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Hi Andy

To save writing out all the options please take a look at our web site HINTS
and TIPS to check where your problem is,
is it the gun, gun chamber, cable or high voltage tank? Do not consider
that the only problem is likely to be the insulation of the gun. Chamber
cleanliness, vacuum level etc all play a part in high voltage breakdown a
feature that will result in beam instability.

But is it beam instability due to the gun (emission meter variations,
virtual source fluttering) or due to contamination in the condenser system
illumination flutter or movement)?

Hope this helps?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel +44 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com

----- Original Message -----
X-from: {aetmicro-at-optonline.net}
To: {protrain-at-emcourses.com}
Sent: Friday, July 29, 2005 9:13 PM

Dear Members
I am trying to accumulate literature sources that explain how best
to prepare insect specimens for sectioning so that the medium adheres well
and such that the knife doesn't 'catch' the specimen and 'drag' it out of
the medium. I would like to start with semi-thin sections of wings, and
then ultra thin for TEM. If there are any 'inside' tricjs that are
unpublished or otherwise unlikely to be encountered, I would be grateful.
Thanks
Ian

Ian Stocks
308 Long Hall
Clemson University
864 656 5058
istocks-at-clemson.edu

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3, 19 -- Subject: LM- embedding insect cuticle
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Post-Doctoral Research Associate

A post-doctoral research associate position is available in the Center
for Solid State Science at Arizona State University. The research
project involves in situ synthesis and characterization of carbon
nanotubes using a state of the art Environmental Transmission Electron
Microscope, Tecnai F20-ETEM/STEM. Applicants must have a Ph.D in
Chemistry, Physics or Materials Science with experience in
high-resolution imaging, electron energy-loss spectroscopy using
transmission electron microscope. Experience in the areas of scanning
transmission electron microscopy, structural and/or theoretical modeling
is desired. The successful candidate will have an advantage of working
with the cutting edge technology and in a highly motivating environment.

The position is for one year with a start date in August. Deadline is
August 15, 2005, if not filled, weekly thereafter until search closed.
Salary is $34,000/year. Interested candidates must send their resume,
list of publications and the name, address, and phone number of three
references to: Dr Renu Sharma, Center for Solid State Science, Arizona
State University, Tempe, AZ 85287-1704. Email renu.sharma-at-asu.edu.

==============================Original Headers==============================
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Dear Ian,
For LM use London Resin and try to match the hardness of the resin to
the tissue eg use the hardest grade for highly sclerotised beetle
cuticle and the medium grade for most cuticles. Tricks like orienting
the block so the knife cuts the cuticle first then cuts through the
underlying tissue will help but the hydrophobic epicuticle of some
insects will never bond to the resin when using standard
glutaraldehyde/osmium fixations. Yell if you want more detail.
Cheers,
Eric Hines
CSIRO Entomology
Canberra OZ

} -----Original Message-----
} From: istocks-at-CLEMSON.EDU [mailto:istocks-at-CLEMSON.EDU]
} Sent: Monday, 1 August 2005 11:26 PM
} To: Hines, Eric (Entomology, Black Mountain)
} Subject: [Microscopy] LM- embedding insect cuticle
}
}
}
}
}
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}
} Dear Members
} I am trying to accumulate literature sources that
} explain how best
} to prepare insect specimens for sectioning so that the medium
} adheres well
} and such that the knife doesn't 'catch' the specimen and
} 'drag' it out of
} the medium. I would like to start with semi-thin sections of
} wings, and
} then ultra thin for TEM. If there are any 'inside' tricjs that are
} unpublished or otherwise unlikely to be encountered, I would
} be grateful. Thanks Ian
}
} Ian Stocks
} 308 Long Hall
} Clemson University
} 864 656 5058
} istocks-at-clemson.edu
}
}
} ==============================Original
} Headers==============================
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3, 29 -- From Eric.Hines-at-csiro.au Mon Aug 1 18:56:56 2005
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.

--- cammer-at-aecom.yu.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
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}
} more "amateur" snowflake pics, 6 web pages beginning
} at
} http://cammer.net/blog/snowflake.htm and ending with
} a movie at
} http://cammer.net/blog/snowflake06.htm
}
} Also a favorite at
}
http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm
}
}
}
}
}
} At 08:49 AM 07/29/05 -0500, you wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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}
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} ----------------------------------------------------------------------------
} }
} } And be sure to check out Wilson A. Bentley's life &
} work. He pioneered
} } snowflake photography a long time ago, and did it
} the hard way. Amateurs
} } rock!
} }
} } Paul Grover
} }
}
} ------------------------------------------------------------------------
} } No matter how much cats fight, there always seem to
} be plenty of kittens.
} } -
} A. Lincoln
} }
} }
} }
} } ==============================Original
} Headers==============================
} } 5, 23 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul
} 29 08:48:35 2005
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} {pgrover-at-bilbo.bio.purdue.edu}
} } 5, 23 -- To: {microscopy-at-microscopy.com}
} } 5, 23 -- Subject: snowflakes
} } 5, 23 -- Date: Fri, 29 Jul 2005 08:48:35 -0500
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}
}
____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility
} Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park
} Ave. Bronx, NY 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains
} information that is privileged.**
}
}
} ==============================Original
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} 11:32:11 2005
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} 12, 23 -- Subject: Re: [Microscopy] snowflakes
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__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

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First the flame: why assume we only like looking at little itty bitty
things?

On to real comments: Actually, I like looking at anything (size doesn't
count) and I like making cool and/or pretty pictures and I like math puzzles.

Being a microscopist fulfills all of these and pays my bills. To support
my job and my interests, to have access to the cool materials and
technologies that keep me fascinated, I need to be concerned with the
latest multi-probe cutting edge technologies, running a multi-user
facility, having a
purchasing department, federal grants, personnel and a job. Such is reality.

The conclusion is:
We should be discussing both the microscopies (cool pictures and how to
make them regardless whether they are snowflakes for the elementary
classroom or biological processes to understand cutting edge protein
interactions) and the processes for supporting the microscopies (e.g.
federal grants vs. commercial).

I've been subscribed here for years. It seems to me this listserv is doing
exactly what I describe/prescribe. Do we really need to discuss this more?

-Michael

} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll waste a little
} bandwidth and a few KB to vent. I'd like to note that:
}
} (1) This is a MICROSCOPY listserver. This apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one must be using the latest
} multi-probe cutting edge technology, run a multi-user facility, or have a
} purchasing department, federal grant, or even a job. Our common bond is
} that we like to "look at little things".

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**

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9, 23 -- Subject: Re: [Microscopy] raison d' etre
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Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey
with vapors of osmium over the years. Although we are
taking every precautions to avoid leakage of osmium
vapor from its container, this is a problem that I have
observed in every EM lab that I have worked in. Does
someone on the list have a method to store osmium in such
a way as to avoid any escape of vapor? Also, is there
something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

==============================Original Headers==============================
5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48])
5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j72G9BnV011512
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5, 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005 12:09:29 -0400 (EDT)
5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
5, 18 -- Subject: Osmium vapors
5, 18 -- In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
5, 18 -- To: microscopy-at-microscopy.com
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he was just kidding around you know.

--- cammer-at-aecom.yu.edu wrote:

}
}
}
}
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}
} First the flame: why assume we only like looking at
} little itty bitty
} things?
}
} On to real comments: Actually, I like looking at
} anything (size doesn't
} count) and I like making cool and/or pretty pictures
} and I like math puzzles.
}
} Being a microscopist fulfills all of these and pays
} my bills. To support
} my job and my interests, to have access to the cool
} materials and
} technologies that keep me fascinated, I need to be
} concerned with the
} latest multi-probe cutting edge technologies,
} running a multi-user
} facility, having a
} purchasing department, federal grants, personnel and
} a job. Such is reality.
}
} The conclusion is:
} We should be discussing both the microscopies (cool
} pictures and how to
} make them regardless whether they are snowflakes for
} the elementary
} classroom or biological processes to understand
} cutting edge protein
} interactions) and the processes for supporting the
} microscopies (e.g.
} federal grants vs. commercial).
}
} I've been subscribed here for years. It seems to me
} this listserv is doing
} exactly what I describe/prescribe. Do we really
} need to discuss this more?
}
} -Michael
}
} } Esteemed Microscopists,
} }
} } Since most of you apparently are in Hawaii now,
} I'll waste a little
} } bandwidth and a few KB to vent. I'd like to note
} that:
} }
} } (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} } little things".
} }
} } (2) There is apparently no requirement that one
} must be using the latest
} } multi-probe cutting edge technology, run a
} multi-user facility, or have a
} } purchasing department, federal grant, or even a
} job. Our common bond is
} } that we like to "look at little things".
}
}
____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility
} Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park
} Ave. Bronx, NY 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains
} information that is privileged.**
}
}
} ==============================Original
} Headers==============================
} 9, 23 -- From cammer-at-aecom.yu.edu Tue Aug 2
} 11:05:59 2005
} 9, 23 -- Received: from mailgw.aecom.yu.edu
} (mailgw.aecom.yu.edu [129.98.1.16])
} 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j72G5wXq005120
} 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2
} Aug 2005 11:05:59 -0500
} 9, 23 -- Received: from mailvx.aecom.yu.edu
} (mailvx.aecom.yu.edu [129.98.1.17])
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} with SMTP id j72G5Jb5023564
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} Aug 2005 12:05:58 -0400
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} ([129.98.1.100])
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} with SMTP id M2005080212055813185
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} Aug 2005 12:05:58 -0400
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} (aif3.aif.aecom.yu.edu [129.98.30.137])
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} Aug 2005 12:05:58 -0400 (EDT)
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}
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} 9, 23 -- Date: Tue, 02 Aug 2005 12:05:58 -0400
} 9, 23 -- To: microscopy-at-microscopy.com
} 9, 23 -- From: Michael Cammer {cammer-at-aecom.yu.edu}
} 9, 23 -- Subject: Re: [Microscopy] raison d' etre
} 9, 23 -- In-Reply-To:
} {200507291437.j6TEboiR011778-at-ns.microscopy.com}
} 9, 23 -- Mime-Version: 1.0
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} charset="us-ascii"; format=flowed
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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:11:28 2005
5, 19 -- Received: from web50204.mail.yahoo.com (web50204.mail.yahoo.com [206.190.38.45])
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5, 19 -- Date: Tue, 2 Aug 2005 09:11:26 -0700 (PDT)
5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] Re: raison d' etre
5, 19 -- To: microscopy-at-microscopy.com
5, 19 -- In-Reply-To: {200508021607.j72G7KJW007670-at-ns.microscopy.com}
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you could try placing the bottle in a bath of oil, but
that would get messy.

--- marc.pypaert-at-yale.edu wrote:

}
}
}
}
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} Microscopy Society of America
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}
} Question to the list:
}
} Our local safety inspector is concerned about the
} appearance
} of the fridge where we stock our solution of osmium.
} As you
} can expect the white interior of the fridge has
} turned grey
} with vapors of osmium over the years. Although we
} are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I
} have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in
} such
} a way as to avoid any escape of vapor? Also, is
} there
} something we could put into fridge that would trap
} osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original
} Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} 11:09:11 2005
} 5, 18 -- Received: from BIOMED.MED.YALE.EDU
} (biomed.med.yale.edu [130.132.232.48])
} 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j72G9BnV011512
} 5, 18 -- for {microscopy-at-microscopy.com} ; Tue, 2
} Aug 2005 11:09:11 -0500
} 5, 18 -- Received: from yale.edu
} (net234-111.med.yale.edu [130.132.234.111])
} 5, 18 -- by biomed.med.yale.edu (PMDF V6.1-1
} #30532)
} 5, 18 -- with ESMTP id
} {01LRCOV1R88000DGI6-at-biomed.med.yale.edu} for
} 5, 18 -- microscopy-at-microscopy.com; Tue, 02 Aug
} 2005 12:09:29 -0400 (EDT)
} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
} 5, 18 -- In-reply-to:
} {200508021530.j72FU83w031285-at-ns.microscopy.com}
} 5, 18 -- To: microscopy-at-microscopy.com
} 5, 18 -- Message-id:
} {C13CE8F5-036F-11DA-AC3D-0030659833B4-at-yale.edu}
} 5, 18 -- MIME-version: 1.0 (Apple Message framework
} v553)
} 5, 18 -- X-Mailer: Apple Mail (2.553)
} 5, 18 -- Content-type: text/plain; format=flowed;
} charset=US-ASCII
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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:13:12 2005
5, 19 -- Received: from web50209.mail.yahoo.com (web50209.mail.yahoo.com [206.190.38.50])
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5, 19 -- Date: Tue, 2 Aug 2005 09:13:11 -0700 (PDT)
5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] Osmium vapors
5, 19 -- To: microscopy-at-microscopy.com
5, 19 -- In-Reply-To: {200508021610.j72GAeX8015207-at-ns.microscopy.com}
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i was trying to send that directly back to poster. my
bad i guess.

--- hoffpajo-at-yahoo.com wrote:

}
}
}
}
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}
} he was just kidding around you know.
}
} --- cammer-at-aecom.yu.edu wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
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} }
}
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} }
}
----------------------------------------------------------------------------
} }
} } First the flame: why assume we only like looking
} at
} } little itty bitty
} } things?
} }
} } On to real comments: Actually, I like looking at
} } anything (size doesn't
} } count) and I like making cool and/or pretty
} pictures
} } and I like math puzzles.
} }
} } Being a microscopist fulfills all of these and
} pays
} } my bills. To support
} } my job and my interests, to have access to the
} cool
} } materials and
} } technologies that keep me fascinated, I need to be
} } concerned with the
} } latest multi-probe cutting edge technologies,
} } running a multi-user
} } facility, having a
} } purchasing department, federal grants, personnel
} and
} } a job. Such is reality.
} }
} } The conclusion is:
} } We should be discussing both the microscopies
} (cool
} } pictures and how to
} } make them regardless whether they are snowflakes
} for
} } the elementary
} } classroom or biological processes to understand
} } cutting edge protein
} } interactions) and the processes for supporting the
} } microscopies (e.g.
} } federal grants vs. commercial).
} }
} } I've been subscribed here for years. It seems to
} me
} } this listserv is doing
} } exactly what I describe/prescribe. Do we really
} } need to discuss this more?
} }
} } -Michael
} }
} } } Esteemed Microscopists,
} } }
} } } Since most of you apparently are in Hawaii now,
} } I'll waste a little
} } } bandwidth and a few KB to vent. I'd like to note
} } that:
} } }
} } } (1) This is a MICROSCOPY listserver. This
} } apparently means "looking at
} } } little things".
} } }
} } } (2) There is apparently no requirement that one
} } must be using the latest
} } } multi-probe cutting edge technology, run a
} } multi-user facility, or have a
} } } purchasing department, federal grant, or even a
} } job. Our common bond is
} } } that we like to "look at little things".
} }
} }
}
____________________________________________________________________________
} } Michael Cammer Analytical Imaging Facility
} } Albert Einstein Coll. of Med.
} } Jack & Pearl Resnick Campus 1300 Morris Park
} } Ave. Bronx, NY 10461
} } (718) 430-2890 Fax: 430-8996 URL:
} } http://www.aecom.yu.edu/aif/
} } **This electronic transmission contains
} } information that is privileged.**
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 23 -- From cammer-at-aecom.yu.edu Tue Aug 2
} } 11:05:59 2005
} } 9, 23 -- Received: from mailgw.aecom.yu.edu
} } (mailgw.aecom.yu.edu [129.98.1.16])
} } 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id j72G5wXq005120
} } 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2
} } Aug 2005 11:05:59 -0500
} } 9, 23 -- Received: from mailvx.aecom.yu.edu
} } (mailvx.aecom.yu.edu [129.98.1.17])
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} } with SMTP id j72G5Jb5023564
} } 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2
} } Aug 2005 12:05:58 -0400
} } 9, 23 -- Received: from post.aecom.yu.edu
} } ([129.98.1.100])
} } 9, 23 -- by mailvx.aecom.yu.edu (SAVSMTP
} 3.1.1.32)
} } with SMTP id M2005080212055813185
} } 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 02
} } Aug 2005 12:05:58 -0400
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} } (aif3.aif.aecom.yu.edu [129.98.30.137])
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} ESMTP
} } id 91E0B2FC6
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} } Aug 2005 12:05:58 -0400 (EDT)
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} }
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} } 9, 23 -- X-Mailer: QUALCOMM Windows Eudora Version
} } 5.2.1
} } 9, 23 -- Date: Tue, 02 Aug 2005 12:05:58 -0400
} } 9, 23 -- To: microscopy-at-microscopy.com
} } 9, 23 -- From: Michael Cammer
} {cammer-at-aecom.yu.edu}
} } 9, 23 -- Subject: Re: [Microscopy] raison d' etre
} } 9, 23 -- In-Reply-To:
} } {200507291437.j6TEboiR011778-at-ns.microscopy.com}
} } 9, 23 -- Mime-Version: 1.0
} } 9, 23 -- Content-Type: text/plain;
} } charset="us-ascii"; format=flowed
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} } Headers==============================
} }
}
}
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} Do You Yahoo!?
} Tired of spam? Yahoo! Mail has the best spam
} protection around
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}
} ==============================Original
} Headers==============================
} 5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:11:28
} 2005
} 5, 19 -- Received: from web50204.mail.yahoo.com
} (web50204.mail.yahoo.com [206.190.38.45])
} 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Tuesday, August 02, 2005 11:13 AM
To: msimms-at-tracelabs.com

Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey
with vapors of osmium over the years. Although we are
taking every precautions to avoid leakage of osmium
vapor from its container, this is a problem that I have
observed in every EM lab that I have worked in. Does
someone on the list have a method to store osmium in such
a way as to avoid any escape of vapor? Also, is there
something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

==============================Original Headers==============================
5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu
[130.132.232.48])
5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
j72G9BnV011512
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-0500
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5, 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005 12:09:29 -0400 (EDT)
5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
5, 18 -- Subject: Osmium vapors
5, 18 -- In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
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==============================Original Headers==============================
16, 15 -- From msimms-at-tracelabs.com Tue Aug 2 11:19:49 2005
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Marc

We store our osmium in a secondary container...a large poly pro bottle
(capped) and then we bag that container in a plastic Ziploc. It helps a
bit.

Dorrance

-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Tuesday, August 02, 2005 9:10 AM
To: McLean, Dorrance

Question to the list:

Our local safety inspector is concerned about the appearance of the
fridge where we stock our solution of osmium. As you can expect the
white interior of the fridge has turned grey with vapors of osmium over
the years. Although we are taking every precautions to avoid leakage of
osmium vapor from its container, this is a problem that I have observed
in every EM lab that I have worked in. Does someone on the list have a
method to store osmium in such a way as to avoid any escape of vapor?
Also, is there something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

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16, 29 -- From dmclea-at-sandia.gov Tue Aug 2 11:23:07 2005
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When OsO4 came in metal cans, I found by placing the working solution in a
Wheaton dropper bottle, wrap the bulb with Parafilm, place the bottle in the
can, close the lid, wrap the lid with electrician tape, and place the can in
a plastic Ziplock bag worked great. You could essentially use any clean
glass container, not just a Wheaton bottle.

After 20 years of working with OsO4, the inside of the refrigerator was
pristine. It requires diligence to unwrap and wrap the container, but it
worked.

A short-cut to the above mentioned technique, was to use a clean pint paint,
place the Wheaton bottle in the can and push the lid down tight, then place
the entire can in a Ziplock bag.

Regards,
Ken
_______________________________________
Kenneth L. Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N Willamette Blvd.
Portland, OR 97203 USA

Tel.: 503.943.8861
Email: tiekotte-at-up.edu

On 8/2/05 9:09 AM, "marc.pypaert-at-yale.edu" {marc.pypaert-at-yale.edu} wrote:

}
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}
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} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
} 5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
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9, 17 -- From tiekotte-at-up.edu Tue Aug 2 11:26:35 2005
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9, 17 -- Subject: Re: [Microscopy] Osmium vapors
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in the alternative and it may be more expensive and
you may not want to go there. and if someone with a
better memory than i do anymore, can correct me. i do
remember seeing premade OsO4 in small amounts. use
what you need, perhaps even batch the tissue samples.
then throw out the unused in the waste.
just a thought.

--- dmclea-at-sandia.gov wrote:

}
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}
} Marc
}
} We store our osmium in a secondary container...a
} large poly pro bottle
} (capped) and then we bag that container in a plastic
} Ziploc. It helps a
} bit.
}
} Dorrance
}
} -----Original Message-----
} X-from: marc.pypaert-at-yale.edu
} [mailto:marc.pypaert-at-yale.edu]
} Sent: Tuesday, August 02, 2005 9:10 AM
} To: McLean, Dorrance
} Subject: [Microscopy] Osmium vapors
}
}
}
}
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} ----
}
} Question to the list:
}
} Our local safety inspector is concerned about the
} appearance of the
} fridge where we stock our solution of osmium. As you
} can expect the
} white interior of the fridge has turned grey with
} vapors of osmium over
} the years. Although we are taking every precautions
} to avoid leakage of
} osmium vapor from its container, this is a problem
} that I have observed
} in every EM lab that I have worked in. Does someone
} on the list have a
} method to store osmium in such a way as to avoid any
} escape of vapor?
} Also, is there something we could put into fridge
} that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original
} Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} 11:09:11 2005 5, 18 --
} Received: from BIOMED.MED.YALE.EDU
} (biomed.med.yale.edu
} [130.132.232.48])
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} ESMTP id
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5, 18
} -- From: Marc
} Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} Osmium vapors 5, 18 --
} In-reply-to:
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} 2005
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____________________________________________________
Start your day with Yahoo! - make it your home page
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We use Osmium in our lab and keep it in the refrigerator.
We keep it in a glass bottle and cover the lid with
parafilm. Then we put that bottle in another bottle with a
screw top and wrap the bottle in foil. It works great, our
refrigerator doesn't have and black residue, just the two
bottles.

rebecca

==============================Original Headers==============================
2, 15 -- From gunkelrl-at-slu.edu Tue Aug 2 11:47:48 2005
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is mannie Mannie Steglich still around? haven't heard
from him in a while.
john

--- hoffpajo-at-yahoo.com wrote:

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}
} in the alternative and it may be more expensive and
} you may not want to go there. and if someone with a
} better memory than i do anymore, can correct me. i
} do
} remember seeing premade OsO4 in small amounts. use
} what you need, perhaps even batch the tissue
} samples.
} then throw out the unused in the waste.
} just a thought.
}
} --- dmclea-at-sandia.gov wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
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}
----------------------------------------------------------------------------
} }
} } Marc
} }
} } We store our osmium in a secondary container...a
} } large poly pro bottle
} } (capped) and then we bag that container in a
} plastic
} } Ziploc. It helps a
} } bit.
} }
} } Dorrance
} }
} } -----Original Message-----
} } X-from: marc.pypaert-at-yale.edu
} } [mailto:marc.pypaert-at-yale.edu]
} } Sent: Tuesday, August 02, 2005 9:10 AM
} } To: McLean, Dorrance
} } Subject: [Microscopy] Osmium vapors
} }
} }
} }
} }
} }
}
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}
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} } ----
} }
} } Question to the list:
} }
} } Our local safety inspector is concerned about the
} } appearance of the
} } fridge where we stock our solution of osmium. As
} you
} } can expect the
} } white interior of the fridge has turned grey with
} } vapors of osmium over
} } the years. Although we are taking every
} precautions
} } to avoid leakage of
} } osmium vapor from its container, this is a problem
} } that I have observed
} } in every EM lab that I have worked in. Does
} someone
} } on the list have a
} } method to store osmium in such a way as to avoid
} any
} } escape of vapor?
} } Also, is there something we could put into fridge
} } that would trap osmium
} } vapors as they leak out? Thanks for your advise.
} }
} } Marc
} }
} } --
} } Marc Pypaert
} } Department of Cell Biology
} } Center for Cell and Molecular Imaging
} } Ludwig Institute for Cancer Research
} } Yale University School of Medicine
} } 333 Cedar Street, PO Box 208002
} } New Haven, CT 06520-8002
} } TEL 203-785 3681
} } FAX 203-785 7446
} }
} }
} } ==============================Original
} } Headers==============================
} } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } 11:09:11 2005 5, 18 --
} } Received: from BIOMED.MED.YALE.EDU
} } (biomed.med.yale.edu
} } [130.132.232.48])
} } 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id
} } j72G9BnV011512
} } 5, 18 -- for {microscopy-at-microscopy.com} ; Tue, 2
} } Aug 2005
} } 11:09:11 -0500
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} } (net234-111.med.yale.edu
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} } 12:09:29 -0400 (EDT)
} } 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5,
} 18
} } -- From: Marc
} } Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} } Osmium vapors 5, 18 --
} } In-reply-to:
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} }
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 16, 29 -- From dmclea-at-sandia.gov Tue Aug 2
} 11:23:07
} } 2005
} } 16, 29 -- Received: from MM01SNLNTO.sandia.gov
} } (mm01snlnto.sandia.gov [132.175.109.20])
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} } with ESMTP id j72GN6sj016374
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} } 2005 10:22:57 -0600 (MDT)
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} } 16, 29 -- MIME-Version: 1.0
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} } 16, 29 -- Date: Tue, 2 Aug 2005 10:22:57 -0600
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5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:49:26 2005
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Working with undergraduates in a small (yet multiuser) lab, I have had
concerns about handling and storing osmium tetroxide for years. My
solution (which may not be practical for everyone) is that only sealed
ampoules are stored in the fridge (in their original shipping can and
packing materials). Vials are opened one at a time, the solutions are
mixed and used, tissues are exposed, left-over supplies are stored, and
waste is kept ONLY in the fume hood. In other words, opened supplies of
osmium never leave the fume hood (until disposal as toxic waste). If a
cold solution is needed, and ice bath can be used. We mix just about
enough for each procedure, and the unused left-over Os04 working
solution is kept in a small glass jar in the hood for the next
procedure. So we have no blackened refrigerator surfaces and no Os04
fumes accumulating in the fridge (or escaping into the room). It seems
to me that this approach could be expanded for a larger lab.

--Jan

---------------------------------------8/2/05
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College
State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------

marc.pypaert-at-yale.edu wrote:8/2/05
} ----------------------------------------------------------------------------
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}
} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
} 5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48])
} 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j72G9BnV011512
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
} 5, 18 -- In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
} 5, 18 -- To: microscopy-at-microscopy.com
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==============================Original Headers==============================
5, 22 -- From jfactor-at-ns.purchase.edu Tue Aug 2 11:51:39 2005
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5, 22 -- From: Jan Factor {jfactor-at-ns.purchase.edu}
5, 22 -- Subject: Re: [Microscopy] Osmium vapors
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Still alive and kikking in Houston.

hoffpajo-at-yahoo.com

08/02/2005 11:52 AM
Please respond to microscopy

To:
msteglic-at-mdanderson.org
cc:

is mannie Mannie Steglich still around? haven't heard
from him in a while.
john

--- hoffpajo-at-yahoo.com wrote:

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}
} in the alternative and it may be more expensive and
} you may not want to go there. and if someone with a
} better memory than i do anymore, can correct me. i
} do
} remember seeing premade OsO4 in small amounts. use
} what you need, perhaps even batch the tissue
} samples.
} then throw out the unused in the waste.
} just a thought.
}
} --- dmclea-at-sandia.gov wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
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} }
} } Marc
} }
} } We store our osmium in a secondary container...a
} } large poly pro bottle
} } (capped) and then we bag that container in a
} plastic
} } Ziploc. It helps a
} } bit.
} }
} } Dorrance
} }
} } -----Original Message-----
} } X-from: marc.pypaert-at-yale.edu
} } [mailto:marc.pypaert-at-yale.edu]
} } Sent: Tuesday, August 02, 2005 9:10 AM
} } To: McLean, Dorrance
} } Subject: [Microscopy] Osmium vapors
} }
} }
} }
} }
} }
}
------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
------------------------------------------------------------------------
} } ----
} }
} } Question to the list:
} }
} } Our local safety inspector is concerned about the
} } appearance of the
} } fridge where we stock our solution of osmium. As
} you
} } can expect the
} } white interior of the fridge has turned grey with
} } vapors of osmium over
} } the years. Although we are taking every
} precautions
} } to avoid leakage of
} } osmium vapor from its container, this is a problem
} } that I have observed
} } in every EM lab that I have worked in. Does
} someone
} } on the list have a
} } method to store osmium in such a way as to avoid
} any
} } escape of vapor?
} } Also, is there something we could put into fridge
} } that would trap osmium
} } vapors as they leak out? Thanks for your advise.
} }
} } Marc
} }
} } --
} } Marc Pypaert
} } Department of Cell Biology
} } Center for Cell and Molecular Imaging
} } Ludwig Institute for Cancer Research
} } Yale University School of Medicine
} } 333 Cedar Street, PO Box 208002
} } New Haven, CT 06520-8002
} } TEL 203-785 3681
} } FAX 203-785 7446
} }
} }
} } ==============================Original
} } Headers==============================
} } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } 11:09:11 2005 5, 18 --
} } Received: from BIOMED.MED.YALE.EDU
} } (biomed.med.yale.edu
} } [130.132.232.48])
} } 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id
} } j72G9BnV011512
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} } Aug 2005
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} } (net234-111.med.yale.edu
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} } 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005
} } 12:09:29 -0400 (EDT)
} } 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5,
} 18
} } -- From: Marc
} } Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} } Osmium vapors 5, 18 --
} } In-reply-to:
} } {200508021530.j72FU83w031285-at-ns.microscopy.com}
} } 5, 18 -- To: microscopy-at-microscopy.com
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} }
} }
} } ==============================Original
} } Headers==============================
} } 16, 29 -- From dmclea-at-sandia.gov Tue Aug 2
} 11:23:07
} } 2005
} } 16, 29 -- Received: from MM01SNLNTO.sandia.gov
} } (mm01snlnto.sandia.gov [132.175.109.20])
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29, 15 -- From msteglic-at-mdanderson.org Tue Aug 2 12:00:04 2005
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The simplest solution to this problem is not to keep your osmium in the
refrigerator. I keep mine in a glass Schott bottle with plastic cap which
is then stored within a plastic container in the fume hood. I am not sure
if light effects the osmium but to be sure, I wrap the plastic container in
aluminum foil. There is leakage from the glass bottle because over time,
the inside of the plastic container goes black. Anything that leaks past
the secondary container goes up the fume hood. Once every year or two, I
splurge and replace the bottle and container. I don't experience
"pre-mature" darkening of my 2% osmium stock which typically lasts about
2-3 months before I use it all up. Tom Phillips

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

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well thats good to know. is it cooler there than here?

--- msteglic-at-mdanderson.org wrote:

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} Still alive and kikking in Houston.
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}
}
}
} hoffpajo-at-yahoo.com
}
} 08/02/2005 11:52 AM
} Please respond to microscopy
}
}
}
}
} To:
} msteglic-at-mdanderson.org
} cc:
}
}
}
}
}
} Subject:
} [Microscopy] Re: Mannie Steglich
}
}
}
}
}
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}
} is mannie Mannie Steglich still around? haven't
} heard
} from him in a while.
} john
}
} --- hoffpajo-at-yahoo.com wrote:
}
} }
} }
} }
} }
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} }
} } in the alternative and it may be more expensive
} and
} } you may not want to go there. and if someone with
} a
} } better memory than i do anymore, can correct me. i
} } do
} } remember seeing premade OsO4 in small amounts. use
} } what you need, perhaps even batch the tissue
} } samples.
} } then throw out the unused in the waste.
} } just a thought.
} }
} } --- dmclea-at-sandia.gov wrote:
} }
} } }
} } }
} } }
} } }
} }
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} } }
} } } Marc
} } }
} } } We store our osmium in a secondary container...a
} } } large poly pro bottle
} } } (capped) and then we bag that container in a
} } plastic
} } } Ziploc. It helps a
} } } bit.
} } }
} } } Dorrance
} } }
} } } -----Original Message-----
} } } X-from: marc.pypaert-at-yale.edu
} } } [mailto:marc.pypaert-at-yale.edu]
} } } Sent: Tuesday, August 02, 2005 9:10 AM
} } } To: McLean, Dorrance
} } } Subject: [Microscopy] Osmium vapors
} } }
} } }
} } }
} } }
} } }
} }
}
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} } } ----
} } }
} } } Question to the list:
} } }
} } } Our local safety inspector is concerned about
} the
} } } appearance of the
} } } fridge where we stock our solution of osmium. As
} } you
} } } can expect the
} } } white interior of the fridge has turned grey
} with
} } } vapors of osmium over
} } } the years. Although we are taking every
} } precautions
} } } to avoid leakage of
} } } osmium vapor from its container, this is a
} problem
} } } that I have observed
} } } in every EM lab that I have worked in. Does
} } someone
} } } on the list have a
} } } method to store osmium in such a way as to avoid
} } any
} } } escape of vapor?
} } } Also, is there something we could put into
} fridge
} } } that would trap osmium
} } } vapors as they leak out? Thanks for your advise.
} } }
} } } Marc
} } }
} } } --
} } } Marc Pypaert
} } } Department of Cell Biology
} } } Center for Cell and Molecular Imaging
} } } Ludwig Institute for Cancer Research
} } } Yale University School of Medicine
} } } 333 Cedar Street, PO Box 208002
} } } New Haven, CT 06520-8002
} } } TEL 203-785 3681
} } } FAX 203-785 7446
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } } 11:09:11 2005 5, 18 --
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} } } (biomed.med.yale.edu
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} } } Aug 2005
}
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The last I knew he was at MD Anderson in Texas - but he may well have
retired by now - see http://woodenwonderstx.com/About.html

At 12:49 PM 8/2/2005, you wrote:

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tttan-at-simtech.a-star.edu.sg) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
August 1, 2005 at 20:24:16
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Email: tttan-at-simtech.a-star.edu.sg
Name: TT Tan

Organization: Singapore Inst. of Manuf. Tech.

Title-Subject: [Filtered] Field Ion Microscope

Question: Hi all,

I would like to know from who can I buy the glassware to do field ion
microscopy.

I am looking for a quartz ware that can do the job. Preferably one
that allows me to fit onto a NW40 joint.

Thanks in advance.

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (winston.wiggins-at-cshs.org) from
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Email: winston.wiggins-at-cshs.org
Name: Winston Wiggins

Organization: Cedars-Sinai HealthCare System

Title-Subject: [Filtered] MListserver: Osmium Vapors

Question: I keep my working solution of Osmium in a glass Wheaton or
Gibco bottle with a Teflon-lined cap and wrap the cap with Parafilm
and put that into the metal can that's shipped with the ampoules of
Osmium or a larger glass jar. I layer molecular sieves in the
can/jar. Any escaping Osmium vapors are indicated by blackening
Parafilm... or blackening refrigerator!
Our frig is clean.

Plastic is permeable to Osmium - use glass!

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Below is the result of your feedback form (NJZFM-ultra-55). It was
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Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

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Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

330-668-2235 Ext. 238

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NA is 0.5 of AA. Or more correctly NA = n(sine of (AA/2)). The notation n
is the refractive index between your lens and the glass slide or cover
slip, usually air n=1.0000. This isn't very helpful is it? AA or angular
aperture is a measure of the angle which describes the largest come of
light your objective will accept or your condenser will produce.
Resolution depends (simple theory) capturing as many of the defracted rays
from a sample as possible. The bigger the cone the more rays you capture
and the more resolution you have and the more you can magnify the sample.

Optics meant to be used in air always have an NA less than 1. Those using
oil, glycine or water have a NA greater than one. Condensers typically
have a higher NA than your assortment of objectives because they have to
accommodate many objectives. You reduce the NA of a condenser by closing
the condenser iris down. If you have the correct illumination set up you
can remove an eyepiece and watch the condenser iris close in the back focal
plane of the objective. Most of us close it a little past the edge of the
objective back focal plane 'cause we like it contrasty.

So your condenser is an meant to use in air while your objective is an oil
emersion. Never, never, say it with me, never put beans in your nose or
oil on a condenser with an NA of 0.9.

The best resolution you can get with your system is to oil the objective to
the slide and use the condenser in air. Frankly, it's just OK that way and
in my opinion it's oil immersion is never worth the work (OK you can flame
me now...) You need to oil both the condenser and objective to the slide
(assuming you have the right thickness slide, cover slip and your sample
has sufficient contrast by staining or difference in refractive index) to
get the most out of your optical system. I'm a microscopist cause I'm a
big sloppy guy and this is all too much trouble for me.

Sound like a homework question, so good luck!!!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

330-668-2235 Ext. 238

This e-mail transmission, and any documents, files or previous e-mail
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attachments without reading or saving in any manner. Thank you.

sswaffe-at-abv.bg
To: frank.karl-at-degussa.com
08/02/2005 02:28 cc:
PM Subject: [Microscopy] AskAMicroscopist: numerical aperture
Please respond to
microscopy

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on Tuesday, August 2, 2005 at 12:24:49
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

---------------------------------------------------------------------------

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Dear Sophia,

In air a na of .9 is all that is possible with out oiling the
condenser to the slide. Condensers made with .9 na make better
images with out oil than 1.2 na to 1.4 na oil immersion
condensers with out oil. Also simple Abby condensers have a good
deal of chromic aberration at wide apertures.

The combination of those and the fact that most users don't take
the time to oil the condenser to the bottom of the slide
resulted in many microscope makers using .9 na condensers. For a
in depth discussion of the problems of large na condenser and
other things see Ted Clark's piece
http://www.modernmicroscopy.com/main.asp?article=53 in Modern
Microscopy. Other work on lighting by Ted Clarke is referenced
at http://www.couger.com/microscope/Ted-Clarke/

You asked a good questioning about the aperture of the
condenser. To get the full performance form an objective the
condenser and light train need to be the same quality and na as
the objective. That is usually only a real consideration for
study at high resolution and high resolution microphotography.
The limiting factor in any optic system is its weakest link. In
micosopes this often the lighting and/or condeser.

Best Regards
Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org
sswaffe-at-abv.bg wrote:

} Email: sswaffe-at-abv.bg
} Name: Veselin Andreev
}
} Organization: National High School of Mathematics and Science
}
} Education: 9-12th Grade High School
}
} Location: Sofia, Bulgaria
}
} Question: What exactly is the N.A. or numerical aperture and
why on
} my condensor it's value is 0.9 and on my high power objetive
it is
} 1.25?

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The numerical aperture is a rating for how much light comes through. A
variety of design factors that I can't explain affect the value. An
excellent source of information for your class is available on the Olympus
site. I've pasted the address of the page discussing objectives and N.A.
below. From there you can navigate throughout their entire primer.

http://olympusmicro.com/primer/anatomy/objectives.html

I don't have any connection to Olympus and other manufacturers may also have
excellent technical information up on the web. I suggested this site
because I found it to be very well organized, illustrated, and easy to
understand. Aside from the liberal use of the "Olympus" label, the
discussions are based on science, not a sales pitch. I hope this helps.

Walt Klonowski
Pikes Peak Test Labs

-----Original Message-----
X-from: sswaffe-at-abv.bg [mailto:sswaffe-at-abv.bg]
Sent: Tuesday, August 02, 2005 12:31 PM
To: WaltK-at-pptli.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sswaffe-at-abv.bg) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 2, 2005 at 12:24:49
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

---------------------------------------------------------------------------

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Dear Veselin,

The numerical aperture is the light collecting ability of a specific piece of optics. It depends on two factors: half the actual collecting angle and the refractive index of the immersion fluid. The equation is:
NA=n x sin a where n = ri of the immersion fluid (air is 1.00, oil is 1.5212, water is 1.33, for example) and " a" is the half angle.

Your question is an interesting one for several reasons.

First, both the NA of the condenser and the NA of the objective contribute to resolution: (Equations are difficult to send by email, but here is the basic equation:)
R = [1.2 L]/[NAo + NAc] where 1.2 is a shape factor (Bessel function) reflecting the round apertures we use in the microscope, "L" is the wavelength of light (you can use 500nm or 0.500microns as an average), and NAo = NA objective; NAc = NA condenser.

Interestingly, this equation is related to not only the ability to resolve spacing (R is actually the smallest distance between two objects which still permits them to be imaged as 2 separate entities), it also has impact on edge fidelity. As a result, higher NA optics (both objective and condenser) not only improve your ability to resolve fine detail, they also produce crisper, sharper edges.

Secondly, the NA's written on your glassware are only the general operating specs. If you close down the iris in the condenser, you limit the NA of the condenser. Since your condenser has a maximum NA of 0.9,it is not meant to be used with oil. On the other hand, your objective NA indicates that, for best imaging, a drop of the appropriate immersion oil should be placed between it and the top of your sample. If you don't follow this procedure, your images will not only lack clarity (immersion oil is considered to be an important optical component of the system), your objective will only operate with a "working NA" of approximately 0.9.

Finally, your question is very appropriate because, for optimum resolution, the NA of the condenser should match or exceed the NA of your objective. Your condenser does not meet this requirement. If you do the calculations with the Resolution equation above, you will see that you will limit the resolution available from your system. Since you are working at the High School level, this limitation will probably not have a serious impact on your work. If you eventually get involved with higher level research, I would recommend that you purchase a condenser which better fits your needs.

All of this is explained in "Optimizing Light Microscopy," a book still available through MME. For further information, please contact Ken Piel at kenpiel-at-mme1.com. (Tell him I sent you).

Hope this is helpful and good luck in your studies!

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.

At 03:30 PM 8/2/2005, waltk-at-pptli.com wrote:

} ----------------------------------------------------------------------------
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Hello to you all,

We keep our osmium solutions in Schott Duran laboratory bottles
(http://www.schott-duran.com/english/products/duran/detail/laborflaschen
.html), and keep the bottles in a paint can (a new one that never had
paint in it). This is then kept in our fridge. This arrangement seems to
keep the vapour contained (no marks in our fridge) and isn't a pain to
open up. I also keep a pottle of whole milk powder in there for spills.

Aaron Hicks
Electron Microscopy Preparation Technician

Comparative Physiology and Anatomy
Institute of Veterinary, Animal, and Biomedical Sciences
Massey University

PN-412
Private Bag 11 222
Palmerston North
New Zealand

Phone +64 06 350 4874

-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Wednesday, 3 August 2005 4:12 a.m.
To: Hicks, Aaron

Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey with vapors
of osmium over the years. Although we are taking every precautions to
avoid leakage of osmium vapor from its container, this is a problem that
I have observed in every EM lab that I have worked in. Does someone on
the list have a method to store osmium in such a way as to avoid any
escape of vapor? Also, is there something we could put into fridge that
would trap osmium vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

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Osmium vapours are not stopped by simple bottle tops. We buy ours
already made up. The bottle lids have a plastic (?) insert which is
effective.

Waste osmium is stored in any bottle which is then kept in what we can
in the UK a Kilner jar. It is made of glass, has a rubber seal and uses
wire to clamp the lid very tightly. Osmium vapours do not seem to pass
through rubber seals. Unfortunately new Kilner jars in my supermarket
have plastic seals - so an experiment will be required.

Dave

-----Original Message-----
X-from: gunkelrl-at-slu.edu [mailto:gunkelrl-at-slu.edu]
Sent: 02 August 2005 17:49
To: David Patton

We use Osmium in our lab and keep it in the refrigerator.
We keep it in a glass bottle and cover the lid with
parafilm. Then we put that bottle in another bottle with a
screw top and wrap the bottle in foil. It works great, our
refrigerator doesn't have and black residue, just the two
bottles.

rebecca

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Dear Veselin, dear list members,

appart from the good explanations you already received from other list
members, I recommend you another link where you can also find explanations
on how the NA determines the microscope resolution:

http://support.svi.nl/wiki/NumericalAperture

As this is a wiki site, you can also share your knowledge by editing it!

Regards,

jose.

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Hola

Homeowner

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Good Day,

Rachelle Mayo

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Dear Nestor,

will replies like these reach the sender? I mean, this question was posted
from AskAMicroscopist, not emailed by a list member, so I am afraid our
replies will not reach people like Veselin unless we explicity include
them in the address of our emails...

jose.

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Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html

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Hi, Skage

We have a consultant who has a lot of experience in this area and also provides training. I"ll forward your email to him

Best regards
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.

At 10:08 AM 8/3/2005, SHem-at-laurentian.ca wrote:

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Skage,

We have been running an older version, S200 for some 15 years. Overall, it
has had an excellent service history (mostly replacing vacuum pumps and
occasional chips, resistors, capacitors, etc).

We are a metallurgy lab, so our samples tend to be conductive and dry. The
large sample chamber is a real plus as is the nice stage. Also, we are not
heavy users and kept limited access with regard to number of users. We do
miss having direct digital imaging, which I presume the S250 has. Your
situation may be very different than ours. Newer scopes offer partial
vacuum capabilities and probably better ultimate resolution.

Other than being an older instrument (not always a bad thing) without
some of the newer bells and whistles, I have no regrets and we look forward
to many more years of service from it.

Alan Stone
ASTON

At 10:07 AM 8/3/2005, you wrote:

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Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com

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I had known at one time, but I suppose due to age, I can't remember, but I'm
hoping that people here could advise me as to the best knife angle to buy on
a Diatome knife. All our specimens are human biopsy tissue embedded in
epon. I'd like to buy a histo-knife, and I was under the impression that 45
degrees would be the best sort of knife angle for routine use like this. Is
this correct?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

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In North America, Kilner jars are called Mason jars.

--
Lesley Weston

} From: David.Patton-at-uwe.ac.uk
} Reply-To: microscopy-at-microscopy.com
} Date: Wed, 03 Aug 2005 06:26:13 -0500
} To: leswes-at-shaw.ca
} Subject: [Microscopy] Osmium vapors
}
}
}
}
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} Osmium vapours are not stopped by simple bottle tops. We buy ours
} already made up. The bottle lids have a plastic (?) insert which is
} effective.
}
} Waste osmium is stored in any bottle which is then kept in what we can
} in the UK a Kilner jar. It is made of glass, has a rubber seal and uses
} wire to clamp the lid very tightly. Osmium vapours do not seem to pass
} through rubber seals. Unfortunately new Kilner jars in my supermarket
} have plastic seals - so an experiment will be required.
}
} Dave
}
} -----Original Message-----
} X-from: gunkelrl-at-slu.edu [mailto:gunkelrl-at-slu.edu]
} Sent: 02 August 2005 17:49
} To: David Patton
} Subject: [Microscopy] Re: Osmium vapors
}
}
}
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} We use Osmium in our lab and keep it in the refrigerator.
} We keep it in a glass bottle and cover the lid with
} parafilm. Then we put that bottle in another bottle with a
} screw top and wrap the bottle in foil. It works great, our
} refrigerator doesn't have and black residue, just the two
} bottles.
}
} rebecca
}
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I use a 45 for normal work. I find it works very well. I have a 35 I
use for special work. 35 has some advantages, but is not as robust.
David

On Aug 3, 2005, at 11:41 AM, GBurgess-at-exchange.hsc.mb.ca wrote:

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} I had known at one time, but I suppose due to age, I can't remember,
} but I'm
} hoping that people here could advise me as to the best knife angle to
} buy on
} a Diatome knife. All our specimens are human biopsy tissue embedded in
} epon. I'd like to buy a histo-knife, and I was under the impression
} that 45
} degrees would be the best sort of knife angle for routine use like
} this. Is
} this correct?
}
} This e-mail and/or any documents in this transmission is intended for
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5, 22 -- From Elliott-at-arizona.edu Wed Aug 3 13:58:43 2005
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it has been a while since i have purchased a knife. 48
degrees should work well for biological tissue.
if you have doubts, questions or concerns i recommend
contacting staci kirsh at electron microscopy
sciences, she is an invaluble resource.
john

--- GBurgess-at-exchange.hsc.mb.ca wrote:

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} I had known at one time, but I suppose due to age, I
} can't remember, but I'm
} hoping that people here could advise me as to the
} best knife angle to buy on
} a Diatome knife. All our specimens are human biopsy
} tissue embedded in
} epon. I'd like to buy a histo-knife, and I was
} under the impression that 45
} degrees would be the best sort of knife angle for
} routine use like this. Is
} this correct?
}
} This e-mail and/or any documents in this
} transmission is intended for the address(s) only and
} may contain legally privileged or confidential
} information. Any unauthorized use, disclosure,
} distribution, copying or dissemination is strictly
} prohibited. If you receive this transmission in
} error, please notify the sender immediately and
} return the original.
}
} ==============================Original
} Headers==============================
} 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Wed Aug 3
} 13:35:20 2005
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} 3, 20 -- From: Garry Burgess
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__________________________________________________
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I have had 45 degree diamonds for most of my career but last year bought a
35 degree one. I think there is a significant improvement for the lymphoid
tissue we are cutting these days. I hear they wear out faster but I don't
do a ton of sectioning so the tradeoff seems worth it. Tom

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

==============================Original Headers==============================
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I have received your message, but will be out of the office until August 4th and will not be able to reply until then.

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lambert-at-jeol.com wrote:

} I have received your message, but will be out of the office

I apologize to the list for the unnecessary messages. We have recently started using the automated reply feature of our email server with some unexpected results. Attempts to repair the auto-reply logic have (so far) failed. Hopefully, my unsubscribe request will be processed soon. Again, I apologize for wasted bandwidth.

Best regards,
Mike Lambert

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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html

==============================Original Headers==============================
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31, 23 -- From kenconverse-at-qualityimages.biz Wed Aug 3 17:04:21 2005
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31, 23 -- To: {microscopy-at-microscopy.com}
31, 23 -- Subject: RE: [Microscopy] Cambridge S-250
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Hi Ken, Its replacing a nanolab-7 (old Zeiss)
Skage

} } } kenconverse-at-qualityimages.biz 08/03/05 6:08 PM } } }

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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html

==============================Original Headers==============================
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16, 17 -- From: "Skage Hem" {SHem-at-laurentian.ca}
16, 17 -- To: {microscopy-at-microscopy.com}
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31, 23 -- From kenconverse-at-qualityimages.biz Wed Aug 3 17:04:21 2005
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Contents Retrieved from Microscopy Listserver Archives
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Diatome suggest for their 'ultra' diamond knives, that 45 degree is
good for routine sections. 35 degree apparently offers less section
distortions in biology and materials science. 55 degree is best for
really hard ceramics and similar materials

The Diatome article offers two references:
1. J.C. Jesior; How to avoid compression; Jnl. of Ultrastructure &
Molecular Structure Res. 95, 210-217 (1986)
2. J.C. Jesior; Use of low-angle diamond knives leads to improved
ultrastructural preservation of ultrathin sections; Scanning Microscopy
Supplement 3, 147-153 (1989); Scanning Microscopy International,
Chicago (AMF O'Hare) IL 6066 USA.

This information came out of a Diatome colour brochure which I must
have received some time in the last 10 years or so. I do not have the
actual references.

NB Incidentally Diatome do market a 'Histo' knife but it is really
intended for light microscopy sections rather than ultrathin ones
('Ultra' knives).

Good luck

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
X-from: GBurgess-at-exchange.hsc.mb.ca

unless i have forgotten, diatome also/used to market a
semi diamone kife that would cut semi thin sections as
well as thin sections. of course the memory is the
first thing to go.

--- malcolm.haswell-at-sunderland.ac.uk wrote:

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} Diatome suggest for their 'ultra' diamond knives,
} that 45 degree is
} good for routine sections. 35 degree apparently
} offers less section
} distortions in biology and materials science. 55
} degree is best for
} really hard ceramics and similar materials
}
} The Diatome article offers two references:
} 1. J.C. Jesior; How to avoid compression; Jnl. of
} Ultrastructure &
} Molecular Structure Res. 95, 210-217 (1986)
} 2. J.C. Jesior; Use of low-angle diamond knives
} leads to improved
} ultrastructural preservation of ultrathin sections;
} Scanning Microscopy
} Supplement 3, 147-153 (1989); Scanning Microscopy
} International,
} Chicago (AMF O'Hare) IL 6066 USA.
}
} This information came out of a Diatome colour
} brochure which I must
} have received some time in the last 10 years or so.
} I do not have the
} actual references.
}
} NB Incidentally Diatome do market a 'Histo' knife
} but it is really
} intended for light microscopy sections rather than
} ultrathin ones
} ('Ultra' knives).
}
} Good luck
}
} Malcolm
}
} Malcolm Haswell
} e.m. unit
} School of Health, Natural and Social Sciences
} Fleming Building
} University of Sunderland
} Tyne & Wear
} SR1 3SD
} UK
} e-mail: malcolm.haswell-at-sunderland.ac.uk
}
}
} ----- Original Message -----
} X-from: GBurgess-at-exchange.hsc.mb.ca
} Date: Wednesday, August 3, 2005 7:41 pm
} Subject: [Microscopy] best knife angle
}
} }
} }
} }
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} }
} } I had known at one time, but I suppose due to age,
} I can't
} } remember, but I'm
} } hoping that people here could advise me as to the
} best knife angle
} } to buy on
} } a Diatome knife. All our specimens are human
} biopsy tissue
} } embedded in
} } epon. I'd like to buy a histo-knife, and I was
} under the
} } impression that 45
} } degrees would be the best sort of knife angle for
} routine use like
} } this. Is
} } this correct?
} }
} } This e-mail and/or any documents in this
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} 2005
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} } 3, 20 -- From: Garry Burgess
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} Aug 4 03:39:20 2005
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} (qpsmtpd/0.28) with ESMTP; Thu, 04 Aug 2005 09:39:09
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Contents Retrieved from Microscopy Listserver Archives
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Skage,
I'm not really familiar with either model, but maybe someone on the list is
and can give you some recommendations. You might also try contacting Earl
Weltmer of Scanservice Corp.
earlw-at-sbcglobal.net
as he is familiar with more makes and models than I am.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: shem-at-laurentian.ca [mailto:shem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 8:44 PM
To: kenconverse-at-qualityimages.biz

Hi Ken, Its replacing a nanolab-7 (old Zeiss)
Skage

} } } kenconverse-at-qualityimages.biz 08/03/05 6:08 PM } } }

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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html

==============================Original Headers==============================
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16, 17 -- From: "Skage Hem" {SHem-at-laurentian.ca}
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31, 23 -- From kenconverse-at-qualityimages.biz Wed Aug 3 17:04:21 2005
31, 23 -- Received: from qualityimages.biz (dpmail16.doteasy.com
[65.61.209.16])
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31, 23 -- To: {microscopy-at-microscopy.com}
31, 23 -- Subject: RE: [Microscopy] Cambridge S-250
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53, 23 -- From kenconverse-at-qualityimages.biz Thu Aug 4 09:29:41 2005
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thanks for the info, mannie is still around and hasn't
retired yet. i just got an email from him. we went to
college together. i can't tell you the number of
friends and girl friends i lost touch with from
college. some i regret others i do not.
so are you heading to st martins?

--- microbill-at-mohawk.net wrote:

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} The last I knew he was at MD Anderson in Texas - but
} he may well have
} retired by now - see
} http://woodenwonderstx.com/About.html
}
}
}
}
} At 12:49 PM 8/2/2005, you wrote:
}
}
}
}
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} }
} } is mannie Mannie Steglich still around? haven't
} heard
} } from him in a while.
} } john
} }
} } --- hoffpajo-at-yahoo.com wrote:
} }
} } }
} } }
} } }
} } }
}
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} } }
} } } in the alternative and it may be more expensive
} and
} } } you may not want to go there. and if someone
} with a
} } } better memory than i do anymore, can correct me.
} i
} } } do
} } } remember seeing premade OsO4 in small amounts.
} use
} } } what you need, perhaps even batch the tissue
} } } samples.
} } } then throw out the unused in the waste.
} } } just a thought.
} } }
} } } --- dmclea-at-sandia.gov wrote:
} } }
} } } }
} } } }
} } } }
} } } }
} } }
}
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} } } }
} } } } Marc
} } } }
} } } } We store our osmium in a secondary
} container...a
} } } } large poly pro bottle
} } } } (capped) and then we bag that container in a
} } } plastic
} } } } Ziploc. It helps a
} } } } bit.
} } } }
} } } } Dorrance
} } } }
} } } } -----Original Message-----
} } } } X-from: marc.pypaert-at-yale.edu
} } } } [mailto:marc.pypaert-at-yale.edu]
} } } } Sent: Tuesday, August 02, 2005 9:10 AM
} } } } To: McLean, Dorrance
} } } } Subject: [Microscopy] Osmium vapors
} } } }
} } } }
} } } }
} } } }
} } } }
} } }
}
} ------------------------------------------------------------------------
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} } } } The Microscopy ListServer -- CoSponsor: The
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}
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} } } } ----
} } } }
} } } } Question to the list:
} } } }
} } } } Our local safety inspector is concerned about
} the
} } } } appearance of the
} } } } fridge where we stock our solution of osmium.
} As
} } } you
} } } } can expect the
} } } } white interior of the fridge has turned grey
} with
} } } } vapors of osmium over
} } } } the years. Although we are taking every
} } } precautions
} } } } to avoid leakage of
} } } } osmium vapor from its container, this is a
} problem
} } } } that I have observed
} } } } in every EM lab that I have worked in. Does
} } } someone
} } } } on the list have a
} } } } method to store osmium in such a way as to
} avoid
} } } any
} } } } escape of vapor?
} } } } Also, is there something we could put into
} fridge
} } } } that would trap osmium
} } } } vapors as they leak out? Thanks for your
} advise.
} } } }
} } } } Marc
} } } }
} } } } --
} } } } Marc Pypaert
} } } } Department of Cell Biology
} } } } Center for Cell and Molecular Imaging
} } } } Ludwig Institute for Cancer Research
} } } } Yale University School of Medicine
} } } } 333 Cedar Street, PO Box 208002
} } } } New Haven, CT 06520-8002
} } } } TEL 203-785 3681
} } } } FAX 203-785 7446
} } } }
} } } }
} } } } ==============================Original
} } } } Headers==============================
} } } } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } } } 11:09:11 2005 5, 18 --
} } } } Received: from BIOMED.MED.YALE.EDU
} } } } (biomed.med.yale.edu
} } } } [130.132.232.48])
} } } } 5, 18 -- by ns.microscopy.com
} (8.12.11/8.12.8)
} } } with
} } } } ESMTP id
} } } } j72G9BnV011512
} } } } 5, 18 -- for {microscopy-at-microscopy.com} ;
} Tue, 2
} } } } Aug 2005
} } } } 11:09:11 -0500
} } } } 5, 18 -- Received: from yale.edu
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} } } } [130.132.234.111]) 5, 18 -- by
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} } } } 12:09:29 -0400 (EDT)
}
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5, 19 -- From hoffpajo-at-yahoo.com Thu Aug 4 10:52:35 2005
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Fellow listers:

Just a reminder that the "Reply" functionality of the
list has recently changed.

Currently, when you "Reply" to a message, the e-mail
is sent to the entire list, not just the original
sender. I believe that this is/was a biproduct of
Nestor's efforts to minimize SPAM (great job Nestor,
keep up the good work).

This is different than in the past, when a "Reply"
only went to the author of the post you were replying
to.

While this makes it easier to reply and have a post go
to the group, it also seems to have spawned a number
of messages there were meant for specific individuals,
but made their way to the entire group.

Not necessarily a problem, but in some cases it could
be...

John W. Raffensperger, Jr.
Beaver Dam, Wisconsin, USofA

==============================Original Headers==============================
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I noticed the Tungsten Carbide knives in a catalog, and wondered if this
sort of knife would be at all suitable to cut 0.7 micron sections of Epon
embedding biological material. Currently we are using histo-diamond knives,
but it is my dream to be able to use a more durable, cheaper knife that
gives the same result. I was just wondering if anyone has tried this, and
what sort of result they might get with epon. Or is it a stupid idea?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

==============================Original Headers==============================
3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Thu Aug 4 12:07:31 2005
3, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122])
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3, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
3, 20 -- Subject: Tungsten Carbide Knives and Semi-thin Epon Sections
3, 20 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
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Although I am only familiar with so-called 'hard' materials microtomy
(metals, alloys, minerals, ceramic fibers and the like) the interesting
point about the knife angle in this area is that the 35 sections better for
all of the above materials in my lab, so the 45 knives gather dust. Since
wear is a function of the amount of sectioning as well as type of material,
I cannot comment on that aspect, but can note that we never had any damage
to the 35 except when we deliberately tried to do so on ~20 micron,
extremely hard amorphous alloys particles. (It was not pretty!).

The success of the lower angle is no fluke, as Helmut Gnaegi of Diatome has
gotten good sections of; 200 micron quartz particles, polysilicon/metal
layers on a single crystal silicon substrate, carbon fibers (but care had to
be used or nicks would occur), superconducting oxide and Ti implants in
bone. Phil Swab (with a coatings company in California) has sectioned many
optical glass coatings, and layers of boron nitride and artificial diamond
on single crystal silicon substrate. To the best of my knowledge, both of
these specialists had trouble with any other angles.

My theory is that, for most of the above examples, the knife edge only
initiates a crack in the fairly brittle material which is then 'wedged open'
across the block face by the angle of the knife, hence the reduced damage
with reduced angle (though section breakup does occur).

Finally, we did a bit of fooling around with histo knives a few years back.
Far from being only good for semithins, though 1 micron thick sections of
aluminum were produced, they produced some of the thinnest, flattest
sections we ever obtained (~10 nm for nanocrystalline metals) and was the
only knife that produced decent ~20 nm thick sections of the above amorphous
alloy particles. Go figure.

Tom

Dr. Tom Malis
Manager / Gestionnaire
Academic User Access Facility (AUAF) / La Facilit d’accs aux utilisateurs
universitaire (FAUU)
CANMET Materials Technology Laboratory / LTM-CANMET
Natural Resources Canada / Ressources naturelles Canada
568 Booth St. / 568 rue Booth, Ottawa, Ontario K1A 0G1
Tel.: (613) 995-1493 FAX: (613) 992-8735; cell: 613-371-4577
malis-at-nrcan.gc.ca

-----Original Message-----
X-from: phillipst-at-missouri.edu [mailto:phillipst-at-missouri.edu]
Sent: August 3, 2005 3:38 PM
To: malis-at-nrcan.gc.ca

I have had 45 degree diamonds for most of my career but last year bought a
35 degree one. I think there is a significant improvement for the lymphoid
tissue we are cutting these days. I hear they wear out faster but I don't
do a ton of sectioning so the tradeoff seems worth it. Tom

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

==============================Original Headers==============================
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the histo knife should last for years in the hands of
a careful tech. and if you are using the sections for
orientation a few knife scratches should matter. just
be sure to not let an inexperienced person use them.

--- GBurgess-at-exchange.hsc.mb.ca wrote:

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}
} I noticed the Tungsten Carbide knives in a catalog,
} and wondered if this
} sort of knife would be at all suitable to cut 0.7
} micron sections of Epon
} embedding biological material. Currently we are
} using histo-diamond knives,
} but it is my dream to be able to use a more durable,
} cheaper knife that
} gives the same result. I was just wondering if
} anyone has tried this, and
} what sort of result they might get with epon. Or is
} it a stupid idea?
}
} This e-mail and/or any documents in this
} transmission is intended for the address(s) only and
} may contain legally privileged or confidential
} information. Any unauthorized use, disclosure,
} distribution, copying or dissemination is strictly
} prohibited. If you receive this transmission in
} error, please notify the sender immediately and
} return the original.
}
} ==============================Original
} Headers==============================
} 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Thu Aug 4
} 12:07:31 2005
} 3, 20 -- Received: from hscxntmx0003.hsc.mb.ca
} (hscxntmx0003.hsc.mb.ca [142.233.100.122])
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{00A937989100304A83A058F6C45873FF32A253-at-hscxntmx0005.hsc.mb.ca}
} 3, 20 -- Date: Thu, 4 Aug 2005 12:06:02 -0500
} 3, 20 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
} 3, 20 -- Subject: Tungsten Carbide Knives and
} Semi-thin Epon Sections
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I have used the Tungsten Carbide knives some. They are not sharp enough to
cut sub-micron thick sections as they leave a slight snowy appearance to the
block face. I have been successful in cutting plastic embedded tissue
sections of approximately 3-5 microns with them. The edge stays good much
longer than glass does. They are great for working with harder materials.
After applying per-mount and coversliping the sections, the snowiness is no
longer evident.

===========================================
Michael D. Standing
Microscopy Technician
Brigham Young University Microscopy Lab
A-125A CLFB
Provo, UT 84602

Phone: (801) 422-4011
E-Mail: Michael_Standing-at-byu.edu
===========================================

-----Original Message-----
X-from: GBurgess-at-exchange.hsc.mb.ca [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Thursday, August 04, 2005 11:09 AM
To: Michael_Standing-at-byu.edu

I noticed the Tungsten Carbide knives in a catalog, and wondered if this
sort of knife would be at all suitable to cut 0.7 micron sections of Epon
embedding biological material. Currently we are using histo-diamond knives,
but it is my dream to be able to use a more durable, cheaper knife that
gives the same result. I was just wondering if anyone has tried this, and
what sort of result they might get with epon. Or is it a stupid idea?

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12, 21 -- From Michael_Standing-at-byu.edu Thu Aug 4 12:58:57 2005
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12, 21 -- From: "Michael Standing" {Michael_Standing-at-byu.edu}
12, 21 -- To: {microscopy-at-microscopy.com}
12, 21 -- Subject: RE: [Microscopy] Tungsten Carbide Knives and Semi-thin Epon Sections
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Another couple reminder about recent changes-

First, it used to be that a poster's personal name would appear in the
X-from: field and give a pretty good idea of their identity. (List rules
(http://www.msa.microscopy.org/MicroscopyListserver/Rules.html) ask that we
provide our real name as part of the post.) Recent changes have stripped
that field down to just the e-mail address. That makes messages look a lot
more anonymous than before. In fact, the original From: field is preserved
in the new stuff tacked on to the bottom of the post, but many of us
probably don't look those lines over. So, it is probably a good practice to
add a brief signature line so we clearly know who you are and can avoid
some of the recent confusion.

Second, there are those new lines of stuff tacked onto the end of the
posts. I encourage you all to trim those back as you reply to messages.
They add unnecessary bulk to the messages. A new set is always tacked on
anyway.

Cheers, even to those of you in Hawaii. I almost joined you there.

Warren Straszheim
Iowa State University

At 11:11 AM 08/04/05, you wrote:

} Fellow listers:
}
} Just a reminder that the "Reply" functionality of the
} list has recently changed.
}
} Currently, when you "Reply" to a message, the e-mail
} is sent to the entire list, not just the original
} sender. I believe that this is/was a biproduct of
} Nestor's efforts to minimize SPAM (great job Nestor,
} keep up the good work).
}
} This is different than in the past, when a "Reply"
} only went to the author of the post you were replying
} to.
}
} While this makes it easier to reply and have a post go
} to the group, it also seems to have spawned a number
} of messages there were meant for specific individuals,
} but made their way to the entire group.
}
} Not necessarily a problem, but in some cases it could
} be...
}
} John W. Raffensperger, Jr.
} Beaver Dam, Wisconsin, USofA

==============================Original Headers==============================
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On Aug 4, 2005, at 1:03 PM, Michael_Standing-at-byu.edu wrote:

}
}
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}
} I have used the Tungsten Carbide knives some. They are not sharp
} enough to
} cut sub-micron thick sections as they leave a slight snowy
} appearance to the
} block face. I have been successful in cutting plastic embedded tissue
} sections of approximately 3-5 microns with them. The edge stays
} good much
} longer than glass does. They are great for working with harder
} materials.
} After applying per-mount and coversliping the sections, the
} snowiness is no
} longer evident.
}
} ===========================================
} Michael D. Standing
} Microscopy Technician
} Brigham Young University Microscopy Lab
} A-125A CLFB
} Provo, UT 84602
}
} Phone: (801) 422-4011
} E-Mail: Michael_Standing-at-byu.edu

Exactly, Michael.

The problem with tungsten carbide is that it is actually a
microcrystalline alloy of tungsten carbide particles bonded with
cobalt, and this microstructure structure interferes with very thin
sections, much as with steel, but the carbide is harder and tougher,
which is why it is used to machine steel.

Knife facets may look quite shiny but they need to be polished much
flatter than a wavelength of visible light if they are to meet at a
smooth edge measured in the low nanometers, and thus be capable of
cutting the sub-100 nanometer sections appropriate for EM work.

The good thing about metal alloys is that the edge angle can be made
more acute while retaining the desired durability, but only at the
expense of minimum section thickness, so they are used for LM work.

Glass does well for EM knives because it is amorphous and thus has no
microstructure, while being hard enough to section. Of the hard
monocrystal alternatives to glass suitable for EM knives, only
sapphire, silicon carbide and diamond seem appropriate. All these are
good candidate materials for ultramicrotome knives, depending on cost
and other requirements.

Roger Baker
1303 Bentwood,
Austin, Tx,
78722

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12, 19 -- Subject: Re: [Microscopy] RE: Tungsten Carbide Knives and Semi-thin Epon Sections
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Greetings,
I am looking for a tech that used to work here at UMBC, his name is
Phil Rutledge. If anyone knows his whereabouts could you have him e-mail
me at cpetty1-at-umbc.edu. He is a whiz with both our zeiss and jeol and I
would love to ask him some questions about the scopes and stuff I have
found lying about the facility.
Thanks
--
Chere Petty, M.S.
Manager, Keith R. Porter Imaging Facility
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
1000 Hilltop Circle
Baltimore, MD 21250
Phone: 410-455-2296
Fax: 410-455-3875

==============================Original Headers==============================
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1, 21 -- From: Chere Petty {cpetty1-at-umbc.edu}
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Glass Knives, cheap fast, reliable, Histo Diamonds are excellent and durable
if used with care!
Markus F. Meyenhofer
Microscopy Labs.
----- Original Message -----
X-from: {GBurgess-at-exchange.hsc.mb.ca}
To: {micro-at-superlink.net}
Sent: Thursday, August 04, 2005 1:07 PM

yes i agree, glass knives are relativly cheap, fast?
depends on what you are using them for. histo knives
should last for years and can be used to face the
blocks as well. used one for years untill someone with
little experience got hold of it, i had never seen a
diamond with so many chips in it.
moral of the story, keep your knives close, but keep
your diamind knives to yourself.

--- micro-at-superlink.net wrote:

}
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}
} Glass Knives, cheap fast, reliable, Histo Diamonds
} are excellent and durable
} if used with care!
} Markus F. Meyenhofer
} Microscopy Labs.
} ----- Original Message -----
} X-from: {GBurgess-at-exchange.hsc.mb.ca}
} To: {micro-at-superlink.net}
} Sent: Thursday, August 04, 2005 1:07 PM
} Subject: [Microscopy] Tungsten Carbide Knives and
} Semi-thin Epon Sections
}
}
} }
} }
} }
} }
}
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} }
} }
} } I noticed the Tungsten Carbide knives in a
} catalog, and wondered if this
} } sort of knife would be at all suitable to cut 0.7
} micron sections of Epon
} } embedding biological material. Currently we are
} using histo-diamond
} } knives,
} } but it is my dream to be able to use a more
} durable, cheaper knife that
} } gives the same result. I was just wondering if
} anyone has tried this, and
} } what sort of result they might get with epon. Or
} is it a stupid idea?
} }
} } This e-mail and/or any documents in this
} transmission is intended for the
} } address(s) only and may contain legally privileged
} or confidential
} } information. Any unauthorized use, disclosure,
} distribution, copying or
} } dissemination is strictly prohibited. If you
} receive this transmission in
} } error, please notify the sender immediately and
} return the original.
} }
} } ==============================Original
} } Headers==============================
} } 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Thu Aug
} 4 12:07:31 2005
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{00A937989100304A83A058F6C45873FF32A253-at-hscxntmx0005.hsc.mb.ca}
} } 3, 20 -- Date: Thu, 4 Aug 2005 12:06:02 -0500
} } 3, 20 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
} } 3, 20 -- Subject: Tungsten Carbide Knives and
} Semi-thin Epon Sections
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} 20:14:33 2005
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We use bottles with rubber septums to store the liquid, and keep the
bottles within sealed falcon tubes, within a sealed bottle. This keeps
the vapours from leaking and discolouring the fridge.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Tue, 2 Aug 2005, marc.pypaert-at-yale.edu wrote:

}
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} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
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}

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4, 26 -- From gvrdolja-at-nature.berkeley.edu Fri Aug 5 12:35:47 2005
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4, 26 -- From: Gordon Vrololjak {gvrdolja-at-nature.berkeley.edu}
4, 26 -- To: microscopy-at-microscopy.com
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Dear Listservers,

The Penn Regional Nanotechnology Facility at the University of
Pennsylvania has an opening for a Research Scientist/Microscopist. The
facility is equipped with transmission and scanning electron microscopes,
atomic force microscopes, an ion accelerator and a focused ion beam. The
successful candidate will assist the facility's technical director in
training and assisting users, developing methods and maintaining the
instruments.
Qualifications for this position include an M.S. in materials
or other physical science (Ph.D. preferred). A minimum of three years
experience in the operation of SEM, TEM, FIB or AFM (experience with FIB or
AFM preferred).

For immediate consideration, send resume/cv to:

Doug Yates
dmyates-at-lrsm.upenn.edu
Penn Regional Nanotechnology Facility
University of Pennsylvania
3231 Walnut Street
Philadelphia, PA 19104

Additional information about the position may be viewed at:
http://www.hr.upenn.edu/jobs
reference number: 050818006

Information about the Penn Regional Nanotechnology Facility may be viewed at:
http://www.seas.upenn.edu/nanotechfacility

Thanks,
Doug Yates

==============================Original Headers==============================
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7, 28 -- Date: Fri, 5 Aug 2005 16:58:18 -0400
7, 28 -- From: dmyates-at-seas.upenn.edu
7, 28 -- To: Microscopy-at-microscopy.com
7, 28 -- Subject: Microscopist Position Available - Univ Penn
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==============================Original Headers==============================
2, 21 -- From schenderson-at-vcu.edu Fri Aug 5 17:59:29 2005
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2, 21 -- Reply-To: {schenderson-at-vcu.edu}
2, 21 -- From: "Scott Henderson" {schenderson-at-vcu.edu}
2, 21 -- To: {Microscopy-at-microscopy.com}
2, 21 -- Subject: Microscopy Technician position available
2, 21 -- Date: Fri, 5 Aug 2005 18:59:35 -0400
2, 21 -- Organization: Virginia Commonwealth University
2, 21 -- MIME-Version: 1.0
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The following technical position is available at Virginia Commonwealth
University School of Medicine.

Microscopy Technician (Position # 55122) �

A technical position is available in the Microscopy Facility of the
Department of Anatomy and Neurobiology in the School of Medicine at Virginia
Commonwealth University. The facility houses confocal, multi-photon,
fluorescence, and electron microscopes (TEM & SEM).� The successful
candidate will assist with microscopy studies of various biological
systems.� Duties include instructing and assisting users of the facility,
sample preparation, image analysis and minor equipment maintenance.�
Applicants should have excellent communication and organizational skills, an
understanding of basic laboratory procedures, and the ability to manage a
large and varied workload. Qualifications include a degree in Biology/Life
Sciences, at least 2 years of experience with laser scanning microscopy
(confocal and/or multi-photon), sample preparation, digital imaging, and
image analysis.� Experience with electron microscopy is an asset. Computer
skills are essential.�

Applications are to be submitted online via the VCU Jobs website at:

www.vcujobs.com

Click on the �Search Postings� link and under the �Working Title� field,
select �Microscopy Technician�

--------------------------

Scott Henderson, Ph.D.
Director of Microscopy
Associate Professor
Dept. Anatomy & Neurobiology
Virginia Commonwealth University
School of Medicine
Sanger Hall, Rm. 9-069d
1101 East Marshall St.
Richmond, VA 23298-0709

==============================Original Headers==============================
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9, 22 -- Reply-To: {schenderson-at-vcu.edu}
9, 22 -- From: "Scott Henderson" {schenderson-at-vcu.edu}
9, 22 -- To: {Microscopy-at-microscopy.com}
9, 22 -- Subject: Microscopy Technician position available
9, 22 -- Date: Fri, 5 Aug 2005 19:08:06 -0400
9, 22 -- Organization: Virginia Commonwealth University
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ken.blight-at-cancer.org.uk) from
http://microscopy.com/MLFormMail.html on Wednesday, August 3, 2005 at
10:40:02
---------------------------------------------------------------------------

Email: ken.blight-at-cancer.org.uk
Name: Ken Blight

Organization: cancer research uk

Title-Subject: [Filtered] Drosophila eye ultrathin cryosections

Question: I have processed Drosophila eyes for immunolabelling (EM)
using the Tokuyasu technique and the results are, to say the least,
very disappointing. The eyes floated in the fixative which didn't
help matters. The problem looks to be spacial when I compare the
results to standard resin TEM preparation. Maybe there is some
problem with buffers, fixation or section expansion. Does anyone
have experience of these tough little beasties?

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==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:33:05 2005
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6, 12 -- Date: Fri, 5 Aug 2005 19:33:05 -0500
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: ken.blight-at-cancer.org.uk (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Drosophila eye ultrathin cryosections
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cornheadorama-at-hotmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, August 3, 2005 at 18:07:30
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver: Re: Cambridge S250

Question: Hi,

I'm not too familiar with these despite the fact I have two of them
gathering dust. But I have heard them disfavorably compared to the
S200. Mine may be site-rigged for handling semi wafers (and there's a
chance both came from the same facility, at different times... a
hunch) but in mine the stage uses little more substantial than pvc
tubing to transmit the z-axis knob to the stage: horrible hysteresis.

I suspect these and related scopes were enormously popular, and I
know for a fact this 'scope has die-hard fans.

LaB6 is typical but it wouldn't surprise me if there was an active
aftermarket upgrade to FE: indeed one of mine may have one. On the
other hand, you may have missed the bulk of the aftermarket boat by
about 5 years: lots of parts for old scopes falling off of price
lists.... but sometimes onto "clearance" lists :)

But I'd say even if your own scope leaves a lot to be desired,
trading it for another old scope like this? I'd say deosn't quite
make sense unless you have a special requirement. Ultimately, it just
may not be worth the shipping: which of course is dicey unless
there's at least a few hours teardown and re-assembly. Better the
devil you know.

I hope more people respond, I've been back and forth as to whether to
try to get one or both of mine working for about 6 years now :)

-Jeff

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==============================Original Headers==============================
13, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:33:27 2005
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cornheadorama-at-hotmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, August 3, 2005 at 18:41:52
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver:b Re: Osmium Vapors

Question: I don't have experience with osmium compounds, and my
suggestion may not be very practical (read: expensive) unless you
have piles of vacuum fittings lying around like I do, but on occasion
when wanting to "absolutely" seal something up I've used a KF50
stainless nipple (or bellows, sometimes)of appropriate length, a pair
of stainless blanks, clamps, o-rings etc. so that the item could be
sealed "vacuum tight". Of course it's all relative but I wouldn't
think much vapour will diffuse though the ~5mm of highly compressed
Viton. The clamps are a bit clumsy to put on but very quick to come
off: I'm not sure you can get a quick-release clamp in KF50 but that
would make it almost one-handed. You could probably save money and
thru-gassing by having a single-ended arrangment, ie: a pipe section
with a KF50 flange welded on one end and a cap welded on the other.
Some (if not most) of the hi-vac houses weld their standard catalog
items on-site, and some don't charge much extra (~%20 premium) for
custom items from stock parts.

I think the idea of absorbants/reactants holds a lot of potential.
Activated charcoal comes immediately to mind.
Is it so reactive as to present a fire hazard if quickly adsorbed
onto the huge surface area?

-Jeff

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==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:33:55 2005
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8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Osmium Vapors
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (omelon-at-geology.utoronto.ca) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Thursday, August 4, 2005 at 16:57:53
---------------------------------------------------------------------------

Email: omelon-at-geology.utoronto.ca
Name: Christopher R. Omelon

Organization: University of Toronto

Title-Subject: [Filtered] further hardening of LR White

Question: I have tried embedding large samples (~ 1cm3) of porous
rock material in a variety of resins and find that LR White (Hard)
provides the best infilling due to its low viscosity. That being
said, the blocks are not as hard as when using epoxide resins such as
EMBED 812. I am cold-curing (i.e. with accelerator) at both room
temperature and at 4?C with mixed results, but even in the best case
the blocks are not as hard as I would expect. My question: can I
further harden the blocks by now placing these samples in the oven at
60?C? My reasoning is that resin within the block (i.e. beneath the
surface and therefore not exposed to oxygen) will proceed with
polymerization at a faster rate than if I left the blocks at room
temperature. Or is the reaction now at completion? Thanks

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==============================Original Headers==============================
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: omelon-at-geology.utoronto.ca (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: further hardening of LR White
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (block-at-nova.edu) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
August 5, 2005 at 09:44:34
---------------------------------------------------------------------------

Email: block-at-nova.edu
Name: R. Block

Organization: NSU

Title-Subject: [Filtered] MListserver:

Question: Can you give me a source of information explaining pseudo-
confocal microscopy, how the data are acquired and analyzed?
Thanks in advance.

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:35:01 2005
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: block-at-nova.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: pseudo-confocal microscopy
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (elke.buschbeck-at-uc.edu) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, August 5, 2005 at 12:04:08
---------------------------------------------------------------------------

Email: elke.buschbeck-at-uc.edu
Name: Elke

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:Sodium Azide and TEM

Question: Does anyone know if it is o.k. to use sodium azide with TEM
preps? Does it wash out o.k. or will it leave a nasty deposit?
Thanks

---------------------------------------------------------------------------

==============================Original Headers==============================
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6, 12 -- From: elke.buschbeck-at-uc.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Sodium Azide and TEM
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sdmegs09-at-yahoo.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
August 5, 2005 at 13:04:14
---------------------------------------------------------------------------

Email: sdmegs09-at-yahoo.com
Name: Meghan Donahue

Organization: University of San Diego

Title-Subject: [Filtered] MListserver: Energy dispersive spectroscopy fixation

Question: I am trying to fix plant material for EDS in the TEM and I
cannot find literature stating the fixation techniques. Is fixation
different for EDS?

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:35:46 2005
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: sdmegs09-at-yahoo.com (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Energy dispersive spectroscopy fixation
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lauriern-at-videotron.ca) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 2, 2005 at 18:43:47
---------------------------------------------------------------------------

Email: lauriern-at-videotron.ca
Name: Norm

Organization: None

Education: Graduate College

Location: Canada

Question: What is the maximum resolution achievable in Phase Contrast
and also Interferential Contrat DIC microscopy.
Since in bright field it is appr 1.40 I am sure that because of the
phase rings as well as the use of Wollaston prism in DIC, it must be
much lower even using oil immersion objective.

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:36:15 2005
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8, 12 -- From: lauriern-at-videotron.ca (by way of Ask-A-Microscopist)
8, 12 -- Subject: AskAMicroscopist: maximum resolution achievable in Phase Contrast
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Elka,
We use it routinely in buffers used to make up solutions for
immunolocalization on sections. I have never had a problem with
percipitation from sodium azide.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 8/5/05 7:41 PM, "elke.buschbeck-at-uc.edu" {elke.buschbeck-at-uc.edu} wrote:

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} Email: elke.buschbeck-at-uc.edu
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}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
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One concern I have always heard was the formation of metal azide,
especially lead azide. These explosive and unstable have been found in
older metal plumbing. When I started as a QC chemist years ago, dilute
concentrations of sodium azoide was used as a perservative in some test
solutions used in clinical chemistry. One than one person got an
unpleasent surprize when working with the older style lead drain pipes. I
later wanted to use a test solution for sulfur which contained sodium
azide, my management had fits. I never did use that test.

I don't want to creat an electron storm of words, but I will suggest you
check out disposial method for your own safety.

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

330-668-2235 Ext. 238

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elke.buschbeck-at-uc
.edu To: frank.karl-at-degussa.com
cc:
08/05/2005 08:38 Subject: [Microscopy] viaWWW: Sodium Azide and TEM
PM
Please respond to
microscopy

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Title-Subject: [Filtered] MListserver:Sodium Azide and TEM

Question: Does anyone know if it is o.k. to use sodium azide with TEM
preps? Does it wash out o.k. or will it leave a nasty deposit?
Thanks

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Dear Microscopists,

We are looking to hire an Associate Scientist in our Bioimaging Center.
Although we place special emphasis on scanning probe microscopy, skills
in other areas of microscopy would be highly desirable. Please see the
job announcement below for additional details.

Best Regards, Kirk

*Associate Scientist � Bioimaging*

*University of Delaware*

*Delaware Biotechnology Institute*

The Delaware Biotechnology Institute (DBI) was established in 1999 as an
academic unit of the University of Delaware to position Delaware as a
leader in the life sciences. The Institute�s mission is to engage in
leading-edge scientific discovery in the life sciences, provide
biotechnology-based education, and promote economic development. The
major interdisciplinary research areas include human health,
agriculture, marine ecosystems and biomaterials.

The Institute functions as a partnership involving State government, the
Delaware institutions of higher education, and area industry, and is
housed in a new 72,000 ft2 state-of-the-art research facility. In
addition to individual research laboratories, it houses several core
facilities including a custom microarray center, a bioinformatics center
and a bioimaging center. The bioimaging center provides an array of
microscopy expertise and equipment, including conventional fluorescence,
confocal, multiphoton, atomic force, laser microdissection, transmission
and field emission scanning microscopes and ancillary sample preparation
equipment.

Under the limited direction of the Director of the Bioimaging Center,
the Associate Scientist independently interprets, organizes, executes,
and coordinates research assignments. Formulates and conducts research
on problems of considerable scope and complexity.

MAJOR RESPONSIBILITIES:

Provide consultation, training and supervision of graduate students,
post-doctoral fellows, staff and faculty in the design, implementation
and execution of microscopy-related projects with special emphasis on
scanning probe microscopy.

Interpret, organize, execute, and coordinate scientific research
assignments concerned with unique and highly complex problems.

Develop competitive research proposals to successfully generate external
research funding.

Collaborate with users and principal investigators on design, analysis,
application, and reporting of research projects; teach and advise on
techniques.

Design, perform, and/or oversee experiments, collect, analyze, and
interpret data, ensure data integrity, quality control, and protocol
compliance, prepare statistical and narrative reports and/or graphs.

Perform research assignments involving a number of variables, apply
diversified knowledge of scientific research principles, practices, and
protocols in research projects; make recommendations and conclusions
which serve as the basis for decision making.

Make authoritative decisions and recommendations that have a major
impact on scientific research activities and result in national and/or
international recognition.

Evaluate, select, and apply standard scientific techniques, procedures,
and criteria to accomplish a variety of research assignments.

Maintain a broad knowledge of state-of-the-art technology, software,
and/or systems.

Perform miscellaneous job-related duties as assigned

QUALIFICATIONS:

Master�s degree, Ph.D. preferred, in Biology/Chemistry or related field
as well as a record of peer reviewed publications and at least 5 years
demonstrated experience in scanning probe microscopy. Knowledge of
scientific approach, methodologies and scientific research principles,
practices and protocols in order to design, organize and coordinate
scientific research projects. Ability to perform independent original
research in an advanced area of scientific expertise. Ability to develop
scientific reports, proposals and publications on original research and
a knowledge of contemporary technological developments in the area of
scanning probe microscopy. Knowledge of the use and maintenance of
laboratory facilities and/or equipment. Ability to use independent
judgment to adapt and modify research concepts and approaches to
specific projects. Knowledge of current technological
developments/trends in area of scanning probe microscopy. Strong
communication, personal and organizational skills. Motivation to
learn/develop new techniques, flexibility, and ability to interact with
a diverse group of research personnel. approaches to specific projects.
Knowledge of current technological developments/trends in area of
scanning probe microscopy. Strong communication, personal and
organizational skills. Motivation to learn/develop new techniques,
flexibility, and ability to interact with a diverse group of research
personnel.

*Contact:* Interested candidates should forward curriculum vitae and the
names and contact information for three references to Dr. Kirk Czymmek,
15 Innovation Way, Suite 117, Delaware Biotechnology Institute,
University of Delaware, Newark, DE 19711 or by email (kirk-at-udel.edu).

Details can also be found at the following website:
http://www.dbi.udel.edu/career.html

==============================Original Headers==============================
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On Aug 5, 2005, at 5:36 PM, sdmegs09-at-yahoo.com wrote:

} Question: I am trying to fix plant material for EDS in the TEM and I
} cannot find literature stating the fixation techniques. Is fixation
} different for EDS?
}
Dear Meghan,
The preparation methods for any sample on which you are doing EDS or
other analytic techniques must take into account the elements you are
trying to analyze, so, for example, if you are looking for
water-soluble elements like Na or Cl ion, do not rinse the tissue in
solvents that will remove them. Note, however, that if you are looking
for Cl bound in organic compounds, such as polychlorinated biphenyls,
washing out the ion will enable you to distinguish the organochlorine.
For accurate quantitation, it is a good idea, if possible, to examine
the material untreated, except for freezing and perhaps sectioning,
then dehydrate by lyophylization in the scope and redo the analysis.
This will give accurate values for volatile elements and you can
improve the quantitation by taking the wet/dry ratios from elements
that are not affected by the drying process. Another consideration,
for the case that the elements of interest are not affected by your
preparation process, is not to use a stain that interferes with lines
from those elements. Pb and S are notorious for this, and one case I
ran into was trying to analyze Pt and Ir in a specimen that was treated
with Os and given to me. Since your specimens may not be suitable for
observation in the TEM when not prepared in some way, and since you may
not have access to cryopreparation methods, you need to keep in mind
the general principles that, whatever the preparation steps, they
cannot affect the concentration of the elements of interest, and cannot
interfere with the lines you want to measure. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

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A few weeks ago someone asked about a beam stop for a TEM.

Sopme time ago I designed a combination beam stop and Faraday cup for
a JEOL 2010 TEM. It has been in use for several years now, and I
understand has performed satisfactorily. If anyone is interested in
making use of the design, contact me.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-662-5237
Address mail to: 1136 Mixtwood Rd.
Ann Arbor, MI 48103-3035

==============================Original Headers==============================
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3, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu}
3, 14 -- Subject: [Microscopy]TEM: RE: Beam stop & Faraday cup
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and a terrific boss!
:)

----- Original Message -----
X-from: schenderson-at-vcu.edu

Dr. Bigelow,
I would love to have use of the design for our 2010. Also, do you think it
could be altered to fit a 1200 EX-II?

-----Original Message-----
X-from: bigelow-at-engin.umich.edu [mailto:bigelow-at-engin.umich.edu]
Sent: Monday, August 08, 2005 11:58 AM
To: jfb-at-uidaho.edu

A few weeks ago someone asked about a beam stop for a TEM.

Sopme time ago I designed a combination beam stop and Faraday cup for
a JEOL 2010 TEM. It has been in use for several years now, and I
understand has performed satisfactorily. If anyone is interested in
making use of the design, contact me.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-662-5237
Address mail to: 1136 Mixtwood Rd.
Ann Arbor, MI 48103-3035

==============================Original Headers==============================
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Hi:

A guy came into the lab today asking me to image and measure some holes in
a silicon nitride wafer.

OK, I say, how big?

2 nm he says, some maybe up to 70 nm.

That's pretty small, I said. How did you plan on doing it?

Don't know, that's why I came to you, says he.

Here is what he's got. A chip about 1 mm thick with an FIB section out of
it that is like an inverted pyramid into the chip. The pyramid doesn't go
all the way through, but there is a thin area at the bottom that is
supposed to have the tiny hole. The chip is opaque in the TEM, way too
thick, the thin area is translucent at 80KV so we can sort of see where we
are going.

We rigged up a special TEM holder so we could put the chip in the TEM, but
searching for the FIB hole, then the tiny hole inside that took a while.
Eventually I could see something that looked like a hole and it was about
100 nm across. He said that was OK, it was a test hole and should be about
100 nm.

Finding this 100 nm hole was no picnic and I anticipate finding and
measuring a 2 nm hole to be harder. He wants to be able to pop one in and
check the diameter and pop in another one.

Anyone with some bright ideas about how to help this guy? We brainstormed
things like FESEM, AFM, some kind of diffraction with lasers, don't know if
any of them will work, don't have any more ideas.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu

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I've seen it as a constituent of a TEM fixative, so it shouldn't be a
problem. But why bother?

Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
}
}
} Email: elke.buschbeck-at-uc.edu
} Name: Elke
}
} Organization: University of Cincinnati
}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
}

==============================Original Headers==============================
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Any idea if there will be a single hole per sample or multiple holes? Will
you have any idea what the probable depth of the small hole will be (not
including the thinning they are apparently doing to the substrate)? The
hole's aspect ratio will have a lot to do with any analysis since a large
ratio (small diameter hole, long bore) can make alignment with any imaging
system a big problem.

This is probably a good time to jump in and see if they can't adjust their
experiment a little. Too often researchers jump before they can walk, and
assume that an existing method can provide whatever information they need,
without providing any compliance to existing techniques. A thinner
substrate would require less preliminary FIB thinning on their part and if
it were thin enough, could allow you to determine and measure the presence
of a hole even if the bore weren't perfectly aligned with the beam.

While they may be reluctant to discuss the work they are doing, details
aren't really needed. It may be in their best interest to understand the
hole formation process before extending it to such a thick substrate that
requires multiple operations. Unfortunately, it may be up to you to point
that out.

Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
Saint Charles, Illinois 60174
phone (630) 513-7093 fax (630) 513-7092
email mailto:ars-at-sem.com web www.sem.com

On Monday, August 08, 2005 5:51 PM, jmkrupp-at-cats.ucsc.edu
[SMTP:jmkrupp-at-cats.ucsc.edu] wrote:
}
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}
} Hi:
}
} A guy came into the lab today asking me to image and measure some holes
in
} a silicon nitride wafer.
}
} OK, I say, how big?
}
} 2 nm he says, some maybe up to 70 nm.
}
} That's pretty small, I said. How did you plan on doing it?
}
} Don't know, that's why I came to you, says he.
}
} Here is what he's got. A chip about 1 mm thick with an FIB section out of
} it that is like an inverted pyramid into the chip. The pyramid doesn't go
} all the way through, but there is a thin area at the bottom that is
} supposed to have the tiny hole. The chip is opaque in the TEM, way too
} thick, the thin area is translucent at 80KV so we can sort of see where
we
} are going.
}
} We rigged up a special TEM holder so we could put the chip in the TEM,
but
} searching for the FIB hole, then the tiny hole inside that took a while.
} Eventually I could see something that looked like a hole and it was about
} 100 nm across. He said that was OK, it was a test hole and should be
about
} 100 nm.
}
} Finding this 100 nm hole was no picnic and I anticipate finding and
} measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} check the diameter and pop in another one.
}
} Anyone with some bright ideas about how to help this guy? We brainstormed
} things like FESEM, AFM, some kind of diffraction with lasers, don't know
if
} any of them will work, don't have any more ideas.
}
} Thanks
}
} Jon
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
} ==============================Original
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==============================Original Headers==============================
7, 20 -- From ars-at-sem.com Tue Aug 9 00:20:36 2005
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I don't know your exact problem, but some names of people who have
worked with tissues and elemental analysis:

P. Echlin
H. Plattner
A. Somlyo
RA Steinbrecht and K. Zierold

There are many more - sorry for not mentioning these names.

best regards,
RR

-------------------------------
PD Dr.Reinhard Rachel
Universit�t Regensburg
Lehrstuhl f�r Mikrobiologie
Universitaetsstr. 31
D-93053 Regensburg
tel.: +49 941 943 4534
fax.: +49 941 943 1824

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I will be in Peru from the 11th Aug to 20 Aug.
I will have a delayed time acces to emails.
For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
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==============================Original Headers==============================
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==============================Original Headers==============================
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I don't care
Chris

----- Original Message -----
X-from: {luc-at-anaspec.co.za}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Tuesday, August 09, 2005 8:22 AM

I will be in Peru from the 11th Aug to 20 Aug.
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Hi George
Did you ever get the schematics for the ISI 100B that you were looking for?
Cheers
Peter Earl
Toronto

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2, 26 -- From: "Peter Earl" {pwje-at-sympatico.ca}
2, 26 -- To: {microscopy-at-microscopy.com}
2, 26 -- References: {200507180006.j6I066ku023229-at-ns.microscopy.com}
2, 26 -- Subject: Re: [Microscopy] AskAMicroscopist: schematics for an International Scientific
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Dear Dr.Henderson,

I noticed with interest your recent employment note on the Microscopy
listserver and hope that you are having good success.

Our publication, Microscopy Today, is mailed six times per year to
nearly 15,000 microscopists -- each of whom has either specifically
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Regards,

Ron Anderson, Editor
Microscopy Today

schenderson-at-vcu.edu wrote:

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13, 20 -- From randerson20-at-tampabay.rr.com Tue Aug 9 08:43:17 2005
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Why use sodium azide?

We are planning to use sodium azide for the first time as we wish to
store SEM samples for 3-4 months (rare samples available now for a
student project in November). I have found that very occasionally
something can grow in sodium cacodylate buffer. I think there are some
fungi that like it :)

Dave

-----Original Message-----
X-from: dianavd-at-eye.usyd.edu.au [mailto:dianavd-at-eye.usyd.edu.au]
Sent: 09 August 2005 02:11
To: David Patton

I've seen it as a constituent of a TEM fixative, so it shouldn't be a
problem. But why bother?

Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
}
}
} Email: elke.buschbeck-at-uc.edu
} Name: Elke
}
} Organization: University of Cincinnati
}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
}

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19, 32 -- From David.Patton-at-uwe.ac.uk Tue Aug 9 09:21:45 2005
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1. Is my face red! Only yesterday I read that with the new listserver
setup that hitting reply--intending to send mail to the person who
originated the initial email only--in fact sends the reply to the whole
listserver! Please disregard the email to Henderson. I'll try my best
not to make that mistake again! I'm sorry.

2. To the folks who sent me nasty, nearly obscene diatribes castigating
me for this simple mistake: please try and get a grip on life. Perhaps a
nice nap will help.

Ron Anderson
Microscopy Today

==============================Original Headers==============================
6, 20 -- From randerson20-at-tampabay.rr.com Tue Aug 9 09:34:23 2005
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one can only wonder if these same people have time to
do anything else beside criticize others.
i have been on the receiving end of these diatribes.
and yes people do need to get a life.
john
ps and yes i meant to send this to the whole
listserver

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} 1. Is my face red! Only yesterday I read that with
} the new listserver
} setup that hitting reply--intending to send mail to
} the person who
} originated the initial email only--in fact sends the
} reply to the whole
} listserver! Please disregard the email to
} Henderson. I'll try my best
} not to make that mistake again! I'm sorry.
}
} 2. To the folks who sent me nasty, nearly obscene
} diatribes castigating
} me for this simple mistake: please try and get a
} grip on life. Perhaps a
} nice nap will help.
}
} Ron Anderson
} Microscopy Today
}
}
}
}
} ==============================Original
} Headers==============================
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} 09:34:23 2005
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} from MT!
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5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 9 10:19:16 2005
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i didn't know the listserver was offering home loans.

--- ypuizaluzec-at-microscopy.com wrote:

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} Hello
}
} Homeowner
}
} You have been pre-approved for a $402,974 Home Loan
} at a 4.53% Fixed Rate.
} This offer is being extended to you unconditionally
} and your credit is in no way a factor.
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} To take Advantage of this Limited Time opportunity
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} All we ask is that you visit our Website and
} complete
} The 1 minute post Approval Form
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} http://mrYC3sFjAhXM0.loanvoice.com/?name=rnnn
}
} Have a Good Day,
}
} Nelle Kendall
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} 08:07:44 2005
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On 8/9/05 12:24 AM, "ars-at-sem.com" {ars-at-sem.com} wrote:

}
}
}
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}
} Any idea if there will be a single hole per sample or multiple holes? Will
} you have any idea what the probable depth of the small hole will be (not
} including the thinning they are apparently doing to the substrate)? The
} hole's aspect ratio will have a lot to do with any analysis since a large
} ratio (small diameter hole, long bore) can make alignment with any imaging
} system a big problem.
}
} This is probably a good time to jump in and see if they can't adjust their
} experiment a little. Too often researchers jump before they can walk, and
} assume that an existing method can provide whatever information they need,
} without providing any compliance to existing techniques. A thinner
} substrate would require less preliminary FIB thinning on their part and if
} it were thin enough, could allow you to determine and measure the presence
} of a hole even if the bore weren't perfectly aligned with the beam.
}
} While they may be reluctant to discuss the work they are doing, details
} aren't really needed. It may be in their best interest to understand the
} hole formation process before extending it to such a thick substrate that
} requires multiple operations. Unfortunately, it may be up to you to point
} that out.
}
}
} Allen R. Sampson, Owner
} Advanced Research Systems
} 317 North 4th. Street
} Saint Charles, Illinois 60174
} phone (630) 513-7093 fax (630) 513-7092
} email mailto:ars-at-sem.com web www.sem.com
}
}
} On Monday, August 08, 2005 5:51 PM, jmkrupp-at-cats.ucsc.edu
} [SMTP:jmkrupp-at-cats.ucsc.edu] wrote:
} }
} }
} }
} }
} ------------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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} }
} } Hi:
} }
} } A guy came into the lab today asking me to image and measure some holes
} in
} } a silicon nitride wafer.
} }
} } OK, I say, how big?
} }
} } 2 nm he says, some maybe up to 70 nm.
} }
} } That's pretty small, I said. How did you plan on doing it?
} }
} } Don't know, that's why I came to you, says he.
} }
} } Here is what he's got. A chip about 1 mm thick with an FIB section out of
} } it that is like an inverted pyramid into the chip. The pyramid doesn't go
} } all the way through, but there is a thin area at the bottom that is
} } supposed to have the tiny hole. The chip is opaque in the TEM, way too
} } thick, the thin area is translucent at 80KV so we can sort of see where
} we
} } are going.
} }
} } We rigged up a special TEM holder so we could put the chip in the TEM,
} but
} } searching for the FIB hole, then the tiny hole inside that took a while.
} } Eventually I could see something that looked like a hole and it was about
} } 100 nm across. He said that was OK, it was a test hole and should be
} about
} } 100 nm.
} }
} } Finding this 100 nm hole was no picnic and I anticipate finding and
} } measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} } check the diameter and pop in another one.
} }
} } Anyone with some bright ideas about how to help this guy? We brainstormed
} } things like FESEM, AFM, some kind of diffraction with lasers, don't know
} if
} } any of them will work, don't have any more ideas.
} }
} } Thanks
} }
} } Jon
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
} }
} }
} }
} } ==============================Original
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} } 15, 17 -- To: microscopy-at-microscopy.com
} } 15, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
} } 15, 17 -- Subject: Looking for tiny holes
} } 15, 17 -- X-UCSC-CATS-MailScanner: Found to be clean
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} ==============================Original Headers==============================
} 7, 20 -- From ars-at-sem.com Tue Aug 9 00:20:36 2005
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} 7, 20 -- Date: Tue, 9 Aug 2005 00:20:29 -0500
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}
} The thickness at a hole depends upon whether a precipitate fell out, which
leaves a thicker region around a hole. If a hole is formed by electropolishing
an annealled sample of a pure metal, or even some alloys, the area around the
hole may be very thin. 20 to 50 nanometers might be typical thicknesses
achieved.
Bernie Kestel
Argonne National Lab E-mail: kestel-at-anl.gov

==============================Original Headers==============================
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Sandy,
I saw that yesterday. I guess things are working out for Scott, if
his lab is growing. That's good.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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Colleagues;

I believe I have plugged the loop-hole that allowed the Housing
for Sale Email to get through the system earlier today. It was
a unique situation to say the least, but then again they are all
getting this way.

At the same time since I was editing the code I have also changed the
Listserver Software so that a reply should nolonger go automatically
to the Listserver but to the sender.

Nevertheless I caution you to please always look at what your sending.
It only takes a moment.

If you wish to send a copy of your "reply" to a message to the Listserver
you will now have to explicitly add the Listserver Email
address (microscopy-at-microscopy.com ) to your recepients list

Nestor
Your Friendly Neighborhood SysOp

--

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Sodium azide should be used very carefully. It has been used as a biological
preservative for a long time. In 1968 I was working in a CDC lab in Phoenix
(Yes, there was a CDC branch there at that time) where we used sodium azide to
preserve hepatitis antigens. One of the technicians was using an aspiration
tube attached to a pipette to transfer sodium azide solution to an antigen
preparation. She coughed and sucked the solution into her lungs. She was on
the floor gasping for breath within 15 seconds. There was an MD sitting in
his office next to the technician's lab. He heard her fall to the floor. He
new what she was doing and determined what had happened. He had what he
called a "universal antitdote" in his office. He administered the antidote
and gave her mouth-to-mouth resuscitation and she survived.

I know (I hope!!) people don't use aspiration tubes today but this incident
sure brought home to me how how quickly sodium azide works. You may want to
look at the following CDC URL:
http://www.bt.cdc.gov/agent/sodiumazide/basics/facts.asp Although
this article states that there is no specific antidote for sodium azide
poisoning, in the incident I related above, an antidote was given. I don't
remember what it was.

} ----------
} From: dianavd-at-eye.usyd.edu.au
} Reply To: microscopy-at-microscopy.com
} Sent: Monday, August 8, 2005 6:11 PM
} To: John Wheatley
} Subject: [Microscopy] viaWWW: Sodium Azide and TEM
}
}
}
}
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} I've seen it as a constituent of a TEM fixative, so it shouldn't be a
} problem. But why bother?
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
} }
} }
} } Email: elke.buschbeck-at-uc.edu
} } Name: Elke
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
} }
} } Question: Does anyone know if it is o.k. to use sodium azide with TEM
} } preps? Does it wash out o.k. or will it leave a nasty deposit?
} } Thanks
} }
}
}
} ==============================Original Headers==============================
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Sodium azide should be used very carefully. It has been used as a
biological preservative for a long time. In 1968 I was working in a
CDC lab in Phoenix (Yes, there was a CDC branch there at that time)
where we used sodium azide to preserve hepatitis antigens. One of
the technicians was using an aspiration tube attached to a pipette to
transfer sodium azide solution to an antigen preparation. She
coughed and sucked the solution into her lungs. She was on the floor
gasping for breath within 15 seconds. There was an MD sitting in
his office next to the technician's lab. He heard her fall to the
floor. He new what she was doing and determined what had happened.
He had what he called a "universal antitdote" in his office. He
administered the antidote and gave her mouth-to-mouth resuscitation
and she survived.

I know (I hope!!) people don't use aspiration tubes today but this
incident sure brought home to me how how quickly sodium azide works.
You may want to look at the following CDC URL:
http://www.bt.cdc.gov/agent/sodiumazide/basics/facts.asp
Although this article states that there is no specific antidote for
sodium azide poisoning, in the incident I related above, an antidote
was given. I don't remember what it was.

} ----------
} From: dianavd-at-eye.usyd.edu.au
} Reply To: microscopy-at-microscopy.com
} Sent: Monday, August 8, 2005 6:11 PM
} To: John Wheatley
} Subject: [Microscopy] viaWWW: Sodium Azide and TEM
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I've seen it as a constituent of a TEM fixative, so it shouldn't be a
} problem. But why bother?
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
} }
} }
} } Email: elke.buschbeck-at-uc.edu
} } Name: Elke
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
} }
} } Question: Does anyone know if it is o.k. to use sodium azide with TEM
} } preps? Does it wash out o.k. or will it leave a nasty deposit?
} } Thanks
} }
}
}
} ==============================Original
} Headers==============================
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} 5, 20 -- Received: from galen.med.usyd.edu.au
} (machaon.med.usyd.edu.au [129.78.36.30])
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7, 26 -- From JOHN.WHEATLEY-at-asu.edu Tue Aug 9 12:32:58 2005
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We use a standard SEM to work with nanometer-scale features (~50 nm) in
silicon
nitride. You might consider using electron diffraction. If using a 10
keV beam,
(wavelength of .12 angstroms), a circular aperture with a diameter of 2
nm would
create a circular diffraction pattern (described by an Airy disk). With these
parameters, the radius of the first zero is given by R = 0.007*(distance from
the aperture).

Dunno much about TEM and standard detectors used, but if you have an electron
detector with a spatial resolution of 10 microns placed 1 cm below the sample,
you would be able to resolve the central diffraction order (thereby measuring
the hole diameter). To do this, you'd turn off the scanning mechanism for the
microscope, and you'd want to focus quite a bit beyond the hole itself (to
ensure the electron wavefronts extend across the diameter of the hole.

Anyway, I'm sure there are more straightforward solutions, but this is just an
idea. We do similar stuff all the time.

--
Ben McMorran
Research Assistant, Atom Optics Group
Department of Physics
University of Arizona
1118 E 4th St
Tucson, AZ
USA

ph. 520-621-2688

Quoting jmkrupp-at-cats.ucsc.edu:

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} Hi:
}
} A guy came into the lab today asking me to image and measure some holes in
} a silicon nitride wafer.
}
} OK, I say, how big?
}
} 2 nm he says, some maybe up to 70 nm.
}
} That's pretty small, I said. How did you plan on doing it?
}
} Don't know, that's why I came to you, says he.
}
} Here is what he's got. A chip about 1 mm thick with an FIB section out of
} it that is like an inverted pyramid into the chip. The pyramid doesn't go
} all the way through, but there is a thin area at the bottom that is
} supposed to have the tiny hole. The chip is opaque in the TEM, way too
} thick, the thin area is translucent at 80KV so we can sort of see where we
} are going.
}
} We rigged up a special TEM holder so we could put the chip in the TEM, but
} searching for the FIB hole, then the tiny hole inside that took a while.
} Eventually I could see something that looked like a hole and it was about
} 100 nm across. He said that was OK, it was a test hole and should be about
} 100 nm.
}
} Finding this 100 nm hole was no picnic and I anticipate finding and
} measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} check the diameter and pop in another one.
}
} Anyone with some bright ideas about how to help this guy? We brainstormed
} things like FESEM, AFM, some kind of diffraction with lasers, don't know if
} any of them will work, don't have any more ideas.
}
} Thanks
}
} Jon
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
} ==============================Original Headers==============================
} 15, 17 -- From jmkrupp-at-cats.ucsc.edu Mon Aug 8 17:48:38 2005
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} 15, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
} 15, 17 -- Subject: Looking for tiny holes
} 15, 17 -- X-UCSC-CATS-MailScanner: Found to be clean
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9, 27 -- From mcmorran-at-physics.arizona.edu Tue Aug 9 14:30:30 2005
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I didn't see that you got a response on this, so I thought that I would
do so.

I built a FIM/IAP for my dissertation work back in the early to mid
80's. Mine was made using UHV stainless steel components. I was
familiar with most of the people in the field at that time and didn't
know anyone that was still putting together glass systems. I think that
it has been a long time since people have been using glass systems for
FIM. When they were, mostly they had their systems custom made by their
in-house glass blowers.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: tttan-at-simtech.a-star.edu.sg [mailto:tttan-at-simtech.a-star.edu.sg]
Sent: Tuesday, August 02, 2005 11:05 AM
To: Walck-at-SouthBayTech.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tttan-at-simtech.a-star.edu.sg) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
August 1, 2005 at 20:24:16
------------------------------------------------------------------------
---

Email: tttan-at-simtech.a-star.edu.sg
Name: TT Tan

Organization: Singapore Inst. of Manuf. Tech.

Title-Subject: [Filtered] Field Ion Microscope

Question: Hi all,

I would like to know from who can I buy the glassware to do field ion
microscopy.

I am looking for a quartz ware that can do the job. Preferably one
that allows me to fit onto a NW40 joint.

Thanks in advance.

------------------------------------------------------------------------
---

==============================Original
Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Tue Aug 2 12:59:37 2005 9, 12 --
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21, 26 -- From walck-at-southbaytech.com Tue Aug 9 22:25:48 2005
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21, 26 -- From: "Scott Walck" {walck-at-southbaytech.com}
21, 26 -- To: {microscopy-at-microscopy.com}
21, 26 -- Subject: RE: [Microscopy] viaWWW: Field Ion Microscope
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (montiorm-at-ucmail.uc.edu) from http://microscopy.com/MLFormMail.html on Thursday, July 28, 2005 at 12:57:07
---------------------------------------------------------------------------

Email: montiorm-at-ucmail.uc.edu
Name: Richard Montione

Organization: University of Cincinnati

Title-Subject: [Filtered] TCS 4D available

Question: RE: TCS 4D

To anyone interested,

I have a Leica TCS 4D confocal system originally installed in 1994. It has a Kr/Ar laser and a water cooled UV laser, with a Leitz DMRBE upright microscope and a number of lenses. It has the usual problems associated with age but was still functional when it was disassembled for storage.

If anyone is interested and would like more information please contact me at montiorm-at-uc.edu

Richard Montione

Electron Microscopy Facility

Department of Pathology

University of Cincinnati

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==============================Original Headers==============================
20, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:26:33 2005
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20, 12 -- From: montiorm-at-ucmail.uc.edu (by way of MicroscopyListserver)
20, 12 -- Subject: viaWWW: TCS 4D available
20, 12 -- Content-Type: text/plain; charset="us-ascii"
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (msimms-at-tracelabs.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, July 29, 2005 at 10:06:10
---------------------------------------------------------------------------

Email: msimms-at-tracelabs.com
Name: Michael Simms

Organization: Trace Laboratories - Central

Title-Subject: [Filtered] Measurement of ink depth/contrast

Question: Hello Gentlemen,
I work for an independent testing organization, Trace Laboratories - Central.
We have been asked to have printing ink measured for depth and contrast. Laser printing is done on the insulation sleeve of a cable.
per Sikorsky specification SS7333, Paragraph 4.6.5
This asks for measuring to an accuracy of 0.0001 inch with a contour projector or calibrated microscope or Zygo measuring device. The contrast measurement is suggested to be made with a Spectrum Technology CMS^2 Contrast Measuring System. I would need evidence of either an accredited laboratory or calibration to pass on to the customer.

Is this something that someone associated with this resource might be able to quote?

Regards,
Mike

Mike Simms
Chemist
Trace Laboratories - Central
1150 W. Euclid Ave.
Palatine, IL 60067

phone 847-934-5300
fax 847-934-4600
www.tracelabs.com

---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:29:45 2005
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9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: msimms-at-tracelabs.com (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: Measurement of ink depth/contrast
9, 12 -- Content-Type: text/plain; charset="us-ascii"
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (stefan.diller-at-t-online.de) from http://microscopy.com/MLFormMail.html on Saturday, July 30, 2005 at 13:43:48
---------------------------------------------------------------------------

Email: stefan.diller-at-t-online.de
Name: Stefan Diller

Title-Subject: [Filtered] Reichert Histostat 8035 Paraffin Embedder manual needed

Question: Dear members,
I am looking urgently for a user manual and if possible a service manual or the electronic layout for the Reichert Histostat Paraffin Embedder Modell 8035.

Best regards,
Stefan Diller

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:31:03 2005
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6, 12 -- From: stefan.diller-at-t-online.de (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Reichert Histostat 8035 Paraffin Embedder manual needed
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ken.blight-at-cancer.org.uk) from http://microscopy.com/MLFormMail.html on Wednesday, August 3, 2005 at 10:40:02
---------------------------------------------------------------------------

Email: ken.blight-at-cancer.org.uk
Name: Ken Blight

Organization: cancer research uk

Title-Subject: [Filtered] Drosophila eye ultrathin cryosections

Question: I have processed Drosophila eyes for immunolabelling (EM) using the Tokuyasu technique and the results are, to say the least, very disappointing. The eyes floated in the fixative which didn't help matters. The problem looks to be spacial when I compare the results to standard resin TEM preparation. Maybe there is some problem with buffers, fixation or section expansion. Does anyone have experience of these tough little beasties?

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==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:32:28 2005
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: ken.blight-at-cancer.org.uk (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Drosophila eye ultrathin cryosections
6, 12 -- Content-Type: text/plain; charset="us-ascii"
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 3, 2005 at 18:07:30
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver: Re: Cambridge S250

Question: Hi,

I'm not too familiar with these despite the fact I have two of them gathering dust. But I have heard them disfavorably compared to the S200. Mine may be site-rigged for handling semi wafers (and there's a chance both came from the same facility, at different times... a hunch) but in mine the stage uses little more substantial than pvc tubing to transmit the z-axis knob to the stage: horrible hysteresis.

I suspect these and related scopes were enormously popular, and I know for a fact this 'scope has die-hard fans.

LaB6 is typical but it wouldn't surprise me if there was an active aftermarket upgrade to FE: indeed one of mine may have one. On the other hand, you may have missed the bulk of the aftermarket boat by about 5 years: lots of parts for old scopes falling off of price lists.... but sometimes onto "clearance" lists :)

But I'd say even if your own scope leaves a lot to be desired, trading it for another old scope like this? I'd say deosn't quite make sense unless you have a special requirement. Ultimately, it just may not be worth the shipping: which of course is dicey unless there's at least a few hours teardown and re-assembly. Better the devil you know.

I hope more people respond, I've been back and forth as to whether to try to get one or both of mine working for about 6 years now :)

-Jeff

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==============================Original Headers==============================
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12, 12 -- To: microscopy-at-microscopy.com
12, 12 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
12, 12 -- Subject: viaWWW: Cambridge S250
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 3, 2005 at 18:41:52
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver:b Re: Osmium Vapors

Question: I don't have experience with osmium compounds, and my suggestion may not be very practical (read: expensive) unless you have piles of vacuum fittings lying around like I do, but on occasion when wanting to "absolutely" seal something up I've used a KF50 stainless nipple (or bellows, sometimes)of appropriate length, a pair of stainless blanks, clamps, o-rings etc. so that the item could be sealed "vacuum tight". Of course it's all relative but I wouldn't think much vapour will diffuse though the ~5mm of highly compressed Viton. The clamps are a bit clumsy to put on but very quick to come off: I'm not sure you can get a quick-release clamp in KF50 but that would make it almost one-handed. You could probably save money and thru-gassing by having a single-ended arrangment, ie: a pipe section with a KF50 flange welded on one end and a cap welded on the other. Some (if not most) of the hi-vac houses weld their standard catalog items on-site, and some don't charge much extra (~%20 premium) for custom items from stock parts.

I think the idea of absorbants/reactants holds a lot of potential. Activated charcoal comes immediately to mind.
Is it so reactive as to present a fire hazard if quickly adsorbed onto the huge surface area?

-Jeff

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==============================Original Headers==============================
9, 14 -- From zaluzec-at-microscopy.com Tue Aug 9 22:34:38 2005
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9, 14 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
9, 14 -- Subject: viaWWW: Re: Osmium Vapors
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (omelon-at-geology.utoronto.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, August 4, 2005 at 16:57:53
---------------------------------------------------------------------------

Email: omelon-at-geology.utoronto.ca
Name: Christopher R. Omelon

Organization: University of Toronto

Title-Subject: [Filtered] further hardening of LR White

Question: I have tried embedding large samples (~ 1cm3) of porous rock material in a variety of resins and find that LR White (Hard) provides the best infilling due to its low viscosity. That being said, the blocks are not as hard as when using epoxide resins such as EMBED 812. I am cold-curing (i.e. with accelerator) at both room temperature and at 4?C with mixed results, but even in the best case the blocks are not as hard as I would expect. My question: can I further harden the blocks by now placing these samples in the oven at 60?C? My reasoning is that resin within the block (i.e. beneath the surface and therefore not exposed to oxygen) will proceed with polymerization at a faster rate than if I left the blocks at room temperature. Or is the reaction now at completion? Thanks

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6, 12 -- From: omelon-at-geology.utoronto.ca (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: further hardening of LR White
6, 12 -- Content-Type: text/plain; charset="us-ascii"
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005 at 12:35:33
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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: We have bags of Kodak Dektol. Does anyone know if I can use this to develop Kodak Em film 4489? At what dilution, for how long?
Thanks for any help.

Stacey Andringa

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Hi All,

We have just had an ergonomist look at our Hitachi S4100 set up as the
primary user is beginning to have some problems which could get very
nasty as time goes on.

Has anyone out there had similar issues? And if so, what issues were
felt to be critical and what solutions were offered?

One of the biggest issues (according to the ergonomist) is the position
of the screens. They are CRT's embedded in a console and as such can't
be moved. Has anyone tried to take the video signals from one of these
systems (or similar) and put them onto a LCD monitor? If so, how was it
done and how successful was it? (these screens are also beginning to
lose their intensity which isn't helping!)

Another issue is the arrangement of the console and column. The
ergonomist feels that they would be better at 90 degrees to each other
but the cable length between the column and console prevents that. Has
anyone tried to lengthen these cables (some go to the HV tank)?

Any help or information would be appreciated.

Cheers

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.

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Since I'm the Manager for the Educational Division I have to put my 2 cents
in.....

NEVER buy a microscope just because it is affordable. I always suggest
getting in a local dealer who can
provide you with a demonstration AND be there after the sale in case you
need service.

There are many "off brand" microscopes out there that are a good way to
through away money. I have been
in many labs where whole sets of microscopes are on the shell because one
or two starting smoking and the
lab manager was afraid to use them. I have also tried servicing some of
these off brands and the materials
used and the machining makes them good ship achors :-)

For your own best interest, call a local dealer and get a demonstration -
it's also good to ask for references from
people who have used the microscope you are looking to buy. Remember the
saying "you get what you pay for".
Bad quality microscopes will prevent you from being able show the students
what you are trying to teach.

If you have any questions, please do not hesitate to contact me.

Gretchen

Gretchen Ziegler, Sales Manager, Educational Division
Leica Microsystems, Inc.
P.O. Box 151 - Ocean Grove, NJ 07756
Phone: 732-897-9506 - Fax: 847-236-3013
Voicemail: 1-800-248-0665 ext. 5131

ballardmark-at-gmail
.com To: Gretchen.Ziegler-at-leica-microsystems.com
cc:
07/28/2005 09:08 Subject: [Microscopy] AskAMicroscopist: an affordable student microscope
PM
Please respond to
microscopy

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Below is the result of your feedback form (NJZFM-ultra-55). It was
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Email: ballardmark-at-gmail.com
Name: Marcello

Organization: None

Education: 9-12th Grade High School

Location: Orlando, Florida

Question: Hi to all,

i am trying to buy the most afforable microscopy, but that will be
able to help me with biology and chemistry.

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Hello Colin,

We have an Hitachi S-4000 which I believe is very similar to the S-4100. We
have encountered both of this issues and have addressed them as follows:

We attempted to turn the column cabinet in order to enable users better
access to the manual stage controls. As you observed the cable length is
insufficient to accomplish more than 20 -30 degree of rotation. We also
determined that turning the column introduced a new problem...it became
difficult to introduce a sample into the SEC port (which is our primary
sample entry port). Our solution was to purchase a motorized stage. While
this was a costly upgrade, it was money well spent. The 5 axis stage we
purchased from a company called E.Fjeld(http://www.efjeld.com/P_5500.htm)has
performed much better that anticipated. As expected wear and tear on the
user is dramatically improved! In addition the stage motion is much more
precise and backlash is nonexistent.

On the S-4000 there was a simple solution to adding a high resolution CRT
type monitor. A BNC port accessing the display crt already existed. If you
open the back lower console panel you should see two BNC ports in the upper
middle region (ours are unlabelled). We connected a cable from the left
port to the B & W monitor. If you have schematics for the system you should
be able to determine if this port exist on your system. Unfortunately I
don't know much about LCD monitors and their cabling options so this could
be an issue when selecting a monitor. However, we have been satisfied with
the B & W CRT. Also, we haven't noticed any adverse field effects while the
CRT monitor is in use.

While not addressed in your post a third area relating to ergonomics was
addressed by elimination of the Polaroid camera system. We purchased a
passive acquisition system from PCI
Quartz(http://www.qrtz.com/acquisition.html) which allows us to capture
digital images.

While the upgrades were costly they have improved the user interface,
increased productivity, and added to the useful lifetime of the instrument.
The system is 16+ years old and continues to produce quality results in a
digital environment. I'm not sure how much, if any, of this information is
helpful or relevant? However, if you have any question please feel free to
contact me directly. Good luck, jr.

Disclaimer: These are my opinions only and do not reflect those of my
employer. While I am a satisfied customer I have no financial ties to any of
these manufacturers. All of the upgrades mentioned are available from
multiple vendors and it is suggest that you investigate all options prior to
making any purchases for your system.

John A. Robson
Boehringer Ingelheim Pharmaceuticals, Inc.
PO Box 368
900 Ridgebury Rd
Ridgefield, CT 06877

Phone (203)798-5640
Fax (203)798-5698
e-mail jrobson-at-RDG.boehringer-ingelheim.com

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That's an easy one.....no! Dektol is for developing photo paper, not
film. Take it from someone who did this by mistake once and got
negatives with grain the size of gravel and films so thick you could use
them to view eclipses. It just might be possible with enough testing
and fooling to get negatives you could actually see through, but the
quality would be pretty awful anyway.

If you don't make prints anymore, donate your Dektol to your local
photography classes.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

-----Original Message-----
X-from: Stacey.Andringa-at-uc.edu [mailto:Stacey.Andringa-at-uc.edu]
Sent: Tuesday, August 09, 2005 10:40 PM
To: Tindall, Randy D.

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Stacey.Andringa-at-uc.edu) from
http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005 at
12:35:33
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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: We have bags of Kodak Dektol. Does anyone know if I can use
this to develop Kodak Em film 4489? At what dilution, for how long?
Thanks for any help.

Stacey Andringa

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Hello All,
Can anyone advise this new user to our lab? His prep/questions are
below. I think that he needs negative staining. How best to prep this
sample?/fixation/washing out the sucrose?? and what type of filmed
grids are being used these days??Formvar/Pioloform??other??

-----------------------

Basically what I have is a 100nm virus particle bound to a 100nm
liposome molecule by a Nickel NTA "bridge". In short, I would like a
picture of this complex. So, If I had this complex (usually in
~10-20%sucrose solution), what would I do from there as far a
preparation?

----------------------
Thanks,
Linda

Linda M. Fox
Loyola University
Stritch School of Medicine
Core Imaging Facility
2160 S. First Ave.
Maywood, Il 60153
Bld. 102 Room 0617
1-708-216-3395
lfox1-at-lumc.edu

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Bill,

Ron has been trying to get me to rejoin MT, so ...
This would make an excellent article, would you be willing to write
it up for MT?
Note, we'd also like articles on cryoTEM and tomography.

Phil

} On Aug 5, 2005, at 5:36 PM, sdmegs09-at-yahoo.com wrote:
}
} } Question: I am trying to fix plant material for EDS in the TEM and I
} } cannot find literature stating the fixation techniques. Is fixation
} } different for EDS?
} }
} Dear Meghan,
} The preparation methods for any sample on which you are doing EDS or
} other analytic techniques must take into account the elements you are
} trying to analyze, so, for example, if you are looking for
} water-soluble elements like Na or Cl ion, do not rinse the tissue in
} solvents that will remove them. Note, however, that if you are looking
} for Cl bound in organic compounds, such as polychlorinated biphenyls,
} washing out the ion will enable you to distinguish the organochlorine.
} For accurate quantitation, it is a good idea, if possible, to examine
} the material untreated, except for freezing and perhaps sectioning,
} then dehydrate by lyophylization in the scope and redo the analysis.
} This will give accurate values for volatile elements and you can
} improve the quantitation by taking the wet/dry ratios from elements
} that are not affected by the drying process. Another consideration,
} for the case that the elements of interest are not affected by your
} preparation process, is not to use a stain that interferes with lines
} from those elements. Pb and S are notorious for this, and one case I
} ran into was trying to analyze Pt and Ir in a specimen that was treated
} with Os and given to me. Since your specimens may not be suitable for
} observation in the TEM when not prepared in some way, and since you may
} not have access to cryopreparation methods, you need to keep in mind
} the general principles that, whatever the preparation steps, they
} cannot affect the concentration of the elements of interest, and cannot
} interfere with the lines you want to measure. Good luck.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
} ==============================Original Headers==============================
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

==============================Original Headers==============================
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Dear Listers,

Has anyone used a low melting point (LMP) agarose to
encapsulate cells for TEM? If yes, which resin is
best suited for LMP agarose encapsulted cells? We
have in the lab Durcupan ACM resin, EMbed-812, LRW
resin kits.

The samples were encapsulated after buffer wash, after
osmium fixation.

Thank you,

Claire

__________________________________________________
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==============================Original Headers==============================
6, 18 -- From lukeclaire-at-yahoo.com Wed Aug 10 10:25:08 2005
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6, 18 -- From: claire haueter {lukeclaire-at-yahoo.com}
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We use Agarose type IX (Sigma). It works well with Spurrs and Araldite
(from TAAB in the UK). This summer I have had a problem infiltrating
cells in agarose with LR White (polymerising at 55 degrees C) despite
success in two previous years. This is probably a problem with me
rather than the products.

Dave

-----Original Message-----
X-from: lukeclaire-at-yahoo.com [mailto:lukeclaire-at-yahoo.com]
Sent: 10 August 2005 16:26
To: David Patton

Dear Listers,

Has anyone used a low melting point (LMP) agarose to
encapsulate cells for TEM? If yes, which resin is
best suited for LMP agarose encapsulted cells? We
have in the lab Durcupan ACM resin, EMbed-812, LRW
resin kits.

The samples were encapsulated after buffer wash, after
osmium fixation.

Thank you,

Claire

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

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18, 30 -- From David.Patton-at-uwe.ac.uk Wed Aug 10 10:45:23 2005
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I have used LMP many times for TEM. I process it just like I would a
block of tissue and go into EMbed-812 or Spurs.
Good luck
David

On Aug 10, 2005, at 8:29 AM, lukeclaire-at-yahoo.com wrote:

}
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} Dear Listers,
}
} Has anyone used a low melting point (LMP) agarose to
} encapsulate cells for TEM? If yes, which resin is
} best suited for LMP agarose encapsulted cells? We
} have in the lab Durcupan ACM resin, EMbed-812, LRW
} resin kits.
}
} The samples were encapsulated after buffer wash, after
} osmium fixation.
}
} Thank you,
}
} Claire
}
} __________________________________________________
} Do You Yahoo!?
} Tired of spam? Yahoo! Mail has the best spam protection around
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} Headers==============================
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} 6, 18 -- From: claire haueter {lukeclaire-at-yahoo.com}
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==============================Original Headers==============================
5, 22 -- From Elliott-at-arizona.edu Wed Aug 10 10:53:33 2005
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Hello all,

We have a group of labs that are
attempting to standardize an Osmium-
ZnI2 staining procedure for detection of
chloride cells in trout gills.

The protocol seems foolproof: immerse
freshly dissected gill pieces in a freshly-
made solution of 1 part 2% OsO4 to 4
parts 3% ZnI2 for 24 at 4�C, dehydrate,
clear in Histochoice, paraffin-embed,
section, de- paraffinize and mount in
Cytoseal60-xyl.
There seems to be no consistency in
results. In fact, more often or not we see
no staining of the chloride cells, though
the gill filaments themselves display the
rich tan colour of well-osmicated tissue.

Does anyone have experience with this
technique? What can cause it to fail? Is
age/source of reagents an important
factor? Are there any tricks to making
the procedure reliable?

==============================Original Headers==============================
5, 21 -- From AOCHALSK-at-science.uottawa.ca Wed Aug 10 11:14:17 2005
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5, 21 -- Organization: Univ. of/d' Ottawa
5, 21 -- To: Microscopy-at-microscopy.com
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5, 21 -- Subject: LM zinc-osmium staining of trout chloride cells:woes
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On Aug 10, 2005, at 6:30 AM, lfox1-at-lumc.edu wrote:

} Can anyone advise this new user to our lab? His prep/questions are
} below. I think that he needs negative staining. How best to prep this
} sample?/fixation/washing out the sucrose?? and what type of filmed
} grids are being used these days??Formvar/Pioloform??other??
}
} -----------------------
}
} Basically what I have is a 100nm virus particle bound to a 100nm
} liposome molecule by a Nickel NTA "bridge". In short, I would like a
} picture of this complex. So, If I had this complex (usually in
} ~10-20%sucrose solution), what would I do from there as far a
} preparation?
}
Dear Linda,
If the specimen would not be perturbed by removal of the sucrose,
either of the techniques of negative staining or cryoEM would be
improved, and for negative staining with UAc, the buffer should not
contain phosphate. It would be best if the specimen could be in a
dilute buffer, either tris or a Good buffer would be suitable. I would
use carbon-formvar coated grids for negative staining and either lacy
carbon or, better, Quantifoils for cryo imaging. Depending on the
resolution desired, the tolerance of the specimen for low pH, the
equipment available, etc., the easiest procedure (and the one that I
would start with) is negative staining with UAc, which is at pH = ~3,
or phosphotungstate or phosphomolybdate, which are at pH = ~7. After
getting the specimen into the appropriate buffer, put 5 ul of the
appropriately diluted material onto a glow-discharged carbon-formvar
grid. Add 5 ul of a 2% solution of the staining compound, and let
stand for 1 min. Blot most of the liquid off with a bit of #1 filter
paper applied to the edge of the grid, add 10 ul of a 1% solution of
stain, let stand for 1 min, then thoroughly blot off the liquid. After
seeing whether the dilution of the specimen gives a good amount of
material on the grid--enough to see several particles in each (film or
CCD) frame--adjust the concentration if necessary. If cryoEM will be
useful, prepare the specimen at a concentration of about twice that
used for negative staining, and plunge-freeze it.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

==============================Original Headers==============================
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5, 26 -- From: Bill Tivol {tivol-at-caltech.edu}
5, 26 -- Subject: Re: [Microscopy] TEM - liposome NTA bridge prep
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Contents Retrieved from Microscopy Listserver Archives
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Claire,
Note that low melting point agarose (and probably all types)
is nicely stained by fast green. We would make a few percent solution
in 100% ethanol and add a drop to our agarose blobs. Otherwise, they
can vanish in the solutions and lead to some frustrations. I suppose
fast green would also dissolve in acetone but I don't know that for a
fact. However, now we trap small samples between formvar films on
wire loops and like that much better than agarose (except on the days
when the formvar won't cast, 8-().

HTH,
Tobias
}
}
} We use Agarose type IX (Sigma). It works well with Spurrs and Araldite
} (from TAAB in the UK). This summer I have had a problem infiltrating
} cells in agarose with LR White (polymerising at 55 degrees C) despite
} success in two previous years. This is probably a problem with me
} rather than the products.
}
} Dave
}
} -----Original Message-----
} X-from: lukeclaire-at-yahoo.com [mailto:lukeclaire-at-yahoo.com]
} Sent: 10 August 2005 16:26
} To: David Patton
} Subject: [Microscopy] TEM of agar encapsulated sample
}
}
} ----------------
} ----
}
} Dear Listers,
}
} Has anyone used a low melting point (LMP) agarose to
} encapsulate cells for TEM? If yes, which resin is
} best suited for LMP agarose encapsulted cells? We
} have in the lab Durcupan ACM resin, EMbed-812, LRW
} resin kits.
}
} The samples were encapsulated after buffer wash, after
} osmium fixation.
}
} Thank you,
}
} Claire
}
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} 6, 18 -- From: claire haueter {lukeclaire-at-yahoo.com}
} 6, 18 -- Subject: TEM of agar encapsulated sample
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} 18, 30 -- From David.Patton-at-uwe.ac.uk Wed Aug 10 10:45:23 2005
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