I'm hoping someone on the list has some expertise that they could share with me. I've searched the list archives but didn't turn up anything. I'm working with some thick sections (about 1 mm) embedded in Spurr's low viscosity epoxy resin. The goal is to incubate these sections with fluorescently labelled nucleic acid probes. The first thing I plan to do is try incubating these with a Polysciences epoxy removal kit to make the cells accessible for staining. I'm wondering if anyone has experience with using nucleic acid probes on thin sections, or could direct me to some good references. Is there anything I could use to increase the permeability of the epoxy, or are my probes just going to bind to whatever is exposed with the epoxy removal solution? Also, could anyone recommend a way to fix the sections on coverslips for manipulation in the staining procedure and later viewing with epifluorescence?
Here's one for the "never a dull moment" category. I have occasionally used the freeze-drying effect of our FESEM's cryostage to sublime ice off of LN2 plunge-frozen samples. Now I have someone who would like to try this with liquids such as benzene and other solvents. I'm willing to give it a shot, but have no clue how such liquids behave at low temperatures. Our client says the solvents will freeze at the temperatures we operate our stage at, but she also has no idea whether "freeze drying" will occur with them.
Has anyone tried this? As usual, I'll be checking the literature, but personal experiences are always the most useful, and I figure you folks have collectively done it all at some point.
Thanks for all the help----past, present, and future.
Cheers, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 10, 27 -- From TindallR-at-missouri.edu Fri Sep 2 09:22:08 2005 10, 27 -- Received: from um-proofpoint2.um.umsystem.edu (um-proofpoint2.umsystem.edu [207.160.151.136]) 10, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82EM6an026461 10, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 09:22:07 -0500 10, 27 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 10, 27 -- by um-proofpoint2.um.umsystem.edu (8.13.3/8.13.3) with ESMTP id j82EKFLn010992 10, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 09:21:53 -0500 10, 27 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 10, 27 -- Fri, 2 Sep 2005 09:19:56 -0500 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 10, 27 -- Content-class: urn:content-classes:message 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; 10, 27 -- charset="us-ascii" 10, 27 -- Subject: Cryo SEM: Freeze drying question 10, 27 -- Date: Fri, 2 Sep 2005 09:19:55 -0500 10, 27 -- Message-ID: {BA876152E8653240BE8572E897083EE79803C1-at-UM-EMAIL09.um.umsystem.edu} 10, 27 -- X-MS-Has-Attach: 10, 27 -- X-MS-TNEF-Correlator: 10, 27 -- Thread-Topic: Cryo SEM: Freeze drying question 10, 27 -- Thread-Index: AcWvyWPprjZOorSJSKWVU9++Jz1lzA== 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 27 -- To: {microscopy-at-microscopy.com} 10, 27 -- X-OriginalArrivalTime: 02 Sep 2005 14:19:56.0004 (UTC) FILETIME=[648AEA40:01C5AFC9] 10, 27 -- X-Proofpoint-Spam-Details: rule=tagforward_notspam policy=tagforward score=0 mlx=0 adultscore=0 adjust=0 reason=mlx engine=3.0.0-05090100 definitions=3.0.0-05090111 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j82EM6an026461 ==============================End of - Headers==============================
I assume that in checking the literature you will be determining the temperature and pressure at which each organic will sublime and the possibility of contaminating the chamber and other lower parts of the column. I assume that you are using an anticontaminator around/above the specimen holder; have you every lost control of the temperature or otherwise have the specimen "warm" up to room temp under the beam? Working with frozen organic solvents is something that I would contact your SEM manufacturer about and get their input.
I also wonder why you are getting ice on your samples. Are you using a device and method of avoiding ice contamination? Do you use LN2 slush? Having worked also with liquid helium 4 to freeze biological specimens, ice contamination was not an option so it can be done.
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Friday, September 02, 2005 9:22 AM To: neuberger1234-at-comcast.net
Dear Listers,
Here's one for the "never a dull moment" category. I have occasionally used the freeze-drying effect of our FESEM's cryostage to sublime ice off of LN2 plunge-frozen samples. Now I have someone who would like to try this with liquids such as benzene and other solvents. I'm willing to give it a shot, but have no clue how such liquids behave at low temperatures. Our client says the solvents will freeze at the temperatures we operate our stage at, but she also has no idea whether "freeze drying" will occur with them.
Has anyone tried this? As usual, I'll be checking the literature, but personal experiences are always the most useful, and I figure you folks have collectively done it all at some point.
Thanks for all the help----past, present, and future.
Cheers, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 19, 23 -- From neuberger1234-at-comcast.net Fri Sep 2 10:16:24 2005 19, 23 -- Received: from rwcrmhc12.comcast.net (rwcrmhc12.comcast.net [216.148.227.85]) 19, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82FGOkr002488 19, 23 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 10:16:24 -0500 19, 23 -- Received: from personal73sg05 (c-67-167-236-68.hsd1.il.comcast.net[67.167.236.68]) 19, 23 -- by comcast.net (rwcrmhc12) with SMTP 19, 23 -- id {2005090215162301400b0cnse} ; Fri, 2 Sep 2005 15:16:23 +0000 19, 23 -- From: "Damian Neuberger" {neuberger1234-at-comcast.net} 19, 23 -- To: "Microscopy-at-Microscopy. Com" {microscopy-at-microscopy.com} , 19, 23 -- {TindallR-at-missouri.edu} 19, 23 -- Subject: RE: [Microscopy] Cryo SEM: Freeze drying question 19, 23 -- Date: Fri, 2 Sep 2005 10:16:21 -0500 19, 23 -- Message-ID: {OKEJIDCNBDPPLLHANALIGEJHDAAA.neuberger1234-at-comcast.net} 19, 23 -- MIME-Version: 1.0 19, 23 -- Content-Type: text/plain; 19, 23 -- charset="iso-8859-1" 19, 23 -- Content-Transfer-Encoding: 7bit 19, 23 -- X-Priority: 3 (Normal) 19, 23 -- X-MSMail-Priority: Normal 19, 23 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.6604 (9.0.2911.0) 19, 23 -- Importance: Normal 19, 23 -- In-Reply-To: {200509021422.j82EMHNx026601-at-ns.microscopy.com} 19, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
A follow-up to my first question: I've just been reminded of the obvious hazard of solvent fumes, although the amounts involved would be quite small. Does anyone know if the mist traps on rotary pumps would be effective at trapping these? Our RPs are, unfortunately, in the same room as the scope.
Thanks, Randy
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Friday, September 02, 2005 9:25 AM To: Tindall, Randy D.
Dear Listers,
Here's one for the "never a dull moment" category. I have occasionally used the freeze-drying effect of our FESEM's cryostage to sublime ice off of LN2 plunge-frozen samples. Now I have someone who would like to try this with liquids such as benzene and other solvents. I'm willing to give it a shot, but have no clue how such liquids behave at low temperatures. Our client says the solvents will freeze at the temperatures we operate our stage at, but she also has no idea whether "freeze drying" will occur with them.
Has anyone tried this? As usual, I'll be checking the literature, but personal experiences are always the most useful, and I figure you folks have collectively done it all at some point.
Thanks for all the help----past, present, and future.
Cheers, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 10, 27 -- From TindallR-at-missouri.edu Fri Sep 2 09:22:08 2005 10, 27 -- Received: from um-proofpoint2.um.umsystem.edu (um-proofpoint2.umsystem.edu [207.160.151.136]) 10, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82EM6an026461 10, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 09:22:07 -0500 10, 27 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 10, 27 -- by um-proofpoint2.um.umsystem.edu (8.13.3/8.13.3) with ESMTP id j82EKFLn010992 10, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 09:21:53 -0500 10, 27 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 10, 27 -- Fri, 2 Sep 2005 09:19:56 -0500 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 10, 27 -- Content-class: urn:content-classes:message 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; 10, 27 -- charset="us-ascii" 10, 27 -- Subject: Cryo SEM: Freeze drying question 10, 27 -- Date: Fri, 2 Sep 2005 09:19:55 -0500 10, 27 -- Message-ID: {BA876152E8653240BE8572E897083EE79803C1-at-UM-EMAIL09.um.umsystem.edu} 10, 27 -- X-MS-Has-Attach: 10, 27 -- X-MS-TNEF-Correlator: 10, 27 -- Thread-Topic: Cryo SEM: Freeze drying question 10, 27 -- Thread-Index: AcWvyWPprjZOorSJSKWVU9++Jz1lzA== 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 27 -- To: {microscopy-at-microscopy.com} 10, 27 -- X-OriginalArrivalTime: 02 Sep 2005 14:19:56.0004 (UTC) FILETIME=[648AEA40:01C5AFC9] 10, 27 -- X-Proofpoint-Spam-Details: rule=tagforward_notspam policy=tagforward score=0 mlx=0 adultscore=0 adjust=0 reason=mlx engine=3.0.0-05090100 definitions=3.0.0-05090111 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j82EM6an026461 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 27 -- From TindallR-at-missouri.edu Fri Sep 2 10:17:45 2005 18, 27 -- Received: from um-proofpoint2.um.umsystem.edu (um-proofpoint2.umsystem.edu [207.160.151.136]) 18, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82FHjRT005334 18, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 10:17:45 -0500 18, 27 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 18, 27 -- by um-proofpoint2.um.umsystem.edu (8.13.3/8.13.3) with ESMTP id j82FGuC2003808 18, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 10:17:39 -0500 18, 27 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 18, 27 -- Fri, 2 Sep 2005 10:17:30 -0500 18, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 18, 27 -- Content-class: urn:content-classes:message 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain; 18, 27 -- charset="us-ascii" 18, 27 -- Subject: FW: [Microscopy] Cryo SEM: Freeze drying question 18, 27 -- Date: Fri, 2 Sep 2005 10:17:30 -0500 18, 27 -- Message-ID: {BA876152E8653240BE8572E897083EE79803C3-at-UM-EMAIL09.um.umsystem.edu} 18, 27 -- X-MS-Has-Attach: 18, 27 -- X-MS-TNEF-Correlator: 18, 27 -- Thread-Topic: [Microscopy] Cryo SEM: Freeze drying question 18, 27 -- Thread-Index: AcWvyhaDbdU0Aga2TZKDbbYkbMDzFgABxySg 18, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 18, 27 -- To: {microscopy-at-microscopy.com} 18, 27 -- X-OriginalArrivalTime: 02 Sep 2005 15:17:30.0581 (UTC) FILETIME=[6FA18850:01C5AFD1] 18, 27 -- X-Proofpoint-Spam-Details: rule=tagforward_notspam policy=tagforward score=0 mlx=0 adultscore=0 adjust=0 reason=mlx engine=3.0.0-05090100 definitions=3.0.0-05090111 18, 27 -- Content-Transfer-Encoding: 8bit 18, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j82FHjRT005334 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mmckilli-at-smurfit.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 2, 2005 at 08:32:34 ---------------------------------------------------------------------------
Question: We recently purchased a Nikon digital camera for optical microscopes as well as Image Pro image analysis software.
We have abandoned use of Image Pro software because of contant problems. Does anyone have recommendations for comparable image analysis software packages?
In a message dated 9/2/05 12:27:37 PM, mmckilli-at-smurfit.com writes:
} We have abandoned use of Image Pro software because of contant } problems. Does anyone have recommendations for comparable image } analysis software packages?
What kind of problems? Is there some kind of processing or measurement you need that is not provided, or is the software crashing, or do you just not understand how to use it? Image Pro Plus is a widely used and generally rather powerful package. Certainly there are others, including ones that are "comparable." But they may present "comparable" problems for your application, too.
==============================Original Headers============================== 5, 17 -- From DrJohnRuss-at-aol.com Fri Sep 2 11:41:04 2005 5, 17 -- Received: from imo-m26.mx.aol.com (imo-m26.mx.aol.com [64.12.137.7]) 5, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82Gf4Dw026886 5, 17 -- for {microscopy-at-ns.microscopy.com} ; Fri, 2 Sep 2005 11:41:04 -0500 5, 17 -- Received: from DrJohnRuss-at-aol.com 5, 17 -- by imo-m26.mx.aol.com (mail_out_v38_r4.1.) id v.c8.6660c2a6 (4320) 5, 17 -- for {microscopy-at-sparc5.microscopy.com} ; Fri, 2 Sep 2005 12:40:56 -0400 (EDT) 5, 17 -- From: DrJohnRuss-at-aol.com 5, 17 -- Message-ID: {c8.6660c2a6.3049da98-at-aol.com} 5, 17 -- Date: Fri, 2 Sep 2005 12:40:56 EDT 5, 17 -- Subject: Re: [Microscopy] viaWWW: Image analysis software 5, 17 -- To: microscopy-at-ns.microscopy.com 5, 17 -- MIME-Version: 1.0 5, 17 -- Content-Type: text/plain; charset="US-ASCII" 5, 17 -- Content-Transfer-Encoding: 7bit 5, 17 -- X-Mailer: AOL 5.0 for Mac sub 28 5, 17 -- X-Spam-Flag: NO ==============================End of - Headers==============================
Could you advise me on the best primary fixative to use for marine species that need both light microscopy and TEM? Currently we are fixing our specimens in 2.5%Glut/Form in cacodylate buffer and bisecting the animal -half for em and half for light microscopy. We perform histology on the LM half first, then if we need to retrieve the em fixed tissue we have the ability. In an effort to reduce work load and tissue storage do you have other suggestions?
Sue Tyler Dept. of Commerce Cooperative Oxford Laboratory Oxford, MD. sue.tyler-at-noaa.gov
==============================Original Headers============================== 2, 18 -- From sue.tyler-at-noaa.gov Fri Sep 2 12:43:04 2005 2, 18 -- Received: from hermes.nos.noaa.gov (hermes.nos.noaa.gov [140.90.119.204]) 2, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82Hh4Lu003015 2, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 12:43:04 -0500 2, 18 -- Received: from [10.60.12.125] ([10.60.12.125]) by 2, 18 -- hermes.nos.noaa.gov (Netscape Messaging Server 4.15) with ESMTP 2, 18 -- id IM7AJM00.SDS for {Microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 2, 18 -- 13:42:58 -0400 2, 18 -- Message-ID: {43188F22.3010105-at-noaa.gov} 2, 18 -- Date: Fri, 02 Sep 2005 13:42:58 -0400 2, 18 -- From: "Sue Tyler" {Sue.Tyler-at-noaa.gov} 2, 18 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 2, 18 -- X-Accept-Language: en-us, en 2, 18 -- MIME-Version: 1.0 2, 18 -- To: Microscopy-at-microscopy.com 2, 18 -- Subject: TEM best fixative question 2, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Randy, Have a look at http://www.sisweb.com/referenc/applnote/app-84.htm
I found this application note related to your follow-up question while looking for a new mist filter for our mechanical pump. It seems that normal oil mist filters are only effective at trapping the high molecular weight hydrocarbons from pump oil.
Steve Szewczyk ORISE Contractor, US Army Research Lab
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Friday, September 02, 2005 11:20 AM To: Szewczyk, Steven (Cont, ARL/WMRD)
A follow-up to my first question: I've just been reminded of the obvious hazard of solvent fumes, although the amounts involved would be quite small. Does anyone know if the mist traps on rotary pumps would be effective at trapping these? Our RPs are, unfortunately, in the same room as the scope.
Thanks, Randy
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Friday, September 02, 2005 9:25 AM To: Tindall, Randy D.
Dear Listers,
Here's one for the "never a dull moment" category. I have occasionally used the freeze-drying effect of our FESEM's cryostage to sublime ice off of LN2 plunge-frozen samples. Now I have someone who would like to try this with liquids such as benzene and other solvents. I'm willing to give it a shot, but have no clue how such liquids behave at low temperatures. Our client says the solvents will freeze at the temperatures we operate our stage at, but she also has no idea whether "freeze drying" will occur with them.
Has anyone tried this? As usual, I'll be checking the literature, but personal experiences are always the most useful, and I figure you folks have collectively done it all at some point.
Thanks for all the help----past, present, and future.
Cheers, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 10, 27 -- From TindallR-at-missouri.edu Fri Sep 2 09:22:08 2005 10, 27 -- Received: from um-proofpoint2.um.umsystem.edu (um-proofpoint2.umsystem.edu [207.160.151.136]) 10, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82EM6an026461 10, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 09:22:07 -0500 10, 27 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 10, 27 -- by um-proofpoint2.um.umsystem.edu (8.13.3/8.13.3) with ESMTP id j82EKFLn010992 10, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 09:21:53 -0500 10, 27 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 10, 27 -- Fri, 2 Sep 2005 09:19:56 -0500 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 10, 27 -- Content-class: urn:content-classes:message 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; 10, 27 -- charset="us-ascii" 10, 27 -- Subject: Cryo SEM: Freeze drying question 10, 27 -- Date: Fri, 2 Sep 2005 09:19:55 -0500 10, 27 -- Message-ID: {BA876152E8653240BE8572E897083EE79803C1-at-UM-EMAIL09.um.umsystem.edu} 10, 27 -- X-MS-Has-Attach: 10, 27 -- X-MS-TNEF-Correlator: 10, 27 -- Thread-Topic: Cryo SEM: Freeze drying question 10, 27 -- Thread-Index: AcWvyWPprjZOorSJSKWVU9++Jz1lzA== 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 27 -- To: {microscopy-at-microscopy.com} 10, 27 -- X-OriginalArrivalTime: 02 Sep 2005 14:19:56.0004 (UTC) FILETIME=[648AEA40:01C5AFC9] 10, 27 -- X-Proofpoint-Spam-Details: rule=tagforward_notspam policy=tagforward score=0 mlx=0 adultscore=0 adjust=0 reason=mlx engine=3.0.0-05090100 definitions=3.0.0-05090111 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j82EM6an026461 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 27 -- From TindallR-at-missouri.edu Fri Sep 2 10:17:45 2005 18, 27 -- Received: from um-proofpoint2.um.umsystem.edu (um-proofpoint2.umsystem.edu [207.160.151.136]) 18, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82FHjRT005334 18, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 10:17:45 -0500 18, 27 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 18, 27 -- by um-proofpoint2.um.umsystem.edu (8.13.3/8.13.3) with ESMTP id j82FGuC2003808 18, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 10:17:39 -0500 18, 27 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 18, 27 -- Fri, 2 Sep 2005 10:17:30 -0500 18, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 18, 27 -- Content-class: urn:content-classes:message 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain; 18, 27 -- charset="us-ascii" 18, 27 -- Subject: FW: [Microscopy] Cryo SEM: Freeze drying question 18, 27 -- Date: Fri, 2 Sep 2005 10:17:30 -0500 18, 27 -- Message-ID: {BA876152E8653240BE8572E897083EE79803C3-at-UM-EMAIL09.um.umsystem.edu} 18, 27 -- X-MS-Has-Attach: 18, 27 -- X-MS-TNEF-Correlator: 18, 27 -- Thread-Topic: [Microscopy] Cryo SEM: Freeze drying question 18, 27 -- Thread-Index: AcWvyhaDbdU0Aga2TZKDbbYkbMDzFgABxySg 18, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 18, 27 -- To: {microscopy-at-microscopy.com} 18, 27 -- X-OriginalArrivalTime: 02 Sep 2005 15:17:30.0581 (UTC) FILETIME=[6FA18850:01C5AFD1] 18, 27 -- X-Proofpoint-Spam-Details: rule=tagforward_notspam policy=tagforward score=0 mlx=0 adultscore=0 adjust=0 reason=mlx engine=3.0.0-05090100 definitions=3.0.0-05090111 18, 27 -- Content-Transfer-Encoding: 8bit 18, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j82FHjRT005334 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 23 -- From sszewczyk-at-arl.army.mil Fri Sep 2 12:55:33 2005 29, 23 -- Received: from ARLABBH01.ds.arl.army.mil (arlabbh01.ds.arl.army.mil [192.12.65.18]) 29, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82HtWop010624 29, 23 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 12:55:32 -0500 29, 23 -- Received: from ARLABML02.arl.army.mil ([128.63.57.81]) by ARLABBH01.ds.arl.army.mil with Microsoft SMTPSVC(6.0.3790.1830); 29, 23 -- Fri, 2 Sep 2005 13:55:29 -0400 29, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 29, 23 -- content-class: urn:content-classes:message 29, 23 -- MIME-Version: 1.0 29, 23 -- Content-Type: text/plain; 29, 23 -- charset="us-ascii" 29, 23 -- Subject: RE: [Microscopy] FW: Cryo SEM: Freeze drying question 29, 23 -- Date: Fri, 2 Sep 2005 13:54:48 -0400 29, 23 -- Message-ID: {D38F50BB246D1943888C1C1EDD848E590A07BF-at-ARLABML02.DS.ARL.ARMY.MIL} 29, 23 -- X-MS-Has-Attach: 29, 23 -- X-MS-TNEF-Correlator: 29, 23 -- Thread-Topic: [Microscopy] FW: Cryo SEM: Freeze drying question 29, 23 -- Thread-Index: AcWv0cSjOvZc6gTARs6pOHEAd7yKGAAFAXtg 29, 23 -- From: "Szewczyk, Steven \(Cont, ARL/WMRD\)" {sszewczyk-at-arl.army.mil} 29, 23 -- To: {microscopy-at-microscopy.com} 29, 23 -- X-OriginalArrivalTime: 02 Sep 2005 17:55:29.0759 (UTC) FILETIME=[81A94EF0:01C5AFE7] 29, 23 -- Content-Transfer-Encoding: 8bit 29, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j82HtWop010624 ==============================End of - Headers==============================
What type of problems are you experiencing with Image Pro? We've been using IPP for the past eight years without much incident. Is it technical problems with the hardware/software, or is it a matter of training? Perhaps you're not aware of the new forum they provide to discuss issues and applications of their software. The URL for the forum is:
http://www.mediacy.com/ipp/ippforum/
This is an excellent source of information about the software that you can utilize before just tossing the several thousand dollars spent on it! Feel free to ask me off line and I'll see if I can help you also.
Regards,
David Bell Scientist Electron Microscopy Lab Millipore Corporation 80 Ashby Road Bedford, MA 01730 (781) 533-2108
mmckilli-at-smurfit.com 09/02/2005 12:27 PM Please respond to mmckilli-at-smurfit.com
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mmckilli-at-smurfit.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 2, 2005 at 08:32:34 ---------------------------------------------------------------------------
Question: We recently purchased a Nikon digital camera for optical microscopes as well as Image Pro image analysis software.
We have abandoned use of Image Pro software because of contant problems. Does anyone have recommendations for comparable image analysis software packages?
Your question is very broad. Are you unhappy with the results you are getting? If so, exactly what is wrong? And which "marine species" are you working with? Have you checked the literature on the species of interest to see what others are using and what kind of results they are getting? I think you will have to provide more information if you want a specific recommnedation.
Geoff
Sue.Tyler-at-noaa.gov wrote:
} Could you advise me on the best primary fixative to use for marine } species that need both light microscopy and TEM? Currently we are } fixing our specimens in 2.5%Glut/Form in cacodylate buffer and } bisecting the animal -half for em and half for light microscopy. We } perform histology on the LM half first, then if we need to retrieve the } em fixed tissue we have the ability. In an effort to reduce work load } and tissue storage do you have other suggestions? } } Sue Tyler } Dept. of Commerce } Cooperative Oxford Laboratory } Oxford, MD. } sue.tyler-at-noaa.gov } } } }
-- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 31 -- From mcauliff-at-umdnj.edu Fri Sep 2 14:03:06 2005 8, 31 -- Received: from mail01.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 8, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j82J36Y0027566 8, 31 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Sep 2005 14:03:06 -0500 8, 31 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 8, 31 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 53292230061 8, 31 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Sep 2005 15:03:05 -0400 (EDT) 8, 31 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 8, 31 -- by mail01.umdnj.edu (Proprietary) with ESMTP id 69BFDEC0B5 8, 31 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Sep 2005 15:03:03 -0400 (EDT) 8, 31 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 8, 31 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 8, 31 -- id {0IM700H01CYJFT-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 8, 31 -- for microscopy-at-msa.microscopy.com; Fri, 02 Sep 2005 15:03:03 -0400 (EDT) 8, 31 -- Received: from [127.0.0.1] ([10.138.2.240]) 8, 31 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 8, 31 -- 2004)) with ESMTP id {0IM700ITME92CK-at-Polaris.umdnj.edu} ; Fri, 8, 31 -- 02 Sep 2005 15:03:02 -0400 (EDT) 8, 31 -- Date: Fri, 02 Sep 2005 15:03:28 -0400 8, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 31 -- Subject: Re: [Microscopy] TEM best fixative question 8, 31 -- In-reply-to: {200509021746.j82HkkJ7005156-at-ns.microscopy.com} 8, 31 -- To: Sue.Tyler-at-noaa.gov, MicroscopyListserver {microscopy-at-msa.microscopy.com} 8, 31 -- Message-id: {4318A200.8080502-at-umdnj.edu} 8, 31 -- MIME-version: 1.0 8, 31 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 8, 31 -- Content-transfer-encoding: 7BIT 8, 31 -- X-Accept-Language: en-us, en 8, 31 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 8, 31 -- Gecko/20040804 Netscape/7.2 (ax) 8, 31 -- References: {200509021746.j82HkkJ7005156-at-ns.microscopy.com} ==============================End of - Headers==============================
We are considering buying a digital camera for imaging a wide range of subjects (isn't everyone!) though our first project is centered on chlamydomonas fluorescence work particularly chloroplast auto-fluorescence.
So far the optronics microfire looks like a good camera that might suit our needs and I would very much appreciate any comments on this camera from anyone who has had experience with one.
Many thanks in advance
Mark Brickley
==============================Original Headers============================== 6, 16 -- From mark-at-brickley.plus.com Sat Sep 3 15:19:49 2005 6, 16 -- Received: from ptb-relay04.plus.net (ptb-relay02.plus.net [212.159.14.213]) 6, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j83KJnxP020453 6, 16 -- for {Microscopy-at-microscopy.com} ; Sat, 3 Sep 2005 15:19:49 -0500 6, 16 -- Received: from [84.92.45.162] (helo=[10.0.1.3]) 6, 16 -- by ptb-relay04.plus.net with esmtp (Exim) id 1EBeUK-0006j2-Hp 6, 16 -- for Microscopy-at-microscopy.com; Sat, 03 Sep 2005 21:19:44 +0100 6, 16 -- Mime-Version: 1.0 (Apple Message framework v622) 6, 16 -- Content-Transfer-Encoding: 7bit 6, 16 -- Message-Id: {039f830d64d85790c6e7a5d6fd4691ad-at-brickley.plus.com} 6, 16 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 16 -- To: Microscopy-at-microscopy.com 6, 16 -- From: Mark Brickley {mark-at-brickley.plus.com} 6, 16 -- Subject: optronics microfire 6, 16 -- Date: Sat, 3 Sep 2005 21:19:42 +0100 6, 16 -- X-Mailer: Apple Mail (2.622) ==============================End of - Headers==============================
With LM, I would like to look at starch granules from the topside and bottomside of a slide, in order to get more information about shape.
I can do this by flipping the slide over, and raising it slightly off the platten so that the coverslip is not scraped off - but to relocate the same object on the slide might require a lot of searching.
Can anyone suggest a simple mathematical formula that can be used to calculate new co-ordinates from (a) the linear dimensions of the slide (L x W), and (b) the mm grid co-ordinates of the object on the slide?
Does a formula/conversion-table already exist for this?
Yours hopefully, Peter -- Peter Matthews (Dr) National Museum of Ethnology Senri Expo Park, Suita City Osaka 565-8511, Japan Tel. +81 6 6876-2151 (museum) Tel. +81 6 6876-8357 (Peter's office) Fax +81 6 6878-7503 (museum)
Websites:
The Research Cooperative http://www.researchco-op.co.nz
A meeting place for research writers, editors, translators and proofreaders
2003 Conference on Research Writing in Japan:
http://www.researchco-op.net/conference.html
==============================Original Headers============================== 13, 14 -- From pjm-at-gol.com Mon Sep 5 04:02:08 2005 13, 14 -- Received: from ns1.minpaku.ac.jp (ns1.minpaku.ac.jp [192.51.169.132]) 13, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j85926ZW006160 13, 14 -- for {microscopy-at-microscopy.com} ; Mon, 5 Sep 2005 04:02:07 -0500 13, 14 -- Received: from [172.17.10.39] by ns1.minpaku.ac.jp (8.11.7/3.7Wpl2-02042214) 13, 14 -- id j8591tH05821; Mon, 5 Sep 2005 18:01:55 +0900 (JST) 13, 14 -- Mime-Version: 1.0 13, 14 -- X-Sender: pjm-at-popmail.gol.com 13, 14 -- Message-Id: {a06110402bf41b832c52e-at-[172.17.10.39]} 13, 14 -- Date: Mon, 5 Sep 2005 18:02:34 +0900 13, 14 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 13, 14 -- From: Peter Matthews {pjm-at-gol.com} 13, 14 -- Subject: relocating objects on a flipped slide 13, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
In principle it is real simple x(new) = Length of slide - x(original) y should remain the same if the slide is perfectly rectangular and the geometry of the stage is perfect. You will need to correct for any discrepancy between zero on the stage scale and the end of the slide (x=0).
Chris
----- Original Message ----- X-from: {pjm-at-gol.com} To: {cjeffree-at-staffmail.ed.ac.uk} Sent: Monday, September 05, 2005 10:02 AM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bjg-at-cmm.uwa.edu.au) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 5, 2005 at 04:08:22 ---------------------------------------------------------------------------
The following two positions are available in my lab. Both are ongoing, ie permanent, in a fantastic place, and a dollar here is about a dollar anywhere. Happy to answer any queries.
Cheers
Brendan
SENIOR TECHNICIAN (REF: 980) CENTRE FOR MICROSCOPY AND MICROANALYSIS
o Ongoing appointment o Salary range: HEE Level 5 $44,204 - $49,149 p.a. o Closing date: Tuesday, 20 September 2005
The Centre for Microscopy and Microanalysis (CMM) supports application of light, electron and laser microscopy techniques to a wide range of research, see http: //cmm.uwa.edu.au, as part of major regional and national collaborations.
We are seeking a person who is: o Client and quality focused o Keen to embrace new responsibilities and develop new skills
Application Details: Interested applicants must obtain the application package and address the prerequisites and selection criteria. These essential details can be accessed from the vacancy page on http: //jobs.uwa.edu.au/ or the 24 hour ìhotlineî on 6488 3733. To discuss or clarify any aspects of the position please contact the Director of the CMM, Associate Professor Brendan Griffin on 6488 2770 or email admin-at-cmm.uwa.edu.au.
National Employer of Choice for Women
**********
ASSOCIATE LECTURER/LECTURER (REF: 983) CENTRE FOR MICROSCOPY AND MICROANALYSIS
o Tenurable appointment o Salary Range: Associate Lecturer Level A $42,843 - $58,140 p.a. (Minimum starting salary for appointee with PhD will be $54,163 p.a.) o Salary range: Lecturer Level B $61,201 - $72,678 p.a. o Closing date: Friday, 7 October 2005
A CAMECA nanoSIMS 50 high-resolution ion microprobe was installed in the Centre for Microscopy and Microanalysis (CMM) at The University of Western Australia, as part of the NANO-MNRF, in June, 2003. The NANO-MNRF (www.nano.org.au) is a Major National Research Facility that links advanced nano-scale characterisation equipment at the Universities of Sydney, New South Wales, Queensland, Western Australia (UWA) and Melbourne. The range of CMM microscopy instrumentation and expertise is extensive (see http: //cmm.uwa.edu.au) such that the centre and MNRF represent a premium environment for research services, research training and research programs. UWA is also a major partner in local research consortia in isotope science with a range of stable and radiogenic isotope facilities, including two SHRIMP-II. We are seeking a highly motivated, self-guided scientist who has the ability to work with other staff within the Centre and its users.
The prime responsibility will be to manage a CAMECA nanoSIMS 50 ion microprobe as a world-class National Facility. The position will have the core role in a strong team led locally by Associate Professor Brendan Griffin and nationally by Professor Simon Ringer (NANO MNRF Executive Director). The level of appointment will be based on qualifications and experience. For appointment at Level A applicants must have a relevant degree preferably with a PhD and for appointment at Level B applicants must have a relevant degree with a PhD. Applicants with teaching experience are requested to submit a teaching portfolio as part of their application. For further information regarding the position please contact the Director of the CMM, Associate Professor Brendan Griffin on (08) 6488 2770 or email bjg-at-cmm.uwa.edu.au.
Located adjacent to the picturesque banks of the Swan River, the University offers an attractive benefits package including generous superannuation and leave provisions, fares to Perth (if applicable) for appointee and dependants along with a removals allowance and an enviable working environment. These and other benefits will be specified in the offer of employment.
APPLICATION DETAILS: For copies of the position description please access the website http: //jobs.uwa.edu.au/. Applicants must address the prerequisites and selection criteria. Written applications quoting the reference number, personal contact details, qualifications and experience, along with contact details of three referees should be sent to Director, Human Resources, The University of Western Australia, M350, 35 Stirling Highway, Crawley WA 6009 or emailed to jobs-at-uwa.edu.au by the closing date.
In our group (polymer physics) a long time ago, freeze drying of para-xylene (m.p. 19°C, b.p. ~ 144°C) was used. There are quite a few organic solvents freezing around 0°C, and many of them should respond to this technique. What is important is the vapour pressure, so things with too high a b.p. wouldn't work.
However, as the other replies state, it's the pumping system one should worry about.
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
==============================Original Headers============================== 6, 21 -- From hinmeigeng-at-hotmail.com Tue Sep 6 02:47:20 2005 6, 21 -- Received: from hotmail.com (bay101-f29.bay101.hotmail.com [64.4.56.39]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j867lJdq019622 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Sep 2005 02:47:20 -0500 6, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 6, 21 -- Tue, 6 Sep 2005 00:47:19 -0700 6, 21 -- Message-ID: {BAY101-F29A5D24BDCF13A6EAD321ECAA70-at-phx.gbl} 6, 21 -- Received: from 64.4.56.200 by by101fd.bay101.hotmail.msn.com with HTTP; 6, 21 -- Tue, 06 Sep 2005 07:47:18 GMT 6, 21 -- X-Originating-IP: [64.4.56.200] 6, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 6, 21 -- X-Sender: hinmeigeng-at-hotmail.com 6, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk 6, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 6, 21 -- To: Microscopy-at-MSA.Microscopy.Com 6, 21 -- Cc: TindallR-at-missouri.edu, R.H.Olley-at-reading.ac.uk 6, 21 -- Subject: Re: Cryo SEM: Freeze drying 6, 21 -- Date: Tue, 06 Sep 2005 07:47:18 +0000 6, 21 -- Mime-Version: 1.0 6, 21 -- Content-Type: text/plain; format=flowed 6, 21 -- X-OriginalArrivalTime: 06 Sep 2005 07:47:19.0015 (UTC) FILETIME=[3522A770:01C5B2B7] ==============================End of - Headers==============================
Does anyone else miss seeing the answers to questions. Now that replies do not go to the list we are missing out on a lot. Folks need to remember to add the list to their replies, unless the reply is intended only for the original sender
-- Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 Phone: 352-392-1295 Fax: 352 846 0251
==============================Original Headers============================== 4, 21 -- From gwe-at-ufl.edu Tue Sep 6 09:41:49 2005 4, 21 -- Received: from smtp.ufl.edu (sp44en1.nerdc.ufl.edu [128.227.74.44]) 4, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j86Efmpk032547 4, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Sep 2005 09:41:48 -0500 4, 21 -- Received: from [127.0.0.1] (pc2524c.dhcp.clas.ufl.edu [128.227.60.197]) 4, 21 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j86EfjP0137506 4, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Sep 2005 10:41:47 -0400 4, 21 -- Message-ID: {431DAAB0.9070107-at-ufl.edu} 4, 21 -- Date: Tue, 06 Sep 2005 10:41:52 -0400 4, 21 -- From: Greg Erdos {gwe-at-ufl.edu} 4, 21 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 4, 21 -- X-Accept-Language: en-us, en 4, 21 -- MIME-Version: 1.0 4, 21 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 4, 21 -- Subject: Replies 4, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- X-Spam-Status: hits=-0.904, required=5, tests=BAYES_30 4, 21 -- X-UFL-Spam-Status: hits=-0.904, required=5, tests=BAYES_30 4, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 4, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
IPP is a powerful application and of course depending on the version you are using, it may have some limitations (by features that you may not able to program for yourself). However IPP is as powerful as the user can use it. There are other applications out there and one application that is not driven by programming ability is MetaMorph by a company that used to be called Universal Imaging, recently it has been under a company called Molecular Devices. Check it out but don't give up on IPP so easily.
Ciao! Nico.
-----Original Message----- X-from: mmckilli-at-smurfit.com [mailto:mmckilli-at-smurfit.com] Sent: Friday, September 02, 2005 12:29 PM To: Ranieri, Nicola
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mmckilli-at-smurfit.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 2, 2005 at 08:32:34 ---------------------------------------------------------------------------
Question: We recently purchased a Nikon digital camera for optical microscopes as well as Image Pro image analysis software.
We have abandoned use of Image Pro software because of contant problems. Does anyone have recommendations for comparable image analysis software packages?
Thanks to everyone who replied to my question about purchasing flatbed scanner to scan TEM negatives. Most of you suggested EPSON and Canon scanners. So we will go with one with a reasonable price tag.
Best wishes,
Soumitra
************************************************************* Soumitra Ghoshroy College Associate Professor, Biology Director, Electron Microscopy Lab Box 3EML New Mexico State University Las Cruces, NM 88003 Tel: 505-646-3268 (office), 646-3283 (lab) Fax: 505-646-3282 e-mail:sghoshro-at-nmsu.edu http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm http://emldata.nmsu.edu/eml/
==============================Original Headers============================== 4, 20 -- From sghoshro-at-NMSU.Edu Tue Sep 6 10:29:58 2005 4, 20 -- Received: from ccserver2.nmsu.edu (ccserver2.nmsu.edu [128.123.34.53]) 4, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j86FTwJn015258 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Sep 2005 10:29:58 -0500 4, 20 -- Received: from ccserver2.nmsu.edu (localhost.localdomain [127.0.0.1]) 4, 20 -- by localhost (Postfix) with SMTP id 5875735EAF1 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Sep 2005 09:29:58 -0600 (MDT) 4, 20 -- Received: from verdi (verdi.nmsu.edu [128.123.34.188]) 4, 20 -- by ccserver2.nmsu.edu (Postfix) with ESMTP id 3181435EAC3 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Sep 2005 09:29:56 -0600 (MDT) 4, 20 -- Date: Tue, 6 Sep 2005 09:29:56 -0600 (MDT) 4, 20 -- From: sghoshro-at-NMSU.Edu 4, 20 -- X-X-Sender: sghoshro-at-verdi 4, 20 -- To: MSA Listserve {Microscopy-at-MSA.Microscopy.Com} 4, 20 -- Subject: Thank you for scanner info 4, 20 -- Message-ID: {Pine.GSO.4.61.0509060923560.9647-at-verdi} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 4, 20 -- X-PMX-Version: 5.0.3.165339, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.9.6.15 4, 20 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='NO_REAL_NAME 0, __C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0' ==============================End of - Headers==============================
I would consider the Zeiss AxioVision 4.0 software. Accurate, very user-friendly and with quite a lot of basic functions that can be even more expanded. Wizards and record-functions help you to automate repetitive analysis... I have no personal benefit if you would start using this software, don't get me wrong, it just 'dazzled' me with a few things in such a way that I even bought the VBA-module to start programming in Visual Basic to even more expand it's possibilities! Besides this, a very helpful forum has been created to exchange questions, answers, programs etc., just as this forum. Best,
Sven Terclavers
Quoting NRANIERI-at-ORA.FDA.GOV:
} } } } ---------------------------------------------------------------------- ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- ------ } } IPP is a powerful application and of course depending on the version } you are } using, it may have some limitations (by features that you may not } able to } program for yourself). However IPP is as powerful as the user can use } it. } There are other applications out there and one application that is } not } driven by programming ability is MetaMorph by a company that used to } be } called Universal Imaging, recently it has been under a company } called } Molecular Devices. Check it out but don't give up on IPP so easily. } } Ciao! } Nico. } } -----Original Message----- } X-from: mmckilli-at-smurfit.com [mailto:mmckilli-at-smurfit.com] } Sent: Friday, September 02, 2005 12:29 PM } To: Ranieri, Nicola } Subject: [Microscopy] viaWWW: Image analysis software } } } } } ---------------------------------------------------------------------- ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- ------ } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (mmckilli-at-smurfit.com) from } http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, } } September 2, 2005 at 08:32:34 } ---------------------------------------------------------------------- ----- } } Email: mmckilli-at-smurfit.com } Name: M McKillip } } Title-Subject: [Filtered] Image analysis software... } } Question: We recently purchased a Nikon digital camera for optical } microscopes as well as Image Pro image analysis software. } } We have abandoned use of Image Pro software because of contant } problems. Does anyone have recommendations for comparable image } analysis software packages? } } ---------------------------------------------------------------------- ----- } } ==============================Original } Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Fri Sep 2 11:26:50 2005 } 6, 12 -- Received: from [192.168.2.125] (msdvpn22.msd.anl.gov } [130.202.238.86]) } 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j82GQlLr018919 } 6, 12 -- for {microscopy-at-microscopy.com} ; Fri, 2 Sep 2005 11:26:48 } -0500 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06020405bf3e2db6235c-at-[192.168.2.125]} } 6, 12 -- Date: Fri, 2 Sep 2005 11:26:45 -0500 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: mmckilli-at-smurfit.com (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: Image analysis software } 6, 12 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers============================== } } ==============================Original } Headers============================== } 13, 16 -- From NRANIERI-at-ORA.FDA.GOV Tue Sep 6 10:25:46 2005 } 13, 16 -- Received: from wall3-pub.fda.gov (wall3-pub.fda.gov } [150.148.0.65]) } 13, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j86FPjuL008593 } 13, 16 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Sep 2005 10:25:46 } -0500 } 13, 16 -- Received: from orshq08a.fda.gov by wall3-pub.fda.gov } 13, 16 -- via smtpd (for microscopy.com [206.69.208.10]) } with ESMTP; Tue, 6 Sep 2005 11:25:45 -0400 } 13, 16 -- Received: by orshq08a.fda.gov with Internet Mail Service } (5.5.2657.72) } 13, 16 -- id {Q5ZM6G27} ; Tue, 6 Sep 2005 11:25:43 -0400 } 13, 16 -- Message-ID: } {0418220A22838F46B3F8284F21EAF8D501603F79-at-orscrcindo02.fda.gov} } 13, 16 -- From: "Ranieri, Nicola" {NRANIERI-at-ORA.FDA.GOV} } 13, 16 -- To: "'Microscopy-at-microscopy.com'" } {Microscopy-at-microscopy.com} } 13, 16 -- Subject: RE: [Microscopy] viaWWW: Image analysis software } 13, 16 -- Date: Tue, 6 Sep 2005 11:25:19 -0400 } 13, 16 -- MIME-Version: 1.0 } 13, 16 -- X-Mailer: Internet Mail Service (5.5.2657.72) } 13, 16 -- Content-Type: text/plain } ==============================End of - } Headers============================== } }
Hi everyone, In my experience, DAPI very specifically stains DNA nicely. I have also used other DNA stains like Sytox but I get better definition in DNA/nuclear labeling with DAPI when taking epifluorescent micrographs.
For what concentration of DAPI to use in your specimen, I would recommend a PubMed search.
I hope this helps.
With much sincerity, Carlo
Carlo Franco Bolivar Balane
Box 4058, 222 Church Street, or Wolfe Laboratory Wesleyan University Station Rm. 157, HA Laboratories Middletown, CT, 06459-4058 Wesleyan University phone: 1.860.759.2830 phone: 1.860.685.3275
==============================Original Headers============================== 9, 35 -- From cbalane-at-wesleyan.edu Tue Sep 6 12:14:00 2005 9, 35 -- Received: from post2.wesleyan.edu (post2.wesleyan.edu [129.133.6.128]) 9, 35 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j86HDxH6001542 9, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Sep 2005 12:14:00 -0500 9, 35 -- Received: from localhost.localdomain (pony4.wesleyan.edu [129.133.6.195]) 9, 35 -- by post2.wesleyan.edu (8.12.11/8.12.11) with ESMTP id j86HDu3A016287; 9, 35 -- Tue, 6 Sep 2005 13:13:56 -0400 9, 35 -- Received: from localhost.localdomain (pony4 [127.0.0.1]) 9, 35 -- by localhost.localdomain (8.12.11/8.12.11) with ESMTP id j86HDvIJ007998; 9, 35 -- Tue, 6 Sep 2005 13:13:57 -0400 9, 35 -- Received: (from apache-at-localhost) 9, 35 -- by localhost.localdomain (8.12.11/8.12.11/Submit) id j86HDvGC007996; 9, 35 -- Tue, 6 Sep 2005 13:13:57 -0400 9, 35 -- Received: from 129.133.93.129 9, 35 -- (SquirrelMail authenticated user cbalane); 9, 35 -- by webmail.wesleyan.edu with HTTP; 9, 35 -- Tue, 6 Sep 2005 13:13:57 -0400 (EDT) 9, 35 -- Message-ID: {2466.129.133.93.129.1126026837.squirrel-at-129.133.93.129} 9, 35 -- In-Reply-To: {200509060011.j860BojL015020-at-ns.microscopy.com} 9, 35 -- References: {200509060011.j860BojL015020-at-ns.microscopy.com} 9, 35 -- Date: Tue, 6 Sep 2005 13:13:57 -0400 (EDT) 9, 35 -- Subject: Re: [Microscopy] LM- DAPI staining of cell inclusions? 9, 35 -- From: "Balane, Carlo Franco Bolivar" {cbalane-at-wesleyan.edu} 9, 35 -- To: sheris-at-MIT.EDU 9, 35 -- Cc: Microscopy-at-microscopy.com 9, 35 -- User-Agent: SquirrelMail/1.4.3a-0.e3.1 9, 35 -- X-Mailer: SquirrelMail/1.4.3a-0.e3.1 9, 35 -- MIME-Version: 1.0 9, 35 -- Content-Type: text/plain;charset=iso-8859-1 9, 35 -- Content-Transfer-Encoding: 8bit 9, 35 -- X-Priority: 3 (Normal) 9, 35 -- Importance: Normal 9, 35 -- X-Wesleyan-MailScanner-Information: Please contact the ISP for more information 9, 35 -- X-Wesleyan-MailScanner: Found to be clean 9, 35 -- X-MailScanner-From: cbalane-at-wesleyan.edu ==============================End of - Headers==============================
I concur with you Greg, I personally prefer the old method. However, the change was made about a month ago as we were getting far too many inadvertent postings and the rash that occurred at the end of July was getting just too much, particularly with the almost immediate followup of an apology.
For the time being, just a reminder to all subscribers. Please remember (and this is documented in the Instructions if you are replying to a posting and consider the information of general use to everyone, remember to change the reply to address from the senders address (automatically provided by your Email client) to include
microscopy-at-microscopy.com
It is also appropriate should an individual collects a series of answers /solutions to their question that (s)he compose a summary of the responses and post that back to the list. This way it will be included in the archives and provide a convenient mechanism for others to locate a synopsis of the question and answers using the search engine
Cheers
Nestor Your Friendly Neighborhood SysOp
At 9:41 AM -0500 9/6/05, gwe-at-ufl.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ishask2aol.com) from http://microscopy.com/MLFormMail.html on Tuesday, September 6, 2005 at 00:24:01 ---------------------------------------------------------------------------
Email: ishask2aol.com Name: isha
Organization: san joaquin delta college, electron microscopy program
Title-Subject: [Filtered] fixing and viewing plant on tem
Question: i am a EM student at san joaquin delta college in CA. we have a project coming up where we have to fix, section, stain and view plant tissue on the tem. so i was wondering which part of a plant will be interesting to look at...or is there a particular plant that i should look for thanks isha
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rberry-at-rsbs.anu.edu.au) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, September 6, 2005 at 20:57:06 ---------------------------------------------------------------------------
Email: rberry-at-rsbs.anu.edu.au Name: Richard Berry
Organization: CVS, RSBS, ANU
Title-Subject: [Filtered] en-bloc stains for light microscopy
Question: Hello there,
I was just wondering if anyone had ever happened upon a reliable method for en-bloc staining insect neural tissue in araldite embedded specimens.
I have tried a few things so far, including, toluidine blue, ferricyanide and p-phenylaminediamine. While these seem to help none of them give satisfactory contrast without post-staining the sections with tol. blue.
Does anyone know of something that will stain membrane structure well and can be used en-bloc? The specimens do not have to be embedded in araldite embedded...just something that will cut 1-2 um sections.
Dear Isha, Well, as a plant biologist, I have to say that *all* parts of the plant are interesting! But perhaps it is worth mentioning that root tips may be easier to fix and prep than other parts because they lack cuticles, air spaces, and wood, all of which can give rise to sample prep issues. Of course, all of these other parts can be preped too so follow your own curiosity.
Tobias
} } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (ishask2aol.com) from } http://microscopy.com/MLFormMail.html on Tuesday, September 6, 2005 } at 00:24:01 } --------------------------------------------------------------------------- } } Email: ishask2aol.com } Name: isha } } Organization: san joaquin delta college, electron microscopy program } } Title-Subject: [Filtered] fixing and viewing plant on tem } } Question: i am a EM student at san joaquin delta college in CA. we } have a project coming up where we have to fix, section, stain and } view plant tissue on the tem. } so i was wondering which part of a plant will be interesting to look } at...or is there a particular plant that i should look for } thanks } isha } } } --------------------------------------------------------------------------- }
The usual en bloc stains used for TEM work probably won't give you enough contrast for LM unless you try phase contrast or Nomarski illumination. p-phenylenediamine will reduce the osmium in the tissue and increase contrast but is not a stain in the usual sense. If you used ferricyanide-osmium and en bloc staining with uranyl acetate you might get enough contrast for your needs. Only you can make that judgement. Toluidine blue is leached out of tissue very rapidly in the ascending ethanols used for dehydration. Try Cresyl Violet or Thionin instead OR dehydrate in acetone instead of alcohols. I don't think either will show up (much) in the EM though.
Geoff
rberry-at-rsbs.anu.edu.au wrote:
} Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (rberry-at-rsbs.anu.edu.au) from } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on } Tuesday, September 6, 2005 at 20:57:06 } --------------------------------------------------------------------------- } } Email: rberry-at-rsbs.anu.edu.au } Name: Richard Berry } } Organization: CVS, RSBS, ANU } } Title-Subject: [Filtered] en-bloc stains for light microscopy } } Question: Hello there, } } I was just wondering if anyone had ever happened upon a reliable } method for en-bloc staining insect neural tissue in araldite embedded } specimens. } } I have tried a few things so far, including, toluidine blue, } ferricyanide and p-phenylaminediamine. While these seem to help none } of them give satisfactory contrast without post-staining the sections } with tol. blue. } } Does anyone know of something that will stain membrane structure well } and can be used en-bloc? The specimens do not have to be embedded in } araldite embedded...just something that will cut 1-2 um sections. } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 12 -- From zaluzec-at-microscopy.com Wed Sep 7 03:28:23 2005 } 9, 12 -- Received: from [131.111.102.55] (msdvpn24.msd.anl.gov [130.202.238.88]) } 9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j878SLDS030506 } 9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 7 Sep 2005 03:28:22 -0500 } 9, 12 -- Mime-Version: 1.0 } 9, 12 -- X-Sender: (Unverified) } 9, 12 -- Message-Id: {p06020404bf445515eb94-at-[131.111.102.55]} } 9, 12 -- Date: Wed, 7 Sep 2005 03:28:20 -0500 } 9, 12 -- To: microscopy-at-microscopy.com } 9, 12 -- From: rberry-at-rsbs.anu.edu.au (by way of MicroscopyListserver) } 9, 12 -- Subject: viaWWW: en-bloc stains for light microscopy } 9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 10, 32 -- From mcauliff-at-umdnj.edu Wed Sep 7 09:20:39 2005 10, 32 -- Received: from mail01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 10, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j87EKdGx026319 10, 32 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Sep 2005 09:20:39 -0500 10, 32 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 10, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id EEE02EC06E 10, 32 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Sep 2005 10:20:38 -0400 (EDT) 10, 32 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 10, 32 -- by mail01.umdnj.edu (Proprietary) with ESMTP id D46B0EC055 10, 32 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Sep 2005 10:20:32 -0400 (EDT) 10, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 10, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 10, 32 -- id {0IMG00D01ADI48-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 10, 32 -- for microscopy-at-msa.microscopy.com; Wed, 07 Sep 2005 10:20:32 -0400 (EDT) 10, 32 -- Received: from [127.0.0.1] ([10.138.2.240]) 10, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 10, 32 -- 2004)) with ESMTP id {0IMG00AQYAC06J-at-Polaris.umdnj.edu} ; Wed, 10, 32 -- 07 Sep 2005 10:16:49 -0400 (EDT) 10, 32 -- Date: Wed, 07 Sep 2005 10:17:12 -0400 10, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 10, 32 -- Subject: Re: [Microscopy] viaWWW: en-bloc stains for light microscopy 10, 32 -- In-reply-to: {200509070829.j878TSIs002286-at-ns.microscopy.com} 10, 32 -- To: rberry-at-rsbs.anu.edu.au, 10, 32 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 10, 32 -- Message-id: {431EF668.70607-at-umdnj.edu} 10, 32 -- MIME-version: 1.0 10, 32 -- Content-type: text/plain; format=flowed; charset=us-ascii 10, 32 -- Content-transfer-encoding: 7BIT 10, 32 -- X-Accept-Language: en-us, en 10, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 10, 32 -- Gecko/20040804 Netscape/7.2 (ax) 10, 32 -- References: {200509070829.j878TSIs002286-at-ns.microscopy.com} ==============================End of - Headers==============================
I understand that a true plant biologist such as Tobias would find all plant tissues of interest. However, I started out as an animal person so love lots of "stuff" in my images.
Many plant cells have huge internal vacuoles and relatively few organelles so can be very uninteresting to many people. However, if you start out with very young tissue, such as the very young leaves from a bean plant, than you will find lots of interesting stuff.
In this case, the leaves will be so thin that you will be able to prepare and microtome x-sections through the entire leaf. You will see the different cell types and arrangements toward both the top and bottom leaf portions. You will also get lots of chloroplasts, nuclei, a fair number of mitochondria and other organelles. You should be able to find golgi, etc. If you are lucky you will cut through stomata as well.
This can be lots of fun as well as informative. Good luck!
I would recommend a half strength Karnovsky fixative in Cacodylate buffer followed by osmium and possibly en block uranyl acetate staining. You might need something to control the osmolarity such as NaCl. Check the literature for details. Embedding should be in Spurr resin or other low viscosity resin as plant walls can be difficult to infiltrate. Use a very gradual infiltration, increasing resin concentration very slowly with gentle rotation/agitation until you have reached 50% resin and then can go a bit faster.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
On 9/7/05 3:30 AM, "ishask2aol.com-at-ns.microscopy.com" {ishask2aol.com-at-ns.microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (ishask2aol.com) from } http://microscopy.com/MLFormMail.html on Tuesday, September 6, 2005 } at 00:24:01 } --------------------------------------------------------------------------- } } Email: ishask2aol.com } Name: isha } } Organization: san joaquin delta college, electron microscopy program } } Title-Subject: [Filtered] fixing and viewing plant on tem } } Question: i am a EM student at san joaquin delta college in CA. we } have a project coming up where we have to fix, section, stain and } view plant tissue on the tem. } so i was wondering which part of a plant will be interesting to look } at...or is there a particular plant that i should look for } thanks } isha } } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 12 -- From zaluzec-at-microscopy.com Wed Sep 7 03:27:56 2005 } 8, 12 -- Received: from [131.111.102.55] (msdvpn24.msd.anl.gov } [130.202.238.88]) } 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j878Rtke029585 } 8, 12 -- for {microscopy-at-microscopy.com} ; Wed, 7 Sep 2005 03:27:55 -0500 } 8, 12 -- Mime-Version: 1.0 } 8, 12 -- X-Sender: (Unverified) } 8, 12 -- Message-Id: {p06020403bf4454fae512-at-[131.111.102.55]} } 8, 12 -- Date: Wed, 7 Sep 2005 03:27:53 -0500 } 8, 12 -- To: microscopy-at-microscopy.com } 8, 12 -- From: ishask2aol.com-at-ns.microscopy.com (by way of } MicroscopyListserver) } 8, 12 -- Subject: viaWWW: fixing and viewing plant on tem } 8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
Nestor, Is there any way to make 'Reply All' include the listserver address along with the correspondent's address?
It will be interesting to see if mis-addressed replies increase on the other microscopy-related listservers that retain the old reply mechanism.
Regards, Glen Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires Windows 95 or better", so I bought a Macintosh. ************************************************************************ ******
On Sep 7, 2005, at 1:23 AM, zaluzec-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Greg etal.... } } I concur with you Greg, I personally prefer the old method. } However, the change was made about a month ago } as we were getting far too many inadvertent postings } and the rash that occurred at the end of July was getting just } too much, particularly with the almost immediate } followup of an apology. } } For the time being, just a reminder to all subscribers. } Please remember (and this is documented in the Instructions } if you are replying to a posting and consider the information of } general use to everyone, remember to change the reply to address } from the senders address (automatically provided by your Email } client) to include } } microscopy-at-microscopy.com } } } It is also appropriate should an individual collects a series of } answers } /solutions to their question that (s)he compose a summary of } the responses and post that back to the list. This way it will be } included in the } archives and provide a convenient mechanism for others to } locate a synopsis of the question and answers using the search } engine } } Cheers } } Nestor } Your Friendly Neighborhood SysOp } } } } } } At 9:41 AM -0500 9/6/05, gwe-at-ufl.edu wrote: } } } --------------------------------------------------------------------- } } ------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } ------- } } } } Does anyone else miss seeing the answers to questions. Now that } } replies } } do not go to the list we are missing out on a lot. Folks need to } } remember to add the list to their replies, unless the reply is } } intended } } only for the original sender } } } } -- } } Gregory W. Erdos, Ph.D. } } Assistant Director, Biotechnology Program } } Scientific Director, Electron Microscopy } } P.O. Box 118525 } } 217 Carr Hall } } University of Florida } } Gainesville, FL 32611 } } Phone: 352-392-1295 } } Fax: 352 846 0251 } } } } } } } } } } ==============================Original } Headers============================== } 13, 13 -- From zaluzec-at-microscopy.com Wed Sep 7 03:20:53 2005 } 13, 13 -- Received: from [131.111.102.55] (msdvpn24.msd.anl.gov } [130.202.238.88]) } 13, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j878KqWV021326 } 13, 13 -- for {microscopy-at-microscopy.com} ; Wed, 7 Sep 2005 } 03:20:53 -0500 } 13, 13 -- Mime-Version: 1.0 } 13, 13 -- Message-Id: {p06020401bf445109f8d4-at-[131.111.102.55]} } 13, 13 -- In-Reply-To: {200509061441.j86Efnod032561-at-ns.microscopy.com} } 13, 13 -- References: {200509061441.j86Efnod032561-at-ns.microscopy.com} } 13, 13 -- Date: Wed, 7 Sep 2005 03:20:50 -0500 } 13, 13 -- To: microscopy-at-microscopy.com } 13, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} } 13, 13 -- Subject: Administrivia: Replies } 13, 13 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers============================== }
I need to clarify my fixation question. First, I am trying to fix marine amphipods for both light and TEM. Currently I am fixing them in 2.5% Gluteraldehyde and 2.5% Formaldehyde in sodium cacodylate buffer and bisecting the animal and placing half in 70% EtOH for light microscopy and the other half in cacodylic buffer. I then process the animals for light and if the pathologist sees anything interesting I will have the other half of the animal fixed for any em work. I am not doing any immuno.
The fixation is fine, but is there a less labor intensive way? I don't like storing my tissue in the buffer because it molds after about 8 months.
I appreciate all of your questions and I look forward to your comments.
Sue
==============================Original Headers============================== 4, 18 -- From sue.tyler-at-noaa.gov Wed Sep 7 13:16:56 2005 4, 18 -- Received: from hermes.nos.noaa.gov (hermes.nos.noaa.gov [140.90.119.204]) 4, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j87IGuRV022053 4, 18 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Sep 2005 13:16:56 -0500 4, 18 -- Received: from [10.60.12.125] ([10.60.12.125]) by 4, 18 -- hermes.nos.noaa.gov (Netscape Messaging Server 4.15) with ESMTP 4, 18 -- id IMGLG700.6FX for {microscopy-at-msa.microscopy.com} ; Wed, 7 Sep 4, 18 -- 2005 14:16:55 -0400 4, 18 -- Message-ID: {431F2E97.8000101-at-noaa.gov} 4, 18 -- Date: Wed, 07 Sep 2005 14:16:55 -0400 4, 18 -- From: "Sue Tyler" {Sue.Tyler-at-noaa.gov} 4, 18 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 4, 18 -- X-Accept-Language: en-us, en 4, 18 -- MIME-Version: 1.0 4, 18 -- To: microscopy-at-msa.microscopy.com 4, 18 -- Subject: Clarification of Marine TEM fixative 4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
} Sue, } } I misplaced your reply to me, however, I must ask a couple more questions. } I assume you are processing the 70% half into paraffin and the other half } goes into resin. } } Rather than process all tissues in plastic, I assume the material needs to } be scanned by the pathologist with the light microscope. If he or she finds } areas of interest, you are then required to process the other half for } ultrastructural TEM examination: ok so far? } } Are you specifically embedding separately because of the size of the } amphipods or because you are required to stain with either H&E or other more } specific stains. } } If it is a size related issue, you will more than likely need to process } both halves to paraffin and resin. } } If it is a stain related issue, you may want to try embedding in LR White } methylmethacrylate resin. This resin is quite different from epoxy resins } in that you can use a acid fuchsin/methylene blue stain to produce an H&E } like stained section. } } If you pathologist is not too concerned with leaching of material over time, } you might be better off leaving your specimens in your primary fixative } instead of cacodylate buffer. If you are getting growth in your buffer over } time, you must be stored for several months. Even this length of time will } leach proteins. } } I asked about your use or if you use a digital ccd camera on your TEM } because the ccd will provide incredible contrast. I used to use a } glutaraldehyde/osmium/tannic acid/osmium fixative for contrast and } penetration. With the digital camera, I don't need the extra contrast. } } Regards, } Ken } _______________________________________ } Kenneth L. Tiekotter, Adjunct Professor } The University of Portland } Department of Biology } 5000 N Willamette Blvd. } Portland, OR 97203 USA } } Tel.: 503.943.8861 } Email: tiekotte-at-up.edu } } } On 9/7/05 11:17 AM, "Sue.Tyler-at-noaa.gov" {Sue.Tyler-at-noaa.gov} wrote: } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 2, 19 -- From sue.tyler-at-noaa.gov Wed Sep 7 14:28:10 2005 2, 19 -- Received: from hermes.nos.noaa.gov (hermes.nos.noaa.gov [140.90.119.204]) 2, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j87JSARP031904 2, 19 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Sep 2005 14:28:10 -0500 2, 19 -- Received: from [10.60.12.125] ([10.60.12.125]) by 2, 19 -- hermes.nos.noaa.gov (Netscape Messaging Server 4.15) with ESMTP 2, 19 -- id IMGOQY00.RL5; Wed, 7 Sep 2005 15:28:10 -0400 2, 19 -- Message-ID: {431F3F49.2020200-at-noaa.gov} 2, 19 -- Date: Wed, 07 Sep 2005 15:28:09 -0400 2, 19 -- From: "Sue Tyler" {Sue.Tyler-at-noaa.gov} 2, 19 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 2, 19 -- X-Accept-Language: en-us, en 2, 19 -- MIME-Version: 1.0 2, 19 -- To: tiekotte-at-up.edu, microscopy-at-msa.microscopy.com 2, 19 -- Subject: Re: [Microscopy] Clarification of Marine TEM fixative 2, 19 -- References: {BF448709.2977%tiekotte-at-up.edu} 2, 19 -- In-Reply-To: {BF448709.2977%tiekotte-at-up.edu} 2, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am writing to invite you to attend a day long symposium on advanced biomedical imaging techniques. The venue for this is the annual Research and Industrial Collaboration Conference (RICC) sponsored by CenSSIS and held at Northeastern University in Boston. The RICC is a two day affair (October 6‑7). The biomedical imaging symposium will take place on Friday, October 7th (Day Two of the RICC). The morning session of the symposium will discuss stem cell imaging and will include speakers such as Gerald Schatten (a member of the team that reported the recent canine cloning in South Korea). The afternoon will focus upon techniques for multi spectral cellular imaging and will include speakers from both academia and industry.
A complete agenda listing, directions and registration form can be found on the web link: {http://www.censsis.neu.edu/RICC/2005} http://www.censsis.neu.edu/RICC/2005.
A more general description of the CenSSIS research and education programs can be found on our homepage: {http://www.censsis.neu.edu/} http://www.censsis.neu.edu.
In addition to the presentations at the symposium, there will be an opportunity to learn about the 3D Keck Fusion Microscope (3DFM) facility which is located at Northeastern. The Keck 3DFM is a multi-modal microscope capable of imaging live samples in up to six different modes for at least 72 hours. Along with standard DIC and epifluorescence modes, we are imaging with single and two photon fluorescent excitation wavelengths from 454nm up to 1100nm. We also have a newly patented, noninvasive, and nontoxic imaging mode called Quadrature Microscopy. This specialized technique measures the phase change of light as it passes through a live specimen and yields comprehensive real time data. A link to the full description of the facility can be found on the CenSSIS homepage.
I hope that you will be able to attend our symposium. Don't hesitate to contact me if you need further information. The registration deadline is Sunday, October 1st.
**Important Note: All unused rooms will be released from the hotel room blocks on September 14, 2005. Please be sure to make your reservation as soon as possible. Room price and availability cannot be guaranteed beyond these dates.
Best,
Gary
Gary Laevsky, Ph.D. Keck Facility Manager, CenSSIS Northeastern University 302 Stearns 360 Huntington Ave. Boston, MA 02115 voice(617) 373 - 2589 {br} fax(617) 373 - 7783 {br} {br}
http://www.censsis.neu.edu
http://www.ece.neu.edu/groups/osl
http://www.keck3dfm.neu.edu
==============================Original Headers============================== 34, 20 -- From glaevsky-at-gateway.ece.neu.edu Thu Sep 8 08:42:44 2005 34, 20 -- Received: from SMTP1.ECE.NEU.EDU (markov.ece.neu.edu [129.10.60.83]) 34, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j88Dghta020134 34, 20 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 8 Sep 2005 08:42:44 -0500 34, 20 -- Received: from calvin.ece.neu.edu (calvin.ece.neu.edu [129.10.62.61]) 34, 20 -- by SMTP1.ECE.NEU.EDU (8.12.5/8.12.5) with SMTP id j88DghEE007278 34, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 Sep 2005 09:42:43 -0400 (EDT) 34, 20 -- Received: from Ishkabbible.ece.neu.edu ([129.10.56.134]) 34, 20 -- by calvin.ece.neu.edu (SAVSMTP 3.1.0.29) with SMTP id M2005090809424213736 34, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 08 Sep 2005 09:42:42 -0400 34, 20 -- Message-Id: {6.2.1.2.2.20050908094138.021ab8d0-at-pop.ece.neu.edu} 34, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 34, 20 -- Date: Thu, 08 Sep 2005 09:42:42 -0400 34, 20 -- To: Microscopy-at-msa.microscopy.com 34, 20 -- From: glaevsky-at-ECE.NEU.EDU 34, 20 -- Subject: Research and Industrial Collaboration Conference (RICC) 34, 20 -- Mime-Version: 1.0 34, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 34, 20 -- Content-Transfer-Encoding: 8bit 34, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j88Dghta020134 ==============================End of - Headers==============================
Dear MSA members and other listers, As wrangler of the MSA video collection I am often asked if we have a full basic course in electron microscopy available on DVD. We do not. I can also infer from folks who purchase 20 or 30 or 40 or 50 DVDs that they intend to teach themselves what they need to know from our recorded tutorials. (The record purchase is 52 DVDs that was filled last month.). In light of the fact that EM coures are disappearing at universities around the country, the Education Committee of MSA is considering the idea of putting together a basic course or courses on electron microscopy and make them available on DVD. Something that would present the technology in an organized fashion and be comprehensive enough to put a student in a position to begin work in the area. If such a project is undertaken, Steve Barlow and Howard Berg have agreed to work with me on coordinating it. First, we would like to hear from the community on whether or not they think this is a good idea. Second we would be looking for volunteers who might be willing to record a lesson with supporting demonstrations and other visual representations that could flesh out the coverage. We don't want just a talking head. We would also be looking for folks who would be willing to share the syllabi from their courses so that we might determine how to go about organizing such a thing. Any and all feedback is welcome.
Regarsd to all, Greg
-- Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 Phone: 352-392-1295 Fax: 352 846 0251
==============================Original Headers============================== 7, 21 -- From gwe-at-ufl.edu Thu Sep 8 14:02:51 2005 7, 21 -- Received: from smtp.ufl.edu (sp42en1.nerdc.ufl.edu [128.227.74.42]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j88J2o5c032635 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 14:02:51 -0500 7, 21 -- Received: from [127.0.0.1] (pc2524c.dhcp.clas.ufl.edu [128.227.60.197]) 7, 21 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j88J2lEF127796 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 15:02:49 -0400 7, 21 -- Message-ID: {43208ADE.6050104-at-ufl.edu} 7, 21 -- Date: Thu, 08 Sep 2005 15:02:54 -0400 7, 21 -- From: Greg Erdos {gwe-at-ufl.edu} 7, 21 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 7, 21 -- X-Accept-Language: en-us, en 7, 21 -- MIME-Version: 1.0 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- Subject: Tutorials 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 7, 21 -- X-UFL-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 7, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 7, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Greg- Yea! I was on of those who purchased your DVD's and yes, I would love to have a DVD with the basic course. It would be great if you could improve the DVD quality, the info was great, the visual could be better. Good Idea! I hope you get a lot of response from the EM community.
Sue New User!
gwe-at-ufl.edu wrote:
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Hi, I'm researching SEM instruments and am wondering about yearly service contracts and how they change over the life of the instrument. Thoughts? Thanks, Ann.
Ann St. Amand, Ph.D., CLP President PhycoTech, Inc. 620 Broad St., Suite 100 St. Joseph, Michigan 49085 USA
You may want to try Geller MicroAnalytical: http://www.gellermicro.com/
Best regards-
David
-- David Henriks
South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA
TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499
email: henriks-at-southbaytech.com
Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.
Please visit us online at www.southbaytech.com.
The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.
SHem-at-laurentian.ca wrote:
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I think that that would be a great idea. However, I have another suggestion to add to it. I would like to see the original lesson broadcast over the internet where MSA members could "tune-in" to watch. Perhaps there could be a question and answer period from audience memebers that wold be included int he video also. Afterall, many of the original tapes were recorded at the meetings.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-------- Original Message -------- } From: gwe-at-ufl.edu } Sent: Thursday, September 08, 2005 3:09 PM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] Tutorials } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear MSA members and other listers, } As wrangler of the MSA video collection I am often asked if we have } a full basic course in electron microscopy available on DVD. We do } not. I can also infer from folks who purchase 20 or 30 or 40 or 50 } DVDs that they intend to teach themselves what they need to know from } our recorded tutorials. (The record purchase is 52 DVDs that was filled } last month.). In light of the fact that EM coures are disappearing at } universities around the country, the Education Committee of MSA is } considering the idea of putting together a basic course or courses on } electron microscopy and make them available on DVD. Something that } would present the technology in an organized fashion and be } comprehensive enough to put a student in a position to begin work in the } area. If such a project is undertaken, Steve Barlow and Howard Berg } have agreed to work with me on coordinating it. } First, we would like to hear from the community on whether or not } they think this is a good idea. Second we would be looking for } volunteers who might be willing to record a lesson with supporting } demonstrations and other visual representations that could flesh out the } coverage. We don't want just a talking head. We would also be looking } for folks who would be willing to share the syllabi from their courses } so that we might determine how to go about organizing such a thing. } Any and all feedback is welcome. } } Regarsd to all, } Greg } } } } } -- } Gregory W. Erdos, Ph.D. } Assistant Director, Biotechnology Program } Scientific Director, Electron Microscopy } P.O. Box 118525 } 217 Carr Hall } University of Florida } Gainesville, FL 32611 } Phone: 352-392-1295 } Fax: 352 846 0251 } } } } ==============================Original Headers============================== } 7, 21 -- From gwe-at-ufl.edu Thu Sep 8 14:02:51 2005 } 7, 21 -- Received: from smtp.ufl.edu (sp42en1.nerdc.ufl.edu [128.227.74.42]) } 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j88J2o5c032635 } 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 14:02:51 -0500 } 7, 21 -- Received: from [127.0.0.1] (pc2524c.dhcp.clas.ufl.edu [128.227.60.197]) } 7, 21 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j88J2lEF127796 } 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 15:02:49 -0400 } 7, 21 -- Message-ID: {43208ADE.6050104-at-ufl.edu} } 7, 21 -- Date: Thu, 08 Sep 2005 15:02:54 -0400 } 7, 21 -- From: Greg Erdos {gwe-at-ufl.edu} } 7, 21 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) } 7, 21 -- X-Accept-Language: en-us, en } 7, 21 -- MIME-Version: 1.0 } 7, 21 -- To: microscopy-at-microscopy.com } 7, 21 -- Subject: Tutorials } 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 21 -- Content-Transfer-Encoding: 7bit } 7, 21 -- X-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 } 7, 21 -- X-UFL-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 } 7, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) } 7, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 23 -- From walck-at-southbaytech.com Thu Sep 8 18:16:27 2005 5, 23 -- Received: from smtp05.safesecureweb.com (smtp05.safesecureweb.com [209.41.179.8]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j88NGQ7f012740 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 18:16:27 -0500 5, 23 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 5, 23 -- by smtp05.safesecureweb.com (Postfix) with ESMTP id BC56B5541DB 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 19:16:23 -0400 (EDT) 5, 23 -- Received: from mail15.safesecureweb.com (unknown [192.168.2.180]) 5, 23 -- by smtp05.safesecureweb.com (Postfix) with ESMTP id 917135540DF 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 19:16:19 -0400 (EDT) 5, 23 -- MIME-Version: 1.0 5, 23 -- Date: Thu, 8 Sep 2005 19:14:36 -0400 5, 23 -- Content-Type: text/plain; 5, 23 -- charset=iso-8859-1 5, 23 -- Subject: re: [Microscopy] Tutorials 5, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 5, 23 -- Reply-To: Walck-at-southbaytech.com 5, 23 -- To: {Microscopy-at-microscopy.com} 5, 23 -- Cc: 5, 23 -- Message-ID: {02611d82b84549f8ae444fc7b3f0d444-at-southbaytech.com} 5, 23 -- X-Virus-Scanned: by amavisd-new-20030616-p10 at safesecureweb.com 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j88NGQ7f012740 ==============================End of - Headers==============================
Skage Hem wrote: ===================================================== Anyone know a good source for Silicate standards for EDS/WDS analysis ?
Any advice is greatly appriciated. ===================================================== SPI Supplies offers a number of silicate containing standards, from both the SPI Supplies and also the Charles M. Taylor standards collection. Go to URL http://www.2spi.com/catalog/standards/aweb/index.html and on the Periodic Table of the Elements, click on Si and then you will get a display of all the silicon containing standards including of course those that are silicates.
These standard items are all all stock and can be offered as both loose standards or mounted in a finished "block" complete with electron beam labeling, Faraday cup and instruction manual.
Disclaimer: SPI Supplies is a supplier of standards for microanalysis.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 11, 23 -- From cgarber-at-2spi.com Thu Sep 8 20:11:10 2005 11, 23 -- Received: from mailrelay.eurospot.com (mailrelay.eurospot.com [62.39.81.196]) 11, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j891BA9q022219 11, 23 -- for {microscopy-at-msa.microscopy.com} ; Thu, 8 Sep 2005 20:11:10 -0500 11, 23 -- Received: from ibm1x23g2abfyg (85-18-163-242.ip.fastwebnet.it [85.18.163.242]) 11, 23 -- by mailrelay.eurospot.com (Postfix) with SMTP 11, 23 -- id B3D2D286C1D; Fri, 9 Sep 2005 03:11:08 +0200 (CEST) 11, 23 -- Message-ID: {016e01c5b4db$5b9eabd0$a433a8c0-at-ibm1x23g2abfyg} 11, 23 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 11, 23 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 11, 23 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 11, 23 -- Subject: Availability of silicate standards for microanalysis 11, 23 -- Date: Thu, 8 Sep 2005 21:11:07 -0400 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; 11, 23 -- format=flowed; 11, 23 -- charset="Windows-1252"; 11, 23 -- reply-type=original 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- X-Priority: 3 11, 23 -- X-MSMail-Priority: Normal 11, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 11, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lahec-at-wcoil.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, September 8, 2005 at 15:32:34 ---------------------------------------------------------------------------
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pgan-at-ap.ansell.com) from http://microscopy.com/MLFormMail.html on Thursday, September 8, 2005 at 18:48:33 ---------------------------------------------------------------------------
Email: pgan-at-ap.ansell.com Name: Phay Fang Gan
Organization: Ansell
Title-Subject: [Filtered] Shelf Life
Question: May I know the shelf life of the conductive silver paint and carbon paint ?
Image acquisition, processing and analysis in biomedical research
This symposium is organised by Daniel Zicha (Cancer Research UK) and Alan Boyde (Queen Mary University of London). The scientific sessions will take place in the Dent Room, Student Union Building, 43-46 Huntley Street, London WC1. or if the number of attendees is too great, in the Anatomy Department, University College London, Gower St. Late registration is still available for £10 (including refreshments) but intending registrants should email the Programme Secretary (jonathan.bennett-at-hyms.ac.uk) to indicate their intention of attending.
Alan Boyde, Biophysics Section, Centre for Oral Growth and Development, QMUL, Dental Institute, New Rd., London E1 1BB, UK {a.boyde-at-qmul.ac.uk}
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I can't answer about graphite paint, but I am still using the same bottle of colloidal silver after 10 years. I'm a TEM (AFM,XRD,FIB...) man really so I only use it to mount samples for SEM once in a while. Even if it's completely dried out I find that adding a bit of the relevant solvent (4-Metyl-pentan-2-one, usually) and a buzz in an ultrasonic bath for an hour makes it as good as new. So I would call the shelf life "unlimited". The main problem is getting the damn lid off the bottle..
-----Original Message----- X-from: pgan-at-ap.ansell.com [mailto:pgan-at-ap.ansell.com] Sent: 09 September 2005 08:57 To: Richard Beanland
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pgan-at-ap.ansell.com) from http://microscopy.com/MLFormMail.html on Thursday, September 8, 2005 at 18:48:33 ---------------------------------------------------------------------------
Email: pgan-at-ap.ansell.com Name: Phay Fang Gan
Organization: Ansell
Title-Subject: [Filtered] Shelf Life
Question: May I know the shelf life of the conductive silver paint and carbon paint ?
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==============================Original Headers============================== 19, 29 -- From richard.beanland-at-bookham.com Fri Sep 9 04:26:03 2005 19, 29 -- Received: from mail72.messagelabs.com (mail72.messagelabs.com [193.109.255.147]) 19, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j899Q2nD004468 19, 29 -- for {microscopy-at-microscopy.com} ; Fri, 9 Sep 2005 04:26:03 -0500 19, 29 -- X-VirusChecked: Checked 19, 29 -- X-Env-Sender: richard.beanland-at-bookham.com 19, 29 -- X-Msg-Ref: server-8.tower-72.messagelabs.com!1126257961!26564132!1 19, 29 -- X-StarScan-Version: 5.4.15; banners=bookham.com,-,- 19, 29 -- X-Originating-IP: [213.249.209.179] 19, 29 -- Received: (qmail 25582 invoked from network); 9 Sep 2005 09:26:01 -0000 19, 29 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 19, 29 -- by server-8.tower-72.messagelabs.com with SMTP; 9 Sep 2005 09:26:01 -0000 19, 29 -- Content-class: urn:content-classes:message 19, 29 -- MIME-Version: 1.0 19, 29 -- Content-Type: text/plain; 19, 29 -- charset="iso-8859-1" 19, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 19, 29 -- Subject: RE: [Microscopy] viaWWW: shelf life of the conductive paint 19, 29 -- Date: Fri, 9 Sep 2005 10:26:00 +0100 19, 29 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBC8D-at-cas-smx-01.caswell1.europe.bkhm.net} 19, 29 -- X-MS-Has-Attach: 19, 29 -- X-MS-TNEF-Correlator: 19, 29 -- Thread-Topic: [Microscopy] viaWWW: shelf life of the conductive paint 19, 29 -- Thread-Index: AcW1FDnVPGD9ssH0Q2am1c+UkXyFiQACuj5Q 19, 29 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 19, 29 -- To: {pgan-at-ap.ansell.com} 19, 29 -- Cc: {microscopy-at-microscopy.com} 19, 29 -- Content-Transfer-Encoding: 8bit 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j899Q2nD004468 ==============================End of - Headers==============================
I would say much the same as Richard Beanland but about carbon paint. We have had jars sitting from a large order sitting on the shelf for years. They have always been fine when I open a new one.
Our big problem is getting users to put the caps back on tightly. A couple times a month I go around our lab and check the opened bottles and add propanol to bring them back to the original consistency. Time in the sonic batch would probably help, but it will just dry out again. I usually give it a good shake, let it sit for a while and shake it again. Even with that, I usually rarely get a chance to through a bottle away because it's empty. Usually, I find one that has dried out too much before I get to it and figure it is not worth the hassle to try to rejuvenate it.
Warren Straszheim Iowa State University
At 02:56 AM 09/09/05, you wrote:
} Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (pgan-at-ap.ansell.com) from } http://microscopy.com/MLFormMail.html on Thursday, September 8, 2005 } at 18:48:33 } --------------------------------------------------------------------------- } } Email: pgan-at-ap.ansell.com } Name: Phay Fang Gan } } Organization: Ansell } } Title-Subject: [Filtered] Shelf Life } } Question: May I know the shelf life of the conductive silver paint } and carbon paint ? } } Thanks
==============================Original Headers============================== 6, 21 -- From wesaia-at-iastate.edu Fri Sep 9 08:33:40 2005 6, 21 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89DXde0011621 6, 21 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 08:33:40 -0500 6, 21 -- Received: from mailout-2.iastate.edu (mailout-2.iastate.edu [129.186.140.2]) 6, 21 -- by mailhub-3.iastate.edu (8.12.10/8.12.10) with SMTP id j89DXRiI028491; 6, 21 -- Fri, 9 Sep 2005 08:33:27 -0500 6, 21 -- Received: from strasz.marl.iastate.edu(129.186.227.11) by mailout-2.iastate.edu via csmap 6, 21 -- id 3c1847f2_2138_11da_99a9_003048290bef_24663; 6, 21 -- Fri, 09 Sep 2005 08:47:17 -0500 (CDT) 6, 21 -- Message-Id: {6.2.0.14.2.20050909082624.02ae3cc8-at-wesaia.mail.iastate.edu} 6, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 6, 21 -- Date: Fri, 09 Sep 2005 08:31:41 -0500 6, 21 -- To: MSA listserver {Microscopy-at-msa.microscopy.com} 6, 21 -- From: Warren E Straszheim {wesaia-at-iastate.edu} 6, 21 -- Subject: Re: [Microscopy] viaWWW: shelf life of the conductive paint 6, 21 -- Cc: pgan-at-ap.ansell.com 6, 21 -- In-Reply-To: {200509090756.j897u0c0012103-at-ns.microscopy.com} 6, 21 -- References: {200509090756.j897u0c0012103-at-ns.microscopy.com} 6, 21 -- Mime-Version: 1.0 6, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
A question for the list: I am looking for some (cheap/easy to use) 3D reconstruction software to make 3D models from CT slices. I'm not looking for anything to fancy. I'm looking at CT data from extant crab claws and need to model an internal structure that is more dense, and show it in relation to the exterior of the claw. The two programs I have looked at are Surfdriver (now only for PC) and VGStudio Max. Does anyone have any other software options I should be looking at? I'm hoping the software would work on the Mac, but if I had to I could find a PC. Many thanks in advance. -David -- David A. Waugh Kent State University Department of Geology Kent, Ohio 44242 dwaugh-at-kent.edu Http://www.personal.kent.edu/~dwaugh/
==============================Original Headers============================== 2, 30 -- From dwaugh-at-kent.edu Fri Sep 9 09:07:43 2005 2, 30 -- Received: from smtp.kent.edu (vscan.kent.edu [131.123.246.3]) 2, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89E7hBD027107 2, 30 -- for {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 09:07:43 -0500 2, 30 -- Received: from localhost (localhost [127.0.0.1]) 2, 30 -- by smtp.kent.edu (Postfix) with ESMTP id F1E94763094 2, 30 -- for {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 09:24:46 -0400 (EDT) 2, 30 -- Received: from smtp.kent.edu ([127.0.0.1]) 2, 30 -- by localhost (smtp.kent.edu [127.0.0.1]) (amavisd-new, port 10024) with ESMTP 2, 30 -- id 20446-04 for {Microscopy-at-Microscopy.com} ; 2, 30 -- Fri, 9 Sep 2005 09:24:45 -0400 (EDT) 2, 30 -- Received: from kent.edu (flash03.uis.kent.edu [131.123.250.93]) 2, 30 -- by smtp.kent.edu (Postfix) with ESMTP id 327CB76375D 2, 30 -- for {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 09:21:30 -0400 (EDT) 2, 30 -- Received: from [131.123.229.223] by flashmail.kent.edu (mshttpd); Fri, 2, 30 -- 09 Sep 2005 10:04:26 -0400 2, 30 -- From: {dwaugh-at-kent.edu} 2, 30 -- To: Microscopy-at-Microscopy.com 2, 30 -- Message-ID: {1dcc3881dcaefd.1dcaefd1dcc388-at-kent.edu} 2, 30 -- Date: Fri, 09 Sep 2005 10:04:26 -0400 2, 30 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14 2004) 2, 30 -- MIME-Version: 1.0 2, 30 -- Content-Language: en 2, 30 -- Subject: 3D CT Reconstruction Software 2, 30 -- X-Accept-Language: en 2, 30 -- Priority: normal 2, 30 -- Content-Type: text/plain; charset=us-ascii 2, 30 -- Content-Disposition: inline 2, 30 -- Content-Transfer-Encoding: 7bit 2, 30 -- X-Virus-Scanned: amavisd-new at kent.edu ==============================End of - Headers==============================
Depending on the sophistication of the reconstruction you need, you should check out ImageJ, which is available free at: http://rsb.info.nih.gov/ij/. The program is written in Java, and will run on virtually any platform.
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } A question for the list: I am looking for some (cheap/easy to use) 3D } reconstruction software to make 3D models from CT slices. I'm not } looking for anything to fancy. I'm looking at CT data from extant crab } claws and need to model an internal structure that is more dense, and } show it in relation to the exterior of the claw. The two programs I } have looked at are Surfdriver (now only for PC) and VGStudio Max. Does } anyone have any other software options I should be looking at? I'm } hoping the software would work on the Mac, but if I had to I could } find a PC. Many thanks in advance. -David -- David A. Waugh Kent State } University Department of Geology Kent, Ohio 44242 dwaugh-at-kent.edu } Http://www.personal.kent.edu/~dwaugh/ } } ==============================Original } Headers============================== 2, 30 -- From dwaugh-at-kent.edu } Fri Sep 9 09:07:43 2005 2, 30 -- Received: from smtp.kent.edu } (vscan.kent.edu [131.123.246.3]) 2, 30 -- by ns.microscopy.com } (8.12.11/8.12.8) with ESMTP id j89E7hBD027107 2, 30 -- for } {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 09:07:43 -0500 2, 30 -- } Received: from localhost (localhost [127.0.0.1]) 2, 30 -- by } smtp.kent.edu (Postfix) with ESMTP id F1E94763094 2, 30 -- for } {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 09:24:46 -0400 (EDT) 2, } 30 -- Received: from smtp.kent.edu ([127.0.0.1]) 2, 30 -- by } localhost (smtp.kent.edu [127.0.0.1]) (amavisd-new, port 10024) with } ESMTP 2, 30 -- id 20446-04 for {Microscopy-at-Microscopy.com} ; 2, 30 -- } Fri, 9 Sep 2005 09:24:45 -0400 (EDT) 2, 30 -- Received: from kent.edu } (flash03.uis.kent.edu [131.123.250.93]) 2, 30 -- by smtp.kent.edu } (Postfix) with ESMTP id 327CB76375D 2, 30 -- for } {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 09:21:30 -0400 (EDT) 2, } 30 -- Received: from [131.123.229.223] by flashmail.kent.edu } (mshttpd); Fri, 2, 30 -- 09 Sep 2005 10:04:26 -0400 2, 30 -- From: } {dwaugh-at-kent.edu} 2, 30 -- To: Microscopy-at-Microscopy.com 2, 30 -- } Message-ID: {1dcc3881dcaefd.1dcaefd1dcc388-at-kent.edu} 2, 30 -- Date: } Fri, 09 Sep 2005 10:04:26 -0400 2, 30 -- X-Mailer: iPlanet Messenger } Express 5.2 Patch 2 (built Jul 14 2004) 2, 30 -- MIME-Version: 1.0 2, } 30 -- Content-Language: en 2, 30 -- Subject: 3D CT Reconstruction } Software 2, 30 -- X-Accept-Language: en 2, 30 -- Priority: normal 2, } 30 -- Content-Type: text/plain; charset=us-ascii 2, 30 -- } Content-Disposition: inline 2, 30 -- Content-Transfer-Encoding: 7bit } 2, 30 -- X-Virus-Scanned: amavisd-new at kent.edu } ==============================End of - } Headers==============================
Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 6, 20 -- From jbs-at-temple.edu Fri Sep 9 09:15:05 2005 6, 20 -- Received: from imp1.temple.edu (imp1.ocis.temple.edu [155.247.166.81]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89EF5tn004560 6, 20 -- for {microscopy-at-microscopy.com} ; Fri, 9 Sep 2005 09:15:05 -0500 6, 20 -- Received: from JBS (jbs.bio.temple.edu [155.247.98.40]) 6, 20 -- by imp1.temple.edu (8.12.3/8.11.3/SuSE Linux 8.11.1-0.5) with ESMTP id j89EEKd0031375; 6, 20 -- Fri, 9 Sep 2005 10:14:44 -0400 6, 20 -- From: "Joel Sheffield" {jbs-at-temple.edu} 6, 20 -- To: dwaugh-at-kent.edu, dwaugh-at-kent.edu, microscopy-at-microscopy.com 6, 20 -- Date: Fri, 09 Sep 2005 10:14:26 -0400 6, 20 -- MIME-Version: 1.0 6, 20 -- Subject: Re: [Microscopy] 3D CT Reconstruction Software 6, 20 -- Reply-to: jbs-at-temple.edu 6, 20 -- Message-ID: {43216082.8097.32FEAB3D-at-localhost} 6, 20 -- Priority: normal 6, 20 -- In-reply-to: {200509091407.j89E7whd027378-at-ns.microscopy.com} 6, 20 -- X-mailer: Pegasus Mail for Windows (4.21c) 6, 20 -- Content-type: text/plain; charset=US-ASCII 6, 20 -- Content-transfer-encoding: 7BIT 6, 20 -- Content-description: Mail message body ==============================End of - Headers==============================
OK people. I've tried to send this twice already, and had some problems which do not appear evident to me. But will try again.
I would like to try and get a thread going on the subject of reduced osmium fixation. Not so much on the ‘what I’ve used’ form as the ‘what is the difference, what is the value, what is the mechanism’ form. Some hypothetical, perhaps our more chemically inclined can provide some factual information on chemical reactions, and some ‘what protocols exist’ form. Gee, I guess that last point does get into the what 'I’ve used' form, doesn’t it. OK, include the 'what I've used'.
The source of interest which has given rise to this is a question a student asked the other day. This person had ferrocyanide (Fe4+) and wanted to know if it were possible to use it in place of ferricyanide (Fe3+). Since my procedure called for ferricyanide, and I had some, I gave it to them and they went away happy. At least I think they were happy, I never can really tell about students. Then there was the listing from Richard Berry in Australia, and Geoff McAuliff's response which also raised Osmium-ferricyanide.
Unfortunately, this has all started me thinking - always a dangerous thing. So, I checked my copy of Hayat’s Fixation for Electron Microscopy [sorry Phil, some of us do have it ;-)]. The results were quite interesting.
Hayat mentioned procedures for both ferri- and ferrocyanide. Went to his original references, read those i could get my hands on readily (our library has taken to storing some older issues of journals). I won’t get into a long re-hash of what is there. Briefly: 1.) The original hypothesis by Elbers was that fixation with ferricyanide/lead as a step between glut and osmium, could help stabilize phospholipids. Subsequently it was used to stabilize surfactants. Maybe stabilize surfactants - there is mixed data on that. The key seemed to be the presence of the Pb, not the Fe. 2.) There was a ferricyanide report that used 1.6% ferricyanide, 1% Osmium. Note, this is 2x the concentration of ferricyanide I use now. Hayat didn't really say what the advantage was supposed to be, though.. 3.) Karnovsky’s report to the 11th (or 14th) meeting of the Am Soc. for Cell Biology in 1970, and the Russell & Burguet work from 1977 were discussed, but the roles in membrane fixation, or mechanism, for that matter, were not. This is also covered in Bozzolo and Russell under membrane fixation. 4.) de Bruijn and Den Breejen’s work (1975) which showed no difference in ferricyanide and ferrocyanide reduced Os in terms of subsequent staining of glycogen was referenced, but not discussed.
Next, I checked the catalogues to see what the EM suppliers made available. That's always a good indicator as to what people are using. Some suppliers had ferricyanide, some didn't. And i found none with ferrocyanide. Some of our regular suppliers of non EM lab supplies, chemicals, etc, do sell both, and as 10% solutions, which makes using it real easy!!!
So, for the thread. Is there a difference between ferri- and ferrocyanide. If there are any differences, why? Is the operative agent iron, as a reducer of osmium from VIII to VI, or is it a mordant effect of the cyanide moieties - as suggested by some? What concentration of ferri/ferrocyanide should we use, 0.8%, 1.5%, or even 2.5% as recommeded by Russell and Burguet?
Today, my only thought is that K4Fe(CN)6 would reduce the Os from octavalent to hexavalent more quickly than K3Fe(CN)6, but I doubt it would make a practical difference in the fixative.
Any takers on seeing if we can educate ourselves on this. Get a good, profitable discussion on line?
paul
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
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We are looking into the possibility of replacing our film cameras with Imaging Plates. I am aware of the Fuji and Ditabis systems. Are there any other systems available? (vendors welcome to respond!)
I would also appreciate any feedback concerning the use of the IP systems, both concerning the particular vendor/system and IP units in general. It is probably best to keep the feedback off-line. I can summarize to the list later.
Thanks, Henk
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 7, 24 -- From colijn.1-at-osu.edu Fri Sep 9 10:21:16 2005 7, 24 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 7, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89FLGFC029373 7, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Sep 2005 10:21:16 -0500 7, 24 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 7, 24 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x5 #31056) 7, 24 -- id {01LSTQ9DE0HS9HA5CK-at-er6s1.eng.ohio-state.edu} for 7, 24 -- Microscopy-at-microscopy.com; Fri, 09 Sep 2005 11:21:15 -0400 (EDT) 7, 24 -- Received: from HOC1.er6.eng.ohio-state.edu 7, 24 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.eng.ohio-state.edu 7, 24 -- (PMDF V6.2-1x5 #31056) 7, 24 -- with ESMTPA id {01LSTQ9CWRTQ9HKK8N-at-er6s1.eng.ohio-state.edu} for 7, 24 -- Microscopy-at-microscopy.com; Fri, 09 Sep 2005 11:21:15 -0400 (EDT) 7, 24 -- Date: Fri, 09 Sep 2005 11:23:59 -0400 7, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 7, 24 -- Subject: Imaging Plates 7, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 7, 24 -- X-Sender: colijn-at-mail.er6.eng.ohio-state.edu 7, 24 -- To: Microscopy-at-microscopy.com 7, 24 -- Message-id: {6.1.0.6.2.20050909080858.02cc9bd8-at-mail.er6.eng.ohio-state.edu} 7, 24 -- MIME-version: 1.0 7, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.0.6 7, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed 7, 24 -- X-Env-From: auth/colijn.1-at-osu.edu ==============================End of - Headers==============================
I subdivide a new bottle of colloidal graphite into several small vials (usually liquid scintilation vials) and cap them tightly. This way, as the vial in use dries out (or gets left uncapped), I don't lose the entire stock. --Jan Factor
--------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College, State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
pgan-at-ap.ansell.com wrote:
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==============================Original Headers============================== 6, 20 -- From jfactor-at-ns.purchase.edu Fri Sep 9 10:34:25 2005 6, 20 -- Received: from zephyr.ns.purchase.edu (purvid.ns.purchase.edu [199.79.168.193]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89FYNtL005413 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Sep 2005 10:34:24 -0500 6, 20 -- Received: from ns.purchase.edu ([10.52.0.64]) 6, 20 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0) with ESMTP id j89FaYX28476; 6, 20 -- Fri, 9 Sep 2005 11:36:34 -0400 6, 20 -- Message-ID: {4321AB74.30604-at-ns.purchase.edu} 6, 20 -- Date: Fri, 09 Sep 2005 11:34:12 -0400 6, 20 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.6) Gecko/20040113 6, 20 -- X-Accept-Language: en-us, en 6, 20 -- MIME-Version: 1.0 6, 20 -- To: pgan-at-ap.ansell.com 6, 20 -- CC: "'Microscopy list'" {Microscopy-at-MSA.Microscopy.Com} 6, 20 -- Subject: Re: [Microscopy] viaWWW: shelf life of the conductive paint 6, 20 -- References: {200509090756.j897u2gO012198-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200509090756.j897u2gO012198-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We tell our customers one year unopened, six months after being opened, however.we know of people keeping and using much longer than that. If you have any further questions or want to discuss this with someone, our chemist Dr. Charles Duvic would be happy to speak with you. Please call or e-mail him at the numbers listed below.
JD Arnott
Ladd Research 83 Holly Court Williston, VT 05495
On-line Catalog: http://www.laddresearch.com
tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US) fax: 1-802-660-8859 e-mail: ladres-at-att.net
******************** Disclaimer: Ladd Research sells Supplies for microscopy such as conducting paints listed in this e-mail. ********************
----- Original Message ----- X-from: {pgan-at-ap.ansell.com} To: {ladres-at-worldnet.att.net} Sent: Friday, September 09, 2005 3:59 AM
One thing that seems to help prolong the usability of carbon paint is to shake the bottle *after* you're through using it. This helps wash the semi-dried material at the neck back into solution, so you don't get as much dried up gunk in the neck of the bottle after repeated use. This is especially true if the cap has a brush built in, and you use the edge of the bottle to slurp off the excess before applying to stubs. Just make sure the bottle is tightly closed (speaking from experience).
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
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==============================Original Headers============================== 10, 21 -- From jehrman-at-mta.ca Fri Sep 9 11:00:39 2005 10, 21 -- Received: from mailserv.mta.ca (mailserv.mta.ca [138.73.1.1]) 10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89G0dHa022525 10, 21 -- for {Microscopy-at-MSA.Microscopy.com} ; Fri, 9 Sep 2005 11:00:39 -0500 10, 21 -- Received: from host-22-245.mta.ca ([138.73.22.245]) 10, 21 -- by mailserv.mta.ca with esmtp (Exim 4.52) 10, 21 -- id 1EDlIq-0003MH-QH; Fri, 09 Sep 2005 13:00:38 -0300 10, 21 -- Message-ID: {4321B1A4.1020708-at-mta.ca} 10, 21 -- Date: Fri, 09 Sep 2005 13:00:36 -0300 10, 21 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 10, 21 -- Organization: Mount Allison University 10, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 10, 21 -- X-Accept-Language: en-us, en 10, 21 -- MIME-Version: 1.0 10, 21 -- To: wesaia-at-iastate.edu 10, 21 -- CC: Microscopy Listserv {Microscopy-at-MSA.Microscopy.com} 10, 21 -- Subject: Re: [Microscopy] Re: viaWWW: shelf life of the conductive paint 10, 21 -- References: {200509091334.j89DYBF6012745-at-ns.microscopy.com} 10, 21 -- In-Reply-To: {200509091334.j89DYBF6012745-at-ns.microscopy.com} 10, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear David, Take a look at Osirix. http://homepage.mac.com/rossetantoine/osirix/
Regards, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires Windows 95 or better", so I bought a Macintosh. ************************************************************************ ******
On Sep 9, 2005, at 7:09 AM, dwaugh-at-kent.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } A question for the list: I am looking for some (cheap/easy to use) 3D } reconstruction software to make 3D models from CT slices. I'm not } looking for anything to fancy. I'm looking at CT data from extant } crab claws and need to model an internal structure that is more } dense, and show it in relation to the exterior of the claw. The two } programs I have looked at are Surfdriver (now only for PC) and } VGStudio Max. Does anyone have any other software options I should be } looking at? I'm hoping the software would work on the Mac, but if I } had to I could find a PC. Many thanks in advance. } -David } -- } David A. Waugh } Kent State University } Department of Geology } Kent, Ohio 44242 } dwaugh-at-kent.edu } Http://www.personal.kent.edu/~dwaugh/ } } ==============================Original } Headers============================== } 2, 30 -- From dwaugh-at-kent.edu Fri Sep 9 09:07:43 2005 } 2, 30 -- Received: from smtp.kent.edu (vscan.kent.edu [131.123.246.3]) } 2, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j89E7hBD027107 } 2, 30 -- for {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 } 09:07:43 -0500 } 2, 30 -- Received: from localhost (localhost [127.0.0.1]) } 2, 30 -- by smtp.kent.edu (Postfix) with ESMTP id F1E94763094 } 2, 30 -- for {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 } 09:24:46 -0400 (EDT) } 2, 30 -- Received: from smtp.kent.edu ([127.0.0.1]) } 2, 30 -- by localhost (smtp.kent.edu [127.0.0.1]) (amavisd-new, } port 10024) with ESMTP } 2, 30 -- id 20446-04 for {Microscopy-at-Microscopy.com} ; } 2, 30 -- Fri, 9 Sep 2005 09:24:45 -0400 (EDT) } 2, 30 -- Received: from kent.edu (flash03.uis.kent.edu } [131.123.250.93]) } 2, 30 -- by smtp.kent.edu (Postfix) with ESMTP id 327CB76375D } 2, 30 -- for {Microscopy-at-Microscopy.com} ; Fri, 9 Sep 2005 } 09:21:30 -0400 (EDT) } 2, 30 -- Received: from [131.123.229.223] by flashmail.kent.edu } (mshttpd); Fri, } 2, 30 -- 09 Sep 2005 10:04:26 -0400 } 2, 30 -- From: {dwaugh-at-kent.edu} } 2, 30 -- To: Microscopy-at-Microscopy.com } 2, 30 -- Message-ID: {1dcc3881dcaefd.1dcaefd1dcc388-at-kent.edu} } 2, 30 -- Date: Fri, 09 Sep 2005 10:04:26 -0400 } 2, 30 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul } 14 2004) } 2, 30 -- MIME-Version: 1.0 } 2, 30 -- Content-Language: en } 2, 30 -- Subject: 3D CT Reconstruction Software } 2, 30 -- X-Accept-Language: en } 2, 30 -- Priority: normal } 2, 30 -- Content-Type: text/plain; charset=us-ascii } 2, 30 -- Content-Disposition: inline } 2, 30 -- Content-Transfer-Encoding: 7bit } 2, 30 -- X-Virus-Scanned: amavisd-new at kent.edu } ==============================End of - } Headers============================== }
Does anyone have a source for colloidal gold (preferably 10nm) conjugated to human IgE? We are trying to identify the source of an allergen so the right conjugate is rather critical.
Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 6, 19 -- From dsherman-at-purdue.edu Fri Sep 9 12:51:27 2005 6, 19 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89HpQp7008675 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 9 Sep 2005 12:51:26 -0500 6, 19 -- Received: from [128.210.121.247] (lsmf07.btny.purdue.edu [128.210.121.247]) 6, 19 -- by mailhub129.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j89HpQDv022530 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 9 Sep 2005 12:51:26 -0500 6, 19 -- User-Agent: Microsoft-Entourage/11.1.0.040913 6, 19 -- Date: Fri, 09 Sep 2005 12:51:24 -0500 6, 19 -- Subject: Need IgE conjugate 6, 19 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 19 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 19 -- Message-ID: {BF4735CC.879E%dsherman-at-purdue.edu} 6, 19 -- Mime-version: 1.0 6, 19 -- Content-type: text/plain; 6, 19 -- charset="US-ASCII" 6, 19 -- Content-transfer-encoding: 7bit 6, 19 -- X-PMX-Version: 4.7.1.128075 6, 19 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
An impressive mold, growing in an arsenical compound. I suspect there is no less labor intensive method. I certainly never found an easier method when I was doing amphipods. The best way to store the EM specimens is to go ahead and process and embed them. Once they're in plastic, they'll last for years. With no mold. The good fixation is more important than less labor.
Phil
} I need to clarify my fixation question. First, I am trying to fix marine } amphipods for both light and TEM. Currently I am fixing them in 2.5% } Gluteraldehyde and 2.5% Formaldehyde in sodium cacodylate buffer and } bisecting the animal and placing half in 70% EtOH for light microscopy } and the other half in cacodylic buffer. I then process the animals for } light and if the pathologist sees anything interesting I will have the } other half of the animal fixed for any em work. I am not doing any immuno. } } The fixation is fine, but is there a less labor intensive way? I don't } like storing my tissue in the buffer because it molds after about 8 months. } } I appreciate all of your questions and I look forward to your comments. } } Sue
==============================Original Headers============================== 5, 28 -- From peoshel-at-wisc.edu Fri Sep 9 13:36:25 2005 5, 28 -- Received: from smtp7.wiscmail.wisc.edu (hagen.doit.wisc.edu [144.92.197.163]) 5, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89IaPTj017683 5, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Sep 2005 13:36:25 -0500 5, 28 -- Received: from avs-daemon.smtp7.wiscmail.wisc.edu by smtp7.wiscmail.wisc.edu 5, 28 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 5, 28 -- id {0IMK0030MBOOJO-at-smtp7.wiscmail.wisc.edu} for microscopy-at-microscopy.com; 5, 28 -- Fri, 09 Sep 2005 13:36:24 -0500 (CDT) 5, 28 -- Received: from [128.104.216.125] 5, 28 -- (ras-c5800-3-216-125.dialup.wisc.edu [128.104.216.125]) 5, 28 -- by smtp7.wiscmail.wisc.edu 5, 28 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 5, 28 -- with ESMTPSA id {0IMK00LYRBNMAT-at-smtp7.wiscmail.wisc.edu} for 5, 28 -- microscopy-at-microscopy.com; Fri, 09 Sep 2005 13:36:21 -0500 (CDT) 5, 28 -- Date: Fri, 09 Sep 2005 13:35:42 -0500 5, 28 -- From: Philip Oshel {peoshel-at-wisc.edu} 5, 28 -- Subject: Re: [Microscopy] Clarification of Marine TEM fixative 5, 28 -- In-reply-to: {200509071817.j87IHuiR023436-at-ns.microscopy.com} 5, 28 -- X-Sender: peoshel-at-wiscmail.wisc.edu 5, 28 -- To: microscopy-at-microscopy.com 5, 28 -- Message-id: {p06110409bf4785461449-at-[128.104.217.170]} 5, 28 -- MIME-version: 1.0 5, 28 -- Content-type: text/plain; format=flowed; charset=us-ascii 5, 28 -- Content-transfer-encoding: 7BIT 5, 28 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=128.104.216.125 5, 28 -- X-Spam-PmxInfo: Server=avs-7, Version=5.0.3.165339, Antispam-Engine: 2.1.0.0, 5, 28 -- Antispam-Data: 2005.9.9.20, SenderIP=128.104.216.125 5, 28 -- References: {200509071817.j87IHuiR023436-at-ns.microscopy.com} ==============================End of - Headers==============================
A colleague, who is experiencing specimen damage in the TEM, inquired if anyone knew the temperature generated on the specimen by the electron beam. I realize that there are a lot of variables here, but even a range of temperatures would be useful.
Here are his operating parameters:
kV = 100 spot size = 2 micrometer (specimen was examined at approximately 2 micrometer spot size) specimen = ceramic containing metal particles (Ti) magnification = 100K to 600K electron gun = LaB6 vacuum = turbo pumped to 10-5 Pa substrate that specimen was mounted on = Formvar/carbon coated grid
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu ##############################################################
==============================Original Headers============================== 6, 16 -- From bozzola-at-siu.edu Fri Sep 9 13:51:34 2005 6, 16 -- Received: from abbmta1.siu.edu (abbmta1.siu.edu [131.230.254.205]) 6, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89IpYTY026191 6, 16 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 13:51:34 -0500 6, 16 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 6, 16 -- by abbmta1.siu.edu (Switch-3.1.0/Switch-3.1.0) with ESMTP id j89IpWlh021857 6, 16 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 13:51:34 -0500 (CDT) 6, 16 -- Mime-Version: 1.0 6, 16 -- X-Sender: bozzola-at-saluki-mail.siu.edu 6, 16 -- Message-Id: {p06110432bf4789710b4a-at-[131.230.177.142]} 6, 16 -- Date: Fri, 9 Sep 2005 13:51:43 -0500 6, 16 -- To: Microscopy-at-msa.microscopy.com 6, 16 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 6, 16 -- Subject: temperature of 100 kV beam 6, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 16 -- X-MASF: 0.00% ==============================End of - Headers==============================
On Sep 9, 2005, at 11:51 AM, bozzola-at-siu.edu wrote:
} A colleague, who is experiencing specimen damage in the TEM, inquired } if anyone knew the temperature generated on the specimen by the } electron beam. I realize that there are a lot of variables here, but } even a range of temperatures would be useful. } } Here are his operating parameters: } } } kV = 100 } spot size = 2 micrometer (specimen was examined at approximately 2 } micrometer spot size) } specimen = ceramic containing metal particles (Ti) } magnification = 100K to 600K } electron gun = LaB6 } vacuum = turbo pumped to 10-5 Pa } substrate that specimen was mounted on = Formvar/carbon coated grid } Dear John, Unfortunately, I don't have one of my most useful references with me to get an essential parameter, but the general method of doing this calculation is to use the stopping power of the material to determine the energy deposited into the specimen, then calculate the temperature increase from the heat capacity and account for conduction and radiation of heat. At steady state, the heat in, which is the stopping power, dE/dx, in units of joules/meter times the electron beam current times the specimen thickness, must equal the sum of conduction (assume a disk at one temperature surrounded by an infinite amount of the material at ambient temperature, plug in the conductivity, the temperature difference, and the area across which the heat is conducted, which is the circumference of the beam spot times the thickness of the specimen) and radiation, which is equal to T^4 (on the Kelvin scale) times the area of the beam times the Stephan-Boltzman constant. The stopping power can be set equal to the sum of stopping powers for each element in the specimen times their fractions. The effect of the grid can probably be ignored (unless the illuminated part of the specimen is over a grid bar, which would greatly increase heat conduction). The parameters necessary to do the calculation are the stopping powers, the heat conductivity, and the geometry of the specimen. Then one can set heat in = heat out and solve for the temperature for which the equation holds. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 26 -- From tivol-at-caltech.edu Fri Sep 9 14:31:26 2005 5, 26 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89JVQTA002622 5, 26 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 14:31:26 -0500 5, 26 -- Received: from earth-dog (earth-dog [192.168.1.3]) 5, 26 -- by earth-ox-postvirus (Postfix) with ESMTP id E144F10A6DE 5, 26 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 12:31:25 -0700 (PDT) 5, 26 -- Received: from water-ox ([192.168.1.10]) 5, 26 -- by earth-dog (MailMonitor for SMTP v1.2.2 ) ; 5, 26 -- Fri, 9 Sep 2005 12:31:25 -0700 (PDT) 5, 26 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 5, 26 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 32AC7342CA 5, 26 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 12:31:23 -0700 (PDT) 5, 26 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 26 -- In-Reply-To: {200509091851.j89IpeXx026370-at-ns.microscopy.com} 5, 26 -- References: {200509091851.j89IpeXx026370-at-ns.microscopy.com} 5, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 26 -- Message-Id: {1edd158ef0feb00a134e16912a444d44-at-caltech.edu} 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 26 -- Subject: Re: [Microscopy] temperature of 100 kV beam 5, 26 -- Date: Fri, 9 Sep 2005 12:33:58 -0700 5, 26 -- To: microscopy-at-msa.microscopy.com 5, 26 -- X-Mailer: Apple Mail (2.622) 5, 26 -- X-Spam-Status: No, hits=0.0 tagged_above=-100000.0 required=5.0 5, 26 -- X-Spam-Level: ==============================End of - Headers==============================
On Sep 9, 2005, at 11:51 AM, bozzola-at-siu.edu wrote: } } } A colleague, who is experiencing specimen damage in the TEM, inquired } } if anyone knew the temperature generated on the specimen by the } } electron beam. I realize that there are a lot of variables here, but } } even a range of temperatures would be useful. } Dear John, } At steady state, the heat in, which is the stopping power, dE/dx, in } units of joules/meter times the electron beam current times the } specimen thickness, ...
This gives an upper limit to the heat deposited in the specimen, since not all the energy loss is converted to heat. Some is carried away by bremsstrahlung, secondary electrons, etc.
} Then one can set heat in = heat out and solve for the temperature for } which the equation holds. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 26 -- From tivol-at-caltech.edu Fri Sep 9 15:20:53 2005 6, 26 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89KKr4W011645 6, 26 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 15:20:53 -0500 6, 26 -- Received: from earth-dog (earth-dog [192.168.1.3]) 6, 26 -- by water-ox-postvirus (Postfix) with ESMTP id 1710C3431B 6, 26 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 13:20:53 -0700 (PDT) 6, 26 -- Received: from earth-ox ([192.168.1.9]) 6, 26 -- by earth-dog (MailMonitor for SMTP v1.2.2 ) ; 6, 26 -- Fri, 9 Sep 2005 13:20:52 -0700 (PDT) 6, 26 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 6, 26 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id 1511E109A0B 6, 26 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 13:20:52 -0700 (PDT) 6, 26 -- Mime-Version: 1.0 (Apple Message framework v622) 6, 26 -- In-Reply-To: {200509091851.j89IpeXx026370-at-ns.microscopy.com} 6, 26 -- References: {200509091851.j89IpeXx026370-at-ns.microscopy.com} 6, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 26 -- Message-Id: {1f84d806a1c28c6ce8f021444458a5a4-at-caltech.edu} 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 26 -- Subject: Re: [Microscopy] temperature of 100 kV beam, Addendum 6, 26 -- Date: Fri, 9 Sep 2005 13:23:27 -0700 6, 26 -- To: microscopy-at-msa.microscopy.com 6, 26 -- X-Mailer: Apple Mail (2.622) 6, 26 -- X-Spam-Status: No, hits=0.0 tagged_above=-100000.0 required=5.0 6, 26 -- X-Spam-Level: ==============================End of - Headers==============================
.....If it is a stain related issue, you may want to try embedding in LR White methylmethacrylate resin. This resin is quite different from epoxy resins in that you can use a acid fuchsin/methylene blue stain to produce an H&E like stained section...
I have used (successfully) another "H & E"-like stain with epon. My reference is an application note (303) from LKB: "Stains for Plastic Embedded Tissue Sections II. Staining of sections from different animal, human and plant tissues with a methylene blue-azure II-basic fuchsin stain" (Humphrey) - Maj Andersson (April 1977).
The original reference was: Humphrey, CD and Pittman, FE (1974) "A simple methylen blue-azure II-basic fuchsin stain for epoxy-embedded tissue sections" Stain Technology. 49; 9-14.
It gives beautiful results.
Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
One clarification: there is another
-----Original Message----- X-from: Sue.Tyler-at-noaa.gov [mailto:Sue.Tyler-at-noaa.gov] Sent: Wednesday, September 07, 2005 3:33 PM To: Sherwood, Margaret
Ken Tiekotter wrote:
} Sue, } } I misplaced your reply to me, however, I must ask a couple more questions. } I assume you are processing the 70% half into paraffin and the other half } goes into resin. } } Rather than process all tissues in plastic, I assume the material needs to } be scanned by the pathologist with the light microscope. If he or she finds } areas of interest, you are then required to process the other half for } ultrastructural TEM examination: ok so far? } } Are you specifically embedding separately because of the size of the } amphipods or because you are required to stain with either H&E or other more } specific stains. } } If it is a size related issue, you will more than likely need to process } both halves to paraffin and resin. } } If it is a stain related issue, you may want to try embedding in LR White } methylmethacrylate resin. This resin is quite different from epoxy resins } in that you can use a acid fuchsin/methylene blue stain to produce an H&E } like stained section. } } If you pathologist is not too concerned with leaching of material over time, } you might be better off leaving your specimens in your primary fixative } instead of cacodylate buffer. If you are getting growth in your buffer over } time, you must be stored for several months. Even this length of time will } leach proteins. } } I asked about your use or if you use a digital ccd camera on your TEM } because the ccd will provide incredible contrast. I used to use a } glutaraldehyde/osmium/tannic acid/osmium fixative for contrast and } penetration. With the digital camera, I don't need the extra contrast. } } Regards, } Ken } _______________________________________ } Kenneth L. Tiekotter, Adjunct Professor } The University of Portland } Department of Biology } 5000 N Willamette Blvd. } Portland, OR 97203 USA } } Tel.: 503.943.8861 } Email: tiekotte-at-up.edu } } } On 9/7/05 11:17 AM, "Sue.Tyler-at-noaa.gov" {Sue.Tyler-at-noaa.gov} wrote: } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 2, 19 -- From sue.tyler-at-noaa.gov Wed Sep 7 14:28:10 2005 2, 19 -- Received: from hermes.nos.noaa.gov (hermes.nos.noaa.gov [140.90.119.204]) 2, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j87JSARP031904 2, 19 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Sep 2005 14:28:10 -0500 2, 19 -- Received: from [10.60.12.125] ([10.60.12.125]) by 2, 19 -- hermes.nos.noaa.gov (Netscape Messaging Server 4.15) with ESMTP 2, 19 -- id IMGOQY00.RL5; Wed, 7 Sep 2005 15:28:10 -0400 2, 19 -- Message-ID: {431F3F49.2020200-at-noaa.gov} 2, 19 -- Date: Wed, 07 Sep 2005 15:28:09 -0400 2, 19 -- From: "Sue Tyler" {Sue.Tyler-at-noaa.gov} 2, 19 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 2, 19 -- X-Accept-Language: en-us, en 2, 19 -- MIME-Version: 1.0 2, 19 -- To: tiekotte-at-up.edu, microscopy-at-msa.microscopy.com 2, 19 -- Subject: Re: [Microscopy] Clarification of Marine TEM fixative 2, 19 -- References: {BF448709.2977%tiekotte-at-up.edu} 2, 19 -- In-Reply-To: {BF448709.2977%tiekotte-at-up.edu} 2, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 17, 24 -- From MSHERWOOD-at-PARTNERS.ORG Fri Sep 9 15:33:37 2005 17, 24 -- Received: from PHSXCON3.partners.org (phsxcon3.mgh.harvard.edu [132.183.130.41]) 17, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89KXagt020262 17, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Sep 2005 15:33:37 -0500 17, 24 -- Received: from PHSXMB1.partners.org ([132.183.118.117]) by PHSXCON3.partners.org with Microsoft SMTPSVC(6.0.3790.211); 17, 24 -- Fri, 9 Sep 2005 16:33:36 -0400 17, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 17, 24 -- Content-class: urn:content-classes:message 17, 24 -- MIME-Version: 1.0 17, 24 -- Content-Type: text/plain; 17, 24 -- charset="iso-8859-1" 17, 24 -- Subject: RE: [Microscopy] Re: Clarification of Marine TEM fixative 17, 24 -- Date: Fri, 9 Sep 2005 16:33:36 -0400 17, 24 -- Message-ID: {1AF23D0AD12E7444A5DB083CA978B73403F9266D-at-PHSXMB1.partners.org} 17, 24 -- X-MS-Has-Attach: 17, 24 -- X-MS-TNEF-Correlator: 17, 24 -- Thread-Topic: [Microscopy] Re: Clarification of Marine TEM fixative 17, 24 -- Thread-Index: AcWz4vEXXfFpnnDxQJGoxSt1xOdvmwABof9Q 17, 24 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 17, 24 -- To: {Sue.Tyler-at-noaa.gov} 17, 24 -- Cc: {tiekotte-at-up.edu} , {microscopy-at-microscopy.com} 17, 24 -- X-OriginalArrivalTime: 09 Sep 2005 20:33:36.0335 (UTC) FILETIME=[C0FE2DF0:01C5B57D] 17, 24 -- Content-Transfer-Encoding: 8bit 17, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j89KXagt020262 ==============================End of - Headers==============================
Phay Fang Gan wrote: =============================================== Question: May I know the shelf life of the conductive silver paint and carbon paint ? =============================================== This seemingly simple question has a complicated answer.
First, despite "conventional wisdom", the silver paint used in EM labs is not "all the same". In addition to the obvious difference in silver solids between products, and variations in silver colloid size, some paints (including the SPI Supplies brands of silver paints) contain a small amount of an "amyloid" polymer, not enough to affect negatively its conductivity, but enough to greatly enhance its adhesive characteristics.
But this is not the only function of the presence of the amyloid polymer: If one should forget to screw on the cap to their silver paint bottle, the addition of the recommended thinner and a few minutes in a laboratory ultrasonic shaker will quickly "rejuvenated" it and bring it back to life. But those silver paints without the amyloid polymer or perhaps some other polymer that is not so readily dissolved will either be rejuvenated much more slowly or as we have seen, in some cases, not at all.
So if you are using at least certain silver paints, since the life time of the silver colloid is essentially infinite, and solvent that evaporates can be replaced with the right thinner (even to the point of its having dried out into a brick), there is no real lifetime limit. There are legal and other reasons why manufacturers might publish some "expiration" date for such products but from a practical stand point, at least for some brands of silver paint, the life time is essentially infinite.
But if your question had to do more with the lifetime of the silver paint product unopened, and sitting on the shelf, then this has more to do with the closure system, including the heat seal. Again, not all closure systems are the same. I have seen some silver paint products on the shelf of certain distributors in foreign countries where the paint was as it was delivered ten or more years prior. And I have also seen paints of other brands that had dried out into bricks after only a few years on the shelf.
When discussing the shelf lives of carbon paints, you could almost substitute "carbon" for "silver" above (except that for those carbon paints that do contain a polymer, it is not (to my knowledge an amyloid type). The shelf life of at least some carbon paints is indeed just as infinite as their silver paint counterparts.
Rather than commenting further on where the SPI Supplies brand family of silver and carbon paints fits into this picture, I would refer anyone interested to our silver and carbon paints "main page" at URL www.2spi.com/catalog/spec_prep/cond_paints.html and then draw your own conclusions.
Disclaimer: SPI Supplies is a major supplier worldwide of the SPI Supplies® and Dotite® brands of silver paint and SPI-Chem brand of carbon paint products used in electron microscopy and surface analysis applications.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 15, 23 -- From cgarber-at-2spi.com Fri Sep 9 17:04:28 2005 15, 23 -- Received: from mailrelay.eurospot.com (mailrelay.eurospot.com [62.39.81.196]) 15, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89M4ScW029667 15, 23 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 17:04:28 -0500 15, 23 -- Received: from ibm1x23g2abfyg (85-18-163-242.ip.fastwebnet.it [85.18.163.242]) 15, 23 -- by mailrelay.eurospot.com (Postfix) with SMTP id 4719B286EA8 15, 23 -- for {microscopy-at-msa.microscopy.com} ; Sat, 10 Sep 2005 00:04:27 +0200 (CEST) 15, 23 -- Message-ID: {009901c5b58a$6f7c5260$8130a8c0-at-ibm1x23g2abfyg} 15, 23 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 15, 23 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 15, 23 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 15, 23 -- Subject: Shelf life of silver paint 15, 23 -- Date: Fri, 9 Sep 2005 12:47:03 -0400 15, 23 -- MIME-Version: 1.0 15, 23 -- Content-Type: text/plain; 15, 23 -- format=flowed; 15, 23 -- charset="Windows-1252"; 15, 23 -- reply-type=original 15, 23 -- Content-Transfer-Encoding: 8bit 15, 23 -- X-Priority: 3 15, 23 -- X-MSMail-Priority: Normal 15, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 15, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Liberty Science Center in Liberty State Park, Jersey City, NJ, has a lightly used 1982 Zeiss 940A SEM available immediately. This scope was serviced yearly until 2000 and used little after that up to about 6 months ago. Both vacuum pumps and chiller are working but the SEM needs some work to get operational again.
Anyone interested in this microscope, please contact Betty Faber, bfaber-at-lsc.org.
Betty Faber, Ph.D. Leader Program Development Learning and Teaching Liberty Science Center
==============================Original Headers============================== 7, 17 -- From BFABER-at-lsc.org Fri Sep 9 18:12:15 2005 7, 17 -- Received: from las.lsc.org (firewall.lsc.org [209.101.152.6]) 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89NCFXT006229 7, 17 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Sep 2005 18:12:15 -0500 7, 17 -- Received: from domain-MTA by las.lsc.org 7, 17 -- with Novell_GroupWise; Fri, 09 Sep 2005 19:12:14 -0400 7, 17 -- Message-Id: {s321de8e.000-at-las.lsc.org} 7, 17 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 7, 17 -- Date: Fri, 09 Sep 2005 19:11:44 -0400 7, 17 -- From: "BETTY FABER" {BFABER-at-lsc.org} 7, 17 -- To: {Microscopy-at-MSA.Microscopy.Com} 7, 17 -- Subject: SEM for sale 7, 17 -- Mime-Version: 1.0 7, 17 -- Content-Type: text/plain; charset=US-ASCII 7, 17 -- Content-Disposition: inline 7, 17 -- Content-Transfer-Encoding: 8bit 7, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j89NCFXT006229 ==============================End of - Headers==============================
I can comment on the carbon paint. I use a bottle until it's totally gone and I can't get any more carbon in solution using isopropanol. I should say I use SPI's conductive carbon paint because it cleans up easily and can use isopropanol as the diluent (even though Dr. Garber would recommend I use their thinner instead). I've been using my current bottle for about 5 years now for SEM and FIB work. When it gets to the consistency of chocolate pudding, I add a couple of mls of alcohol and shake for around 5-10 minutes. As others have recommended, definitely keep the lid on tight and shake well before and after use.
pgan-at-ap.ansell.com wrote:
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-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 5, 23 -- From r-holdford-at-ti.com Fri Sep 9 18:15:01 2005 5, 23 -- Received: from go4.ext.ti.com (go4.ext.ti.com [192.91.75.132]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89NF104010125 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Sep 2005 18:15:01 -0500 5, 23 -- Received: from dlep30.itg.ti.com ([157.170.139.157]) 5, 23 -- by go4.ext.ti.com (8.13.4/8.13.4) with ESMTP id j89NEf7C011862; 5, 23 -- Fri, 9 Sep 2005 18:14:46 -0500 (CDT) 5, 23 -- Received: from [156.117.194.45] (localhost [127.0.0.1]) 5, 23 -- by dlep30.itg.ti.com (8.12.11/8.12.11) with ESMTP id j89NEenV010423; 5, 23 -- Fri, 9 Sep 2005 18:14:41 -0500 (CDT) 5, 23 -- Message-ID: {4322175E.9050706-at-ti.com} 5, 23 -- Date: Fri, 09 Sep 2005 18:14:38 -0500 5, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 5, 23 -- Organization: SC Packaging Development -- FA Development 5, 23 -- User-Agent: Mozilla Thunderbird 0.8 (Windows/20040913) 5, 23 -- X-Accept-Language: en-us, en 5, 23 -- MIME-Version: 1.0 5, 23 -- To: pgan-at-ap.ansell.com, MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 23 -- Subject: Re: [Microscopy] viaWWW: shelf life of the conductive paint 5, 23 -- References: {200509090754.j897sdJR010142-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {200509090754.j897sdJR010142-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The temperature in the sample due to the energy being deposited in it is very dependent on the thickness of the sample. At 120 keV, if I did not deposit a sufficient layer of carbon on glass cross sections, the glass would soften under the beam. 100 keV would be worse. When I used a 200 keV machine, the problem essentially went away. For 100 keV, to avoid the problem, the illuminated area must be very thin.
One of the other things that I did that seemed to help with glass samples was to use a piece of Si as the mate to the cross section in the stack. The Si seems to take more of the heat away from the sample. Either that or it supplied a temperature insensitive portion of the total sample to prevent the sagging.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-------- Original Message -------- } From: bozzola-at-siu.edu } Sent: Friday, September 09, 2005 2:54 PM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] temperature of 100 kV beam } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } A colleague, who is experiencing specimen damage in the TEM, inquired } if anyone knew the temperature generated on the specimen by the } electron beam. I realize that there are a lot of variables here, but } even a range of temperatures would be useful. } } Here are his operating parameters: } } } kV = 100 } spot size = 2 micrometer (specimen was examined at approximately 2 } micrometer spot size) } specimen = ceramic containing metal particles (Ti) } magnification = 100K to 600K } electron gun = LaB6 } vacuum = turbo pumped to 10-5 Pa } substrate that specimen was mounted on = Formvar/carbon coated grid } } } -- } ############################################################## } John J. Bozzola, Ph.D., Director } I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) } 750 Communications Drive - MC 4402 } Southern Illinois University } Carbondale, IL 62901 U.S.A. } Phone: 618-453-3730 } Email: bozzola-at-siu.edu } ############################################################## } } ==============================Original Headers============================== } 6, 16 -- From bozzola-at-siu.edu Fri Sep 9 13:51:34 2005 } 6, 16 -- Received: from abbmta1.siu.edu (abbmta1.siu.edu [131.230.254.205]) } 6, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89IpYTY026191 } 6, 16 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 13:51:34 -0500 } 6, 16 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) } 6, 16 -- by abbmta1.siu.edu (Switch-3.1.0/Switch-3.1.0) with ESMTP id j89IpWlh021857 } 6, 16 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 13:51:34 -0500 (CDT) } 6, 16 -- Mime-Version: 1.0 } 6, 16 -- X-Sender: bozzola-at-saluki-mail.siu.edu } 6, 16 -- Message-Id: {p06110432bf4789710b4a-at-[131.230.177.142]} } 6, 16 -- Date: Fri, 9 Sep 2005 13:51:43 -0500 } 6, 16 -- To: Microscopy-at-msa.microscopy.com } 6, 16 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } 6, 16 -- Subject: temperature of 100 kV beam } 6, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 6, 16 -- X-MASF: 0.00% } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 23 -- From walck-at-southbaytech.com Fri Sep 9 23:21:48 2005 5, 23 -- Received: from smtp06.safesecureweb.com (smtp06.safesecureweb.com [66.241.193.136]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8A4LlAc025659 5, 23 -- for {microscopy-at-microscopy.com} ; Fri, 9 Sep 2005 23:21:47 -0500 5, 23 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 5, 23 -- by smtp06.safesecureweb.com (Postfix) with ESMTP id 88C2A554246; 5, 23 -- Sat, 10 Sep 2005 00:21:45 -0400 (EDT) 5, 23 -- Received: from mail15.safesecureweb.com (unknown [192.168.2.180]) 5, 23 -- by smtp06.safesecureweb.com (Postfix) with ESMTP id 61575554059; 5, 23 -- Sat, 10 Sep 2005 00:21:43 -0400 (EDT) 5, 23 -- MIME-Version: 1.0 5, 23 -- Date: Sat, 10 Sep 2005 00:19:49 -0400 5, 23 -- Content-Type: text/plain; 5, 23 -- charset=iso-8859-1 5, 23 -- Subject: re: [Microscopy] temperature of 100 kV beam 5, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 5, 23 -- Reply-To: Walck-at-southbaytech.com 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- Cc: {bozzola-at-siu.edu} 5, 23 -- Message-ID: {ac7173abee2147a4917de9a1244801ca-at-southbaytech.com} 5, 23 -- X-Virus-Scanned: by amavisd-new-20030616-p10 at safesecureweb.com 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8A4LlAc025659 ==============================End of - Headers==============================
I don't have the answer to this question but when I was renovating my TEM I was playing around with a sample of actinolite asbestos. When we increased the power of the beam we easily melted the fibres. Theese were thick fibres, don't think any of them were electron transparent so the maximum amount of energy was absorbed by the specimen. If my memory doesn't fail me we used 100kV and no apertures. As the TEM wasn't fully operational I have no idea of the size of the fibres.
Göran, electron microscopist wannabe
bozzola-at-siu.edu wrote:
} A colleague, who is experiencing specimen damage in the TEM, inquired } if anyone knew the temperature generated on the specimen by the } electron beam. I realize that there are a lot of variables here, but } even a range of temperatures would be useful. } } Here are his operating parameters: } } } kV = 100 } spot size = 2 micrometer (specimen was examined at approximately 2 } micrometer spot size) } specimen = ceramic containing metal particles (Ti) } magnification = 100K to 600K } electron gun = LaB6 } vacuum = turbo pumped to 10-5 Pa } substrate that specimen was mounted on = Formvar/carbon coated grid } } } }
==============================Original Headers============================== 5, 20 -- From axelsson-at-acc.umu.se Sat Sep 10 08:23:58 2005 5, 20 -- Received: from spider.neab.net (1-1-10-48a.um.um.bostream.se [82.182.239.55]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8ADNvCS006815 5, 20 -- for {Microscopy-at-msa.microscopy.com} ; Sat, 10 Sep 2005 08:23:58 -0500 5, 20 -- Received: from [192.168.1.3] (eddie.neab.net [192.168.1.3]) 5, 20 -- by spider.neab.net (8.9.3p2/8.9.3/Debian 8.9.3-21) with ESMTP id PAA26361 5, 20 -- for {Microscopy-at-msa.microscopy.com} ; Sat, 10 Sep 2005 15:52:49 +0200 5, 20 -- X-Authentication-Warning: spider.neab.net: Host eddie.neab.net [192.168.1.3] claimed to be [192.168.1.3] 5, 20 -- Message-ID: {4322DF21.9060402-at-acc.umu.se} 5, 20 -- Date: Sat, 10 Sep 2005 15:26:57 +0200 5, 20 -- From: =?ISO-8859-1?Q?G=F6ran_Axelsson?= {axelsson-at-acc.umu.se} 5, 20 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 5, 20 -- X-Accept-Language: en-us, en 5, 20 -- MIME-Version: 1.0 5, 20 -- To: Microscopy-at-msa.microscopy.com 5, 20 -- Subject: Re: [Microscopy] temperature of 100 kV beam 5, 20 -- References: {200509091851.j89Ipca0026280-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200509091851.j89Ipca0026280-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (brandon-at-earthlab.net) from http://microscopy.org/MicroscopyListserver/MLFormMail.html on Sunday, September 11, 2005 at 12:32:37 ---------------------------------------------------------------------------
Email: brandon-at-earthlab.net Name: Brandon Keim
Organization: Columbia University Graduate School of Journalism
Title-Subject: [Filtered] MListserver: Electron Microscopy Article Questions
Question: Dear All,
I am a freelance journalist and graduate student at the Columbia Journalism School, with a concentration in science and health writing. For a class assignment I am writing about the present state and history of electron microscopy.
If possible, I'd like to talk briefly with some of you about what electron microscopy has made possible, how it has evolved and will continue to evolve, and what you consider important to know. If anyone is interested, please feel free to get in touch; my deadline, however, is Tuesday, so the next day or two would be best.
Sincerely,
Brandon Keim
Freelance Writer Columbia School of Journalism c: 617 233 5346 e: brandon-at-earthlab.net
I would like to post the following Career Opportunity:
SENIOR RESEARCH CHEMIST (Microscopy / X-ray)
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For more information regarding Arkema, please visit our website: www.arkemagroup.com.
--------------------------------------------------------------------------------------------------------- Nikola M. Juhasz, Ph.D. Manager, Systems and Materials Analysis Analytical and Systems Research Arkema Inc. 900 First Avenue King of Prussia, PA 19406 (610) 878-6408 (610) 878-6196 fax nikola.juhasz-at-arkemagroup.com ---------------------------------------------------------------------------------------------------------
==============================Original Headers============================== 15, 13 -- From zaluzec-at-microscopy.com Sun Sep 11 13:49:51 2005 15, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 15, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8BInoHJ007114 15, 13 -- for {microscopy-at-microscopy.com} ; Sun, 11 Sep 2005 13:49:50 -0500 15, 13 -- Mime-Version: 1.0 15, 13 -- X-Sender: (Unverified) 15, 13 -- Message-Id: {p06110402bf4a2ca4a120-at-[206.69.208.22]} 15, 13 -- Date: Sun, 11 Sep 2005 13:49:49 -0500 15, 13 -- To: microscopy-at-microscopy.com 15, 13 -- From: Nikola JUHASZ {nikola.juhasz-at-arkemagroup.com} (by way of 15, 13 -- MicroscopyListserver) 15, 13 -- Subject: viaWWW: Career Opportunity: 15, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I think Bill Tivol's outline of specimen heating is fine, and a very worthwhile exercise. One of the consequences of the T^4 power for radiated heat is that you don't get much heat loss by radiation below about 200C (lots of hand waving and caveats here, this is a very rough number). However I'd like to add to the emphasis on the importance of a good heat sink. I know from experience that I can fry a lift-out FIB section of InP on a holey carbon grid in a 120 kV TEM (melting point 1060C, but starts to decompose around 550C). Not very enjoyable if you just spent a few hundred £ getting the damn thing made. On the other hand I never have any problems with conventionally ion milled specimens, which have 20um thick InP on a Cu grid on the outside, tapering to the hole in the middle, and even materials like PbSn solders (melting point 183C) and Au/Ge multilayers (interdiffusion {100C) are fine if there is a good thermal path to the support grid. From your description of the sample I guess it's a lift-out FIB section. As others have pointed out, higher kV will help since the beam-specimen interaction is less. Or you'll have no problems with a H-bar section, which has a massive heat sink all around the thin area (but you won't be able to do meaningful X-ray analysis). Or you may have to go back to the old ways of making specimens..
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: 09 September 2005 19:53 To: Richard Beanland
A colleague, who is experiencing specimen damage in the TEM, inquired if anyone knew the temperature generated on the specimen by the electron beam. I realize that there are a lot of variables here, but even a range of temperatures would be useful.
Here are his operating parameters:
kV = 100 spot size = 2 micrometer (specimen was examined at approximately 2 micrometer spot size) specimen = ceramic containing metal particles (Ti) magnification = 100K to 600K electron gun = LaB6 vacuum = turbo pumped to 10-5 Pa substrate that specimen was mounted on = Formvar/carbon coated grid
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu ##############################################################
==============================Original Headers============================== 6, 16 -- From bozzola-at-siu.edu Fri Sep 9 13:51:34 2005 6, 16 -- Received: from abbmta1.siu.edu (abbmta1.siu.edu [131.230.254.205]) 6, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j89IpYTY026191 6, 16 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 13:51:34 -0500 6, 16 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 6, 16 -- by abbmta1.siu.edu (Switch-3.1.0/Switch-3.1.0) with ESMTP id j89IpWlh021857 6, 16 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 9 Sep 2005 13:51:34 -0500 (CDT) 6, 16 -- Mime-Version: 1.0 6, 16 -- X-Sender: bozzola-at-saluki-mail.siu.edu 6, 16 -- Message-Id: {p06110432bf4789710b4a-at-[131.230.177.142]} 6, 16 -- Date: Fri, 9 Sep 2005 13:51:43 -0500 6, 16 -- To: Microscopy-at-msa.microscopy.com 6, 16 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 6, 16 -- Subject: temperature of 100 kV beam 6, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 16 -- X-MASF: 0.00% ==============================End of - Headers==============================
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==============================Original Headers============================== 19, 29 -- From richard.beanland-at-bookham.com Mon Sep 12 03:29:08 2005 19, 29 -- Received: from mail83.messagelabs.com (mail83.messagelabs.com [195.245.231.83]) 19, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j8C8T7RN028888 19, 29 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 03:29:07 -0500 19, 29 -- X-VirusChecked: Checked 19, 29 -- X-Env-Sender: richard.beanland-at-bookham.com 19, 29 -- X-Msg-Ref: server-11.tower-83.messagelabs.com!1126513746!25341056!1 19, 29 -- X-StarScan-Version: 5.4.15; banners=bookham.com,-,- 19, 29 -- X-Originating-IP: [213.249.209.179] 19, 29 -- Received: (qmail 32423 invoked from network); 12 Sep 2005 08:29:06 -0000 19, 29 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 19, 29 -- by server-11.tower-83.messagelabs.com with SMTP; 12 Sep 2005 08:29:06 -0000 19, 29 -- Content-class: urn:content-classes:message 19, 29 -- MIME-Version: 1.0 19, 29 -- Content-Type: text/plain; 19, 29 -- charset="iso-8859-1" 19, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 19, 29 -- Subject: RE: [Microscopy] temperature of 100 kV beam 19, 29 -- Date: Mon, 12 Sep 2005 09:29:05 +0100 19, 29 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBC93-at-cas-smx-01.caswell1.europe.bkhm.net} 19, 29 -- X-MS-Has-Attach: 19, 29 -- X-MS-TNEF-Correlator: 19, 29 -- Thread-Topic: [Microscopy] temperature of 100 kV beam 19, 29 -- Thread-Index: AcW1b7AVgtlQVL67Spm8UMtfud5/9QCAZmAA 19, 29 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 19, 29 -- To: {bozzola-at-siu.edu} 19, 29 -- Cc: {microscopy-at-microscopy.com} 19, 29 -- Content-Transfer-Encoding: 8bit 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8C8T7RN028888 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pcosta33-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 12, 2005 at 03:30:26 ---------------------------------------------------------------------------
Question: I have recently carried out some EDX analysis on a Ga-Au alloy for which I would like to a have rough idea of composition. The analysis was made for an object which is about 40 nm thick with a STEM probe in a FEG 200 kV instrument. Since the Au K signals come at too high energy for our detector, my question is if it is possible to compare the K-shell band of Ga with the L-shell band of Au. Or would it be more correct to do that analysis comparing the two L-shell bands of Ga and Au?
Since all the Au-Ga compounds are known, all you need is a rough value of composition to say which one it is. I usually take a diffraction pattern or two and compare measured d-values with the international crystallographic database if there's any uncertainty. You may have to do low angle convergent beam (using a tiny condenser aperture) rather than selected area diffraction if the grains are small in a multi-phase compound. The nice thing about TEM is that you can get EDX and diffraction analysis from the same grain. As for the EDX analysis, using different lines (K,L,M..) shouldn't be a problem anyway - if you had to do a proper job, you would be comparing it with a known standard and you can use whichever lines you like as long as there's no strong overlaps (I'm happy to be corrected on this by people who do this every day, I'm no expert)..
-----Original Message----- ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pcosta33-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 12, 2005 at 03:30:26 ---------------------------------------------------------------------------
Question: I have recently carried out some EDX analysis on a Ga-Au alloy for which I would like to a have rough idea of composition. The analysis was made for an object which is about 40 nm thick with a STEM probe in a FEG 200 kV instrument. Since the Au K signals come at too high energy for our detector, my question is if it is possible to compare the K-shell band of Ga with the L-shell band of Au. Or would it be more correct to do that analysis comparing the two L-shell bands of Ga and Au?
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you should use Ga-Ka and Au-La because of the comparable excitation and absorption conditions with energy of 9..10 keV. This is the best choice, even if your detector would be able to detect Au-K. But take into mind for (only rough) concentration determinations, if the Ga/Au- concentration ratio is expected with 1/1, then the peak-heights or pak-net counts are like about 6/10.
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==============================Original Headers============================== 8, 19 -- From eggert-at-mikroanalytik.de Mon Sep 12 08:59:00 2005 8, 19 -- Received: from Mail1.KONTENT.De (mail1.kontent.de [81.88.34.36]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8CDx0wE025764 8, 19 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 08:59:00 -0500 8, 19 -- Received: from mikroanalytik.de (p54BDE49C.dip.t-dialin.net [84.189.228.156]) 8, 19 -- by Mail1.KONTENT.De (Postfix) with ESMTP id 47A711043878; 8, 19 -- Mon, 12 Sep 2005 15:59:05 +0200 (CEST) 8, 19 -- Message-ID: {43258B1F.8000601-at-mikroanalytik.de} 8, 19 -- Date: Mon, 12 Sep 2005 16:05:19 +0200 8, 19 -- From: Frank Eggert {eggert-at-mikroanalytik.de} 8, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; de-DE; rv:1.4) Gecko/20030619 Netscape/7.1 (ax) 8, 19 -- X-Accept-Language: de, de-de, en 8, 19 -- MIME-Version: 1.0 8, 19 -- To: pcosta33-at-hotmail.com, microscopy-at-microscopy.com 8, 19 -- Subject: Re: [Microscopy] viaWWW: EDX analysis 8, 19 -- References: {200509121246.j8CCkBlW011680-at-ns.microscopy.com} 8, 19 -- In-Reply-To: {200509121246.j8CCkBlW011680-at-ns.microscopy.com} 8, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I really like the DVD idea...I don't do as much microscopy as I used to and I'm getting rusty! Or maybe I have that disease, you all know the one I mean, CRS...Can't Remember Stuff! A little help is always welcome and DVDs would be good 'cause you could pull them out when you had a specific question or problem. I think this is a great idea.
Dorrance
-----Original Message----- X-from: gwe-at-ufl.edu [mailto:gwe-at-ufl.edu] Sent: Thursday, September 08, 2005 12:05 PM To: McLean, Dorrance
Dear MSA members and other listers, As wrangler of the MSA video collection I am often asked if we have a full basic course in electron microscopy available on DVD. We do not. I can also infer from folks who purchase 20 or 30 or 40 or 50 DVDs that they intend to teach themselves what they need to know from our recorded tutorials. (The record purchase is 52 DVDs that was filled last month.). In light of the fact that EM coures are disappearing at universities around the country, the Education Committee of MSA is considering the idea of putting together a basic course or courses on electron microscopy and make them available on DVD. Something that would present the technology in an organized fashion and be comprehensive enough to put a student in a position to begin work in the area. If such a project is undertaken, Steve Barlow and Howard Berg have agreed to work with me on coordinating it. First, we would like to hear from the community on whether or not they think this is a good idea. Second we would be looking for volunteers who might be willing to record a lesson with supporting demonstrations and other visual representations that could flesh out the coverage. We don't want just a talking head. We would also be looking for folks who would be willing to share the syllabi from their courses so that we might determine how to go about organizing such a thing. Any and all feedback is welcome.
Regarsd to all, Greg
-- Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, Electron Microscopy P.O. Box 118525 217 Carr Hall University of Florida Gainesville, FL 32611 Phone: 352-392-1295 Fax: 352 846 0251
==============================Original Headers============================== 7, 21 -- From gwe-at-ufl.edu Thu Sep 8 14:02:51 2005 7, 21 -- Received: from smtp.ufl.edu (sp42en1.nerdc.ufl.edu [128.227.74.42]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j88J2o5c032635 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 14:02:51 -0500 7, 21 -- Received: from [127.0.0.1] (pc2524c.dhcp.clas.ufl.edu [128.227.60.197]) 7, 21 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j88J2lEF127796 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2005 15:02:49 -0400 7, 21 -- Message-ID: {43208ADE.6050104-at-ufl.edu} 7, 21 -- Date: Thu, 08 Sep 2005 15:02:54 -0400 7, 21 -- From: Greg Erdos {gwe-at-ufl.edu} 7, 21 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 7, 21 -- X-Accept-Language: en-us, en 7, 21 -- MIME-Version: 1.0 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- Subject: Tutorials 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 7, 21 -- X-UFL-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 7, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 7, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
==============================Original Headers============================== 18, 29 -- From dmclea-at-sandia.gov Mon Sep 12 10:14:49 2005 18, 29 -- Received: from mm02snlnto.sandia.gov (mm02snlnto.sandia.gov [132.175.109.21]) 18, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8CFEmco011213 18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 10:14:49 -0500 18, 29 -- Received: from 132.175.109.1 by MM01SNLNTO.sandia.gov with ESMTP ( 18, 29 -- Tumbleweed MMS SMTP Relay 01 (MMS v5.6.3)); Mon, 12 Sep 2005 09:14:37 18, 29 -- -0600 18, 29 -- X-Server-Uuid: 2C1074A8-2B28-4DE3-9F7D-FF40AE090BA2 18, 29 -- Received: from ES23SNLNT.srn.sandia.gov (ec04snlnt.sandia.gov 18, 29 -- [134.253.164.156] (may be forged)) by mailgate.sandia.gov ( 18, 29 -- 8.13.3/8.13.3) with ESMTP id j8CFEYiu019626 for 18, 29 -- {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 09:14:36 -0600 (MDT) 18, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 18, 29 -- Content-class: urn:content-classes:message 18, 29 -- MIME-Version: 1.0 18, 29 -- Subject: RE: [Microscopy] Tutorials 18, 29 -- Date: Mon, 12 Sep 2005 09:14:35 -0600 18, 29 -- Message-ID: {CA82EF34F71386438BB876223415C48B759D9E-at-ES23SNLNT.srn.sandia.gov} 18, 29 -- X-MS-Has-Attach: 18, 29 -- X-MS-TNEF-Correlator: 18, 29 -- Thread-Topic: [Microscopy] Tutorials 18, 29 -- Thread-Index: AcW0qEBz4HecnAn0TduG37SLo00PgADA011w 18, 29 -- From: "McLean, Dorrance" {dmclea-at-sandia.gov} 18, 29 -- To: microscopy-at-microscopy.com 18, 29 -- X-WSS-ID: 6F3B44D71AO3647587-01-01 18, 29 -- Content-Type: text/plain; 18, 29 -- charset=us-ascii 18, 29 -- Content-Transfer-Encoding: 8bit 18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8CFEmco011213 ==============================End of - Headers==============================
for years i've been able to do the stats on my immunogold quite easily. unfortunately i have a project which requires dealing with odd shaped granules and inclusions in cells, and labeling on the membranes vs not on the membranes. i'm afraid i'm going to have to come into the computer age here, finally.
i would appreciate the advice of the list on simple programs which will allow me to use a stylus to draw around the perimeter of the region in the micrograph which needs to be analysed, and then give me the total area within the region identified. don't need any more analysis. i can still count the black spots - the eyes haven't gone yet.
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 6, 17 -- From paul_hazelton-at-umanitoba.ca Mon Sep 12 15:47:08 2005 6, 17 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 6, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8CKl8Zb023468 6, 17 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 15:47:08 -0500 6, 17 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 6, 17 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id j8CKl6oS001337; 6, 17 -- Mon, 12 Sep 2005 15:47:07 -0500 (CDT) 6, 17 -- Message-ID: {4325E94F.F42E6F41-at-umanitoba.ca} 6, 17 -- Date: Mon, 12 Sep 2005 15:47:11 -0500 6, 17 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 6, 17 -- X-Mailer: Mozilla 4.78 [en] (Win98; U) 6, 17 -- X-Accept-Language: en,pdf 6, 17 -- MIME-Version: 1.0 6, 17 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 6, 17 -- Subject: image analysis 6, 17 -- Content-Type: text/plain; charset=us-ascii 6, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You can do it with Photoshop! just trace the area and look at the histogram for total pixels counted. used Excel to convert pixels to sq. microns. good luck. tom
At 03:48 PM 09/12/05, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
...or you could use one of the available image processing tools to do exactly what you want. Here is a link to our website (http://www.soft-imaging.com/rd/english/433.htm, click on the "gold labelling" line on the right side), but there are other programs out there that do this.
Mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: phillipst-at-missouri.edu [mailto:phillipst-at-missouri.edu] Sent: Monday, September 12, 2005 3:04 PM To: Mike Bode
You can do it with Photoshop! just trace the area and look at the histogram for total pixels counted. used Excel to convert pixels to sq. microns. good luck. tom
At 03:48 PM 09/12/05, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Image J will do it also, including some stats about the area you draw. David
On Sep 12, 2005, at 5:04 PM, phillipst-at-missouri.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } You can do it with Photoshop! just trace the area and look at the } histogram for total pixels counted. used Excel to convert pixels to } sq. } microns. good luck. tom } } At 03:48 PM 09/12/05, you wrote: } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } for years i've been able to do the stats on my immunogold quite } } easily. } } unfortunately i have a project which requires dealing with odd shaped } } granules and inclusions in cells, and labeling on the membranes vs not } } on the membranes. i'm afraid i'm going to have to come into the } } computer age here, finally. } } } } i would appreciate the advice of the list on simple programs which } } will } } allow me to use a stylus to draw around the perimeter of the region in } } the micrograph which needs to be analysed, and then give me the total } } area within the region identified. don't need any more analysis. i } } can } } still count the black spots - the eyes haven't gone yet. } } } } paul } } } } } } } } Paul R. Hazelton, PhD } } Electron Microscope Unit } } University of Manitoba } } Department of Medical Microbiology } } 531 Basic Medical Sciences Building } } 730 William Avenue } } Winnipeg, Manitoba, Canada, R3E 0W3 } } e-mail: paul_hazelton-at-umanitoba.ca } } Phone:204-789-3313 } } Pager:204-931-9354 } } Cell:204-781-1502 } } Fax:204-789-3926 } } } } ==============================Original } } Headers============================== } } 6, 17 -- From paul_hazelton-at-umanitoba.ca Mon Sep 12 15:47:08 2005 } } 6, 17 -- Received: from electra.cc.umanitoba.ca } } (electra.cc.umanitoba.ca } } [130.179.16.23]) } } 6, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } j8CKl8Zb023468 } } 6, 17 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 } } 15:47:08 } } -0500 } } 6, 17 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca } } [140.193.11.160]) } } 6, 17 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP } } id } } j8CKl6oS001337; } } 6, 17 -- Mon, 12 Sep 2005 15:47:07 -0500 (CDT) } } 6, 17 -- Message-ID: {4325E94F.F42E6F41-at-umanitoba.ca} } } 6, 17 -- Date: Mon, 12 Sep 2005 15:47:11 -0500 } } 6, 17 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} } } 6, 17 -- X-Mailer: Mozilla 4.78 [en] (Win98; U) } } 6, 17 -- X-Accept-Language: en,pdf } } 6, 17 -- MIME-Version: 1.0 } } 6, 17 -- To: Microscopy Listserver {microscopy-at-microscopy.com} } } 6, 17 -- Subject: image analysis } } 6, 17 -- Content-Type: text/plain; charset=us-ascii } } 6, 17 -- Content-Transfer-Encoding: 7bit } } ==============================End of - } } Headers============================== } } Thomas E. Phillips, PhD } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } } 573-882-4712 (office) } 573-882-0123 (fax) } PhillipsT-at-missouri.edu } } } } ==============================Original } Headers============================== } 9, 21 -- From PhillipsT-at-missouri.edu Mon Sep 12 16:01:57 2005 } 9, 21 -- Received: from um-exproto8.um.umsystem.edu } (um-exproto8.um.umsystem.edu [207.160.151.48]) } 9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j8CL1vV4031983 } 9, 21 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 12 Sep 2005 } 16:01:57 -0500 } 9, 21 -- Received: from um-exproto8.um.umsystem.edu } ([207.160.151.148]) by um-exproto8.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.1830); } 9, 21 -- Mon, 12 Sep 2005 16:01:56 -0500 } 9, 21 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) } by um-exproto8.um.umsystem.edu over TLS secured channel with Microsoft } SMTPSVC(6.0.3790.1830); } 9, 21 -- Mon, 12 Sep 2005 16:01:55 -0500 } 9, 21 -- Message-Id: } {6.0.0.22.2.20050912160219.01f5e920-at-pop.missouri.edu} } 9, 21 -- X-Sender: phillipst-at-pop.missouri.edu } 9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22 } 9, 21 -- Date: Mon, 12 Sep 2005 16:03:56 -0500 } 9, 21 -- To: paul_hazelton-at-umanitoba.ca } 9, 21 -- From: Tom Phillips {phillipst-at-missouri.edu} } 9, 21 -- Subject: Re: [Microscopy] image analysis } 9, 21 -- Cc: Microscopy-at-msa.microscopy.com } 9, 21 -- In-Reply-To: {200509122048.j8CKmx6t025112-at-ns.microscopy.com} } 9, 21 -- References: {200509122048.j8CKmx6t025112-at-ns.microscopy.com} } 9, 21 -- Mime-Version: 1.0 } 9, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 9, 21 -- X-OriginalArrivalTime: 12 Sep 2005 21:01:55.0958 (UTC) } FILETIME=[35495160:01C5B7DD] } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 18 -- From Elliott-at-Arizona.edu Mon Sep 12 17:43:34 2005 5, 18 -- Received: from adios.mbl.edu (adios.MBL.EDU [128.128.172.10]) 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8CMhYNh017462 5, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 12 Sep 2005 17:43:34 -0500 5, 18 -- Received: from [128.128.169.155] (unknown [128.128.169.155]) 5, 18 -- by adios.mbl.edu (Postfix) with ESMTP id D06618B240 5, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 12 Sep 2005 18:45:14 -0400 (EDT) 5, 18 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 18 -- In-Reply-To: {200509122104.j8CL4aZs005528-at-ns.microscopy.com} 5, 18 -- References: {200509122104.j8CL4aZs005528-at-ns.microscopy.com} 5, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 18 -- Message-Id: {69bd1debf4e7864ffc7a50a640c6694b-at-Arizona.edu} 5, 18 -- Content-Transfer-Encoding: 7bit 5, 18 -- From: David Elliott {Elliott-at-Arizona.edu} 5, 18 -- Subject: Re: [Microscopy] Re: image analysis 5, 18 -- Date: Mon, 12 Sep 2005 18:42:51 -0400 5, 18 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 18 -- X-Mailer: Apple Mail (2.622) ==============================End of - Headers==============================
One of our TEM service reps told me that he's been told that many people who looks at polymers in the TEM "pre-burn" their samples under a UV lamp before putting the grids in the column - is this true? If so, how is it done (for how long, distance from bulb, etc.)?
We have a weird "thing" with our TEM that burns a pattern into resin sections and we were discussing ways to pre-burn; chemical stetching doesn't help, and doing it in the column is too slow when there are a lot of samples. If I could do a mass burn I'd be set!
Thanks!
Tamara
|--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward-at-unm.edu |--------------------------------------------------|
==============================Original Headers============================== 5, 14 -- From thoward-at-unm.edu Mon Sep 12 17:50:16 2005 5, 14 -- Received: from unm.edu (f5vs2.unm.edu [64.106.76.41]) 5, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8CMoGFU025970 5, 14 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 17:50:16 -0500 5, 14 -- Received: from [129.24.9.43] (HELO deneb.unm.edu) 5, 14 -- by phact.unm.edu (CommuniGate Pro SMTP 4.3.6) 5, 14 -- with ESMTPS id 27235650 for microscopy-at-microscopy.com; Mon, 12 Sep 2005 16:50:14 -0600 5, 14 -- Date: Mon, 12 Sep 2005 16:50:14 -0600 (MDT) 5, 14 -- From: Tamara Howard {thoward-at-unm.edu} 5, 14 -- To: Microscopy Server {microscopy-at-microscopy.com} 5, 14 -- Subject: help from a polymer TEM person? 5, 14 -- Message-ID: {Pine.LNX.4.62.0509121642550.11474-at-deneb.unm.edu} 5, 14 -- MIME-Version: 1.0 5, 14 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
In a message dated 9/12/05 4:48:36 PM, paul_hazelton-at-umanitoba.ca writes:
} i would appreciate the advice of the list on simple programs which will } allow me to use a stylus to draw around the perimeter of the region in } the micrograph which needs to be analysed, and then give me the total } area within the region identified. don't need any more analysis. i can } still count the black spots - the eyes haven't gone yet.
Just about any program out there - including NIH Image which is free - will do that. But why in the world would you NOT want the program to do the counting, too? Lots of tests have demonstrated that people don't really count things very well. And even if you CAN do it accurately, you certainly can't do it as quickly as the computer.
==============================Original Headers============================== 5, 17 -- From DrJohnRuss-at-aol.com Mon Sep 12 18:16:23 2005 5, 17 -- Received: from imo-d22.mx.aol.com (imo-d22.mx.aol.com [205.188.144.208]) 5, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8CNGN83002167 5, 17 -- for {microscopy-at-ns.microscopy.com} ; Mon, 12 Sep 2005 18:16:23 -0500 5, 17 -- Received: from DrJohnRuss-at-aol.com 5, 17 -- by imo-d22.mx.aol.com (mail_out_v38_r5.3.) id l.190.4806b527 (17377); 5, 17 -- Mon, 12 Sep 2005 19:16:07 -0400 (EDT) 5, 17 -- From: DrJohnRuss-at-aol.com 5, 17 -- Message-ID: {190.4806b527.30576637-at-aol.com} 5, 17 -- Date: Mon, 12 Sep 2005 19:16:07 EDT 5, 17 -- Subject: Re: [Microscopy] image analysis 5, 17 -- To: paul_hazelton-at-umanitoba.ca, microscopy-at-ns.microscopy.com 5, 17 -- MIME-Version: 1.0 5, 17 -- Content-Type: text/plain; charset="US-ASCII" 5, 17 -- Content-Transfer-Encoding: 7bit 5, 17 -- X-Mailer: AOL 5.0 for Mac sub 28 5, 17 -- X-Spam-Flag: NO ==============================End of - Headers==============================
I have ImagePro and MetaMorph and the ImageTool Kit and have used them all successfully. But I disagree with John that using a computer is always easier and better. Maybe if I had written the book on image processing like John Russ, I could take my TEM digital images and have the computer automatically threshold, detect and count the colloidal gold particles against a typical cell background in a reasonable amount of time. Despite being modestly familiar with the morphometric software packages, I often still find it easier and faster to count small amounts of gold particles by eye. I use a program like Photoshop to place a colored dot on top of each gold particle as i click it. My experience is that i can do a lot of images fast and not have to worry about losing gold particles touching black membranes or something that screws up my thresholding detection in a random set of real world images. I can always tweak the thresholding for a single image but often find the next image needs a tad more tweaking. I guess if I was a lot better at image processing or my samples were more idealized, I would agree with John's comment but most people aren't as good as he is so I think there is a place for those of us who count by eye.
At 06:17 PM 09/12/05, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I have followed this discussion with some interest because a similar situation prevails in cathodoluminescence (CL) instrumentation, including those used on optical microscopes (10 to 20 kV, 0-1 mA electron beam).
The beam current is obviously an important variable since the total power is the product of beam voltage and beam current. This can be as much as 10 watts or more in the CL instruments (15 kV and 0.7 mA typical operating condition). One of the questions that comes up immediately is the measurement of the beam current. I am not familiar with the methods of electron microscopy but in CL microscopy often the beam current "displayed" is the total current from the high voltage supply, usually measured in the ground return line. The actual current to the specimen can be significantly less than this because a portion of the beam is intercepted on various anode apertures, collimators, etc., depending on the particular instrument design.
It is possible to measure the current to the specimen if it is set up with a suitable Faraday cup arrangement to suppress secondary electrons but this is rarely done.
Sample temperature discussions often go back to Carslaw and Jaeger, 1959, Conduction of Heat in Solids (general solution for a point source on a semi-infinite solid) and Castaing, R. 1952 (Thesis - Application des sondes electronique a une methode d'analyse ponctuelle chimique et cristallographique.). Castaing's solution is appropriate to electron probe conditions.
With the geological thin sections that are the usual subject for optical CL, the lateral thermal conductivity of the section is often a big question mark also. Tight, well-cemented, samples have relatively high thermal conductivity. But there are examples of quartz sandstones with high porosity where the individual quartz grains in the thin section do not make contact with adjacent grains - only with the epoxy imbedding media. I have seen situations where an individual grain is so well thermally isolated by the epoxy that it would glow brightly with cathodoluminescence and then become "red hot" to the unaided eye while adjacent grains would appear undisturbed..
Don Marshall
Donald J. Marshall (Dr.) RELION Industries PO Box 12 Bedford, MA 01730 USA
With regard to the image analysis problem in hand (TEM and 'black' gold particles), ImageJ (or NIHImage in its Apple variant) is probably the best way to go as it is a powerful image analysis program and free to use. It is a little complicated in its user interface though (not that MetaMorph is actually much better in this respect for the extra £3000). For a stylus input (cell tracing) and a photo holding pad you need to get one of those Wacom Graphire pads for £80 or so (www.wacom.com). I have to say I and many others never got on with the Wacom stylus/pad I bought, but other users in the department seem to love them. I am happier with the mouse. The Graphire tablets work on the Apple and PC, integrate into programs (and also have a mouse).
Find ImageJ (PC) at http://rsb.info.nih.gov/ij/ and NIH Image (Apple) at http://rsb.info.nih.gov/nih-image/. As mentioned PhotoShop can trace round images to give pixel area - but ImageJ is a proper image analysis program and would be useful in other projects. Electron micrographs are often tricky to threshold , so tracing is probably quicker (although try size parameters to remove the larger detected objects, and total area / mean individual object area). When using ImageJ have a good look at all the plug-ins (I download and install any that seem even vaguely interesting). The basic package can do the area measurements and grain counts once calibrated (Analyze, Set Scale) and thresholded. ImageJ also has a selection of image processing commands (but a duff image remains a duff image afterwards).
If you have a had a bit of luck on the gee gee's, try Image Pro whose home is http://www.mediacy.com/ and MetaMorph at http://www.universal-imaging.com/. These programs are pretty slick for most applications, and have lots of extra's like cell motility tracking, but MetaMorph in particular is idiosyncratic and difficult to get to grips with for the casual user (but its mostly all in there somewhere). Useful image processing applications like deconvolution are extra (a lot extra). They are both expensive basic packages as well. OpenLabs at http://www.improvision.com is also OK for this sort of thing, and is Apple based, but its better at image capture and time-lapse as its image analysis component is relatively poor (although their Velocity package is a great 3D reconstruction program at £10,000).
I have been using image analysers since the 1970's Quantimet 720 and I have to say that generally it is often easier to count by eye rather than use the image analyser. This is particularly true for things I count like fibres (complex counting rules) and alpha track stars (that have tracks that vary in number, length and thickness). However if you have hundreds of very bright or very black dots on the image that can be distinguished easily by thresholding then obviously the image analysis program can count them easily. Plus if you are counting hundreds of grains within a sample if doesn't really matter if the image analyser counts 950 and you count 980 - both counts are probably well within biological variation. Just pick the quickest way to count (often by the time you have processed an image for automatic counting often you could have counted two images by eye, particularly if there aren't that many objects). Modern programs should have a manual count option anyway - click on the screen and the object is ticked off, numbered and counted. Also do make yourself a help file document of how you did something with an image analysis package, as its often difficult to remember how you did it a few months on (PrtSC to copy and paste the program menu VDU view is useful).
Older dedicated hardware based image analysers like the UK Magiscan Colour and the Seescan systems were often faster than modern multi-tasking PC versions and included things like real-time binary image editors and light pens (and they never seemed to crash). They also seemed to be more tuned into scientific research needs (e.g. their separate detected object algorithms worked far better in 1989 than those in Metamorph, ImagePro or OpenLabs do now). With any image analysis its the quality of the sample and captured image that is paramount, so its worth spending time on sample preparation and setting up the microscope (e.g. for DIC). Uneven 'illumination' and a poor specimen is a disaster in light microscopy (and EM) image analysis, although background correction can help in light microscopy. Trying to recover information from a poor specimen by image analysis techniques is not the way to go.
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
I am working with calcium carbonate and I am trying to index a single crystal film. Should the relative intensities reported in the JCPDS match the relative intensities in the selected area diffraction pattern.
-- FAIRLAND FONTILLAS AMOS, PhD Materials Science and Engineering University of Florida
==============================Original Headers============================== 3, 21 -- From famos-at-ufl.edu Tue Sep 13 09:16:07 2005 3, 21 -- Received: from smtp.ufl.edu (sp11en1.nerdc.ufl.edu [128.227.74.11]) 3, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8DEG69B021487 3, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2005 09:16:06 -0500 3, 21 -- Received: from osgjas04.cns.ufl.edu (osgjas04.cns.ufl.edu [128.227.74.134]) 3, 21 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j8DEFvmq152860 3, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2005 10:15:57 -0400 3, 21 -- Message-ID: {1662871337.1126620957282.JavaMail.osg-at-osgjas04.cns.ufl.edu} 3, 21 -- Date: Tue, 13 Sep 2005 10:15:57 -0400 (EDT) 3, 21 -- From: "AMOS,FAIRLAND FONTILLAS" {famos-at-ufl.edu} 3, 21 -- To: Microscopy-at-microscopy.com 3, 21 -- Subject: TEM intensity of spots 3, 21 -- MIME-Version: 1.0 3, 21 -- Content-Type: text/plain; format=flowed; charset=us-ascii 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/) 3, 21 -- X-Originating-IP: 10.245.55.249 [10.245.55.249] 3, 21 -- X-Spam-Status: hits=-0.908, required=5, tests=BAYES_10 3, 21 -- X-UFL-Spam-Status: hits=-0.908, required=5, tests=BAYES_10 3, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
-----Original Message----- X-from: Richard Harris [mailto:rjharris-at-uwo.ca] Sent: Tuesday, September 13, 2005 10:12 AM To: paul_hazelton-at-umanitoba.ca
for years i've been able to do the stats on my immunogold quite easily. unfortunately i have a project which requires dealing with odd shaped granules and inclusions in cells, and labeling on the membranes vs not on the membranes. i'm afraid i'm going to have to come into the computer age here, finally.
i would appreciate the advice of the list on simple programs which will allow me to use a stylus to draw around the perimeter of the region in the micrograph which needs to be analysed, and then give me the total area within the region identified. don't need any more analysis. i can still count the black spots - the eyes haven't gone yet.
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
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==============================Original Headers============================== 21, 26 -- From rjharris-at-uwo.ca Tue Sep 13 09:17:47 2005 21, 26 -- Received: from uwo.ca (v320-146-lb.lb.its.uwo.ca [129.100.74.146]) 21, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8DEHkPZ023937 21, 26 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2005 09:17:47 -0500 21, 26 -- Received: from chico.mail.uwo.pri (whelan.mail.uwo.pri [172.29.32.40]) 21, 26 -- by chico.mail.uwo.pri 21, 26 -- (Sun Java System Messaging Server 6.2-2.05 (built Apr 28 2005)) 21, 26 -- with ESMTP id {0IMR0027IEDMO320-at-chico.mail.uwo.pri} for 21, 26 -- microscopy-at-microscopy.com; Tue, 13 Sep 2005 10:17:46 -0400 (EDT) 21, 26 -- Received: from TCU1 ([129.100.68.196]) 21, 26 -- by chico.mail.uwo.pri (Sun Java System Messaging Server 6.2-2.05 (built Apr 28 21, 26 -- 2005)) with SMTP id {0IMR002M8EDMOA00-at-chico.mail.uwo.pri} for 21, 26 -- microscopy-at-microscopy.com; Tue, 13 Sep 2005 10:17:46 -0400 (EDT) 21, 26 -- Date: Tue, 13 Sep 2005 10:15:50 -0400 21, 26 -- From: Richard Harris {rjharris-at-uwo.ca} 21, 26 -- Subject: FW: [Microscopy] image analysis 21, 26 -- To: Microscopy List Server {microscopy-at-microscopy.com} 21, 26 -- Message-id: {ILEAJKBHALKDABDKJAPGAEADDBAA.rjharris-at-uwo.ca} 21, 26 -- MIME-version: 1.0 21, 26 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 21, 26 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2910.0) 21, 26 -- Content-type: text/plain; charset=iso-8859-1 21, 26 -- Content-transfer-encoding: 7BIT 21, 26 -- Importance: Normal 21, 26 -- X-Priority: 3 (Normal) 21, 26 -- X-MSMail-priority: Normal ==============================End of - Headers==============================
I have had success with a free image processing program known as UTHSCSA Image Tool (link below). I have used it in the past to bin and tag varying sizes of pores in ceramic films.
http://ddsdx.uthscsa.edu/dig/itdesc.html
cheers, cj
CJ Bonifas Engineer, Failure Analysis Group Cypress Semiconductor (Minnesota), Inc. 2401 East 86th Street Bloomington, MN, USA 55318
bci-at-cypress.com 952.851.5370
-------- Original Message --------
for years i've been able to do the stats on my immunogold quite easily. unfortunately i have a project which requires dealing with odd shaped granules and inclusions in cells, and labeling on the membranes vs not on the membranes. i'm afraid i'm going to have to come into the computer age here, finally.
i would appreciate the advice of the list on simple programs which will allow me to use a stylus to draw around the perimeter of the region in the micrograph which needs to be analysed, and then give me the total area within the region identified. don't need any more analysis. i can still count the black spots - the eyes haven't gone yet.
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
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No generally not, there are dynamical diffraction effects in electron diffraction that affect the intensity of spots. This includes double diffraction effects that can allow some classes of forbidden reflections (those forbidden by glide planes and screw axis) to occur. There are innumerable other factors that make the intensities different as well.
Use the d-spacings and forget the intensities.
In order to index electron diffraction patterns it really help to have a complete list of all d-spacings and corresponding symmetrically-equivalent hkls for a given material. Such a list needs to be calculated from the cell parameters using appropriate software. I use some home-grown software to do this. I don't know if there is any commercially available software on the market right now that will do it. (Listserver folks help if you know...)
Calcium carbonate has the R3barC space group and will have dynamically allowed and dynamically forbidden reflections depending on whether you calculate the d-spacing based on the primitive rhomobohedral or center hexagonal cell.
Hope this helps.
Roy Christoffersen SAIC NASA Johnson Space Center
-----Original Message----- X-from: famos-at-ufl.edu [mailto:famos-at-ufl.edu] Sent: Tuesday, September 13, 2005 8:21 AM To: rcsaic-at-sbcglobal.net
I am working with calcium carbonate and I am trying to index a single crystal film. Should the relative intensities reported in the JCPDS match the relative intensities in the selected area diffraction pattern.
-- FAIRLAND FONTILLAS AMOS, PhD Materials Science and Engineering University of Florida
==============================Original Headers============================== 3, 21 -- From famos-at-ufl.edu Tue Sep 13 09:16:07 2005 3, 21 -- Received: from smtp.ufl.edu (sp11en1.nerdc.ufl.edu [128.227.74.11]) 3, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8DEG69B021487 3, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2005 09:16:06 -0500 3, 21 -- Received: from osgjas04.cns.ufl.edu (osgjas04.cns.ufl.edu [128.227.74.134]) 3, 21 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j8DEFvmq152860 3, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2005 10:15:57 -0400 3, 21 -- Message-ID: {1662871337.1126620957282.JavaMail.osg-at-osgjas04.cns.ufl.edu} 3, 21 -- Date: Tue, 13 Sep 2005 10:15:57 -0400 (EDT) 3, 21 -- From: "AMOS,FAIRLAND FONTILLAS" {famos-at-ufl.edu} 3, 21 -- To: Microscopy-at-microscopy.com 3, 21 -- Subject: TEM intensity of spots 3, 21 -- MIME-Version: 1.0 3, 21 -- Content-Type: text/plain; format=flowed; charset=us-ascii 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/) 3, 21 -- X-Originating-IP: 10.245.55.249 [10.245.55.249] 3, 21 -- X-Spam-Status: hits=-0.908, required=5, tests=BAYES_10 3, 21 -- X-UFL-Spam-Status: hits=-0.908, required=5, tests=BAYES_10 3, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
==============================Original Headers============================== 15, 20 -- From rcsaic-at-sbcglobal.net Tue Sep 13 09:35:24 2005 15, 20 -- Received: from smtp105.sbc.mail.mud.yahoo.com (smtp105.sbc.mail.mud.yahoo.com [68.142.198.204]) 15, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j8DEZLPW009062 15, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 13 Sep 2005 09:35:23 -0500 15, 20 -- Message-Id: {200509131435.j8DEZLPW009062-at-ns.microscopy.com} 15, 20 -- Received: (qmail 76676 invoked from network); 13 Sep 2005 14:35:19 -0000 15, 20 -- Received: from unknown (HELO STUDYDESKTOP) (rcsaic-at-sbcglobal.net-at-70.240.200.179 with login) 15, 20 -- by smtp105.sbc.mail.mud.yahoo.com with SMTP; 13 Sep 2005 14:35:19 -0000 15, 20 -- From: "Roy Christoffersen" {rcsaic-at-sbcglobal.net} 15, 20 -- To: {famos-at-ufl.edu} , "Microscopy Listserver" {Microscopy-at-Microscopy.Com} 15, 20 -- Subject: RE: [Microscopy] TEM intensity of spots 15, 20 -- Date: Tue, 13 Sep 2005 09:35:19 -0500 15, 20 -- MIME-Version: 1.0 15, 20 -- Content-Type: text/plain; 15, 20 -- charset="us-ascii" 15, 20 -- Content-Transfer-Encoding: 7bit 15, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 15, 20 -- Thread-Index: AcW4blwKxAH+IZzjRbe8rZiTP4R9TQAABCkg 15, 20 -- In-Reply-To: {200509131420.j8DEKvXx003704-at-ns.microscopy.com} 15, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1409 ==============================End of - Headers==============================
"The statements and opinions expressed here by Gary M. Brown represent neither those of ExxonMobil Corporation nor its affiliates."
Tamara,
Ultrathin sections of oriented polymers (stained or unstained) often deform when first exposed to the beam. This relaxation can be achieved prior to analysis by low-dose exposure to the electron beam for several minutes at low magnification. The objective is to relax the sections and make them physically stable during microscopy. I prefer not to use this procedure because it can cause significant deformation of the sections.
A better procedure, in my opinion, is to mount the sections on high quality continuous carbon film grids. Do not use Formvar or Formvar/carbon films since Formvar films are not very clean and can cause problems during imaging and elemental analysis. The sections adhere to the carbon film and will not deform under the beam, thus eliminating artifacts relaxation and/or orientation in images. Image quality is still very good. One must still be careful about beam damage, since this is still a real possibility, carbon film or not.
Hope this helps,
Gary M. Brown ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
thoward-at-unm.ed u To gary.m.brown-at-exxonmobil.com 09/12/05 05:51 cc PM Subject [Microscopy] help from a polymer TEM Please respond person? to thoward-at-unm.ed u
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
One of our TEM service reps told me that he's been told that many people who looks at polymers in the TEM "pre-burn" their samples under a UV lamp before putting the grids in the column - is this true? If so, how is it done (for how long, distance from bulb, etc.)?
We have a weird "thing" with our TEM that burns a pattern into resin sections and we were discussing ways to pre-burn; chemical stetching doesn't help, and doing it in the column is too slow when there are a lot of samples. If I could do a mass burn I'd be set!
Thanks!
Tamara
|--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward-at-unm.edu |--------------------------------------------------|
==============================Original Headers============================== 5, 14 -- From thoward-at-unm.edu Mon Sep 12 17:50:16 2005 5, 14 -- Received: from unm.edu (f5vs2.unm.edu [64.106.76.41]) 5, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8CMoGFU025970 5, 14 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 17:50:16 -0500 5, 14 -- Received: from [129.24.9.43] (HELO deneb.unm.edu) 5, 14 -- by phact.unm.edu (CommuniGate Pro SMTP 4.3.6) 5, 14 -- with ESMTPS id 27235650 for microscopy-at-microscopy.com; Mon, 12 Sep 2005 16:50:14 -0600 5, 14 -- Date: Mon, 12 Sep 2005 16:50:14 -0600 (MDT) 5, 14 -- From: Tamara Howard {thoward-at-unm.edu} 5, 14 -- To: Microscopy Server {microscopy-at-microscopy.com} 5, 14 -- Subject: help from a polymer TEM person? 5, 14 -- Message-ID: {Pine.LNX.4.62.0509121642550.11474-at-deneb.unm.edu} 5, 14 -- MIME-Version: 1.0 5, 14 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 25, 20 -- From gary.m.brown-at-exxonmobil.com Tue Sep 13 09:49:49 2005 25, 20 -- Received: from hoespc10.exxonmobil.com (hoespc10.exxonmobil.com [192.67.48.218]) 25, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8DEnnQF023061 25, 20 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2005 09:49:49 -0500 25, 20 -- Received: from hounmg03.NA.XOM.COM (hounmg03.na.xom.com [158.35.101.41]) 25, 20 -- by hoespc10.exxonmobil.com (Switch-3.1.7/Switch-3.1.7) with ESMTP id j8DEf7pV006756; 25, 20 -- Tue, 13 Sep 2005 09:41:08 -0500 (CDT) 25, 20 -- In-Reply-To: {200509122251.j8CMpmj0029921-at-ns.microscopy.com} 25, 20 -- Subject: Re: [Microscopy] help from a polymer TEM person? 25, 20 -- Importance: 25, 20 -- To: microscopy-at-microscopy.com 25, 20 -- Cc: thoward-at-unm.edu 25, 20 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 25, 20 -- Message-ID: {OF8DE8F4F6.6E277779-ON8625707B.004FAA38-8625707B.0051755D-at-exxonmobil.com} 25, 20 -- From: gary.m.brown-at-exxonmobil.com 25, 20 -- Date: Tue, 13 Sep 2005 09:49:44 -0500 25, 20 -- X-MIMETrack: Serialize by Router on Hounmg03.na.xom.com/S/ExxonMobil(652HF702|December 25, 20 -- 14, 2004) at 09/13/2005 09:49:48 AM 25, 20 -- MIME-Version: 1.0 25, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
There is a new add-in for the analySIS software that does pretty much exactly what you want. It identifies the gold particles not only by their grey level, but also by their "roundness" and expected diameters. You can also analyze double-labeled samples, etc. I don't want to make this too commercial here, so please contact me via email. I am also looking for beta-testers of the software. If you (or anybody else) is interested, please contact me.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I have thought about your inquiry for several hours and waited for anybody to respond . Gary Brown´s advice makes sense.
UV lamp produces oxygen radicals and therefore attacks carbon compounds as a preferred chemical reaction partner. Using a UV-lamp is very slow and results, depend on distance to the source and radiant heat.
Nevertheless, I recommend a plasma treatment before analysis. This could possibly mean cleaning, surface modification and conditioning in one step. Hopefully, you do have a plasma instrument in your lab to test. Try air or bottled oxygen for a start.
Kind Regards Jost
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ GaLa Gabler Labor Instrumente Handels GmbH An der Schmalmach 42 D - 65307 Bad Schwalbach Germany Tel: +49-6124-77 952 Fax: +49-6124-60 274 gala-instrumente-at-t-online.de http://www.gala-instrumente.de/ http://www.plasmainstrument.com/ ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
-----Ursprüngliche Nachricht----- Von: thoward-at-unm.edu [mailto:thoward-at-unm.edu] Gesendet: Dienstag, 13. September 2005 00:54 An: gala-instrumente-at-t-online.de Betreff: [Microscopy] help from a polymer TEM person?
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One of our TEM service reps told me that he's been told that many people who looks at polymers in the TEM "pre-burn" their samples under a UV lamp before putting the grids in the column - is this true? If so, how is it done (for how long, distance from bulb, etc.)?
We have a weird "thing" with our TEM that burns a pattern into resin sections and we were discussing ways to pre-burn; chemical stetching doesn't help, and doing it in the column is too slow when there are a lot of samples. If I could do a mass burn I'd be set!
Thanks!
Tamara
|--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward-at-unm.edu |--------------------------------------------------|
==============================Original Headers============================== 5, 14 -- From thoward-at-unm.edu Mon Sep 12 17:50:16 2005 5, 14 -- Received: from unm.edu (f5vs2.unm.edu [64.106.76.41]) 5, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8CMoGFU025970 5, 14 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2005 17:50:16 -0500 5, 14 -- Received: from [129.24.9.43] (HELO deneb.unm.edu) 5, 14 -- by phact.unm.edu (CommuniGate Pro SMTP 4.3.6) 5, 14 -- with ESMTPS id 27235650 for microscopy-at-microscopy.com; Mon, 12 Sep 2005 16:50:14 -0600 5, 14 -- Date: Mon, 12 Sep 2005 16:50:14 -0600 (MDT) 5, 14 -- From: Tamara Howard {thoward-at-unm.edu} 5, 14 -- To: Microscopy Server {microscopy-at-microscopy.com} 5, 14 -- Subject: help from a polymer TEM person? 5, 14 -- Message-ID: {Pine.LNX.4.62.0509121642550.11474-at-deneb.unm.edu} 5, 14 -- MIME-Version: 1.0 5, 14 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 18, 27 -- From gala-instrumente-at-t-online.de Tue Sep 13 15:40:34 2005 18, 27 -- Received: from mailout03.sul.t-online.com (mailout03.sul.t-online.com [194.25.134.81]) 18, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8DKeY7l012057 18, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2005 15:40:34 -0500 18, 27 -- Received: from fwd30.aul.t-online.de 18, 27 -- by mailout03.sul.t-online.com with smtp 18, 27 -- id 1EFHZu-00048T-03; Tue, 13 Sep 2005 22:40:30 +0200 18, 27 -- Received: from BRO02LAN (VT9LFiZHoe5BKbtzJsxxVsQqy6F7XJj9cdMybaTGBYFB3wefyXQas9-at-[84.169.125.43]) by fwd30.sul.t-online.de 18, 27 -- with smtp id 1EFHZr-2Alc6C0; Tue, 13 Sep 2005 22:40:27 +0200 18, 27 -- From: "GaLa Instrumente GmbH" {gala-instrumente-at-t-online.de} 18, 27 -- To: {thoward-at-unm.edu} 18, 27 -- Cc: {Microscopy-at-microscopy.com} 18, 27 -- Subject: AW: [Microscopy] help from a polymer TEM person? 18, 27 -- Date: Tue, 13 Sep 2005 22:39:59 +0200 18, 27 -- Message-ID: {MPECLIMLPGMGAEKNKJPIOEKBDNAA.gala-instrumente-at-t-online.de} 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain; 18, 27 -- charset="iso-8859-1" 18, 27 -- Content-Transfer-Encoding: 8bit 18, 27 -- X-Priority: 3 (Normal) 18, 27 -- X-MSMail-Priority: Normal 18, 27 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 18, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 18, 27 -- In-reply-to: {200509122254.j8CMs28F001463-at-ns.microscopy.com} 18, 27 -- Importance: Normal 18, 27 -- X-ID: VT9LFiZHoe5BKbtzJsxxVsQqy6F7XJj9cdMybaTGBYFB3wefyXQas9 18, 27 -- X-TOI-MSGID: b8d9af5b-e5ce-4f56-827a-f4c6c9b0b072 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dfine-at-seton.org) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, September 13, 2005 at 14:57:45 ---------------------------------------------------------------------------
Email: dfine-at-seton.org Name: Diann Fine
Organization: Brackenridge Hospital
Education: Graduate College
Location: Austin, Texas USA
Question: I work in the Electron Microscopy area of the histology department.I would appreciate a procedure for liver biopsies. I am using the new ERL-4221,Cycloaliphatic Epoxide Resin from EMS.The thick and thin sections look hazy and cloudy. I wondered if it could be the resin.Do you use it in the same proportions as the old VCD?
Dear Damien (and others who replied to this question),
Thanks for your kind advice.
I expect the double cover-slip procedure will be needed. However for the repeated handling of many samples, it would be good if there was some kind of holder system that made the coverslips easy to handle.
I will probably put the coverslips in a cardboard sandwich with holes.
If it was of interest to many people, perhaps a holder like this could be mass-produced at low cost, with covered strips of adhesive ready-to-go.
Best regards, Peter
*********
} Peter, } } I'm not sure that this is going to work very well if you are talking about a } specimen on a regular microscope slide with a standard cover slip. One } reason is that the slide is much thicker and you may not have sufficient } working distance at higher magnifications unless you are using extra long } working distance lenses. I don't know of any such ELWD oil immersion lenses } for higher magnification work. Furthermore you may not be able to obtain } Koehler illumination. } } The effect of the thicker microscope slide glass on the image is another } issue. I would suggest that you prepare your sample between two large cover } slips in whatever mounting media you are using and tack them together with } either clear tape or a thin coating of clear finger nail polish, or other } adhesive. Then you should be able to flip them over and not worry about one } slipping away from the other and if you are using water, they will not dry } out as quickly. } } Damian Neuberger, Ph.D. } Consultant } Microscopy/Digital Imaging/Image Analysis } 2416 Covert Rd } Glenview IL 60025 } Tel: (847) 998-8574 } email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}
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The intensity in electron diffraction are subject tro many parameters such as doubble diffraction , thisckness of the specimen. So they very often do not match those reported in the JCPDS data base. To indexe you should only consider the position of the reflexions. A programm like "CaRine Crystallography" may help you to do that
A 16:21 13/09/2005, vous avez écrit :
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We're thinking of replacing the polaroid model 545 4x5 film holder in = our Hitachi S-800 SEM with a camera that will allow us to take digital = images instead. Does anyone have any suggestions for models that will = fit our microscope?
Thanks,
David Morgan Department of Chemistry University of Oxford
==============================Original Headers============================== 4, 26 -- From david.morgan-at-christ-church.oxford.ac.uk Wed Sep 14 07:06:38 2005 4, 26 -- Received: from relay0.mail.ox.ac.uk (relay0.mail.ox.ac.uk [129.67.1.161]) 4, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EC6cnS024637 4, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Sep 2005 07:06:38 -0500 4, 26 -- Received: from smtp0.herald.ox.ac.uk ([163.1.0.246]) 4, 26 -- by relay0.mail.ox.ac.uk with esmtp (Exim 4.52) 4, 26 -- id 1EFW29-0006iW-2y 4, 26 -- for Microscopy-at-microscopy.com; Wed, 14 Sep 2005 13:06:37 +0100 4, 26 -- Received: from webmail219.herald.ox.ac.uk ([163.1.0.219]) 4, 26 -- by smtp0.herald.ox.ac.uk with esmtp (Exim 3.35 #1) 4, 26 -- id 1EFW29-00080R-00 4, 26 -- for Microscopy-at-microscopy.com; Wed, 14 Sep 2005 13:06:37 +0100 4, 26 -- Received: by webmail219.herald.ox.ac.uk (Postfix, from userid 101) 4, 26 -- id 9E263226DE; Wed, 14 Sep 2005 13:06:37 +0100 (BST) 4, 26 -- Content-Type: text/plain 4, 26 -- Content-Disposition: inline 4, 26 -- Content-Transfer-Encoding: binary 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Mailer: MIME-tools 5.411 (Entity 5.404) 4, 26 -- From: David Morgan {david.morgan-at-christ-church.oxford.ac.uk} 4, 26 -- Date: Wed, 14 Sep 2005 13:06:37 +0100 (BST) 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Subject: SEM: Digital Camera for Hitachi S-800 4, 26 -- X-Webmail-Originating-Ip: 129.67.69.196 4, 26 -- X-Webmail-Sender: chri0984 4, 26 -- Message-Id: {20050914120637.9E263226DE-at-webmail219.herald.ox.ac.uk} ==============================End of - Headers==============================
If you don't use digital images, you can also use a transparent paper that you place on top of your micrograph. You draw the area you're interested in, you cut it out with cisors and you weight it with a precision scale. After determination of your weight-to-surface factor (just weight a 1 cm2 piece of the same paper), you don't need any computer to determine the surface. It's easy and acurate (and fast).
Re "adhesive strips". Somewhere in the deep dark recesses of my memory, I recall that someone manufactured something that would form a strip of adhesive on a slide onto which a cover slip would be laid. Sort of a transfer system. I think it had to do with cell culture work and created a tiny chamber.
Damian
-----Original Message----- X-from: Peter Matthews [mailto:pjm-at-gol.com] Sent: Tuesday, September 13, 2005 11:32 PM To: Damian Neuberger Cc: Microscopy Listserver
New York Microscopical Society Bernard Friedman Memorial Workshops Use of the Microscope October 1, 8, 15,22 2005 A basic course on light microscopy which will cover the following topics:
Theory of microscopy, Kohler Illumination Diffraction Theory, Contrast Methods Polarized light, Phase Contrast, Interference Hoffman contrast, Rheinberg, Dark-field & oblique Illumination, etc.
The workshop will consist of four consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of microscopy. The course instructors are Jan Hinsch formerly of Leica Microsystems, Inc., Dennis O’Leary of Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of Smiths Detection, Inc. and N.Y.M.S. Instructor Don O'Leary.
WHEN: October 1, 8, 15,22 2005 from 10 A.M. to 4 P.M.
WHERE: 30 N. Mountain Avenue, Montclair, NJ, Phone (973) 744-0043 (parking available, accessible by public transportation. Information on car pools and transportation will be provided.)
COST: $395 for NYMS members, $425 for non-members (includes membership) Lunch and course materials are included. Checks made out to NYMS.
WHO: Beginners and experienced users who wish to learn more about the proper use of a microscope.
HOW: Register using form below. Limited to the first 12 registrants. Send form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.
FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 E-mail: donoleary-at-worldnet.att.net Fax (425) 988-1415
PLEASE POST
------------------------------------------------------------------------- Registration Form Use of the Microscope 2005
The College of Microscopy will be offering a short course, Scanning Electron Microscopy, October 17-21, 2005, at our Westmont Facility. In addition to lectures, the course emphasizes hands-on training using five scanning electron microscopes and electron microprobe analyzers, and gives students the opportunity to work on their own samples. For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 6, 27 -- From eschumacher-at-mccrone.com Wed Sep 14 08:52:30 2005 6, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 6, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EDqTau023929 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 14 Sep 2005 08:52:29 -0500 6, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 6, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 8CA711A8013 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 14 Sep 2005 08:52:29 -0500 (CDT) 6, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 6, 27 -- by pgp.mccrone.com (PGP Universal service); 6, 27 -- Wed, 14 Sep 2005 08:52:29 -0500 6, 27 -- X-PGP-Universal: processed 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="US-ASCII" 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 6, 27 -- Subject: Short Course Announcement: Scanning Electron Microscopy 6, 27 -- Date: Wed, 14 Sep 2005 08:52:17 -0500 6, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C2FC4-at-MCCRONEMSG.tmg.mccrone.com} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Short Course Announcement: Scanning Electron Microscopy 6, 27 -- thread-index: AcW5M4VAaLtzKUJ2Q7ecSdCwpfY23Q== 6, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8EDqTau023929 ==============================End of - Headers==============================
FOCUS ON MICROSCOPY 2006, Perth, Australia, April 9-12, 2006 19th International Conference on 3D Image Processing in Microscopy 18th International Conference on Confocal Microscopy
Dear Colleagues
After the successful FOM2005 conference held in Jena in March this year, it is a pleasure to announce that the next conference: Focus on Microscopy 2006 will take place in Perth, Western Australia, April 9-12, 2006. As the next in a series of unique interdisciplinary meetings on advanced multidimensional light microscopy and image processing, the conference will be hosted by the University of Western Australia in Perth. The conference will be located at the beautiful Esplanade Hotel in the historic waterfront suburb of Perth, Fremantle. Focus on Microscopy 2006 is the continuation of a successful conference series presenting the latest innovations in optical microscopy and its applications in biology, medicine, material science, and information storage. 3D optical imaging and related theory are important subjects for the conference. The series is as relevant now as at any time in its history as the scientific and engineering communities strive to meet the needs of a surging life sciences sector, as well as respond to the sustained pressure for miniaturization in lithography and data storage. The conference series is known for covering the rapid development of advanced fluorescence labelling techniques for the confocal and multi-photon 3D imaging of -live- biological specimens. This year, in addition, special attention will be given to imaging in thick tissues and the use of laser light as an active tool at sub-micrometer length scales for cell biology, nanobiotechnology, and medicine.
Abstracts for contributions are invited and can already be submitted through the website: www.FocusOnMicroscopy.org where further information on the present and previous FOM conferences can be found.
Important dates: Deadline for the submission of abstracts: January 9, 2006 Acceptance of contributions, draft program: January 23, 2006 Deadline for early registration: February 20, 2006
We would be very pleased to welcome you to Perth for the FOM2006 conference and exhibition.
On behalf of the organising committee,
David Sampson, University of Western Australia, Perth, Australia
Fred Brakenhoff Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands
E-mail: info2006-at-FocusOnMicroscopy.org
Web: www.FocusOnMicroscopy.org
==============================Original Headers============================== 15, 29 -- From brakenho-at-science.uva.nl Wed Sep 14 09:11:53 2005 15, 29 -- Received: from imap.science.uva.nl (imap.science.uva.nl [146.50.4.51]) 15, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EEBqCC003524 15, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 14 Sep 2005 09:11:53 -0500 15, 29 -- Received: from webmail.science.uva.nl [146.50.4.91] 15, 29 -- by imap.science.uva.nl with ESMTP (sendmail 8.11.6p2/config 11.35). 15, 29 -- id j8EEBnD26030; Wed, 14 Sep 2005 16:11:49 +0200 15, 29 -- Received: from localhost 15, 29 -- by webmail.science.uva.nl (sendmail 8.11.6/config 11.35). 15, 29 -- id j8EEBmY15181; Wed, 14 Sep 2005 16:11:48 +0200 15, 29 -- X-Organisation: Faculty of Science, University of Amsterdam, The Netherlands 15, 29 -- X-URL: http://www.science.uva.nl/ 15, 29 -- Received: from localhost ([127.0.0.1]) 15, 29 -- (SquirrelMail authenticated user brakenho); 15, 29 -- by webmail.science.uva.nl with HTTP; 15, 29 -- Wed, 14 Sep 2005 16:11:48 +0200 (CEST) 15, 29 -- Message-ID: {55390.62.38.143.95.1126707108.squirrel-at-62.38.143.95} 15, 29 -- Date: Wed, 14 Sep 2005 16:11:48 +0200 (CEST) 15, 29 -- Subject: Focus on Microscopy FOM 2006, Perth, Australia, April 9-12, 2006 15, 29 -- From: "Fred Brakenhoff" {brakenho-at-science.uva.nl} 15, 29 -- To: microscopy-at-msa.microscopy.com 15, 29 -- User-Agent: SquirrelMail/1.4.3a 15, 29 -- X-Mailer: SquirrelMail/1.4.3a 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Type: text/plain;charset=iso-8859-1 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-Priority: 3 (Normal) 15, 29 -- Importance: Normal 15, 29 -- X-Virus-Scanned: by amavisd-new ==============================End of - Headers==============================
The following message is from a former colleague of mine at UConn.
Jim Romanow The University of Connecticut Electron Microscopy Laboratory Physiology and Neurobiology Department Bio-Physics Building, Room G06, Unit 3242 91 North Eagleville Road Storrs, CT 06269-3242 860 486-2914 james.romanow-at-uconn.edu
Dear Jim, I thought that I would forward the job description to you, on the off-chance that you might know someone who was looking for such a position. Any interested parties can follow the link or email their Resume to me, and I can pass it on to my boss. Best regards, Dave Horspool DHorspool-at-FEICO.com
----------------------------------------------------------- Available Job at FEI Company -----------------------------------------------------------
Senior Applications Engineer - TEM (Life Science) Hillsboro, OR
Salary: Open Duration: Full Time Job ID: 112-HBO Post Date: 06/30/2005
Description: The primary responsibility of this position is supporting the sales force and training customers on instrumentation and applications. Secondary responsibilities include acting as a technical resource for sales and service. This will manifest itself in the form of pre-sale visitations to customer sites to explain technical details and give technical presentations, as well as pre-sale system demonstrations either at customer sites or applications laboratories. Service support will be given when certain applications work has to be performed before a system is signed off. This job also involves supporting customers after the sale has been made and the system is signed off.
Key Responsibilities:
* Cultivating positive customer relationships * Act as a high level customer interface for specific instrument/ applications issues * Assisting customers in technique development * Supporting service engineers on instrument sign offs and specific application techniques * Providing feedback to the product groups on system performance, features and problems * Providing input to marketing/development groups for future product developments
This position is ideal for an applicant who wants to be involved with a dynamic, customer, life science market focused team.
Education, Experience and Qualifications:
* The successful candidate will have a degree in the Biological Sciences (Cell and Molecular Biology, Neurobiology). Higher degree is preferred
* 3 years experience in a relevant commercial/research establishment * Excellent communications skills * Well-developed interpersonal skills with a diverse audience * Knowledge of electronic systems/diagrams, as well as complex vacuum systems useful * Diagnostic capabilities as well as faultfinding techniques will be highly valued * Operational experience of analytical equipment; specifically transmission electron microscopes, various energy dispersive spectrometers and Cryo techniques a plus
* Excellent working knowledge and confidence with PC platforms, especially Windows 2000 as well as 3D reconstruction/ Tomography programs and Power Point skills is highly desirable.
Suitable candidate must be willing and able to travel both domestically as well as internationally and must possess a valid passport.
Required Skills: FEI Company is an Equal Opportunity Employer
Have you considered a "screen capture" system that will take the video and store the image? It just may be cheaper. The one caveat about this approach is that you may need to cut into the appropriate points in the video drive circuit since many older microscopes do not bring these points out to a connector. My experience was with a Hitachi S570 and it was a little messy but it did work. My need was to do EDX mapping so the video capture was an integral part of the x-ray system.
Regards,
Peter Tomic
Ciclon Semiconductor Device Corporation
-----Original Message----- X-from: david.morgan-at-christ-church.oxford.ac.uk [mailto:david.morgan-at-christ-church.oxford.ac.uk] Sent: Wednesday, September 14, 2005 8:13 AM To: ptomic-at-ciclonsemi.com
Hello Everyone,
We're thinking of replacing the polaroid model 545 4x5 film holder in = our Hitachi S-800 SEM with a camera that will allow us to take digital = images instead. Does anyone have any suggestions for models that will = fit our microscope?
Thanks,
David Morgan Department of Chemistry University of Oxford
==============================Original Headers============================== 4, 26 -- From david.morgan-at-christ-church.oxford.ac.uk Wed Sep 14 07:06:38 2005 4, 26 -- Received: from relay0.mail.ox.ac.uk (relay0.mail.ox.ac.uk [129.67.1.161]) 4, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EC6cnS024637 4, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Sep 2005 07:06:38 -0500 4, 26 -- Received: from smtp0.herald.ox.ac.uk ([163.1.0.246]) 4, 26 -- by relay0.mail.ox.ac.uk with esmtp (Exim 4.52) 4, 26 -- id 1EFW29-0006iW-2y 4, 26 -- for Microscopy-at-microscopy.com; Wed, 14 Sep 2005 13:06:37 +0100 4, 26 -- Received: from webmail219.herald.ox.ac.uk ([163.1.0.219]) 4, 26 -- by smtp0.herald.ox.ac.uk with esmtp (Exim 3.35 #1) 4, 26 -- id 1EFW29-00080R-00 4, 26 -- for Microscopy-at-microscopy.com; Wed, 14 Sep 2005 13:06:37 +0100 4, 26 -- Received: by webmail219.herald.ox.ac.uk (Postfix, from userid 101) 4, 26 -- id 9E263226DE; Wed, 14 Sep 2005 13:06:37 +0100 (BST) 4, 26 -- Content-Type: text/plain 4, 26 -- Content-Disposition: inline 4, 26 -- Content-Transfer-Encoding: binary 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Mailer: MIME-tools 5.411 (Entity 5.404) 4, 26 -- From: David Morgan {david.morgan-at-christ-church.oxford.ac.uk} 4, 26 -- Date: Wed, 14 Sep 2005 13:06:37 +0100 (BST) 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Subject: SEM: Digital Camera for Hitachi S-800 4, 26 -- X-Webmail-Originating-Ip: 129.67.69.196 4, 26 -- X-Webmail-Sender: chri0984 4, 26 -- Message-Id: {20050914120637.9E263226DE-at-webmail219.herald.ox.ac.uk} ==============================End of - Headers==============================
==============================Original Headers============================== 17, 28 -- From ptomic-at-ciclonsemi.com Wed Sep 14 09:37:30 2005 17, 28 -- Received: from smtp41.mailnet.ptd.net (smtp41.mailnet.ptd.net [204.186.29.21]) 17, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EEbU9s020632 17, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Sep 2005 09:37:30 -0500 17, 28 -- Message-Id: {200509141437.j8EEbU9s020632-at-ns.microscopy.com} 17, 28 -- Received: (qmail 2502 invoked by uid 50005); 14 Sep 2005 14:37:29 -0000 17, 28 -- Received: from 24.229.48.13 by smtp41.mailnet.ptd.net (envelope-from {ptomic-at-ciclonsemi.com} , uid 50002) with qmail-scanner-1.23 17, 28 -- (uvscan: v4.4.00/v4578. 17, 28 -- Clear:RC:0(24.229.48.13):. 17, 28 -- Processed in 0.693085 secs); 14 Sep 2005 14:37:29 -0000 17, 28 -- Received: from unknown (HELO ciclon7a764099) (authenticated:ptomic-at-[24.229.48.13]) 17, 28 -- (envelope-sender {ptomic-at-ciclonsemi.com} ) 17, 28 -- by smtp41.mailnet.ptd.net (qmail-ldap-1.03) with SMTP 17, 28 -- for {david.morgan-at-christ-church.oxford.ac.uk} ; 14 Sep 2005 14:37:28 -0000 17, 28 -- From: "Peter Tomic" {ptomic-at-ciclonsemi.com} 17, 28 -- To: {david.morgan-at-christ-church.oxford.ac.uk} 17, 28 -- Cc: {Microscopy-at-microscopy.com} 17, 28 -- Subject: RE: [Microscopy] SEM: Digital Camera for Hitachi S-800 17, 28 -- Date: Wed, 14 Sep 2005 10:38:01 -0400 17, 28 -- MIME-Version: 1.0 17, 28 -- Content-Type: text/plain; 17, 28 -- charset="us-ascii" 17, 28 -- Content-Transfer-Encoding: 7bit 17, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 28 -- In-Reply-To: {200509141212.j8ECCYvV032056-at-ns.microscopy.com} 17, 28 -- Thread-Index: AcW5JZl99Ia0y1z5RRqUDWaZPmEdeAAE5QpA 17, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 17, 28 -- X-Qmail-Scanner-Message-ID: {11267086496762491-at-smtp41.mailnet.ptd.net} ==============================End of - Headers==============================
From nearly 40 years of working with electron and x-ray diffraction patterns, plus several years of membership in the JCPDS, now ICDD, I can offer a rule of thumb regarding intensity of ediff vs. x-ray diffraction data: Strong reflections are strong reflections and weak are weak. One cannot make an identification of an unknown phase using ediff ring data where very strong x-ray lines are missing from one's pattern, without giving crystal-chemical reasons to account for the missing reflections. Likewise, a 5% intensity x-ray line will not suddenly become a 100% intensity ediff ring-pattern reflection.
Electron diffraction patterns will SOMETIMES have extra and structure factor forbidden spots (& rings, as appropriate) due to double diffraction and relaxation of structure factor rules due to specimen thickness effects with thin TEM specimens, etc. --Emphasis on "sometimes."-- With regard to solving for unknown phases using ediff data, the extra spots/rings, when present, are either a help or a hindrance as they are most conspicuously present at large d-values, which are the most diagnostic d-values for phase identification. In the rare instances where I had a true unknown specimen in the TEM, and I thought I had a match with a phase in the ICDD x-ray data base, except for the presence of weak, large d-spacing reflections in the ediff data, I could sometimes confirm my identification by computing forbidden (100), (110), etc. reflections and matching them to my experimental data. Should an image of your specimen show it to be loaded with twins or other features that cause extra reflections, you should make appropriate forbidden reflection calculations early-on.
The ICDD has products to aid electron diffractionists. The Max-d/Alphabetical Index (now called the Long-d index, I think), and products derived from the Sandia database come to mind. Check www.icdd.com.
Fairland: the answer to your question is "Yes, probably."
Ron Anderson
Disclaimer: I am an emeritus member and a fellow of the ICDD with no direct interaction with them for several years now.
famos-at-ufl.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 20 -- From randerson20-at-tampabay.rr.com Wed Sep 14 10:11:18 2005 11, 20 -- Received: from ms-smtp-05.tampabay.rr.com (ms-smtp-05-smtplb.tampabay.rr.com [65.32.5.135]) 11, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EFBI1R029606 11, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 Sep 2005 10:11:18 -0500 11, 20 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 11, 20 -- by ms-smtp-05.tampabay.rr.com (8.12.10/8.12.7) with ESMTP id j8EFBDeQ008837; 11, 20 -- Wed, 14 Sep 2005 11:11:15 -0400 (EDT) 11, 20 -- Message-ID: {43283D8F.2020809-at-tampabay.rr.com} 11, 20 -- Date: Wed, 14 Sep 2005 11:11:11 -0400 11, 20 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 11, 20 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 11, 20 -- X-Accept-Language: en-us, en 11, 20 -- MIME-Version: 1.0 11, 20 -- To: famos-at-ufl.edu, Listserver {Microscopy-at-MSA.Microscopy.Com} 11, 20 -- Subject: Re: [Microscopy] TEM intensity of spots 11, 20 -- References: {200509131416.j8DEGGlE021712-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {200509131416.j8DEGGlE021712-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
Please join your fellow NESM members for their Fall 2005 meeting to be held at Worcester Polytechnic Institute (WPI) in Worcester, MA on Wednesday, October 12th. Don't forget to invite your colleagues!
The meeting will be held in the historic Higgins House on Wing Road. Registration for this meeting will be $25.00 which will include a reception featuring a beer/wine bar and dinner.
Registration will be from 5:00-6:00pm, followed by a reception/dinner from 5:30-7:00pm. The technical presentations will begin at 7:00pm.
We have 3 exciting speakers scheduled: Philip Klausmeyer of the Worcester Art Museum who will speak on applications of microscopy in art conservation; Michael Jercinovic of the Department of Geosciences at UMASS, Amherst who will present new techniques in x-ray microanalysis, and WPI's own Eric Overstrom from the Department of Biology and Biotechnology who will give us insight in visualizing the key enablers of oocyte developmental competence.
For a map and directions to Higgins House click http://nesm.cims.harvard.edu/Misc/wpidirections.htm
For the full meeting program, together with abstracts and biosketches of the speakers, click http://nesm.cims.harvard.edu/Misc/program.htm
PRE-REGISTRATION IS REQUIRED! Dan Gibson of WPI is Chair of this meeting and he needs a head count for dinner. Please send a check, made out to NESM for $25.00, by Friday, October 7th to: Paul Bain, NESM Treasurer, Countway 212, 10 Shattuck Street, Boston, MA 02115. (email: Paul_Bain-at-hms.harvard.edu )
We hope to see you there!
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
==============================Original Headers============================== 20, 23 -- From MSHERWOOD-at-PARTNERS.ORG Wed Sep 14 12:34:10 2005 20, 23 -- Received: from PHSXCON1.partners.org (phsxcon1.mgh.harvard.edu [132.183.130.40]) 20, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EHYAPX007257 20, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Sep 2005 12:34:10 -0500 20, 23 -- Received: from PHSXMB1.partners.org ([132.183.118.117]) by PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.211); 20, 23 -- Wed, 14 Sep 2005 13:34:05 -0400 20, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 20, 23 -- Content-class: urn:content-classes:message 20, 23 -- MIME-Version: 1.0 20, 23 -- Content-Type: text/plain; 20, 23 -- charset="iso-8859-1" 20, 23 -- Subject: [Microscopy] New England Society for Microscopy October 12th Meeting 20, 23 -- Date: Wed, 14 Sep 2005 13:34:05 -0400 20, 23 -- Message-ID: {1AF23D0AD12E7444A5DB083CA978B73403F92680-at-PHSXMB1.partners.org} 20, 23 -- X-MS-Has-Attach: 20, 23 -- X-MS-TNEF-Correlator: 20, 23 -- Thread-Topic: [Microscopy] New England Society for Microscopy October 12th Meeting 20, 23 -- Thread-Index: AcW5UoEDyqz/oNfjT9SNK0eO0I8OqA== 20, 23 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 20, 23 -- To: {Microscopy-at-microscopy.com} 20, 23 -- X-OriginalArrivalTime: 14 Sep 2005 17:34:05.0775 (UTC) FILETIME=[814DE5F0:01C5B952] 20, 23 -- Content-Transfer-Encoding: 8bit 20, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8EHYAPX007257 ==============================End of - Headers==============================
Hi there, We're setting up a vintage instrument, a Cambridge S-250 with a vintage microspec WD spectrometer.
This spectrometer needs P10, which is to be expected, but also P90. Praxair don't know what this is, logic dictates that its the inverse composition of P10 i.e. 90% methane 10% argon. Do anyone have a similar instrument and knowledge of this gas ? Thanks, Skage
What do other universities, etc. charge for using a Laser Capture Microdissection System? We are starting to have outside users on the system.
Thanks! Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
==============================Original Headers============================== 5, 23 -- From MSHERWOOD-at-PARTNERS.ORG Wed Sep 14 15:37:20 2005 5, 23 -- Received: from PHSXCON3.partners.org (phsxcon3.mgh.harvard.edu [132.183.130.41]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EKbJ8m025076 5, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Sep 2005 15:37:20 -0500 5, 23 -- Received: from PHSXMB1.partners.org ([132.183.118.117]) by PHSXCON3.partners.org with Microsoft SMTPSVC(6.0.3790.211); 5, 23 -- Wed, 14 Sep 2005 16:37:19 -0400 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="iso-8859-1" 5, 23 -- Subject: [Microsocpy] re: LCM fees 5, 23 -- Date: Wed, 14 Sep 2005 16:37:19 -0400 5, 23 -- Message-ID: {1AF23D0AD12E7444A5DB083CA978B73403F9268F-at-PHSXMB1.partners.org} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: [Microsocpy] re: LCM fees 5, 23 -- Thread-Index: AcW5bBnXlgDSaaolTnmBqy3prSbUUw== 5, 23 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 5, 23 -- To: {Microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 14 Sep 2005 20:37:19.0776 (UTC) FILETIME=[1A3D5200:01C5B96C] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8EKbJ8m025076 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pmccurdy-at-lamar.colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, September 14, 2005 at 11:02:37 ---------------------------------------------------------------------------
Email: pmccurdy-at-lamar.colostate.edu Name: Pat McCurdy
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, Colorado, USA
Question: I am interested if anyone on this list has experience upgrading Vantage 2.4 software for Window NT on the ThermoElectron Noran EDS dector to Windows 2000 or Windows XP?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (km602223-at-comcast.net) from http://microscopy.com/MLFormMail.html on Wednesday, September 14, 2005 at 18:36:15 ---------------------------------------------------------------------------
Question: If anyone has experience with the old Nikon Fluophot, is it possible to remove/replace the epi-fluorescence filters in the turret or were they glued in place?
Skage, Yes, P-90 is 90% methane and 10% argon (and is flammable). Sounds like you've got a thin window detector running at low absolute pressure for light elements. That setup needs a lot of quenching gas (methane) and not much ionizing gas (argon) to work well.
Ken Converse
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-----Original Message----- X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca] Sent: Wednesday, September 14, 2005 3:45 PM To: kenconverse-at-qualityimages.biz
Hi there, We're setting up a vintage instrument, a Cambridge S-250 with a vintage microspec WD spectrometer.
This spectrometer needs P10, which is to be expected, but also P90. Praxair don't know what this is, logic dictates that its the inverse composition of P10 i.e. 90% methane 10% argon. Do anyone have a similar instrument and knowledge of this gas ? Thanks, Skage
==============================Original Headers============================== 8, 17 -- From SHem-at-laurentian.ca Wed Sep 14 14:40:56 2005 8, 17 -- Received: from luadmn2.laurentian.ca (mail-backup.laurentian.ca [142.51.1.226] (may be forged)) 8, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8EJeuLI008476 8, 17 -- for {microscopy-at-microscopy.com} ; Wed, 14 Sep 2005 14:40:56 -0500 8, 17 -- Received: from OUTMAIL-MTA by luadmn2.laurentian.ca 8, 17 -- with Novell_GroupWise; Wed, 14 Sep 2005 15:40:51 -0400 8, 17 -- Message-Id: {s3284483.078-at-luadmn2.laurentian.ca} 8, 17 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 8, 17 -- Date: Wed, 14 Sep 2005 15:40:25 -0400 8, 17 -- From: "Skage Hem" {SHem-at-laurentian.ca} 8, 17 -- To: {microscopy-at-microscopy.com} 8, 17 -- Subject: Gas for WD spectrometer 8, 17 -- Mime-Version: 1.0 8, 17 -- Content-Type: text/plain; charset=US-ASCII 8, 17 -- Content-Disposition: inline 8, 17 -- Content-Transfer-Encoding: 8bit 8, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8EJeuLI008476 ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 23 -- From kenconverse-at-qualityimages.biz Wed Sep 14 20:27:40 2005 25, 23 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 25, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8F1RdjT022489 25, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 Sep 2005 20:27:40 -0500 25, 23 -- Received: from Ken [70.200.100.24] by qualityimages.biz with ESMTP 25, 23 -- (SMTPD32-8.05) id AE0A11F30080; Wed, 14 Sep 2005 18:27:38 -0700 25, 23 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 25, 23 -- To: {SHem-at-laurentian.ca} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 25, 23 -- Subject: RE: [Microscopy] Gas for WD spectrometer 25, 23 -- Date: Wed, 14 Sep 2005 21:27:03 -0400 25, 23 -- Message-ID: {002601c5b994$9c29ca50$3c79c846-at-Ken} 25, 23 -- MIME-Version: 1.0 25, 23 -- Content-Type: text/plain; 25, 23 -- charset="us-ascii" 25, 23 -- X-Priority: 3 (Normal) 25, 23 -- X-MSMail-Priority: Normal 25, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 25, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 25, 23 -- Importance: Normal 25, 23 -- In-Reply-To: {200509141944.j8EJisYR014751-at-ns.microscopy.com} 25, 23 -- X-IMSTrailer: __IMail_7__ 25, 23 -- Content-Transfer-Encoding: 8bit 25, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8F1RdjT022489 ==============================End of - Headers==============================
I kind of assumed that anyone using a TEM photo or negative would scan it into a PC at hi-res and then use digital zoom [if necessary] with a stylus+tablet or mouse within the image analysis program to draw round the digitised image (I've bonded with my PC and mouse). I have just had a discussion with Tobias (Baskin) of the University of Massachusetts Biology Department, and he mentioned how he finds it a bit quicker using a tablet mouse with a crosshair to accurately trace round and digitise the photomicrograph's or photocopy's features. I notice that Wacom (www.wacom.co.uk) have a (CAD) crosshair mouse with their Intuos 2 (A4 oversize) tablets and GTCO CalComp make even flasher tablet devices, e.g. the Drawing board III, for a comparable price (Tobias successfully uses a CalComp tablet) - see http://www.gtcocalcomp.com/files/BROdb3small.pdf .
I liked the trace, cut out and weigh idea. It certainly works Ok (if you don't have 500 objects to measure per image). I have also known less numerate colleagues using string to measure perimeter (even for circles). Conversely I have used an epidiascope to capture a graph and a £100k image analyser to measure the area under the curve (then FigP came along in the 1980's).
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {yschwab-at-titus.u-strasbg.fr} To: {keith.morris-at-ucl.ac.uk} Sent: Wednesday, September 14, 2005 1:45 PM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (icmicroanalysis-at-cox.net) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 15, 2005 at 14:14:08 ---------------------------------------------------------------------------
Email: icmicroanalysis-at-cox.net Name: Dave
Title-Subject: [Filtered] SEM Sectioning Tools
Question: Anyone willing to provide feedback on experiences (and recommendations) using either the Allied Cross Sectioning Tool, South Bay BiPod Polisher, or Accelerated Analysis Cross Sectioning Fixture for silicon die cross sectioning?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jvtaylo-at-emory.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 15, 2005 at 16:15:04 ---------------------------------------------------------------------------
Email: jvtaylo-at-emory.edu Name: Jeannette Taylor
Organization: IM&MF/ Emory University
Title-Subject: [Filtered] JEOL load box
Question: Does anyone have a used film canister (load box) for a JEOL JEM 1210 TEM which they are willing to sell or give away? Must be in good condition. Please reply off line.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dawn.dawson-at-case.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 16, 2005 at 08:22:25 ---------------------------------------------------------------------------
Email: dawn.dawson-at-case.edu Name: Dawn Dawson
Organization: Case Western Reserve University
Title-Subject: [Filtered] MListserver: Digital camera for small animal imaging
Question: I am looking for a good digital camera that can take close up gross photos of tumors in mice.... The options for stereomicroscope set-ups are a bit pricey for my pocketbook..and not so flexible...We are currently investigating a Nikon D50 with 60 mm lens and 2.8D...Any suggestions would be appreciated...
This position is not actually in microscopy, but I hope it is close enough that it may interest some subscribers:
The Center for Materials Science and Engineering at the Massachusetts Institute of Technology has a vacancy, with effect from November 1st. 2005, for a research specialist in X-ray Diffraction. A description of the vacancy, together with MIT's employment policies, benefits, and other information, with links for on-line application is available at the following URL:
http://sh.webhire.com/servlet/av/jd?ai=631&ji=1651388&sn=I or applications may be forwarded by electronic or paper mail to Ms. Jennifer Crockett, CMSE Assistant Director, Room # 13-2106, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307, e-mail crockett-at-mit.edu.
The application review process will be open until a qualified candidate has been identified. Questions about this position may be addressed to the undersigned.
Tony Garratt-Reed
*********************************************** Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
The question is primarily aimed at the Biological TEM crowd.
Has anyone experimented with using a Heat block unit for curing plastic instead of an oven?
The reasoning is that a heat block fits in the hood more easily and can be moved out when not in use. The hood space here is extremely limited and the oven typically has been curing Spurr's and Epon type Resins in the prep room, a practice I am not comfortable with (and neither are the Safety folks here).
Looking at the specifications for the Dry Heat Blocks some are +/- 2ºC stability wise in the $200 range with the fanciest models having +/- 0.5ºC range stability.
Yes I am aware of the limitations, specifically sample number, no it wouldn't work for constant routine samples where there's a constant flux, but for individual processes where no more than one or two groups of samples are cured at a time it would seem reasonable.
Thoughts? Experiences? Conjecture? Opinions?
Thanks, Geoff Williams Facility Manager Leduc Bioimaging Facility Brown University
I have never done it but suspect it would work. maybe even better than an oven. Sus Ito, one of the great early TEM guys, once told me how he use to drive from Woods Hole back to Harvard in Boston and he would tape his tissue samples in liquid resin to his engine block so that he could section them upon his arrival! I just wonder how you write the Materials and Methods description of that up!
At 10:05 AM 09/16/05, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Take a look at D100. Also consider the 105/2.8 AF-D micro Nikkor and the SB-29s macro speedlight.
gary g.
At 06:51 AM 9/16/2005, you wrote:
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==============================Original Headers============================== 8, 25 -- From gary-at-gaugler.com Fri Sep 16 10:23:12 2005 8, 25 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j8GFNCOa029481 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 10:23:12 -0500 8, 25 -- Received: (qmail 448 invoked from network); 16 Sep 2005 08:23:10 -0700 8, 25 -- Received: by simscan 1.1.0 ppid: 32603, pid: 32606, t: 20.8991s 8, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1082 spam: 3.0.3 8, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 8, 25 -- by qsmtp3 with SMTP; 16 Sep 2005 08:22:49 -0700 8, 25 -- Message-Id: {6.2.3.4.2.20050916082115.02903aa0-at-mail.calweb.com} 8, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 8, 25 -- Date: Fri, 16 Sep 2005 08:22:52 -0700 8, 25 -- To: dawn.dawson-at-case.edu 8, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 25 -- Subject: Re: [Microscopy] viaWWW: Digital camera for small animal 8, 25 -- imaging 8, 25 -- Cc: microscopy-at-microscopy.com 8, 25 -- In-Reply-To: {200509161351.j8GDp10E020421-at-ns.microscopy.com} 8, 25 -- References: {200509161351.j8GDp10E020421-at-ns.microscopy.com} 8, 25 -- Mime-Version: 1.0 8, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 8, 25 -- X-Spam-Level: 8, 25 -- X-Spam-Status: No, score=-2.4 required=5.0 tests=AWL,BAYES_00 autolearn=ham 8, 25 -- version=3.0.3 ==============================End of - Headers==============================
I apologise for stating the obvious, but have you thought about purchasing a more compact oven from one of the e.m. suppliers. These would at least be capable of holding flat embedding moulds as well as capsules at a uniform temperature.
We purchased one with external dimensions of 400mm x 330mm x 300mm although there was a more compact version of 335mm x 305mm x 230mm in the UK.
I certainly agree with your concerns about polymerising Spurrs/any epoxides resins in the lab. I stopped doing it over 20 years ago. One other possibility would be to find a well vented outhouse/shed if your safety people would be happier with that.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences University of Sunderland Tyne & Wear SR1 3SD UK
----- Original Message ----- X-from: Geoffrey_Williams-at-brown.edu
The Nikon D70 has most (all?) of the features of the D100 (and some additional ones?) at a less-than-$1000 price tag. I've had good experience using it for macro photography, combined with the 60mm macro lens (with an effective focal length of about 85mm) and a Nikon SB600 strobe. The 105mm macro lens (with an effective focal length of about 150mm) will give more working distance, but is a bit harder to hand-hold (this may not be a problem if you put it on a stand). --Jan Factor
--------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College, State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
gary-at-gaugler.com wrote:
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==============================Original Headers============================== 6, 20 -- From jfactor-at-ns.purchase.edu Fri Sep 16 10:52:20 2005 6, 20 -- Received: from zephyr.ns.purchase.edu (zephyr.ns.purchase.edu [199.79.168.193]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8GFqJIR014361 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 16 Sep 2005 10:52:19 -0500 6, 20 -- Received: from ns.purchase.edu ([10.52.0.64]) 6, 20 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0) with ESMTP id j8GFsJX22956; 6, 20 -- Fri, 16 Sep 2005 11:54:19 -0400 6, 20 -- Message-ID: {432AEA1C.5090409-at-ns.purchase.edu} 6, 20 -- Date: Fri, 16 Sep 2005 11:51:56 -0400 6, 20 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.6) Gecko/20040113 6, 20 -- X-Accept-Language: en-us, en 6, 20 -- MIME-Version: 1.0 6, 20 -- To: gary-at-gaugler.com 6, 20 -- CC: "'Microscopy list'" {Microscopy-at-MSA.Microscopy.Com} 6, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Digital camera for small animal 6, 20 -- References: {200509161523.j8GFNrAX031125-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200509161523.j8GFNrAX031125-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The D-50 is a good choice with the 60mm Micro Nikkor f2.8. I have the D2X and same lens for close up macrophotography of flowers and other plant parts for the Chicago Botanic Garden and it is superb but the raw files are 70MB per image! What ever camera you buy, be sure that it can take raw images. Also, if you decide to buy a fixed lens camera, be sure that it is the kind that has a LCD viewfinder in addition to the LCD screen in the back of the camera. For close up photography, you want to see what the lens sees and the LCD on the back of the camera is often hard to see in room light (and outdoors); you can buy hoods but that's another item.
However, there is another issue and that will be lighting. Using photo flood lamps, halogen or other "always on" illumination produce a lot of heat and specimens can quickly dry out and the heat can get to you in a room. Been there done that! One choice for the Nikon is two SB800 flash units mounted on a bracket on either side of the lens. That should give you auto exposure. Another option (and perhaps better) is the ring flash but Nikon's is not auto exposure (yet) but Sigma Photo makes one that is supposed to be auto with the Nikon digital cameras but you should contact Sigma http://www.sigmaphoto.com/flashes/flashes_flashes_details.asp?id=3258 to discuss.
I don't know how close you will have to get to photograph those tumors but if you will have to be inches from the specimen, the auto flash will become a problem in that you may have to start using ND filters. Another option might be to use the 105 mm Micro Nikkor which will give you a little more working distance but it costs more.
Disclaimer: Nikon is only my preference but other manufacturers make equally suitable cameras.
Title-Subject: [Filtered] MListserver: Digital camera for small animal imaging
Question: I am looking for a good digital camera that can take close up gross photos of tumors in mice.... The options for stereomicroscope set-ups are a bit pricey for my pocketbook..and not so flexible...We are currently investigating a Nikon D50 with 60 mm lens and 2.8D...Any suggestions would be appreciated...
==============================Original Headers============================== 14, 23 -- From neuberger1234-at-comcast.net Fri Sep 16 13:18:54 2005 14, 23 -- Received: from rwcrmhc12.comcast.net (rwcrmhc13.comcast.net [204.127.198.39]) 14, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8GIIrWV024772 14, 23 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 13:18:53 -0500 14, 23 -- Received: from personal73sg05 (c-67-167-236-68.hsd1.il.comcast.net[67.167.236.68]) 14, 23 -- by comcast.net (rwcrmhc13) with SMTP 14, 23 -- id {2005091618185201500o5ri9e} ; Fri, 16 Sep 2005 18:18:52 +0000 14, 23 -- From: "Damian Neuberger" {neuberger1234-at-comcast.net} 14, 23 -- To: "Microscopy-at-Microscopy. Com" {microscopy-at-microscopy.com} , 14, 23 -- {dawn.dawson-at-case.edu} 14, 23 -- Subject: RE: [Microscopy] viaWWW: Digital camera for small animal imaging 14, 23 -- Date: Fri, 16 Sep 2005 13:18:51 -0500 14, 23 -- Message-ID: {OKEJIDCNBDPPLLHANALIKEBADBAA.neuberger1234-at-comcast.net} 14, 23 -- MIME-Version: 1.0 14, 23 -- Content-Type: text/plain; 14, 23 -- charset="iso-8859-1" 14, 23 -- Content-Transfer-Encoding: 7bit 14, 23 -- X-Priority: 3 (Normal) 14, 23 -- X-MSMail-Priority: Normal 14, 23 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.6604 (9.0.2911.0) 14, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 14, 23 -- In-Reply-To: {200509161346.j8GDk91n017149-at-ns.microscopy.com} 14, 23 -- Importance: Normal ==============================End of - Headers==============================
You ask a question which is actually quite interesting, and applicable on a number of levels. First, of course, is would a block work. I will assume you mean something like the sand blocks we use in the other (one of my 2 non-EM homes) lab. As you note, there would be limitations, but the temperature control on all sand blocks I’ve ever worked with is a lot better than any oven I’ve used. You just have to take the time to set the temperature. As Tom Phillips noted, there are plenty of examples of alternative systems for providing the polymerization temperatures, so to join the chorus, I see no reason why not. And as far as using the car engine, I even remember a book about cooking while you drive which came out in the 70's, engine block potroast and all.
The interesting part of the question as I see it is: why would you want to put the block into a hood. Do you mean a fume hood, so you could use the block for an intermediate step in infiltration, with low temperature heating to assist in driving off transitional solvents? If so, it is an interesting idea, one worth some thought. Could be quite useful.
Alternatively, do you mean a laminar flow hood for containment at a BSL-2 level or higher. This is the most interesting potential application. Those of us who work with emerging diseases groups, or with infectious pathogens at the BSL-3 or BSL-4 levels are confronted with a number of safety issues that this concept could address. Some of my collaborators work at higher levels. They fix with a modification of Karnovsky’s fix (we use 2% paraform/2%glut in cacodylate). But their safety people will not let them take the material out of containment for further processing until the samples have been in the fixative for 30 days. I feel this may lead to some deterioration of the samples, and that there is no evidence that the pathogens are not inactivated in hours, and so do not like this. But safety people will not let them do otherwise.
There are too many other things to do these days for me to give up 4-6 hour blocks to go work in containment, so I’m not too keen on taking spacesuit training - it would be fun and really interesting, but there is just not enough time. Your question raises a lot of ideas which can address the problems of processing, permit good technique in processing, and meet the demands on the biosafety level.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 9, 19 -- From paul_hazelton-at-umanitoba.ca Fri Sep 16 13:50:14 2005 9, 19 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 9, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8GIoE2H001127 9, 19 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 13:50:14 -0500 9, 19 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 9, 19 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id j8GIo6fh004532; 9, 19 -- Fri, 16 Sep 2005 13:50:06 -0500 (CDT) 9, 19 -- Message-ID: {432B13DF.C669EB0A-at-umanitoba.ca} 9, 19 -- Date: Fri, 16 Sep 2005 13:50:08 -0500 9, 19 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 9, 19 -- X-Mailer: Mozilla 4.78 [en] (Win98; U) 9, 19 -- X-Accept-Language: en,pdf 9, 19 -- MIME-Version: 1.0 9, 19 -- To: Geoffrey_Williams-at-brown.edu 9, 19 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 9, 19 -- Subject: Re: [Microscopy] Curing TEM Epoxy in Heat Blocks? 9, 19 -- References: {200509161506.j8GF6xh3016357-at-ns.microscopy.com} 9, 19 -- Content-Type: text/plain; charset=iso-8859-1 9, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
The macro lenses work best with the macro ring flash SB-29s. It costs about the same as two regular speed lights. However, its big advantage is adjustable power and adjustable shades over the flash lamps.
gary g.
At 08:54 AM 9/16/2005, you wrote:
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==============================Original Headers============================== 9, 24 -- From gary-at-gaugler.com Fri Sep 16 14:43:05 2005 9, 24 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j8GJh554010256 9, 24 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 14:43:05 -0500 9, 24 -- Received: (qmail 8703 invoked from network); 16 Sep 2005 12:43:02 -0700 9, 24 -- Received: by simscan 1.1.0 ppid: 8639, pid: 8642, t: 5.8979s 9, 24 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1082 spam: 3.0.3 9, 24 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 24 -- by qsmtp3 with SMTP; 16 Sep 2005 12:42:57 -0700 9, 24 -- Message-Id: {6.2.3.4.2.20050916124126.02908e80-at-mail.calweb.com} 9, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 9, 24 -- Date: Fri, 16 Sep 2005 12:43:00 -0700 9, 24 -- To: jfactor-at-ns.purchase.edu 9, 24 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 24 -- Subject: Re: [Microscopy] viaWWW: Digital camera for small animal 9, 24 -- Cc: microscopy-at-microscopy.com 9, 24 -- In-Reply-To: {200509161554.j8GFsOcl017735-at-ns.microscopy.com} 9, 24 -- References: {200509161554.j8GFsOcl017735-at-ns.microscopy.com} 9, 24 -- Mime-Version: 1.0 9, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 24 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 9, 24 -- X-Spam-Level: 9, 24 -- X-Spam-Status: No, score=-2.4 required=5.0 tests=AWL,BAYES_00 autolearn=ham 9, 24 -- version=3.0.3 ==============================End of - Headers==============================
The reason Geoff wants to put the heat block into the hood is probably the same reason why I would never polymerize resin outside the hood: fumes released during this process must be extremely toxic. And as far as finding a very small oven that would easily fit into a hood, this is not so easy. We bought the smallest we could find, but it is still more than 1 foot wide and deep, which takes too much room. We in fact have been using a second, smaller oven, that is really just a box fitted on top of a hot plate! It is very similar in design to the heat block Geoff wants to use. The temperature inside is very constant, the only drawback being that you need to calibrate the temperature control button. This only has to be done once. I think the idea of using heat blocks is very elegant, and I don't see why it shouldn't work with Eppendorf tubes or even Beem capsules. For flat embedding in moulds however I don't see how you would manage it.
Good thread, Geoff. My guess is that if nobody has tried this before, you should give it a shot and report to the list how it went!
Marc
On Friday, September 16, 2005, at 02:51 PM, paul_hazelton-at-umanitoba.ca wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Goeff } } You ask a question which is actually quite interesting, and applicable } on a number of levels. First, of course, is would a block work. I } will } assume you mean something like the sand blocks we use in the other (one } of my 2 non-EM homes) lab. As you note, there would be limitations, } but } the temperature control on all sand blocks I’ve ever worked with is a } lot better than any oven I’ve used. You just have to take the time to } set the temperature. As Tom Phillips noted, there are plenty of } examples of alternative systems for providing the polymerization } temperatures, so to join the chorus, I see no reason why not. And as } far as using the car engine, I even remember a book about cooking while } you drive which came out in the 70's, engine block potroast and all. } } The interesting part of the question as I see it is: why would you want } to put the block into a hood. Do you mean a fume hood, so you could } use } the block for an intermediate step in infiltration, with low } temperature } heating to assist in driving off transitional solvents? If so, it is } an } interesting idea, one worth some thought. Could be quite useful. } } Alternatively, do you mean a laminar flow hood for containment at a } BSL-2 level or higher. This is the most interesting potential } application. Those of us who work with emerging diseases groups, or } with infectious pathogens at the BSL-3 or BSL-4 levels are confronted } with a number of safety issues that this concept could address. Some } of } my collaborators work at higher levels. They fix with a modification } of } Karnovsky’s fix (we use 2% paraform/2%glut in cacodylate). But their } safety people will not let them take the material out of containment } for } further processing until the samples have been in the fixative for 30 } days. I feel this may lead to some deterioration of the samples, and } that there is no evidence that the pathogens are not inactivated in } hours, and so do not like this. But safety people will not let them do } otherwise. } } There are too many other things to do these days for me to give up 4-6 } hour blocks to go work in containment, so I’m not too keen on taking } spacesuit training - it would be fun and really interesting, but there } is just not enough time. Your question raises a lot of ideas which can } address the problems of processing, permit good technique in } processing, } and meet the demands on the biosafety level. } } Paul } } } Paul R. Hazelton, PhD } Electron Microscope Unit } University of Manitoba } Department of Medical Microbiology } 531 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 0W3 } e-mail: paul_hazelton-at-umanitoba.ca } Phone:204-789-3313 } Pager:204-931-9354 } Cell:204-781-1502 } Fax:204-789-3926 } } } ==============================Original } Headers============================== } 9, 19 -- From paul_hazelton-at-umanitoba.ca Fri Sep 16 13:50:14 2005 } 9, 19 -- Received: from electra.cc.umanitoba.ca } (electra.cc.umanitoba.ca [130.179.16.23]) } 9, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j8GIoE2H001127 } 9, 19 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 13:50:14 } -0500 } 9, 19 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca } [140.193.11.160]) } 9, 19 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id } j8GIo6fh004532; } 9, 19 -- Fri, 16 Sep 2005 13:50:06 -0500 (CDT) } 9, 19 -- Message-ID: {432B13DF.C669EB0A-at-umanitoba.ca} } 9, 19 -- Date: Fri, 16 Sep 2005 13:50:08 -0500 } 9, 19 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} } 9, 19 -- X-Mailer: Mozilla 4.78 [en] (Win98; U) } 9, 19 -- X-Accept-Language: en,pdf } 9, 19 -- MIME-Version: 1.0 } 9, 19 -- To: Geoffrey_Williams-at-brown.edu } 9, 19 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} } 9, 19 -- Subject: Re: [Microscopy] Curing TEM Epoxy in Heat Blocks? } 9, 19 -- References: {200509161506.j8GF6xh3016357-at-ns.microscopy.com} } 9, 19 -- Content-Type: text/plain; charset=iso-8859-1 } 9, 19 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers============================== } }
-- Marc Pypaert Department of Cell Biology Center for Cell and Molecular Imaging Ludwig Institute for Cancer Research Yale University School of Medicine 333 Cedar Street, PO Box 208002 New Haven, CT 06520-8002 TEL 203-785 3681 FAX 203-785 7446
==============================Original Headers============================== 10, 19 -- From marc.pypaert-at-yale.edu Fri Sep 16 14:44:41 2005 10, 19 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48]) 10, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8GJifEf012600 10, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 14:44:41 -0500 10, 19 -- Received: from yale.edu (net234-111.med.yale.edu [130.132.234.111]) 10, 19 -- by biomed.med.yale.edu (PMDF V6.1-1 #30532) 10, 19 -- with ESMTP id {01LT3RHRAM8O00LBGG-at-biomed.med.yale.edu} for 10, 19 -- Microscopy-at-microscopy.com; Fri, 16 Sep 2005 15:44:58 -0400 (EDT) 10, 19 -- Date: Fri, 16 Sep 2005 15:44:39 -0400 10, 19 -- From: Marc Pypaert {marc.pypaert-at-yale.edu} 10, 19 -- Subject: Re: [Microscopy] Re: Curing TEM Epoxy in Heat Blocks? 10, 19 -- In-reply-to: {200509161851.j8GIpEHm002838-at-ns.microscopy.com} 10, 19 -- To: paul_hazelton-at-umanitoba.ca, Microscopy-at-microscopy.com 10, 19 -- Message-id: {517B1438-26EA-11DA-8128-0030659833B4-at-yale.edu} 10, 19 -- MIME-version: 1.0 (Apple Message framework v553) 10, 19 -- X-Mailer: Apple Mail (2.553) 10, 19 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1; delsp=yes 10, 19 -- Content-Transfer-Encoding: 8bit 10, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8GJifEf012600 ==============================End of - Headers==============================
When our current was being designed, I specified one bench with an awning hood to vent fumes from solvent dishes, paraffin baths and embedding ovens. The 30" deep bench has a 3" gap between the backsplash and wall. The ovens are beneath the bench and their fumes are pulled up behind the backsplash. The stainless steel awning is 28" above the bench with a plexiglas skirt extending 4" below the awning to increase face flow.
A heat block is likely much cheaper than remodeling one's lab.
Glen On Sep 16, 2005, at 12:46 PM, marc.pypaert-at-yale.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Paul, } } The reason Geoff wants to put the heat block into the hood is } probably the same reason why I would never polymerize resin } outside the hood: fumes released during this process must be } extremely toxic. } And as far as finding a very small oven that would easily fit into } a hood, this is not so easy. We bought the smallest we could find, } but it is still more than 1 foot wide and deep, which takes too } much room. We in fact have been using a second, smaller oven, } that is really just a box fitted on top of a hot plate! It is very } similar } in design to the heat block Geoff wants to use. The temperature } inside is very constant, the only drawback being that you need } to calibrate the temperature control button. This only has to be } done once. I think the idea of using heat blocks is very elegant, } and I don't see why it shouldn't work with Eppendorf tubes or } even Beem capsules. For flat embedding in moulds however I } don't see how you would manage it. } } Good thread, Geoff. My guess is that if nobody has tried this } before, you should give it a shot and report to the list how it went! } } Marc } } } On Friday, September 16, 2005, at 02:51 PM, paul_hazelton-at-umanitoba.ca } wrote: } } } } } } } } } } --------------------------------------------------------------------- } } -- } } ----- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } -- } } ----- } } } } Goeff } } } } You ask a question which is actually quite interesting, and } } applicable } } on a number of levels. First, of course, is would a block work. I } } will } } assume you mean something like the sand blocks we use in the other } } (one } } of my 2 non-EM homes) lab. As you note, there would be limitations, } } but } } the temperature control on all sand blocks I’ve ever worked with is a } } lot better than any oven I’ve used. You just have to take the } } time to } } set the temperature. As Tom Phillips noted, there are plenty of } } examples of alternative systems for providing the polymerization } } temperatures, so to join the chorus, I see no reason why not. And as } } far as using the car engine, I even remember a book about cooking } } while } } you drive which came out in the 70's, engine block potroast and all. } } } } The interesting part of the question as I see it is: why would you } } want } } to put the block into a hood. Do you mean a fume hood, so you could } } use } } the block for an intermediate step in infiltration, with low } } temperature } } heating to assist in driving off transitional solvents? If so, it is } } an } } interesting idea, one worth some thought. Could be quite useful. } } } } Alternatively, do you mean a laminar flow hood for containment at a } } BSL-2 level or higher. This is the most interesting potential } } application. Those of us who work with emerging diseases groups, or } } with infectious pathogens at the BSL-3 or BSL-4 levels are confronted } } with a number of safety issues that this concept could address. Some } } of } } my collaborators work at higher levels. They fix with a modification } } of } } Karnovsky’s fix (we use 2% paraform/2%glut in cacodylate). But their } } safety people will not let them take the material out of containment } } for } } further processing until the samples have been in the fixative for 30 } } days. I feel this may lead to some deterioration of the samples, and } } that there is no evidence that the pathogens are not inactivated in } } hours, and so do not like this. But safety people will not let } } them do } } otherwise. } } } } There are too many other things to do these days for me to give up } } 4-6 } } hour blocks to go work in containment, so I’m not too keen on taking } } spacesuit training - it would be fun and really interesting, but } } there } } is just not enough time. Your question raises a lot of ideas } } which can } } address the problems of processing, permit good technique in } } processing, } } and meet the demands on the biosafety level. } } } } Paul } } } } } } Paul R. Hazelton, PhD } } Electron Microscope Unit } } University of Manitoba } } Department of Medical Microbiology } } 531 Basic Medical Sciences Building } } 730 William Avenue } } Winnipeg, Manitoba, Canada, R3E 0W3 } } e-mail: paul_hazelton-at-umanitoba.ca } } Phone:204-789-3313 } } Pager:204-931-9354 } } Cell:204-781-1502 } } Fax:204-789-3926 } } } } } } ==============================Original } } Headers============================== } } 9, 19 -- From paul_hazelton-at-umanitoba.ca Fri Sep 16 13:50:14 2005 } } 9, 19 -- Received: from electra.cc.umanitoba.ca } } (electra.cc.umanitoba.ca [130.179.16.23]) } } 9, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } j8GIoE2H001127 } } 9, 19 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 } } 13:50:14 } } -0500 } } 9, 19 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca } } [140.193.11.160]) } } 9, 19 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id } } j8GIo6fh004532; } } 9, 19 -- Fri, 16 Sep 2005 13:50:06 -0500 (CDT) } } 9, 19 -- Message-ID: {432B13DF.C669EB0A-at-umanitoba.ca} } } 9, 19 -- Date: Fri, 16 Sep 2005 13:50:08 -0500 } } 9, 19 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} } } 9, 19 -- X-Mailer: Mozilla 4.78 [en] (Win98; U) } } 9, 19 -- X-Accept-Language: en,pdf } } 9, 19 -- MIME-Version: 1.0 } } 9, 19 -- To: Geoffrey_Williams-at-brown.edu } } 9, 19 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} } } 9, 19 -- Subject: Re: [Microscopy] Curing TEM Epoxy in Heat Blocks? } } 9, 19 -- References: {200509161506.j8GF6xh3016357-at-ns.microscopy.com} } } 9, 19 -- Content-Type: text/plain; charset=iso-8859-1 } } 9, 19 -- Content-Transfer-Encoding: 8bit } } ==============================End of - } } Headers============================== } } } } } } } } -- } Marc Pypaert } Department of Cell Biology } Center for Cell and Molecular Imaging } Ludwig Institute for Cancer Research } Yale University School of Medicine } 333 Cedar Street, PO Box 208002 } New Haven, CT 06520-8002 } TEL 203-785 3681 } FAX 203-785 7446 } } } } ==============================Original } Headers============================== } 10, 19 -- From marc.pypaert-at-yale.edu Fri Sep 16 14:44:41 2005 } 10, 19 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu } [130.132.232.48]) } 10, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j8GJifEf012600 } 10, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 } 14:44:41 -0500 } 10, 19 -- Received: from yale.edu (net234-111.med.yale.edu } [130.132.234.111]) } 10, 19 -- by biomed.med.yale.edu (PMDF V6.1-1 #30532) } 10, 19 -- with ESMTP id {01LT3RHRAM8O00LBGG-at-biomed.med.yale.edu} for } 10, 19 -- Microscopy-at-microscopy.com; Fri, 16 Sep 2005 15:44:58 } -0400 (EDT) } 10, 19 -- Date: Fri, 16 Sep 2005 15:44:39 -0400 } 10, 19 -- From: Marc Pypaert {marc.pypaert-at-yale.edu} } 10, 19 -- Subject: Re: [Microscopy] Re: Curing TEM Epoxy in Heat } Blocks? } 10, 19 -- In-reply-to: {200509161851.j8GIpEHm002838-at-ns.microscopy.com} } 10, 19 -- To: paul_hazelton-at-umanitoba.ca, Microscopy-at-microscopy.com } 10, 19 -- Message-id: {517B1438-26EA-11DA-8128-0030659833B4-at-yale.edu} } 10, 19 -- MIME-version: 1.0 (Apple Message framework v553) } 10, 19 -- X-Mailer: Apple Mail (2.553) } 10, 19 -- Content-type: text/plain; format=flowed; } charset=ISO-8859-1; delsp=yes } 10, 19 -- Content-Transfer-Encoding: 8bit } 10, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j8GJifEf012600 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 26 -- From glenmac-at-u.washington.edu Fri Sep 16 15:09:52 2005 6, 26 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135]) 6, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8GK9q3Y027606 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 15:09:52 -0500 6, 26 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.9]) 6, 26 -- by mxout5.cac.washington.edu (8.13.4+UW05.04/8.13.4+UW05.07) with ESMTP id j8GK9pAo011663 6, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 13:09:51 -0700 6, 26 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 6, 26 -- (authenticated authid=glenmac) 6, 26 -- by smtp.washington.edu (8.13.4+UW05.04/8.13.4+UW05.07) with ESMTP id j8GK9pLp014863 6, 26 -- (version=TLSv1/SSLv3 cipher=RC4-SHA bits=128 verify=NOT) 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 13:09:51 -0700 6, 26 -- Mime-Version: 1.0 (Apple Message framework v734) 6, 26 -- In-Reply-To: {200509161946.j8GJkHd2017804-at-ns.microscopy.com} 6, 26 -- References: {200509161946.j8GJkHd2017804-at-ns.microscopy.com} 6, 26 -- Content-Type: text/plain; charset=WINDOWS-1252; delsp=yes; format=flowed 6, 26 -- Message-Id: {FA373827-E336-449E-B415-E8231A19BDB7-at-u.washington.edu} 6, 26 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 6, 26 -- Subject: Re: [Microscopy] Curing TEM Epoxy in Heat Blocks? 6, 26 -- Date: Fri, 16 Sep 2005 13:09:14 -0700 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- X-Mailer: Apple Mail (2.734) 6, 26 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='IP_HTTP_ADDR 0, __C230066_P1_5 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BADTHINGS 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_SPAMMER_ADDRESS_5 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0' 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8GK9q3Y027606 ==============================End of - Headers==============================
Geoff, Ladd Research makes a Dri Block Oven for curing Beem caps and flat molds. We used one regularly some time ago. It is compact and the temperature was stable. Frank
At 11:06 AM 9/16/2005, you wrote:
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==============================Original Headers============================== 7, 26 -- From macaluso-at-aecom.yu.edu Fri Sep 16 16:03:40 2005 7, 26 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) 7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8GL3d6A004112 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 16:03:40 -0500 7, 26 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) 7, 26 -- by mailgw.aecom.yu.edu (8.12.11/8.12.11) with SMTP id j8GL3bZr013560; 7, 26 -- Fri, 16 Sep 2005 17:03:37 -0400 7, 26 -- Received: from post.aecom.yu.edu ([129.98.1.100]) 7, 26 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2005091617033624720 7, 26 -- ; Fri, 16 Sep 2005 17:03:36 -0400 7, 26 -- Received: from aif1.aecom.yu.edu (aif1.aif.aecom.yu.edu [129.98.30.104]) 7, 26 -- by post.aecom.yu.edu (Postfix) with ESMTP id E8A402FCB; 7, 26 -- Fri, 16 Sep 2005 17:03:36 -0400 (EDT) 7, 26 -- Message-Id: {6.2.3.4.0.20050916165755.037df6e8-at-mailserver.aecom.yu.edu} 7, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 7, 26 -- Date: Fri, 16 Sep 2005 17:03:39 -0400 7, 26 -- To: Geoffrey_Williams-at-brown.edu 7, 26 -- From: Frank Macaluso {macaluso-at-aecom.yu.edu} 7, 26 -- Subject: Re: [Microscopy] Curing TEM Epoxy in Heat Blocks? 7, 26 -- Cc: "Microscopy Listserver" {microscopy-at-microscopy.com} 7, 26 -- In-Reply-To: {200509161506.j8GF6pb7015964-at-ns.microscopy.com} 7, 26 -- References: {200509161506.j8GF6pb7015964-at-ns.microscopy.com} 7, 26 -- Mime-Version: 1.0 7, 26 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j8GL3d6A004112 ==============================End of - Headers==============================
Does anyone have experience with deriving roughness information from SEM images? I presume you need to use stereo pairs to get 3D information from a rough substrate.
I would be especially interested if anyone had a free/cheap software package that would handle this task.
Thanks, Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
It appears to me that the problem to be solved is to find a way to polymerize resin but protect staff personnel from the fumes that are given off during heating yet find a space-saving solution.
A simple approach would be to only use sealed molds such as Eppendorf tubes and BEEM capsules. The oven can thus be placed anywhere in the lab. However, I do know that many people prefer the ease and lower cost of re-useable flat molds.
I have been experimenting with polymerizing resins using a microwave oven. With variable results. However, with some formulations of resin it is possible to polymerize in a flat mold in less than 2hr.
I have used a laboratory-grade microwave connected to an exhaust duct (which is very convenient) and also with a regular kitchen microwave. The end result is, if the resin is going to polymerize, the process will work in either machine.
Not all resin recipes work and even fewer of them can be polymerized in a flat mold. It may be worth giving this approach a try.
The best part of sing the microwave is that the exhaust duct allows us to place the machine far from the chemical extractor hoods. Connecting a regular oven to an exhaust duct may also be a reasonably effective solution. Our convection oven has a wide duct in the top to which metal ducting, similar to that connected to household clothes dryers, can be connected.
Best regards,
Paul Webster
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
==============================Original Headers============================== 13, 16 -- From PWebster-at-hei.org Fri Sep 16 18:01:02 2005 13, 16 -- Received: from hi0sml1.hei.org (watchhouse.hei.org [63.194.44.2]) 13, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8GN1139022099 13, 16 -- for {Microscopy-at-Microscopy.Com} ; Fri, 16 Sep 2005 18:01:01 -0500 13, 16 -- Received: from 10.10.41.3 ([10.10.41.3]) by hi0sml1.hei.org ([63.194.44.59]) with Microsoft Exchange Server HTTP-DAV ; 13, 16 -- Fri, 16 Sep 2005 23:01:00 +0000 13, 16 -- User-Agent: Microsoft-Entourage/11.1.0.040913 13, 16 -- Date: Fri, 16 Sep 2005 15:56:48 -0700 13, 16 -- Subject: Re: Curing TEM Epoxy in Heat Blocks? 13, 16 -- From: "Webster, Paul" {PWebster-at-hei.org} 13, 16 -- To: {Microscopy-at-Microscopy.Com} 13, 16 -- Message-ID: {BF509BC0.5709%PWebster-at-hei.org} 13, 16 -- Mime-version: 1.0 13, 16 -- Content-type: text/plain; 13, 16 -- charset="US-ASCII" 13, 16 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Hi all I am wondering reading these e-mails, does anyone really know how noxious the fumes are that are released from an embedding oven? We too are pressed for space in the hood so I've moved the embedding oven into a not heavily populated corner in a large lab. We polymerize LR White and epoxy resin blocks-5-10 blocks worth maybe once a week at most. I can't smell any fumes in there ( unlike the mercaptoethanol or ETT the molecular biologists regularly use). Am I exposing a room full of people to something bad? Is it the quantity of blocks that one needs to worry about? The microwave is great for processing (hooked up to the fume hood via the duct) but I don't ant to baby sit it for 2 hours to get a perfectly hardened epoxy flat block. Safety conscious in CA JoAnn Buchanan
==============================Original Headers============================== 3, 15 -- From redhair-at-stanford.edu Fri Sep 16 18:30:14 2005 3, 15 -- Received: from smtp2.Stanford.EDU (smtp2.Stanford.EDU [171.67.16.125]) 3, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j8GNUEZm030777 3, 15 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 18:30:14 -0500 3, 15 -- Received: from lil-mama.stanford.edu (ssmith-pbdsl1.Stanford.EDU [171.66.197.50]) 3, 15 -- by smtp2.Stanford.EDU (8.12.11/8.12.11) with ESMTP id j8GNU2VY007860 3, 15 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2005 16:30:12 -0700 3, 15 -- Message-Id: {6.2.3.4.2.20050916161514.04fdae48-at-redhair.pobox.stanford.edu} 3, 15 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 3, 15 -- Date: Fri, 16 Sep 2005 16:29:59 -0700 3, 15 -- To: {microscopy-at-microscopy.com} 3, 15 -- From: JoAnn Buchanan {redhair-at-stanford.edu} 3, 15 -- Subject: Curing TEM Epoxy in Heat Blocks? 3, 15 -- Mime-Version: 1.0 3, 15 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Yes, you can use stereo pairs to calculate a surface profile and from there calculate roughness parameters. We have a module for our analySIS software that accomplishes this. If you want more information, please contact me by email.
There are certain limitations to what you can do with this technique. The z-resolution depends on a number of factors, the most important of which is the tilt angle. For typical tilt angles of 6 - 10 degrees, the resolution is roughly 1/10th of the lateral resolution (you can calculate that by multiplying the lateral resolution with the tan of the stereo angle). For example: If your stereo images have a resolution of 1 micron (1mm x 1mm field of view and an image resolution of 1000 x 1000 pixels), your z-resolution will be on the order of 10 microns. In order to evaluate if the technique will do what you want, you need the following information: required field of view, image resolution, stereo angle.
Let me know if you need further info.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca] Sent: Friday, September 16, 2005 4:53 PM To: Mike Bode
Hi everyone,
Does anyone have experience with deriving roughness information from SEM images? I presume you need to use stereo pairs to get 3D information from a rough substrate.
I would be especially interested if anyone had a free/cheap software package that would handle this task.
Thanks, Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
In a message dated 9/16/05 6:50:20 PM, Daniel.Salamon-at-nrc-cnrc.gc.ca writes:
} Does anyone have experience with deriving roughness information from SEM } images? I presume you need to use stereo pairs to get 3D information } from a rough substrate. } } I would be especially interested if anyone had a free/cheap software } package that would handle this task.
I don't know of any free stuff. The least expensive is probably the Fovea Pro software from Reindeer (www.ReindeerGraphics.com), which has a demo version you can download that both fuses stereo pairs and performs a variety of surface roughness measurements. Several other companies offer routines that derive elevation from stereo pairs, but few also perform roughness measurements. The most versatile, complete and probably accurate program I've seen is the Alicona package (www.alicona.com), which has a demo version you can download. But it's a long way from free.
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Mike Bode described a way of estimating the vertical resolution that can be achieved by using stereo pairs. His trigonometric calculation predicts that the practical vertical resolution is 10x worse than the lateral resolution, for tilt angles of 6-10 degrees. I wonder whether users of various SEM measurement tools have the same experience in actual practice. And I wonder what the observed limits of vertical resolution are for the highest resolution FE-SEMs. regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
----- Original Message ----- From: Mike.Bode-at-soft-imaging.net To: donc-at-asmicro.com Sent: Friday, September 16, 2005 6:34 PM Subject: [a] [Microscopy] RE: SEM roughness measurement
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Hello Daniel,
Yes, you can use stereo pairs to calculate a surface profile and from there calculate roughness parameters. We have a module for our analySIS software that accomplishes this. If you want more information, please contact me by email.
There are certain limitations to what you can do with this technique. The z-resolution depends on a number of factors, the most important of which is the tilt angle. For typical tilt angles of 6 - 10 degrees, the resolution is roughly 1/10th of the lateral resolution (you can calculate that by multiplying the lateral resolution with the tan of the stereo angle). For example: If your stereo images have a resolution of 1 micron (1mm x 1mm field of view and an image resolution of 1000 x 1000 pixels), your z-resolution will be on the order of 10 microns. In order to evaluate if the technique will do what you want, you need the following information: required field of view, image resolution, stereo angle.
Let me know if you need further info.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca] Sent: Friday, September 16, 2005 4:53 PM To: Mike Bode Subject: [Microscopy] SEM roughness measurement
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Hi everyone,
Does anyone have experience with deriving roughness information from SEM images? I presume you need to use stereo pairs to get 3D information from a rough substrate.
I would be especially interested if anyone had a free/cheap software package that would handle this task.
Thanks, Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4