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From: michael shaffer :      michael-at-shaffer.net
Date: Fri, 1 Jul 2005 09:43:19 -0230
Subject: [Microscopy] RE: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John J. Bozzola writes ...


} We are comparing dye sub printers [...]. It is my understanding
} that both produce good quality, gray-scale prints (versus
} dithered, inkjet prints). ...

Inkjet printers may dither, but you cannot see the dithering at all with
modern printers. For excellent color, excellent grayscale, as well as
archival prints, I would suggest you take a look at the 9-ink HP Photosmart
8750. It's only downside is a thirst for ink cartridges, but considering
the prices you are comparing, this printer would be a bargain.

Genuinely, Michael Shaffer :o)
SEM/MLA Lab Coordinator
(709) 737-6790 (Ofc)
(709) 737-6790 (Lab)
{www.micro-investigations.com}
{www.esd.mun.ca/epma/}
Inco Innovation Centre
Memorial University of Newfoundland
St. John's, Newfoundland



From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 07:22:43 2005



From: Alan Stone :      as-at-astonmet.com
Date: Fri, 01 Jul 2005 07:22:01 -0500
Subject: [Microscopy] Re: RE: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Canon i9900. The prints are outstanding and in a way, sadly
better than anything I could do in the darkroom.

Alan Stone
ASTON



At 07:13 AM 7/1/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com



From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 08:08:20 2005



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 1 Jul 2005 08:07:37 -0500
Subject: [Microscopy] RE: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For a few years Pictrography was my printer of choice (average cost per
print
is about $2 or $3). Half a year ago I bought cheap inkjet HP Deskjet
6540
for draft prints. Surprisingly, it produces grayscale prints of very
good quality, so now
I practically stopped using Pictrography.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
} Sent: Thursday, June 30, 2005 4:14 PM
} To: Microscopy-at-msa.microscopy.com
} Subject: [Microscopy] dye sub versus Pictrography
}
}
}
}
} --------------------------------------------------------------
} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} -----------------
}
} We are comparing dye sub printers versus the Fuji Pictrography
} PG4500. The price differential is a staggering $22,000 versus $6,500,
} respectively. It is my understanding that both produce good quality,
} gray-scale prints (versus dithered, inkjet prints). Why the price
} differential? I would welcome any comments, user experiences, etc.
} Does anyone know the average cost per 8.5 x 11in print?
}
} Thank you.
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} ##############################################################
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 08:37:32 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 1 Jul 2005 08:36:50 -0500
Subject: [Microscopy] Re: RE: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have to agree with the ink jet crowd. I have a Canon i9100 for
personal use which will make prints up to 19x13 inches and it also gives
me much more control than I had in the darkroom (and John Bozzola knows
what a switch it is to hear that from me---I used to live in
darkrooms!). I simply couldn't justify paying thousands of dollars for
high-end dye-sub printers or the Fuji system in our lab, when a $500-600
printer will give excellent quality prints with a life span of 100+
years, using the right inks and papers.

I honestly don't even remember when we made the last print for our users
in the lab. I bought a cheaper, but good quality ink-jet a couple of
years ago for people who wanted prints and the original ink cartridges
are still in it. We shoot film on the TEM and scan it and our SEM takes
digital images directly. We give our clients the images on disks and
they're happy. Occasionally we do quick work prints on a laser printer
upon request, but even this is rare.

I still have a darkroom and two enlargers in storage in my garage, but
it's becoming more out of nostalgia than any real hope I'll set them up
again someday. I just can't let go......

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu



-----Original Message-----
} From: Alan Stone [mailto:as-at-astonmet.com]
Sent: Friday, July 01, 2005 7:22 AM
To: microscopy-at-microscopy.com


We have a Canon i9900. The prints are outstanding and in a way, sadly
better than anything I could do in the darkroom.

Alan Stone
ASTON



At 07:13 AM 7/1/2005, you wrote:


} -----------------------------------------------------------------------
} ------- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver

} } both produce good quality, gray-scale prints (versus dithered,
} } inkjet prints). ...
}
} Inkjet printers may dither, but you cannot see the dithering at all
} with modern printers. For excellent color, excellent grayscale, as
} well as archival prints, I would suggest you take a look at the 9-ink
} HP Photosmart 8750. It's only downside is a thirst for ink cartridges,

} but considering the prices you are comparing, this printer would be a
bargain.
}
} Genuinely, Michael Shaffer :o)
} SEM/MLA Lab Coordinator
} (709) 737-6790 (Ofc)
} (709) 737-6790 (Lab)
} {www.micro-investigations.com}
} {www.esd.mun.ca/epma/}
} Inco Innovation Centre
} Memorial University of Newfoundland
} St. John's, Newfoundland

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com





From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 09:02:28 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 01 Jul 2005 10:00:36 -0400
Subject: [Microscopy] Re: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is no reason to spend this kind of money to get excellent B&W
prints. Many fine art photographers are making beautiful prints with ink
jet printers. Check out Lyson.com or inkjetmall.com. Also, continuous
inking systems are available that bypass individual ink cartridges. I
will post more specifics later or early next week.

Geoff


John J. Bozzola wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} We are comparing dye sub printers versus the Fuji Pictrography PG4500.
} The price differential is a staggering $22,000 versus $6,500,
} respectively. It is my understanding that both produce good quality,
} gray-scale prints (versus dithered, inkjet prints). Why the price
} differential? I would welcome any comments, user experiences, etc.
} Does anyone know the average cost per 8.5 x 11in print?
}
} Thank you.



--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 10:53:09 2005



From: Diane Baldwin :      dbaldwin-at-dgisrd.com
Date: Fri, 1 Jul 2005 11:52:28 -0400
Subject: [Microscopy] TEM/SEM Job Accouncement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK (RTP), NORTH
CAROLINA AREA



We have an immediate need for a full-time Electron Microscope Technician
(maintenance and operation). 3+ years TEM and SEM experience in
biological, polymer, carbon/carbon composite, and semi-conductor
electron microscopy required. Send resume and salary requirements to
sjeffers-at-dgisrd.com.




From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 11:15:37 2005



From: Don Chernoff at ASM :      donc-at-asmicro.com
Date: Fri, 1 Jul 2005 11:04:37 -0500
Subject: [Microscopy] Image review: Hard copy vs. PC screen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This post is tangential to the dye sub vs ink jet discussion.
When one needs to compare 2 or more images simultaneously, prints can easily
be laid out on a table, but this is not so easy with on-screen display of
digital images. One thing that we have done in our lab is to install a
second monitor at some workstations and increase the Windows desktop size so
that it spans the two monitors. This is satisfactory for AFM images where
the basic pixel count is 512x512 but may not be so good with other formats.
I am curious to learn what other people do.

When you give your customers digital images only, do you feel there is a
risk they could miss an important comparison?

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]



From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 19:35:59 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Fri, 1 Jul 2005 19:35:18 -0500
Subject: [Microscopy] Re: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don't forget to use RIP software for excellent tonal range. I've seen
photos done with and without and there is a discernible difference and
improvement.
Damian

-----Original Message-----
} From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu]
Sent: Friday, July 01, 2005 9:01 AM
To: John J. Bozzola
Cc: Microscopy-at-msa.microscopy.com

There is no reason to spend this kind of money to get excellent B&W
prints. Many fine art photographers are making beautiful prints with ink
jet printers. Check out Lyson.com or inkjetmall.com. Also, continuous
inking systems are available that bypass individual ink cartridges. I
will post more specifics later or early next week.

Geoff


John J. Bozzola wrote:

} --------------------------------------------------------------------------
----
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
-----
}
}
} We are comparing dye sub printers versus the Fuji Pictrography PG4500.
} The price differential is a staggering $22,000 versus $6,500,
} respectively. It is my understanding that both produce good quality,
} gray-scale prints (versus dithered, inkjet prints). Why the price
} differential? I would welcome any comments, user experiences, etc.
} Does anyone know the average cost per 8.5 x 11in print?
}
} Thank you.



--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************







From MicroscopyL-request-at-ns.microscopy.com Sat Jul 2 02:54:09 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Sat, 2 Jul 2005 00:53:23 -0700
Subject: [Microscopy] Image review: Hard copy vs. PC screen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don;
I tried to purchase an IBM T-220 9 megapixel display, but was
told by IBM that they had killed the product. This was the only display
ever marketed that could display a 2K X 2K camera image showing all of
the pixels on one screen. We still have two screens on our TEM, but need
to either display the images at half resolution, or zoom to display only
part of the image.

John Mardinly

-----Original Message-----
} From: Don Chernoff at ASM [mailto:donc-at-asmicro.com]
Sent: Friday, July 01, 2005 9:05 AM
To: Microscopy List

This post is tangential to the dye sub vs ink jet discussion.
When one needs to compare 2 or more images simultaneously, prints can
easily
be laid out on a table, but this is not so easy with on-screen display
of
digital images. One thing that we have done in our lab is to install a
second monitor at some workstations and increase the Windows desktop
size so
that it spans the two monitors. This is satisfactory for AFM images
where
the basic pixel count is 512x512 but may not be so good with other
formats.
I am curious to learn what other people do.

When you give your customers digital images only, do you feel there is a
risk they could miss an important comparison?

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA &
Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes,
consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]





From MicroscopyL-request-at-ns.microscopy.com Sat Jul 2 03:04:41 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Sat, 2 Jul 2005 01:03:56 -0700
Subject: [Microscopy] dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John;
We have a Fuji Pictrograph 3500, and I have to say that nothing
I have ever seen prints with the resolution and vivid saturation of the
pictrograph, both for color and B&W. However, since all of our
conference rooms got digital projectors, we don't make prints any more.
I used it to make some absolutely beautiful prints of my daughter, but
that's about all. We have had multiple failures of a $1,000 circuit
board, and the Fuji service department is a NIGHTMARE to deal with.
Right now, the printer is inoperative. The most recent batch of paper
and donor sticks to the drum, causing a fault. I don't know if spending
another $400 for new rolls will cure that problem, or if there is
something else wrong with the printer, but we're probably going to just
push it out the door.

John Mardinly
Intel

This is the opinion of the author and not of Intel Corporation.

-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Thursday, June 30, 2005 2:14 PM
To: Microscopy-at-msa.microscopy.com

We are comparing dye sub printers versus the Fuji Pictrography
PG4500. The price differential is a staggering $22,000 versus $6,500,
respectively. It is my understanding that both produce good quality,
gray-scale prints (versus dithered, inkjet prints). Why the price
differential? I would welcome any comments, user experiences, etc.
Does anyone know the average cost per 8.5 x 11in print?

Thank you.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################









From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sun, 3 Jul 2005 11:22:00 -0500
Subject: [Microscopy] Administrivia: Software Testing by Nestor - you may ignore this.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Your may safely ignore this test mesage.

I have updated the security model and the software for the Listserver
this morning , and need to perform a full mailing test to all subscribers
to confirm functionality. Basically I believe that I have patched a few software
holes that spammers have discovered.

I will be monitoring the system for problems the rest of the day.
Hopefully this test will run fine and there will be minimal interruptions.

Should you encounter problems please contact me off-line (zaluzec-at-microscopy.com).


Cheers

Nestor
Your Friendly Neighborhood SysOp

Sunday July 3, 2005 11:20 AM CST
-----------------------------





From: amr2w-at-virginia.edu (by way of Nestor J. Zaluzec)
Date: Sun, 3 Jul 2005 21:03:41 -0500
Subject: [Microscopy] viaWWW: 3-D SEM reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good day all,

Two similar models of this monitor were available: IBM T221 and Viewsonic
VP2290B, both using IBM display panel 22" diagonal, 3840 x 2400 pixels, 204
dpi. Both discontinued. IBM had intentions of replacing T221 with new model
as early as March 2005. I don't know whether they did yet.

We purchased several such monitors, IBM and Viewsonic, for TEM camera
systems. Both models are similar in performance, except Viewsonic is a bit
slower in full resolution mode, which makes no difference for static images.
It's hard for a PC to run 9.2 MP frames at over 20 FPS refresh rate anyway.
In fact, it is better to run 1600 x 1200 desktop most of the time and switch
to 3840 x 2400 for high resolution viewing. 1600 x 1200 keeps fonts and
icons size comfortable, and refresh rate is fast. The display in 3840 x 2400
mode is stunning- like looking through a window on a sunny day. You can take
an eye loupe to the screen and see further detail in the image.

If you will be able to find refurbished or second hand T221 - IBM will honor
original 3 year factory warranty as long as monitor is not physically
damaged. One of our IBM monitors was refurbished, with a screen defect. IBM
replaced the monitor. Consult with IBM regarding the warranty, before buying
used monitor. Have monitor serial number when calling IBM. These monitors
are still available, mostly on e-bay, and through some internet outlets.
Could be even new in box, but not from a regular source.

Is anybody at IBM reading this message? Please comment on a future
availability of equal or better display monitor.

See T221 in EM application at
www-1.ibm.com/industries/healthcare/doc/content/bin/ls_t221_enhancing_electr
on_1.pdf

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
} From: "Mardinly, John" {john.mardinly-at-intel.com}
To: "Don Chernoff at ASM" {donc-at-asmicro.com} ; "Microscopy List"
{microscopy-at-microscopy.com}
Sent: Saturday, July 02, 2005 3:53 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://microscopy.com/MLFormMail.html on Thursday, June 30, 2005 at 12:58:42
---------------------------------------------------------------------------

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: UVa

Title-Subject: [Microscopy] [Filtered] 3-D SEM reconstruction

Question: Hi all,
I know there are software programs that do 3-D images from SEM using stereo pairs, however, what if you have a more complex shaped object? I hear that there has been new software that you can use a series of tilts like TEM tomography to obtain a better reconstruction. Does anybody know about this? Any information would be very much appreciated. Thanks!
-Andrew Roelant

---------------------------------------------------------------------------





From: srandol3-at-utk.edu (by way of MicroscopyListserver)
Date: Sun, 3 Jul 2005 21:04:08 -0500
Subject: [Microscopy] viaWWW: Area analysis scanning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (srandol3-at-utk.edu) from http://www.microscopy.com/MLFormMail.html on Sunday, July 3, 2005 at 19:26:02
---------------------------------------------------------------------------

Email: srandol3-at-utk.edu
Name: Steven Randolph

Organization: university of tennessee

Title-Subject: [Microscopy] [Filtered] MListserver: Area analysis scanning

Question: Hello all,

I have a question that is really more engineering-related than imaging. My question is regarding some of the various scanning modes on Hitachi SEMs such as the S4300, S4700, and S3500N. I need to know some of the specifics about how scanning takes place in area analysis mode and reduced screen mode. In area analysis, is the beam blanked in the region outside the box, or is the pixel size reduced to accomodate the box size? I guess what I'm trying to find out is the dwell time in area analysis and reduced screen mode. I seem to recall that there is a finite settle time associated with scanning in some modes. Anyways, any input you could provide would be much appreciated!

Thanks much,
Steven Randolph

---------------------------------------------------------------------------







From: =?iso-8859-2?B?TC4gS+pwafFza2k=?= :      L.Kepinski-at-int.pan.wroc.pl
Date: Mon, 4 Jul 2005 08:43:08 +0200
Subject: [Microscopy] EDAX PV9800 repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,



We have a Philips SEM 515 equipped with EDX spectrometer. The spectrometer
is EDAX PV 9800 system with UTW detector (freshly repaired) and control unit
with its specialized computer (both hardware and software). The computer is
just dying and most probably can not be repaired. I wonder if anybody tried
to "upgrade" this particular system to PC compatible version. Is it possible
at reasonable cost?

Regards,

Leszek



Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences,
P.O.Box 1410,
50-950 Wroclaw, Poland
e-mail: L.Kepinski-at-int.pan.wroc.pl









From: zaluzec-at-microscopy.com
Date: Mon, 4 Jul 2005 13:54:57 -0500
Subject: [Microscopy] Administrivia: Sorry - another full system test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues:

Well, it was wishful thinking that I could adequitely solve the problem in the
first attempt. After many hours of off-line testing (and too few pints for a holiday
weekend). I need to run yet another full system delivery test. You may notice a
few changes in the configuration of the Email headers in this version.

The previous test basically worked, but it took far too many hours to process & verify
all the addresses. I've taken a whole different aproach with this modification
(after having looked at the results over night).

No need to reply to this message. Again if you have problems posting with this
new version of the filters implements, then please contact me off-line.
(zaluzec-at-microscopy.com)

Nestor
Your Friendly Neighborhood SysOp.

Monday - July 4th, 2005




------------------------------Original Headers------------------------------
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16, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j64Ist7n013713
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16, 11 -- Date: Mon, 4 Jul 2005 13:54:54 -0500
16, 11 -- To: microscopy-at-microscopy.com
16, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
16, 11 -- Subject: Administrivia: Sorry - another full system test
16, 11 -- Content-Type: text/plain; charset="us-ascii"
------------------------------------------------------------------------





From: zaluzec-at-microscopy.com
Date: Mon, 4 Jul 2005 18:44:25 -0500
Subject: [Microscopy] Administrivia: Sorry - another full system test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Testing new X-header 6:45 pm


------------------------------Original Headers------------------------------
1, 11 -- From zaluzec-at-microscopy.com Mon Jul 4 18:44:25 2005
1, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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1, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
1, 11 -- Subject: Testing new X-header 6:45 pm
1, 11 -- Content-Type: text/plain; charset="us-ascii"


------------------------------End of - Headers------------------------------




From: zaluzec-at-microscopy.com
Date: Mon, 4 Jul 2005 18:46:05 -0500
Subject: [Microscopy] Testing new X-header 6:47 pm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Testing new X-header 6:47 pm


------------------------------Original Headers------------------------------
1, 11 -- From zaluzec-at-microscopy.com Mon Jul 4 18:46:04 2005
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1, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
1, 11 -- Subject: Testing new X-header 6:47 pm
1, 11 -- Content-Type: text/plain; charset="us-ascii"


------------------------------End of - Headers------------------------------




From: zaluzec-at-microscopy.com
Date: Mon, 4 Jul 2005 18:48:06 -0500
Subject: [Microscopy] Testing new X-header 6:48 pm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Testing new X-header 6:48 pm


------------------------------Original Headers------------------------------
1, 11 -- From zaluzec-at-microscopy.com Mon Jul 4 18:48:06 2005
1, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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1, 11 -- To: microscopy-at-microscopy.com
1, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
1, 11 -- Subject: Testing new X-header 6:48 pm
1, 11 -- Content-Type: text/plain; charset="us-ascii"


------------------------------End of - Headers------------------------------




From: Stephen.Cody-at-ludwig.edu.au
Date: Mon, 4 Jul 2005 19:10:35 -0500
Subject: [Microscopy] FOM 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The next in the FOM conference series will take place in Perth, (Western) Australia from Sunday April 9th to Wednesday April 12, 2006. Please visit for details www.FocusOnMicroscopy.org

Focus on Microscopy 2006 is the continuation of a successful conference series presenting the latest innovations in optical microscopy and its applications in biology, medicine, material science, and information storage. 3D optical imaging and related theory are important subjects for the conference. The series is as relevant now as at any time in its history as the scientific and engineering communities strive to meet the needs of a surging life sciences sector as well as respond to the sustained pressure on miniaturisation in lithography and data storage.

Also a technical exhibition will be part of the conference. In Jena well over 30 companies participated including the major microscopy companies, see FOM2005 Sponsors & Exhibitors www.focusonmicroscopy.org/2005/sponsors.html .

The 2006 meeting will be held in Perth on Australia's western seaboard. The conference will be held in the nearby port city of Fremantle, at the scenic Esplanade hotel, close to the boat harbour and Perth' famous beaches. Perth's relaxed and outdoor lifestyle should prove an ideal setting for a stimulating and enjoyable meeting - see you there!

Local organising committee:
Stephen Cody
Central Resource for Advanced Microscopy, Ludwig Institute for Cancer Research, Melbourne

Guy Cox
Electron Microscope Unit, University of Sydney

Ewa Goldys
Division of Information and Communication Sciences, Macquarie University, Sydney

Brendan Griffin
Centre for Microscopy and Microanalysis, University of Western Australia, Perth

Miranda Grounds
School of Anatomy and Human Biology, University of Western Australia, Perth

Ian Harper
School of Biomedical Sciences, Monash University, Melbourne

David Jans
Department of Biochemistry and Molecular Biology, Monash University, Melbourne

Min Gu
Centre for Microphotonics, Swinburne University, Melbourne

John Kuo
Centre for Microscopy and Microanalysis, University of Western Australia, Perth

Keith Nugent
School of Physics, University of Melbourne

Paul Rigby
Biomedical Imaging and Analysis Facility, University of Western Australia, Perth

Alpha Yap
Institute for Biomolecular Science, University of Queensland, Brisbane

Conference topics include:
* Confocal and multiphoton-excitation microscopies * Novel illumination and detection strategies - selectiveplane, extended depth of focus, 4pi, structured illumination * Fluorescence - new labels, fluorescent proteins, quantum dots, single molecule, excitation-emission spectroscopy * Time-resolved fluorescence - FRET, FRAP, FLIM, FCS * Coherent non-linear microscopies - SHG, THG, SFG, CARS * Scattering processes: Raman, light scattering spectroscopy, second harmonic * Multi-dimensional imaging * Sub-wavelength resolution - near field microscopy, total internal reflection * Laser manipulation, ablation and microdissection, photoactivation * Magnetic resonance and X-ray microscopy * Image processing and visualisation * Live cell and tissue imaging * Whole tissue imaging - optical coherence tomography, endoscopy, whole animal fluorescence * New tools in genomics, proteomics, phenomics, cytometry * Lithography and data storage

To stay informed on the program, registration and abstract submission for the conference please leave your E-mail address here www.focusonmicroscopy.org/forms/subscr_notification.html .

On behalf of the FocusOnMicroscopy society,
* David Sampson, University of Western Australia
* Fred Brakenhoff, University of Amsterdam, The Netherlands

Stephen H. Cody

Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute For Cancer Research
PO Box 2008 Royal Melbourne Hospital
Parkville  Victoria    3050
Australia
Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
email: stephen.cody-at-ludwig.edu.au
www.ludwig.edu.au/labs/confocal.html
www.ludwig.edu.au/confocal




------------------------------Original Headers------------------------------
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From: baskin-at-bio.umass.edu
Date: Tue, 5 Jul 2005 14:28:09 -0500
Subject: [Microscopy] postdoc open in computer vision for cell motility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Postdoctoral Position: Computer Vision Algorithms for Studying
Biological Growth and Motility

A postdoctoral position is available to join a research
project in the quantification of deformable motion in biology, with
emphasis on growth and cell motility. The position is supported by an
NIH-funded collaboration between Tobias I. Baskin (a biologist at
Umass Amherst) and K. Palaniappan (a computer scientist at University
of Missouri, Columbia). Baskin and Palaniappan have developed new
software for quantifying the spatial distribution of velocity within
a growing plant organ (a root). The software is called RootflowRT and
the biological application is described by van der Weele et al (2003
Plant Physiology, 32:1138-1148). The software implements a novel
algorithm for quantifying deformable motion that combines
structure-tensor and robust-matching approaches. The project is to
enhance and validate RootFlowRT, apply software engineering
principles to the current code base, explore new computational
algorithms, and extend the robust-tensor approach to other kinds of
biological objects, in particular motile animal cells and embryos.
The open position is at Amherst and involves imaging different kinds
of biological object as well as enhancing the software. Applicants
should have experience in some area of image processing, good
programming skills, and, preferably, experience in biology.

Those interested in the position should contact Dr Baskin
(email: baskin-at-bio.umass.edu), and can find further information from
his web page: http://www.bio.umass.edu/biology/baskin/ and the page
for RootflowRT http://meru.rnet.missouri.edu/mvl/bio_motion.

I encourage applications from anyone regardless of skin
color, religion, sex, sexual orientation, or nationality.

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
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5, 14 -- To: microscopy-at-microscopy.com
5, 14 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
5, 14 -- Subject: postdoc open in computer vision for cell motility
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From: zaluzec-at-microscopy.com
Date: Tue, 5 Jul 2005 18:37:28 -0500
Subject: [Microscopy] DiI for morphology measurements?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi, Is anyone aware of a method using DiI for staining cells growing in a
monolayer thickness, targeting the cell membrane, to obtain enough contrast to
yield a binary image of the cells representing their shape/morphology?

I'm growing multi-potent cells in 24-well plates and would like to quantify
their shape (and changes in shape) as they differentiate with time.

I've looked in the literature, and I've found lots of examples of DiI being used
to label neurons in live tissue. However, these papers don't focus so much on
quantitative morphological measurements, but more on what the neurons are
ennervating. I'd like a simple method for cells growing or fixed in a plate or
on a slide, for quantifying shape.

Any suggestions?

Sincerely,
Mike McIntyre

X-from: mamcin-at-umich.edu

==============================Original Headers==============================
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==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Tue Jul 5 18:37:28 2005
9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: MicroscopyListserver {zaluzec-at-microscopy.com}
9, 12 -- Subject: DiI for morphology measurements?
9, 12 -- Content-Type: text/plain; charset="us-ascii"
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From: marie.cantino-at-uconn.edu
Date: Wed, 6 Jul 2005 13:39:00 -0500
Subject: [Microscopy] EM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for companies or individuals providing repair services for
TEMs and SEMs in the New England area. Does anyone know of a
comprehensive list of contacts? Any recommendations?

Any providers or others with relevant information are welcome to
contact me at the phone number listed below, or by e-mail. Thanks.

Marie

Dr. Marie E. Cantino
Director, Electron Microscopy Laboratory
Associate Professor of Physiology and Neurobiology
University of Connecticut Unit 3242
Storrs, CT 06269-3242
Phone: 860-486-3588
Fax: 860-486-6369


==============================Original Headers==============================
5, 20 -- From marie.cantino-at-uconn.edu Wed Jul 6 13:39:00 2005
5, 20 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
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5, 20 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 6 Jul 2005 13:39:00 -0500
5, 20 -- Received: from [137.99.47.197] (d47h197.public.uconn.edu [137.99.47.197])
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5, 20 -- Content-Transfer-Encoding: 7bit
5, 20 -- Reply-To: Cantino Marie {marie.cantino-at-uconn.edu}
5, 20 -- From: Marie Cantino {marie.cantino-at-uconn.edu}
5, 20 -- Subject: EM service
5, 20 -- Date: Wed, 6 Jul 2005 14:44:15 -0400
5, 20 -- To: MSA Listserver {Microscopy-at-msa.microscopy.com}
5, 20 -- X-Mailer: Apple Mail (2.622)
5, 20 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
5, 20 -- X-UConn-MailScanner: Found to be clean
5, 20 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================




From: smalinskas-at-yahoo.com
Date: Wed, 6 Jul 2005 14:11:48 -0500
Subject: [Microscopy] Re: [Microscopy] EM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I can recommend the following:

Ken Converse
Quality Images
Delta PA
(717) 456-5491

He participates in this newsgroup.

Stu Smalinskas
Metallurgist
SKF
Plymouth, Michigan

--- marie.cantino-at-uconn.edu wrote:

}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
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----------------------------------------------------------------------------
}
} I am looking for companies or individuals providing
} repair services for
} TEMs and SEMs in the New England area. Does anyone
} know of a
} comprehensive list of contacts? Any
} recommendations?
}
} Any providers or others with relevant information
} are welcome to
} contact me at the phone number listed below, or by
} e-mail. Thanks.
}
} Marie
}
} Dr. Marie E. Cantino
} Director, Electron Microscopy Laboratory
} Associate Professor of Physiology and Neurobiology
} University of Connecticut Unit 3242
} Storrs, CT 06269-3242
} Phone: 860-486-3588
} Fax: 860-486-6369
}




____________________________________________________
Sell on Yahoo! Auctions – no fees. Bid on great items.
http://auctions.yahoo.com/

==============================Original Headers==============================
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9, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
9, 19 -- Subject: Re: [Microscopy] EM service
9, 19 -- To: marie.cantino-at-uconn.edu, microscopy-at-ns.microscopy.com
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From: GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 6 Jul 2005 14:20:59 -0500
Subject: [Microscopy] Uranyl Acetate Shelf Life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm interested in hearing how long people keep the Uranyl Acetate stain that
they use in grid staining, both the stock and working solution. Currently
we are using a 2% solution in 50% methanol.

How long do you think one could say that they remain fresh after making up?
Presently our lab keeps the stock solution for about a month, but I never
really had any literature to know one way or the other if I could keep it
long.


This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

==============================Original Headers==============================
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5, 19 -- Date: Wed, 6 Jul 2005 14:19:35 -0500
5, 19 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
5, 19 -- Subject: Uranyl Acetate Shelf Life
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From: edelmare-at-muohio.edu
Date: Wed, 6 Jul 2005 16:05:09 -0500
Subject: [Microscopy] Re: [Microscopy] RE: High Res Monitors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Just some thoughts. . .

We've been using multiple LCD monitors lately to increase our
windows desktop size. However, in doing so I have noted that
Matrox does offer a high-resolution graphics card (3840x2400) {
http://www.matrox.com/mga/workstation/3dws/products/special/hr25
6.cfm } and they point to three monitors for this card something
refed to as 9 MP (assume 9 mega pixel ? maybe) monitors
(Viewsonic, IBM T221, and Iiayam)

NVidia Quadro FX series cards also goes to 3840x2400

ATI as a few cards which will deliver 3840 x 2400 (using multiple
monitors)

IBM lists a T221 as their 9.2 megapixel monitor - but I can not find
its avavilablity.

A company called Planar makes a 5-megapixel greyscale monitor.
Dome C5i

www.tridentmicrosystems.co.uk lists a 28.1” 2k x 2k TFT LCD
monitor is designed for traffic management.

NEC lists 2048 x 1536 as their highest resolution monitor. (But
does include 10-bit greyscale as well).

Viewsonic offers a number of "Thin Edge" monitors designed to
used together including stands designed to hold 2,3 or 4 monitors
at once.

There is a company www.9xmedia.com that offers multimonitor
solutions.



(Note: I do not sell montiors, or own stock / interests in any monitor
graphics company. :-)



} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -------------------------------------------------------------------------------
}
} Don;
} I tried to purchase an IBM T-220 9 megapixel display, but was
} told by IBM that they had killed the product. This was the only display
} ever marketed that could display a 2K X 2K camera image showing all of
} the pixels on one screen. We still have two screens on our TEM, but need
} to either display the images at half resolution, or zoom to display only
} part of the image.
}
} John Mardinly
}
} -----Original Message-----
} } From: Don Chernoff at ASM [mailto:donc-at-asmicro.com]
} Sent: Friday, July 01, 2005 9:05 AM
} To: Microscopy List
} Subject: [Microscopy] Image review: Hard copy vs. PC screen
}
}
}
} ------------------------------------------------------------------------
} ------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} http://www.microscopy.com/MicroscopyListserver
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} ------------------------------------------------------------------------
} -------
}
} This post is tangential to the dye sub vs ink jet discussion.
} When one needs to compare 2 or more images simultaneously, prints can
} easily
} be laid out on a table, but this is not so easy with on-screen display
} of
} digital images. One thing that we have done in our lab is to install a
} second monitor at some workstations and increase the Windows desktop
} size so
} that it spans the two monitors. This is satisfactory for AFM images
} where
} the basic pixel count is 512x512 but may not be so good with other
} formats.
} I am curious to learn what other people do.
}
} When you give your customers digital images only, do you feel there is a
} risk they could miss an important comparison?
}
} regards,
} Don Chernoff
} ==================================
} Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
} 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
} INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA &
} Canada)
} web: http://www.asmicro.com Fax: 317-895-5652
} [business activities: analytical services in AFM, AFM probes,
} consulting,
} training,
} calibration and test specimens, calibration and measurement software,
} used NanoScope equipment.]
}
}
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."


==============================Original Headers==============================
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From: uti-at-direcpc.com
Date: Thu, 7 Jul 2005 06:41:13 -0500
Subject: [Microscopy] Ze E10 - entgen ete bad

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear group,

We have an old Zeiss EM10C, which we try to put back to the service.
It come with dual channel Roentgen meter board, MOSFET.
Unfortunately we do not have a circuit diagram, which will allow us to fix
a problem on the board.
Some one has a copy of diagram of that board?

Thanks,
Vlad



==============================Original Headers==============================
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5, 19 -- Date: Thu, 07 Jul 2005 07:41:48 -0400
5, 19 -- From: UTI {uti-at-direcpc.com}
5, 19 -- Subject: Zeiss EM10C - Roentgen meter board
5, 19 -- X-Sender: uti-at-pop3.direcpc.com
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From: jmjenks-at-pacbell.net
Date: Thu, 7 Jul 2005 10:09:30 -0500
Subject: [Microscopy] vaWWW: en EA (Eletn be Anal) entt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(jmjenks-at-pacbell.net) from http://microscopy.com/MLFormMail.html
on Thursday, July 7, 2005 at 09:31:04
---------------------------------------------------------------------------

Email: jmjenks-at-pacbell.net
Name: Jeff Jenks

Organization: micronetpartners.com

Title-Subject: [Filtered] Senior EPMA (Electron Probe Micro Analysis) Scientist position

Question: Senior EPMA (Electron Probe Micro Analysis) Scientist position:

Silicon Valley based semiconductor equipment company seeks Senior EPMA Scientist as follows:

Mission and Duties:

- Actively participate in the development, testing and evaluation of new metrology solutions for semiconductor processing from the concept stage to prototype testing.

- Provide a broad practical and theoretical knowledge base of electron spectroscopy, X-ray optics, and electron probe micro analysis (EPMA) to assess analytical capabilities, application areas and metrology opportunities of various analysis techniques.

- Work with a team of scientists and engineers in system development, prototype testing, and design of experiments.

- Plan, perform and evaluate hardware and application feasibility studies on test setups and complete prototype systems.



Responsibilities:

- Independently perform and evaluate experiments using prototype systems

- Design experiments for EPMA concept feasibility

- Build and modify experimental test systems using hands-on skills

- Provide and maintain up-to-date knowledge of surface characterization techniques

- Model and predict electron and X-ray intensities due to particle-material interaction

- Develop, test, and evaluate measurement protocols

- Actively participate in system development and subsystem designs



Background and Education:

- MS or Ph.D. in material science, physics or related field is required; an emphasis in measurement or instrumentation is preferred

- Broad knowledge in material analysis

- Strong practical experience in electron probe analysis, preferably WDX Wavelength- Dispersive X-ray Spectroscopy or EPMA or equivalent

- Experience in semiconductor material analysis is preferred but not required

- Intimate knowledge of data reduction methods, statistical analysis and principles of particle-material interaction

- Knowledge of X-ray optics and/or X-ray optical design is desirable

- Ability to work independently and within a team of scientists and engineers

- Minimum of 2 years of practical experience in a combination of the above areas



Company offers competitive salary, stock options, benefits, and relocation assistance in a collegial work environment. Company is privately held and VC-funded with outstanding prospects for stock price appreciation in the future.



For immediate consideration please contact Jeff Jenks with your resume or CV at Jeff-at-micronetpartners.com. Or you can call Jeff at 408-850-7271 for additional details. No visa sponsorship is available. For additional openings please view www.micronetpartners.com/jobs.html.


---------------------------------------------------------------------------

==============================Original Headers==============================
40, 13 -- From zaluzec-at-microscopy.com Thu Jul 7 10:09:30 2005
40, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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40, 13 -- To: microscopy-at-microscopy.com
40, 13 -- From: jmjenks-at-pacbell.net (by way of MicroscopyListserver)
40, 13 -- Subject: viaWWW: Senior EPMA (Electron Probe Micro Analysis) Scientist
40, 13 -- position
40, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 7 Jul 2005 10:45:32 -0500
Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Sorry, I seem to have scrambled the subject line of the last few postings
with my latest version of the filter code. Please do not critique the
person posting it was my fault.


Nestor
Your Friendly Neighborhood SysOp

--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
NEESLab: http://neestpm.mcs.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================

==============================Original Headers==============================
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7, 14 -- To: microscopy-at-microscopy.com
7, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
7, 14 -- Subject: Administrivia: Subject Line scrambled...sorry
7, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 7 Jul 2005 10:48:26 -0500
Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Colleagues

Sorry, I seem to have scrambled the subject line of the last few postings
with my latest version of the filter code. Please do not critique the
person posting it was my fault.


Nestor
Your Friendly Neighborhood SysOp




--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
NEESLab: http://neestpm.mcs.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================

==============================Original Headers==============================
13, 14 -- From zaluzec-at-aaem.amc.anl.gov Thu Jul 7 10:48:26 2005
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13, 14 -- To: microscopy-at-microscopy.com
13, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
13, 14 -- Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry
13, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jmjenks-at-pacbell.net
Date: Thu, 7 Jul 2005 10:48:44 -0500
Subject: [Microscopy] viaWWW: Senior EPMA (Electron Probe Micro Analysis) Scientist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(jmjenks-at-pacbell.net) from http://microscopy.com/MLFormMail.html
on Thursday, July 7, 2005 at 09:31:04
---------------------------------------------------------------------------

Email: jmjenks-at-pacbell.net
Name: Jeff Jenks

Organization: micronetpartners.com

Title-Subject: [Filtered] Senior EPMA (Electron Probe Micro Analysis) Scientist position

Question: Senior EPMA (Electron Probe Micro Analysis) Scientist position:

Silicon Valley based semiconductor equipment company seeks Senior EPMA Scientist as follows:

Mission and Duties:

- Actively participate in the development, testing and evaluation of new metrology solutions for semiconductor processing from the concept stage to prototype testing.

- Provide a broad practical and theoretical knowledge base of electron spectroscopy, X-ray optics, and electron probe micro analysis (EPMA) to assess analytical capabilities, application areas and metrology opportunities of various analysis techniques.

- Work with a team of scientists and engineers in system development, prototype testing, and design of experiments.

- Plan, perform and evaluate hardware and application feasibility studies on test setups and complete prototype systems.



Responsibilities:

- Independently perform and evaluate experiments using prototype systems

- Design experiments for EPMA concept feasibility

- Build and modify experimental test systems using hands-on skills

- Provide and maintain up-to-date knowledge of surface characterization techniques

- Model and predict electron and X-ray intensities due to particle-material interaction

- Develop, test, and evaluate measurement protocols

- Actively participate in system development and subsystem designs



Background and Education:

- MS or Ph.D. in material science, physics or related field is required; an emphasis in measurement or instrumentation is preferred

- Broad knowledge in material analysis

- Strong practical experience in electron probe analysis, preferably WDX Wavelength- Dispersive X-ray Spectroscopy or EPMA or equivalent

- Experience in semiconductor material analysis is preferred but not required

- Intimate knowledge of data reduction methods, statistical analysis and principles of particle-material interaction

- Knowledge of X-ray optics and/or X-ray optical design is desirable

- Ability to work independently and within a team of scientists and engineers

- Minimum of 2 years of practical experience in a combination of the above areas



Company offers competitive salary, stock options, benefits, and relocation assistance in a collegial work environment. Company is privately held and VC-funded with outstanding prospects for stock price appreciation in the future.



For immediate consideration please contact Jeff Jenks with your resume or CV at Jeff-at-micronetpartners.com. Or you can call Jeff at 408-850-7271 for additional details. No visa sponsorship is available. For additional openings please view www.micronetpartners.com/jobs.html.


---------------------------------------------------------------------------

==============================Original Headers==============================
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40, 13 -- To: microscopy-at-microscopy.com
40, 13 -- From: jmjenks-at-pacbell.net (by way of MicroscopyListserver)
40, 13 -- Subject: viaWWW: Senior EPMA (Electron Probe Micro Analysis) Scientist
40, 13 -- position
40, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 7 Jul 2005 10:51:16 -0500
Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

Sorry, I seem to have scrambled the subject line of the last few postings
with my latest version of the filter code. Please do not critique the
person posting it was my fault.

I have reposted at least one of the scrambled messages.


Nestor
Your Friendly Neighborhood SysOp



==============================Original Headers==============================
8, 14 -- From zaluzec-at-aaem.amc.anl.gov Thu Jul 7 10:51:16 2005
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8, 14 -- Date: Thu, 7 Jul 2005 10:51:13 -0500
8, 14 -- To: microscopy-at-microscopy.com
8, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
8, 14 -- Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry
8, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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From: cpetty1-at-umbc.edu
Date: Thu, 7 Jul 2005 11:58:28 -0500
Subject: [Microscopy] TEM local service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings from Baltimore. I have a Zeiss 10 CA. Our past service company
Pesto Inc. longer is in business. Does anyone know of a person or
company that works on Zeiss TEMs in my area?
--
Chere Petty, M.S.
Manager, Keith R. Porter Imaging Facility
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
1000 Hilltop Circle
Baltimore, MD 21250
Phone: 410-455-2296
Fax: 410-455-3875

==============================Original Headers==============================
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1, 21 -- From: Chere Petty {cpetty1-at-umbc.edu}
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1, 21 -- X-Avmilter: Message Skipped, too small
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From: uti-at-direcpc.com
Date: Thu, 7 Jul 2005 12:06:12 -0500
Subject: [Microscopy] Zeiss EM10C - Roentgen meter board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dear group,

We have an old Zeiss EM10C, which we try to put back to the service.
It come with dual channel Roentgen meter board, MOSFET.
Unfortunately we do not have a circuit diagram, which will allow us to fix
a problem on the board.
Some one has a copy of diagram of that board?

Thanks,
Vlad




==============================Original Headers==============================
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From: vincent.metzger-at-philips.com
Date: Thu, 7 Jul 2005 13:01:05 -0500
Subject: [Microscopy] viaWWW: Inca energy - real K-ratio ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(vincent.metzger-at-philips.com) from http://www.microscopy.com/MLFormMail.html
on Thursday, July 7, 2005 at 11:26:23
---------------------------------------------------------------------------

Email: vincent.metzger-at-philips.com
Name: Vincent Metzger

Organization: philips

Title-Subject: [Filtered] Inca energy - real K-ratio ?

Question: I'm looking for thickness measurement on a stratified sample by using HT variation on SEM and a software to modelize theoricaly the interactions in the stratified sample.
I need to extract real Kratio.
I made some tests by passing a standart of silicium (for instance), then my sample (containig a strat of Si) and making the ratio between the 2 intensity. This work quite well, but rather time consuming.
I standardized my silicium in the inca energy software, but the K-ratio displayed was totally wrong.
As an example the system uses Co for quant optimization, and when you analyse the Co, a 1.6 ratio is found:crazy.

I'm wondering how the soft works, very ergonomic but rather a black box.
What coefficient is applied? How to get rid of?
Is there a option that can be disabled or something else?



---------------------------------------------------------------------------

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From: yaseki-at-ucsd.edu
Date: Thu, 7 Jul 2005 19:31:36 -0500
Subject: [Microscopy] =?iso-8859-1?Q?AskAMicroscopist=8A?= help for LEO 438VP SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55).
It was submitted by (yaseki-at-ucsd.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Thursday, July 7, 2005 at 18:25:53
---------------------------------------------------------------------------

Email: yaseki-at-ucsd.edu
Name: Seki Yasuaki

Organization: University of Calfornia-San Diego

Education: Graduate College

Location: La Jolla, CA, USA

Question: Looking for vacuum schemtics, connection help for LEO 438VP SEM. The disassembled instrument has an Edwards Turbo backed by Edwards RV12 pump. The isolation block has an additional port (NW25 fitting) for connection to the specimen chamber. The microscope also has a solenoid operated PV25EK valve. I am looking for info on where this valve is to be located? a) between the block and turbo? or b) between the block and the specimen chamber? or c) elsewher? Any pictures would be of immense help as well.

---------------------------------------------------------------------------

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From: benada-at-biomed.cas.cz
Date: Fri, 8 Jul 2005 04:23:12 -0500
Subject: [Microscopy] Carbon evaporation troubles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good day all,

My apologies if this posting a repeater- I had problems with my e-mail in
the past week.

Two similar models of this monitor were available: IBM T221 and Viewsonic
VP2290B. Both (I was told) used IBM display panel 22" diagonal, 3840 x 2400
pixels, 204
dpi. Both discontinued. I was also told that IBM had plans of replacing T221
with a
new model as early as March 2005. I don't know whether they did yet.

We purchased several such monitors, IBM and Viewsonic, for TEM camera
systems. Both models are similar in performance, except Viewsonic is a bit
slower in full resolution mode, which makes no difference for static images.
It's hard for a PC to run 9.2 MP frames at over 20 FPS refresh rate anyway.
In fact, it is better to run 1600 x 1200 desktop most of the time and switch
to 3840 x 2400 for high resolution viewing. 1600 x 1200 keeps fonts and
icons size comfortable, and refresh rate is fast. The display in 3840 x 2400
mode is stunning- like looking through a window on a sunny day. You can take
an eye loupe to the screen and see further detail in the image.

If you will be able to find refurbished or second hand T221 - IBM will honor
original 3 year factory warranty as long as monitor is not physically
damaged. One of our IBM monitors was refurbished, with a screen defect. IBM
replaced the monitor. Consult with IBM regarding the warranty, before buying
used monitor. Have monitor serial number when calling IBM. These monitors
are still available, mostly on e-bay, and through some internet outlets.
Could be even new in box, but not from a regular source.

Is anybody at IBM reading this message? Please comment on a future
availability of equal or better display monitor.

See IBM paper on T221 in EM application at
http://www-1.ibm.com/industries/healthcare/doc/content/bin/ls_t221_enhancing_electron_1.pdf

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
X-from: "Mardinly, John" {john.mardinly-at-intel.com}
To: "Don Chernoff at ASM" {donc-at-asmicro.com} ; "Microscopy List"
{microscopy-at-microscopy.com}
Sent: Saturday, July 02, 2005 3:53 AM

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

Hi,

We have troubles with evaporation control unit BSV 202 of our BALZERS BAF
301 FE device. Does anybody have some experiences with it?

The current needed for carbon evaporation suddenly jumped so high that the
8 A fuse blown out happend. After replacing it, the current needed for
carbon evaporation stays too high and exceeds the permitted current of
safeguarding 8A fuse. There are no differences if we use evaporator 1
(transformer 1) or evaporator 2 (transformer 2).
For carbon evaporation we use sharpenned carbon rods.

Thanking in advance for any suggestion.
Oldrich
-------------------------------------------------------------------------
Oldrich Benada
Institute of Microbiology, Acad. Sci. CR
Lab. EM
Videnska 1083
142 20 Prague 4
Czech Republic


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From: mozaluzec-at-microscopy.com
Date: Fri, 8 Jul 2005 07:49:42 -0500
Subject: [Microscopy] Solid Mortgages for Americans

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

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This offer is being extended to you unconditionally and your credit is in no way a factor.

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Ciao,

Pierson Louann

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From: zaluzec-at-microscopy.com
Date: Fri, 8 Jul 2005 09:32:44 -0500
Subject: [Microscopy] Admin Test Message 9:32 AM

Contents Retrieved from Microscopy Listserver Archives
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Admin Test Message 9:32 AM
Admin Test Message 9:32 AM

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From: edelmare-at-muohio.edu
Date: Fri, 8 Jul 2005 09:52:35 -0500
Subject: [Microscopy] Imaging thin films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

O.k., I'm a materials neophyte and I'm looking for some
direction. I can ultramicrotome things, I can crush and fracture
things, and I can physically polish things, but now I am facing a new
sample area: How do I prepare thin films, inorganic, crystal and
semi-crystaline grown on a hard smooth substraight (glass, Si,
Mica, etc.)? Film thickness below 1-micrometer, mostly below
300nm. We would like to study the morphology of the film via SEM
or TEM. EDS and EBSD would also be nice. So how do I get a
clean cross-section of the thin film? Buying a dual-column/beam
FIB/SEM might be a nice idea but seems a little over-kill, eh? Can
someone point me in the right direction? Ion-beam milling? Ion-
beam polishing? Sectioning glass or Si crystal just does not sound
like a good idea.

Next are there any thoughts on buying equipment and doing this in
house vs having samples preped off-campus (I acknowledge that
there are some techniques and equipment that are just really tricky).

Thank you!



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

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From: Mike.Bode-at-soft-imaging.net
Date: Fri, 8 Jul 2005 10:08:39 -0500
Subject: [Microscopy] For Nestor: Double posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

Hi Nestor,

As of today I am getting all postings twice. Is that a problem with my
subscription or a general problem?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================



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From: gary-at-gaugler.com
Date: Fri, 8 Jul 2005 10:24:45 -0500
Subject: [Microscopy] Re: Imaging thin films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

SEM will produce morphology info whereas EBSD will produce
grain, orientation and texture info. Totally different
results and prep methods.

Depending on what the film material is, that would dictate
how it is prepared for analysis. Silicon is easiest I think
to use as a substrate since it can easily be cut into small
pieces. Mount the piece and coat it then SEM image for
morphology. Mount another piece and polish it with colloidal
silica or alumina (again, depending on film material) down
to .02u and this should work for EBSD.

FIB ablades the surface and does not produce good EBSD specimens.
I've tried various plasmas and Gatan 682 and do not get good specimens
for EBSD. There is probably some magic combination that I have
yet to find. Thus far, mechanical polishing and slight etch
with DI water seems to do the job. There are dedicated ion beam
polishing tools that do nice jobs for EBSD. But unless you are
going to do a lot of this, I don't think it pencils out.

Since the analysis is tops down, I don't see why you would
need to section the specimen. If there is more to your
situation, tell us more.

gary g.


At 07:55 AM 7/8/2005, you wrote:

} O.k., I'm a materials neophyte and I'm looking for some
} direction. I can ultramicrotome things, I can crush and fracture
} things, and I can physically polish things, but now I am facing a new
} sample area: How do I prepare thin films, inorganic, crystal and
} semi-crystaline grown on a hard smooth substraight (glass, Si,
} Mica, etc.)? Film thickness below 1-micrometer, mostly below
} 300nm. We would like to study the morphology of the film via SEM
} or TEM. EDS and EBSD would also be nice. So how do I get a
} clean cross-section of the thin film? Buying a dual-column/beam
} FIB/SEM might be a nice idea but seems a little over-kill, eh? Can
} someone point me in the right direction? Ion-beam milling? Ion-
} beam polishing? Sectioning glass or Si crystal just does not sound
} like a good idea.
}
} Next are there any thoughts on buying equipment and doing this in
} house vs having samples preped off-campus (I acknowledge that
} there are some techniques and equipment that are just really tricky).
}
} Thank you!
}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu


==============================Original Headers==============================
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From: biology-at-ucla.edu
Date: Fri, 8 Jul 2005 10:49:40 -0500
Subject: [Microscopy] via-WWW: Looking for Judy Murphy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (biology-at-ucla.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, July 8, 2005 at 10:41:07
---------------------------------------------------------------------------

Email: biology-at-ucla.edu
Name: Eric A. Rosen

Organization: UCLA Medical Center

Title-Subject: I am trying to get a hold of Judy Murphy at the San Joaquin Delta

Question: I am trying to get a hold of Judy Murphy at the San Joaquin Delta College EM School
I have a open EM position and would like to advertise it with her...


Thanks

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Fri Jul 8 10:49:40 2005
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8, 12 -- Subject: via-WWW: Looking for Judy Murphy
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==============================End of - Headers==============================




From: phillipst-at-missouri.edu
Date: Fri, 8 Jul 2005 13:17:07 -0500
Subject: [Microscopy] paraffin microtomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello John,

I am not quite sure what it means when you say that the resolution of the
human eye is 300 DPI. I looked up an article on the web
(http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which
specifies the angular resolution of the human eye under optimum conditions
as 1/60th of a degree. If I do my math right, the resolution is then given
by:

2 x distance x tan (angle/2).

For a viewing distance of about 1m, I get a resolution of 0.3mm (which
corresponds to about 220 DPI at 1m distance), which is more or less the dot
pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of
0.294mm). So, I think the normal monitors are actually at the limits of the
human eye's resolution. If your eyes were much better, you should be able to
see the individual red, green, and blue dots from a distance of 1m.

As I said, the optimum resolution assumes certain parameters: 25 cm viewing
distance, perfect lighting, etc. All changes will reduce the resolution.

But that's all mathematics. Has anybody actually compared a high res monitor
and a regular monitor side by side?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 10:04 AM
To: Mike Bode

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

I am considering buying a paraffin microtome for our core facility. I don't
personally use one much so if any knowledgeable users have recommendations
on brands or models to buy (or avoid), i would welcome replies (probably
best sent to me directly and not the listserver). I will be happy to keep
your comments confidential. In addition, if there are special features you
think are essential, i would be pleased to hear of them also. thanks, tom



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



==============================Original Headers==============================
7, 18 -- From PhillipsT-at-missouri.edu Fri Jul 8 13:17:07 2005
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7, 18 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 8 Jul 2005 13:17:07 -0500
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7, 18 -- Date: Fri, 08 Jul 2005 13:17:02 -0500
7, 18 -- To: Microscopy-at-msa.microscopy.com
7, 18 -- From: Tom Phillips {phillipst-at-missouri.edu}
7, 18 -- Subject: paraffin microtomes
7, 18 -- Mime-Version: 1.0
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7, 18 -- X-OriginalArrivalTime: 08 Jul 2005 18:17:05.0890 (UTC) FILETIME=[3F153420:01C583E9]
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Fri, 8 Jul 2005 16:04:46 -0500
Subject: [Microscopy] Re: ink jet versus dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike;
One meter viewing distance? Sorry, my arms are no where near that long. Fortunately my presbyopia is compensated by severe myopia. Otherwise, use reading glasses. Closer up (~1 foot?), the human eye can resolve ~80-100 microns, which is 250-300 DPI, which is why probably why 300 DPI has been a target spec for printers for a long time. The IBM T221 has a pixel pitch of 0.1245 millimeters, so those pixels are half the size of the pixels of garden variety monitors that cost less, and the way that translates into 200DPI is (25 mm/in)/(0.1245 mm/pixel)=200.8 pixels/inch. BTW, I can see the vertical mask stripes of my Sony monitor with my naked eye if I get close, and everybody in our lab could see the difference between a 400DPI Fuji Pictrograph print and a monitor display.

John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Mike.Bode-at-soft-imaging.net [mailto:Mike.Bode-at-soft-imaging.net]
Sent: Friday, July 08, 2005 9:46 AM
To: Mardinly, John

Hello John,

I am not quite sure what it means when you say that the resolution of the
human eye is 300 DPI. I looked up an article on the web
(http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which
specifies the angular resolution of the human eye under optimum conditions
as 1/60th of a degree. If I do my math right, the resolution is then given
by:

2 x distance x tan (angle/2).

For a viewing distance of about 1m, I get a resolution of 0.3mm (which
corresponds to about 220 DPI at 1m distance), which is more or less the dot
pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of
0.294mm). So, I think the normal monitors are actually at the limits of the
human eye's resolution. If your eyes were much better, you should be able to
see the individual red, green, and blue dots from a distance of 1m.

As I said, the optimum resolution assumes certain parameters: 25 cm viewing
distance, perfect lighting, etc. All changes will reduce the resolution.

But that's all mathematics. Has anybody actually compared a high res monitor
and a regular monitor side by side?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 10:04 AM
To: Mike Bode

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

Hi John,

I was actually talking about monitors. I agree with you that you normally
look at prints at a shorter distance, but I think 1 m for a monitor is a
reasonable distance.

And what you are saying ("BTW, I can see the vertical mask stripes of my
Sony monitor with my naked eye if I get close") basically confirms my
assumption: If you are NOT up close, you can't see the stripe, so the pixel
size is close to resolution of the eye (at normal viewing distances). You
would not be able to see or detect a further reduction in pixel size. I
don't know if the width of the vertical mask is the same as a pixel. I, for
one, cannot see individual green, red and blue pixels when I look at a white
spot on the monitor, unless I use a magnifying glass.

Comparing printers and monitors is difficult. One is "back-illuminated", the
other shows reflected light, the color gamut is different, etc. I'd be
interested to hear from someone who has compared a normal LCD monitor with a
high resolution LCD monitor.

mike



Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:11 PM
To: Mike Bode
Cc: Listserver

Hello John,

I am not quite sure what it means when you say that the resolution of the
human eye is 300 DPI. I looked up an article on the web
(http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which
specifies the angular resolution of the human eye under optimum conditions
as 1/60th of a degree. If I do my math right, the resolution is then given
by:

2 x distance x tan (angle/2).

For a viewing distance of about 1m, I get a resolution of 0.3mm (which
corresponds to about 220 DPI at 1m distance), which is more or less the dot
pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of
0.294mm). So, I think the normal monitors are actually at the limits of the
human eye's resolution. If your eyes were much better, you should be able to
see the individual red, green, and blue dots from a distance of 1m.

As I said, the optimum resolution assumes certain parameters: 25 cm viewing
distance, perfect lighting, etc. All changes will reduce the resolution.

But that's all mathematics. Has anybody actually compared a high res monitor
and a regular monitor side by side?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 10:04 AM
To: Mike Bode

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

Mike;
Phil Batson reported at M&M 2004 that it was extremely useful and highly recommended. Vitaly Feingold reported this week in the Listserver that he has two. Perhaps he can also describe the experience of beholding an IBM T221.

John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Mike.Bode-at-soft-imaging.net [mailto:Mike.Bode-at-soft-imaging.net]
Sent: Friday, July 08, 2005 12:49 PM
To: Mardinly, John

Hi John,

I was actually talking about monitors. I agree with you that you normally
look at prints at a shorter distance, but I think 1 m for a monitor is a
reasonable distance.

And what you are saying ("BTW, I can see the vertical mask stripes of my
Sony monitor with my naked eye if I get close") basically confirms my
assumption: If you are NOT up close, you can't see the stripe, so the pixel
size is close to resolution of the eye (at normal viewing distances). You
would not be able to see or detect a further reduction in pixel size. I
don't know if the width of the vertical mask is the same as a pixel. I, for
one, cannot see individual green, red and blue pixels when I look at a white
spot on the monitor, unless I use a magnifying glass.

Comparing printers and monitors is difficult. One is "back-illuminated", the
other shows reflected light, the color gamut is different, etc. I'd be
interested to hear from someone who has compared a normal LCD monitor with a
high resolution LCD monitor.

mike



Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:11 PM
To: Mike Bode
Cc: Listserver

Hello John,

I am not quite sure what it means when you say that the resolution of the
human eye is 300 DPI. I looked up an article on the web
(http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which
specifies the angular resolution of the human eye under optimum conditions
as 1/60th of a degree. If I do my math right, the resolution is then given
by:

2 x distance x tan (angle/2).

For a viewing distance of about 1m, I get a resolution of 0.3mm (which
corresponds to about 220 DPI at 1m distance), which is more or less the dot
pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of
0.294mm). So, I think the normal monitors are actually at the limits of the
human eye's resolution. If your eyes were much better, you should be able to
see the individual red, green, and blue dots from a distance of 1m.

As I said, the optimum resolution assumes certain parameters: 25 cm viewing
distance, perfect lighting, etc. All changes will reduce the resolution.

But that's all mathematics. Has anybody actually compared a high res monitor
and a regular monitor side by side?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 10:04 AM
To: Mike Bode

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

John et al.:

A few more details on what I wrote on this subject before. Lyson has
been making inks and papers for photo quality ink jet printing for some
time. Their new system is the "Daylight Darkroom". It is a dedicated 4
to 7 ink system (depending on your printer) for black and white printing
only. I think the price includes software and inks but no printer, the
system only works with certain printers. It has gotten very favorable
reviews in fine art photo magazines (May/June 2005 issue of Photo
Techniques, the review might be on the magazine's website
(www.phototechmag.com) or on Lyson's). Lyson will send you some sample
prints if you register at their website. I suspect that if someone sent
Lyson a digital TEM or SEM image they might make a print of that as a
sales tool. I'll bet they have no idea that there is a market for their
products in the EM field. I only wish I did more than a few prints per
year of parts of ground up cells.
Lyson is not the only firm making inks, papers and printing systems
for fine art B&W photography. Check out InkjetMall.com or MediaStreet.com.
Disclaimer: I don't have any financial interest in any of the firms
or products I mentioned.

Geoff


John J. Bozzola wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} We are comparing dye sub printers versus the Fuji Pictrography PG4500.
} The price differential is a staggering $22,000 versus $6,500,
} respectively. It is my understanding that both produce good quality,
} gray-scale prints (versus dithered, inkjet prints). Why the price
} differential? I would welcome any comments, user experiences, etc.
} Does anyone know the average cost per 8.5 x 11in print?
}
} Thank you.

--

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: cgarber-at-2spi.com
Date: Fri, 8 Jul 2005 17:02:15 -0500
Subject: [Microscopy] Characterization of thin film coatings by TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard E. Edelmann wrote:
===========================================
O.k., I'm a materials neophyte and I'm looking for some
direction. I can ultramicrotome things, I can crush and fracture
things, and I can physically polish things, but now I am facing a new
sample area: How do I prepare thin films, inorganic, crystal and
semi-crystaline grown on a hard smooth substraight (glass, Si,
Mica, etc.)? Film thickness below 1-micrometer, mostly below
300nm. We would like to study the morphology of the film via SEM
or TEM. EDS and EBSD would also be nice. So how do I get a
clean cross-section of the thin film? Buying a dual-column/beam
FIB/SEM might be a nice idea but seems a little over-kill, eh? Can
someone point me in the right direction? Ion-beam milling? Ion-
beam polishing? Sectioning glass or Si crystal just does not sound
like a good idea.

Next are there any thoughts on buying equipment and doing this in
house vs having samples preped off-campus (I acknowledge that
there are some techniques and equipment that are just really tricky).
===============================================
Since you are already set up to do ultramicrotomy, have you considered the following:

a) Take a freshly cleaved stripping of HOPG and then do your evaporation. If you are
not familiar with HOPG, see URL
http://www.2spi.com/catalog/new/hopgsub.shtml In terms of smoothness, HOPG can be
atomically smooth, the size of the atomically smooth areas depending on the grade of
the HOPG selected.

b) Put the HOPG stripping in a flat embedding mold and then expose the coated
sample, HOPG side up, to an oxygen plasma, such as in one of our Plasma Prep II
plasma etchers, which will etch away the HOPG but leave your coating intact.

c) Fill the cavity containing the thin film, now with HOPG removed, with your
favorite Epon 812 substitute, or SPI-Pon 812 and after curing,

d) Face off the block and diamond knife thin section the coating.

A variation on this theme would be to substitute a freshly cleaved surface of NaCl
for the substrate, and after coating, the NaCl is dissolved away and the coating
when dry, can be embedded as above.

You should have no difficulty getting good TEM images of the coating.

We actually prefer diamond knife thin sectioning to other methods for this kind of
sample. It is not a method free of artifacts. But when they are ones that are
induced by the knife edge itself, they are directional and easily recognized as
being artifacts, but for the other approaches one might consider, the artifacts are
not directional and therefore much more difficult to recognize.

Disclaimer: SPI Supplies offers HOPG, plasma etchers, diamond knives, and NaCl
crystals so we would have a vested interest in promoting this approach relative to
others.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================


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From: cgarber-at-2spi.com
Date: Sat, 9 Jul 2005 03:09:02 -0500
Subject: [Microscopy] carbon evaporation problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oldrich Benada wrote:
=================================================================
We have troubles with evaporation control unit BSV 202 of our BALZERS BAF
301 FE device. Does anybody have some experiences with it?

The current needed for carbon evaporation suddenly jumped so high that the
8 A fuse blown out happend. After replacing it, the current needed for
carbon evaporation stays too high and exceeds the permitted current of
safeguarding 8A fuse. There are no differences if we use evaporator 1
(transformer 1) or evaporator 2 (transformer 2).
For carbon evaporation we use sharpenned carbon rods.

Thanking in advance for any suggestion.
=================================================================
Have you switched sources for your "carbon" rods lately? First, some
vendors offering "carbon" rods are really offering graphite rods. It is my
own perception that most people who think they are using carbon rods are
actually using graphite rods. Also, even among graphite rods, there is a
large variation of resistivities (due to variations in density). So a
common problem is that one procures the wrong kind of rods (which would have
a resistivity that is out of range for their instrumental set up) so that in
order to get evaporation, they exceed the instrumental parameters of their
system. See URL
http://2spi.com/catalog/spec_prep/carbon-graphite-rods.html
for our simple "test" to judge which type of rod you are actually using.

If you have not switched rod vendors, then the second most common reason is
that you are not making a sharp enough "point". Hence the tip, in order to
get hot enough to evaporate carbon, draws so much current, you exceed the
limits of your system. For the optimum dimensions for rod tips, see the
drawings on URL
http://2spi.com/catalog/spec_prep/carbon-rods.shtml

Rods can be purchased pre-sharpened from all of the major suppliers of
consumables for EM laboratories, such as SPI, but if you want to make your
own presharpened points, carbon rod sharpeners can be purchased as well such
as are found on URL
http://2spi.com/catalog/spec_prep/carbon-rod-sharpener.shtml

Disclaimer: SPI Supplies offers carbon and graphite rods as well as
sharpeners.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



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From: Mike.Bode-at-soft-imaging.net
Date: Sat, 9 Jul 2005 11:48:17 -0500
Subject: Re: [Microscopy] RE: RE: RE: RE: RE: Image review: Hard copy vs. PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It is unquestionably right that the distance of best resolution for the
human eye is about 25 cm. However, I was talking about working on a monitor,
and I am typically sitting at arm's length from it. I just measured my arm,
and it is 75 cm. I do not work with a monitor with my eyes 25 cm from the
screen. Would definitely give me headaches.

I am not trying to prove to everyone that high res monitors are useless, far
from it. But I am personally not convinced that it would be worth $10,000.
Considering the eye's resolution and the options of today's software, I
think you can easily get what you want on a lower res monitor.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 18:50
To: Mike Bode

Barbara;
YES! 10 inches is the right number! That's just slightly less than
the length of my arms, and slightly more than the length of my nose, so
everything I look at ends up at about 10 inches.

John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Barbara Foster [mailto:bfoster-at-mme1.com]
Sent: Friday, July 08, 2005 2:59 PM
To: Mardinly, John




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From: zaluzec-at-microscopy.com
Date: Sat, 9 Jul 2005 12:04:43 -0500
Subject: [Microscopy] Distance to the Monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike etal

For someone that also has far too much monitor/eye neck strain
I also work at 75 cm distance. To make matters worse my
desktop systems are both 3200x1200 pixels... (Dual HR Monitors)
and I sometimes run with multiple virtual desktops
(giving me an effective desktop area of 6400x3200 pixels. You can't
display this all at once, but switching is very quick (on a Mac or Linux box)

I guess this is just a comment on technology. We are now overloaded
sometimes with far too much information or we are just "parallell processing"
alot now adays. Thinking back I don't see how I used to get along with the
old 640x480 monitors or even worse the old VT100 text terminals.

Sigh....

Nestor
Your Friendly Neighborhood SysOp

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From: normzoo-at-yahoo.com
Date: Sun, 10 Jul 2005 10:24:37 -0500
Subject: [Microscopy] viaWWW: LM Nikon AFM microscope camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (normzoo-at-yahoo.com) from http://microscopy.com/MLFormMail.html on Sunday, July 10, 2005 at 04:46:26
---------------------------------------------------------------------------

Email: normzoo-at-yahoo.com
Name: Norman Shedlo

Title-Subject: [Filtered] MListserver: LM Nikon AFM microscope camera

Question: This piece of equipment seems to be relatively common. What is the usual reason for the Nikon AFM microflex shutter to stop working on this model of microscope camera?

The controller works fine and has fresh batteries.

Is this something that can be easily repaired?

If not, who can repair it.

Thank you for any help.

Norman

---------------------------------------------------------------------------

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From: walck-at-southbaytech.com
Date: Mon, 11 Jul 2005 01:18:19 -0500
Subject: [Microscopy] re: Imaging thin films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,

If you can crush, fracture, and polish samples, you are well on your way to
preparing samples by other means. South Bay Technology, as well as our
competitors, sell complete lines of sample preparation equipment for doing
what you want to do. A lot of the expertise of sample prep has been built
into the instruments or is contained in the operating manuals. At the South
Bay Technology web site, www.southbaytech.com, you can find a list of
application notes and technical papers associated with each instrument. At
our competitors' sites, you can find similar information. South Bay
Technology isn't the only company that has an experienced microscopist on
staff (just the best, LOL -Sorry Wolfgang and Rocco. I just couldn't
resist.). There are also short courses that involve sample preparation.
Ron Anderson and I are teaching a one day short course on TEM sample
preparation at the M&M 2005 meeting that you might be interested in
attending. We, as well as our competitors, periodically announce short
courses on general EM preparation techniques and on specific techniques.

For your immediate needs, I would recommend the MicroCleave(TM) technique,
(aka Small Angle Cleavage Technique). This is our model 520 kit and there
is a "how to" instruction guide that I wrote that you can get at our site to
learn how to do this. It is applicable to silicon, III-V and II-V
compounds, SiC, sapphire, GaN, glass, and other brittle materials. It
produces extremely good samples and is fairly easy to learn how to do and to
teach students and doesn't require any more skills that you have already
stated that you have.

For using other sample prep techniques such as dimpling, ion milling, and
Tripod Polishing, you can also find specific information about these
techniques at our website or you can refer to the four MRS proceedings on
TEM Preparation for the Physical Sciences. The common editor in these four
proceedings is Ron Anderson. I am not in my office at the moment, or I
would give you the volume numbers, but I do remember three: 115, 254, and
480.

Another good way of finding out about these techniques is to find a
university with an electron microscopy center that has an experienced lab
director and ask for their advice. A few universities that immediately come
to mind since I visited or used their facilities are Carnegie Mellon Univ,
North Carolina State Univ, Ohio State Univ, Univ of Florida, Univ of
California at Irvine, and Penn State Univ. I hope that I haven't slighted
anyone. The people at these centers have dealt with a multitude of
materials and sample preparation techniques and have a wealth of
information. If they can't help you, they will know who can.

I hope this helps.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: +1-949-492-2600
FAX: +1-949-492-1499
walck-at-southbaytech.com

Disclaimer: South Bay Technology manufactures and sells instrumentation for
the preparation of EM samples.








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From: walck-at-southbaytech.com
Date: Mon, 11 Jul 2005 02:15:11 -0500
Subject: [Microscopy] re: carbon evaporation problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am not familiar with this unit. However, your description sounds like there is a short in the electrodes. Have you checked to see whether evaporated material has put a conductive path between your electrodes? Try putting a current through without the carbon rod to see if you still get a high or even a low current draw. If you do, clean the system.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: +1-949-492-2600
FAX: +1-949-492-1499
walck-at-southbaytech.com

-------- Original Message --------
} From: cgarber-at-2spi.com
} Sent: Saturday, July 09, 2005 4:14 AM
} To: Walck-at-SouthBayTech.com
} Subject: [Microscopy] carbon evaporation problem
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Oldrich Benada wrote:
} =================================================================
} We have troubles with evaporation control unit BSV 202 of our BALZERS BAF
} 301 FE device. Does anybody have some experiences with it?
}
} The current needed for carbon evaporation suddenly jumped so high that the
} 8 A fuse blown out happend. After replacing it, the current needed for
} carbon evaporation stays too high and exceeds the permitted current of
} safeguarding 8A fuse. There are no differences if we use evaporator 1
} (transformer 1) or evaporator 2 (transformer 2).
} For carbon evaporation we use sharpenned carbon rods.
}
} Thanking in advance for any suggestion.





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From: ekman-at-itg.uiuc.edu
Date: Mon, 11 Jul 2005 10:17:15 -0500
Subject: [Microscopy] LM- Light Microscopy Position - Beckman Institute at the University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Applicants are being sought for the position of Specialist Light
Microscopist {jobs/SeniorMicro.htm} in the Imaging Technology Group
(ITG) at the Beckman Institute at the University of Illinois at
Urbana-Champaign.

Information regarding this position can be found here:
http://www.itg.uiuc.edu/jobs/SeniorMicro.htm

More information on what we do at the Imaging Technology Group can be
found here:
http://www.itg.uiuc.edu/

Sincerely,

Jonathan M. Ekman
Beckman Institute for Advanced Science and Technology, Imaging
Technology Group
University of Illinois at Urbana-Champaign
405 N. Mathews Avenue
Urbana, IL 61801 USA
Tel: 217-244-6292
Fax: 217-244-6219


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From: snydert-at-mcmaster.ca
Date: Mon, 11 Jul 2005 12:56:16 -0500
Subject: [Microscopy] viaWWW: JEOL-10S SAM Documentation

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(snydert-at-mcmaster.ca) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
Monday, July 11, 2005 at 12:42:58
---------------------------------------------------------------------------

Email: snydert-at-mcmaster.ca
Name: Tom Snyder

Organization: McMaster University

Title-Subject: [Filtered] MListserver:JEOL-10S SAM Documentation

Question: Hello,

We are trying to get this Auger microscope up and running but are missing documentation about electron-optics and how they should be set. Specifically we are having trouble locating the beam.

If anyone has access to the documentation or knows what the settings should be I would appreciate any feedback. Also if you know of a good test to verify the opticas are working, that would be great info as well.

Thanks
Tom S

---------------------------------------------------------------------------

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From: svanhorn-at-notes.cc.sunysb.edu
Date: Mon, 11 Jul 2005 19:07:50 -0500
Subject: [Microscopy] AskAMicroscopist: DAP Weldwood contact cement

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (svanhorn-at-notes.cc.sunysb.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, July 11, 2005 at 15:01:44
---------------------------------------------------------------------------

Email: svanhorn-at-notes.cc.sunysb.edu
Name: Sue Van Horn

Organization: SUNY-at-StonyBrook

Education: Graduate College

Location: Stony Brook,NY,USA

Question: has anyone ever used DAP Weldwood contact cement mixed with xylene to help in picking up serial sections???..if so how did you use it and at what dilution???
thanks
sue

---------------------------------------------------------------------------

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From: xburany-at-yahoo.com
Date: Tue, 12 Jul 2005 07:44:15 -0500
Subject: [Microscopy] Used LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning.

We want purchase a used optical microscope with
digital camera. Please contact with me directly if you
can help. Thanks,

Meng Burany
mburany-at-uwindsor.ca
519 253-3000 ext.2605



____________________________________________________
Sell on Yahoo! Auctions – no fees. Bid on great items.
http://auctions.yahoo.com/

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From: lcgould-at-med.cornell.edu
Date: Tue, 12 Jul 2005 08:38:21 -0500
Subject: [Microscopy] Re: AskAMicroscopist: DAP Weldwood contact cement

Contents Retrieved from Microscopy Listserver Archives
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Sue,
I don't remember if it was contact cement or some other glue, but I do remember reading (years ago) about painting the top and bottom sides of the block with a dilute "stick-um" so that as the sections were cut, they adhered to one another via the layer of sticky stuff on the edges. I never tried it, and I don't know how, if the sections adhere so well, one is supposed to separate the ribbon into pieces that will fit on a grid.
I know this isn't much help, but at least you know you're not the only one who has heard of such an approach.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: zaluzec-at-microscopy.com
Date: Tue, 12 Jul 2005 09:08:07 -0500
Subject: [Microscopy] Administrivia: Nestor is testing a minor change : 9:07 AM

Contents Retrieved from Microscopy Listserver Archives
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Administrivia: Nestor is testing a minor change : 9:07 AM

ML Version 7

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From: zaluzec-at-microscopy.com
Date: Tue, 12 Jul 2005 09:10:38 -0500
Subject: [Microscopy] Test @ 9:10

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Is the reply to fixed?

9:10

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From: milleri-at-ohio.edu
Date: Tue, 12 Jul 2005 09:18:03 -0500
Subject: [Microscopy] Weldwood cement & serial sections

Contents Retrieved from Microscopy Listserver Archives
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Sue,
The use of Weldwood cement "diluted with one or two parts of commercially
available contact cement thinner" (20% n-butyl acetate, 80% mineral spirits)
to coat the leading and trailing block faces plus many other technique tips
for serial sectioning was published by WH Fahrenbach J. Electron Microscopy
Technique 1:387-398; 1984. See also EC Henry Stain Technol. 52: 59-60; 1977
who I think was first to use contact cement for this purpose


Iain Miller
Department of Biological Sciences
Ohio University
Athens, OH 45701

740-593-2120

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From: chrisjohnrhodes-at-hotmail.com
Date: Tue, 12 Jul 2005 11:07:35 -0500
Subject: [Microscopy] viaWWW: mounting medium

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (chrisjohnrhodes-at-hotmail.com) from on Tuesday, July 12, 2005 at 10:56:47
---------------------------------------------------------------------------

Email: chrisjohnrhodes-at-hotmail.com
Name: Chris Rhodes

Organization: Syracuse University

Title-Subject: [Filtered] MListserver:

Question: I'm looking for a semi-permanent mounting medium with the best possible refractive index match to immersion oil and a cover slip (ie as close as possible to 1.515).

The best I have found so far is 1.539

It is for mounting small (less than 2mm) insect structures.

It will be used for CLSM and thus would hopefully have minimal fluorescence.

---------------------------------------------------------------------------

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From: dsherman-at-purdue.edu
Date: Tue, 12 Jul 2005 12:00:35 -0500
Subject: [Microscopy] Film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Folks,

Due to numerous camera jams over the years we are a bit short on film
cassettes for the Philips CM-10 and CM-100. I would appreciate your
contacting me if any of you have extra cassettes that you are willing to
give/sell to me.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



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6, 20 -- Subject: Film cassettes
6, 20 -- From: Debby Sherman {dsherman-at-purdue.edu}
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From: mgoheen-at-iupui.edu
Date: Tue, 12 Jul 2005 13:37:06 -0500
Subject: [Microscopy] Film cassettes

Contents Retrieved from Microscopy Listserver Archives
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Hi Debby,

I got quite a few from our Philips 300 before we got rid of it. How many
do you need? I use them to replace bad ones in our CM10 and they work
fine.

Mike

Michael P. Goheen
Electron Microscopy Lab
Dept. of Pathology & Lab Medicine
Indiana University School of Medicine
Tel. 317-274-7604
Fax 317-274-5346
mgoheen-at-iupui.edu


-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Tuesday, July 12, 2005 12:04 PM
To: Goheen, Michael P.

Folks,

Due to numerous camera jams over the years we are a bit short on film
cassettes for the Philips CM-10 and CM-100. I would appreciate your
contacting me if any of you have extra cassettes that you are willing to
give/sell to me.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



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6, 20 -- Subject: Film cassettes
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17, 26 -- From mgoheen-at-iupui.edu Tue Jul 12 13:37:06 2005
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From: TindallR-at-missouri.edu
Date: Tue, 12 Jul 2005 17:12:03 -0500
Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

I have another one of my "back to the basics" questions that probably
make people wonder how the devil I ever got into this business in the
first place, but here goes anyway.

We have a client who prepares his own blocks and brings them to us for
sectioning, staining, viewing, etc. One day he came into the lab with a
bunch of sections on copper mesh grids which he had done
immunocytochemistry with standard gold-conjugated secondaries. After
viewing them, I offered him the standard advice that next time we would
mount some sections on nickel or gold grids if he let us know in advance
that he would be doing immunocytochemistry. He said he always used
copper and asked why he should switch. My answer was something like
"Umm-uhhh.....because it's always done that way" with an additional
mumble about copper interfering with the labeling reaction somehow.

Now I have another client, also infinitely more savvy in chemistry that
I am, asking the same question.

So I checked through our little in-house research library (again) and
did some targeted Googling (again) and found that some folks say that
copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
that anyway); 2) may react with PBS buffers to produce fine
precipitates (but, but....doesn't that mean we should NEVER use Cu with
PBS?); 3) reacts with gold colloid (no other explanation given), 4)
can alter the charge distribution of the section and cause non-specific
background label; and 5) may be oxidized during labelling or interfere
with oxidation of chemical groups in the tissue to be labelled. Mostly
people just say to use Ni or Au without stating any reasons.

Also, a glance through the literature shows that it's not uncommon to
find perfectly successful immunolabelling done on copper grids.

Now, if I were determined to invade a country called Copper any or all
of the above reasons could be used with little further explanation, but
I'd like to know the Truth and pass it along to my admiring customers.

Any takers?

Thanks in advance.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







==============================Original Headers==============================
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15, 23 -- Date: Tue, 12 Jul 2005 17:11:47 -0500
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From: Rosemary.White-at-csiro.au
Date: Tue, 12 Jul 2005 17:53:35 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Randy,

I only ran into the "use only Ni or Au grids" rule here, in my current job,
and my predecessor certainly produced some superb images showing gold
labelling of TEM sections. Blissfully unaware of this rule, I had been
using copper grids for all EM immunolabelling. To get good labelling of one
particular structure, which is about 40 nm diameter, I used uncoated
thin-bar Cu grids so I could get labelling on both sides of the section -
seemed to work just fine. I'll be interested to see the responses to your
question.

cheers,
Rosemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


} From: TindallR-at-missouri.edu
} Reply-To: microscopy-at-microscopy.com
} Date: Tue, 12 Jul 2005 17:15:38 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with a
} bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we would
} mount some sections on nickel or gold grids if he let us know in advance
} that he would be doing immunocytochemistry. He said he always used
} copper and asked why he should switch. My answer was something like
} "Umm-uhhh.....because it's always done that way" with an additional
} mumble about copper interfering with the labeling reaction somehow.
}
} Now I have another client, also infinitely more savvy in chemistry that
} I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause non-specific
} background label; and 5) may be oxidized during labelling or interfere
} with oxidation of chemical groups in the tissue to be labelled. Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, but
} I'd like to know the Truth and pass it along to my admiring customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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==============================Original Headers==============================
6, 23 -- From Rosemary.White-at-csiro.au Tue Jul 12 17:53:34 2005
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From: hanke-at-mee-inc.com
Date: Tue, 12 Jul 2005 18:02:49 -0500
Subject: [Microscopy] Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers:

As usual, I am hoping to enlist the vast knowledge and experience of the
this group to solve a perplexing problem. Here is the puzzle.

We have noticed artifact elemental peaks in EDS spectra obtained with
our Oxford Inca system on a Hitachi S-4700 FESEM. For example, an
analysis in the center of a strip of 6 mm wide clean copper tape on an
aluminum stub generates a spectrum with large peaks for copper (this is
good) and a small aluminum peak (not good). Repeat the experiment with a
carbon stub, and the small aluminum peak is replaced by a carbon peak.

Oddly, the phenomenon is observed only with the microscope operating in
High Mag mode. The same analysis (same mag, count rate, etc.) on the
copper tape in Low Mag mode detects only copper. This result is similar
to what we might expect from beam scatter in a variable pressure SEM.

Anyone one out there experienced this problem? Anyone with a similar
microscope willing to try the copper tape experiment to let us know if
this is unique to our instrument or fundamental to the S-4700. Hitachi
and Oxford are perplexed so far.

Thanks for your kind assistance.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


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From: Sally.Stowe-at-anu.edu.au
Date: Tue, 12 Jul 2005 18:07:04 -0500
Subject: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,
I had problems with Cu grids when incubating sections for long periods -
overnight for instance. The copper would react with whatever was available
and form green-blue salts. Not unexpected. We switched to nickel grids for
a while but they charge, gold but they are delicate...by this time we had
shortened the incubation times used, and tried copper again... And had no
problem using coated grids and incubation times of an hour or so. Grids are
floated on drops of media so that only the coated side is wetted - I don't
know if that is important.

Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C



} -----Original Message-----
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} Sent: Wednesday, 13 July 2005 8:12 AM
} To: sally.stowe-at-anu.edu.au
} Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids
}
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From: gary-at-gaugler.com
Date: Tue, 12 Jul 2005 18:30:50 -0500
Subject: [Microscopy] Re: Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What is the analytical WD for EDS in this SEM?
What WD are you using?

gary g.


At 04:04 PM 7/12/2005, you wrote:



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From: dsherman-at-purdue.edu
Date: Tue, 12 Jul 2005 19:08:23 -0500
Subject: [Microscopy] Film cassettes

Contents Retrieved from Microscopy Listserver Archives
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Mike,

I didn't think the ones from the EM series would work in the CM series. Our
EM-400 took the larger plate cassettes with the film inserts. I thought
those inserts were different from the film holders that came with the CM
series but I may be wrong. If they do work than I may still have a few
around as well. I gave away most of my cameras and cassettes from the
EM-400 when we decommissioned it but still may have a few around. I'll check
and let you know if I would like to try the ones you have.

Are you going to M&M2005?

Debby


On 7/12/05 1:40 PM, "mgoheen-at-iupui.edu" {mgoheen-at-iupui.edu} wrote:

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} Hi Debby,
}
} I got quite a few from our Philips 300 before we got rid of it. How many
} do you need? I use them to replace bad ones in our CM10 and they work
} fine.
}
} Mike
}
} Michael P. Goheen
} Electron Microscopy Lab
} Dept. of Pathology & Lab Medicine
} Indiana University School of Medicine
} Tel. 317-274-7604
} Fax 317-274-5346
} mgoheen-at-iupui.edu
}
}
} -----Original Message-----
} X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
} Sent: Tuesday, July 12, 2005 12:04 PM
} To: Goheen, Michael P.
} Subject: [Microscopy] Film cassettes
}
}
}
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} Folks,
}
} Due to numerous camera jams over the years we are a bit short on film
} cassettes for the Philips CM-10 and CM-100. I would appreciate your
} contacting me if any of you have extra cassettes that you are willing to
} give/sell to me.
}
} Thanks,
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
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From: colijn.1-at-osu.edu
Date: Tue, 12 Jul 2005 19:12:25 -0500
Subject: [Microscopy] Re: Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Larry,

I'm not overly familiar with the Hitachi SEMs, but is the "hi-mag" mode on
your SEM an immersion lens mode? This is most likely the case if you are
using an "in-lens" or "in-column" detector. If so, it is possible that the
BSE are being bent around by the immersion field and striking back on the
sample. Depending on the immersion field, this could be close or far from
the beam. These will probably still be within the viewing angle of your
EDS collimator. In their immersion lens SEMs, FEI provides an EDS mode
with a weakened immersion lens field so that the BSE are bent somewhat less
and strike outside the field of view of the EDS collimator.

Cheers,
Henk


At 07:04 PM 7/12/2005, hanke-at-mee-inc.com wrote:



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From: colijn.1-at-osu.edu
Date: Tue, 12 Jul 2005 19:24:12 -0500
Subject: [Microscopy] Re: Film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debbie,

The CM series used both the 36 and 56 sheet film boxes. We have both an
early CM12 which used the 2-part film carriers (36 exposure box; same as
our old EM400) and a CM200 with the thinner single piece film carriers (56
exposure box). I don't know if the EM300 used the same film carriers as
the EM400 series since the camera boxes in the EM300 we had (years ago)
were different. The EM400 and later Philips/FEI scopes use the single
"behind the column" film box assembly

I believe the issue is more the film box than the microscope. ...and more
specifically, the film transport tray since the 2 carriers are different
thicknesses. The boxes have the same outside dimensions and are (I
believe) interchangeable. In other words, I could take my old 36 exposure
film box, drop it right into my CM200 and only need to change the film
stock number. CAUTION -- I haven't actually tested this since I haven't
felt motivated to reduce the number of exposures in my microscope!

Cheers,
Henk


At 08:10 PM 7/12/2005, dsherman-at-purdue.edu wrote:

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From: dsherman-at-purdue.edu
Date: Tue, 12 Jul 2005 19:46:33 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

We used Cu grids for many years. Normally we use formvar + carbon coated
grids for ICC since we have a lot less loss of sections. We also tend to do
long incubations ...usually overnight. This is time efficient and also lets
us use highly diluted antibody so also minimize background due to
cross-reactions from contaminants in polyclonal antibodies.

Occasionally we would have a reaction due to copper oxidizing with resultant
green solution. I attributed this to salts or traces of Tween 20 in the
buffer and breaks in the coating exposing the Cu. As far as I can see, this
is the only reason for not using Cu grids if you use coated grids. We have
switched for the most part to using coated Ni grids to avoid this problem.

On the other hand, occasionally we need to do a double labeling using
uncoated grids and both sides of the sections. I would hesitate to use Cu
for this and prefer Ni. Gold would work but is both more expensive and more
delicate than Ni grids.

Debby


Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 7/12/05 5:15 PM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} wrote:

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} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with a
} bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we would
} mount some sections on nickel or gold grids if he let us know in advance
} that he would be doing immunocytochemistry. He said he always used
} copper and asked why he should switch. My answer was something like
} "Umm-uhhh.....because it's always done that way" with an additional
} mumble about copper interfering with the labeling reaction somehow.
}
} Now I have another client, also infinitely more savvy in chemistry that
} I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause non-specific
} background label; and 5) may be oxidized during labelling or interfere
} with oxidation of chemical groups in the tissue to be labelled. Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, but
} I'd like to know the Truth and pass it along to my admiring customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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12, 21 -- From dsherman-at-purdue.edu Tue Jul 12 19:46:33 2005
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12, 21 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids
12, 21 -- From: Debby Sherman {dsherman-at-purdue.edu}
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From: paul_hazelton-at-umanitoba.ca
Date: Tue, 12 Jul 2005 22:55:26 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The film holders for the Philips EM400 and the CM series are the same. The
same holders can be used in the Philips EM201,EM300, EM301 . With the
inserts
you load cut film without you load glass plates.
----- Original Message -----
X-from: {dsherman-at-purdue.edu}
To: {amtecss-at-earthlink.net}
Sent: Tuesday, July 12, 2005 8:08 PM

randy

you don't say what your client's stuff looked like. that would help.

had a former director, before i grew up and went back to school - or was
that truly growing up? anyway, out of nickel grids, not to mention
formvar-nickel. she wanted IEM done. then! forthwith! no delay! do
not put it of! threat of discipline if not in her hands that
afternoon!! etc. insisted i use copper. this was also on formvar
coated grids. not pretty, quite nasty, large semicrystals of junk all
over. really did not look good after the second and third try.

there were several possible problems. first, we spun things onto the
grid, 30 minutes in an air driven ultracentrifuge. so the copper back
surface was guaranteed to get wet and react with the buffer. or perhaps
it was the buffer she had things in, i never could get a straight answer
from her on that one.

recently i did a long ultracentrifuge run to load some 10S particles
onto a formvar-copper grid. the grids also reacted with the phosphate
buffer over the 4 hour spin time. again, not a pretty picture - i had
to go back to my collaborator and get fresh material so we could put it
onto formvar-nickel grids. wonderful gentleman. much more mature.
real pleasure to collaborate with.

having said that, i read what rosemary white, sally stowe and debbie
sherman have said. i make no pretense about my opinion on dogma
(dogma-schmogma, it ain't gospel until i try it and prove the fact to me
personally). give it a go and let the rest of us try out to see what
happens. perhaps i will even try it in my overworked 10 finger lab.
but after the two molecular experiments i need to do in order get that
paper of mine accepted on resubmission, and the 7 other collaborators'
projects, the looking at micrographs from 2 outside sources with
questions, etc. all right, i exagerate, it's not quite that bad but i
really do have to get back to one person about his results before i go
on holiday tomorrow.

give it a shot and let us know

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926


==============================Original Headers==============================
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From: eprincipe01-at-hotmail.com
Date: Tue, 12 Jul 2005 23:22:57 -0500
Subject: [Microscopy] Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

With magnetic field active, test stray signal as a function of WD. Same experiment with a charge sensitive sample should also be interesting, not for x-ray, but SE. response.




-----Original Message-----
X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com}
Sent: Tuesday, July 12, 2005 11:03 PM
To: "eprincipe01-at-hotmail.com" {eprincipe01-at-hotmail.com}

Dear Listers:

As usual, I am hoping to enlist the vast knowledge and experience of the
this group to solve a perplexing problem. Here is the puzzle.

We have noticed artifact elemental peaks in EDS spectra obtained with
our Oxford Inca system on a Hitachi S-4700 FESEM. For example, an
analysis in the center of a strip of 6 mm wide clean copper tape on an
aluminum stub generates a spectrum with large peaks for copper (this is
good) and a small aluminum peak (not good). Repeat the experiment with a
carbon stub, and the small aluminum peak is replaced by a carbon peak.

Oddly, the phenomenon is observed only with the microscope operating in
High Mag mode. The same analysis (same mag, count rate, etc.) on the
copper tape in Low Mag mode detects only copper. This result is similar
to what we might expect from beam scatter in a variable pressure SEM.

Anyone one out there experienced this problem? Anyone with a similar
microscope willing to try the copper tape experiment to let us know if
this is unique to our instrument or fundamental to the S-4700. Hitachi
and Oxford are perplexed so far.

Thanks for your kind assistance.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


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From: YANGA-at-AGR.GC.CA
Date: Wed, 13 Jul 2005 08:24:44 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I had a bad experience with formvar-copper grids in immunogold experiment many years ago when incubating primary antibody overnight and the rest done on the following morning. I am using Nickel grids now -- no more problems.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au]
Sent: Tuesday, July 12, 2005 6:55 PM
To: Yang, Ann-Fook

Dear Randy,

I only ran into the "use only Ni or Au grids" rule here, in my current job,
and my predecessor certainly produced some superb images showing gold
labelling of TEM sections. Blissfully unaware of this rule, I had been
using copper grids for all EM immunolabelling. To get good labelling of one
particular structure, which is about 40 nm diameter, I used uncoated
thin-bar Cu grids so I could get labelling on both sides of the section -
seemed to work just fine. I'll be interested to see the responses to your
question.

cheers,
Rosemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


} From: TindallR-at-missouri.edu
} Reply-To: microscopy-at-microscopy.com
} Date: Tue, 12 Jul 2005 17:15:38 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with a
} bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we would
} mount some sections on nickel or gold grids if he let us know in advance
} that he would be doing immunocytochemistry. He said he always used
} copper and asked why he should switch. My answer was something like
} "Umm-uhhh.....because it's always done that way" with an additional
} mumble about copper interfering with the labeling reaction somehow.
}
} Now I have another client, also infinitely more savvy in chemistry that
} I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause non-specific
} background label; and 5) may be oxidized during labelling or interfere
} with oxidation of chemical groups in the tissue to be labelled. Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, but
} I'd like to know the Truth and pass it along to my admiring customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
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From: gwe-at-ufl.edu
Date: Wed, 13 Jul 2005 08:39:16 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have had problems with Cu grids, even Formvar coated ones. I have
precoated copper grids with Parlodion by dipping, blotting and drying
before use, with or with out a formvar coating. THat method seems to
protect the copper from reacting with PBS or whatever. This is not
original to me, but I cannot recall where I read this (among many other
things I can no longer recall). I think it might have been one of those
smart Swiss guys.

Greg

TindallR-at-missouri.edu wrote:

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From: marc.pypaert-at-yale.edu
Date: Wed, 13 Jul 2005 10:37:12 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What's the big deal about preferring Cu to Ni anyway?
Right, there is the charging problem with Nickel, but if
you use anti-magnetic tweezers they are just as easy
to handle than copper. And the price is really not that
much more! We sometimes have to leave grids
overnight or longer on solutions, so to be safe we use
nickel for this. Since we don't want to make and keep
separate stocks of copper and nickel, we switched
entirely to nickel grids, and have had no problems with
any of our applications. Am I missing something here?!!

Marc

On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote:

}
}
}
} -----------------------------------------------------------------------
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}
} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with
} a
} bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we would
} mount some sections on nickel or gold grids if he let us know in
} advance
} that he would be doing immunocytochemistry. He said he always used
} copper and asked why he should switch. My answer was something like
} "Umm-uhhh.....because it's always done that way" with an additional
} mumble about copper interfering with the labeling reaction somehow.
}
} Now I have another client, also infinitely more savvy in chemistry that
} I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause non-specific
} background label; and 5) may be oxidized during labelling or
} interfere
} with oxidation of chemical groups in the tissue to be labelled.
} Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, but
} I'd like to know the Truth and pass it along to my admiring customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original
} Headers==============================
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}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
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6, 18 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids
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From: TindallR-at-missouri.edu
Date: Wed, 13 Jul 2005 10:56:21 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marc,

It doesn't make a lot of difference to me either way, except to be able
to actually have an intelligent explanation when our users ask questions
about our methods. That said, there are times when we do get severe
image distortion in our TEM when using Ni grids with a healthy charge on
them. But my main motivation was curiosity, nada mas.

Randy

-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Wednesday, July 13, 2005 10:39 AM
To: Tindall, Randy D.

What's the big deal about preferring Cu to Ni anyway?
Right, there is the charging problem with Nickel, but if you use
anti-magnetic tweezers they are just as easy to handle than copper. And
the price is really not that much more! We sometimes have to leave grids
overnight or longer on solutions, so to be safe we use nickel for this.
Since we don't want to make and keep separate stocks of copper and
nickel, we switched entirely to nickel grids, and have had no problems
with any of our applications. Am I missing something here?!!

Marc

On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote:

}
}
}
} ----------------------------------------------------------------------
} -
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -
} -----
}
} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for

} sectioning, staining, viewing, etc. One day he came into the lab with

} a bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we
} would mount some sections on nickel or gold grids if he let us know in

} advance that he would be doing immunocytochemistry. He said he always

} used copper and asked why he should switch. My answer was something
} like "Umm-uhhh.....because it's always done that way" with an
} additional mumble about copper interfering with the labeling reaction
} somehow.
}
} Now I have another client, also infinitely more savvy in chemistry
} that I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu
with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause
} non-specific background label; and 5) may be oxidized during
} labelling or interfere
} with oxidation of chemical groups in the tissue to be labelled.
} Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all

} of the above reasons could be used with little further explanation,
} but I'd like to know the Truth and pass it along to my admiring
customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original
} Headers==============================
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--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original
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Pypaert {marc.pypaert-at-yale.edu} 6, 18 -- Subject: Re: [Microscopy] TEM:
Immunocytochemistry and Cu grids 6, 18 -- In-reply-to:
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From: ehaller-at-hsc.usf.edu
Date: Wed, 13 Jul 2005 11:22:28 -0500
Subject: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

The main problem encountered with copper grids used for
immunocytochemistry or immunogold labeling is the reaction of the copper
with salts in the buffers used during labeling. This is a time-related
reaction, and can usually be avoided by having short labeling runs and
using grids with films on them. Gilder grids, available from major
microscope supply vendors, are gold-coated copper grids, and are not
that much more expensive than regular copper grids. These grids may be
the variety used by researchers who have reported having no problems
with their grids during their immuno runs.
I usually use nickel grids for my work, but have used
formvar-coated copper grids without problems for 1 day immuno runs so
long as the film is intact and as long as I don't get clumsy and end up
sinking my grids in my solutions (if I do sink them, I do a quick
distilled water rinse, blot the back of the grids with filter paper
until almost dry, then re-float them where I left off). As others have
shared, the nickel grids are much sturdier, not too expensive, and are
not a problem to handle as long as you use antimagnetic forceps. With
the nickel grids, remember to correct for astigmatism in the TEM by
using a hole in your sample on the nickel grid. This will accommodate
for any inherent residual magnetic field in the grid itself.

Edward Haller
Lab Manager, Diagnostic Electron Microscopy Lab
University of South Florida
Pathology Department
Tampa, FL 33612
(813) 974-9584


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From: marc.pypaert-at-yale.edu
Date: Wed, 13 Jul 2005 12:01:03 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,

Sorry for any misunderstanding - My response was not
really directed at you, but at the EM community in general
who for some reason seems to be biased towards copper.
I have never understood this. I have been doing EM for
nearly 20 years, exclusively using nickel grids, and would
really like to find out if I was mistaken this entire time!! Or
is it just another case of "dogma", like Paul put in nicely
in his posting?
Anyway, I will read with interest the responses you get
to your posting!
Best

Marc

On Wednesday, July 13, 2005, at 11:57 AM, TindallR-at-missouri.edu wrote:

}
}
}
} -----------------------------------------------------------------------
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}
} Marc,
}
} It doesn't make a lot of difference to me either way, except to be able
} to actually have an intelligent explanation when our users ask
} questions
} about our methods. That said, there are times when we do get severe
} image distortion in our TEM when using Ni grids with a healthy charge
} on
} them. But my main motivation was curiosity, nada mas.
}
} Randy
}
} -----Original Message-----
} X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
} Sent: Wednesday, July 13, 2005 10:39 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids
}
}
}
}
} -----------------------------------------------------------------------
} -
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}
} What's the big deal about preferring Cu to Ni anyway?
} Right, there is the charging problem with Nickel, but if you use
} anti-magnetic tweezers they are just as easy to handle than copper. And
} the price is really not that much more! We sometimes have to leave
} grids
} overnight or longer on solutions, so to be safe we use nickel for this.
} Since we don't want to make and keep separate stocks of copper and
} nickel, we switched entirely to nickel grids, and have had no problems
} with any of our applications. Am I missing something here?!!
}
} Marc
}
} On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------
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} }
} } Dear Listers,
} }
} } I have another one of my "back to the basics" questions that probably
} } make people wonder how the devil I ever got into this business in the
} } first place, but here goes anyway.
} }
} } We have a client who prepares his own blocks and brings them to us for
}
} } sectioning, staining, viewing, etc. One day he came into the lab with
}
} } a bunch of sections on copper mesh grids which he had done
} } immunocytochemistry with standard gold-conjugated secondaries. After
} } viewing them, I offered him the standard advice that next time we
} } would mount some sections on nickel or gold grids if he let us know in
}
} } advance that he would be doing immunocytochemistry. He said he always
}
} } used copper and asked why he should switch. My answer was something
} } like "Umm-uhhh.....because it's always done that way" with an
} } additional mumble about copper interfering with the labeling reaction
} } somehow.
} }
} } Now I have another client, also infinitely more savvy in chemistry
} } that I am, asking the same question.
} }
} } So I checked through our little in-house research library (again) and
} } did some targeted Googling (again) and found that some folks say that
} } copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} } that anyway); 2) may react with PBS buffers to produce fine
} } precipitates (but, but....doesn't that mean we should NEVER use Cu
} with
} } PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} } can alter the charge distribution of the section and cause
} } non-specific background label; and 5) may be oxidized during
} } labelling or interfere
} } with oxidation of chemical groups in the tissue to be labelled.
} } Mostly
} } people just say to use Ni or Au without stating any reasons.
} }
} } Also, a glance through the literature shows that it's not uncommon to
} } find perfectly successful immunolabelling done on copper grids.
} }
} } Now, if I were determined to invade a country called Copper any or all
}
} } of the above reasons could be used with little further explanation,
} } but I'd like to know the Truth and pass it along to my admiring
} customers.
} }
} } Any takers?
} }
} } Thanks in advance.
} }
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} }
} }
} }
} }
} }
} }
} }
} } ==============================Original
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} }
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original
} Headers==============================
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--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
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From: tonygr-at-MIT.EDU
Date: Wed, 13 Jul 2005 12:32:00 -0500
Subject: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Over the years I have frequently seen cases where the samples themselves
have reacted with the copper grid. Two examples:

1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
precipitates were ugly and analyzed as copper sulphide. Our surmise was
that the iron and copper had undergone ion exchange while the grid (and
carbon film) was wet with the culture medium. When we used nickel grids,
the precipitates were well formed and composed of iron and sulphur.

2) Looking at Si precipitates in Al-Si electronic bond wire. The
specimens were prepared by embedding and microtoming, floating in DI
water. The Si precipitates were surrounded by an ugly mess containing
masses of copper. Again, presumed to me electrochemical reaction between
the Al and the Cu grid, through the DI water medium, somehow preferentially
at the Si precipitates. Again, use of Ni grids produced good pictures
allowing us to characterize the precipitation.

Having said all that, we continue to default to use copper grids!

Tony.


***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


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From: MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 13 Jul 2005 12:51:31 -0500
Subject: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for your weigh-in on this subject. I was sorry to learn of your hearing
problems; has this been coming on gradually or did something happen (i.e. a
severe cold, etc.)to bring the hearing loss on?

I was surprised to learn that Boston is not yet out of the running for an MSA
meeting! If I can help in any way, I'll throw my hat in the ring.

Hope your summer (now that it is the middle of July!) is going well.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU]
Sent: Wednesday, July 13, 2005 1:33 PM
To: Sherwood, Margaret

Over the years I have frequently seen cases where the samples themselves
have reacted with the copper grid. Two examples:

1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
precipitates were ugly and analyzed as copper sulphide. Our surmise was
that the iron and copper had undergone ion exchange while the grid (and
carbon film) was wet with the culture medium. When we used nickel grids,
the precipitates were well formed and composed of iron and sulphur.

2) Looking at Si precipitates in Al-Si electronic bond wire. The
specimens were prepared by embedding and microtoming, floating in DI
water. The Si precipitates were surrounded by an ugly mess containing
masses of copper. Again, presumed to me electrochemical reaction between
the Al and the Cu grid, through the DI water medium, somehow preferentially
at the Si precipitates. Again, use of Ni grids produced good pictures
allowing us to characterize the precipitation.

Having said all that, we continue to default to use copper grids!

Tony.


***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


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From: tpepper-at-iastate.edu
Date: Wed, 13 Jul 2005 13:54:01 -0500
Subject: [Microscopy] viaWWW: Help with Fixative near Plainview Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(tpepper-at-iastate.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
on Wednesday, July 13, 2005 at 09:21:59
---------------------------------------------------------------------------

Email: tpepper-at-iastate.edu
Name: Tracey Pepper

Organization: Iowa State University

Title-Subject: [Filtered] MListserver:

Question: HELP! We are in desparate need of some assisitance! We have a scientist in Texas who is in the field collecting crucial samples for TEM examination. They must be collected in the next couple of days. Our efforts to send (via DHL) good fixative (standard em fix for plants a double aldehyde in a 0.1 M cacodylate) have failed! The person is located in Plainview, Texas. Is there anyone on this listserver who could make us some fixtive (150 mls)for this person to come pick up? Preferably within an hour radius? If you can, Please call 515-294-3872 as soon as possible. We would be in your debt!!
With Great appreciation,
Tracey Pepper
Bessey Microscopy Facility
Iowa State University
Ames, IA 50011-1020

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: gcc-at-couger.com
Date: Wed, 13 Jul 2005 15:01:27 -0500
Subject: [Microscopy] Re: viaWWW: Help with Fixative near Plainview Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Your best bet would be to call some one at Texas Tech in Lubbock
http://www.macmed.ttuhsc.edu/ They may be more than an hour from
Plainview but that close to the edge of the world. Your only
other chance is Amarillo with the TAM Vet Med Diagnostic lab
http://www.medcenter.org/facilities/am-vetlab.html.

Good luck
Gordon

Gordon Couger
I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

tpepper-at-iastate.edu wrote:
}
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It was submitted by
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} on Wednesday, July 13, 2005 at 09:21:59
}
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}
} Email: tpepper-at-iastate.edu
} Name: Tracey Pepper
}
} Organization: Iowa State University
}
} Title-Subject: [Filtered] MListserver:
}
} Question: HELP! We are in desparate need of some
assisitance! We have a scientist in Texas who is in the field
collecting crucial samples for TEM examination. They must be
collected in the next couple of days. Our efforts to send (via
DHL) good fixative (standard em fix for plants a double aldehyde
in a 0.1 M cacodylate) have failed! The person is located in
Plainview, Texas. Is there anyone on this listserver who could
make us some fixtive (150 mls)for this person to come pick up?
Preferably within an hour radius? If you can, Please call
515-294-3872 as soon as possible. We would be in your debt!!
} With Great appreciation,
} Tracey Pepper
} Bessey Microscopy Facility
} Iowa State University
} Ames, IA 50011-1020
}
}
---------------------------------------------------------------------------
}
} ==============================Original
Headers==============================
} 6, 12 -- From zaluzec-at-microscopy.com Wed Jul 13 13:54:01 2005
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==============================Original Headers==============================
5, 20 -- From gcc-at-couger.com Wed Jul 13 15:01:27 2005
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From: andrew.mcnaughton-at-stonebow.otago.ac.nz
Date: Wed, 13 Jul 2005 15:43:43 -0500
Subject: [Microscopy] Nikon Diaphot Epi-Fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

Does anyone have a Nikon Diaphot epi-fluorescence lamp house and filter
set/holder for sale? Alternatively, if anyone knows of a second hand
microscope parts vendor?

Thanks for your help.

Regards

Andrew



________________________________________________________________________
______
Andrew McNaughton
Otago Centre for Confocal Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: +64 3 479 7308
Facsimile: +64 3 479 7254

Confocal/EM Centres: http://occm.otago.ac.nz/

Department: http://anatomy.otago.ac.nz/

New Zealand Microscopy: http://microscopynz.otago.ac.nz/

________________________________________________________________________
______


==============================Original Headers==============================
14, 19 -- From andrew.mcnaughton-at-stonebow.otago.ac.nz Wed Jul 13 15:43:43 2005
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14, 19 -- Subject: Nikon Diaphot Epi-Fluorescence
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From: sryazant-at-ucla.edu
Date: Wed, 13 Jul 2005 16:09:43 -0500
Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am using titanium tweezers with nickel grids - they are cheap nowadays
and I like them (tweezers) for light weight and soft action. I am using
carbon coating on top of all my plastic films (copper or nickel grids) and
have no problems with charge/astigmatism. I suspect, charging problem
happens when grids quite old and/or dirty. Sergey

At 09:23 AM 7/13/2005, you wrote:



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==============================Original Headers==============================
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From: jfb-at-uidaho.edu
Date: Wed, 13 Jul 2005 16:27:34 -0500
Subject: [Microscopy] Re: viaWWW: Help with Fixative near Plainview

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John Oren, Vermontoptech 802 425 2040
----- Original Message -----
X-from: {andrew.mcnaughton-at-stonebow.otago.ac.nz}
To: {micro-at-superlink.net}
Sent: Wednesday, July 13, 2005 4:43 PM

Actually, TTU is only about 45 minutes from Plainview, and both the Dept. of
Bio. Sci. and the TTU Medical School have electron microscopy labs.

-----Original Message-----
X-from: gcc-at-couger.com [mailto:gcc-at-couger.com]
Sent: Wednesday, July 13, 2005 1:05 PM
To: jfb-at-uidaho.edu

Your best bet would be to call some one at Texas Tech in Lubbock
http://www.macmed.ttuhsc.edu/ They may be more than an hour from
Plainview but that close to the edge of the world. Your only
other chance is Amarillo with the TAM Vet Med Diagnostic lab
http://www.medcenter.org/facilities/am-vetlab.html.

Good luck
Gordon

Gordon Couger
I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

tpepper-at-iastate.edu wrote:
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} on Wednesday, July 13, 2005 at 09:21:59
}
---------------------------------------------------------------------------
}
} Email: tpepper-at-iastate.edu
} Name: Tracey Pepper
}
} Organization: Iowa State University
}
} Title-Subject: [Filtered] MListserver:
}
} Question: HELP! We are in desparate need of some
assisitance! We have a scientist in Texas who is in the field
collecting crucial samples for TEM examination. They must be
collected in the next couple of days. Our efforts to send (via
DHL) good fixative (standard em fix for plants a double aldehyde
in a 0.1 M cacodylate) have failed! The person is located in
Plainview, Texas. Is there anyone on this listserver who could
make us some fixtive (150 mls)for this person to come pick up?
Preferably within an hour radius? If you can, Please call
515-294-3872 as soon as possible. We would be in your debt!!
} With Great appreciation,
} Tracey Pepper
} Bessey Microscopy Facility
} Iowa State University
} Ames, IA 50011-1020
}
}
---------------------------------------------------------------------------
}
} ==============================Original
Headers==============================
} 6, 12 -- From zaluzec-at-microscopy.com Wed Jul 13 13:54:01 2005
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} 6, 12 -- Subject: viaWWW: Help with Fixative near Plainview Texas
} 6, 12 -- Content-Type: text/plain; charset="us-ascii"
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==============================Original Headers==============================
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14, 27 -- From jfb-at-uidaho.edu Wed Jul 13 16:27:33 2005
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From: r-holdford-at-ti.com
Date: Wed, 13 Jul 2005 17:39:30 -0500
Subject: [Microscopy] Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Henk: I believe you are correct. The "hi-mag" mode does use an in-lens
detector and you will get x-ray peaks you are not wanting due to the way
the magnetic lens acts.

Larry: using "Analysis" mode will correct this problem. The WD for
this scope with an INCA detector should be about 12mm +/- 1mm. Using
the "hi-mag" mode for EDX work is not recommended; you won't get good
resolution at this working distance anyway and the in-lens detector
won't give a very good image at this distance, besides being very
sensitive to charging.

colijn.1-at-osu.edu wrote:

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--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


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6, 23 -- Subject: Re: [Microscopy] Re: Stray X-rays in FE SEM?
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From: xuy-at-nih.gov
Date: Thu, 14 Jul 2005 08:26:00 -0500
Subject: [Microscopy] viaWWW: digital camera for my light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The actual retail price of T221 in the summer of 2004 (when T221 was
available from regular retail sources) was only $4K. Proper video adapters
are $800 to $1,200, largely matter of taste. It is safe to say the whole
deal was approximately. $5K. Totally worth it IMO. The figures around $8K to
$10K discussed in this thread are MSRP, not retail prices.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
X-from: {Mike.Bode-at-soft-imaging.net}
To: {vitalylazar-at-att.net}
Sent: Friday, July 08, 2005 11:01 AM

Hi John,

The advantages besides the obvious superb display are:

1) the ability to display entire image at 100% zoom (pixel-to-pixel
sensor-to-monitor) without zoom or pan;

2) have such image and multiple toolbars and control boxes displayed on the
desktop without interfering with each other, plus an extra application or
two open at the same time, again, not hiding behind other open windows-
makes it much easier on the operator.

Works very well for 4 or 6 megapixels camera. Now, for larger sensor such as
11 or 16 megapixels, one would have to zoom out to 50% in order to see
entire image at once. Still much better than zooming down to 25% on a
regular monitor.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
X-from: {john.mardinly-at-intel.com}
To: {vitalylazar-at-att.net}
Sent: Friday, July 08, 2005 4:16 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (xuy-at-nih.gov) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, July 14, 2005 at 08:09:33
---------------------------------------------------------------------------

Email: xuy-at-nih.gov
Name: Yuhui Xu

Organization: Harvard Medical School

Title-Subject: [Filtered] MListserver:

Question: I am in the process of buying a digital camera for my light microscope ( Leica DM LB2), and would like to get your opinion as to which maker or model is a good choice in terms of resolution, stability, and ease of use, and of course the price.

Thank you.

---------------------------------------------------------------------------

==============================Original Headers==============================
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7, 12 -- Subject: viaWWW: digital camera for my light microscope
7, 12 -- Content-Type: text/plain; charset="us-ascii"
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From: j.wittig-at-vanderbilt.edu
Date: Thu, 14 Jul 2005 08:32:41 -0500
Subject: [Microscopy] viaWWW: Exhibitor Tutorials at MM2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Once again the MSA Education Committee has organized Exhibitor Demonstrations and Tutorials during the Microscopy and Microanalysis 2005 meeting. This will occur on Tuesday, August 2 starting at 5:00 pm in the Exhibit Hall. These mini-seminars and/or tutorial demonstrations are held in the booths of the participating companies after the Hall is closed to non-participants.

Reservations sheets with titles and descriptions will be at the MSA Education table in the MSA Mega Booth. When you sign up you will be issued a ticket, which you will need to renter the Hall after it is closed. You need to sign up no later than noon on Tuesday. The number of attendees is limited, so visit the MSA Education table soon, since the demonstrations get filled up quickly!

Here's a list of participating Exhibitors and titles:

http://mm2005.microscopy.org/MMExhibitorsTutorialsDemos.html


Jim Wittig
MSA Education Committee

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From: nholson-at-ucsd.edu
Date: Thu, 14 Jul 2005 13:52:19 -0500
Subject: [Microscopy] Illuminator for old Wild light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Your problem is simply one of backscatter bouncing off the final lens
irradiating areas off axis, generating the x-rays as appropriate. This is
not just a problem with the SEM you mention, it is a general problem of
which many people are unaware. The worst case I have ever seen was
obtaining x-ray information from 0.75cm (~1/4inch) from the point of initial
beam impact!

Modern detectors and collimators do help but scatter is always a problem. I
believe that is why SEM manufacturer's main holder is often one which places
the specimen surface at a higher level well away from the holder/stage
interface. A specimen "in space" will always offer a cleaner x-ray signal.

It is a personal suggestion to clients that when trying hard with an
analysis they DO NOT use a multi specimen holder!

Those who wish to know more should read texts relating to the production of
SE in relation to those produced by "bounce off" backscatter. We have a
short piece in the "Hints and Tips" section of our web site.

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
X-from: {hanke-at-mee-inc.com}
To: {protrain-at-emcourses.com}
Sent: Wednesday, July 13, 2005 12:03 AM

We have an old Wild MTr19 illuminator for an equally old Wild model 20
compound light microscope. The bulb in the illuminator burnt out and from
the sources I checked that type of bulb is supposedly no longer made. Also,
there are no markings on the bulb to indicate what it is. Is anyone
familiar with this old illuminator and either knows of bulb replacements or
a compatible illuminator for which I can still get bulbs?

Thanks

Norm Olson
______________________________________________________________
Norm Olson
Cryoelectron Microscopy Facilities Manager
4107 Natural Science Building
Department of Chemistry & Biochemistry, MC-0378
University of California San Diego
La Jolla, CA 92093-0378
nholson-at-ucsd.edu
http://cryoem.ucsd.edu
(858)534-5852 ­ Office; (858)534-5846 - Fax
______________________________________________________________







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9, 24 -- Date: Thu, 14 Jul 2005 11:24:03 -0700
9, 24 -- Subject: Illuminator for old Wild light microscope
9, 24 -- From: Norman Olson {nholson-at-ucsd.edu}
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From: cpetty1-at-umbc.edu
Date: Thu, 14 Jul 2005 14:47:51 -0500
Subject: [Microscopy] Re: Illuminator for old Wild light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joe Mears knows everything you what to know about the Swiss made Wild.
Microscope Services 1403 Bradley Avenue, Rockville, Maryland 20851
Office 301.294.7960 Fax 301.294.1934 Cell 240.994.7191

Joe also works on Leica, Zeiss, and Nikon light scopes. He is a very
good repair person.


nholson-at-ucsd.edu wrote:
} ----------------------------------------------------------------------------
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} We have an old Wild MTr19 illuminator for an equally old Wild model 20
} compound light microscope. The bulb in the illuminator burnt out and from
} the sources I checked that type of bulb is supposedly no longer made. Also,
} there are no markings on the bulb to indicate what it is. Is anyone
} familiar with this old illuminator and either knows of bulb replacements or
} a compatible illuminator for which I can still get bulbs?
}
} Thanks
}
} Norm Olson
} ______________________________________________________________
} Norm Olson
} Cryoelectron Microscopy Facilities Manager
} 4107 Natural Science Building
} Department of Chemistry & Biochemistry, MC-0378
} University of California San Diego
} La Jolla, CA 92093-0378
} nholson-at-ucsd.edu
} http://cryoem.ucsd.edu
} (858)534-5852 ­ Office; (858)534-5846 - Fax
} ______________________________________________________________
}
}
}
}
}
}
}
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} 9, 24 -- Date: Thu, 14 Jul 2005 11:24:03 -0700
} 9, 24 -- Subject: Illuminator for old Wild light microscope
} 9, 24 -- From: Norman Olson {nholson-at-ucsd.edu}
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--
Chere Petty, M.S.
Manager, Keith R. Porter Imaging Facility
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
1000 Hilltop Circle
Baltimore, MD 21250
Phone: 410-455-2296
Fax: 410-455-3875

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From: gary.m.brown-at-exxonmobil.com
Date: Thu, 14 Jul 2005 16:37:50 -0500
Subject: [Microscopy] Re: Illuminator for old Wild light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

"The statements and opinions expressed here by Gary M. Brown represent
neither those of ExxonMobil Corporation nor its affiliates."


Norm,

Leica should have a part number for the bulb and the vital stats needed to
purchase it elsewhere.

Regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Technology & Engineering - West
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: 281 834 2387
fax: 281 834 2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



nholson-at-ucsd.e
du
To
gary.m.brown-at-exxonmobil.com
07/14/05 01:54 cc
PM
Subject
[Microscopy] Illuminator for old
Please respond Wild light microscope
to
microscopy-at-mic
roscopy.com










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We have an old Wild MTr19 illuminator for an equally old Wild model 20
compound light microscope. The bulb in the illuminator burnt out and from
the sources I checked that type of bulb is supposedly no longer made.
Also,
there are no markings on the bulb to indicate what it is. Is anyone
familiar with this old illuminator and either knows of bulb replacements or
a compatible illuminator for which I can still get bulbs?

Thanks

Norm Olson
______________________________________________________________
Norm Olson
Cryoelectron Microscopy Facilities Manager
4107 Natural Science Building
Department of Chemistry & Biochemistry, MC-0378
University of California San Diego
La Jolla, CA 92093-0378
nholson-at-ucsd.edu
http://cryoem.ucsd.edu
(858)534-5852 ­ Office; (858)534-5846 - Fax
______________________________________________________________







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From: john.bonevich-at-nist.gov
Date: Fri, 15 Jul 2005 12:11:10 -0500
Subject: [Microscopy] Gatan Duomill service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Does anyone know of a company that can service an old Gatan Duomill? Ours
has developed an electrical problem that keeps the guns from energizing.

Thanks in advance.

-----------------------------------------
John Bonevich, Ph.D.
National Institute of Standards and Technology
Metallurgy Division, Mail-Stop 8555
100 Bureau Drive
Gaithersburg, MD 20899 USA


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From: curulli-at-usc.edu
Date: Fri, 15 Jul 2005 12:43:29 -0500
Subject: [Microscopy] Gatan Duomill service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try

MAS
Art McCanna
Suwanee, Georgia
800-421-8451

-----Original Message-----
X-from: john.bonevich-at-nist.gov [mailto:john.bonevich-at-nist.gov]
Sent: Friday, July 15, 2005 10:15 AM
To: curulli-at-usc.edu

Hello,

Does anyone know of a company that can service an old Gatan Duomill? Ours
has developed an electrical problem that keeps the guns from energizing.

Thanks in advance.

-----------------------------------------
John Bonevich, Ph.D.
National Institute of Standards and Technology
Metallurgy Division, Mail-Stop 8555
100 Bureau Drive
Gaithersburg, MD 20899 USA


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From: rcbaker-at-eden.infohwy.com
Date: Sat, 16 Jul 2005 10:57:37 -0500
Subject: [Microscopy] Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am an amateur and have been experimenting for quite some time with
a technique for sharpening silicon carbide (SiC) crystals as an
alternative to the very expensive diamond knives commonly used with
ultramicrotomes.

The major difficulty is that SiC crystals are very brittle; thus SiC
crystals need to be lapped and polished by unconventional methods to
prevent micro-mechanical stresses from breaking the edge.
Conventional gem faceting technology does not work.

Vitreous carbon knives have also been reported in the literature but
did not live up to expectations apparently.

As many on this list will know, glass knives are preferred for their
initial sharpness. Meanwhile diamond knives are preferred for their
unexcelled durability, flat faces and straight edges, which makes it
possible to make almost unlimited numbers of serial sections up to a
few millimeters wide.

My efforts indicate that it is possible to make SiC knives and that
they can generate silvery sections down to 100 nanometers or so,
which puts them in the ball park for practical EM work. My sections
do transmit electrons, and at best do not appear to be much worse
than diamond knives in regards to their initial sharpness.

Initially I had imagined that SiC knives might potentially replace
diamond knives, since the hardness of SiC crystals is 9+ on the Mho's
scale, while diamond is 10.

As it turns out, my SiC knives gradually become dull over the course
of making hundreds of sections. However they are distinctly more
durable than glass knives, and the lapped faces are much flatter and
the edges wider compared to glass.

The SiC knife durability seems to be a function of the hardness of
the embedding plastic being sectioned, as one may imagine. (They
section glycol methacrylate nicely, for making floating ribbons,
which is helpful since I intend to use the SiC knives myself for LM
work).

My question is how useful such knives are likely to be in practice? I
imagine SiC knives might fill a niche market for disposable
substitutes for diamond knives, when an inexpensive alternative to
glass knives is needed only occasionally and the purchase of a
diamond knife doesn't make sense, or maybe for student teaching.

It seems likely that the same technology can probably be used to make
sapphire knives of equivalent initial sharpness (sapphire is much
tougher and more fracture resistant than SiC but not so hard as SiC).
But I don't know, my efforts so far have only concerned SiC knives.

I expect to publish my SiC knife making technology and put it in the
public domain, but for now I am soliciting comments on their
potential usefulness. -- Roger, Austin, Tx







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From: luce-at-earthtech.org
Date: Sun, 17 Jul 2005 19:01:51 -0500
Subject: [Microscopy] AskAMicroscopist: schematics for an International Scientific

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Contact Art McCanna at MAS amccanna-at-mastest.com ; (770)866-3200;
direct-(770)866-3212. MAS is exclusive factory representative for GATAN ion
mills.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
X-from: {john.bonevich-at-nist.gov}
To: {vitalylazar-at-att.net}
Sent: Friday, July 15, 2005 1:13 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (luce-at-earthtech.org) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, July 17, 2005 at 16:06:51
---------------------------------------------------------------------------

Email: luce-at-earthtech.org
Name: George Luce

Organization: Earthtech Internaional, Inc.

Education: Graduate College

Location: Austin, Texas, USA

Question: We are looking for the electrical schematics for an International Scientific Instruments (ISI) Model 100B Scanning Electron Microscope. (~1979 vintage, probably made by Akashi)

Is this company still in business, or is there another resource for information about this SEM?

Thanks,

George Luce
luce-at-earthtech.org

Earthtech International Inc.
Austin, TX
www.earthtech.org



---------------------------------------------------------------------------

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From: pwje-at-sympatico.ca
Date: Sun, 17 Jul 2005 20:46:37 -0500
Subject: [Microscopy] Re: AskAMicroscopist: schematics for an International Scientific

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi George,
So you have a ISI 100B, I rep and service ISI SEMs in Canada but if I can be
of any help please ask.

To start with what is the problem you have?
I think I have the manual and schematics but they will cost you to get them
copied I'm afraid.

If you don't get any other offers let me know.
Cheers
Peter Earl
Toronto,Canada



==============================Original Headers==============================
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From: lcgould-at-med.cornell.edu
Date: Mon, 18 Jul 2005 09:11:25 -0500
Subject: [Microscopy] Re: Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roger

the only comment that I can make is that sapphire knives were marketed
a few (maybe 20) years ago but they were much more expensive than glass
and not as hard as diamond so were never really that popular. I suppose
in the real world a lot will depend on price and quality and whether
there is a sufficiently large market, now.

I wish you luck.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: rcbaker-at-eden.infohwy.com

Hi Roger,
It sounds interesting, keep us informed.
Years ago, Diatome did market a Sapphire knife as a less-expensive
alternative to diamonds. In my lab, they proved difficult to clean,
and did not wear well enough to continue using them. They seem to
have disappeared from the market, so my guess is that others had the
same observations.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: cammer-at-aecom.yu.edu
Date: Mon, 18 Jul 2005 09:14:33 -0500
Subject: [Microscopy] Re: vibrations in air cooled CCD cameras; follow-up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The problem has been solved by using external fans running through tubes to
the camera.

Roper and Cooke each have their own different designs for the fan. Each
were extremely helpful with the retrofits.

Cooke runs the camera power through the fan; this assures that the fan must
be on for the camera to run. Roper doesn't have this safety feature.

I would recommend not purchasing a camera with an on board fan. Always go
for external cooling.

Thanks for the help from everybody who responded to my query
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**


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From: murraytm-at-u.washington.edu
Date: Mon, 18 Jul 2005 10:56:23 -0500
Subject: [Microscopy] SEM Sample Prep Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a student who wants to look at clusters of methanosarcina and
methanosaeta in an aqueous solution. I have a materials background
and have never worked with this type of sample. The student found a
reference for preparing samples for electron microscopy which looks
to me like it is for TEM. She is interested in what the clusters
look like so is interested in retaining their relative position. I
think SEM would be a good technique to see the 3D cluster if there is
a method to prepare samples.

I have a FESEM which is high vacuum. I don't have easy access to an
environmental SEM, so I'm looking for a technique to image these
clusters in a high vacuum SEM.

Any suggestions for prep techniques and/or references?

Thanks,

Tom

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email:
murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195


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From: jchandler-at-ial-fa.com
Date: Mon, 18 Jul 2005 11:51:41 -0500
Subject: [Microscopy] Training: looking for TEM short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Try our new Quantomix Wet SEM Technology. Sounds like you have a perfect
application for the QX-102.

http://www.emsdiasum.com/microscopy/products/sem/wetsem.aspx?mm=11

Al Coritz, Technical Engineer
Electron Microscopy Sciences
www.emsdiasum.com


----- Original Message -----
X-from: {murraytm-at-u.washington.edu}
To: {Sampleprep-at-earthlink.net}
Sent: Monday, July 18, 2005 11:56 AM

A colleague of mine is looking for an intensive TEM short course, 3-4 days,
that covers theory and operation of TEM's, as well as interpretation of
images, particularly as they are used in materials science. The course at
Lehigh University for this year was just held, and waiting until next year
is not a good option.

If you teach or know about such a course, please contact me offline and I
will it forward the information.

Thanks very much,

--John Chandler
Fort Collins, CO
jchandler-at-ial-fa.com
970.217.1321


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From: peoshel-at-wisc.edu
Date: Mon, 18 Jul 2005 12:50:56 -0500
Subject: [Microscopy] Re: SEM Sample Prep Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom,

What's the procedure? Very likely, she can use it up through the 100%
ethanol steps, whether or not it is for TEM. The critters can be
filtered onto 0.22 micron membrane filters (the kind with nice, round
pores, not a Millipore), and the filter critical point dried.
An alternative to CPD is to air dry from HMDS (hexamethyldisilizane).
Process the samples in 1.5 mL minifuge tubes, not on filters. After
the last EtOH, go through a 2:1 1:1 1:2 EtOH:HMDS series, 3 changes
in 100% HMDS, put sputter coated filters on the SEM stubs, drop the
bugs in HMDS on the filters, and allow to air dry at room temp **do
all this in a fume hood!**.
See the U. Florida "tips and tricks" web site:
http://www.biotech.ufl.edu/EM/tips/index.html

Or have a chat with the U. Washington biological EM people.

Phil

} I have a student who wants to look at clusters of methanosarcina and
} methanosaeta in an aqueous solution. I have a materials background
} and have never worked with this type of sample. The student found a
} reference for preparing samples for electron microscopy which looks
} to me like it is for TEM. She is interested in what the clusters
} look like so is interested in retaining their relative position. I
} think SEM would be a good technique to see the 3D cluster if there is
} a method to prepare samples.
}
} I have a FESEM which is high vacuum. I don't have easy access to an
} environmental SEM, so I'm looking for a technique to image these
} clusters in a high vacuum SEM.
}
} Any suggestions for prep techniques and/or references?
}
} Thanks,
}
} Tom
}
} ------------------------------------------------------------------------
} ---------------------------------------------
} Thomas M Murray email:
} murraytm-at-u.washington.edu
} Electron Microscopy Center Manager Phone: (206)543-2836
} Materials Science & Engineering Fax: (206)543-3100
} Box 352120 302 Roberts Hall Cell: (425)345-0083
} University of Washington
} Seattle, WA 98195
}
}
} ==============================Original Headers==============================
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

==============================Original Headers==============================
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From: bozzola-at-siu.edu
Date: Mon, 18 Jul 2005 14:49:53 -0500
Subject: [Microscopy] Re: Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HI ROGER,

One potential use would be to section materials that might damage a
diamond knife, but that could not be cut using a glass knife. For
example, specimens containing potentially damaging inclusions as
sand, metals, etc. or to cut hard botanical specimens.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################

==============================Original Headers==============================
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From: JOHN.WHEATLEY-at-asu.edu
Date: Mon, 18 Jul 2005 14:59:32 -0500
Subject: [Microscopy] RE: Training: looking for TEM short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

We have a Winter School from January 9 through 12. One half day on the 13th. Please go to our web site where you will find a description of last year's school. We will have the info for 2006 up in a few days. Let me know if you need more info. John Wheatley


http://www.asu.edu/clas/csss/workshops/HREMschool.html



} ----------
} From: jchandler-at-ial-fa.com
} Reply To: microscopy-at-microscopy.com
} Sent: Monday, July 18, 2005 9:53 AM
} To: John Wheatley
} Subject: [Microscopy] Training: looking for TEM short course
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} A colleague of mine is looking for an intensive TEM short course, 3-4 days,
} that covers theory and operation of TEM's, as well as interpretation of
} images, particularly as they are used in materials science. The course at
} Lehigh University for this year was just held, and waiting until next year
} is not a good option.
}
} If you teach or know about such a course, please contact me offline and I
} will it forward the information.
}
} Thanks very much,
}
} --John Chandler
} Fort Collins, CO
} jchandler-at-ial-fa.com
} 970.217.1321
}
}
} ==============================Original Headers==============================
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From: TindallR-at-missouri.edu
Date: Mon, 18 Jul 2005 16:21:32 -0500
Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

Thanks so much for getting back to me on your training course. I had looked
at your website before polling the microscopy listserv, and it seemed to
have lots of what is needed. ASU is one of the few centers for EM that I
looked at before asking the question. I will pass along your information.

With best regards,

--John | jchandler-at-ial-fa.com | 970.217.1321

----- Original Message -----
X-from: {JOHN.WHEATLEY-at-asu.edu}
To: {jchandler-at-ial-fa.com}
Sent: Monday, July 18, 2005 1:59 PM

Dear Listers,

Below is a compilation of the replies I received to my recent question
about Cu vs. Ni grids for immunolabeling. I usually don't do this
without express permission, but most everybody posted to the list
anyway, and I made sure to edit those that didn't. If it bothers
anyone, let me know and I will never do it again.

In summary, it appears that copper can and will react with salts in
buffers and other solutions, although some people still have decent
luck. This problem can be ameliorated by using coated Cu grids and only
wetting the coated side and by shortening incubation times

Also, Marc Pypaert had the interesting suggestion of just using Ni grids
for everything, since they're not much more expensive, thereby avoiding
the problem entirely. Any thoughts on this?

Many thanks to everyone who replied! I now have a much better response
to our clients' questions on this matter (not to mention my own).

Cheers,
Randy


Over the years I have frequently seen cases where the samples themselves
have reacted with the copper grid. Two examples:

1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
precipitates were ugly and analyzed as copper sulphide. Our surmise was
that the iron and copper had undergone ion exchange while the grid (and
carbon film) was wet with the culture medium. When we used nickel
grids, the precipitates were well formed and composed of iron and
sulphur.

2) Looking at Si precipitates in Al-Si electronic bond wire. The
specimens were prepared by embedding and microtoming, floating in DI
water. The Si precipitates were surrounded by an ugly mess containing
masses of copper. Again, presumed to me electrochemical reaction
between the Al and the Cu grid, through the DI water medium, somehow
preferentially at the Si precipitates. Again, use of Ni grids produced
good pictures allowing us to characterize the precipitation.

Having said all that, we continue to default to use copper grids!

Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA


-------------------------------------------

The main problem encountered with copper grids used for
immunocytochemistry or immunogold labeling is the reaction of the copper
with salts in the buffers used during labeling. This is a time-related
reaction, and can usually be avoided by having short labeling runs and
using grids with films on them. Gilder grids, available from major
microscope supply vendors, are gold-coated copper grids, and are not
that much more expensive than regular copper grids. These grids may be
the variety used by researchers who have reported having no problems
with their grids during their immuno runs.
I usually use nickel grids for my work, but have used
formvar-coated copper grids without problems for 1 day immuno runs so
long as the film is intact and as long as I don't get clumsy and end up
sinking my grids in my solutions (if I do sink them, I do a quick
distilled water rinse, blot the back of the grids with filter paper
until almost dry, then re-float them where I left off). As others have
shared, the nickel grids are much sturdier, not too expensive, and are
not a problem to handle as long as you use antimagnetic forceps. With
the nickel grids, remember to correct for astigmatism in the TEM by
using a hole in your sample on the nickel grid. This will accommodate
for any inherent residual magnetic field in the grid itself.

Edward Haller
Lab Manager, Diagnostic Electron Microscopy Lab University of South
Florida Pathology Department Tampa, FL 33612

------------------------------------------------------------

We have had problems with Cu grids, even Formvar coated ones. I have
precoated copper grids with Parlodion by dipping, blotting and drying
before use, with or with out a formvar coating. THat method seems to
protect the copper from reacting with PBS or whatever. This is not
original to me, but I cannot recall where I read this (among many other
things I can no longer recall). I think it might have been one of those
smart Swiss guys.

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program Scientific Director, Electron
Microscopy P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
-------------------------------------------------------------------

I had a bad experience with formvar-copper grids in immunogold
experiment many years ago when incubating primary antibody overnight and
the rest done on the following morning. I am using Nickel grids now --
no more problems.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
------------------------------------------------------------------------
------------

recently i did a long ultracentrifuge run to load some 10S particles
onto a formvar-copper grid. the grids reacted with the phosphate buffer
over the 4 hour spin time. not a pretty picture - i had to go back to
my collaborator and get fresh material so we could put it onto
formvar-nickel grids. wonderful gentleman.


Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
----------------------------------------------------------------

We used Cu grids for many years. Normally we use formvar + carbon
coated grids for ICC since we have a lot less loss of sections. We also
tend to do long incubations ...usually overnight. This is time
efficient and also lets us use highly diluted antibody so also minimize
background due to cross-reactions from contaminants in polyclonal
antibodies.

Occasionally we would have a reaction due to copper oxidizing with
resultant green solution. I attributed this to salts or traces of Tween
20 in the buffer and breaks in the coating exposing the Cu. As far as I
can see, this is the only reason for not using Cu grids if you use
coated grids. We have switched for the most part to using coated Ni
grids to avoid this problem.

On the other hand, occasionally we need to do a double labeling using
uncoated grids and both sides of the sections. I would hesitate to use
Cu for this and prefer Ni. Gold would work but is both more expensive
and more delicate than Ni grids.

Debby Sherman, Manager
Life Science Microscopy Facility
Purdue University
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
------------------------------------------------------------------------
-

X-from my experience, coated copper grids will be fine for most (if not
all) immunolabelling, providing the solutions do not wet the uncoated
side of the grid. Uncoated copper grids always have been fine when I've
used PBS.

However, copper grids (uncoated - or the uncoated side) usually will
react with Tris-HCl buffers and some of the high salt buffer
formulations that I've used. Longer incubation times (eg. overnight)
cause more reaction with the grid than shorter times (eg. 60mins) - so
you may be able to use copper grids without problems if incubations are
short, even with buffer formulations that might react with the grid if
left for longer periods. It also is more difficult to prevent wetting
of the reverse (uncoated) side of coated copper grids if incubation
times are long, and particularly if wetting agents are used in the
buffer formulation.

When I started immunolabelling in the early 1980s - and hadn't yet heard
about the use of nickel grids - I used uncoated copper grids (and PBS)
for years without problems. My current lab generally uses a high salt
Tris-HCl buffer which includes a small amount of Tween 20, so we
routinely use nickel grids for immunolabelling. Nickel grids do have
some disadvantages (eg. charge), but we run a multi-user facility, often
training students or other inexperienced users, we've found nickel the
best option to overcome potential "grid-reaction" problems. However,
nickel grids certainly aren't essential for successful immunolabelling -
you just need to be aware of the possible problems.

Dr Deborah Stenzel
Analytical Electron Microscopy Facility
Queensland University of Technology
GPO Box 2434
Brisbane 4001
Australia

------------------------------------------------

I know (from sad experience) that Tris buffers will etch Cu grids - the
antibody droplets turn a lovely blue and there is gunk all over the
sections. I use Ni grids, only because I prefer Tris buffers (no real
reason why, I guess) for IEM and my current PI cringes at buying Au
grids.
I know one person who does all of her labelling in phosphate buffer and
uses Cu grids with no precipitate problems.

As for the other reasons you Googled....I've heard them, but never seen
any documentation nor had personal experience.

Can't wait to hear if anyone has actual evidence for some of the other
no-Cu reasons!

Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
----------------------------------------------------------------

Hi Randy,
I had problems with Cu grids when incubating sections for long periods -
overnight for instance. The copper would react with whatever was
available and form green-blue salts. Not unexpected. We switched to
nickel grids for a while but they charge, gold but they are
delicate...by this time we had shortened the incubation times used, and
tried copper again... And had no problem using coated grids and
incubation times of an hour or so. Grids are floated on drops of media
so that only the coated side is wetted - I don't know if that is
important.

Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C


I only ran into the "use only Ni or Au grids" rule here, in my current
job, and my predecessor certainly produced some superb images showing
gold labelling of TEM sections. Blissfully unaware of this rule, I had
been using copper grids for all EM immunolabelling. To get good
labelling of one particular structure, which is about 40 nm diameter, I
used uncoated thin-bar Cu grids so I could get labelling on both sides
of the section - seemed to work just fine. I'll be interested to see
the responses to your question.

Dr. Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia








Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: sryazant-at-ucla.edu
Date: Mon, 18 Jul 2005 17:44:32 -0500
Subject: [Microscopy] Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Roger
I am not sure is tungsten carbide is similar to SiC or not. My experience
with tungsten carbide basically was negative - this knife starts scratching
the surface after just 50+ 0.5 um sections of standard plastic embedded
tissue (no hard inclusions etc). As manufacturer explained to me it's
because of polycrystalline nature of the material - small crystals just
became loose and left the edge creating ruff surface. From this point of
view, amorphous (like glass) or monocrystal (like diamond) material is
preferable for good sections. Sapphire (hardness is 9) knifes were on the
market for while without great success. From economical point of view,
tungsten carbide knifes were also not so good - $100 each with ability to
produce only 50+ good sections. If SiC acts in the similar way, then it
would be difficult to use it in EM applications. Another aspect of using
exotic materials - is it hydrophilic or hydrophobic? Could you use water
with them? How easy to clean it up and handle? Sergey

At 07:00 AM 7/18/2005, you wrote:



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From: rcbaker-at-eden.infohwy.com
Date: Mon, 18 Jul 2005 21:18:00 -0500
Subject: [Microscopy] Re: Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Jul 18, 2005, at 5:48 PM, sryazant-at-ucla.edu wrote:
}
} Dear Roger
} I am not sure is tungsten carbide is similar to SiC or not. My
} experience
} with tungsten carbide basically was negative - this knife starts
} scratching
} the surface after just 50+ 0.5 um sections of standard plastic
} embedded
} tissue (no hard inclusions etc). As manufacturer explained to me it's
} because of polycrystalline nature of the material - small crystals
} just
} became loose and left the edge creating ruff surface. From this
} point of
} view, amorphous (like glass) or monocrystal (like diamond) material is
} preferable for good sections. Sapphire (hardness is 9) knifes were
} on the
} market for while without great success. From economical point of
} view,
} tungsten carbide knifes were also not so good - $100 each with
} ability to
} produce only 50+ good sections. If SiC acts in the similar way,
} then it
} would be difficult to use it in EM applications. Another aspect of
} using
} exotic materials - is it hydrophilic or hydrophobic? Could you use
} water
} with them? How easy to clean it up and handle? Sergey



Thanks everyone, I've gotten lots of helpful advice and comments, so
I'll try to sum up my conclusions.

Some background: Silicon carbide (SiC) is produced by a very old
company Washington Mills located near Niagara Falls but production is
in Illinois. Blessed folks; they sent me a sample of crystals for free.

{http://www.medibix.com/company.jsp?company_id=10001791}

It is made in tonnage quantities as clusters of glossy flat hexagonal
black crystals up to a few centimeters across, and which are actually
deep transparent blue and are crushed for abrasives and metallurgy.
The cost of this industrial crystalline material is negligible. The
Cree Corporation in NC makes also clear white SiC crystals and wafers
for a fairly high price, but only sells them already faceted into
clear diamond substitutes for jewelry. Such clear SiC crystals are
termed "moissenite".

SiC crystals are very hard and brittle and the edge breaks when you
try to sharpen them into knives using anything resembling normal gem
faceting methods. But knives can be made using the proper lapping
technique and the appropriate grades of diamond powder. Here is an
interesting link on modern diamond powder technology:

{http://www.ceramicindustry.com/CDA/ArticleInformation/features/
BNP__Features__Item/0,2710,152388,00.html}

At any rate, besides glass, only hard single crystals of diamond,
sapphire, and silicon carbide seem to be appropriate for making
ultramicrotome knives, which by their nature demand a perfectly
homogeneous material. Sapphire knives were sold at one time by
Diatome but the price was reputedly about $500 apiece and they became
dull, unlike diamond knives, so the economics was questionable.

My SiC knives become dull after a few hundred slices, probably
depending on the the hardness of the embedding plastic (glycol
methacrylate works well), but the raw materials are inexpensive and
my lapping machine only cost about $50 to build, so the labor of
making them is inherently the major cost.

Since SiC crystals are difficult to sharpen without breaking the
edge, I assume the exact same lapping techniques would also be
appropriate for sapphire, which is softer tougher and less brittle
than SiC; the latter are the very devil to sharpen without breaking.
I learned how to make them through sheer stubbornness in order to
make many serial sections with my Porter Blum MT-2 without paying
$1000+ for a diamond knife.

I can only guess at the durability of sapphire knives, but the fact
that they were once offered indicates that they might still be a
viable alternative for some applications if they were only available
at a lower cost.

My SiC crystals are epoxyed in a slot cut in the apex of a steel
holder the same size and shape as a right angle glass knife. The
clean crystals are hydrophilic (I assume the exposed surface layer at
the atomic level is silicon oxide). I clean them by wiping the edge
sideways with Teflon and don't have many wetting problems.

My conclusion is that I don't much want to go the trouble of trying
to make money patenting, licensing and selling knives, but that I
should publish my technique of making them, most likely in the Review
of Scientific Instruments, and see if the world pays attention. I
don't think the method of sharpening hard crystals to give very sharp
edges has ever been published; I can't find it in the scientific
literature. I seriously doubt it would work for diamonds without
modification.

The only possible drawback to open publication is that no one maker
could make a lot of money making them, and thus might not offer them,
assuming they prove to be a modestly useful alternative to diamonds.

I assume that maybe the Chinese could sell disposable knives for $20
or so and they might catch on for student use and low budget and
occasional use. Glass knives are clearly sharper for a few dozen
sections and diamond knives clearly last longer.

-- Roger Baker Jr.
1303 Bentwood,
Austin Tx, 78722












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From: jtwilley-at-sprynet.com
Date: Mon, 18 Jul 2005 22:24:33 -0500
Subject: [Microscopy] Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Roger,

You should be aware that recently, gem-quality silicon carbide has been under production and is
being marketed under its mineralogical name as "synthetic moissanite". This grade is significantly
more free of defects and might yield a much more durable edge. I don't know the economics of
producing a knife edge from this stock but I suspect that the material price is not prohibitively
high, given the prices of stones that are being sold as jewelry and the usual mark-up in that
field.

Sincerely,

John Twilley

rcbaker-at-eden.infohwy.com wrote:

} Thanks everyone, I've gotten lots of helpful advice and comments, so
} I'll try to sum up my conclusions.
}
} Some background: Silicon carbide (SiC) is produced by a very old
} company Washington Mills located near Niagara Falls but production is
} in Illinois. Blessed folks; they sent me a sample of crystals for free.
}
} {http://www.medibix.com/company.jsp?company_id=10001791}
}
} It is made in tonnage quantities as clusters of glossy flat hexagonal
} black crystals up to a few centimeters across, and which are actually
} deep transparent blue and are crushed for abrasives and metallurgy.
} The cost of this industrial crystalline material is negligible. The
} Cree Corporation in NC makes also clear white SiC crystals and wafers
} for a fairly high price, but only sells them already faceted into
} clear diamond substitutes for jewelry. Such clear SiC crystals are
} termed "moissenite".
}
} SiC crystals are very hard and brittle and the edge breaks when you
} try to sharpen them into knives using anything resembling normal gem
} faceting methods. But knives can be made using the proper lapping
} technique and the appropriate grades of diamond powder. Here is an
} interesting link on modern diamond powder technology:
}
} {http://www.ceramicindustry.com/CDA/ArticleInformation/features/
} BNP__Features__Item/0,2710,152388,00.html}
}
} At any rate, besides glass, only hard single crystals of diamond,
} sapphire, and silicon carbide seem to be appropriate for making
} ultramicrotome knives, which by their nature demand a perfectly
} homogeneous material. Sapphire knives were sold at one time by
} Diatome but the price was reputedly about $500 apiece and they became
} dull, unlike diamond knives, so the economics was questionable.
}
} My SiC knives become dull after a few hundred slices, probably
} depending on the the hardness of the embedding plastic (glycol
} methacrylate works well), but the raw materials are inexpensive and
} my lapping machine only cost about $50 to build, so the labor of
} making them is inherently the major cost.
}
} Since SiC crystals are difficult to sharpen without breaking the
} edge, I assume the exact same lapping techniques would also be
} appropriate for sapphire, which is softer tougher and less brittle
} than SiC; the latter are the very devil to sharpen without breaking.
} I learned how to make them through sheer stubbornness in order to
} make many serial sections with my Porter Blum MT-2 without paying
} $1000+ for a diamond knife.
}
} I can only guess at the durability of sapphire knives, but the fact
} that they were once offered indicates that they might still be a
} viable alternative for some applications if they were only available
} at a lower cost.
}
} My SiC crystals are epoxyed in a slot cut in the apex of a steel
} holder the same size and shape as a right angle glass knife. The
} clean crystals are hydrophilic (I assume the exposed surface layer at
} the atomic level is silicon oxide). I clean them by wiping the edge
} sideways with Teflon and don't have many wetting problems.
}
} My conclusion is that I don't much want to go the trouble of trying
} to make money patenting, licensing and selling knives, but that I
} should publish my technique of making them, most likely in the Review
} of Scientific Instruments, and see if the world pays attention. I
} don't think the method of sharpening hard crystals to give very sharp
} edges has ever been published; I can't find it in the scientific
} literature. I seriously doubt it would work for diamonds without
} modification.
}
} The only possible drawback to open publication is that no one maker
} could make a lot of money making them, and thus might not offer them,
} assuming they prove to be a modestly useful alternative to diamonds.
}
} I assume that maybe the Chinese could sell disposable knives for $20
} or so and they might catch on for student use and low budget and
} occasional use. Glass knives are clearly sharper for a few dozen
} sections and diamond knives clearly last longer.
}
} -- Roger Baker Jr.
} 1303 Bentwood,
} Austin Tx, 78722
}
}
} ==============================Original Headers==============================
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From: sousan.abolhassani-at-psi.ch
Date: Tue, 19 Jul 2005 01:34:57 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: "Amit Kohn" {amit.kohn-at-materials.oxford.ac.uk

Dear Randy,

I followed this thread by curiosity as I knew
that copper is not always a good solution.
And it was good to receive your message so
that I know that there was no response that
we missed.
I think that the idea of giving a summary
of the answers to the list, is a very
respectful manner to contribute to its
usefulness.
Thanks a lot,

Sousan

TindallR-at-missouri.edu wrote:
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} Below is a compilation of the replies I received to my recent question
} about Cu vs. Ni grids for immunolabeling. I usually don't do this
} without express permission, but most everybody posted to the list
} anyway, and I made sure to edit those that didn't. If it bothers
} anyone, let me know and I will never do it again.
}
} In summary, it appears that copper can and will react with salts in
} buffers and other solutions, although some people still have decent
} luck. This problem can be ameliorated by using coated Cu grids and only
} wetting the coated side and by shortening incubation times
}
} Also, Marc Pypaert had the interesting suggestion of just using Ni grids
} for everything, since they're not much more expensive, thereby avoiding
} the problem entirely. Any thoughts on this?
}
} Many thanks to everyone who replied! I now have a much better response
} to our clients' questions on this matter (not to mention my own).
}
} Cheers,
} Randy
}
}
} Over the years I have frequently seen cases where the samples themselves
} have reacted with the copper grid. Two examples:
}
} 1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
} precipitates were ugly and analyzed as copper sulphide. Our surmise was
} that the iron and copper had undergone ion exchange while the grid (and
} carbon film) was wet with the culture medium. When we used nickel
} grids, the precipitates were well formed and composed of iron and
} sulphur.
}
} 2) Looking at Si precipitates in Al-Si electronic bond wire. The
} specimens were prepared by embedding and microtoming, floating in DI
} water. The Si precipitates were surrounded by an ugly mess containing
} masses of copper. Again, presumed to me electrochemical reaction
} between the Al and the Cu grid, through the DI water medium, somehow
} preferentially at the Si precipitates. Again, use of Ni grids produced
} good pictures allowing us to characterize the precipitation.
}
} Having said all that, we continue to default to use copper grids!
}
} Anthony J. Garratt-Reed, M.A., D.Phil.
} MIT Room 13-1027
} 77 Massachusetts Avenue
} Cambridge, MA 02139-4307
} USA
}
}
} -------------------------------------------
}
} The main problem encountered with copper grids used for
} immunocytochemistry or immunogold labeling is the reaction of the copper
} with salts in the buffers used during labeling. This is a time-related
} reaction, and can usually be avoided by having short labeling runs and
} using grids with films on them. Gilder grids, available from major
} microscope supply vendors, are gold-coated copper grids, and are not
} that much more expensive than regular copper grids. These grids may be
} the variety used by researchers who have reported having no problems
} with their grids during their immuno runs.
} I usually use nickel grids for my work, but have used
} formvar-coated copper grids without problems for 1 day immuno runs so
} long as the film is intact and as long as I don't get clumsy and end up
} sinking my grids in my solutions (if I do sink them, I do a quick
} distilled water rinse, blot the back of the grids with filter paper
} until almost dry, then re-float them where I left off). As others have
} shared, the nickel grids are much sturdier, not too expensive, and are
} not a problem to handle as long as you use antimagnetic forceps. With
} the nickel grids, remember to correct for astigmatism in the TEM by
} using a hole in your sample on the nickel grid. This will accommodate
} for any inherent residual magnetic field in the grid itself.
}
} Edward Haller
} Lab Manager, Diagnostic Electron Microscopy Lab University of South
} Florida Pathology Department Tampa, FL 33612
}
} ------------------------------------------------------------
}
} We have had problems with Cu grids, even Formvar coated ones. I have
} precoated copper grids with Parlodion by dipping, blotting and drying
} before use, with or with out a formvar coating. THat method seems to
} protect the copper from reacting with PBS or whatever. This is not
} original to me, but I cannot recall where I read this (among many other
} things I can no longer recall). I think it might have been one of those
} smart Swiss guys.
}
} Gregory W. Erdos, Ph.D.
} Assistant Director, Biotechnology Program Scientific Director, Electron
} Microscopy P.O. Box 118525
} 217 Carr Hall
} University of Florida
} Gainesville, FL 32611
} -------------------------------------------------------------------
}
} I had a bad experience with formvar-copper grids in immunogold
} experiment many years ago when incubating primary antibody overnight and
} the rest done on the following morning. I am using Nickel grids now --
} no more problems.
}
} Ann Fook Yang
} EM Unit/ Unite EM
} Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
} 960 Carling Ave/960 Boul Carling
} Ottawa,Ontario/Ottawa, Ontario
} Canada
} K1A 0C6
} ------------------------------------------------------------------------
} ------------
}
} recently i did a long ultracentrifuge run to load some 10S particles
} onto a formvar-copper grid. the grids reacted with the phosphate buffer
} over the 4 hour spin time. not a pretty picture - i had to go back to
} my collaborator and get fresh material so we could put it onto
} formvar-nickel grids. wonderful gentleman.
}
}
} Paul R. Hazelton, PhD
} Electron Microscope Unit
} University of Manitoba
} Department of Medical Microbiology
} 531 Basic Medical Sciences Building
} 730 William Avenue
} Winnipeg, Manitoba, Canada, R3E 0W3
} ----------------------------------------------------------------
}
} We used Cu grids for many years. Normally we use formvar + carbon
} coated grids for ICC since we have a lot less loss of sections. We also
} tend to do long incubations ...usually overnight. This is time
} efficient and also lets us use highly diluted antibody so also minimize
} background due to cross-reactions from contaminants in polyclonal
} antibodies.
}
} Occasionally we would have a reaction due to copper oxidizing with
} resultant green solution. I attributed this to salts or traces of Tween
} 20 in the buffer and breaks in the coating exposing the Cu. As far as I
} can see, this is the only reason for not using Cu grids if you use
} coated grids. We have switched for the most part to using coated Ni
} grids to avoid this problem.
}
} On the other hand, occasionally we need to do a double labeling using
} uncoated grids and both sides of the sections. I would hesitate to use
} Cu for this and prefer Ni. Gold would work but is both more expensive
} and more delicate than Ni grids.
}
} Debby Sherman, Manager
} Life Science Microscopy Facility
} Purdue University
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} ------------------------------------------------------------------------
} -
}
} X-from my experience, coated copper grids will be fine for most (if not
} all) immunolabelling, providing the solutions do not wet the uncoated
} side of the grid. Uncoated copper grids always have been fine when I've
} used PBS.
}
} However, copper grids (uncoated - or the uncoated side) usually will
} react with Tris-HCl buffers and some of the high salt buffer
} formulations that I've used. Longer incubation times (eg. overnight)
} cause more reaction with the grid than shorter times (eg. 60mins) - so
} you may be able to use copper grids without problems if incubations are
} short, even with buffer formulations that might react with the grid if
} left for longer periods. It also is more difficult to prevent wetting
} of the reverse (uncoated) side of coated copper grids if incubation
} times are long, and particularly if wetting agents are used in the
} buffer formulation.
}
} When I started immunolabelling in the early 1980s - and hadn't yet heard
} about the use of nickel grids - I used uncoated copper grids (and PBS)
} for years without problems. My current lab generally uses a high salt
} Tris-HCl buffer which includes a small amount of Tween 20, so we
} routinely use nickel grids for immunolabelling. Nickel grids do have
} some disadvantages (eg. charge), but we run a multi-user facility, often
} training students or other inexperienced users, we've found nickel the
} best option to overcome potential "grid-reaction" problems. However,
} nickel grids certainly aren't essential for successful immunolabelling -
} you just need to be aware of the possible problems.
}
} Dr Deborah Stenzel
} Analytical Electron Microscopy Facility
} Queensland University of Technology
} GPO Box 2434
} Brisbane 4001
} Australia
}
} ------------------------------------------------
}
} I know (from sad experience) that Tris buffers will etch Cu grids - the
} antibody droplets turn a lovely blue and there is gunk all over the
} sections. I use Ni grids, only because I prefer Tris buffers (no real
} reason why, I guess) for IEM and my current PI cringes at buying Au
} grids.
} I know one person who does all of her labelling in phosphate buffer and
} uses Cu grids with no precipitate problems.
}
} As for the other reasons you Googled....I've heard them, but never seen
} any documentation nor had personal experience.
}
} Can't wait to hear if anyone has actual evidence for some of the other
} no-Cu reasons!
}
} Tamara Howard
} Department of Cell Biology and Physiology
} University of New Mexico - Health Sciences Center
} Albuquerque, NM 87131
} ----------------------------------------------------------------
}
} Hi Randy,
} I had problems with Cu grids when incubating sections for long periods -
} overnight for instance. The copper would react with whatever was
} available and form green-blue salts. Not unexpected. We switched to
} nickel grids for a while but they charge, gold but they are
} delicate...by this time we had shortened the incubation times used, and
} tried copper again... And had no problem using coated grids and
} incubation times of an hour or so. Grids are floated on drops of media
} so that only the coated side is wetted - I don't know if that is
} important.
}
} Dr SJ Stowe
} Facility Coordinator
} ANU Electron Microscopy Unit
} ANU CRICOS#00120C
}
}
} I only ran into the "use only Ni or Au grids" rule here, in my current
} job, and my predecessor certainly produced some superb images showing
} gold labelling of TEM sections. Blissfully unaware of this rule, I had
} been using copper grids for all EM immunolabelling. To get good
} labelling of one particular structure, which is about 40 nm diameter, I
} used uncoated thin-bar Cu grids so I could get labelling on both sides
} of the section - seemed to work just fine. I'll be interested to see
} the responses to your question.
}
} Dr. Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
}
}
}
}
}
}
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original Headers==============================
} 54, 25 -- From TindallR-at-missouri.edu Mon Jul 18 16:21:32 2005
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5, 20 -- From sousan.abolhassani-at-psi.ch Tue Jul 19 01:34:57 2005
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From: scott.coutts-at-med.monash.edu.au
Date: Tue, 19 Jul 2005 01:46:02 -0500
Subject: [Microscopy] Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

I need to prep some samples for SEM, but I'm not sure of the best way to
go about it.

We have some bacteria that normally grow in a type of liquid nutrient
medium as free, discrete and seperate motile cells. However, we have
come across a strain that forms into 'mats' on the bottom of the culture
vessel. It is these that we would like to examine by SEM.

The problem is that the 'mats' are not like a normal biofilm in that
they are not strongly adherant to a solid surface, and the mat is not
very robust. The mats will 'float' off the bottom of the culture vessel
if they are disturbed and they will fragment into small flakes if the
vessel is shaken or swirled. The fragments are usually a milimetre or so
square.

Of course, the 'flakes' need to be flat in order to view them properly.
I thought I might be able to pipette them, in a drop of the media
they're already in, onto nucleopore membranes and allow them to settle,
then try to remove the rest of the media and hope that they stick
through the dehydration process... but i'm not sure they will. I'm
planning on using HMDS.

I'm goig to try it just to see what happens... but has anyone prepared a
sample like this that could suggest something that is know to work well?

Cheers,

--
Scott J. Coutts
---------------------------------------------------------------
Bacterial Pathogenesis Research Group
Department of Microbiology,
Box 53, Monash University, 3800, Australia
Phone: 9905 4838 Fax: 9905 4811
---------------------------------------------------------------

==============================Original Headers==============================
8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005
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From: Rosemary.White-at-csiro.au
Date: Tue, 19 Jul 2005 01:52:08 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Scott,
Is there a specific reason for looking at these bacteria with SEM?
cheers,
Roseamry

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


} From: scott.coutts-at-med.monash.edu.au
} Reply-To: microscopy-at-microscopy.com
} Date: Tue, 19 Jul 2005 01:47:57 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] Another SEM sample prep question
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi All,
}
} I need to prep some samples for SEM, but I'm not sure of the best way to
} go about it.
}
} We have some bacteria that normally grow in a type of liquid nutrient
} medium as free, discrete and seperate motile cells. However, we have
} come across a strain that forms into 'mats' on the bottom of the culture
} vessel. It is these that we would like to examine by SEM.
}
} The problem is that the 'mats' are not like a normal biofilm in that
} they are not strongly adherant to a solid surface, and the mat is not
} very robust. The mats will 'float' off the bottom of the culture vessel
} if they are disturbed and they will fragment into small flakes if the
} vessel is shaken or swirled. The fragments are usually a milimetre or so
} square.
}
} Of course, the 'flakes' need to be flat in order to view them properly.
} I thought I might be able to pipette them, in a drop of the media
} they're already in, onto nucleopore membranes and allow them to settle,
} then try to remove the rest of the media and hope that they stick
} through the dehydration process... but i'm not sure they will. I'm
} planning on using HMDS.
}
} I'm goig to try it just to see what happens... but has anyone prepared a
} sample like this that could suggest something that is know to work well?
}
} Cheers,
}
} --
} Scott J. Coutts
} ---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
} ---------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005
} 8, 17 -- Received: from omta04sl.mx.bigpond.com (omta04sl.mx.bigpond.com
} [144.140.93.156])
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} +0000
} 8, 17 -- Message-ID: {42DCA1AB.5070904-at-med.monash.edu.au}
} 8, 17 -- Date: Tue, 19 Jul 2005 16:46:03 +1000
} 8, 17 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au}
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==============================Original Headers==============================
4, 23 -- From Rosemary.White-at-csiro.au Tue Jul 19 01:52:08 2005
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From: K.venner-at-ion.ucl.ac.uk
Date: Tue, 19 Jul 2005 08:24:59 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: digital ccd cameras versus digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

IMAQUE IMAGING INC CALL
571-437-6593
----- Original Message -----
X-from: {luce-at-earthtech.org}
To: {JSMIT51-at-TAMPABAY.RR.COM}
Sent: Sunday, July 17, 2005 7:05 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on
Tuesday, July 19, 2005 at 04:02:00
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: kerrie

Organization: Institute of Neurology, University College London UK

Title-Subject: [Filtered] MListserver: digital ccd cameras versus digital plate system for TEM

Question: Many thanks to everyone who took the time and kindly contributed to the very long debate my query sparked up. All contributions have been compiled and have enabled me to present a balanced working view of the systems currently available.

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 13 -- From zaluzec-at-microscopy.com Tue Jul 19 08:24:59 2005
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6, 13 -- plate system for TEM
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From: hoffpajo-at-yahoo.com
Date: Tue, 19 Jul 2005 08:40:16 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

there is a very simple way to look at bacteria in the
SEM. just puch teh bacteria/liquid through a .45
micron filter. then process the filter paper for the
SEM. this is a tried and true method.
john

--- scott.coutts-at-med.monash.edu.au wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Hi All,
}
} I need to prep some samples for SEM, but I'm not
} sure of the best way to
} go about it.
}
} We have some bacteria that normally grow in a type
} of liquid nutrient
} medium as free, discrete and seperate motile cells.
} However, we have
} come across a strain that forms into 'mats' on the
} bottom of the culture
} vessel. It is these that we would like to examine by
} SEM.
}
} The problem is that the 'mats' are not like a normal
} biofilm in that
} they are not strongly adherant to a solid surface,
} and the mat is not
} very robust. The mats will 'float' off the bottom of
} the culture vessel
} if they are disturbed and they will fragment into
} small flakes if the
} vessel is shaken or swirled. The fragments are
} usually a milimetre or so
} square.
}
} Of course, the 'flakes' need to be flat in order to
} view them properly.
} I thought I might be able to pipette them, in a drop
} of the media
} they're already in, onto nucleopore membranes and
} allow them to settle,
} then try to remove the rest of the media and hope
} that they stick
} through the dehydration process... but i'm not sure
} they will. I'm
} planning on using HMDS.
}
} I'm goig to try it just to see what happens... but
} has anyone prepared a
} sample like this that could suggest something that
} is know to work well?
}
} Cheers,
}
} --
} Scott J. Coutts
}
---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
}
---------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul
} 19 01:46:02 2005
} 8, 17 -- Received: from omta04sl.mx.bigpond.com
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} {42DCA1AB.5070904-at-med.monash.edu.au}
} 8, 17 -- Date: Tue, 19 Jul 2005 16:46:03 +1000
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} {scott.coutts-at-med.monash.edu.au}
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From: peoshel-at-wisc.edu
Date: Tue, 19 Jul 2005 09:11:41 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
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Scott,

Interesting problem. The "matts" you refer to are likely to fragment
or worse if you use the usual filter-based preparation methods .
First question: do you have access to cryoSEM? If yes, this would be
the best way to do your samples. Freeze in a high-pressure freezer,
if available, or by plunging into slush nitrogen, then do the cryo
SEM. This is the method most likely to give you the unaltered
structure of the matts and the critters therein.
If you don't have access to cryoSEM, then the best way is to use
minifuge tubes or the like. Let the samples sit in the tube in fix,
then the EtOH dehydration steps, being very careful when you withdraw
the fluid to change it. Add the fluid for the next step as you
withdraw the old fluid to minimize disturbance of the matts, and
maybe add the fluid for each succeeding step down the side of the
tube.
I suggest a 2:1 1:1 1:2 EtOH:HMDS series before the 3 changes in 100% HMDS.
I've found it helpful to deposit the last HMDS + sample drops on a
sputter-coated membrane filter* stuck to a SEM stub for drying. This
further minimizes handling.
* 0.22 micron "nucleopore" type -- the ones with the nice, round
holes, not the torturous-path type of filter. Sputter coat both sides
of the filter before sticking to the stub, best, or stick to the stub
with conductive carbon tabs, then sputter coat. Then drop on the
samples in HMDS. (And yes, sputter coat for viewing in the SEM.)

Phil

} Hi All,
}
} I need to prep some samples for SEM, but I'm not sure of the best way to
} go about it.
}
} We have some bacteria that normally grow in a type of liquid nutrient
} medium as free, discrete and seperate motile cells. However, we have
} come across a strain that forms into 'mats' on the bottom of the culture
} vessel. It is these that we would like to examine by SEM.
}
} The problem is that the 'mats' are not like a normal biofilm in that
} they are not strongly adherant to a solid surface, and the mat is not
} very robust. The mats will 'float' off the bottom of the culture vessel
} if they are disturbed and they will fragment into small flakes if the
} vessel is shaken or swirled. The fragments are usually a milimetre or so
} square.
}
} Of course, the 'flakes' need to be flat in order to view them properly.
} I thought I might be able to pipette them, in a drop of the media
} they're already in, onto nucleopore membranes and allow them to settle,
} then try to remove the rest of the media and hope that they stick
} through the dehydration process... but i'm not sure they will. I'm
} planning on using HMDS.
}
} I'm goig to try it just to see what happens... but has anyone prepared a
} sample like this that could suggest something that is know to work well?
}
} Cheers,
}
} --
} Scott J. Coutts
} ---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
} ---------------------------------------------------------------
}
} ==============================Original Headers==============================
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

==============================Original Headers==============================
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From: tantt-at-umich.edu
Date: Tue, 19 Jul 2005 09:13:30 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: Looking T-Tool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tantt-at-umich.edu)
from http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, July 19, 2005 at 09:03:46
---------------------------------------------------------------------------

Email: tantt-at-umich.edu
Name: Tong Tat

Organization: University of Michigan

Title-Subject: [Filtered] MListserver: Looking T-Tool

Question: Hi, does anyone know where i can purchase T-Tool Jig for TEM sample preperation?

Model No: 510A & 510B.

Its by T&T Group.

Thanks

Tongtat

---------------------------------------------------------------------------

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From: mcauliff-at-umdnj.edu
Date: Tue, 19 Jul 2005 09:32:30 -0500
Subject: [Microscopy] dye sub, ink jet etc for prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John et al.:

A few more details on what I wrote on this subject before. Lyson has
been making inks and papers for photo quality ink jet printing for some
time. Their new system is the "Daylight Darkroom". It is a dedicated 4
to 7 ink system (depending on your printer) for black and white printing
only. I think the price includes software and inks but no printer, the
system only works with certain printers. It has gotten very favorable
reviews in fine art photo magazines (May/June 2005 issue of Photo
Techniques, the review might be on the magazine's website
(phototechmag.com) or on Lyson's). Lyson will send you some sample
prints if you register at their website. I suspect that if someone sent
Lyson a digital TEM or SEM image they might make a print of that as a
sales tool. I'll bet they have no idea that there is a market for their
products in the EM field. I only wish I did more than a few prints per
year of parts of ground up cells.
Lyson is not the only firm making inks, papers and printing systems
for fine art B&W photography. Check out InkjetMall.com or MediaStreet.com.
If you want to stick with an ink jet and the manufacturer's inks and
papers the Canon i9900 is the Editor's Choice in the June 28 2005 issue
of PC magazine. The same printer is also the first choice in Photo
Techniques May/June 2005 issue. Someone on the list also liked this
printer.
If you like HP printers the PhotoSmart 8450 will take an HP 'photo
gray' cartridge set for doing black and white prints.
Disclaimer: I don't have any financial interest in any of the firms
or products I mentioned.

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: scott.coutts-at-med.monash.edu.au
Date: Tue, 19 Jul 2005 09:53:59 -0500
Subject: [Microscopy] Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

hoffpajo-at-yahoo.com wrote:
}
} there is a very simple way to look at bacteria in the
} SEM. just puch teh bacteria/liquid through a .45
} micron filter. then process the filter paper for the
} SEM. this is a tried and true method.
} john
}

Yes, but that doesnt help me to lay mats of bacteria onto a support -
filtering them is what we normally do for the free-living cells, but if
I filter the mats, firstly there's a good chance that they;ll get stuck
in the syringe, and secondly, they won't come out flat on the filter.
THey'll be all bunched up or folded.

--
Scott J. Coutts
---------------------------------------------------------------
Bacterial Pathogenesis Research Group
Department of Microbiology,
Box 53, Monash University, 3800, Australia
Phone: 9905 4838 Fax: 9905 4811
---------------------------------------------------------------

==============================Original Headers==============================
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From: peoshel-at-wisc.edu
Date: Tue, 19 Jul 2005 10:19:15 -0500
Subject: [Microscopy] Re: Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roger,

This is interesting information. Would you be willing to write it up
for MIcroscopy Today? With illustrations and all. We are interested
in running such an article, and there would be room for the details
and so forth that you can't put in an email.

Phil
(also still not entirely retired from Tech Ed. at Microscopy Today)

} Thanks everyone, I've gotten lots of helpful advice and comments, so
} I'll try to sum up my conclusions.
}
} Some background: Silicon carbide (SiC) is produced by a very old
} company Washington Mills located near Niagara Falls but production is
} in Illinois. Blessed folks; they sent me a sample of crystals for free.
}
} {http://www.medibix.com/company.jsp?company_id=10001791}
}
} It is made in tonnage quantities as clusters of glossy flat hexagonal
} black crystals up to a few centimeters across, and which are actually
} deep transparent blue and are crushed for abrasives and metallurgy.
} The cost of this industrial crystalline material is negligible. The
} Cree Corporation in NC makes also clear white SiC crystals and wafers
} for a fairly high price, but only sells them already faceted into
} clear diamond substitutes for jewelry. Such clear SiC crystals are
} termed "moissenite".
}
} SiC crystals are very hard and brittle and the edge breaks when you
} try to sharpen them into knives using anything resembling normal gem
} faceting methods. But knives can be made using the proper lapping
} technique and the appropriate grades of diamond powder. Here is an
} interesting link on modern diamond powder technology:
}
} {http://www.ceramicindustry.com/CDA/ArticleInformation/features/
} BNP__Features__Item/0,2710,152388,00.html}
}
} At any rate, besides glass, only hard single crystals of diamond,
} sapphire, and silicon carbide seem to be appropriate for making
} ultramicrotome knives, which by their nature demand a perfectly
} homogeneous material. Sapphire knives were sold at one time by
} Diatome but the price was reputedly about $500 apiece and they became
} dull, unlike diamond knives, so the economics was questionable.
}
} My SiC knives become dull after a few hundred slices, probably
} depending on the the hardness of the embedding plastic (glycol
} methacrylate works well), but the raw materials are inexpensive and
} my lapping machine only cost about $50 to build, so the labor of
} making them is inherently the major cost.
}
} Since SiC crystals are difficult to sharpen without breaking the
} edge, I assume the exact same lapping techniques would also be
} appropriate for sapphire, which is softer tougher and less brittle
} than SiC; the latter are the very devil to sharpen without breaking.
} I learned how to make them through sheer stubbornness in order to
} make many serial sections with my Porter Blum MT-2 without paying
} $1000+ for a diamond knife.
}
} I can only guess at the durability of sapphire knives, but the fact
} that they were once offered indicates that they might still be a
} viable alternative for some applications if they were only available
} at a lower cost.
}
} My SiC crystals are epoxyed in a slot cut in the apex of a steel
} holder the same size and shape as a right angle glass knife. The
} clean crystals are hydrophilic (I assume the exposed surface layer at
} the atomic level is silicon oxide). I clean them by wiping the edge
} sideways with Teflon and don't have many wetting problems.
}
} My conclusion is that I don't much want to go the trouble of trying
} to make money patenting, licensing and selling knives, but that I
} should publish my technique of making them, most likely in the Review
} of Scientific Instruments, and see if the world pays attention. I
} don't think the method of sharpening hard crystals to give very sharp
} edges has ever been published; I can't find it in the scientific
} literature. I seriously doubt it would work for diamonds without
} modification.
}
} The only possible drawback to open publication is that no one maker
} could make a lot of money making them, and thus might not offer them,
} assuming they prove to be a modestly useful alternative to diamonds.
}
} I assume that maybe the Chinese could sell disposable knives for $20
} or so and they might catch on for student use and low budget and
} occasional use. Glass knives are clearly sharper for a few dozen
} sections and diamond knives clearly last longer.
}
} -- Roger Baker Jr.
} 1303 Bentwood,
} Austin Tx, 78722
}
}
}
}
}
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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} 29, 19 -- Subject: Re: [Microscopy] Alternatives to diamond knives?
} 29, 19 -- Date: Mon, 18 Jul 2005 21:17:44 -0500
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

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From: MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 19 Jul 2005 10:20:44 -0500
Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I second Sousan's reply. It saves downloading a lot of different emails! I
also found the information quite helpful.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Monday, July 18, 2005 5:22 PM
To: Sherwood, Margaret

Dear Listers,

Below is a compilation of the replies I received to my recent question
about Cu vs. Ni grids for immunolabeling. I usually don't do this
without express permission, but most everybody posted to the list
anyway, and I made sure to edit those that didn't. If it bothers
anyone, let me know and I will never do it again.

In summary, it appears that copper can and will react with salts in
buffers and other solutions, although some people still have decent
luck. This problem can be ameliorated by using coated Cu grids and only
wetting the coated side and by shortening incubation times

Also, Marc Pypaert had the interesting suggestion of just using Ni grids
for everything, since they're not much more expensive, thereby avoiding
the problem entirely. Any thoughts on this?

Many thanks to everyone who replied! I now have a much better response
to our clients' questions on this matter (not to mention my own).

Cheers,
Randy


Over the years I have frequently seen cases where the samples themselves
have reacted with the copper grid. Two examples:

1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
precipitates were ugly and analyzed as copper sulphide. Our surmise was
that the iron and copper had undergone ion exchange while the grid (and
carbon film) was wet with the culture medium. When we used nickel
grids, the precipitates were well formed and composed of iron and
sulphur.

2) Looking at Si precipitates in Al-Si electronic bond wire. The
specimens were prepared by embedding and microtoming, floating in DI
water. The Si precipitates were surrounded by an ugly mess containing
masses of copper. Again, presumed to me electrochemical reaction
between the Al and the Cu grid, through the DI water medium, somehow
preferentially at the Si precipitates. Again, use of Ni grids produced
good pictures allowing us to characterize the precipitation.

Having said all that, we continue to default to use copper grids!

Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA


-------------------------------------------

The main problem encountered with copper grids used for
immunocytochemistry or immunogold labeling is the reaction of the copper
with salts in the buffers used during labeling. This is a time-related
reaction, and can usually be avoided by having short labeling runs and
using grids with films on them. Gilder grids, available from major
microscope supply vendors, are gold-coated copper grids, and are not
that much more expensive than regular copper grids. These grids may be
the variety used by researchers who have reported having no problems
with their grids during their immuno runs.
I usually use nickel grids for my work, but have used
formvar-coated copper grids without problems for 1 day immuno runs so
long as the film is intact and as long as I don't get clumsy and end up
sinking my grids in my solutions (if I do sink them, I do a quick
distilled water rinse, blot the back of the grids with filter paper
until almost dry, then re-float them where I left off). As others have
shared, the nickel grids are much sturdier, not too expensive, and are
not a problem to handle as long as you use antimagnetic forceps. With
the nickel grids, remember to correct for astigmatism in the TEM by
using a hole in your sample on the nickel grid. This will accommodate
for any inherent residual magnetic field in the grid itself.

Edward Haller
Lab Manager, Diagnostic Electron Microscopy Lab University of South
Florida Pathology Department Tampa, FL 33612

------------------------------------------------------------

We have had problems with Cu grids, even Formvar coated ones. I have
precoated copper grids with Parlodion by dipping, blotting and drying
before use, with or with out a formvar coating. THat method seems to
protect the copper from reacting with PBS or whatever. This is not
original to me, but I cannot recall where I read this (among many other
things I can no longer recall). I think it might have been one of those
smart Swiss guys.

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program Scientific Director, Electron
Microscopy P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
-------------------------------------------------------------------

I had a bad experience with formvar-copper grids in immunogold
experiment many years ago when incubating primary antibody overnight and
the rest done on the following morning. I am using Nickel grids now --
no more problems.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
------------------------------------------------------------------------
------------

recently i did a long ultracentrifuge run to load some 10S particles
onto a formvar-copper grid. the grids reacted with the phosphate buffer
over the 4 hour spin time. not a pretty picture - i had to go back to
my collaborator and get fresh material so we could put it onto
formvar-nickel grids. wonderful gentleman.


Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
----------------------------------------------------------------

We used Cu grids for many years. Normally we use formvar + carbon
coated grids for ICC since we have a lot less loss of sections. We also
tend to do long incubations ...usually overnight. This is time
efficient and also lets us use highly diluted antibody so also minimize
background due to cross-reactions from contaminants in polyclonal
antibodies.

Occasionally we would have a reaction due to copper oxidizing with
resultant green solution. I attributed this to salts or traces of Tween
20 in the buffer and breaks in the coating exposing the Cu. As far as I
can see, this is the only reason for not using Cu grids if you use
coated grids. We have switched for the most part to using coated Ni
grids to avoid this problem.

On the other hand, occasionally we need to do a double labeling using
uncoated grids and both sides of the sections. I would hesitate to use
Cu for this and prefer Ni. Gold would work but is both more expensive
and more delicate than Ni grids.

Debby Sherman, Manager
Life Science Microscopy Facility
Purdue University
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
------------------------------------------------------------------------
-

X-from my experience, coated copper grids will be fine for most (if not
all) immunolabelling, providing the solutions do not wet the uncoated
side of the grid. Uncoated copper grids always have been fine when I've
used PBS.

However, copper grids (uncoated - or the uncoated side) usually will
react with Tris-HCl buffers and some of the high salt buffer
formulations that I've used. Longer incubation times (eg. overnight)
cause more reaction with the grid than shorter times (eg. 60mins) - so
you may be able to use copper grids without problems if incubations are
short, even with buffer formulations that might react with the grid if
left for longer periods. It also is more difficult to prevent wetting
of the reverse (uncoated) side of coated copper grids if incubation
times are long, and particularly if wetting agents are used in the
buffer formulation.

When I started immunolabelling in the early 1980s - and hadn't yet heard
about the use of nickel grids - I used uncoated copper grids (and PBS)
for years without problems. My current lab generally uses a high salt
Tris-HCl buffer which includes a small amount of Tween 20, so we
routinely use nickel grids for immunolabelling. Nickel grids do have
some disadvantages (eg. charge), but we run a multi-user facility, often
training students or other inexperienced users, we've found nickel the
best option to overcome potential "grid-reaction" problems. However,
nickel grids certainly aren't essential for successful immunolabelling -
you just need to be aware of the possible problems.

Dr Deborah Stenzel
Analytical Electron Microscopy Facility
Queensland University of Technology
GPO Box 2434
Brisbane 4001
Australia

------------------------------------------------

I know (from sad experience) that Tris buffers will etch Cu grids - the
antibody droplets turn a lovely blue and there is gunk all over the
sections. I use Ni grids, only because I prefer Tris buffers (no real
reason why, I guess) for IEM and my current PI cringes at buying Au
grids.
I know one person who does all of her labelling in phosphate buffer and
uses Cu grids with no precipitate problems.

As for the other reasons you Googled....I've heard them, but never seen
any documentation nor had personal experience.

Can't wait to hear if anyone has actual evidence for some of the other
no-Cu reasons!

Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
----------------------------------------------------------------

Hi Randy,
I had problems with Cu grids when incubating sections for long periods -
overnight for instance. The copper would react with whatever was
available and form green-blue salts. Not unexpected. We switched to
nickel grids for a while but they charge, gold but they are
delicate...by this time we had shortened the incubation times used, and
tried copper again... And had no problem using coated grids and
incubation times of an hour or so. Grids are floated on drops of media
so that only the coated side is wetted - I don't know if that is
important.

Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C


I only ran into the "use only Ni or Au grids" rule here, in my current
job, and my predecessor certainly produced some superb images showing
gold labelling of TEM sections. Blissfully unaware of this rule, I had
been using copper grids for all EM immunolabelling. To get good
labelling of one particular structure, which is about 40 nm diameter, I
used uncoated thin-bar Cu grids so I could get labelling on both sides
of the section - seemed to work just fine. I'll be interested to see
the responses to your question.

Dr. Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia








Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: baskin-at-bio.umass.edu
Date: Tue, 19 Jul 2005 10:32:56 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This sounds like a job for ESEM. How much magnification do you need?
You can work with fully hydrated samples. You can't get into the
ultrastructual range of mags but excellent imaging in the classic SEM
mag range, particularly with the newer line of ESEM's.

Tobias
}
}
} hoffpajo-at-yahoo.com wrote:
} }
} } there is a very simple way to look at bacteria in the
} } SEM. just puch teh bacteria/liquid through a .45
} } micron filter. then process the filter paper for the
} } SEM. this is a tried and true method.
} } john
} }
}
} Yes, but that doesnt help me to lay mats of bacteria onto a support -
} filtering them is what we normally do for the free-living cells, but if
} I filter the mats, firstly there's a good chance that they;ll get stuck
} in the syringe, and secondly, they won't come out flat on the filter.
} THey'll be all bunched up or folded.
}
} --
} Scott J. Coutts
} ---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
} ---------------------------------------------------------------
}
}


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

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From: acarol1-at-uic.edu
Date: Tue, 19 Jul 2005 10:33:51 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, How about using a wire loop to place a mat of cells on a poly-L-lysine
coated cover slip and then running it through fix, dehydration, etc....
Maybe you don't need the loop at all. Just slide the cover slip under a
mat and lift out of the solution. I've never done a mat of cells but I've
had success with SEM of cells from suspension on coated cover slips or
pieces of Fisher Brand Plus Slides.

Andy Carol
UIC Biology
Chicago, IL

On Tue, July 19, 2005 9:54 am, scott.coutts-at-med.monash.edu.au said:
}
}
}
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}
} hoffpajo-at-yahoo.com wrote:
} } there is a very simple way to look at bacteria in the
} } SEM. just puch teh bacteria/liquid through a .45
} } micron filter. then process the filter paper for the
} } SEM. this is a tried and true method.
} } john
}
} Yes, but that doesnt help me to lay mats of bacteria onto a support -
filtering them is what we normally do for the free-living cells, but if
I filter the mats, firstly there's a good chance that they;ll get stuck
in the syringe, and secondly, they won't come out flat on the filter.
THey'll be all bunched up or folded.
}
} --
} Scott J. Coutts
} ---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
} ---------------------------------------------------------------
}
} ==============================Original
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From: henriks-at-southbaytech.com
Date: Tue, 19 Jul 2005 10:38:55 -0500
Subject: [Microscopy] Re: MicroscopyListserverviaWWW: Looking T-Tool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Tong:

You can find them at: http://www.precisiontem.com/ttools.html

If you are looking for tools for precision cross sectioning, you may
also want to visit our website and look at the the following products:

Model 590 Tripod Polisher®
Model 595 BiPod Polisher™

The Model 590 Tripod Polisher® is based on the original design by IBM
East Fishkill and is used for SEM, TEM cross sectioning. The Model 595
BiPod™ Polisher is more similar to the T-tool although it incorporates
many of the unique and critical features of the original Tripod
Polisher® as well.

Please feel free to contact me off-line for details and pricing.

Best regards-

David

tantt-at-umich.edu wrote:

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From: gcc-at-couger.com
Date: Tue, 19 Jul 2005 13:43:33 -0500
Subject: [Microscopy] Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Unless you can get life from the knife that is comparable to
diamond or cost comparable to glass. The cost of labor make any
thing much more expensive than glass a very hard sell when a
diamond knife cost less than a week of a lab tech time not
including the work lost while hie was messing around with knives
when he could be doing more productive work.

Gordon Couger
Stillwater, OK
www.couger.com/gcouger

rcbaker-at-eden.infohwy.com wrote:
}
} On Jul 18, 2005, at 5:48 PM, sryazant-at-ucla.edu wrote:
}
} } Dear Roger
} } I am not sure is tungsten carbide is similar to SiC or not. My
} } experience
} } with tungsten carbide basically was negative - this knife starts
} } scratching
} } the surface after just 50+ 0.5 um sections of standard plastic
} } embedded
} } tissue (no hard inclusions etc). As manufacturer explained to me it's
} } because of polycrystalline nature of the material - small crystals
} } just
} } became loose and left the edge creating ruff surface. From this
} } point of
} } view, amorphous (like glass) or monocrystal (like diamond) material is
} } preferable for good sections. Sapphire (hardness is 9) knifes were
} } on the
} } market for while without great success. From economical point of
} } view,
} } tungsten carbide knifes were also not so good - $100 each with
} } ability to
} } produce only 50+ good sections. If SiC acts in the similar way,
} } then it
} } would be difficult to use it in EM applications. Another aspect of
} } using
} } exotic materials - is it hydrophilic or hydrophobic? Could you use
} } water
} } with them? How easy to clean it up and handle? Sergey
}
}
}
}
} Thanks everyone, I've gotten lots of helpful advice and comments, so
} I'll try to sum up my conclusions.
}
} Some background: Silicon carbide (SiC) is produced by a very old
} company Washington Mills located near Niagara Falls but production is
} in Illinois. Blessed folks; they sent me a sample of crystals for free.
}
} {http://www.medibix.com/company.jsp?company_id=10001791}
}
} It is made in tonnage quantities as clusters of glossy flat hexagonal
} black crystals up to a few centimeters across, and which are actually
} deep transparent blue and are crushed for abrasives and metallurgy.
} The cost of this industrial crystalline material is negligible. The
} Cree Corporation in NC makes also clear white SiC crystals and wafers
} for a fairly high price, but only sells them already faceted into
} clear diamond substitutes for jewelry. Such clear SiC crystals are
} termed "moissenite".
}
} SiC crystals are very hard and brittle and the edge breaks when you
} try to sharpen them into knives using anything resembling normal gem
} faceting methods. But knives can be made using the proper lapping
} technique and the appropriate grades of diamond powder. Here is an
} interesting link on modern diamond powder technology:
}
} {http://www.ceramicindustry.com/CDA/ArticleInformation/features/
} BNP__Features__Item/0,2710,152388,00.html}
}
} At any rate, besides glass, only hard single crystals of diamond,
} sapphire, and silicon carbide seem to be appropriate for making
} ultramicrotome knives, which by their nature demand a perfectly
} homogeneous material. Sapphire knives were sold at one time by
} Diatome but the price was reputedly about $500 apiece and they became
} dull, unlike diamond knives, so the economics was questionable.
}
} My SiC knives become dull after a few hundred slices, probably
} depending on the the hardness of the embedding plastic (glycol
} methacrylate works well), but the raw materials are inexpensive and
} my lapping machine only cost about $50 to build, so the labor of
} making them is inherently the major cost.
}
} Since SiC crystals are difficult to sharpen without breaking the
} edge, I assume the exact same lapping techniques would also be
} appropriate for sapphire, which is softer tougher and less brittle
} than SiC; the latter are the very devil to sharpen without breaking.
} I learned how to make them through sheer stubbornness in order to
} make many serial sections with my Porter Blum MT-2 without paying
} $1000+ for a diamond knife.
}
} I can only guess at the durability of sapphire knives, but the fact
} that they were once offered indicates that they might still be a
} viable alternative for some applications if they were only available
} at a lower cost.
}
} My SiC crystals are epoxyed in a slot cut in the apex of a steel
} holder the same size and shape as a right angle glass knife. The
} clean crystals are hydrophilic (I assume the exposed surface layer at
} the atomic level is silicon oxide). I clean them by wiping the edge
} sideways with Teflon and don't have many wetting problems.
}
} My conclusion is that I don't much want to go the trouble of trying
} to make money patenting, licensing and selling knives, but that I
} should publish my technique of making them, most likely in the Review
} of Scientific Instruments, and see if the world pays attention. I
} don't think the method of sharpening hard crystals to give very sharp
} edges has ever been published; I can't find it in the scientific
} literature. I seriously doubt it would work for diamonds without
} modification.
}
} The only possible drawback to open publication is that no one maker
} could make a lot of money making them, and thus might not offer them,
} assuming they prove to be a modestly useful alternative to diamonds.
}
} I assume that maybe the Chinese could sell disposable knives for $20
} or so and they might catch on for student use and low budget and
} occasional use. Glass knives are clearly sharper for a few dozen
} sections and diamond knives clearly last longer.
}
} -- Roger Baker Jr.
} 1303 Bentwood,
} Austin Tx, 78722
}
}
}
}
}
}
}
}
}
}
}
}
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From: tonygr-at-MIT.EDU
Date: Tue, 19 Jul 2005 14:03:32 -0500
Subject: [Microscopy] Position for microscopist at MIT (for your review)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Center for Materials Science and Engineering at the Massachusetts
Institute of Technology has an immediate vacancy for a research specialist
in electron microscopy. A description of the vacancy, together with MIT's
employment policies, benefits, and other information, with links for
on-line application is available at the following URL:
http://sh.webhire.com/servlet/av/jd?ai=631&ji=1620589&sn=I
or applications may be forwarded by electronic or paper mail to Ms.
Jennifer Crockett, CMSE Assistant Director, Room # 13-2106, Massachusetts
Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307,
e-mail crockett-at-mit.edu.

The application review process will be open until a qualified candidate has
been identified. Questions about this position may be addressed to the
undersigned.

Tony Garratt-Reed



***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


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From: sryazant-at-ucla.edu
Date: Tue, 19 Jul 2005 16:01:12 -0500
Subject: [Microscopy] Re: dye sub, ink jet etc for prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I used to work with Lyson "grey" inks and paper. It was previous
generation, not "Daylight Darkroom" line. The quality of images was (yes,
was) outstanding - no comparison to laser or dye-sub. The problem appeared
to happens month later - the images faded very quickly: in month they lost
about 50% of tone. I was trying different type of paper - no luck. Right
now I am trying "grey" inks from different vendor, so I'll see. As far as
I understand from numerous conversation with people from graphic design and
digital photography area, it's practically impossible to protect ink-jet
images from fading if stored not in album with protective cover. Another
thing: glossy paper always bad in terms of fading. Matte paper is the
best. Pigment inks are better than organic, but tends to clog the printer's
head. After HP patent on ink-jet technology expired, Epson jumped into the
market with few serious enhancements of the HP technology. I think they
used piezo-element to disperse the inks and probably some other tricks. I
am very happy with Epson 890 model. It was quite expensive a few years
ago. You could get it for 10$ at eBay now. It has 6 inks and quality of
color (with Epson cartridges) and B&W prints just outstanding. It's quite
slow and as I mentioned above B&W images with Lyson inks tends to fade
quick. No interest in Epson, just happy user. Sergey

At 07:34 AM 7/19/2005, you wrote:



} ----------------------------------------------------------------------------
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From: K.venner-at-ion.ucl.ac.uk
Date: Tue, 19 Jul 2005 16:45:01 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: sem prep of floating cell mats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Tuesday, July 19, 2005 at 10:11:07
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: kerrie

Organization: ION, UCL UK

Title-Subject: [Filtered] sem prep of floating cell mats

Question: Never done this for sample prep, but it's a mind experiment: how about putting the cells and their media into a vessel like a bath with a plug at the bottom. Submerge the millipores and when they are sitting on the bottom, let the media out via the plug hole. I would say that the cell mats would settle gently with few creases. Sandwich lightly in a cut-down Swinnex holder, process and cpd intact. Just an idea from the old way I learned to coat grids with formvar.

---------------------------------------------------------------------------

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From: peoshel-at-wisc.edu
Date: Wed, 20 Jul 2005 08:20:17 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Phil,

Would not critical point drying them in one of our microporous specimen
capsules be the best approach? See URL
http://www.2spi.com/catalog/instruments/microporous.shtml

Chuck
----- Original Message -----
X-from: {peoshel-at-wisc.edu}
To: {cgarber-at-2spi.com}
Sent: Tuesday, July 19, 2005 10:13 AM

Chuck,

Well, he wants to try HMDS, and that works very well for bacteria.
CPD is extra handling, and he's dealing with fragile biofilm mats, so
that's another reason. It can work, but the less handling the better.
I also don't use the porous capsules anymore. Too much trouble with
staticy specimens after drying, and too many staticy little white
bits of capsule coming off and getting on the specimens.

Phil

} Hello Phil,
}
} Would not critical point drying them in one of our microporous specimen
} capsules be the best approach? See URL
} http://www.2spi.com/catalog/instruments/microporous.shtml
}
} Chuck

} } Scott,
} }
} } Interesting problem. The "matts" you refer to are likely to fragment
} } or worse if you use the usual filter-based preparation methods .
} } First question: do you have access to cryoSEM? If yes, this would be
} } the best way to do your samples. Freeze in a high-pressure freezer,
} } if available, or by plunging into slush nitrogen, then do the cryo
} } SEM. This is the method most likely to give you the unaltered
} } structure of the matts and the critters therein.
} } If you don't have access to cryoSEM, then the best way is to use
} } minifuge tubes or the like. Let the samples sit in the tube in fix,
} } then the EtOH dehydration steps, being very careful when you withdraw
} } the fluid to change it. Add the fluid for the next step as you
} } withdraw the old fluid to minimize disturbance of the matts, and
} } maybe add the fluid for each succeeding step down the side of the
} } tube.
} } I suggest a 2:1 1:1 1:2 EtOH:HMDS series before the 3 changes in 100%
} } HMDS.
} } I've found it helpful to deposit the last HMDS + sample drops on a
} } sputter-coated membrane filter* stuck to a SEM stub for drying. This
} } further minimizes handling.
} } * 0.22 micron "nucleopore" type -- the ones with the nice, round
} } holes, not the torturous-path type of filter. Sputter coat both sides
} } of the filter before sticking to the stub, best, or stick to the stub
} } with conductive carbon tabs, then sputter coat. Then drop on the
} } samples in HMDS. (And yes, sputter coat for viewing in the SEM.)
} }
} } Phil
} }
} } } Hi All,
} } }
} } } I need to prep some samples for SEM, but I'm not sure of the best way to
} } } go about it.
} } }
} } } We have some bacteria that normally grow in a type of liquid nutrient
} } } medium as free, discrete and seperate motile cells. However, we have
} } } come across a strain that forms into 'mats' on the bottom of the culture
} } } vessel. It is these that we would like to examine by SEM.
} } }
} } } The problem is that the 'mats' are not like a normal biofilm in that
} } } they are not strongly adherant to a solid surface, and the mat is not
} } } very robust. The mats will 'float' off the bottom of the culture vessel
} } } if they are disturbed and they will fragment into small flakes if the
} } } vessel is shaken or swirled. The fragments are usually a milimetre or so
} } } square.
} } }
} } } Of course, the 'flakes' need to be flat in order to view them properly.
} } } I thought I might be able to pipette them, in a drop of the media
} } } they're already in, onto nucleopore membranes and allow them to settle,
} } } then try to remove the rest of the media and hope that they stick
} } } through the dehydration process... but i'm not sure they will. I'm
} } } planning on using HMDS.
} } }
} } } I'm goig to try it just to see what happens... but has anyone prepared a
} } } sample like this that could suggest something that is know to work well?
} } }
} } } Cheers,
} } }
} } } --
} } } Scott J. Coutts
} } } ---------------------------------------------------------------
} } } Bacterial Pathogenesis Research Group
} } } Department of Microbiology,
} } } Box 53, Monash University, 3800, Australia
} } } Phone: 9905 4838 Fax: 9905 4811
} } } ---------------------------------------------------------------
} } }
} } } ==============================Original
} } } Headers==============================
} } } 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005
} } } 8, 17 -- Received: from omta04sl.mx.bigpond.com
} } } (omta04sl.mx.bigpond.com [144.140.93.156])
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} } } 01:46:02 -0500
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} } } 06:46:00 +0000
} } } 8, 17 -- Message-ID: {42DCA1AB.5070904-at-med.monash.edu.au}
} } } 8, 17 -- Date: Tue, 19 Jul 2005 16:46:03 +1000
} } } 8, 17 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au}
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} } } ==============================End of -
} } } Headers==============================
} }
} } --
} } Philip Oshel
} } Supervisor, BBPIC microscopy facility
} } Department of Animal Sciences
} } University of Wisconsin
} } 1675 Observatory Drive
} } Madison, WI 53706
} } voice: (608) 263-4162
} } fax: (608) 262-5157 (dept. fax)
} } http://www.ansci.wisc.edu/microscopy.htm
} }
} } ==============================Original
} } Headers==============================
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==============================Original Headers==============================
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From: RossLM-at-missouri.edu
Date: Wed, 20 Jul 2005 08:35:36 -0500
Subject: [Microscopy] Re: HR images with Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ezer,

Do you want to use an imaging program or by x-ray microanalysis? If
imaging, one shareware program you can use is Image J from NIH. Do a
search for image j and it will take you to the download site. For
simple measurements all you need to do is select the straight line
tool, draw a line over the scale bar, and then set scale from a pull
down menu. After that, with the same tool to draw a line over the
distance you want to measure, keeping the mouse button down, and in
the information field will be the line distance.

If by microanalysis, there is a program called GMR Film in the MSA
library that you can download. It is based on K-ratio measurements so
you need standards, and the film has to be smaller than the
interaction volume. It is an old DOS program but works with XP, and
I'm sure there are newer programs pout there too.

If you need any other help, feel free to contact me off-line,
Lou Ross


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=2227
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu

==============================Original Headers==============================
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From: westlab-at-linkline.com
Date: Wed, 20 Jul 2005 17:49:14 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: Tegal Plasmod etcher manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (westlab-at-linkline.com) from http://www.microscopy.com/MLFormMail.html on Wednesday, July 20, 2005 at 16:12:07
---------------------------------------------------------------------------

Email: westlab-at-linkline.com
Name: Mike Maladzhikyan

Organization: Western Analytical Lab

Title-Subject: [Filtered] Tegal Plasmod etcher manual

Question: Hi,

I am wondering if anyone has the Tegal Plasmod manual available? We acquired this etcher on the used market and it did not come with a manual. Please email me. I will be happy to pay you for your time and the copying costs. Thanks.

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Wed Jul 20 17:49:14 2005
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7, 12 -- Subject: MicroscopyListserverviaWWW: Tegal Plasmod etcher manual
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: Joseph_Oparowski-at-bose.com
Date: Thu, 21 Jul 2005 05:56:38 -0500
Subject: [Microscopy] Recall: MicroscopyListserverviaWWW: Tegal Plasmod etcher manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The sender would like to recall the message, "[Microscopy] MicroscopyListserverviaWWW: Tegal Plasmod etcher manual".


==============================Original Headers==============================
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From: Diane.Ciaburri-at-gd-ais.com
Date: Thu, 21 Jul 2005 10:36:39 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: Tegal Plasmod etcher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've got one. It's only 8 pages long so it won't take long to scan it
in. I'll send it shortly.

Diane Ciaburri

-----Original Message-----
X-from: westlab-at-linkline.com [mailto:westlab-at-linkline.com]
Sent: Wednesday, July 20, 2005 6:49 PM
To: Ciaburri, Diane A

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (westlab-at-linkline.com) from
http://www.microscopy.com/MLFormMail.html on Wednesday, July 20, 2005 at
16:12:07
------------------------------------------------------------------------
---

Email: westlab-at-linkline.com
Name: Mike Maladzhikyan

Organization: Western Analytical Lab

Title-Subject: [Filtered] Tegal Plasmod etcher manual

Question: Hi,

I am wondering if anyone has the Tegal Plasmod manual available? We
acquired this etcher on the used market and it did not come with a
manual. Please email me. I will be happy to pay you for your time and
the copying costs. Thanks.

------------------------------------------------------------------------
---

==============================Original
Headers==============================
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==============================Original Headers==============================
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From: lcgould-at-med.cornell.edu
Date: Thu, 21 Jul 2005 13:46:10 -0500
Subject: [Microscopy] Re: gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,
Here's the problem I hope someone out there can help me with....
I have a set of samples that I need to embed for TEM. I've tried
previous sets, with poor results. The samples are cells plated on
collagen gels or on fibronectin in those 35mm cover-slip bottomed
dishes (available commercially). The cells have been pfa fixed,
antibody labelled with peroidase-DAB as the chromogen, then fixed in
4% buffered glutaradehyde. The problem I've had is that in the end,
the blocks have come out gummy rather than hard, so that they cannot
be sectioned.
I took some plain dishes and tested them out with both the Spurr's
and LX-112. The blocks were fine, so the problem is not an
interaction between the plastic of the dish and the resin (I embed by
over-filling the BEEM capsule and inverting the sample over the
meniscus of the resin).
I have extended the dehydration steps to 2 changes of 15 minutes each
at each of the ethanol concentrations, used a new bottle of 100% at
the end, and left the samples to infiltrate with the resin before
placing them in the oven.
This week I tried 2 sample dishes. One seems like regions of the
block face will cut well, but there may not be enough material to cut
away a piece to re-embed so that I can cut both en face and cross
sections. The second dish is gummy...the dish pulled off leaving a
rough surface (vs snapping off and leaving a clean , smooth face).
I can't use PO at the end of the dehydration because it will dissolve
the dish, same for acetone as a dehydrant. Aside from moving to
Phoenix (for both lower humidity and anonymity), I am at a loss.
Any ideas?
Thanks,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
1, 22 -- Subject: Re: gummy blocks
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From: hbarwood-at-troy.edu
Date: Thu, 21 Jul 2005 14:02:29 -0500
Subject: [Microscopy] Mechanical repair help?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have an old Simplex microscope (actually Leitz,) with one of the massive
stands used for this type of measuring microscope. The micrometer height
adjustment went out on me years ago and I've just been using the threaded
height adjustor on the column to focus the scope. Now, I'm at a point where
I really need the fine adjustment. Is there anyone out there who can
disassemble the focusing rack that holds the scope and replace the small
ball bearings so it will function again? If anyone can help, please let me
know. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu


==============================Original Headers==============================
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From: Winston.Wiggins-at-cshs.org
Date: Thu, 21 Jul 2005 14:33:59 -0500
Subject: [Microscopy] Re: gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Leona,
What time and temperature did you use to cure the blocks? You can try using
a lower temperature for a longer time to polymerize the blocks. That's
worked for me when I've had gummy bears ~, ah, blocks, that is!
Winston

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, E.M. Pathology Asst.
CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
Electron Microscopy, LLSPT, A823-A828
8700 Beverly Blvd.
Los Angeles, CA 90048
310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
Winston.Wiggins-at-CSHS.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


-----Original Message-----
X-from: lcgould-at-med.cornell.edu [mailto:lcgould-at-med.cornell.edu]
Sent: Thursday, July 21, 2005 11:51 AM
To: Winston.Wiggins-at-CSHS.org

Hi All,
Here's the problem I hope someone out there can help me with....
I have a set of samples that I need to embed for TEM. I've tried
previous sets, with poor results. The samples are cells plated on
collagen gels or on fibronectin in those 35mm cover-slip bottomed
dishes (available commercially). The cells have been pfa fixed,
antibody labelled with peroidase-DAB as the chromogen, then fixed in
4% buffered glutaradehyde. The problem I've had is that in the end,
the blocks have come out gummy rather than hard, so that they cannot
be sectioned.
I took some plain dishes and tested them out with both the Spurr's
and LX-112. The blocks were fine, so the problem is not an
interaction between the plastic of the dish and the resin (I embed by
over-filling the BEEM capsule and inverting the sample over the
meniscus of the resin).
I have extended the dehydration steps to 2 changes of 15 minutes each
at each of the ethanol concentrations, used a new bottle of 100% at
the end, and left the samples to infiltrate with the resin before
placing them in the oven.
This week I tried 2 sample dishes. One seems like regions of the
block face will cut well, but there may not be enough material to cut
away a piece to re-embed so that I can cut both en face and cross
sections. The second dish is gummy...the dish pulled off leaving a
rough surface (vs snapping off and leaving a clean , smooth face).
I can't use PO at the end of the dehydration because it will dissolve
the dish, same for acetone as a dehydrant. Aside from moving to
Phoenix (for both lower humidity and anonymity), I am at a loss.
Any ideas?
Thanks,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: Wade.McFaddin-at-NextekInc.com
Date: Thu, 21 Jul 2005 14:40:11 -0500
Subject: [Microscopy] Mechanical repair help?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Henry,

We have used Vashaw Scientific in Norcross, Ga. for service and repair on
our Leica/Leitz microscopes. The service engineer's name is David Benjamin,
phone number is 770-447-5632 and email address is "dbenjamin-at-vashaw.com"

I have no vested interest in Vashaw Scientific, but have enjoyed excellent
sales support and service from them for over 20 years.

Good luck,
Wade McFaddin
Nextek Inc.
Madison, Al.

-----Original Message-----
X-from: hbarwood-at-troy.edu [mailto:hbarwood-at-troy.edu]
Sent: Thursday, July 21, 2005 2:03 PM
To: wade.mcfaddin-at-nextekinc.com

I have an old Simplex microscope (actually Leitz,) with one of the massive
stands used for this type of measuring microscope. The micrometer height
adjustment went out on me years ago and I've just been using the threaded
height adjustor on the column to focus the scope. Now, I'm at a point where
I really need the fine adjustment. Is there anyone out there who can
disassemble the focusing rack that holds the scope and replace the small
ball bearings so it will function again? If anyone can help, please let me
know. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu


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12, 11 -- From Wade.McFaddin-at-NextekInc.com Thu Jul 21 14:40:11 2005
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From: mcauliff-at-umdnj.edu
Date: Thu, 21 Jul 2005 14:44:06 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Lee:

I have found that at least one Spurr component (DER or ERL, maybe
both) does react with plastic culture dishes over a few hours so that
may be the cause. I tested the components individually, not the final
mixture. I don't know about LX112. You can skip intermediate slovents
like PO with Epon substitutes, Epon is miscible with ethanols even with
some water remaining. When I did cultures I went from abs. EtOH to an
EtOH:Epon mix (2:1 first, then 1:2 each for an hour) with agitation
(slowly on a shaker table or just tilting the dish by hand every so
often), then several changes of pure Epon several hours each, at least
one under vacuum.
You should also check your accelerator, replace if more than 6
months old. Also, I don't use DMP-30, I use BDMA instead. I don't see
anything in your protocol that should cause problems. What happens if
you allow the epoxy to polymerize by itself, no contact with culture dish?

Geoff

lcgould-at-med.cornell.edu wrote:

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From: thoward-at-unm.edu
Date: Thu, 21 Jul 2005 14:50:25 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've had this problem with cultures grown on Matrigel- as well as plain
old collagen-coated plates. These substrates are very hydrated, so
extending your dehydration times and doing a few extra changes of
absolute, dry ethanol (or acetone) at the end should take care of the
gummy-block syndrome.

Good luck!

Tamara

On Thu, 21 Jul 2005 lcgould-at-med.cornell.edu wrote:

}
}
}
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}
} Hi All,
} Here's the problem I hope someone out there can help me with....
} I have a set of samples that I need to embed for TEM. I've tried
} previous sets, with poor results. The samples are cells plated on
} collagen gels or on fibronectin in those 35mm cover-slip bottomed
} dishes (available commercially). The cells have been pfa fixed,
} antibody labelled with peroidase-DAB as the chromogen, then fixed in
} 4% buffered glutaradehyde. The problem I've had is that in the end,
} the blocks have come out gummy rather than hard, so that they cannot
} be sectioned.
} I took some plain dishes and tested them out with both the Spurr's
} and LX-112. The blocks were fine, so the problem is not an
} interaction between the plastic of the dish and the resin (I embed by
} over-filling the BEEM capsule and inverting the sample over the
} meniscus of the resin).
} I have extended the dehydration steps to 2 changes of 15 minutes each
} at each of the ethanol concentrations, used a new bottle of 100% at
} the end, and left the samples to infiltrate with the resin before
} placing them in the oven.
} This week I tried 2 sample dishes. One seems like regions of the
} block face will cut well, but there may not be enough material to cut
} away a piece to re-embed so that I can cut both en face and cross
} sections. The second dish is gummy...the dish pulled off leaving a
} rough surface (vs snapping off and leaving a clean , smooth face).
} I can't use PO at the end of the dehydration because it will dissolve
} the dish, same for acetone as a dehydrant. Aside from moving to
} Phoenix (for both lower humidity and anonymity), I am at a loss.
} Any ideas?
} Thanks,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
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} 1, 22 -- Date: Thu, 21 Jul 2005 14:46:00 -0400
} 1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
} 1, 22 -- Subject: Re: gummy blocks
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} ==============================End of - Headers==============================
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|

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From: redhair-at-stanford.edu
Date: Thu, 21 Jul 2005 16:03:40 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Lee .Can you remove the coverslip from the dish after fixation and
process it alone? That way you could embed the whole coverslip in a Chang
mold, remove the glass using HF and punch out cells from the wafer. You
could also re-embed chips to cross-section. You would avoid the dish problem.
I have a feeling that your problems could be due to solutions getting
trapped under the coverslip (I am not familiar with this type of dish) or
sometimes the coating on the coverslip causes problems with removing the
inverted Beem capsules. The whole coating comes off leaving the rough
surface you mention. You might also want to check your resin and make sure
it is freshly made. Good luck, JoAnn
01:49 PM 7/21/2005 -0500, you wrote:



} ----------------------------------------------------------------------------
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Department of Molecular and Cellular Physiology
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Stanford, CA 94305
650-723-5856


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From: mpease-at-jhmi.edu
Date: Thu, 21 Jul 2005 16:08:21 -0500
Subject: [Microscopy] Re: gummy blocks (Out of the office/lab)

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the lab from Friday, July 22 through Friday, August 5, 2005.

If you need to speak with someone regarding the Microscopy and Imaging Core Facility, please contact our microscopy specialist, Rhonda Grebe, at 5-2597 for assistance; otherwise I will get back to you on Monday, August 8.

Mary Ellen


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From: psconnel-at-sas.upenn.edu
Date: Thu, 21 Jul 2005 16:46:32 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
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Lee,
Those small well dishes do not do so well when trying to exchange fluids. I
have a few suggestions and you may wish to try one or all.

1. I use a rotating table so that the fluids are swirling during the time that
they are sitting.
2. The dehydration times for single cells seems fine but adding the gel with
cells on top is equivalent to thin blocks so that I would suggest doubling the
time in 100% EtOH.
3. For many years I have been using HPMA (Hydroxypropyl Methacrylate)in place
of Propylene Oxide both after the 100% EtOH and in a 1:1 and a 2:1::Epon:HPMA
mixture before going into 100% Epon at least 3 times before embedding.

I do not know the reference for using HPMA. I was instructed to use it in
plastic Tissue Culture dishes when I first learned embedding back in 1971.
(Thanks to whomever the first one was who tried it!)

I agree that the LX-112 works great as the substitute for the original epon
which does not melt the plastics that the dishes are made from.

Pat Connelly
Univ. of Pennsylvania
psconnel-at-sas.upenn.edu

} ----------------------------------------------------------------------------

} Here's the problem I hope someone out there can help me with....
} I have a set of samples that I need to embed for TEM. I've tried
} previous sets, with poor results. The samples are cells plated on
} collagen gels or on fibronectin in those 35mm cover-slip bottomed
} dishes (available commercially). The cells have been pfa fixed,
} antibody labelled with peroidase-DAB as the chromogen, then fixed in
} 4% buffered glutaradehyde. The problem I've had is that in the end,
} the blocks have come out gummy rather than hard, so that they cannot
} be sectioned.
} I took some plain dishes and tested them out with both the Spurr's
} and LX-112. The blocks were fine, so the problem is not an
} interaction between the plastic of the dish and the resin (I embed by
} over-filling the BEEM capsule and inverting the sample over the
} meniscus of the resin).
} I have extended the dehydration steps to 2 changes of 15 minutes each
} at each of the ethanol concentrations, used a new bottle of 100% at
} the end, and left the samples to infiltrate with the resin before
} placing them in the oven.
} This week I tried 2 sample dishes. One seems like regions of the
} block face will cut well, but there may not be enough material to cut
} away a piece to re-embed so that I can cut both en face and cross
} sections. The second dish is gummy...the dish pulled off leaving a
} rough surface (vs snapping off and leaving a clean , smooth face).
} I can't use PO at the end of the dehydration because it will dissolve
} the dish, same for acetone as a dehydrant. Aside from moving to
} Phoenix (for both lower humidity and anonymity), I am at a loss.
} Thanks,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College of Cornell University

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From: r.sims-at-auckland.ac.nz
Date: Thu, 21 Jul 2005 17:10:42 -0500
Subject: [Microscopy] Sticky Stage on 840A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

The stage on my JSM 840A is pushed by linkage from the micrometer
knob in one X direction, and pulled back by a spring in the other X
direction.

It has become a bit sticky, so that the spring pulls it back only slowly. Some
users find this disconcerting.

Should I clean it or lubricate it?

If the latter, with what?

Diff pump oil?


cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


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From: jehrman-at-mta.ca
Date: Thu, 21 Jul 2005 19:17:21 -0500
Subject: [Microscopy] Re: Sticky Stage on 840A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ritchie,
I find that if you take off the micrometer or undo the gears inside and take
the shaft right out of the feedthrough, you can clean the stiff black stuff
off the shaft with lab alcohol. Take out the o-ring and clean it and its
groove and then lubricate the o-ring lightly with Apiazon L high vacuum
grease. Check that the spring isn't gummed up, as well. If you cannot get
the shaft out, run it to one end and clean all that is exposed and then run
it to the other end and clean the rest. If you cannot get out the o-ring,
you can put a bit of high vacuum grease on the clean shaft and then run it
into the o-ring hole.
----- Original Message -----
X-from: {r.sims-at-auckland.ac.nz}
To: {mager-at-interchange.ubc.ca}
Sent: Thursday, July 21, 2005 3:14 PM

Hi Ritchie,

Had the same problem a while back on our 5600. Our serviceman told me to
use a bit of RP oil. I
took the micrometer completely off and ran it through it's entire
travel, adding a small amount (just a
few drops total) as I went. It didn't really help at first, but then it
must have coated the sticky part, because
it became silky smooth again. It appears, though, that once they start
doing this, they need to have this
done every year or so. Mine is starting to get a little stiff again.

Hope this helps,

Jim

r.sims-at-auckland.ac.nz wrote:

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--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



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From: garyeaston-at-scannerscorp.com
Date: Thu, 21 Jul 2005 19:47:04 -0500
Subject: [Microscopy] Re: Sticky Stage on 840A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ritchie,
Your problem is more than likely caused by a sticky universal "slip
joint" that connects the external X micrometer with the X stage slide,
not a sticky X bearing slide. You can check this by moving the
universal itself in a back & forth motion. I would guess that the "Y"
universal moves freely and the X universal is sticky. Or, I have seen
those anti-backlash springs get weak after 20 years or so. And ,
lubricating your stage with anything, even dry graphite is not a good
thing to do with regards to your vacuum system (cleanliness, ultimate
vacuum, sample contamination, etc).

Gary M. Easton
Scanners Corporation
3rd Party SEM Service

r.sims-at-auckland.ac.nz wrote:

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From: qxing-at-uno.edu
Date: Thu, 21 Jul 2005 22:02:30 -0500
Subject: [Microscopy] AskAMicroscopist: How to index high order Kikuchi lines of

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(qxing-at-uno.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 21, 2005 at 12:25:38
---------------------------------------------------------------------------

Email: qxing-at-uno.edu
Name: Qingfeng Xing

Organization: University of New Orleans

Education: Graduate College

Location: New Orleans LA 70148

Question: How to index high order Kikuchi lines of silicon?

Clear high order Kikuchi lines can be obtained by CBED at an orientation far from low-index zones. I found that it's difficult to index them even knowing the position of the direct beam on the Kickuchi map, as there are so many lines. Is there any procedure to do that?

Thank you very much.

---------------------------------------------------------------------------

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9, 13 -- From: qxing-at-uno.edu (by way of Ask-A-Microscopist)
9, 13 -- Subject: AskAMicroscopist: How to index high order Kikuchi lines of
9, 13 -- silicon?
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From: uti-at-direcpc.com
Date: Fri, 22 Jul 2005 06:21:11 -0500
Subject: [Microscopy] Nikon D-70 for optical microscopy

Contents Retrieved from Microscopy Listserver Archives
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Dear Group,

We plan to make a modification for our optical motorized
microscope and consider an option to go with Nikon D-70.
If any one could share experience to adopt such camera
for science, would you please share with me?
There is any software, which could control the camera?
Any preferences? Any other sources of information?
There is another cameras/software suitable for such upgrade?

Thank you very much,
Vlad



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From: emlabservices-at-cox.net
Date: Fri, 22 Jul 2005 06:29:20 -0500
Subject: [Microscopy] SEM: 840A Sticky Stage

Contents Retrieved from Microscopy Listserver Archives
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Listers,

To add oil to your stage is nothing more than accelerated back-streaming. If
the stage is sticking or "jumpy" when translating the sample, the best
approach is to minimally disassemble, thoroughly clean, re-surface ball
bearing races where necessary, inspect for worn parts, and reassemble as it
was.

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St.
Topeka, Kansas 66617-1780
785.246.1232 voice
785.246.0168 fax
www.emlabservices.com



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From: j.bilde-at-risoe.dk
Date: Fri, 22 Jul 2005 06:49:40 -0500
Subject: [Microscopy] AskAMicroscopist: How to index high order Kikuchi lines of

Contents Retrieved from Microscopy Listserver Archives
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Dear Qingfeng,

You can get various programs to simulate the HOLZ lines. You can also use the on-line resources developed by Stadelmann at Lausanne. The webaddress is:

http://cimesg1.epfl.ch/CIOL/ems.html

Best regards,
Jorgen.
{:::} --- {:::} --- {:::} --- {:::} --- {:::} --- {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



-----Original Message-----
X-from: qxing-at-uno.edu [mailto:qxing-at-uno.edu]
Sent: 22. juli 2005 05:04
To: j.bilde-at-risoe.dk

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(qxing-at-uno.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 21, 2005 at 12:25:38
---------------------------------------------------------------------------

Email: qxing-at-uno.edu
Name: Qingfeng Xing

Organization: University of New Orleans

Education: Graduate College

Location: New Orleans LA 70148

Question: How to index high order Kikuchi lines of silicon?

Clear high order Kikuchi lines can be obtained by CBED at an orientation far from low-index zones. I found that it's difficult to index them even knowing the position of the direct beam on the Kickuchi map, as there are so many lines. Is there any procedure to do that?

Thank you very much.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: clei-at-uiuc.edu
Date: Fri, 22 Jul 2005 08:41:49 -0500
Subject: [Microscopy] Re: AskAMicroscopist: How to

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ask Prof. Zhou Weilie at UNO. He knows the answer.


---- Original message ----
} Date: Thu, 21 Jul 2005 22:04:03 -0500
} From: qxing-at-uno.edu
} Subject: [Microscopy] AskAMicroscopist: How to index high
order Kikuchi lines of
} To: clei-at-uiuc.edu
}
}
}
}
} ----------------------------------------------------------------------------
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From: hoffpajo-at-yahoo.com
Date: Fri, 22 Jul 2005 11:51:03 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
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i haven't had the time to follow the thread so if
someone has suggested this then just ignore. you can
buy polyproplene dishes that work just as well so you
can use PO, there are also polypropylene cover slips
as well as slide, i thing the use of PO will solve
your gummy blocks
john

--- lcgould-at-med.cornell.edu wrote:

}
}
}
}
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}
} Hi All,
} Here's the problem I hope someone out there can
} help me with....
} I have a set of samples that I need to embed for
} TEM. I've tried
} previous sets, with poor results. The samples are
} cells plated on
} collagen gels or on fibronectin in those 35mm
} cover-slip bottomed
} dishes (available commercially). The cells have been
} pfa fixed,
} antibody labelled with peroidase-DAB as the
} chromogen, then fixed in
} 4% buffered glutaradehyde. The problem I've had is
} that in the end,
} the blocks have come out gummy rather than hard, so
} that they cannot
} be sectioned.
} I took some plain dishes and tested them out with
} both the Spurr's
} and LX-112. The blocks were fine, so the problem is
} not an
} interaction between the plastic of the dish and the
} resin (I embed by
} over-filling the BEEM capsule and inverting the
} sample over the
} meniscus of the resin).
} I have extended the dehydration steps to 2 changes
} of 15 minutes each
} at each of the ethanol concentrations, used a new
} bottle of 100% at
} the end, and left the samples to infiltrate with the
} resin before
} placing them in the oven.
} This week I tried 2 sample dishes. One seems like
} regions of the
} block face will cut well, but there may not be
} enough material to cut
} away a piece to re-embed so that I can cut both en
} face and cross
} sections. The second dish is gummy...the dish pulled
} off leaving a
} rough surface (vs snapping off and leaving a clean ,
} smooth face).
} I can't use PO at the end of the dehydration because
} it will dissolve
} the dish, same for acetone as a dehydrant. Aside
} from moving to
} Phoenix (for both lower humidity and anonymity), I
} am at a loss.
} Any ideas?
} Thanks,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core
} Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
} ==============================Original
} Headers==============================
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} 13:46:10 2005
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} {lcgould-at-med.cornell.edu}
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__________________________________________________
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From: walck-at-southbaytech.com
Date: Fri, 22 Jul 2005 12:07:28 -0500
Subject: [Microscopy] AskAMicroscopist: How to index high order Kikuchi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I only saw one response to your question, so I thought that I would jump
in and try to help. As was pointed out in the posting from Jorgen
Bilde, you can get a program to help you, but you can also do it
manually with some effort.

If you know the orientation of the beam, then you can figure out the
range of indices that match or closely matches the g "dot" =1 or 2
condition (where B is the beam direction). You have to know the angular
half angle to calculate the range of indices to use. Once you find the
indices, you sort them to find the order from the exact Bragg condition
(center of disk).

In Mike Kersker's (JEOL, USA) Ph.D. dissertation from , there is a nice
demonstration on how to do this with an ordered alloy phase.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: qxing-at-uno.edu [mailto:qxing-at-uno.edu]
Sent: Thursday, July 21, 2005 8:07 PM
To: Walck-at-SouthBayTech.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by
(qxing-at-uno.edu) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Thursday, July 21, 2005 at 12:25:38
------------------------------------------------------------------------
---

Email: qxing-at-uno.edu
Name: Qingfeng Xing

Organization: University of New Orleans

Education: Graduate College

Location: New Orleans LA 70148

Question: How to index high order Kikuchi lines of silicon?

Clear high order Kikuchi lines can be obtained by CBED at an orientation
far from low-index zones. I found that it's difficult to index them even
knowing the position of the direct beam on the Kickuchi map, as there
are so many lines. Is there any procedure to do that?

Thank you very much.

------------------------------------------------------------------------
---

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From: rbeavers-at-mail.smu.edu
Date: Fri, 22 Jul 2005 12:24:38 -0500
Subject: [Microscopy] Environmental SEM in North Texas

Contents Retrieved from Microscopy Listserver Archives
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Group,

Trying to locate an environmental SEM (meaning I want to look at materials that have liquid solutions as a component) in the North Texas area.

Thanks

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
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From: jpflugheber-at-stlawu.edu
Date: Fri, 22 Jul 2005 13:08:22 -0500
Subject: [Microscopy] Re: Environmental SEM in North Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

See if these guys can help you out:
Molecular and Cellular Imaging Facility
K1 Building
University of Texas Southwestern Medical Center at Dallas
5323 Harry Hines Blvd
Dallas, TX 5390-9039



Office for Christopher Gilpin Ph.D. 214.648.2827

Lab for George Lawton
or Tom Januszewski (214) 648-7291


rbeavers-at-mail.smu.edu wrote:

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} Group,
}
} Trying to locate an environmental SEM (meaning I want to look at materials that have liquid solutions as a component) in the North Texas area.
}
} Thanks
}
} Roy Beavers
}
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, TX 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-smu.edu
}
}
}
} ==============================Original Headers==============================
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8, 27 -- From jpflugheber-at-stlawu.edu Fri Jul 22 13:08:22 2005
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From: jmkrupp-at-cats.ucsc.edu
Date: Fri, 22 Jul 2005 13:45:46 -0500
Subject: [Microscopy] reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

Anybody remember how to reset the computer on a JEOL 1200EX? Its battery
died and it has lost its mind.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



==============================Original Headers==============================
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7, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
7, 17 -- Subject: reset JEOL 1200EX?
7, 17 -- X-UCSC-CATS-MailScanner: Found to be clean
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From: lherault-at-bu.edu
Date: Fri, 22 Jul 2005 13:53:15 -0500
Subject: [Microscopy] reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm sure that once you replace the battery, JEOL will be able to help you
over the phone. They were always very accommodating when we had a JSM 35,
even when it was not under contract.

Ron L

-----Original Message-----
X-from: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Friday, July 22, 2005 2:50 PM
To: lherault-at-bu.edu

Hi:

Anybody remember how to reset the computer on a JEOL 1200EX? Its battery
died and it has lost its mind.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



==============================Original Headers==============================
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7, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
7, 17 -- Subject: reset JEOL 1200EX?
7, 17 -- X-UCSC-CATS-MailScanner: Found to be clean
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==============================Original Headers==============================
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17, 22 -- Subject: RE: [Microscopy] reset JEOL 1200EX?
17, 22 -- Date: Fri, 22 Jul 2005 14:56:44 -0400
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From: jmkrupp-at-cats.ucsc.edu
Date: Fri, 22 Jul 2005 14:17:44 -0500
Subject: [Microscopy] oops, make that a 1200EX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

Anybody remember how to reset the computer on a JEOL 1200EX II? Its battery
died and it has lost its mind.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



==============================Original Headers==============================
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7, 17 -- To: microscopy-at-microscopy.com
7, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
7, 17 -- Subject: oops, make that a 1200EX II
7, 17 -- X-UCSC-CATS-MailScanner: Found to be clean
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From: Tom.Januszewski-at-UTSouthwestern.edu
Date: Fri, 22 Jul 2005 14:19:25 -0500
Subject: [Microscopy] Re: reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jon,
One thing that helps when we get odd characters showing up on our 1200EX
display is to hit control and A simultaneously. This puts an asterisk on
the top of page. Then simply type RESET then hit return.
Let me know if you need a hard reboot. I can give you info on that as well.
Good luck.
Tom

--
Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
Dallas, TX 75390-9039
214-648-7291
214-648-6408 (FAX)
tom.januszewski-at-UTSouthwestern.edu



==============================Original Headers==============================
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From: clei-at-uiuc.edu
Date: Fri, 22 Jul 2005 14:58:54 -0500
Subject: [Microscopy] AskAMicroscopist: How

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The book "Electron microdiffraction" by J.M. Zuo and J. Spence
had such program too.

Ch


---- Original message ----
} Date: Fri, 22 Jul 2005 12:08:48 -0500
} From: walck-at-southbaytech.com
} Subject: [Microscopy] RE: AskAMicroscopist: How to index high
order Kikuchi lines of
} To: clei-at-uiuc.edu
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From: sryazant-at-ucla.edu
Date: Fri, 22 Jul 2005 15:51:30 -0500
Subject: [Microscopy] reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As far as I remember, reset will erase information about pole-piece and
perhaps some other information from the computer memory, so you need to
write it down before resetting. I would try just to turn off-on computer
first (there is switch for it if you open back panel of the right console
(where computer is)). Sergey

At 12:20 PM 7/22/2005, you wrote:



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From: eoptics-at-mcmaster.ca
Date: Fri, 22 Jul 2005 17:36:12 -0500
Subject: [Microscopy] viaWWW: Making Nickel Evaporated Films

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eoptics-at-mcmaster.ca) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, July 22, 2005 at 12:43:58
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Email: eoptics-at-mcmaster.ca
Name: Fred Pearson

Organization: McMaster University

Title-Subject: [Filtered] MListserver: Making Nickel Evaporated Films

Question: Good day

I have been trying to produce films by evaporating nickel onto a glass slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear a very thin soap film across the slide and wipe it until the almost disappears. I then evaporate the nickel. I then score the slide with a sharp needle and immerse it into distilled water at about a 30 degree angle. The film floats in sections. I then scoop up the pieces with 200 um. aluminum grids.

The problem is that when it begins to dry, the film pops within each of the grid squares and disappears.

What I am I doing wrong??? Is the film not thick enough?

I have made other types of films with this exact same procedure with no problems.

thanks in advance

Fred

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From: tivol-at-caltech.edu
Date: Fri, 22 Jul 2005 19:36:16 -0500
Subject: [Microscopy] Re: viaWWW: Making Nickel Evaporated Films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jul 22, 2005, at 3:36 PM, eoptics-at-mcmaster.ca wrote:

} I have been trying to produce films by evaporating nickel onto a glass
} slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear
} a very thin soap film across the slide and wipe it until the almost
} disappears. I then evaporate the nickel. I then score the slide with a
} sharp needle and immerse it into distilled water at about a 30 degree
} angle. The film floats in sections. I then scoop up the pieces with
} 200 um. aluminum grids.
}
} The problem is that when it begins to dry, the film pops within each
} of the grid squares and disappears.
}
} What I am I doing wrong??? Is the film not thick enough?
}
} I have made other types of films with this exact same procedure with
} no problems.
}
Dear Fred,
If the film breaks apart as it is floated off--assuming that is what
you mean by "in sections"--then it may not be structurally strong
enough. You don't say how thick the film is, so I don't have a feeling
whether making it thicker will solve the problem. One thing you could
try is to take the wet grid and put it onto a drop of ethanol, which
will lower the surface tension, and that might reduce the forces that
pop the film. It may be that the evaporated nickel is too crystalline
with relatively weak cohesion between crystals, making it weaker than
might be expected. A possible alternative is to put a formvar coat
onto the grids, evaporate the Ni onto the formvar, then dissolve the
formvar away with CHCl3. If you try this, be careful not to let pools
of CHCl3 touch the grids, but put them on a piece of filter paper
uphill from the CHCl3 and let only the liquid drawn up to the grids
through the paper touch the formvar--the same technique for dissolving
formvar from under a layer of C. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: cgarber-at-2spi.com
Date: Sat, 23 Jul 2005 13:36:46 -0500
Subject: [Microscopy] Making Ni films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fred Pearson wrote:
====================================================
I have been trying to produce films by evaporating nickel onto a glass
slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear a very
thin soap film across the slide and wipe it until the almost disappears. I
then evaporate the nickel. I then score the slide with a sharp needle and
immerse it into distilled water at about a 30 degree angle. The film floats
in sections. I then scoop up the pieces with 200 um. aluminum grids.

The problem is that when it begins to dry, the film pops within each of the
grid squares and disappears.

What I am I doing wrong??? Is the film not thick enough?

I have made other types of films with this exact same procedure with no
problems.
===================================================
You did not mention how you were applying the Ni films, if you are trying to
do this by sputtering, you would need a truly clean and UHV environment or
else, so I have been led to believe, you will end up with a significant
amount of oxide.

My biggest concern would be with the smearing of the "very thin soap film
across the slide". What looks "disappeared" to the eye could be big globs
so far as TEM observation dimensions are concerned (if transferred to the
film) and that could lead to film instability in the electron beam.

An alternative approach might be to evaporate Victawet® (if you are not
familiar with this interesting surfactant, see URL
http://www.2spi.com/catalog/spec_prep/evapor_3c.shtml

By the vacuum evaporation of Victawet, you can apply a more thinner and more
uniform layer than you could ever get by "wiping" and experience has been
that whatever Victawet that might be left (it will dissolve in water) won't
be sensitive in the presence of the electron beam.

We have ourselves not tried Victawet with Ni but have used it quite
successfully with other coating materials and it really does work.

Disclaimer: SPI Supplies is a supplier of Victawet for electron microscopy
so we would have a vested interest in having more people using Victawet.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================








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From: richard-at-torland.demon.co.uk
Date: Sun, 24 Jul 2005 06:07:04 -0500
Subject: [Microscopy] Re: reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

it is quite easy to perform a full reset on a 1200 II. You will however
lose a lot of information, for example type of vacuum system, type of
polepiece and other serious data that the microscope needs to operate
correctly, I would suggest you contact your local Jeol office who can give
instructions over the phone,

Richard Hey

Principal Technical Support Engineer
Jeol (UK) Ltd.



The views expressed herein do not reflect the views of Jeol
























At 15:56 22/07/2005 -0500, you wrote:



} ----------------------------------------------------------------------------
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From: philf-at-newton.umsl.edu
Date: Sun, 24 Jul 2005 10:31:14 -0500
Subject: [Microscopy] viaWWW: Nano-cluster web-lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (philf-at-newton.umsl.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, July 23, 2005 at 14:43:34
---------------------------------------------------------------------------

Email: philf-at-newton.umsl.edu
Name: Phil Fraundorf

Organization: UM-StL Physics and Astronomy/CME

Title-Subject: [Filtered] MListserver: Nano-cluster web-lab

Question: Hi,

We're developing a webpage* for simulation and
analysis of imaging, diffraction, and spectroscopic
data inspired by the fact that for small numbers of
atoms these effects are easier to model. One can
already use it to index cross-fringe lattice
images and single-crystal diffraction patterns
against some candidate structures, and to generate
projected-potential and truncated Debye-sum
diffraction images down any orientation. The latter
allow one to quantify finite-crystal effects,
e.g. of reduced specimen thickness in directions
perpendicular to a g-vector. This gives rise to
high-frequency "fore-shortening tails" in powder
patterns, even as reduced-thickness parallel to
the same g-vector broadens diffraction spots
symmetrically. These effects are quite important
in the study, for example, of data from graphene
nano-structures**.

As far as education is concerned, this virtual
goniometer will offer your students a chance to
take and ponder data from unknown nanostructures,
without the cost of scope time. Moreover, for
those into diffraction, specimen-tilting while
viewing from the side also nicely illustrates a
flat Ewald-sphere in action.

Suggestions and corrections (we're just getting
started) to philf-at-newton.umsl.edu are invited.
Except for this announcement, the system is too
much in its infancy to warrant much bandwidth
on the listserver. However, you might want to
bookmark it, as we hope to develop it into a
reliable resource for research and teaching in days
ahead.

Thanx! /phil

* http://www.umsl.edu/~fraundor/nanowrld/newlive/crystal3.html
** cf. ApJLett 578 (2002) L153 & Warren (1941) PhysRev v59n9p693.

Phil Fraundorf
UM-StL Physics & Astronomy/CME


---------------------------------------------------------------------------

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From: beth-at-plantbio.uga.edu
Date: Mon, 25 Jul 2005 10:29:34 -0500
Subject: [Microscopy] "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ok, so this is not serious journalism but it was in the popular press -
did anyone else read Parade columnist Marilyn vos Savant's article last
Sunday "Are men smarter than women?"? The article is mainly about women
in science or the lack thereof. About half way through the article she
decides to bash microscopists:

"And note that dictators - who aren't any stronger than other men - are
never women. Maybe females just don't have whatever it takes to
bulldoze their way to this dubious sort of "success". No one thinks the
paucity of women in the field of ruthless domination is because they
aren't smart enough! So why should anyone be shocked to find that most
bright people - including women - would flee from the sight of a
microscope?!"

Marilyn is in the Guinness Book of Records Hall of Fame for having the
highest IQ ever recorded. If you feel so inclined to enlighten Marilyn
about microscopy (or bad analogies) you can email her at
{marilyn-at-parade.com} .

see you "not so bright people" in Honolulu,
Beth

PS - she is intelligent enough to say that neither gender is smarter
than the other.

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original Headers==============================
12, 18 -- From beth-at-plantbio.uga.edu Mon Jul 25 10:29:34 2005
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From: hoffpajo-at-yahoo.com
Date: Mon, 25 Jul 2005 10:37:54 -0500
Subject: [Microscopy] Re: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

well this is a good 2 minutes of my life i will never
get back, trume there haven't benn any women
dictators, they just attach themselves to ditators.

--- beth-at-plantbio.uga.edu wrote:

}
}
}
}
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}
} Ok, so this is not serious journalism but it was in
} the popular press -
} did anyone else read Parade columnist Marilyn vos
} Savant's article last
} Sunday "Are men smarter than women?"? The article is
} mainly about women
} in science or the lack thereof. About half way
} through the article she
} decides to bash microscopists:
}
} "And note that dictators - who aren't any stronger
} than other men - are
} never women. Maybe females just don't have whatever
} it takes to
} bulldoze their way to this dubious sort of
} "success". No one thinks the
} paucity of women in the field of ruthless domination
} is because they
} aren't smart enough! So why should anyone be shocked
} to find that most
} bright people - including women - would flee from
} the sight of a
} microscope?!"
}
} Marilyn is in the Guinness Book of Records Hall of
} Fame for having the
} highest IQ ever recorded. If you feel so inclined to
} enlighten Marilyn
} about microscopy (or bad analogies) you can email
} her at
} {marilyn-at-parade.com} .
}
} see you "not so bright people" in Honolulu,
} Beth
}
} PS - she is intelligent enough to say that neither
} gender is smarter
} than the other.
}
}
**********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
} http://www.plantbio.uga.edu/emlab
}
} "Between the two evils,
} I always pick the one I never tried before". Mae
} West (1893-1980)
}
*******************************************************************
}
} "And it's only the giving that makes you what you
} are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
}
************************************************************************
}
} ***
}
}
}
} ==============================Original
} Headers==============================
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} 10:29:34 2005
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} Jul 2005 10:29:33 -0500
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} {beth-at-plantbio.uga.edu}
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From: hinmeigeng-at-hotmail.com
Date: Mon, 25 Jul 2005 15:43:59 -0500
Subject: [Microscopy] ESEM of soil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Any advice on the following?

======
A student working on a Geoarchaeology masters dissertation writes:

I would like to use the environmental SEM soon after
the centre re-opens on the 8th August. I have potentially up to 30 soil and
sediment samples that I am hoping consist of loess to some degree or other.
My plan is to examine the quartz grains in the samples for evidence of
frost-shattering patterns. I've never used this kind of equipment before and
I would very much appreciate any advice you may have regarding preparing
these samples for viewing. Sample size, condition, mounting etc.

======
Thanks for your time,

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



==============================Original Headers==============================
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From: lcgould-at-med.cornell.edu
Date: Mon, 25 Jul 2005 15:52:01 -0500
Subject: [Microscopy] gummy blocks-summary of responses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi and thanks to all of you who responded to my question about gummy
blocks. Here is a summary of the suggestions you sent:

-extra changes of absolute ethanol
-lower temp, longer cure
-more gradations of ethanol-epon mix leading up to pure resin, with
agitation at all steps
-use fresh accelerator ( {6 months old) and switch to BDMA instead of DMP-30
-use HPMA (Hydroypropyl Methacrylate) in place of PO as a final
dehydrating agent, and then stepwise into Epon (it won't eat the
plastic dishes)
-use polypropylene culture dishes
-remove the cover glass and embed in Chang mold

The last 2 suggestions won't work for me. The dishes come in just 1
"flavor" and if I could remove the cover glass without destroying it,
I would have. These are attached with Sylgard (I think), and its a
tight bond.

Thanks again,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: john.mardinly-at-intel.com
Date: Mon, 25 Jul 2005 17:27:55 -0500
Subject: [Microscopy] "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Isn't this the same Marilyn vos Savant who wrote in her column that a
bullet fired from a high powered gun into the air was harmless when it
came back down? I just want to know who did the IQ testing.

John Mardinly
Intel

The opinion of this author is not necessarily the opinion of Intel corp.

-----Original Message-----
X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu]
Sent: Monday, July 25, 2005 8:30 AM
To: Mardinly, John

Ok, so this is not serious journalism but it was in the popular press -

did anyone else read Parade columnist Marilyn vos Savant's article last

Sunday "Are men smarter than women?"? The article is mainly about women

in science or the lack thereof. About half way through the article she
decides to bash microscopists:

"And note that dictators - who aren't any stronger than other men - are

never women. Maybe females just don't have whatever it takes to
bulldoze their way to this dubious sort of "success". No one thinks the

paucity of women in the field of ruthless domination is because they
aren't smart enough! So why should anyone be shocked to find that most
bright people - including women - would flee from the sight of a
microscope?!"

Marilyn is in the Guinness Book of Records Hall of Fame for having the
highest IQ ever recorded. If you feel so inclined to enlighten Marilyn
about microscopy (or bad analogies) you can email her at
{marilyn-at-parade.com} .

see you "not so bright people" in Honolulu,
Beth

PS - she is intelligent enough to say that neither gender is smarter
than the other.

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************

***



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==============================Original Headers==============================
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From: ard-at-ansto.gov.au
Date: Mon, 25 Jul 2005 18:37:26 -0500
Subject: [Microscopy] RE: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well who knows. Perhaps Marilyn has a point about the lack of female
dictators through history. But that's not to say that women haven't
caused just as much damage. Take Helen of Troy for example. (Joke)

Since when was there ever a positive correlation between so-called
intelligence and the abuse of opportunistic bigotry? Marilyn's
mission is to help her employer's media company make money. Thus for
the good of all this sort of crap journalism should just be ignored.

Anyway, aren't IQ tests supposed to be culture and gender biased in
favor of white western males? Has anyone seen a photo of "Marilyn"?


}
} "And note that dictators - who aren't any stronger than other men - are
}
} never women. Maybe females just don't have whatever it takes to
} bulldoze their way to this dubious sort of "success". No one thinks the
}
} paucity of women in the field of ruthless domination is because they
} aren't smart enough! So why should anyone be shocked to find that most
} bright people - including women - would flee from the sight of a
} microscope?!"
}
} Marilyn is in the Guinness Book of Records Hall of Fame for having the
} highest IQ ever recorded. If you feel so inclined to enlighten Marilyn
} about microscopy (or bad analogies) you can email her at
} {marilyn-at-parade.com} .


==============================Original Headers==============================
7, 26 -- From ard-at-ansto.gov.au Mon Jul 25 18:37:23 2005
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From: hoffpajo-at-yahoo.com
Date: Mon, 25 Jul 2005 19:27:32 -0500
Subject: [Microscopy] Re: RE: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

women throughout history have latched themselves on to
men od power, must i mention eva braum.
i am not certain as to why beth would even bring this
up, as far as i can see Marilyn's form of journalism
is self serving crap. i am not even convinced the
letters wrtten to her are real.
so i ask beth, what was your motivation?

--- ard-at-ansto.gov.au wrote:

}
}
}
}
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}
}
} Well who knows. Perhaps Marilyn has a point about
} the lack of female
} dictators through history. But that's not to say
} that women haven't
} caused just as much damage. Take Helen of Troy for
} example. (Joke)
}
} Since when was there ever a positive correlation
} between so-called
} intelligence and the abuse of opportunistic bigotry?
} Marilyn's
} mission is to help her employer's media company make
} money. Thus for
} the good of all this sort of crap journalism should
} just be ignored.
}
} Anyway, aren't IQ tests supposed to be culture and
} gender biased in
} favor of white western males? Has anyone seen a
} photo of "Marilyn"?
}
}
} }
} } "And note that dictators - who aren't any stronger
} than other men - are
} }
} } never women. Maybe females just don't have whatever
} it takes to
} } bulldoze their way to this dubious sort of
} "success". No one thinks the
} }
} } paucity of women in the field of ruthless
} domination is because they
} } aren't smart enough! So why should anyone be
} shocked to find that most
} } bright people - including women - would flee from
} the sight of a
} } microscope?!"
} }
} } Marilyn is in the Guinness Book of Records Hall of
} Fame for having the
} } highest IQ ever recorded. If you feel so inclined
} to enlighten Marilyn
} } about microscopy (or bad analogies) you can email
} her at
} } {marilyn-at-parade.com} .
}
}
} ==============================Original
} Headers==============================
} 7, 26 -- From ard-at-ansto.gov.au Mon Jul 25 18:37:23
} 2005
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} 7, 26 -- Date: Tue, 26 Jul 2005 09:37:17 +1000
} 7, 26 -- To: microscopy-at-microscopy.com
} 7, 26 -- From: Arthur Day {ard-at-ansto.gov.au}
} 7, 26 -- Subject: [Microscopy] RE: "most bright
} people"
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From: zaluzec-at-microscopy.com
Date: Mon, 25 Jul 2005 19:37:05 -0500
Subject: [Microscopy] Administrivia: most bright people

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

This thread is starting to seriously drift off microscopy related themes.
Let's let this one die, before it get too tangential.

Nestor
Your Friendly Neighborhood SysOp

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From: avklaus-at-amnh.org
Date: Mon, 25 Jul 2005 19:39:23 -0500
Subject: [Microscopy] RE: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
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My guess is that Beth thought this was pretty funny and wanted to share it
with her friends and colleagues who she is really looking forward to
seeing next week. If that's the case, I must agree.

Just a guess. I'm looking forward to meeting you Beth!

All best,

Angela
}
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} women throughout history have latched themselves on to
} men od power, must i mention eva braum.
} i am not certain as to why beth would even bring this
} up, as far as i can see Marilyn's form of journalism
} is self serving crap. i am not even convinced the
} letters wrtten to her are real.
} so i ask beth, what was your motivation?
}
} --- ard-at-ansto.gov.au wrote:
}
} }
} }
} }
} }
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} }
} } Well who knows. Perhaps Marilyn has a point about
} } the lack of female
} } dictators through history. But that's not to say
} } that women haven't
} } caused just as much damage. Take Helen of Troy for
} } example. (Joke)
} }
} } Since when was there ever a positive correlation
} } between so-called
} } intelligence and the abuse of opportunistic bigotry?
} } Marilyn's
} } mission is to help her employer's media company make
} } money. Thus for
} } the good of all this sort of crap journalism should
} } just be ignored.
} }
} } Anyway, aren't IQ tests supposed to be culture and
} } gender biased in
} } favor of white western males? Has anyone seen a
} } photo of "Marilyn"?
} }
} }
} } }
} } } "And note that dictators - who aren't any stronger
} } than other men - are
} } }
} } } never women. Maybe females just don't have whatever
} } it takes to
} } } bulldoze their way to this dubious sort of
} } "success". No one thinks the
} } }
} } } paucity of women in the field of ruthless
} } domination is because they
} } } aren't smart enough! So why should anyone be
} } shocked to find that most
} } } bright people - including women - would flee from
} } the sight of a
} } } microscope?!"
} } }
} } } Marilyn is in the Guinness Book of Records Hall of
} } Fame for having the
} } } highest IQ ever recorded. If you feel so inclined
} } to enlighten Marilyn
} } } about microscopy (or bad analogies) you can email
} } her at
} } } {marilyn-at-parade.com} .
} }
} }
} } ==============================Original
} } Headers==============================
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--
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-796-5977
Fax: 212-496-3480


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From: gary-at-gaugler.com
Date: Mon, 25 Jul 2005 19:56:45 -0500
Subject: [Microscopy] Re: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
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Ahhh... well, then I suppose that we won't see her
at M&M 2005. That is too bad. It would be great
to get her enlightened input on EBSD, CL, EDS,
TEM, STEM, DIC, PH, BF, DF, and other EM/LM issues/topics.

I don't see a strong (or any) correlation between IQ
and microscopy. If one is in microscopy, I assume
that this is because they love it. Passion is not IQ.
Focus is not IQ. Some are for a job. But then too,
they do it. And if poorly done, they are done.

OK. So I'm passionate about EM. Get Marilyn here at
M&M 2005 and let's talk about this topic. Otherwise,
relegate her to history. There are way too many other
important topics to worry about then her quantitative IQ.

Regardless of my personal IQ (Intelligence Quotient), my
Interest Quotient (IQ2) for EM is very high. So I would
go face-to-face with her on EM. That would be fun.

gary g.


At 08:34 AM 7/25/2005, you wrote:



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From: liz.girvan-at-stonebow.otago.ac.nz
Date: Mon, 25 Jul 2005 23:41:19 -0500
Subject: [Microscopy] Sputter Coater help for Dunedin!

Contents Retrieved from Microscopy Listserver Archives
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Hi Steve
Hope all is well over there. Great excitement here in SEM land - we
have just got our new Emitech K575X Sputter Coater set up. Allan
suggested I email and see if you had any brilliant idea as to what
kind of test specimen I could use to try it out.
You may also have some advice about how to use properly?
Am still enjoying the Protrain course - getting there slowly!
Thanks for your help
Kind regards
Liz
PS Allan said you're coming down here next July - looking forward to it!
--

------------------------------------------------------------------------------------------------------------------------
Liz Girvan
Electron Microscopist

Otago Centre for Electron Microscopy
C/- Department of Anatomy and Structural Biology
Otago School of Medical Sciences
PO Box 913
Dunedin
New Zealand
Phone (03) 479 7386

http://ocem.otago.ac.nz/

**Ride the Lightning**

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From: innap-at-savion.huji.ac.il
Date: Tue, 26 Jul 2005 01:34:58 -0500
Subject: [Microscopy] ESEM of soil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Robert,
For the beginning you can put a piece of your sample on the standard SEM
metal stub (aroung 12 mm diameter) coated with carbon tape (conductive
carbon glue - also standard for SEM) and use Low Vacuum mode for
studying the morphology of your samples/sediments. When looking at the
micro structural features of the samples and seeing something like
grains, which you can attribute to quartz or can not, you start EDS
acquisitions for measuring their composition. And so on and so on.
First, you will find proper LV conditions for observation (HT, pressure
and spot size) with Gaseous SE detector. Then, in order to separate
heavy and light elements containing features you can use BSE detector.
And then you can measure the composition with EDS. If you want to go up
to the quantification, you have to acquire spectra from the same region
at two different pressure values, because only in this way you will be
able extract a right chemical information.
Good Luck,
Best,
Inna

Dr. Inna Popov
Head of The Unit for Nanoscopic Characterization
The Hebrew University of Jerusalem
E.Safra Campus Givat Ram
Jerusalem 91904
ISRAEL
www.nanoscience.huji.ac.il/unit
Tel: +972 2 6584808
Fax: +972 2 6584809
email: innap-at-savion.huji.ac.il

-----Original Message-----
X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com]
Sent: Monday, July 25, 2005 11:47 PM
To: Inna Popov

Dear Listers,

Any advice on the following?

======
A student working on a Geoarchaeology masters dissertation writes:

I would like to use the environmental SEM soon after
the centre re-opens on the 8th August. I have potentially up to 30 soil
and
sediment samples that I am hoping consist of loess to some degree or
other.
My plan is to examine the quartz grains in the samples for evidence of
frost-shattering patterns. I've never used this kind of equipment before
and
I would very much appreciate any advice you may have regarding preparing
these samples for viewing. Sample size, condition, mounting etc.

======
Thanks for your time,

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



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From: mccaulak-at-wfu.edu
Date: Tue, 26 Jul 2005 08:50:03 -0500
Subject: [Microscopy] viaWWW: user charges for SEM and related equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Liz

How about taking a ~0.25 latex and diluting it until you have an even
monolayer (~30:1 distilled water). Then try coating you should NOT be able
to visualise ANY structure!

You need to remove the chromium oxide prior to applying a coat. Shutter the
target and sputter until the plasma reaches a very nice light blue colour
(once seen never forgotten) then remove the shutter and coat for just a few
seconds.

Good luck as my information is how we ran the prototype so there may be a
better route today?

Regards

Steve

PS see you in July it seems - great

----- Original Message -----
X-from: {liz.girvan-at-stonebow.otago.ac.nz}
To: {protrain-at-emcourses.com}
Sent: Tuesday, July 26, 2005 5:42 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005 at 12:09:42
---------------------------------------------------------------------------

Email: mccaulak-at-wfu.edu
Name: Anita McCauley

Organization: Wake Forest University

Title-Subject: [Filtered] MListserver: user charges for SEM and related equipment

Question: I run a small microscopy facility in which equipment is normally made available to users free of charge. We are currently developing a relationship with an outside user and would like to generate a usage fee list so that we can recover costs for equipment wear and consumables.

Rather than pulling numbers out of thin air, I would like to know what others charge for the use of an SEM, a critical point dryer, and a sputter coater. Tech time does not need to be included. Hopefully with this information, I will be able to put together a reasonable usage fee list.

Thanks.

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:01 2005
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: user charges for SEM and related equipment
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: mccaulak-at-wfu.edu
Date: Tue, 26 Jul 2005 08:50:22 -0500
Subject: [Microscopy] viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005 at 12:19:54
---------------------------------------------------------------------------

Email: mccaulak-at-wfu.edu
Name: Anita McCauley

Organization: Wake Forest University

Title-Subject: [Filtered] MListserver: video camera for stereomicroscope

Question: I am looking to add a video camera to an existing Olympus SZX12 stereomicroscope. I would like to consider cameras ranging from consumer-grade cameras bought at big-box electronic stores to those made for scientific applications. Currently, I am having trouble finding much information regarding scientific-grade video cameras.

I would appreciate hearing from folks that have been using either type of camera on a stereomicroscope, the pros and cons to their set-ups, and whether they are happy with their configuration.

Thanks for the help.

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:22 2005
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6QDoL8n008645
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8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: video camera for stereomicroscope
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: Sven.Terclavers-at-med.kuleuven.be
Date: Tue, 26 Jul 2005 09:14:08 -0500
Subject: [Microscopy] Re: viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Anita,

I've been using two different, commercially available Sony digital
videocameras with success on both Zeiss and Leica stereomicroscopes.
The only disadvantage I experienced is that the cameras have
difficulties when filming samples which have both dark and very light
spots. However, with a little rearrangment of the light setup, things
could be quite solved! It of course also depends on the purpose:
analysis or imaging. But for imagingm the quality is more than enough
to be accepted by top-rated journals!
Best,

Sven Terclavers



Quoting mccaulak-at-wfu.edu:

}
}
}
}
----------------------------------------------------------------------
------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------
------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mccaulak-at-wfu.edu) from
} http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005
at
} 12:19:54
}
----------------------------------------------------------------------
-----
}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: video camera for
} stereomicroscope
}
} Question: I am looking to add a video camera to an existing Olympus
} SZX12 stereomicroscope. I would like to consider cameras ranging
} from consumer-grade cameras bought at big-box electronic stores to
} those made for scientific applications. Currently, I am having
} trouble finding much information regarding scientific-grade video
} cameras.
}
} I would appreciate hearing from folks that have been using either
} type of camera on a stereomicroscope, the pros and cons to their
} set-ups, and whether they are happy with their configuration.
}
} Thanks for the help.
}
}
----------------------------------------------------------------------
-----
}
} ==============================Original
} Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:22 2005
} 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} j6QDoL8n008645
} 8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 08:50:
22
} -0500
} 8, 12 -- Mime-Version: 1.0
} 8, 12 -- X-Sender: (Unverified)
} 8, 12 -- Message-Id: {p06110404bf0bf00e6734-at-[206.69.208.22]}
} 8, 12 -- Date: Tue, 26 Jul 2005 08:50:21 -0500
} 8, 12 -- To: microscopy-at-microscopy.com
} 8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver)
} 8, 12 -- Subject: viaWWW: video camera for stereomicroscope
} 8, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of -
} Headers==============================
}
}




==============================Original Headers==============================
10, 29 -- From Sven.Terclavers-at-med.kuleuven.be Tue Jul 26 09:14:07 2005
10, 29 -- Received: from thumbler.kulnet.kuleuven.ac.be (thumbler.kulnet.kuleuven.ac.be [134.58.240.45])
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10, 29 -- Date: Tue, 26 Jul 2005 16:13:51 +0200
10, 29 -- From: Sven Terclavers {Sven.Terclavers-at-med.kuleuven.be}
10, 29 -- To: microscopy-at-microscopy.com
10, 29 -- Subject: Re: [Microscopy] viaWWW: video camera for stereomicroscope
10, 29 -- References: {200507261353.j6QDrUIE020974-at-ns.microscopy.com}
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==============================End of - Headers==============================




From: skod-at-ises-llc.com
Date: Tue, 26 Jul 2005 10:24:01 -0500
Subject: [Microscopy] Would like to exchange email with experienced Leitz AF users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been in the (possibly foolish) process of restoring a 1991-era
Leitz AMC AF microscope with the intention of building up an automated
image capture/tiling system. Things have been going moderately well, and
most of the system is finally ready to go. However, I've encountered a
curious problem: the microscope controller is not particularly
configurable, and will not release the autofocus unit for normal function
until *after* it has gone completely through its stage homing procedure.
And, since I have changed over to a stepper stage and a more modern
PC-based controller, the old servo-driven stage is no longer even present
in the system.

I have obtained all the documentation that is available from Leitz, and
have been through it with a fine-toothed comb: no mention is ever made of
using only the autofocus as a standalone unit. If anyone has any
experience with hacking on the various mid-90s Leitz AF hardware in any of
its many manifestations (the AMC AF, CDV, LIS, MPV-SP), I'd very much like
to spend a few minutes picking your brain via email or telephone.

My plan B is to simply scrap the AF unit altogether and go with the
digital video based solution offered by the new controller. However, if I
can save myself a few hundred dollars (and justify having purchased the
unit to start with!), I'd like to press the original AF hardware into use
if possible. Many thanks in advance for anyone willing to spend a few
minutes on this topic: please contact me offlist at skod-at-ises-llc.com.

Scott Griffith
ISES-LLC
9745 Steeplechase Drive
Franktown, CO 80116
303-660-2541 voice
303-660-2542 fax
skod-at-ises-llc.com


==============================Original Headers==============================
5, 17 -- From skod-at-ises-llc.com Tue Jul 26 10:24:00 2005
5, 17 -- Received: from trenco2.ises-llc.com ([204.31.68.59])
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5, 17 -- Received: from localhost (thrale.ises-llc.com [192.168.168.10])
5, 17 -- by trenco2.ises-llc.com (8.12.10/8.12.10) with ESMTP id j6QF6f5F021342
5, 17 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:06:41 -0600 (MDT)
5, 17 -- To: microscopy-at-microscopy.com
5, 17 -- Subject: Would like to exchange email with experienced Leitz AF users
5, 17 -- Date: Tue, 26 Jul 2005 09:23:54 -0600
5, 17 -- From: "Scott Griffith" {skod-at-ises-llc.com}
5, 17 -- Organization: ISES-LLC
5, 17 -- Content-Type: text/plain; format=flowed; delsp=yes; charset=iso-8859-1
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5, 17 -- User-Agent: Opera M2(BETA1)/8.02 (MacPPC, build 2129)
==============================End of - Headers==============================




From: amenex-at-amenex.com
Date: Tue, 26 Jul 2005 10:38:02 -0500
Subject: [Microscopy] Re: user charges for SEM and related equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Ms. McCauley:

You asked:
} Question: ... snipppage ... We are currently developing a
} relationship with an outside user and would like to generate
} a usage fee list so that we can recover costs for equipment
} wear and consumables.
}
} Rather than pulling numbers out of thin air, I would like to
} know what others charge for the use of an SEM, a critical point
} dryer, and a sputter coater. Tech time does not need to be
} included. Hopefully with this information, I will be able to
} put together a reasonable usage fee list.

Salaries and overhead ? If you don't recover _all_ costs, then
your charges will be below actual cost to your funding agencies
and therefore unfair to private microscopy labs that have to
make a profit in order to survive in the marketplace.

You need to find out your true costs. Asking others to tell you
the going rates amounts to a combination in restraint of trade
between you and whoever responds or fails to object to this unfair
trade practice. There are still antitrust laws in effect, I think.

Best regards,
George Langford, Sc.D.
Principal Consultant
Amenex Associates, Inc.
http://www.amenex.com/
amenex-at-amenex.com



==============================Original Headers==============================
7, 24 -- From amenex-at-amenex.com Tue Jul 26 10:38:01 2005
7, 24 -- Received: from mail08.voicenet.com (mail08.voicenet.com [207.103.0.34])
7, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j6QFc1iv008629
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7, 24 -- Tue, 26 Jul 2005 11:37:58 -0400 (EDT)
7, 24 -- Date: Tue, 26 Jul 2005 11:37:58 -0400 (EDT)
7, 24 -- Message-Id: {200507261537.j6QFbwf01695-at-email1.voicenet.com}
7, 24 -- X-Authentication-Warning: email1.voicenet.com: georgel set sender to amenex-at-amenex.com using -f
7, 24 -- From: "George Langford, Sc.D." {amenex-at-amenex.com}
7, 24 -- To: "Anita McCauley" {mccaulak-at-wfu.edu}
7, 24 -- CC: microscopy-at-microscopy.com, amenex-at-amenex.com
7, 24 -- Subject: Re: user charges for SEM and related equipment
7, 24 -- References:
7, 24 -- In-Reply-To:
7, 24 -- X-Mailer: Voicenet Webmail
7, 24 -- X-IPAddress: 67.100.47.123
7, 24 -- X-Sender: georgel-at-voicenet.com
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==============================End of - Headers==============================




From: mccaulak-at-wfu.edu
Date: Tue, 26 Jul 2005 10:56:57 -0500
Subject: [Microscopy] Re: viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for the feedback...I have been looking at some Sony MiniDVs and so
its good to hear that folks have been able to use them successfully.

Anita K. McCauley, Ph.D.
Director of Microscopy/Adj. Asst. Prof.
PO Box 7325
Biology Department
Wake Forest University
Winston-Salem, NC 27109


-----Original Message-----
X-from: Sven.Terclavers-at-med.kuleuven.be
[mailto:Sven.Terclavers-at-med.kuleuven.be]
Sent: Tuesday, July 26, 2005 10:17 AM
To: mccaulak-at-wfu.edu

Dear Anita,

I've been using two different, commercially available Sony digital
videocameras with success on both Zeiss and Leica stereomicroscopes.
The only disadvantage I experienced is that the cameras have
difficulties when filming samples which have both dark and very light
spots. However, with a little rearrangment of the light setup, things
could be quite solved! It of course also depends on the purpose:
analysis or imaging. But for imagingm the quality is more than enough
to be accepted by top-rated journals!
Best,

Sven Terclavers



Quoting mccaulak-at-wfu.edu:

}
}
}
}
----------------------------------------------------------------------
------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------
------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mccaulak-at-wfu.edu) from
} http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005
at
} 12:19:54
}
----------------------------------------------------------------------
-----
}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: video camera for
} stereomicroscope
}
} Question: I am looking to add a video camera to an existing Olympus
} SZX12 stereomicroscope. I would like to consider cameras ranging
} from consumer-grade cameras bought at big-box electronic stores to
} those made for scientific applications. Currently, I am having
} trouble finding much information regarding scientific-grade video
} cameras.
}
} I would appreciate hearing from folks that have been using either
} type of camera on a stereomicroscope, the pros and cons to their
} set-ups, and whether they are happy with their configuration.
}
} Thanks for the help.
}
}
----------------------------------------------------------------------
-----
}
} ==============================Original
} Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:22 2005
} 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} j6QDoL8n008645
} 8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 08:50:
22
} -0500
} 8, 12 -- Mime-Version: 1.0
} 8, 12 -- X-Sender: (Unverified)
} 8, 12 -- Message-Id: {p06110404bf0bf00e6734-at-[206.69.208.22]}
} 8, 12 -- Date: Tue, 26 Jul 2005 08:50:21 -0500
} 8, 12 -- To: microscopy-at-microscopy.com
} 8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver)
} 8, 12 -- Subject: viaWWW: video camera for stereomicroscope
} 8, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of -
} Headers==============================
}
}




==============================Original Headers==============================
10, 29 -- From Sven.Terclavers-at-med.kuleuven.be Tue Jul 26 09:14:07 2005
10, 29 -- Received: from thumbler.kulnet.kuleuven.ac.be
(thumbler.kulnet.kuleuven.ac.be [134.58.240.45])
10, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
j6QEE6tS023423
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-0500
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+0200 (CEST)
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From: GBurgess-at-exchange.hsc.mb.ca
Date: Tue, 26 Jul 2005 11:01:10 -0500
Subject: [Microscopy] saturated NaOH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm trying to express in grams the amount of NaOH that I'd need for 100 ml
of saturated NaOH in ethanol.

Does anyone know this off the top of their head without me having to find a
reference or figure it out with the real pellets.

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

==============================Original Headers==============================
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From: dsherman-at-purdue.edu
Date: Tue, 26 Jul 2005 11:07:59 -0500
Subject: [Microscopy] Re: viaWWW: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anita,

You do have to be careful about this topic on the list. There is a concern
(rightly so) from for-profit companies that Universities will deprive them
of business by undercutting fees. The question you ask is fairly complex.
You do need to look at all costs associated with your facility (service
contracts, consumables, telephone, etc) and come up with a rate structure
that would cover these costs if you needed to charge all current users.

Then look at your internal users as subsidized. To the base costs, add the
percentage used by your university for indirect costs. This would be the
same amount charged to grants to cover building costs, lights, heating, etc.
This can be as high as 50% in many institutions.

If you are doing the work than figure your salary, fringe benefits, etc. to
get an hourly rate that compensates totally for your time.
Tack on a few extra dollars when figuring consumable costs as prices go up
regularly and you need to recoup these costs without having to change your
whole rate structure.

For example: Assume an SEM with $15,000 service contract and consumables
(filaments, nitrogen, etc) of $2000/yr. Figure 1000 hours of billable use
time results in an hourly charge of $17/hr. Add time you spend maintaining
the instrument (aligning, changing filaments, other non-billable time adding
up to 1000hrs/yr) and this figure can easily double. Add the 50% overhead
and you arrive at $51/hr.

This amount is very low compared to what for-profit labs must charge as they
have to use capital to purchase the instrument in the first place and then
depreciate it so they will have funds to replace when necessary. They also
need to pay all costs associated with the physical structures, advertising,
etc. This is why they are concerned about Universities undercutting their
necessary charges. If you have no private labs near you than you can
probably get away with the lower costs. Industry would be delighted with
this amount/hr as their costs if they owned the machine would be much
greater due to the depreciation, etc.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



On 7/26/05 8:52 AM, "mccaulak-at-wfu.edu" {mccaulak-at-wfu.edu} wrote:

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} ---------------------------------------------------------------------------
}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: user charges for SEM and related
} equipment
}
} Question: I run a small microscopy facility in which equipment is normally
} made available to users free of charge. We are currently developing a
} relationship with an outside user and would like to generate a usage fee list
} so that we can recover costs for equipment wear and consumables.
}
} Rather than pulling numbers out of thin air, I would like to know what others
} charge for the use of an SEM, a critical point dryer, and a sputter coater.
} Tech time does not need to be included. Hopefully with this information, I
} will be able to put together a reasonable usage fee list.
}
} Thanks.
}
} ---------------------------------------------------------------------------
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} ==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Tue, 26 Jul 2005 11:12:34 -0500
Subject: [Microscopy] Re: saturated NaOH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

as i recall 52 gms of NaOH in 100ml DH20 will yield a
saturated soln
john

--- GBurgess-at-exchange.hsc.mb.ca wrote:

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}
} I'm trying to express in grams the amount of NaOH
} that I'd need for 100 ml
} of saturated NaOH in ethanol.
}
} Does anyone know this off the top of their head
} without me having to find a
} reference or figure it out with the real pellets.
}
} This e-mail and/or any documents in this
} transmission is intended for the address(s) only and
} may contain legally privileged or confidential
} information. Any unauthorized use, disclosure,
} distribution, copying or dissemination is strictly
} prohibited. If you receive this transmission in
} error, please notify the sender immediately and
} return the original.
}
} ==============================Original
} Headers==============================
} 4, 19 -- From GBurgess-at-exchange.hsc.mb.ca Tue Jul 26
} 11:01:10 2005
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} 4, 19 -- Date: Tue, 26 Jul 2005 10:59:25 -0500
} 4, 19 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
} 4, 19 -- Subject: saturated NaOH
} 4, 19 -- To: "'microscopy-at-microscopy.com'"
} {microscopy-at-microscopy.com}
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From: bfoster-at-mme1.com
Date: Tue, 26 Jul 2005 11:35:33 -0500
Subject: [Microscopy] Re: viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Anita

Also, contact Bill Miller at microbill-at-mohawk.net or by phone at (860)672-0068.

Just a reminder: remember that what makes a stereo microscope stereo is your looking with two eyes and that a camera will only "look" with one. You may need to adjust the lighting to make your objects look a little more 3D.

Good hunting!

Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.

At 10:59 AM 7/26/2005, mccaulak-at-wfu.edu wrote:


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From: edelmare-at-muohio.edu
Date: Tue, 26 Jul 2005 11:45:32 -0500
Subject: [Microscopy] Re: viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anita:

"Consumer-grade" cameras these have built in non-removable
lenses. Unless you are really straped for money you want a camera
which makes use of the steroscope's optics (for which you spent
alot more than the $0.5-$10 piece of plastic sitting on a consumer
grade camera). In which case you are now looking for a C-mount
camera.

Since you have the SZX-12 I would defintiely recommend
getting a U-TV0.5XC-2; CCD CAMERA ADAPTER, for mounting
a c-mount "video camera". It is very compact, and has built in
parfocalizing optics (for matching the eye pieces to the camera
view) which you set and forget. (You will have to determine if you
need to add other compoonents to get a photo port on your SZX12.
If you must go through the eye-piece for cost reasons there are
some very nice video couplers for this. E.g. Thales Optem
optemintl.com or Diagnostic Instruments www.diaginc.com )

Next up for "video camera" becomes what do you want to do
with it? What do you mean by "video camera"? Do you truely mean
"video" as in television, VCR's, "live" motion video?

Standard NTSC (north american television) show you can
display it on a "TV monitor", or do you want to use a computer to
capture images? A tv monitor will require an analog input (or DV
input), and you will need a capture card (Grabber) or a digital
camera output (DV or IEEE-1394/firewire, etc.) to convert this to
something a computer can use.

Still images or motion analysis? Still images moves into "digtal
cameras" but motion anaylsis starts dealing with "frame" rates (for
normal TV-monitors you need Standard NTSC = 30 frames/sec
and PAL 25 frames/sec - yes, interlaced).

Monochrome or color?

Then you get into resolution standard NTSC is 640x480pixels
good and inexpensive for TV monitors but not very good for
"publication". Higher resolution 1024 x 768 or 1280 x 980 or HDTV

Low light (fluorescence) vs normal bright-field / reflected light
work?

Hmm, looking around I found www.theimagingsource.com and
it has lots of c-mount camera choices and pricing! (I have never
shoped there yet but it does look very interesting) There are lots of
other sources for "video" camera out there. and pricing will range
from $300 to $5,000 on up into pricing over $200,000. I am sure
you will here from a few vendors shortly.

In video cameras we here have a Dage MTI camera, two Sony
cameras, and a Javalin camera collected through the years (none
of which are available any longer)

Good luck and feel free to contact me back with more
questions.

Note: We're a univeristy EM Faciltiy I do not sell any video
equipment nor hold any financial interests in any company
mentioned above . . . at least I don't think I do.

On 26 Jul 2005, at 8:51, mccaulak-at-wfu.edu wrote:

} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: video camera for stereomicroscope
}
} Question: I am looking to add a video camera to an existing
} Olympus SZX12 stereomicroscope. I would like to consider cameras
} ranging from consumer-grade cameras bought at big-box electronic
} stores to those made for scientific applications. Currently, I am
} having trouble finding much information regarding scientific-grade
} video cameras.
}
} I would appreciate hearing from folks that have been using either
} type of camera on a stereomicroscope, the pros and cons to their
} set-ups, and whether they are happy with their configuration.
}
} Thanks for the help.
}
} ---------------------------------------------------------------------------


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."

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From: donc-at-asmicro.com
Date: Tue, 26 Jul 2005 11:49:55 -0500
Subject: [Microscopy] Re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debby Sherman wrote "If you have no private labs near you than you can
probably get away with the lower [prices]." I've always been concerned about
the fuzzy definition of "near-ness". My AFM analytical services business in
Indianapolis, Indiana has a worldwide clientele because express courier
services extend our reach. This is fortunate, for if I had to depend on the
business generated within the state of Indiana I would have gone out of
business many years ago.

George Langford comments that a survey of going rates might violate
antitrust laws. Nevertheless, I think the policy of government granting
agencies requires this. Remember: no one ever said the government has to
be self-consistent.

The unfortunate aspect of university services is that they really are
driving the commercial labs out of business. I can say with confidence that
the number of commercial, for-profit labs providing AFM service in the US
has declined significantly in the last few years. I know this because I
have been buying their AFM equipment for resale.

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]

----- Original Message -----
From: dsherman-at-purdue.edu
To: donc-at-asmicro.com
Sent: Tuesday, July 26, 2005 11:11 AM
Subject: [a] [Microscopy] Re: viaWWW: user charges for SEM and related





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Anita,

You do have to be careful about this topic on the list. There is a
concern
(rightly so) from for-profit companies that Universities will deprive them
of business by undercutting fees. The question you ask is fairly complex.
You do need to look at all costs associated with your facility (service
contracts, consumables, telephone, etc) and come up with a rate structure
that would cover these costs if you needed to charge all current users.

Then look at your internal users as subsidized. To the base costs, add
the
percentage used by your university for indirect costs. This would be the
same amount charged to grants to cover building costs, lights, heating,
etc.
This can be as high as 50% in many institutions.

If you are doing the work than figure your salary, fringe benefits, etc.
to
get an hourly rate that compensates totally for your time.
Tack on a few extra dollars when figuring consumable costs as prices go up
regularly and you need to recoup these costs without having to change your
whole rate structure.

For example: Assume an SEM with $15,000 service contract and consumables
(filaments, nitrogen, etc) of $2000/yr. Figure 1000 hours of billable use
time results in an hourly charge of $17/hr. Add time you spend
maintaining
the instrument (aligning, changing filaments, other non-billable time
adding
up to 1000hrs/yr) and this figure can easily double. Add the 50% overhead
and you arrive at $51/hr.

This amount is very low compared to what for-profit labs must charge as
they
have to use capital to purchase the instrument in the first place and then
depreciate it so they will have funds to replace when necessary. They
also
need to pay all costs associated with the physical structures,
advertising,
etc. This is why they are concerned about Universities undercutting their
necessary charges. If you have no private labs near you than you can
probably get away with the lower costs. Industry would be delighted with
this amount/hr as their costs if they owned the machine would be much
greater due to the depreciation, etc.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



On 7/26/05 8:52 AM, "mccaulak-at-wfu.edu" {mccaulak-at-wfu.edu} wrote:

}
}
}


} --------------------------------------------------------------------------
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}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted
} by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on
} Monday, July 25, 2005 at 12:09:42


} --------------------------------------------------------------------------
-
}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: user charges for SEM and related
} equipment
}
} Question: I run a small microscopy facility in which equipment is
normally
} made available to users free of charge. We are currently developing a
} relationship with an outside user and would like to generate a usage fee
list
} so that we can recover costs for equipment wear and consumables.
}
} Rather than pulling numbers out of thin air, I would like to know what
others
} charge for the use of an SEM, a critical point dryer, and a sputter
coater.
} Tech time does not need to be included. Hopefully with this
information, I
} will be able to put together a reasonable usage fee list.
}
} Thanks.
}


} --------------------------------------------------------------------------
-
}
} ==============================Original
Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:01 2005
} 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com
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} 8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver)
} 8, 12 -- Subject: viaWWW: user charges for SEM and related equipment
} 8, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of -
Headers==============================



==============================Original
Headers==============================
13, 21 -- From dsherman-at-purdue.edu Tue Jul 26 11:07:59 2005
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(mailhub131.itcs.purdue.edu [128.210.5.131])
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13, 21 -- Date: Tue, 26 Jul 2005 11:07:56 -0500
13, 21 -- Subject: Re: [Microscopy] viaWWW: user charges for SEM and
related
13, 21 -- equipment
13, 21 -- From: Debby Sherman {dsherman-at-purdue.edu}
13, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
13, 21 -- Message-ID: {BF0BCA0C.7850%dsherman-at-purdue.edu}
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==============================Original Headers==============================
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35, 21 -- References: {200507261611.j6QGBI4o006065-at-ns.microscopy.com}
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From: beth-at-plantbio.uga.edu
Date: Tue, 26 Jul 2005 11:51:32 -0500
Subject: [Microscopy] sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I apologize to the list for posting Marilyn vos Savant's statement on
microscopy. I just wanted to share the article because I thought it was
humorous...I shall refrain from such postings.
best,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original Headers==============================
8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26 11:51:32 2005
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From: pgrover-at-bilbo.bio.purdue.edu
Date: Tue, 26 Jul 2005 11:54:45 -0500
Subject: [Microscopy] Re: user charges for SEM and related equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

George and Debby are correct. It is not fair to independent (=non taxpayer
subsidized) labs when a university facility charges artificially low fees
for instruments bought with public money, and it is actually a violation of
federal law for them to compete in this manner. I remember that in the '70s
Okla. State University's 'dairy barn' had to stop selling dairy products at
prices below those of local grocery stores. However more recently I
couldn't get business for my own SEM services because our Lafayette, IN
industries tell me they can get their work done at our local university for
little or no cost. But might as well mention the illegality of this
practice to a brick wall. Grrrrrrrr.

Paul Grover
Erstwhile Chief Microscopist & Bottle Washer
Microvista Laboratory


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln




==============================Original Headers==============================
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6, 24 -- Subject: [Microscopy] Re: user charges for SEM and related equipment
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From: hoffpajo-at-yahoo.com
Date: Tue, 26 Jul 2005 12:05:04 -0500
Subject: [Microscopy] Re: sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

eh go ahead and post, some of us got a chuckle out of
it

--- beth-at-plantbio.uga.edu wrote:

}
}
}
}
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} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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}
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}
} I apologize to the list for posting Marilyn vos
} Savant's statement on
} microscopy. I just wanted to share the article
} because I thought it was
} humorous...I shall refrain from such postings.
} best,
} Beth
}
}
**********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
} http://www.plantbio.uga.edu/emlab
}
} "Between the two evils,
} I always pick the one I never tried before". Mae
} West (1893-1980)
}
*******************************************************************
}
} "And it's only the giving that makes you what you
} are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
}
************************************************************************
}
} ***
}
}
}
} ==============================Original
} Headers==============================
} 8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26
} 11:51:32 2005
} 8, 18 -- Received: from dogwood.plantbio.uga.edu
} (dogwood.plantbio.uga.edu [128.192.26.2])
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} ESMTP id j6QGpVhb000423
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} Jul 2005 11:51:31 -0500
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} DES-CBC3-SHA (168 bits))
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} {beth-at-plantbio.uga.edu}
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} ==============================End of -
} Headers==============================
}




____________________________________________________
Start your day with Yahoo! - make it your home page
http://www.yahoo.com/r/hs


==============================Original Headers==============================
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6, 19 -- Subject: Re: [Microscopy] sorry about that
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From: dmclea-at-sandia.gov
Date: Tue, 26 Jul 2005 12:08:09 -0500
Subject: [Microscopy] sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Beth,

We must remember, that Microscopists do not live by electrons alone...a
little levity is ALWAYS WELCOME! Especially at the National Labs!

Dorrance McLean
Sandia National Laboratories
Livermore, CA

-----Original Message-----
X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu]
Sent: Tuesday, July 26, 2005 9:52 AM
To: McLean, Dorrance

I apologize to the list for posting Marilyn vos Savant's statement on
microscopy. I just wanted to share the article because I thought it was
humorous...I shall refrain from such postings.
best,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original
Headers==============================
8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26 11:51:32 2005
8, 18 -- Received: from dogwood.plantbio.uga.edu
(dogwood.plantbio.uga.edu [128.192.26.2])
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11:51:31 -0500
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8, 18 -- Subject: sorry about that
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8, 18 -- To: microscopy {microscopy-at-microscopy.com}
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From: gwe-at-ufl.edu
Date: Tue, 26 Jul 2005 12:08:12 -0500
Subject: [Microscopy] M&M 2005 Attendees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If you have any announcements to be included in the daily newsleter of
the meeting, please email me before noon (EST) on Friday or drop your
copy by the LAC Headquarters at the convention center.

Thanks,
Greg

--
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
Phone: 352-392-1295
Fax: 352 846 0251



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From: granta-at-geol.queensu.ca
Date: Tue, 26 Jul 2005 12:19:39 -0500
Subject: [Microscopy] Re: sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don't apologize - it was a humorous (and brief) posting. (I noticed the
complainers were mostly men- significant??).
Keep 'em coming!

Alan Grant
Dept. of Geological Sciences and Geological Engineering
Queen's University
Kingston ON
Canada K7L 3N6


beth-at-plantbio.uga.edu wrote:

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==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Tue, 26 Jul 2005 12:24:27 -0500
Subject: [Microscopy] sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i didn't complain just pointed out a few things that
were left out.

--- granta-at-geol.queensu.ca wrote:

}
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}
} Don't apologize - it was a humorous (and brief)
} posting. (I noticed the
} complainers were mostly men- significant??).
} Keep 'em coming!
}
} Alan Grant
} Dept. of Geological Sciences and Geological
} Engineering
} Queen's University
} Kingston ON
} Canada K7L 3N6
}
}
} beth-at-plantbio.uga.edu wrote:
}
}
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} }
} } I apologize to the list for posting Marilyn vos
} Savant's statement on
} } microscopy. I just wanted to share the article
} because I thought it was
} } humorous...I shall refrain from such postings.
} } best,
} } Beth
} }
}
} **********************************************************************
} } Beth Richardson
} } EM Lab Coordinator
} } Plant Biology Department
} } University of Georgia
} } Athens, GA 30602-7271
} }
} } Phone - (706) 542-1790 & FAX - (706) 542-1805
} } http://www.plantbio.uga.edu/emlab
} }
} } "Between the two evils,
} } I always pick the one I never tried before". Mae
} West (1893-1980)
}
} *******************************************************************
} }
} } "And it's only the giving that makes you what you
} are".
} } Wond'ring Aloud, Jethro Tull (Aqualung)
} }
}
} ************************************************************************
}
} } ***
} }
} }
} }
} } ==============================Original
} Headers==============================
} } 8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26
} 11:51:32 2005
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} }
} }
}
} ==============================Original
} Headers==============================
} 5, 19 -- From granta-at-geol.queensu.ca Tue Jul 26
} 12:19:39 2005
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____________________________________________________
Start your day with Yahoo! - make it your home page
http://www.yahoo.com/r/hs


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From: maureen_petersen-at-msn.com
Date: Tue, 26 Jul 2005 15:35:38 -0500
Subject: [Microscopy] RE: sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Beth: I noticed that a critical comment about this (took too much of his
time?) took the time to post two times on the topic. I think some people
take themselves too seriously.

Maureen Petersen
Duke Univ.

} From: beth-at-plantbio.uga.edu
} Reply-To: microscopy-at-microscopy.com
} To: Maureen_Petersen-at-msn.com
} Subject: [Microscopy] sorry about that
} Date: Tue, 26 Jul 2005 11:52:00 -0500
}
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From: sryazant-at-ucla.edu
Date: Tue, 26 Jul 2005 15:35:54 -0500
Subject: [Microscopy] Re: sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don't be sorry: it was good posting and we need something humorous here
from time to time. Sergey

At 09:53 AM 7/26/2005, you wrote:



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From: chiphead-at-sbcglobal.net
Date: Tue, 26 Jul 2005 15:58:51 -0500
Subject: [Microscopy] Re: Identifications, Courtesy etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


And I wouldn't take much (any) notice of postings by anyone who doesn't identify
themselves but who hides behind a freebie email address

cheers

rtch



Date sent: Tue, 26 Jul 2005 15:37:50 -0500
To: r.sims-at-auckland.ac.nz
X-from: maureen_petersen-at-msn.com
Send reply to: microscopy-at-microscopy.com

--- r.sims-at-auckland.ac.nz wrote:
} And I wouldn't take much (any) notice of postings by
} anyone who doesn't identify
} themselves but who hides behind a freebie email
} address
}
} cheers
}
} rtch

If this were a totally "private" list, I would agree
with you. But given that it is realatively open, and
given the current climate, from a corporate, identity
theft, legal "this opinion is not necess....", etc., I
don't know that it's fair to just write off a poster
because they choose not to be fully identified.

John W. Raffensperger, Jr.
Beaver Dam, Wisconsin, USofA


==============================Original Headers==============================
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From: avklaus-at-amnh.org
Date: Tue, 26 Jul 2005 16:23:40 -0500
Subject: [Microscopy] sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ditto. It was funny and appreciated. I shared it with several friends.
Thanks for posting. -Angela

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} Don't be sorry: it was good posting and we need something humorous here
} from time to time. Sergey
}
} At 09:53 AM 7/26/2005, you wrote:
}
}
}
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} } America


--
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-796-5977
Fax: 212-496-3480


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From: zaluzec-at-microscopy.com
Date: Tue, 26 Jul 2005 17:49:32 -0500
Subject: [Microscopy] Administrivia: sorry about that/most bright people

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Beth & everyone else

There was no problem with Beth's initial posting and no apology
was needed here Beth. Sorry if I appeared to have come down on you.

The followups IMHO were starting to drift into critiques of Marilyn whomever
and her "crap Journalism" and I was trying to nip that direction of
critique in the bud. However, I see I had the opposite effect. Oh well.....

As Dorrance said, everyone can use a grin occassionally .


Nestor






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==============================Original Headers==============================
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From: sryazant-at-ucla.edu
Date: Tue, 26 Jul 2005 18:40:35 -0500
Subject: [Microscopy] Re: Administrivia: sorry about that/most bright

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor
I don't see any reason why we could not discuss the quality of somebody's
work even if it's a journalist with former (?) high IQ. Is it prohibited
to discuss people's work with high IQ on this ListServer? Sergey

At 03:51 PM 7/26/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
and UCLA Institute of Nanotechnology
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095


Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu




==============================Original Headers==============================
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From: cornheadorama-at-hotmail.com
Date: Tue, 26 Jul 2005 18:46:47 -0500
Subject: [Microscopy] viaWWW: Looking for limited TAS(+?) motor wiring information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, July 26, 2005 at 18:41:08
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: Techutopia

Title-Subject: [Filtered] Looking for limited TAS(+?) motor wiring information.

Question: Hi,

I have a Leitz Orthoplan microscope from a TAS (or is it TAS+?) system. It has motorized stage and fine focus, relay lenses etc. for video.

I didn't pick up the console, controller, or schematics. I'm getting pretty handy with LabVIEW machine vision and motion control, so I'd like to revive this scope around a PC platform.

I need limited wiring diagram information. In particular, I need pinouts for the stepper motors, or to figure out the pinouts indirectly from other elements of the schematic. Ratings or replacement part information for the motors might be helpfull too, barring that I may be able to approximate these by studying the schematic in more detail.

If you have any wiring information for these things, please let me know.

} From the number of pins present, it appears as though the X and Y drives may have encoders associated with them, wheras focus deson't. I don't have the image rotation prism option, and in fact I'd be passively interested in buying one (image roatation is pretty trivial in SW these days).

I'd also be willing to part with the 'scope as opposed to undertaking the project if you're a big fan, and if I do proceed with the PC retrofit I may be offering the whole package after I've played with it for a while. The real motivation for having it evaporated soon after I bought it several years ago.

I can be reached at cornheadorama-at-hotmail.com

-Jeff

---------------------------------------------------------------------------

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14, 12 -- Subject: viaWWW: Looking for limited TAS(+?) motor wiring information
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From: zaluzec-at-microscopy.com
Date: Tue, 26 Jul 2005 19:09:12 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Person

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sergey

A honest discussion or even disagreement about a subject or work which is microscopy
related (even tangentially) is not prohibited.

However, it is against the rules to intentionally disparage any person
or organization on the Listserver. This rule applies equally to Journalists as well
as Microscopists. Please review the rules which you received upon subscription.


http://microscopy.com/MicroscopyListserver/Rules.html

Presumably a person of hi IQ has the ability to distinguish between these two.

Nestor
Your Friendly Neighborhood SysOp







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==============================Original Headers==============================
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15, 13 -- To: microscopy-at-microscopy.com
15, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
15, 13 -- Subject: Discussion of high IQ vs Disparagement of a Person
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From: sryazant-at-ucla.edu
Date: Tue, 26 Jul 2005 20:26:30 -0500
Subject: [Microscopy] Re: Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My IQ is very low, Nestor, so I am electron microscopist for last 30
years... Is against rules to say truth? You know, truth sometime hurts
and may be politically incorrect... This forum becomes too much politically
correct and heavily censured (yes, it's true), so I am loosing interest
here... I had enough censorship and "rules" in USSR. But, I have to admit
US is over performing USSR now, so I feel I am at home. Sergey


At 05:10 PM 7/26/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
and UCLA Institute of Nanotechnology
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095


Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu




==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Tue, 26 Jul 2005 20:30:52 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HERE HERE well said

--- sryazant-at-ucla.edu wrote:

}
}
}
}
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} Microscopy Society of America
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}
} My IQ is very low, Nestor, so I am electron
} microscopist for last 30
} years... Is against rules to say truth? You know,
} truth sometime hurts
} and may be politically incorrect... This forum
} becomes too much politically
} correct and heavily censured (yes, it's true), so I
} am loosing interest
} here... I had enough censorship and "rules" in
} USSR. But, I have to admit
} US is over performing USSR now, so I feel I am at
} home. Sergey
}
}
} At 05:10 PM 7/26/2005, you wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} ----------------------------------------------------------------------------
} }
} } Sergey
} }
} } A honest discussion or even disagreement about a
} subject or work which
} } is microscopy
} } related (even tangentially) is not prohibited.
} }
} } However, it is against the rules to intentionally
} disparage any person
} } or organization on the Listserver. This rule
} applies equally to
} } Journalists as well
} } as Microscopists. Please review the rules which
} you received
} } upon subscription.
} }
} }
}
} http://microscopy.com/MicroscopyListserver/Rules.html
} }
} } Presumably a person of hi IQ has the ability to
} distinguish between these
} } two.
} }
} } Nestor
} } Your Friendly Neighborhood SysOp
} }
} }
} }
} }
} }
} }
} }
} }
}
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} ----------------------------------------------------------------------------
} } }
} } } Nestor
} } } I don't see any reason why we could not discuss
} the quality of somebody's
} } } work even if it's a journalist with former (?)
} high IQ. Is it prohibited
} } } to discuss people's work with high IQ on this
} ListServer? Sergey
} } }
} } } At 03:51 PM 7/26/2005, you wrote:
} } }
} } }
} } }
} }
}
} } ------------------------------------------------------------------------
}
} } ----
} } } } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} } ------------------------------------------------------------------------
}
} } ----
} } } }
} } } } Beth & everyone else
} } } }
} } } } There was no problem with Beth's initial
} posting and no apology
} } } } was needed here Beth. Sorry if I appeared to
} have come down on you.
} } } }
} } } } The followups IMHO were starting to drift into
} critiques of Marilyn
} } whomever
} } } } and her "crap Journalism" and I was trying to
} nip that direction of
} } } } critique in the bud. However, I see I had the
} opposite effect. Oh well.....
} } } }
} } } } As Dorrance said, everyone can use a grin
} occassionally .
} } } }
} } } }
} } } } Nestor
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
}
} ----------------------------------------------------------------------
}
} } ------
} } } } } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of
} } America
} } } } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } } } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
}
} ----------------------------------------------------------------------
}
} } ------
} } } } }
} } } } } I apologize to the list for posting Marilyn
} vos Savant's statement on
} } } } } microscopy. I just wanted to share the article
} because I thought it was
} } } } } humorous...I shall refrain from such postings.
} } } } } best,
} } } } } Beth
} } } } }
} } } }
}
} **********************************************************************
} } } } } Beth Richardson
} } } } } EM Lab Coordinator
} } } } } Plant Biology Department
} } } } } University of Georgia
} } } } } Athens, GA 30602-7271
} } } } }
} } } } } Phone - (706) 542-1790 & FAX - (706)
} 542-1805
} } } } } http://www.plantbio.uga.edu/emlab
} } } } }
} } } } } "Between the two evils,
} } } } } I always pick the one I never tried before".
} Mae West (1893-1980)
} } } }
}
} *******************************************************************
} } } } }
} } } } } "And it's only the giving that makes you what
} you are".
} } } } } Wond'ring Aloud, Jethro Tull (Aqualung)
} } } } }
} } } }
}
} ************************************************************************
} } } } } ***
} } } } }
} } } } }
} } } } }
} } } } } ==============================Original
} } Headers==============================
} } } } } 8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26
} 11:51:32 2005
} } } } } 8, 18 -- Received: from
} dogwood.plantbio.uga.edu
} } } } (dogwood.plantbio.uga.edu [128.192.26.2])
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} (8.12.11/8.12.8) with ESMTP id
} } } } j6QGpVhb000423
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} {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005
} } } } 11:51:31 -0500
} } } } } 8, 18 -- Received: from localhost
} ([127.0.0.1])
}
=== message truncated ===




____________________________________________________
Start your day with Yahoo! - make it your home page
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From: cgarber-at-2spi.com
Date: Tue, 26 Jul 2005 21:51:09 -0500
Subject: [Microscopy] Apology to the list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Great posting. One has to wonder about the role model of ethical values when
one bases their decisions on the basis of what they can "get away " with.

You probably think I am losing my balls for keeping silent. I almost
composed a posting but then again, I did not want to antagonize some of my
best customers. Which is of course exactly what I woudl be doing.

Maybe I will have some scotch and get up the nerve to create a posting.
Question: Will we see you in Honolulu?

Chuck
----- Original Message -----
X-from: {donc-at-asmicro.com}
To: {cgarber-at-2spi.com}
Sent: Tuesday, July 26, 2005 12:52 PM

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Please accept my apologies for inadvertently sending out a message that was
going to an individual and was obviously not intended for the list at large.

I am embarrassed about this and will try to make sure this never happens
again.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




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From: zaluzec-at-microscopy.com
Date: Tue, 26 Jul 2005 22:02:16 -0500
Subject: [Microscopy] Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's OK Chuck, Scotch can do this to you! ;-)
Markus
----- Original Message -----
X-from: {cgarber-at-2spi.com}
To: {micro-at-superlink.net}
Sent: Tuesday, July 26, 2005 10:51 PM

Sergy

I don't care who you are or where your from, but I insist the rules which have
been established for over a decade are followed by all. Since you
believe you are above the rules so be it. You are welcome to
go elsewhere.

I have canceled your subscription to the Listserver.


Nestor
Microscopy SysOp



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From: tina-at-pbrc.hawaii.edu
Date: Tue, 26 Jul 2005 23:31:26 -0500
Subject: [Microscopy] Daily newsletter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Greg-

Are you thinking of having a Saturday newsletter? There was one in
Savannah... I don't critical.

My cell phone number is 808-428-7546

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


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From: tina-at-pbrc.hawaii.edu
Date: Tue, 26 Jul 2005 23:37:38 -0500
Subject: [Microscopy] Sorry for mass post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yikes! I laugh when people inadvertantly post to the entire List, and now
I've done it! Sorry! And missing a couple of words, too. I'm tired...

See many of you in Honolulu next week!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



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From: schooley-at-mcn.org
Date: Tue, 26 Jul 2005 23:47:57 -0500
Subject: [Microscopy] Re: Sorry for mass post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Yikes! I laugh when people inadvertantly post to the entire List, and now
} I've done it! Sorry! And missing a couple of words, too. I'm tired...

And now everyone has your cell #...

CS
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

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From: as-at-astonmet.com
Date: Wed, 27 Jul 2005 07:41:31 -0500
Subject: [Microscopy] Re: Apology to the list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chuck,

No apology needed. There are laws and ethics that we overtly or tacitly
agree to abide by. If there is language in a contract, regulation or law
then we would all like to believe that our peers actually abide by those
terms.

We all have financial issues to contend with and I would hope our community
conducts it business/research/education with respect and fairness to
others. If an NSF grant or monies require directing a commercial service
AFM inquiry to a service lab in Indiana, then it should be done without
question. It is a moral and legal obligation. No excuses just as that
Indiana lab is not excused from paying taxes which in turn supports those
grants and institutions.

Alan Stone


At 09:52 PM 7/26/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com


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From: phillipst-at-missouri.edu
Date: Wed, 27 Jul 2005 08:14:54 -0500
Subject: [Microscopy] Re: Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor: I would respectfully ask you to reconsider dropping Sergey from the
list. His comment was not that egrious. He is a very active member of this
listserver and it would be a shame to drop him. I have never met him and
this comment is not motivated by friendship or a personal tie. Tom Phillips

At 10:03 PM 07/26/05, you wrote:



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Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: eschumacher-at-mccrone.com
Date: Wed, 27 Jul 2005 08:17:39 -0500
Subject: [Microscopy] Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor,

I was frankly relieved to see you step in a couple of times on this one,
and your reminder to everyone to review the rules is obviously
necessary. (I had already pulled them out myself yesterday, as a check
against some of what was being posted.)

I was surprised by the tone and the subjectivity of some of the initial
responses to what was obviously an amusing posting, and people do need
to be reminded that the listserver is an educational/informational
exchange, rather than a personal soapbox. I don't think it's an
accident that the words, 'not a right, but a privilege', show up more
than once in the rules.

Keep up the good work!

See you next week,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************

-----Original Message-----
X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
Sent: Tuesday, July 26, 2005 10:03 PM
To: Elaine F. Schumacher

Sergy

I don't care who you are or where your from, but I insist the rules
which have
been established for over a decade are followed by all. Since you
believe you are above the rules so be it. You are welcome to
go elsewhere.

I have canceled your subscription to the Listserver.


Nestor
Microscopy SysOp



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From: zaluzec-at-microscopy.com
Date: Wed, 27 Jul 2005 08:35:03 -0500
Subject: [Microscopy] Administrivia: Posting to the List vs Replying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

I've gotten several questions lately on why things are operating slighlty differently recently.
Particuliarly with respect to replies. If your interested please read on otherwise
just trash this.

As you know SPAM has grown enormously this list gets "attacked" daily. The
rate is approaching 1000 spam messages per day. Most of these were stopped but
about one or two every week managed to get through.

What you MAY NOT know is that every message that is rejected by the listserver
software gets a reply sent to the originator, letting them know there was a problem
and how to deal with it. A number of you occassional get this message when there
a problem with things you try to post. However on average this is a low number.

What you DEFINITELY don't know is that alot of the SPAM addresses are bogus and various
Email servers just return the "There is a problem with your Email" message as undeliverable.
Now think for a minute these messages are returned to someone and guess who that is....

Yep I've get barraged by huge numbers of not only spam but also literally hundreds of
rejected mail messages the number of which grows daily. Being somewhat consciencous
I check each just in case it was a real subscriber that had a problem.

The long and short of it was that things were just getting unmanagable,
and I had to redesign the filter software. This has reduced the probem from 750+ junk
messages/day to 1 or 2. The unfortunate thing is that now every message
has a REPLY line which say microscopy listserver
and it has a FROM line indicating the originator of the Email.


The net result of this is if you REPLY the message goes to the list. If you want
to send a private message you will need to copy and paste the Email address
of the sender into a new Email message. At the moment that is the correct
procedure.

There are pro's and con's of this, the advantage is that it has made my life
alot less annoying when I get home in the evening and only have to deal with
a few problems. The Con, is the occassional, inadvertently posted message.

I'll pontificate abit on this abit longer and try to figure out a
solution to the problem, which is compatible with both my needs to occassionally
have a real life out side of this electronic world, and the convenience of
replying . To be honest, I have lots to do right now and this is not a simple
issue as there are lots of hidden loop holes which need to be managed.

For the moment just try to pay close attention. Whenever you hit reply
look at the "TO" address , the location where a message is going is displayed
on you Email and is never hidden by any good Email client program.


Nestor
Your Tired Friendly (?) Neighborhood SysOp

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14, 11 -- Date: Wed, 27 Jul 2005 08:35:02 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
14, 11 -- Subject: Administrivia: Posting to the List vs Replying
14, 11 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Wed, 27 Jul 2005 08:43:16 -0500
Subject: [Microscopy] Re: Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you, Nestor. I really hope this thread will die now.

Geoff

zaluzec-at-microscopy.com wrote:

}
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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
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8, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
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==============================End of - Headers==============================




From: Jane.LaGoy-at-bodycote.com
Date: Wed, 27 Jul 2005 08:47:35 -0500
Subject: [Microscopy] my $0.02 worth on disparagement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was enjoying the IQ discourse when it was light-hearted, but I don't
believe the Microscopy Listserver, or any other professional listserver,
should disparage people personally. It is not a question of censorship but
of human decency. What is it our parents said? "If you can't say something
nice about someone then don't say anything at all". The IQ lady publicly
wrote her opinion, so that leaves those words as open game for criticism. I
think it is unfortunate that Sergey was not allowed to make his own choice
about leaving the list; in that way, he was censored. This is my personal
opinion (and since I submitted it, you folks are all welcome to criticize
it.)

Jane L. LaGoy
R&D Engineer
Bodycote HIP
155 River Street
Andover, MA 01810


==============================Original Headers==============================
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3, 15 -- To: "Microscopy Listserver (E-mail)" {Microscopy-at-MSA.Microscopy.com}
3, 15 -- Subject: my $0.02 worth on disparagement
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From: marc.pypaert-at-yale.edu
Date: Wed, 27 Jul 2005 09:36:28 -0500
Subject: [Microscopy] Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would back this opinion. Especially since the line:
"Presumably a person of hi IQ has the ability to distinguish between
these two"
in Nestor's email could have been misinterpreted by Sergey as an
unwarranted
and disparaging remark on his IQ level. At least, this is the way I
understood
it, and apparently Sergey too. I find this very disturbing...

Marc


On Wednesday, July 27, 2005, at 09:15 AM, phillipst-at-missouri.edu wrote:

}
}
}
} -----------------------------------------------------------------------
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}
} Nestor: I would respectfully ask you to reconsider dropping Sergey
} from the
} list. His comment was not that egrious. He is a very active member of
} this
} listserver and it would be a shame to drop him. I have never met him
} and
} this comment is not motivated by friendship or a personal tie. Tom
} Phillips
}
} At 10:03 PM 07/26/05, you wrote:
}
}
}
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
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} } http://www.microscopy.com/MicroscopyListserver
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} }
} } Sergy
} }
} } I don't care who you are or where your from, but I insist the rules
} } which have
} } been established for over a decade are followed by all. Since you
} } believe you are above the rules so be it. You are welcome to
} } go elsewhere.
} }
} } I have canceled your subscription to the Listserver.
} }
} }
} } Nestor
} } Microscopy SysOp
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 7, 11 -- From zaluzec-at-microscopy.com Tue Jul 26 22:02:16 2005
} } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
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} } j6R32FsY019078
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} } 22:02:16
} } -0500
} } 7, 11 -- Mime-Version: 1.0
} } 7, 11 -- Message-Id: {p0611040cbf0ca908c2d4-at-[206.69.208.22]}
} } 7, 11 -- Date: Tue, 26 Jul 2005 22:02:14 -0500
} } 7, 11 -- To: microscopy-at-microscopy.com
} } 7, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} } 7, 11 -- Subject: Administrivia: Rules
} } 7, 11 -- Content-Type: text/plain; charset="us-ascii"
} } ==============================End of -
} } Headers==============================
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 2 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}
} ==============================Original
} Headers==============================
} 9, 20 -- From PhillipsT-at-missouri.edu Wed Jul 27 08:14:54 2005
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} -0500
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} 9, 20 -- Date: Wed, 27 Jul 2005 08:16:52 -0500
} 9, 20 -- To: microscopy-at-microscopy.com
} 9, 20 -- From: Tom Phillips {phillipst-at-missouri.edu}
} 9, 20 -- Subject: Re: [Microscopy] Administrivia: Rules
} 9, 20 -- In-Reply-To: {200507270303.j6R3335J020764-at-ns.microscopy.com}
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} FILETIME=[2CB4EFA0:01C592AD]
} ==============================End of -
} Headers==============================
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
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7, 18 -- Date: Wed, 27 Jul 2005 10:31:34 -0400
7, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
7, 18 -- Subject: Re: [Microscopy] Re: Administrivia: Rules
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From: bmollon-at-pacbell.net
Date: Wed, 27 Jul 2005 09:41:38 -0500
Subject: [Microscopy] rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor..........since you just used a derogatory and
demeaning statement about someone publically using the
listserver........are you going to remove yourself
from the list? I think the "I dont care who you are"
could have been left off and your point still made.

Nestor wrote:
"Sergy

I don't care who you are or where your from, but I
insist the rules
which have
been established for over a decade are followed by
all. Since you
believe you are above the rules so be it. You are
welcome to
go elsewhere.

I have canceled your subscription to the Listserver.


Nestor
Microscopy SysOp"



==============================Original Headers==============================
8, 14 -- From bmollon-at-pacbell.net Wed Jul 27 09:41:38 2005
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8, 14 -- Date: Wed, 27 Jul 2005 07:41:38 -0700 (PDT)
8, 14 -- From: Bill Mollon {bmollon-at-pacbell.net}
8, 14 -- Subject: rules
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==============================End of - Headers==============================




From: john.vetrano-at-pnl.gov
Date: Wed, 27 Jul 2005 10:37:10 -0500
Subject: [Microscopy] Thanks, Nestor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You do so much for this community and use such an even hand and light touch
on this list that I could not believe that he was pushing it (and not for
the first time)! You have my full support. I'd post this but I don't want
to start/continue a flood!

Cheers, John


On 7/26/05 8:03 PM, "zaluzec-at-microscopy.com" {zaluzec-at-microscopy.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Sergy
}
} I don't care who you are or where your from, but I insist the rules which have
} been established for over a decade are followed by all. Since you
} believe you are above the rules so be it. You are welcome to
} go elsewhere.
}
} I have canceled your subscription to the Listserver.
}
}
} Nestor
} Microscopy SysOp
}
}
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Tue Jul 26 22:02:16 2005
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 7, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j6R32FsY019078
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} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p0611040cbf0ca908c2d4-at-[206.69.208.22]}
} 7, 11 -- Date: Tue, 26 Jul 2005 22:02:14 -0500
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} 7, 11 -- Subject: Administrivia: Rules
} 7, 11 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================

--
********
John S. Vetrano
Sr. Research Scientist
Materials Structure and Performance Group
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0724 Fax: (509)376-6308
Email: mailto:john.vetrano-at-pnl.gov


==============================Original Headers==============================
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7, 28 -- Date: Wed, 27 Jul 2005 08:36:47 -0700
7, 28 -- From: "Vetrano, John S" {john.vetrano-at-pnl.gov}
7, 28 -- Subject: Thanks, Nestor
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==============================End of - Headers==============================




From: Winston.Wiggins-at-cshs.org
Date: Wed, 27 Jul 2005 10:46:49 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Person

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

List members et al.,
Is there any way we can "restore" the system to the point before the I.Q.
posting, thereby returning Sergey to the List? I would hate to miss his
often insightful comments. I also hate to think that any comment, understood
or misunderstood, made in the heat of passion may summarily cause expulsion
sans appeal, deservedly or not.
"Can we all just get along?!"
Winston

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, E.M. Pathology Asst.
CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
Electron Microscopy, LLSPT, A823-A828
8700 Beverly Blvd.
Los Angeles, CA 90048
310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
Winston.Wiggins-at-CSHS.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

-----Original Message-----
X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
Sent: Tuesday, July 26, 2005 5:12 PM
To: Winston.Wiggins-at-CSHS.org

Sergey

A honest discussion or even disagreement about a subject or work which is
microscopy
related (even tangentially) is not prohibited.

However, it is against the rules to intentionally disparage any person
or organization on the Listserver. This rule applies equally to
Journalists as well
as Microscopists. Please review the rules which you received upon
subscription.


http://microscopy.com/MicroscopyListserver/Rules.html

Presumably a person of hi IQ has the ability to distinguish between these
two.

Nestor
Your Friendly Neighborhood SysOp

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18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org}
18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of a Pers
18, 25 -- on
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From: tonygr-at-MIT.EDU
Date: Wed, 27 Jul 2005 11:30:48 -0500
Subject: [Microscopy] Re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I tend to agree with Winston. This list is too valuable to be torn
asunder by one topic and a few heightened emotions. Perhaps when many
of us unsubscribe while attending M&M 2005 we can "reboot".


Joseph

Joseph M. Oparowski
Center for Materials Science - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313



-----Original Message-----
X-from: Winston.Wiggins-at-cshs.org [mailto:Winston.Wiggins-at-cshs.org]
Sent: Wednesday, July 27, 2005 11:50 AM
To: Oparowski, Joseph

List members et al.,
Is there any way we can "restore" the system to the point before the
I.Q. posting, thereby returning Sergey to the List? I would hate to miss
his often insightful comments. I also hate to think that any comment,
understood or misunderstood, made in the heat of passion may summarily
cause expulsion sans appeal, deservedly or not.
"Can we all just get along?!"
Winston

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, E.M. Pathology Asst.
CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
Electron Microscopy, LLSPT, A823-A828
8700 Beverly Blvd.
Los Angeles, CA 90048
310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
Winston.Wiggins-at-CSHS.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

-----Original Message-----
X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
Sent: Tuesday, July 26, 2005 5:12 PM
To: Winston.Wiggins-at-CSHS.org

Sergey

A honest discussion or even disagreement about a subject or work which
is microscopy related (even tangentially) is not prohibited.

However, it is against the rules to intentionally disparage any person
or organization on the Listserver. This rule applies equally to
Journalists as well as Microscopists. Please review the rules which
you received upon
subscription.


http://microscopy.com/MicroscopyListserver/Rules.html

Presumably a person of hi IQ has the ability to distinguish between
these two.

Nestor
Your Friendly Neighborhood SysOp

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18, 25 -- From winston.wiggins-at-cshs.org Wed Jul 27 10:46:49 2005 18, 25
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18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} 18, 25 --
To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 18, 25 --

I have to say I was waiting for Chuck's response on this thread - my jaw
dropped when I read it, until the explanation came in the next e-mail!!!

More seriously--

Most of the discussion on this thread has been about the harm done to
commercial labs by university labs "undercutting", "poaching customers" -
or whatever description you want to apply, and by the need to determine the
"true" cost of providing the service. I think, though, that there is a
serious problem for the university labs themselves in allowing commercial
service activities to become an important part of their operation.

Any operation has a core mission. The proprietor of any successful
business knows the importance of identifying and maintaining their core
mission, and the ex-proprietors of many failed businesses know the costs of
straying from the mission. While individual organizations vary, the core
mission of a university characterization laboratory is not the same as that
of a commercial laboratory. In a university our basic mission is education
through the generation of research results; that of the company is to
generate a profit through providing an analytical service. These
differences, though in some areas subtle, lead to totally different
approaches to the way we interact with our clients, the way we manage our
instrumentation, our time, etc, etc.

The government (through the NSF, in my case) contributes towards the cost
of setting up and maintaining my characterization facilities. If we
generate first-class results on new and interesting materials, then this is
considered money well-spent. If I dilute this by taking on commercial
work, even if this leads to a reduction in the amount of "subsidy", I am
reducing the rationale for getting any support, and in general diluting the
reputation of my laboratories.

I realize that not everyone in an academic environment has my good fortune
to work for an administration that supports the ideas I have just
expressed, and I also stress again that we each work in unique
environments, but in general I would suggest that in most cases commercial
clients are best left to commercial laboratories. A commercial client is
generally looking for an analytical service, rather than to be educated.

Tony Garratt-Reed.


At 10:46 PM 7/26/2005, you wrote:



} ----------------------------------------------------------------------------
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***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


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From: uti-at-direcpc.com
Date: Wed, 27 Jul 2005 11:32:22 -0500
Subject: [Microscopy] Re: Discussion of high IQ vs Disparagement of a Pers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree, we need to re-boot!
It is great place and Sergey's knowledge is needed for us.
Thanks,
Vlad


At 11:24 AM 7/27/2005 -0500, you wrote:



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From: frank.karl-at-degussa.com
Date: Wed, 27 Jul 2005 11:46:03 -0500
Subject: [Microscopy] golden rule applies here

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Folks, lets remenber the Golden Rule with our dealing with the list
server....

The man with the gold get to make the rules. Simple is it not?

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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==============================Original Headers==============================
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From: mochs-at-gwdg.de
Date: Wed, 27 Jul 2005 11:54:31 -0500
Subject: [Microscopy] Course announcement: Workshop on recent stereology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

Anybody wishing to integrate quantitative analysis by design-based
stereology into his/her studies might find this interesting. Please don´t
hesitate to contact me for further details.

Best regards,
Matthias



WORKSHOP ON RECENT STEREOLOGY - 19-23 September 2005,
Institute of Anatomy, University of Bern, Switzerland.

The workshop is intended for students and researchers from the field of
biology, medicine, and material sciences, interested in the theory and
practice of geometric sampling and measurement of 3D structures at the LM
and EM level.

The approach of the workshop is problem-based; ideally the participants
will bring questions and unsolved case studies, and the teachers will try
to supply concrete and detailed solutions, eventually explaining the
underlying theory in short ad-hoc lectures. No strong background in
mathematics or statistics is assumed. Central concepts of geometric
sampling and estimation will be introduced at the beginning. Sampling
protocols are also demonstrated on real tissue in several Laboratory sessions.

Instructors: L.M. Cruz-Orive, M. Geiser, M. Ochs.
Details and registration: http://www.ana.unibe.ch/event/ster/index.html





Matthias Ochs, M.D.
Institute of Anatomy
Experimental Morphology Unit
University of Bern
Baltzerstr. 2
CH-3000 Bern 9
Switzerland
Phone: +41 31 631 4624
Fax: +41 31 631 3807
E-mail: ochs-at-ana.unibe.ch

==============================Original Headers==============================
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From: mccaulak-at-wfu.edu
Date: Wed, 27 Jul 2005 12:11:28 -0500
Subject: [Microscopy] Re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, now that hopefully everyone is finished beating me down and
questioning my ethics, I'd like to offer a bit more of an explanation into
the reason for my posted question.

First of all, my facility exists to serve my departments needs and those
needs alone. The "outsider" in question is a former student who works at
another non-profit and who is establishing a pedagogical relationship with
us which will include teaching, mentoring, and graduate student research.
This person simply asked about providing monetary compensation if the need
arose. I was at a loss for how to answer and so thought I would ask the
listserv about this.

This is not a situation in which private labs are being undercut or a
situation in which an academic lab is pursing commercial sources of funding.

Thanks to Debby for her instructive post on an appropriate methodology for
arriving at a cost structure.

AM



-----Original Message-----
X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU]
Sent: Wednesday, July 27, 2005 12:34 PM
To: mccaulak-at-wfu.edu

I have to say I was waiting for Chuck's response on this thread - my jaw
dropped when I read it, until the explanation came in the next e-mail!!!

More seriously--

Most of the discussion on this thread has been about the harm done to
commercial labs by university labs "undercutting", "poaching customers" -
or whatever description you want to apply, and by the need to determine the
"true" cost of providing the service. I think, though, that there is a
serious problem for the university labs themselves in allowing commercial
service activities to become an important part of their operation.

Any operation has a core mission. The proprietor of any successful
business knows the importance of identifying and maintaining their core
mission, and the ex-proprietors of many failed businesses know the costs of
straying from the mission. While individual organizations vary, the core
mission of a university characterization laboratory is not the same as that
of a commercial laboratory. In a university our basic mission is education
through the generation of research results; that of the company is to
generate a profit through providing an analytical service. These
differences, though in some areas subtle, lead to totally different
approaches to the way we interact with our clients, the way we manage our
instrumentation, our time, etc, etc.

The government (through the NSF, in my case) contributes towards the cost
of setting up and maintaining my characterization facilities. If we
generate first-class results on new and interesting materials, then this is
considered money well-spent. If I dilute this by taking on commercial
work, even if this leads to a reduction in the amount of "subsidy", I am
reducing the rationale for getting any support, and in general diluting the
reputation of my laboratories.

I realize that not everyone in an academic environment has my good fortune
to work for an administration that supports the ideas I have just
expressed, and I also stress again that we each work in unique
environments, but in general I would suggest that in most cases commercial
clients are best left to commercial laboratories. A commercial client is
generally looking for an analytical service, rather than to be educated.

Tony Garratt-Reed.


At 10:46 PM 7/26/2005, you wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


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From: jmkrupp-at-cats.ucsc.edu
Date: Wed, 27 Jul 2005 12:23:07 -0500
Subject: [Microscopy] stain solutions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

I have a small lab, we do mostly basic EM type work.

Now a days, we don't do much sectioning for the TEM. Once and a while
someone wants me to do some sectioning, but many weeks (months) may pass
between requests.

I am trying to figure out a way to manage the post staining solutions so I
don't have to mix them up every time and/or how to make up just a little
for the occasional job.

Any neat tricks or systems for storing post stains like lead citrate and
uranyl acetate out there?

How about storage life time and/or ideas about mixing minimum quantities.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: gwe-at-ufl.edu
Date: Wed, 27 Jul 2005 12:25:46 -0500
Subject: [Microscopy] user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
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One issue on this topic is that those of us at state institutions
have an obligation to serve the taxpayers of our state as well as the
scientists on our respective campuses. Agricultural extension services
are an example of organized fulfillment of such obligations. Many of
their services are absolutely free. When external customers come to us
they are more often looking for help with the science behind their
projects rather than merely an analytical service. When their tax
dollars support our salaries and other costs, they can certainly expect
a break. These relationships could be interpreted as
contracts-for-research, which are perfectly legitimate arrangements.

Greg Erdos

tonygr-at-MIT.EDU wrote:

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From: redhair-at-stanford.edu
Date: Wed, 27 Jul 2005 12:47:43 -0500
Subject: [Microscopy] Re: stain solutions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jon. One of the best tricks I have found over the years is to weigh out
small amounts of lead citrate (0.1 to 0.4 grams) into 15 ml centrifuge
tubes. When you need to make stain, add 1ml of carbonate free 1N NaOH to
the tube to dissolve the lead(solution should be clear). Then add 9 mls of
dd water and shake well. Put solution through a syringe filter and it is
ready for use. I stain 30 seconds to 1 minute. Never have a problem with
precipitate and stain is fresh every time. JoAnn
At 12:27 PM 7/27/2005 -0500, you wrote:



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Department of Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856


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From: gcc-at-couger.com
Date: Wed, 27 Jul 2005 12:49:04 -0500
Subject: [Microscopy] Re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Your situation is more like extension at a land grant school
than work for hire.

And there are many ways to view it. If it is an ongoing thing
estimated the cost of materials and labor he will use what you
think it right then decide how high up the ladder you go to get
a decision.

If you use any services in a similar way use them for a pattern.

For things that cost small amounts of money time it often cost
less to give them away than to go the trouble of book keeping
and collecting them if no method is in place to do it.

I have see it done were the party need help bought supplies for
the department that helped him.

None of these are in line with accounting guide lines but use
good sense as their basis. I don't know how much trouble that
can get you in these days.

Gordon
Gordon Couger
Stillwater, OK
www.couger.com/gcouger

mccaulak-at-wfu.edu wrote:
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} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Well, now that hopefully everyone is finished beating me down and
} questioning my ethics, I'd like to offer a bit more of an explanation into
} the reason for my posted question.
}
} First of all, my facility exists to serve my departments needs and those
} needs alone. The "outsider" in question is a former student who works at
} another non-profit and who is establishing a pedagogical relationship with
} us which will include teaching, mentoring, and graduate student research.
} This person simply asked about providing monetary compensation if the need
} arose. I was at a loss for how to answer and so thought I would ask the
} listserv about this.
}
} This is not a situation in which private labs are being undercut or a
} situation in which an academic lab is pursing commercial sources of funding.
}
} Thanks to Debby for her instructive post on an appropriate methodology for
} arriving at a cost structure.
}
} AM
}
}
}
} -----Original Message-----
} X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU]
} Sent: Wednesday, July 27, 2005 12:34 PM
} To: mccaulak-at-wfu.edu
} Subject: [Microscopy] Re: user charges for SEM and related
}
}
}
}
} ----------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------------
}
} I have to say I was waiting for Chuck's response on this thread - my jaw
} dropped when I read it, until the explanation came in the next e-mail!!!
}
} More seriously--
}
} Most of the discussion on this thread has been about the harm done to
} commercial labs by university labs "undercutting", "poaching customers" -
} or whatever description you want to apply, and by the need to determine the
} "true" cost of providing the service. I think, though, that there is a
} serious problem for the university labs themselves in allowing commercial
} service activities to become an important part of their operation.
}
} Any operation has a core mission. The proprietor of any successful
} business knows the importance of identifying and maintaining their core
} mission, and the ex-proprietors of many failed businesses know the costs of
} straying from the mission. While individual organizations vary, the core
} mission of a university characterization laboratory is not the same as that
} of a commercial laboratory. In a university our basic mission is education
} through the generation of research results; that of the company is to
} generate a profit through providing an analytical service. These
} differences, though in some areas subtle, lead to totally different
} approaches to the way we interact with our clients, the way we manage our
} instrumentation, our time, etc, etc.
}
} The government (through the NSF, in my case) contributes towards the cost
} of setting up and maintaining my characterization facilities. If we
} generate first-class results on new and interesting materials, then this is
} considered money well-spent. If I dilute this by taking on commercial
} work, even if this leads to a reduction in the amount of "subsidy", I am
} reducing the rationale for getting any support, and in general diluting the
} reputation of my laboratories.
}
} I realize that not everyone in an academic environment has my good fortune
} to work for an administration that supports the ideas I have just
} expressed, and I also stress again that we each work in unique
} environments, but in general I would suggest that in most cases commercial
} clients are best left to commercial laboratories. A commercial client is
} generally looking for an analytical service, rather than to be educated.
}
} Tony Garratt-Reed.
}
}
} At 10:46 PM 7/26/2005, you wrote:
}
}
}
}
} } ---------------------------------------------------------------------------
}
} -
}
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: lcgould-at-med.cornell.edu
Date: Wed, 27 Jul 2005 13:17:08 -0500
Subject: [Microscopy] Re: stain solutions

Contents Retrieved from Microscopy Listserver Archives
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Jon,
I make up my lead citrate using the Venable & Coggeshall method
(0.01% Pb citrate in 10 ml of water + 1 drop 10N NaOH) and store it
in a syringe that is wrapped in foil. If I keep the syringe wrapped
and its tip capped, the stain is quite stable for a long time. I use
a 0.2 micron filter on the syringe to dispense the stain.
I usually do en bloc staining with Ur Ac in water just before I begin
my dehydration steps, and so I can usually avoid staining the
sections with more Ur Ac. That solution too is pretty stable if kept
in a foil-wrapped bottle. I've been using a 1.5% solution, but there
was a recent thread on that topic where the concensus was that newer
bottles of "deplete" Ur Ac necessitated higher concentrations (up to
8%, if I remember correctly).
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: tina-at-pbrc.hawaii.edu
Date: Wed, 27 Jul 2005 13:24:55 -0500
Subject: [Microscopy] Re: stain solutions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Jonathan-

I make up 10 ml of each of my stains and store them in syringes fitted
with 0.2 micron filters, excluding air. They keep pretty well this way -
weeks to months at a time. I wrap the uranyl acetate syringe with aluminum
foil. I inspect for obvious precipitates before use. Expel several drops
through the filter before use.

Are you coming to M&M in Honolulu?

Aloha,
Tina


} I have a small lab, we do mostly basic EM type work.
}
} Now a days, we don't do much sectioning for the TEM. Once and a while
} someone wants me to do some sectioning, but many weeks (months) may pass
} between requests.
}
} I am trying to figure out a way to manage the post staining solutions so I
} don't have to mix them up every time and/or how to make up just a little
} for the occasional job.
}
} Any neat tricks or systems for storing post stains like lead citrate and
} uranyl acetate out there?
}
} How about storage life time and/or ideas about mixing minimum quantities.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


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From: jquinn-at-www.matscieng.sunysb.edu
Date: Wed, 27 Jul 2005 13:25:14 -0500
Subject: [Microscopy] re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
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Folks

I have to slightly disagree with the long time
debate about academic vs commercial labs.

My scope was partially purchased with non-NSF funds.
Those funds were to encourage non-academic use.

Additionally, we have an administration that strongly
encourages us to help local industry with University
resources.

Infact, we have commercial consultants who use our equipment.

Hence, that is not competition.

regards,

JQuinn



**********************************************************
Dr. Jim Quinn james.quinn-at-stonybrook.edu
Materials Science 631-632-6663 FAX:8052
Stony Brook University www.matscieng.stonybrook.edu
Stony Brook, New York 11794 - 2275
**********************************************************


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From: bozzola-at-siu.edu
Date: Wed, 27 Jul 2005 13:25:28 -0500
Subject: [Microscopy] Re: Apology to the list

Contents Retrieved from Microscopy Listserver Archives
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Chuck,

I'm sure everyone laughed out loud at this posting.

Thanks for brightening up our day!!!

John


}
} Please accept my apologies for inadvertently sending out a message that was
} going to an individual and was obviously not intended for the list at large.
}
} I am embarrassed about this and will try to make sure this never happens
} again.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President
} SPI SUPPLIES FAX: 1-610-436-5755
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--
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John J. Bozzola, Ph.D., Director
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750 Communications Drive - MC 4402
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From: Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Wed, 27 Jul 2005 13:28:42 -0500
Subject: [Microscopy] cameras in labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

We are currently considering adding security cameras in each of our EM
labs.

We have a very large number of users, both during working hours and
after-hours in SEM and TEM rooms. Just recently, we have had a few
incidents where people have damaged the instrument and did not come
forward to admit it.

Although we can see who logged on to the computers, you can still damage
the instrument without logging in. We believe that a CCTV in each of the
labs would be the best way to solve this problem, but unfortunately we
are faced with "privacy issues". People do not want to be watched.

Please tell me if your labs use cameras (live or log) or if you found
other ways to get around the problem.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632


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From: walck-at-southbaytech.com
Date: Wed, 27 Jul 2005 13:49:54 -0500
Subject: [Microscopy] picoammeter availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is there anyone out in microscopy land that might be willing to let me
borrow a Keithley picoammeter. I wold like to do some experiments with
an ion gun. The unit that came with the Gatan analytical stage is what
I would like to get my hands on for a few days. I believe that the
model number is a 480. I am not adverse to going to my new boss with
some spirited horse trading if you can help me out, especially if you
might be willing to part with it permanently. If someone has an even
older model that they might want to part with like a 601 or 610, that
would be useful to me also.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


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From: zaluzec-at-microscopy.com
Date: Wed, 27 Jul 2005 14:00:03 -0500
Subject: [Microscopy] Re: cameras in labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Daniel

Have a look at the TelePresence Microscopy Site

http://tpm.amc.anl.gov

Use either Netscape or IE browsers , Safari access is currently broken I'll fix that
after the MM2005 meeting.

Nestor
Your Friendly Neighborhood SysOp



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==============================Original Headers==============================
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8, 14 -- To: microscopy-at-microscopy.com
8, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
8, 14 -- Subject: Re: [Microscopy] cameras in labs
8, 14 -- Cc: Daniel.Salamon-at-nrc-cnrc.gc.ca
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From: lcgould-at-med.cornell.edu
Date: Wed, 27 Jul 2005 15:06:30 -0500
Subject: [Microscopy] Re: rules

Contents Retrieved from Microscopy Listserver Archives
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Dear bmollon


How on earth do you read Nestor's email as "derogatory and demeaning"?

Do you see some equivalence between saying "I don't care who you are" and
describing someone else as practising "crap journalism"?

What color is the sky in your world?

And how about having the courage to identify yourself?

Anonymous postings don't rate very highly with most people.

cheers

rtch




Date sent: Wed, 27 Jul 2005 09:42:24 -0500
To: r.sims-at-auckland.ac.nz
X-from: bmollon-at-pacbell.net
Send reply to: microscopy-at-microscopy.com

Hey guys,
I thing some of this is coming down to the issue of reading
tone/intent into words in print. When Nestor wrote "I don't care who
you are" , MY interpretation was "no one has special rights or
privileges" not "I don't give a damn about you". (Just my humble
reading).
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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From: K.venner-at-ion.ucl.ac.uk
Date: Wed, 27 Jul 2005 15:11:44 -0500
Subject: [Microscopy] viaWWW: tem flat embedding moulds

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Wednesday, July 27, 2005 at 10:27:20
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: Kerrie

Organization: ION, UCL, UK

Title-Subject: [Filtered] tem flat embedding moulds

Question: I have some well used, much coveted flat embedding moulds for TEM samples. They are shallow, hexagonal-shaped recesses with an arrowhead on one side, and are in a pale blue rubbery material. Unfortunately they are beginning to perish, and we have no idea where we bought them from originally. We are desperate to replace them. Any ideas or suppliers out there?

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: deerinck-at-ncmir.ucsd.edu
Date: Wed, 27 Jul 2005 15:13:26 -0500
Subject: [Microscopy] viaWWW: Staff Positions Available at the National Center for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (deerinck-at-ncmir.ucsd.edu) from http://microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, July 27, 2005 at 12:48:11
---------------------------------------------------------------------------

Email: deerinck-at-ncmir.ucsd.edu
Name: Tom Deerinck

Organization: NCMIR, UCSD

Title-Subject: [Filtered] MListserver:

Question: Staff Positions Available at the National Center for Microscopy and Imaging Research at the University of California, San Diego, CA

Two staff positions in TEM and 3D tomographic reconstruction are available immediately. The successful candidate should have a BS or MS in relevant fields of biological sciences and have experience in either 1) transmission electron microscopy or 2) image processing and/or three-dimensional reconstruction techniques.

Qualifications for the first position (Transmission Electron Microscopist) are:
_ Experienced TEM operation (operation, alignment, image recording)
_ Proficiency in sectioning of plastic embedded biological specimens (microtomy)
_ Familiarity in preparation of biological material for plastic embedding (fixation, dehydration and embedding techniques)
_ Experience in bacteriology, immunocytochemistry, cell biology or fluorescence/confocal microscopy preferred but not essential.

Qualifications for the second position (Image Processing Scientist) are:
_ Experience in image processing and evaluating EM images
_ Experience in three-dimensional tomographic reconstruction
_ Familiarity using Linux or Unix, Mac and PC operating systems
_ Proficiency in computer visualization and/or animation package
_ General biology background with a preferred emphasis on bacterial systems.

Excellent written and verbal skills are expected. The candidates will be working in a large interdisciplinary research center located in the University of California San Diego main campus and will have access to state of the art electron microscopes. For more information on our laboratory, please visit http://www.ncmir.ucsd.edu.
Please forward this message to all appropriate personnel. Applicants should send curriculum vitae, bibliography, a brief description of present research activities and plans and the names and contact information of three references to:

Dr. Mark Ellisman or Mr. Thomas Deerinck
University of California at San Diego
1000 Basic Science Building MC 0608
9500 Gilman Drive
La Jolla, CA 92093-0608
858-534-4583 (phone) mark-at-ncmir.ucsd.edu (email)
858-534-7497 (fax) deerinck-at-ncmir.ucsd.edu

We will also be available at the MSA meeting next week in Hawaii.


---------------------------------------------------------------------------

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13, 13 -- From: deerinck-at-ncmir.ucsd.edu (by way of MicroscopyListserver)
13, 13 -- Subject: viaWWW: Staff Positions Available at the National Center for
13, 13 -- Microscopy and Imaging Research UCSD
13, 13 -- Content-Type: text/plain; charset="us-ascii"
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From: lcgould-at-med.cornell.edu
Date: Wed, 27 Jul 2005 15:25:38 -0500
Subject: [Microscopy] Re: viaWWW: tem flat embedding moulds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

They sound like Chien molds to me....I know that EMS carries them,
probably other suppliers too.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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From: leswes-at-shaw.ca
Date: Wed, 27 Jul 2005 15:57:31 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'll second this.

--
Lesley Weston


} From: Winston.Wiggins-at-cshs.org
} Reply-To: microscopy-at-microscopy.com
} Date: Wed, 27 Jul 2005 10:54:20 -0500
} To: leswes-at-shaw.ca
} Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers
}
}
}
}
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} List members et al.,
} Is there any way we can "restore" the system to the point before the I.Q.
} posting, thereby returning Sergey to the List? I would hate to miss his
} often insightful comments. I also hate to think that any comment, understood
} or misunderstood, made in the heat of passion may summarily cause expulsion
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} "Can we all just get along?!"
} Winston
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Winston W. Wiggins, E.M. Pathology Asst.
} CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
} Electron Microscopy, LLSPT, A823-A828
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} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
} -----Original Message-----
} X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
} Sent: Tuesday, July 26, 2005 5:12 PM
} To: Winston.Wiggins-at-CSHS.org
} Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Person
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Sergey
}
} A honest discussion or even disagreement about a subject or work which is
} microscopy
} related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally disparage any person
} or organization on the Listserver. This rule applies equally to
} Journalists as well
} as Microscopists. Please review the rules which you received upon
} subscription.
}
}
} http://microscopy.com/MicroscopyListserver/Rules.html
}
} Presumably a person of hi IQ has the ability to distinguish between these
} two.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
} } ---------------------------------------------------------------------------
} -
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ---------------------------------------------------------------------------
} -
} }
} } Nestor
} } I don't see any reason why we could not discuss the quality of somebody's
} } work even if it's a journalist with former (?) high IQ. Is it prohibited
} } to discuss people's work with high IQ on this ListServer? Sergey
} }
} } At 03:51 PM 7/26/2005, you wrote:
} } ---------------------------------------------------------------------------
} -
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } --------------------------------------------------------------------------
} --
} } }
} } } Beth & everyone else
} } }
} } } There was no problem with Beth's initial posting and no apology
} } } was needed here Beth. Sorry if I appeared to have come down on you.
} } }
} } } The followups IMHO were starting to drift into critiques of Marilyn
} whomever
} } } and her "crap Journalism" and I was trying to nip that direction of
} } } critique in the bud. However, I see I had the opposite effect. Oh
} well.....
} } }
} } } As Dorrance said, everyone can use a grin occassionally .
} } }
} } }
} } } Nestor
} } }
} } }
} } } --------------------------------------------------------------------------
} --
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } ---------------------------------------------------------------------------
} -
} } } }
} } } } I apologize to the list for posting Marilyn vos Savant's statement on
} } } } microscopy. I just wanted to share the article because I thought it was
} } } } humorous...I shall refrain from such postings.
} } } } best,
} } } } Beth
} } } }
} } } } **********************************************************************
} } } } Beth Richardson
} } } } EM Lab Coordinator
} } } } Plant Biology Department
} } } } University of Georgia
} } } } Athens, GA 30602-7271
} } } }
} } } } Phone - (706) 542-1790 & FAX - (706) 542-1805
} } } } http://www.plantbio.uga.edu/emlab
} } } }
} } } } "Between the two evils,
} } } } I always pick the one I never tried before". Mae West (1893-1980)
} } } } *******************************************************************
} } } }
} } } } "And it's only the giving that makes you what you are".
} } } } Wond'ring Aloud, Jethro Tull (Aqualung)
} } } }
} } } } ************************************************************************
}
}
}
}
} Important Warning: This message is intended for the use of the person or
} entity to which it is addressed and may contain information that is
} privileged and confidential, the disclosure of which is governed by
} aplicable law. If the reader is not the intended recipient, or the employee
} or agent responsible for delivering it to the intended recipient, you are
} hereby notified that any dissemination, distribution or copying of this
} information is STRICTLY PROHIBITED.
}
} If you have received this message in error, please notify us immediately,
} by calling (310) 423-6428 -- and destroy the related message. Thank You for
} your cooperation.
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}
}
} ==============================Original Headers==============================
} 18, 25 -- From winston.wiggins-at-cshs.org Wed Jul 27 10:46:49 2005
} 18, 25 -- Received: from csip1.csmc.edu (CSIP1.csmc.edu [192.231.133.35])
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} {B5AEAEDBB5D44C47BFE2A6D791329D7F2ADC7D-at-EXCHANGE24.csmc.edu}
} 18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org}
} 18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} 18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of
} a Pers
} 18, 25 -- on
} 18, 25 -- Date: Wed, 27 Jul 2005 08:46:46 -0700
} 18, 25 -- MIME-Version: 1.0
} 18, 25 -- X-Mailer: Internet Mail Service (5.5.2653.19)
} 18, 25 -- Content-Type: text/plain
} ==============================End of - Headers==============================


==============================Original Headers==============================
5, 27 -- From leswes-at-shaw.ca Wed Jul 27 15:57:28 2005
5, 27 -- Received: from pd4mo2so.prod.shaw.ca (shawidc-mo1.cg.shawcable.net [24.71.223.10])
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5, 27 -- 27 Jul 2005 14:57:26 -0600 (MDT)
5, 27 -- Date: Wed, 27 Jul 2005 13:58:42 -0700
5, 27 -- From: Lesley Weston {leswes-at-shaw.ca}
5, 27 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers
5, 27 -- In-reply-to: {200507271554.j6RFsKKs004617-at-ns.microscopy.com}
5, 27 -- To: microscopy-at-microscopy.com
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==============================End of - Headers==============================




From: DFORAN-at-ORA.FDA.GOV
Date: Wed, 27 Jul 2005 16:00:11 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Aye.

-----Original Message-----
X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca]
Sent: Wednesday, July 27, 2005 3:58 PM
To: Foran, David A

I'll second this.

--
Lesley Weston


} From: Winston.Wiggins-at-cshs.org
} Reply-To: microscopy-at-microscopy.com
} Date: Wed, 27 Jul 2005 10:54:20 -0500
} To: leswes-at-shaw.ca
} Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a
} Pers
}
}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} List members et al.,
} Is there any way we can "restore" the system to the point before the
} I.Q. posting, thereby returning Sergey to the List? I would hate to
} miss his often insightful comments. I also hate to think that any
} comment, understood or misunderstood, made in the heat of passion may
} summarily cause expulsion sans appeal, deservedly or not. "Can we all
} just get along?!" Winston
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Winston W. Wiggins, E.M. Pathology Asst.
} CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron
} Microscopy, LLSPT, A823-A828 8700 Beverly Blvd.
} Los Angeles, CA 90048
} 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
} Winston.Wiggins-at-CSHS.org
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
} -----Original Message-----
} X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
} Sent: Tuesday, July 26, 2005 5:12 PM
} To: Winston.Wiggins-at-CSHS.org
} Subject: [Microscopy] Discussion of high IQ vs Disparagement of a
} Person
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Sergey
}
} A honest discussion or even disagreement about a subject or work
} which is microscopy related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally disparage any person
} or organization on the Listserver. This rule applies equally to
} Journalists as well as Microscopists. Please review the rules which
} you received upon
} subscription.
}
}
} http://microscopy.com/MicroscopyListserver/Rules.html
}
} Presumably a person of hi IQ has the ability to distinguish between
} these two.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
} } ---------------------------------------------------------------------
} } ------
} -
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
---------------------------------------------------------------------------
} -
} }
} } Nestor
} } I don't see any reason why we could not discuss the quality of
} } somebody's work even if it's a journalist with former (?) high IQ.
} } Is it prohibited to discuss people's work with high IQ on this
} } ListServer? Sergey
} }
} } At 03:51 PM 7/26/2005, you wrote:
} } ---------------------------------------------------------------------
} } ------
} -
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } --------------------------------------------------------------------
} } } ------
} --
} } }
} } } Beth & everyone else
} } }
} } } There was no problem with Beth's initial posting and no apology
} } } was needed here Beth. Sorry if I appeared to have come down on you.
} } }
} } } The followups IMHO were starting to drift into critiques of
} } } Marilyn
} whomever
} } } and her "crap Journalism" and I was trying to nip that direction of
} } } critique in the bud. However, I see I had the opposite effect. Oh
} well.....
} } }
} } } As Dorrance said, everyone can use a grin occassionally .
} } }
} } }
} } } Nestor
} } }
} } }
} } } --------------------------------------------------------------------
} } } ------
} --
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } ---------------------------------------------------------------------
} } ------
} -
} } } }
} } } } I apologize to the list for posting Marilyn vos Savant's statement
} } } } on microscopy. I just wanted to share the article because I thought
} } } } it was humorous...I shall refrain from such postings. best,
} } } } Beth
} } } }
} } } } *******************************************************************
} } } } ***
} } } } Beth Richardson
} } } } EM Lab Coordinator
} } } } Plant Biology Department
} } } } University of Georgia
} } } } Athens, GA 30602-7271
} } } }
} } } } Phone - (706) 542-1790 & FAX - (706) 542-1805
} } } } http://www.plantbio.uga.edu/emlab
} } } }
} } } } "Between the two evils,
} } } } I always pick the one I never tried before". Mae West (1893-1980)
} } } } *******************************************************************
} } } }
} } } } "And it's only the giving that makes you what you are". Wond'ring
} } } } Aloud, Jethro Tull (Aqualung)
} } } }
} } } } *******************************************************************
} } } } *****
}
}
}
}
} Important Warning: This message is intended for the use of the person
} or entity to which it is addressed and may contain information that is
} privileged and confidential, the disclosure of which is governed by
} aplicable law. If the reader is not the intended recipient, or the
} employee or agent responsible for delivering it to the intended
} recipient, you are hereby notified that any dissemination,
} distribution or copying of this information is STRICTLY PROHIBITED.
}
} If you have received this message in error, please notify us
} immediately, by calling (310) 423-6428 -- and destroy the related
} message. Thank You for your cooperation.
}
}
}
} ==============================Original
} Headers==============================
} 18, 25 -- From winston.wiggins-at-cshs.org Wed Jul 27 10:46:49 2005
} 18, 25 -- Received: from csip1.csmc.edu (CSIP1.csmc.edu [192.231.133.35])
} 18, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
j6RFkmjF031434
} 18, 25 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2005 10:46:48
-0500
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-0500
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[166.124.43.199])
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-0500
} (CDT)
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(5.5.2653.19)
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} 18, 25 -- Message-ID:
} {B5AEAEDBB5D44C47BFE2A6D791329D7F2ADC7D-at-EXCHANGE24.csmc.edu}
} 18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org}
} 18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} 18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement
of
} a Pers
} 18, 25 -- on
} 18, 25 -- Date: Wed, 27 Jul 2005 08:46:46 -0700
} 18, 25 -- MIME-Version: 1.0
} 18, 25 -- X-Mailer: Internet Mail Service (5.5.2653.19)
} 18, 25 -- Content-Type: text/plain
} ==============================End of -
Headers==============================


==============================Original Headers==============================
5, 27 -- From leswes-at-shaw.ca Wed Jul 27 15:57:28 2005
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[24.71.223.10])
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-0500
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for microscopy-at-microscopy.com; Wed, 5, 27 -- 27 Jul 2005 14:57:26 -0600
(MDT) 5, 27 -- Date: Wed, 27 Jul 2005 13:58:42 -0700 5, 27 -- From: Lesley
Weston {leswes-at-shaw.ca}
5, 27 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs
Disparagement of a Pers
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==============================Original Headers==============================
12, 17 -- From DFORAN-at-ORA.FDA.GOV Wed Jul 27 16:00:11 2005
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12, 17 -- From: "Foran, David A" {DFORAN-at-ORA.FDA.GOV}
12, 17 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
12, 17 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of a Per
12, 17 -- s
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From: tiekotte-at-up.edu
Date: Wed, 27 Jul 2005 16:02:23 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

aye


On 7/27/05 2:00 PM, "DFORAN-at-ORA.FDA.GOV" {DFORAN-at-ORA.FDA.GOV} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Aye.
}
} -----Original Message-----
} X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca]
} Sent: Wednesday, July 27, 2005 3:58 PM
} To: Foran, David A
} Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers
}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
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}
} I'll second this.


==============================Original Headers==============================
5, 18 -- From tiekotte-at-up.edu Wed Jul 27 16:02:23 2005
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5, 18 -- User-Agent: Microsoft-Entourage/11.0.0.040405
5, 18 -- Date: Wed, 27 Jul 2005 14:02:21 -0700
5, 18 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs Disparagement of a
5, 18 -- Per
5, 18 -- From: Ken Tiekotter {tiekotte-at-up.edu}
5, 18 -- To: {microscopy-at-microscopy.com}
5, 18 -- Message-ID: {BF0D446D.239B%tiekotte-at-up.edu}
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From: Tom.Januszewski-at-UTSouthwestern.edu
Date: Wed, 27 Jul 2005 16:14:16 -0500
Subject: [Microscopy] Re: viaWWW: tem flat embedding moulds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

They are Chien embedding molds. We got ours from Ted Pella.
Tom

Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
Dallas, TX 75390-9039
214-648-7291
FAX 214-648-6408
tom.januszewski-at-UTSouthwestern.edu


==============================Original Headers==============================
3, 17 -- From Tom.Januszewski-at-UTSouthwestern.edu Wed Jul 27 16:14:16 2005
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3, 17 -- From: "Tom Januszewski" {Tom.Januszewski-at-UTSouthwestern.edu}
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From: DusevichV-at-umkc.edu
Date: Wed, 27 Jul 2005 16:25:16 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Per

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Aye

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: DFORAN-at-ORA.FDA.GOV [mailto:DFORAN-at-ORA.FDA.GOV]
} Sent: Wednesday, July 27, 2005 4:01 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] RE: Discussion of high IQ vs
} Disparagement of a Per
}
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
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}
} Aye.
}
} -----Original Message-----
} X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca]
} Sent: Wednesday, July 27, 2005 3:58 PM
} To: Foran, David A
} Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers
}
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} --------------------------------------------------------------
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}
} I'll second this.
}
} --
} Lesley Weston
}
}
} } From: Winston.Wiggins-at-cshs.org
} } Reply-To: microscopy-at-microscopy.com
} } Date: Wed, 27 Jul 2005 10:54:20 -0500
} } To: leswes-at-shaw.ca
} } Subject: [Microscopy] RE: Discussion of high IQ vs
} Disparagement of a
} } Pers
} }
} }
} }
} }
} }
} ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} --------------
} }
} } List members et al.,
} } Is there any way we can "restore" the system to the point before the
} } I.Q. posting, thereby returning Sergey to the List? I would hate to
} } miss his often insightful comments. I also hate to think that any
} } comment, understood or misunderstood, made in the heat of
} passion may
} } summarily cause expulsion sans appeal, deservedly or not.
} "Can we all
} } just get along?!" Winston
} }
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Winston W. Wiggins, E.M. Pathology Asst.
} } CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron
} } Microscopy, LLSPT, A823-A828 8700 Beverly Blvd.
} } Los Angeles, CA 90048
} } 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
} } Winston.Wiggins-at-CSHS.org
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} }
} } -----Original Message-----
} } X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
} } Sent: Tuesday, July 26, 2005 5:12 PM
} } To: Winston.Wiggins-at-CSHS.org
} } Subject: [Microscopy] Discussion of high IQ vs Disparagement of a
} } Person
} }
} }
} ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} --------------
} }
} } Sergey
} }
} } A honest discussion or even disagreement about a subject or work
} } which is microscopy related (even tangentially) is not prohibited.
} }
} } However, it is against the rules to intentionally disparage
} any person
} } or organization on the Listserver. This rule applies equally to
} } Journalists as well as Microscopists. Please review the
} rules which
} } you received upon
} } subscription.
} }
} }
} } http://microscopy.com/MicroscopyListserver/Rules.html
} }
} } Presumably a person of hi IQ has the ability to distinguish between
} } these two.
} }
} } Nestor
} } Your Friendly Neighborhood SysOp
} }
} } }
} ---------------------------------------------------------------------
} } } ------
} } -
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} --------------------------------------------------------------
} -------------
} } -
} } }
} } } Nestor
} } } I don't see any reason why we could not discuss the quality of
} } } somebody's work even if it's a journalist with former (?)
} high IQ.
} } } Is it prohibited to discuss people's work with high IQ on this
} } } ListServer? Sergey
} } }
} } } At 03:51 PM 7/26/2005, you wrote:
} } }
} ---------------------------------------------------------------------
} } } ------
} } -
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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} } } }
} --------------------------------------------------------------------
} } } } ------
} } --
} } } }
} } } } Beth & everyone else
} } } }
} } } } There was no problem with Beth's initial posting and no apology
} } } } was needed here Beth. Sorry if I appeared to have come
} down on you.
} } } }
} } } } The followups IMHO were starting to drift into critiques of
} } } } Marilyn
} } whomever
} } } } and her "crap Journalism" and I was trying to nip that
} direction of
} } } } critique in the bud. However, I see I had the opposite effect. Oh
} } well.....
} } } }
} } } } As Dorrance said, everyone can use a grin occassionally .
} } } }
} } } }
} } } } Nestor
} } } }
} } } }
} } } }
} --------------------------------------------------------------------
} } } } ------
} } --
} } } } } The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of
} } America
} } } } } To Subscribe/Unsubscribe --
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} } } } } On-Line Help


==============================Original Headers==============================
7, 23 -- From DusevichV-at-umkc.edu Wed Jul 27 16:25:16 2005
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From: cgarber-at-2spi.com
Date: Wed, 27 Jul 2005 17:12:36 -0500
Subject: [Microscopy] University facilities being used for outside users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Tony Garratt-Reed wrote:
========================================================
I have to say I was waiting for Chuck's response on this thread - my jaw
dropped when I read it, until the explanation came in the next e-mail!!!
========================================================
Perhaps I became a bit "gun shy" after sending off a message in error. I
appreciate the understanding of those on the list for someone, like myself,
who did make an error. It has taken me more than a few hours to recover
from that.

I have operated an independent testing and analytical laboratory with its
core capabilities being built around SEM/EDS and TEM and LM since 1970. I
think I have at least some credentials to speak to the issue at hand.
However, what I have to say might be seen as "too much" for a normal
listserver posting, therefore I have posted my "reply" to URL
http://www.2spi.com/letter.html

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================







==============================Original Headers==============================
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==============================End of - Headers==============================




From: J.Nailon-at-uq.edu.au
Date: Wed, 27 Jul 2005 17:44:01 -0500
Subject: [Microscopy] Re: cameras in labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

G'day Daniel,

We used to showoff our instrumentation across the web through our
Nanoworld site, we have 14 EM columns and most were available to view
24/7. This was until we had a nasty stalking incident that left both the
client involved and our staff members unsettled until we took all
cameras off air. We now do individual presentation to schools and groups
as required and everyone is aware the cameras are ON.

Regards
JVN



Daniel.Salamon-at-nrc-cnrc.gc.ca wrote:

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--
John V Nailon
Executive Officer and Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St.Lucia QLD 4072 Australia
Phone: 617 3365 4214
Fax: 617 3365 4422
Mobile: 0423 020 680



==============================Original Headers==============================
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From: gary-at-gaugler.com
Date: Wed, 27 Jul 2005 18:19:32 -0500
Subject: [Microscopy] Trolling for SEM/STEM specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am again looking for prepared and interesting
specimens for SEM/STEM or just TEM negs. Human pathogens
are especially welcome. Standard 12mm pin stubs for
SEM are perfect. Bacteria, parasites, cancers, etc.
are good. CPD/fixed specimens coated or un-coated
are fine.

Pls contact me off-line for my payment schedule
and what you may have to sell (I keep it) or rent
(in case you want it back).

See you in HI.

gary g.


==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Wed, 27 Jul 2005 19:30:42 -0500
Subject: [Microscopy] Re: golden rule applies here

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

isn't the golden rule do uuto others before they do
unto you?
which clearly seems to be the case in a preemptive
attack

--- frank.karl-at-degussa.com wrote:

}
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} Folks, lets remenber the Golden Rule with our
} dealing with the list
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} The man with the gold get to make the rules. Simple
} is it not?
}
} Frank Karl
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__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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From: elshaw-at-MIT.EDU
Date: Wed, 27 Jul 2005 22:35:21 -0500
Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For heaven's sake, stop your anonymous bitching!

This is a scientific forum, tirelessly, patiently, and good-humoredly run for the benefit
of us all by Nestor, who certainly doesn't deserve this, not some sort of internet chat
room.

Stop sniping or quit the list.

rtch




Date sent: Wed, 27 Jul 2005 19:32:16 -0500
To: r.sims-at-auckland.ac.nz
X-from: hoffpajo-at-yahoo.com
Send reply to: microscopy-at-microscopy.com

Note to Winston et al: I see nothing in the listserver FAQ that says
a revoked subscriber can't request to be resubscribed.

I have a suspicion that much of this brouhaha can be sourced to
record heat stewing the brains of hapless millions in the Lower 48
and southern Canada.

Hawaii sounds just perfect right about now. Y'all enjoy! The rest
of us will stand in front of the refrigerator with the door open ;^)

Libby Shaw


} Date: Wed, 27 Jul 2005 10:49:35 -0500
} From: Winston.Wiggins-at-cshs.org
} Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers
}
}
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From: neuberger1234-at-comcast.net
Date: Wed, 27 Jul 2005 22:55:21 -0500
Subject: [Microscopy] Discussion of high IQ thread

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Folks,

My delete key has certainly had a workout yesterday and today. I do hope that
tomorrow brings a respite from the "heat" generated by this thread and that we
can all move on with our lives. From personal experience, life can be way too
short to expend so much energy on this issue which in the end, I believe, will
have advanced the wisdom of humankind not one iota but seems to have created ill
feelings among some folks.

Please, if you wish to reply to this message, just reply to me and not the
entire list and give this thread and the list a well deserved rest!

Damian Neuberger




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From: tanderso-at-cbs.umn.edu
Date: Thu, 28 Jul 2005 00:49:10 -0500
Subject: [Microscopy] Re: University facilities being used for outside

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The beauty of this board is the ability to disseminate the
knowledge of microscopy. This has proven useful to me as well as countless
others across the globe.

That being said, I think some of us across this board need to lighten up.

If certain things said here trigger specific emotions personally, then it's
time to bring those things up to your personal psychologist, not this
listserv.

My trigger finger is getting tired of hitting the delete key to these
emotional responses... can't we just get back to microscopy, the purpose of
this board??!

- Tracy


On 7/27/05 5:16 PM, "cgarber-at-2spi.com" {cgarber-at-2spi.com} wrote:

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} Tony Garratt-Reed wrote:
} ========================================================
} I have to say I was waiting for Chuck's response on this thread - my jaw
} dropped when I read it, until the explanation came in the next e-mail!!!
} ========================================================
} Perhaps I became a bit "gun shy" after sending off a message in error. I
} appreciate the understanding of those on the list for someone, like myself,
} who did make an error. It has taken me more than a few hours to recover
} from that.
}
} I have operated an independent testing and analytical laboratory with its
} core capabilities being built around SEM/EDS and TEM and LM since 1970. I
} think I have at least some credentials to speak to the issue at hand.
} However, what I have to say might be seen as "too much" for a normal
} listserver posting, therefore I have posted my "reply" to URL
} http://www.2spi.com/letter.html
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} Chuck
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} 7, 13 -- Subject: University facilities being used for outside users
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} 7, 13 -- X-Mailer: E-Mail Connection v3.1a
} ==============================End of - Headers==============================

-----
Tracy E. Anderson
Microscopist / Imaging Specialist
Imaging Center
University of Minnesota
Phone: 612.624.3454
Fax: 612.624.1799
http://www.cbs.umn.edu/ic/

³Science and art belong to the whole world, and before them vanish the
barriers of nationality.² - Goethe





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From: tanderso-at-cbs.umn.edu
Date: Thu, 28 Jul 2005 01:00:51 -0500
Subject: [Microscopy] Zoinks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I realize now that my previous message doesn't directly relate to the
subject line. It was mainly in response to the "IQ" post, which I thought
was interesting and humorous to read.

Bon soiree







==============================Original Headers==============================
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From: Gretchen.Ziegler-at-leica-microsystems.com
Date: Thu, 28 Jul 2005 01:01:16 -0500
Subject: [Microscopy] Gretchen Ziegler/USDER/West/Leica is out of the office.

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office starting 07/28/2005 and will not return until
08/10/2005.

I am on vacation and not able to pick up emails or voicemails. If you need
immediate help please contact customer service in Bannockburn, otherwise I
will respond on August 10th.
Thank you.
Gretchen


_____________________________________________________________________
This e-mail has been scanned for viruses by MCI's Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.mci.com

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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 07:24:11 -0500
Subject: [Microscopy] Re: golden rule applies here

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You know I just knew you couldn't resist replying the
email.
First of all there is nothing anonymous about my
email. I do believe my name appears in fron of the
email address.
2nd I think you refered to yahoo as a freebie email
account, while yes it is true I do not pay a cent for
the account, I do however pay a very high price for my
cable broadband account. How many of you on this list
server can say that when they post a message, that has
very little to do with work?
3rd you have no clue who you are talking to. I have 25
years in electron microscopy. I did however retire
from the field 2 years ago at the ripe old age of 46,
mainly to get away from people like you. I do what I
want when I want and with whomever I want. Now if you
are trying to run off those of us with experience and
something to contribute, with those you disagree you
are doing a good job.
You sir seem to know a lot more about internet chat
rooms than I do.

Finally: I leave it to Nestor to decide if your
account should be pulled for using DISPARAGING
REMARKS directed at me, quoteing you "anonymous
bitching!" I suspect he will do nothing.
Now having said all that, if you having anything
further to say to me on the subject email me directly.
I will be happy to explain myself with full vigor.
John Hoffpauir ret
637 pine street (society hill)
philadelphia pa
19106
PS does this make you happy?


--- r.sims-at-auckland.ac.nz wrote:

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} For heaven's sake, stop your anonymous bitching!
}
} This is a scientific forum, tirelessly, patiently,
} and good-humoredly run for the benefit
} of us all by Nestor, who certainly doesn't deserve
} this, not some sort of internet chat
} room.
}
} Stop sniping or quit the list.
}
} rtch
}
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} Date sent: Wed, 27 Jul 2005 19:32:16 -0500
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} } isn't the golden rule do uuto others before they
} do
} } unto you?
} } which clearly seems to be the case in a preemptive
} } attack
} }
} } --- frank.karl-at-degussa.com wrote:
} }
} } }
} } }
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} } dealing with the list } server.... } } The man
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} } you. } } } ==============================Original
} }
} } Headers============================== } 12, 17 --
} From
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} } 12, 17 --
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{OF7928F80A.6C45314D-ON8525704B.005DDFD5-8525704B.005E1722-at-degussa.com
} } } } 12, 17 -- From: frank.karl-at-degussa.com } 12,
} 17 -- Date: Wed, 27
} } Jul 2005 13:07:43 -0400 } 12, 17 -- X-MIMETrack:
} Serialize by Router
} } on } MOBUSComm01/DHexternal/US(Release
} 6.5.1|January 21, } 2004) at }
} } 12, 17 -- 07/27/2005 11:45:41 AM } 12, 17 --
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} } ==============================Original
} } Headers============================== 5, 19 --
} From hoffpajo-at-yahoo.com
} } Wed Jul 27 19:30:42 2005 5, 19 -- Received: from
} } web50201.mail.yahoo.com (web50201.mail.yahoo.com
} [206.190.38.42]) 5,
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 07:27:27 -0500
Subject: [Microscopy] Re: Discussion of high IQ thread

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


you are correct, i did however find the need to write
to this sims guy i note you have one of those
anonymous email accout he rails against.
john
--- neuberger1234-at-comcast.net wrote:

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} Folks,
}
} My delete key has certainly had a workout yesterday
} and today. I do hope that
} tomorrow brings a respite from the "heat" generated
} by this thread and that we
} can all move on with our lives. From personal
} experience, life can be way too
} short to expend so much energy on this issue which
} in the end, I believe, will
} have advanced the wisdom of humankind not one iota
} but seems to have created ill
} feelings among some folks.
}
} Please, if you wish to reply to this message, just
} reply to me and not the
} entire list and give this thread and the list a well
} deserved rest!
}
} Damian Neuberger
}
}
}
}
} ==============================Original
} Headers==============================
} 7, 16 -- From neuberger1234-at-comcast.net Wed Jul 27
} 22:55:20 2005
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 07:34:04 -0500
Subject: [Microscopy] Re: my $0.02 worth on disparagement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

there is nothing to criticize, Sergey was censored.
john from an anaoymous email account

--- Jane.LaGoy-at-bodycote.com wrote:

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} I was enjoying the IQ discourse when it was
} light-hearted, but I don't
} believe the Microscopy Listserver, or any other
} professional listserver,
} should disparage people personally. It is not a
} question of censorship but
} of human decency. What is it our parents said? "If
} you can't say something
} nice about someone then don't say anything at all".
} The IQ lady publicly
} wrote her opinion, so that leaves those words as
} open game for criticism. I
} think it is unfortunate that Sergey was not allowed
} to make his own choice
} about leaving the list; in that way, he was
} censored. This is my personal
} opinion (and since I submitted it, you folks are all
} welcome to criticize
} it.)
}
} Jane L. LaGoy
} R&D Engineer
} Bodycote HIP
} 155 River Street
} Andover, MA 01810
}
}
} ==============================Original
} Headers==============================
} 3, 15 -- From Jane.LaGoy-at-bodycote.com Wed Jul 27
} 08:47:35 2005
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} 3, 15 -- To: "Microscopy Listserver (E-mail)"
} {Microscopy-at-MSA.Microscopy.com}
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5, 19 -- From hoffpajo-at-yahoo.com Thu Jul 28 07:34:04 2005
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 07:39:34 -0500
Subject: [Microscopy] my $0.02 worth on disparagement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

opps i was trying to send that one directly to the
poster, heaven forbit i start a new thread, my
apology.

--- hoffpajo-at-yahoo.com wrote:

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} there is nothing to criticize, Sergey was censored.
} john from an anaoymous email account
}
} --- Jane.LaGoy-at-bodycote.com wrote:
}
} }
} }
} }
} }
}
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} }
} } I was enjoying the IQ discourse when it was
} } light-hearted, but I don't
} } believe the Microscopy Listserver, or any other
} } professional listserver,
} } should disparage people personally. It is not a
} } question of censorship but
} } of human decency. What is it our parents said?
} "If
} } you can't say something
} } nice about someone then don't say anything at
} all".
} } The IQ lady publicly
} } wrote her opinion, so that leaves those words as
} } open game for criticism. I
} } think it is unfortunate that Sergey was not
} allowed
} } to make his own choice
} } about leaving the list; in that way, he was
} } censored. This is my personal
} } opinion (and since I submitted it, you folks are
} all
} } welcome to criticize
} } it.)
} }
} } Jane L. LaGoy
} } R&D Engineer
} } Bodycote HIP
} } 155 River Street
} } Andover, MA 01810
} }
} }
} } ==============================Original
} } Headers==============================
} } 3, 15 -- From Jane.LaGoy-at-bodycote.com Wed Jul 27
} } 08:47:35 2005
} } 3, 15 -- Received: from Exchange.BODYCOTE-IMT.COM
} } (mail.bodycote-imt.com [12.30.23.178])
} } 3, 15 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id j6RDlYof028763
} } 3, 15 -- for {Microscopy-at-MSA.Microscopy.com} ;
} Wed,
} } 27 Jul 2005 08:47:35 -0500
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} }
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} } 3, 15 -- From: JLaGoy {Jane.LaGoy-at-bodycote.com}
} } 3, 15 -- To: "Microscopy Listserver (E-mail)"
} } {Microscopy-at-MSA.Microscopy.com}
} } 3, 15 -- Subject: my $0.02 worth on disparagement
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} 2005
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} 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with



From: hinmeigeng-at-hotmail.com
Date: Thu, 28 Jul 2005 09:13:10 -0500
Subject: [Microscopy] RE: saturated NaOH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

* * * * * * * * *
"I'm trying to express in grams the amount of NaOH that I'd need for 100 ml
of saturated NaOH in ethanol."
* * * * * * * * *
(1) I don't know the exact answer off-hand, but my first guess would be 20
grams in 100 ml ethanol.

(2) However, I do have experience of dissolving alkalies in various alcohols
to make etchants for micrscopy, and there are some things to watch out for:

(a) there will probably be a sodium carbonate crust which will not dissolve.

(b) the behaviour will be very dependent on the amount of water in the
ethanol. In fact, there is a possibility of phase separation into a strong
aqueous NaOH solution and the production of sodium ethoxide in the ethanolic
layer. Regarding ethanol, I read it on the web, but I do have experience
with the same reaction happening in butanol.

*** Of general interest ***

Potassium hydroxide (10% wt/vol) in isopropanol (propan-2-ol) is a jolly
good cleaner for getting charred organic residues off metals and glass. But
don't let it anywhere near aluminium, or you'll start an involuntary
preparation of aluminium isopropoxide.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



==============================Original Headers==============================
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From: TindallR-at-missouri.edu
Date: Thu, 28 Jul 2005 10:36:07 -0500
Subject: [Microscopy] TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Does anyone have experience with acetonitrile as a substitute transition
solvent for PO, or perhaps as the sole dehydration solvent in TEM prep?
I am checking the literature and archives, but any personal experiences
would be very useful.

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







==============================Original Headers==============================
9, 23 -- From TindallR-at-missouri.edu Thu Jul 28 10:36:07 2005
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From: baskin-at-bio.umass.edu
Date: Thu, 28 Jul 2005 10:41:53 -0500
Subject: [Microscopy] Re: TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,
I tried dehydrating plant tissue for LM with acetonitrile
instead of ethanol and the results were terrible. The tissue was
preserved poorly. It was the root of arabidopsis.
Tobias

} ----------------------------------------------------------------------------
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--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
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From: AllanWojtasP-at-AGR.GC.CA
Date: Thu, 28 Jul 2005 11:00:02 -0500
Subject: [Microscopy] TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Randy,

I'd be interested, too, if you would share responses which aren't posted to the list.

Thanks in advance.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure/Chercheure scientifique, microstructure des aliments
Food Safety and Quality Team/Salubrité et qualité des aliments
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
32 Main Street/ 32 rue Main
Kentville, Nova Scotia/Kentville, (Nouvelle-Écosse)
Canada B4N 1J5
Telephone/Téléphone: (902) 679-5566
Facsimile/Télécopieur: (902) 679-2311

allanwojtasp-at-agr.gc.ca


Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Thursday, July 28, 2005 12:37 PM
To: Allan-Wojtas, Paula

Hi all,

Does anyone have experience with acetonitrile as a substitute transition
solvent for PO, or perhaps as the sole dehydration solvent in TEM prep?
I am checking the literature and archives, but any personal experiences
would be very useful.

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







==============================Original Headers==============================
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From: AllanWojtasP-at-AGR.GC.CA
Date: Thu, 28 Jul 2005 11:03:55 -0500
Subject: [Microscopy] sorry for the post

Contents Retrieved from Microscopy Listserver Archives
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Hi, all,
 
I did it too, sent something to the wrong place......I'm sorry. Just getting ready to go on holidays and I acted in haste......
 
P.
 
Paula Allan-Wojtas
Research Scientist - Food Microstructure/Chercheure scientifique, microstructure des aliments
Food Safety and Quality Team/Salubrité et qualité des aliments
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
32 Main Street/ 32 rue Main
Kentville, Nova Scotia/Kentville, (Nouvelle-Écosse)
Canada B4N 1J5
Telephone/Téléphone: (902) 679-5566
Facsimile/Télécopieur: (902) 679-2311
 
allanwojtasp-at-agr.gc.ca
 
 

 


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From: lamiller-at-uiuc.edu
Date: Thu, 28 Jul 2005 12:34:26 -0500
Subject: [Microscopy] Re: TEM: dehydration solvents

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I've found acetonitrile as a PO substitution, to work very well. Also,
it dissolves some plastics less readily, and is better for cell
cultures.

The results seem just as good if not better than PO in my situations. I
don't even use PO anymore.





On Jul 28, 2005, at 10:37 AM, TindallR-at-missouri.edu wrote:
} ----------------------------------------------------------------
}
} Hi all,
}
} Does anyone have experience with acetonitrile as a substitute
} transition
} solvent for PO, or perhaps as the sole dehydration solvent in TEM prep?
} I am checking the literature and archives, but any personal experiences
} would be very useful.
}
} Thanks!
}
} Randy


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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 12:35:34 -0500
Subject: [Microscopy] TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 12:36:14 -0500
Subject: [Microscopy] Re: TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: gvrdolja-at-nature.berkeley.edu
Date: Thu, 28 Jul 2005 14:09:45 -0500
Subject: [Microscopy] 200kv TEM and field emission

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Hello,
I've been normally using a 120kV TEM with a LaB6 filament to image
biological materials. I was using a 200kV tem with a field emission gun
recently and had problems with damage to the samples embedded in epon
araldite and formvar-C TEM grids. Is it necessary to use a different
embedding media with higher emission microscopes?
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 14:10:53 -0500
Subject: [Microscopy] Re: 200kv TEM and field emission

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: tina-at-pbrc.hawaii.edu
Date: Thu, 28 Jul 2005 14:38:29 -0500
Subject: [Microscopy] Bus change for M&M Golf outing

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Hi, Listers-

I apologize for posting to all, but this is the only way we can get this
message to the participants in the M&M 2005 golf outing this weekend. The
computer of the organizer, Mark Sanders, is taking a vacation!

The bus situation has changed. Buses will *not* pick up participants at
the Hawaii Convention Center, but will now pickup at the Sheraton Waikiki
at 9:45 am and at the Hyatt Regency at 10:15, expected to get to Koolau
Golf Course at about 11:15 for a noon tee time.

There are still a few openings for this outing on Saturday and on Sunday;
if you are interested in joing them contact Mark Sanders via cell phone
612-867-5885 or by email when his computer is fixed, msanders-at-cbs.umn.edu

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 14:39:14 -0500
Subject: [Microscopy] Re: Bus change for M&M Golf outing

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: dmclea-at-sandia.gov
Date: Thu, 28 Jul 2005 14:48:55 -0500
Subject: [Microscopy] M&M in Hawaii

Contents Retrieved from Microscopy Listserver Archives
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With you all flitting off to Hawaii could you PLEASE do me a favor and
not rub my nose in the fact that ya'll get to go on vacation and I have
to stay home. Please don't send the "LISTSERVER" an automatic "out of
office reply". I've worn the writing off my delete key this week.

Thanks very much,

Dorrance McLean
Intrepid GIRL scientist





Dorrance McLean
Microsystems Processing
Sandia National Laboratories
P.O. Box 969, MS 9401
Livermore, CA 94551-0969
925-294-3551 FAX 3870
Email dmclea-at-sandia.gov



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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 14:50:42 -0500
Subject: [Microscopy] Re: University facilities being used for outside users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hi Pat,

You have described what, IMHO, and probably that of most others, is a model
policy for appropriate use of university facilities.

Actually there are, within 100 miles of Philadelphia, probably half a dozen
for-profit independent laboratories focused (no pun intended) on microscopy
capabilities. Some of them are listed on our URL
http://www.2spi.com/catalog/hot-service7.html One of our main competitors
for the services is EMSL, right across the river from you in Camden.

There is nothing to my knowledge that says a nonprofit can not offer
services per se and receive a payment. But you have to become knowledgeable
about the tax code itself, and understand how the different classes of tax-
exempt institutions differ. Universities are in a separate category and are
restricted to doing those things that enhance educational objectives. When
the Congress some years ago recognized that some tax-exempt organizations
might engage in business/commercial activities, they enacted the UBIT
(unrelated business income tax). It was supposed to be the equalizer that
kept competition fair between such organizations (mainly not-for-profits or
so-called Section 501-(c)-3 organizations). It never worked very well (for
what for some could be interesting reasons), but the point was, UBIT was
never applied to those operating under the university exemption because
Congress just did not envision that a university ever would be engaging in a
business/commercial activity. In other words, even if a university
**wanted** to pay tax on some activity, there is absolutely no provision in
the current tax code for such a tax to be collected and paid!

The Franklin Institute case, to which you referred, was a special case. To
explain what happened would require a posting so long it would probably
cause Nestor's filters to see it as SPAM..... But they were a not-for-
profit (as opposed to universities in general being non-profits)
organization and they literally tried to drive Structure Probe, Inc. out of
the marketplace by offering rates for SEM services in the early 1970's so
low that no for-profit firm could survive. We were the plaintiff who filed
an anti-trust suit against them in 1972.

What did them in, and the main reason why their whole research laboratory is
no more is that their management people, at the trial and when under oath,
were saying things considerably different from when they were talking
informally to the IRS and others, like the newspapers. And the net result
was that the Franklin Institute Research Laboratories had to become Franklin
Institute Research Laboratories, Inc. And this meant they lost most of the
benefits they enjoyed as a not-for-profit tax-exempt organization. Putting
it another way, they had to pay the same taxes as any other business, they
had to double or triple their charge rates and once they started doing that,
they started losing customers, and their financial losses became a
hemorrhage (literally) and within a few years, they closed up shop and
disappeared.

I have given somewhat of a synopsis as to what happened but presumably the
court documents and filings are still part of the public record somewhere
and if one was interested, such information should be available should one
be interested in learning more.

What made this situation unique is that most institutional administrations
when confronted with an errant department engaging in inappropriate
activities on such a wholesale scale, would step in and say "whoaaaa" and
put a stop to it. But in this case, the officers and managers thought they
could literally run a tiny three person company out of money and therefore
out of business. They obviously failed, but because of that decision,
something like 600+ persons eventually lost their jobs when the FIRL, Inc.
closed down. The irony to me was that the officers and managers in the end
lost nothing. Those from prestigious Philadelphia industrial firms who sat
on their Board of Managers lost nothing. None of them were ever held
accountable. They were never punished. They just rode off into the sunset
with golden glove handshakes......

So that, in a nut shell, is the Philadelphia Story...... and the demise of
the Franklin Institute Research Laboratories.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================






----- Original Message -----
X-from: Pat Connelly
To: microscopy-at-microscopy.com
Cc: cgarber-at-2spi.com
Sent: Thursday, July 28, 2005 1:34 PM

I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: tivol-at-caltech.edu
Date: Thu, 28 Jul 2005 14:54:22 -0500
Subject: [Microscopy] Re: 200kv TEM and field emission

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jul 28, 2005, at 12:09 PM, gvrdolja-at-nature.berkeley.edu wrote:

} I've been normally using a 120kV TEM with a LaB6 filament to image
} biological materials. I was using a 200kV tem with a field emission
} gun
} recently and had problems with damage to the samples embedded in epon
} araldite and formvar-C TEM grids. Is it necessary to use a different
} embedding media with higher emission microscopes?
}
Dear Gordon,
Unless you were operating the 200 kV FEG at a much higher intensity
than the 120 kV LaB6, you shouldn't experience damage problems. One
can always use a lower-intensity beam (larger spot size number) and
spread the beam over a wide area so that a FEG will deliver a low dose
rate to the specimen. If you have an intense beam and a
correspondingly short exposure for your image, the beam can do damage
during the time that it is on the specimen but the image is not being
exposed. To clarify that last sentence, if you're scanning the grid
with the LaB6 beam, imaging with a 1 sec exposure, and you experience
no significant damage during the (say) 10-20 sec during which you're
focussing, framing the image, etc., then you go to a FEG beam with a
0.1 sec exposure, taking the same length of time to focus, etc., you
will have exposed your specimen to 10 times the dose, so there may well
be unacceptable damage. Otherwise, the 200 kV beam does less damage
per unit dose than the 120 kV beam, and there is nothing inherent in
the FEG that will increase the damage. Standard techniques for
producing plastic-embedded specimens should work as well for the 200 kV
FEG as for the 120 kV LaB6.
Yours,
Bill



==============================Original Headers==============================
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5, 26 -- Subject: Re: [Microscopy] 200kv TEM and field emission
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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 14:55:30 -0500
Subject: [Microscopy] 200kv TEM and field emission

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: bmollon-at-pacbell.net
Date: Thu, 28 Jul 2005 15:20:22 -0500
Subject: [Microscopy] rules

Contents Retrieved from Microscopy Listserver Archives
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In the U.S., the sky is always blue.

I care about people and would never use the remark of
"I dont care about you". Its a simple polite,
compassionate rule that most of us are taught to care
for people. My point was it was a poor choice of
words. Take out that sentence and I think you'll see
Nestor would still make his point without sharing his
frustrating remark of caring.

Im not going to add any remarks on the subject of this
thread. It's already contorted beyond intent of the
original author.
My posting was an observation as to how everyone,
including the sysop, got carried away with this
originally intended "amusement post" and maybe all of
us ended up being guilty of stepping on the rules.
Again, no reason to publically announce how much you
care about someone.

Anonymous posting? What more do you need besides my
email address and that Im a member of this list? Let
me know what you need to know.



--- r.sims-at-auckland.ac.nz wrote:

}
}
}
}
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}
}
} Dear bmollon
}
}
} How on earth do you read Nestor's email as
} "derogatory and demeaning"?
}
} Do you see some equivalence between saying "I don't
} care who you are" and
} describing someone else as practising "crap
} journalism"?
}
} What color is the sky in your world?
}
} And how about having the courage to identify
} yourself?
}
} Anonymous postings don't rate very highly with most
} people.
}
} cheers
}
} rtch
}
}
}
}
} Date sent: Wed, 27 Jul 2005 09:42:24 -0500
} To: r.sims-at-auckland.ac.nz
} X-from: bmollon-at-pacbell.net
} Send reply to: microscopy-at-microscopy.com
} Subject: [Microscopy] rules
}
} }
} }
} }
} }
}
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} }
} } Nestor..........since you just used a derogatory
} and
} } demeaning statement about someone publically using
} the
} } listserver........are you going to remove yourself
} } from the list? I think the "I dont care who you
} are"
} } could have been left off and your point still
} made.
} }
} } Nestor wrote:
} } "Sergy
} }
} } I don't care who you are or where your from, but I
} } insist the rules
} } which have
} } been established for over a decade are followed by
} } all. Since you
} } believe you are above the rules so be it. You are
} } welcome to
} } go elsewhere.
} }
} } I have canceled your subscription to the
} Listserver.
} }
} }
} } Nestor
} } Microscopy SysOp"
} }
} } --
} Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
} Microanalyst Fax : 64 9
} 3737435
} Department of Geology email :
} r.sims-at-auckland.ac.nz
} The University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
}
} ==============================Original
} Headers==============================
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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 15:21:16 -0500
Subject: [Microscopy] Re: rules

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: William.Giles-at-TIMET.com
Date: Thu, 28 Jul 2005 15:33:42 -0500
Subject: [Microscopy] Out of office replies and chat room style posts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Seems I'm being besieged by out of office messages, I'm glad your in Hawaii
but don't rub it in.

And to the chat room drama that has filled my mail box, come on guys take it
somewhere else.


*

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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 15:34:32 -0500
Subject: [Microscopy] Re: Out of office replies and chat room style posts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be out of the office from July 28th returning to the office on August 8th, 2005.

==============================Original Headers==============================
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From: dmclea-at-sandia.gov
Date: Thu, 28 Jul 2005 15:48:52 -0500
Subject: [Microscopy] Re: Out of office replies and chat room style

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If this doesn't stop I'll never buy another JEOL product...pcorkum has
filled my inbox with automatic out of office replies! And of course,
I'll just get another one bounced form complaining! Sorry...I just had
to vent.

Dorrance McLean

-----Original Message-----
X-from: pcorkum-at-jeol.com [mailto:pcorkum-at-jeol.com]
Sent: Thursday, July 28, 2005 1:35 PM
To: McLean, Dorrance

I will be out of the office from July 28th returning to the office on
August 8th, 2005.

==============================Original
Headers==============================
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==============================Original Headers==============================
11, 31 -- From dmclea-at-sandia.gov Thu Jul 28 15:48:51 2005
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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 16:29:09 -0500
Subject: [Microscopy] Re: Out of office replies and chat room style

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Thu, 28 Jul 2005 15:49:22 -0500
dmclea-at-sandia.gov wrote:
} Hello,

My most humble apologies to all!
This was a dreadful oversight on my part, and will be
corrected as promptly as possible.

I truly am sorry about the confusion.

Most cordially,
Patricia Corkum
JEOL USA
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America


==============================Original Headers==============================
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From: dbaldwin-at-dgisrd.com
Date: Thu, 28 Jul 2005 19:12:49 -0500
Subject: [Microscopy] TEM/SEM Employment Opportunity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK (RTP), NORTH
CAROLINA AREA

We have an immediate need for a full-time Electron Microscope Technician
with solid knowledge of maintenance and operation. 3+ years TEM and SEM
experience in the maintenance and operations of electron microscopy
required.

Send resume and salary requirements to sjeffers-at-dgisrd.com
{mailto:sjeffers-at-dgisrd.com} .



==============================Original Headers==============================
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From: gary-at-gaugler.com
Date: Thu, 28 Jul 2005 20:37:07 -0500
Subject: [Microscopy] Zeiss drift correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Has anyone been able to get the Zeiss/LEO drift
correction option to work? I have the license
and the dongle. Nevertheless, the feature seems
rather dysfunctional...or I don't know how to use it.

Can someone enlighten me about this option? I can
really use it for long data capture sessions combined
with the AVI capture option.

gary g.


==============================Original Headers==============================
4, 16 -- From gary-at-gaugler.com Thu Jul 28 20:37:07 2005
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From: ferretinmicrowave-at-ns.microscopy.com
Date: Thu, 28 Jul 2005 21:06:22 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow flake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ferretinmicrowave) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Sunday, July 24, 2005 at 14:38:34
---------------------------------------------------------------------------

Email: ferretinmicrowave
Name: Isabel Verde

Organization: University of Pennsylvania

Education: 9-12th Grade High School

Location: Chicago, IL, USA

Question: How do you take an image of a snowflake with an optical
microscope? What sort of special techniques and equipment do you
need in order to take the picture?

---------------------------------------------------------------------------

==============================Original Headers==============================
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7, 12 -- From: ferretinmicrowave-at-ns.microscopy.com (by way of Ask-A-Microscopist)
7, 12 -- Subject: [Filtered] AskAMicroscopist: How to image a snow flake
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ballardmark-at-gmail.com
Date: Thu, 28 Jul 2005 21:07:01 -0500
Subject: [Microscopy] AskAMicroscopist: an affordable student microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ballardmark-at-gmail.com) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Thursday, July 28, 2005 at 10:58:06
---------------------------------------------------------------------------

Email: ballardmark-at-gmail.com
Name: Marcello

Organization: None

Education: 9-12th Grade High School

Location: Orlando, Florida

Question: Hi to all,

i am trying to buy the most afforable microscopy, but that will be
able to help me with biology and chemistry.

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Thu Jul 28 21:07:01 2005
8, 12 -- Received: from [10.0.3.241] (msdvpn24.msd.anl.gov [130.202.238.88])
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8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: ballardmark-at-gmail.com (by way of Ask-A-Microscopist)
8, 12 -- Subject: AskAMicroscopist: an affordable student microscope
8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dbaldwin-at-dgisrd.com
Date: Thu, 28 Jul 2005 21:09:44 -0500
Subject: [Microscopy] viaWWW: TEM/SEM Employment Opportunity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dbaldwin-at-dgisrd.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Thursday, July 28, 2005 at 13:35:21
---------------------------------------------------------------------------

Email: dbaldwin-at-dgisrd.com
Name: DBaldwin

Organization: BioWarn, LLC

Title-Subject: [Filtered] TEM/SEM Employment Opportunity

Question: ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK
(RTP), NORTH CAROLINA AREA

We have an immediate need for a full-time Electron Microscope
Technician with solid knowledge in maintenance and operation. 3+
years TEM and SEM experience in maintenance and operation of electron
microscopy required.

Send resume and salary requirements to: sjeffers-at-dgisrd.com.



---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Thu Jul 28 21:09:44 2005
10, 12 -- Received: from [10.0.3.241] (msdvpn24.msd.anl.gov [130.202.238.88])
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10, 12 -- From: dbaldwin-at-dgisrd.com (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: TEM/SEM Employment Opportunity
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: wpchan-at-u.washington.edu
Date: Thu, 28 Jul 2005 21:13:01 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Isabel

You might want to check out this site.

http://www.its.caltech.edu/~atomic/snowcrystals/photo2/photo2.htm

--
Pang (Wai Pang Chan, wpchan-at-u.washington.edu)

On Thu, 28 Jul 2005 ferretinmicrowave-at-ns.microscopy.com wrote:

} Email: ferretinmicrowave
} Name: Isabel Verde
}
} Organization: University of Pennsylvania
}
} Education: 9-12th Grade High School
}
} Location: Chicago, IL, USA
}
} Question: How do you take an image of a snowflake with an optical
} microscope? What sort of special techniques and equipment do you
} need in order to take the picture?

==============================Original Headers==============================
6, 21 -- From wpchan-at-u.washington.edu Thu Jul 28 21:13:00 2005
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6, 21 -- From: "W. Chan" {wpchan-at-u.washington.edu}
6, 21 -- To: microscopy-at-microscopy.com
6, 21 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow
6, 21 -- flake
6, 21 -- In-Reply-To: {200507290209.j6T29dqj001639-at-ns.microscopy.com}
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 21:27:33 -0500
Subject: [Microscopy] Re: AskAMicroscopist: an affordable student microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

have you gone through your purchasing dept?

--- ballardmark-at-gmail.com wrote:

}
}
}
}
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} Below is the result of your feedback form
} (NJZFM-ultra-55). It was
} submitted by (ballardmark-at-gmail.com) from
}
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
}
} on Thursday, July 28, 2005 at 10:58:06
}
---------------------------------------------------------------------------
}
} Email: ballardmark-at-gmail.com
} Name: Marcello
}
} Organization: None
}
} Education: 9-12th Grade High School
}
} Location: Orlando, Florida
}
} Question: Hi to all,
}
} i am trying to buy the most afforable microscopy,
} but that will be
} able to help me with biology and chemistry.
}
}
---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Thu Jul 28
} 21:07:01 2005
} 8, 12 -- Received: from [10.0.3.241]
} (msdvpn24.msd.anl.gov [130.202.238.88])
} 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j6T26vm7024730
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} 8, 12 -- To: microscopy-at-microscopy.com
} 8, 12 -- From: ballardmark-at-gmail.com (by way of
} Ask-A-Microscopist)
} 8, 12 -- Subject: AskAMicroscopist: an affordable
} student microscope
} 8, 12 -- Content-Type: text/plain;
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} Headers==============================
}


__________________________________________________
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==============================Original Headers==============================
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] AskAMicroscopist: an affordable student microscope
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From: r.sims-at-auckland.ac.nz
Date: Fri, 29 Jul 2005 00:20:21 -0500
Subject: [Microscopy] AskAMicroscopist: an affordable student

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well said, John!

I'm sure that the enquiring high school student will find the information you
have so thoughtfully and kindly supplied to him to be useful, will encourage his
interest in microscopy, and will induce in him a new respect for microscopists.

Marcello, please wait a little longer and you may find that other list members
may post for you information even more relevant and useful than John's kind reply.

I would try, but what I know about light microscopy could easily be written on a
small Post-It note.

cheers

Ritchie Sims
Auckland
New Zealand


Quoting hoffpajo-at-yahoo.com:

}
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} } submitted by (ballardmark-at-gmail.com) from
} }
} http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
} }
} } on Thursday, July 28, 2005 at 10:58:06
} }
} ---------------------------------------------------------------------------
} }
} } Email: ballardmark-at-gmail.com
} } Name: Marcello
} }
} } Organization: None
} }
} } Education: 9-12th Grade High School
} }
} } Location: Orlando, Florida
} }
} } Question: Hi to all,
} }
} } i am trying to buy the most afforable microscopy,
} } but that will be
} } able to help me with biology and chemistry.
} }
} }



-------------------------------------------------
This mail sent through University of Auckland
http://www.auckland.ac.nz/

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From: bbuenaobra-at-nip.upd.edu.ph
Date: Fri, 29 Jul 2005 06:27:20 -0500
Subject: [Microscopy] RE: AskAMicroscopist: an affordable student

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Isabel

Some cameras, such as the Nikon 4500 can image larger snow flakes directly
with their macro modes,
but individual crystals normally require a microscope or a powerful macro
setup.
Any stereomicroscope with camera attached will do the job, or you could
point a digital camera down the eyepiece of any suitable microscope. Either
transmitted brightfield
or darkfield illumination will work, and imaging between crossed polarisers
can generate
interesting colour effects.

The main problem is to prevent the snowflake from melting, so
the microscope slide and stage need to be at the same temperature as the
snowflake.
The simplest way, if not the most comfortable way, to do this is to work
outside in the snow-shower,
or in an unheated shed at subzero temperature.

Some tips, images and historical background can be had from this site:
http://www.its.caltech.edu/~atomic/snowcrystals/

With global warming this winter may be your last opportunity...
Wrap up warm
Chris



----- Original Message -----
X-from: {ferretinmicrowave-at-ns.microscopy.com}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Friday, July 29, 2005 3:06 AM


Would the Intel-Mattel microscope with a USB support be available?
That for high school could really be affordable.

Berns


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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Jul 2005 08:25:10 -0500
Subject: [Microscopy] re: An affordable student microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Check out the Surplus Shed at www.surplusshed.com. They have both
dissecting stereomicroscope and compound microscope for $ 95.00 each. I
can't vouch for their quality since I've not bought one, but the price is
right, and I'm satisfied with other things I've purchased from them. Do NOT
buy a discount chain store microscope. They're junk.

Paul Grover


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln




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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Jul 2005 08:35:59 -0500
Subject: [Microscopy] re: how to image a snowflake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Isabel,

You might want to consider making plastic snowflake replicas. Just google
'snowflake replicas' and you'll find a bunch of sites. If you have trouble
finding Formvar, contact me and I'll send you some.

I've had great fun coming inside from a snowstorm and passing these around
to guests at a party and seeing how long it takes them to figure out that
the snowflakes aren't melting indoors.

Paul Grover


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln



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From: ekman-at-itg.uiuc.edu
Date: Fri, 29 Jul 2005 08:40:06 -0500
Subject: [Microscopy] cameras in labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When I was at Florida State University the I.T. people in the Biology
Department placed networked cameras in the student computer labs.

http://www.axis.com/products/cam_210/index.htm

They had them saving a still pictures every 5 seconds to a network
server. I think you can capture video as well with the 210 which is linked
above.

Neat thing was that the large format poster printer was in view in one lab
so you could just check the web address of the camera to see if your poster
was done printing without leaving your office.

Once installed we built metal cages to protect them from being stolen.

I have no financial ties to the company above, just a product that I have
worked with in the past with good success.

Hope this helps,

Jon Ekman
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 N. Mathews Avenue
Urbana, IL 61801 USA
Tel: 217-244-6292
Fax: 217-244-6219

-----Original Message-----
X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
Sent: Wednesday, July 27, 2005 1:38 PM
To: ekman-at-itg.uiuc.edu

Hi everyone,

We are currently considering adding security cameras in each of our EM
labs.

We have a very large number of users, both during working hours and
after-hours in SEM and TEM rooms. Just recently, we have had a few
incidents where people have damaged the instrument and did not come
forward to admit it.

Although we can see who logged on to the computers, you can still damage
the instrument without logging in. We believe that a CCTV in each of the
labs would be the best way to solve this problem, but unfortunately we
are faced with "privacy issues". People do not want to be watched.

Please tell me if your labs use cameras (live or log) or if you found
other ways to get around the problem.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632


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From: TindallR-at-missouri.edu
Date: Fri, 29 Jul 2005 08:46:17 -0500
Subject: [Microscopy] AskAMicroscopist: an affordable student microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Marcello,

Although I have no experience with them, I have received a catalog from
a company called Walter Products, Inc.based in Ontario. They specialize
in scientific equipment, including microscopes, telescopes, and other
supplies. Although the catalog doesn't list their prices for
microscopes, their telescope prices seem to be amazingly reasonable
(i.e., low) and their warranties seem fine.

You might want to contact them. The email is walter1-at-on.aibn.com, and
phone is 519-737-7901. Google the name to get their website. I have no
connection or experiences with them, but it might be worth a look.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
X-from: ballardmark-at-gmail.com [mailto:ballardmark-at-gmail.com]
Sent: Thursday, July 28, 2005 9:08 PM
To: Tindall, Randy D.

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ballardmark-at-gmail.com) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Thursday, July 28, 2005 at 10:58:06
------------------------------------------------------------------------
---

Email: ballardmark-at-gmail.com
Name: Marcello

Organization: None

Education: 9-12th Grade High School

Location: Orlando, Florida

Question: Hi to all,

i am trying to buy the most afforable microscopy, but that will be able
to help me with biology and chemistry.

------------------------------------------------------------------------
---

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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Jul 2005 08:48:35 -0500
Subject: [Microscopy] snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

And be sure to check out Wilson A. Bentley's life & work. He pioneered
snowflake photography a long time ago, and did it the hard way. Amateurs
rock!

Paul Grover

------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln



==============================Original Headers==============================
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5, 23 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu}
5, 23 -- To: {microscopy-at-microscopy.com}
5, 23 -- Subject: snowflakes
5, 23 -- Date: Fri, 29 Jul 2005 08:48:35 -0500
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From: gstrout-at-ou.edu
Date: Fri, 29 Jul 2005 08:55:06 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow flake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Isabel,

Hava a look at snowcrystals.com it is a site with lots of info and links
about snowflakes including photography.




ferretinmicrowave-at-ns.microscopy.com wrote:

} ----------------------------------------------------------------------------
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--
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




==============================Original Headers==============================
11, 22 -- From gstrout-at-ou.edu Fri Jul 29 08:55:06 2005
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11, 22 -- From: Greg Strout {gstrout-at-ou.edu}
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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Jul 2005 09:35:57 -0500
Subject: [Microscopy] raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Esteemed Microscopists,

Since most of you apparently are in Hawaii now, I'll waste a little
bandwidth and a few KB to vent. I'd like to note that:

(1) This is a MICROSCOPY listserver. This apparently means "looking at
little things".

(2) There is apparently no requirement that one must be using the latest
multi-probe cutting edge technology, run a multi-user facility, or have a
purchasing department, federal grant, or even a job. Our common bond is
that we like to "look at little things".

(3) The greatest scientists have been, mostly, AMATEURS (from Latin
'amator', i.e. someone who does something out of love instead of for
financial gain). As we celebrate the centenary of Einstein's 'miracle year'
which changed our concept of the universe, let's remember that he did this
work as an amateur.

(4) In a few years we'll all be toothless curmudgeons and today's
schoolkids will be deciding whether Medicare covers immersion oil and cover
slips.

(5) I like to "look at little things" but I'm a carpenter by trade. If you
don't like that, bite me.

(6) If you aren't reading this it's because you're in Hawaii and I hate you
because I'm not.


Paul Grover :0)


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln




==============================Original Headers==============================
15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 09:35:57 2005
15, 24 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130])
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15, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu}
15, 24 -- To: {microscopy-at-microscopy.com}
15, 24 -- Subject: raison d' etre
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 10:20:16 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

very well said, and Hawaii is over rated try St
Martins, much better. you must be the one with the
Highest IQ in here.
john

--- pgrover-at-bilbo.bio.purdue.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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} On-Line Help
}
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}
----------------------------------------------------------------------------
}
} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll
} waste a little
} bandwidth and a few KB to vent. I'd like to note
} that:
}
} (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one
} must be using the latest
} multi-probe cutting edge technology, run a
} multi-user facility, or have a
} purchasing department, federal grant, or even a job.
} Our common bond is
} that we like to "look at little things".
}
} (3) The greatest scientists have been, mostly,
} AMATEURS (from Latin
} 'amator', i.e. someone who does something out of
} love instead of for
} financial gain). As we celebrate the centenary of
} Einstein's 'miracle year'
} which changed our concept of the universe, let's
} remember that he did this
} work as an amateur.
}
} (4) In a few years we'll all be toothless
} curmudgeons and today's
} schoolkids will be deciding whether Medicare covers
} immersion oil and cover
} slips.
}
} (5) I like to "look at little things" but I'm a
} carpenter by trade. If you
} don't like that, bite me.
}
} (6) If you aren't reading this it's because you're
} in Hawaii and I hate you
} because I'm not.
}
}
} Paul Grover :0)
}
}
}
------------------------------------------------------------------------
} No matter how much cats fight, there always seem to
} be plenty of kittens.
} - A.
} Lincoln
}
}
}
}
} ==============================Original
} Headers==============================
} 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul
} 29 09:35:57 2005
} 15, 24 -- Received: from mailhub130.itcs.purdue.edu
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} {pgrover-at-bilbo.bio.purdue.edu}
} 15, 24 -- To: {microscopy-at-microscopy.com}
} 15, 24 -- Subject: raison d' etre
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==============================Original Headers==============================
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] raison d' etre
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5, 19 -- In-Reply-To: {200507291437.j6TEbevE011473-at-ns.microscopy.com}
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 10:20:55 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

very well said, and Hawaii is over rated try St
Martins, much better. you must be the one with the
Highest IQ in here.
john


--- pgrover-at-bilbo.bio.purdue.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll
} waste a little
} bandwidth and a few KB to vent. I'd like to note
} that:
}
} (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one
} must be using the latest
} multi-probe cutting edge technology, run a
} multi-user facility, or have a
} purchasing department, federal grant, or even a job.
} Our common bond is
} that we like to "look at little things".
}
} (3) The greatest scientists have been, mostly,
} AMATEURS (from Latin
} 'amator', i.e. someone who does something out of
} love instead of for
} financial gain). As we celebrate the centenary of
} Einstein's 'miracle year'
} which changed our concept of the universe, let's
} remember that he did this
} work as an amateur.
}
} (4) In a few years we'll all be toothless
} curmudgeons and today's
} schoolkids will be deciding whether Medicare covers
} immersion oil and cover
} slips.
}
} (5) I like to "look at little things" but I'm a
} carpenter by trade. If you
} don't like that, bite me.
}
} (6) If you aren't reading this it's because you're
} in Hawaii and I hate you
} because I'm not.
}
}
} Paul Grover :0)
}
}
}
------------------------------------------------------------------------
} No matter how much cats fight, there always seem to
} be plenty of kittens.
} - A.
} Lincoln
}
}
}
}
} ==============================Original
} Headers==============================
} 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul
} 29 09:35:57 2005
} 15, 24 -- Received: from mailhub130.itcs.purdue.edu
} (mailhub130.itcs.purdue.edu [128.210.5.130])
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} with ESMTP id j6TEZvKW008928
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} Jul 2005 09:35:57 -0500
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} (dhcp155-220.bio.purdue.edu [128.210.155.220])
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} Jul 2005 09:35:57 -0500
} 15, 24 -- From: "pgrover"
} {pgrover-at-bilbo.bio.purdue.edu}
} 15, 24 -- To: {microscopy-at-microscopy.com}
} 15, 24 -- Subject: raison d' etre
} 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500
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} {000101c5944a$d5416290$dc9bd280-at-paklabpgrover}
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6, 19 -- Subject: Re: [Microscopy] raison d' etre
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From: microbill-at-mohawk.net
Date: Fri, 29 Jul 2005 10:37:48 -0500
Subject: [Microscopy] raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I fully concur - I'll be there next week

At 11:21 AM 7/29/2005, you wrote:



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==============================Original Headers==============================
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7, 20 -- Date: Fri, 29 Jul 2005 11:37:33 -0400
7, 20 -- From: Bill Miller {microbill-at-mohawk.net}
7, 20 -- Subject: Re: [Microscopy] Re: raison d' etre
7, 20 -- In-reply-to: {200507291521.j6TFLHr7020030-at-ns.microscopy.com}
7, 20 -- To: microscopy-at-microscopy.com
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From: frank.karl-at-degussa.com
Date: Fri, 29 Jul 2005 10:38:10 -0500
Subject: [Microscopy] Fw: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





I find myself perplexed by the reason for Paul Gover’s note. I typically
check titles and skim the content of the note if I’m interested.
Everything else gets trashed canned, so I haven’t been paying too much
attention to the discussion. If my lurking finds a theme I’m interested
in, I’m not above kicking the embers to bring forth light and heat. With
this in mind….


I’ve been making a reasonable living as a microscopist for over 25 years
and I still consider myself a very fortunate amateur. I too like to look
at little things and to that end built a home microscopy lab while I was in
college. I consider the time spent studying diatoms, collecting pollen or
cutting free hand thin section of multi-layer bottles to be golden. I find
that these skills are transferable to the professional arena.

My first position in a multi-used environment was in the tire industry.
Conversations over coffee revealed none of my new co-workers owned a home
lab. My microscopy culture tells me all microscopists have a home lab. I
knew, at that moment, my co-workers were simply employees and nothing more.
All of the microscopist I admire have a home lab and several have created
an employment and provided for the welfare of their family from it. Some
find their professional lives do not provide the luxury of using the home
lab and most regret it.

If amateurs are not welcome on this list, I suggest a name change. I have
found this server a useful tool, but I’ll hate to see it restricted to only
“profession†microscopist.

Frank Karl
Degussa Corporation


.
----- Forwarded by Frank Karl/AKR/Degussa-Huels/US on 07/29/2005 11:34 AM
-----

pgrover-at-bilbo.bio
.purdue.edu To: frank.karl-at-degussa.com
cc:
07/29/2005 10:37 Subject: [Microscopy] raison d' etre
AM
Please respond to
microscopy








----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Esteemed Microscopists,

Since most of you apparently are in Hawaii now, I'll waste a little
bandwidth and a few KB to vent. I'd like to note that:

(1) This is a MICROSCOPY listserver. This apparently means "looking at
little things".

(2) There is apparently no requirement that one must be using the latest
multi-probe cutting edge technology, run a multi-user facility, or have a
purchasing department, federal grant, or even a job. Our common bond is
that we like to "look at little things".

(3) The greatest scientists have been, mostly, AMATEURS (from Latin
'amator', i.e. someone who does something out of love instead of for
financial gain). As we celebrate the centenary of Einstein's 'miracle
year'
which changed our concept of the universe, let's remember that he did this
work as an amateur.

(4) In a few years we'll all be toothless curmudgeons and today's
schoolkids will be deciding whether Medicare covers immersion oil and cover
slips.

(5) I like to "look at little things" but I'm a carpenter by trade. If
you
don't like that, bite me.

(6) If you aren't reading this it's because you're in Hawaii and I hate
you
because I'm not.


Paul Grover :0)


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln






==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 10:46:36 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

enjoy it is a beautiful island where everyone is very
friendly. i may move there full time if i can get the
right price for my house
john

--- microbill-at-mohawk.net wrote:

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}
} I fully concur - I'll be there next week
}
} At 11:21 AM 7/29/2005, you wrote:
}
}
}
}
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} }
} } very well said, and Hawaii is over rated try St
} } Martins, much better. you must be the one with the
} } Highest IQ in here.
} } john
} }
} }
} } --- pgrover-at-bilbo.bio.purdue.edu wrote:
} }
} } }
} } }
} } }
} } }
}
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
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} } }
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} } }
}
} ----------------------------------------------------------------------------
} } }
} } } Esteemed Microscopists,
} } }
} } } Since most of you apparently are in Hawaii now,
} I'll
} } } waste a little
} } } bandwidth and a few KB to vent. I'd like to
} note
} } } that:
} } }
} } } (1) This is a MICROSCOPY listserver. This
} } } apparently means "looking at
} } } little things".
} } }
} } } (2) There is apparently no requirement that one
} } } must be using the latest
} } } multi-probe cutting edge technology, run a
} } } multi-user facility, or have a
} } } purchasing department, federal grant, or even a
} job.
} } } Our common bond is
} } } that we like to "look at little things".
} } }
} } } (3) The greatest scientists have been, mostly,
} } } AMATEURS (from Latin
} } } 'amator', i.e. someone who does something out of
} } } love instead of for
} } } financial gain). As we celebrate the centenary
} of
} } } Einstein's 'miracle year'
} } } which changed our concept of the universe, let's
} } } remember that he did this
} } } work as an amateur.
} } }
} } } (4) In a few years we'll all be toothless
} } } curmudgeons and today's
} } } schoolkids will be deciding whether Medicare
} covers
} } } immersion oil and cover
} } } slips.
} } }
} } } (5) I like to "look at little things" but I'm a
} } } carpenter by trade. If you
} } } don't like that, bite me.
} } }
} } } (6) If you aren't reading this it's because
} you're
} } } in Hawaii and I hate you
} } } because I'm not.
} } }
} } }
} } } Paul Grover :0)
} } }
} } }
} } }
}
} ------------------------------------------------------------------------
} } } No matter how much cats fight, there always seem
} to
} } } be plenty of kittens.
} } } -
} A.
} } } Lincoln
} } }
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri
} Jul
} } } 29 09:35:57 2005
} } } 15, 24 -- Received: from
} mailhub130.itcs.purdue.edu
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} } } Jul 2005 09:35:57 -0500
} } } 15, 24 -- From: "pgrover"
} } } {pgrover-at-bilbo.bio.purdue.edu}
} } } 15, 24 -- To: {microscopy-at-microscopy.com}
} } } 15, 24 -- Subject: raison d' etre
} } } 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500
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} } ==============================Original
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5, 19 -- Subject: Re: [Microscopy] raison d' etre
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From: marc.pypaert-at-yale.edu
Date: Fri, 29 Jul 2005 10:49:02 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paul,

Why perpetuate the myth of the amateur scientist doing his job just for
the love of science and not for money? As far as I know, Einstein made
a pretty good living for himself while teaching at Princeton, and I'm
sure
that great scientists before him, like Louis Pasteur, were not poor
either!
I like many others on this listserver have chosen microscopy as a
career,
as a way to make a living, and the fact that the "little things" that
we look
at every day are so beautiful is just a bonus to our jobs! We're doing
science a disservice if we tell schoolkids that, if they want to make a
living, they should not consider a scientific career!

But like you, I'm pretty jealous of all those going to Hawaii this
weekend!

Marc


On Friday, July 29, 2005, at 10:37 AM, pgrover-at-bilbo.bio.purdue.edu
wrote:

}
}
}
} -----------------------------------------------------------------------
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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} -----
}
} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll waste a little
} bandwidth and a few KB to vent. I'd like to note that:
}
} (1) This is a MICROSCOPY listserver. This apparently means "looking
} at
} little things".
}
} (2) There is apparently no requirement that one must be using the
} latest
} multi-probe cutting edge technology, run a multi-user facility, or
} have a
} purchasing department, federal grant, or even a job. Our common bond
} is
} that we like to "look at little things".
}
} (3) The greatest scientists have been, mostly, AMATEURS (from Latin
} 'amator', i.e. someone who does something out of love instead of for
} financial gain). As we celebrate the centenary of Einstein's 'miracle
} year'
} which changed our concept of the universe, let's remember that he did
} this
} work as an amateur.
}
} (4) In a few years we'll all be toothless curmudgeons and today's
} schoolkids will be deciding whether Medicare covers immersion oil and
} cover
} slips.
}
} (5) I like to "look at little things" but I'm a carpenter by trade.
} If you
} don't like that, bite me.
}
} (6) If you aren't reading this it's because you're in Hawaii and I
} hate you
} because I'm not.
}
}
} Paul Grover :0)
}
}
} -----------------------------------------------------------------------
} -
} No matter how much cats fight, there always seem to be plenty of
} kittens.
} - A. Lincoln
}
}
}
}
} ==============================Original
} Headers==============================
} 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 09:35:57 2005
} 15, 24 -- Received: from mailhub130.itcs.purdue.edu
} (mailhub130.itcs.purdue.edu [128.210.5.130])
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} j6TEZvKW008928
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} -0500
} 15, 24 -- Received: from paklabpgrover (dhcp155-220.bio.purdue.edu
} [128.210.155.220])
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} with ESMTP id j6TEZv5i001612
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} -0500
} 15, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu}
} 15, 24 -- To: {microscopy-at-microscopy.com}
} 15, 24 -- Subject: raison d' etre
} 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500
} 15, 24 -- Message-ID: {000101c5944a$d5416290$dc9bd280-at-paklabpgrover}
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}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
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9, 18 -- Date: Fri, 29 Jul 2005 11:45:25 -0400
9, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
9, 18 -- Subject: Re: [Microscopy] raison d' etre
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 10:50:55 -0500
Subject: [Microscopy] Re: Fw: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

well to be honest, most of us have a life after work,
and after 25 years of 12 hour days it gets a little
old.
you know just upkeeping our lives can be a full time
job, not trying to kick anything up either.
which is one of the reasons i retired early.

--- frank.karl-at-degussa.com wrote:

}
}
}
}
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} The Microscopy ListServer -- CoSponsor: The
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}
}
}
}
}
} I find myself perplexed by the reason for Paul
} Gover’s note. I typically
} check titles and skim the content of the note if
} I’m interested.
} Everything else gets trashed canned, so I haven’t
} been paying too much
} attention to the discussion. If my lurking finds a
} theme I’m interested
} in, I’m not above kicking the embers to bring
} forth light and heat. With
} this in mind….
}
}
} I’ve been making a reasonable living as a
} microscopist for over 25 years
} and I still consider myself a very fortunate
} amateur. I too like to look
} at little things and to that end built a home
} microscopy lab while I was in
} college. I consider the time spent studying
} diatoms, collecting pollen or
} cutting free hand thin section of multi-layer
} bottles to be golden. I find
} that these skills are transferable to the
} professional arena.
}
} My first position in a multi-used environment was in
} the tire industry.
} Conversations over coffee revealed none of my new
} co-workers owned a home
} lab. My microscopy culture tells me all
} microscopists have a home lab. I
} knew, at that moment, my co-workers were simply
} employees and nothing more.
} All of the microscopist I admire have a home lab and
} several have created
} an employment and provided for the welfare of their
} family from it. Some
} find their professional lives do not provide the
} luxury of using the home
} lab and most regret it.
}
} If amateurs are not welcome on this list, I suggest
} a name change. I have
} found this server a useful tool, but I’ll hate to
} see it restricted to only
} “profession†microscopist.
}
} Frank Karl
} Degussa Corporation
}
}
} .
} ----- Forwarded by Frank Karl/AKR/Degussa-Huels/US
} on 07/29/2005 11:34 AM
} -----
}
}
}
} pgrover-at-bilbo.bio
}
}
} .purdue.edu To:
} frank.karl-at-degussa.com
}
} cc:
}
}
} 07/29/2005 10:37
} Subject: [Microscopy] raison d' etre
}
} AM
}
}
} Please respond to
}
}
} microscopy
}
}
}
}
}
}
}
}
}
}
}
}
}
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}
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} Microscopy Society of America
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}
}
} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll
} waste a little
} bandwidth and a few KB to vent. I'd like to note
} that:
}
} (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one
} must be using the latest
} multi-probe cutting edge technology, run a
} multi-user facility, or have a
} purchasing department, federal grant, or even a job.
} Our common bond is
} that we like to "look at little things".
}
} (3) The greatest scientists have been, mostly,
} AMATEURS (from Latin
} 'amator', i.e. someone who does something out of
} love instead of for
} financial gain). As we celebrate the centenary of
} Einstein's 'miracle
} year'
} which changed our concept of the universe, let's
} remember that he did this
} work as an amateur.
}
} (4) In a few years we'll all be toothless
} curmudgeons and today's
} schoolkids will be deciding whether Medicare covers
} immersion oil and cover
} slips.
}
} (5) I like to "look at little things" but I'm a
} carpenter by trade. If
} you
} don't like that, bite me.
}
} (6) If you aren't reading this it's because you're
} in Hawaii and I hate
} you
} because I'm not.
}
}
} Paul Grover :0)
}
}
}
------------------------------------------------------------------------
} No matter how much cats fight, there always seem to
} be plenty of kittens.
} - A.
} Lincoln
}
}
}
}
}
}
} ==============================Original
} Headers==============================
} 38, 19 -- From frank.karl-at-degussa.com Fri Jul 29
} 10:38:10 2005
} 38, 19 -- Received: from framailout1.rz.itson.com
} (mailout2.degussa.com [149.216.91.173])
} 38, 19 -- by ns.microscopy.com (8.12.11/8.12.8)
} with ESMTP id j6TFc49t000367
} 38, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri,
} 29 Jul 2005 10:38:10 -0500
} 38, 19 -- Received: from
} mobuscomm01.mail.degussa.com ([172.20.6.74])
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} (8.13.3/8.13.3/Debian-6) with ESMTP id
} j6TFapLj006130
} 38, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri,
} 29 Jul 2005 17:37:17 +0200
} 38, 19 -- Subject: Fw: [Microscopy] raison d' etre
} 38, 19 -- To: microscopy-at-msa.microscopy.com
} 38, 19 -- X-Mailer: Lotus Notes Release 6.5.2 June
} 01, 2004
} 38, 19 -- Message-ID:
}
{OFA5E2B331.F5B85357-ON8525704D.005583B8-8525704D.0055C455-at-degussa.com}
}
=== message truncated ===


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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Fri Jul 29 10:50:55 2005
5, 19 -- Received: from web50203.mail.yahoo.com (web50203.mail.yahoo.com [206.190.38.44])
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5, 19 -- Date: Fri, 29 Jul 2005 08:50:54 -0700 (PDT)
5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] Fw: raison d' etre
5, 19 -- To: microscopy-at-microscopy.com
5, 19 -- In-Reply-To: {200507291539.j6TFdf7p005133-at-ns.microscopy.com}
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 11:07:34 -0500
Subject: [Microscopy] Re: AskAMicroscopist: an affordable student

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

you do have a sense of humor after all, i was begining
to wonder. what a nice sardonic reply.
finlly get it i do have a name huh?

--- r.sims-at-auckland.ac.nz wrote:

}
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}
} Well said, John!
}
} I'm sure that the enquiring high school student will
} find the information you
} have so thoughtfully and kindly supplied to him to
} be useful, will encourage his
} interest in microscopy, and will induce in him a new
} respect for microscopists.
}
} Marcello, please wait a little longer and you may
} find that other list members
} may post for you information even more relevant and
} useful than John's kind reply.
}
} I would try, but what I know about light microscopy
} could easily be written on a
} small Post-It note.
}
} cheers
}
} Ritchie Sims
} Auckland
} New Zealand
}
}
} Quoting hoffpajo-at-yahoo.com:
}
} }
} }
} }
} }
}
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} }
} } have you gone through your purchasing dept?
} }
} } --- ballardmark-at-gmail.com wrote:
} }
} } }
} } }
} } }
} } }
} }
}
----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
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} } }
} } } Below is the result of your feedback form
} } } (NJZFM-ultra-55). It was
} } } submitted by (ballardmark-at-gmail.com) from
} } }
} }
}
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
} } }
} } } on Thursday, July 28, 2005 at 10:58:06
} } }
} }
}
---------------------------------------------------------------------------
} } }
} } } Email: ballardmark-at-gmail.com
} } } Name: Marcello
} } }
} } } Organization: None
} } }
} } } Education: 9-12th Grade High School
} } }
} } } Location: Orlando, Florida
} } }
} } } Question: Hi to all,
} } }
} } } i am trying to buy the most afforable
} microscopy,
} } } but that will be
} } } able to help me with biology and chemistry.
} } }
} } }
}
}
}
} -------------------------------------------------
} This mail sent through University of Auckland
} http://www.auckland.ac.nz/
}
} ==============================Original
} Headers==============================
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} 00:20:20 2005
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} 13, 35 -- Date: Fri, 29 Jul 2005 17:20:17 +1200
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} 13, 35 -- To: microscopy-at-microscopy.com
} 13, 35 -- Subject: Re: [Microscopy] Re:
} AskAMicroscopist: an affordable student
} 13, 35 -- microscope
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==============================Original Headers==============================
6, 19 -- From hoffpajo-at-yahoo.com Fri Jul 29 11:07:34 2005
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6, 19 -- Subject: Re: [Microscopy] AskAMicroscopist: an affordable student
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 11:13:51 -0500
Subject: [Microscopy] Re: snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

wasn't there an article or something in the
Smithsonian a little while back? i will have to check
my back issues.


--- pgrover-at-bilbo.bio.purdue.edu wrote:

}
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}
} And be sure to check out Wilson A. Bentley's life &
} work. He pioneered
} snowflake photography a long time ago, and did it
} the hard way. Amateurs
} rock!
}
} Paul Grover
}
}
------------------------------------------------------------------------
} No matter how much cats fight, there always seem to
} be plenty of kittens.
} - A.
} Lincoln
}
}
}
} ==============================Original
} Headers==============================
} 5, 23 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul
} 29 08:48:35 2005
} 5, 23 -- Received: from mailhub130.itcs.purdue.edu
} (mailhub130.itcs.purdue.edu [128.210.5.130])
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} ESMTP id j6TDmZu1021697
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} Jul 2005 08:48:35 -0500
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} (dhcp155-220.bio.purdue.edu [128.210.155.220])
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} Jul 2005 08:48:35 -0500
} 5, 23 -- From: "pgrover"
} {pgrover-at-bilbo.bio.purdue.edu}
} 5, 23 -- To: {microscopy-at-microscopy.com}
} 5, 23 -- Subject: snowflakes
} 5, 23 -- Date: Fri, 29 Jul 2005 08:48:35 -0500
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}




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==============================Original Headers==============================
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7, 19 -- Subject: Re: [Microscopy] snowflakes
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From: cammer-at-aecom.yu.edu
Date: Fri, 29 Jul 2005 11:32:11 -0500
Subject: [Microscopy] Re: snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

more "amateur" snowflake pics, 6 web pages beginning at
http://cammer.net/blog/snowflake.htm and ending with a movie at
http://cammer.net/blog/snowflake06.htm

Also a favorite at
http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm





At 08:49 AM 07/29/05 -0500, you wrote:



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____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**


==============================Original Headers==============================
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From: Elliott-at-arizona.edu
Date: Fri, 29 Jul 2005 11:44:03 -0500
Subject: [Microscopy] snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How about

http://emu.arsusda.gov/snowsite/default.html


David


On Jul 29, 2005, at 9:34 AM, cammer-at-aecom.yu.edu wrote:

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}
} more "amateur" snowflake pics, 6 web pages beginning at
} http://cammer.net/blog/snowflake.htm and ending with a movie at
} http://cammer.net/blog/snowflake06.htm
}
} Also a favorite at
} http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm
}
}
}
}
}
} At 08:49 AM 07/29/05 -0500, you wrote:
}
}
}
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
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} } ----------------------------------------------------------------------
} } ------
} }
} } And be sure to check out Wilson A. Bentley's life & work. He
} } pioneered
} } snowflake photography a long time ago, and did it the hard way.
} } Amateurs
} } rock!
} }
} } Paul Grover
} }
} } ----------------------------------------------------------------------
} } --
} } No matter how much cats fight, there always seem to be plenty of
} } kittens.
} } - A. Lincoln
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 5, 23 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 08:48:35 2005
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}
} _______________________________________________________________________
} _____
} Michael Cammer Analytical Imaging Facility Albert Einstein Coll.
} of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY
} 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
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} ==============================Original
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From: tivol-at-caltech.edu
Date: Fri, 29 Jul 2005 12:13:31 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow flake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jul 28, 2005, at 7:06 PM, ferretinmicrowave-at-ns.microscopy.com wrote:

} Question: How do you take an image of a snowflake with an optical
} microscope? What sort of special techniques and equipment do you
} need in order to take the picture?
}
Dear Isabel,
There is a wonderful book by Dr. Ken Libbrecht called The Snowflake,
which has some information about how the incredible images in the book
were taken. Basically, he took a microscope slide to collect the
flakes as they fell and kept everything cold while providing
appropriate illumination for microphotography. If you need details not
provided in the book, I'm pretty sure that Ken would be willing to help
you out. I can give you his contact info off-list if you want.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: msimms-at-tracelabs.com
Date: Fri, 29 Jul 2005 12:49:28 -0500
Subject: [Microscopy] viaWWW: Measurement of ink depth/contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (msimms-at-tracelabs.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, July 29, 2005 at 10:06:10
---------------------------------------------------------------------------

Email: msimms-at-tracelabs.com
Name: Michael Simms

Organization: Trace Laboratories - Central

Title-Subject: [Filtered] Measurement of ink depth/contrast

Question: Hello Gentlemen,
I work for an independent testing organization, Trace Laboratories - Central.
We have been asked to have printing ink measured for depth and
contrast. Laser printing is done on the insulation sleeve of a cable.
per Sikorsky specification SS7333, Paragraph 4.6.5
This asks for measuring to an accuracy of 0.0001 inch with a contour
projector or calibrated microscope or Zygo measuring device. The
contrast measurement is suggested to be made with a Spectrum
Technology CMS^2 Contrast Measuring System. I would need evidence of
either an accredited laboratory or calibration to pass on to the
customer.

Is this something that someone associated with this resource might be
able to quote?

Regards,
Mike

Mike Simms
Chemist
Trace Laboratories - Central
1150 W. Euclid Ave.
Palatine, IL 60067

phone 847-934-5300
fax 847-934-4600
www.tracelabs.com



---------------------------------------------------------------------------

==============================Original Headers==============================
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12, 12 -- Subject: viaWWW: Measurement of ink depth/contrast
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From: aetmicro-at-optonline.net
Date: Fri, 29 Jul 2005 15:10:04 -0500
Subject: [Microscopy] viaWWW: unstable beam EM300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (aetmicro-at-optonline.net) from
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
Friday, July 29, 2005 at 13:07:28
---------------------------------------------------------------------------

Email: aetmicro-at-optonline.net
Name: andrew thelian

Organization: nanoprobes, inc

Title-Subject: [Filtered] MListserver: unstable beam

Question: Hi,

I have been having a problem with my beam stability currently... I
operate a Philips EM300... and in an attempt to regain stability i
cleaned the contacts of the high tension line that runs from the gun
to the high voltage tank... (the cleaning included the insulators as
well)

My questions are... What kind of oil do I use inside the insulators?
(i was advised to use Santovac 5 and would like verification because
of its cost) What kind of oil should I use for the High Voltage Tank?

Any links to locate vendors would be of great help.

Thank You,
Andy

---------------------------------------------------------------------------

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From: derby-at-nmt.edu
Date: Fri, 29 Jul 2005 17:00:34 -0500
Subject: [Microscopy] Re: viaWWW: unstable beam EM300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

on 7/29/05 2:14 PM, aetmicro-at-optonline.net at aetmicro-at-optonline.net wrote:

} Santovac 5

The Santovac oil is for the diffusion pump *NOT* the high tension tank.
I use to know that info. but it has gone poof.
I would call FEI they took over Philips or the changed their name they would
know.

Robert Derby
New Mexico Tech.



==============================Original Headers==============================
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6, 17 -- Subject: Re: [Microscopy] viaWWW: unstable beam EM300
6, 17 -- From: derby {derby-at-nmt.edu}
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From: aetmicro-at-optonline.net
Date: Sat, 30 Jul 2005 07:15:00 -0500
Subject: [Microscopy] viaWWW: unstable beam EM300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I read that as well... being that Santovac 5 is made for diffusion pumps...
I wasn't going to use it for the high voltage tank... but for the high
tension line insulators from the gun to the tank...

I believe its extremely low water content is what makes it a good choice for
the insulators... to prevent arcing...

Thank you for your help I am very appreciative:),
Andrew

--
No virus found in this outgoing message.
Checked by AVG Anti-Virus.
Version: 7.0.338 / Virus Database: 267.9.5/58 - Release Date: 7/25/2005



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From: kgmnrc-at-hotmail.com
Date: Sat, 30 Jul 2005 07:51:15 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Bill:
A very complete theses on how to do it and how it was done please see
Historical Microsopical Society of Canada Bulletin Volume 14 No 55 February
2003 Most of the paper dealt with snow crystals / snow flakes. Alas the
Historical Microsopical Society of Canada is no more it is part of the
Microscope Historical Society.
Hope this helps.
Regards,
Keith

-----Original Message-----
X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
Sent: Friday, July 29, 2005 1:17 PM
To: kgmnrc-at-hotmail.com


On Jul 28, 2005, at 7:06 PM, ferretinmicrowave-at-ns.microscopy.com wrote:

} Question: How do you take an image of a snowflake with an optical
} microscope? What sort of special techniques and equipment do you
} need in order to take the picture?
}
Dear Isabel,
There is a wonderful book by Dr. Ken Libbrecht called The Snowflake,

which has some information about how the incredible images in the book
were taken. Basically, he took a microscope slide to collect the
flakes as they fell and kept everything cold while providing
appropriate illumination for microphotography. If you need details not
provided in the book, I'm pretty sure that Ken would be willing to help
you out. I can give you his contact info off-list if you want.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



==============================Original Headers==============================
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5, 27 -- From: Bill Tivol {tivol-at-caltech.edu}
5, 27 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: How to image
a snow flake
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From: r.sims-at-auckland.ac.nz
Date: Sat, 30 Jul 2005 15:46:43 -0500
Subject: [Microscopy] Santovac for insulation

Contents Retrieved from Microscopy Listserver Archives
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I suspect that transformer oil might do a better job as well as costing far less.

But FEI will have good advice on this, anyway.

cheers

rtch

}
} Hi,
}
} I read that as well... being that Santovac 5 is made for diffusion
} pumps... I wasn't going to use it for the high voltage tank... but for
} the high tension line insulators from the gun to the tank...
}
} I believe its extremely low water content is what makes it a good
} choice for the insulators... to prevent arcing...
}
} Thank you for your help I am very appreciative:),
} Andrew
}


--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


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From: stefan.diller-at-t-online.de
Date: Sat, 30 Jul 2005 18:02:47 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Reichert Histostat manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (stefan.diller-at-t-online.de) from
http://microscopy.com/MLFormMail.html on Saturday, July 30, 2005 at
13:43:48
---------------------------------------------------------------------------

Email: stefan.diller-at-t-online.de
Name: Stefan Diller

Title-Subject: [Filtered] Reichert Histostat 8035 Paraffin Embedder
manual needed

Question: Dear members,
I am looking urgently for a user manual and if possible a service
manual or the electronic layout for the Reichert Histostat Paraffin
Embedder Modell 8035.

Best regards,
Stefan Diller


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From: hoffpajo-at-yahoo.com
Date: Sat, 30 Jul 2005 19:13:31 -0500
Subject: [Microscopy] Re: viaWWW: unstable beam EM300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

it's probly been over 15 years since i used a 300. but
you might want to contact FEI at www.feic.com. if you
contact me off line i can give you an email address of
someone to talk to directly at FEI.
john

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} Below is the result of your feedback form
} (NJZFM-ultra-55). It was
} submitted by (aetmicro-at-optonline.net) from
}
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} on
} Friday, July 29, 2005 at 13:07:28
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} Email: aetmicro-at-optonline.net
} Name: andrew thelian
}
} Organization: nanoprobes, inc
}
} Title-Subject: [Filtered] MListserver: unstable beam
}
} Question: Hi,
}
} I have been having a problem with my beam stability
} currently... I
} operate a Philips EM300... and in an attempt to
} regain stability i
} cleaned the contacts of the high tension line that
} runs from the gun
} to the high voltage tank... (the cleaning included
} the insulators as
} well)
}
} My questions are... What kind of oil do I use inside
} the insulators?
} (i was advised to use Santovac 5 and would like
} verification because
} of its cost) What kind of oil should I use for the
} High Voltage Tank?
}
} Any links to locate vendors would be of great help.
}
} Thank You,
} Andy
}
}
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}
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} Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Sat, 30 Jul 2005 19:20:35 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow flake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

here is a big a list of web sites as i can compile
Snow from eduScapes 42eXplore
http://eduscapes.com/42explore/snow.htm
Make Snowflakes from KinderArt
http://www.kinderart.com/seasons/dec7.shtml
Snow
http://www.muohio.edu/dragonfly/snow/snow.HTMLX
Snow facts
http://tqjunior.advanced.org/3876/snowfacts.html
Old Camera Photos
http://www.flash.net/~bobgil/cam/camera.html
Make Your Own Virtual Snowflake
http://www.muohio.edu/dragonfly/snow/icensnow.htmlx
Edible Snowflakes Recipe
http://www.stepbystepcc.com/holidays/christmas5.html
Scientists See Snowflakes
http://www.ars.usda.gov/is/kids/environment/story2/snowflakeframes.htm

Snowflakes information
http://www.macatawa.org/~oias/snowflak.htm
Using a Microscope for Snowflakes
http://www.microscopy-uk.org.uk/mag/artfeb00/eksnow.html

Wilson A. Bentley --The Snowflake Man
http://www.snowflakebentley.com/
Pictures of His Snowflakes
http://www.snowflakebentley.com/snowflakes.htm
Life of Wilson Bentley (middle grades)
http://www.virtualvermont.com/history/sbentley.html
Jericho, Vermont
http://www.jericho-underhill.com/
Famous People from Vermont
http://www.virtualvermont.com/history/people.html
Photography from eduScapes 42eXplore
http://eduscapes.com/42explore/photog.htm

Educator Links
Story of Wilson Bentley Life (Upper level reading)
http://www.snowflakebentley.com/sfman.htm
http://www.snowflakebentley.com/prior.html
Photographing Snowflakes (Upper level reading)
http://www.snowflakebentley.com/wbsf.htm
Snowflakes- A Thematic Approach
http://www.wsanford.com/~wsanford/exo/n-m_snowflakes.html

Further Reading About Ice and Snow
http://www.muohio.edu/dragonfly/snow/readings.HTMLX
Making a Snowflake (Upper level reading)
http://highhopes.com/snowflakes.html
History of Photography (Mature/adult)
http://home.globalcrossing.net/~sos/history.html
History of Photography (Mature/adult)
http://www.rleggat.com/photohistory/
Snowflake Bentley
http://www.carolhurst.com/titles/snowflakebentley.html

Suddenly Snow--Snowflake Activities
http://www.earthwalk.com/techwize/volume1/january/JANLESSON.HTML




--- kgmnrc-at-hotmail.com wrote:

}
}
}
}
----------------------------------------------------------------------------
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}
} Dear Bill:
} A very complete theses on how to do it and how it
} was done please see
} Historical Microsopical Society of Canada Bulletin
} Volume 14 No 55 February
} 2003 Most of the paper dealt with snow crystals /
} snow flakes. Alas the
} Historical Microsopical Society of Canada is no more
} it is part of the
} Microscope Historical Society.
} Hope this helps.
} Regards,
} Keith
}
} -----Original Message-----
} X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
}
} Sent: Friday, July 29, 2005 1:17 PM
} To: kgmnrc-at-hotmail.com
} Subject: [Microscopy] Re: [Filtered]
} AskAMicroscopist: How to image a snow
} flake
}
}
}
}
}
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}
}
} On Jul 28, 2005, at 7:06 PM,
} ferretinmicrowave-at-ns.microscopy.com wrote:
}
} } Question: How do you take an image of a snowflake
} with an optical
} } microscope? What sort of special techniques and
} equipment do you
} } need in order to take the picture?
} }
} Dear Isabel,
} There is a wonderful book by Dr. Ken Libbrecht
} called The Snowflake,
}
} which has some information about how the incredible
} images in the book
} were taken. Basically, he took a microscope slide
} to collect the
} flakes as they fell and kept everything cold while
} providing
} appropriate illumination for microphotography. If
} you need details not
} provided in the book, I'm pretty sure that Ken would
} be willing to help
} you out. I can give you his contact info off-list
} if you want.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
} ==============================Original
} Headers==============================
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} a snow flake
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From: frah0010-at-tc.umn.edu
Date: Sat, 30 Jul 2005 21:21:50 -0500
Subject: [Microscopy] question: JEOL 8900 control

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Microscopy folks,

First, apologies for cross-posting with the Microbeam Analysis
Society...

Second, apologies for sending everyone a question with such a narrow
focus...

I manage the University of Minnesota's Electron Microprobe Lab, and
we have a JEOL JXA-8900R. I am looking to replace our original 1994-
vintage HP workstation with something newer and more reliable. I am
investigating our options, including just a new HP workstation and
switching to the "Probe for Windows" software. I know that my
supervisor and the department head will ask if I have checked into
every option. In particular, I anticipate they will ask me about the
possibility of running the JEOL software on a PC running Linux rather
than a HP workstation running HP-UX. Has anyone ever tried this?
Would it work? Wouldn't it? I suspect that it wouldn't fully
function, but I'd like to be able to speak to the issue with more
information than just my guess. Any information or opinions are most
welcome.

Thanks,
Ellery

--------------------
Ellery E. Frahm
Research Scientist/Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu


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7, 15 -- From: Ellery Frahm {frah0010-at-tc.umn.edu}
7, 15 -- Subject: question: JEOL 8900 control
7, 15 -- Date: Sat, 30 Jul 2005 21:21:48 -0500
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