNS01/USDER/West/Leica(Release 6.5.1|January 21, 2004) at 8, 30 -- 07/28/2005 01:01:40 AM, 8, 30 -- Itemize by SMTP Server on USDERNS05/USDER/West/Leica(Release 6.5.1|January 8, 30 -- 21, 2004) at 07/28/2005 01:01:39 AM, 8, 30 -- Serialize by Router on USDERNS05/USDER/West/Leica(Release 6.5.1|January 21, 2004) at 8, 30 -- 07/28/2005 01:01:41 AM, 8, 30 -- Serialize complete at 07/28/2005 01:01:41 AM 8, 30 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
You know I just knew you couldn't resist replying the email. First of all there is nothing anonymous about my email. I do believe my name appears in fron of the email address. 2nd I think you refered to yahoo as a freebie email account, while yes it is true I do not pay a cent for the account, I do however pay a very high price for my cable broadband account. How many of you on this list server can say that when they post a message, that has very little to do with work? 3rd you have no clue who you are talking to. I have 25 years in electron microscopy. I did however retire from the field 2 years ago at the ripe old age of 46, mainly to get away from people like you. I do what I want when I want and with whomever I want. Now if you are trying to run off those of us with experience and something to contribute, with those you disagree you are doing a good job. You sir seem to know a lot more about internet chat rooms than I do.
Finally: I leave it to Nestor to decide if your account should be pulled for using DISPARAGING REMARKS directed at me, quoteing you "anonymous bitching!" I suspect he will do nothing. Now having said all that, if you having anything further to say to me on the subject email me directly. I will be happy to explain myself with full vigor. John Hoffpauir ret 637 pine street (society hill) philadelphia pa 19106 PS does this make you happy?
--- r.sims-at-auckland.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } For heaven's sake, stop your anonymous bitching! } } This is a scientific forum, tirelessly, patiently, } and good-humoredly run for the benefit } of us all by Nestor, who certainly doesn't deserve } this, not some sort of internet chat } room. } } Stop sniping or quit the list. } } rtch } } } } } Date sent: Wed, 27 Jul 2005 19:32:16 -0500 } To: r.sims-at-auckland.ac.nz } X-from: hoffpajo-at-yahoo.com } Send reply to: microscopy-at-microscopy.com } Subject: [Microscopy] Re: golden rule } applies here } } } } } } } } } } ---------------------------------------------------------------------- } } ------ The Microscopy ListServer -- CoSponsor: } The Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------- } } ------ } } } } isn't the golden rule do uuto others before they } do } } unto you? } } which clearly seems to be the case in a preemptive } } attack } } } } --- frank.karl-at-degussa.com wrote: } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------- } } ------ } The Microscopy ListServer -- CoSponsor: } The } Microscopy } } Society of America } To Subscribe/Unsubscribe -- } } } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------- } } ------ } } } } } } Folks, lets remenber the Golden } Rule with our } } } dealing with the list } server.... } } The man } with the gold get to } } make the rules. Simple } is it not? } } Frank } Karl } Degussa } } Corporation } Akron Technical Center } 3500 } Embassy Parkway } Suite } } 100 } Akron, Ohio 44333 } } } 330-668-2235 Ext. } 238 } } } This e-mail } } transmission, and any documents, files } or } previous e-mail } messages } } attached to it may contain information that } is } confidential or } } } legally privileged. If you are not the intended } } recipient, or a } } person } responsible for delivering it to the } intended } recipient, } } you are hereby } notified that you must not read } this transmission } } } and that any disclosure, } copying, printing, } distribution or use of } } any of the } information contained } in or } attached to this } } transmission is STRICTLY } PROHIBITED. If you have } } received this } } transmission in error, please } immediately notify } the sender } by } } telephone or return e-mail and delete the } } original transmission and } } its } attachments without reading or saving in any } manner. } Thank } } you. } } } ==============================Original } } } } Headers============================== } 12, 17 -- } From } } frank.karl-at-degussa.com Wed Jul 27 } 11:46:02 2005 } } 12, 17 -- } } Received: from framailout1.rz.itson.com } } (mailout2.degussa.com } } [149.216.91.173]) } 12, 17 -- by } ns.microscopy.com (8.12.11/8.12.8) } } } with ESMTP id j6RGjvV1030393 } 12, 17 -- for } } {microscopy-at-msa.microscopy.com} ; Wed, } 27 Jul } 2005 11:46:02 -0500 } } } 12, 17 -- Received: from } } mobuscomm01.mail.degussa.com } } ([172.20.6.74]) } 12, 17 -- by } framailout1.rz.itson.com } } } (8.13.3/8.13.3/Debian-6) with ESMTP id } } j6RGiW5s027918 } 12, 17 -- } } for {microscopy-at-msa.microscopy.com} ; Wed, } 27 } Jul 2005 18:45:20 } } +0200 } 12, 17 -- Subject: golden rule applies } here } 12, 17 -- To: } } microscopy-at-msa.microscopy.com } 12, 17 -- } X-Mailer: Lotus Notes } } Release 6.5.2 June } 01, 2004 } 12, 17 -- } Message-ID: } } } } {OF7928F80A.6C45314D-ON8525704B.005DDFD5-8525704B.005E1722-at-degussa.com } } } } 12, 17 -- From: frank.karl-at-degussa.com } 12, } 17 -- Date: Wed, 27 } } Jul 2005 13:07:43 -0400 } 12, 17 -- X-MIMETrack: } Serialize by Router } } on } MOBUSComm01/DHexternal/US(Release } 6.5.1|January 21, } 2004) at } } } 12, 17 -- 07/27/2005 11:45:41 AM } 12, 17 -- } MIME-Version: 1.0 } 12, } } 17 -- Content-type: text/plain; charset=US-ASCII } } } ==============================End of - } } } Headers============================== } } } } } } } __________________________________________________ } } Do You Yahoo!? } } Tired of spam? Yahoo! Mail has the best spam } protection around } } http://mail.yahoo.com } } } } ==============================Original } } Headers============================== 5, 19 -- } From hoffpajo-at-yahoo.com } } Wed Jul 27 19:30:42 2005 5, 19 -- Received: from } } web50201.mail.yahoo.com (web50201.mail.yahoo.com } [206.190.38.42]) 5, } } 19 -- by ns.microscopy.com (8.12.11/8.12.8) with
you are correct, i did however find the need to write to this sims guy i note you have one of those anonymous email accout he rails against. john --- neuberger1234-at-comcast.net wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Folks, } } My delete key has certainly had a workout yesterday } and today. I do hope that } tomorrow brings a respite from the "heat" generated } by this thread and that we } can all move on with our lives. From personal } experience, life can be way too } short to expend so much energy on this issue which } in the end, I believe, will } have advanced the wisdom of humankind not one iota } but seems to have created ill } feelings among some folks. } } Please, if you wish to reply to this message, just } reply to me and not the } entire list and give this thread and the list a well } deserved rest! } } Damian Neuberger } } } } } ==============================Original } Headers============================== } 7, 16 -- From neuberger1234-at-comcast.net Wed Jul 27 } 22:55:20 2005 } 7, 16 -- Received: from rwcrmhc11.comcast.net } (rwcrmhc11.comcast.net [204.127.198.35]) } 7, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j6S3tHTw012352 } 7, 16 -- for {microscopy-at-microscopy.com} ; Wed, 27 } Jul 2005 22:55:20 -0500 } 7, 16 -- Received: from 204.127.197.111 } (rwcrwbc20.attbi.com[204.127.197.130]) } 7, 16 -- by comcast.net (rwcrmhc11) with
there is nothing to criticize, Sergey was censored. john from an anaoymous email account
--- Jane.LaGoy-at-bodycote.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was enjoying the IQ discourse when it was } light-hearted, but I don't } believe the Microscopy Listserver, or any other } professional listserver, } should disparage people personally. It is not a } question of censorship but } of human decency. What is it our parents said? "If } you can't say something } nice about someone then don't say anything at all". } The IQ lady publicly } wrote her opinion, so that leaves those words as } open game for criticism. I } think it is unfortunate that Sergey was not allowed } to make his own choice } about leaving the list; in that way, he was } censored. This is my personal } opinion (and since I submitted it, you folks are all } welcome to criticize } it.) } } Jane L. LaGoy } R&D Engineer } Bodycote HIP } 155 River Street } Andover, MA 01810 } } } ==============================Original } Headers============================== } 3, 15 -- From Jane.LaGoy-at-bodycote.com Wed Jul 27 } 08:47:35 2005 } 3, 15 -- Received: from Exchange.BODYCOTE-IMT.COM } (mail.bodycote-imt.com [12.30.23.178]) } 3, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j6RDlYof028763 } 3, 15 -- for {Microscopy-at-MSA.Microscopy.com} ; Wed, } 27 Jul 2005 08:47:35 -0500 } 3, 15 -- Received: by mail.bodycote-imt.com with } Internet Mail Service (5.5.2657.72) } 3, 15 -- id {3Y5JKY1V} ; Wed, 27 Jul 2005 09:48:37 } -0400 } 3, 15 -- Message-ID: } {CBB9714FDC67D411B39400D0B73C4B7302336FD8-at-mail.bodycote-imt.com} } 3, 15 -- From: JLaGoy {Jane.LaGoy-at-bodycote.com} } 3, 15 -- To: "Microscopy Listserver (E-mail)" } {Microscopy-at-MSA.Microscopy.com} } 3, 15 -- Subject: my $0.02 worth on disparagement } 3, 15 -- Date: Wed, 27 Jul 2005 09:48:34 -0400 } 3, 15 -- MIME-Version: 1.0 } 3, 15 -- X-Mailer: Internet Mail Service } (5.5.2657.72) } 3, 15 -- Content-Type: text/plain; } 3, 15 -- charset="iso-8859-1" } ==============================End of - } Headers============================== }
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opps i was trying to send that one directly to the poster, heaven forbit i start a new thread, my apology.
--- hoffpajo-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } there is nothing to criticize, Sergey was censored. } john from an anaoymous email account } } --- Jane.LaGoy-at-bodycote.com wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } I was enjoying the IQ discourse when it was } } light-hearted, but I don't } } believe the Microscopy Listserver, or any other } } professional listserver, } } should disparage people personally. It is not a } } question of censorship but } } of human decency. What is it our parents said? } "If } } you can't say something } } nice about someone then don't say anything at } all". } } The IQ lady publicly } } wrote her opinion, so that leaves those words as } } open game for criticism. I } } think it is unfortunate that Sergey was not } allowed } } to make his own choice } } about leaving the list; in that way, he was } } censored. This is my personal } } opinion (and since I submitted it, you folks are } all } } welcome to criticize } } it.) } } } } Jane L. LaGoy } } R&D Engineer } } Bodycote HIP } } 155 River Street } } Andover, MA 01810 } } } } } } ==============================Original } } Headers============================== } } 3, 15 -- From Jane.LaGoy-at-bodycote.com Wed Jul 27 } } 08:47:35 2005 } } 3, 15 -- Received: from Exchange.BODYCOTE-IMT.COM } } (mail.bodycote-imt.com [12.30.23.178]) } } 3, 15 -- by ns.microscopy.com (8.12.11/8.12.8) } with } } ESMTP id j6RDlYof028763 } } 3, 15 -- for {Microscopy-at-MSA.Microscopy.com} ; } Wed, } } 27 Jul 2005 08:47:35 -0500 } } 3, 15 -- Received: by mail.bodycote-imt.com with } } Internet Mail Service (5.5.2657.72) } } 3, 15 -- id {3Y5JKY1V} ; Wed, 27 Jul 2005 09:48:37 } } -0400 } } 3, 15 -- Message-ID: } } } {CBB9714FDC67D411B39400D0B73C4B7302336FD8-at-mail.bodycote-imt.com} } } 3, 15 -- From: JLaGoy {Jane.LaGoy-at-bodycote.com} } } 3, 15 -- To: "Microscopy Listserver (E-mail)" } } {Microscopy-at-MSA.Microscopy.com} } } 3, 15 -- Subject: my $0.02 worth on disparagement } } 3, 15 -- Date: Wed, 27 Jul 2005 09:48:34 -0400 } } 3, 15 -- MIME-Version: 1.0 } } 3, 15 -- X-Mailer: Internet Mail Service } } (5.5.2657.72) } } 3, 15 -- Content-Type: text/plain; } } 3, 15 -- charset="iso-8859-1" } } ==============================End of - } } Headers============================== } } } } } __________________________________________________ } Do You Yahoo!? } Tired of spam? Yahoo! Mail has the best spam } protection around } http://mail.yahoo.com } } ==============================Original } Headers============================== } 5, 19 -- From hoffpajo-at-yahoo.com Thu Jul 28 07:34:04 } 2005 } 5, 19 -- Received: from web50207.mail.yahoo.com } (web50207.mail.yahoo.com [206.190.38.48]) } 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with
* * * * * * * * * "I'm trying to express in grams the amount of NaOH that I'd need for 100 ml of saturated NaOH in ethanol." * * * * * * * * * (1) I don't know the exact answer off-hand, but my first guess would be 20 grams in 100 ml ethanol.
(2) However, I do have experience of dissolving alkalies in various alcohols to make etchants for micrscopy, and there are some things to watch out for:
(a) there will probably be a sodium carbonate crust which will not dissolve.
(b) the behaviour will be very dependent on the amount of water in the ethanol. In fact, there is a possibility of phase separation into a strong aqueous NaOH solution and the production of sodium ethoxide in the ethanolic layer. Regarding ethanol, I read it on the web, but I do have experience with the same reaction happening in butanol.
*** Of general interest ***
Potassium hydroxide (10% wt/vol) in isopropanol (propan-2-ol) is a jolly good cleaner for getting charred organic residues off metals and glass. But don't let it anywhere near aluminium, or you'll start an involuntary preparation of aluminium isopropoxide.
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
==============================Original Headers============================== 9, 21 -- From hinmeigeng-at-hotmail.com Thu Jul 28 09:13:09 2005 9, 21 -- Received: from hotmail.com (bay101-f40.bay101.hotmail.com [64.4.56.50]) 9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SED9d7016556 9, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 28 Jul 2005 09:13:09 -0500 9, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 9, 21 -- Thu, 28 Jul 2005 07:13:08 -0700 9, 21 -- Message-ID: {BAY101-F403622D5ACFA51D742A5F2CACF0-at-phx.gbl} 9, 21 -- Received: from 64.4.56.209 by by101fd.bay101.hotmail.msn.com with HTTP; 9, 21 -- Thu, 28 Jul 2005 14:13:08 GMT 9, 21 -- X-Originating-IP: [64.4.56.209] 9, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 9, 21 -- X-Sender: hinmeigeng-at-hotmail.com 9, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk 9, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 9, 21 -- To: Microscopy-at-MSA.Microscopy.Com 9, 21 -- Cc: R.H.Olley-at-reading.ac.uk 9, 21 -- Subject: RE: saturated NaOH 9, 21 -- Date: Thu, 28 Jul 2005 14:13:08 +0000 9, 21 -- Mime-Version: 1.0 9, 21 -- Content-Type: text/plain; format=flowed 9, 21 -- X-OriginalArrivalTime: 28 Jul 2005 14:13:08.0757 (UTC) FILETIME=[7AEEF450:01C5937E] ==============================End of - Headers==============================
Does anyone have experience with acetonitrile as a substitute transition solvent for PO, or perhaps as the sole dehydration solvent in TEM prep? I am checking the literature and archives, but any personal experiences would be very useful.
Thanks!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Thu Jul 28 10:36:07 2005 9, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SFa7CC025279 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 10:36:07 -0500 9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Thu, 28 Jul 2005 10:36:06 -0500 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: TEM: dehydration solvents 9, 23 -- Date: Thu, 28 Jul 2005 10:36:05 -0500 9, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE7980321-at-UM-EMAIL09.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: TEM: dehydration solvents 9, 23 -- Thread-Index: AcWTihGD84zrKDPTTpm/P/bUIVuR7Q== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 28 Jul 2005 15:36:06.0653 (UTC) FILETIME=[11FD9AD0:01C5938A] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6SFa7CC025279 ==============================End of - Headers==============================
Randy, I tried dehydrating plant tissue for LM with acetonitrile instead of ethanol and the results were terrible. The tissue was preserved poorly. It was the root of arabidopsis. Tobias
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I'd be interested, too, if you would share responses which aren't posted to the list.
Thanks in advance.
Paula.
Paula Allan-Wojtas Research Scientist - Food Microstructure/Chercheure scientifique, microstructure des aliments Food Safety and Quality Team/Salubrité et qualité des aliments Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada 32 Main Street/ 32 rue Main Kentville, Nova Scotia/Kentville, (Nouvelle-Écosse) Canada B4N 1J5 Telephone/Téléphone: (902) 679-5566 Facsimile/Télécopieur: (902) 679-2311
allanwojtasp-at-agr.gc.ca
Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Thursday, July 28, 2005 12:37 PM To: Allan-Wojtas, Paula
Hi all,
Does anyone have experience with acetonitrile as a substitute transition solvent for PO, or perhaps as the sole dehydration solvent in TEM prep? I am checking the literature and archives, but any personal experiences would be very useful.
Thanks!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Thu Jul 28 10:36:07 2005 9, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SFa7CC025279 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 10:36:07 -0500 9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Thu, 28 Jul 2005 10:36:06 -0500 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: TEM: dehydration solvents 9, 23 -- Date: Thu, 28 Jul 2005 10:36:05 -0500 9, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE7980321-at-UM-EMAIL09.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: TEM: dehydration solvents 9, 23 -- Thread-Index: AcWTihGD84zrKDPTTpm/P/bUIVuR7Q== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 28 Jul 2005 15:36:06.0653 (UTC) FILETIME=[11FD9AD0:01C5938A] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6SFa7CC025279 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 29 -- From AllanWojtasP-at-AGR.GC.CA Thu Jul 28 11:00:02 2005 20, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 20, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SG02MX008854 20, 29 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 11:00:02 -0500 20, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 20, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j6SFxxMC018107 20, 29 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 11:59:59 -0400 20, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 20, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j6SFxwd7001537 20, 29 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:00:02 -0400 20, 29 -- Received: from ONNCRXMS1.AGR.GC.CA ([10.117.15.26]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 20, 29 -- Thu, 28 Jul 2005 11:59:50 -0400 20, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 20, 29 -- content-class: urn:content-classes:message 20, 29 -- MIME-Version: 1.0 20, 29 -- Content-Type: text/plain; 20, 29 -- charset="iso-8859-1" 20, 29 -- Subject: RE: [Microscopy] TEM: dehydration solvents 20, 29 -- Date: Thu, 28 Jul 2005 11:59:45 -0400 20, 29 -- Message-ID: {B1242C783DA1ED47AD6D38D952CDF60ED45E9E-at-onncrxms1.agr.gc.ca} 20, 29 -- X-MS-Has-Attach: 20, 29 -- X-MS-TNEF-Correlator: 20, 29 -- Thread-Topic: [Microscopy] TEM: dehydration solvents 20, 29 -- Thread-Index: AcWTijGnRCgAxHQXTYST2BrbXvwboAAAxHMw 20, 29 -- From: "Allan-Wojtas, Paula" {AllanWojtasP-at-AGR.GC.CA} 20, 29 -- To: {microscopy-at-microscopy.com} 20, 29 -- X-OriginalArrivalTime: 28 Jul 2005 15:59:50.0169 (UTC) FILETIME=[6278E890:01C5938D] 20, 29 -- Content-Transfer-Encoding: 8bit 20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6SG02MX008854 ==============================End of - Headers==============================
I did it too, sent something to the wrong place......I'm sorry. Just getting ready to go on holidays and I acted in haste......
P.
Paula Allan-Wojtas Research Scientist - Food Microstructure/Chercheure scientifique, microstructure des aliments Food Safety and Quality Team/Salubrité et qualité des aliments Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada 32 Main Street/ 32 rue Main Kentville, Nova Scotia/Kentville, (Nouvelle-Écosse) Canada B4N 1J5 Telephone/Téléphone: (902) 679-5566 Facsimile/Télécopieur: (902) 679-2311
allanwojtasp-at-agr.gc.ca
==============================Original Headers============================== 3, 29 -- From AllanWojtasP-at-AGR.GC.CA Thu Jul 28 11:03:55 2005 3, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 3, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SG3tSB014780 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 11:03:55 -0500 3, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 3, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j6SG3tMC018381 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:03:55 -0400 3, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 3, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j6SG3wd3001818 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:03:58 -0400 3, 29 -- Received: from ONNCRXMS1.AGR.GC.CA ([10.117.15.26]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 3, 29 -- Thu, 28 Jul 2005 12:03:49 -0400 3, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 3, 29 -- content-class: urn:content-classes:message 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; 3, 29 -- charset="iso-8859-1" 3, 29 -- Subject: sorry for the post 3, 29 -- Date: Thu, 28 Jul 2005 12:03:49 -0400 3, 29 -- Message-ID: {B1242C783DA1ED47AD6D38D952CDF60ED45EA0-at-onncrxms1.agr.gc.ca} 3, 29 -- X-MS-Has-Attach: 3, 29 -- X-MS-TNEF-Correlator: 3, 29 -- Thread-Topic: sorry for the post 3, 29 -- Thread-Index: AcWTjc5Stu8iOIKOTn2zpUmBlwwHjQ== 3, 29 -- From: "Allan-Wojtas, Paula" {AllanWojtasP-at-AGR.GC.CA} 3, 29 -- To: {microscopy-at-microscopy.com} 3, 29 -- X-OriginalArrivalTime: 28 Jul 2005 16:03:50.0074 (UTC) FILETIME=[F17781A0:01C5938D] 3, 29 -- Content-Transfer-Encoding: 8bit 3, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6SG3tSB014780 ==============================End of - Headers==============================
I've found acetonitrile as a PO substitution, to work very well. Also, it dissolves some plastics less readily, and is better for cell cultures.
The results seem just as good if not better than PO in my situations. I don't even use PO anymore.
On Jul 28, 2005, at 10:37 AM, TindallR-at-missouri.edu wrote: } ---------------------------------------------------------------- } } Hi all, } } Does anyone have experience with acetonitrile as a substitute } transition } solvent for PO, or perhaps as the sole dehydration solvent in TEM prep? } I am checking the literature and archives, but any personal experiences } would be very useful. } } Thanks! } } Randy
==============================Original Headers============================== 8, 18 -- From lamiller-at-uiuc.edu Thu Jul 28 12:34:26 2005 8, 18 -- Received: from expredir6.cites.uiuc.edu (expredir6.cites.uiuc.edu [128.174.5.97]) 8, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SHYPGB025505 8, 18 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:34:25 -0500 8, 18 -- Received: from [130.126.19.3] (tortoise.cvm.uiuc.edu [130.126.19.3]) 8, 18 -- by expredir6.cites.uiuc.edu (8.12.11/8.12.11) with ESMTP id j6SHYPkL008723 8, 18 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:34:25 -0500 (CDT) 8, 18 -- Mime-Version: 1.0 (Apple Message framework v622) 8, 18 -- In-Reply-To: {200507281537.j6SFb5OZ026561-at-ns.microscopy.com} 8, 18 -- References: {200507281537.j6SFb5OZ026561-at-ns.microscopy.com} 8, 18 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 8, 18 -- Message-Id: {27f82f6fdeed8c44f371c06fb936e411-at-uiuc.edu} 8, 18 -- Content-Transfer-Encoding: 7bit 8, 18 -- From: Lou Ann Miller {lamiller-at-uiuc.edu} 8, 18 -- Subject: Re: [Microscopy] TEM: dehydration solvents 8, 18 -- Date: Thu, 28 Jul 2005 12:34:25 -0500 8, 18 -- To: microscopy-at-microscopy.com 8, 18 -- X-Mailer: Apple Mail (2.622) ==============================End of - Headers==============================
Hello, I've been normally using a 120kV TEM with a LaB6 filament to image biological materials. I was using a 200kV tem with a field emission gun recently and had problems with damage to the samples embedded in epon araldite and formvar-C TEM grids. Is it necessary to use a different embedding media with higher emission microscopes? Gordon
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ Gordon Ante Vrdoljak Electron Microscope Lab AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall gvrdolja-at-nature.berkeley.edu UC Berkeley phone (510) 642-2085 Berkeley CA 94720-3330 fax (510) 643-6207 cell (510) 290-6793
==============================Original Headers============================== 2, 24 -- From gvrdolja-at-nature.berkeley.edu Thu Jul 28 14:09:45 2005 2, 24 -- Received: from nature.Berkeley.EDU (nature.Berkeley.EDU [128.32.253.219]) 2, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SJ9j3T017365 2, 24 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 14:09:45 -0500 2, 24 -- Received: from localhost (localhost [127.0.0.1]) 2, 24 -- by nature.Berkeley.EDU (Postfix) with ESMTP id DE55BC1E3F 2, 24 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:09:44 -0700 (PDT) 2, 24 -- Received: from nature.Berkeley.EDU ([127.0.0.1]) 2, 24 -- by localhost (nature.berkeley.edu [127.0.0.1]) (amavisd-new, port 10024) 2, 24 -- with ESMTP id 09615-03 for {microscopy-at-microscopy.com} ; 2, 24 -- Thu, 28 Jul 2005 12:09:39 -0700 (PDT) 2, 24 -- Received: by nature.Berkeley.EDU (Postfix, from userid 7458) 2, 24 -- id ADA30C1E57; Thu, 28 Jul 2005 12:09:39 -0700 (PDT) 2, 24 -- Received: from localhost (localhost [127.0.0.1]) 2, 24 -- by nature.Berkeley.EDU (Postfix) with ESMTP id A7530C1E55 2, 24 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:09:39 -0700 (PDT) 2, 24 -- Date: Thu, 28 Jul 2005 12:09:39 -0700 (PDT) 2, 24 -- From: Gordon Vrololjak {gvrdolja-at-nature.berkeley.edu} 2, 24 -- To: microscopy-at-microscopy.com 2, 24 -- Subject: 200kv TEM and field emission 2, 24 -- Message-ID: {Pine.SOC.4.63.0507281206130.6732-at-nature.Berkeley.EDU} 2, 24 -- MIME-Version: 1.0 2, 24 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 2, 24 -- X-Virus-Scanned: amavisd-new at nature.berkeley.edu ==============================End of - Headers==============================
I apologize for posting to all, but this is the only way we can get this message to the participants in the M&M 2005 golf outing this weekend. The computer of the organizer, Mark Sanders, is taking a vacation!
The bus situation has changed. Buses will *not* pick up participants at the Hawaii Convention Center, but will now pickup at the Sheraton Waikiki at 9:45 am and at the Hyatt Regency at 10:15, expected to get to Koolau Golf Course at about 11:15 for a noon tee time.
There are still a few openings for this outing on Saturday and on Sunday; if you are interested in joing them contact Mark Sanders via cell phone 612-867-5885 or by email when his computer is fixed, msanders-at-cbs.umn.edu
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 19 -- From tina-at-pbrc.hawaii.edu Thu Jul 28 14:38:29 2005 7, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SJcSaH000631 7, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 28 Jul 2005 14:38:28 -0500 7, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id j6SJcPjc011848 7, 19 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 7, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 28 Jul 2005 09:38:26 -1000 (HST) 7, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id j6SJcOpY011845 7, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 28 Jul 2005 09:38:25 -1000 (HST) 7, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 7, 19 -- Date: Thu, 28 Jul 2005 09:38:24 -1000 (HST) 7, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 19 -- X-Sender: tina-at-halia 7, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 7, 19 -- Subject: Bus change for M&M Golf outing 7, 19 -- Message-ID: {Pine.GSO.4.21.0507280931440.11799-100000-at-halia} 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
With you all flitting off to Hawaii could you PLEASE do me a favor and not rub my nose in the fact that ya'll get to go on vacation and I have to stay home. Please don't send the "LISTSERVER" an automatic "out of office reply". I've worn the writing off my delete key this week.
Thanks very much,
Dorrance McLean Intrepid GIRL scientist
Dorrance McLean Microsystems Processing Sandia National Laboratories P.O. Box 969, MS 9401 Livermore, CA 94551-0969 925-294-3551 FAX 3870 Email dmclea-at-sandia.gov
==============================Original Headers============================== 10, 29 -- From dmclea-at-sandia.gov Thu Jul 28 14:48:55 2005 10, 29 -- Received: from MM01SNLNTO.sandia.gov (mm01snlnto.sandia.gov [132.175.109.20]) 10, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SJmsmC016187 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 14:48:55 -0500 10, 29 -- Received: from 132.175.109.1 by MM01SNLNTO.sandia.gov with ESMTP ( 10, 29 -- Tumbleweed MMS SMTP Relay 01 (MMS v5.6.3)); Thu, 28 Jul 2005 13:48:45 10, 29 -- -0600 10, 29 -- X-Server-Uuid: 2C1074A8-2B28-4DE3-9F7D-FF40AE090BA2 10, 29 -- Received: from ES23SNLNT.srn.sandia.gov (ec04snlnt.sandia.gov 10, 29 -- [134.253.164.156] (may be forged)) by mailgate.sandia.gov ( 10, 29 -- 8.13.3/8.13.3) with ESMTP id j6SJmi6n022876 for 10, 29 -- {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 13:48:44 -0600 (MDT) 10, 29 -- x-mimeole: Produced By Microsoft Exchange V6.5.7226.0 10, 29 -- Content-class: urn:content-classes:message 10, 29 -- MIME-Version: 1.0 10, 29 -- Subject: M&M in Hawaii 10, 29 -- Date: Thu, 28 Jul 2005 13:48:43 -0600 10, 29 -- Message-ID: {CA82EF34F71386438BB876223415C48B547A3C-at-ES23SNLNT.srn.sandia.gov} 10, 29 -- X-MS-Has-Attach: 10, 29 -- X-MS-TNEF-Correlator: 10, 29 -- Thread-Topic: M&M in Hawaii 10, 29 -- Thread-Index: AcWTrCgseAwh0t1iQ7mkw3CaQ/6urQAAPeYw 10, 29 -- From: "McLean, Dorrance" {dmclea-at-sandia.gov} 10, 29 -- To: microscopy-at-microscopy.com 10, 29 -- X-WSS-ID: 6EF7E9171AC1645795-01-01 10, 29 -- Content-Type: text/plain; 10, 29 -- charset=us-ascii 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6SJmsmC016187 ==============================End of - Headers==============================
You have described what, IMHO, and probably that of most others, is a model policy for appropriate use of university facilities.
Actually there are, within 100 miles of Philadelphia, probably half a dozen for-profit independent laboratories focused (no pun intended) on microscopy capabilities. Some of them are listed on our URL http://www.2spi.com/catalog/hot-service7.html One of our main competitors for the services is EMSL, right across the river from you in Camden.
There is nothing to my knowledge that says a nonprofit can not offer services per se and receive a payment. But you have to become knowledgeable about the tax code itself, and understand how the different classes of tax- exempt institutions differ. Universities are in a separate category and are restricted to doing those things that enhance educational objectives. When the Congress some years ago recognized that some tax-exempt organizations might engage in business/commercial activities, they enacted the UBIT (unrelated business income tax). It was supposed to be the equalizer that kept competition fair between such organizations (mainly not-for-profits or so-called Section 501-(c)-3 organizations). It never worked very well (for what for some could be interesting reasons), but the point was, UBIT was never applied to those operating under the university exemption because Congress just did not envision that a university ever would be engaging in a business/commercial activity. In other words, even if a university **wanted** to pay tax on some activity, there is absolutely no provision in the current tax code for such a tax to be collected and paid!
The Franklin Institute case, to which you referred, was a special case. To explain what happened would require a posting so long it would probably cause Nestor's filters to see it as SPAM..... But they were a not-for- profit (as opposed to universities in general being non-profits) organization and they literally tried to drive Structure Probe, Inc. out of the marketplace by offering rates for SEM services in the early 1970's so low that no for-profit firm could survive. We were the plaintiff who filed an anti-trust suit against them in 1972.
What did them in, and the main reason why their whole research laboratory is no more is that their management people, at the trial and when under oath, were saying things considerably different from when they were talking informally to the IRS and others, like the newspapers. And the net result was that the Franklin Institute Research Laboratories had to become Franklin Institute Research Laboratories, Inc. And this meant they lost most of the benefits they enjoyed as a not-for-profit tax-exempt organization. Putting it another way, they had to pay the same taxes as any other business, they had to double or triple their charge rates and once they started doing that, they started losing customers, and their financial losses became a hemorrhage (literally) and within a few years, they closed up shop and disappeared.
I have given somewhat of a synopsis as to what happened but presumably the court documents and filings are still part of the public record somewhere and if one was interested, such information should be available should one be interested in learning more.
What made this situation unique is that most institutional administrations when confronted with an errant department engaging in inappropriate activities on such a wholesale scale, would step in and say "whoaaaa" and put a stop to it. But in this case, the officers and managers thought they could literally run a tiny three person company out of money and therefore out of business. They obviously failed, but because of that decision, something like 600+ persons eventually lost their jobs when the FIRL, Inc. closed down. The irony to me was that the officers and managers in the end lost nothing. Those from prestigious Philadelphia industrial firms who sat on their Board of Managers lost nothing. None of them were ever held accountable. They were never punished. They just rode off into the sunset with golden glove handshakes......
So that, in a nut shell, is the Philadelphia Story...... and the demise of the Franklin Institute Research Laboratories.
Chuck
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Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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----- Original Message ----- X-from: Pat Connelly To: microscopy-at-microscopy.com Cc: cgarber-at-2spi.com Sent: Thursday, July 28, 2005 1:34 PM
I will be out of the office from July 28th returning to the office on August 8th, 2005.
==============================Original Headers============================== 1, 16 -- From MAILER-DAEMON-at-jeol.com Thu Jul 28 14:50:42 2005 1, 16 -- Received: from copland.jeol.com (copland.jeol.com [65.215.44.133]) 1, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SJofMO021104 1, 16 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 14:50:41 -0500 1, 16 -- Received: from jeol.com (mail.jeol.com [208.196.228.78]) 1, 16 -- by copland.jeol.com (8.12.9p2/8.12.9) with ESMTP id j6SJodQ7062928 1, 16 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 15:50:39 -0400 (EDT) 1, 16 -- (envelope-from MAILER-DAEMON-at-jeol.com) 1, 16 -- From: "Patricia Corkum" {pcorkum-at-jeol.com} 1, 16 -- Date: Thu, 28 Jul 2005 15:50:39 -0400 1, 16 -- Message-ID: {react-1197630-at-jeol.com} 1, 16 -- X-Autogenerated: Reply 1, 16 -- To: microscopy-at-microscopy.com 1, 16 -- Subject: Re: [Microscopy] University facilities being used for outside users 1, 16 -- In-Reply-To: {200507281950.j6SJoRFj020405-at-ns.microscopy.com} 1, 16 -- X-Spam-Score: (-2.82) ALL_TRUSTED ==============================End of - Headers==============================
On Jul 28, 2005, at 12:09 PM, gvrdolja-at-nature.berkeley.edu wrote:
} I've been normally using a 120kV TEM with a LaB6 filament to image } biological materials. I was using a 200kV tem with a field emission } gun } recently and had problems with damage to the samples embedded in epon } araldite and formvar-C TEM grids. Is it necessary to use a different } embedding media with higher emission microscopes? } Dear Gordon, Unless you were operating the 200 kV FEG at a much higher intensity than the 120 kV LaB6, you shouldn't experience damage problems. One can always use a lower-intensity beam (larger spot size number) and spread the beam over a wide area so that a FEG will deliver a low dose rate to the specimen. If you have an intense beam and a correspondingly short exposure for your image, the beam can do damage during the time that it is on the specimen but the image is not being exposed. To clarify that last sentence, if you're scanning the grid with the LaB6 beam, imaging with a 1 sec exposure, and you experience no significant damage during the (say) 10-20 sec during which you're focussing, framing the image, etc., then you go to a FEG beam with a 0.1 sec exposure, taking the same length of time to focus, etc., you will have exposed your specimen to 10 times the dose, so there may well be unacceptable damage. Otherwise, the 200 kV beam does less damage per unit dose than the 120 kV beam, and there is nothing inherent in the FEG that will increase the damage. Standard techniques for producing plastic-embedded specimens should work as well for the 200 kV FEG as for the 120 kV LaB6. Yours, Bill
==============================Original Headers============================== 5, 26 -- From tivol-at-caltech.edu Thu Jul 28 14:54:21 2005 5, 26 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SJsE1c003023 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 14:54:17 -0500 5, 26 -- Received: from earth-dog (earth-dog [192.168.1.3]) 5, 26 -- by water-ox-postvirus (Postfix) with ESMTP id 2538B26ACFE 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:54:14 -0700 (PDT) 5, 26 -- Received: from water-ox ([192.168.1.10]) 5, 26 -- by earth-dog (MailMonitor for SMTP v1.2.2 ) ; 5, 26 -- Thu, 28 Jul 2005 12:54:13 -0700 (PDT) 5, 26 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 5, 26 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id F189826ACFE 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 12:54:12 -0700 (PDT) 5, 26 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 26 -- In-Reply-To: {200507281909.j6SJ9qGQ017545-at-ns.microscopy.com} 5, 26 -- References: {200507281909.j6SJ9qGQ017545-at-ns.microscopy.com} 5, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 26 -- Message-Id: {03a7d64c99538d104e5307c16ddb1256-at-caltech.edu} 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 26 -- Subject: Re: [Microscopy] 200kv TEM and field emission 5, 26 -- Date: Thu, 28 Jul 2005 12:55:13 -0700 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- X-Mailer: Apple Mail (2.622) 5, 26 -- X-Spam-Status: No, hits=0.0 tagged_above=-100000.0 required=5.0 5, 26 -- X-Spam-Level: ==============================End of - Headers==============================
I care about people and would never use the remark of "I dont care about you". Its a simple polite, compassionate rule that most of us are taught to care for people. My point was it was a poor choice of words. Take out that sentence and I think you'll see Nestor would still make his point without sharing his frustrating remark of caring.
Im not going to add any remarks on the subject of this thread. It's already contorted beyond intent of the original author. My posting was an observation as to how everyone, including the sysop, got carried away with this originally intended "amusement post" and maybe all of us ended up being guilty of stepping on the rules. Again, no reason to publically announce how much you care about someone.
Anonymous posting? What more do you need besides my email address and that Im a member of this list? Let me know what you need to know.
--- r.sims-at-auckland.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear bmollon } } } How on earth do you read Nestor's email as } "derogatory and demeaning"? } } Do you see some equivalence between saying "I don't } care who you are" and } describing someone else as practising "crap } journalism"? } } What color is the sky in your world? } } And how about having the courage to identify } yourself? } } Anonymous postings don't rate very highly with most } people. } } cheers } } rtch } } } } } Date sent: Wed, 27 Jul 2005 09:42:24 -0500 } To: r.sims-at-auckland.ac.nz } X-from: bmollon-at-pacbell.net } Send reply to: microscopy-at-microscopy.com } Subject: [Microscopy] rules } } } } } } } } } } ---------------------------------------------------------------------- } } ------ The Microscopy ListServer -- CoSponsor: } The Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------- } } ------ } } } } Nestor..........since you just used a derogatory } and } } demeaning statement about someone publically using } the } } listserver........are you going to remove yourself } } from the list? I think the "I dont care who you } are" } } could have been left off and your point still } made. } } } } Nestor wrote: } } "Sergy } } } } I don't care who you are or where your from, but I } } insist the rules } } which have } } been established for over a decade are followed by } } all. Since you } } believe you are above the rules so be it. You are } } welcome to } } go elsewhere. } } } } I have canceled your subscription to the } Listserver. } } } } } } Nestor } } Microscopy SysOp" } } } } -- } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } Microanalyst Fax : 64 9 } 3737435 } Department of Geology email : } r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original } Headers============================== } 16, 27 -- From r.sims-at-auckland.ac.nz Wed Jul 27 } 14:27:30 2005 } 16, 27 -- Received: from smtpc.itss.auckland.ac.nz } (mailhost.auckland.ac.nz [130.216.190.13]) } 16, 27 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6RJRTHm010518 } 16, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 } Jul 2005 14:27:29 -0500 } 16, 27 -- Received: from localhost } (localhost.localdomain [127.0.0.1]) } 16, 27 -- by smtpc.itss.auckland.ac.nz (Postfix) } with ESMTP id A72DC34EB2; } 16, 27 -- Thu, 28 Jul 2005 07:27:28 +1200 (NZST) } 16, 27 -- Received: from smtpc.itss.auckland.ac.nz } ([127.0.0.1]) } 16, 27 -- by localhost (smtpc.itss.auckland.ac.nz } [127.0.0.1]) (amavisd-new, port 10024) } 16, 27 -- with ESMTP id 11756-07; Thu, 28 Jul 2005 } 07:27:28 +1200 (NZST) } 16, 27 -- Received: from n9k4q7 } (glg-59-134.glg.auckland.ac.nz [130.216.59.134]) } 16, 27 -- by smtpc.itss.auckland.ac.nz (Postfix) } with ESMTP id 88E0C34E98; } 16, 27 -- Thu, 28 Jul 2005 07:27:28 +1200 (NZST) } 16, 27 -- From: "Ritchie Sims" } {r.sims-at-auckland.ac.nz} } 16, 27 -- Organization: Dept of Geology, Univ of } Auckland } 16, 27 -- To: bmollon-at-pacbell.net, } microscopy-at-microscopy.com } 16, 27 -- Date: Thu, 28 Jul 2005 07:28:45 +1200 } 16, 27 -- MIME-Version: 1.0 } 16, 27 -- Subject: Re: [Microscopy] rules } 16, 27 -- Message-ID: } {42E8892D.19407.13BC6A-at-localhost} } 16, 27 -- Priority: normal } 16, 27 -- In-reply-to: } {200507271442.j6REgOPS014970-at-ns.microscopy.com} } 16, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) } 16, 27 -- Content-type: text/plain; charset=US-ASCII } 16, 27 -- Content-transfer-encoding: 7BIT } 16, 27 -- Content-description: Mail message body } 16, 27 -- X-Virus-Scanned: by amavisd-new at } mailhost.auckland.ac.nz } ==============================End of - } Headers============================== }
If this doesn't stop I'll never buy another JEOL product...pcorkum has filled my inbox with automatic out of office replies! And of course, I'll just get another one bounced form complaining! Sorry...I just had to vent.
Dorrance McLean
-----Original Message----- X-from: pcorkum-at-jeol.com [mailto:pcorkum-at-jeol.com] Sent: Thursday, July 28, 2005 1:35 PM To: McLean, Dorrance
I will be out of the office from July 28th returning to the office on August 8th, 2005.
==============================Original Headers============================== 1, 16 -- From MAILER-DAEMON-at-jeol.com Thu Jul 28 15:34:32 2005 1, 16 -- Received: from copland.jeol.com (copland.jeol.com [65.215.44.133]) 1, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SKYVwd007749 1, 16 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 15:34:31 -0500 1, 16 -- Received: from jeol.com (mail.jeol.com [208.196.228.78]) 1, 16 -- by copland.jeol.com (8.12.9p2/8.12.9) with ESMTP id j6SKYRQ7064079 1, 16 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 16:34:27 -0400 (EDT) 1, 16 -- (envelope-from MAILER-DAEMON-at-jeol.com) 1, 16 -- From: "Patricia Corkum" {pcorkum-at-jeol.com} 1, 16 -- Date: Thu, 28 Jul 2005 16:34:26 -0400 1, 16 -- Message-ID: {react-1197859-at-jeol.com} 1, 16 -- X-Autogenerated: Reply 1, 16 -- To: microscopy-at-microscopy.com 1, 16 -- Subject: Re: [Microscopy] Out of office replies and chat room style posts 1, 16 -- In-Reply-To: {200507282034.j6SKYD22006875-at-ns.microscopy.com} 1, 16 -- X-Spam-Score: (-2.82) ALL_TRUSTED ==============================End of - Headers==============================
==============================Original Headers============================== 11, 31 -- From dmclea-at-sandia.gov Thu Jul 28 15:48:51 2005 11, 31 -- Received: from mm02snlnto.sandia.gov (mm02snlnto.sandia.gov [132.175.109.21]) 11, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SKmoMo021234 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 15:48:51 -0500 11, 31 -- Received: from 132.175.109.1 by MM01SNLNTO.sandia.gov with ESMTP ( 11, 31 -- Tumbleweed MMS SMTP Relay 01 (MMS v5.6.3)); Thu, 28 Jul 2005 14:48:37 11, 31 -- -0600 11, 31 -- X-Server-Uuid: 2C1074A8-2B28-4DE3-9F7D-FF40AE090BA2 11, 31 -- Received: from ES23SNLNT.srn.sandia.gov (ec04snlnt.sandia.gov 11, 31 -- [134.253.164.156] (may be forged)) by mailgate.sandia.gov ( 11, 31 -- 8.13.3/8.13.3) with ESMTP id j6SKmaPH004438 for 11, 31 -- {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 14:48:36 -0600 (MDT) 11, 31 -- x-mimeole: Produced By Microsoft Exchange V6.5.7226.0 11, 31 -- Content-class: urn:content-classes:message 11, 31 -- MIME-Version: 1.0 11, 31 -- Subject: RE: [Microscopy] Re: Out of office replies and chat room style 11, 31 -- posts 11, 31 -- Date: Thu, 28 Jul 2005 14:48:35 -0600 11, 31 -- Message-ID: {CA82EF34F71386438BB876223415C48B547A77-at-ES23SNLNT.srn.sandia.gov} 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- Thread-Topic: [Microscopy] Re: Out of office replies and chat room style 11, 31 -- posts 11, 31 -- Thread-Index: AcWTs+NRC3H9E1LhSoeg0Us83Kh/+QAAQi/g 11, 31 -- From: "McLean, Dorrance" {dmclea-at-sandia.gov} 11, 31 -- To: microscopy-at-microscopy.com 11, 31 -- X-WSS-ID: 6EF79B2F1AC1658722-01-01 11, 31 -- Content-Type: text/plain; 11, 31 -- charset=us-ascii 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6SKmoMo021234 ==============================End of - Headers==============================
My most humble apologies to all! This was a dreadful oversight on my part, and will be corrected as promptly as possible.
I truly am sorry about the confusion.
Most cordially, Patricia Corkum JEOL USA } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America
==============================Original Headers============================== 5, 22 -- From pcorkum-at-jeol.com Thu Jul 28 16:29:09 2005 5, 22 -- Received: from copland.jeol.com (copland.jeol.com [65.215.44.133]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6SLT9XZ029444 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 16:29:09 -0500 5, 22 -- Received: from jeol.com (mail.jeol.com [208.196.228.78]) 5, 22 -- by copland.jeol.com (8.12.9p2/8.12.9) with ESMTP id j6SLT3Q7064892 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 17:29:03 -0400 (EDT) 5, 22 -- (envelope-from pcorkum-at-jeol.com) 5, 22 -- Received: from [151.203.6.38] (account pcorkum-at-jeol.com) 5, 22 -- by jeol.com (CommuniGate Pro WebUser 4.1.8) 5, 22 -- with HTTP id 1198095 for microscopy-at-microscopy.com; Thu, 28 Jul 2005 17:29:03 -0400 5, 22 -- From: "Patricia Corkum" {pcorkum-at-jeol.com} 5, 22 -- Subject: Re: [Microscopy] Out of office replies and chat room style 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- X-Mailer: CommuniGate Pro WebUser Interface v.4.1.8 5, 22 -- Date: Thu, 28 Jul 2005 17:29:03 -0400 5, 22 -- Message-ID: {web-1198095-at-jeol.com} 5, 22 -- In-Reply-To: {200507282049.j6SKnMlU022394-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; charset="ISO-8859-1"; format="flowed" 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-Spam-Score: (-2.82) ALL_TRUSTED ==============================End of - Headers==============================
ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK (RTP), NORTH CAROLINA AREA
We have an immediate need for a full-time Electron Microscope Technician with solid knowledge of maintenance and operation. 3+ years TEM and SEM experience in the maintenance and operations of electron microscopy required.
Send resume and salary requirements to sjeffers-at-dgisrd.com {mailto:sjeffers-at-dgisrd.com} .
==============================Original Headers============================== 5, 20 -- From dbaldwin-at-dgisrd.com Thu Jul 28 19:12:49 2005 5, 20 -- Received: from dgi_md.dgisrd.com (x42071c82.ip.e-nt.net [66.7.28.130]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6T0CleE006514 5, 20 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 19:12:49 -0500 5, 20 -- Subject: TEM/SEM Employment Opportunity 5, 20 -- Date: Thu, 28 Jul 2005 20:12:45 -0400 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="UTF-8" 5, 20 -- Message-ID: {9BF5BACE1B29B94D98B896B48FB52B5108FC8D-at-dgi_md.dgisrd.com} 5, 20 -- X-MS-Has-Attach: 5, 20 -- content-class: urn:content-classes:message 5, 20 -- X-MS-TNEF-Correlator: 5, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.4417.0 5, 20 -- Thread-Topic: TEM/SEM Employment Opportunity 5, 20 -- Thread-Index: AcWT0j7pmdYqTuX/T72bOUR9gQYMkA== 5, 20 -- From: "Diane Baldwin" {dbaldwin-at-dgisrd.com} 5, 20 -- To: {microscopy-at-microscopy.com} 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id j6T0CleE006514 ==============================End of - Headers==============================
Has anyone been able to get the Zeiss/LEO drift correction option to work? I have the license and the dongle. Nevertheless, the feature seems rather dysfunctional...or I don't know how to use it.
Can someone enlighten me about this option? I can really use it for long data capture sessions combined with the AVI capture option.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ferretinmicrowave) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, July 24, 2005 at 14:38:34 ---------------------------------------------------------------------------
Email: ferretinmicrowave Name: Isabel Verde
Organization: University of Pennsylvania
Education: 9-12th Grade High School
Location: Chicago, IL, USA
Question: How do you take an image of a snowflake with an optical microscope? What sort of special techniques and equipment do you need in order to take the picture?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ballardmark-at-gmail.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 28, 2005 at 10:58:06 ---------------------------------------------------------------------------
Email: ballardmark-at-gmail.com Name: Marcello
Organization: None
Education: 9-12th Grade High School
Location: Orlando, Florida
Question: Hi to all,
i am trying to buy the most afforable microscopy, but that will be able to help me with biology and chemistry.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dbaldwin-at-dgisrd.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, July 28, 2005 at 13:35:21 ---------------------------------------------------------------------------
Question: ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK (RTP), NORTH CAROLINA AREA
We have an immediate need for a full-time Electron Microscope Technician with solid knowledge in maintenance and operation. 3+ years TEM and SEM experience in maintenance and operation of electron microscopy required.
Send resume and salary requirements to: sjeffers-at-dgisrd.com.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu)
On Thu, 28 Jul 2005 ferretinmicrowave-at-ns.microscopy.com wrote:
} Email: ferretinmicrowave } Name: Isabel Verde } } Organization: University of Pennsylvania } } Education: 9-12th Grade High School } } Location: Chicago, IL, USA } } Question: How do you take an image of a snowflake with an optical } microscope? What sort of special techniques and equipment do you } need in order to take the picture?
==============================Original Headers============================== 6, 21 -- From wpchan-at-u.washington.edu Thu Jul 28 21:13:00 2005 6, 21 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6T2D01k012995 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 21:13:00 -0500 6, 21 -- Received: from homer10.u.washington.edu (homer10.u.washington.edu [140.142.15.114]) 6, 21 -- by mxout1.cac.washington.edu (8.13.4+UW05.04/8.13.4+UW05.05) with ESMTP id j6T2Cx1Z014520 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 19:12:59 -0700 6, 21 -- Received: from localhost (wpchan-at-localhost) 6, 21 -- by homer10.u.washington.edu (8.13.4+UW05.03/8.13.4+UW05.07) with ESMTP id j6T2CxJT134972 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2005 19:12:59 -0700 6, 21 -- Date: Thu, 28 Jul 2005 19:12:59 -0700 (PDT) 6, 21 -- From: "W. Chan" {wpchan-at-u.washington.edu} 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow 6, 21 -- flake 6, 21 -- In-Reply-To: {200507290209.j6T29dqj001639-at-ns.microscopy.com} 6, 21 -- Message-ID: {Pine.A41.4.61b.0507281912040.390260-at-homer10.u.washington.edu} 6, 21 -- References: {200507290209.j6T29dqj001639-at-ns.microscopy.com} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Below is the result of your feedback form } (NJZFM-ultra-55). It was } submitted by (ballardmark-at-gmail.com) from } http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html } } on Thursday, July 28, 2005 at 10:58:06 } --------------------------------------------------------------------------- } } Email: ballardmark-at-gmail.com } Name: Marcello } } Organization: None } } Education: 9-12th Grade High School } } Location: Orlando, Florida } } Question: Hi to all, } } i am trying to buy the most afforable microscopy, } but that will be } able to help me with biology and chemistry. } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 8, 12 -- From zaluzec-at-microscopy.com Thu Jul 28 } 21:07:01 2005 } 8, 12 -- Received: from [10.0.3.241] } (msdvpn24.msd.anl.gov [130.202.238.88]) } 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j6T26vm7024730 } 8, 12 -- for {microscopy-at-microscopy.com} ; Thu, 28 } Jul 2005 21:06:59 -0500 } 8, 12 -- Mime-Version: 1.0 } 8, 12 -- X-Sender: (Unverified) } 8, 12 -- Message-Id: } {p06020403bf0f3faadca0-at-[10.0.3.241]} } 8, 12 -- Date: Thu, 28 Jul 2005 21:06:57 -0500 } 8, 12 -- To: microscopy-at-microscopy.com } 8, 12 -- From: ballardmark-at-gmail.com (by way of } Ask-A-Microscopist) } 8, 12 -- Subject: AskAMicroscopist: an affordable } student microscope } 8, 12 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== }
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
I'm sure that the enquiring high school student will find the information you have so thoughtfully and kindly supplied to him to be useful, will encourage his interest in microscopy, and will induce in him a new respect for microscopists.
Marcello, please wait a little longer and you may find that other list members may post for you information even more relevant and useful than John's kind reply.
I would try, but what I know about light microscopy could easily be written on a small Post-It note.
cheers
Ritchie Sims Auckland New Zealand
Quoting hoffpajo-at-yahoo.com:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } have you gone through your purchasing dept? } } --- ballardmark-at-gmail.com wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Below is the result of your feedback form } } (NJZFM-ultra-55). It was } } submitted by (ballardmark-at-gmail.com) from } } } http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html } } } } on Thursday, July 28, 2005 at 10:58:06 } } } --------------------------------------------------------------------------- } } } } Email: ballardmark-at-gmail.com } } Name: Marcello } } } } Organization: None } } } } Education: 9-12th Grade High School } } } } Location: Orlando, Florida } } } } Question: Hi to all, } } } } i am trying to buy the most afforable microscopy, } } but that will be } } able to help me with biology and chemistry. } } } }
------------------------------------------------- This mail sent through University of Auckland http://www.auckland.ac.nz/
==============================Original Headers============================== 13, 35 -- From r.sims-at-auckland.ac.nz Fri Jul 29 00:20:20 2005 13, 35 -- Received: from smtpc.itss.auckland.ac.nz (mailhost.auckland.ac.nz [130.216.190.13]) 13, 35 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6T5KJXj031213 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 00:20:20 -0500 13, 35 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 35 -- by smtpc.itss.auckland.ac.nz (Postfix) with ESMTP id 2A5593418C 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 17:20:18 +1200 (NZST) 13, 35 -- Received: from smtpc.itss.auckland.ac.nz ([127.0.0.1]) 13, 35 -- by localhost (smtpc.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 13, 35 -- with ESMTP id 23379-28 for {microscopy-at-microscopy.com} ; 13, 35 -- Fri, 29 Jul 2005 17:20:18 +1200 (NZST) 13, 35 -- Received: from motoko.itss.auckland.ac.nz (motoko.itss.auckland.ac.nz [130.216.191.146]) 13, 35 -- by smtpc.itss.auckland.ac.nz (Postfix) with ESMTP id 1626E3410F 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 17:20:18 +1200 (NZST) 13, 35 -- Received: (from apache-at-localhost) 13, 35 -- by motoko.itss.auckland.ac.nz (8.11.6/8.11.6) id j6T5KIu19469 13, 35 -- for microscopy-at-microscopy.com; Fri, 29 Jul 2005 17:20:18 +1200 13, 35 -- Received: from 194.reserved.callplus.net.nz (194.reserved.callplus.net.nz 13, 35 -- [203.184.38.194]) by webmail.auckland.ac.nz (Horde) with HTTP for 13, 35 -- {rsim007-at-webmail.auckland.ac.nz} ; Fri, 29 Jul 2005 17:20:17 +1200 13, 35 -- Message-ID: {1122614417.ad96727a21b4c-at-webmail.auckland.ac.nz} 13, 35 -- Date: Fri, 29 Jul 2005 17:20:17 +1200 13, 35 -- From: r.sims-at-auckland.ac.nz 13, 35 -- To: microscopy-at-microscopy.com 13, 35 -- Subject: Re: [Microscopy] Re: AskAMicroscopist: an affordable student 13, 35 -- microscope 13, 35 -- References: {200507290228.j6T2SOuZ023582-at-ns.microscopy.com} 13, 35 -- In-Reply-To: {200507290228.j6T2SOuZ023582-at-ns.microscopy.com} 13, 35 -- MIME-Version: 1.0 13, 35 -- Content-Type: text/plain; charset="ISO-8859-1" 13, 35 -- Content-Disposition: inline 13, 35 -- Content-Transfer-Encoding: 7bit 13, 35 -- User-Agent: Internet Messaging Program (IMP) 4.0-cvs 13, 35 -- X-Originating-IP: 203.184.38.194 13, 35 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
Some cameras, such as the Nikon 4500 can image larger snow flakes directly with their macro modes, but individual crystals normally require a microscope or a powerful macro setup. Any stereomicroscope with camera attached will do the job, or you could point a digital camera down the eyepiece of any suitable microscope. Either transmitted brightfield or darkfield illumination will work, and imaging between crossed polarisers can generate interesting colour effects.
The main problem is to prevent the snowflake from melting, so the microscope slide and stage need to be at the same temperature as the snowflake. The simplest way, if not the most comfortable way, to do this is to work outside in the snow-shower, or in an unheated shed at subzero temperature.
Some tips, images and historical background can be had from this site: http://www.its.caltech.edu/~atomic/snowcrystals/
With global warming this winter may be your last opportunity... Wrap up warm Chris
----- Original Message ----- X-from: {ferretinmicrowave-at-ns.microscopy.com} To: {cjeffree-at-staffmail.ed.ac.uk} Sent: Friday, July 29, 2005 3:06 AM
Would the Intel-Mattel microscope with a USB support be available? That for high school could really be affordable.
Berns
==============================Original Headers============================== 4, 36 -- From bbuenaobra-at-nip.upd.edu.ph Fri Jul 29 06:27:19 2005 4, 36 -- Received: from mail01.up.edu.ph (ns01.up.edu.ph [202.92.128.248]) 4, 36 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6TBRGlM018725 4, 36 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 06:27:18 -0500 4, 36 -- Received: (qmail 16091 invoked by uid 510); 29 Jul 2005 20:16:13 -0000 4, 36 -- Received: from 10.16.3.34 by web1 (envelope-from {bbuenaobra-at-nip.upd.edu.ph} , uid 501) with qmail-scanner-1.25 4, 36 -- (clamav 29 Jul 2005 10-07 +0200 4, 36 -- Clear:RC:1(10.16.3.34):. 4, 36 -- Processed in 5.757952 secs); 29 Jul 2005 20:16:13 -0000 4, 36 -- Received: from unknown (HELO web4.up.edu.ph) (10.16.3.34) 4, 36 -- by 10.16.3.142 with SMTP; 29 Jul 2005 20:16:07 -0000 4, 36 -- Received: (qmail 21549 invoked by uid 1008); 29 Jul 2005 11:17:31 -0000 4, 36 -- Received: from 10.32.148.2 by web4 (envelope-from {bbuenaobra-at-nip.upd.edu.ph} , uid 1002) with qmail-scanner-1.25 4, 36 -- (clamav 13 Jun 2004 19-55 +0200 4, 36 -- Clear:RC:1(10.32.148.2):. 4, 36 -- Processed in 0.75881 secs); 29 Jul 2005 11:17:31 -0000 4, 36 -- Received: from unknown (HELO cali.nip.upd.edu.ph) (-at-10.32.148.2) 4, 36 -- by 10.16.3.34 with SMTP; 29 Jul 2005 11:17:30 -0000 4, 36 -- Received: from 77f4ls1redg9sgn (virgin.instru.nip.upd.edu.ph [192.168.101.52]) 4, 36 -- by cali.nip.upd.edu.ph (8.8.4/8.8.4) with ESMTP 4, 36 -- id TAA26212 for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 19:30:08 +0800 4, 36 -- From: "Berns Buenaobra" {bbuenaobra-at-nip.upd.edu.ph} 4, 36 -- To: {microscopy-at-microscopy.com} 4, 36 -- Subject: RE: [Microscopy] AskAMicroscopist: an affordable student 4, 36 -- Date: Fri, 29 Jul 2005 19:31:03 -0700 4, 36 -- Message-ID: {002701c594ae$bb6b7810$3465a8c0-at-77f4ls1redg9sgn} 4, 36 -- MIME-Version: 1.0 4, 36 -- Content-Type: text/plain; 4, 36 -- charset="us-ascii" 4, 36 -- Content-Transfer-Encoding: 7bit 4, 36 -- X-Priority: 3 (Normal) 4, 36 -- X-MSMail-Priority: Normal 4, 36 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 4, 36 -- In-Reply-To: {200507290524.j6T5OYDw005527-at-ns.microscopy.com} 4, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 4, 36 -- Importance: Normal ==============================End of - Headers==============================
Check out the Surplus Shed at www.surplusshed.com. They have both dissecting stereomicroscope and compound microscope for $ 95.00 each. I can't vouch for their quality since I've not bought one, but the price is right, and I'm satisfied with other things I've purchased from them. Do NOT buy a discount chain store microscope. They're junk.
Paul Grover
------------------------------------------------------------------------ No matter how much cats fight, there always seem to be plenty of kittens. - A. Lincoln
==============================Original Headers============================== 7, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 08:25:10 2005 7, 24 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 7, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TDPAPJ027508 7, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 08:25:10 -0500 7, 24 -- Received: from paklabpgrover (dhcp155-220.bio.purdue.edu [128.210.155.220]) 7, 24 -- by mailhub130.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j6TDP7X6028966 7, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 08:25:07 -0500 7, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 7, 24 -- To: {microscopy-at-microscopy.com} 7, 24 -- Subject: re: An affordable student microscope 7, 24 -- Date: Fri, 29 Jul 2005 08:25:07 -0500 7, 24 -- Message-ID: {000001c59440$f04a5bf0$dc9bd280-at-paklabpgrover} 7, 24 -- MIME-Version: 1.0 7, 24 -- Content-Type: text/plain; 7, 24 -- charset="us-ascii" 7, 24 -- X-Priority: 3 (Normal) 7, 24 -- X-MSMail-Priority: Normal 7, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 7, 24 -- Importance: Normal 7, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 7, 24 -- X-PMX-Version: 4.7.1.128075 7, 24 -- X-PerlMx-Virus-Scanned: Yes 7, 24 -- Content-Transfer-Encoding: 8bit 7, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6TDPAPJ027508 ==============================End of - Headers==============================
You might want to consider making plastic snowflake replicas. Just google 'snowflake replicas' and you'll find a bunch of sites. If you have trouble finding Formvar, contact me and I'll send you some.
I've had great fun coming inside from a snowstorm and passing these around to guests at a party and seeing how long it takes them to figure out that the snowflakes aren't melting indoors.
Paul Grover
------------------------------------------------------------------------ No matter how much cats fight, there always seem to be plenty of kittens. - A. Lincoln
==============================Original Headers============================== 8, 23 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 08:35:58 2005 8, 23 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 8, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TDZwpC002804 8, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 08:35:58 -0500 8, 23 -- Received: from paklabpgrover (dhcp155-220.bio.purdue.edu [128.210.155.220]) 8, 23 -- by mailhub130.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j6TDZwvh029596 8, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 08:35:58 -0500 8, 23 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- Subject: re: how to image a snowflake 8, 23 -- Date: Fri, 29 Jul 2005 08:35:58 -0500 8, 23 -- Message-ID: {000801c59442$741c95f0$dc9bd280-at-paklabpgrover} 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- X-Priority: 3 (Normal) 8, 23 -- X-MSMail-Priority: Normal 8, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 8, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 8, 23 -- Importance: Normal 8, 23 -- X-PMX-Version: 4.7.1.128075 8, 23 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
When I was at Florida State University the I.T. people in the Biology Department placed networked cameras in the student computer labs.
http://www.axis.com/products/cam_210/index.htm
They had them saving a still pictures every 5 seconds to a network server. I think you can capture video as well with the 210 which is linked above.
Neat thing was that the large format poster printer was in view in one lab so you could just check the web address of the camera to see if your poster was done printing without leaving your office.
Once installed we built metal cages to protect them from being stolen.
I have no financial ties to the company above, just a product that I have worked with in the past with good success.
Hope this helps,
Jon Ekman Imaging Technology Group Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign 405 N. Mathews Avenue Urbana, IL 61801 USA Tel: 217-244-6292 Fax: 217-244-6219
-----Original Message----- X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca] Sent: Wednesday, July 27, 2005 1:38 PM To: ekman-at-itg.uiuc.edu
Hi everyone,
We are currently considering adding security cameras in each of our EM labs.
We have a very large number of users, both during working hours and after-hours in SEM and TEM rooms. Just recently, we have had a few incidents where people have damaged the instrument and did not come forward to admit it.
Although we can see who logged on to the computers, you can still damage the instrument without logging in. We believe that a CCTV in each of the labs would be the best way to solve this problem, but unfortunately we are faced with "privacy issues". People do not want to be watched.
Please tell me if your labs use cameras (live or log) or if you found other ways to get around the problem.
Thanks, Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
Although I have no experience with them, I have received a catalog from a company called Walter Products, Inc.based in Ontario. They specialize in scientific equipment, including microscopes, telescopes, and other supplies. Although the catalog doesn't list their prices for microscopes, their telescope prices seem to be amazingly reasonable (i.e., low) and their warranties seem fine.
You might want to contact them. The email is walter1-at-on.aibn.com, and phone is 519-737-7901. Google the name to get their website. I have no connection or experiences with them, but it might be worth a look.
Good luck, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
-----Original Message----- X-from: ballardmark-at-gmail.com [mailto:ballardmark-at-gmail.com] Sent: Thursday, July 28, 2005 9:08 PM To: Tindall, Randy D.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ballardmark-at-gmail.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 28, 2005 at 10:58:06 ------------------------------------------------------------------------ ---
Email: ballardmark-at-gmail.com Name: Marcello
Organization: None
Education: 9-12th Grade High School
Location: Orlando, Florida
Question: Hi to all,
i am trying to buy the most afforable microscopy, but that will be able to help me with biology and chemistry.
And be sure to check out Wilson A. Bentley's life & work. He pioneered snowflake photography a long time ago, and did it the hard way. Amateurs rock!
Paul Grover
------------------------------------------------------------------------ No matter how much cats fight, there always seem to be plenty of kittens. - A. Lincoln
==============================Original Headers============================== 5, 23 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 08:48:35 2005 5, 23 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TDmZu1021697 5, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 08:48:35 -0500 5, 23 -- Received: from paklabpgrover (dhcp155-220.bio.purdue.edu [128.210.155.220]) 5, 23 -- by mailhub130.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j6TDmZiJ030311 5, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 08:48:35 -0500 5, 23 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- Subject: snowflakes 5, 23 -- Date: Fri, 29 Jul 2005 08:48:35 -0500 5, 23 -- Message-ID: {000001c59444$3732a4c0$dc9bd280-at-paklabpgrover} 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Priority: 3 (Normal) 5, 23 -- X-MSMail-Priority: Normal 5, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 5, 23 -- Importance: Normal 5, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 5, 23 -- X-PMX-Version: 4.7.1.128075 5, 23 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
Hava a look at snowcrystals.com it is a site with lots of info and links about snowflakes including photography.
ferretinmicrowave-at-ns.microscopy.com wrote:
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-- -- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
==============================Original Headers============================== 11, 22 -- From gstrout-at-ou.edu Fri Jul 29 08:55:06 2005 11, 22 -- Received: from fourlom.ou.edu (mail.zero.ou.edu [129.15.0.75]) 11, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TDt6pw000902 11, 22 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 08:55:06 -0500 11, 22 -- Received: from ou.edu ([129.15.38.202]) 11, 22 -- by fourlom.ou.edu (Sun Java System Messaging Server 6.1 HotFix 0.01 (built Jun 11, 22 -- 24 2004)) with ESMTP id {0IKE00A006NSBAD0-at-fourlom.ou.edu} for 11, 22 -- microscopy-at-microscopy.com; Fri, 29 Jul 2005 08:55:05 -0500 (CDT) 11, 22 -- Date: Fri, 29 Jul 2005 08:55:05 -0500 11, 22 -- From: Greg Strout {gstrout-at-ou.edu} 11, 22 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow flake 11, 22 -- In-reply-to: {200507290207.j6T27Orx026366-at-ns.microscopy.com} 11, 22 -- To: microscopy-at-microscopy.com 11, 22 -- Cc: ferretinmicrowave-at-ns.microscopy.com 11, 22 -- Message-id: {42EA3539.4000207-at-ou.edu} 11, 22 -- MIME-version: 1.0 11, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 22 -- Content-transfer-encoding: 7BIT 11, 22 -- X-Accept-Language: en-us, en 11, 22 -- References: {200507290207.j6T27Orx026366-at-ns.microscopy.com} 11, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) 11, 22 -- Gecko/20030624 Netscape/7.1 (ax) ==============================End of - Headers==============================
Since most of you apparently are in Hawaii now, I'll waste a little bandwidth and a few KB to vent. I'd like to note that:
(1) This is a MICROSCOPY listserver. This apparently means "looking at little things".
(2) There is apparently no requirement that one must be using the latest multi-probe cutting edge technology, run a multi-user facility, or have a purchasing department, federal grant, or even a job. Our common bond is that we like to "look at little things".
(3) The greatest scientists have been, mostly, AMATEURS (from Latin 'amator', i.e. someone who does something out of love instead of for financial gain). As we celebrate the centenary of Einstein's 'miracle year' which changed our concept of the universe, let's remember that he did this work as an amateur.
(4) In a few years we'll all be toothless curmudgeons and today's schoolkids will be deciding whether Medicare covers immersion oil and cover slips.
(5) I like to "look at little things" but I'm a carpenter by trade. If you don't like that, bite me.
(6) If you aren't reading this it's because you're in Hawaii and I hate you because I'm not.
Paul Grover :0)
------------------------------------------------------------------------ No matter how much cats fight, there always seem to be plenty of kittens. - A. Lincoln
==============================Original Headers============================== 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 09:35:57 2005 15, 24 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TEZvKW008928 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 09:35:57 -0500 15, 24 -- Received: from paklabpgrover (dhcp155-220.bio.purdue.edu [128.210.155.220]) 15, 24 -- by mailhub130.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j6TEZv5i001612 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 09:35:57 -0500 15, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 15, 24 -- To: {microscopy-at-microscopy.com} 15, 24 -- Subject: raison d' etre 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500 15, 24 -- Message-ID: {000101c5944a$d5416290$dc9bd280-at-paklabpgrover} 15, 24 -- MIME-Version: 1.0 15, 24 -- Content-Type: text/plain; 15, 24 -- charset="us-ascii" 15, 24 -- X-Priority: 3 (Normal) 15, 24 -- X-MSMail-Priority: Normal 15, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 15, 24 -- Importance: Normal 15, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 15, 24 -- X-PMX-Version: 4.7.1.128075 15, 24 -- X-PerlMx-Virus-Scanned: Yes 15, 24 -- Content-Transfer-Encoding: 8bit 15, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j6TEZvKW008928 ==============================End of - Headers==============================
very well said, and Hawaii is over rated try St Martins, much better. you must be the one with the Highest IQ in here. john
--- pgrover-at-bilbo.bio.purdue.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Esteemed Microscopists, } } Since most of you apparently are in Hawaii now, I'll } waste a little } bandwidth and a few KB to vent. I'd like to note } that: } } (1) This is a MICROSCOPY listserver. This } apparently means "looking at } little things". } } (2) There is apparently no requirement that one } must be using the latest } multi-probe cutting edge technology, run a } multi-user facility, or have a } purchasing department, federal grant, or even a job. } Our common bond is } that we like to "look at little things". } } (3) The greatest scientists have been, mostly, } AMATEURS (from Latin } 'amator', i.e. someone who does something out of } love instead of for } financial gain). As we celebrate the centenary of } Einstein's 'miracle year' } which changed our concept of the universe, let's } remember that he did this } work as an amateur. } } (4) In a few years we'll all be toothless } curmudgeons and today's } schoolkids will be deciding whether Medicare covers } immersion oil and cover } slips. } } (5) I like to "look at little things" but I'm a } carpenter by trade. If you } don't like that, bite me. } } (6) If you aren't reading this it's because you're } in Hawaii and I hate you } because I'm not. } } } Paul Grover :0) } } } ------------------------------------------------------------------------ } No matter how much cats fight, there always seem to } be plenty of kittens. } - A. } Lincoln } } } } } ==============================Original } Headers============================== } 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul } 29 09:35:57 2005 } 15, 24 -- Received: from mailhub130.itcs.purdue.edu } (mailhub130.itcs.purdue.edu [128.210.5.130]) } 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6TEZvKW008928 } 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 09:35:57 -0500 } 15, 24 -- Received: from paklabpgrover } (dhcp155-220.bio.purdue.edu [128.210.155.220]) } 15, 24 -- by mailhub130.itcs.purdue.edu } (8.13.4/8.13.4/internal-smtp) with ESMTP id } j6TEZv5i001612 } 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 09:35:57 -0500 } 15, 24 -- From: "pgrover" } {pgrover-at-bilbo.bio.purdue.edu} } 15, 24 -- To: {microscopy-at-microscopy.com} } 15, 24 -- Subject: raison d' etre } 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500 } 15, 24 -- Message-ID: } {000101c5944a$d5416290$dc9bd280-at-paklabpgrover} } 15, 24 -- MIME-Version: 1.0 } 15, 24 -- Content-Type: text/plain; } 15, 24 -- charset="us-ascii" } 15, 24 -- X-Priority: 3 (Normal) } 15, 24 -- X-MSMail-Priority: Normal } 15, 24 -- X-Mailer: Microsoft Outlook, Build } 10.0.4510 } 15, 24 -- Importance: Normal } 15, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.2180 } 15, 24 -- X-PMX-Version: 4.7.1.128075 } 15, 24 -- X-PerlMx-Virus-Scanned: Yes } 15, 24 -- Content-Transfer-Encoding: 8bit } 15, 24 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id } j6TEZvKW008928 } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 5, 19 -- From hoffpajo-at-yahoo.com Fri Jul 29 10:20:16 2005 5, 19 -- Received: from web50206.mail.yahoo.com (web50206.mail.yahoo.com [206.190.38.47]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6TFKGch017078 5, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:20:16 -0500 5, 19 -- Received: (qmail 7618 invoked by uid 60001); 29 Jul 2005 15:20:10 -0000 5, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 19 -- s=s1024; d=yahoo.com; 5, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 19 -- b=zzB1ofrOtbqsavm15j18zAGK7LAlBNbqrAuMg/j1QwaWjSb3sVzpSaw9kQrnFRywjtrYNkZH75hQuaW1FBHSmefYfYH23yBiAnFTMbXSz3MxLWkAsLiPUcG9vMrrKCTwgwV1neM4rs0hnsJ5MADMVXWmBZpia+2nQ3MG2tOr6Lc= ; 5, 19 -- Message-ID: {20050729152010.7616.qmail-at-web50206.mail.yahoo.com} 5, 19 -- Received: from [68.32.53.160] by web50206.mail.yahoo.com via HTTP; Fri, 29 Jul 2005 08:20:10 PDT 5, 19 -- Date: Fri, 29 Jul 2005 08:20:10 -0700 (PDT) 5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com} 5, 19 -- Subject: Re: [Microscopy] raison d' etre 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- In-Reply-To: {200507291437.j6TEbevE011473-at-ns.microscopy.com} 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: text/plain; charset=iso-8859-1 5, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
very well said, and Hawaii is over rated try St Martins, much better. you must be the one with the Highest IQ in here. john
--- pgrover-at-bilbo.bio.purdue.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Esteemed Microscopists, } } Since most of you apparently are in Hawaii now, I'll } waste a little } bandwidth and a few KB to vent. I'd like to note } that: } } (1) This is a MICROSCOPY listserver. This } apparently means "looking at } little things". } } (2) There is apparently no requirement that one } must be using the latest } multi-probe cutting edge technology, run a } multi-user facility, or have a } purchasing department, federal grant, or even a job. } Our common bond is } that we like to "look at little things". } } (3) The greatest scientists have been, mostly, } AMATEURS (from Latin } 'amator', i.e. someone who does something out of } love instead of for } financial gain). As we celebrate the centenary of } Einstein's 'miracle year' } which changed our concept of the universe, let's } remember that he did this } work as an amateur. } } (4) In a few years we'll all be toothless } curmudgeons and today's } schoolkids will be deciding whether Medicare covers } immersion oil and cover } slips. } } (5) I like to "look at little things" but I'm a } carpenter by trade. If you } don't like that, bite me. } } (6) If you aren't reading this it's because you're } in Hawaii and I hate you } because I'm not. } } } Paul Grover :0) } } } ------------------------------------------------------------------------ } No matter how much cats fight, there always seem to } be plenty of kittens. } - A. } Lincoln } } } } } ==============================Original } Headers============================== } 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul } 29 09:35:57 2005 } 15, 24 -- Received: from mailhub130.itcs.purdue.edu } (mailhub130.itcs.purdue.edu [128.210.5.130]) } 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6TEZvKW008928 } 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 09:35:57 -0500 } 15, 24 -- Received: from paklabpgrover } (dhcp155-220.bio.purdue.edu [128.210.155.220]) } 15, 24 -- by mailhub130.itcs.purdue.edu } (8.13.4/8.13.4/internal-smtp) with ESMTP id } j6TEZv5i001612 } 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 09:35:57 -0500 } 15, 24 -- From: "pgrover" } {pgrover-at-bilbo.bio.purdue.edu} } 15, 24 -- To: {microscopy-at-microscopy.com} } 15, 24 -- Subject: raison d' etre } 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500 } 15, 24 -- Message-ID: } {000101c5944a$d5416290$dc9bd280-at-paklabpgrover} } 15, 24 -- MIME-Version: 1.0 } 15, 24 -- Content-Type: text/plain; } 15, 24 -- charset="us-ascii" } 15, 24 -- X-Priority: 3 (Normal) } 15, 24 -- X-MSMail-Priority: Normal } 15, 24 -- X-Mailer: Microsoft Outlook, Build } 10.0.4510 } 15, 24 -- Importance: Normal } 15, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.2180 } 15, 24 -- X-PMX-Version: 4.7.1.128075 } 15, 24 -- X-PerlMx-Virus-Scanned: Yes } 15, 24 -- Content-Transfer-Encoding: 8bit } 15, 24 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id } j6TEZvKW008928 } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 6, 19 -- From hoffpajo-at-yahoo.com Fri Jul 29 10:20:54 2005 6, 19 -- Received: from web50201.mail.yahoo.com (web50201.mail.yahoo.com [206.190.38.42]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6TFKsZv018574 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:20:54 -0500 6, 19 -- Received: (qmail 41665 invoked by uid 60001); 29 Jul 2005 15:20:53 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 19 -- b=Xz+Q6vda7iSW0D5wF20lWd+bQsRRAq0ctjWdf4gwpvrZu8xwaZ9wpo7qxl9dz2ifmaW4c/+sPjun1GmrM1Xm9UPEZz2Jj7U0Yj4qPm0K2iGiVLJemmBXabps/e4DrDUqTlEtt/P4Q0geaw2Rah4yh8jnk0JG/o7SGMGEO5Q3wbI= ; 6, 19 -- Message-ID: {20050729152053.41663.qmail-at-web50201.mail.yahoo.com} 6, 19 -- Received: from [68.32.53.160] by web50201.mail.yahoo.com via HTTP; Fri, 29 Jul 2005 08:20:53 PDT 6, 19 -- Date: Fri, 29 Jul 2005 08:20:53 -0700 (PDT) 6, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com} 6, 19 -- Subject: Re: [Microscopy] raison d' etre 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- In-Reply-To: {200507291437.j6TEbevE011473-at-ns.microscopy.com} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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==============================Original Headers============================== 7, 20 -- From microbill-at-mohawk.net Fri Jul 29 10:37:48 2005 7, 20 -- Received: from mta9.srv.hcvlny.cv.net (mta9.srv.hcvlny.cv.net [167.206.4.204]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TFbl7O032450 7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:37:48 -0500 7, 20 -- Received: from bill.mohawk.net (ool-4356cf00.dyn.optonline.net [67.86.207.0]) 7, 20 -- by mta9.srv.hcvlny.cv.net 7, 20 -- (Sun Java System Messaging Server 6.2-2.06 (built May 11 2005)) 7, 20 -- with ESMTP id {0IKE00GRGBEKKQKF-at-mta9.srv.hcvlny.cv.net} for 7, 20 -- microscopy-at-microscopy.com; Fri, 29 Jul 2005 11:37:33 -0400 (EDT) 7, 20 -- Date: Fri, 29 Jul 2005 11:37:33 -0400 7, 20 -- From: Bill Miller {microbill-at-mohawk.net} 7, 20 -- Subject: Re: [Microscopy] Re: raison d' etre 7, 20 -- In-reply-to: {200507291521.j6TFLHr7020030-at-ns.microscopy.com} 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- Message-id: {6.2.3.4.2.20050729113705.024ffe28-at-mail.mohawk.net} 7, 20 -- MIME-version: 1.0 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 7, 20 -- Content-type: text/plain; charset=us-ascii; format=flowed 7, 20 -- Content-transfer-encoding: 7BIT 7, 20 -- References: {200507291521.j6TFLHr7020030-at-ns.microscopy.com} ==============================End of - Headers==============================
I find myself perplexed by the reason for Paul Gover’s note. I typically check titles and skim the content of the note if I’m interested. Everything else gets trashed canned, so I haven’t been paying too much attention to the discussion. If my lurking finds a theme I’m interested in, I’m not above kicking the embers to bring forth light and heat. With this in mind….
I’ve been making a reasonable living as a microscopist for over 25 years and I still consider myself a very fortunate amateur. I too like to look at little things and to that end built a home microscopy lab while I was in college. I consider the time spent studying diatoms, collecting pollen or cutting free hand thin section of multi-layer bottles to be golden. I find that these skills are transferable to the professional arena.
My first position in a multi-used environment was in the tire industry. Conversations over coffee revealed none of my new co-workers owned a home lab. My microscopy culture tells me all microscopists have a home lab. I knew, at that moment, my co-workers were simply employees and nothing more. All of the microscopist I admire have a home lab and several have created an employment and provided for the welfare of their family from it. Some find their professional lives do not provide the luxury of using the home lab and most regret it.
If amateurs are not welcome on this list, I suggest a name change. I have found this server a useful tool, but I’ll hate to see it restricted to only “profession†microscopist.
Frank Karl Degussa Corporation
. ----- Forwarded by Frank Karl/AKR/Degussa-Huels/US on 07/29/2005 11:34 AM -----
pgrover-at-bilbo.bio .purdue.edu To: frank.karl-at-degussa.com cc: 07/29/2005 10:37 Subject: [Microscopy] raison d' etre AM Please respond to microscopy
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Esteemed Microscopists,
Since most of you apparently are in Hawaii now, I'll waste a little bandwidth and a few KB to vent. I'd like to note that:
(1) This is a MICROSCOPY listserver. This apparently means "looking at little things".
(2) There is apparently no requirement that one must be using the latest multi-probe cutting edge technology, run a multi-user facility, or have a purchasing department, federal grant, or even a job. Our common bond is that we like to "look at little things".
(3) The greatest scientists have been, mostly, AMATEURS (from Latin 'amator', i.e. someone who does something out of love instead of for financial gain). As we celebrate the centenary of Einstein's 'miracle year' which changed our concept of the universe, let's remember that he did this work as an amateur.
(4) In a few years we'll all be toothless curmudgeons and today's schoolkids will be deciding whether Medicare covers immersion oil and cover slips.
(5) I like to "look at little things" but I'm a carpenter by trade. If you don't like that, bite me.
(6) If you aren't reading this it's because you're in Hawaii and I hate you because I'm not.
Paul Grover :0)
------------------------------------------------------------------------ No matter how much cats fight, there always seem to be plenty of kittens. - A. Lincoln
==============================Original Headers============================== 38, 19 -- From frank.karl-at-degussa.com Fri Jul 29 10:38:10 2005 38, 19 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 38, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TFc49t000367 38, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri, 29 Jul 2005 10:38:10 -0500 38, 19 -- Received: from mobuscomm01.mail.degussa.com ([172.20.6.74]) 38, 19 -- by framailout1.rz.itson.com (8.13.3/8.13.3/Debian-6) with ESMTP id j6TFapLj006130 38, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri, 29 Jul 2005 17:37:17 +0200 38, 19 -- Subject: Fw: [Microscopy] raison d' etre 38, 19 -- To: microscopy-at-msa.microscopy.com 38, 19 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 38, 19 -- Message-ID: {OFA5E2B331.F5B85357-ON8525704D.005583B8-8525704D.0055C455-at-degussa.com} 38, 19 -- From: frank.karl-at-degussa.com 38, 19 -- Date: Fri, 29 Jul 2005 11:36:48 -0400 38, 19 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 38, 19 -- 07/29/2005 10:37:45 AM 38, 19 -- MIME-Version: 1.0 38, 19 -- Content-type: text/plain; charset=UTF-8 38, 19 -- Content-Transfer-Encoding: 8bit 38, 19 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id j6TFc49t000367 ==============================End of - Headers==============================
Why perpetuate the myth of the amateur scientist doing his job just for the love of science and not for money? As far as I know, Einstein made a pretty good living for himself while teaching at Princeton, and I'm sure that great scientists before him, like Louis Pasteur, were not poor either! I like many others on this listserver have chosen microscopy as a career, as a way to make a living, and the fact that the "little things" that we look at every day are so beautiful is just a bonus to our jobs! We're doing science a disservice if we tell schoolkids that, if they want to make a living, they should not consider a scientific career!
But like you, I'm pretty jealous of all those going to Hawaii this weekend!
Marc
On Friday, July 29, 2005, at 10:37 AM, pgrover-at-bilbo.bio.purdue.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Esteemed Microscopists, } } Since most of you apparently are in Hawaii now, I'll waste a little } bandwidth and a few KB to vent. I'd like to note that: } } (1) This is a MICROSCOPY listserver. This apparently means "looking } at } little things". } } (2) There is apparently no requirement that one must be using the } latest } multi-probe cutting edge technology, run a multi-user facility, or } have a } purchasing department, federal grant, or even a job. Our common bond } is } that we like to "look at little things". } } (3) The greatest scientists have been, mostly, AMATEURS (from Latin } 'amator', i.e. someone who does something out of love instead of for } financial gain). As we celebrate the centenary of Einstein's 'miracle } year' } which changed our concept of the universe, let's remember that he did } this } work as an amateur. } } (4) In a few years we'll all be toothless curmudgeons and today's } schoolkids will be deciding whether Medicare covers immersion oil and } cover } slips. } } (5) I like to "look at little things" but I'm a carpenter by trade. } If you } don't like that, bite me. } } (6) If you aren't reading this it's because you're in Hawaii and I } hate you } because I'm not. } } } Paul Grover :0) } } } ----------------------------------------------------------------------- } - } No matter how much cats fight, there always seem to be plenty of } kittens. } - A. Lincoln } } } } } ==============================Original } Headers============================== } 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 09:35:57 2005 } 15, 24 -- Received: from mailhub130.itcs.purdue.edu } (mailhub130.itcs.purdue.edu [128.210.5.130]) } 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j6TEZvKW008928 } 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 09:35:57 } -0500 } 15, 24 -- Received: from paklabpgrover (dhcp155-220.bio.purdue.edu } [128.210.155.220]) } 15, 24 -- by mailhub130.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) } with ESMTP id j6TEZv5i001612 } 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 09:35:57 } -0500 } 15, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} } 15, 24 -- To: {microscopy-at-microscopy.com} } 15, 24 -- Subject: raison d' etre } 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500 } 15, 24 -- Message-ID: {000101c5944a$d5416290$dc9bd280-at-paklabpgrover} } 15, 24 -- MIME-Version: 1.0 } 15, 24 -- Content-Type: text/plain; } 15, 24 -- charset="us-ascii" } 15, 24 -- X-Priority: 3 (Normal) } 15, 24 -- X-MSMail-Priority: Normal } 15, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 } 15, 24 -- Importance: Normal } 15, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 } 15, 24 -- X-PMX-Version: 4.7.1.128075 } 15, 24 -- X-PerlMx-Virus-Scanned: Yes } 15, 24 -- Content-Transfer-Encoding: 8bit } 15, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j6TEZvKW008928 } ==============================End of - } Headers============================== } }
-- Marc Pypaert Department of Cell Biology Center for Cell and Molecular Imaging Ludwig Institute for Cancer Research Yale University School of Medicine 333 Cedar Street, PO Box 208002 New Haven, CT 06520-8002 TEL 203-785 3681 FAX 203-785 7446
==============================Original Headers============================== 9, 18 -- From marc.pypaert-at-yale.edu Fri Jul 29 10:49:02 2005 9, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48]) 9, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TFn10n021022 9, 18 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:49:02 -0500 9, 18 -- Received: from yale.edu (net234-111.med.yale.edu [130.132.234.111]) 9, 18 -- by biomed.med.yale.edu (PMDF V6.1-1 #30532) 9, 18 -- with ESMTP id {01LR72VA80WA00C028-at-biomed.med.yale.edu} for 9, 18 -- microscopy-at-microscopy.com; Fri, 29 Jul 2005 11:45:47 -0400 (EDT) 9, 18 -- Date: Fri, 29 Jul 2005 11:45:25 -0400 9, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu} 9, 18 -- Subject: Re: [Microscopy] raison d' etre 9, 18 -- In-reply-to: {200507291437.j6TEb26R010667-at-ns.microscopy.com} 9, 18 -- To: microscopy-at-microscopy.com 9, 18 -- Message-id: {C7EAB472-0047-11DA-AC3D-0030659833B4-at-yale.edu} 9, 18 -- MIME-version: 1.0 (Apple Message framework v553) 9, 18 -- X-Mailer: Apple Mail (2.553) 9, 18 -- Content-type: text/plain; format=flowed; charset=US-ASCII; delsp=yes 9, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
well to be honest, most of us have a life after work, and after 25 years of 12 hour days it gets a little old. you know just upkeeping our lives can be a full time job, not trying to kick anything up either. which is one of the reasons i retired early.
--- frank.karl-at-degussa.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } } I find myself perplexed by the reason for Paul } Gover’s note. I typically } check titles and skim the content of the note if } I’m interested. } Everything else gets trashed canned, so I haven’t } been paying too much } attention to the discussion. If my lurking finds a } theme I’m interested } in, I’m not above kicking the embers to bring } forth light and heat. With } this in mind…. } } } I’ve been making a reasonable living as a } microscopist for over 25 years } and I still consider myself a very fortunate } amateur. I too like to look } at little things and to that end built a home } microscopy lab while I was in } college. I consider the time spent studying } diatoms, collecting pollen or } cutting free hand thin section of multi-layer } bottles to be golden. I find } that these skills are transferable to the } professional arena. } } My first position in a multi-used environment was in } the tire industry. } Conversations over coffee revealed none of my new } co-workers owned a home } lab. My microscopy culture tells me all } microscopists have a home lab. I } knew, at that moment, my co-workers were simply } employees and nothing more. } All of the microscopist I admire have a home lab and } several have created } an employment and provided for the welfare of their } family from it. Some } find their professional lives do not provide the } luxury of using the home } lab and most regret it. } } If amateurs are not welcome on this list, I suggest } a name change. I have } found this server a useful tool, but I’ll hate to } see it restricted to only } “profession†microscopist. } } Frank Karl } Degussa Corporation } } } . } ----- Forwarded by Frank Karl/AKR/Degussa-Huels/US } on 07/29/2005 11:34 AM } ----- } } } } pgrover-at-bilbo.bio } } } .purdue.edu To: } frank.karl-at-degussa.com } } cc: } } } 07/29/2005 10:37 } Subject: [Microscopy] raison d' etre } } AM } } } Please respond to } } } microscopy } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Esteemed Microscopists, } } Since most of you apparently are in Hawaii now, I'll } waste a little } bandwidth and a few KB to vent. I'd like to note } that: } } (1) This is a MICROSCOPY listserver. This } apparently means "looking at } little things". } } (2) There is apparently no requirement that one } must be using the latest } multi-probe cutting edge technology, run a } multi-user facility, or have a } purchasing department, federal grant, or even a job. } Our common bond is } that we like to "look at little things". } } (3) The greatest scientists have been, mostly, } AMATEURS (from Latin } 'amator', i.e. someone who does something out of } love instead of for } financial gain). As we celebrate the centenary of } Einstein's 'miracle } year' } which changed our concept of the universe, let's } remember that he did this } work as an amateur. } } (4) In a few years we'll all be toothless } curmudgeons and today's } schoolkids will be deciding whether Medicare covers } immersion oil and cover } slips. } } (5) I like to "look at little things" but I'm a } carpenter by trade. If } you } don't like that, bite me. } } (6) If you aren't reading this it's because you're } in Hawaii and I hate } you } because I'm not. } } } Paul Grover :0) } } } ------------------------------------------------------------------------ } No matter how much cats fight, there always seem to } be plenty of kittens. } - A. } Lincoln } } } } } } } ==============================Original } Headers============================== } 38, 19 -- From frank.karl-at-degussa.com Fri Jul 29 } 10:38:10 2005 } 38, 19 -- Received: from framailout1.rz.itson.com } (mailout2.degussa.com [149.216.91.173]) } 38, 19 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6TFc49t000367 } 38, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri, } 29 Jul 2005 10:38:10 -0500 } 38, 19 -- Received: from } mobuscomm01.mail.degussa.com ([172.20.6.74]) } 38, 19 -- by framailout1.rz.itson.com } (8.13.3/8.13.3/Debian-6) with ESMTP id } j6TFapLj006130 } 38, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri, } 29 Jul 2005 17:37:17 +0200 } 38, 19 -- Subject: Fw: [Microscopy] raison d' etre } 38, 19 -- To: microscopy-at-msa.microscopy.com } 38, 19 -- X-Mailer: Lotus Notes Release 6.5.2 June } 01, 2004 } 38, 19 -- Message-ID: } {OFA5E2B331.F5B85357-ON8525704D.005583B8-8525704D.0055C455-at-degussa.com} } === message truncated ===
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==============================Original Headers============================== 5, 19 -- From hoffpajo-at-yahoo.com Fri Jul 29 10:50:55 2005 5, 19 -- Received: from web50203.mail.yahoo.com (web50203.mail.yahoo.com [206.190.38.44]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6TFosEa025821 5, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:50:54 -0500 5, 19 -- Received: (qmail 67008 invoked by uid 60001); 29 Jul 2005 15:50:54 -0000 5, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 19 -- s=s1024; d=yahoo.com; 5, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 19 -- b=gSNuv3P0PwmHo/P6fhaAyufvhMWl7gWDXHFEipI8ZAb+wfEVsGaNAyF2Ch9EFU9Yv1K/gXH0YE2FH+9Av+c2yPQtVnoRU3A8rGqx0WLHWCSVGgBjT88Y7HHPJC6fXJmKMVhcX/V5SCgYgzCc3Az3qcZvBGhKHxGob/TYPLFr9rU= ; 5, 19 -- Message-ID: {20050729155054.67006.qmail-at-web50203.mail.yahoo.com} 5, 19 -- Received: from [68.32.53.160] by web50203.mail.yahoo.com via HTTP; Fri, 29 Jul 2005 08:50:54 PDT 5, 19 -- Date: Fri, 29 Jul 2005 08:50:54 -0700 (PDT) 5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com} 5, 19 -- Subject: Re: [Microscopy] Fw: raison d' etre 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- In-Reply-To: {200507291539.j6TFdf7p005133-at-ns.microscopy.com} 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: text/plain; charset=iso-8859-1 5, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
you do have a sense of humor after all, i was begining to wonder. what a nice sardonic reply. finlly get it i do have a name huh?
--- r.sims-at-auckland.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Well said, John! } } I'm sure that the enquiring high school student will } find the information you } have so thoughtfully and kindly supplied to him to } be useful, will encourage his } interest in microscopy, and will induce in him a new } respect for microscopists. } } Marcello, please wait a little longer and you may } find that other list members } may post for you information even more relevant and } useful than John's kind reply. } } I would try, but what I know about light microscopy } could easily be written on a } small Post-It note. } } cheers } } Ritchie Sims } Auckland } New Zealand } } } Quoting hoffpajo-at-yahoo.com: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } have you gone through your purchasing dept? } } } } --- ballardmark-at-gmail.com wrote: } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } } } Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help } } } } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } Below is the result of your feedback form } } } (NJZFM-ultra-55). It was } } } submitted by (ballardmark-at-gmail.com) from } } } } } } http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html } } } } } } on Thursday, July 28, 2005 at 10:58:06 } } } } } } --------------------------------------------------------------------------- } } } } } } Email: ballardmark-at-gmail.com } } } Name: Marcello } } } } } } Organization: None } } } } } } Education: 9-12th Grade High School } } } } } } Location: Orlando, Florida } } } } } } Question: Hi to all, } } } } } } i am trying to buy the most afforable } microscopy, } } } but that will be } } } able to help me with biology and chemistry. } } } } } } } } } } ------------------------------------------------- } This mail sent through University of Auckland } http://www.auckland.ac.nz/ } } ==============================Original } Headers============================== } 13, 35 -- From r.sims-at-auckland.ac.nz Fri Jul 29 } 00:20:20 2005 } 13, 35 -- Received: from smtpc.itss.auckland.ac.nz } (mailhost.auckland.ac.nz [130.216.190.13]) } 13, 35 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6T5KJXj031213 } 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 00:20:20 -0500 } 13, 35 -- Received: from localhost } (localhost.localdomain [127.0.0.1]) } 13, 35 -- by smtpc.itss.auckland.ac.nz (Postfix) } with ESMTP id 2A5593418C } 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 17:20:18 +1200 (NZST) } 13, 35 -- Received: from smtpc.itss.auckland.ac.nz } ([127.0.0.1]) } 13, 35 -- by localhost (smtpc.itss.auckland.ac.nz } [127.0.0.1]) (amavisd-new, port 10024) } 13, 35 -- with ESMTP id 23379-28 for } {microscopy-at-microscopy.com} ; } 13, 35 -- Fri, 29 Jul 2005 17:20:18 +1200 (NZST) } 13, 35 -- Received: from motoko.itss.auckland.ac.nz } (motoko.itss.auckland.ac.nz [130.216.191.146]) } 13, 35 -- by smtpc.itss.auckland.ac.nz (Postfix) } with ESMTP id 1626E3410F } 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 17:20:18 +1200 (NZST) } 13, 35 -- Received: (from apache-at-localhost) } 13, 35 -- by motoko.itss.auckland.ac.nz } (8.11.6/8.11.6) id j6T5KIu19469 } 13, 35 -- for microscopy-at-microscopy.com; Fri, 29 } Jul 2005 17:20:18 +1200 } 13, 35 -- Received: from } 194.reserved.callplus.net.nz } (194.reserved.callplus.net.nz } 13, 35 -- [203.184.38.194]) by } webmail.auckland.ac.nz (Horde) with HTTP for } 13, 35 -- {rsim007-at-webmail.auckland.ac.nz} ; Fri, 29 } Jul 2005 17:20:17 +1200 } 13, 35 -- Message-ID: } {1122614417.ad96727a21b4c-at-webmail.auckland.ac.nz} } 13, 35 -- Date: Fri, 29 Jul 2005 17:20:17 +1200 } 13, 35 -- From: r.sims-at-auckland.ac.nz } 13, 35 -- To: microscopy-at-microscopy.com } 13, 35 -- Subject: Re: [Microscopy] Re: } AskAMicroscopist: an affordable student } 13, 35 -- microscope } 13, 35 -- References: } {200507290228.j6T2SOuZ023582-at-ns.microscopy.com} } 13, 35 -- In-Reply-To: } {200507290228.j6T2SOuZ023582-at-ns.microscopy.com} } 13, 35 -- MIME-Version: 1.0 } 13, 35 -- Content-Type: text/plain; } charset="ISO-8859-1" } 13, 35 -- Content-Disposition: inline } 13, 35 -- Content-Transfer-Encoding: 7bit } 13, 35 -- User-Agent: Internet Messaging Program } (IMP) 4.0-cvs } 13, 35 -- X-Originating-IP: 203.184.38.194 } 13, 35 -- X-Virus-Scanned: by amavisd-new at } mailhost.auckland.ac.nz } ==============================End of - } Headers============================== }
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more "amateur" snowflake pics, 6 web pages beginning at http://cammer.net/blog/snowflake.htm and ending with a movie at http://cammer.net/blog/snowflake06.htm
Also a favorite at http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm
At 08:49 AM 07/29/05 -0500, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/ **This electronic transmission contains information that is privileged.**
==============================Original Headers============================== 12, 23 -- From cammer-at-aecom.yu.edu Fri Jul 29 11:32:11 2005 12, 23 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) 12, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6TGWAKj021272 12, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 11:32:10 -0500 12, 23 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) 12, 23 -- by mailgw.aecom.yu.edu (8.12.11/8.12.11) with SMTP id j6TGVwtj013945 12, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 12:31:58 -0400 12, 23 -- Received: from post.aecom.yu.edu ([129.98.1.100]) 12, 23 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2005072912315801109 12, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 12:31:58 -0400 12, 23 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.30.137]) 12, 23 -- by post.aecom.yu.edu (Postfix) with ESMTP id 9F5232FC0 12, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 12:31:58 -0400 (EDT) 12, 23 -- Message-Id: {5.2.1.1.2.20050729122747.03248008-at-mailserver.aecom.yu.edu} 12, 23 -- X-Sender: cammer-at-mailserver.aecom.yu.edu 12, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 12, 23 -- Date: Fri, 29 Jul 2005 12:29:56 -0400 12, 23 -- To: microscopy-at-microscopy.com 12, 23 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 12, 23 -- Subject: Re: [Microscopy] snowflakes 12, 23 -- In-Reply-To: {200507291349.j6TDnovA026834-at-ns.microscopy.com} 12, 23 -- Mime-Version: 1.0 12, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
On Jul 28, 2005, at 7:06 PM, ferretinmicrowave-at-ns.microscopy.com wrote:
} Question: How do you take an image of a snowflake with an optical } microscope? What sort of special techniques and equipment do you } need in order to take the picture? } Dear Isabel, There is a wonderful book by Dr. Ken Libbrecht called The Snowflake, which has some information about how the incredible images in the book were taken. Basically, he took a microscope slide to collect the flakes as they fell and kept everything cold while providing appropriate illumination for microphotography. If you need details not provided in the book, I'm pretty sure that Ken would be willing to help you out. I can give you his contact info off-list if you want. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 27 -- From tivol-at-caltech.edu Fri Jul 29 12:13:30 2005 5, 27 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6THDU4n004674 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 12:13:30 -0500 5, 27 -- Received: from wood-dog (wood-dog [192.168.1.7]) 5, 27 -- by fire-ox-postvirus (Postfix) with ESMTP id 4101235462 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:13:30 -0700 (PDT) 5, 27 -- Received: from fire-ox ([192.168.1.31]) 5, 27 -- by wood-dog (MailMonitor for SMTP v1.2.2 ) ; 5, 27 -- Fri, 29 Jul 2005 10:13:29 -0700 (PDT) 5, 27 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 5, 27 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 90B9235416 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:13:29 -0700 (PDT) 5, 27 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 27 -- In-Reply-To: {200507290206.j6T26SuT023734-at-ns.microscopy.com} 5, 27 -- References: {200507290206.j6T26SuT023734-at-ns.microscopy.com} 5, 27 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 27 -- Message-Id: {3fa2a2c1ed039863c0be8c316600c45b-at-caltech.edu} 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 27 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow flake 5, 27 -- Date: Fri, 29 Jul 2005 10:14:29 -0700 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- X-Mailer: Apple Mail (2.622) 5, 27 -- X-Virus-Scanned: by amavisd-new at caltech.edu 5, 27 -- X-Spam-Status: No, hits=0.0 tagged_above=-100000.0 required=5.0 5, 27 -- X-Spam-Level: ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (msimms-at-tracelabs.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, July 29, 2005 at 10:06:10 ---------------------------------------------------------------------------
Email: msimms-at-tracelabs.com Name: Michael Simms
Organization: Trace Laboratories - Central
Title-Subject: [Filtered] Measurement of ink depth/contrast
Question: Hello Gentlemen, I work for an independent testing organization, Trace Laboratories - Central. We have been asked to have printing ink measured for depth and contrast. Laser printing is done on the insulation sleeve of a cable. per Sikorsky specification SS7333, Paragraph 4.6.5 This asks for measuring to an accuracy of 0.0001 inch with a contour projector or calibrated microscope or Zygo measuring device. The contrast measurement is suggested to be made with a Spectrum Technology CMS^2 Contrast Measuring System. I would need evidence of either an accredited laboratory or calibration to pass on to the customer.
Is this something that someone associated with this resource might be able to quote?
Regards, Mike
Mike Simms Chemist Trace Laboratories - Central 1150 W. Euclid Ave. Palatine, IL 60067
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (aetmicro-at-optonline.net) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, July 29, 2005 at 13:07:28 ---------------------------------------------------------------------------
Email: aetmicro-at-optonline.net Name: andrew thelian
I have been having a problem with my beam stability currently... I operate a Philips EM300... and in an attempt to regain stability i cleaned the contacts of the high tension line that runs from the gun to the high voltage tank... (the cleaning included the insulators as well)
My questions are... What kind of oil do I use inside the insulators? (i was advised to use Santovac 5 and would like verification because of its cost) What kind of oil should I use for the High Voltage Tank?
Any links to locate vendors would be of great help.
on 7/29/05 2:14 PM, aetmicro-at-optonline.net at aetmicro-at-optonline.net wrote:
} Santovac 5
The Santovac oil is for the diffusion pump *NOT* the high tension tank. I use to know that info. but it has gone poof. I would call FEI they took over Philips or the changed their name they would know.
I read that as well... being that Santovac 5 is made for diffusion pumps... I wasn't going to use it for the high voltage tank... but for the high tension line insulators from the gun to the tank...
I believe its extremely low water content is what makes it a good choice for the insulators... to prevent arcing...
Thank you for your help I am very appreciative:), Andrew
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Dear Bill: A very complete theses on how to do it and how it was done please see Historical Microsopical Society of Canada Bulletin Volume 14 No 55 February 2003 Most of the paper dealt with snow crystals / snow flakes. Alas the Historical Microsopical Society of Canada is no more it is part of the Microscope Historical Society. Hope this helps. Regards, Keith
-----Original Message----- X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] Sent: Friday, July 29, 2005 1:17 PM To: kgmnrc-at-hotmail.com
On Jul 28, 2005, at 7:06 PM, ferretinmicrowave-at-ns.microscopy.com wrote:
} Question: How do you take an image of a snowflake with an optical } microscope? What sort of special techniques and equipment do you } need in order to take the picture? } Dear Isabel, There is a wonderful book by Dr. Ken Libbrecht called The Snowflake,
which has some information about how the incredible images in the book were taken. Basically, he took a microscope slide to collect the flakes as they fell and kept everything cold while providing appropriate illumination for microphotography. If you need details not provided in the book, I'm pretty sure that Ken would be willing to help you out. I can give you his contact info off-list if you want. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 27 -- From tivol-at-caltech.edu Fri Jul 29 12:13:30 2005 5, 27 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6THDU4n004674 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 12:13:30 -0500 5, 27 -- Received: from wood-dog (wood-dog [192.168.1.7]) 5, 27 -- by fire-ox-postvirus (Postfix) with ESMTP id 4101235462 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:13:30 -0700 (PDT) 5, 27 -- Received: from fire-ox ([192.168.1.31]) 5, 27 -- by wood-dog (MailMonitor for SMTP v1.2.2 ) ; 5, 27 -- Fri, 29 Jul 2005 10:13:29 -0700 (PDT) 5, 27 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 5, 27 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 90B9235416 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2005 10:13:29 -0700 (PDT) 5, 27 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 27 -- In-Reply-To: {200507290206.j6T26SuT023734-at-ns.microscopy.com} 5, 27 -- References: {200507290206.j6T26SuT023734-at-ns.microscopy.com} 5, 27 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 27 -- Message-Id: {3fa2a2c1ed039863c0be8c316600c45b-at-caltech.edu} 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 27 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow flake 5, 27 -- Date: Fri, 29 Jul 2005 10:14:29 -0700 5, 7 -- To: microscopy-at-microscopy.com 5, 27 -- X-Mailer: Apple Mail (2.622) 5, 27 -- X-Virus-Scanned: by amavisd-new at caltech.edu 5, 27 -- X-Spam-Status: No, hits=0.0 tagged_above=-100000.0 required=5.0 5, 27 -- X-Spam-Level: ==============================End of - Headers==============================
==============================Original Headers============================== 13, 29 -- From kgmnrc-at-hotmail.com Sat Jul 30 07:51:15 2005 13, 29 -- Received: from hotmail.com (bay105-dav3.bay105.hotmail.com [65.54.224.75]) 13, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6UCpEIo022841 13, 29 -- for {microscopy-at-microscopy.com} ; Sat, 30 Jul 2005 07:51:14 -0500 13, 29 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 13, 29 -- Sat, 30 Jul 2005 05:51:13 -0700 13, 29 -- Message-ID: {BAY105-DAV369B71A5CF300715020B4D1C10-at-phx.gbl} 13, 29 -- Received: from 65.54.224.200 by BAY105-DAV3.phx.gbl with DAV; 13, 29 -- Sat, 30 Jul 2005 12:51:13 +0000 13, 29 -- X-Originating-IP: [65.54.224.200] 13, 29 -- X-Originating-Email: [kgmnrc-at-hotmail.com] 13, 29 -- X-Sender: kgmnrc-at-hotmail.com 13, 29 -- From: "Keith" {kgmnrc-at-hotmail.com} 13, 29 -- To: {microscopy-at-microscopy.com} 13, 29 -- Subject: RE: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow flake 13, 29 -- Date: Sat, 30 Jul 2005 08:50:50 -0400 13, 29 -- MIME-Version: 1.0 13, 29 -- Content-Type: text/plain; 13, 29 -- charset="us-ascii" 13, 29 -- Content-Transfer-Encoding: 7bit 13, 29 -- X-Priority: 1 (Highest) 13, 29 -- X-MSMail-Priority: High 13, 29 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 13, 29 -- In-Reply-To: {200507291716.j6THGUF0010120-at-ns.microscopy.com} 13, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 13, 29 -- Importance: High 13, 29 -- Thread-Index: AcWUYYq4fLQgcLMPQXeSExZblTjBZQAoTC/A 13, 29 -- Content-Class: urn:content-classes:message 13, 29 -- X-OriginalArrivalTime: 30 Jul 2005 12:51:13.0605 (UTC) FILETIME=[5E19C750:01C59505] ==============================End of - Headers==============================
I suspect that transformer oil might do a better job as well as costing far less.
But FEI will have good advice on this, anyway.
cheers
rtch
} } Hi, } } I read that as well... being that Santovac 5 is made for diffusion } pumps... I wasn't going to use it for the high voltage tank... but for } the high tension line insulators from the gun to the tank... } } I believe its extremely low water content is what makes it a good } choice for the insulators... to prevent arcing... } } Thank you for your help I am very appreciative:), } Andrew }
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 7, 27 -- From r.sims-at-auckland.ac.nz Sat Jul 30 15:46:43 2005 7, 27 -- Received: from smtpa.itss.auckland.ac.nz (mailhost.auckland.ac.nz [130.216.190.11]) 7, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6UKkg1u003417 7, 27 -- for {microscopy-at-microscopy.com} ; Sat, 30 Jul 2005 15:46:43 -0500 7, 27 -- Received: from localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) 7, 27 -- by smtpa.itss.auckland.ac.nz (Postfix) with ESMTP id 61F3934C23; 7, 27 -- Sun, 31 Jul 2005 08:46:40 +1200 (NZST) 7, 27 -- Received: from smtpa.itss.auckland.ac.nz ([127.0.0.1]) 7, 27 -- by localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 7, 27 -- with ESMTP id 07198-12; Sun, 31 Jul 2005 08:46:40 +1200 (NZST) 7, 27 -- Received: from n9k4q7 (glg-59-134.glg.auckland.ac.nz [130.216.59.134]) 7, 27 -- by smtpa.itss.auckland.ac.nz (Postfix) with ESMTP id 0E7E0340F0; 7, 27 -- Sun, 31 Jul 2005 08:46:39 +1200 (NZST) 7, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 7, 27 -- Organization: Dept of Geology, Univ of Auckland 7, 27 -- To: aetmicro-at-optonline.net, microscopy-at-microscopy.com 7, 27 -- Date: Sun, 31 Jul 2005 08:47:57 +1200 7, 27 -- MIME-Version: 1.0 7, 27 -- Subject: Santovac for insulation 7, 27 -- Message-ID: {42EC903C.21034.FCF43BF-at-localhost} 7, 27 -- Priority: normal 7, 27 -- In-reply-to: {200507301216.j6UCGeaV017033-at-ns.microscopy.com} 7, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 7, 27 -- Content-type: text/plain; charset=US-ASCII 7, 27 -- Content-transfer-encoding: 7BIT 7, 27 -- Content-description: Mail message body 7, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (stefan.diller-at-t-online.de) from http://microscopy.com/MLFormMail.html on Saturday, July 30, 2005 at 13:43:48 ---------------------------------------------------------------------------
Email: stefan.diller-at-t-online.de Name: Stefan Diller
Question: Dear members, I am looking urgently for a user manual and if possible a service manual or the electronic layout for the Reichert Histostat Paraffin Embedder Modell 8035.
it's probly been over 15 years since i used a 300. but you might want to contact FEI at www.feic.com. if you contact me off line i can give you an email address of someone to talk to directly at FEI. john
--- aetmicro-at-optonline.net wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Below is the result of your feedback form } (NJZFM-ultra-55). It was } submitted by (aetmicro-at-optonline.net) from } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } on } Friday, July 29, 2005 at 13:07:28 } --------------------------------------------------------------------------- } } Email: aetmicro-at-optonline.net } Name: andrew thelian } } Organization: nanoprobes, inc } } Title-Subject: [Filtered] MListserver: unstable beam } } Question: Hi, } } I have been having a problem with my beam stability } currently... I } operate a Philips EM300... and in an attempt to } regain stability i } cleaned the contacts of the high tension line that } runs from the gun } to the high voltage tank... (the cleaning included } the insulators as } well) } } My questions are... What kind of oil do I use inside } the insulators? } (i was advised to use Santovac 5 and would like } verification because } of its cost) What kind of oil should I use for the } High Voltage Tank? } } Any links to locate vendors would be of great help. } } Thank You, } Andy } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 10, 12 -- From zaluzec-at-microscopy.com Fri Jul 29 } 15:10:03 2005 } 10, 12 -- Received: from [10.0.3.241] } (msdvpn26.msd.anl.gov [130.202.238.90]) } 10, 12 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6TK9us6022159 } 10, 12 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 15:09:59 -0500 } 10, 12 -- Mime-Version: 1.0 } 10, 12 -- X-Sender: (Unverified) } 10, 12 -- Message-Id: } {p06020402bf103d6fd0f2-at-[10.0.3.241]} } 10, 12 -- Date: Fri, 29 Jul 2005 15:09:57 -0500 } 10, 12 -- To: microscopy-at-microscopy.com } 10, 12 -- From: aetmicro-at-optonline.net (by way of } MicroscopyListserver) } 10, 12 -- Subject: viaWWW: unstable beam EM300 } 10, 12 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== }
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here is a big a list of web sites as i can compile Snow from eduScapes 42eXplore http://eduscapes.com/42explore/snow.htm Make Snowflakes from KinderArt http://www.kinderart.com/seasons/dec7.shtml Snow http://www.muohio.edu/dragonfly/snow/snow.HTMLX Snow facts http://tqjunior.advanced.org/3876/snowfacts.html Old Camera Photos http://www.flash.net/~bobgil/cam/camera.html Make Your Own Virtual Snowflake http://www.muohio.edu/dragonfly/snow/icensnow.htmlx Edible Snowflakes Recipe http://www.stepbystepcc.com/holidays/christmas5.html Scientists See Snowflakes http://www.ars.usda.gov/is/kids/environment/story2/snowflakeframes.htm
Snowflakes information http://www.macatawa.org/~oias/snowflak.htm Using a Microscope for Snowflakes http://www.microscopy-uk.org.uk/mag/artfeb00/eksnow.html
Wilson A. Bentley --The Snowflake Man http://www.snowflakebentley.com/ Pictures of His Snowflakes http://www.snowflakebentley.com/snowflakes.htm Life of Wilson Bentley (middle grades) http://www.virtualvermont.com/history/sbentley.html Jericho, Vermont http://www.jericho-underhill.com/ Famous People from Vermont http://www.virtualvermont.com/history/people.html Photography from eduScapes 42eXplore http://eduscapes.com/42explore/photog.htm
Educator Links Story of Wilson Bentley Life (Upper level reading) http://www.snowflakebentley.com/sfman.htm http://www.snowflakebentley.com/prior.html Photographing Snowflakes (Upper level reading) http://www.snowflakebentley.com/wbsf.htm Snowflakes- A Thematic Approach http://www.wsanford.com/~wsanford/exo/n-m_snowflakes.html
Further Reading About Ice and Snow http://www.muohio.edu/dragonfly/snow/readings.HTMLX Making a Snowflake (Upper level reading) http://highhopes.com/snowflakes.html History of Photography (Mature/adult) http://home.globalcrossing.net/~sos/history.html History of Photography (Mature/adult) http://www.rleggat.com/photohistory/ Snowflake Bentley http://www.carolhurst.com/titles/snowflakebentley.html
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Bill: } A very complete theses on how to do it and how it } was done please see } Historical Microsopical Society of Canada Bulletin } Volume 14 No 55 February } 2003 Most of the paper dealt with snow crystals / } snow flakes. Alas the } Historical Microsopical Society of Canada is no more } it is part of the } Microscope Historical Society. } Hope this helps. } Regards, } Keith } } -----Original Message----- } X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] } } Sent: Friday, July 29, 2005 1:17 PM } To: kgmnrc-at-hotmail.com } Subject: [Microscopy] Re: [Filtered] } AskAMicroscopist: How to image a snow } flake } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Jul 28, 2005, at 7:06 PM, } ferretinmicrowave-at-ns.microscopy.com wrote: } } } Question: How do you take an image of a snowflake } with an optical } } microscope? What sort of special techniques and } equipment do you } } need in order to take the picture? } } } Dear Isabel, } There is a wonderful book by Dr. Ken Libbrecht } called The Snowflake, } } which has some information about how the incredible } images in the book } were taken. Basically, he took a microscope slide } to collect the } flakes as they fell and kept everything cold while } providing } appropriate illumination for microphotography. If } you need details not } provided in the book, I'm pretty sure that Ken would } be willing to help } you out. I can give you his contact info off-list } if you want. } Yours, } Bill Tivol, PhD } EM Scientist and Manager } Cryo-Electron Microscopy Facility } Broad Center, Mail Code 114-96 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } } ==============================Original } Headers============================== } 5, 27 -- From tivol-at-caltech.edu Fri Jul 29 12:13:30 } 2005 } 5, 27 -- Received: from } outgoing-mail.its.caltech.edu } (outgoing-mail.its.caltech.edu [131.215.239.19]) } 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id } j6THDU4n004674 } 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 12:13:30 } -0500 } 5, 27 -- Received: from wood-dog (wood-dog } [192.168.1.7]) } 5, 27 -- by fire-ox-postvirus (Postfix) with ESMTP } id 4101235462 } 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 10:13:30 } -0700 (PDT) } 5, 27 -- Received: from fire-ox ([192.168.1.31]) } 5, 27 -- by wood-dog (MailMonitor for SMTP v1.2.2 ) } ; } 5, 27 -- Fri, 29 Jul 2005 10:13:29 -0700 (PDT) } 5, 27 -- Received: from [192.168.157.234] } (pix-1.caltech.edu [131.215.2.21]) } 5, 27 -- by fire-ox.its.caltech.edu (Postfix) with } ESMTP id } 90B9235416 } 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 } Jul 2005 10:13:29 } -0700 (PDT) } 5, 27 -- Mime-Version: 1.0 (Apple Message framework } v622) } 5, 27 -- In-Reply-To: } {200507290206.j6T26SuT023734-at-ns.microscopy.com} } 5, 27 -- References: } {200507290206.j6T26SuT023734-at-ns.microscopy.com} } 5, 27 -- Content-Type: text/plain; charset=US-ASCII; } format=flowed } 5, 27 -- Message-Id: } {3fa2a2c1ed039863c0be8c316600c45b-at-caltech.edu} } 5, 27 -- Content-Transfer-Encoding: 7bit } 5, 27 -- From: Bill Tivol {tivol-at-caltech.edu} } 5, 27 -- Subject: Re: [Microscopy] [Filtered] } AskAMicroscopist: How to image } a snow flake } 5, 27 -- Date: Fri, 29 Jul 2005 10:14:29 -0700 } 5, 7 -- To: microscopy-at-microscopy.com } 5, 27 -- X-Mailer: Apple Mail (2.622) } 5, 27 -- X-Virus-Scanned: by amavisd-new at } caltech.edu } 5, 27 -- X-Spam-Status: No, hits=0.0 } tagged_above=-100000.0 required=5.0 } 5, 27 -- X-Spam-Level: } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 13, 29 -- From kgmnrc-at-hotmail.com Sat Jul 30 } 07:51:15 2005 } 13, 29 -- Received: from hotmail.com } (bay105-dav3.bay105.hotmail.com [65.54.224.75]) } 13, 29 -- by ns.microscopy.com (8.12.11/8.12.8) } with ESMTP id j6UCpEIo022841 } 13, 29 -- for {microscopy-at-microscopy.com} ; Sat, 30 } Jul 2005 07:51:14 -0500 } 13, 29 -- Received: from mail pickup service by } hotmail.com with Microsoft SMTPSVC; } 13, 29 -- Sat, 30 Jul 2005 05:51:13 -0700 } 13, 29 -- Message-ID: } {BAY105-DAV369B71A5CF300715020B4D1C10-at-phx.gbl} } 13, 29 -- Received: from 65.54.224.200 by } BAY105-DAV3.phx.gbl with DAV; } 13, 29 -- Sat, 30 Jul 2005 12:51:13 +0000 } 13, 29 -- X-Originating-IP: [65.54.224.200] } 13, 29 -- X-Originating-Email: [kgmnrc-at-hotmail.com] } 13, 29 -- X-Sender: kgmnrc-at-hotmail.com } 13, 29 -- From: "Keith" {kgmnrc-at-hotmail.com} } 13, 29 -- To: {microscopy-at-microscopy.com} } 13, 29 -- Subject: RE: [Microscopy] Re: [Filtered] } AskAMicroscopist: How to image a snow flake } 13, 29 -- Date: Sat, 30 Jul 2005 08:50:50 -0400 } 13, 29 -- MIME-Version: 1.0 } 13, 29 -- Content-Type: text/plain; } 13, 29 -- charset="us-ascii" } 13, 29 -- Content-Transfer-Encoding: 7bit } 13, 29 -- X-Priority: 1 (Highest) } 13, 29 -- X-MSMail-Priority: High } 13, 29 -- X-Mailer: Microsoft Office Outlook, Build } 11.0.6353 } 13, 29 -- In-Reply-To: } {200507291716.j6THGUF0010120-at-ns.microscopy.com} } 13, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.2180 } 13, 29 -- Importance: High } 13, 29 -- Thread-Index: } AcWUYYq4fLQgcLMPQXeSExZblTjBZQAoTC/A } 13, 29 -- Content-Class: urn:content-classes:message } 13, 29 -- X-OriginalArrivalTime: 30 Jul 2005 } 12:51:13.0605 (UTC) FILETIME=[5E19C750:01C59505] } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 11, 19 -- From hoffpajo-at-yahoo.com Sat Jul 30 19:20:35 2005 11, 19 -- Received: from web50207.mail.yahoo.com (web50207.mail.yahoo.com [206.190.38.48]) 11, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6V0KYTD027442 11, 19 -- for {microscopy-at-microscopy.com} ; Sat, 30 Jul 2005 19:20:34 -0500 11, 19 -- Received: (qmail 23826 invoked by uid 60001); 31 Jul 2005 00:20:34 -0000 11, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 19 -- s=s1024; d=yahoo.com; 11, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 11, 19 -- b=pq3Ilk5emwrCjGiHv8Ylh4xVeMTIYdcDXXmXASBXlZ2vMdjA4XAY5D5pv2dBPvv7UbTOKyVIM1bnyWbhf3qJsNqzXACvdkv0XiIbgRSG9IpyoaBJmP9PCTipmFwH0pyyQ4CN2KqDL1lT3nfW5AftO6+EnYWqfnCh9FIkV81dVxo= ; 11, 19 -- Message-ID: {20050731002034.23824.qmail-at-web50207.mail.yahoo.com} 11, 19 -- Received: from [68.32.53.160] by web50207.mail.yahoo.com via HTTP; Sat, 30 Jul 2005 17:20:34 PDT 11, 19 -- Date: Sat, 30 Jul 2005 17:20:34 -0700 (PDT) 11, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com} 11, 19 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow flake 11, 19 -- To: microscopy-at-microscopy.com 11, 19 -- In-Reply-To: {200507301252.j6UCqQdv025368-at-ns.microscopy.com} 11, 19 -- MIME-Version: 1.0 11, 19 -- Content-Type: text/plain; charset=iso-8859-1 11, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
First, apologies for cross-posting with the Microbeam Analysis Society...
Second, apologies for sending everyone a question with such a narrow focus...
I manage the University of Minnesota's Electron Microprobe Lab, and we have a JEOL JXA-8900R. I am looking to replace our original 1994- vintage HP workstation with something newer and more reliable. I am investigating our options, including just a new HP workstation and switching to the "Probe for Windows" software. I know that my supervisor and the department head will ask if I have checked into every option. In particular, I anticipate they will ask me about the possibility of running the JEOL software on a PC running Linux rather than a HP workstation running HP-UX. Has anyone ever tried this? Would it work? Wouldn't it? I suspect that it wouldn't fully function, but I'd like to be able to speak to the issue with more information than just my guess. Any information or opinions are most welcome.
Thanks, Ellery
-------------------- Ellery E. Frahm Research Scientist/Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu
==============================Original Headers============================== 7, 15 -- From frah0010-at-tc.umn.edu Sat Jul 30 21:21:50 2005 7, 15 -- Received: from mtaout-w.tc.umn.edu (mtaout-w.tc.umn.edu [160.94.160.21]) 7, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6V2LomY003684 7, 15 -- for {Microscopy-at-Microscopy.Com} ; Sat, 30 Jul 2005 21:21:50 -0500 7, 15 -- Received: from [10.0.1.2] (CPE-24-26-167-67.mn.res.rr.com [24.26.167.67]) by mtaout-w.tc.umn.edu with ESMTP for Microscopy-at-Microscopy.Com; Sat, 30 Jul 2005 21:21:50 -0500 (CDT) 7, 15 -- X-Umn-Remote-Mta: [N] CPE-24-26-167-67.mn.res.rr.com [24.26.167.67] #+TS+AU+HN 7, 15 -- Mime-Version: 1.0 (Apple Message framework v733) 7, 15 -- Content-Transfer-Encoding: 7bit 7, 15 -- Message-Id: {962D0566-3096-45CA-88EC-E739056E3A99-at-tc.umn.edu} 7, 15 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 15 -- To: Microscopy-at-Microscopy.Com 7, 15 -- From: Ellery Frahm {frah0010-at-tc.umn.edu} 7, 15 -- Subject: question: JEOL 8900 control 7, 15 -- Date: Sat, 30 Jul 2005 21:21:48 -0500 7, 15 -- X-Mailer: Apple Mail (2.733) ==============================End of - Headers==============================
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