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Hi Andy

To save writing out all the options please take a look at our web site HINTS
and TIPS to check where your problem is,
is it the gun, gun chamber, cable or high voltage tank? Do not consider
that the only problem is likely to be the insulation of the gun. Chamber
cleanliness, vacuum level etc all play a part in high voltage breakdown a
feature that will result in beam instability.

But is it beam instability due to the gun (emission meter variations,
virtual source fluttering) or due to contamination in the condenser system
illumination flutter or movement)?

Hope this helps?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel +44 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com

----- Original Message -----
X-from: {aetmicro-at-optonline.net}
To: {protrain-at-emcourses.com}
Sent: Friday, July 29, 2005 9:13 PM

Dear Members
I am trying to accumulate literature sources that explain how best
to prepare insect specimens for sectioning so that the medium adheres well
and such that the knife doesn't 'catch' the specimen and 'drag' it out of
the medium. I would like to start with semi-thin sections of wings, and
then ultra thin for TEM. If there are any 'inside' tricjs that are
unpublished or otherwise unlikely to be encountered, I would be grateful.
Thanks
Ian

Ian Stocks
308 Long Hall
Clemson University
864 656 5058
istocks-at-clemson.edu

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3, 19 -- Subject: LM- embedding insect cuticle
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Post-Doctoral Research Associate

A post-doctoral research associate position is available in the Center
for Solid State Science at Arizona State University. The research
project involves in situ synthesis and characterization of carbon
nanotubes using a state of the art Environmental Transmission Electron
Microscope, Tecnai F20-ETEM/STEM. Applicants must have a Ph.D in
Chemistry, Physics or Materials Science with experience in
high-resolution imaging, electron energy-loss spectroscopy using
transmission electron microscope. Experience in the areas of scanning
transmission electron microscopy, structural and/or theoretical modeling
is desired. The successful candidate will have an advantage of working
with the cutting edge technology and in a highly motivating environment.

The position is for one year with a start date in August. Deadline is
August 15, 2005, if not filled, weekly thereafter until search closed.
Salary is $34,000/year. Interested candidates must send their resume,
list of publications and the name, address, and phone number of three
references to: Dr Renu Sharma, Center for Solid State Science, Arizona
State University, Tempe, AZ 85287-1704. Email renu.sharma-at-asu.edu.

==============================Original Headers==============================
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Dear Ian,
For LM use London Resin and try to match the hardness of the resin to
the tissue eg use the hardest grade for highly sclerotised beetle
cuticle and the medium grade for most cuticles. Tricks like orienting
the block so the knife cuts the cuticle first then cuts through the
underlying tissue will help but the hydrophobic epicuticle of some
insects will never bond to the resin when using standard
glutaraldehyde/osmium fixations. Yell if you want more detail.
Cheers,
Eric Hines
CSIRO Entomology
Canberra OZ

} -----Original Message-----
} From: istocks-at-CLEMSON.EDU [mailto:istocks-at-CLEMSON.EDU]
} Sent: Monday, 1 August 2005 11:26 PM
} To: Hines, Eric (Entomology, Black Mountain)
} Subject: [Microscopy] LM- embedding insect cuticle
}
}
}
}
}
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}
} Dear Members
} I am trying to accumulate literature sources that
} explain how best
} to prepare insect specimens for sectioning so that the medium
} adheres well
} and such that the knife doesn't 'catch' the specimen and
} 'drag' it out of
} the medium. I would like to start with semi-thin sections of
} wings, and
} then ultra thin for TEM. If there are any 'inside' tricjs that are
} unpublished or otherwise unlikely to be encountered, I would
} be grateful. Thanks Ian
}
} Ian Stocks
} 308 Long Hall
} Clemson University
} 864 656 5058
} istocks-at-clemson.edu
}
}
} ==============================Original
} Headers==============================
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} 3, 19 -- Subject: LM- embedding insect cuticle
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3, 29 -- From Eric.Hines-at-csiro.au Mon Aug 1 18:56:56 2005
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.

--- cammer-at-aecom.yu.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
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}
} more "amateur" snowflake pics, 6 web pages beginning
} at
} http://cammer.net/blog/snowflake.htm and ending with
} a movie at
} http://cammer.net/blog/snowflake06.htm
}
} Also a favorite at
}
http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm
}
}
}
}
}
} At 08:49 AM 07/29/05 -0500, you wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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}
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} ----------------------------------------------------------------------------
} }
} } And be sure to check out Wilson A. Bentley's life &
} work. He pioneered
} } snowflake photography a long time ago, and did it
} the hard way. Amateurs
} } rock!
} }
} } Paul Grover
} }
}
} ------------------------------------------------------------------------
} } No matter how much cats fight, there always seem to
} be plenty of kittens.
} } -
} A. Lincoln
} }
} }
} }
} } ==============================Original
} Headers==============================
} } 5, 23 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul
} 29 08:48:35 2005
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} } -0500
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} {pgrover-at-bilbo.bio.purdue.edu}
} } 5, 23 -- To: {microscopy-at-microscopy.com}
} } 5, 23 -- Subject: snowflakes
} } 5, 23 -- Date: Fri, 29 Jul 2005 08:48:35 -0500
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}
}
____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility
} Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park
} Ave. Bronx, NY 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains
} information that is privileged.**
}
}
} ==============================Original
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} 12, 23 -- From cammer-at-aecom.yu.edu Fri Jul 29
} 11:32:11 2005
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} 12, 23 -- Subject: Re: [Microscopy] snowflakes
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}

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

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First the flame: why assume we only like looking at little itty bitty
things?

On to real comments: Actually, I like looking at anything (size doesn't
count) and I like making cool and/or pretty pictures and I like math puzzles.

Being a microscopist fulfills all of these and pays my bills. To support
my job and my interests, to have access to the cool materials and
technologies that keep me fascinated, I need to be concerned with the
latest multi-probe cutting edge technologies, running a multi-user
facility, having a
purchasing department, federal grants, personnel and a job. Such is reality.

The conclusion is:
We should be discussing both the microscopies (cool pictures and how to
make them regardless whether they are snowflakes for the elementary
classroom or biological processes to understand cutting edge protein
interactions) and the processes for supporting the microscopies (e.g.
federal grants vs. commercial).

I've been subscribed here for years. It seems to me this listserv is doing
exactly what I describe/prescribe. Do we really need to discuss this more?

-Michael

} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll waste a little
} bandwidth and a few KB to vent. I'd like to note that:
}
} (1) This is a MICROSCOPY listserver. This apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one must be using the latest
} multi-probe cutting edge technology, run a multi-user facility, or have a
} purchasing department, federal grant, or even a job. Our common bond is
} that we like to "look at little things".

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**

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9, 23 -- To: microscopy-at-microscopy.com
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9, 23 -- Subject: Re: [Microscopy] raison d' etre
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Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey
with vapors of osmium over the years. Although we are
taking every precautions to avoid leakage of osmium
vapor from its container, this is a problem that I have
observed in every EM lab that I have worked in. Does
someone on the list have a method to store osmium in such
a way as to avoid any escape of vapor? Also, is there
something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

==============================Original Headers==============================
5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48])
5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j72G9BnV011512
5, 18 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2005 11:09:11 -0500
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5, 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005 12:09:29 -0400 (EDT)
5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
5, 18 -- Subject: Osmium vapors
5, 18 -- In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
5, 18 -- To: microscopy-at-microscopy.com
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he was just kidding around you know.

--- cammer-at-aecom.yu.edu wrote:

}
}
}
}
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}
} First the flame: why assume we only like looking at
} little itty bitty
} things?
}
} On to real comments: Actually, I like looking at
} anything (size doesn't
} count) and I like making cool and/or pretty pictures
} and I like math puzzles.
}
} Being a microscopist fulfills all of these and pays
} my bills. To support
} my job and my interests, to have access to the cool
} materials and
} technologies that keep me fascinated, I need to be
} concerned with the
} latest multi-probe cutting edge technologies,
} running a multi-user
} facility, having a
} purchasing department, federal grants, personnel and
} a job. Such is reality.
}
} The conclusion is:
} We should be discussing both the microscopies (cool
} pictures and how to
} make them regardless whether they are snowflakes for
} the elementary
} classroom or biological processes to understand
} cutting edge protein
} interactions) and the processes for supporting the
} microscopies (e.g.
} federal grants vs. commercial).
}
} I've been subscribed here for years. It seems to me
} this listserv is doing
} exactly what I describe/prescribe. Do we really
} need to discuss this more?
}
} -Michael
}
} } Esteemed Microscopists,
} }
} } Since most of you apparently are in Hawaii now,
} I'll waste a little
} } bandwidth and a few KB to vent. I'd like to note
} that:
} }
} } (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} } little things".
} }
} } (2) There is apparently no requirement that one
} must be using the latest
} } multi-probe cutting edge technology, run a
} multi-user facility, or have a
} } purchasing department, federal grant, or even a
} job. Our common bond is
} } that we like to "look at little things".
}
}
____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility
} Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park
} Ave. Bronx, NY 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains
} information that is privileged.**
}
}
} ==============================Original
} Headers==============================
} 9, 23 -- From cammer-at-aecom.yu.edu Tue Aug 2
} 11:05:59 2005
} 9, 23 -- Received: from mailgw.aecom.yu.edu
} (mailgw.aecom.yu.edu [129.98.1.16])
} 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j72G5wXq005120
} 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2
} Aug 2005 11:05:59 -0500
} 9, 23 -- Received: from mailvx.aecom.yu.edu
} (mailvx.aecom.yu.edu [129.98.1.17])
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} with SMTP id j72G5Jb5023564
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} Aug 2005 12:05:58 -0400
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} ([129.98.1.100])
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} with SMTP id M2005080212055813185
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} Aug 2005 12:05:58 -0400
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} (aif3.aif.aecom.yu.edu [129.98.30.137])
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} Aug 2005 12:05:58 -0400 (EDT)
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}
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} 9, 23 -- X-Sender: cammer-at-mailserver.aecom.yu.edu
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} 9, 23 -- Date: Tue, 02 Aug 2005 12:05:58 -0400
} 9, 23 -- To: microscopy-at-microscopy.com
} 9, 23 -- From: Michael Cammer {cammer-at-aecom.yu.edu}
} 9, 23 -- Subject: Re: [Microscopy] raison d' etre
} 9, 23 -- In-Reply-To:
} {200507291437.j6TEboiR011778-at-ns.microscopy.com}
} 9, 23 -- Mime-Version: 1.0
} 9, 23 -- Content-Type: text/plain;
} charset="us-ascii"; format=flowed
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}

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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:11:28 2005
5, 19 -- Received: from web50204.mail.yahoo.com (web50204.mail.yahoo.com [206.190.38.45])
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5, 19 -- Date: Tue, 2 Aug 2005 09:11:26 -0700 (PDT)
5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] Re: raison d' etre
5, 19 -- To: microscopy-at-microscopy.com
5, 19 -- In-Reply-To: {200508021607.j72G7KJW007670-at-ns.microscopy.com}
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you could try placing the bottle in a bath of oil, but
that would get messy.

--- marc.pypaert-at-yale.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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}
} Question to the list:
}
} Our local safety inspector is concerned about the
} appearance
} of the fridge where we stock our solution of osmium.
} As you
} can expect the white interior of the fridge has
} turned grey
} with vapors of osmium over the years. Although we
} are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I
} have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in
} such
} a way as to avoid any escape of vapor? Also, is
} there
} something we could put into fridge that would trap
} osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original
} Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} 11:09:11 2005
} 5, 18 -- Received: from BIOMED.MED.YALE.EDU
} (biomed.med.yale.edu [130.132.232.48])
} 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j72G9BnV011512
} 5, 18 -- for {microscopy-at-microscopy.com} ; Tue, 2
} Aug 2005 11:09:11 -0500
} 5, 18 -- Received: from yale.edu
} (net234-111.med.yale.edu [130.132.234.111])
} 5, 18 -- by biomed.med.yale.edu (PMDF V6.1-1
} #30532)
} 5, 18 -- with ESMTP id
} {01LRCOV1R88000DGI6-at-biomed.med.yale.edu} for
} 5, 18 -- microscopy-at-microscopy.com; Tue, 02 Aug
} 2005 12:09:29 -0400 (EDT)
} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
} 5, 18 -- In-reply-to:
} {200508021530.j72FU83w031285-at-ns.microscopy.com}
} 5, 18 -- To: microscopy-at-microscopy.com
} 5, 18 -- Message-id:
} {C13CE8F5-036F-11DA-AC3D-0030659833B4-at-yale.edu}
} 5, 18 -- MIME-version: 1.0 (Apple Message framework
} v553)
} 5, 18 -- X-Mailer: Apple Mail (2.553)
} 5, 18 -- Content-type: text/plain; format=flowed;
} charset=US-ASCII
} 5, 18 -- Content-transfer-encoding: 7bit
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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:13:12 2005
5, 19 -- Received: from web50209.mail.yahoo.com (web50209.mail.yahoo.com [206.190.38.50])
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5, 19 -- Date: Tue, 2 Aug 2005 09:13:11 -0700 (PDT)
5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] Osmium vapors
5, 19 -- To: microscopy-at-microscopy.com
5, 19 -- In-Reply-To: {200508021610.j72GAeX8015207-at-ns.microscopy.com}
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i was trying to send that directly back to poster. my
bad i guess.

--- hoffpajo-at-yahoo.com wrote:

}
}
}
}
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}
} he was just kidding around you know.
}
} --- cammer-at-aecom.yu.edu wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
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} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------------
} }
} } First the flame: why assume we only like looking
} at
} } little itty bitty
} } things?
} }
} } On to real comments: Actually, I like looking at
} } anything (size doesn't
} } count) and I like making cool and/or pretty
} pictures
} } and I like math puzzles.
} }
} } Being a microscopist fulfills all of these and
} pays
} } my bills. To support
} } my job and my interests, to have access to the
} cool
} } materials and
} } technologies that keep me fascinated, I need to be
} } concerned with the
} } latest multi-probe cutting edge technologies,
} } running a multi-user
} } facility, having a
} } purchasing department, federal grants, personnel
} and
} } a job. Such is reality.
} }
} } The conclusion is:
} } We should be discussing both the microscopies
} (cool
} } pictures and how to
} } make them regardless whether they are snowflakes
} for
} } the elementary
} } classroom or biological processes to understand
} } cutting edge protein
} } interactions) and the processes for supporting the
} } microscopies (e.g.
} } federal grants vs. commercial).
} }
} } I've been subscribed here for years. It seems to
} me
} } this listserv is doing
} } exactly what I describe/prescribe. Do we really
} } need to discuss this more?
} }
} } -Michael
} }
} } } Esteemed Microscopists,
} } }
} } } Since most of you apparently are in Hawaii now,
} } I'll waste a little
} } } bandwidth and a few KB to vent. I'd like to note
} } that:
} } }
} } } (1) This is a MICROSCOPY listserver. This
} } apparently means "looking at
} } } little things".
} } }
} } } (2) There is apparently no requirement that one
} } must be using the latest
} } } multi-probe cutting edge technology, run a
} } multi-user facility, or have a
} } } purchasing department, federal grant, or even a
} } job. Our common bond is
} } } that we like to "look at little things".
} }
} }
}
____________________________________________________________________________
} } Michael Cammer Analytical Imaging Facility
} } Albert Einstein Coll. of Med.
} } Jack & Pearl Resnick Campus 1300 Morris Park
} } Ave. Bronx, NY 10461
} } (718) 430-2890 Fax: 430-8996 URL:
} } http://www.aecom.yu.edu/aif/
} } **This electronic transmission contains
} } information that is privileged.**
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 23 -- From cammer-at-aecom.yu.edu Tue Aug 2
} } 11:05:59 2005
} } 9, 23 -- Received: from mailgw.aecom.yu.edu
} } (mailgw.aecom.yu.edu [129.98.1.16])
} } 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id j72G5wXq005120
} } 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2
} } Aug 2005 11:05:59 -0500
} } 9, 23 -- Received: from mailvx.aecom.yu.edu
} } (mailvx.aecom.yu.edu [129.98.1.17])
} } 9, 23 -- by mailgw.aecom.yu.edu (8.12.11/8.12.11)
} } with SMTP id j72G5Jb5023564
} } 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2
} } Aug 2005 12:05:58 -0400
} } 9, 23 -- Received: from post.aecom.yu.edu
} } ([129.98.1.100])
} } 9, 23 -- by mailvx.aecom.yu.edu (SAVSMTP
} 3.1.1.32)
} } with SMTP id M2005080212055813185
} } 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 02
} } Aug 2005 12:05:58 -0400
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} } (aif3.aif.aecom.yu.edu [129.98.30.137])
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} ESMTP
} } id 91E0B2FC6
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} } Aug 2005 12:05:58 -0400 (EDT)
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} }
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} } 9, 23 -- X-Sender: cammer-at-mailserver.aecom.yu.edu
} } 9, 23 -- X-Mailer: QUALCOMM Windows Eudora Version
} } 5.2.1
} } 9, 23 -- Date: Tue, 02 Aug 2005 12:05:58 -0400
} } 9, 23 -- To: microscopy-at-microscopy.com
} } 9, 23 -- From: Michael Cammer
} {cammer-at-aecom.yu.edu}
} } 9, 23 -- Subject: Re: [Microscopy] raison d' etre
} } 9, 23 -- In-Reply-To:
} } {200507291437.j6TEboiR011778-at-ns.microscopy.com}
} } 9, 23 -- Mime-Version: 1.0
} } 9, 23 -- Content-Type: text/plain;
} } charset="us-ascii"; format=flowed
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} } Headers==============================
} }
}
}
} __________________________________________________
} Do You Yahoo!?
} Tired of spam? Yahoo! Mail has the best spam
} protection around
} http://mail.yahoo.com
}
} ==============================Original
} Headers==============================
} 5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:11:28
} 2005
} 5, 19 -- Received: from web50204.mail.yahoo.com
} (web50204.mail.yahoo.com [206.190.38.45])
} 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Tuesday, August 02, 2005 11:13 AM
To: msimms-at-tracelabs.com

Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey
with vapors of osmium over the years. Although we are
taking every precautions to avoid leakage of osmium
vapor from its container, this is a problem that I have
observed in every EM lab that I have worked in. Does
someone on the list have a method to store osmium in such
a way as to avoid any escape of vapor? Also, is there
something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

==============================Original Headers==============================
5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu
[130.132.232.48])
5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
j72G9BnV011512
5, 18 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2005 11:09:11
-0500
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5, 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005 12:09:29 -0400 (EDT)
5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
5, 18 -- Subject: Osmium vapors
5, 18 -- In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
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==============================Original Headers==============================
16, 15 -- From msimms-at-tracelabs.com Tue Aug 2 11:19:49 2005
16, 15 -- Received: from mail0.methode.com (mail0.methode.com [12.182.227.149])
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Marc

We store our osmium in a secondary container...a large poly pro bottle
(capped) and then we bag that container in a plastic Ziploc. It helps a
bit.

Dorrance

-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Tuesday, August 02, 2005 9:10 AM
To: McLean, Dorrance

Question to the list:

Our local safety inspector is concerned about the appearance of the
fridge where we stock our solution of osmium. As you can expect the
white interior of the fridge has turned grey with vapors of osmium over
the years. Although we are taking every precautions to avoid leakage of
osmium vapor from its container, this is a problem that I have observed
in every EM lab that I have worked in. Does someone on the list have a
method to store osmium in such a way as to avoid any escape of vapor?
Also, is there something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

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16, 29 -- From dmclea-at-sandia.gov Tue Aug 2 11:23:07 2005
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When OsO4 came in metal cans, I found by placing the working solution in a
Wheaton dropper bottle, wrap the bulb with Parafilm, place the bottle in the
can, close the lid, wrap the lid with electrician tape, and place the can in
a plastic Ziplock bag worked great. You could essentially use any clean
glass container, not just a Wheaton bottle.

After 20 years of working with OsO4, the inside of the refrigerator was
pristine. It requires diligence to unwrap and wrap the container, but it
worked.

A short-cut to the above mentioned technique, was to use a clean pint paint,
place the Wheaton bottle in the can and push the lid down tight, then place
the entire can in a Ziplock bag.

Regards,
Ken
_______________________________________
Kenneth L. Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N Willamette Blvd.
Portland, OR 97203 USA

Tel.: 503.943.8861
Email: tiekotte-at-up.edu

On 8/2/05 9:09 AM, "marc.pypaert-at-yale.edu" {marc.pypaert-at-yale.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
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} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
} 5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
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9, 17 -- Subject: Re: [Microscopy] Osmium vapors
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in the alternative and it may be more expensive and
you may not want to go there. and if someone with a
better memory than i do anymore, can correct me. i do
remember seeing premade OsO4 in small amounts. use
what you need, perhaps even batch the tissue samples.
then throw out the unused in the waste.
just a thought.

--- dmclea-at-sandia.gov wrote:

}
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}
} Marc
}
} We store our osmium in a secondary container...a
} large poly pro bottle
} (capped) and then we bag that container in a plastic
} Ziploc. It helps a
} bit.
}
} Dorrance
}
} -----Original Message-----
} X-from: marc.pypaert-at-yale.edu
} [mailto:marc.pypaert-at-yale.edu]
} Sent: Tuesday, August 02, 2005 9:10 AM
} To: McLean, Dorrance
} Subject: [Microscopy] Osmium vapors
}
}
}
}
}
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} America To Subscribe/Unsubscribe --
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} ----
}
} Question to the list:
}
} Our local safety inspector is concerned about the
} appearance of the
} fridge where we stock our solution of osmium. As you
} can expect the
} white interior of the fridge has turned grey with
} vapors of osmium over
} the years. Although we are taking every precautions
} to avoid leakage of
} osmium vapor from its container, this is a problem
} that I have observed
} in every EM lab that I have worked in. Does someone
} on the list have a
} method to store osmium in such a way as to avoid any
} escape of vapor?
} Also, is there something we could put into fridge
} that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original
} Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} 11:09:11 2005 5, 18 --
} Received: from BIOMED.MED.YALE.EDU
} (biomed.med.yale.edu
} [130.132.232.48])
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} ESMTP id
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5, 18
} -- From: Marc
} Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} Osmium vapors 5, 18 --
} In-reply-to:
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} 2005
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____________________________________________________
Start your day with Yahoo! - make it your home page
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We use Osmium in our lab and keep it in the refrigerator.
We keep it in a glass bottle and cover the lid with
parafilm. Then we put that bottle in another bottle with a
screw top and wrap the bottle in foil. It works great, our
refrigerator doesn't have and black residue, just the two
bottles.

rebecca

==============================Original Headers==============================
2, 15 -- From gunkelrl-at-slu.edu Tue Aug 2 11:47:48 2005
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is mannie Mannie Steglich still around? haven't heard
from him in a while.
john

--- hoffpajo-at-yahoo.com wrote:

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}
} in the alternative and it may be more expensive and
} you may not want to go there. and if someone with a
} better memory than i do anymore, can correct me. i
} do
} remember seeing premade OsO4 in small amounts. use
} what you need, perhaps even batch the tissue
} samples.
} then throw out the unused in the waste.
} just a thought.
}
} --- dmclea-at-sandia.gov wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
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} }
}
----------------------------------------------------------------------------
} }
} } Marc
} }
} } We store our osmium in a secondary container...a
} } large poly pro bottle
} } (capped) and then we bag that container in a
} plastic
} } Ziploc. It helps a
} } bit.
} }
} } Dorrance
} }
} } -----Original Message-----
} } X-from: marc.pypaert-at-yale.edu
} } [mailto:marc.pypaert-at-yale.edu]
} } Sent: Tuesday, August 02, 2005 9:10 AM
} } To: McLean, Dorrance
} } Subject: [Microscopy] Osmium vapors
} }
} }
} }
} }
} }
}
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} } Question to the list:
} }
} } Our local safety inspector is concerned about the
} } appearance of the
} } fridge where we stock our solution of osmium. As
} you
} } can expect the
} } white interior of the fridge has turned grey with
} } vapors of osmium over
} } the years. Although we are taking every
} precautions
} } to avoid leakage of
} } osmium vapor from its container, this is a problem
} } that I have observed
} } in every EM lab that I have worked in. Does
} someone
} } on the list have a
} } method to store osmium in such a way as to avoid
} any
} } escape of vapor?
} } Also, is there something we could put into fridge
} } that would trap osmium
} } vapors as they leak out? Thanks for your advise.
} }
} } Marc
} }
} } --
} } Marc Pypaert
} } Department of Cell Biology
} } Center for Cell and Molecular Imaging
} } Ludwig Institute for Cancer Research
} } Yale University School of Medicine
} } 333 Cedar Street, PO Box 208002
} } New Haven, CT 06520-8002
} } TEL 203-785 3681
} } FAX 203-785 7446
} }
} }
} } ==============================Original
} } Headers==============================
} } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } 11:09:11 2005 5, 18 --
} } Received: from BIOMED.MED.YALE.EDU
} } (biomed.med.yale.edu
} } [130.132.232.48])
} } 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id
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} } Aug 2005
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} } 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5,
} 18
} } -- From: Marc
} } Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} } Osmium vapors 5, 18 --
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} }
} }
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} } 16, 29 -- From dmclea-at-sandia.gov Tue Aug 2
} 11:23:07
} } 2005
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} } (mm01snlnto.sandia.gov [132.175.109.20])
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5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:49:26 2005
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Working with undergraduates in a small (yet multiuser) lab, I have had
concerns about handling and storing osmium tetroxide for years. My
solution (which may not be practical for everyone) is that only sealed
ampoules are stored in the fridge (in their original shipping can and
packing materials). Vials are opened one at a time, the solutions are
mixed and used, tissues are exposed, left-over supplies are stored, and
waste is kept ONLY in the fume hood. In other words, opened supplies of
osmium never leave the fume hood (until disposal as toxic waste). If a
cold solution is needed, and ice bath can be used. We mix just about
enough for each procedure, and the unused left-over Os04 working
solution is kept in a small glass jar in the hood for the next
procedure. So we have no blackened refrigerator surfaces and no Os04
fumes accumulating in the fridge (or escaping into the room). It seems
to me that this approach could be expanded for a larger lab.

--Jan

---------------------------------------8/2/05
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College
State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------

marc.pypaert-at-yale.edu wrote:8/2/05
} ----------------------------------------------------------------------------
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}
} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
} 5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48])
} 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j72G9BnV011512
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
} 5, 18 -- In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
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==============================Original Headers==============================
5, 22 -- From jfactor-at-ns.purchase.edu Tue Aug 2 11:51:39 2005
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5, 22 -- From: Jan Factor {jfactor-at-ns.purchase.edu}
5, 22 -- Subject: Re: [Microscopy] Osmium vapors
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Still alive and kikking in Houston.

hoffpajo-at-yahoo.com

08/02/2005 11:52 AM
Please respond to microscopy

To:
msteglic-at-mdanderson.org
cc:

is mannie Mannie Steglich still around? haven't heard
from him in a while.
john

--- hoffpajo-at-yahoo.com wrote:

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}
} in the alternative and it may be more expensive and
} you may not want to go there. and if someone with a
} better memory than i do anymore, can correct me. i
} do
} remember seeing premade OsO4 in small amounts. use
} what you need, perhaps even batch the tissue
} samples.
} then throw out the unused in the waste.
} just a thought.
}
} --- dmclea-at-sandia.gov wrote:
}
} }
} }
} }
} }
}
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} }
} } Marc
} }
} } We store our osmium in a secondary container...a
} } large poly pro bottle
} } (capped) and then we bag that container in a
} plastic
} } Ziploc. It helps a
} } bit.
} }
} } Dorrance
} }
} } -----Original Message-----
} } X-from: marc.pypaert-at-yale.edu
} } [mailto:marc.pypaert-at-yale.edu]
} } Sent: Tuesday, August 02, 2005 9:10 AM
} } To: McLean, Dorrance
} } Subject: [Microscopy] Osmium vapors
} }
} }
} }
} }
} }
}
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} } Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
------------------------------------------------------------------------
} } ----
} }
} } Question to the list:
} }
} } Our local safety inspector is concerned about the
} } appearance of the
} } fridge where we stock our solution of osmium. As
} you
} } can expect the
} } white interior of the fridge has turned grey with
} } vapors of osmium over
} } the years. Although we are taking every
} precautions
} } to avoid leakage of
} } osmium vapor from its container, this is a problem
} } that I have observed
} } in every EM lab that I have worked in. Does
} someone
} } on the list have a
} } method to store osmium in such a way as to avoid
} any
} } escape of vapor?
} } Also, is there something we could put into fridge
} } that would trap osmium
} } vapors as they leak out? Thanks for your advise.
} }
} } Marc
} }
} } --
} } Marc Pypaert
} } Department of Cell Biology
} } Center for Cell and Molecular Imaging
} } Ludwig Institute for Cancer Research
} } Yale University School of Medicine
} } 333 Cedar Street, PO Box 208002
} } New Haven, CT 06520-8002
} } TEL 203-785 3681
} } FAX 203-785 7446
} }
} }
} } ==============================Original
} } Headers==============================
} } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } 11:09:11 2005 5, 18 --
} } Received: from BIOMED.MED.YALE.EDU
} } (biomed.med.yale.edu
} } [130.132.232.48])
} } 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id
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} } Aug 2005
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} } 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005
} } 12:09:29 -0400 (EDT)
} } 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5,
} 18
} } -- From: Marc
} } Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} } Osmium vapors 5, 18 --
} } In-reply-to:
} } {200508021530.j72FU83w031285-at-ns.microscopy.com}
} } 5, 18 -- To: microscopy-at-microscopy.com
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} }
} }
} } ==============================Original
} } Headers==============================
} } 16, 29 -- From dmclea-at-sandia.gov Tue Aug 2
} 11:23:07
} } 2005
} } 16, 29 -- Received: from MM01SNLNTO.sandia.gov
} } (mm01snlnto.sandia.gov [132.175.109.20])
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The simplest solution to this problem is not to keep your osmium in the
refrigerator. I keep mine in a glass Schott bottle with plastic cap which
is then stored within a plastic container in the fume hood. I am not sure
if light effects the osmium but to be sure, I wrap the plastic container in
aluminum foil. There is leakage from the glass bottle because over time,
the inside of the plastic container goes black. Anything that leaks past
the secondary container goes up the fume hood. Once every year or two, I
splurge and replace the bottle and container. I don't experience
"pre-mature" darkening of my 2% osmium stock which typically lasts about
2-3 months before I use it all up. Tom Phillips

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

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well thats good to know. is it cooler there than here?

--- msteglic-at-mdanderson.org wrote:

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} Still alive and kikking in Houston.
}
}
}
}
}
} hoffpajo-at-yahoo.com
}
} 08/02/2005 11:52 AM
} Please respond to microscopy
}
}
}
}
} To:
} msteglic-at-mdanderson.org
} cc:
}
}
}
}
}
} Subject:
} [Microscopy] Re: Mannie Steglich
}
}
}
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}
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}
} is mannie Mannie Steglich still around? haven't
} heard
} from him in a while.
} john
}
} --- hoffpajo-at-yahoo.com wrote:
}
} }
} }
} }
} }
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} }
} } in the alternative and it may be more expensive
} and
} } you may not want to go there. and if someone with
} a
} } better memory than i do anymore, can correct me. i
} } do
} } remember seeing premade OsO4 in small amounts. use
} } what you need, perhaps even batch the tissue
} } samples.
} } then throw out the unused in the waste.
} } just a thought.
} }
} } --- dmclea-at-sandia.gov wrote:
} }
} } }
} } }
} } }
} } }
} }
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} } }
} } } Marc
} } }
} } } We store our osmium in a secondary container...a
} } } large poly pro bottle
} } } (capped) and then we bag that container in a
} } plastic
} } } Ziploc. It helps a
} } } bit.
} } }
} } } Dorrance
} } }
} } } -----Original Message-----
} } } X-from: marc.pypaert-at-yale.edu
} } } [mailto:marc.pypaert-at-yale.edu]
} } } Sent: Tuesday, August 02, 2005 9:10 AM
} } } To: McLean, Dorrance
} } } Subject: [Microscopy] Osmium vapors
} } }
} } }
} } }
} } }
} } }
} }
}
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} } } The Microscopy ListServer -- CoSponsor: The
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} } } ----
} } }
} } } Question to the list:
} } }
} } } Our local safety inspector is concerned about
} the
} } } appearance of the
} } } fridge where we stock our solution of osmium. As
} } you
} } } can expect the
} } } white interior of the fridge has turned grey
} with
} } } vapors of osmium over
} } } the years. Although we are taking every
} } precautions
} } } to avoid leakage of
} } } osmium vapor from its container, this is a
} problem
} } } that I have observed
} } } in every EM lab that I have worked in. Does
} } someone
} } } on the list have a
} } } method to store osmium in such a way as to avoid
} } any
} } } escape of vapor?
} } } Also, is there something we could put into
} fridge
} } } that would trap osmium
} } } vapors as they leak out? Thanks for your advise.
} } }
} } } Marc
} } }
} } } --
} } } Marc Pypaert
} } } Department of Cell Biology
} } } Center for Cell and Molecular Imaging
} } } Ludwig Institute for Cancer Research
} } } Yale University School of Medicine
} } } 333 Cedar Street, PO Box 208002
} } } New Haven, CT 06520-8002
} } } TEL 203-785 3681
} } } FAX 203-785 7446
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } } 11:09:11 2005 5, 18 --
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} } } (biomed.med.yale.edu
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} } } Aug 2005
}
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The last I knew he was at MD Anderson in Texas - but he may well have
retired by now - see http://woodenwonderstx.com/About.html

At 12:49 PM 8/2/2005, you wrote:

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tttan-at-simtech.a-star.edu.sg) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
August 1, 2005 at 20:24:16
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Email: tttan-at-simtech.a-star.edu.sg
Name: TT Tan

Organization: Singapore Inst. of Manuf. Tech.

Title-Subject: [Filtered] Field Ion Microscope

Question: Hi all,

I would like to know from who can I buy the glassware to do field ion
microscopy.

I am looking for a quartz ware that can do the job. Preferably one
that allows me to fit onto a NW40 joint.

Thanks in advance.

---------------------------------------------------------------------------

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (winston.wiggins-at-cshs.org) from
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
Tuesday, August 2, 2005 at 12:15:04
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Email: winston.wiggins-at-cshs.org
Name: Winston Wiggins

Organization: Cedars-Sinai HealthCare System

Title-Subject: [Filtered] MListserver: Osmium Vapors

Question: I keep my working solution of Osmium in a glass Wheaton or
Gibco bottle with a Teflon-lined cap and wrap the cap with Parafilm
and put that into the metal can that's shipped with the ampoules of
Osmium or a larger glass jar. I layer molecular sieves in the
can/jar. Any escaping Osmium vapors are indicated by blackening
Parafilm... or blackening refrigerator!
Our frig is clean.

Plastic is permeable to Osmium - use glass!

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sswaffe-at-abv.bg) from
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on Tuesday, August 2, 2005 at 12:24:49
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Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

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Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

330-668-2235 Ext. 238

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NA is 0.5 of AA. Or more correctly NA = n(sine of (AA/2)). The notation n
is the refractive index between your lens and the glass slide or cover
slip, usually air n=1.0000. This isn't very helpful is it? AA or angular
aperture is a measure of the angle which describes the largest come of
light your objective will accept or your condenser will produce.
Resolution depends (simple theory) capturing as many of the defracted rays
from a sample as possible. The bigger the cone the more rays you capture
and the more resolution you have and the more you can magnify the sample.

Optics meant to be used in air always have an NA less than 1. Those using
oil, glycine or water have a NA greater than one. Condensers typically
have a higher NA than your assortment of objectives because they have to
accommodate many objectives. You reduce the NA of a condenser by closing
the condenser iris down. If you have the correct illumination set up you
can remove an eyepiece and watch the condenser iris close in the back focal
plane of the objective. Most of us close it a little past the edge of the
objective back focal plane 'cause we like it contrasty.

So your condenser is an meant to use in air while your objective is an oil
emersion. Never, never, say it with me, never put beans in your nose or
oil on a condenser with an NA of 0.9.

The best resolution you can get with your system is to oil the objective to
the slide and use the condenser in air. Frankly, it's just OK that way and
in my opinion it's oil immersion is never worth the work (OK you can flame
me now...) You need to oil both the condenser and objective to the slide
(assuming you have the right thickness slide, cover slip and your sample
has sufficient contrast by staining or difference in refractive index) to
get the most out of your optical system. I'm a microscopist cause I'm a
big sloppy guy and this is all too much trouble for me.

Sound like a homework question, so good luck!!!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

330-668-2235 Ext. 238

This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
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received this transmission in error, please immediately notify the sender
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attachments without reading or saving in any manner. Thank you.

sswaffe-at-abv.bg
To: frank.karl-at-degussa.com
08/02/2005 02:28 cc:
PM Subject: [Microscopy] AskAMicroscopist: numerical aperture
Please respond to
microscopy

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Below is the result of your feedback form (NJZFM-ultra-55). It was
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on Tuesday, August 2, 2005 at 12:24:49
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

---------------------------------------------------------------------------

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Dear Sophia,

In air a na of .9 is all that is possible with out oiling the
condenser to the slide. Condensers made with .9 na make better
images with out oil than 1.2 na to 1.4 na oil immersion
condensers with out oil. Also simple Abby condensers have a good
deal of chromic aberration at wide apertures.

The combination of those and the fact that most users don't take
the time to oil the condenser to the bottom of the slide
resulted in many microscope makers using .9 na condensers. For a
in depth discussion of the problems of large na condenser and
other things see Ted Clark's piece
http://www.modernmicroscopy.com/main.asp?article=53 in Modern
Microscopy. Other work on lighting by Ted Clarke is referenced
at http://www.couger.com/microscope/Ted-Clarke/

You asked a good questioning about the aperture of the
condenser. To get the full performance form an objective the
condenser and light train need to be the same quality and na as
the objective. That is usually only a real consideration for
study at high resolution and high resolution microphotography.
The limiting factor in any optic system is its weakest link. In
micosopes this often the lighting and/or condeser.

Best Regards
Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org
sswaffe-at-abv.bg wrote:

} Email: sswaffe-at-abv.bg
} Name: Veselin Andreev
}
} Organization: National High School of Mathematics and Science
}
} Education: 9-12th Grade High School
}
} Location: Sofia, Bulgaria
}
} Question: What exactly is the N.A. or numerical aperture and
why on
} my condensor it's value is 0.9 and on my high power objetive
it is
} 1.25?

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The numerical aperture is a rating for how much light comes through. A
variety of design factors that I can't explain affect the value. An
excellent source of information for your class is available on the Olympus
site. I've pasted the address of the page discussing objectives and N.A.
below. From there you can navigate throughout their entire primer.

http://olympusmicro.com/primer/anatomy/objectives.html

I don't have any connection to Olympus and other manufacturers may also have
excellent technical information up on the web. I suggested this site
because I found it to be very well organized, illustrated, and easy to
understand. Aside from the liberal use of the "Olympus" label, the
discussions are based on science, not a sales pitch. I hope this helps.

Walt Klonowski
Pikes Peak Test Labs

-----Original Message-----
X-from: sswaffe-at-abv.bg [mailto:sswaffe-at-abv.bg]
Sent: Tuesday, August 02, 2005 12:31 PM
To: WaltK-at-pptli.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sswaffe-at-abv.bg) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 2, 2005 at 12:24:49
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

---------------------------------------------------------------------------

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Dear Veselin,

The numerical aperture is the light collecting ability of a specific piece of optics. It depends on two factors: half the actual collecting angle and the refractive index of the immersion fluid. The equation is:
NA=n x sin a where n = ri of the immersion fluid (air is 1.00, oil is 1.5212, water is 1.33, for example) and " a" is the half angle.

Your question is an interesting one for several reasons.

First, both the NA of the condenser and the NA of the objective contribute to resolution: (Equations are difficult to send by email, but here is the basic equation:)
R = [1.2 L]/[NAo + NAc] where 1.2 is a shape factor (Bessel function) reflecting the round apertures we use in the microscope, "L" is the wavelength of light (you can use 500nm or 0.500microns as an average), and NAo = NA objective; NAc = NA condenser.

Interestingly, this equation is related to not only the ability to resolve spacing (R is actually the smallest distance between two objects which still permits them to be imaged as 2 separate entities), it also has impact on edge fidelity. As a result, higher NA optics (both objective and condenser) not only improve your ability to resolve fine detail, they also produce crisper, sharper edges.

Secondly, the NA's written on your glassware are only the general operating specs. If you close down the iris in the condenser, you limit the NA of the condenser. Since your condenser has a maximum NA of 0.9,it is not meant to be used with oil. On the other hand, your objective NA indicates that, for best imaging, a drop of the appropriate immersion oil should be placed between it and the top of your sample. If you don't follow this procedure, your images will not only lack clarity (immersion oil is considered to be an important optical component of the system), your objective will only operate with a "working NA" of approximately 0.9.

Finally, your question is very appropriate because, for optimum resolution, the NA of the condenser should match or exceed the NA of your objective. Your condenser does not meet this requirement. If you do the calculations with the Resolution equation above, you will see that you will limit the resolution available from your system. Since you are working at the High School level, this limitation will probably not have a serious impact on your work. If you eventually get involved with higher level research, I would recommend that you purchase a condenser which better fits your needs.

All of this is explained in "Optimizing Light Microscopy," a book still available through MME. For further information, please contact Ken Piel at kenpiel-at-mme1.com. (Tell him I sent you).

Hope this is helpful and good luck in your studies!

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.

At 03:30 PM 8/2/2005, waltk-at-pptli.com wrote:

} ----------------------------------------------------------------------------
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Hello to you all,

We keep our osmium solutions in Schott Duran laboratory bottles
(http://www.schott-duran.com/english/products/duran/detail/laborflaschen
.html), and keep the bottles in a paint can (a new one that never had
paint in it). This is then kept in our fridge. This arrangement seems to
keep the vapour contained (no marks in our fridge) and isn't a pain to
open up. I also keep a pottle of whole milk powder in there for spills.

Aaron Hicks
Electron Microscopy Preparation Technician

Comparative Physiology and Anatomy
Institute of Veterinary, Animal, and Biomedical Sciences
Massey University

PN-412
Private Bag 11 222
Palmerston North
New Zealand

Phone +64 06 350 4874

-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Wednesday, 3 August 2005 4:12 a.m.
To: Hicks, Aaron

Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey with vapors
of osmium over the years. Although we are taking every precautions to
avoid leakage of osmium vapor from its container, this is a problem that
I have observed in every EM lab that I have worked in. Does someone on
the list have a method to store osmium in such a way as to avoid any
escape of vapor? Also, is there something we could put into fridge that
would trap osmium vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446

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Osmium vapours are not stopped by simple bottle tops. We buy ours
already made up. The bottle lids have a plastic (?) insert which is
effective.

Waste osmium is stored in any bottle which is then kept in what we can
in the UK a Kilner jar. It is made of glass, has a rubber seal and uses
wire to clamp the lid very tightly. Osmium vapours do not seem to pass
through rubber seals. Unfortunately new Kilner jars in my supermarket
have plastic seals - so an experiment will be required.

Dave

-----Original Message-----
X-from: gunkelrl-at-slu.edu [mailto:gunkelrl-at-slu.edu]
Sent: 02 August 2005 17:49
To: David Patton

We use Osmium in our lab and keep it in the refrigerator.
We keep it in a glass bottle and cover the lid with
parafilm. Then we put that bottle in another bottle with a
screw top and wrap the bottle in foil. It works great, our
refrigerator doesn't have and black residue, just the two
bottles.

rebecca

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Dear Veselin, dear list members,

appart from the good explanations you already received from other list
members, I recommend you another link where you can also find explanations
on how the NA determines the microscope resolution:

http://support.svi.nl/wiki/NumericalAperture

As this is a wiki site, you can also share your knowledge by editing it!

Regards,

jose.

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7, 22 -- cc: sswaffe-at-abv.bg
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Hola

Homeowner

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Good Day,

Rachelle Mayo

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Dear Nestor,

will replies like these reach the sender? I mean, this question was posted
from AskAMicroscopist, not emailed by a list member, so I am afraid our
replies will not reach people like Veselin unless we explicity include
them in the address of our emails...

jose.

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Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html

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16, 17 -- From: "Skage Hem" {SHem-at-laurentian.ca}
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Hi, Skage

We have a consultant who has a lot of experience in this area and also provides training. I"ll forward your email to him

Best regards
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.

At 10:08 AM 8/3/2005, SHem-at-laurentian.ca wrote:

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Skage,

We have been running an older version, S200 for some 15 years. Overall, it
has had an excellent service history (mostly replacing vacuum pumps and
occasional chips, resistors, capacitors, etc).

We are a metallurgy lab, so our samples tend to be conductive and dry. The
large sample chamber is a real plus as is the nice stage. Also, we are not
heavy users and kept limited access with regard to number of users. We do
miss having direct digital imaging, which I presume the S250 has. Your
situation may be very different than ours. Newer scopes offer partial
vacuum capabilities and probably better ultimate resolution.

Other than being an older instrument (not always a bad thing) without
some of the newer bells and whistles, I have no regrets and we look forward
to many more years of service from it.

Alan Stone
ASTON

At 10:07 AM 8/3/2005, you wrote:

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Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com

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I had known at one time, but I suppose due to age, I can't remember, but I'm
hoping that people here could advise me as to the best knife angle to buy on
a Diatome knife. All our specimens are human biopsy tissue embedded in
epon. I'd like to buy a histo-knife, and I was under the impression that 45
degrees would be the best sort of knife angle for routine use like this. Is
this correct?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

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3, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
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In North America, Kilner jars are called Mason jars.

--
Lesley Weston

} From: David.Patton-at-uwe.ac.uk
} Reply-To: microscopy-at-microscopy.com
} Date: Wed, 03 Aug 2005 06:26:13 -0500
} To: leswes-at-shaw.ca
} Subject: [Microscopy] Osmium vapors
}
}
}
}
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} Osmium vapours are not stopped by simple bottle tops. We buy ours
} already made up. The bottle lids have a plastic (?) insert which is
} effective.
}
} Waste osmium is stored in any bottle which is then kept in what we can
} in the UK a Kilner jar. It is made of glass, has a rubber seal and uses
} wire to clamp the lid very tightly. Osmium vapours do not seem to pass
} through rubber seals. Unfortunately new Kilner jars in my supermarket
} have plastic seals - so an experiment will be required.
}
} Dave
}
} -----Original Message-----
} X-from: gunkelrl-at-slu.edu [mailto:gunkelrl-at-slu.edu]
} Sent: 02 August 2005 17:49
} To: David Patton
} Subject: [Microscopy] Re: Osmium vapors
}
}
}
}
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} We use Osmium in our lab and keep it in the refrigerator.
} We keep it in a glass bottle and cover the lid with
} parafilm. Then we put that bottle in another bottle with a
} screw top and wrap the bottle in foil. It works great, our
} refrigerator doesn't have and black residue, just the two
} bottles.
}
} rebecca
}
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I use a 45 for normal work. I find it works very well. I have a 35 I
use for special work. 35 has some advantages, but is not as robust.
David

On Aug 3, 2005, at 11:41 AM, GBurgess-at-exchange.hsc.mb.ca wrote:

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} I had known at one time, but I suppose due to age, I can't remember,
} but I'm
} hoping that people here could advise me as to the best knife angle to
} buy on
} a Diatome knife. All our specimens are human biopsy tissue embedded in
} epon. I'd like to buy a histo-knife, and I was under the impression
} that 45
} degrees would be the best sort of knife angle for routine use like
} this. Is
} this correct?
}
} This e-mail and/or any documents in this transmission is intended for
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5, 22 -- From Elliott-at-arizona.edu Wed Aug 3 13:58:43 2005
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it has been a while since i have purchased a knife. 48
degrees should work well for biological tissue.
if you have doubts, questions or concerns i recommend
contacting staci kirsh at electron microscopy
sciences, she is an invaluble resource.
john

--- GBurgess-at-exchange.hsc.mb.ca wrote:

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}
} I had known at one time, but I suppose due to age, I
} can't remember, but I'm
} hoping that people here could advise me as to the
} best knife angle to buy on
} a Diatome knife. All our specimens are human biopsy
} tissue embedded in
} epon. I'd like to buy a histo-knife, and I was
} under the impression that 45
} degrees would be the best sort of knife angle for
} routine use like this. Is
} this correct?
}
} This e-mail and/or any documents in this
} transmission is intended for the address(s) only and
} may contain legally privileged or confidential
} information. Any unauthorized use, disclosure,
} distribution, copying or dissemination is strictly
} prohibited. If you receive this transmission in
} error, please notify the sender immediately and
} return the original.
}
} ==============================Original
} Headers==============================
} 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Wed Aug 3
} 13:35:20 2005
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} 3, 20 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
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__________________________________________________
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I have had 45 degree diamonds for most of my career but last year bought a
35 degree one. I think there is a significant improvement for the lymphoid
tissue we are cutting these days. I hear they wear out faster but I don't
do a ton of sectioning so the tradeoff seems worth it. Tom

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

==============================Original Headers==============================
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I have received your message, but will be out of the office until August 4th and will not be able to reply until then.

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lambert-at-jeol.com wrote:

} I have received your message, but will be out of the office

I apologize to the list for the unnecessary messages. We have recently started using the automated reply feature of our email server with some unexpected results. Attempts to repair the auto-reply logic have (so far) failed. Hopefully, my unsubscribe request will be processed soon. Again, I apologize for wasted bandwidth.

Best regards,
Mike Lambert

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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html

==============================Original Headers==============================
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31, 23 -- From kenconverse-at-qualityimages.biz Wed Aug 3 17:04:21 2005
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31, 23 -- To: {microscopy-at-microscopy.com}
31, 23 -- Subject: RE: [Microscopy] Cambridge S-250
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Hi Ken, Its replacing a nanolab-7 (old Zeiss)
Skage

} } } kenconverse-at-qualityimages.biz 08/03/05 6:08 PM } } }

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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html

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31, 23 -- From kenconverse-at-qualityimages.biz Wed Aug 3 17:04:21 2005
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Contents Retrieved from Microscopy Listserver Archives
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Diatome suggest for their 'ultra' diamond knives, that 45 degree is
good for routine sections. 35 degree apparently offers less section
distortions in biology and materials science. 55 degree is best for
really hard ceramics and similar materials

The Diatome article offers two references:
1. J.C. Jesior; How to avoid compression; Jnl. of Ultrastructure &
Molecular Structure Res. 95, 210-217 (1986)
2. J.C. Jesior; Use of low-angle diamond knives leads to improved
ultrastructural preservation of ultrathin sections; Scanning Microscopy
Supplement 3, 147-153 (1989); Scanning Microscopy International,
Chicago (AMF O'Hare) IL 6066 USA.

This information came out of a Diatome colour brochure which I must
have received some time in the last 10 years or so. I do not have the
actual references.

NB Incidentally Diatome do market a 'Histo' knife but it is really
intended for light microscopy sections rather than ultrathin ones
('Ultra' knives).

Good luck

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
X-from: GBurgess-at-exchange.hsc.mb.ca

unless i have forgotten, diatome also/used to market a
semi diamone kife that would cut semi thin sections as
well as thin sections. of course the memory is the
first thing to go.

--- malcolm.haswell-at-sunderland.ac.uk wrote:

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} Diatome suggest for their 'ultra' diamond knives,
} that 45 degree is
} good for routine sections. 35 degree apparently
} offers less section
} distortions in biology and materials science. 55
} degree is best for
} really hard ceramics and similar materials
}
} The Diatome article offers two references:
} 1. J.C. Jesior; How to avoid compression; Jnl. of
} Ultrastructure &
} Molecular Structure Res. 95, 210-217 (1986)
} 2. J.C. Jesior; Use of low-angle diamond knives
} leads to improved
} ultrastructural preservation of ultrathin sections;
} Scanning Microscopy
} Supplement 3, 147-153 (1989); Scanning Microscopy
} International,
} Chicago (AMF O'Hare) IL 6066 USA.
}
} This information came out of a Diatome colour
} brochure which I must
} have received some time in the last 10 years or so.
} I do not have the
} actual references.
}
} NB Incidentally Diatome do market a 'Histo' knife
} but it is really
} intended for light microscopy sections rather than
} ultrathin ones
} ('Ultra' knives).
}
} Good luck
}
} Malcolm
}
} Malcolm Haswell
} e.m. unit
} School of Health, Natural and Social Sciences
} Fleming Building
} University of Sunderland
} Tyne & Wear
} SR1 3SD
} UK
} e-mail: malcolm.haswell-at-sunderland.ac.uk
}
}
} ----- Original Message -----
} X-from: GBurgess-at-exchange.hsc.mb.ca
} Date: Wednesday, August 3, 2005 7:41 pm
} Subject: [Microscopy] best knife angle
}
} }
} }
} }
} }
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} }
} } I had known at one time, but I suppose due to age,
} I can't
} } remember, but I'm
} } hoping that people here could advise me as to the
} best knife angle
} } to buy on
} } a Diatome knife. All our specimens are human
} biopsy tissue
} } embedded in
} } epon. I'd like to buy a histo-knife, and I was
} under the
} } impression that 45
} } degrees would be the best sort of knife angle for
} routine use like
} } this. Is
} } this correct?
} }
} } This e-mail and/or any documents in this
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} 2005
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} } 3, 20 -- From: Garry Burgess
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} Aug 4 03:39:20 2005
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Contents Retrieved from Microscopy Listserver Archives
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Skage,
I'm not really familiar with either model, but maybe someone on the list is
and can give you some recommendations. You might also try contacting Earl
Weltmer of Scanservice Corp.
earlw-at-sbcglobal.net
as he is familiar with more makes and models than I am.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: shem-at-laurentian.ca [mailto:shem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 8:44 PM
To: kenconverse-at-qualityimages.biz

Hi Ken, Its replacing a nanolab-7 (old Zeiss)
Skage

} } } kenconverse-at-qualityimages.biz 08/03/05 6:08 PM } } }

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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html

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16, 17 -- From: "Skage Hem" {SHem-at-laurentian.ca}
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31, 23 -- From kenconverse-at-qualityimages.biz Wed Aug 3 17:04:21 2005
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31, 23 -- Subject: RE: [Microscopy] Cambridge S-250
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53, 23 -- From kenconverse-at-qualityimages.biz Thu Aug 4 09:29:41 2005
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thanks for the info, mannie is still around and hasn't
retired yet. i just got an email from him. we went to
college together. i can't tell you the number of
friends and girl friends i lost touch with from
college. some i regret others i do not.
so are you heading to st martins?

--- microbill-at-mohawk.net wrote:

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} The last I knew he was at MD Anderson in Texas - but
} he may well have
} retired by now - see
} http://woodenwonderstx.com/About.html
}
}
}
}
} At 12:49 PM 8/2/2005, you wrote:
}
}
}
}
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} }
} } is mannie Mannie Steglich still around? haven't
} heard
} } from him in a while.
} } john
} }
} } --- hoffpajo-at-yahoo.com wrote:
} }
} } }
} } }
} } }
} } }
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} } }
} } } in the alternative and it may be more expensive
} and
} } } you may not want to go there. and if someone
} with a
} } } better memory than i do anymore, can correct me.
} i
} } } do
} } } remember seeing premade OsO4 in small amounts.
} use
} } } what you need, perhaps even batch the tissue
} } } samples.
} } } then throw out the unused in the waste.
} } } just a thought.
} } }
} } } --- dmclea-at-sandia.gov wrote:
} } }
} } } }
} } } }
} } } }
} } } }
} } }
}
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} } } }
} } } } Marc
} } } }
} } } } We store our osmium in a secondary
} container...a
} } } } large poly pro bottle
} } } } (capped) and then we bag that container in a
} } } plastic
} } } } Ziploc. It helps a
} } } } bit.
} } } }
} } } } Dorrance
} } } }
} } } } -----Original Message-----
} } } } X-from: marc.pypaert-at-yale.edu
} } } } [mailto:marc.pypaert-at-yale.edu]
} } } } Sent: Tuesday, August 02, 2005 9:10 AM
} } } } To: McLean, Dorrance
} } } } Subject: [Microscopy] Osmium vapors
} } } }
} } } }
} } } }
} } } }
} } } }
} } }
}
} ------------------------------------------------------------------------
} } } } ----
} } } } The Microscopy ListServer -- CoSponsor: The
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}
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} ------------------------------------------------------------------------
} } } } ----
} } } }
} } } } Question to the list:
} } } }
} } } } Our local safety inspector is concerned about
} the
} } } } appearance of the
} } } } fridge where we stock our solution of osmium.
} As
} } } you
} } } } can expect the
} } } } white interior of the fridge has turned grey
} with
} } } } vapors of osmium over
} } } } the years. Although we are taking every
} } } precautions
} } } } to avoid leakage of
} } } } osmium vapor from its container, this is a
} problem
} } } } that I have observed
} } } } in every EM lab that I have worked in. Does
} } } someone
} } } } on the list have a
} } } } method to store osmium in such a way as to
} avoid
} } } any
} } } } escape of vapor?
} } } } Also, is there something we could put into
} fridge
} } } } that would trap osmium
} } } } vapors as they leak out? Thanks for your
} advise.
} } } }
} } } } Marc
} } } }
} } } } --
} } } } Marc Pypaert
} } } } Department of Cell Biology
} } } } Center for Cell and Molecular Imaging
} } } } Ludwig Institute for Cancer Research
} } } } Yale University School of Medicine
} } } } 333 Cedar Street, PO Box 208002
} } } } New Haven, CT 06520-8002
} } } } TEL 203-785 3681
} } } } FAX 203-785 7446
} } } }
} } } }
} } } } ==============================Original
} } } } Headers==============================
} } } } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } } } 11:09:11 2005 5, 18 --
} } } } Received: from BIOMED.MED.YALE.EDU
} } } } (biomed.med.yale.edu
} } } } [130.132.232.48])
} } } } 5, 18 -- by ns.microscopy.com
} (8.12.11/8.12.8)
} } } with
} } } } ESMTP id
} } } } j72G9BnV011512
} } } } 5, 18 -- for {microscopy-at-microscopy.com} ;
} Tue, 2
} } } } Aug 2005
} } } } 11:09:11 -0500
} } } } 5, 18 -- Received: from yale.edu
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} } } } [130.132.234.111]) 5, 18 -- by
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} 2005
} } } } 12:09:29 -0400 (EDT)
}
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5, 19 -- From hoffpajo-at-yahoo.com Thu Aug 4 10:52:35 2005
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Fellow listers:

Just a reminder that the "Reply" functionality of the
list has recently changed.

Currently, when you "Reply" to a message, the e-mail
is sent to the entire list, not just the original
sender. I believe that this is/was a biproduct of
Nestor's efforts to minimize SPAM (great job Nestor,
keep up the good work).

This is different than in the past, when a "Reply"
only went to the author of the post you were replying
to.

While this makes it easier to reply and have a post go
to the group, it also seems to have spawned a number
of messages there were meant for specific individuals,
but made their way to the entire group.

Not necessarily a problem, but in some cases it could
be...

John W. Raffensperger, Jr.
Beaver Dam, Wisconsin, USofA

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I noticed the Tungsten Carbide knives in a catalog, and wondered if this
sort of knife would be at all suitable to cut 0.7 micron sections of Epon
embedding biological material. Currently we are using histo-diamond knives,
but it is my dream to be able to use a more durable, cheaper knife that
gives the same result. I was just wondering if anyone has tried this, and
what sort of result they might get with epon. Or is it a stupid idea?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

==============================Original Headers==============================
3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Thu Aug 4 12:07:31 2005
3, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122])
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3, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
3, 20 -- Subject: Tungsten Carbide Knives and Semi-thin Epon Sections
3, 20 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
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Although I am only familiar with so-called 'hard' materials microtomy
(metals, alloys, minerals, ceramic fibers and the like) the interesting
point about the knife angle in this area is that the 35 sections better for
all of the above materials in my lab, so the 45 knives gather dust. Since
wear is a function of the amount of sectioning as well as type of material,
I cannot comment on that aspect, but can note that we never had any damage
to the 35 except when we deliberately tried to do so on ~20 micron,
extremely hard amorphous alloys particles. (It was not pretty!).

The success of the lower angle is no fluke, as Helmut Gnaegi of Diatome has
gotten good sections of; 200 micron quartz particles, polysilicon/metal
layers on a single crystal silicon substrate, carbon fibers (but care had to
be used or nicks would occur), superconducting oxide and Ti implants in
bone. Phil Swab (with a coatings company in California) has sectioned many
optical glass coatings, and layers of boron nitride and artificial diamond
on single crystal silicon substrate. To the best of my knowledge, both of
these specialists had trouble with any other angles.

My theory is that, for most of the above examples, the knife edge only
initiates a crack in the fairly brittle material which is then 'wedged open'
across the block face by the angle of the knife, hence the reduced damage
with reduced angle (though section breakup does occur).

Finally, we did a bit of fooling around with histo knives a few years back.
Far from being only good for semithins, though 1 micron thick sections of
aluminum were produced, they produced some of the thinnest, flattest
sections we ever obtained (~10 nm for nanocrystalline metals) and was the
only knife that produced decent ~20 nm thick sections of the above amorphous
alloy particles. Go figure.

Tom

Dr. Tom Malis
Manager / Gestionnaire
Academic User Access Facility (AUAF) / La Facilit d’accs aux utilisateurs
universitaire (FAUU)
CANMET Materials Technology Laboratory / LTM-CANMET
Natural Resources Canada / Ressources naturelles Canada
568 Booth St. / 568 rue Booth, Ottawa, Ontario K1A 0G1
Tel.: (613) 995-1493 FAX: (613) 992-8735; cell: 613-371-4577
malis-at-nrcan.gc.ca

-----Original Message-----
X-from: phillipst-at-missouri.edu [mailto:phillipst-at-missouri.edu]
Sent: August 3, 2005 3:38 PM
To: malis-at-nrcan.gc.ca

I have had 45 degree diamonds for most of my career but last year bought a
35 degree one. I think there is a significant improvement for the lymphoid
tissue we are cutting these days. I hear they wear out faster but I don't
do a ton of sectioning so the tradeoff seems worth it. Tom

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu

==============================Original Headers==============================
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the histo knife should last for years in the hands of
a careful tech. and if you are using the sections for
orientation a few knife scratches should matter. just
be sure to not let an inexperienced person use them.

--- GBurgess-at-exchange.hsc.mb.ca wrote:

}
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} I noticed the Tungsten Carbide knives in a catalog,
} and wondered if this
} sort of knife would be at all suitable to cut 0.7
} micron sections of Epon
} embedding biological material. Currently we are
} using histo-diamond knives,
} but it is my dream to be able to use a more durable,
} cheaper knife that
} gives the same result. I was just wondering if
} anyone has tried this, and
} what sort of result they might get with epon. Or is
} it a stupid idea?
}
} This e-mail and/or any documents in this
} transmission is intended for the address(s) only and
} may contain legally privileged or confidential
} information. Any unauthorized use, disclosure,
} distribution, copying or dissemination is strictly
} prohibited. If you receive this transmission in
} error, please notify the sender immediately and
} return the original.
}
} ==============================Original
} Headers==============================
} 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Thu Aug 4
} 12:07:31 2005
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} 3, 20 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
} 3, 20 -- Subject: Tungsten Carbide Knives and
} Semi-thin Epon Sections
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I have used the Tungsten Carbide knives some. They are not sharp enough to
cut sub-micron thick sections as they leave a slight snowy appearance to the
block face. I have been successful in cutting plastic embedded tissue
sections of approximately 3-5 microns with them. The edge stays good much
longer than glass does. They are great for working with harder materials.
After applying per-mount and coversliping the sections, the snowiness is no
longer evident.

===========================================
Michael D. Standing
Microscopy Technician
Brigham Young University Microscopy Lab
A-125A CLFB
Provo, UT 84602

Phone: (801) 422-4011
E-Mail: Michael_Standing-at-byu.edu
===========================================

-----Original Message-----
X-from: GBurgess-at-exchange.hsc.mb.ca [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Thursday, August 04, 2005 11:09 AM
To: Michael_Standing-at-byu.edu

I noticed the Tungsten Carbide knives in a catalog, and wondered if this
sort of knife would be at all suitable to cut 0.7 micron sections of Epon
embedding biological material. Currently we are using histo-diamond knives,
but it is my dream to be able to use a more durable, cheaper knife that
gives the same result. I was just wondering if anyone has tried this, and
what sort of result they might get with epon. Or is it a stupid idea?

This e-mail and/or any documents in this transmission is intended for the
address(s) only and may contain legally privileged or confidential
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3, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
3, 20 -- Subject: Tungsten Carbide Knives and Semi-thin Epon Sections
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12, 21 -- From Michael_Standing-at-byu.edu Thu Aug 4 12:58:57 2005
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12, 21 -- From: "Michael Standing" {Michael_Standing-at-byu.edu}
12, 21 -- To: {microscopy-at-microscopy.com}
12, 21 -- Subject: RE: [Microscopy] Tungsten Carbide Knives and Semi-thin Epon Sections
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Another couple reminder about recent changes-

First, it used to be that a poster's personal name would appear in the
X-from: field and give a pretty good idea of their identity. (List rules
(http://www.msa.microscopy.org/MicroscopyListserver/Rules.html) ask that we
provide our real name as part of the post.) Recent changes have stripped
that field down to just the e-mail address. That makes messages look a lot
more anonymous than before. In fact, the original From: field is preserved
in the new stuff tacked on to the bottom of the post, but many of us
probably don't look those lines over. So, it is probably a good practice to
add a brief signature line so we clearly know who you are and can avoid
some of the recent confusion.

Second, there are those new lines of stuff tacked onto the end of the
posts. I encourage you all to trim those back as you reply to messages.
They add unnecessary bulk to the messages. A new set is always tacked on
anyway.

Cheers, even to those of you in Hawaii. I almost joined you there.

Warren Straszheim
Iowa State University

At 11:11 AM 08/04/05, you wrote:

} Fellow listers:
}
} Just a reminder that the "Reply" functionality of the
} list has recently changed.
}
} Currently, when you "Reply" to a message, the e-mail
} is sent to the entire list, not just the original
} sender. I believe that this is/was a biproduct of
} Nestor's efforts to minimize SPAM (great job Nestor,
} keep up the good work).
}
} This is different than in the past, when a "Reply"
} only went to the author of the post you were replying
} to.
}
} While this makes it easier to reply and have a post go
} to the group, it also seems to have spawned a number
} of messages there were meant for specific individuals,
} but made their way to the entire group.
}
} Not necessarily a problem, but in some cases it could
} be...
}
} John W. Raffensperger, Jr.
} Beaver Dam, Wisconsin, USofA

==============================Original Headers==============================
11, 20 -- From wesaia-at-iastate.edu Thu Aug 4 13:09:47 2005
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On Aug 4, 2005, at 1:03 PM, Michael_Standing-at-byu.edu wrote:

}
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}
} I have used the Tungsten Carbide knives some. They are not sharp
} enough to
} cut sub-micron thick sections as they leave a slight snowy
} appearance to the
} block face. I have been successful in cutting plastic embedded tissue
} sections of approximately 3-5 microns with them. The edge stays
} good much
} longer than glass does. They are great for working with harder
} materials.
} After applying per-mount and coversliping the sections, the
} snowiness is no
} longer evident.
}
} ===========================================
} Michael D. Standing
} Microscopy Technician
} Brigham Young University Microscopy Lab
} A-125A CLFB
} Provo, UT 84602
}
} Phone: (801) 422-4011
} E-Mail: Michael_Standing-at-byu.edu

Exactly, Michael.

The problem with tungsten carbide is that it is actually a
microcrystalline alloy of tungsten carbide particles bonded with
cobalt, and this microstructure structure interferes with very thin
sections, much as with steel, but the carbide is harder and tougher,
which is why it is used to machine steel.

Knife facets may look quite shiny but they need to be polished much
flatter than a wavelength of visible light if they are to meet at a
smooth edge measured in the low nanometers, and thus be capable of
cutting the sub-100 nanometer sections appropriate for EM work.

The good thing about metal alloys is that the edge angle can be made
more acute while retaining the desired durability, but only at the
expense of minimum section thickness, so they are used for LM work.

Glass does well for EM knives because it is amorphous and thus has no
microstructure, while being hard enough to section. Of the hard
monocrystal alternatives to glass suitable for EM knives, only
sapphire, silicon carbide and diamond seem appropriate. All these are
good candidate materials for ultramicrotome knives, depending on cost
and other requirements.

Roger Baker
1303 Bentwood,
Austin, Tx,
78722

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12, 19 -- Subject: Re: [Microscopy] RE: Tungsten Carbide Knives and Semi-thin Epon Sections
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Greetings,
I am looking for a tech that used to work here at UMBC, his name is
Phil Rutledge. If anyone knows his whereabouts could you have him e-mail
me at cpetty1-at-umbc.edu. He is a whiz with both our zeiss and jeol and I
would love to ask him some questions about the scopes and stuff I have
found lying about the facility.
Thanks
--
Chere Petty, M.S.
Manager, Keith R. Porter Imaging Facility
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
1000 Hilltop Circle
Baltimore, MD 21250
Phone: 410-455-2296
Fax: 410-455-3875

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Glass Knives, cheap fast, reliable, Histo Diamonds are excellent and durable
if used with care!
Markus F. Meyenhofer
Microscopy Labs.
----- Original Message -----
X-from: {GBurgess-at-exchange.hsc.mb.ca}
To: {micro-at-superlink.net}
Sent: Thursday, August 04, 2005 1:07 PM

yes i agree, glass knives are relativly cheap, fast?
depends on what you are using them for. histo knives
should last for years and can be used to face the
blocks as well. used one for years untill someone with
little experience got hold of it, i had never seen a
diamond with so many chips in it.
moral of the story, keep your knives close, but keep
your diamind knives to yourself.

--- micro-at-superlink.net wrote:

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}
} Glass Knives, cheap fast, reliable, Histo Diamonds
} are excellent and durable
} if used with care!
} Markus F. Meyenhofer
} Microscopy Labs.
} ----- Original Message -----
} X-from: {GBurgess-at-exchange.hsc.mb.ca}
} To: {micro-at-superlink.net}
} Sent: Thursday, August 04, 2005 1:07 PM
} Subject: [Microscopy] Tungsten Carbide Knives and
} Semi-thin Epon Sections
}
}
} }
} }
} }
} }
}
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} }
} }
} } I noticed the Tungsten Carbide knives in a
} catalog, and wondered if this
} } sort of knife would be at all suitable to cut 0.7
} micron sections of Epon
} } embedding biological material. Currently we are
} using histo-diamond
} } knives,
} } but it is my dream to be able to use a more
} durable, cheaper knife that
} } gives the same result. I was just wondering if
} anyone has tried this, and
} } what sort of result they might get with epon. Or
} is it a stupid idea?
} }
} } This e-mail and/or any documents in this
} transmission is intended for the
} } address(s) only and may contain legally privileged
} or confidential
} } information. Any unauthorized use, disclosure,
} distribution, copying or
} } dissemination is strictly prohibited. If you
} receive this transmission in
} } error, please notify the sender immediately and
} return the original.
} }
} } ==============================Original
} } Headers==============================
} } 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Thu Aug
} 4 12:07:31 2005
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} } 3, 20 -- Date: Thu, 4 Aug 2005 12:06:02 -0500
} } 3, 20 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
} } 3, 20 -- Subject: Tungsten Carbide Knives and
} Semi-thin Epon Sections
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} 20:14:33 2005
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We use bottles with rubber septums to store the liquid, and keep the
bottles within sealed falcon tubes, within a sealed bottle. This keeps
the vapours from leaking and discolouring the fridge.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Tue, 2 Aug 2005, marc.pypaert-at-yale.edu wrote:

}
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} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
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} 5, 18 -- Subject: Osmium vapors
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4, 26 -- Date: Fri, 5 Aug 2005 10:35:39 -0700 (PDT)
4, 26 -- From: Gordon Vrololjak {gvrdolja-at-nature.berkeley.edu}
4, 26 -- To: microscopy-at-microscopy.com
4, 26 -- Subject: Re: [Microscopy] Osmium vapors
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Dear Listservers,

The Penn Regional Nanotechnology Facility at the University of
Pennsylvania has an opening for a Research Scientist/Microscopist. The
facility is equipped with transmission and scanning electron microscopes,
atomic force microscopes, an ion accelerator and a focused ion beam. The
successful candidate will assist the facility's technical director in
training and assisting users, developing methods and maintaining the
instruments.
Qualifications for this position include an M.S. in materials
or other physical science (Ph.D. preferred). A minimum of three years
experience in the operation of SEM, TEM, FIB or AFM (experience with FIB or
AFM preferred).

For immediate consideration, send resume/cv to:

Doug Yates
dmyates-at-lrsm.upenn.edu
Penn Regional Nanotechnology Facility
University of Pennsylvania
3231 Walnut Street
Philadelphia, PA 19104

Additional information about the position may be viewed at:
http://www.hr.upenn.edu/jobs
reference number: 050818006

Information about the Penn Regional Nanotechnology Facility may be viewed at:
http://www.seas.upenn.edu/nanotechfacility

Thanks,
Doug Yates

==============================Original Headers==============================
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7, 28 -- Date: Fri, 5 Aug 2005 16:58:18 -0400
7, 28 -- From: dmyates-at-seas.upenn.edu
7, 28 -- To: Microscopy-at-microscopy.com
7, 28 -- Subject: Microscopist Position Available - Univ Penn
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==============================Original Headers==============================
2, 21 -- From schenderson-at-vcu.edu Fri Aug 5 17:59:29 2005
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2, 21 -- Reply-To: {schenderson-at-vcu.edu}
2, 21 -- From: "Scott Henderson" {schenderson-at-vcu.edu}
2, 21 -- To: {Microscopy-at-microscopy.com}
2, 21 -- Subject: Microscopy Technician position available
2, 21 -- Date: Fri, 5 Aug 2005 18:59:35 -0400
2, 21 -- Organization: Virginia Commonwealth University
2, 21 -- MIME-Version: 1.0
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The following technical position is available at Virginia Commonwealth
University School of Medicine.

Microscopy Technician (Position # 55122) �

A technical position is available in the Microscopy Facility of the
Department of Anatomy and Neurobiology in the School of Medicine at Virginia
Commonwealth University. The facility houses confocal, multi-photon,
fluorescence, and electron microscopes (TEM & SEM).� The successful
candidate will assist with microscopy studies of various biological
systems.� Duties include instructing and assisting users of the facility,
sample preparation, image analysis and minor equipment maintenance.�
Applicants should have excellent communication and organizational skills, an
understanding of basic laboratory procedures, and the ability to manage a
large and varied workload. Qualifications include a degree in Biology/Life
Sciences, at least 2 years of experience with laser scanning microscopy
(confocal and/or multi-photon), sample preparation, digital imaging, and
image analysis.� Experience with electron microscopy is an asset. Computer
skills are essential.�

Applications are to be submitted online via the VCU Jobs website at:

www.vcujobs.com

Click on the �Search Postings� link and under the �Working Title� field,
select �Microscopy Technician�

--------------------------

Scott Henderson, Ph.D.
Director of Microscopy
Associate Professor
Dept. Anatomy & Neurobiology
Virginia Commonwealth University
School of Medicine
Sanger Hall, Rm. 9-069d
1101 East Marshall St.
Richmond, VA 23298-0709

==============================Original Headers==============================
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9, 22 -- Reply-To: {schenderson-at-vcu.edu}
9, 22 -- From: "Scott Henderson" {schenderson-at-vcu.edu}
9, 22 -- To: {Microscopy-at-microscopy.com}
9, 22 -- Subject: Microscopy Technician position available
9, 22 -- Date: Fri, 5 Aug 2005 19:08:06 -0400
9, 22 -- Organization: Virginia Commonwealth University
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ken.blight-at-cancer.org.uk) from
http://microscopy.com/MLFormMail.html on Wednesday, August 3, 2005 at
10:40:02
---------------------------------------------------------------------------

Email: ken.blight-at-cancer.org.uk
Name: Ken Blight

Organization: cancer research uk

Title-Subject: [Filtered] Drosophila eye ultrathin cryosections

Question: I have processed Drosophila eyes for immunolabelling (EM)
using the Tokuyasu technique and the results are, to say the least,
very disappointing. The eyes floated in the fixative which didn't
help matters. The problem looks to be spacial when I compare the
results to standard resin TEM preparation. Maybe there is some
problem with buffers, fixation or section expansion. Does anyone
have experience of these tough little beasties?

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:33:05 2005
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6, 12 -- Date: Fri, 5 Aug 2005 19:33:05 -0500
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: ken.blight-at-cancer.org.uk (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Drosophila eye ultrathin cryosections
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cornheadorama-at-hotmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, August 3, 2005 at 18:07:30
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver: Re: Cambridge S250

Question: Hi,

I'm not too familiar with these despite the fact I have two of them
gathering dust. But I have heard them disfavorably compared to the
S200. Mine may be site-rigged for handling semi wafers (and there's a
chance both came from the same facility, at different times... a
hunch) but in mine the stage uses little more substantial than pvc
tubing to transmit the z-axis knob to the stage: horrible hysteresis.

I suspect these and related scopes were enormously popular, and I
know for a fact this 'scope has die-hard fans.

LaB6 is typical but it wouldn't surprise me if there was an active
aftermarket upgrade to FE: indeed one of mine may have one. On the
other hand, you may have missed the bulk of the aftermarket boat by
about 5 years: lots of parts for old scopes falling off of price
lists.... but sometimes onto "clearance" lists :)

But I'd say even if your own scope leaves a lot to be desired,
trading it for another old scope like this? I'd say deosn't quite
make sense unless you have a special requirement. Ultimately, it just
may not be worth the shipping: which of course is dicey unless
there's at least a few hours teardown and re-assembly. Better the
devil you know.

I hope more people respond, I've been back and forth as to whether to
try to get one or both of mine working for about 6 years now :)

-Jeff

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==============================Original Headers==============================
13, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:33:27 2005
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cornheadorama-at-hotmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, August 3, 2005 at 18:41:52
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver:b Re: Osmium Vapors

Question: I don't have experience with osmium compounds, and my
suggestion may not be very practical (read: expensive) unless you
have piles of vacuum fittings lying around like I do, but on occasion
when wanting to "absolutely" seal something up I've used a KF50
stainless nipple (or bellows, sometimes)of appropriate length, a pair
of stainless blanks, clamps, o-rings etc. so that the item could be
sealed "vacuum tight". Of course it's all relative but I wouldn't
think much vapour will diffuse though the ~5mm of highly compressed
Viton. The clamps are a bit clumsy to put on but very quick to come
off: I'm not sure you can get a quick-release clamp in KF50 but that
would make it almost one-handed. You could probably save money and
thru-gassing by having a single-ended arrangment, ie: a pipe section
with a KF50 flange welded on one end and a cap welded on the other.
Some (if not most) of the hi-vac houses weld their standard catalog
items on-site, and some don't charge much extra (~%20 premium) for
custom items from stock parts.

I think the idea of absorbants/reactants holds a lot of potential.
Activated charcoal comes immediately to mind.
Is it so reactive as to present a fire hazard if quickly adsorbed
onto the huge surface area?

-Jeff

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==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:33:55 2005
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8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Osmium Vapors
8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (omelon-at-geology.utoronto.ca) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Thursday, August 4, 2005 at 16:57:53
---------------------------------------------------------------------------

Email: omelon-at-geology.utoronto.ca
Name: Christopher R. Omelon

Organization: University of Toronto

Title-Subject: [Filtered] further hardening of LR White

Question: I have tried embedding large samples (~ 1cm3) of porous
rock material in a variety of resins and find that LR White (Hard)
provides the best infilling due to its low viscosity. That being
said, the blocks are not as hard as when using epoxide resins such as
EMBED 812. I am cold-curing (i.e. with accelerator) at both room
temperature and at 4?C with mixed results, but even in the best case
the blocks are not as hard as I would expect. My question: can I
further harden the blocks by now placing these samples in the oven at
60?C? My reasoning is that resin within the block (i.e. beneath the
surface and therefore not exposed to oxygen) will proceed with
polymerization at a faster rate than if I left the blocks at room
temperature. Or is the reaction now at completion? Thanks

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==============================Original Headers==============================
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6, 12 -- Date: Fri, 5 Aug 2005 19:34:17 -0500
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: omelon-at-geology.utoronto.ca (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: further hardening of LR White
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (block-at-nova.edu) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
August 5, 2005 at 09:44:34
---------------------------------------------------------------------------

Email: block-at-nova.edu
Name: R. Block

Organization: NSU

Title-Subject: [Filtered] MListserver:

Question: Can you give me a source of information explaining pseudo-
confocal microscopy, how the data are acquired and analyzed?
Thanks in advance.

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:35:01 2005
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: block-at-nova.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: pseudo-confocal microscopy
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (elke.buschbeck-at-uc.edu) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, August 5, 2005 at 12:04:08
---------------------------------------------------------------------------

Email: elke.buschbeck-at-uc.edu
Name: Elke

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:Sodium Azide and TEM

Question: Does anyone know if it is o.k. to use sodium azide with TEM
preps? Does it wash out o.k. or will it leave a nasty deposit?
Thanks

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:35:20 2005
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: elke.buschbeck-at-uc.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Sodium Azide and TEM
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sdmegs09-at-yahoo.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
August 5, 2005 at 13:04:14
---------------------------------------------------------------------------

Email: sdmegs09-at-yahoo.com
Name: Meghan Donahue

Organization: University of San Diego

Title-Subject: [Filtered] MListserver: Energy dispersive spectroscopy fixation

Question: I am trying to fix plant material for EDS in the TEM and I
cannot find literature stating the fixation techniques. Is fixation
different for EDS?

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:35:46 2005
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: sdmegs09-at-yahoo.com (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Energy dispersive spectroscopy fixation
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lauriern-at-videotron.ca) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 2, 2005 at 18:43:47
---------------------------------------------------------------------------

Email: lauriern-at-videotron.ca
Name: Norm

Organization: None

Education: Graduate College

Location: Canada

Question: What is the maximum resolution achievable in Phase Contrast
and also Interferential Contrat DIC microscopy.
Since in bright field it is appr 1.40 I am sure that because of the
phase rings as well as the use of Wollaston prism in DIC, it must be
much lower even using oil immersion objective.

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:36:15 2005
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Elka,
We use it routinely in buffers used to make up solutions for
immunolocalization on sections. I have never had a problem with
percipitation from sodium azide.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 8/5/05 7:41 PM, "elke.buschbeck-at-uc.edu" {elke.buschbeck-at-uc.edu} wrote:

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} Email: elke.buschbeck-at-uc.edu
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}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
}
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One concern I have always heard was the formation of metal azide,
especially lead azide. These explosive and unstable have been found in
older metal plumbing. When I started as a QC chemist years ago, dilute
concentrations of sodium azoide was used as a perservative in some test
solutions used in clinical chemistry. One than one person got an
unpleasent surprize when working with the older style lead drain pipes. I
later wanted to use a test solution for sulfur which contained sodium
azide, my management had fits. I never did use that test.

I don't want to creat an electron storm of words, but I will suggest you
check out disposial method for your own safety.

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

330-668-2235 Ext. 238

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elke.buschbeck-at-uc
.edu To: frank.karl-at-degussa.com
cc:
08/05/2005 08:38 Subject: [Microscopy] viaWWW: Sodium Azide and TEM
PM
Please respond to
microscopy

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Title-Subject: [Filtered] MListserver:Sodium Azide and TEM

Question: Does anyone know if it is o.k. to use sodium azide with TEM
preps? Does it wash out o.k. or will it leave a nasty deposit?
Thanks

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Dear Microscopists,

We are looking to hire an Associate Scientist in our Bioimaging Center.
Although we place special emphasis on scanning probe microscopy, skills
in other areas of microscopy would be highly desirable. Please see the
job announcement below for additional details.

Best Regards, Kirk

*Associate Scientist � Bioimaging*

*University of Delaware*

*Delaware Biotechnology Institute*

The Delaware Biotechnology Institute (DBI) was established in 1999 as an
academic unit of the University of Delaware to position Delaware as a
leader in the life sciences. The Institute�s mission is to engage in
leading-edge scientific discovery in the life sciences, provide
biotechnology-based education, and promote economic development. The
major interdisciplinary research areas include human health,
agriculture, marine ecosystems and biomaterials.

The Institute functions as a partnership involving State government, the
Delaware institutions of higher education, and area industry, and is
housed in a new 72,000 ft2 state-of-the-art research facility. In
addition to individual research laboratories, it houses several core
facilities including a custom microarray center, a bioinformatics center
and a bioimaging center. The bioimaging center provides an array of
microscopy expertise and equipment, including conventional fluorescence,
confocal, multiphoton, atomic force, laser microdissection, transmission
and field emission scanning microscopes and ancillary sample preparation
equipment.

Under the limited direction of the Director of the Bioimaging Center,
the Associate Scientist independently interprets, organizes, executes,
and coordinates research assignments. Formulates and conducts research
on problems of considerable scope and complexity.

MAJOR RESPONSIBILITIES:

Provide consultation, training and supervision of graduate students,
post-doctoral fellows, staff and faculty in the design, implementation
and execution of microscopy-related projects with special emphasis on
scanning probe microscopy.

Interpret, organize, execute, and coordinate scientific research
assignments concerned with unique and highly complex problems.

Develop competitive research proposals to successfully generate external
research funding.

Collaborate with users and principal investigators on design, analysis,
application, and reporting of research projects; teach and advise on
techniques.

Design, perform, and/or oversee experiments, collect, analyze, and
interpret data, ensure data integrity, quality control, and protocol
compliance, prepare statistical and narrative reports and/or graphs.

Perform research assignments involving a number of variables, apply
diversified knowledge of scientific research principles, practices, and
protocols in research projects; make recommendations and conclusions
which serve as the basis for decision making.

Make authoritative decisions and recommendations that have a major
impact on scientific research activities and result in national and/or
international recognition.

Evaluate, select, and apply standard scientific techniques, procedures,
and criteria to accomplish a variety of research assignments.

Maintain a broad knowledge of state-of-the-art technology, software,
and/or systems.

Perform miscellaneous job-related duties as assigned

QUALIFICATIONS:

Master�s degree, Ph.D. preferred, in Biology/Chemistry or related field
as well as a record of peer reviewed publications and at least 5 years
demonstrated experience in scanning probe microscopy. Knowledge of
scientific approach, methodologies and scientific research principles,
practices and protocols in order to design, organize and coordinate
scientific research projects. Ability to perform independent original
research in an advanced area of scientific expertise. Ability to develop
scientific reports, proposals and publications on original research and
a knowledge of contemporary technological developments in the area of
scanning probe microscopy. Knowledge of the use and maintenance of
laboratory facilities and/or equipment. Ability to use independent
judgment to adapt and modify research concepts and approaches to
specific projects. Knowledge of current technological
developments/trends in area of scanning probe microscopy. Strong
communication, personal and organizational skills. Motivation to
learn/develop new techniques, flexibility, and ability to interact with
a diverse group of research personnel. approaches to specific projects.
Knowledge of current technological developments/trends in area of
scanning probe microscopy. Strong communication, personal and
organizational skills. Motivation to learn/develop new techniques,
flexibility, and ability to interact with a diverse group of research
personnel.

*Contact:* Interested candidates should forward curriculum vitae and the
names and contact information for three references to Dr. Kirk Czymmek,
15 Innovation Way, Suite 117, Delaware Biotechnology Institute,
University of Delaware, Newark, DE 19711 or by email (kirk-at-udel.edu).

Details can also be found at the following website:
http://www.dbi.udel.edu/career.html

==============================Original Headers==============================
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On Aug 5, 2005, at 5:36 PM, sdmegs09-at-yahoo.com wrote:

} Question: I am trying to fix plant material for EDS in the TEM and I
} cannot find literature stating the fixation techniques. Is fixation
} different for EDS?
}
Dear Meghan,
The preparation methods for any sample on which you are doing EDS or
other analytic techniques must take into account the elements you are
trying to analyze, so, for example, if you are looking for
water-soluble elements like Na or Cl ion, do not rinse the tissue in
solvents that will remove them. Note, however, that if you are looking
for Cl bound in organic compounds, such as polychlorinated biphenyls,
washing out the ion will enable you to distinguish the organochlorine.
For accurate quantitation, it is a good idea, if possible, to examine
the material untreated, except for freezing and perhaps sectioning,
then dehydrate by lyophylization in the scope and redo the analysis.
This will give accurate values for volatile elements and you can
improve the quantitation by taking the wet/dry ratios from elements
that are not affected by the drying process. Another consideration,
for the case that the elements of interest are not affected by your
preparation process, is not to use a stain that interferes with lines
from those elements. Pb and S are notorious for this, and one case I
ran into was trying to analyze Pt and Ir in a specimen that was treated
with Os and given to me. Since your specimens may not be suitable for
observation in the TEM when not prepared in some way, and since you may
not have access to cryopreparation methods, you need to keep in mind
the general principles that, whatever the preparation steps, they
cannot affect the concentration of the elements of interest, and cannot
interfere with the lines you want to measure. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

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A few weeks ago someone asked about a beam stop for a TEM.

Sopme time ago I designed a combination beam stop and Faraday cup for
a JEOL 2010 TEM. It has been in use for several years now, and I
understand has performed satisfactorily. If anyone is interested in
making use of the design, contact me.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-662-5237
Address mail to: 1136 Mixtwood Rd.
Ann Arbor, MI 48103-3035

==============================Original Headers==============================
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3, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu}
3, 14 -- Subject: [Microscopy]TEM: RE: Beam stop & Faraday cup
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and a terrific boss!
:)

----- Original Message -----
X-from: schenderson-at-vcu.edu

Dr. Bigelow,
I would love to have use of the design for our 2010. Also, do you think it
could be altered to fit a 1200 EX-II?

-----Original Message-----
X-from: bigelow-at-engin.umich.edu [mailto:bigelow-at-engin.umich.edu]
Sent: Monday, August 08, 2005 11:58 AM
To: jfb-at-uidaho.edu

A few weeks ago someone asked about a beam stop for a TEM.

Sopme time ago I designed a combination beam stop and Faraday cup for
a JEOL 2010 TEM. It has been in use for several years now, and I
understand has performed satisfactorily. If anyone is interested in
making use of the design, contact me.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-662-5237
Address mail to: 1136 Mixtwood Rd.
Ann Arbor, MI 48103-3035

==============================Original Headers==============================
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3, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu}
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Hi:

A guy came into the lab today asking me to image and measure some holes in
a silicon nitride wafer.

OK, I say, how big?

2 nm he says, some maybe up to 70 nm.

That's pretty small, I said. How did you plan on doing it?

Don't know, that's why I came to you, says he.

Here is what he's got. A chip about 1 mm thick with an FIB section out of
it that is like an inverted pyramid into the chip. The pyramid doesn't go
all the way through, but there is a thin area at the bottom that is
supposed to have the tiny hole. The chip is opaque in the TEM, way too
thick, the thin area is translucent at 80KV so we can sort of see where we
are going.

We rigged up a special TEM holder so we could put the chip in the TEM, but
searching for the FIB hole, then the tiny hole inside that took a while.
Eventually I could see something that looked like a hole and it was about
100 nm across. He said that was OK, it was a test hole and should be about
100 nm.

Finding this 100 nm hole was no picnic and I anticipate finding and
measuring a 2 nm hole to be harder. He wants to be able to pop one in and
check the diameter and pop in another one.

Anyone with some bright ideas about how to help this guy? We brainstormed
things like FESEM, AFM, some kind of diffraction with lasers, don't know if
any of them will work, don't have any more ideas.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu

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I've seen it as a constituent of a TEM fixative, so it shouldn't be a
problem. But why bother?

Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
}
}
} Email: elke.buschbeck-at-uc.edu
} Name: Elke
}
} Organization: University of Cincinnati
}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
}

==============================Original Headers==============================
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Any idea if there will be a single hole per sample or multiple holes? Will
you have any idea what the probable depth of the small hole will be (not
including the thinning they are apparently doing to the substrate)? The
hole's aspect ratio will have a lot to do with any analysis since a large
ratio (small diameter hole, long bore) can make alignment with any imaging
system a big problem.

This is probably a good time to jump in and see if they can't adjust their
experiment a little. Too often researchers jump before they can walk, and
assume that an existing method can provide whatever information they need,
without providing any compliance to existing techniques. A thinner
substrate would require less preliminary FIB thinning on their part and if
it were thin enough, could allow you to determine and measure the presence
of a hole even if the bore weren't perfectly aligned with the beam.

While they may be reluctant to discuss the work they are doing, details
aren't really needed. It may be in their best interest to understand the
hole formation process before extending it to such a thick substrate that
requires multiple operations. Unfortunately, it may be up to you to point
that out.

Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
Saint Charles, Illinois 60174
phone (630) 513-7093 fax (630) 513-7092
email mailto:ars-at-sem.com web www.sem.com

On Monday, August 08, 2005 5:51 PM, jmkrupp-at-cats.ucsc.edu
[SMTP:jmkrupp-at-cats.ucsc.edu] wrote:
}
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}
} Hi:
}
} A guy came into the lab today asking me to image and measure some holes
in
} a silicon nitride wafer.
}
} OK, I say, how big?
}
} 2 nm he says, some maybe up to 70 nm.
}
} That's pretty small, I said. How did you plan on doing it?
}
} Don't know, that's why I came to you, says he.
}
} Here is what he's got. A chip about 1 mm thick with an FIB section out of
} it that is like an inverted pyramid into the chip. The pyramid doesn't go
} all the way through, but there is a thin area at the bottom that is
} supposed to have the tiny hole. The chip is opaque in the TEM, way too
} thick, the thin area is translucent at 80KV so we can sort of see where
we
} are going.
}
} We rigged up a special TEM holder so we could put the chip in the TEM,
but
} searching for the FIB hole, then the tiny hole inside that took a while.
} Eventually I could see something that looked like a hole and it was about
} 100 nm across. He said that was OK, it was a test hole and should be
about
} 100 nm.
}
} Finding this 100 nm hole was no picnic and I anticipate finding and
} measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} check the diameter and pop in another one.
}
} Anyone with some bright ideas about how to help this guy? We brainstormed
} things like FESEM, AFM, some kind of diffraction with lasers, don't know
if
} any of them will work, don't have any more ideas.
}
} Thanks
}
} Jon
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
} ==============================Original
Headers==============================
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==============================Original Headers==============================
7, 20 -- From ars-at-sem.com Tue Aug 9 00:20:36 2005
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I don't know your exact problem, but some names of people who have
worked with tissues and elemental analysis:

P. Echlin
H. Plattner
A. Somlyo
RA Steinbrecht and K. Zierold

There are many more - sorry for not mentioning these names.

best regards,
RR

-------------------------------
PD Dr.Reinhard Rachel
Universit�t Regensburg
Lehrstuhl f�r Mikrobiologie
Universitaetsstr. 31
D-93053 Regensburg
tel.: +49 941 943 4534
fax.: +49 941 943 1824

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I will be in Peru from the 11th Aug to 20 Aug.
I will have a delayed time acces to emails.
For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
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For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
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==============================Original Headers==============================
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I don't care
Chris

----- Original Message -----
X-from: {luc-at-anaspec.co.za}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Tuesday, August 09, 2005 8:22 AM

I will be in Peru from the 11th Aug to 20 Aug.
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Hi George
Did you ever get the schematics for the ISI 100B that you were looking for?
Cheers
Peter Earl
Toronto

==============================Original Headers==============================
2, 26 -- From pwje-at-sympatico.ca Tue Aug 9 08:05:18 2005
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2, 26 -- Subject: Re: [Microscopy] AskAMicroscopist: schematics for an International Scientific
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Dear Dr.Henderson,

I noticed with interest your recent employment note on the Microscopy
listserver and hope that you are having good success.

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schenderson-at-vcu.edu wrote:

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13, 20 -- From randerson20-at-tampabay.rr.com Tue Aug 9 08:43:17 2005
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Why use sodium azide?

We are planning to use sodium azide for the first time as we wish to
store SEM samples for 3-4 months (rare samples available now for a
student project in November). I have found that very occasionally
something can grow in sodium cacodylate buffer. I think there are some
fungi that like it :)

Dave

-----Original Message-----
X-from: dianavd-at-eye.usyd.edu.au [mailto:dianavd-at-eye.usyd.edu.au]
Sent: 09 August 2005 02:11
To: David Patton

I've seen it as a constituent of a TEM fixative, so it shouldn't be a
problem. But why bother?

Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
}
}
} Email: elke.buschbeck-at-uc.edu
} Name: Elke
}
} Organization: University of Cincinnati
}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
}

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1. Is my face red! Only yesterday I read that with the new listserver
setup that hitting reply--intending to send mail to the person who
originated the initial email only--in fact sends the reply to the whole
listserver! Please disregard the email to Henderson. I'll try my best
not to make that mistake again! I'm sorry.

2. To the folks who sent me nasty, nearly obscene diatribes castigating
me for this simple mistake: please try and get a grip on life. Perhaps a
nice nap will help.

Ron Anderson
Microscopy Today

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6, 20 -- From randerson20-at-tampabay.rr.com Tue Aug 9 09:34:23 2005
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one can only wonder if these same people have time to
do anything else beside criticize others.
i have been on the receiving end of these diatribes.
and yes people do need to get a life.
john
ps and yes i meant to send this to the whole
listserver

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} 1. Is my face red! Only yesterday I read that with
} the new listserver
} setup that hitting reply--intending to send mail to
} the person who
} originated the initial email only--in fact sends the
} reply to the whole
} listserver! Please disregard the email to
} Henderson. I'll try my best
} not to make that mistake again! I'm sorry.
}
} 2. To the folks who sent me nasty, nearly obscene
} diatribes castigating
} me for this simple mistake: please try and get a
} grip on life. Perhaps a
} nice nap will help.
}
} Ron Anderson
} Microscopy Today
}
}
}
}
} ==============================Original
} Headers==============================
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} 09:34:23 2005
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} from MT!
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__________________________________________________
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i didn't know the listserver was offering home loans.

--- ypuizaluzec-at-microscopy.com wrote:

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} Hello
}
} Homeowner
}
} You have been pre-approved for a $402,974 Home Loan
} at a 4.53% Fixed Rate.
} This offer is being extended to you unconditionally
} and your credit is in no way a factor.
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} To take Advantage of this Limited Time opportunity
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} All we ask is that you visit our Website and
} complete
} The 1 minute post Approval Form
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} http://mrYC3sFjAhXM0.loanvoice.com/?name=rnnn
}
} Have a Good Day,
}
} Nelle Kendall
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} 8, 13 -- From ypuizaluzec-at-microscopy.com Tue Aug 9
} 08:07:44 2005
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On 8/9/05 12:24 AM, "ars-at-sem.com" {ars-at-sem.com} wrote:

}
}
}
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}
} Any idea if there will be a single hole per sample or multiple holes? Will
} you have any idea what the probable depth of the small hole will be (not
} including the thinning they are apparently doing to the substrate)? The
} hole's aspect ratio will have a lot to do with any analysis since a large
} ratio (small diameter hole, long bore) can make alignment with any imaging
} system a big problem.
}
} This is probably a good time to jump in and see if they can't adjust their
} experiment a little. Too often researchers jump before they can walk, and
} assume that an existing method can provide whatever information they need,
} without providing any compliance to existing techniques. A thinner
} substrate would require less preliminary FIB thinning on their part and if
} it were thin enough, could allow you to determine and measure the presence
} of a hole even if the bore weren't perfectly aligned with the beam.
}
} While they may be reluctant to discuss the work they are doing, details
} aren't really needed. It may be in their best interest to understand the
} hole formation process before extending it to such a thick substrate that
} requires multiple operations. Unfortunately, it may be up to you to point
} that out.
}
}
} Allen R. Sampson, Owner
} Advanced Research Systems
} 317 North 4th. Street
} Saint Charles, Illinois 60174
} phone (630) 513-7093 fax (630) 513-7092
} email mailto:ars-at-sem.com web www.sem.com
}
}
} On Monday, August 08, 2005 5:51 PM, jmkrupp-at-cats.ucsc.edu
} [SMTP:jmkrupp-at-cats.ucsc.edu] wrote:
} }
} }
} }
} }
} ------------------------------------------------------------------------
} ----
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} America
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} }
} } Hi:
} }
} } A guy came into the lab today asking me to image and measure some holes
} in
} } a silicon nitride wafer.
} }
} } OK, I say, how big?
} }
} } 2 nm he says, some maybe up to 70 nm.
} }
} } That's pretty small, I said. How did you plan on doing it?
} }
} } Don't know, that's why I came to you, says he.
} }
} } Here is what he's got. A chip about 1 mm thick with an FIB section out of
} } it that is like an inverted pyramid into the chip. The pyramid doesn't go
} } all the way through, but there is a thin area at the bottom that is
} } supposed to have the tiny hole. The chip is opaque in the TEM, way too
} } thick, the thin area is translucent at 80KV so we can sort of see where
} we
} } are going.
} }
} } We rigged up a special TEM holder so we could put the chip in the TEM,
} but
} } searching for the FIB hole, then the tiny hole inside that took a while.
} } Eventually I could see something that looked like a hole and it was about
} } 100 nm across. He said that was OK, it was a test hole and should be
} about
} } 100 nm.
} }
} } Finding this 100 nm hole was no picnic and I anticipate finding and
} } measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} } check the diameter and pop in another one.
} }
} } Anyone with some bright ideas about how to help this guy? We brainstormed
} } things like FESEM, AFM, some kind of diffraction with lasers, don't know
} if
} } any of them will work, don't have any more ideas.
} }
} } Thanks
} }
} } Jon
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
} }
} }
} }
} } ==============================Original
} Headers==============================
} } 15, 17 -- From jmkrupp-at-cats.ucsc.edu Mon Aug 8 17:48:38 2005
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} } 15, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
} } 15, 17 -- Subject: Looking for tiny holes
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} 7, 20 -- From ars-at-sem.com Tue Aug 9 00:20:36 2005
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}
} The thickness at a hole depends upon whether a precipitate fell out, which
leaves a thicker region around a hole. If a hole is formed by electropolishing
an annealled sample of a pure metal, or even some alloys, the area around the
hole may be very thin. 20 to 50 nanometers might be typical thicknesses
achieved.
Bernie Kestel
Argonne National Lab E-mail: kestel-at-anl.gov

==============================Original Headers==============================
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Sandy,
I saw that yesterday. I guess things are working out for Scott, if
his lab is growing. That's good.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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Colleagues;

I believe I have plugged the loop-hole that allowed the Housing
for Sale Email to get through the system earlier today. It was
a unique situation to say the least, but then again they are all
getting this way.

At the same time since I was editing the code I have also changed the
Listserver Software so that a reply should nolonger go automatically
to the Listserver but to the sender.

Nevertheless I caution you to please always look at what your sending.
It only takes a moment.

If you wish to send a copy of your "reply" to a message to the Listserver
you will now have to explicitly add the Listserver Email
address (microscopy-at-microscopy.com ) to your recepients list

Nestor
Your Friendly Neighborhood SysOp

--

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Sodium azide should be used very carefully. It has been used as a biological
preservative for a long time. In 1968 I was working in a CDC lab in Phoenix
(Yes, there was a CDC branch there at that time) where we used sodium azide to
preserve hepatitis antigens. One of the technicians was using an aspiration
tube attached to a pipette to transfer sodium azide solution to an antigen
preparation. She coughed and sucked the solution into her lungs. She was on
the floor gasping for breath within 15 seconds. There was an MD sitting in
his office next to the technician's lab. He heard her fall to the floor. He
new what she was doing and determined what had happened. He had what he
called a "universal antitdote" in his office. He administered the antidote
and gave her mouth-to-mouth resuscitation and she survived.

I know (I hope!!) people don't use aspiration tubes today but this incident
sure brought home to me how how quickly sodium azide works. You may want to
look at the following CDC URL:
http://www.bt.cdc.gov/agent/sodiumazide/basics/facts.asp Although
this article states that there is no specific antidote for sodium azide
poisoning, in the incident I related above, an antidote was given. I don't
remember what it was.

} ----------
} From: dianavd-at-eye.usyd.edu.au
} Reply To: microscopy-at-microscopy.com
} Sent: Monday, August 8, 2005 6:11 PM
} To: John Wheatley
} Subject: [Microscopy] viaWWW: Sodium Azide and TEM
}
}
}
}
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} I've seen it as a constituent of a TEM fixative, so it shouldn't be a
} problem. But why bother?
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
} }
} }
} } Email: elke.buschbeck-at-uc.edu
} } Name: Elke
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
} }
} } Question: Does anyone know if it is o.k. to use sodium azide with TEM
} } preps? Does it wash out o.k. or will it leave a nasty deposit?
} } Thanks
} }
}
}
} ==============================Original Headers==============================
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Sodium azide should be used very carefully. It has been used as a
biological preservative for a long time. In 1968 I was working in a
CDC lab in Phoenix (Yes, there was a CDC branch there at that time)
where we used sodium azide to preserve hepatitis antigens. One of
the technicians was using an aspiration tube attached to a pipette to
transfer sodium azide solution to an antigen preparation. She
coughed and sucked the solution into her lungs. She was on the floor
gasping for breath within 15 seconds. There was an MD sitting in
his office next to the technician's lab. He heard her fall to the
floor. He new what she was doing and determined what had happened.
He had what he called a "universal antitdote" in his office. He
administered the antidote and gave her mouth-to-mouth resuscitation
and she survived.

I know (I hope!!) people don't use aspiration tubes today but this
incident sure brought home to me how how quickly sodium azide works.
You may want to look at the following CDC URL:
http://www.bt.cdc.gov/agent/sodiumazide/basics/facts.asp
Although this article states that there is no specific antidote for
sodium azide poisoning, in the incident I related above, an antidote
was given. I don't remember what it was.

} ----------
} From: dianavd-at-eye.usyd.edu.au
} Reply To: microscopy-at-microscopy.com
} Sent: Monday, August 8, 2005 6:11 PM
} To: John Wheatley
} Subject: [Microscopy] viaWWW: Sodium Azide and TEM
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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}
} I've seen it as a constituent of a TEM fixative, so it shouldn't be a
} problem. But why bother?
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
} }
} }
} } Email: elke.buschbeck-at-uc.edu
} } Name: Elke
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
} }
} } Question: Does anyone know if it is o.k. to use sodium azide with TEM
} } preps? Does it wash out o.k. or will it leave a nasty deposit?
} } Thanks
} }
}
}
} ==============================Original
} Headers==============================
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} (machaon.med.usyd.edu.au [129.78.36.30])
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7, 26 -- From JOHN.WHEATLEY-at-asu.edu Tue Aug 9 12:32:58 2005
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We use a standard SEM to work with nanometer-scale features (~50 nm) in
silicon
nitride. You might consider using electron diffraction. If using a 10
keV beam,
(wavelength of .12 angstroms), a circular aperture with a diameter of 2
nm would
create a circular diffraction pattern (described by an Airy disk). With these
parameters, the radius of the first zero is given by R = 0.007*(distance from
the aperture).

Dunno much about TEM and standard detectors used, but if you have an electron
detector with a spatial resolution of 10 microns placed 1 cm below the sample,
you would be able to resolve the central diffraction order (thereby measuring
the hole diameter). To do this, you'd turn off the scanning mechanism for the
microscope, and you'd want to focus quite a bit beyond the hole itself (to
ensure the electron wavefronts extend across the diameter of the hole.

Anyway, I'm sure there are more straightforward solutions, but this is just an
idea. We do similar stuff all the time.

--
Ben McMorran
Research Assistant, Atom Optics Group
Department of Physics
University of Arizona
1118 E 4th St
Tucson, AZ
USA

ph. 520-621-2688

Quoting jmkrupp-at-cats.ucsc.edu:

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} ----------------------------------------------------------------------------
}
} Hi:
}
} A guy came into the lab today asking me to image and measure some holes in
} a silicon nitride wafer.
}
} OK, I say, how big?
}
} 2 nm he says, some maybe up to 70 nm.
}
} That's pretty small, I said. How did you plan on doing it?
}
} Don't know, that's why I came to you, says he.
}
} Here is what he's got. A chip about 1 mm thick with an FIB section out of
} it that is like an inverted pyramid into the chip. The pyramid doesn't go
} all the way through, but there is a thin area at the bottom that is
} supposed to have the tiny hole. The chip is opaque in the TEM, way too
} thick, the thin area is translucent at 80KV so we can sort of see where we
} are going.
}
} We rigged up a special TEM holder so we could put the chip in the TEM, but
} searching for the FIB hole, then the tiny hole inside that took a while.
} Eventually I could see something that looked like a hole and it was about
} 100 nm across. He said that was OK, it was a test hole and should be about
} 100 nm.
}
} Finding this 100 nm hole was no picnic and I anticipate finding and
} measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} check the diameter and pop in another one.
}
} Anyone with some bright ideas about how to help this guy? We brainstormed
} things like FESEM, AFM, some kind of diffraction with lasers, don't know if
} any of them will work, don't have any more ideas.
}
} Thanks
}
} Jon
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
} ==============================Original Headers==============================
} 15, 17 -- From jmkrupp-at-cats.ucsc.edu Mon Aug 8 17:48:38 2005
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} 15, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
} 15, 17 -- Subject: Looking for tiny holes
} 15, 17 -- X-UCSC-CATS-MailScanner: Found to be clean
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9, 27 -- From mcmorran-at-physics.arizona.edu Tue Aug 9 14:30:30 2005
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I didn't see that you got a response on this, so I thought that I would
do so.

I built a FIM/IAP for my dissertation work back in the early to mid
80's. Mine was made using UHV stainless steel components. I was
familiar with most of the people in the field at that time and didn't
know anyone that was still putting together glass systems. I think that
it has been a long time since people have been using glass systems for
FIM. When they were, mostly they had their systems custom made by their
in-house glass blowers.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: tttan-at-simtech.a-star.edu.sg [mailto:tttan-at-simtech.a-star.edu.sg]
Sent: Tuesday, August 02, 2005 11:05 AM
To: Walck-at-SouthBayTech.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tttan-at-simtech.a-star.edu.sg) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
August 1, 2005 at 20:24:16
------------------------------------------------------------------------
---

Email: tttan-at-simtech.a-star.edu.sg
Name: TT Tan

Organization: Singapore Inst. of Manuf. Tech.

Title-Subject: [Filtered] Field Ion Microscope

Question: Hi all,

I would like to know from who can I buy the glassware to do field ion
microscopy.

I am looking for a quartz ware that can do the job. Preferably one
that allows me to fit onto a NW40 joint.

Thanks in advance.

------------------------------------------------------------------------
---

==============================Original
Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Tue Aug 2 12:59:37 2005 9, 12 --
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21, 26 -- From walck-at-southbaytech.com Tue Aug 9 22:25:48 2005
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21, 26 -- From: "Scott Walck" {walck-at-southbaytech.com}
21, 26 -- To: {microscopy-at-microscopy.com}
21, 26 -- Subject: RE: [Microscopy] viaWWW: Field Ion Microscope
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (montiorm-at-ucmail.uc.edu) from http://microscopy.com/MLFormMail.html on Thursday, July 28, 2005 at 12:57:07
---------------------------------------------------------------------------

Email: montiorm-at-ucmail.uc.edu
Name: Richard Montione

Organization: University of Cincinnati

Title-Subject: [Filtered] TCS 4D available

Question: RE: TCS 4D

To anyone interested,

I have a Leica TCS 4D confocal system originally installed in 1994. It has a Kr/Ar laser and a water cooled UV laser, with a Leitz DMRBE upright microscope and a number of lenses. It has the usual problems associated with age but was still functional when it was disassembled for storage.

If anyone is interested and would like more information please contact me at montiorm-at-uc.edu

Richard Montione

Electron Microscopy Facility

Department of Pathology

University of Cincinnati

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==============================Original Headers==============================
20, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:26:33 2005
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20, 12 -- From: montiorm-at-ucmail.uc.edu (by way of MicroscopyListserver)
20, 12 -- Subject: viaWWW: TCS 4D available
20, 12 -- Content-Type: text/plain; charset="us-ascii"
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (msimms-at-tracelabs.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, July 29, 2005 at 10:06:10
---------------------------------------------------------------------------

Email: msimms-at-tracelabs.com
Name: Michael Simms

Organization: Trace Laboratories - Central

Title-Subject: [Filtered] Measurement of ink depth/contrast

Question: Hello Gentlemen,
I work for an independent testing organization, Trace Laboratories - Central.
We have been asked to have printing ink measured for depth and contrast. Laser printing is done on the insulation sleeve of a cable.
per Sikorsky specification SS7333, Paragraph 4.6.5
This asks for measuring to an accuracy of 0.0001 inch with a contour projector or calibrated microscope or Zygo measuring device. The contrast measurement is suggested to be made with a Spectrum Technology CMS^2 Contrast Measuring System. I would need evidence of either an accredited laboratory or calibration to pass on to the customer.

Is this something that someone associated with this resource might be able to quote?

Regards,
Mike

Mike Simms
Chemist
Trace Laboratories - Central
1150 W. Euclid Ave.
Palatine, IL 60067

phone 847-934-5300
fax 847-934-4600
www.tracelabs.com

---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:29:45 2005
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9, 12 -- From: msimms-at-tracelabs.com (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: Measurement of ink depth/contrast
9, 12 -- Content-Type: text/plain; charset="us-ascii"
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (stefan.diller-at-t-online.de) from http://microscopy.com/MLFormMail.html on Saturday, July 30, 2005 at 13:43:48
---------------------------------------------------------------------------

Email: stefan.diller-at-t-online.de
Name: Stefan Diller

Title-Subject: [Filtered] Reichert Histostat 8035 Paraffin Embedder manual needed

Question: Dear members,
I am looking urgently for a user manual and if possible a service manual or the electronic layout for the Reichert Histostat Paraffin Embedder Modell 8035.

Best regards,
Stefan Diller

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:31:03 2005
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6, 12 -- From: stefan.diller-at-t-online.de (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Reichert Histostat 8035 Paraffin Embedder manual needed
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ken.blight-at-cancer.org.uk) from http://microscopy.com/MLFormMail.html on Wednesday, August 3, 2005 at 10:40:02
---------------------------------------------------------------------------

Email: ken.blight-at-cancer.org.uk
Name: Ken Blight

Organization: cancer research uk

Title-Subject: [Filtered] Drosophila eye ultrathin cryosections

Question: I have processed Drosophila eyes for immunolabelling (EM) using the Tokuyasu technique and the results are, to say the least, very disappointing. The eyes floated in the fixative which didn't help matters. The problem looks to be spacial when I compare the results to standard resin TEM preparation. Maybe there is some problem with buffers, fixation or section expansion. Does anyone have experience of these tough little beasties?

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==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:32:28 2005
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: ken.blight-at-cancer.org.uk (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Drosophila eye ultrathin cryosections
6, 12 -- Content-Type: text/plain; charset="us-ascii"
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 3, 2005 at 18:07:30
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver: Re: Cambridge S250

Question: Hi,

I'm not too familiar with these despite the fact I have two of them gathering dust. But I have heard them disfavorably compared to the S200. Mine may be site-rigged for handling semi wafers (and there's a chance both came from the same facility, at different times... a hunch) but in mine the stage uses little more substantial than pvc tubing to transmit the z-axis knob to the stage: horrible hysteresis.

I suspect these and related scopes were enormously popular, and I know for a fact this 'scope has die-hard fans.

LaB6 is typical but it wouldn't surprise me if there was an active aftermarket upgrade to FE: indeed one of mine may have one. On the other hand, you may have missed the bulk of the aftermarket boat by about 5 years: lots of parts for old scopes falling off of price lists.... but sometimes onto "clearance" lists :)

But I'd say even if your own scope leaves a lot to be desired, trading it for another old scope like this? I'd say deosn't quite make sense unless you have a special requirement. Ultimately, it just may not be worth the shipping: which of course is dicey unless there's at least a few hours teardown and re-assembly. Better the devil you know.

I hope more people respond, I've been back and forth as to whether to try to get one or both of mine working for about 6 years now :)

-Jeff

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==============================Original Headers==============================
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12, 12 -- To: microscopy-at-microscopy.com
12, 12 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
12, 12 -- Subject: viaWWW: Cambridge S250
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 3, 2005 at 18:41:52
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver:b Re: Osmium Vapors

Question: I don't have experience with osmium compounds, and my suggestion may not be very practical (read: expensive) unless you have piles of vacuum fittings lying around like I do, but on occasion when wanting to "absolutely" seal something up I've used a KF50 stainless nipple (or bellows, sometimes)of appropriate length, a pair of stainless blanks, clamps, o-rings etc. so that the item could be sealed "vacuum tight". Of course it's all relative but I wouldn't think much vapour will diffuse though the ~5mm of highly compressed Viton. The clamps are a bit clumsy to put on but very quick to come off: I'm not sure you can get a quick-release clamp in KF50 but that would make it almost one-handed. You could probably save money and thru-gassing by having a single-ended arrangment, ie: a pipe section with a KF50 flange welded on one end and a cap welded on the other. Some (if not most) of the hi-vac houses weld their standard catalog items on-site, and some don't charge much extra (~%20 premium) for custom items from stock parts.

I think the idea of absorbants/reactants holds a lot of potential. Activated charcoal comes immediately to mind.
Is it so reactive as to present a fire hazard if quickly adsorbed onto the huge surface area?

-Jeff

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==============================Original Headers==============================
9, 14 -- From zaluzec-at-microscopy.com Tue Aug 9 22:34:38 2005
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9, 14 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
9, 14 -- Subject: viaWWW: Re: Osmium Vapors
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (omelon-at-geology.utoronto.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, August 4, 2005 at 16:57:53
---------------------------------------------------------------------------

Email: omelon-at-geology.utoronto.ca
Name: Christopher R. Omelon

Organization: University of Toronto

Title-Subject: [Filtered] further hardening of LR White

Question: I have tried embedding large samples (~ 1cm3) of porous rock material in a variety of resins and find that LR White (Hard) provides the best infilling due to its low viscosity. That being said, the blocks are not as hard as when using epoxide resins such as EMBED 812. I am cold-curing (i.e. with accelerator) at both room temperature and at 4?C with mixed results, but even in the best case the blocks are not as hard as I would expect. My question: can I further harden the blocks by now placing these samples in the oven at 60?C? My reasoning is that resin within the block (i.e. beneath the surface and therefore not exposed to oxygen) will proceed with polymerization at a faster rate than if I left the blocks at room temperature. Or is the reaction now at completion? Thanks

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==============================Original Headers==============================
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005 at 12:35:33
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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: We have bags of Kodak Dektol. Does anyone know if I can use this to develop Kodak Em film 4489? At what dilution, for how long?
Thanks for any help.

Stacey Andringa

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Hi All,

We have just had an ergonomist look at our Hitachi S4100 set up as the
primary user is beginning to have some problems which could get very
nasty as time goes on.

Has anyone out there had similar issues? And if so, what issues were
felt to be critical and what solutions were offered?

One of the biggest issues (according to the ergonomist) is the position
of the screens. They are CRT's embedded in a console and as such can't
be moved. Has anyone tried to take the video signals from one of these
systems (or similar) and put them onto a LCD monitor? If so, how was it
done and how successful was it? (these screens are also beginning to
lose their intensity which isn't helping!)

Another issue is the arrangement of the console and column. The
ergonomist feels that they would be better at 90 degrees to each other
but the cable length between the column and console prevents that. Has
anyone tried to lengthen these cables (some go to the HV tank)?

Any help or information would be appreciated.

Cheers

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.

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Since I'm the Manager for the Educational Division I have to put my 2 cents
in.....

NEVER buy a microscope just because it is affordable. I always suggest
getting in a local dealer who can
provide you with a demonstration AND be there after the sale in case you
need service.

There are many "off brand" microscopes out there that are a good way to
through away money. I have been
in many labs where whole sets of microscopes are on the shell because one
or two starting smoking and the
lab manager was afraid to use them. I have also tried servicing some of
these off brands and the materials
used and the machining makes them good ship achors :-)

For your own best interest, call a local dealer and get a demonstration -
it's also good to ask for references from
people who have used the microscope you are looking to buy. Remember the
saying "you get what you pay for".
Bad quality microscopes will prevent you from being able show the students
what you are trying to teach.

If you have any questions, please do not hesitate to contact me.

Gretchen

Gretchen Ziegler, Sales Manager, Educational Division
Leica Microsystems, Inc.
P.O. Box 151 - Ocean Grove, NJ 07756
Phone: 732-897-9506 - Fax: 847-236-3013
Voicemail: 1-800-248-0665 ext. 5131

ballardmark-at-gmail
.com To: Gretchen.Ziegler-at-leica-microsystems.com
cc:
07/28/2005 09:08 Subject: [Microscopy] AskAMicroscopist: an affordable student microscope
PM
Please respond to
microscopy

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Email: ballardmark-at-gmail.com
Name: Marcello

Organization: None

Education: 9-12th Grade High School

Location: Orlando, Florida

Question: Hi to all,

i am trying to buy the most afforable microscopy, but that will be
able to help me with biology and chemistry.

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Hello Colin,

We have an Hitachi S-4000 which I believe is very similar to the S-4100. We
have encountered both of this issues and have addressed them as follows:

We attempted to turn the column cabinet in order to enable users better
access to the manual stage controls. As you observed the cable length is
insufficient to accomplish more than 20 -30 degree of rotation. We also
determined that turning the column introduced a new problem...it became
difficult to introduce a sample into the SEC port (which is our primary
sample entry port). Our solution was to purchase a motorized stage. While
this was a costly upgrade, it was money well spent. The 5 axis stage we
purchased from a company called E.Fjeld(http://www.efjeld.com/P_5500.htm)has
performed much better that anticipated. As expected wear and tear on the
user is dramatically improved! In addition the stage motion is much more
precise and backlash is nonexistent.

On the S-4000 there was a simple solution to adding a high resolution CRT
type monitor. A BNC port accessing the display crt already existed. If you
open the back lower console panel you should see two BNC ports in the upper
middle region (ours are unlabelled). We connected a cable from the left
port to the B & W monitor. If you have schematics for the system you should
be able to determine if this port exist on your system. Unfortunately I
don't know much about LCD monitors and their cabling options so this could
be an issue when selecting a monitor. However, we have been satisfied with
the B & W CRT. Also, we haven't noticed any adverse field effects while the
CRT monitor is in use.

While not addressed in your post a third area relating to ergonomics was
addressed by elimination of the Polaroid camera system. We purchased a
passive acquisition system from PCI
Quartz(http://www.qrtz.com/acquisition.html) which allows us to capture
digital images.

While the upgrades were costly they have improved the user interface,
increased productivity, and added to the useful lifetime of the instrument.
The system is 16+ years old and continues to produce quality results in a
digital environment. I'm not sure how much, if any, of this information is
helpful or relevant? However, if you have any question please feel free to
contact me directly. Good luck, jr.

Disclaimer: These are my opinions only and do not reflect those of my
employer. While I am a satisfied customer I have no financial ties to any of
these manufacturers. All of the upgrades mentioned are available from
multiple vendors and it is suggest that you investigate all options prior to
making any purchases for your system.

John A. Robson
Boehringer Ingelheim Pharmaceuticals, Inc.
PO Box 368
900 Ridgebury Rd
Ridgefield, CT 06877

Phone (203)798-5640
Fax (203)798-5698
e-mail jrobson-at-RDG.boehringer-ingelheim.com

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That's an easy one.....no! Dektol is for developing photo paper, not
film. Take it from someone who did this by mistake once and got
negatives with grain the size of gravel and films so thick you could use
them to view eclipses. It just might be possible with enough testing
and fooling to get negatives you could actually see through, but the
quality would be pretty awful anyway.

If you don't make prints anymore, donate your Dektol to your local
photography classes.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

-----Original Message-----
X-from: Stacey.Andringa-at-uc.edu [mailto:Stacey.Andringa-at-uc.edu]
Sent: Tuesday, August 09, 2005 10:40 PM
To: Tindall, Randy D.

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Stacey.Andringa-at-uc.edu) from
http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005 at
12:35:33
------------------------------------------------------------------------
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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: We have bags of Kodak Dektol. Does anyone know if I can use
this to develop Kodak Em film 4489? At what dilution, for how long?
Thanks for any help.

Stacey Andringa

------------------------------------------------------------------------
---

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Hello All,
Can anyone advise this new user to our lab? His prep/questions are
below. I think that he needs negative staining. How best to prep this
sample?/fixation/washing out the sucrose?? and what type of filmed
grids are being used these days??Formvar/Pioloform??other??

-----------------------

Basically what I have is a 100nm virus particle bound to a 100nm
liposome molecule by a Nickel NTA "bridge". In short, I would like a
picture of this complex. So, If I had this complex (usually in
~10-20%sucrose solution), what would I do from there as far a
preparation?

----------------------
Thanks,
Linda

Linda M. Fox
Loyola University
Stritch School of Medicine
Core Imaging Facility
2160 S. First Ave.
Maywood, Il 60153
Bld. 102 Room 0617
1-708-216-3395
lfox1-at-lumc.edu

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Bill,

Ron has been trying to get me to rejoin MT, so ...
This would make an excellent article, would you be willing to write
it up for MT?
Note, we'd also like articles on cryoTEM and tomography.

Phil

} On Aug 5, 2005, at 5:36 PM, sdmegs09-at-yahoo.com wrote:
}
} } Question: I am trying to fix plant material for EDS in the TEM and I
} } cannot find literature stating the fixation techniques. Is fixation
} } different for EDS?
} }
} Dear Meghan,
} The preparation methods for any sample on which you are doing EDS or
} other analytic techniques must take into account the elements you are
} trying to analyze, so, for example, if you are looking for
} water-soluble elements like Na or Cl ion, do not rinse the tissue in
} solvents that will remove them. Note, however, that if you are looking
} for Cl bound in organic compounds, such as polychlorinated biphenyls,
} washing out the ion will enable you to distinguish the organochlorine.
} For accurate quantitation, it is a good idea, if possible, to examine
} the material untreated, except for freezing and perhaps sectioning,
} then dehydrate by lyophylization in the scope and redo the analysis.
} This will give accurate values for volatile elements and you can
} improve the quantitation by taking the wet/dry ratios from elements
} that are not affected by the drying process. Another consideration,
} for the case that the elements of interest are not affected by your
} preparation process, is not to use a stain that interferes with lines
} from those elements. Pb and S are notorious for this, and one case I
} ran into was trying to analyze Pt and Ir in a specimen that was treated
} with Os and given to me. Since your specimens may not be suitable for
} observation in the TEM when not prepared in some way, and since you may
} not have access to cryopreparation methods, you need to keep in mind
} the general principles that, whatever the preparation steps, they
} cannot affect the concentration of the elements of interest, and cannot
} interfere with the lines you want to measure. Good luck.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

==============================Original Headers==============================
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Dear Listers,

Has anyone used a low melting point (LMP) agarose to
encapsulate cells for TEM? If yes, which resin is
best suited for LMP agarose encapsulted cells? We
have in the lab Durcupan ACM resin, EMbed-812, LRW
resin kits.

The samples were encapsulated after buffer wash, after
osmium fixation.

Thank you,

Claire

__________________________________________________
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==============================Original Headers==============================
6, 18 -- From lukeclaire-at-yahoo.com Wed Aug 10 10:25:08 2005
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6, 18 -- From: claire haueter {lukeclaire-at-yahoo.com}
6, 18 -- Subject: TEM of agar encapsulated sample
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We use Agarose type IX (Sigma). It works well with Spurrs and Araldite
(from TAAB in the UK). This summer I have had a problem infiltrating
cells in agarose with LR White (polymerising at 55 degrees C) despite
success in two previous years. This is probably a problem with me
rather than the products.

Dave

-----Original Message-----
X-from: lukeclaire-at-yahoo.com [mailto:lukeclaire-at-yahoo.com]
Sent: 10 August 2005 16:26
To: David Patton

Dear Listers,

Has anyone used a low melting point (LMP) agarose to
encapsulate cells for TEM? If yes, which resin is
best suited for LMP agarose encapsulted cells? We
have in the lab Durcupan ACM resin, EMbed-812, LRW
resin kits.

The samples were encapsulated after buffer wash, after
osmium fixation.

Thank you,

Claire

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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6, 18 -- From: claire haueter {lukeclaire-at-yahoo.com}
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18, 30 -- From David.Patton-at-uwe.ac.uk Wed Aug 10 10:45:23 2005
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I have used LMP many times for TEM. I process it just like I would a
block of tissue and go into EMbed-812 or Spurs.
Good luck
David

On Aug 10, 2005, at 8:29 AM, lukeclaire-at-yahoo.com wrote:

}
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}
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} Dear Listers,
}
} Has anyone used a low melting point (LMP) agarose to
} encapsulate cells for TEM? If yes, which resin is
} best suited for LMP agarose encapsulted cells? We
} have in the lab Durcupan ACM resin, EMbed-812, LRW
} resin kits.
}
} The samples were encapsulated after buffer wash, after
} osmium fixation.
}
} Thank you,
}
} Claire
}
} __________________________________________________
} Do You Yahoo!?
} Tired of spam? Yahoo! Mail has the best spam protection around
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} Headers==============================
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} 6, 18 -- From: claire haueter {lukeclaire-at-yahoo.com}
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5, 22 -- From Elliott-at-arizona.edu Wed Aug 10 10:53:33 2005
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Hello all,

We have a group of labs that are
attempting to standardize an Osmium-
ZnI2 staining procedure for detection of
chloride cells in trout gills.

The protocol seems foolproof: immerse
freshly dissected gill pieces in a freshly-
made solution of 1 part 2% OsO4 to 4
parts 3% ZnI2 for 24 at 4�C, dehydrate,
clear in Histochoice, paraffin-embed,
section, de- paraffinize and mount in
Cytoseal60-xyl.
There seems to be no consistency in
results. In fact, more often or not we see
no staining of the chloride cells, though
the gill filaments themselves display the
rich tan colour of well-osmicated tissue.

Does anyone have experience with this
technique? What can cause it to fail? Is
age/source of reagents an important
factor? Are there any tricks to making
the procedure reliable?

==============================Original Headers==============================
5, 21 -- From AOCHALSK-at-science.uottawa.ca Wed Aug 10 11:14:17 2005
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5, 21 -- Organization: Univ. of/d' Ottawa
5, 21 -- To: Microscopy-at-microscopy.com
5, 21 -- Date: Wed, 10 Aug 2005 12:13:55 -0400
5, 21 -- Subject: LM zinc-osmium staining of trout chloride cells:woes
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On Aug 10, 2005, at 6:30 AM, lfox1-at-lumc.edu wrote:

} Can anyone advise this new user to our lab? His prep/questions are
} below. I think that he needs negative staining. How best to prep this
} sample?/fixation/washing out the sucrose?? and what type of filmed
} grids are being used these days??Formvar/Pioloform??other??
}
} -----------------------
}
} Basically what I have is a 100nm virus particle bound to a 100nm
} liposome molecule by a Nickel NTA "bridge". In short, I would like a
} picture of this complex. So, If I had this complex (usually in
} ~10-20%sucrose solution), what would I do from there as far a
} preparation?
}
Dear Linda,
If the specimen would not be perturbed by removal of the sucrose,
either of the techniques of negative staining or cryoEM would be
improved, and for negative staining with UAc, the buffer should not
contain phosphate. It would be best if the specimen could be in a
dilute buffer, either tris or a Good buffer would be suitable. I would
use carbon-formvar coated grids for negative staining and either lacy
carbon or, better, Quantifoils for cryo imaging. Depending on the
resolution desired, the tolerance of the specimen for low pH, the
equipment available, etc., the easiest procedure (and the one that I
would start with) is negative staining with UAc, which is at pH = ~3,
or phosphotungstate or phosphomolybdate, which are at pH = ~7. After
getting the specimen into the appropriate buffer, put 5 ul of the
appropriately diluted material onto a glow-discharged carbon-formvar
grid. Add 5 ul of a 2% solution of the staining compound, and let
stand for 1 min. Blot most of the liquid off with a bit of #1 filter
paper applied to the edge of the grid, add 10 ul of a 1% solution of
stain, let stand for 1 min, then thoroughly blot off the liquid. After
seeing whether the dilution of the specimen gives a good amount of
material on the grid--enough to see several particles in each (film or
CCD) frame--adjust the concentration if necessary. If cryoEM will be
useful, prepare the specimen at a concentration of about twice that
used for negative staining, and plunge-freeze it.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu

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Claire,
Note that low melting point agarose (and probably all types)
is nicely stained by fast green. We would make a few percent solution
in 100% ethanol and add a drop to our agarose blobs. Otherwise, they
can vanish in the solutions and lead to some frustrations. I suppose
fast green would also dissolve in acetone but I don't know that for a
fact. However, now we trap small samples between formvar films on
wire loops and like that much better than agarose (except on the days
when the formvar won't cast, 8-().

HTH,
Tobias
}
}
} We use Agarose type IX (Sigma). It works well with Spurrs and Araldite
} (from TAAB in the UK). This summer I have had a problem infiltrating
} cells in agarose with LR White (polymerising at 55 degrees C) despite
} success in two previous years. This is probably a problem with me
} rather than the products.
}
} Dave
}
} -----Original Message-----
} X-from: lukeclaire-at-yahoo.com [mailto:lukeclaire-at-yahoo.com]
} Sent: 10 August 2005 16:26
} To: David Patton
} Subject: [Microscopy] TEM of agar encapsulated sample
}
}
} ----------------
} ----
}
} Dear Listers,
}
} Has anyone used a low melting point (LMP) agarose to
} encapsulate cells for TEM? If yes, which resin is
} best suited for LMP agarose encapsulted cells? We
} have in the lab Durcupan ACM resin, EMbed-812, LRW
} resin kits.
}
} The samples were encapsulated after buffer wash, after
} osmium fixation.
}
} Thank you,
}
} Claire
}
} __________________________________________________
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} 18, 30 -- From David.Patton-at-uwe.ac.uk Wed Aug 10 10:45:23 2005
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--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
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This is not entirely true. Many years ago, I saw an article suggesting the
use of Dektol for developing 4489 and Electron Image Plates (glass). The
article demonstrated a more linear curve for exposure using 80-100kV.

I used Dektol for years after reading this article with great results. I
used this formula with SO-163: 5500 ML water + 400 ML Full strength Dektol.
I would basically make up the regular Dektol solution of photographic paper
development and from this solution would pour off 400 MLs. Consistent
results for printing with No. 3 grade paper were common.

You may have to experiment with scope intensity to exposure. I was able to
set-up my scope exposure was 2 seconds with a development of the regular 4
minutes exposure with nitrogen burst for 2 seconds every 10 seconds.

As for grain....what grain? Is a 3 x 4 foot enlargement ok for grain?

Ken
_______________________________________
Kenneth L. Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N Willamette Blvd.
Portland, OR 97203 USA

Tel.: 503.943.8861
Email: tiekotte-at-up.edu

On 8/10/05 6:30 AM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} That's an easy one.....no! Dektol is for developing photo paper, not
} film. Take it from someone who did this by mistake once and got
} negatives with grain the size of gravel and films so thick you could use
} them to view eclipses. It just might be possible with enough testing
} and fooling to get negatives you could actually see through, but the
} quality would be pretty awful anyway.
}
} If you don't make prints anymore, donate your Dektol to your local
} photography classes.
}
} Good luck,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
} -----Original Message-----
} X-from: Stacey.Andringa-at-uc.edu [mailto:Stacey.Andringa-at-uc.edu]
} Sent: Tuesday, August 09, 2005 10:40 PM
} To: Tindall, Randy D.
} Subject: [Microscopy] viaWWW: Kodak Dektol
}
}
}
}
} ------------------------------------------------------------------------
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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (Stacey.Andringa-at-uc.edu) from
} http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005 at
} 12:35:33
} ------------------------------------------------------------------------
} ---
}
} Email: Stacey.Andringa-at-uc.edu
} Name: Stacey Andringa
}
} Organization: University of Cincinnati
}
} Title-Subject: [Filtered] MListserver:
}
} Question: We have bags of Kodak Dektol. Does anyone know if I can use
} this to develop Kodak Em film 4489? At what dilution, for how long?
} Thanks for any help.
}
} Stacey Andringa
}
} ------------------------------------------------------------------------
} ---
}
} ==============================Original
} Headers==============================
} 7, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:37:06 2005 7, 12 --
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} 19, 24 -- From TindallR-at-missouri.edu Wed Aug 10 08:30:28 2005
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} 19, 24 -- Date: Wed, 10 Aug 2005 08:30:26 -0500
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} 19, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
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==============================Original Headers==============================
10, 19 -- From tiekotte-at-up.edu Wed Aug 10 13:49:08 2005
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10, 19 -- User-Agent: Microsoft-Entourage/11.0.0.040405
10, 19 -- Date: Wed, 10 Aug 2005 11:49:34 -0700
10, 19 -- Subject: Re: [Microscopy] RE: viaWWW: Kodak Dektol
10, 19 -- From: Ken Tiekotter {tiekotte-at-up.edu}
10, 19 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} ,
10, 19 -- Stacey Andringa {Stacey.Andringa-at-uc.edu}
10, 19 -- CC: "Tindall, Randy D." {TindallR-at-missouri.edu}
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Wow....I stand corrected. In 40 years of darkroom work, this is the
first I have heard of using Dektol successfully for film development,
except for a few avante-garde photographers using it for special effects
(i.e., grain). My life has been more sheltered than I thought, I guess.

I take it you have never used it with 4489? Just curious.

Randy

-----Original Message-----
X-from: Ken Tiekotter [mailto:tiekotte-at-up.edu]
Sent: Wednesday, August 10, 2005 1:50 PM
To: microscopy-at-microscopy.com; Stacey Andringa
Cc: Tindall, Randy D.

Randy,
I did use it with 4489, but liked the linearity of SO-163 compared with
Kodak plate film and so switched to SO-163. I no longer use film or the
darkroom as I have gone 100% digital.

Ken

On 8/10/05 11:58 AM, "Tindall, Randy D." {TindallR-at-missouri.edu} wrote:

} Wow....I stand corrected. In 40 years of darkroom work, this is the
} first I have heard of using Dektol successfully for film development,
} except for a few avante-garde photographers using it for special effects
} (i.e., grain). My life has been more sheltered than I thought, I guess.
}
} I take it you have never used it with 4489? Just curious.
}
} Randy
}
}
}
} -----Original Message-----
} From: Ken Tiekotter [mailto:tiekotte-at-up.edu]
} Sent: Wednesday, August 10, 2005 1:50 PM
} To: microscopy-at-microscopy.com; Stacey Andringa
} Cc: Tindall, Randy D.
} Subject: Re: [Microscopy] RE: viaWWW: Kodak Dektol
}
} This is not entirely true. Many years ago, I saw an article suggesting
} the use of Dektol for developing 4489 and Electron Image Plates (glass).
} The article demonstrated a more linear curve for exposure using
} 80-100kV.
}
} I used Dektol for years after reading this article with great results.
} I used this formula with SO-163: 5500 ML water + 400 ML Full strength
} Dektol.
} I would basically make up the regular Dektol solution of photographic
} paper development and from this solution would pour off 400 MLs.
} Consistent results for printing with No. 3 grade paper were common.
}
} You may have to experiment with scope intensity to exposure. I was able
} to set-up my scope exposure was 2 seconds with a development of the
} regular 4 minutes exposure with nitrogen burst for 2 seconds every 10
} seconds.
}
} As for grain....what grain? Is a 3 x 4 foot enlargement ok for grain?
}
} Ken
} _______________________________________
} Kenneth L. Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N Willamette Blvd.
} Portland, OR 97203 USA
}
} Tel.: 503.943.8861
} Email: tiekotte-at-up.edu
}
}
} On 8/10/05 6:30 AM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
} wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------
} } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
}
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ------
} }
} } That's an easy one.....no! Dektol is for developing photo paper, not
}
} } film. Take it from someone who did this by mistake once and got
} } negatives with grain the size of gravel and films so thick you could
} } use them to view eclipses. It just might be possible with enough
} } testing and fooling to get negatives you could actually see through,
} } but the quality would be pretty awful anyway.
} }
} } If you don't make prints anymore, donate your Dektol to your local
} } photography classes.
} }
} } Good luck,
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} }
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: Stacey.Andringa-at-uc.edu [mailto:Stacey.Andringa-at-uc.edu]
} } Sent: Tuesday, August 09, 2005 10:40 PM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] viaWWW: Kodak Dektol
} }
} }
} }
} }
} } ----------------------------------------------------------------------
} } --
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } --
} } ----
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (Stacey.Andringa-at-uc.edu) from
} } http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005
} } at
} } 12:35:33
} } ----------------------------------------------------------------------
} } --
} } ---
} }
} } Email: Stacey.Andringa-at-uc.edu
} } Name: Stacey Andringa
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:
} }
} } Question: We have bags of Kodak Dektol. Does anyone know if I can use
} } this to develop Kodak Em film 4489? At what dilution, for how long?
} } Thanks for any help.
} }
} } Stacey Andringa
} }
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (stefan.diller-at-t-online.de) from http://www.microscopy.com/MLFormMail.html on Wednesday, August 10, 2005 at 10:38:26
---------------------------------------------------------------------------

Email: stefan.diller-at-t-online.de
Name: Stefan Diller

Title-Subject: [Filtered] Soap structure

Question: Hello,
is anybody out there willing to share the secrets how to image soap structures in lubricants?
Or how to get the oil out of the soap structure for doing SEM work?
Is there any publication available in this field of work?
Please feel free to contact me offline.

Best regards,
Stefan Diller

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8, 12 -- From zaluzec-at-microscopy.com Wed Aug 10 20:00:19 2005
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Stefan,

One possible way is by using the Quantomix Wet-SEM
technology.

http://www.quantomix.com/

I haven't used their system, but it may be one
possible way to image lubricant soap structures.

Stu Smalinskas, P.E.
Metallurgist
SKF North American Technical Center
Plymouth, Michigan
(734) 414-6862

--- stefan diller wrote:

}
} Email: stefan.diller-at-t-online.de
} Name: Stefan Diller
}
} Title-Subject: [Filtered] Soap structure
}
} Question: Hello,
} is anybody out there willing to share the secrets
} how to image soap structures in lubricants?
} Or how to get the oil out of the soap structure for
} doing SEM work?
} Is there any publication available in this field of
} work?
} Please feel free to contact me offline.
}
} Best regards,
} Stefan Diller
}
}
} -----------------------------------------
} Stefan Diller - Photography
} Arndtstrasse 22
} D - 97072 Wuerzburg Germany
} ++49 - 931 - 7848700 Phone
} ++49 - 931 - 7848701 Fax
} ++49 - 175 - 717 70 51 Cell-Phone

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (estyer-at-uga.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, August 11, 2005 at 08:59:18
---------------------------------------------------------------------------

Email: estyer-at-uga.edu
Name: Eloise L. Styer

Organization: University of Georgia

Title-Subject: [Filtered] drying ethanol or acetone

Question: I would like to change our TEM tissue embeddment protocol to eliminate propylene oxide. Toward that end, what are some effective (and quick and easy...) methods to remove water from "100%" ethanol or acetone? We have an extremely humid building environment and I am sure there are many better techniques than my ancient method of adding the appropriate molecular sieve, swirling and letting stand until the fine particulates settle...

Thanks in advance.

Eloise---

Dr. Eloise L. Styer
Veterinary Diagnostic Lab
University of Georgia
43 Brighton Road
POB 1389
Tifton, GA 31793

Phone 229-386-3340
Fax 229-386-7128
e-mail estyer-at-uga.edu

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Dear Listers,

Many thanks to all of you who provided input on TEM
processing for agar encapsulated samples.

Also, the article by Paul Webster in Microscopy Today
shows insightful techniques to process small pellets,
samples. Thank you for pointing me to that article.

Have a nice day,

Claire

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For many years now we have been purchasing sealed, pint containers of
100% ethanol. We use freshly opened containers as the final ethanol.
After several openings, this is then considered as 95% ethanol and
used to produced the more dilute ethanol series. We have never had a
failed embedding due to water in the ethanol using this procedure.
And, yes, we are also in a humid environment.

} Email: estyer-at-uga.edu
} Name: Eloise L. Styer
}
} Organization: University of Georgia
}
} Title-Subject: [Filtered] drying ethanol or acetone
}
} Question: I would like to change our TEM tissue embeddment protocol
} to eliminate propylene oxide. Toward that end, what are some
} effective (and quick and easy...) methods to remove water from
} "100%" ethanol or acetone? We have an extremely humid building
} environment and I am sure there are many better techniques than my
} ancient method of adding the appropriate molecular sieve, swirling
} and letting stand until the fine particulates settle...
}
} Thanks in advance.
}
} Eloise---
}
} Dr. Eloise L. Styer
} Veterinary Diagnostic Lab
} University of Georgia
} 43 Brighton Road
} POB 1389
} Tifton, GA 31793
}
} Phone 229-386-3340
} Fax 229-386-7128
} e-mail estyer-at-uga.edu
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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--
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John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
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Carbondale, IL 62901 U.S.A.
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how does one make 100% etoh dryer? isn't it already
dry? or did i miss something in my organic chemistry?

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i know this, the original question was how to dry out
100% etoh and ethanol. yes of course 100% etoh will
absorb moisture from the air and how much will depend
on the humidity and lenght of time the bottle is open.
i would think in modern air conditioned buildings
would have a fairly low humidity.
john

--- Jan Factor {jfactor-at-ns.purchase.edu} wrote:

} 100% EtOH immediately begins to remove water from
} the air (in an attempt
} to dry out the atmosphere, which is exactly what it
} does when it dries
} tissues), so as soon as you open it, it is no longer
} 100%. Drying agents
} (such as copper sulfate) are usually added to sop up
} the water as it
} enters the solution.
} --Jan Factor
}
} ---------------------------------------
} Jan Robert Factor, Ph.D.
} Professor of Biology
} ---------------------------------------
} Natural Sciences
} Purchase College
} State University of New York
} 735 Anderson Hill Rd.
} Purchase, NY 10577
} USA
} ---------------------------------------
} Office Tel: 914-251-6659
} Office Fax: 914-251-6635
} E-mail: jfactor-at-ns.purchase.edu
} or- jan.factor-at-purchase.edu
} ---------------------------------------
}
}
} hoffpajo-at-yahoo.com wrote:
} }
}
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} }
} } how does one make 100% etoh dryer? isn't it
} already
} } dry? or did i miss something in my organic
} chemistry?
} }
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} (PDT)
} } 2, 18 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
} } 2, 18 -- Subject: drying ethanol
} } 2, 18 -- To: microscopy-at-microscopy.com
} } 2, 18 -- MIME-Version: 1.0
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} charset=iso-8859-1
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5, 19 -- Subject: Re: [Microscopy] drying ethanol
5, 19 -- To: Jan Factor {jfactor-at-ns.purchase.edu} , microscopy-at-microscopy.com
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ech-at-interchange.ubc.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, August 11, 2005 at 11:29:45
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Email: ech-at-interchange.ubc.ca
Name: Elaine Humphrey

Organization: UBC

Title-Subject: [Filtered] MListserver: microwave protocols for electron and light microscopy

Question: Hello Everyone
The microwave protocols website has moved from http://www.microwaveprep.org to
http://www.microwaveprotocols.ubc.ca

Debby Sherman did an awesome job organizing the original website but didn't have the time to maintain it and has passed it on to me since I have someone who maintains my website. (Note: my webmaster wanted to use CSS. If you have a Mac there are still some problems using Internet Explorer but it works just fine in Safari. If anyone has any experience with CSS bugs for Mac IE, please get in touch with me.)

We started to use a microwave four years ago. It became so popular we now have two.

For confocal and fluorescent microscopy where a protocol normally takes three hours (an hour in the primary antibody and an hour in the secondary), it typically takes a total of half an hour in the microwave. So researchers come in at 9 am and are on the confocal at 9.30 am. We usually find less background staining and the cells are fresher - especially when the primary staining is usually overnight.

For em processing that normally takes about three days, it is 2-3 hours in the microwave.

If it works conventionally, it will probably work in the microwave. However, the settings tend to be different for different specimens. What works for one doesn't necessarily work for another. Hence the Microwave Protocols website as a depository for different protocols and tips. For instance, a PhD student here was using the microwave for fluorescent staining of brain slices and found that staining 30 micron sections in eppendorf tubes stained better than 10-15 micron sections in a 24 well plate.

If you have a protocol that works for a particular specimen, please submit it to anyone of the reviewers. We have a review panel to check the protocols before they go up.

Elaine Humphrey, Director of the BioImaging Facility,
The University of British Columbia, Vancouver, CA
ech-at-interchange.ubc.ca

Kent McDonald, Director of the Electron Microscopy Lab,
University of California, Berkeley
klm-at-uclink4.berkeley.ed

Rick Webb, Centre for Microscopy and Microanalysis,
University of Queensland, Brisbane, AU
r.webb-at-uq.edu.au

Ronald Austin, Health Sciences Center,
Louisiana State University, Shreveport, LA
RAusti-at-lsuhsc.edu

Richard Giberson, Biomedical R&D,
Ted Pella, Inc., Redding, CA
rick_giberson-at-tedpella.com

Paul Webster, Ahmanson Advanced EM & Imaging Center,
House Ear Institute, Los Angeles, CA
pwebster-at-hei.org

Jonathan Day, Department of Biology,
California State University, Chico, Chico, CA
jday-at-csuchico.edu

If anyone wants to try using the microwave, there is going to be a demo/workshop in the next Scanning meeting to be held in Washington DC next April.

Elaine

--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada (2003-2005)
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca

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==============================Original Headers==============================
23, 12 -- From zaluzec-at-microscopy.com Thu Aug 11 18:43:31 2005
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (s2kdude-at-pacbell.net) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, August 11, 2005 at 13:10:47
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Email: s2kdude-at-pacbell.net
Name: willy

Title-Subject: [Filtered] MListserver: T-12 communication

Question: I need to know if anyone out there has had success in connecting an external PC that runs a ccd camera to a T-12 TEM. Is it possible to get communication such as mag and kV this way?

---------------------------------------------------------------------------

==============================Original Headers==============================
5, 12 -- From zaluzec-at-microscopy.com Thu Aug 11 18:44:17 2005
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Bplowman-at-pacific.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, August 11, 2005 at 13:23:32
---------------------------------------------------------------------------

Email: Bplowman-at-pacific.edu
Name: Barbara Plowman

Organization: Univ. of the Pacific/Arthur A. Dugoni School of Dentistry

Title-Subject: [Filtered] Sputtering from Gold Coin

Question: Dear Listserver,
Awhile back there was a string about gold sputtering using a gold coin. This was considerably cheaper than buying a new target. I don't know how to find these in the archives. Do any of you know how this was done or if you know where this information is located in the archives of the Listserver? I have a Hummer V. Thanks. Barbara Plowman P.S. I hope this isn't a repeat post!

Barbara Plowman
Univ. of the Pacific
Arthur A. Dugoni School of Dentistry
2155 Webster Rm 642
San Francisco, CA
Bplowman-at-pacific.edu
415-929-6692

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==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Thu Aug 11 18:44:54 2005
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Dear Barbara,
Although probably more expensive than using a gold coin, you pay not much
more than the current price for gold if you get your local jeweller to make
up a target. We get ours made somewhat thicker than specified so they last
longer. The donation of a sputter-coated insect or other item is usually
much appreciated too!
cheers,
rosemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia

} From: Bplowman-at-pacific.edu
} Reply-To: Bplowman-at-pacific.edu
} Date: Thu, 11 Aug 2005 18:47:47 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] viaWWW: Sputtering from Gold Coin
}
}
}
}
} ----------------------------------------------------------------------------
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} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted
} by (Bplowman-at-pacific.edu) from
} http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday,
} August 11, 2005 at 13:23:32
} ---------------------------------------------------------------------------
}
} Email: Bplowman-at-pacific.edu
} Name: Barbara Plowman
}
} Organization: Univ. of the Pacific/Arthur A. Dugoni School of Dentistry
}
} Title-Subject: [Filtered] Sputtering from Gold Coin
}
} Question: Dear Listserver,
} Awhile back there was a string about gold sputtering using a gold coin. This
} was considerably cheaper than buying a new target. I don't know how to find
} these in the archives. Do any of you know how this was done or if you know
} where this information is located in the archives of the Listserver? I have a
} Hummer V. Thanks. Barbara Plowman P.S. I hope this isn't a repeat post!
}
}
} Barbara Plowman
} Univ. of the Pacific
} Arthur A. Dugoni School of Dentistry
} 2155 Webster Rm 642
} San Francisco, CA
} Bplowman-at-pacific.edu
} 415-929-6692
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 11 18:44:54 2005
} 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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} 8, 12 -- Subject: viaWWW: Sputtering from Gold Coin
} 8, 12 -- Content-Type: text/plain; charset="us-ascii"
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==============================Original Headers==============================
3, 23 -- From Rosemary.White-at-csiro.au Thu Aug 11 22:31:30 2005
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3, 23 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0
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3, 23 -- Subject: Re: [Microscopy] viaWWW: Sputtering from Gold Coin
3, 23 -- From: Rosemary White {Rosemary.White-at-csiro.au}
3, 23 -- To: {Bplowman-at-pacific.edu} , {Microscopy-at-microscopy.com}
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yes i know, i know. i was responding to a poorly
written question that stated they wanted to dry out
100% etoh.

--- MICHAEL J DELANNOY {mdelann1-at-jhem.jhmi.edu} wrote:

}
} According to the Warner-Graham Co. (my supplier)
} the 100% ethanol is already molecular sieved so
} should
} be free of water when you open it. Of course
} humidity
} in the lab would contaminate opened bottles. If you
} want
} to molecular sieve this stock, about 1 inch of
} molecular
} sieve 3 A 4-8 mesh beads, let it settle (few days)
} until clear.
} My supplier did mention this might form ethylene
} dichloride,
} not sure how
}
} M Delannoy
} ----- Original Message -----
} From: hoffpajo-at-yahoo.com
} Date: Thursday, August 11, 2005 2:15 pm
} Subject: [Microscopy] drying ethanol
}
} }
} }
} }
} }
}
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} } AmericaTo Subscribe/Unsubscribe --
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} }
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} }
} } how does one make 100% etoh dryer? isn't it
} already
} } dry? or did i miss something in my organic
} chemistry?
} }
} } __________________________________________________
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} } Tired of spam? Yahoo! Mail has the best spam
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} } ==============================Original
} } Headers==============================2, 18 -- From
}
} } hoffpajo-at-yahoo.com Thu Aug 11 13:12:05 2005
} } 2, 18 -- Received: from web50201.mail.yahoo.com
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} with SMTP id
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} } Aug 2005 13:12:05 -0500
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{20050811181204.25965.qmail-at-web50201.mail.yahoo.com} 2,
} 18 --
} } Received: from [68.32.53.160] by
} web50201.mail.yahoo.com via HTTP;
} } Thu, 11 Aug 2005 11:12:04 PDT
} } 2, 18 -- Date: Thu, 11 Aug 2005 11:12:04 -0700
} (PDT)
} } 2, 18 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
} } 2, 18 -- Subject: drying ethanol
} } 2, 18 -- To: microscopy-at-microscopy.com
} } 2, 18 -- MIME-Version: 1.0
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__________________________________________________
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Tired of spam? Yahoo! Mail has the best spam protection around
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==============================Original Headers==============================
6, 19 -- From hoffpajo-at-yahoo.com Fri Aug 12 11:23:37 2005
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6, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 19 -- Subject: Re: [Microscopy] drying ethanol
6, 19 -- To: MICHAEL J DELANNOY {mdelann1-at-jhem.jhmi.edu} , microscopy-at-microscopy.com
6, 19 -- In-Reply-To: {5a42b3504e20.42fc7020-at-jhmimail.jhmi.edu}
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Could someone please tell me the height of the Gatan 3/8" diameter
mounting stubs for their Disc Grinder? I am making an adapter for our
Dimpler(R) for a customer who uses that hand grinder and the dimensions
are critical. Obviously in my new position, I don't have one on hand
(or in my hand). If you could also verify the diameters for both the
stainless steel and Pyrex(R) mounts it would be greatly appreciated.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

==============================Original Headers==============================
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4, 25 -- From: "Scott Walck" {walck-at-southbaytech.com}
4, 25 -- To: {Microscopy-at-microscopy.com}
4, 25 -- Subject: Disc grinder mounting stub dimensions
4, 25 -- Date: Fri, 12 Aug 2005 10:40:47 -0700
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Dear Scott,
My Gatan Disc Grinder 623 has two mounting stubs and one is about 3/8" long
and the other a little (~1/16") longer. They were probably both longer when
it was new, but of course they get ground down a little as you use the tool.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
X-from: {walck-at-southbaytech.com}
To: {mager-at-interchange.ubc.ca}
Sent: Friday, August 12, 2005 10:44 AM

I'm looking for a multi format negative scanner (like my now broken
Polaroid Sprintscan 45). Any recommendations? I'd like to be able to scan
35mm, 4"x5" negs and 99 mm x 81 mm (EM negs) and microscope slides.

Thanks,

De Wood
USDA ARS WRRC
800 Buchanan St.
Albany, CA 94710
(510) 559-5653

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MLFormMail.html on Saturday, August 13, 2005 at 04:06:04
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Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: Techutopia

Title-Subject: [Filtered] MListserver: Working ISI Mini-SEM Console/ TV Scan May Be Available.

Question: Hi,

As some of you may have noticed on Usenet, I've been developing a digital/pc replacement for the "bulky" console of the diminutive ISI Mini-SEM. I hope to be able to bring the 'scope around to primary and secondary schools in hopes of inspiring kids into pursuing science.

The project is almost finished and it's time to decide what to do with the console. It's temtpting to canabalize it for the ~$150 in AMP connectors I'll need to finish my project, but before I tore into it I wanted to see if there was any interest from someone with a viable 'scope and problematic console. Only the console, TV scan controller, and little B/W monitor is included: I need the external power supply.

I still have some testing and analysis to do, probably be about a month before it's ready to ship.

Contact me if you're interested,

-Jeff

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MLFormMail.html on Saturday, August 13, 2005 at 04:25:30
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Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: Techutopia

Title-Subject: [Filtered] MListserver: ISI Mini-SEM Supplies/Upgrades/Accessories Wanted

Question: Hi,

I'm interested in purchasing accessories, upgrades, crossgrades, and parts for ISI MINI-SEM units. In particular I'm looking for alternate chambers and stage
arrangements. I have the "straight-on" chamber based on right angles: curious about the chamber with the 45 degree cut for the controls.

Filaments, apertures, liners, any other running gear always appreciated.

The 'scope will probably spend about %50 of it's on-hours in primary and secondary schools for the next few years, at least.

Anyone with extra stuff, or who might have a lead on a repository, please e-mail.

-Jeff

-Jeff

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Hi

My experience with a wide range of sputter coaters would suggest that
provided the gold coin was in good electrical contact with the power supply
(perhaps glued to an old target with a conducting media) you should find
everything works well.

Good Luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
X-from: {Bplowman-at-pacific.edu}
To: {protrain-at-emcourses.com}
Sent: Friday, August 12, 2005 12:45 AM

I tried having this part repaired, but got scammed. Fortunately, I have the
part back, if not my money. If anyone has a source of ball bearings, I will
try and fix it myself. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu

-----Original Message-----
X-from: hbarwood-at-troy.edu [mailto:hbarwood-at-troy.edu]
Sent: Thursday, July 21, 2005 2:04 PM
To: hbarwood-at-troy.edu

I have an old Simplex microscope (actually Leitz,) with one of the massive
stands used for this type of measuring microscope. The micrometer height
adjustment went out on me years ago and I've just been using the threaded
height adjustor on the column to focus the scope. Now, I'm at a point where
I really need the fine adjustment. Is there anyone out there who can
disassemble the focusing rack that holds the scope and replace the small
ball bearings so it will function again? If anyone can help, please let me
know. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu

==============================Original Headers==============================
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Dear Colleagues,

The main relay (RE-1) on my Philips EM 300 has quit on me and I'm
looking for anyone who might have spare parts for the EM 300 (for
sale or donation). I have images of the relay that I can send offline
if you need help in identifying the part.

Thanks in advance for any assistance!

Steve
--
Stephen J. Beck
Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}

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a very quick even yahoo search for ball bearing
yielded a at least a few companyies, try this one for
ball bearings: www.bocabearings.com
and i have no vested interest in this company

--- hbarwood-at-troy.edu wrote:

}
}
}
}
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}
} I tried having this part repaired, but got scammed.
} Fortunately, I have the
} part back, if not my money. If anyone has a source
} of ball bearings, I will
} try and fix it myself. Thanks.
}
} Henry Barwood
} Associate Professor of Science, Earth Science
} Department of Math and Physics
} MSCX 312G
} Troy University
} Troy, Alabama 36082
} hbarwood-at-troy.edu
}
} -----Original Message-----
} X-from: hbarwood-at-troy.edu [mailto:hbarwood-at-troy.edu]
} Sent: Thursday, July 21, 2005 2:04 PM
} To: hbarwood-at-troy.edu
} Subject: [Microscopy] Mechanical repair help?
}
}
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}
} I have an old Simplex microscope (actually Leitz,)
} with one of the massive
} stands used for this type of measuring microscope.
} The micrometer height
} adjustment went out on me years ago and I've just
} been using the threaded
} height adjustor on the column to focus the scope.
} Now, I'm at a point where
} I really need the fine adjustment. Is there anyone
} out there who can
} disassemble the focusing rack that holds the scope
} and replace the small
} ball bearings so it will function again? If anyone
} can help, please let me
} know. Thanks.
}
} Henry Barwood
} Associate Professor of Science, Earth Science
} Department of Math and Physics
} MSCX 312G
} Troy University
} Troy, Alabama 36082
} hbarwood-at-troy.edu
}
}
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} Headers==============================
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} 2005
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} (scan.troy.edu
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Steve Chapman wrote:
===============================================================
My experience with a wide range of sputter coaters would suggest that
provided the gold coin was in good electrical contact with the power supply
(perhaps glued to an old target with a conducting media) you should find
everything works well.
===============================================================
Steve is correct if you were using a coin made of pure gold, probably better
than 0.999 purity.

But it has to have that high of a purity to work. Why? Because with gold
being so soft, most (monetary) coins (in fact all that I have ever heard of)
contain alloying elements that don't sputter so well (or at all). Hence,
once a little gold is sputtered, the surface becomes rich in these (left
over) alloying elements and sputtering stops. One does not need very much
of the typical alloying elements before such difficulties present
themselves. Therefore, in the general case, "gold" coins really don't work
very well. We have also seen some evidence of particulate contamination of
the samples from small metal inclusions that rain down on the sample once
freed from the gold matrix. The exception is the Canadian Gold Maple Leaf
which is advertised as being 0.9999 pure and that, should work quite nicely
provided it is not too thick (2.8 mm) to fit into your cathode holder.
However most coaters don't like cathodes that thick, and they are designed
to take cathode diameters larger than the 30 mm Maple Leaf.

If the reason why one is motivated to try using a coin is to save money, a
better approach might be to visit a jewelry workshop and you should be able
to get a gold foil of the needed purity. But then again it tends to not be
in the most desirable thickness and it also would have to be cut into the
needed disc diameter with some amount of waste foil (and some of the
"savings"). You would also lose the opportunity to return the left over
cathode for recycling (for a recycling discount) as is possible with some
of the microscopy supply vendors who routinely supply replacement gold
cathodes for coaters. It is the mechanical processing and "cookie cutting"
of the gold cathodes, and polishing, that results in replacement cathodes
from traditional sources to appear "higher" in price relative to the jewelry
store option.

Disclaimer: SPI Supplies offers replacement gold and other targets for
sputter coaters and we would have a vested interest in having prospective
customers purchase their replacement cathodes from firms like ours.

Chuck

===========================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (peterd-at-pmail.ntu.edu.sg) from http://www.microscopy.com/MLFormMail.html on Monday, August 15, 2005 at 00:13:04
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Email: peterd-at-pmail.ntu.edu.sg
Name: Peter Darmawan

Organization: Nanyang Technological University

Title-Subject: [Filtered] MListserver: Steps to XPS data Interpretation

Question: Dear All,

I am a new graduate student, switching from Mechanical Engineering to Materials engineering.

I need some help in interpreting XPS data which I got. So far I could not find any help on the internet with regard to the data interpretation and books on XPS is limited at my school library. Any help would be greatly appreciated.

Thank you,

Peter

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We used a Canadian Maple Leaf several years and have had great success. We
pounded it flat and used silver paste for the adhesive.

Alan Stone
ASTON

At 01:27 PM 8/14/2005, you wrote:

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Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com

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I think you sent your e'mail to the wrong person. I don't think I know
you.

kathleen

-----Original Message-----
X-from: c.jeffree-at-ed.ac.uk [mailto:c.jeffree-at-ed.ac.uk]
Sent: Tuesday, August 09, 2005 2:27 AM
To: Greer, Kathleen P

I don't care
Chris

----- Original Message -----
X-from: {luc-at-anaspec.co.za}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Tuesday, August 09, 2005 8:22 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (estyer-at-uga.edu) from http://www.microscopy.com/MLFormMail.html on Monday, August 15, 2005 at 08:36:14
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Email: estyer-at-uga.edu
Name: Eloise L. Styer

Organization: University of Georgia

Title-Subject: [Filtered] drying ethanol

Question: Dear All,

Thanks for your suggestions. They were all appreciated. Well, all that did not simply find fault with my reference to "100%" alcohol. And here I thought that putting the 100% in quotations would make it clear that I was not referring to absolutely 100% alcohol...

Eloise---

Dr. Eloise L. Styer
Veterinary Diagnostic Lab.
University of Georgia
43 Brighton Road
POB 1389
Tifton, GA 31793

Phone 229-386-3340
Fax 229-386-7128
Email estyer-at-uga.edu

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mkomboli-at-uno.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, August 15, 2005 at 10:36:18
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Email: mkomboli-at-uno.edu
Name: Mary Kombolias

Organization: University of New Orleans

Title-Subject: [Filtered] LR gold and benzoyl peroxide

Question: Hi! I was wondering how I should catalyze LR Gold with benzoyl peroxide. The LR Gold resin that I purchased did not come with any instructions on how to add the catalyst. The blurb in the Ted Pella catalogue only mentioned "benzoyl peroxide, 1%w/v." My question is, do I add the benzoyl peroxide to the entire bottle of LR Gold the same way the catalyst is added to the entire bottle of LR White resin or do I add the benzoyl peroxide to whatever aliquot of LR Gold resin I am using for each project?

Thanks!

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Mon Aug 15 19:04:22 2005
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try this for instructions
http://www.polysciences.com/shop/assets/datasheets/641.pdf

--- mkomboli-at-uno.edu wrote:

}
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} on Monday, August 15, 2005 at 10:36:18
}
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}
} Email: mkomboli-at-uno.edu
} Name: Mary Kombolias
}
} Organization: University of New Orleans
}
} Title-Subject: [Filtered] LR gold and benzoyl
} peroxide
}
} Question: Hi! I was wondering how I should catalyze
} LR Gold with benzoyl peroxide. The LR Gold resin
} that I purchased did not come with any instructions
} on how to add the catalyst. The blurb in the Ted
} Pella catalogue only mentioned "benzoyl peroxide,
} 1%w/v." My question is, do I add the benzoyl
} peroxide to the entire bottle of LR Gold the same
} way the catalyst is added to the entire bottle of LR
} White resin or do I add the benzoyl peroxide to
} whatever aliquot of LR Gold resin I am using for
} each project?
}
} Thanks!
}
}
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}
} ==============================Original
} Headers==============================
} 7, 12 -- From zaluzec-at-microscopy.com Mon Aug 15
} 19:04:22 2005
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} (mac22.zaluzec.com [206.69.208.22])
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} ESMTP id j7G04LY2006977
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} 7, 12 -- From: mkomboli-at-uno.edu (by way of
} MicroscopyListserver)
} 7, 12 -- Subject: viaWWW: LR gold and benzoyl
} peroxide
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__________________________________________________
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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Mon Aug 15 19:43:32 2005
5, 19 -- Received: from web50207.mail.yahoo.com (web50207.mail.yahoo.com [206.190.38.48])
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] viaWWW: LR gold and benzoyl peroxide
5, 19 -- To: mkomboli-at-uno.edu, microscopy-at-microscopy.com
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Subject is: Sydney/Seefeld cryo workshop

The third of the popular Seefeld in Sydney Workshops
on Cryotechniques in Electron Microscopy will be
held at the Electron Microscope Unit, University of
Sydney on 24th - 28th October 2005.

That means that there is just over one month to
go until priority registration closes on September
23rd. After that date a place cannot be guaranteed.

The course includes standard cryo-fixation techniques,
cryo-ultramicrotomy and immunogold labelling, and will
introduce the participants to nano-cryo techniques including
automated vitrification, advanced cryo-imaging, cryo-EM
tomography,single particle analysis, and electron
crystallography.

It assumes prior knowledge of basic specimen preparation
for electron microscopy, general ultramicrotomy and basic
antibody staining.

Course instructors include:
Dr Jan Leunissen, Managing Director Aurion and
Director R&D EM Unit, Otago University, NZ
Ross Boadle, Senior Scientist, E. M. Laboratory,
Westmead Millenium Inst. and ICPMR Westmead
Dr Ben Hankamer, Inst. for Molecular Bioscience,
University of Queensland
Assoc. Prof.Guy Cox, E.M.Unit, University of Sydney
Assoc. Prof.Filip Braet, Deputy Director, E.M. Unit,
University of Sydney
Anne Simpson, Specimen Preparation Manager,E.M. Unit,
University of Sydney
Graham Tranter, Emgrid Australia
Dr Teresa Dibbayawan, Leica Microsystems
Jocelyn Carpenter, Nanotechnology Systems

Equipment available will include Leica High Pressure Freezer,
Automatic Freeze Substitution and Cryo-ultramicrotomes and
FEI Vitrobot system.

Registration is $A1,000 (commercial) and $A850 (education),
which includes lunches and morning and afternoon tea

This course is run by NANO (Nanostructural Analysis Network
Organisation) and NANO subscribers receive a discounted rate.

We gratefully acknowlege the sponsorship of Leica, FEI and
Nanotechnology Systems.

For more details email
seefeld.cryo-at-emu.usyd.edu.au
or go to:
_______________________________________________________

http://www.nano.org.au http://www.emu.usyd.edu.au
_______________________________________________________

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12, 12 -- From: Guy Cox {guy-at-emu.usyd.edu.au} (by way of MicroscopyListserver)
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Dear Dr. Komboli,
Just add the required amount of Benzoyl peroxide in
an aliquot of LR gold (the amount you require) and
stir it. It would dissolve then you can embed your
samples and polymerise. I use about 50 mg for 5ml of
LR gold.
shashi singh
CCMB Hyderabad
INDIA

--- mkomboli-at-uno.edu wrote:

}
}
}
}
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} (NJZFM-ultra-55). It was submitted by
} (mkomboli-at-uno.edu) from
}
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} on Monday, August 15, 2005 at 10:36:18
}
---------------------------------------------------------------------------
}
} Email: mkomboli-at-uno.edu
} Name: Mary Kombolias
}
} Organization: University of New Orleans
}
} Title-Subject: [Filtered] LR gold and benzoyl
} peroxide
}
} Question: Hi! I was wondering how I should catalyze
} LR Gold with benzoyl peroxide. The LR Gold resin
} that I purchased did not come with any instructions
} on how to add the catalyst. The blurb in the Ted
} Pella catalogue only mentioned "benzoyl peroxide,
} 1%w/v." My question is, do I add the benzoyl
} peroxide to the entire bottle of LR Gold the same
} way the catalyst is added to the entire bottle of LR
} White resin or do I add the benzoyl peroxide to
} whatever aliquot of LR Gold resin I am using for
} each project?
}
} Thanks!
}
}
---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 7, 12 -- From zaluzec-at-microscopy.com Mon Aug 15
} 19:04:22 2005
} 7, 12 -- Received: from [206.69.208.22]
} (mac22.zaluzec.com [206.69.208.22])
} 7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j7G04LY2006977
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} Aug 2005 19:04:22 -0500
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} 7, 12 -- From: mkomboli-at-uno.edu (by way of
} MicroscopyListserver)
} 7, 12 -- Subject: viaWWW: LR gold and benzoyl
} peroxide
} 7, 12 -- Content-Type: text/plain;
} charset="us-ascii"
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==============================Original Headers==============================
5, 20 -- From shashis_99-at-yahoo.com Mon Aug 15 23:08:30 2005
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5, 20 -- From: shashi singh {shashis_99-at-yahoo.com}
5, 20 -- Subject: Re: [Microscopy] viaWWW: LR gold and benzoyl peroxide
5, 20 -- To: mkomboli-at-uno.edu
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Hello again listers,

A former colleague, now in another continent, has sent me the following
query. Could any of you please share your experiences on ANY of the aspects
here: if you feel there's the likelihood of a commercial flame war
developing, please send the reply to me only, but any thoughts of general
interest please reply to the Listserver.

Thanks for your attention.

* * * * * * * * * * * * * * *

I have some microscopy questions again, wondering if you can help? I am
going to buy an optical microscope for our institute, of course, my students
will use it more than others. I expect to use it in transmission Polarized
mode (for liquid crystal, polymer crystallization etc) and reflective
Nomarski interference contrast mode (for polymer surface and other surface
morphology) (still under consideration are the fluorescent mode combined
with our existing monochromator and CCD spectrometer)

I have contacted two companies, Leica and Olympus. However, as the price for
Leica is higher than Olympus, it is very hard for me to make a decision for
which company to go. I don't know if I can have some advice from you? I am
not familiar with Olympus and not sure the quality of it.

Leica has put on market many new models. The one I bought in the UK was a
Research type model. Leica here has an upgraded research model, but many new
low cost analytical models, cheaper on the body. At the moment, their
research model is still very expensive. I may have difficulty to afford it.
However, I am not sure of the quality of these analytical models. Leica
low cost polarized model has its analyser resolved only at 2-3 degree, but
in their research model the analyser can resolve to 0.1 degree. The
analytical model can also be fitted with reflective Interference Contrast
(via Smith reflector and DIC prisms)

I was told Olympus developed from biology microscopy and now is capable to
fit Nomarski Optics (they call it differential interference contrast DIC). I
have seen a research type Olympus microscope today. It seems quite good to
me. As I do not have sample to try, I checked only quality of the cross
polarizer. It seems the polarizer quality is good.

What Olympus argue that they can provide better quality of long working
distance lens than Leica to work with LINKAM hotstage. Leica provide me only
N PLAN lens for both polarized and interference contrast. Olympus suggest
these lenses are not good enough for interference contrast.

The choice of Lens seems very important. However, Leica Agents have not
advised me on that so far. I don't have must experience on choosing lens. Do
you also have any suggestions on that? I am looking forward to hearing from
you.

* * * * * * EOF * * * * * *

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------

==============================Original Headers==============================
14, 21 -- From hinmeigeng-at-hotmail.com Tue Aug 16 13:33:58 2005
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14, 21 -- Cc: R.H.Olley-at-reading.ac.uk
14, 21 -- Subject: Optical Microscopes: Leica v Olympus
14, 21 -- Date: Tue, 16 Aug 2005 18:33:57 +0000
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It was over 5 years ago that I shopped for a Pol Scope. But I think our
strategy will still work.

1. We collected a set of slides representing the type of things we would or
might want to see through the scope. In our case it included hairs, molds,
insects, crystals, etc.

2. We prepared an evaluation sheet with each feature we want to look at or
check, each specimen to examine, also the manufacturer, model demonstrated,
costs, etc.

3. We gave each available vendor a separate day to demo their scope using
our set of slides & any they supplied.

4. We had 2-3 evaluators who filled out an evaluation sheet for each scope
we looked at.

5. Later we compared the evaluations & discussed it as a group. We took
into account price, quality of scope, features, how well it worked with our
slide set, responsiveness & attitude of salespeople to our requests &
questions, availability & cost of service, warranty length, etc before
making our recommendations to our supervisor & lab director.

Hope that helps.

David A. Foran, chemist
Food and Drug Administration
Kansas City Elemental Analysis Lab
DFORAN-at-ORA.FDA.GOV
913-752-2170
913-752-2151 (fax)

-----Original Message-----
X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com]
Sent: Tuesday, August 16, 2005 1:36 PM
To: Foran, David A

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 16, 2005 at 13:47:01
---------------------------------------------------------------------------

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: University of Virginia

Title-Subject: [Filtered] LM Digital Camera Recommendations?

Question: Hey All,
I've decided to update the digital camera on our Olympus PME 3 invertedlight microscope. The digital camera that is currently attached is a Kodak DMC I (digital microscope camera). Any suggestions what to get that won't break the bank? I'm going for the best resolution for the buck. I've heard of people buying regular digital cameras and kits to attach to microscopes rather than the specialized microscope cameras; I don't know which gives better pictures/scheaper. I just need better resolution than what we currently have. Being able to control the camera from the computer and import directly into photoshop is nice also. Thanks in advance for the advice!
-Andrew Roelant
Graduate Research Assistant
Department of Materials Science and Engineering
University of Virginia

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16 14:39:15 2005
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i like the nikon D-70 digital camera. it can easily
fitted to most microscopes or as for my use a
telescope with a t mount adaptor.
john
--- amr2w-at-virginia.edu wrote:

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} Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (amr2w-at-virginia.edu) from
} http://www.microscopy.com/MLFormMail.html on
} Tuesday, August 16, 2005 at 13:47:01
}
---------------------------------------------------------------------------
}
} Email: amr2w-at-virginia.edu
} Name: Andrew Roelant
}
} Organization: University of Virginia
}
} Title-Subject: [Filtered] LM Digital Camera
} Recommendations?
}
} Question: Hey All,
} I've decided to update the digital camera on our
} Olympus PME 3 invertedlight microscope. The digital
} camera that is currently attached is a Kodak DMC I
} (digital microscope camera). Any suggestions what to
} get that won't break the bank? I'm going for the
} best resolution for the buck. I've heard of people
} buying regular digital cameras and kits to attach to
} microscopes rather than the specialized microscope
} cameras; I don't know which gives better
} pictures/scheaper. I just need better resolution
} than what we currently have. Being able to control
} the camera from the computer and import directly
} into photoshop is nice also. Thanks in advance for
} the advice!
} -Andrew Roelant
} Graduate Research Assistant
} Department of Materials Science and Engineering
} University of Virginia
}
}
---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16
} 14:39:15 2005
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} (mac22.zaluzec.com [206.69.208.22])
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} 6, 12 -- From: amr2w-at-virginia.edu (by way of
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} Recommendations?
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____________________________________________________
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6, 19 -- From hoffpajo-at-yahoo.com Tue Aug 16 14:44:05 2005
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We are interested in feedback from any group in the community who has
experience with plasma cleaning TEM samples prepared by ultramicrotomy. Our
specific samples are inorganic (mineral plus some carbonaceous material)
grains in epoxy ultramicrotomy sections suppoted on continuous carbon film
TEM grids. We would like to know whether contamination can be successfully
prevented, or removed, from these samples by plasma cleaning (while in the
TEM holder) without also causing destruction, alteration or volatilization
of the epoxy section and/or carbon support film.

Thanks,

Roy Christoffersen

SAIC

NASA Johnson Space Center EM Facilities

==============================Original Headers==============================
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8, 19 -- To: "Microscopy Listserver" {Microscopy-at-Microscopy.Com}
8, 19 -- Subject: TEM Sample Prep: Plasma Cleaning Ultramicrotomy Samples
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For years we have been using a Kodak DCS760 (actually a
Nikon F5 SLR body with Kodak CDD and electronics). The SLR's
are very nice because (1) they mount on the photo ports correctly
(i.e. F-mount, and otehrs), (2) they use the microscopes optics
alone, (3) and the SLR's have the better end (consumer/prosumer)
CCD's and electronics. Negative side of things is that unlike true
scieitific cameras the exposure systems are not setup to handle
Light Microsopy type imaging very well and so a little fiddling is
required.

We just got a new Nikon D-50 (very similar to the D-70, CCD
noise seems a little better, and $100 lower cost). With the Nikon
Capture software it can be used in "tethered" mode and driven
completely by the computer. Now, when I say we just got, I really
mean that, the box arrived this morning and we haven't installed it
yet. Give me a couple of days and I will give a report back.

As for breaking the bank: Nikon D-50 body $720, Capture
software (needed for tethered operation) $100, and right angle
viewfinder $185.

Right angle view finder: Very VERY helpful for upright
microscopes, but may not be needed / useful if you can use the
front camera port on an inverted scope.

More info on the Nikon & other digital camers take a look at:

http://www.dpreview.com/reviews/specs/Nikon/

(BTW: I do not sell cameras or anything else nor have any financial
interests in digital cameras or Nikon)

On 16 Aug 2005, at 14:40, amr2w-at-virginia.edu wrote:

}
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}
} ----------------------------------------------------------------------------
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} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 16, 2005 at 13:47:01
} ---------------------------------------------------------------------------
}
} Email: amr2w-at-virginia.edu
} Name: Andrew Roelant
}
} Organization: University of Virginia
}
} Title-Subject: [Filtered] LM Digital Camera Recommendations?
}
} Question: Hey All,
} I've decided to update the digital camera on our Olympus PME 3 invertedlight microscope. The digital camera that is currently attached is a Kodak DMC I (digital microscope camera). Any suggestions what to get that won't break the bank? I'm going for the best resolution for the buck. I've
heard of people buying regular digital cameras and kits to attach to microscopes rather than the specialized microscope cameras; I don't know which gives better pictures/scheaper. I just need better resolution than what we currently have. Being able to control the camera from the computer and
import directly into photoshop is nice also. Thanks in advance for the advice!
} -Andrew Roelant
} Graduate Research Assistant
} Department of Materials Science and Engineering
} University of Virginia
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16 14:39:15 2005
} 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j7GJdEnj010005
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} 6, 12 -- To: microscopy-at-microscopy.com
} 6, 12 -- From: amr2w-at-virginia.edu (by way of MicroscopyListserver)
} 6, 12 -- Subject: viaWWW: LM Digital Camera Recommendations?
} 6, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

==============================Original Headers==============================
15, 23 -- From edelmare-at-muohio.edu Wed Aug 17 10:14:14 2005
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15, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
15, 23 -- To: amr2w-at-virginia.edu, microscopy-at-Microscopy.com
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for high quality images everything depends on the
pixels. the more pixels the better the image.
as for exposeures, everyone needs to remember all
cameras ""see"" things as an 18% grey card. so all
images need to be adjusted.
and yes to all those of you about to jump on me i know
this is simplistic.
--- edelmare-at-muohio.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} For years we have been using a Kodak DCS760
} (actually a
} Nikon F5 SLR body with Kodak CDD and electronics).
} The SLR's
} are very nice because (1) they mount on the photo
} ports correctly
} (i.e. F-mount, and otehrs), (2) they use the
} microscopes optics
} alone, (3) and the SLR's have the better end
} (consumer/prosumer)
} CCD's and electronics. Negative side of things is
} that unlike true
} scieitific cameras the exposure systems are not
} setup to handle
} Light Microsopy type imaging very well and so a
} little fiddling is
} required.
}
} We just got a new Nikon D-50 (very similar to the
} D-70, CCD
} noise seems a little better, and $100 lower cost).
} With the Nikon
} Capture software it can be used in "tethered" mode
} and driven
} completely by the computer. Now, when I say we just
} got, I really
} mean that, the box arrived this morning and we
} haven't installed it
} yet. Give me a couple of days and I will give a
} report back.
}
} As for breaking the bank: Nikon D-50 body $720,
} Capture
} software (needed for tethered operation) $100, and
} right angle
} viewfinder $185.
}
} Right angle view finder: Very VERY helpful for
} upright
} microscopes, but may not be needed / useful if you
} can use the
} front camera port on an inverted scope.
}
} More info on the Nikon & other digital camers take a
} look at:
}
} http://www.dpreview.com/reviews/specs/Nikon/
}
}
} (BTW: I do not sell cameras or anything else nor
} have any financial
} interests in digital cameras or Nikon)
}
}
} On 16 Aug 2005, at 14:40, amr2w-at-virginia.edu wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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}
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} }
}
----------------------------------------------------------------------------
} }
} } Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (amr2w-at-virginia.edu) from
} http://www.microscopy.com/MLFormMail.html on
} Tuesday, August 16, 2005 at 13:47:01
} }
}
---------------------------------------------------------------------------
} }
} } Email: amr2w-at-virginia.edu
} } Name: Andrew Roelant
} }
} } Organization: University of Virginia
} }
} } Title-Subject: [Filtered] LM Digital Camera
} Recommendations?
} }
} } Question: Hey All,
} } I've decided to update the digital camera on
} our Olympus PME 3 invertedlight microscope. The
} digital camera that is currently attached is a Kodak
} DMC I (digital microscope camera). Any suggestions
} what to get that won't break the bank? I'm going for
} the best resolution for the buck. I've
} heard of people buying regular digital cameras and
} kits to attach to microscopes rather than the
} specialized microscope cameras; I don't know which
} gives better pictures/scheaper. I just need better
} resolution than what we currently have. Being able
} to control the camera from the computer and
} import directly into photoshop is nice also. Thanks
} in advance for the advice!
} } -Andrew Roelant
} } Graduate Research Assistant
} } Department of Materials Science and Engineering
} } University of Virginia
} }
} }
}
---------------------------------------------------------------------------
} }
} } ==============================Original
} Headers==============================
} } 6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16
} 14:39:15 2005
} } 6, 12 -- Received: from [206.69.208.22]
} (mac22.zaluzec.com [206.69.208.22])
} } 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8)
} with ESMTP id j7GJdEnj010005
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} Aug 2005 14:39:15 -0500
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} } 6, 12 -- To: microscopy-at-microscopy.com
} } 6, 12 -- From: amr2w-at-virginia.edu (by way of
} MicroscopyListserver)
} } 6, 12 -- Subject: viaWWW: LM Digital Camera
} Recommendations?
} } 6, 12 -- Content-Type: text/plain;
} charset="us-ascii"
} } ==============================End of -
} Headers==============================
}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
} ==============================Original
} Headers==============================
} 15, 23 -- From edelmare-at-muohio.edu Wed Aug 17
} 10:14:14 2005
} 15, 23 -- Received: from mulnx12.mcs.muohio.edu
} (mulnx12.mcs.muohio.edu [134.53.6.67])
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} with ESMTP id j7HFEEWY015687
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} Aug 2005 10:14:14 -0500
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} (mulnx24.mcs.muohio.edu [134.53.6.11])
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} 15, 23 -- Wed, 17 Aug 2005 11:14:09 -0400
} 15, 23 -- From: "Richard E. Edelmann"
} {edelmare-at-muohio.edu}
} 15, 23 -- To: amr2w-at-virginia.edu,
} microscopy-at-Microscopy.com
} 15, 23 -- Date: Wed, 17 Aug 2005 11:14:08 -0400
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} Digital Camera Recommendations?
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}
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__________________________________________________
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] Re: viaWWW: LM Digital Camera Recommendations?
5, 19 -- To: edelmare-at-muohio.edu, microscopy-at-microscopy.com
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The Center for Nanophase Materials Science (CNMS) and the Condensed Matter Sciences Division of Oak Ridge National Laboratory invite applications for an experimental postdoctoral position research in development and application of advanced scanning probe microscopies (SPM) to complex materials and to support the user-initiated nanoscience research program of the CNMS. The successful applicant should have demonstrated experience in the application, development, and interpretation of modern SPM techniques, such as acoustic, electromechanical, electrochemical, transport imaging or imaging in a liquid environment.� The applicant should have a Ph.D. in materials science, physics, or related field, and be capable of interacting with a wide range of users probing the nanoscale properties of a diverse range of materials including polymers and other soft materials, semiconductors, and biological systems. This position provides an opportunity to take advantage of new state-of-the-art facilities at the Center for Nanophase Materials Science, including a NanoFabrication Center and advanced scanning based probes and to interact with ongoing programs in the Low Dimensional Materials by Design and first-principles theory groups at ORNL. The complete information on this position is available on the CNMS web-site: http://cnms.ornl.gov/postdoc_research/CNMS_PS.shtm
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I think this post is too simplistic. Don't play the Pixel game when you buy a scientific camera. For example: If you buy an 8 Mpixel camera to work at high magnification on your microscope, you are wasting money and storage. The resolution will be limited by the microscope, and not by the number of pixels of the camera. Likewise, if you are doing Fluorescence, a camera with fewer pixels can be more sensitive and thus be superior to a camera with gobs of pixels.

Also, please explain your comment that "all cameras ""see"" things as an 18% grey card".

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
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-----Original Message-----
X-from: hoffpajo-at-yahoo.com [mailto:hoffpajo-at-yahoo.com]
Sent: Wednesday, August 17, 2005 9:28 AM
To: Mike Bode

for high quality images everything depends on the pixels. the more pixels the better the image.
as for exposeures, everyone needs to remember all cameras ""see"" things as an 18% grey card. so all images need to be adjusted.
and yes to all those of you about to jump on me i know this is simplistic.
--- edelmare-at-muohio.edu wrote:

}
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} For years we have been using a Kodak DCS760 (actually a Nikon F5 SLR
} body with Kodak CDD and electronics).
} The SLR's
} are very nice because (1) they mount on the photo ports correctly
} (i.e. F-mount, and otehrs), (2) they use the microscopes optics alone,
} (3) and the SLR's have the better end
} (consumer/prosumer)
} CCD's and electronics. Negative side of things is that unlike true
} scieitific cameras the exposure systems are not setup to handle Light
} Microsopy type imaging very well and so a little fiddling is required.
}
} We just got a new Nikon D-50 (very similar to the D-70, CCD noise
} seems a little better, and $100 lower cost).
} With the Nikon
} Capture software it can be used in "tethered" mode and driven
} completely by the computer. Now, when I say we just got, I really
} mean that, the box arrived this morning and we haven't installed it
} yet. Give me a couple of days and I will give a report back.
}
} As for breaking the bank: Nikon D-50 body $720, Capture software
} (needed for tethered operation) $100, and right angle viewfinder
} $185.
}
} Right angle view finder: Very VERY helpful for upright microscopes,
} but may not be needed / useful if you can use the front camera port on
} an inverted scope.
}
} More info on the Nikon & other digital camers take a look at:
}
} http://www.dpreview.com/reviews/specs/Nikon/
}
}
} (BTW: I do not sell cameras or anything else nor have any financial
} interests in digital cameras or Nikon)
}
}
} On 16 Aug 2005, at 14:40, amr2w-at-virginia.edu wrote:
}
} }
} }
} }
} }
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} } Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (amr2w-at-virginia.edu) from
} http://www.microscopy.com/MLFormMail.html on Tuesday, August 16, 2005
} at 13:47:01
} }
}
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} }
} } Email: amr2w-at-virginia.edu
} } Name: Andrew Roelant
} }
} } Organization: University of Virginia
} }
} } Title-Subject: [Filtered] LM Digital Camera
} Recommendations?
} }
} } Question: Hey All,
} } I've decided to update the digital camera on
} our Olympus PME 3 invertedlight microscope. The digital camera that is
} currently attached is a Kodak DMC I (digital microscope camera). Any
} suggestions what to get that won't break the bank? I'm going for the
} best resolution for the buck. I've heard of people buying regular
} digital cameras and kits to attach to microscopes rather than the
} specialized microscope cameras; I don't know which gives better
} pictures/scheaper. I just need better resolution than what we
} currently have. Being able to control the camera from the computer and
} import directly into photoshop is nice also. Thanks in advance for the
} advice!
} } -Andrew Roelant
} } Graduate Research Assistant
} } Department of Materials Science and Engineering University of
} } Virginia
} }
} }
}
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} }
} } ==============================Original
} Headers==============================
} } 6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16
} 14:39:15 2005
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} } of
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} Recommendations?
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}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
} ==============================Original
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} 10:14:14 2005
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} {edelmare-at-muohio.edu}
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==============================Original Headers==============================
16, 23 -- From Mike.Bode-at-soft-imaging.net Wed Aug 17 10:49:15 2005
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Roy

We routinely plasma clean holey/continuous carbon films with
particles on them it works
fine here at ANL. I've only done a few (i.e. { 5) microtomed
samples, but have not had
any problems with them, but that is not a significant number of
samples to gauge the
effectiveness.

The key here is to recognize, as you have apparently already done,
that the reactive plasma obviously will attack the carbon in your
support as well as the source of hydrocarbon contamination. I have found
that the hydrocarbon, not surprizingly is more reactive than, for
example, the more
stable carbon support films. The key here is to tailor your gas composition
as well as to adjust the power levels of your plasma. At ANL, for
carbon support films, we
use Argon gas only and at a low power setting of ~ 5-10 W for ~ 10 minutes
in our SBT PC-2000 plasma cleaner. In my experience, for these type of samples,
you should keep oxygen to a minimum as this will rapidly attach all
carbon. Basically,
we have found that there will be enough residual oxygen released by
the Argon plasma
to attack the organic contamination, assuming the specimen is not
heavily coated.

Of course, not all "carbon support films", are created equal and
some have more
hydrocarbon than others, so you will need to test the receipe for your films
as well as for the model of plasma cleaner and its settings
as each manufactureres unit will vary in efficacy. It is best here to make
a few sacrifical films and tune your settings to minimze the reaction on your
support films.

Nestor
Your Friendly Neighborhood SysOp

Disclaimer: My employer Argonne National Lab holds the basic patent
on TEM/SEM plasma
cleaning technology and licenses this to manufacturers of commerical units.

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--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================

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unless i have forgetten basics of camera exposures,
which is possible i am getting old. the metter in the
camera takes all the light falling on it and
"averages" it. it assumes the image to be aboout 18%
grey card. if you don't have one i can send you one of
mine. i use them to handle high contrast subjects.
--- Mike.Bode-at-soft-imaging.net wrote:

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} I think this post is too simplistic. Don't play the
} Pixel game when you buy a scientific camera. For
} example: If you buy an 8 Mpixel camera to work at
} high magnification on your microscope, you are
} wasting money and storage. The resolution will be
} limited by the microscope, and not by the number of
} pixels of the camera. Likewise, if you are doing
} Fluorescence, a camera with fewer pixels can be more
} sensitive and thus be superior to a camera with gobs
} of pixels.
}
} Also, please explain your comment that "all cameras
} ""see"" things as an 18% grey card".
}
} mike
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:� (888) FIND SIS
} �� (303) 234-9270
} fax:�� (303) 234-9271
} email:� mailto:info-at-soft-imaging.com
} web:�� http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: hoffpajo-at-yahoo.com
} [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 9:28 AM
} To: Mike Bode
} Subject: [Microscopy] viaWWW: LM Digital Camera
} Recommendations?
}
}
}
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again unless i am out of it isn't the highest
resolution possible to be about 200nm in a light
microscope? and pixel resolution to be between 3 to
8um, i could be wrong. again very simplistic. so i
guess i should hang onto my D-70.
--- Mike.Bode-at-soft-imaging.net wrote:

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}
} I think this post is too simplistic. Don't play the
} Pixel game when you buy a scientific camera. For
} example: If you buy an 8 Mpixel camera to work at
} high magnification on your microscope, you are
} wasting money and storage. The resolution will be
} limited by the microscope, and not by the number of
} pixels of the camera. Likewise, if you are doing
} Fluorescence, a camera with fewer pixels can be more
} sensitive and thus be superior to a camera with gobs
} of pixels.
}
} Also, please explain your comment that "all cameras
} ""see"" things as an 18% grey card".
}
} mike
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:� (888) FIND SIS
} �� (303) 234-9270
} fax:�� (303) 234-9271
} email:� mailto:info-at-soft-imaging.com
} web:�� http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: hoffpajo-at-yahoo.com
} [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 9:28 AM
} To: Mike Bode
} Subject: [Microscopy] viaWWW: LM Digital Camera
} Recommendations?
}
}
}
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200 nm is proabaly a bit optimistic, but let's go with that value. If you use a 100x lens, the 200 nm spot will be enlarged to 20 microns. If you use an 8 Mpixel camera with a pixel size of 2 microns or so, you are oversampling by a large factor.

Now, there are compelling reasons to buy an 8 Mpixel camera, but the number of pixels is not the only parameter you should use to decide on a camera.

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:� (888) FIND SIS
�� (303) 234-9270
fax:�� (303) 234-9271
email:� mailto:info-at-soft-imaging.com
web:�� http://www.soft-imaging.com
===================================

-----Original Message-----
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Sent: Wednesday, August 17, 2005 10:30 AM
To: Mike Bode; microscopy-at-microscopy.com

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Hmmm, you must be thinking on film cameras. In digital cameras, you don't have an extra sensor for exposure time setting. The imaging sensor itelf provides the information that you need. This allows a much better control of the exposure. For example you could take the information from the live image that is displayed on the camera or monitor, and find the maximum and minimum exposure pixels and then calculate a new exposure time to maximize signal, or minimize noise, etc. You can do that for the entire sensor, only use a certain spot, average over several spots...

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:� (888) FIND SIS
�� (303) 234-9270
fax:�� (303) 234-9271
email:� mailto:info-at-soft-imaging.com
web:�� http://www.soft-imaging.com
===================================

-----Original Message-----
X-from: hoffpajo-at-yahoo.com [mailto:hoffpajo-at-yahoo.com]
Sent: Wednesday, August 17, 2005 10:07 AM
To: Mike Bode

unless i have forgetten basics of camera exposures, which is possible i am getting old. the metter in the camera takes all the light falling on it and "averages" it. it assumes the image to be aboout 18% grey card. if you don't have one i can send you one of mine. i use them to handle high contrast subjects.
--- Mike.Bode-at-soft-imaging.net wrote:

}
}
}
}
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} America To Subscribe/Unsubscribe --
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}
} I think this post is too simplistic. Don't play the Pixel game when
} you buy a scientific camera. For
} example: If you buy an 8 Mpixel camera to work at high magnification
} on your microscope, you are wasting money and storage. The resolution
} will be limited by the microscope, and not by the number of pixels of
} the camera. Likewise, if you are doing Fluorescence, a camera with
} fewer pixels can be more sensitive and thus be superior to a camera
} with gobs of pixels.
}
} Also, please explain your comment that "all cameras ""see"" things as
} an 18% grey card".
}
} mike
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:� (888) FIND SIS
} �� (303) 234-9270
} fax:�� (303) 234-9271
} email:� mailto:info-at-soft-imaging.com
} web:�� http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: hoffpajo-at-yahoo.com
} [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 9:28 AM
} To: Mike Bode
} Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?
}
}
}
}
}
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that is the reason i like the d-70. it allows you to
sample the exposure over several spots. i also have
software purchased the adobe creative suite 2. it alos
for a great deal of control over the final image, such
as image bracking. i highly recomend it.

well i learned photography with a film camrea. i still
have several including a large format camera.
i have no vested interest in either nikon or adobe.
just like them both.

--- Mike.Bode-at-soft-imaging.net wrote:

}
}
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}
} Hmmm, you must be thinking on film cameras. In
} digital cameras, you don't have an extra sensor for
} exposure time setting. The imaging sensor itelf
} provides the information that you need. This allows
} a much better control of the exposure. For example
} you could take the information from the live image
} that is displayed on the camera or monitor, and find
} the maximum and minimum exposure pixels and then
} calculate a new exposure time to maximize signal, or
} minimize noise, etc. You can do that for the entire
} sensor, only use a certain spot, average over
} several spots...
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:� (888) FIND SIS
} �� (303) 234-9270
} fax:�� (303) 234-9271
} email:� mailto:info-at-soft-imaging.com
} web:�� http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: hoffpajo-at-yahoo.com
} [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 10:07 AM
} To: Mike Bode
} Subject: [Microscopy] viaWWW: LM Digital Camera
} Recommendations?
}
}
}
}
}
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Supposing the resolution is 200 nm, that is less than half a wavelength of
light. That might be attainable given a lens (and other optics) of
sufficiently high NA. Those are generally the higher power objectives. My
basic 40x lens has an NA of only 0.63, so I won't get such good resolution.
But suppose we could get 200 nm out of a 100x objective.

200 nm resolution means we would need pixels every 100 nm or so. I
generally figure prints of 120 mm or so wide. That means the width of my
image will be about 120 um at 1000x. That means I will need 1200 pixels
across my image for a 100-nm pixel spacing. That isn't very many pixels in
today's terms. My old 1.3 megapixel Pixera can handle that.

You might say that the extra pixels allow you to take lower magnification
images and still record up to the resolution limit. First, what is the
resolution for that set of lenses? The NA and resolution falls off with a
drop in magnification. There may not be detail to record with the extra
pixels.

Having said all that, 3 megapixel consumer cameras are practically throw
away items now. For that matter, so are 40 GB disk drives. It doesn't hurt
to add a few more pixels (and MB) to be safe. However, I think a lot of
people are quick to take high-pixel images for which they never will use
the information contained there. They just provide a demand for more and
bigger hard drives.

I am going to have to run a comparison here one of these days. I have a gut
feeling that lower pixel number cameras may be more sensitive in low-light
situations than their higher pixel cousins. (I think that is what Mike Bode
suggested.) I want to do a side-by-side comparison to show myself and
others. I will grant that higher pixel cameras can record more detail in
their regular photographic mode under sufficient light. I just don't know
that they have the same edge in the lab.

Warren Straszheim
(adding my name here so you don't have to check out the address line above
or below)

At 11:31 AM 08/17/05, John H. wrote:

} again unless i am out of it isn't the highest
} resolution possible to be about 200nm in a light
} microscope? and pixel resolution to be between 3 to
} 8um, i could be wrong. again very simplistic. so i
} guess i should hang onto my D-70.
} --- Mike.Bode-at-soft-imaging.net wrote:
}
} }
} }
} }
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
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} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} } I think this post is too simplistic. Don't play the
} } Pixel game when you buy a scientific camera. For
} } example: If you buy an 8 Mpixel camera to work at
} } high magnification on your microscope, you are
} } wasting money and storage. The resolution will be
} } limited by the microscope, and not by the number of
} } pixels of the camera. Likewise, if you are doing
} } Fluorescence, a camera with fewer pixels can be more
} } sensitive and thus be superior to a camera with gobs
} } of pixels.
} }
} } Also, please explain your comment that "all cameras
} } ""see"" things as an 18% grey card".
} }
} } mike
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 12596 West Bayaud Avenue
} } Suite 300
} } Lakewood, CO 80228
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} } -----Original Message-----
} } X-from: hoffpajo-at-yahoo.com
} } [mailto:hoffpajo-at-yahoo.com]
} } Sent: Wednesday, August 17, 2005 9:28 AM
} } To: Mike Bode
} } Subject: [Microscopy] viaWWW: LM Digital Camera
} } Recommendations?
} }
} }
} }
} }
} }
} ----------------------------------------------------------------------------
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} } Microscopy Society of America To
} } Subscribe/Unsubscribe --
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} }
} }
} } for high quality images everything depends on the
} } pixels. the more pixels the better the image.
} } as for exposeures, everyone needs to remember all
} } cameras ""see"" things as an 18% grey card. so all
} } images need to be adjusted.
} } and yes to all those of you about to jump on me i
} } know this is simplistic.
} } --- edelmare-at-muohio.edu wrote:
} }
} } }
} } }
} } }
} } }
} }
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} }
} ----------------------------------------------------------------------------
} } }
} } } For years we have been using a Kodak DCS760
} } (actually a Nikon F5 SLR
} } } body with Kodak CDD and electronics).
} } } The SLR's
} } } are very nice because (1) they mount on the photo
} } ports correctly
} } } (i.e. F-mount, and otehrs), (2) they use the
} } microscopes optics alone,
} } } (3) and the SLR's have the better end
} } } (consumer/prosumer)
} } } CCD's and electronics. Negative side of things is
} } that unlike true
} } } scieitific cameras the exposure systems are not
} } setup to handle Light
} } } Microsopy type imaging very well and so a little
} } fiddling is required.
} } }
} } } We just got a new Nikon D-50 (very similar to the
} } D-70, CCD noise
} } } seems a little better, and $100 lower cost).
} } } With the Nikon
} } } Capture software it can be used in "tethered" mode
} } and driven
} } } completely by the computer. Now, when I say we
} } just got, I really
} } } mean that, the box arrived this morning and we
} } haven't installed it
} } } yet. Give me a couple of days and I will give a
} } report back.
} } }
} } } As for breaking the bank: Nikon D-50 body $720,
} } Capture software
} } } (needed for tethered operation) $100, and right
} } angle viewfinder
} } } $185.
} } }
} } } Right angle view finder: Very VERY helpful for
} } upright microscopes,
} } } but may not be needed / useful if you can use the
} } front camera port on
} } } an inverted scope.
} } }
} } } More info on the Nikon & other digital camers take
} } a look at:
} } }
} } } http://www.dpreview.com/reviews/specs/Nikon/
} } }
} } }
} } } (BTW: I do not sell cameras or anything else nor
} } have any financial
} } } interests in digital cameras or Nikon)

==============================Original Headers==============================
9, 20 -- From wesaia-at-iastate.edu Wed Aug 17 12:05:26 2005
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9, 20 -- From: Warren E Straszheim {wesaia-at-iastate.edu}
9, 20 -- Subject: Re: [Microscopy] viaWWW: LM Digital Camera Recommendations?
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well i think the money spent on a camera that will
grow with the applications at a reasonable price is
the way to go. the nikon will allow you to adjust the
exposure and quality of the final image. much also
depends on how much enlargement you need before you
begin to see the pixels.
i firmly believe that digital is the wave of the
future. you can get a nikon d-70 for under $1000
these days. and if you wish it is vesatal enough to be
used for macro photagraphy and making slides for
presentations.
again i have no vested interest in nikon. so hold the
flames on that issue please
john

--- Mike.Bode-at-soft-imaging.net wrote:

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} 200 nm is proabaly a bit optimistic, but let's go
} with that value. If you use a 100x lens, the 200 nm
} spot will be enlarged to 20 microns. If you use an 8
} Mpixel camera with a pixel size of 2 microns or so,
} you are oversampling by a large factor.
}
} Now, there are compelling reasons to buy an 8 Mpixel
} camera, but the number of pixels is not the only
} parameter you should use to decide on a camera.
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:� (888) FIND SIS
} �� (303) 234-9270
} fax:�� (303) 234-9271
} email:� mailto:info-at-soft-imaging.com
} web:�� http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: john hoffpauir [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 10:30 AM
} To: Mike Bode; microscopy-at-microscopy.com
} Subject: Re: [Microscopy] RE: viaWWW: LM Digital
} Camera Recommendations?
}
} again unless i am out of it isn't the highest
} resolution possible to be about 200nm in a light
} microscope? and pixel resolution to be between 3 to
} 8um, i could be wrong. again very simplistic. so i
} guess i should hang onto my D-70.
} --- Mike.Bode-at-soft-imaging.net wrote:
}
} }
} }
} }
} }
}
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} }
} } I think this post is too simplistic. Don't play
} the Pixel game when
} } you buy a scientific camera. For
} } example: If you buy an 8 Mpixel camera to work at
} high magnification
} } on your microscope, you are wasting money and
} storage. The resolution
} } will be limited by the microscope, and not by the
} number of pixels of
} } the camera. Likewise, if you are doing
} Fluorescence, a camera with
} } fewer pixels can be more sensitive and thus be
} superior to a camera
} } with gobs of pixels.
} }
} } Also, please explain your comment that "all
} cameras ""see"" things as
} } an 18% grey card".
} }
} } mike
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 12596 West Bayaud Avenue
} } Suite 300
} } Lakewood, CO 80228
} } ===================================
} } phone:� (888) FIND SIS
} } �� (303) 234-9270
} } fax:�� (303) 234-9271
} } email:� mailto:info-at-soft-imaging.com
} } web:�� http://www.soft-imaging.com
} } ===================================
} }
} }
} } -----Original Message-----
} } X-from: hoffpajo-at-yahoo.com
} } [mailto:hoffpajo-at-yahoo.com]
} } Sent: Wednesday, August 17, 2005 9:28 AM
} } To: Mike Bode
} } Subject: [Microscopy] viaWWW: LM Digital Camera
} Recommendations?
} }
} }
} }
} }
} }
}
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} } Microscopy Society of America To
} } Subscribe/Unsubscribe --
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} }
} }
} } for high quality images everything depends on the
} } pixels. the more pixels the better the image.
} } as for exposeures, everyone needs to remember all
} } cameras ""see"" things as an 18% grey card. so all
} } images need to be adjusted.
} } and yes to all those of you about to jump on me i
} } know this is simplistic.
} } --- edelmare-at-muohio.edu wrote:
} }
} } }
} } }
} } }
} } }
} }
}
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} } Microscopy Society of
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} } }
} }
}
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} } }
} }
}
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} } }
} } } For years we have been using a Kodak DCS760
} } (actually a Nikon F5 SLR
} } } body with Kodak CDD and electronics).
} } } The SLR's
} } } are very nice because (1) they mount on the
} photo
} } ports correctly
} } } (i.e. F-mount, and otehrs), (2) they use the
} } microscopes optics alone,
} } } (3) and the SLR's have the better end
} } } (consumer/prosumer)
} } } CCD's and electronics. Negative side of things
} is
} } that unlike true
} } } scieitific cameras the exposure systems are not
} } setup to handle Light
} } } Microsopy type imaging very well and so a little
} } fiddling is required.
} } }
} } } We just got a new Nikon D-50 (very similar to
} the
} } D-70, CCD noise
} } } seems a little better, and $100 lower cost).
} } } With the Nikon
} } } Capture software it can be used in "tethered"
} mode
} } and driven
} } } completely by the computer. Now, when I say we
} } just got, I really
} } } mean that, the box arrived this morning and we
} } haven't installed it
} } } yet. Give me a couple of days and I will give
} a
} } report back.
} } }
} } } As for breaking the bank: Nikon D-50 body
} $720,
} } Capture software
} } } (needed for tethered operation) $100, and right
} } angle viewfinder
} } } $185.
} } }
} } } Right angle view finder: Very VERY helpful for
} } upright microscopes,
} } } but may not be needed / useful if you can use
} the
} } front camera port on
} } } an inverted scope.
} } }
}
=== message truncated ===

____________________________________________________
Start your day with Yahoo! - make it your home page
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==============================Original Headers==============================
6, 19 -- From hoffpajo-at-yahoo.com Wed Aug 17 12:06:16 2005
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6, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 19 -- Subject: Re: [Microscopy] viaWWW: LM Digital Camera Recommendations?
6, 19 -- To: Mike.Bode-at-soft-imaging.net, microscopy-at-microscopy.com
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yes by all means a side by side comarison is the way
to go.
i don't belive you can have to much hard disk or RAM,
or pixels. i typicaly enlarge and pics i take by quite
a bit. i use the nikon on a telescope for taking
images of the moon and other objects in daylight.
it is a hobby of mine.
john hoffpauir
adding my name here so you wont have to look for it in
the future.

--- wesaia-at-iastate.edu wrote:

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}
} Supposing the resolution is 200 nm, that is less
} than half a wavelength of
} light. That might be attainable given a lens (and
} other optics) of
} sufficiently high NA. Those are generally the higher
} power objectives. My
} basic 40x lens has an NA of only 0.63, so I won't
} get such good resolution.
} But suppose we could get 200 nm out of a 100x
} objective.
}
} 200 nm resolution means we would need pixels every
} 100 nm or so. I
} generally figure prints of 120 mm or so wide. That
} means the width of my
} image will be about 120 um at 1000x. That means I
} will need 1200 pixels
} across my image for a 100-nm pixel spacing. That
} isn't very many pixels in
} today's terms. My old 1.3 megapixel Pixera can
} handle that.
}
} You might say that the extra pixels allow you to
} take lower magnification
} images and still record up to the resolution limit.
} First, what is the
} resolution for that set of lenses? The NA and
} resolution falls off with a
} drop in magnification. There may not be detail to
} record with the extra
} pixels.
}
} Having said all that, 3 megapixel consumer cameras
} are practically throw
} away items now. For that matter, so are 40 GB disk
} drives. It doesn't hurt
} to add a few more pixels (and MB) to be safe.
} However, I think a lot of
} people are quick to take high-pixel images for which
} they never will use
} the information contained there. They just provide a
} demand for more and
} bigger hard drives.
}
} I am going to have to run a comparison here one of
} these days. I have a gut
} feeling that lower pixel number cameras may be more
} sensitive in low-light
} situations than their higher pixel cousins. (I think
} that is what Mike Bode
} suggested.) I want to do a side-by-side comparison
} to show myself and
} others. I will grant that higher pixel cameras can
} record more detail in
} their regular photographic mode under sufficient
} light. I just don't know
} that they have the same edge in the lab.
}
} Warren Straszheim
} (adding my name here so you don't have to check out
} the address line above
} or below)
}
} At 11:31 AM 08/17/05, John H. wrote:
}
} } again unless i am out of it isn't the highest
} } resolution possible to be about 200nm in a light
} } microscope? and pixel resolution to be between 3 to
} } 8um, i could be wrong. again very simplistic. so i
} } guess i should hang onto my D-70.
} } --- Mike.Bode-at-soft-imaging.net wrote:
} }
} } }
} } }
} } }
} } }
}
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }
}
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
}
} ----------------------------------------------------------------------------
} } }
} } } I think this post is too simplistic. Don't play
} the
} } } Pixel game when you buy a scientific camera. For
} } } example: If you buy an 8 Mpixel camera to work
} at
} } } high magnification on your microscope, you are
} } } wasting money and storage. The resolution will
} be
} } } limited by the microscope, and not by the number
} of
} } } pixels of the camera. Likewise, if you are doing
} } } Fluorescence, a camera with fewer pixels can be
} more
} } } sensitive and thus be superior to a camera with
} gobs
} } } of pixels.
} } }
} } } Also, please explain your comment that "all
} cameras
} } } ""see"" things as an 18% grey card".
} } }
} } } mike
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 12596 West Bayaud Avenue
} } } Suite 300
} } } Lakewood, CO 80228
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================
} } }
} } }
} } } -----Original Message-----
} } } X-from: hoffpajo-at-yahoo.com
} } } [mailto:hoffpajo-at-yahoo.com]
} } } Sent: Wednesday, August 17, 2005 9:28 AM
} } } To: Mike Bode
} } } Subject: [Microscopy] viaWWW: LM Digital Camera
} } } Recommendations?
} } }
} } }
} } }
} } }
} } }
}
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America To
} } } Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }
}
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
}
} ----------------------------------------------------------------------------
} } }
} } }
} } } for high quality images everything depends on
} the
} } } pixels. the more pixels the better the image.
} } } as for exposeures, everyone needs to remember
} all
} } } cameras ""see"" things as an 18% grey card. so
} all
} } } images need to be adjusted.
} } } and yes to all those of you about to jump on me
} i
} } } know this is simplistic.
} } } --- edelmare-at-muohio.edu wrote:
} } }
} } } }
} } } }
} } } }
} } } }
} } }
}
} ----------------------------------------------------------------------------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of
} } } } America To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } } }
} } }
}
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } }
}
} ----------------------------------------------------------------------------
} } } }
} } } } For years we have been using a Kodak
} DCS760
} } } (actually a Nikon F5 SLR
} } } } body with Kodak CDD and electronics).
} } } } The SLR's
} } } } are very nice because (1) they mount on the
} photo
} } } ports correctly
} } } } (i.e. F-mount, and otehrs), (2) they use the
} } } microscopes optics alone,
}
=== message truncated ===

____________________________________________________
Start your day with Yahoo! - make it your home page
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What you said here certainly makes sense in many cases. Other people have different requirements. For example, they might want to have a live image on a PC screen to do more processing, or integrate the camera into an automated system, or provide the images live over the internet to a colleague. Many other applications come to mind that require a different camera than the one you are suggesting.

This only supports my initial point that the number of pixels should not be the only parameter to look at when you buy a camera for a microscope.

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:� (888) FIND SIS
�� (303) 234-9270
fax:�� (303) 234-9271
email:� mailto:info-at-soft-imaging.com
web:�� http://www.soft-imaging.com
===================================

-----Original Message-----
X-from: john hoffpauir [mailto:hoffpajo-at-yahoo.com]
Sent: Wednesday, August 17, 2005 11:06 AM
To: Mike Bode; microscopy-at-microscopy.com

To: electron microprobe users, technicians, and lab managers

Re: new JEOL microprobe user listserver

Colleagues,

Rather than merely wishing for the twentieth time that there was a
listserver for JEOL microprobe users, technicians, and lab managers
to share questions, knowledge, news, problems, and experience (and
even socialize a bit), I decided to actually set up one.

There has been a listserver for Cameca SX50 users since 1996 to
discuss problems, suggest improvements to Cameca, plan meetings at
national conferences, and so on. I initially considered having a
8800/8900-only listserver, but I decided that there are more
similarities than differences across the generations. Whether you
use a classic JEOL JXA-733, a new field-emission 8500, or anything in-
between, this is for you. Of course, Cameca/ARL/whatever users are
welcome too!

I know, I know -- the last thing you need is more e-mail, but a
listserver is only as strong as its subscribers. Let's make this a
valuable resource! This, of course, is not meant to replace the
excellent MSA listserver (Thanks, Nestor!), but I think that it will
be valuable to more targeted list at our disposal, rather than e-
mailing all 3000 MSA subscribers. The same goes for the MAS
listserver too.

This is a moderated listserver, meaning all subscribers and all posts
are approved by a moderator (me). Moderation is intented to
eliminate spam and other junk mail from being sent to all subscribers.

There is a webpage with details here: http://probelab.geo.umn.edu/
listserver.html.

To subscribe, e-mail the message "SUB PROBEUSERS {YOUR_NAME} " to the
address: listserv-at-lists.umn.edu.

Please e-mail me with any subscription problems -- I want everyone to
be able to participate! Also please forward this to colleagues who
might be interested and forgive any multiple copies -- I'm trying to
announce this widely.

Best,
Ellery

--------------------
Ellery E. Frahm
Research Scientist/Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu

==============================Original Headers==============================
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Hi all,
In the same vein as the LM digital discussion my department was to
equip all the LM scopes in the introductory botany class with digital
cameras. They also want to be able to project what is being viewed on
the instructor's scope and have the students to be able to project what
is being viewed on their scopes (to share with the whole class).
Has anyone done this set up for their department? Or, know if there is
a company out there that would set up the lab for us?
Any help/suggestions would be greatly appreciated...especially from
vendors.
thanks,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***

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I would like to add a few statements to the discussion and support some
of the statements that Nestor made with his posting.

We had a demonstration at the M&M 2005 in our booth where we dipped
various samples into mechanical pump oil (hydrocarbon based), wiped them
with a cloth, estimated the contact angle, plasma cleaned them with our
PC2000 using just atmospheric air for 5 min, and then took them out, and
the samples were ultrahydrophilic (contact angle less than 5 degrees).
Of course prior to doing this at the show, we did it in our lab. We did
this demonstration to put a stake through the heart of any arguments
concerning an oil vs an oiless pumping system. What we did not make
public at the show were results from our studies that showed that using
Ar on the samples also had a significant cleaning affect. (We also did
not show the results for Fomblin(R) and silicone based oils that showed
the same results as the hydrocarbon oil.) Five to ten minutes with
about the same power as was used for air gave clean surfaces. These are
the similar parameters that Nestor is discussing below in his posting
and our results are in complete agreement with his findings. We did
find a difference with different materials, (semiconductor, ceramic, and
metal), but if cleaned long enough (maximum about 10 minutes) all of the
samples exhibited the cleaned surface void of hydrocarbons as evidenced
by the contact angle.

I also have anecdotal information concerning plasma cleaning of glass
TEM samples that were coated with carbon. I had samples that I ion
sputtered graphite and other samples that I evaporated carbon onto.
These samples were then plasma cleaned (in a competitor's plasma
cleaner). It has been awhile since I did this, but one form (I think
that it was the evaporated carbon) resisted plasma cleaning while carbon
from the ion sputtered samples was removed. I went almost 20 minutes
without the carbon being removed. This strongly suggests that the form
of carbon is very important as Nestor suggests. To my knowledge, nobody
has looked into this. I know that the amount of hydrogen in
diamond-like carbon (DLC) films does change the Raman spectra from these
films. Raman or FTIR techniques might be interesting method to use in
an attempt to relate to how different forms of carbon perform using
oxygen plasmas. I know that DLC films with differing levels of hydrogen
that are grown for tribological applications have different properties
in a humid environments. The more hydrogen in the films, the more they
are susceptible to failure in a humid atmosphere.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov]
Sent: Wednesday, August 17, 2005 9:06 AM
To: Walck-at-SouthBayTech.com

Roy

We routinely plasma clean holey/continuous carbon films with
particles on them it works
fine here at ANL. I've only done a few (i.e. { 5) microtomed
samples, but have not had
any problems with them, but that is not a significant number of
samples to gauge the
effectiveness.

The key here is to recognize, as you have apparently already done, that
the reactive plasma obviously will attack the carbon in your support as
well as the source of hydrocarbon contamination. I have found that the
hydrocarbon, not surprizingly is more reactive than, for
example, the more
stable carbon support films. The key here is to tailor your gas
composition
as well as to adjust the power levels of your plasma. At ANL, for
carbon support films, we
use Argon gas only and at a low power setting of ~ 5-10 W for ~ 10
minutes in our SBT PC-2000 plasma cleaner. In my experience, for these
type of samples, you should keep oxygen to a minimum as this will
rapidly attach all
carbon. Basically,
we have found that there will be enough residual oxygen released by
the Argon plasma
to attack the organic contamination, assuming the specimen is not
heavily coated.

Of course, not all "carbon support films", are created equal and
some have more
hydrocarbon than others, so you will need to test the receipe for your
films as well as for the model of plasma cleaner and its settings as
each manufactureres unit will vary in efficacy. It is best here to make
a few sacrifical films and tune your settings to minimze the reaction
on your support films.

Nestor
Your Friendly Neighborhood SysOp

Disclaimer: My employer Argonne National Lab holds the basic patent
on TEM/SEM plasma
cleaning technology and licenses this to manufacturers of commerical
units.

} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America

} Our specific samples are inorganic (mineral plus some carbonaceous
} material) grains in epoxy ultramicrotomy sections suppoted on
} continuous carbon film TEM grids. We would like to know whether
} contamination can be successfully prevented, or removed, from these
} samples by plasma cleaning (while in the TEM holder) without also
} causing destruction, alteration or volatilization of the epoxy section
} and/or carbon support film.
}
}
}
} Thanks,
}
}
}
} Roy Christoffersen
}
} SAIC
}
} NASA Johnson Space Center EM Facilities
}

--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================

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Hi Beth,

If you have a setup that allows sharing of a LCD projector between different PCs, all you would need is a camera that provides a live image on the PC screen. Student's PCs as well as the instructor's PC are connected to the projector. You would then simply switch between the PC to show what is on their screen.

Before you buy all that equipment, make sure that the quality of the projected image is sufficient. LCD projectors are very good for presentations, but the nuances of microscopic images might get lost.

Alternately you could use something like "remote desktop" to connect the instructor's PC to another PC in the classroom. The instructor's PC would then show the students desktop and be displayed via the projector. That way you would not have to deal with multiple connections to the projector, but the setup may be more complicated. Again, check the quality first. "remote desktop" will reduce colors if the bandwidth is too low, and you will get a bad image displayed on the screen.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:� (888) FIND SIS
�� (303) 234-9270
fax:�� (303) 234-9271
email:� mailto:info-at-soft-imaging.com
web:�� http://www.soft-imaging.com
===================================

-----Original Message-----
X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu]
Sent: Wednesday, August 17, 2005 1:13 PM
To: Mike Bode

Hi all,
In the same vein as the LM digital discussion my department was to equip all the LM scopes in the introductory botany class with digital cameras. They also want to be able to project what is being viewed on the instructor's scope and have the students to be able to project what is being viewed on their scopes (to share with the whole class).
Has anyone done this set up for their department? Or, know if there is a company out there that would set up the lab for us?
Any help/suggestions would be greatly appreciated...especially from vendors.
thanks,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805 http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***

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} I am going to have to run a comparison here one of these
} days. I have a gut
} feeling that lower pixel number cameras may be more sensitive
} in low-light
} situations than their higher pixel cousins. (I think that is
} what Mike Bode
} suggested.)

Lower pixel number means lower noise at low signal level.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jhjia-at-uwm.edu) from http://www.microscopy.com/MLFormMail.html on Wednesday, August 17, 2005 at 09:28:04
---------------------------------------------------------------------------

Email: jhjia-at-uwm.edu
Name: Junhong JIA

Organization: University of Wisconsin- Milwaukee

Title-Subject: [Filtered] MListserver:Ulruamicrotomy of carbon nanotubes in Poly(vinyl alcohol)

Question: Dear Sirs:

Is there any fluid that can be used in a knife boat for cutting utrathin sections from a specimen consitins of carbon nanotubes in poly(vinyl alcohol)?

Any helpful inforation should be highly appriciated.

Dr. Junhong JIA
Department of Mechanical Engineering
University of Wisconsin- Milwaukee
Milwaukee, WI 53211
Phone:414-229-2498

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Email: mkomboli-at-uno.edu
Name: Mary Kombolias

Organization: University of New Orleans

Title-Subject: [Filtered] LR gold

Question: Thanks to everybody who replied to my question! I appreciate the help. Peace out!

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There is more that I would like to add concerning plasma cleaners
affecting carbon films.

In the paper, "Surface Science Aspects of Contamination in TEM Sample
Preparation, J. T. Grant, S. D. Walck, F. J. Scheltens, A. A. Voevodin,
Proceedings of the Materials Research Society, Workshop on Specimen
Preparation for Transmission Electron Microscopy of Materials IV, eds.
Ron M. Anderson and Scott D. Walck, Pittsburgh, Vol 480, (1997), there
was a difference in the ability of the two types of plasma cleaners,
capacitively coupled and inductively coupled in the removal rate of
existing electron beam induced contamination. At the time, I thought
that this could be due to the difference in plasma densities between the
two systems. However, subsequent experiments at SBT showed that
pre-existing contamination could be removed by increasing the power and
lowering the pressure in the capacitively coupled system. (BTW, that's
SBT's design.) What these parameter changes do is increase the energies
of species in the plasma. In retrospect, the inductively coupled system
operated at a lower pressure than the capacitively coupled system and I
now believe that it is a difference in the energies of the species in
the plasma between the two systems that caused the difference. With
respect to the current topic question of how a plasma cleaner will
affect a carbon containing film, these parameters give another degree of
operator control in how the carbon containing materials are affected.

Incidentally, a recent flyer from a vender at the M&M 2005 meeting
claimed that an addition of hydrogen to the O2 plasma worked better and
that this was a discovery of theirs. It is well known in the literature
that an addition of H2 to a N2 or an O2 plasma will increase the
activated species of nitrogen and oxygen and in the case of oxygen are
the ones that are responsible for the cleaning. In fact, additions of
He will also increase the activated species and you don't have the
potential hazard. This is used in reactive sputter deposition systems
to increase the stoichiometry of oxide and nitride films. However, a
trade-off is that a light-element only plasma will increase the ion
temperature which could possible have adverse affects. The addition of
Ar which has a higher mass helps cool the plasma temperature. However,
keeping the results of Nestor's temperature rise experiments in mind,
I'm not sure how important this affect could be, but for
carbon-containing films, it could be very important.

I probably should give a disclaimer to the effect that SBT makes and
sells a plasma cleaner, but the above paragraphs are too "sciencey" as
opposed to "salesy", so I won't! OOPs, I guess I just did.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: rcsaic-at-sbcglobal.net [mailto:rcsaic-at-sbcglobal.net]
Sent: Wednesday, August 17, 2005 7:47 AM
To: Walck-at-SouthBayTech.com

We are interested in feedback from any group in the community who has
experience with plasma cleaning TEM samples prepared by ultramicrotomy.
Our specific samples are inorganic (mineral plus some carbonaceous
material) grains in epoxy ultramicrotomy sections suppoted on continuous
carbon film TEM grids. We would like to know whether contamination can
be successfully prevented, or removed, from these samples by plasma
cleaning (while in the TEM holder) without also causing destruction,
alteration or volatilization of the epoxy section and/or carbon support
film.

Thanks,

Roy Christoffersen

SAIC

NASA Johnson Space Center EM Facilities

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I could do with some help with my risk assessment. This stuff seems to
be fascinatingly reactive yet I know many labs use it for storing
biological samples. I am planning to store samples in 0.02% sodium
azide and sodium cacodylate for 3-4 months. On contact with water
sodium azide produces a toxic gas. One safety site said it should
therefore only be stored in a fume hood. Another site stated that it
should not be stored in fridges with exposed copper or lead parts. My
sparkproof fridge suppliers advised against it. I am dubious about
storing biological samples at room temperature. Any suggestions?

Dave

This email has been independently scanned for viruses and any virus software has been removed using McAfee anti-virus software

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I have been following this discussion on pixel number and resolution
for LM cameras with great interest.

It occurs to me that it might be useful to have a discussion of bit
depth as well. I realize that most cameras are standardized at 24
bits, which is really 8 bits per color channel. Although this does
give quite a range of color values, the intensity range is limited to
256 gray steps. I wonder if there is a need for greater range, so
that we can make finer disctinctions among intensities and/or record
a greater range, from bright to dim.

Joel

}
}
}
} ----------------------------------------------------------------------
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}
} } I am going to have to run a comparison here one of these
} } days. I have a gut
} } feeling that lower pixel number cameras may be more sensitive
} } in low-light
} } situations than their higher pixel cousins. (I think that is
} } what Mike Bode
} } suggested.)
}
} Lower pixel number means lower noise at low signal level.
}
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
} ==============================Original
} Headers============================== 6, 23 -- From DusevichV-at-umkc.edu
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} Recommendations? 6, 23 -- Date: Wed, 17 Aug 2005 17:27:11 -0500 6, 23
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Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs

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I highly recommend http://www.photomet.com/library_encyclopedia.shtml

At 11:08 AM 08/18/05 -0500, you wrote:

} I have been following this discussion on pixel number and resolution
} for LM cameras with great interest.
}
} It occurs to me that it might be useful to have a discussion of bit
} depth as well. I realize that most cameras are standardized at 24
} bits, which is really 8 bits per color channel. Although this does
} give quite a range of color values, the intensity range is limited to
} 256 gray steps. I wonder if there is a need for greater range, so
} that we can make finer disctinctions among intensities and/or record
} a greater range, from bright to dim.
}
} Joel

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**

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7, 24 -- Subject: Re: [Microscopy] Re: viaWWW: LM Digital Camera
7, 24 -- Recommendations?
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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Postdoctoral Position in Scanning Transmission Electron Microscopy -
University of California, Santa Barbara

Applicants must have extensive and demonstrated experience in several areas
of TEM and a strong background in materials science and diffraction.
Preference will be given to applicants with expertise in STEM techniques,
such as atomic resolution HAADF imaging. Facilities at UCSB include a
Tecnai F30U TEM/STEM and other state-of-the-art imaging, spectroscopy and
diffraction facilities in the UCSB MRL. Research projects include the
characterization of high-permittivity oxide thin films, including gate
dielectrics and ferroelectrics.

The position is available in early 2006. Duration about 1-2 years, salary
is commensurate with
qualifications. Candidates must have a Ph.D. in Materials Science or
Physics. Interested candidates should send a curriculum vitae, publication
list and the names of at least three references with their contact
information to:

Prof. Susanne Stemmer
Materials Department
University of California
Santa Barbara, CA 93106-5050
Email: stemmer-at-mrl.ucsb.edu
http://www.mrl.ucsb.edu/~stemmer
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

==============================Original Headers==============================
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5, 24 -- Subject: Postdoctoral Position in Scanning Transmission Electron
5, 24 -- Microscopy - University of California, Santa Barbara
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Thanks Michael. It is a useful resource.

It seems to the me that the real determinant of how much need there
is for increased bit depth is a function of the images. Many of the
fluorescence images I have seen appear to be saturated, in which case
perhaps 2 or four bits would be sufficient. However, if one is
interested in FRET or other quantitative techniques, the bit depth
may matter. As with pixel density, bit depth depends on the samples.

} I highly recommend http://www.photomet.com/library_encyclopedia.shtml
}
}
}
}
} At 11:08 AM 08/18/05 -0500, you wrote:
}
}
}
} } ---------------------------------------------------------------------
} } ------- The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ---------------------------------------------------------------------
} } -------
} }
} } I have been following this discussion on pixel number and resolution
} } for LM cameras with great interest.
} }
} } It occurs to me that it might be useful to have a discussion of bit
} } depth as well. I realize that most cameras are standardized at 24
} } bits, which is really 8 bits per color channel. Although this does
} } give quite a range of color values, the intensity range is limited to
} } 256 gray steps. I wonder if there is a need for greater range, so
} } that we can make finer disctinctions among intensities and/or record
} } a greater range, from bright to dim.
} }
} } Joel
} }
} }
} } }
} } }
} } }
} } } ------------------------------------------------------------------
} } } ---- ------ The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ------------------------------------------------------------------
} } } ---- ------
} } }
} } } } I am going to have to run a comparison here one of these
} } } } days. I have a gut
} } } } feeling that lower pixel number cameras may be more sensitive in
} } } } low-light situations than their higher pixel cousins. (I think
} } } } that is what Mike Bode suggested.)
} } }
} } } Lower pixel number means lower noise at low signal level.
} } }
} } }
} } } Vladimir M. Dusevich, Ph.D.
} } } Electron Microscope Lab Manager
} } } 3127 School of Dentistry
} } } 650 E. 25th Street
} } } Kansas City, MO 64108-2784
} } }
} } } Phone: (816) 235-2072
} } } Fax: (816) 235-5524
} } } Web: http://www.umkc.edu/dentistry/microscopy
} } }
} } }
} } } ==============================Original
} } } Headers============================== 6, 23 -- From
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} } } from kc-msxproto3.kc.umkc.edu (imap4.exchange.umkc.edu
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} } } Subject: RE: [Microscopy] Re: viaWWW: LM Digital Camera
} } } Recommendations? 6, 23 -- Date: Wed, 17 Aug 2005 17:27:11 -0500 6,
} } } 23 -- Message-ID:
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} } } Recommendations? 6, 23 -- Thread-Index:
} } } AcWjTe7nuT+e71apTjeoVuPp2YSpdQALDjBQ 6, 23 -- From: "Dusevich,
} } } Vladimir" {DusevichV-at-umkc.edu} 6, 23 -- To:
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} }
} }
} } Joel B. Sheffield, Ph.D.
} } Biology Department, Temple University
} } 1900 North 12th Street
} } Philadelphia, PA 19122
} } jbs-at-temple.edu
} } (215) 204 8839, fax (215) 204 0486
} } http://astro.temple.edu/~jbs
} }
} }
} } ==============================Original
} } Headers============================== 8, 20 -- From jbs-at-temple.edu
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} } 12:05:09 -0400 8, 20 -- From: "Joel Sheffield" {jbs-at-temple.edu} 8, 20
} } -- To: microscopy-at-microscopy.com 8, 20 -- Date: Thu, 18 Aug 2005
} } 12:05:08 -0400 8, 20 -- MIME-Version: 1.0 8, 20 -- Subject: Re:
} } [Microscopy] viaWWW: LM Digital Camera Recommendations? 8, 20 --
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}
} ______________________________________________________________________
} ______ Michael Cammer Analytical Imaging Facility Albert Einstein
} Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave.
} Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains information that is
} privileged.**
}

Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs

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You are absolutely right that even a 24-bit image can only show 256 levels of gray. This compares to about 60 or so that the human eye can distinguish under optimum circumstances. Also, as far as I know, there are no monitors out there that can display more than 8 bit per color, so for displaying the images, this range is probably sufficient.

However, most people want to do further processing of the images, or they need a larger range for acquiring the images (diffraction patterns come to mind), and in fact, most cameras can provide images that have a wider range. Just as an example, our Cantega (a TEM b/w camera), can provide 16 bit of gray level information, or 65K gray levels. These images can then be stored as 16-bit gray level images to keep all the information contained in the image. The question then turns to how you can display this on an 8-bit monitor, but there are solutions to that. Other cameras have 14 , 12 or 10 bit ranges, and they are typically also stored as 16-bit files. For color, there is a 48-bit format that can be used. The files are getting pretty large then. If you have a 5 Mpixel color camera and store the image as a 48-bit file, each image requires 30 MB of storage.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:� (888) FIND SIS
�� (303) 234-9270
fax:�� (303) 234-9271
email:� mailto:info-at-soft-imaging.com
web:�� http://www.soft-imaging.com
===================================

-----Original Message-----
X-from: cammer-at-aecom.yu.edu [mailto:cammer-at-aecom.yu.edu]
Sent: Thursday, August 18, 2005 10:17 AM
To: Mike Bode

I highly recommend http://www.photomet.com/library_encyclopedia.shtml

At 11:08 AM 08/18/05 -0500, you wrote:

} I have been following this discussion on pixel number and resolution
} for LM cameras with great interest.
}
} It occurs to me that it might be useful to have a discussion of bit
} depth as well. I realize that most cameras are standardized at 24
} bits, which is really 8 bits per color channel. Although this does
} give quite a range of color values, the intensity range is limited to
} 256 gray steps. I wonder if there is a need for greater range, so that
} we can make finer disctinctions among intensities and/or record a
} greater range, from bright to dim.
}
} Joel

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**

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7, 24 -- Recommendations?
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Postdoctoral Position in Scanning Transmission Electron Microscopy -
University of California, Santa Barbara

Applicants must have extensive and demonstrated experience in several areas
of TEM and a strong background in materials science and diffraction.
Preference will be given to applicants with expertise in STEM techniques,
such as atomic resolution HAADF imaging. Facilities at UCSB include a
Tecnai F30U TEM/STEM and other state-of-the-art imaging, spectroscopy and
diffraction facilities in the UCSB MRL. Research projects include the
characterization of high-permittivity oxide thin films, including gate
dielectrics and ferroelectrics.

The position is available in early 2006. Duration about 1-2 years, salary
is commensurate with
qualifications. Candidates must have a Ph.D. in Materials Science or
Physics. Interested candidates should send a curriculum vitae, publication
list and the names of at least three references with their contact
information to:

Prof. Susanne Stemmer
Materials Department
University of California
Santa Barbara, CA 93106-5050
Email: stemmer-at-mrl.ucsb.edu
http://www.mrl.ucsb.edu/~stemmer

==============================Original Headers==============================
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5, 24 -- Subject: Postdoctoral Position in Scanning Transmission Electron
5, 24 -- Microscopy - University of California, Santa Barbara
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, August 18, 2005 at 09:57:24
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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: I have been catching up on some lab cleaning and have about 12 gallons of spent photographic fixer. I have been told that I can sink dispose of this using copious amounts of water or pay to have our chemical disposal contractor pick it up and dispose of it. Does anyone have a simple on-site silver recovery system I could try first? Is it worth the trouble/expense?
Thanks for any help.

Stacey Andringa

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Mike,

As you imply, the higher bit depth in B&W as well as color might be useful
performing image analysis where display of the image is not necessary but
obtaining data and statistics is. Re file size, the Nikon D2X raw image
opened in Image Capture and then converted to a TIFF file in Photoshop is
70MB! Takes a bit of processing power, that one does; think I'm going to
have to go to a dual processor system.

Damian Neuberger, Ph.D.

You are absolutely right that even a 24-bit image can only show 256 levels
of gray. This compares to about 60 or so that the human eye can distinguish
under optimum circumstances. Also, as far as I know, there are no monitors
out there that can display more than 8 bit per color, so for displaying the
images, this range is probably sufficient.

However, most people want to do further processing of the images, or they
need a larger range for acquiring the images (diffraction patterns come to
mind), and in fact, most cameras can provide images that have a wider range.
Just as an example, our Cantega (a TEM b/w camera), can provide 16 bit of
gray level information, or 65K gray levels. These images can then be stored
as 16-bit gray level images to keep all the information contained in the
image. The question then turns to how you can display this on an 8-bit
monitor, but there are solutions to that. Other cameras have 14 , 12 or 10
bit ranges, and they are typically also stored as 16-bit files. For color,
there is a 48-bit format that can be used. The files are getting pretty
large then. If you have a 5 Mpixel color camera and store the image as a
48-bit file, each image requires 30 MB of storage.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228

==============================Original Headers==============================
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jroszka-at-beaumont.edu) from http://www.microscopy.com/MLFormMail.html on Friday, August 19, 2005 at 07:20:30
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Email: jroszka-at-beaumont.edu
Name: jroszka

Organization: w.beaumonthospital

Title-Subject: [Filtered] MListserver:

Question: Looking into the DITABIS Imaging Plate Technology for TEM photography. Anyone with practical expierence or insight with this system (this system would be applied to a Philips 201 TEM) ? Thanks

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Listers,

We're considering a tissue processor for EM samples. We would like
to hear from any of you that have thoughts about the Leica EM TP
Tissue Processor or the EMS LYNX Tissue Processor. The usual types
of questions like do they work well, how is your support, price, etc.
or any other information that you may think is important would be
very useful. Thank you.

Dotty Sorenson
Microscopy and Image-analysis Laboratory
University of Michigan

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Here in New Jersey we are required to dispose of used fixer as hazardous
waste, due to the silver content. Kodak sells (or used to sell) a silver
recovery kit. so you might try them.

Geoff

Stacey.Andringa-at-uc.edu wrote:

}
} ----------------------------------------------------------------------------
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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
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i remember those days when i worked in NJ, it was a
pain in the but to collect all the spent fixer. you
are right about kodax and the silver recovery kit.
someone else used to do it as well but alas that brain
cell is long gone.
i do believe if you truly just want the silver in it's
raw form you can use steel wool so the silver will
react with the steel wool and bind to it. perhaps some
on the list server can explain the chemistry to us
all. i was never any good at inorganic chemisrty.
john hoffpauir

--- mcauliff-at-umdnj.edu wrote:

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} Here in New Jersey we are required to dispose of
} used fixer as hazardous
} waste, due to the silver content. Kodak sells (or
} used to sell) a silver
} recovery kit. so you might try them.
}
} Geoff
}
} Stacey.Andringa-at-uc.edu wrote:
}
} }
}
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} (NJZFM-ultra-55). It was submitted by
} (Stacey.Andringa-at-uc.edu) from
} http://www.microscopy.com/MLFormMail.html on
} Thursday, August 18, 2005 at 09:57:24
}
} ---------------------------------------------------------------------------
} }
} } Email: Stacey.Andringa-at-uc.edu
} } Name: Stacey Andringa
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:
} }
} } Question: I have been catching up on some lab
} cleaning and have about 12 gallons of spent
} photographic fixer. I have been told that I can sink
} dispose of this using copious amounts of water or
} pay to have our chemical disposal contractor pick it
} up and dispose of it. Does anyone have a simple
} on-site silver recovery system I could try first? Is
} it worth the trouble/expense?
} } Thanks for any help.
} }
} } Stacey Andringa
} }
} }
}
} ---------------------------------------------------------------------------
} }
} } ==============================Original
} Headers==============================
} } 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 18
} 17:28:23 2005
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} }
} }
} }
}
}
} --
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************
}
}
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} 8, 32 -- Date: Fri, 19 Aug 2005 13:00:14 -0400
} 8, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
} 8, 32 -- Subject: Re: [Microscopy] viaWWW: spent
} photographic fixer
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7, 19 -- From hoffpajo-at-yahoo.com Fri Aug 19 14:53:14 2005
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (winston.wiggins-at-cshs.org) from http://www.microscopy.com/MLFormMail.html on Friday, August 19, 2005 at 12:29:21
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Email: winston.wiggins-at-cshs.org
Name: Winston Wiggins

Organization: Cedars-Sinai Medical Center

Title-Subject: [Filtered] MListserver: spent fixer

Question: Stacey,
My understanding is that unused fixer can be sent down the drain with copious amounts of water but not spent fixer because of the silver content. I am hesitant to put anything down the drain other than water, so I've always used a silver-recovery system service.
If UC has a a Radiology Dept in its system, check with their mode of disposal or try a commercial photo lab to see what they do. They may even take it off your hands for disposal. I'd check the local regs and/or Haz Waste Dept before I put anything down the drain. It invariably comes back in your drinking water, IMO that is.
Winston Wiggins

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (LDUH-at-SIKORSKY.COM) from http://www.microscopy.com/MLFormMail.html on Friday, August 19, 2005 at 12:43:05
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Email: LDUH-at-SIKORSKY.COM
Name: LOU DUH

Organization: MSA / SIKORSKY AIRCRAFT

Title-Subject: [Filtered] Fatigue appearance in C355-T6 aluminum alloy.:

Question: I am currently examining an aircraft part which is made of aluminum C355-T6. I am fairly accustomed to cast aluminum, but I do not have experience wiht the fatigue appearances of this paticular alloy. What I see appears to be elongated eutectic regions with some very faint crack front markings. The surface appears somewhat rubbed and is making identification of stiation marks very difficult. Does anyone know where I can find some information about this subject - prefferably with images?? Thank you very much.

Lou

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7, 12 -- From zaluzec-at-microscopy.com Fri Aug 19 17:49:55 2005
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Friday, August 19, 2005 at 14:43:45
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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: Thanks for all the responses about spent photographic fixer. There is a researcher here who does silver recovery and will dispose of the rest as hazardous waste.

Stacey

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7, 12 -- From zaluzec-at-microscopy.com Fri Aug 19 17:50:26 2005
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Hmmm.....There's a product called SilverMagnet SMT-20
that is compact and collects via electrolysis. And
there are also EPA certified steel wool buckets that
separate the silver out but those are mostly for low
concentration baths. Not for fixer based soln.s

Most large photo supply places will have some sort of
kit that will collect the silver that you can turn in
for a small check.

Check www.silvercouncil.org. They will direct you to
a suitable method for your use.

--- hoffpajo-at-yahoo.com wrote:

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} i remember those days when i worked in NJ, it was a
} pain in the but to collect all the spent fixer. you
} are right about kodax and the silver recovery kit.
} someone else used to do it as well but alas that
} brain
} cell is long gone.
} i do believe if you truly just want the silver in
} it's
} raw form you can use steel wool so the silver will
} react with the steel wool and bind to it. perhaps
} some
} on the list server can explain the chemistry to us
} all. i was never any good at inorganic chemisrty.
} john hoffpauir
}
}
}
} --- mcauliff-at-umdnj.edu wrote:
}
} }
} }
} }
} }
}
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} }
} } Here in New Jersey we are required to dispose of
} } used fixer as hazardous
} } waste, due to the silver content. Kodak sells (or
} } used to sell) a silver
} } recovery kit. so you might try them.
} }
} } Geoff
} }
} } Stacey.Andringa-at-uc.edu wrote:
} }
} } }
} }
}
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
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}
} ----------------------------------------------------------------------------
} } }
} } } Below is the result of your feedback form
} } (NJZFM-ultra-55). It was submitted by
} } (Stacey.Andringa-at-uc.edu) from
} } http://www.microscopy.com/MLFormMail.html on
} } Thursday, August 18, 2005 at 09:57:24
} }
}
} ---------------------------------------------------------------------------
} } }
} } } Email: Stacey.Andringa-at-uc.edu
} } } Name: Stacey Andringa
} } }
} } } Organization: University of Cincinnati
} } }
} } } Title-Subject: [Filtered] MListserver:
} } }
} } } Question: I have been catching up on some lab
} } cleaning and have about 12 gallons of spent
} } photographic fixer. I have been told that I can
} sink
} } dispose of this using copious amounts of water or
} } pay to have our chemical disposal contractor pick
} it
} } up and dispose of it. Does anyone have a simple
} } on-site silver recovery system I could try first?
} Is
} } it worth the trouble/expense?
} } } Thanks for any help.
} } }
} } } Stacey Andringa
} } }
} } }
} }
}
} ---------------------------------------------------------------------------
} } }
} } } ==============================Original
} } Headers==============================
} } } 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 18
} } 17:28:23 2005
} } } 8, 12 -- Received: from [206.69.208.22]
} } (mac22.zaluzec.com [206.69.208.22])
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} } with ESMTP id j7IMSLGo006890
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} } } 8, 12 -- To: microscopy-at-microscopy.com
} } } 8, 12 -- From: Stacey.Andringa-at-uc.edu (by way of
} } MicroscopyListserver)
} } } 8, 12 -- Subject: viaWWW: spent photographic
} fixer
} } } 8, 12 -- Content-Type: text/plain;
} } charset="us-ascii"
} } } ==============================End of -
} } Headers==============================
} } }
} } }
} } }
} }
} }
} } --
} } --
} } **********************************************
} } Geoff McAuliffe, Ph.D.
} } Neuroscience and Cell Biology
} } Robert Wood Johnson Medical School
} } 675 Hoes Lane, Piscataway, NJ 08854
} } voice: (732)-235-4583; fax: -4029
} } mcauliff-at-umdnj.edu
} } **********************************************
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 8, 32 -- From mcauliff-at-umdnj.edu Fri Aug 19
} 11:59:45
} } 2005
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} } (original mail from mcauliff-at-umdnj.edu)
} } 8, 32 -- for microscopy-at-msa.microscopy.com; Fri,
} 19
} } Aug 2005 12:59:42 -0400 (EDT)
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} } 8, 32 -- Date: Fri, 19 Aug 2005 13:00:14 -0400
} } 8, 32 -- From: Geoff McAuliffe
} {mcauliff-at-umdnj.edu}
} } 8, 32 -- Subject: Re: [Microscopy] viaWWW: spent
} } photographic fixer
} } 8, 32 -- In-reply-to:
} } {200508182230.j7IMULQ8009016-at-ns.microscopy.com}
} } 8, 32 -- To: Stacey.Andringa-at-uc.edu,
} } 8, 32 -- MicroscopyListserver
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}
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==============================Original Headers==============================
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6, 15 -- Date: Fri, 19 Aug 2005 15:57:30 -0700 (PDT)
6, 15 -- From: "r. williams" {s2kdude-at-pacbell.net}
6, 15 -- Subject: Re: [Microscopy] viaWWW: spent photographic fixer
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Contents Retrieved from Microscopy Listserver Archives
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Lou:

One good reference for fracture surfaces would be the
Metals Handbook, Ninth Edition, Volume 12,
"Fractography". Another is titled "How Components
Fail" by Donald Wulpi.

I have a lot of experience looking at fracture
surfaces both macro and micro (SEM) examination. I'd
be glad to comment on any images you may want send to
my business e-mail.

Stu Smalinskas, P.E.
Metallurgist
SKF
Plymouth, Michigan
(734) 414-6862
stu.smalinskas-at-skf.com

--- LDUH-at-SIKORSKY.COM wrote:

}
} Email: LDUH-at-SIKORSKY.COM
} Name: LOU DUH
}
} Organization: MSA / SIKORSKY AIRCRAFT
}
} Title-Subject: [Filtered] Fatigue appearance in
} C355-T6 aluminum alloy.:
}
} Question: I am currently examining an aircraft part
} which is made of aluminum C355-T6. I am fairly
} accustomed to cast aluminum, but I do not have
} experience with the fatigue appearances of this
} paticular alloy. What I see appears to be elongated
} eutectic regions with some very faint crack front
} markings. The surface appears somewhat rubbed and
} is making identification of stiation marks very
} difficult. Does anyone know where I can find some
} information about this subject - preferably with
} images?? Thank you very much.
}
} Lou
}

__________________________________________________
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==============================Original Headers==============================
8, 19 -- From smalinskas-at-yahoo.com Fri Aug 19 20:53:15 2005
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Contents Retrieved from Microscopy Listserver Archives
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Perhaps I can put this in perspective for you. Most
states release some raw sewage. It generally happens
by accident. Either a broken pipe or a malfuntion of
the waste plant, It is not just NJ. I am not an
apologist for NJ so how the flames please,

As for the NJ reg, I started work in NJ over 15 years
ago and it was a reg back then, not sure if that is a
generation or not. You tell me. Some states, I was
working in Dallas for a lot of years allowed fixer to
be dumped down the drain when I was working there.
Perhaps some one from Texas can tell me if this is
still alowed.

I should note that mercury is released into the water
constantly, it is a by product of coal plants
producing energy. How many of us recycle batteries or
just throw them in the trash and forget about them.
there is no state that requires we recycle them.

--- John Twilley {jtwilley-at-sprynet.com} wrote:

} According to a recent article in the NYT, New Jersey
} still has annual releases of raw sewage into the
} local waterways annually in the millions of gallons.
} So perhaps it has not been long that
} they have had restrictions. In most parts of the
} country dissolved metals haven't been permitted to
} be dumped down the drain in a generation.
}
} John
}
} hoffpajo-at-yahoo.com wrote:
}
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------------
} }
} } i remember those days when i worked in NJ, it was
} a
} } pain in the but to collect all the spent fixer.
} you
} } are right about kodax and the silver recovery kit.
} } someone else used to do it as well but alas that
} brain
} } cell is long gone.
} } i do believe if you truly just want the silver in
} it's
} } raw form you can use steel wool so the silver will
} } react with the steel wool and bind to it. perhaps
} some
} } on the list server can explain the chemistry to us
} } all. i was never any good at inorganic chemisrty.
} } john hoffpauir
} }
} } --- mcauliff-at-umdnj.edu wrote:
} }
} } }
} } }
} } }
} } }
} }
}
----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
}
----------------------------------------------------------------------------
} } }
} } } Here in New Jersey we are required to dispose of
} } } used fixer as hazardous
} } } waste, due to the silver content. Kodak sells
} (or
} } } used to sell) a silver
} } } recovery kit. so you might try them.
} } }
} } } Geoff
} } }
} } } Stacey.Andringa-at-uc.edu wrote:
} } }
} } } }
} } }
} }
}
} ----------------------------------------------------------------------------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
} } } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
}
} ----------------------------------------------------------------------------
} } } }
} } } } Below is the result of your feedback form
} } } (NJZFM-ultra-55). It was submitted by
} } } (Stacey.Andringa-at-uc.edu) from
} } } http://www.microscopy.com/MLFormMail.html on
} } } Thursday, August 18, 2005 at 09:57:24
} } }
} }
}
} ---------------------------------------------------------------------------
} } } }
} } } } Email: Stacey.Andringa-at-uc.edu
} } } } Name: Stacey Andringa
} } } }
} } } } Organization: University of Cincinnati
} } } }
} } } } Title-Subject: [Filtered] MListserver:
} } } }
} } } } Question: I have been catching up on some lab
} } } cleaning and have about 12 gallons of spent
} } } photographic fixer. I have been told that I can
} sink
} } } dispose of this using copious amounts of water
} or
} } } pay to have our chemical disposal contractor
} pick it
} } } up and dispose of it. Does anyone have a simple
} } } on-site silver recovery system I could try
} first? Is
} } } it worth the trouble/expense?
} } } } Thanks for any help.
} } } }
} } } } Stacey Andringa
} } } }
} } } }
} } }
} }
}
} ---------------------------------------------------------------------------
} } } }
} } } } ==============================Original
} } } Headers==============================
} } } } 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 18
} } } 17:28:23 2005
} } } } 8, 12 -- Received: from [206.69.208.22]
} } } (mac22.zaluzec.com [206.69.208.22])
} } } } 8, 12 -- by ns.microscopy.com
} (8.12.11/8.12.8)
} } } with ESMTP id j7IMSLGo006890
} } } } 8, 12 -- for {microscopy-at-microscopy.com} ;
} Thu, 18
} } } Aug 2005 17:28:22 -0500
} } } } 8, 12 -- Mime-Version: 1.0
} } } } 8, 12 -- X-Sender: (Unverified)
} } } } 8, 12 -- Message-Id:
} } } {p06110400bf2abbf3cfd8-at-[206.69.208.22]}
} } } } 8, 12 -- Date: Thu, 18 Aug 2005 17:28:21 -0500
} } } } 8, 12 -- To: microscopy-at-microscopy.com
} } } } 8, 12 -- From: Stacey.Andringa-at-uc.edu (by way
} of
} } } MicroscopyListserver)
} } } } 8, 12 -- Subject: viaWWW: spent photographic
} fixer
} } } } 8, 12 -- Content-Type: text/plain;
} } } charset="us-ascii"
} } } } ==============================End of -
} } } Headers==============================
} } } }
} } } }
} } } }
} } }
} } }
} } } --
} } } --
} } } **********************************************
} } } Geoff McAuliffe, Ph.D.
} } } Neuroscience and Cell Biology
} } } Robert Wood Johnson Medical School
} } } 675 Hoes Lane, Piscataway, NJ 08854
} } } voice: (732)-235-4583; fax: -4029
} } } mcauliff-at-umdnj.edu
} } } **********************************************
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 8, 32 -- From mcauliff-at-umdnj.edu Fri Aug 19
} 11:59:45
} } } 2005
} } } 8, 32 -- Received: from mail01.umdnj.edu
} } } (zix01.UMDNJ.EDU [130.219.34.124])
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} (8.12.11/8.12.8) with
} } } ESMTP id j7JGxjmx017595
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} } } 19 Aug 2005 11:59:45 -0500
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}
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____________________________________________________
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==============================Original Headers==============================
8, 19 -- From hoffpajo-at-yahoo.com Sat Aug 20 09:58:14 2005
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8, 19 -- Date: Sat, 20 Aug 2005 07:58:13 -0700 (PDT)
8, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
8, 19 -- Subject: Re: [Microscopy] viaWWW: spent photographic fixer
8, 19 -- To: John Twilley {jtwilley-at-sprynet.com} , microscopy-at-msa.microscopy.com
8, 19 -- In-Reply-To: {4306457D.7FCA4086-at-sprynet.com}
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Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

So your point is? "Just do it if you can get away with it" and can point to
something else that's worse?

I pointed out, off list, to the original inquirer that most jurisdictions limit
silver (in spent fixer) because it is a biocide that can cause a die-off of the
bacteria that are required for the normal functioning of waste water treatment
plants.

As an alternative to paying a waste hauler to remove fixer, one can sign up a
precious metal recovery company to remove it. Usually this is a break-even
proposition with the recovery firm providing a container and pickup service in
exchange for keeping the silver and handling the disposal of the silver-free
remains.

John

john hoffpauir wrote:

} Perhaps I can put this in perspective for you. Most
} states release some raw sewage. It generally happens
} by accident. Either a broken pipe or a malfuntion of
} the waste plant, It is not just NJ. I am not an
} apologist for NJ so how the flames please,
}
} As for the NJ reg, I started work in NJ over 15 years
} ago and it was a reg back then, not sure if that is a
} generation or not. You tell me. Some states, I was
} working in Dallas for a lot of years allowed fixer to
} be dumped down the drain when I was working there.
} Perhaps some one from Texas can tell me if this is
} still alowed.
}
} I should note that mercury is released into the water
} constantly, it is a by product of coal plants
} producing energy. How many of us recycle batteries or
} just throw them in the trash and forget about them.
} there is no state that requires we recycle them.
}
} --- John Twilley {jtwilley-at-sprynet.com} wrote:
}
} } According to a recent article in the NYT, New Jersey
} } still has annual releases of raw sewage into the
} } local waterways annually in the millions of gallons.
} } So perhaps it has not been long that
} } they have had restrictions. In most parts of the
} } country dissolved metals haven't been permitted to
} } be dumped down the drain in a generation.
} }
} } John
} }
} } hoffpajo-at-yahoo.com wrote:
} }
} } }
} }
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
} ----------------------------------------------------------------------------
} } }
} } } i remember those days when i worked in NJ, it was
} } a
} } } pain in the but to collect all the spent fixer.
} } you
} } } are right about kodax and the silver recovery kit.
} } } someone else used to do it as well but alas that
} } brain
} } } cell is long gone.
} } } i do believe if you truly just want the silver in
} } it's
} } } raw form you can use steel wool so the silver will
} } } react with the steel wool and bind to it. perhaps
} } some
} } } on the list server can explain the chemistry to us
} } } all. i was never any good at inorganic chemisrty.
} } } john hoffpauir
} } }
} } } --- mcauliff-at-umdnj.edu wrote:
} } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
} ----------------------------------------------------------------------------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } } Microscopy Society of America
} } } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } } }
} } }
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } }
} }
} ----------------------------------------------------------------------------
} } } }
} } } } Here in New Jersey we are required to dispose of
} } } } used fixer as hazardous
} } } } waste, due to the silver content. Kodak sells
} } (or
} } } } used to sell) a silver
} } } } recovery kit. so you might try them.
} } } }
} } } } Geoff
} } } }
} } } } Stacey.Andringa-at-uc.edu wrote:
} } } }
} } } } }
} } } }
} } }
} }
} } ----------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- CoSponsor: The
} } } } Microscopy Society of America
} } } } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } } On-Line Help
} } } }
} } }
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } }
} }
} } ----------------------------------------------------------------------------
} } } } }
} } } } } Below is the result of your feedback form
} } } } (NJZFM-ultra-55). It was submitted by
} } } } (Stacey.Andringa-at-uc.edu) from
} } } } http://www.microscopy.com/MLFormMail.html on
} } } } Thursday, August 18, 2005 at 09:57:24
} } } }
} } }
} }
} } ---------------------------------------------------------------------------
} } } } }
} } } } } Email: Stacey.Andringa-at-uc.edu
} } } } } Name: Stacey Andringa
} } } } }
} } } } } Organization: University of Cincinnati
} } } } }
} } } } } Title-Subject: [Filtered] MListserver:
} } } } }
} } } } } Question: I have been catching up on some lab
} } } } cleaning and have about 12 gallons of spent
} } } } photographic fixer. I have been told that I can
} } sink
} } } } dispose of this using copious amounts of water
} } or
} } } } pay to have our chemical disposal contractor
} } pick it
} } } } up and dispose of it. Does anyone have a simple
} } } } on-site silver recovery system I could try
} } first? Is
} } } } it worth the trouble/expense?
} } } } } Thanks for any help.
} } } } }
} } } } } Stacey Andringa
} } } } }
} } } } }
} } } }
} } }
} }
} } ---------------------------------------------------------------------------
} } } } }
} } } } } ==============================Original
} } } } Headers==============================
} } } } } 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 18
} } } } 17:28:23 2005
} } } } } 8, 12 -- Received: from [206.69.208.22]
} } } } (mac22.zaluzec.com [206.69.208.22])
} } } } } 8, 12 -- by ns.microscopy.com
} } (8.12.11/8.12.8)
} } } } with ESMTP id j7IMSLGo006890
} } } } } 8, 12 -- for {microscopy-at-microscopy.com} ;
} } Thu, 18
} } } } Aug 2005 17:28:22 -0500
} } } } } 8, 12 -- Mime-Version: 1.0
} } } } } 8, 12 -- X-Sender: (Unverified)
} } } } } 8, 12 -- Message-Id:
} } } } {p06110400bf2abbf3cfd8-at-[206.69.208.22]}
} } } } } 8, 12 -- Date: Thu, 18 Aug 2005 17:28:21 -0500
} } } } } 8, 12 -- To: microscopy-at-microscopy.com
} } } } } 8, 12 -- From: Stacey.Andringa-at-uc.edu (by way
} } of
} } } } MicroscopyListserver)
} } } } } 8, 12 -- Subject: viaWWW: spent photographic
} } fixer
} } } } } 8, 12 -- Content-Type: text/plain;
} } } } charset="us-ascii"
} } } } } ==============================End of -
} } } } Headers==============================
} } } } }
} } } } }
} } } } }
} } } }
} } } }
} } } } --
} } } } --
} } } } **********************************************
} } } } Geoff McAuliffe, Ph.D.
} } } } Neuroscience and Cell Biology
} } } } Robert Wood Johnson Medical School
} } } } 675 Hoes Lane, Piscataway, NJ 08854
} } } } voice: (732)-235-4583; fax: -4029
} } } } mcauliff-at-umdnj.edu
} } } } **********************************************
} } } }
} } } }
} } } }
} } } } ==============================Original
} } } } Headers==============================
} } } } 8, 32 -- From mcauliff-at-umdnj.edu Fri Aug 19
} } 11:59:45
} } } } 2005
} } } } 8, 32 -- Received: from mail01.umdnj.edu
} } } } (zix01.UMDNJ.EDU [130.219.34.124])
} } } } 8, 32 -- by ns.microscopy.com
} } (8.12.11/8.12.8) with
} } } } ESMTP id j7JGxjmx017595
} } } } 8, 32 -- for
} } {microscopy-at-msa.microscopy.com} ; Fri,
} } } } 19 Aug 2005 11:59:45 -0500
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9, 19 -- From jtwilley-at-sprynet.com Sat Aug 20 11:39:20 2005
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I am not certain what your point is. All i was doing
was pointing out to you that NJ has regs on how to
deal with spent fixer. I was not suggesting that
anyone cheat on proper wast disposal. Your suggestion
that I was is offensive.
FYI I know why silver is prohibited. Don't put words
in my emails that are not there. I already have enough
trouble typing.

As far as I am concerned labs should go digital. Alot
of major hospitals already have.
John Hoffpauit

--- John Twilley {jtwilley-at-sprynet.com} wrote:

} So your point is? "Just do it if you can get away
} with it" and can point to
} something else that's worse?
}
} I pointed out, off list, to the original inquirer
} that most jurisdictions limit
} silver (in spent fixer) because it is a biocide that
} can cause a die-off of the
} bacteria that are required for the normal
} functioning of waste water treatment
} plants.
}
} As an alternative to paying a waste hauler to remove
} fixer, one can sign up a
} precious metal recovery company to remove it.
} Usually this is a break-even
} proposition with the recovery firm providing a
} container and pickup service in
} exchange for keeping the silver and handling the
} disposal of the silver-free
} remains.
}
} John
}
} john hoffpauir wrote:
}
} } Perhaps I can put this in perspective for you.
} Most
} } states release some raw sewage. It generally
} happens
} } by accident. Either a broken pipe or a malfuntion
} of
} } the waste plant, It is not just NJ. I am not an
} } apologist for NJ so how the flames please,
} }
} } As for the NJ reg, I started work in NJ over 15
} years
} } ago and it was a reg back then, not sure if that
} is a
} } generation or not. You tell me. Some states, I was
} } working in Dallas for a lot of years allowed fixer
} to
} } be dumped down the drain when I was working there.
} } Perhaps some one from Texas can tell me if this is
} } still alowed.
} }
} } I should note that mercury is released into the
} water
} } constantly, it is a by product of coal plants
} } producing energy. How many of us recycle batteries
} or
} } just throw them in the trash and forget about
} them.
} } there is no state that requires we recycle them.
} }
} } --- John Twilley {jtwilley-at-sprynet.com} wrote:
} }
} } } According to a recent article in the NYT, New
} Jersey
} } } still has annual releases of raw sewage into the
} } } local waterways annually in the millions of
} gallons.
} } } So perhaps it has not been long that
} } } they have had restrictions. In most parts of
} the
} } } country dissolved metals haven't been permitted
} to
} } } be dumped down the drain in a generation.
} } }
} } } John
} } }
} } } hoffpajo-at-yahoo.com wrote:
} } }
} } } }
} } }
} }
}
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} } } Microscopy Society of America
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} } } }
} } } } i remember those days when i worked in NJ, it
} was
} } } a
} } } } pain in the but to collect all the spent
} fixer.
} } } you
} } } } are right about kodax and the silver recovery
} kit.
} } } } someone else used to do it as well but alas
} that
} } } brain
} } } } cell is long gone.
} } } } i do believe if you truly just want the silver
} in
} } } it's
} } } } raw form you can use steel wool so the silver
} will
} } } } react with the steel wool and bind to it.
} perhaps
} } } some
} } } } on the list server can explain the chemistry
} to us
} } } } all. i was never any good at inorganic
} chemisrty.
} } } } john hoffpauir
} } } }
} } } } --- mcauliff-at-umdnj.edu wrote:
} } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } }
} } }
} }
}
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} } } } } Microscopy Society of America
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} } } } }
} } } }
} } }
} }
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} } } } }
} } } }
} } }
} }
}
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} } } } }
} } } } } Here in New Jersey we are required to
} dispose of
} } } } } used fixer as hazardous
} } } } } waste, due to the silver content. Kodak
} sells
} } } (or
} } } } } used to sell) a silver
} } } } } recovery kit. so you might try them.
} } } } }
} } } } } Geoff
} } } } }
} } } } } Stacey.Andringa-at-uc.edu wrote:
} } } } }
} } } } } }
} } } } }
} } } }
} } }
} }
}
} ----------------------------------------------------------------------------
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} The
} } } } } Microscopy Society of America
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} } }
} }
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} } } } } } Below is the result of your feedback form
} } } } } (NJZFM-ultra-55). It was submitted by
} } } } } (Stacey.Andringa-at-uc.edu) from
} } } } } http://www.microscopy.com/MLFormMail.html on
} } } } } Thursday, August 18, 2005 at 09:57:24
} } } } }
} } } }
} } }
} }
}
} ---------------------------------------------------------------------------
} } } } } }
} } } } } } Email: Stacey.Andringa-at-uc.edu
} } } } } } Name: Stacey Andringa
} } } } } }
} } } } } } Organization: University of Cincinnati
} } } } } }
} } } } } } Title-Subject: [Filtered] MListserver:
} } } } } }
} } } } } } Question: I have been catching up on some
} lab
} } } } } cleaning and have about 12 gallons of spent
}
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==============================Original Headers==============================
7, 20 -- From hoffpajo-at-yahoo.com Sat Aug 20 11:57:20 2005
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Hi

You may find that you are able to punch through the surface deformation by
increasing the kV. The backscatter contribution to the image will increase
and if you are lucky, and the surface damage does not go too
deep, you may see striations more clearly.

Good Luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
X-from: {LDUH-at-SIKORSKY.COM}
To: {protrain-at-emcourses.com}
Sent: Friday, August 19, 2005 11:51 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pollingmel-at-aol.com) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Sunday, August 21, 2005 at 16:30:13
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Email: pollingmel-at-aol.com
Name: Mel Pollinger

Organization: New York Microscopical Society

Title-Subject: [Filtered] MListserver:

Question: Anyone know anything about a product called Geffner Pintubes? They are glass tubes, 7mm O.D. X 10cm L. flame sealed at both ends. Got a bunch of these recently, but have no idea what they are or were for.

Thanks for any clue,
Mel Pollinger

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7, 12 -- From zaluzec-at-microscopy.com Mon Aug 22 07:58:20 2005
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Monday, August 22, 2005 at 04:07:43
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: k.venner

Organization: ucl London uk

Title-Subject: [Filtered] MListserver: TEM tissue processors

Question: We have had a Lynx for nearly 20 years, and during that time I have used it at least once a week, sometimes 2 or 3 times per week. It has needed a replacement mother board once, and we have replaced the vial seal a couple of times. We only go up to 1:1 resin/propylene oxide as we found the neat resin step messy, so we unload at the end of the 1:1 stage into clean processing vials with neat resin. We found it too risky to do unattended runs overnight, as once a vial was misaligned and the samples dried out as the carousel was unable to move to the next vial, but that was operator error rather than a fault with the equipment. We also found that the propylene oxide evaporated too quickly for an overnight run. We do re-use the vials and I clean the baskets and vials with acetone, which works really well, only discarding them when they become too black or loose in the carousel.

I really like the Lynx, and we give space to a newer second one, which is also really handy for the future....

I have seen the Leica version, but have no experience using the machine. They are pretty much similar in design, and the consumables are virtually interchangeable.

It is economic with chemicals and in our hands, reliable. The only limit is the size and dimensions of your samples you want to process.

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Hi

I know many of the UK microscopists read the listserver so here is my plea!

I am looking for a material science laboratory in the UK that uses an FEI
SEM and EDS. I have two material science overseas customers who wish to
take an advanced SEM + EDS training course in the UK and unfortunately all
of my clients with these instruments are biologists!

In exchange for letting us use the instrument we are willing to offer two
places on the course for the laboratory to use. We would need the
microscope each afternoon for 5 days and a small seminar room or office each
morning for the lectures.

If the establishment wishes we are able to carry out the lecture side of the
course for as many people as they may wish to attend, only the practicals
periods would be limited in numbers.

Thanks listserver sorry to take up space.

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

==============================Original Headers==============================
8, 22 -- From protrain-at-emcourses.com Mon Aug 22 13:44:24 2005
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8, 22 -- Subject: FEI SEM Venue Wanted in UK
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Our staff cytopathologist was wondering if there was an EM fixative
available that was similar to "Cytorich Red" fixative for light microscopy.
This formalin based fixative lyses the red blood cells from the fine needle
aspiration biopsies and leaves the tumor cells for the cell blocks and
histology work. He wanted to try this for his EM specimens, but
unfortunately this contains formaldehyde, not gluteraldehyde.

Is there something similar out there for EM?

Thanks, Peggy Harger-Allen

EM Lab

==============================Original Headers==============================
4, 17 -- From Margaret.HargerAllen-at-med.va.gov Mon Aug 22 13:56:06 2005
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4, 17 -- From: "Harger-Allen, Margaret" {Margaret.HargerAllen-at-med.va.gov}
4, 17 -- To: microscopy-at-microscopy.com
4, 17 -- Subject: TEM of aspiration biopsies - remove blood
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pbarber-at-bu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, August 22, 2005 at 11:05:10
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Email: pbarber-at-bu.edu
Name: Paul Barber

Organization: Boston University

Title-Subject: [Filtered] MListserver:

Question: I would like to be able to attach a digital SLR (ie Nikon D100) to a c-mount on a stereo microscope like a Leica MZ9.5 or Nikon SMZ800. I've heard conflicting answers from product reps, some saying that it isn't possible, some saying it is? However, I have no experience at all microscopy, but need to buy a system that has at least some photodocumentation ability. I don't need (and can't afford) a 4-5000 dollar imaging system. It seems like putting a digital SLR on a c-mount would be a reasonable and less expensive way to do photodocumentation.

Thanks
Paul

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ramesh-nair-at-uiowa.edu) from http://www.microscopy.com/MLFormMail.html on Monday, August 22, 2005 at 14:41:19
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Email: ramesh-nair-at-uiowa.edu
Name: Ramesh Nair

Organization: University of Iowa

Title-Subject: [Filtered] EM technologist position oprn

Question: Our EM tech is retiring in November. Therefore a position for EM tech is available. If anybody is interested please contact me:

Ramesh Nair, M.D.
Director, Renal Pathology/EM service
University of Iowa

319 330 3102
ramesh-nair-at-uiowa.edu

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9, 12 -- Subject: viaWWW: EM technologist position open
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Henrik.Kaker-at-guest.arnes.si) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 23, 2005 at 04:17:40
---------------------------------------------------------------------------

Email: Henrik.Kaker-at-guest.arnes.si
Name: Henrik Kaker

Organization: Metal Ravne

Title-Subject: [Filtered] Jeol JSM 35-CF scan generator

Question: Hello All,

We are looking the information about the name and pin configurations of connector(External Beam Interface) on the Jeol JSM 35-CF scan generator. We wish to connect external scan generator.

Thank you,

Henrik Kaker
SEM-EDS Lab
Metal Ravne
Slovenia

---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Tue Aug 23 07:28:40 2005
10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: Henrik.Kaker-at-guest.arnes.si (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: Jeol JSM 35-CF scan generator
10, 12 -- Content-Type: text/plain; charset="us-ascii"
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Sending again.... I figure many folks were in HI.

----------------------------------------------

I am again looking for prepared and interesting
specimens for SEM/STEM or just TEM negs. Human pathogens
are especially welcome. Standard 12mm pin stubs for
SEM are perfect. Bacteria, parasites, cancers, etc.
are good. CPD/fixed specimens coated or un-coated
are fine.

Pls contact me off-line for my payment schedule
and what you may have to sell (I keep it) or rent
(in case you want it back).

gary g.

==============================Original Headers==============================
6, 17 -- From gary-at-gaugler.com Tue Aug 23 10:59:08 2005
6, 17 -- Received: from smtp2.mc.surewest.net (smtp2.mc.surewest.net [66.60.130.51])
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6, 17 -- Received: from unknown (66.60.171.211)
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6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14
6, 17 -- Date: Tue, 23 Aug 2005 08:59:08 -0700
6, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
6, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
6, 17 -- Subject: Trolling for SEM/STEM specimens
6, 17 -- Cc: zaluzec-at-microscopy.com
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (brian.keller-at-sw.ca) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 23, 2005 at 14:27:50
---------------------------------------------------------------------------

Email: brian.keller-at-sw.ca
Name: Brian

Organization: Hospital

Title-Subject: [Filtered] MListserver: microdensitometer

Question: I am interested in purchasing a high resolution microdensitometer for analyzing x-ray films. Can anyone suggest some company's for me. Thanks for any help towards this.

Regards,
Brian

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Tue Aug 23 17:55:56 2005
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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7, 12 -- To: microscopy-at-microscopy.com
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7, 12 -- Subject: viaWWW: microdensitometer
7, 12 -- Content-Type: text/plain; charset="us-ascii"
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I use Macbeth densitometers and like them very much.
While I have an old one, there are newer versions
available. Check out:

http://usa.gretagmacbethstore.com/

I think that these have been a big standard for
a long time.

gary g.

At 03:59 PM 8/23/2005, you wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
10, 19 -- From gary-at-gaugler.com Tue Aug 23 19:32:28 2005
10, 19 -- Received: from smtp2.mc.surewest.net (smtp2.mc.surewest.net [66.60.130.51])
10, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j7O0WSLd024539
10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 23 Aug 2005 19:32:28 -0500
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10, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14
10, 19 -- Date: Tue, 23 Aug 2005 16:17:33 -0700
10, 19 -- To: brian.keller-at-sw.ca
10, 19 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 19 -- Subject: Re: [Microscopy] viaWWW: microdensitometer
10, 19 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 19 -- In-Reply-To: {200508232259.j7NMxSRj019268-at-ns.microscopy.com}
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kunli218-at-yahoo.com.sg) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 24, 2005 at 08:42:53
---------------------------------------------------------------------------

Email: kunli218-at-yahoo.com.sg
Name: Simon Lee

Title-Subject: [Filtered] MListserver: TiAL alloy/intermetallic electrical resistance

Question: Dear Listers,

I'd like to know the electricla resitance of TiAL alloy with different Al content? Any reference to recommend? Is it necessary for TiAL alloy or intermetallic to form at very high temperature (} 800 C) or is it possible for TiAl alloy to form at relatively low temperature (say 400 C)?

Your help is appreciated!1

Simon

Chartered Semicon

---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Wed Aug 24 08:48:10 2005
9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j7ODm9UP008331
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9, 12 -- Mime-Version: 1.0
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9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: kunli218-at-yahoo.com.sg (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: TiAL alloy/intermetallic electrical resistance
9, 12 -- Content-Type: text/plain; charset="us-ascii"
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--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

} Dear John,
}
} Nestor has established a special filter to block all
} my messages not only
} to the ListServer but also emails addressed
} personally to him. He also
} denied me an explanation of what I did wrong.
} Therefore, I would like to
} ask the EM community to help me reinstate my name
} and membership at
} ListServer. If you feel comfortable, I would like
} to ask you to publish my
} letter on ListServer. I don't want to cause you any
} problems, so I suggest
} that if you decide to publish my letter, ask
} Nestor's permission first. I
} would also appreciate your opinion on this letter
} and would be happy to
} make corrections, clarifications, etc. Please feel
} free to tell me if this
} idea doesn't seem suitable and you would prefer not
} to participate. It's
} perfectly fine; I'll find other way to make my
} letter available to EM
} community. I would like to make it clear to my
} fellow microscopists that
} they may be treated in a similar way on ListServer
} if something is not
} done. For this reason, I want to speak directly to
} the people on the
} ListServer, not just Nestor. I also sincerely want
} clarification on what I
} did wrong and why I was boycotted in such harsh way.
} Thanks for your
} understanding and help. Sergey.
}
}
==============================================================
}
} Open letter to EM community:
}
}
} August
} 23, 2005
}
} Dear colleagues,
}
} It will soon be one month since I was disconnected
} from the EM
} community. I am asking for you to support
} reinstatement of my name and
} membership.
}
} As many of you are aware, Nestor cancelled my
} subscription to the
} Microscopy ListServer without a sufficiently clear
} explanation as to what I
} did wrong to the degree that cancellation of my
} membership was necessary. I
} didn't start the thread in question or participate
} in the thread, but
} commented only on the direction of the thread.
}
} After my membership was cancelled, I sent a private
} letter to Nestor asking
} him to explain in detail what was wrong in my recent
} postings, so I could
} adjust my behavior accordingly. He declined to
} answer my questions and
} immediately after installed a filter to block my
} messages. Since I lost
} the ability to communicate with Nestor directly, I
} am asking for your
} support in helping me to reinstate my membership at
} the EM ListServer.
}
} Many of you sent me copies of your emails to the
} ListServer asking Nestor
} to reinstate my membership. Many thanks for that
} support. I sincerely
} believe that a public place, especially a scientific
} forum such as the
} Microscopy ListServer, should not be ruled by any
} single person. Decisions
} should be made based on agreed upon principles that
} are established by the
} EM community. Please help me to reinstate my name
} and membership at
} ListServer.
}
} Thanks for your support. Sergey
}
}
} Here is the correspondence between Nestor and me
} that was on the ListServer
} and which presumably led to my membership
} cancellation. The most recent
} emails are on top. For simplicity's sake, I deleted
} duplicate email repeats
} and most headers. The original messages may be
} obtained from me or through
} ListServer:
}
} Tue Jul 26 11:51:32 2005
} I apologize to the list for posting Marilyn vos
} Savant's statement on
} microscopy. I just wanted to share the article
} because I thought it was
} humorous...I shall refrain from such postings.
} best,
} Beth
}
}
------------------------------------------------------------------------------------------------------------------
} 7/26/2005
} Beth & everyone else
}
} There was no problem with Beth's initial posting
} and no apology was
} needed here Beth. Sorry if I appeared to have come
} down on you.
} The followups IMHO were starting to drift into
} critiques of Marilyn whomever
} and her "crap Journalism" and I was trying to nip
} that direction of
} critique in the bud. However, I see I had the
} opposite effect. Oh well.....
} As Dorrance said, everyone can use a grin
} occassionally .
}
} Nestor
}
}
---------------------------------------------------------------------------------------------------
}
} Nestor
} I don't see any reason why we could not discuss the
} quality of somebody's
} work even if it's a journalist with former (?) high
} IQ. Is it prohibited
} to discuss people's work with high IQ on this
} ListServer? Sergey
}
--------------------------------------------------------------------------------------------
}
} 05:10 PM 7/26/2005
} Sergey
} A honest discussion or even disagreement about a
} subject or work which is
} microscopy
} related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally
} disparage any person or
} organization on the Listserver. This rule applies
} equally to Journalists
} as well as Microscopists. Please review the rules
} which you received
} upon subscription.
}
{http://microscopy.com/MicroscopyListserver/Rules.html} http://microscopy.com/MicroscopyListserver/Rules.html
} Presumably a person of hi IQ has the ability to
} distinguish between these two.
} Nestor
} Your Friendly Neighborhood SysOp
}
}
---------------------------------------------------------------------------------------------------------------------
}
} 7/26/05
} My IQ is very low, Nestor, so I am electron
} microscopist for last 30
} years... Is against rules to say truth? You know,
} truth sometime hurts
} and may be politically incorrect... This forum
} becomes too much politically
} correct and heavily censured (yes, it's true), so I
} am loosing interest
} here... I had enough censorship and "rules" in
} USSR. But, I have to admit
} US is over performing USSR now, so I feel I am at
} home. Sergey
}
-------------------------------------------------------------------------------------------------
}
} Date: Tue, 26 Jul 2005 21:02:29 -0500
} To: sryazant-at-ucla.edu
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} Subject: [Microscopy] Re: Discussion of high IQ vs
} Disparagement of a
}
} Sergy
}
} I don't care who you are or where your from, but I
} insist the rules which
} have been established for over a decade are
} followed. Since you believe
} you are above the rules so be it. You are welcome
} togo elsewhere.
}
} I have canceled your subscription to the Listserver.
}
}
} Nestor
}
}
---------------------------------------------------------------------------------------------------------------------
}
} 7:36 PM 7/26/05
}
=== message truncated ===

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
6, 20 -- From hoffpajo-at-yahoo.com Wed Aug 24 12:43:53 2005
6, 20 -- Received: from web50207.mail.yahoo.com (web50207.mail.yahoo.com [206.190.38.48])
6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j7OHhro7020041
6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 24 Aug 2005 12:43:53 -0500
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6, 20 -- h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
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6, 20 -- Message-ID: {20050824174352.19955.qmail-at-web50207.mail.yahoo.com}
6, 20 -- Received: from [68.32.53.160] by web50207.mail.yahoo.com via HTTP; Wed, 24 Aug 2005 10:43:52 PDT
6, 20 -- Date: Wed, 24 Aug 2005 10:43:52 -0700 (PDT)
6, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 20 -- Subject: Re: [Microscopy] Discussion of high IQ vs Disparagement of a
6, 20 -- To: microscopy-at-microscopy.com
6, 20 -- Cc: zaluzec-at-microscopy.com
6, 20 -- In-Reply-To: {6.1.2.0.2.20050823233808.055a16f0-at-mail.ucla.edu}
6, 20 -- MIME-Version: 1.0
6, 20 -- Content-Type: text/plain; charset=iso-8859-1
6, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================

Contents Retrieved from Microscopy Listserver Archives
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IMHO it's a sad state of affairs that I am afraid to make any bold statement
for fear that I too will be censured, thrown off the Microscopy List and
denied any forum for appeal. Kind of mimics the current climate in our
national government....If this turns out to be my last communication, I want
to thank you all for your open and willing sharing of so much microscopy
knowledge. --Jane LaGoy

--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

} Dear John,
}
} Nestor has established a special filter to block all
} my messages not only
} to the ListServer but also emails addressed
} personally to him. He also
} denied me an explanation of what I did wrong.
} Therefore, I would like to
} ask the EM community to help me reinstate my name
} and membership at
} ListServer. If you feel comfortable, I would like
} to ask you to publish my
} letter on ListServer. I don't want to cause you any
} problems, so I suggest
} that if you decide to publish my letter, ask
} Nestor's permission first. I
} would also appreciate your opinion on this letter
} and would be happy to
} make corrections, clarifications, etc. Please feel
} free to tell me if this
} idea doesn't seem suitable and you would prefer not
} to participate. It's
} perfectly fine; I'll find other way to make my
} letter available to EM
} community. I would like to make it clear to my
} fellow microscopists that
} they may be treated in a similar way on ListServer
} if something is not
} done. For this reason, I want to speak directly to
} the people on the
} ListServer, not just Nestor. I also sincerely want
} clarification on what I
} did wrong and why I was boycotted in such harsh way.
} Thanks for your
} understanding and help. Sergey.
}
==============================================================
}
} Open letter to EM community:
}
}
} August 23, 2005
}
} Dear colleagues,
}
} It will soon be one month since I was disconnected
} from the EM
} community. I am asking for you to support
} reinstatement of my name and membership.
}
} As many of you are aware, Nestor cancelled my
} subscription to the
} Microscopy ListServer without a sufficiently clear
} explanation as to what I
} did wrong to the degree that cancellation of my
} membership was necessary. I
} didn't start the thread in question or participate
} in the thread, but
} commented only on the direction of the thread.
}
} After my membership was cancelled, I sent a private
} letter to Nestor asking
} him to explain in detail what was wrong in my recent
} postings, so I could
} adjust my behavior accordingly. He declined to
} answer my questions and
} immediately after installed a filter to block my
} messages. Since I lost
} the ability to communicate with Nestor directly, I
} am asking for your
} support in helping me to reinstate my membership at
} the EM ListServer.
}
} Many of you sent me copies of your emails to the
} ListServer asking Nestor
} to reinstate my membership. Many thanks for that
} support. I sincerely
} believe that a public place, especially a scientific
} forum such as the
} Microscopy ListServer, should not be ruled by any
} single person. Decisions
} should be made based on agreed upon principles that
} are established by the
} EM community. Please help me to reinstate my name
} and membership at ListServer.
}
} Thanks for your support. Sergey
}
}
} Here is the correspondence between Nestor and me
} that was on the ListServer
} and which presumably led to my membership
} cancellation. The most recent
} emails are on top. For simplicity's sake, I deleted
} duplicate email repeats
} and most headers. The original messages may be
} obtained from me or through ListServer:
}
} Tue Jul 26 11:51:32 2005
} I apologize to the list for posting Marilyn vos
} Savant's statement on
} microscopy. I just wanted to share the article
} because I thought it was
} humorous...I shall refrain from such postings.
} best, Beth
}
----------------------------------------------------------------------------
---------------------
} 7/26/2005
} Beth & everyone else
}
} There was no problem with Beth's initial posting
} and no apology was
} needed here Beth. Sorry if I appeared to have come
} down on you.
} The followups IMHO were starting to drift into
} critiques of Marilyn whomever
} and her "crap Journalism" and I was trying to nip
} that direction of
} critique in the bud. However, I see I had the
} opposite effect. Oh well.....
} As Dorrance said, everyone can use a grin
} occassionally.
}
} Nestor
}
----------------------------------------------------------------------------
---------------------}
} Nestor
} I don't see any reason why we could not discuss the
} quality of somebody's
} work even if it's a journalist with former (?) high
} IQ. Is it prohibited
} to discuss people's work with high IQ on this
} ListServer? Sergey
}
----------------------------------------------------------------------------
----------------
}
} 05:10 PM 7/26/2005
} Sergey
} A honest discussion or even disagreement about a
} subject or work which is
} microscopy
} related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally
} disparage any person or
} organization on the Listserver. This rule applies
} equally to Journalists
} as well as Microscopists. Please review the rules
} which you received upon subscription.
}
{http://microscopy.com/MicroscopyListserver/Rules.html} http://microscopy.com
/MicroscopyListserver/Rules.html
} Presumably a person of hi IQ has the ability to
} distinguish between these two.
} Nestor
} Your Friendly Neighborhood SysOp
}
----------------------------------------------------------------------------
---------------------
}
} 7/26/05
} My IQ is very low, Nestor, so I am electron
} microscopist for last 30
} years... Is against rules to say truth? You know,
} truth sometime hurts
} and may be politically incorrect... This forum
} becomes too much politically
} correct and heavily censured (yes, it's true), so I
} am loosing interest
} here... I had enough censorship and "rules" in
} USSR. But, I have to admit
} US is over performing USSR now, so I feel I am at
} home. Sergey
}
----------------------------------------------------------------------------
---------------------
}
} Date: Tue, 26 Jul 2005 21:02:29 -0500
} To: sryazant-at-ucla.edu
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} Subject: [Microscopy] Re: Discussion of high IQ vs
} Disparagement of a
}
} Sergy
}
} I don't care who you are or where your from, but I
} insist the rules which
} have been established for over a decade are
} followed. Since you believe
} you are above the rules so be it. You are welcome
} to go elsewhere.
}
} I have canceled your subscription to the Listserver.
}
} Nestor
}

==============================Original Headers==============================
3, 16 -- From Jane.LaGoy-at-bodycote.com Wed Aug 24 13:54:21 2005
3, 16 -- Received: from Exchange.BODYCOTE-IMT.COM (mail.bodycote-imt.com [12.30.23.178])
3, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j7OIsLVq028656
3, 16 -- for {Microscopy-at-MSA.Microscopy.com} ; Wed, 24 Aug 2005 13:54:21 -0500
3, 16 -- Received: by mail.bodycote-imt.com with Internet Mail Service (5.5.2657.72)
3, 16 -- id {RN8PX328} ; Wed, 24 Aug 2005 14:56:22 -0400
3, 16 -- Message-ID: {CBB9714FDC67D411B39400D0B73C4B73023370EC-at-mail.bodycote-imt.com}
3, 16 -- From: Jane LaGoy {Jane.LaGoy-at-bodycote.com}
3, 16 -- To: "Microscopy Listserver (E-mail)" {Microscopy-at-MSA.Microscopy.com}
3, 16 -- Cc: "'sryazant-at-ucla.edu'" {sryazant-at-ucla.edu}
3, 16 -- Subject: freedom of speech?
3, 16 -- Date: Wed, 24 Aug 2005 14:56:20 -0400
3, 16 -- MIME-Version: 1.0
3, 16 -- X-Mailer: Internet Mail Service (5.5.2657.72)
3, 16 -- Content-Type: text/plain;
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I think Sergey got what he seemed to be asking for.

It was just absurd, as well as personally insulting to Nestor, to say that this
forum is "politically correct and heavily censured (sic)".

And he did say he was "loosing (sic) interest here".

I don't think that you, John, do either yourself or Sergey any favors by sending
this to the list. I don't think this should snowball.

I and most listers, belong to it because we want to participate in discussions
about microscopy, not listen to these little intrigues, vendettas, and exhibitions
of paranoia.

As I have said before, it's not an internet chat room.

rtch

} ----------------------------------------------- } } 7/26/05 } My IQ is
} very low, Nestor, so I am electron } microscopist for last 30 }
} years... Is against rules to say truth? You know, } truth sometime
} hurts } and may be politically incorrect... This forum } becomes too
} much politically } correct and heavily censured (yes, it's true), so I
} } am loosing interest } here... I had enough censorship and "rules"
} in } USSR. But, I have to admit } US is over performing USSR now, so I
} feel I am at } home. Sergey }
} ----------------------------------------------------------------------

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand

==============================Original Headers==============================
13, 27 -- From r.sims-at-auckland.ac.nz Wed Aug 24 15:49:46 2005
13, 27 -- Received: from smtpa.itss.auckland.ac.nz (groucho.itss.auckland.ac.nz [130.216.190.11])
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13, 27 -- Organization: Dept of Geology, Univ of Auckland
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13, 27 -- Date: Thu, 25 Aug 2005 08:51:13 +1200
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Nestor,

Please explain to the list why you removed Sergey. Thank you.

David A. Foran, chemist
Food and Drug Administration
Kansas City Elemental Analysis Lab
DFORAN-at-ORA.FDA.GOV
913-752-2170
913-752-2151 (fax)

-----Original Message-----
X-from: Jane.LaGoy-at-bodycote.com [mailto:Jane.LaGoy-at-bodycote.com]
Sent: Wednesday, August 24, 2005 1:56 PM
To: Foran, David A

IMHO it's a sad state of affairs that I am afraid to make any bold statement
for fear that I too will be censured, thrown off the Microscopy List and
denied any forum for appeal. Kind of mimics the current climate in our
national government....If this turns out to be my last communication, I want
to thank you all for your open and willing sharing of so much microscopy
knowledge. --Jane LaGoy

--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

} Dear John,
}
} Nestor has established a special filter to block all
} my messages not only
} to the ListServer but also emails addressed
} personally to him. He also
} denied me an explanation of what I did wrong.
} Therefore, I would like to
} ask the EM community to help me reinstate my name
} and membership at
} ListServer. If you feel comfortable, I would like
} to ask you to publish my
} letter on ListServer. I don't want to cause you any
} problems, so I suggest
} that if you decide to publish my letter, ask
} Nestor's permission first. I
} would also appreciate your opinion on this letter
} and would be happy to
} make corrections, clarifications, etc. Please feel
} free to tell me if this
} idea doesn't seem suitable and you would prefer not
} to participate. It's
} perfectly fine; I'll find other way to make my
} letter available to EM
} community. I would like to make it clear to my
} fellow microscopists that
} they may be treated in a similar way on ListServer
} if something is not
} done. For this reason, I want to speak directly to
} the people on the
} ListServer, not just Nestor. I also sincerely want
} clarification on what I
} did wrong and why I was boycotted in such harsh way.
} Thanks for your
} understanding and help. Sergey.
}
==============================================================
}
} Open letter to EM community:
}
}
} August 23, 2005
}
} Dear colleagues,
}
} It will soon be one month since I was disconnected
} from the EM
} community. I am asking for you to support
} reinstatement of my name and membership.
}
} As many of you are aware, Nestor cancelled my
} subscription to the
} Microscopy ListServer without a sufficiently clear
} explanation as to what I
} did wrong to the degree that cancellation of my
} membership was necessary. I
} didn't start the thread in question or participate
} in the thread, but
} commented only on the direction of the thread.
}
} After my membership was cancelled, I sent a private
} letter to Nestor asking
} him to explain in detail what was wrong in my recent
} postings, so I could
} adjust my behavior accordingly. He declined to
} answer my questions and
} immediately after installed a filter to block my
} messages. Since I lost
} the ability to communicate with Nestor directly, I
} am asking for your
} support in helping me to reinstate my membership at
} the EM ListServer.
}
} Many of you sent me copies of your emails to the
} ListServer asking Nestor
} to reinstate my membership. Many thanks for that
} support. I sincerely
} believe that a public place, especially a scientific
} forum such as the
} Microscopy ListServer, should not be ruled by any
} single person. Decisions
} should be made based on agreed upon principles that
} are established by the
} EM community. Please help me to reinstate my name
} and membership at ListServer.
}
} Thanks for your support. Sergey
}
}
} Here is the correspondence between Nestor and me
} that was on the ListServer
} and which presumably led to my membership
} cancellation. The most recent
} emails are on top. For simplicity's sake, I deleted
} duplicate email repeats
} and most headers. The original messages may be
} obtained from me or through ListServer:
}
} Tue Jul 26 11:51:32 2005
} I apologize to the list for posting Marilyn vos
} Savant's statement on
} microscopy. I just wanted to share the article
} because I thought it was
} humorous...I shall refrain from such postings.
} best, Beth
}
----------------------------------------------------------------------------
---------------------
} 7/26/2005
} Beth & everyone else
}
} There was no problem with Beth's initial posting
} and no apology was
} needed here Beth. Sorry if I appeared to have come
} down on you.
} The followups IMHO were starting to drift into
} critiques of Marilyn whomever
} and her "crap Journalism" and I was trying to nip
} that direction of
} critique in the bud. However, I see I had the
} opposite effect. Oh well.....
} As Dorrance said, everyone can use a grin
} occassionally.
}
} Nestor
}
----------------------------------------------------------------------------
---------------------}
} Nestor
} I don't see any reason why we could not discuss the
} quality of somebody's
} work even if it's a journalist with former (?) high
} IQ. Is it prohibited
} to discuss people's work with high IQ on this
} ListServer? Sergey
}
----------------------------------------------------------------------------
----------------
}
} 05:10 PM 7/26/2005
} Sergey
} A honest discussion or even disagreement about a
} subject or work which is
} microscopy
} related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally
} disparage any person or
} organization on the Listserver. This rule applies
} equally to Journalists
} as well as Microscopists. Please review the rules
} which you received upon subscription.
}
{http://microscopy.com/MicroscopyListserver/Rules.html} http://microscopy.com
/MicroscopyListserver/Rules.html
} Presumably a person of hi IQ has the ability to
} distinguish between these two.
} Nestor
} Your Friendly Neighborhood SysOp
}
----------------------------------------------------------------------------
---------------------
}
} 7/26/05
} My IQ is very low, Nestor, so I am electron
} microscopist for last 30
} years... Is against rules to say truth? You know,
} truth sometime hurts
} and may be politically incorrect... This forum
} becomes too much politically
} correct and heavily censured (yes, it's true), so I
} am loosing interest
} here... I had enough censorship and "rules" in
} USSR. But, I have to admit
} US is over performing USSR now, so I feel I am at
} home. Sergey
}
----------------------------------------------------------------------------
---------------------
}
} Date: Tue, 26 Jul 2005 21:02:29 -0500
} To: sryazant-at-ucla.edu
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} Subject: [Microscopy] Re: Discussion of high IQ vs Disparagement of a
}
} Sergy
}
} I don't care who you are or where your from, but I
} insist the rules which
} have been established for over a decade are
} followed. Since you believe
} you are above the rules so be it. You are welcome
} to go elsewhere.
}
} I have canceled your subscription to the Listserver.
}
} Nestor
}

==============================Original Headers==============================
3, 16 -- From Jane.LaGoy-at-bodycote.com Wed Aug 24 13:54:21 2005 3, 16 --
Received: from Exchange.BODYCOTE-IMT.COM (mail.bodycote-imt.com
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3, 16 -- To: "Microscopy Listserver (E-mail)"
{Microscopy-at-MSA.Microscopy.com} 3, 16 -- Cc: "'sryazant-at-ucla.edu'"
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==============================Original Headers==============================
13, 16 -- From DFORAN-at-ORA.FDA.GOV Wed Aug 24 15:51:37 2005
13, 16 -- Received: from wallsand-pub.fda.gov (wallsand-pub.fda.gov [150.148.0.31])
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13, 16 -- From: "Foran, David A" {DFORAN-at-ORA.FDA.GOV}
13, 16 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
13, 16 -- Subject: FW: [Microscopy] freedom of speech?
13, 16 -- Date: Wed, 24 Aug 2005 16:50:38 -0400
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Colleagues

As many of you know, an individual's subscription was canceled to
the Listserver
in July , as per the rules of the Microscopy Listserver to which
everyone must
comply in order to maintain their subscription. An unverifiable message was
recently forwarded by a third party to the listserver concerning this matter.

For the record, the subscriber was informed of the cancellation and
instructed that he could apply
for a new subscription after an appointed period of time. There
have been a total of six
individuals to whom this restriction has been applied in the last 12
YEARS of operation
of the Microscopy Listserver. Of the six, all but two have been
fully reinstated and
they have, since that time, compiled with the rules of the
Microscopy Listserver.
The individual in question, should he so choose, will be given the
same opportunity.

I should also note for clarification, that contrary to the
unverifiable statements purported to be
from this individual, there are no special filters placed upon him personally,
only those imposed upon all non-subscribers and SPAM/UCE sources
by automated server
software.

I will remind you that the Listserver is a subscripton based forum
having well defined
and professional purposes, which has rules concerning its use as well as
membership. It is moderated and is monitored for complaince with its rules
with reasonable decorum and professionalism expected at all times
by subscribers.
There are numerous unmoderated public chat rooms which are
available to anyone that is interested in participating in
unbridled open ended public venues.

If you wish to review the Microscopy Listserver Rules, you may find
them on-line at
http://www.microscopy.com/MicroscopyListserver.

Anyone that feels that the Listerver rules are too restrictive for their liking
is welcome to unsubscribe at any time. The electronic forms for this
can also be found
at the above WWW site.

There is no purpose in further discussion on this matter, as
Administrator/SysOp of the
Microscopy Listserver, this decision was mine and mine alone, and
it is final. You
are welcome to address comments to me personally off-line should you
so desire.

I consider this thread closed and suggest that this forum return to
questions/discussions
concerning its purpose i.e. Microscopy & Microanalysis.

Nestor
The Microscopy SysOp

==============================Original Headers==============================
11, 15 -- From zaluzec-at-microscopy.com Wed Aug 24 16:13:35 2005
11, 15 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
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11, 15 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105])
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11, 15 -- Date: Wed, 24 Aug 2005 16:13:33 -0500
11, 15 -- To: microscopy-at-microscopy.com
11, 15 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
11, 15 -- Subject: Administrivia: Listserver Rules
11, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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And right you were.

--- "r. williams" {s2kdude-at-pacbell.net} wrote:

} i predict Nestor will say nothing but a re-iteration
} of his rules.
}
} --- hoffpajo-at-yahoo.com wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------------
} }
} }
} }
} } --- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:
} }
} } } Dear John,
} } }
} } } Nestor has established a special filter to block
} } all
} } } my messages not only
} } } to the ListServer but also emails addressed
} } } personally to him. He also
} } } denied me an explanation of what I did wrong.
} } } Therefore, I would like to
} } } ask the EM community to help me reinstate my
} name
} } } and membership at
} } } ListServer. If you feel comfortable, I would
} like
} } } to ask you to publish my
} } } letter on ListServer. I don't want to cause you
} } any
} } } problems, so I suggest
} } } that if you decide to publish my letter, ask
} } } Nestor's permission first. I
} } } would also appreciate your opinion on this
} letter
} } } and would be happy to
} } } make corrections, clarifications, etc. Please
} } feel
} } } free to tell me if this
} } } idea doesn't seem suitable and you would prefer
} } not
} } } to participate. It's
} } } perfectly fine; I'll find other way to make my
} } } letter available to EM
} } } community. I would like to make it clear to my
} } } fellow microscopists that
} } } they may be treated in a similar way on
} ListServer
} } } if something is not
} } } done. For this reason, I want to speak directly
} } to
} } } the people on the
} } } ListServer, not just Nestor. I also sincerely
} } want
} } } clarification on what I
} } } did wrong and why I was boycotted in such harsh
} } way.
} } } Thanks for your
} } } understanding and help. Sergey.
} } }
} } }
} }
}
==============================================================
} } }
} } } Open letter to EM community:
} } }
} } }
}
} }
} } }
} August
} } } 23, 2005
} } }
} } } Dear colleagues,
} } }
} } } It will soon be one month since I was
} disconnected
} } } from the EM
} } } community. I am asking for you to support
} } } reinstatement of my name and
} } } membership.
} } }
} } } As many of you are aware, Nestor cancelled my
} } } subscription to the
} } } Microscopy ListServer without a sufficiently
} clear
} } } explanation as to what I
} } } did wrong to the degree that cancellation of my
} } } membership was necessary. I
} } } didn't start the thread in question or
} participate
} } } in the thread, but
} } } commented only on the direction of the thread.
} } }
} } } After my membership was cancelled, I sent a
} } private
} } } letter to Nestor asking
} } } him to explain in detail what was wrong in my
} } recent
} } } postings, so I could
} } } adjust my behavior accordingly. He declined to
} } } answer my questions and
} } } immediately after installed a filter to block my
} } } messages. Since I lost
} } } the ability to communicate with Nestor directly,
} I
} } } am asking for your
} } } support in helping me to reinstate my membership
} } at
} } } the EM ListServer.
} } }
} } } Many of you sent me copies of your emails to the
} } } ListServer asking Nestor
} } } to reinstate my membership. Many thanks for
} that
} } } support. I sincerely
} } } believe that a public place, especially a
} } scientific
} } } forum such as the
} } } Microscopy ListServer, should not be ruled by
} any
} } } single person. Decisions
} } } should be made based on agreed upon principles
} } that
} } } are established by the
} } } EM community. Please help me to reinstate my
} name
} } } and membership at
} } } ListServer.
} } }
} } } Thanks for your support. Sergey
} } }
} } }
} } } Here is the correspondence between Nestor and me
} } } that was on the ListServer
} } } and which presumably led to my membership
} } } cancellation. The most recent
} } } emails are on top. For simplicity's sake, I
} } deleted
} } } duplicate email repeats
} } } and most headers. The original messages may be
} } } obtained from me or through
} } } ListServer:
} } }
} } } Tue Jul 26 11:51:32 2005
} } } I apologize to the list for posting Marilyn vos
} } } Savant's statement on
} } } microscopy. I just wanted to share the article
} } } because I thought it was
} } } humorous...I shall refrain from such postings.
} } } best,
} } } Beth
} } }
} } }
} }
}
------------------------------------------------------------------------------------------------------------------
} } } 7/26/2005
} } } Beth & everyone else
} } }
} } } There was no problem with Beth's initial
} posting
} } } and no apology was
} } } needed here Beth. Sorry if I appeared to have
} } come
} } } down on you.
} } } The followups IMHO were starting to drift into
} } } critiques of Marilyn whomever
} } } and her "crap Journalism" and I was trying to
} nip
} } } that direction of
} } } critique in the bud. However, I see I had the
} } } opposite effect. Oh well.....
} } } As Dorrance said, everyone can use a grin
} } } occassionally .
} } }
} } } Nestor
} } }
} } }
} }
}
---------------------------------------------------------------------------------------------------
} } }
} } } Nestor
} } } I don't see any reason why we could not discuss
} } the
} } } quality of somebody's
} } } work even if it's a journalist with former (?)
} } high
} } } IQ. Is it prohibited
} } } to discuss people's work with high IQ on this
} } } ListServer? Sergey
}
=== message truncated ===

__________________________________________________
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==============================Original Headers==============================
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5, 20 -- Date: Wed, 24 Aug 2005 18:14:43 -0700 (PDT)
5, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 20 -- Subject: Re: [Microscopy] Re: Discussion of high IQ vs Disparagement of a
5, 20 -- To: "r. williams" {s2kdude-at-pacbell.net}
5, 20 -- Cc: microscopy-at-microscopy.com
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This will be the last time I respond to your your
email.
I have been to Europe more times than you have been to
the US. I am a world traveler. In fact I will be in
Nice France next week attending a medical conference.
Care to hash it out there. I can give you my exact
hotel and dates I will be there.
John Hoffpauir

--- Matthias M�rgelin {Matthias.Morgelin-at-med.lu.se}
wrote:

} I will neither block you email address or simply
} delete your mails, as you propose, this is not
} necessary, I will just ignore your little rooster
} thing as probably many other grown-up people in this
} forum will do. This is my last response to your
} personal war against the listserver.
} Best regards from Sweden (if you know at all where
} this land is located)
}
} ----- Original Message -----
} From: john hoffpauir {hoffpajo-at-yahoo.com}
} Date: Wednesday, August 24, 2005 10:30 pm
} Subject: Re: [Microscopy] Re: Discussion of high IQ
} vs Disparagement of a
}
} } i am not on an ego trip, but it sure sounds like
} you
} } have some issues to work out. if you don't like
} what i
} } have to say, block my email address or simply
} delete
} } them. try to be a little more open minded.
} } john hoffpauir
} }
} }
} }
} } --- Matthias M�rgelin
} {Matthias.Morgelin-at-med.lu.se}
} } wrote:
} }
} } } ..."so I am loosing interest here"...etc
} } } blablabla.!!!
} } }
} } } This forum has not been established for persons
} like
} } } you to express your very own small personal
} ego-trip
} } } things all the time. Sometimes I really admire
} } } Nestor for his neverending positive attitude
} towards
} } } guys like you! Personally I would have blocked
} you
} } } much earler!
} } }
} } }
} } } ----- Original Message -----
} } } From: hoffpajo-at-yahoo.com
} } } Date: Wednesday, August 24, 2005 7:46 pm
} } } Subject: [Microscopy] Re: Discussion of high IQ
} vs
} } } Disparagement of a
} } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
}
-------------------------------------------------------------------
} } } } ---------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of
} } } } AmericaTo Subscribe/Unsubscribe --
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserverOn-Line
} } } Help
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } } }
} } }
} }
}
----------------------------------------------------------------
} } } }
} } } }
} } } }
} } } } --- Sergey Ryazantsev {sryazant-at-ucla.edu}
} wrote:
} } } }
} } } } } Dear John,
} } } } }
} } } } } Nestor has established a special filter to
} block
} } } all
} } } } } my messages not only
} } } } } to the ListServer but also emails addressed
} } } } } personally to him. He also
} } } } } denied me an explanation of what I did
} wrong.
} } } } } Therefore, I would like to
} } } } } ask the EM community to help me reinstate my
} } } name
} } } } } and membership at
} } } } } ListServer. If you feel comfortable, I
} would
} } } like
} } } } } to ask you to publish my
} } } } } letter on ListServer. I don't want to cause
} you
} } } any
} } } } } problems, so I suggest
} } } } } that if you decide to publish my letter, ask
} } } } } Nestor's permission first. I
} } } } } would also appreciate your opinion on this
} } } letter
} } } } } and would be happy to
} } } } } make corrections, clarifications, etc.
} Please
} } } feel
} } } } } free to tell me if this
} } } } } idea doesn't seem suitable and you would
} prefer
} } } not
} } } } } to participate. It's
} } } } } perfectly fine; I'll find other way to make
} my
} } } } } letter available to EM
} } } } } community. I would like to make it clear to
} my
} } } } } fellow microscopists that
} } } } } they may be treated in a similar way on
} } } ListServer
} } } } } if something is not
} } } } } done. For this reason, I want to speak
} directly
} } } to
} } } } } the people on the
} } } } } ListServer, not just Nestor. I also
} sincerely
} } } want
} } } } } clarification on what I
} } } } } did wrong and why I was boycotted in such
} harsh
} } } way.
} } } } } Thanks for your
} } } } } understanding and help. Sergey.
} } } } }
} } } } }
} } } }
} } }
} }
}
==============================================================
} } } } }
} } } } } Open letter to EM community:
} } } } }
} } } } }
}
} } }
} } } } }
} } } August
} } } } } 23, 2005
} } } } }
} } } } } Dear colleagues,
} } } } }
} } } } } It will soon be one month since I was
} } } disconnected
} } } } } from the EM
} } } } } community. I am asking for you to support
} } } } } reinstatement of my name and
} } } } } membership.
} } } } }
} } } } } As many of you are aware, Nestor cancelled
} my
} } } } } subscription to the
} } } } } Microscopy ListServer without a sufficiently
} } } clear
} } } } } explanation as to what I
} } } } } did wrong to the degree that cancellation of
} my
} } } } } membership was necessary. I
} } } } } didn't start the thread in question or
} } } participate
} } } } } in the thread, but
} } } } } commented only on the direction of the
} thread.
} } } } }
} } } } } After my membership was cancelled, I sent a
} } } private
} } } } } letter to Nestor asking
} } } } } him to explain in detail what was wrong in
} my
} } } recent
} } } } } postings, so I could
} } } } } adjust my behavior accordingly. He declined
} to
} } } } } answer my questions and
} } } } } immediately after installed a filter to
} block my
} } } } } messages. Since I lost
} } } } } the ability to communicate with Nestor
} directly,
} } } I
} } } } } am asking for your
} } } } } support in helping me to reinstate my
} membership
} } } at
} } } } } the EM ListServer.
} } } } }
}
=== message truncated ===

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==============================Original Headers==============================
5, 20 -- From hoffpajo-at-yahoo.com Wed Aug 24 20:28:47 2005
5, 20 -- Received: from web50202.mail.yahoo.com (web50202.mail.yahoo.com [206.190.38.43])
5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j7P1Skg2008543
5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 24 Aug 2005 20:28:46 -0500
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5, 20 -- Date: Wed, 24 Aug 2005 18:28:46 -0700 (PDT)
5, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 20 -- Subject: Re: [Microscopy] Re: Discussion of high IQ vs Disparagement of a
5, 20 -- To: Matthias "M�rgelin" {Matthias.Morgelin-at-med.lu.se}
5, 20 -- Cc: microscopy-at-microscopy.com
5, 20 -- In-Reply-To: {1afb3801af6965.1af69651afb380-at-net.lu.se}
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-Type: text/plain; charset=iso-8859-1
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Contents Retrieved from Microscopy Listserver Archives
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hmmmm is this disparagement? Personal I don't like
fruitcake.

--- Matthias M�rgelin {Matthias.Morgelin-at-med.lu.se}
wrote:

} interesting which fruitcakes you can meet on the
} net. some of them even seem to be psycologists
}
} ----- Original Message -----
} From: john hoffpauir {hoffpajo-at-yahoo.com}
} Date: Wednesday, August 24, 2005 10:30 pm
} Subject: Re: [Microscopy] Re: Discussion of high IQ
} vs Disparagement of a
}
} } i am not on an ego trip, but it sure sounds like
} you
} } have some issues to work out. if you don't like
} what i
} } have to say, block my email address or simply
} delete
} } them. try to be a little more open minded.
} } john hoffpauir
} }
} }
} }
} } --- Matthias M�rgelin
} {Matthias.Morgelin-at-med.lu.se}
} } wrote:
} }
} } } ..."so I am loosing interest here"...etc
} } } blablabla.!!!
} } }
} } } This forum has not been established for persons
} like
} } } you to express your very own small personal
} ego-trip
} } } things all the time. Sometimes I really admire
} } } Nestor for his neverending positive attitude
} towards
} } } guys like you! Personally I would have blocked
} you
} } } much earler!
} } }
} } }
} } } ----- Original Message -----
} } } From: hoffpajo-at-yahoo.com
} } } Date: Wednesday, August 24, 2005 7:46 pm
} } } Subject: [Microscopy] Re: Discussion of high IQ
} vs
} } } Disparagement of a
} } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
}
-------------------------------------------------------------------
} } } } ---------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of
} } } } AmericaTo Subscribe/Unsubscribe --
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserverOn-Line
} } } Help
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } } }
} } }
} }
}
----------------------------------------------------------------
} } } }
} } } }
} } } }
} } } } --- Sergey Ryazantsev {sryazant-at-ucla.edu}
} wrote:
} } } }
} } } } } Dear John,
} } } } }
} } } } } Nestor has established a special filter to
} block
} } } all
} } } } } my messages not only
} } } } } to the ListServer but also emails addressed
} } } } } personally to him. He also
} } } } } denied me an explanation of what I did
} wrong.
} } } } } Therefore, I would like to
} } } } } ask the EM community to help me reinstate my
} } } name
} } } } } and membership at
} } } } } ListServer. If you feel comfortable, I
} would
} } } like
} } } } } to ask you to publish my
} } } } } letter on ListServer. I don't want to cause
} you
} } } any
} } } } } problems, so I suggest
} } } } } that if you decide to publish my letter, ask
} } } } } Nestor's permission first. I
} } } } } would also appreciate your opinion on this
} } } letter
} } } } } and would be happy to
} } } } } make corrections, clarifications, etc.
} Please
} } } feel
} } } } } free to tell me if this
} } } } } idea doesn't seem suitable and you would
} prefer
} } } not
} } } } } to participate. It's
} } } } } perfectly fine; I'll find other way to make
} my
} } } } } letter available to EM
} } } } } community. I would like to make it clear to
} my
} } } } } fellow microscopists that
} } } } } they may be treated in a similar way on
} } } ListServer
} } } } } if something is not
} } } } } done. For this reason, I want to speak
} directly
} } } to
} } } } } the people on the
} } } } } ListServer, not just Nestor. I also
} sincerely
} } } want
} } } } } clarification on what I
} } } } } did wrong and why I was boycotted in such
} harsh
} } } way.
} } } } } Thanks for your
} } } } } understanding and help. Sergey.
} } } } }
} } } } }
} } } }
} } }
} }
}
==============================================================
} } } } }
} } } } } Open letter to EM community:
} } } } }
} } } } }
}
} } }
} } } } }
} } } August
} } } } } 23, 2005
} } } } }
} } } } } Dear colleagues,
} } } } }
} } } } } It will soon be one month since I was
} } } disconnected
} } } } } from the EM
} } } } } community. I am asking for you to support
} } } } } reinstatement of my name and
} } } } } membership.
} } } } }
} } } } } As many of you are aware, Nestor cancelled
} my
} } } } } subscription to the
} } } } } Microscopy ListServer without a sufficiently
} } } clear
} } } } } explanation as to what I
} } } } } did wrong to the degree that cancellation of
} my
} } } } } membership was necessary. I
} } } } } didn't start the thread in question or
} } } participate
} } } } } in the thread, but
} } } } } commented only on the direction of the
} thread.
} } } } }
} } } } } After my membership was cancelled, I sent a
} } } private
} } } } } letter to Nestor asking
} } } } } him to explain in detail what was wrong in
} my
} } } recent
} } } } } postings, so I could
} } } } } adjust my behavior accordingly. He declined
} to
} } } } } answer my questions and
} } } } } immediately after installed a filter to
} block my
} } } } } messages. Since I lost
} } } } } the ability to communicate with Nestor
} directly,
} } } I
} } } } } am asking for your
} } } } } support in helping me to reinstate my
} membership
} } } at
} } } } } the EM ListServer.
} } } } }
} } } } } Many of you sent me copies of your emails to
} the
} } } } } ListServer asking Nestor
} } } } } to reinstate my membership. Many thanks for
} } } that
} } } } } support. I sincerely
}
=== message truncated ===

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Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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==============================Original Headers==============================
6, 20 -- From hoffpajo-at-yahoo.com Wed Aug 24 20:44:16 2005
6, 20 -- Received: from web50208.mail.yahoo.com (web50208.mail.yahoo.com [206.190.38.49])
6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j7P1iGfp016565
6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 24 Aug 2005 20:44:16 -0500
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6, 20 -- Received: from [68.32.53.160] by web50208.mail.yahoo.com via HTTP; Wed, 24 Aug 2005 18:44:16 PDT
6, 20 -- Date: Wed, 24 Aug 2005 18:44:16 -0700 (PDT)
6, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 20 -- Subject: Re: [Microscopy] Re: Discussion of high IQ vs Disparagement of a
6, 20 -- To: Matthias "M�rgelin" {Matthias.Morgelin-at-med.lu.se}
6, 20 -- Cc: microscopy-at-microscopy.com
6, 20 -- In-Reply-To: {1af85be1af6797.1af67971af85be-at-net.lu.se}
6, 20 -- MIME-Version: 1.0
6, 20 -- Content-Type: text/plain; charset=iso-8859-1
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It maybe 1500 miles. I have never been to Stockholm. I
it however over 3000 miles to Nice from Philadelphia,
but I am still going.
John

--- Mike O'Keefe {MAOKeefe-at-lbl.gov} wrote:

} Isn't it, like, 1500 miles from Nice to Stockholm?
}
} ----- Original Message -----
} From: hoffpajo-at-yahoo.com
} Date: Wednesday, August 24, 2005 6:30 pm
} Subject: [Microscopy] Discussion of high IQ vs
} Disparagement of a
}
} }
} }
} }
} }
}
--------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of
} } AmericaTo Subscribe/Unsubscribe --
} }
}
http://www.microscopy.com/MicroscopyListserverOn-Line
} Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html-------------
} }
}
---------------------------------------------------------------
} }
} } This will be the last time I respond to your your
} } email.
} } I have been to Europe more times than you have
} been to
} } the US. I am a world traveler. In fact I will be
} in
} } Nice France next week attending a medical
} conference.
} } Care to hash it out there. I can give you my exact
} } hotel and dates I will be there.
} } John Hoffpauir
} }
} } --- Matthias M�rgelin
} {Matthias.Morgelin-at-med.lu.se}
} } wrote:
} }
} } } I will neither block you email address or simply
} } } delete your mails, as you propose, this is not
} } } necessary, I will just ignore your little
} rooster
} } } thing as probably many other grown-up people in
} this
} } } forum will do. This is my last response to your
} } } personal war against the listserver.
} } } Best regards from Sweden (if you know at all
} where
} } } this land is located)
} } }
} } } ----- Original Message -----
} } } From: john hoffpauir {hoffpajo-at-yahoo.com}
} } } Date: Wednesday, August 24, 2005 10:30 pm
} } } Subject: Re: [Microscopy] Re: Discussion of
} high IQ
} } } vs Disparagement of a
} } }
} } } } i am not on an ego trip, but it sure sounds
} like
} } } you
} } } } have some issues to work out. if you don't
} like
} } } what i
} } } } have to say, block my email address or simply
} } } delete
} } } } them. try to be a little more open minded.
} } } } john hoffpauir
} } } }
} } } }
} } } }
} } } } --- Matthias M�rgelin
} } } {Matthias.Morgelin-at-med.lu.se}
} } } } wrote:
} } } }
} } } } } ..."so I am loosing interest here"...etc
} } } } } blablabla.!!!
} } } } }
} } } } } This forum has not been established for
} persons
} } } like
} } } } } you to express your very own small personal
} } } ego-trip
} } } } } things all the time. Sometimes I really
} admire
} } } } } Nestor for his neverending positive attitude
} } } towards
} } } } } guys like you! Personally I would have
} blocked
} } } you
} } } } } much earler!
} } } } }
} } } } }
} } } } } ----- Original Message -----
} } } } } From: hoffpajo-at-yahoo.com
} } } } } Date: Wednesday, August 24, 2005 7:46 pm
} } } } } Subject: [Microscopy] Re: Discussion of
} high IQ
} } } vs
} } } } } Disparagement of a
} } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } }
} } } }
} } }
} }
}
-------------------------------------------------------------------
} } } } } } ---------
} } } } } } The Microscopy ListServer -- CoSponsor:
} The
} } } } } Microscopy Society of
} } } } } } AmericaTo Subscribe/Unsubscribe --
} } } } } }
} } } } }
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserverOn-Line
} } } } } Help
} } } } } }
} } } } }
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } } } } }
} } } } }
} } } }
} } }
} }
}
----------------------------------------------------------------
} } } } } }
} } } } } }
} } } } } }
} } } } } } --- Sergey Ryazantsev {sryazant-at-ucla.edu}
} } } wrote:
} } } } } }
} } } } } } } Dear John,
} } } } } } }
} } } } } } } Nestor has established a special filter
} to
} } } block
} } } } } all
} } } } } } } my messages not only
} } } } } } } to the ListServer but also emails
} addressed
} } } } } } } personally to him. He also
} } } } } } } denied me an explanation of what I did
} } } wrong.
} } } } } } } Therefore, I would like to
} } } } } } } ask the EM community to help me
} reinstate my
} } } } } name
} } } } } } } and membership at
} } } } } } } ListServer. If you feel comfortable, I
} } } would
} } } } } like
} } } } } } } to ask you to publish my
} } } } } } } letter on ListServer. I don't want to
} cause
} } } you
} } } } } any
} } } } } } } problems, so I suggest
} } } } } } } that if you decide to publish my letter,
} ask
} } } } } } } Nestor's permission first. I
} } } } } } } would also appreciate your opinion on
} this
} } } } } letter
} } } } } } } and would be happy to
} } } } } } } make corrections, clarifications, etc.
} } } Please
} } } } } feel
} } } } } } } free to tell me if this
} } } } } } } idea doesn't seem suitable and you would
} } } prefer
} } } } } not
} } } } } } } to participate. It's
} } } } } } } perfectly fine; I'll find other way to
} make
} } } my
} } } } } } } letter available to EM
} } } } } } } community. I would like to make it
} clear to
} } } my
} } } } } } } fellow microscopists that
} } } } } } } they may be treated in a similar way on
} } } } } ListServer
} } } } } } } if something is not
} } } } } } } done. For this reason, I want to speak
} } } directly
} } } } } to
} } } } } } } the people on the
} } } } } } } ListServer, not just Nestor. I also
} } } sincerely
} } } } } want
}
=== message truncated ===

____________________________________________________
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==============================Original Headers==============================
7, 20 -- From hoffpajo-at-yahoo.com Wed Aug 24 20:48:54 2005
7, 20 -- Received: from web50207.mail.yahoo.com (web50207.mail.yahoo.com [206.190.38.48])
7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j7P1msAs024388
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Dear Henrik,

There are several modifications for the JEOL-35, you need to know which
microscope you have. One type has a connection in the back, labeled as
JA-2, which is a 9-pin Amphenol connector. The other type has a fairly
large round connector for external beam control.

Look for diagrams in the book for wiring of that connector. I have diagram
for Amphenol connector only. Let me know if you need them.
Good luck,
Vlad

At 07:33 AM 8/23/2005 -0500, Henrik.Kaker-at-guest.arnes.si wrote:

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ok, I have finally had enough of the personal attacks
I have received. I will no longer be a member of this
listserver.
Those of you that have issues with me start your party
now.
John Hoffpauir

____________________________________________________
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Hi All,
We are trying to find someone to work on our Balzers 360M Freezed Fracture
machine. It is a "modernized" unit with a thin film monitor and rotary
shadow device. It was is good working order when it was "decommissioned"
about 4 years ago but has been sitting idle since then. We would like to
find
someone in the Sacramento/San Francisco area who can help us get this
intrument up and running and who we can call for future service. We would
also like to know about general reliability and parts availability.
Thanks,
Pat Kysar
UC Davis Medical Pathology
EM Lab
530-752-4701

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Dear Colleagues,

We have an old Phillips 501B SEM in the Physics department and they are
looking for a photomultiplier tube (XP 2010/XP 1010). So if you have one
or know someone who has one and willing to sell it, then please let us
know. You can contact me offline.

Thanks a lot.

Soumitra

*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dans-at-ameslab.gov) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, August 25, 2005 at 09:51:11
---------------------------------------------------------------------------

Email: dans-at-ameslab.gov
Name: Dan Shechtman

Organization: Ames Lab.

Title-Subject: [Filtered] Used Dimpling unit

Question: I am interested in purchasing a used Dimpling unit, VCR or Gatan.
Please send information including asking price, and if you can, a picture.

---------------------------------------------------------------------------

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I was concerned, as some you may have been, about the impact of the closure of the Walter Reed Medical Center in Washington, DC on the future of the Billings Microscope Collection housed in the National Musuem of Health and Medicine there. The museum director told me today that specific language in the BRAC closure order preserves the Museum facility. The museum's website is www.nmhm.washingtondc.museum

David Rothbard
US Bureau of Engraving and Printing

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Soumitra,

Try Alex Greene of Electron Optics Repair and Installation. He bought our
old Philips SEM 500 a few years ago, and may have its or other similar model
photomultiplier tubes available. Tho this info is a few years old, you may
be able to reach him at:

ablue-at-io.com

or: (512)282-5507

Gib
--
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-2785 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

} Dear Colleagues,
}
} We have an old Phillips 501B SEM in the Physics department and they are
} looking for a photomultiplier tube (XP 2010/XP 1010). So if you have one
} or know someone who has one and willing to sell it, then please let us
} know. You can contact me offline.
}
} Thanks a lot.
}
} Soumitra
}
} *************************************************************
} Soumitra Ghoshroy
} College Associate Professor, Biology
} Director, Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3268 (office), 646-3283 (lab)
} Fax: 505-646-3282

==============================Original Headers==============================
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The link works for me. Under Exhibits-Permanent there is a short note about the collection. There are some electron microscopes, but they were not well displayed when I was there last.

Ken Tiekotter {tiekotte-at-up.edu} wrote:

} David,
}
} Will the Billings collection have a website? The www.nmhm.....website states
} it is not in use.
}
} Regards,
} Ken
}
} _______________________________________
} Kenneth L. Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N Willamette Blvd.
} Portland, OR 97203 USA
}
} Tel.: 503.943.8861
} Email: tiekotte-at-up.edu
}
}
}
} On 8/25/05 11:11 AM, "rothbardd-at-netscape.net" {rothbardd-at-netscape.net}
} wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------------
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} }
} } I was concerned, as some you may have been, about the impact of the closure of
} } the Walter Reed Medical Center in Washington, DC on the future of the Billings
} } Microscope Collection housed in the National Musuem of Health and Medicine
} } there. �The museum director told me today that specific language in the BRAC
} } closure order preserves the Museum facility. The museum's website is
} } www.nmhm.washingtondc.museum
} }
} } David Rothbard
} } US Bureau of Engraving and Printing
} }
} } __________________________________________________________________
} } Switch to Netscape Internet Service.
} } As low as $9.95 a month -- Sign up today at http://isp.netscape.com/register
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Paul:

Mounting a digital SLR on a photo-port on a LM is very easy to
do - BUT I do not know of a digital SLR which uses a C-mount
specifically. The c-mounts are very small, digital SLR's, like film
SLR's use larger mounts like a nikon F-mount, Olympus O-mount,
Canon EF-mount, pentax KA-mount, or a more generic T-mount, or
other.

AND you will generally need to use some type of photo-eye
piece lens to project the image to the "film"/"sensor" plane of the
SLR

Your Leica may very well have an adapter on it for the C-
mounting which you will need to replace for a specific SLR body.

On 22 Aug 2005, at 17:56, pbarber-at-bu.edu wrote:

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} ---------------------------------------------------------------------------
}
} Email: pbarber-at-bu.edu
} Name: Paul Barber
}
} Organization: Boston University
}
} Title-Subject: [Filtered] MListserver:
}
} Question: I would like to be able to attach a digital SLR (ie Nikon D100) to a c-mount on a stereo microscope like a Leica MZ9.5 or Nikon SMZ800. I've heard conflicting answers from product reps, some saying that it isn't possible, some saying it is? However, I have no experience at all
microscopy, but need to buy a system that has at least some photodocumentation ability. I don't need (and can't afford) a 4-5000 dollar imaging system. It seems like putting a digital SLR on a c-mount would be a reasonable and less expensive way to do photodocumentation.
}
} Thanks
} Paul
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 12 -- From zaluzec-at-microscopy.com Mon Aug 22 17:54:00 2005
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} 7, 12 -- Subject: viaWWW: digital SLR to a c-mount on a stereo microscope
} 7, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

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Dear Listers,

Now that the whole world has gone digital, I just know there must be
bushels of those plastic TEM negative processing racks sitting around
feeling lonely and unwanted. We are starting a special program to
provide a home for these unfortunate victims of technological progress
and would be happy to take a few in.

If you have any of these to spare and would be willing to donate them
for the cost of shipping or a reasonable reimbursement, please let me
know. Thank you for caring.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu

==============================Original Headers==============================
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I recently purchased a used Molecular Dynamics Phosphor Imager. It was, of
course, sold "as is" and I have no idea if I will be able to use it. The
seller stated that it comes on, but the computer shuts down. I have no model
numbers or anything until it actually arrives and I can inspect it. I hope
to use the beast for both X-ray and autoradiography imaging, and perhaps,
X-ray diffraction powder camera work. If anyone on the list uses this brand
of equipment and might be willing to help me diagnose the problems with it,
please reply to me off line. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu

==============================Original Headers==============================
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3, 25 -- Subject: Phosphor Imager help
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (papalia-at-udel.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, August 25, 2005 at 14:12:04
---------------------------------------------------------------------------

Email: papalia-at-udel.edu
Name: John Papalia

Organization: U of Delaware

Title-Subject: [Filtered] TEM cryomicrotiming polymer sample

Question: Good afternoon,

I'm having trouble microtoming some polymer samples and was hoping for any suggestions or guidance that might be out.

My samples are thin films of Polystyrene/Polyethylenepropylene (hydrogenated polyisoprene) evaporatively cast fromm solvent to a thickness of 0.1-0.5 mm.

The problem - I've tried a vast number of different settings, cutting anywhere from -80C down to -140C, and the samples never truly get rigid, although the Tg of the rubber phase falls into the -70 to -90 range based on DSC testing. I basically can not get consistent sections thinner than 100nm, and I need samples at 80nm or thinner.

I have also tried embedding the samples, however this has not helped - the samples themselves stretch and deform even when embedded.

The equipment I have at my disposal is a Leica Ultracut UCT with EMFCS cryo attachment, a Diatome Static Line II, and a Diatome 35 degree cryo/dry diamond knife.

Thanks very much!!!
-John Papalia

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Does anyone know how the images from the toy laser pointers are made?
I've looked at them under a microscope and they look like digitized
images. Are they digital holograms or phase images? I'd like to know
because I'm curious and would like to know how I could make my own
projected image.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

==============================Original Headers==============================
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3, 25 -- Subject: Laser pointer images
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (aav_1972-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, August 25, 2005 at 19:27:55
---------------------------------------------------------------------------

Email: aav_1972-at-yahoo.com
Name: Arnold Villanueva

Education: Undergraduate College

Location: San Diego, California, USA

Question: Hello, I wanted to find out if there were ANY programs in ANY college and/or university that offer either Associate, Bachelors or Masters degrees in Microscopy. What about certificates or professional certifications in the field? Please let me know if you have any information. Thank you and have a nice day :)

---------------------------------------------------------------------------

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Paul,

I've been using a Nikon D100 onto Zeiss SV11 & Lumar stereomicroscopes
without any problem. No extra lens in the phototube was necessary, the
microscope itself projects the image onto the chip. The adapter was bought
from the company itself and though I've never tried it on a Leica nor
Olympus, I'm quite sure these companies also will be able to provide you the
correct adapters! Indeed a relatove cheap, but good solution for
photodocumentation!
Best regards,

Sven Terclavers

-------Original Message-------

X-from: edelmare-at-muohio.edu

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Paul:

Mounting a digital SLR on a photo-port on a LM is very easy to
do - BUT I do not know of a digital SLR which uses a C-mount
specifically. The c-mounts are very small, digital SLR's, like film
SLR's use larger mounts like a nikon F-mount, Olympus O-mount,
Canon EF-mount, pentax KA-mount, or a more generic T-mount, or
other.

AND you will generally need to use some type of photo-eye
piece lens to project the image to the "film"/"sensor" plane of the
SLR

Your Leica may very well have an adapter on it for the C-
mounting which you will need to replace for a specific SLR body.

On 22 Aug 2005, at 17:56, pbarber-at-bu.edu wrote:

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on Monday, August 22, 2005 at 11:05:10
}
---------------------------------------------------------------------------
}
} Email: pbarber-at-bu.edu
} Name: Paul Barber
}
} Organization: Boston University
}
} Title-Subject: [Filtered] MListserver:
}
} Question: I would like to be able to attach a digital SLR (ie Nikon D100)
to a c-mount on a stereo microscope like a Leica MZ9.5 or Nikon SMZ800. I've
heard conflicting answers from product reps, some saying that it isn't
possible, some saying it is? However, I have no experience at all
microscopy, but need to buy a system that has at least some
photodocumentation ability. I don't need (and can't afford) a 4-5000 dollar
imaging system. It seems like putting a digital SLR on a c-mount would be a
reasonable and less expensive way to do photodocumentation.
}
} Thanks
} Paul
}
}
---------------------------------------------------------------------------
}
} ==============================Original
Headers==============================
} 7, 12 -- From zaluzec-at-microscopy.com Mon Aug 22 17:54:00 2005
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} 7, 12 -- Subject: viaWWW: digital SLR to a c-mount on a stereo microscope
} 7, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of -
Headers==============================

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

==============================Original Headers==============================
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1, 28 -- From Sven.Terclavers-at-med.kuleuven.be Fri Aug 26 02:13:50 2005
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Hello-

I have a block copolymer sample (PS-PEO) that I need to cross-section with
the microtome, then stain to localize the PEO. Is there a stain that will
selectively attach to the PEO and not the PS? If so, which staining
method is better, en bloc or on the grid? The sample is in a thin (30-50
nm) layer on a wafer that I remove and embed in epoxy.

Thanks,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099
(408) 927-3856

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Leslie,

Instead of staining PEO segments, you can stain the PS
blocks much easier by using ruthenium acetate.

Wish this helps.

Susheng

--- lethomps-at-us.ibm.com wrote:

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}
} Hello-
}
} I have a block copolymer sample (PS-PEO) that I need
} to cross-section with
} the microtome, then stain to localize the PEO. Is
} there a stain that will
} selectively attach to the PEO and not the PS? If
} so, which staining
} method is better, en bloc or on the grid? The
} sample is in a thin (30-50
} nm) layer on a wafer that I remove and embed in
} epoxy.
}
} Thanks,
} Leslie
}
} Leslie Krupp (Thompson)
} IBM Almaden Research
} 650 Harry Road, K19/D2
} San Jose, CA 95120-6099
} (408) 927-3856
}

Susheng TAN, Ph.D
Department of Chemistry, Oklahoma State University
107 Physical Science
Stillwater, Oklahoma 74078 USA
Phone: (405)744-4835 Fax:(405)744-6007
eMail:tsushen-at-okstate.edu sstan33-at-yahoo.com

__________________________________
Yahoo! Mail for Mobile
Take Yahoo! Mail with you! Check email on your mobile phone.
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There is a great book on fractography of casting alloys by Gordon
Powell. I believe that the title is "Atlas of Casting Fractures" or
something along those lines. This book has fractographs for various cast
materials produced in the laboratory by various loading methods -
tensile, bend, impact, fatigue, etc. I am not sure if C355 is included,
but similar alloys will be.

The book is out of print, but can often be found at university libraries
or by interlibrary loan from your local public library. I saw a used
copy for sale on Amazon a couple of years ago. (Should have bought it,
but got a copy from the library.)

From my experience, it is not uncommon for fatigue fractures in cast
aluminum to not exhibit fatigue striations. Your description of a
dendritic appearance is a good characterization. Rubbing and smearing
are also not uncommon. Look for recessed areas where the surface is not
rubbed to see the true fracture features.

If the surface is smeared, it is smeared, and the fine fracture features
will be lost. Fatigue striations, especially fine striations from high
cycle fatigue are easily wiped out by smearing from crack closure if the
stress cycle is tension - compression. I don't think that using a higher
KV to penetrate through the smearing will be helpful. In fact, fine
striations are often more easily observed with low KV SE imaging, since
higher KV and BSE do not provide sufficient surface resolution to pick
up these fine features.

Hope this helps.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jean-ross-at-uiowa.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, August 26, 2005 at 09:47:21
---------------------------------------------------------------------------

Email: jean-ross-at-uiowa.edu
Name: Jean Ross

Organization: CMRF, University of Iowa

Title-Subject: [Filtered] MListserver:Photographic Equip. Available

Question: Hello everyone,

Our facility is getting out of the photo printing business and we have some equipment that we are willing to give to anyone who wants it and will pay for the shipping to their lab. One is an Ilford Ilfolab MG2650 B+W print processor and the other is a Durst Laborator 1200 point source enlarger. Both are in good working order and assorted spare parts and manuals are included. Email me directly at jean-ross-at-uiowa.edu if you are interested.

Jean Ross
Central Microscopy Research Facility
University of Iowa
85 EMRB
Iowa City, IA 52242
(319)335-8142

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}
} Hi all,
}
} our company sells adapters which connect all kind of digital consumer cameras, camcorders and SLR cameras to microscope c-mount couplers. The adapters we sell have an extra large lense, so that larger CCDs do not necessarily provide vignetting. We use a basic adapter which includes the magnification lense plus a camera ring which can be chosen individually for the camera.
}
} Everybody who is interested in a list of cameras which can be connected to a microscope may contact us.
}
} with best regards
}
} Anneliese Schmaus
}
} klughammer bio gmbh
} Strassbach 9
} 85229 Markt Indersdorf
}
} Tel. + 49 8136 6011
} Fax +49 8136 7098
}
} schmaus-at-klughammer.de
} www.klughammer.de
}

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Hi Guys,
I am new on the list and new to the vacuum technology field. I am looking
for a book that introduces the vacuum technology from experimental prospect
than just the theoretical one, what do you think the best book for this?

Thanks

Hany Ramadan

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Hany Ramadan wrote:
===========================================================
I am new on the list and new to the vacuum technology field. I am looking
for a book that introduces the vacuum technology from experimental prospect
than just the theoretical one, what do you think the best book for this?
===========================================================
In our own laboratory, we have found

Vacuum Methods in Electron Microscopy , Vol. 15 of the series Practical
Methods of Electron Microscopy by Prof. Wilber C. Bigelow

to be very helpful for understanding and solving problems involving vacuum
technology. And apparently so have others as well since it continues to be
a good seller even though it was originally published in 1994. See URL
http://www.2spi.com/catalog/books/book48.shtml This book can be
purchased via numerous other outlets, both on line and in certain university
book stores.

Disclaimer: SPI Supplies is a seller of the above mentioned text so we
might not be seen as an independent party relative to this posting.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================

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Hi Hany,

Here are four my favorite vacuum texts:

1. Good introductory book, minimal theory: "A User's Guide to Vacuum
Technology"

http://www.amazon.com/exec/obidos/tg/detail/-/0471270520/qid=1125198579/sr=2
-1/ref=pd_bbs_b_2_1/002-1507630-6605604?v=glance&s=books

2. Advanced and purely practical book on vacuum instrumentation:
"Experimental Innovations in Surface Science: A Guide to Practical
Laboratory Methods and Instruments"

http://www.amazon.com/exec/obidos/tg/detail/-/0387983325/ref=cm_aya_asin.tit
le/002-1507630-6605604?%5Fencoding=UTF8&v=glance

Two good books on vacuum materials, material treatments, and techniques. I
favor the #4, but both are good:

3. "Handbook of Materials and Techniques for Vacuum Devices (AVS Classics in
Vacuum Science and Technology)"

http://www.amazon.com/exec/obidos/tg/detail/-/1563963876/qid=1125199262/sr=1
-4/ref=sr_1_4/002-1507630-6605604?v=glance&s=books

4. "Handbook of Electron Tube and Vacuum Techniques (American Vacuum Society
Classics)"

http://www.amazon.com/exec/obidos/tg/detail/-/1563961210/ref=pd_sim_b_1/002-
1507630-6605604?%5Fencoding=UTF8&v=glance

Enjoy!

Valery Ray
---------------------
Particle Beam Systems
& Technology

-----Original Message-----
X-from: ramadanhany-at-gmail.com [mailto:ramadanhany-at-gmail.com]
Sent: Saturday, August 27, 2005 12:07 AM
To: vray-at-partbeamsystech.com

Hi Guys,
I am new on the list and new to the vacuum technology field. I am looking
for a book that introduces the vacuum technology from experimental prospect
than just the theoretical one, what do you think the best book for this?

Thanks

Hany Ramadan

==============================Original Headers==============================
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I'm looking for TEM or SEM material (specimens
and/or films) of West Nile Virus.

If anyone has these or sub-units, let me know.
I will discuss transfer off-line.

gary g.

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In my opinion, the most useful book is: "Experimental innovations in Surface Science" by John T. Yates, Jr.

This book has a strong experimental side to it and presents many examples of vacuum technology with plenty of schematics and illustrations. It comes very handy when one wishes to either design systems or work on a vacuum system for the first time.

Regards,

Olivier

Olivier Guise, Ph.D.

GE Industrial - Plastics
Selkirk Technology Bldg
One Noryl Avenue
Selkirk, NY 12158

P: 518-475-5463
F: 518-475-5856
D: *233-5463
E: olivier.guise-at-ge.com
www.geplastics.com

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I am trying to investigate the cross-section of a thick (~1mm) metal substrate coated with a very thin (~1um) silane film. My attempts to prepare a clean cross-section have been unsuccesful and i have not been able to distinguish the thin film from the substrate. So i am wondering if anyone has advice on the best way to prepare a cross-section of this type of sample. Any ideas? Suggestions?

Olivier Guise, Ph.D.

GE Industrial - Plastics
Selkirk Technology Bldg
One Noryl Avenue
Selkirk, NY 12158

P: 518-475-5463
F: 518-475-5856
D: *233-5463
E: olivier.guise-at-ge.com
www.geplastics.com

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I've taught a Vacuum Science and Technology course several times. I've
used a couple of books and other references.

The bible, as far as I am concerned, is A. Roth's "Vacuum Technology"
published by North-Holland. I don't know what the latest version is.
This has the all the information both theoretical and practical. The
next best book is John O'Hanlon's "A User's Guide to Vacuum Technology"
published by Wiley. I have the 1st and 2nd edition of this book, but I
don't know whether a newer version is out. I also have Chambers, Fitch,
and Halliday's "Basic Vacuum Technology", but I don't recommend it. One
of the best sources of practical vacuum instruction comes from some
vendors' catalog. I haven't seen one for some time, but the
Leybold-Heraus catalog had a full vacuum science and technolgoy section
in it. Now it is just Leybold Vacuum and you can order the CD with
their product line and a CD with the "Fundamentals of Vacuum Technology"
from them for free after you register.
http://www.leybold.com/index.php?id=257&L=1

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: ramadanhany-at-gmail.com [mailto:ramadanhany-at-gmail.com]
Sent: Friday, August 26, 2005 9:13 PM
To: Walck-at-SouthBayTech.com

Hi Guys,
I am new on the list and new to the vacuum technology field. I am
looking for a book that introduces the vacuum technology from
experimental prospect than just the theoretical one, what do you think
the best book for this?

Thanks

Hany Ramadan

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Hi
I would strongly recommend Wil Bigelow's book.
It's totally relevant to EM work, it's full of both practical
and theoretical stuff, and it's so well-written that it's a
pleasure to read.

cheers

rtch

--
Ritchie Sims Ph D Phone
: 64 9 3737599 ext 87713
Microanalyst Fax :
64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand

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Group,

Do any of you who run electron microprobe or SEM with EDS analysis, know of any software that can take EDS data calculated as oxide weight percents and generate a chemical formula and a possible mineral ID.

Thanks

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (t.keller-at-uni-jena.de) from
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
Monday, August 29, 2005 at 04:34:22
---------------------------------------------------------------------------

Email: t.keller-at-uni-jena.de
Name: Thomas Keller

Organization: Friedrich-Schiller-University Jena

Title-Subject: [Filtered] Cryo TEM holder Jeol EM31100

Question: Dear all,

currently I am trying to operate our double-tilt cryo TEM sample
holder Jeol EM31100. Recently there is a Cryo leakage, and also, the
see-saw is loosened from the fixing springs when operated in the TEM.

Is there anybody out there with experience - we want to fix these
issues in our lab. The holder needs to be siginifcantly unmounted and
therefore we are seeking more detailed information.

Best regards,
Thomas

---------------------------------------------------
Dr. Thomas Keller
Institute of Materials Science and Technology (IMT)
Friedrich-Schiller-University Jena
D-07743 Jena

---------------------------------------------------------------------------

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (nair.ashwin-at-gmail.com) from
http://www.microscopy.com/MLFormMail.html on Monday, August 29, 2005
at 17:16:10
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Email: nair.ashwin-at-gmail.com
Name: Ashwin Nair

Organization: University of Texasat Arlington

Title-Subject: [Filtered] MListserver:

Question: Hi:

I have a Messer Glas Knife cutter in my lab and do not know how to
use it... It would be great if someone could help me find a user
manual for it... I am a student and don't want to mess with the
instrument though I don't mind learning by trial and error...the
instrument is made by Sunkay Laboratory, Tokyo...
Can somebody please help me...

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (donnarl-at-gmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
August 29, 2005 at 17:44:07
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Email: donnarl-at-gmail.com
Name: Changhui

Title-Subject: [Filtered] translation control

Question: Dear Friends,

The left tranlation control (x) of the stage of our CM12 looks out of
control partially. The left translation control ear (opposite to the
holder loading) can move the stage in/out about 2mm. It can not move
the stage fully. After the certain position, I can still move the
mechnical lever, but the stage does not move. If I hold or press the
holder to the column, I gain the control again.

Does anyone have any idea on how to fix the problem?

Thanks!

Changhui

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Ashwin Nair wrote:
==========================================================
I have a Messer Glas Knife cutter in my lab and do not know how to
use it... It would be great if someone could help me find a user
manual for it... I am a student and don't want to mess with the
instrument though I don't mind learning by trial and error...the
instrument is made by Sunkay Laboratory, Tokyo...
Can somebody please help me...
==========================================================
SPI Supplies has been the distributor for Sunkay Laboratories for some
years. We could probably help you if you sent us the serial number on the
back of the unit that you have in your possession. You will find some
information on URL
http://www.2spi.com/catalog/knives/glass.shtml
which might be of further assistance.

Disclaimer: SPI Supplies is a distributor of the Sunkey Laboratories Messer
Glass Knife Maker.

Chuck

PS: Remember that we are 100% paperless and the only way we can keep track
of this kind of correspondence is if you reply using the reply feature of
your e-mail software. That way the entire string of correspondence will be
in one place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================

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One possibility. On the CM100, the linkage is fastened wth 2 hex screws
near the x-translation indicator above and below the universal joint. If
they are loose, the stage will fail to move. I think the CM12 may be
similar and it could be a simple fix. Good luck.

--
Pang (Wai Pang Chan, wpchan-at-u.washington.edu)

On Tue, 30 Aug 2005 donnarl-at-gmail.com wrote:

} Email: donnarl-at-gmail.com
} Name: Changhui
}
} Title-Subject: [Filtered] translation control
}
} Question: Dear Friends,
}
} The left tranlation control (x) of the stage of our CM12 looks out of
} control partially. The left translation control ear (opposite to the
} holder loading) can move the stage in/out about 2mm. It can not move
} the stage fully. After the certain position, I can still move the
} mechnical lever, but the stage does not move. If I hold or press the
} holder to the column, I gain the control again.

==============================Original Headers==============================
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Leslie,

Here is a response to your question from my friendly local staining expert:

{ { {
My immediate response would be RuO4, although I don't know the relative
reaction rates of the PS and PEO off hand. The basic question here seems to
be is it better to stain and then section or section and then stain?

This depends on how easy the system is to section in the first place. If it
cuts easily, then I'd section and then stain since the problem with the
alternative is controlling the extent of reaction - you get an unstained
interior, a surface layer that completely destroyed and, somewhere in
between, a region that's just right.
} } }

Susheng,

Do you have a reference or two to Ruthenium Acetate staining?

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------

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Dear everyone,

We are interested in buying a relatively inexpensive ($200-300) flatbed
scanner to scan EM negatives (31/4 x 4") for my EM class. If anyone knows
of a good scanner that comes with medium format film holders and does a
decent scanning job, then please let me know.

Thanks in advance,

Soumitra

*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (j.compan-at-fz-juelich.de) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 30, 2005 at 11:25:21
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Email: j.compan-at-fz-juelich.de
Name: Compan jeremie

Organization: FZJ

Education: Graduate College

Location: Juelich, Germany

Question: Hi,
I would like to know how to determine a extinction angle under
polarized light!!
I am looking on internet and I do not find any clear answer.
Please can you help me!!

Thanks in advance

Jeremie

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Jeremie,
Between crossed polars, when the optical axis of a
birefringent element (a crystal domain) in your sample is parallel to
either the analyzer or the polarizer, that element will not affect
the state of polarization and the field will be dark, as if there
were no sample there at all. So to measure this, you need a rotatable
stage and you need a way to define the axis of your sample to the
axis of the analyzer and polarizer. For example, if you mount an edge
of your sample parallel to the microscope slide. If both polarizer
and analyzer can rotate on you microscope, then you also need to make
sure that you know what direction their transmission axes are
relative to the stage. With these, you can rotate the stage, say in 5
degree increments and look at (record) the intensity of your sample.
When it is maximally dark, the sample is at extinction, and the angle
between the reference direction on your sample and the transmission
axis of the polarizer is the angle of extinction.

Hope this helps,
Tobias
}
} Email: j.compan-at-fz-juelich.de
} Name: Compan jeremie
}
} Organization: FZJ
}
} Education: Graduate College
}
} Location: Juelich, Germany
}
} Question: Hi,
} I would like to know how to determine a extinction angle under
} polarized light!!
} I am looking on internet and I do not find any clear answer.
} Please can you help me!!
}
} Thanks in advance
}
} Jeremie
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

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I would like to make a video about the process of
mitosis for my school students, what kind of process
would be the most appropiate to use and wich kind of
cells could I use?

Thanks

H.Ibarra
Studium Institute

__________________________________________________
Correo Yahoo!
Espacio para todos tus mensajes, antivirus y antispam �gratis!
Reg�strate ya - http://correo.espanol.yahoo.com/

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Oliver:

Sample preparation for polymeric films on metallic substrates takes some
practice and patience. If you can prepare good samples of polymer and
metals separately, you should be able to work a procedure to get this
sample adequately prepared.

Coatings at 1um and below are much harder to get consistent reliable
results than for thicker coatings. One possibility to explain your
difficulties is that the coating is thinner than you expect. If you have
good confidence in your sample preparation, you might want to use
another method to check the approximate thickness. We have struggled to
prepare cross sections fo samples only to find that the coating
thickness was really less than 100 angstroms.

If the sample will tolerate compression mounting, you can get good
contrast between the coating and mounting material by wrapping the
sample with aluminum foil. The compression during molding will press the
foil tight against the samples surface and retain the coating during
polishing. Clamping the sample against a flat, conforming backing will
achieve similar but sometimes less satisfactory results if you are using
castable mounting.

Use low nap polishing cloths to avoid rounding during polishing. Beyond
that it may require trial and error of the process to find what works
for this particular substrate and coating material combination.

} I am trying to investigate the cross-section of a thick (~1mm) metal substrate coated with a very thin (~1um) silane film. My attempts to prepare a clean cross-section have been unsuccesful and i have not been able to distinguish the thin film from the substrate. So i am wondering if anyone has advice on the best way to prepare a cross-section of this type of sample. Any ideas? Suggestions?
}
}
} Olivier Guise, Ph.D.
}
} GE Industrial - Plastics
} Selkirk Technology Bldg
} One Noryl Avenue
} Selkirk, NY 12158
}
} P: 518-475-5463
} F: 518-475-5856
} D: *233-5463
} E: olivier.guise-at-ge.com
} www.geplastics.com
}
}
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870

==============================Original Headers==============================
7, 23 -- From hanke-at-mee-inc.com Tue Aug 30 21:00:08 2005
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Hi,
The problem could be in sample holder. There is a slot on the holder that
fits to a guide pin on the goniometer. If there is any dirt, the movement
of holder can be blocked by it. Please, check the slot on the holder for
the dirt. You should also check the width of the slot. The holder has to
move smoothly but without any play on the guide pin.
The precise description of the slot adjustment can be found in the �Single
Tilt Holder Handbook.�
Hopping it helps. Best regards Oldrich
-------------------------------------------
Oldrich Benada
EM lab.
Inst. Microbiology,
Acad. Sci. CR
Czech Republic

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} August 29, 2005 at 17:44:07
} ---------------------------------------------------------------------------
}
} Email: donnarl-at-gmail.com
} Name: Changhui
}
} Title-Subject: [Filtered] translation control
}
} Question: Dear Friends,
}
} The left tranlation control (x) of the stage of our CM12 looks out of
} control partially. The left translation control ear (opposite to the
} holder loading) can move the stage in/out about 2mm. It can not move
} the stage fully. After the certain position, I can still move the
} mechnical lever, but the stage does not move. If I hold or press the
} holder to the column, I gain the control again.
}
} Does anyone have any idea on how to fix the problem?
}
} Thanks!
}
} Changhui

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4, 25 -- From benada-at-biomed.cas.cz Wed Aug 31 02:15:29 2005
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (nmg944-at-uow.edu.au) from
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on Wednesday, August 31, 2005 at 03:16:32
---------------------------------------------------------------------------

Email: nmg944-at-uow.edu.au
Name: Nicole Grant

Organization: University of Wollongong, Australia

Education: Graduate College

Location: Wollongong, NSW, Australia

Question: I have stained some fixed sections (1.25% Glut 4.00%
Paraformaldehyde + PO4 buffer)of lotus (Nulembo nucifera)recepticle
tissue then set in Glycol Methacrylate(GMA) resin. Sectioned slides
were stained with PAS/TBO (periodic acid Schiff's reagent and
Toluidine Blue O). The slides show a number of sections of certain
tissue cells stained green. Do you know what is shown by the green
stain? Green shows up only in epidermal cells on some parts of the
recepticle and it is evenly distributed in other parts. It was
thought that the green could be indicating lipids? Please help. Thanks
Nicole

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Below is the result of your feedback form
(NJZFM-ultra-55). It was submitted by
(t.keller-at-uni-jena.de) from
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Email: t.keller-at-uni-jena.de
Name: Thomas Keller

Organization: Friedrich-Schiller-University Jena

Title-Subject: [Filtered] staining cellulose fibers with silver sulfide

Question: Dear all,

I am looking for a protokol to stain cellulose fibers with silver sulfide.

Any help or link is appreciated.

Thanks Thomas

---------------------------------------------------
Dr. Thomas Keller
Institute of Materials Science and Technology (IMT)
Friedrich-Schiller-University Jena
L�bdergraben 32
D-07743 Jena
Germany
Phone: ++ 49 3641 947742
Fax: ++ 49 3641 947732
Mobile: ++ 49 170 1439522
Internet: t.keller-at-uni-jena.de
---------------------------------------------------
Visit us under http://www.uni-jena.de/matwi/

"Making Materials Science Work 4 U"
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Listers,

Here is the September 2005 Microscopy Today table of contents. I will
close the subscription list for this issue on Wednesday September 7, 2005.

Microscopists in North America and MSA members anywhere may have free
subscriptions. Anyone else may subscribe for US$35 per year (to
PARTIALLY cover postage). All subscriptions at
http://www.microscopy-today.com Thank you. Foreign rates will increase
for 2006.

Ron Anderson, Editor
==================================

Getting the Green Light to Measure Small Forces
Stephen W. Carmichael, Mayo Clinic

The Application of Electron Microscopy Techniques to the Space Shuttle
Columbia Accident Investigation
Sandeep Shah and Greg Jerman, Materials Diagnostics Team, NASA Marshall
Space Flight Center

Integrating Light and TEM Information with F-TEM images
P.A. Sims, C.A. Lockwood and J.D. Hardin, Zoology Department, University
of Wisconsin

Fluctuation Microscopy: What is it?
Michael M. J. Treacy, Dept. of Physics and Astronomy, Arizona State
University

TEM of Paraffin-Embedded H&E-Stained Sections for Viral Diagnosis (An
Unusual Papovavirus Case)
Januario C. Estrada, M. Angelica Selim, and Sara E. Miller, Duke
University Medical Center

Energy-Filtered Electron Holography
Rodney A. Herring, University of Victoria, Victoria, BC Canada

Imaging of the Development and Therapeutic Response of an In Vivo Fungal
Catheter Biofilm
Jeniel Nett and David Andes, Department of Medicine, Section of
Infectious Diseases, University of Wisconsin

Eucentric Stage for Recording Stereo Pair Photomacrographs
Ted Clarke, Metallurgical Failure Analysis Consultant

A Novel FIB Method for Preparing Three Dimensional TEM Specimens
Nathan Wang, Cypress Semiconductor Corp., San Jose, CA

ASTM Standards in Microscopy
John J. Friel, Chairman of ASTM Committee E04.11on X-ray and Electron
Metallography

Microscopy in Costa Rica
Alwyn Eades, Lehigh University, Bethlehem, PA

A Stable Lead Citrate Stain for Grids
Leona Cohen-Gould, Weill Medical College of Cornell University

New and Interesting at M&M-2005

Industry News

NetNotes

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